Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 11.
Bone Morphogenetic Protein-6 Promotes Cerebellar Granule Neuron Survival by Activation of the MEK/ERK/CREB Pathway.
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Monitoring Editor: Marianne Bronner-Fraser Bone morphogenetic proteins (BMPs) have been implicated in the generation and postnatal differentiation of cerebellar granule cells (CGCs). Here, we examined the eventual role of BMPs on the survival of these neurons. Lack of depolarization causes CGCs death by apoptosis in vivo, a phenomenon that is mimicked in vitro by deprivation of high potassium in cultured CGCs. We have found that BMP-6, but not BMP-7, is able to block low potassium-mediated apoptosis in CGCs. The neuroprotective effect of BMP-6 is not accompanied by an increase of Smad translocation to the nucleus, sugg...
Source: Mol Biol Cell - October 20, 2009 Category: Molecular Biology Authors: Barneda-Zahonero B, Miñano-Molina A, Badiola N, Fadó R, Xifró X, Saura CA, Rodríguez-Alvarez J Tags: Mol Biol Cell Source Type: journals
Binding the Atypical RA Domain of Ste50p to the Unfolded Opy2p Cytoplasmic Tail Is Essential for HOG Pathway.Email this article to a colleague.
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Monitoring Editor: Charles Boone Activation of the high osmolarity glycerol (HOG) pathway for osmoregulation in the yeast S. cerevisiae involves interaction of the adaptor Ste50p with the cytoplasmic tail of single-transmembrane protein Opy2p. We have determined the solution structure of the Ste50p-RA domain, and it shows an atypical RA fold lacking the beta1 and beta2 strands of the canonical motif. While the core of the RA domain is fully functional in the pheromone response, an additional region is required for the HOG pathway activation. Two peptide motifs within the intrinsically disordered cytoplasmic tail of Opy...
Source: Mol Biol Cell - October 20, 2009 Category: Molecular Biology Authors: Ekiel I, Sulea T, Jansen G, Kowalik M, Minailiuc O, Cheng J, Harcus D, Cygler M, Whiteway M, Wu C Tags: Mol Biol Cell Source Type: journals
Synthetic Lethal Genetic Interactions that Decrease Somatic Cell Proliferation in C. elegans Identify the Alternative RFCCTF18 as a Candidate Cancer Drug Target.Email this article to a colleague.
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Monitoring Editor: David G. Drubin Somatic mutations causing chromosome instability (CIN) in tumors can be exploited for selective killing of cancer cells by knockdown of second-site genes causing synthetic lethality. We tested and statistically validated synthetic lethal (SL) interactions between mutations in six S. cerevisiae CIN genes orthologous to genes mutated in colon tumors and five unlinked CIN genes. To identify which SL interactions are conserved in higher organisms and represent potential chemotherapeutic targets, we developed an assay system in Caenorhabditis elegans to test genetic interactions causing sy...
Source: Mol Biol Cell - October 20, 2009 Category: Molecular Biology Authors: McLellan J, O'Neil N, Tarailo S, Stoepel J, Bryan J, Rose A, Hieter P Tags: Mol Biol Cell Source Type: journals
Bub3p Facilitates Spindle Checkpoint Silencing in Fission Yeast.Email this article to a colleague.
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Monitoring Editor: Daniel J. Lew Although critical for spindle checkpoint signaling, the role kinetochores play in APC inhibition remains unclear. Here we show that spindle checkpoint proteins are severely depleted from unattached kinetochoresin fission yeast cells lacking Bub3p. Surprisingly, a robust mitotic arrest is maintained in the vast majority of bub3Delta cells, yet they die, suggesting that Bub3p is essential for successful checkpoint recovery. During recovery, two defects are observed: 1) cells mis-segregate chromosomes and 2) anaphase onset is significantly delayed. We show that Bub3p is required to activat...
Source: Mol Biol Cell - October 20, 2009 Category: Molecular Biology Authors: Vanoosthuyse V, Meadows JC, van der Sar SJ, Millar JB, Hardwick KG Tags: Mol Biol Cell Source Type: journals
Small Cajal Body-Specific RNAs (scaRNAs) of Drosophila Function in the Absence of Cajal Bodies.Email this article to a colleague.
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Monitoring Editor: A. Gregory Matera During their biogenesis small nuclear RNAs (snRNAs) undergo multiple covalent modifications that require guide RNAs to direct methylase and pseudouridylase enzymes to the appropriate nucleotides. Because of their localization in the nuclear Cajal body (CB), these guide RNAs are known as small CB-specific RNAs (scaRNAs). Using a fluorescent primer extension technique, we mapped the modified nucleotides in Drosophila U1, U2, U4 and U5 snRNAs. By fluorescent in situ hybridization (FISH) we showed that seven Drosophila scaRNAs are concentrated in easily detectable CBs. We used two assay...
