Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 12.
Human cell culture process capability: a comparison of manual and automated production
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Cell culture is one of the critical bioprocessing steps required to generate sufficient human-derived cellular material for most cell-based therapeutic applications in regenerative medicine. Automated cell expansion is fundamental to the development of scaled, robust and cost effective commercial production processes for cell-based therapeutic products. This paper describes the first application of process capability analysis to establish and compare the short-term process capability of manual and automated processes for the in vitro expansion of a selected anchorage-dependent cell line. Estimates of the process capability...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: Yang Liu, Paul Hourd, Amit Chandra, David J. Williams Source Type: journals
A tissue-like construct of human bone marrow MSCs composite scaffold support in vivo ectopic bone formationEmail this article to a colleague.
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This study reports on a new cell-scaffold construct composed of gelatin-based hydrogel and ceramic (CaCO3/[beta]-TCP) particles loaded with human MSCs producing a tissue-like construct applied as a transplant for in vivo bone formation. Bone marrow-derived human MSCs were cultured in osteogenic induction medium. 5 × 105 (P2) cells were loaded on a mixture of hydrogel microspheres and ceramic particles, cultured in a rotating dynamic culture for up to 3 weeks. Both hydrogel microspheres and ceramic particles coalesced together to form a tissue-like construct, shown by histology to contain elongated spindle-like cells formi...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: D. Ben-David, T. Kizhner, E. Livne, S. Srouji Source Type: journals
Effects of mechanical loading on collagen propeptides processing in cartilage repairEmail this article to a colleague.
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Injured articular cartilage has poor reparative capabilities and if left untreated may develop into osteoarthritis. Unsatisfactory results with conventional treatment methods have brought as an alternative treatment the development of matrix autologous chondrocyte transplants (MACTs). Recent evidence proposes that the maintenance of the original phenotype by isolated chondrocytes grown in a scaffold transplant is linked to mechanical compression, because macromolecules, particularly collagen, of the extracellular matrix have the ability to 'self-assemble'. In load-bearing tissues, collagen is abundantly present and mechani...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 18, 2009 Category: Molecular Biology Authors: Rosmarie Hardmeier, Heinz Redl, Stefan Marlovits Source Type: journals
Breaking the hierarchy - a new cluster selection mechanism for hierarchical clustering methodsEmail this article to a colleague.
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Conclusions:
Using our new cluster selection method together with the method by Palla et al. provides a new interesting clustering mechanism that allows to compute overlapping clusters which is especially valuable for biological and chemical data sets.
Source: Algorithms for Molecular Biology - October 18, 2009 Category: Molecular Biology Authors: Laszlo ZahoranszkyGyula KatonaPeter HariAndras Malnasi-CsizmadiaKatharina ZweigGergely Zahoranszky-Kohalmi Source Type: journals
Xenopus axin-related protein: A link between its centrosomal localization and function in the Wnt/[beta]-catenin pathwayEmail this article to a colleague.
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The Wnt/[beta]-catenin signaling pathway regulates cell proliferation and cell fate determination in multiple systems. However, the subcellular localization of Wnt pathway components and the significance of this localization for the pathway regulation have not been extensively analyzed. Here we report that Xenopus Axin-related protein (XARP), a component of the [beta]-catenin destruction complex, is localized to the centrosome. This localization of XARP requires the presence of the DIX domain and an adjacent region. Since other components of the Wnt pathway have also been shown to associate with the centrosome, we tested a...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Evguenia M. Alexandrova, Sergei Y. Sokol Source Type: journals
Identification of novel genes involved in the development of the sword and gonopodium in swordtail fishEmail this article to a colleague.
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COVER PHOTOGRAPH: Bony fin rays of the gonopodium, an intromittent organ, of the male swordtail fish, Xiphophorus helleri. Distal skeletal elements are differentiated into hook-like structures that facilitate efficient sperm transfer during internal fertilization. From Offen et al., Developmental Dynamics 238:1674-1687, 2009. © 2009 Wiley-Liss, Inc.
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Nils Offen, Axel Meyer, Gerrit Begemann Source Type: journals
Highlights in DDEmail this article to a colleague.
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No abstract.
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Julie C. Kiefer Source Type: journals
DD ArtPixEmail this article to a colleague.
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No abstract.
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Source Type: journals
Foxg1 is required for proper separation and formation of sensory cristae during inner ear developmentEmail this article to a colleague.
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The vestibular portion of the inner ear, the three semicircular canals and their sensory cristae, is responsible for detecting angular head movements. It was proposed that sensory cristae induce formation of their non-sensory components, the semicircular canals. Here, we analyzed the inner ears of Foxg1-/- mouse mutants, which display vestibular defects that are in conflict with the above model. In Foxg1-/- ears, the lateral canal is present without the lateral ampulla, which houses the lateral crista. Our gene expression analyses indicate that at the time when canal specification is thought to occur, the prospective later...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Chan Ho Hwang, Antonio Simeone, Eseng Lai, Doris K. Wu Source Type: journals
Role of VEGF and tissue hypoxia in patterning of neural and vascular cells recruited to the embryonic heartEmail this article to a colleague.
