Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 13.
A sticky situation: CCN1 promotes both proliferation and apoptosis of cancer cells.
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Members of the CCN family of matricellular signaling regulators promote cell adhesion through integrins and heparan sulfate-containing proteoglycans. A paradox of the CCN field is that, depending on the set of circumstances examined, individual CCN molecules can have quite different, and often opposing, effects. In a recent report, Franzen and colleagues (Mol Cancer Res. 7:1045-1055, 2009) show using siRNA knockdown that CCN1 (cyr61) is essential for the proliferation of prostate cancer cells. Intriguingly, on the other hand, CCN1 also enhances TRAIL-induced apoptosis. Thus the utility of anti-CCN1 therapy in cancer ne...
Source: Journal of Cell Communication and Signaling - October 15, 2009 Category: Molecular Biology Authors: Leask A Tags: J Cell Commun Signal Source Type: journals
The interaction of Thrombospondins with extracellular matrix proteins.Email this article to a colleague.
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The thrombospondins (TSPs) are a family of five matricellular proteins that appear to function as adapter molecules to guide extracellular matrix synthesis and tissue remodeling in a variety of normal and disease settings. Various TSPs have been shown to bind to fibronectin, laminin, matrilins, collagens and other extracellular matrix (ECM) proteins. The importance of TSP-1 in this context is underscored by the fact that it is rapidly deposited at the sites of tissue damage by platelets. An association of TSPs with collagens has been known for over 25 years. The observation that the disruption of the TSP-2 gene in mice...
Source: Journal of Cell Communication and Signaling - October 15, 2009 Category: Molecular Biology Authors: Tan K, Lawler J Tags: J Cell Commun Signal Source Type: journals
Proteomic analysis of A33-immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein signature.Email this article to a colleague.
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Exosomes are 40-100 nm diameter nanovesicles of endocytic origin that are released from diverse cell types. To better understand the biological role of exosomes and to avoid confounding data arising from proteinaceous contaminants, it is important to work with highly-purified material. Here, we describe an immunoaffinity-capture method using the colon epithelial cell specific A33 antibody to purify colorectal cancer cell (LIM1215) -derived exosomes. LC-MS/MS revealed 394 unique exosomal proteins of which 112 proteins (28%) contained signal peptides and a significant enrichment of proteins containing coiled-coil, RAS an...
Source: Molecular and Cellular Proteomics : MCP - October 15, 2009 Category: Molecular Biology Authors: Mathivanan S, Lim JW, Tauro BJ, Ji H, Moritz RL, Simpson RJ Tags: Mol Cell Proteomics Source Type: journals
Performance metrics for liquid chromatography-tandem mass spectrometry systems in proteomic analyses and evaluation by the CPTAC network.Email this article to a colleague.
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Wang P, Whiteaker JR, Zimmerman LJ, Carr SA, Fisher SJ, Gibson BW, Paulovich AG, Regnier FE, Rodriguez H
A major unmet need in liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analyses is a set of tools for quantitative assessment of system performance and evaluation of technical variability. Here we describe 46 system performance metrics for monitoring chromatographic performance, electrospray source stability, MS1 and MS2 signals, dynamic sampling of ions for MS/MS and peptide identification. Applied to datasets from replicate LC-MS/MS analyses, these metrics display consistent, reasonable r...
Source: Molecular and Cellular Proteomics : MCP - October 15, 2009 Category: Molecular Biology Authors: Rudnick PA, Clauser KR, Kilpatrick LE, Tchekhovskoi DV, Neta P, Blonder N, Billheimer DD, Blackman RK, Bunk DM, Cardasis HL, Ham AJ, Jaffe JD, Kinsinger CR, Mesri M, Neubert TA, Schilling B, Tabb DL, Tegeler TJ, Vega-Montoto L, Mulayath Variyath A, Wang M Tags: Mol Cell Proteomics Source Type: journals
p53 Promotes proteasome-dependent degradation of oncogenic protein HBx by transcription of MDM2.Email this article to a colleague.
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In this study, we attempted to identify the underlying mechanism. We found that overexpression of p53 protein reduces the level of HBx protein and shortens its half-life, however, in MDM2 knock out cells, p53 has no effects on degradation of HBx, meanwhile, overexpression of MDM2 in absence of p53 can accelerate turnover of HBx protein. These indicate that p53-mediated HBx degradation is MDM2-dependent. MDM2 interacts with HBx in vitro and in vivo but does not promote its ubiquitination. In consistent with the results above, HCC tissue samples with wild-type p53 hardly detect HBx protein, whereas, HBx always accumulate in ...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Xian L, Zhao J, Wang J, Fang Z, Peng B, Wang W, Ji X, Yu L Tags: Mol Biol Rep Source Type: journals
An examination of aspartate decarboxylase and glutamate decarboxylase activity in mosquitoes.Email this article to a colleague.
