Molecular Biology This is an OPML file. It can be used to export all the MedWorm RSS feeds on this topic into your personal RSS reader (usually you have to save this file to your own computer before clicking on an Import OPML command in your own feed reader to upload the file which will then import all the feeds) or it can be used by webmasters to integrate MedWorm feeds with their own website. This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

This page shows you the most recent publications within this specialty of the MedWorm directory.

Orthogonally protected D-galactosamine thioglycoside building blocks via highly regioselective, double serial and double parallel inversions of [small beta]-D-thiomannoside
Org. Biomol. Chem., 2013, Advance ArticleDOI: 10.1039/C3OB40935J, PaperMadhu Emmadi, Suvarn S. KulkarniAn efficient route for the synthesis of orthogonally protected D-galactosamine thioglycosides from D-mannose and its application to the synthesis of the rare disaccharide moiety of the zwitterionic polysaccharide of Bacteroides fragilis and Tn antigen is described.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - June 17, 2013 Category: Molecular Biology Authors: Madhu Emmadi Source Type: research

The origin of enantioselectivity in the L-threonine-derived phosphine-sulfonamide catalyzed aza-Morita-Baylis-Hillman reaction: effects of the intramolecular hydrogen bonding
Org. Biomol. Chem., 2013, Advance ArticleDOI: 10.1039/C3OB40144H, PaperRichmond Lee, Fangrui Zhong, Bin Zheng, Yuezhong Meng, Yixin Lu, Kuo-Wei HuangThe intramolecular N-HO H-bond was identified to be crucial in inducing a high degree of stereochemical control in both the enolate addition to imine and the subsequent proton transfer step.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - June 17, 2013 Category: Molecular Biology Authors: Richmond Lee Source Type: research

Incorporation of a calixarene-based glucose functionalised bolaamphiphile into lipid bilayers for multivalent lectin recognition
Org. Biomol. Chem., 2013, Advance ArticleDOI: 10.1039/C3OB40732B, PaperS. Aleandri, A. Casnati, L. Fantuzzi, G. Mancini, G. Rispoli, F. SansoneThe incorporation of a new glucosylated bolaamphiphile into DOPC liposomes rigidifies the lipophilic bilayer, thus reducing leakage from the internal aqueous phase, and allows a multivalent interaction with Concanavalin A.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - June 17, 2013 Category: Molecular Biology Authors: S. Aleandri Source Type: research

C-H[middle dot][middle dot][middle dot]O Non-Classical Hydrogen Bonding in the Stereomechanics of Organic Transformations: Theory and Recognition
Org. Biomol. Chem., 2013, Accepted ManuscriptDOI: 10.1039/C3OB40828K, PerspectiveRyne C. Johnston, Paul Ha-Yeon CheongThis manuscript describes the role of non-classical hydrogen bonds (NCHBs), specifically C-H[middle dot][middle dot][middle dot]O interactions, in modern synthetic organic transformations. Our goal is to point out the seminal examples where C-H[middle dot][middle dot][middle dot]O interactions...The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - June 17, 2013 Category: Molecular Biology Authors: Ryne C. Johnston Source Type: research

Tris(2-aminoethyl)amine based tripodal urea receptors for oxalate: encapsulation of staggered vs. planar conformers
Org. Biomol. Chem., 2013, Advance ArticleDOI: 10.1039/C3OB41071D, CommunicationPurnandhu Bose, Ranjan Dutta, Pradyut GhoshSimple tris(2-aminoethyl)amine (TREN) based tripodal urea receptors are investigated for the encapsulation of divalent oxalate (C2O42-) in a semi-aqueous medium.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - June 17, 2013 Category: Molecular Biology Authors: Purnandhu Bose Source Type: research

The Cascade Radical Cyclisation Approach to Prenylated Alkaloids: Synthesis of Stephacidin A and Notoamide B
Org. Biomol. Chem., 2013, Accepted ManuscriptDOI: 10.1039/C3OB40979A, PaperN S Simpkins, Louise Male, Ilias Pavlakos, Michael WellerA strategy for the synthesis of members of the prenylated indole alkaloid family is described, which involves a radical cascade process of an appropriately substituted diketopiperazine (DKP) core structure. Several...The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - June 17, 2013 Category: Molecular Biology Authors: N S Simpkins Source Type: research