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We hypothesized that oxygen gradients and hypoxia-responsive signaling may play a role in the patterning of neural or vascular cells recruited to the developing heart. Endothelial progenitor and neural cells are recruited to and form branched structures adjacent to the relatively hypoxic outflow tract (OFT) myocardium from stages 27-32 (ED6.5-7.5) of chick development. As determined by whole mount confocal microscopy, the neural and vascular structures were not anatomically associated. Adenoviral delivery of a VEGF trap dramatically affected the remodeling of the vascular plexus into a coronary tree while neuronal branchin...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Hongbin Liu, Qiwei Yang, Krishnan Radhakrishnan, Dedra E. Whitfield, Camille L. M. Everhart, Patricia Parsons-Wingerter, Steven A. Fisher Source Type: journals
Embryogenesis and ossification of Emydura subglobosa (Testudines, Pleurodira, Chelidae) and patterns of turtle developmentEmail this article to a colleague.
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Using the Standard Event System (SES) to study patterns of vertebrate development, we describe a series of 17 embryos of the pleurodire turtle Emydura subglobosa. Based on a sequence heterochrony analysis including 23 tetrapod taxa, we identified autapomorphic developmental shifts that characterise Testudines, Cryptodira, and Pleurodira. The main results are that Testudines are characterised by an autapomorphic late neck development, whereas pleurodires and cryptodires show a different developmental timing of the mandibular process. Additionally, we described the ossification pattern of E. subglobosa and compared the data ...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Ingmar Werneburg, Jasmina Hugi, Johannes Müller, Marcelo R. Sánchez-Villagra Source Type: journals
Functional characterization of cardiac progenitor cells and their derivatives in the embryonic heart post-chamber formationEmail this article to a colleague.
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There is scant information on the fate of cardiac progenitor cells (CPC) in the embryonic heart after chamber specification. Here we simultaneously tracked three lineage-specific markers (Nkx2.5, MLC2v, and ANF) and confirmed that CPCs with an Nkx2.5+MLC2v-ANF- phenotype are present in the embryonic (E) day 11.5 mouse ventricular myocardium. We demonstrated that these CPCs could give rise to working cardiomyocytes and conduction system cells. Using a two-photon imaging analysis, we found that the majority of CPCs are not capable of developing Ca2+ transients in response to [beta]-adrenergic receptor stimulation. In contras...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Nichole M. McMullen, Feixiong Zhang, Adam Hotchkiss, Frederic Bretzner, Jennifer M. Wilson, Hong Ma, Karim Wafa, Robert M. Brownstone, Kishore B.S. Pasumarthi Source Type: journals
Mechanisms for split localization of Fgf10 expression in early lung developmentEmail this article to a colleague.
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In early lung development, epithelial tubes (lung buds) intrude into mesenchyme covered with pleural cells (lung border), and form tree-like networks, by means of repeated use of morphogenetic processes: "elongation," "terminal bifurcation," and "lateral budding." When a bud is elongating, a peak of Fgf10 expression is formed in the mesenchyme near the tip; whereas when terminal bifurcation and lateral budding occur, two separate peaks are formed instead. To explain the spatial pattern of Fgf10 expression, we developed a mathematical model for the regulation of Fgf10 expression with geometrical conditions including shapes ...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Tsuyoshi Hirashima, Yoh Iwasa, Yoshihiro Morishita Source Type: journals
Progenitor cell maturation in the developing vertebrate retinaEmail this article to a colleague.
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Progenitor cells in the developing retina initially divide so that each division produces two cells that divide again. Subsequently, progenitor cells change their mode of division so that one or both cells produced by a division can withdraw from the mitotic cycle and differentiate. We asked how these two progenitor cell stages differ molecularly and what controls the switch in the mode of division. We show that early preneurogenic progenitor cells express the transcription factor, Sox2, and the Notch ligand, Delta1. More mature neurogenic progenitor cells express Sox2 and the bHLH transcription factor, E2A, and not Delta1...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Hyun-Jin Yang, Amila O. Silva, Naoko Koyano-Nakagawa, Steven C. McLoon Source Type: journals
A developmental study of enteric neuron migration in the grasshopper using immunological probesEmail this article to a colleague.
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Motility of enteric plexus neurons in the grasshopper Locusta migratoria depends critically on the NO/cGMP signaling cascade. This is reflected in a strong NO-dependent cGMP staining in migrating enteric midgut neurons. In contrast, first cGMP immunoreactivity (cGMP-IR) in the foregut enteric ganglia was detected clearly after the main migratory processes have taken place. Thus, expression of cGMP-IR in migrating neurons is a distinct phenomenon restricted to neurons forming midgut and foregut plexus, that does not occur during convergence of neurons forming the enteric ganglia. Analysis of time lapse video microscopy of m...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Sabine Knipp, Gerd Bicker Source Type: journals
Comparative expression of zebrafish lats1 and lats2 and their implication in gastrulation movementsEmail this article to a colleague.