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In this study, we expressed two putative mosquito glutamate decarboxylase-like enzymes of mosquitoes and critically analyzed their substrate specificity and biochemical properties. Our results provide clear biochemical evidence establishing that one of them is an aspartate decarboxylase and the other is a glutamate decarboxylase. The mosquito aspartate decarboxylase functions exclusively on the production of beta-alanine with no activity with glutamate. Likewise the mosquito glutamate decarboxylase is highly specific to glutamate with essentially no activity with aspartate. Although insect aspartate decarboxylase shares hi...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Richardson G, Ding H, Rocheleau T, Mayhew G, Reddy E, Han Q, Christensen BM, Li J Tags: Mol Biol Rep Source Type: journals
Identification of HSP27 as a potential tumor marker for colorectal cancer by the two-dimensional polyacrylamide gel electrophoresis.Email this article to a colleague.
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This study was designed to use comparative proteomics technology to find the differentially expressed proteins between human colorectal carcinoma and the corresponding normal tumor-adjacent colorectal tissues. We have used the highly sensitive two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) for the identification of proteins differentially expressed in tumoral and neighboring normal mucosa. We have detected differences in abundance of 42 proteins with statistical variance of the tumor versus normal spot volume ratio within t...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Liu W, Ma Y, Huang L, Peng J, Zhang P, Zhang H, Chen J, Qin H Tags: Mol Biol Rep Source Type: journals
Co-treating with arecoline and 4-nitroquinoline 1-oxide to establish a mouse model mimicking oral tumorigenesis.Email this article to a colleague.
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In conclusion, we have established a powerful animal model that enables the study of the promoting effects of arecoline on tongue tumorigenesis. Data subsequently attained from this mouse model support a role for alphaB-crystallin and Hsp27 as clinical markers for tumor progression.
PMID: 19840781 [PubMed - as supplied by publisher]
Source: Chemico-Biological Interactions - October 15, 2009 Category: Molecular Biology Authors: Chang NW, Pei RJ, Tseng HC, Yeh KT, Chan HC, Lee MR, Lin C, Hsieh WT, Kao MC, Tsai MH, Lin CF Tags: Chem Biol Interact Source Type: journals
Androgen receptor coactivator p44/Mep50 in breast cancer growth and invasion.Email this article to a colleague.
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In this report, we examined the expression and function of p44 in breast cancer. In addition to being an AR coactivator, p44 also functions as an ER coactivator. In contrast to findings in prostate cancer, the expression of p44 shows strong cytoplasmic expression in morphologically normal terminal ductal lobular units, while nuclear p44 is observed in both ductal carcinoma in situ (DCIS) and invasive carcinoma. Further, overexpression of nuclear-localized p44 stimulates proliferation and invasion in MCF7 breast cancer cells in the presence of estrogen and is ERalpha-dependent. These findings strongly suggest that p44 plays...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Peng Y, Li Y, Gellert LL, Zou X, Wang J, Singh B, Xu R, Chiriboga L, Daniels G, Pan R, Zhang DY, Garabedian M, Schneider R, Wang Z, Lee P Tags: J Cell Mol Med Source Type: journals
Glomerular parietal epithelial cells of adult murine kidney undergo EMT to generate cells with traits of renal progenitors.Email this article to a colleague.
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ABSTRACT Glomerular parietal epithelial cells (GPECs) are known to revert to embryonic phenotype in response to renal injury. However, the mechanism of de-differentiation in GPECs and the underlying cellular processes are not fully understood. In the present study, we show that cultured GPECs of adult murine kidney undergo epithelial-mesenchymal transition (EMT) to generate cells, which express CD24, CD44 and CD29 surface antigens. Characterization by qRT-PCR and immunostaining of these clonogenic cells demonstrate that they exhibit metastable phenotype with co-expression of both epithelial (cytokeratin-18) and mesench...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Chandra V, Phadnis S, Bhonde R Tags: J Cell Mol Med Source Type: journals
FHL1 interacts with estrogen receptors and regulates breast cancer cell growth.Email this article to a colleague.