Synthesis and reactivity of 5-polyfluoroalkyl-5-deazaalloxazines
Org. Biomol. Chem., 2013, Accepted ManuscriptDOI: 10.1039/C3OB26837C, PaperSergii Dudkin, Viktor Iaroshenko, Vyacheslav Sosnovskikh, Andrey A Tolmachev, Peter LangerReaction of 6-arylamino-1,3-dialkyluracils with anhydrides of polyfluorocarboxylic acids in the presence of pyridine and subsequent cyclization with concentrated H2SO4 gave the corresponding 1,3-dialkyl-5-(polyfluoroalkyl)pyrimido[4,5-b]quinoline-2,4(1H,3H)-diones (5-polyfluoroalkyl-5-deazaalloxazines). The reactivity of these compounds against...The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - June 17, 2013 Category: Molecular Biology Authors: Sergii Dudkin Source Type: research

Directed studies towards the total synthesis of (+)-13-deoxytedanolide: simple and convenient synthesis of the C8-C16 fragment
Org. Biomol. Chem., 2013, Advance ArticleDOI: 10.1039/C3OB40674A, PaperSebastien Meiries, Alexandra Bartoli, Melanie Decostanzi, Jean-Luc Parrain, Laurent CommeirasA straightforward synthesis for enantioenriched C8-C16 south part of (+)-13-deoxytedanolide has been reported.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - June 17, 2013 Category: Molecular Biology Authors: Sebastien Meiries Source Type: research

Quick and reversible photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside in a DNA triplex
Org. Biomol. Chem., 2013, Advance ArticleDOI: 10.1039/C3OB40915E, CommunicationKenzo Fujimoto, Hiroki Yoshinaga, Yasumasa Yoshio, Takashi Sakamoto3-Cyanovinylcarbazole nucleoside (CNVK), which can quickly photocrosslink to the pyrimidine base in the DNA double strand, was incorporated into a triplex forming oligonucleotides (TFO(s)) and the photoreactivity of the TFOs were evaluated.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - June 17, 2013 Category: Molecular Biology Authors: Kenzo Fujimoto Source Type: research

Extolling the benefits of molecular therapeutic lipidation
Mol. BioSyst., 2013, Advance ArticleDOI: 10.1039/C3MB70147F, Review ArticleMiriam Avadisian, Patrick T. GunningThe conjugation of drug or molecular recognition motif to a hydrophobic fatty entity, for purpose of drug-membrane localization, has been a molecular strategy utilized for targeted inhibition of pathways involved in diseased cells.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Mol. BioSyst. latest articles)
Source: RSC - Mol. BioSyst. latest articles - June 17, 2013 Category: Molecular Biology Authors: Miriam Avadisian Source Type: research

Structural features of falcipain-3 inhibitors: an in silico study
Mol. BioSyst., 2013, Advance ArticleDOI: 10.1039/C3MB70105K, PaperJinghui Wang, Feng Li, Yan Li, Yinfeng Yang, Shuwei Zhang, Ling YangFalcipain-3, the major cysteine hemoglobinase from the human malaria parasite Plasmodium falciparum, is critical for parasite development and is considered as a promising chemotherapeutic target.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Mol. BioSyst. latest articles)
Source: RSC - Mol. BioSyst. latest articles - June 17, 2013 Category: Molecular Biology Authors: Jinghui Wang Source Type: research

The Indirect Activation Model of Mitochondrial Outer Membrane Permeabilisation (MOMP) Initiation requires a Trade-off between Robustness in Absence, and Sensitivity in Presence of Stress
Mol. BioSyst., 2013, Accepted ManuscriptDOI: 10.1039/C3MB70076C, PaperAndreas U Lindner, Jochen HM Prehn, Heinrich J HuberMitochondrial outer membrane permeabilisation (MOMP) is a hallmark of apoptosis and controlled by antagonising members of the BCL 2 protein family. However, whether the effector proteins for MOMP, BAX and...The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Mol. BioSyst. latest articles)
Source: RSC - Mol. BioSyst. latest articles - June 17, 2013 Category: Molecular Biology Authors: Andreas U Lindner Source Type: research