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Large tumor suppressor (Lats) is a Ser/Thr kinase, and it presents an important function in tumor suppression. lats was originally identified in Drosophila and recently in mammals. In mammals, it contains two homologues, lats1 and lats2. In the present study, lats1 and lats2 were characterized from zebrafish (Danio rerio), which is the first report of lats in a nonmammalian vertebrate. The primary structure, genomic organization, and phylogenesis of lats from different species were studied, and the results suggest that lats1 is the direct descendant of invertebrate lats, whereas lats2 is formed by genome duplication. In ze...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Chun-Hong Chen, Yong-Hua Sun, De-Sheng Pei, Zuo-Yan Zhu Source Type: journals
Developmental expression of Smoc1 and Smoc2 suggests potential roles in fetal gonad and reproductive tract differentiationEmail this article to a colleague.
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We examined the expression and regulation of Smoc1 and Smoc2 in fetal gonad/mesonephros complexes to discover possible roles for these genes in gonad and mesonephros development. Smoc1 was upregulated at [sim]E10.75 in a center-to-poles wave in pre-Sertoli and pre-granulosa cells and its expression was greatly reduced in Wt1, Sf1, and Fog2 mutants. After E13.5, Smoc1 was downregulated in an anterior-to-posterior wave in granulosa cells but persisted in Sertoli cells, suggesting a sexually dimorphic requirement in supporting cell lineage differentiation. Smoc2 was expressed in Leydig cells, mesonephroi, and Wnt4 mutant ovar...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Dorothy E. Pazin, Kenneth H. Albrecht Source Type: journals
Homozygous disruption of the tip60 gene causes early embryonic lethalityEmail this article to a colleague.
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Tat-interactive protein 60 (Tip60) is a member of the MYST family, proteins of which are related by an atypical histone acetyltransferase (HAT) domain. Although Tip60 has been implicated in cellular activities including DNA repair, apoptosis, and transcriptional regulation, its function during embryonic development is unknown. We ablated the Tip60 gene (Htatip) from the mouse by replacing exons 1-9 with a neomycin resistance cassette. Development and reproduction of wild-type and heterozygous animals were normal. However, homozygous ablation of the Tip60 gene caused embryolethality near the blastocyst stage of development,...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Yaofei Hu, Joseph B. Fisher, Stacy Koprowski, Donna McAllister, Min-Su Kim, John Lough Source Type: journals
Osteoblast maturation occurs in overlapping proximal-distal compartments during fin regeneration in zebrafishEmail this article to a colleague.
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During fin regeneration, osteoblasts must continually differentiate for outgrowth of the bony fin rays. Bone maturity increases in a distal-proximal manner, and osteoblast maturation can be detected similarly when following gene expression. We find that early markers for osteoblast differentiation are expressed in a discrete domain at the distal end of the fin, just proximal to the adjacent germinal compartment of dividing cells. Matrix genes, required at later stages developmentally, are expressed in a population of cells proximally to the early genes. A marker for mature osteoblasts is expressed in cells further proximal...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Andrew M. Brown, Shannon Fisher, M. Kathryn Iovine Source Type: journals
Chromosomal position mediates spinal cord expression of a dbx1a enhancerEmail this article to a colleague.
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Dbx homeodomain proteins are important for the production of multiple spinal cord cell types. To examine the regulation of Dbx genes in more detail, we have generated transgenic zebrafish in which fluorescent protein expression is driven by predicted dbx1a enhancers. We identified three areas of sequence conservation upstream of the dbx1a coding sequence and generated fluorescent reporter constructs driven by these predicted enhancer elements and the endogenous dbx1a promoter. In multiple stable insertions of a 3.5-kb enhancer fragment, we observed that there was additional reporter expression in the dorsal spinal cord not...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Suzanna L. Gribble, O. Brant Nikolaus, M. Scott Carver, Kazuyuki Hoshijima, Richard I. Dorsky Source Type: journals
Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebratesEmail this article to a colleague.
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We describe the temporal and spatial expression patterns of these genes through embryonic development. All zebrafish actinin genes have unique expression profiles, indicating specialization of each gene. In particular, the muscle actinins display preferential expression in different domains of axial, pharyngeal, and cranial musculature. There is no identified avian actn3 and approximately 16% of humans are null for ACTN3. Duplication of actn3 in the zebrafish indicates that variation in actn3 expression may promote physiological diversity in muscle function among vertebrates. Developmental Dynamics 238:2936-2947, 2009. © ...