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Abstract Four and a half LIM protein 1 (FHL1) belongs to the LIM-only protein family and plays important roles in muscle growth and carcinogenesis. However, the biological function of FHL1 remains largely unknown. Here, we show that FHL1 physically and functionally interacted with estrogen receptors (ERs), which are involved in breast cancer development and progression. FHL1 bound specifically to the activation function-1 (AF1) domain of ER. Physical interaction of FHL1 and ER is required for FHL1 repression of estrogen-responsive gene transcription. FHL1 affected recruitment of ER to an estrogen-responsive promoter an...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Ding L, Niu C, Zheng Y, Xiong Z, Liu Y, Lin J, Sun H, Huang K, Yang W, Li X, Ye Q Tags: J Cell Mol Med Source Type: journals
Plasma gelsolin facilitates interaction between beta(2) glycoprotein I and alpha5beta1 integrin.Email this article to a colleague.
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In this study, we identified plasma gelsolin as a protein associated with beta(2)GPI by using immunoaffinity chromatography and mass spectrometric analysis. An in vivo binding assay showed that endogenous beta(2)GPI interacts with plasma gelsolin, which binds to integrin alpha5beta1 through fibronectin. The tethering of beta(2)GPI to monoclonal anti-beta(2)GPI autoantibody on the cell surface was enhanced in the presence of plasma gelsolin. Immunoblot analysis demonstrated that p38 MAPK protein was phosphorylated by monoclonal anti-beta(2)GPI antibody treatment, and its phosphorylation was attenuated in the presence of ant...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Bohgaki M, Matsumoto M, Atsumi T, Kondo T, Yasuda S, Horita T, Nakayama KI, Okumura F, Hatakeyama S, Koike T Tags: J Cell Mol Med Source Type: journals
A systems biology perspective on sVEGFR1: its biological function, pathogenic role & therapeutic use.Email this article to a colleague.
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A systems biology perspective on sVEGFR1: its biological function, pathogenic role & therapeutic use.
J Cell Mol Med. 2009 Oct 16;
Authors: Wu FT, Stefanini MO, Gabhann FM, Kontos CD, Annex BH, Popel AS
Abstract Angiogenesis is the growth of new capillaries from pre-existent microvasculature. A wide range of pathological conditions, from atherosclerosis to cancer, can be attributed to either excessive or deficient angiogenesis. Central to the physiological regulation of angiogenesis is the vascular endothelial growth factor (VEGF) system - its ligands and receptors (VEGFRs) are thus prime molecular targets ...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Wu FT, Stefanini MO, Gabhann FM, Kontos CD, Annex BH, Popel AS Tags: J Cell Mol Med Source Type: journals
Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells.Email this article to a colleague.
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ABSTRACT Aldehyde dehydrogenases (ALDH) are a family of enzymes that efficiently detoxify aldehydic products generated by reactive oxygen species and might therefore participate in cell survival. Since ALDH activity has been used to identify normal and malignant cells with stem cell properties, we asked whether human myogenic precursor cells (myoblasts) could be identified and isolated based on their levels of ALDH activity. Human muscle explant-derived cells were incubated with ALDEFLUOR, a fluorescent substrate for ALDH, and we determined by flow cytometry the level of enzyme activity. We found that ALDH activity pos...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Jean E, Laoudj-Chenivesse D, Notarnicola C, Rouger K, Serratrice N, Bonnieu A, Gay S, Bacou F, Duret C, Carnac G Tags: J Cell Mol Med Source Type: journals
Ankyrin protein networks in membrane formation and stabilization.Email this article to a colleague.
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Abstract In eukaryotic cells, ankyrins serve as adaptor proteins that link membrane proteins to the underlying cytoskeleton. These adaptor proteins form protein complexes consisting of integral membrane proteins, signaling molecules, and cytoskeletal components. With their modular architecture and ability to interact with many proteins, ankyrins organize and stabilize these protein networks, thereby establishing the infrastructure of membrane domains with specialized functions. To this end, ankyrin collaborates with a number of proteins including cytoskeletal proteins, cell adhesion molecules, and large structural prot...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Cunha SR, Mohler PJ Tags: J Cell Mol Med Source Type: journals
Assessment of tumor hypoxia for prediction of response to therapy and cancer prognosis.Email this article to a colleague.
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Abstract Tumor cells exploit both genetic and adaptive means to survive and proliferate in hypoxic microenvironments, resulting in the outgrowth of more aggressive tumor cell clones. Direct measurements of tumor oxygenation, and surrogate markers of the hypoxic response in tumors (for instance, hypoxia inducible factor-1alpha, carbonic anhydrase 9 and glucose transporter-1) are well-established prognostic markers in solid cancers. However, individual markers do not fully capture the complex, dynamic and heterogeneous hypoxic response in cancer. To overcome this, expression profiling has been employed to identify hypoxi...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Jubb AM, Buffa FM, Harris AL Tags: J Cell Mol Med Source Type: journals
Telomere attrition and genomic instability in xeroderma pigmentosum type-B deficient fibroblasts under oxidative stress.Email this article to a colleague.