A novel mechanism for antiglycative action of limonene through stabilization of protein conformation
Mol. BioSyst., 2013, Accepted ManuscriptDOI: 10.1039/C3MB00020F, PaperMadhav M. Joglekar, Shrimant N. Panaskar, Ashok D. Chougale, Mahesh J Kulkarni, Akalpita U. ArvindekarInhibition of protein glycation is known to ameliorate secondary complications in diabetes. In the present study antiglycative properties of limonene; a natural product, was evaluated using BSA as a model...The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Mol. BioSyst. latest articles)
Source: RSC - Mol. BioSyst. latest articles - June 17, 2013 Category: Molecular Biology Authors: Madhav M. Joglekar Source Type: research

Physiological Distal Drift in Rat Molars Contributes to Acellular Cementum Formation
In conclusion, the physiological distal drift in rat molars appears to be largely driven by the occlusal force and also affects the formation of acellular cementum. These findings provide additional direct evidence for an important role of occlusal forces in tooth migration. Anat Rec, 2013. © 2013 Wiley Periodicals, Inc. (Source: The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology)
Source: The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology - June 17, 2013 Category: Molecular Biology Authors: Shinobu Tsuchiya, Masahiro Tsuchiya, Takashi Nishioka, Osamu Suzuki, Yasuyuki Sasano, Kaoru Igarashi Tags: Full Length Article Source Type: research

Teaching Complete Evolutionary Stories Increases Learning
Many students have difficulty understanding and explaining how evolution operates. In search of better ways to teach the subject, researchers at Michigan State University developed complete evolutionary case studies spanning the gamut from the molecular changes underlying an evolving characteristic to their genetic consequences and effects in populations. The researchers, Peter J. T. White, James J. Smith, and Merle K. Heidemann, then incorporated two of the scenarios into a cellular and molecular biology course taught to undergraduates at the university's Lyman Briggs College. When the students' understanding was tested, ...
Source: BioScience Press Releases - June 17, 2013 Category: Biology Authors: Timothy M. Beardsley Editor in Chief, BioScience American Insitute of Biological Sciences (AIBS) 1900 Campus Commons Drive, Suite 200 Reston, VA 20191 703-674-2500 x326 tbeardsley at aibs.org www.aibs.org Source Type: news

Alexandre Afranio Peixoto (1963-2013)
(Source: Genetics and Molecular Biology)
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Doralice Maria Cella (1954-2013)
(Source: Genetics and Molecular Biology)
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Genetic differences between Chibcha and Non-Chibcha speaking tribes based on mitochondrial DNA (mtDNA) haplogroups from 21 Amerindian tribes from Colombia
We analyzed the frequency of four mitochondrial DNA haplogroups in 424 individuals from 21 Colombian Amerindian tribes. Our results showed a high degree of mtDNA diversity and genetic heterogeneity. Frequencies of mtDNA haplogroups A and C were high in the majority of populations studied. The distribution of these four mtDNA haplogroups from Amerindian populations was different in the northern region of the country compared to those in the south. Haplogroup A was more frequently found among Amerindian tribes in northern Colombia, while haplogroup D was more frequent among tribes in the south. Haplogroups A, C and D have cl...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