Source: Developmental Dynamics - October 18, 2009 Category: Molecular Biology Authors: Christopher K. Holterhoff, Rebecca H. Saunders, Erika E. Brito, Daniel S. Wagner Source Type: journals
Breaking the hierarchy - a new cluster selection mechanism for hierarchical clustering methods.Email this article to a colleague.
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CONCLUSIONS: Using our new cluster selection method together with the method by Palla et al. provides a new interesting clustering mechanism that allows to compute overlapping clusters which is especially valuable for biological and chemical data sets.
PMID: 19840391 [PubMed - as supplied by publisher]
Source: Algorithms for Molecular Biology : AMB - October 18, 2009 Category: Molecular Biology Authors: Zahoranszky LA, Katona GY, Hari P, Malnasi-Csizmadia A, Zweig KA, Zahoranszky-Kohalmi G Tags: Algorithms Mol Biol Source Type: journals
Strategies for targeted nonviral delivery of siRNAs in vivo.Email this article to a colleague.
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Silencing specific gene expression by RNA interference (RNAi) has rapidly become a standard tool for the reverse genetic analysis of gene functions. It also has tremendous potential for managing diseases for which effective treatment is currently unavailable or suboptimal. However, the poor cellular uptake of synthetic small interfering RNAs (siRNAs) is a major impediment for their clinical use. Great progress has been made in recent years to overcome this barrier, and several methods have been described for the in vivo delivery of siRNA. Moreover, the latest advances have focused on achieving targeted siRNA delivery r...
Source: Trends in Molecular Medicine - October 18, 2009 Category: Molecular Biology Authors: Kim SS, Garg H, Joshi A, Manjunath N Tags: Trends Mol Med Source Type: journals
The role of tenascin-C in tissue injury and tumorigenesis.Email this article to a colleague.
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The extracellular matrix molecule tenascin-C is highly expressed during embryonic development, tissue repair and in pathological situations such as chronic inflammation and cancer. Tenascin-C interacts with several other extracellular matrix molecules and cell-surface receptors, thus affecting tissue architecture, tissue resilience and cell responses. Tenascin-C modulates cell migration, proliferation and cellular signaling through induction of pro-inflammatory cytokines and oncogenic signaling molecules amongst other mechanisms. Given the causal role of inflammation in cancer progression, common mechanisms might be co...
Source: Journal of Cell Communication and Signaling - October 16, 2009 Category: Molecular Biology Authors: Midwood KS, Orend G Tags: J Cell Commun Signal Source Type: journals
α- and β-Adducin polymorphisms affect podocyte proteins and proteinuria in rodents and decline of renal function in human IgA nephropathyEmail this article to a colleague.
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Abstract Adducins are cytoskeletal actin-binding proteins (α, β, γ) that function as heterodimers and heterotetramers and are encoded
by distinct genes. Experimental and clinical evidence implicates α- and β-adducin variants in hypertension and renal dysfunction.
Here, we have addressed the role of α- and β-adducin on glomerular function and disease using β-adducin null mice, congenic
substrains for α- and β-adducin from the Milan hypertensive (MHS) and Milan normotensive (MNS) rats and patients with IgA
nephropathy. Targeted deletion of β-adducin in mice reduced urinary protein excretion, precede...
Source: Journal of Molecular Medicine - October 16, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Translating the oxidative stress hypothesis into the clinic: NOX versus NOSEmail this article to a colleague.
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Abstract Cardiovascular diseases remain the leading cause of death in industrialised nations. Since the pathomechanisms of most cardiovascular
diseases are not understood, the majority of therapeutic approaches are symptom-orientated. Knowing the molecular mechanism
of disease would enable more targeted therapies. One postulated underlying mechanism of cardiovascular diseases is oxidative
stress, i.e. the increased occurrence of reactive oxygen species such as superoxide. Oxidative stress leads to a dysfunction
of vascular endothelium-dependent protective mechanisms. There is growing evidence that this scena...
Source: Journal of Molecular Medicine - October 16, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
A novel imaging-based high-throughput screening approach to anti-angiogenic drug discoveryEmail this article to a colleague.
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The successful progression to the clinic of angiogenesis inhibitors for cancer treatment has spurred interest in developing new classes of anti-angiogenic compounds. The resulting surge in available candidate therapeutics highlights the need for robust, high-throughput angiogenesis screening systems that adequately capture the complexity of new vessel formation while providing quantitative evaluation of the potency of these agents. Available in vitro angiogenesis assays are either cumbersome, impeding adaptation to high-throughput screening formats, or inadequately model the complex multistep process of new vessel formatio...
Source: Cytometry Part A - October 15, 2009 Category: Molecular Biology Authors: Lasse Evensen, David R. Micklem, Wolfgang Link, James B. Lorens Source Type: journals
CD146+ T lymphocytes are increased in both the peripheral circulation and in the synovial effusions of patients with various musculoskeletal diseases and display pro-inflammatory gene profilesEmail this article to a colleague.