Source: Mol Biol Cell - October 20, 2009 Category: Molecular Biology Authors: Deryusheva S, Gall JG Tags: Mol Biol Cell Source Type: journals
Polymorphisms of the lamina maturation pathway and their association with the metabolic syndrome: the DESIR prospective studyEmail this article to a colleague.
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In conclusion, we did not observe any convincing evidence that common
polymorphisms of the lamina pathway could modulate the risk of MS.
Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00109-009-0548-yAuthors
Benedicte Fontaine-Bisson, INSERM, UMR_S 937 75013 Paris FranceMarie-Christine Alessi, INSERM, UMR_S 626 13385 Marseille FranceNoemie Saut, INSERM, UMR_S 626 13385 Marseille FranceFrederic Fumeron, INSERM U695, Xavier Bichat Medical School Paris FranceMichel Marre, INSERM U695, Xavier Bichat Medical School Paris FranceAnne Dutour, INSERM, UMR_S 626 13385 Marseille FranceCatherine Badens, INSERM...
Source: Journal of Molecular Medicine - October 20, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Stem cells—meet immunityEmail this article to a colleague.
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Abstract The ability of stem cells to differentiate into various different cell types holds great promise for the treatment of irreversible
tissue damage that occurs in many debilitating conditions. With stem cell research advancing at a tremendous pace, it is becoming
clear that one of the greatest hurdles to successful stem cell-derived therapies is overcoming immune rejection of the transplant.
Although the use of immunosuppressive drugs can decrease the incidence of acute graft rejection, the burden of problems associated
with prolonged immunosuppression must be reduced. Strategies inducing specific immu...
Source: Journal of Molecular Medicine - October 20, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Mouse 3T3-L1 cells acquire resistance against oxidative stress as the adipocytes differentiate via the transcription factor FoxOEmail this article to a colleague.
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Abstract Repression of excessive increase and enlargement of adipocytes that is closely associated with obesity is effective in the
prevention and treatment of metabolic syndrome. Generally, apoptosis is induced in cells via a wide variety of intracellular
or extracellular substances, and recently, it has been suggested that the FoxO subfamily is involved in the induction of apoptosis.
We aimed to elucidate the mechanism of FoxO-mediated apoptosis-induction in the adipocytes under the reactive oxygen species
(ROS) stimulus. The treatment of differentiated and undifferentiated 3T3-L1 cells with glucose oxidas...
Source: Apoptosis - October 20, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Fzd3 and Fzd6 deficiency results in a severe midbrain morphogenesis defectEmail this article to a colleague.
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Wnt/[beta]-catenin signaling controls the proper development of the mid-/hindbrain region (MHR) and of midbrain dopaminergic (mDA) neurons, but the Frizzled (Fzd) receptors transducing these signals are still unknown. Fzd3 is expressed throughout the mouse anterior neural tube, whereas Fzd6 is restricted to the MHR. We show that the MHR is properly established and mDA neurons develop normally in Fzd6-/- mutants, but the number of mDA neurons is initially reduced and recovers at later stages in Fzd3-/- embryos. Fzd3-/-; Fzd6-/- double mutants exhibit a severe midbrain morphogenesis defect consisting of collapsed brain ventr...
Source: Developmental Dynamics - October 19, 2009 Category: Molecular Biology Authors: Sebastian Stuebner, Theresa Faus-Kessler, Thomas Fischer, Wolfgang Wurst, Nilima Prakash Source Type: journals
Syntheses of difluorinated carbasugar phosphates from trifluoroethanolEmail this article to a colleague.
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Timo Anderl, Christophe Audouard, Afjal Miah, Jonathan M. Percy, Giuseppe Rinaudo, Kuldip Singh
(Paper from Org. Biomol. Chem.)
Timo Anderl, Org. Biomol. Chem., 2009, DOI: 10.1039/b914068a
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Source: RSC - Organic Biomolecular Chemistry - October 19, 2009 Category: Molecular Biology Authors: Timo AnderlChristophe Audouard Afjal Miah Jonathan M. Percy Giuseppe Rinaudo Kuldip Singh Source Type: journals
Pyridine and pyrimidine analogs of acetaminophen as inhibitors of lipid peroxidation and cyclooxygenase and lipoxygenase catalysisEmail this article to a colleague.
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Tae-gyu Nam, Susheel J. Nara, Irene Zagol-Ikapitte, Thomas Cooper, Luca Valgimigli, John A. Oates, Ned A. Porter, Olivier Boutaud, Derek A. Pratt
(Paper from Org. Biomol. Chem.)
Tae-gyu Nam, Org. Biomol. Chem., 2009, DOI: 10.1039/b912528k
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Source: RSC - Organic Biomolecular Chemistry - October 19, 2009 Category: Molecular Biology Authors: Tae-gyu NamSusheel J. Nara Irene Zagol-Ikapitte Thomas Cooper Luca Valgimigli John A. Oates Ned A. Porter Olivier Boutaud Derek A. Pratt Source Type: journals
Introduction of disulfide bond to the main chain of PNA to switch its hybridization and invasion activityEmail this article to a colleague.