MHC Class II haplotypes of Colombian Amerindian tribes
We analyzed 1041 individuals belonging to 17 Amerindian tribes of Colombia, Chimila, Bari and Tunebo (Chibcha linguistic family), Embera, Waunana (Choco linguistic family), Puinave and Nukak (Maku-Puinave linguistic families), Cubeo, Guanano, Tucano, Desano and Piratapuyo (Tukano linguistic family), Guahibo and Guayabero (Guayabero Linguistic Family), Curripaco and Piapoco (Arawak linguistic family) and Yucpa (Karib linguistic family). for MHC class II haplotypes (HLA-DRB1, DQA1, DQB1). Approximately 90% of the MHC class II haplotypes found among these tribes are haplotypes frequently encountered in other Amerindian tribes...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Reconstruction of major maternal and paternal lineages of the Cape Muslim population
The earliest Cape Muslims were brought to the Cape (Cape Town -South Africa) from Africa and Asia from 1652 to 1834. They were part of an involuntary migration of slaves, political prisoners and convicts, and they contributed to the ethnic diversity of the present Cape Muslim population of South Africa. The history of the Cape Muslims has been well documented and researched however no in-depth genetic studies have been undertaken. The aim of the present study was to determine the respective African, Asian and European contributions to the mtDNA (maternal) and Y-chromosomal (paternal) gene pool of the Cape Muslim population...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Association between osteoprotegerin gene polymorphisms and cardiovascular disease in type 2 diabetic patients
This study provides evidence that the C allele of the T950C polymorphism is associated with increased risk of CVD in diabetic patients. However, well-designed prospective studies with a larger sample size are needed to validate these results. (Source: Genetics and Molecular Biology)
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Non-detection of Epstein-Barr virus and human papillomavirus in a region of high gastric cancer risk indicates a lack of a role for these viruses in gastric carcinomas
Gastric mucosa tissue was collected from patients with gastroduodenal diseases in a region of norrteastern China showing a high risk of gastric cancer incidence. The presence of EBV and HPV were assayed to investigate the relationship between gastric carcinomas and virus infection. Neither EBV nor HPV DNA was detected in tissue from the patients. The role of EBV and HPV in gastric cancer is not well understood and still needs to be clarified. (Source: Genetics and Molecular Biology)
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Comparison of the effectiveness of microsatellites and SNP panels for genetic identification, traceability and assessment of parentage in an inbred Angus herd
During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires a marker set with enough power to identify individuals and their parents. Genetic diagnostics generally include the analysis of related animals. In this work, the degree of information provided by SNPs for a consanguineous herd of cattle was compared with that provided by STRs. Thirty-six closely related Angus...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

New molecular evidence supports the species status of Kaempfer's Woodpecker (Aves, Picidae)
Kaempfer's Woodpecker (Celeus obrieni) is the only species of the genus Celeus endemic to Brazil. The description of this taxon as a subspecies of the Rufous-headed Woodpecker (Celeus spectabilis) was based on a single specimen. While C. obrieni and C. spectabilis are now considered separate species based on morphological and limited molecular evidence, no study has critically tested the reciprocal monophyly and degree of evolutionary independence between these taxa with several specimens. Herein, fragments of the mitochondrial and nuclear DNA of three recently-collected specimens of C. obrieni were analyzed to evaluate th...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