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Twenty-eight synovial effusions (SE) were obtained from 24 patients, paired samples of peripheral blood (PB) from 10 of these patients, and PB from 36 healthy individuals for analysis of CD146 on T-lymphocytes by flow cytometry. CD146+ or CD146- T-lymphocytes were sorted from three SE to study gene expression profiles and selected genes revalidated using QPCR assays. We found more CD3+CD146+ and CD4+CD146+ T-lymphocytes in PB from patients compared with PB of healthy individuals (4.71% ± 2.48% vs. 2.53% ± 1.08%, P = 0.028) and (6.29% ± 2.74% vs. 2.41% ± 0.96%, P = 0.0017), respectively, whereas CD8+CD146+ T-lymphocytes...
Source: Cytometry Part B: Clinical Cytometry - October 15, 2009 Category: Molecular Biology Authors: Pradeep Kumar Dagur, Gulnaz Tatlici, Mark Gourley, Leigh Samsel, Nalini Raghavachari, Poching Liu, Delong Liu, J. Philip McCoy Jr. Source Type: journals
The use of network analyses for elucidating mechanisms in cardiovascular diseaseEmail this article to a colleague.
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Diego Diez, Asa M. Wheelock, Susumu Goto, Jesper Z. Haeggstrom, Gabrielle Paulsson-Berne, Goran K. Hansson, Ulf Hedin, Anders Gabrielsen, Craig E. Wheelock
(Review from Mol. BioSyst.)
Diego Diez, Mol. BioSyst., 2010, DOI: 10.1039/b912078e
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Source: RSC - Mol. BioSyst. latest articles - October 15, 2009 Category: Molecular Biology Authors: Diego DiezAsa M. Wheelock Susumu Goto Jesper Z. Haeggstrom Gabrielle Paulsson-Berne Goran K. Hansson Ulf Hedin Anders Gabrielsen Craig E. Wheelock Source Type: journals
Facile syntheses of (+)-gabosines A, D, and EEmail this article to a colleague.
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Tony K. M. Shing, Hau M. Cheng
(Paper from Org. Biomol. Chem.)
Tony K. M. Shing, Org. Biomol. Chem., 2009, DOI: 10.1039/b911810a
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Source: RSC - Organic Biomolecular Chemistry - October 15, 2009 Category: Molecular Biology Authors: Tony K. M. ShingHau M. Cheng Source Type: journals
Plastid genomes of two brown algae, Ectocarpus siliculosus and Fucus vesiculosus: further insights on the evolution of red-algal derived plastidsEmail this article to a colleague.
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Conclusions:
The phylogenetic studies using concatenated plastid proteins still do not resolve the question of the monophyly of all chromist plastids. However, these results support both the monophyly of heterokont plastids and that of cryptophyte and haptophyte plastids, in agreement with nuclear phylogenies.
Source: BMC Evolutionary Biology - Latest articles - October 15, 2009 Category: Molecular Biology Authors: Gildas Le CorguilleGareth PearsonMarta ValenteCarla ViegasBernhard GschloesslErwan CorreXavier BaillyAkira PetersClaire JubinBenoit VacherieJ. Mark CockCatherine Leblanc Source Type: journals
Tissue-specific distribution of uncoupling proteins in normal rats and rats with high-fat-diet-induced obesity.Email this article to a colleague.
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Uncoupling proteins (UCPs) are a proton transporter family located in the mitochondrial inner membrane. Thus far, five molecules (UCP1-UCP5) have been identified as members of the UCP family. Recently, UCPs have attracted considerable interest in research on energy metabolism and obesity. However, to date, no study has focused on a comprehensive and systematic evaluation of the tissue-specific distribution of UCPs in obese individuals. Our study presents evidence of differential tissue expression profiles of five isoforms of UCPs in normal and diet-induced obese (DIO) rats using real-time polymerase chain reaction (PCR...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Zhang CM, Gu Y, Qing DN, Zhu JG, Zhu C, Zhang M, Guo XR Tags: Mol Biol Rep Source Type: journals
Investigation of LDHA and COPB1 as candidate genes for muscle development in the MYOD1 region of pig chromosome 2.Email this article to a colleague.
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Porcine MYOD1 gene has been mapped to swine chromosome (SSC) 2p14-p17, which is involved in the regulation of the proliferation and differentiation of skeletal muscle cells. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1) genes, which map close to MYOD1, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. However, little is known about the porcine LDHA and COPB1 genes. In the present study, the full-length cDNA of these two genes were cloned. The mapping results demonstrated that porcine LDHA and COPB1 we...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Qiu H, Xu X, Fan B, Rothschild MF, Martin Y, Liu B Tags: Mol Biol Rep Source Type: journals
A sticky situation: CCN1 promotes both proliferation and apoptosis of cancer cells.Email this article to a colleague.