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Yuichiro Aiba, Makoto Komiyama
(Paper from Org. Biomol. Chem.)
Yuichiro Aiba, Org. Biomol. Chem., 2009, DOI: 10.1039/b917405b
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Source: RSC - Organic Biomolecular Chemistry - October 19, 2009 Category: Molecular Biology Authors: Yuichiro AibaMakoto Komiyama Source Type: journals
Characterization of unique heavy chain fibroin filaments spun underwater by the caddisfly Stenopsyche marmorata (Trichoptera; Stenopsychidae).Email this article to a colleague.
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The silks of both Lepidoptera and its sister order Trichoptera contain a homologue of heavy chain (H-fibroin), which is assumed to determine the physical properties of the fiber, such as elasticity and toughness. The long repetitive region of the H-fibroin caddisfly Stenopsyche marmorata shows a conspicuous hierarchical structure that is composed of huge units, which are mainly constructed from four large blocks (SA, SB, SC and SD) arranged in an orderly fashion. Each block contains short, distinct motifs such as SXSXSX(SX), GPXG(X)(1-3) or triplet GGX, which also occur in lepidopteran and spider filaments. The SA, SB ...
Source: Molecular Biology Reports - October 19, 2009 Category: Molecular Biology Authors: Wang Y, Sanai K, Wen H, Zhao T, Nakagaki M Tags: Mol Biol Rep Source Type: journals
Analysis of Toxoplasma gondii surface antigen 2 gene (SAG2). Relevance of genotype I in clinical toxoplasmosis.Email this article to a colleague.
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This study contributes to the scarce available information on T. gondii at South America, and reinforces an emerging concept suggesting that SAG2 type I, rather than II, parasites are a frequent cause of clinical toxoplasmosis in this continent.
PMID: 19842061 [PubMed - as supplied by publisher]
Source: Molecular Biology Reports - October 19, 2009 Category: Molecular Biology Authors: Sabaj V, Galindo M, Silva D, Sandoval L, Rodríguez JC Tags: Mol Biol Rep Source Type: journals
DNA damage and oxidative stress in human liver cell L-02 caused by surface water extracts during drinking water treatment in a waterworks in ChinaEmail this article to a colleague.
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Because of the daily and life-long exposure to disinfection by-products formed during drinking water treatment, potential adverse human health risk of drinking water disinfection is of great concern. Toxicological studies have shown that drinking water treatment increases the genotoxicity of surface water. Drinking water treatment is comprised of different potabilization steps, which greatly influence the levels of genotoxic products in the surface water and thus may alter the toxicity and genotoxicity of surface water. The aim of the present study was to understand the influence of specific steps on toxicity and genotoxic...
Source: Environmental and Molecular Mutagenesis - October 19, 2009 Category: Molecular Biology Authors: Shao-Hua Xie, Ai-Lin Liu, Yan-Yan Chen, Li Zhang, Hui-Juan Zhang, Bang-Xiong Jin, Wen-Hong Lu, Xiao-Yan Li, Wen-Qing Lu Source Type: journals
Excretion characteristics of urinary 8-hydroxydeoxyguanosine after dietary exposure to polycyclic aromatic hydrocarbonsEmail this article to a colleague.
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Urinary 8-hydroxydeoxyguanosine (8-OHdG) is considered a noninvasive marker for oxidative stress and also a marker of carcinogenic potential for compounds such as polycyclic aromatic hydrocarbons (PAHs). Although human studies have investigated urinary 8-OHdG concentrations in PAH-exposed workers and the general population, the background level and excretion kinetics of urinary 8-OHdG in humans remain unclear. Two feeding experiments (consumption of barbecued meat of 15 and 30 g/kg for Experiments 1 and 2, respectively) were conducted to examine the excretion characteristics of urinary 8-OHdG. All urine voided over 7 days ...
Source: Environmental and Molecular Mutagenesis - October 19, 2009 Category: Molecular Biology Authors: Yeh-Chung Chien, Chun-Ting Yeh Source Type: journals
Synthesis, structure and properties of decakis(phenylthio)corannuleneEmail this article to a colleague.
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Kim K. Baldridge, Kenneth I. Hardcastle, T. Jon Seiders, Jay S. Siegel
(Communication from Org. Biomol. Chem.)
Kim K. Baldridge, Org. Biomol. Chem., 2010, DOI: 10.1039/b919616a
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Source: RSC - Organic Biomolecular Chemistry - October 19, 2009 Category: Molecular Biology Authors: Kim K. BaldridgeKenneth I. Hardcastle T. Jon Seiders Jay S. Siegel Source Type: journals
The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cellsEmail this article to a colleague.