A remarkable autosomal heteromorphism in Pseudoryzomys simplex 2n = 56; FN = 54-55 (Rodentia, Sigmodontinae)
Pseudoryzomys simplex, the false rice rat, is a monotypic genus of the Oryzomyini tribe (Sigmodontinae) distributed in part of Bolivia, Paraguay, Argentina and Brazil. Its diploid number has been described as 56 acrocentric chromosomes decreasing in size and no karyotype figure has been depicted. Herein, we present karyotypic data on P. simplex, including chromosome banding and molecular fluorescent in situ hybridization using telomeric sequences and the whole X-chromosome of its sister clade Holochilus brasiliensis (HBR) as probes. A case of remarkable autosomal heteromorphism due to the presence of a whole heterochromati...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Genetic parameters for five traits in Africanized honeybees using Bayesian inference
Heritability and genetic correlations for honey (HP) and propolis production (PP), hygienic behavior (HB), syrupcollection rate (SCR) and percentage of mites on adult bees (PMAB) of a population of Africanized honeybees were estimated. Data from 110 queen bees over three generations were evaluated. Single and multi-trait models were analyzed by Bayesian Inference using MTGSAM. The localization of the hive was significant for SCR and HB and highly significant for PP. Season-year was highly significant only for SCR. The number of frames with bees was significant for HP and PP, including SCR. The heritability estimates were 0...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease
In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in different plant tissues. Some of the genes were also analyzed by time-course real time RT-qPCR in response to infection by Phakopsora pachyrhizi, the causal agent of Asian soybean rust disease. Our findings indicate that the time of induction of a defense pathway is crucial in triggering the soybean resistance response to P. pachyrhizi. This is the first study to identify the soybean HyPRP group B family and to analyze disease-responsive GmHyPRP during infection by P. p...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Modeling and analysis of soybean (Glycine max. L) Cu/Zn, Mn and Fe superoxide dismutases
In this study, we describe structural models for soybean Cu/Zn SOD, Mn SOD and Fe SOD and provide insights into the molecular function of this metal-binding enzyme in improving tolerance to oxidative stress in plants. (Source: Genetics and Molecular Biology)
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Genetic diversity in a world germplasm collection of tall fescue
In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of polymorphic information content ranged from 0.627 to 0.840. In addition, the accessions analyzed by flow cytometry showed different ploidy levels (diploid, tetraploid, hexaploid and octaploid), placing in evidence that the world germplasm collection consisted of mul...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach
The OmlA protein is a virulence factor of Actinobacillus pleuropneumoniae, an important pathogen in pigs. The polymorphisms present in the omlA gene sequence of 15 reference serotypes of A. pleuropneumoniae and non-serotypable isolates were assessed to determine the possible evolutionary relationship among them and to validate the importance of this gene as a molecular marker for the characterization of this bacterium. Divergence among the 15 serotypes of A. pleuropneumoniae probably resulted initially from two major evolutionary events that led to subsequent differentiation into nine groups. This differentiation makes it ...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Endophytic bacterial diversity in banana 'Prata Anã' (Musa spp.) roots
The genetic diversity of endophytic bacteria in banana 'Prata Anã' roots was characterized. Two hundred and one endophytic bacteria were isolated, 151 of which were classified as Gram-positive and 50 as Gram-negative. No hypersensitivity response was observed in any of the isolates. The rep-PCR technique generated different molecular profiles for each primer set (REP, ERIC and BOX). Fifty readable loci were obtained and all of the fragments were polymorphic. Amplified ribosomal DNA restriction analysis (ARDRA) of the isolates based on cleavage with four restriction enzymes yielded 45 polymorphic bands and no monomorphic b...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Distribution of PLD and FagA, B, C and D genes in Corynebacterium pseudotuberculosis isolates from sheep and goats with caseus lymphadenitis
Caseous lymphadenits (CL) is a chronic and subclinical disease that affects goats and sheep and, consequently, causes economic losses, especially to small producers. The purpose of this study, through use of Polymerase Chain Reaction (PCR), was to verify the presence of virulence genes of phospholipase D (PLD), integral membrane protein (FagA), iron enterobactin transporter (FagB), ATP binding cytoplasmic membrane protein (FagC) and iron siderophore binding protein (FagD) in 168 isolates of C. pseudotuberculosis obtained from cases of caseous lymphadenitis in goats and sheep. FagA, FagB and PLD genes were detected in all 1...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Genotoxicity of Nicotiana tabacum leaves on Helix aspersa
In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet assay and the concentration of cytochrome P450 enzymes was estimated. Two groups of snails were studied: one fed on tobacco leaves and one fed on lettuce (Lactuca sativa L) leaves (control group). All of the snails received leaves (tobacco and lettuce leaves were the only food provided) and water ad libitum. Hemolymph cells were collected after 0, 24, 48 and 72 h. The Comet assay and micronucleus test showed that exposure to tob...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Identification of alternative transcripts of rat CD9 expressed by tumorigenic neural cell lines and in normal tissues
CD9 is the best-studied member of the tetraspanin family of transmembrane proteins. It is involved in various fundamental cellular processes and its altered expression is a characteristic of malignant cells of different origins. Despite numerous investigations confirming its fundamental role, the heterogeneity of CD9 or other tetraspanin proteins was considered only to be caused by posttranslational modification, rather than alternative splicing. Here we describe the first identification of CD9 transcript variants expressed by cell lines derived from fetal rat brain cells. Variant mRNA-B lacks a potential translation initi...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Comparison of HMOX1 expression and enzyme activity in blue-shelled chickens and brown-shelled chickens
Blue egg coloring is attributed to biliverdin derived from the oxidative degradation of heme through catalysis by heme oxygenase (HO). The pigment is secreted into the eggshell by the shell gland. There is uncertainty as to whether the pigment is synthesized in the shell gland or in other tissues. To investigate the site of pigment biosynthesis, the expression of heme oxygenase (decycling) 1 (HMOX1), a gene encoding HO, and HO activity in liver and spleen were compared between blue-shelled chickens (n=12) and brown-shelled chickens (n=12). There were no significant differences in HMOX1 expression and HO activity in these t...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Bioinformatics analysis of the gene expression profile in Bladder carcinoma
Bladder carcinoma, which has the ninth highest incidence among malignant tumors in the world, is a complex, multifactorial disease. The malignant transformation of bladder cells results from DNA mutations and alterations in gene expression levels. In this work, we used a bioinformatics approach to investigate the molecular mechanisms of bladder carcinoma. Biochips downloaded from the Gene Expression Omnibus (GEO) were used to analyze the gene expression profile in urinary bladder cells from individuals with carcinoma. The gene expression profile of normal genomes was used as a control. The analysis of gene expression revea...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The M. scutellaris fat body ESTs encoded proteins with roles in numerous physiological processes, including anti-oxidation, phosphorylation, metabolism, detoxification, transmembrane transport, intracellular transport, cell proliferation, p...
Source: Genetics and Molecular Biology - June 17, 2013 Category: Genetics & Stem Cells Source Type: research