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Members of the CCN family of matricellular signaling regulators promote cell adhesion through integrins and heparan sulfate-containing proteoglycans. A paradox of the CCN field is that, depending on the set of circumstances examined, individual CCN molecules can have quite different, and often opposing, effects. In a recent report, Franzen and colleagues (Mol Cancer Res. 7:1045-1055, 2009) show using siRNA knockdown that CCN1 (cyr61) is essential for the proliferation of prostate cancer cells. Intriguingly, on the other hand, CCN1 also enhances TRAIL-induced apoptosis. Thus the utility of anti-CCN1 therapy in cancer ne...
Source: Journal of Cell Communication and Signaling - October 15, 2009 Category: Molecular Biology Authors: Leask A Tags: J Cell Commun Signal Source Type: journals
The interaction of Thrombospondins with extracellular matrix proteins.Email this article to a colleague.
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The thrombospondins (TSPs) are a family of five matricellular proteins that appear to function as adapter molecules to guide extracellular matrix synthesis and tissue remodeling in a variety of normal and disease settings. Various TSPs have been shown to bind to fibronectin, laminin, matrilins, collagens and other extracellular matrix (ECM) proteins. The importance of TSP-1 in this context is underscored by the fact that it is rapidly deposited at the sites of tissue damage by platelets. An association of TSPs with collagens has been known for over 25 years. The observation that the disruption of the TSP-2 gene in mice...
Source: Journal of Cell Communication and Signaling - October 15, 2009 Category: Molecular Biology Authors: Tan K, Lawler J Tags: J Cell Commun Signal Source Type: journals
Proteomic analysis of A33-immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein signature.Email this article to a colleague.
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Exosomes are 40-100 nm diameter nanovesicles of endocytic origin that are released from diverse cell types. To better understand the biological role of exosomes and to avoid confounding data arising from proteinaceous contaminants, it is important to work with highly-purified material. Here, we describe an immunoaffinity-capture method using the colon epithelial cell specific A33 antibody to purify colorectal cancer cell (LIM1215) -derived exosomes. LC-MS/MS revealed 394 unique exosomal proteins of which 112 proteins (28%) contained signal peptides and a significant enrichment of proteins containing coiled-coil, RAS an...
Source: Molecular and Cellular Proteomics : MCP - October 15, 2009 Category: Molecular Biology Authors: Mathivanan S, Lim JW, Tauro BJ, Ji H, Moritz RL, Simpson RJ Tags: Mol Cell Proteomics Source Type: journals
Performance metrics for liquid chromatography-tandem mass spectrometry systems in proteomic analyses and evaluation by the CPTAC network.Email this article to a colleague.
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Wang P, Whiteaker JR, Zimmerman LJ, Carr SA, Fisher SJ, Gibson BW, Paulovich AG, Regnier FE, Rodriguez H
A major unmet need in liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analyses is a set of tools for quantitative assessment of system performance and evaluation of technical variability. Here we describe 46 system performance metrics for monitoring chromatographic performance, electrospray source stability, MS1 and MS2 signals, dynamic sampling of ions for MS/MS and peptide identification. Applied to datasets from replicate LC-MS/MS analyses, these metrics display consistent, reasonable r...
Source: Molecular and Cellular Proteomics : MCP - October 15, 2009 Category: Molecular Biology Authors: Rudnick PA, Clauser KR, Kilpatrick LE, Tchekhovskoi DV, Neta P, Blonder N, Billheimer DD, Blackman RK, Bunk DM, Cardasis HL, Ham AJ, Jaffe JD, Kinsinger CR, Mesri M, Neubert TA, Schilling B, Tabb DL, Tegeler TJ, Vega-Montoto L, Mulayath Variyath A, Wang M Tags: Mol Cell Proteomics Source Type: journals
p53 Promotes proteasome-dependent degradation of oncogenic protein HBx by transcription of MDM2.Email this article to a colleague.
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In this study, we attempted to identify the underlying mechanism. We found that overexpression of p53 protein reduces the level of HBx protein and shortens its half-life, however, in MDM2 knock out cells, p53 has no effects on degradation of HBx, meanwhile, overexpression of MDM2 in absence of p53 can accelerate turnover of HBx protein. These indicate that p53-mediated HBx degradation is MDM2-dependent. MDM2 interacts with HBx in vitro and in vivo but does not promote its ubiquitination. In consistent with the results above, HCC tissue samples with wild-type p53 hardly detect HBx protein, whereas, HBx always accumulate in ...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Xian L, Zhao J, Wang J, Fang Z, Peng B, Wang W, Ji X, Yu L Tags: Mol Biol Rep Source Type: journals
An examination of aspartate decarboxylase and glutamate decarboxylase activity in mosquitoes.Email this article to a colleague.