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We present a comparative study of PCH101, 236A/E7, 3G3, 206D, 150D, and 259D/C7 clones of anti-human-Foxp3 antibodies used in combination with five different fixation/permeabilization buffers. Staining for CD25, CD152, and CD127 was also compared between fixation/permeabilization treatments. Promising antibody/buffer combinations were tested in a panel of peripheral blood mononuclear cells from 10 individuals, and then on fresh versus frozen cells from four individuals. Finally, different fluorochromes coupled to two representative antibodies were compared to optimize separation of Foxp3+ from Foxp3- events. Foxp3 gates we...
Source: Cytometry Part A - October 19, 2009 Category: Molecular Biology Authors: Jacqueline P. Law, Dale F. Hirschkorn, Rachel E. Owen, Hope H. Biswas, Philip J. Norris, Marion C. Lanteri Source Type: journals
Image segmentation and dynamic lineage analysis in single-cell fluorescence microscopyEmail this article to a colleague.
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An increasingly common component of studies in synthetic and systems biology is analysis of dynamics of gene expression at the single-cell level, a context that is heavily dependent on the use of time-lapse movies. Extracting quantitative data on the single-cell temporal dynamics from such movies remains a major challenge. Here, we describe novel methods for automating key steps in the analysis of single-cell, fluorescent images - segmentation and lineage reconstruction - to recognize and track individual cells over time. The automated analysis iteratively combines a set of extended morphological methods for segmentation, ...
Source: Cytometry Part A - October 19, 2009 Category: Molecular Biology Authors: Quanli Wang, Jarad Niemi, Chee-Meng Tan, Lingchong You, Mike West Source Type: journals
Proteomic analysis reveals overlapping functions of clustered protocadherins.Email this article to a colleague.
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The three tandem-arrayed protocadherin (Pcdh) gene clusters, namely Pcdh-a, Pcdh-ss and Pcdh-, play important roles in the development of the vertebrate central nervous system. To gain insight into the molecular action of Pcdhs, we performed a systematic proteomic analysis of Pcdh--associated protein complexes. We identified a list of 154 non-redundant proteins in the Pcdh- complexes. This list includes nearly 30 members of clustered Pcdh -a, -ss and - families as core components of the complexes and additional over 120 putative Pcdh associated proteins. We validated a selected subset of Pcdh--associated proteins using...
Source: Molecular and Cellular Proteomics : MCP - October 19, 2009 Category: Molecular Biology Authors: Han MH, Lin C, Meng S, Wang X Tags: Mol Cell Proteomics Source Type: journals
An automated and multiplexed method for high throughput peptide immunoaffinity enrichment and multiple reaction monitoring mass spectrometry-based quantification of protein biomarkers.Email this article to a colleague.
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There is an urgent need for quantitative assays in verifying and validating the large numbers of protein biomarker candidates produced in modern "-omics" experiments. SISCAPA (Stable Isotope Standards with Capture by Anti-Peptide Antibodies) has shown tremendous potential to meet this need by combining peptide immunoaffinity enrichment with quantitative mass spectrometry. In this paper, we describe three significant advances to the SISCAPA technique. First, we develop a method for an automated magnetic bead-based platform capable of high throughput processing. Second, we implement the automated method in a multiplexed ...
Source: Molecular and Cellular Proteomics : MCP - October 19, 2009 Category: Molecular Biology Authors: Whiteaker JR, Zhao L, Anderson L, Paulovich AG Tags: Mol Cell Proteomics Source Type: journals
Metabolic profiling reveals key metabolic features of renal cell carcinoma.Email this article to a colleague.
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Abstract Recent evidence suggests that metabolic changes play a pivotal role in the biology of cancer and in particular renal cell carcinoma (RCC). Here, a global metabolite profiling approach was applied to characterize the metabolite pool of RCC and normal renal tissue. Advanced decision tree models were applied to characterize the metabolic signature of RCC and to explore features of metastasized tumors. The findings were validated in a second independent dataset. Vitamin E derivates and metabolites of glucose, fatty acid, and inositol phosphate metabolism determined the metabolic profile of RCC. Alpha-tocopherol, h...
Source: J Cell Mol Med - October 19, 2009 Category: Molecular Biology Authors: Catchpole G, Platzer A, Weikert C, Kempkensteffen C, Johannsen M, Krause H, Jung K, Miller K, Willmitzer L, Selbig J, Weikert S Tags: J Cell Mol Med Source Type: journals
Expression of TRPC6 Channels in Human Lung Macrophages.Email this article to a colleague.
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This study examined the expression and function of transient receptor potential (TRP) channels in human macrophages. Human alveolar macrophages and lung tissue macrophages expressed increased mRNA and protein for TRPC6 when compared with monocytes and monocyte-derived-macrophages. Moreover, TRPC6 mRNA expression was significantly elevated in alveolar macrophages from COPD patients compared to control subjects. There were no differences in mRNA for TRPC3 or TRPC7. Although mRNA for TRPM2 and TRPV1 was detected in these cells, protein expression could not be determined. Fractionation of lung derived-macrophages demonstrated ...