Major grant funds UCSC researchers using big data to predict cancer outcomes
(University of California - Santa Cruz) Despite some successes, predicting cancer outcomes based on the molecular signatures in cancer cells remains a major challenge. A new effort, funded by the National Cancer Institute and led by researchers at the University of California, Santa Cruz, aims to clear several key roadblocks that have stymied progress in this field. (Source: EurekAlert! - Biology)
Source: EurekAlert! - Biology - June 17, 2013 Category: Biology Source Type: news

Inflammatory Process Induced by Carrageenan in Adjacent Tissue Triggers the Acute Inflammation in Deep Digital Flexor Tendon of Rats
In this study alterations were verified in the extracellular matrix (ECM) of the deep digital flexor tendon after two periods (12 and 24 hr) of induction inflammation in rat paw. Wistar rats were divided into three groups: those that received injection of 1% carrageenan; those that received 0.9% NaCl; and those that received no application. The tendon was divided into distal (d), proximal (p), and intermediate (i) regions. Biochemical analyses were performed and included non‐collagenous proteins (NCP), glycosaminoglycans (GAGs), hydroxyproline (HoPro) and metalloproteinases 2 and 9. Tissue sections were stained with tolu...
Source: The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology - June 17, 2013 Category: Molecular Biology Authors: Cristiano Pedrozo Vieira, Andrea Aparecida Aro, Flávia Ré Guerra, Letícia Prado Oliveira, Marcos Dos Santos Almeida, Edson Rosa Pimentel Tags: Full Length Article Source Type: research

Drosophila semaphorin2b is required for the axon guidance of a subset of embryonic neurons
COVER PHOTOGRAPH: Drosophila embryonic axons visualized for expression of Fasciclin II (red) and a transgene that marks sema‐phorin2b‐positive cells (green). semaphorin2b‐positive axons deviate from their normal Fasciclin II tracts in both the lateral and ventral direction in semaphorin2b mutants (upper right) compared to wildtype (upper left). From Drosophila semaphorin2b is Required for the Axon Guidance of a Subset of Embryonic Neurons, Mark M. Emerson, Jennifer B. Long, and David Van Vactor, Developmental Dynamics 242:861–873, 2013. (Source: Developmental Dynamics)
Source: Developmental Dynamics - June 17, 2013 Category: Molecular Biology Authors: Mark M. Emerson, Jennifer B. Long, David Van Vactor Tags: Cover Image Source Type: research

DD ArtPix
(Source: Developmental Dynamics)
Source: Developmental Dynamics - June 17, 2013 Category: Molecular Biology Tags: Art Pix Source Type: research