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In this study, we expressed two putative mosquito glutamate decarboxylase-like enzymes of mosquitoes and critically analyzed their substrate specificity and biochemical properties. Our results provide clear biochemical evidence establishing that one of them is an aspartate decarboxylase and the other is a glutamate decarboxylase. The mosquito aspartate decarboxylase functions exclusively on the production of beta-alanine with no activity with glutamate. Likewise the mosquito glutamate decarboxylase is highly specific to glutamate with essentially no activity with aspartate. Although insect aspartate decarboxylase shares hi...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Richardson G, Ding H, Rocheleau T, Mayhew G, Reddy E, Han Q, Christensen BM, Li J Tags: Mol Biol Rep Source Type: journals
Identification of HSP27 as a potential tumor marker for colorectal cancer by the two-dimensional polyacrylamide gel electrophoresis.Email this article to a colleague.
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This study was designed to use comparative proteomics technology to find the differentially expressed proteins between human colorectal carcinoma and the corresponding normal tumor-adjacent colorectal tissues. We have used the highly sensitive two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) for the identification of proteins differentially expressed in tumoral and neighboring normal mucosa. We have detected differences in abundance of 42 proteins with statistical variance of the tumor versus normal spot volume ratio within t...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Liu W, Ma Y, Huang L, Peng J, Zhang P, Zhang H, Chen J, Qin H Tags: Mol Biol Rep Source Type: journals
Co-treating with arecoline and 4-nitroquinoline 1-oxide to establish a mouse model mimicking oral tumorigenesis.Email this article to a colleague.
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In conclusion, we have established a powerful animal model that enables the study of the promoting effects of arecoline on tongue tumorigenesis. Data subsequently attained from this mouse model support a role for alphaB-crystallin and Hsp27 as clinical markers for tumor progression.
PMID: 19840781 [PubMed - as supplied by publisher]
Source: Chemico-Biological Interactions - October 15, 2009 Category: Molecular Biology Authors: Chang NW, Pei RJ, Tseng HC, Yeh KT, Chan HC, Lee MR, Lin C, Hsieh WT, Kao MC, Tsai MH, Lin CF Tags: Chem Biol Interact Source Type: journals
Androgen receptor coactivator p44/Mep50 in breast cancer growth and invasion.Email this article to a colleague.
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In this report, we examined the expression and function of p44 in breast cancer. In addition to being an AR coactivator, p44 also functions as an ER coactivator. In contrast to findings in prostate cancer, the expression of p44 shows strong cytoplasmic expression in morphologically normal terminal ductal lobular units, while nuclear p44 is observed in both ductal carcinoma in situ (DCIS) and invasive carcinoma. Further, overexpression of nuclear-localized p44 stimulates proliferation and invasion in MCF7 breast cancer cells in the presence of estrogen and is ERalpha-dependent. These findings strongly suggest that p44 plays...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Peng Y, Li Y, Gellert LL, Zou X, Wang J, Singh B, Xu R, Chiriboga L, Daniels G, Pan R, Zhang DY, Garabedian M, Schneider R, Wang Z, Lee P Tags: J Cell Mol Med Source Type: journals
Glomerular parietal epithelial cells of adult murine kidney undergo EMT to generate cells with traits of renal progenitors.Email this article to a colleague.
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ABSTRACT Glomerular parietal epithelial cells (GPECs) are known to revert to embryonic phenotype in response to renal injury. However, the mechanism of de-differentiation in GPECs and the underlying cellular processes are not fully understood. In the present study, we show that cultured GPECs of adult murine kidney undergo epithelial-mesenchymal transition (EMT) to generate cells, which express CD24, CD44 and CD29 surface antigens. Characterization by qRT-PCR and immunostaining of these clonogenic cells demonstrate that they exhibit metastable phenotype with co-expression of both epithelial (cytokeratin-18) and mesench...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Chandra V, Phadnis S, Bhonde R Tags: J Cell Mol Med Source Type: journals
FHL1 interacts with estrogen receptors and regulates breast cancer cell growth.Email this article to a colleague.
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Abstract Four and a half LIM protein 1 (FHL1) belongs to the LIM-only protein family and plays important roles in muscle growth and carcinogenesis. However, the biological function of FHL1 remains largely unknown. Here, we show that FHL1 physically and functionally interacted with estrogen receptors (ERs), which are involved in breast cancer development and progression. FHL1 bound specifically to the activation function-1 (AF1) domain of ER. Physical interaction of FHL1 and ER is required for FHL1 repression of estrogen-responsive gene transcription. FHL1 affected recruitment of ER to an estrogen-responsive promoter an...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Ding L, Niu C, Zheng Y, Xiong Z, Liu Y, Lin J, Sun H, Huang K, Yang W, Li X, Ye Q Tags: J Cell Mol Med Source Type: journals
Plasma gelsolin facilitates interaction between beta(2) glycoprotein I and alpha5beta1 integrin.Email this article to a colleague.