Source: American Journal of Respiratory Cell and Molecular Biology - October 19, 2009 Category: Molecular Biology Authors: Finney-Hayward TK, Popa O, Bahra P, Li S, Poll CT, Gosling M, Nicholson AG, Russell RE, Kon OM, Jarai G, Westwick J, Barnes PJ, Donnelly LE Tags: Am J Respir Cell Mol Biol Source Type: journals
MMP-19 Deficiency Promotes Tenascin-C Accumulation and Allergen-induced Airway Inflammation.Email this article to a colleague.
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Matrix metalloproteinases (MMPs) recently appeared as key regulators of inflammation, allowing recruitment and clearance of inflammatory cells and modifying the biological activity of many peptidic mediators by cleavage. MMP-19 is a newly described MMP and preferentially cleaves matrix proteins such as collagens and tenascin-C. The role of MMP-19 in asthma has not been described to date. The purpose of the present study was to assess MMP-19 expression in a murine asthma model and to address biological effects of MMP-19 deficiency in mice. Allergen-exposed wild-type (WT) mice displayed an increased expression of MMP-19 ...
Source: American Journal of Respiratory Cell and Molecular Biology - October 19, 2009 Category: Molecular Biology Authors: Gueders MM, Hirst SJ, Quesada-Calvo F, Paulissen G, Hacha J, Gilles C, Gosset P, Louis R, Foidart JM, Lopez-Otin C, Noël A, Cataldo DD Tags: Am J Respir Cell Mol Biol Source Type: journals
Acute Lung Injury but not Sepsis is Associated with Increased Colony Formation by Peripheral Blood Mononuclear Cells.Email this article to a colleague.
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Acute lung injury (ALI) and severe sepsis are common critical illnesses associated with mobilization of bone marrow-derived cells into circulation. By identifying and determining these cellsâ functional characteristics, unique prognostic biomarkers can be developed to help investigators understand mechanisms underlying the pathophysiology of these disorders. We previously demonstrated increased colony forming unit (CFU) ability of circulating peripheral blood mononuclear cells (PBMCs) from patients with ALI compared to healthy controls that also correlated with improved survival. Here we hypothesized that increase...
Source: American Journal of Respiratory Cell and Molecular Biology - October 19, 2009 Category: Molecular Biology Authors: Burnham EL, Mealer M, Gaydos J, Majka S, Moss M Tags: Am J Respir Cell Mol Biol Source Type: journals
Protein Thiol Oxidation in Murine Airway Epithelial Cells in Response to Naphthalene or Diethyl Maleate.Email this article to a colleague.
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Naphthalene is a semi-volatile aromatic hydrocarbon to which humans are exposed from a variety of sources. Naphthalene results in acute cytotoxicity to respiratory epithelium in rodents. Cytochrome P450-dependent metabolic activation to form reactive intermediates and loss of soluble cellular thiols (glutathione) are critical steps in naphthalene toxicity, but the precise mechanisms by which this chemical results in cellular injury remain unclear. Protein thiols are likely targets of reactive naphthalene metabolites. Loss of these, either through adduction or thiol oxidation mechanisms, may be important underlying mech...
Source: American Journal of Respiratory Cell and Molecular Biology - October 19, 2009 Category: Molecular Biology Authors: Spiess PC, Morin D, Williams CR, Buckpitt AR Tags: Am J Respir Cell Mol Biol Source Type: journals
TSLP Induces Chemotactic and Pro-survival Effects in Eosinophils: Implications in Allergic Inflammation.Email this article to a colleague.
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Thymic stromal lymphopoietin (TSLP) is highly expressed by bronchial epithelial cells and skin keratinocytes in allergic diseases. TSLP acts as a master switch for allergic inflammation through the activation of dendritic cells and mast cells for initiating inflammatory type 2 T-helper lymphocyte (Th2) responses. To elucidate the immunological cascades of epithelium/keratinocyte-eosinophil mediated allergic inflammation, we examined the modulating effects of TSLP on human eosinophils. Expression of TSLP receptor complex was detected by RT-PCR, flow cytometry and Western blot. Adhesion molecules, cytokine and chemokines...
Source: American Journal of Respiratory Cell and Molecular Biology - October 19, 2009 Category: Molecular Biology Authors: Wong CK, Hu S, Cheung PF, Lam CW Tags: Am J Respir Cell Mol Biol Source Type: journals
Temperature and salt concentration alter base-sequence selectivity of a duplex DNA-binding proteinEmail this article to a colleague.
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Satoru Nagatoishi, Yoshikazu Tanaka, Motonori Kudou, Kouhei Tsumoto
(Communication from Mol. BioSyst.)