An LPA species (18:1 LPA) plays key roles in the self-amplification of spinal LPA production in the peripheral neuropathic pain model
Conclusion: These results suggest that 18:1 LPA is the predominant ligand responsible for LPA1 and LPA3 receptors-mediated amplification of LPA production through microglial activation. (Source: Molecular Pain)
Source: Molecular Pain - June 17, 2013 Category: Molecular Biology Authors: Lin MaJun NagaiJerold ChunHiroshi Ueda Source Type: research

Ras, Raf, and MAP Kinase in Melanoma
A growing understanding of the biology and molecular mechanisms of melanoma has led to the identification of a number of driver mutations for this aggressive tumor. The most common mutations affect signaling of the Ras/Raf/MAPK (mitogen-activated protein kinase) pathway. This review will focus on mutations in genes encoding proteins that play a role in the MAPK pathway and that have been implicated in melanoma biology, such as BRAF, NRAS, and MEK (MAPK kinase), and detail the current understanding of their role in melanoma progression from a molecular biology perspective. Furthermore, this review will also consider some ad...
Source: Advances in Anatomic Pathology - June 16, 2013 Category: Pathology Tags: Review Articles Source Type: research

Adiponectin protects Leydig cells against pro‐inflammatory cytokines by suppressing the nuclear factor‐kappaB signaling pathway
This article is protected by copyright. All rights reserved. (Source: FEBS Journal)
Source: FEBS Journal - June 16, 2013 Category: Research Authors: Ling Wu, Bufang Xu, Weimin Fan, Xiaobin Zhu, Guangyan Wang, Aijun Zhang Tags: Original Article Source Type: research

Determination of serum levels of imatinib mesylate in patients with chronic myeloid leukemia: validation and application of a new analytical method to monitor treatment compliance
CONCLUSION: The method developed in this study was successfully validated and is being efficiently used to measure imatinib concentrations in samples from chronic myeloid leukemia patients to check treatment compliance. The imatinib serum levels of patients achieving a major molecular response were significantly higher than those of patients who did not achieve this result. These results are thus consistent with published reports concerning other populations. (Source: Revista Brasileira de Hematologia e Hemoterapia)
Source: Revista Brasileira de Hematologia e Hemoterapia - June 15, 2013 Category: Hematology Source Type: research

Mol Med; +295 new citations
295 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Mol Med These pubmed results were generated on 2013/06/15PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Molecular Medicine)
Source: Molecular Medicine - June 15, 2013 Category: Molecular Biology Tags: Report Source Type: research

Impact of biological sex on the pathophysiology of the heart
Abstract The cardiovascular diseases are the leading causes of death in men and women in industrialised countries. While the impact of biological sex on cardiovascular pathophysiology has long been known, the sex‐specific mechanisms mediating these processes have progressed during the last years. This review aims at analysing the sex‐based differences in cardiac structure and function in adult mammals, and the sex‐based differences in the main molecular mechanisms involved in the response of the heart to pathological situations. It emerged from this review that the sex‐based difference is a variable that should be ...
Source: British Journal of Pharmacology - June 15, 2013 Category: Drugs & Pharmacology Authors: Loubina Fazal, Feriel Azibani, Nicolas Vodovar, Alain Cohen Solal, Claude Delcayre, Jane‐Lise Samuel Tags: Review Article Source Type: research

Binding mode prediction of biologically active compounds from plant Salvia Miltiorrhiza as integrase inhibitor.
Abstract Integrase (IN), an essential enzyme for HIV-1 replication, has been targeted in antiretroviral drug therapy. The emergence of HIV-1 variants clinically resistant to antiretroviral agents has lead to the development of alternative IN inhibitors. In the present work, binding modes of a high potent IN inhibitor, M522 and M532, within the catalytic binding site of wild type (WT) IN were determined using molecular docking calculation. Both M522 and M532 displayed similar modes of binding within the IN putative binding pocket and exhibited favorable interactions with the catalytic Mg(2+) ions, the nearby amino a...
Source: Bioinformation - June 15, 2013 Category: Bioinformatics Authors: Nunthaboot N, Lugsanangarm K, Kokpol S, Abd-Elazem IS Tags: Bioinformation Source Type: research