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In this study, we identified plasma gelsolin as a protein associated with beta(2)GPI by using immunoaffinity chromatography and mass spectrometric analysis. An in vivo binding assay showed that endogenous beta(2)GPI interacts with plasma gelsolin, which binds to integrin alpha5beta1 through fibronectin. The tethering of beta(2)GPI to monoclonal anti-beta(2)GPI autoantibody on the cell surface was enhanced in the presence of plasma gelsolin. Immunoblot analysis demonstrated that p38 MAPK protein was phosphorylated by monoclonal anti-beta(2)GPI antibody treatment, and its phosphorylation was attenuated in the presence of ant...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Bohgaki M, Matsumoto M, Atsumi T, Kondo T, Yasuda S, Horita T, Nakayama KI, Okumura F, Hatakeyama S, Koike T Tags: J Cell Mol Med Source Type: journals
A systems biology perspective on sVEGFR1: its biological function, pathogenic role & therapeutic use.Email this article to a colleague.
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A systems biology perspective on sVEGFR1: its biological function, pathogenic role & therapeutic use.
J Cell Mol Med. 2009 Oct 16;
Authors: Wu FT, Stefanini MO, Gabhann FM, Kontos CD, Annex BH, Popel AS
Abstract Angiogenesis is the growth of new capillaries from pre-existent microvasculature. A wide range of pathological conditions, from atherosclerosis to cancer, can be attributed to either excessive or deficient angiogenesis. Central to the physiological regulation of angiogenesis is the vascular endothelial growth factor (VEGF) system - its ligands and receptors (VEGFRs) are thus prime molecular targets ...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Wu FT, Stefanini MO, Gabhann FM, Kontos CD, Annex BH, Popel AS Tags: J Cell Mol Med Source Type: journals
Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells.Email this article to a colleague.
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ABSTRACT Aldehyde dehydrogenases (ALDH) are a family of enzymes that efficiently detoxify aldehydic products generated by reactive oxygen species and might therefore participate in cell survival. Since ALDH activity has been used to identify normal and malignant cells with stem cell properties, we asked whether human myogenic precursor cells (myoblasts) could be identified and isolated based on their levels of ALDH activity. Human muscle explant-derived cells were incubated with ALDEFLUOR, a fluorescent substrate for ALDH, and we determined by flow cytometry the level of enzyme activity. We found that ALDH activity pos...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Jean E, Laoudj-Chenivesse D, Notarnicola C, Rouger K, Serratrice N, Bonnieu A, Gay S, Bacou F, Duret C, Carnac G Tags: J Cell Mol Med Source Type: journals
Ankyrin protein networks in membrane formation and stabilization.Email this article to a colleague.
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Abstract In eukaryotic cells, ankyrins serve as adaptor proteins that link membrane proteins to the underlying cytoskeleton. These adaptor proteins form protein complexes consisting of integral membrane proteins, signaling molecules, and cytoskeletal components. With their modular architecture and ability to interact with many proteins, ankyrins organize and stabilize these protein networks, thereby establishing the infrastructure of membrane domains with specialized functions. To this end, ankyrin collaborates with a number of proteins including cytoskeletal proteins, cell adhesion molecules, and large structural prot...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Cunha SR, Mohler PJ Tags: J Cell Mol Med Source Type: journals
Assessment of tumor hypoxia for prediction of response to therapy and cancer prognosis.Email this article to a colleague.
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Abstract Tumor cells exploit both genetic and adaptive means to survive and proliferate in hypoxic microenvironments, resulting in the outgrowth of more aggressive tumor cell clones. Direct measurements of tumor oxygenation, and surrogate markers of the hypoxic response in tumors (for instance, hypoxia inducible factor-1alpha, carbonic anhydrase 9 and glucose transporter-1) are well-established prognostic markers in solid cancers. However, individual markers do not fully capture the complex, dynamic and heterogeneous hypoxic response in cancer. To overcome this, expression profiling has been employed to identify hypoxi...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Jubb AM, Buffa FM, Harris AL Tags: J Cell Mol Med Source Type: journals
Telomere attrition and genomic instability in xeroderma pigmentosum type-B deficient fibroblasts under oxidative stress.Email this article to a colleague.
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ABSTRACT Xeroderma Pigmentosum B (XPB/ ERCC3/ p89) is an ATP-dependent 3'-->5' directed DNA helicase involved in basal RNA transcription and the nucleotide excision repair pathway (NER). While the role of NER in alleviating oxidative DNA damage has been acknowledged it remains poorly understood. To study the involvement of XPB in repair of oxidative DNA damage, we utilised primary fibroblasts from a patient suffering from Xeroderma Pigmentosum with Cockayne Syndrome and Hydrogen Peroxide (H(2)O(2)) to induce oxidative stress. Mutant cells retained higher viability and cell cycle dysfunction after H(2)O(2) exposure. ...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Ting AP, Low GK, Gopalakrishnan K, Hande MP Tags: J Cell Mol Med Source Type: journals