Satoru Nagatoishi, Mol. BioSyst., 2010, DOI: 10.1039/b914828k
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Source: RSC - Mol. BioSyst. latest articles - October 18, 2009 Category: Molecular Biology Authors: Satoru NagatoishiYoshikazu Tanaka Motonori Kudou Kouhei Tsumoto Source Type: journals
Complex genetic regulation of protein glycosylationEmail this article to a colleague.
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Gordan Lauc, Igor Rudan, Harry Campbell, Pauline M. Rudd
(Review from Mol. BioSyst.)
Gordan Lauc, Mol. BioSyst., 2010, DOI: 10.1039/b910377e
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Source: RSC - Mol. BioSyst. latest articles - October 18, 2009 Category: Molecular Biology Authors: Gordan LaucIgor Rudan Harry Campbell Pauline M. Rudd Source Type: journals
Detection of single nucleotide polymorphisms using a DNA Holliday junction nanoswitch-a high-throughput fluorescence lifetime assayEmail this article to a colleague.
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Colin D. McGuinness, Mira K. Y. Nishimura, David Keszenman-Pereyra, Paul Dickinson, Colin J. Campbell, Till T. Bachmann, Peter Ghazal, Jason Crain
(Paper from Mol. BioSyst.)
Colin D. McGuinness, Mol. BioSyst., 2010, DOI: 10.1039/b913455g
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Source: RSC - Mol. BioSyst. latest articles - October 18, 2009 Category: Molecular Biology Authors: Colin D. McGuinnessMira K. Y. Nishimura David Keszenman-Pereyra Paul Dickinson Colin J. Campbell Till T. Bachmann Peter Ghazal Jason Crain Source Type: journals
SecB-A chaperone dedicated to protein translocationEmail this article to a colleague.
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Philipp Bechtluft, Nico Nouwen, Sander J. Tans, Arnold J. M. Driessen
(Review from Mol. BioSyst.)
Philipp Bechtluft, Mol. BioSyst., 2010, DOI: 10.1039/b915435c
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Source: RSC - Mol. BioSyst. latest articles - October 18, 2009 Category: Molecular Biology Authors: Philipp BechtluftNico Nouwen Sander J. Tans Arnold J. M. Driessen Source Type: journals
Recent advances and novel approaches in deriving neurons from stem cellsEmail this article to a colleague.
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Steven J. Greco, Pranela Rameshwar
(Review from Mol. BioSyst.)
Steven J. Greco, Mol. BioSyst., 2010, DOI: 10.1039/b914822c
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Source: RSC - Mol. BioSyst. latest articles - October 18, 2009 Category: Molecular Biology Authors: Steven J. GrecoPranela Rameshwar Source Type: journals
Macrocyclic aromatic polysulfones and sulfide-sulfones: synthesis and structural characterisation of molecular pentagons and rectanglesEmail this article to a colleague.
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Abderrazak Ben-Haida, Howard M. Colquhoun, Philip Hodge, James Raftery, Andrew J. P. White, David J. Williams
(Paper from Org. Biomol. Chem.)
Abderrazak Ben-Haida, Org. Biomol. Chem., 2009, DOI: 10.1039/b912193e
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Source: RSC - Organic Biomolecular Chemistry - October 18, 2009 Category: Molecular Biology Authors: Abderrazak Ben-HaidaHoward M. Colquhoun Philip Hodge James Raftery Andrew J. P. White David J. Williams Source Type: journals
Chemical modification of biogenous iron oxide to create an excellent enzyme scaffoldEmail this article to a colleague.
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Takashi Sakai, Yuki Miyazaki, Ai Murakami, Noriko Sakamoto, Tadashi Ema, Hideki Hashimoto, Mitsuaki Furutani, Makoto Nakanishi, Tatsuo Fujii, Jun Takada
(Communication from Org. Biomol. Chem.)
Takashi Sakai, Org. Biomol. Chem., 2010, DOI: 10.1039/b919497e
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Source: RSC - Organic Biomolecular Chemistry - October 18, 2009 Category: Molecular Biology Authors: Takashi SakaiYuki Miyazaki Ai Murakami Noriko Sakamoto Tadashi Ema Hideki Hashimoto Mitsuaki Furutani Makoto Nakanishi Tatsuo Fujii Jun Takada Source Type: journals
Towards an asymmetric synthesis of the bacterial peptide deformylase (PDF) inhibitor fumimycinEmail this article to a colleague.
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Caroline E. Hartmann, Patrick J. Gross, Martin Nieger, Stefan Brase
(Communication from Org. Biomol. Chem.)
Caroline E. Hartmann, Org. Biomol. Chem., 2009, DOI: 10.1039/b916372g
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Source: RSC - Organic Biomolecular Chemistry - October 18, 2009 Category: Molecular Biology Authors: Caroline E. HartmannPatrick J. Gross Martin Nieger Stefan Brase Source Type: journals
Sensitive and specific enzyme immunoassays for antigenic trisaccharide from Bacillus anthracis sporesEmail this article to a colleague.
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Sandrine G. Y. Dhenin, Vincent Moreau, Marie-Claire Nevers, Christophe Creminon, Florence Djedaini-Pilard
(Paper from Org. Biomol. Chem.)
Sandrine G. Y. Dhenin, Org. Biomol. Chem., 2009, DOI: 10.1039/b914534f
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The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 18, 2009 Category: Molecular Biology Authors: Sandrine G. Y. DheninVincent Moreau Marie-Claire Nevers Christophe Creminon Florence Djedaini-Pilard Source Type: journals
Expansion of human mesenchymal stromal cells on microcarriers: growth and metabolismEmail this article to a colleague.
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This study selected a suitable microcarrier for human bone marrow-derived stromal cells (HBMSCs), optimized cell-seeding strategies by varying serum concentrations, and optimized dynamic expansion of the HBMSCs in a microcarrier-based spinner flask cultivation system by applying various feeding regimes. Cytodex 1 microcarriers in combination with a low-serum concentration (0-5%) in the medium resulted in the highest seeding efficiency for the HBMSCs. Subsequently, significant expansion of the HBMSCs on these carriers has been observed. The highest number of HBMSCs population doublings (4.8 doublings) was obtained by a comb...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: D. Schop, R. van Dijkhuizen-Radersma, E. Borgart, F. W. Janssen, H. Rozemuller, H.-J. Prins, J. D. de Bruijn Source Type: journals
Compressed collagen gel: a novel scaffold for human bladder cellsEmail this article to a colleague.
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Collagen is highly conserved across species and has been used extensively for tissue regeneration; however, its mechanical properties are limited. A recent advance using plastic compression of collagen gels to achieve much higher concentrations significantly increases its mechanical properties at the neo-tissue level. This controlled, cell-independent process allows the engineering of biomimetic scaffolds. We have evaluated plastic compressed collagen scaffolds seeded with human bladder smooth muscle cells inside and urothelial cells on the gel surface for potential urological applications. Bladder smooth muscle and urothe...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: E.-M. Engelhardt, E. Stegberg, R. A. Brown, J. A. Hubbell, F. M. Wurm, M. Adam, P. Frey Source Type: journals
Donor-matched comparison of dental pulp stem cells and bone marrow-derived mesenchymal stem cells in a rat modelEmail this article to a colleague.
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Dental pulp stem cells (DPSCs) have drawn much interest for the regeneration of mineralized tissues, and several studies have compared DPSCs to bone marrow-derived mesenchymal stem cells (BMMSCs). However, conflicting results, possibly due to donor-associated variability, have been published and the regenerative potential of DPSCs is currently unclear. In the present study we have sought to address this problem using a donor-matched experimental design to robustly compare the biological properties of DPSCs and BMMSCs. All experiments were performed using cells isolated from a single adult Sprague-Dawley rat. Our results sh...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: Daniel L. Alge, Dan Zhou, Lyndsey L. Adams, Brandon K. Wyss, Matthew D. Shadday, Erik J. Woods, T. M. Gabriel Chu, W. Scott Goebel Source Type: journals
Vibration stimulates vocal mucosa-like matrix expression by hydrogel-encapsulated fibroblastsEmail this article to a colleague.
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The objective of this study was to investigate the effect of physiologically relevant vibratory stimulation on ECM gene expression and synthesis by fibroblasts encapsulated within hyaluronic acid hydrogels that approximate the viscoelastic properties of vocal mucosa. Relative to static controls, samples exposed to vibration exhibited significant increases in mRNA expression levels of HA synthase 2, decorin, fibromodulin and MMP-1, while collagen and elastin expression were relatively unchanged. Expression levels exhibited a temporal response, with maximum increases observed after 3 and 5 days of vibratory stimulation and s...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: Jaishankar K. Kutty, Ken Webb Source Type: journals
Human cell culture process capability: a comparison of manual and automated productionEmail this article to a colleague.
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Cell culture is one of the critical bioprocessing steps required to generate sufficient human-derived cellular material for most cell-based therapeutic applications in regenerative medicine. Automated cell expansion is fundamental to the development of scaled, robust and cost effective commercial production processes for cell-based therapeutic products. This paper describes the first application of process capability analysis to establish and compare the short-term process capability of manual and automated processes for the in vitro expansion of a selected anchorage-dependent cell line. Estimates of the process capability...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: Yang Liu, Paul Hourd, Amit Chandra, David J. Williams Source Type: journals
A tissue-like construct of human bone marrow MSCs composite scaffold support in vivo ectopic bone formationEmail this article to a colleague.
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This study reports on a new cell-scaffold construct composed of gelatin-based hydrogel and ceramic (CaCO3/[beta]-TCP) particles loaded with human MSCs producing a tissue-like construct applied as a transplant for in vivo bone formation. Bone marrow-derived human MSCs were cultured in osteogenic induction medium. 5 × 105 (P2) cells were loaded on a mixture of hydrogel microspheres and ceramic particles, cultured in a rotating dynamic culture for up to 3 weeks. Both hydrogel microspheres and ceramic particles coalesced together to form a tissue-like construct, shown by histology to contain elongated spindle-like cells formi...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: D. Ben-David, T. Kizhner, E. Livne, S. Srouji Source Type: journals
Effects of mechanical loading on collagen propeptides processing in cartilage repairEmail this article to a colleague.
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Injured articular cartilage has poor reparative capabilities and if left untreated may develop into osteoarthritis. Unsatisfactory results with conventional treatment methods have brought as an alternative treatment the development of matrix autologous chondrocyte transplants (MACTs). Recent evidence proposes that the maintenance of the original phenotype by isolated chondrocytes grown in a scaffold transplant is linked to mechanical compression, because macromolecules, particularly collagen, of the extracellular matrix have the ability to 'self-assemble'. In load-bearing tissues, collagen is abundantly present and mechani...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: Rosmarie Hardmeier, Heinz Redl, Stefan Marlovits Source Type: journals
Breaking the hierarchy - a new cluster selection mechanism for hierarchical clustering methodsEmail this article to a colleague.
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Conclusions:
Using our new cluster selection method together with the method by Palla et al. provides a new interesting clustering mechanism that allows to compute overlapping clusters which is especially valuable for biological and chemical data sets.
Source: Algorithms for Molecular Biology - October 18, 2009 Category: Molecular Biology Authors: Laszlo ZahoranszkyGyula KatonaPeter HariAndras Malnasi-CsizmadiaKatharina ZweigGergely Zahoranszky-Kohalmi Source Type: journals
Xenopus axin-related protein: A link between its centrosomal localization and function in the Wnt/[beta]-catenin pathwayEmail this article to a colleague.
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The Wnt/[beta]-catenin signaling pathway regulates cell proliferation and cell fate determination in multiple systems. However, the subcellular localization of Wnt pathway components and the significance of this localization for the pathway regulation have not been extensively analyzed. Here we report that Xenopus Axin-related protein (XARP), a component of the [beta]-catenin destruction complex, is localized to the centrosome. This localization of XARP requires the presence of the DIX domain and an adjacent region. Since other components of the Wnt pathway have also been shown to associate with the centrosome, we tested a...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Evguenia M. Alexandrova, Sergei Y. Sokol Source Type: journals
Identification of novel genes involved in the development of the sword and gonopodium in swordtail fishEmail this article to a colleague.
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COVER PHOTOGRAPH: Bony fin rays of the gonopodium, an intromittent organ, of the male swordtail fish, Xiphophorus helleri. Distal skeletal elements are differentiated into hook-like structures that facilitate efficient sperm transfer during internal fertilization. From Offen et al., Developmental Dynamics 238:1674-1687, 2009. © 2009 Wiley-Liss, Inc.
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Nils Offen, Axel Meyer, Gerrit Begemann Source Type: journals
Highlights in DDEmail this article to a colleague.
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No abstract.
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Julie C. Kiefer Source Type: journals
DD ArtPixEmail this article to a colleague.
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No abstract.
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Source Type: journals
Foxg1 is required for proper separation and formation of sensory cristae during inner ear developmentEmail this article to a colleague.
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The vestibular portion of the inner ear, the three semicircular canals and their sensory cristae, is responsible for detecting angular head movements. It was proposed that sensory cristae induce formation of their non-sensory components, the semicircular canals. Here, we analyzed the inner ears of Foxg1-/- mouse mutants, which display vestibular defects that are in conflict with the above model. In Foxg1-/- ears, the lateral canal is present without the lateral ampulla, which houses the lateral crista. Our gene expression analyses indicate that at the time when canal specification is thought to occur, the prospective later...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Chan Ho Hwang, Antonio Simeone, Eseng Lai, Doris K. Wu Source Type: journals
Role of VEGF and tissue hypoxia in patterning of neural and vascular cells recruited to the embryonic heartEmail this article to a colleague.
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We hypothesized that oxygen gradients and hypoxia-responsive signaling may play a role in the patterning of neural or vascular cells recruited to the developing heart. Endothelial progenitor and neural cells are recruited to and form branched structures adjacent to the relatively hypoxic outflow tract (OFT) myocardium from stages 27-32 (ED6.5-7.5) of chick development. As determined by whole mount confocal microscopy, the neural and vascular structures were not anatomically associated. Adenoviral delivery of a VEGF trap dramatically affected the remodeling of the vascular plexus into a coronary tree while neuronal branchin...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Hongbin Liu, Qiwei Yang, Krishnan Radhakrishnan, Dedra E. Whitfield, Camille L. M. Everhart, Patricia Parsons-Wingerter, Steven A. Fisher Source Type: journals