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ABSTRACT Xeroderma Pigmentosum B (XPB/ ERCC3/ p89) is an ATP-dependent 3'-->5' directed DNA helicase involved in basal RNA transcription and the nucleotide excision repair pathway (NER). While the role of NER in alleviating oxidative DNA damage has been acknowledged it remains poorly understood. To study the involvement of XPB in repair of oxidative DNA damage, we utilised primary fibroblasts from a patient suffering from Xeroderma Pigmentosum with Cockayne Syndrome and Hydrogen Peroxide (H(2)O(2)) to induce oxidative stress. Mutant cells retained higher viability and cell cycle dysfunction after H(2)O(2) exposure. ...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Ting AP, Low GK, Gopalakrishnan K, Hande MP Tags: J Cell Mol Med Source Type: journals
Extracellular matrix assembly and organization during zebrafish gastrulation.Email this article to a colleague.
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Zebrafish gastrulation entails morphogenetic cell movements that shape the body plan and give rise to an embryo with defined anterior-posterior and dorsal-ventral axes. Regulating these cell movements are diverse signaling pathways and proteins including Wnts, Src-family tyrosine kinases, cadherins, and matrix metalloproteinases. While our knowledge of how these proteins impact cell polarity and migration has advanced considerably in the last decade, almost no data exist regarding the organization of extracellular matrix (ECM) during zebrafish gastrulation. Here, we describe for the first time the assembly of a fibrone...
Source: Matrix Biology - October 15, 2009 Category: Molecular Biology Authors: Latimer A, Jessen JR Tags: Matrix Biol Source Type: journals
FAK mediates signal crosstalk between type II collagen and TGF-beta 1 cascades in chondrocytic cells.Email this article to a colleague.
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In this study, expression of pSMAD 2, pSMAD 3, pFAK(Y397) and pFAK(Y925) were synergistically increased by co-treatment with type II collagen and TGF-beta1 in articular chondrocytes. The proliferation of porcine articular chondrocytes and GAG secretion in SW1353 cells were synergistically increased by co-stimulation with type II collagen and TGF-beta1. Synergistically increased expression and nuclear translocation of pSMAD 2 and pSMAD 3 and GAG secretion of SW1353 cells were significantly inhibited by siFAK transfection. Therefore, we suggest that FAK-SMAD 2/3 mediates signal crosstalk between type II collagen and TGF-beta...
Source: Matrix Biology - October 15, 2009 Category: Molecular Biology Authors: Park MS, Kim YH, Lee JW Tags: Matrix Biol Source Type: journals
Evaluation of genome coverage and fidelity of multiple displacement amplification from single cells by SNP arrayEmail this article to a colleague.
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The scarce amount of DNA contained in a singe cell is a limiting factor for clinical application of preimplantation genetic diagnosis mainly due to the risk of misdiagnosis caused by allele dropout and the difficulty in obtaining copy number variations in all 23 pairs of chromosomes. Multiple displacement amplification (MDA) has been reported to generate large quantity of products from small amount of templates. Here, we evaluated the fidelity of whole-genome amplification MDA from single or a few cells and determined the accuracy of chromosome copy number assessment on these MDA products using an Affymetrix 10K 2.0 SNP Ma...
Source: Molecular Human Reproduction - October 15, 2009 Category: Molecular Biology Authors: Ling, J., Zhuang, G., Tazon-Vega, B., Zhang, C., Cao, B., Rosenwaks, Z., Xu, K. Tags: Original Research Source Type: journals
Regulation of soluble vascular endothelial growth factor receptor 1 secretion from human endothelial cells by tissue inhibitor of metalloproteinase 1Email this article to a colleague.
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In conclusion, our results provide evidence that TIMP-1 increases the production of sVEGFR-1 in endothelial cells and thus may reduce VEGF bioavailability, leading to reduced blood vessel growth in the ovary.
Source: Molecular Human Reproduction - October 15, 2009 Category: Molecular Biology Authors: Bruegmann, E., Gruemmer, R., Neulen, J., Motejlek, K. Tags: Original Research Source Type: journals
An RNA spiking method demonstrates that 18S rRNA is regulated by progesterone in the mouse uterusEmail this article to a colleague.
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Identifying suitable housekeeping genes for quantitative RT–PCR in the uterus is problematic, as this tissue undergoes significant structural and functional alterations during the oestrous cycle and pregnancy in response to circulating hormones. The suitability of 18S rRNA as a housekeeping gene in mouse uterus was investigated by introducing an ‘RNA spike’ standard into the reverse transcription reaction. 18S rRNA levels increased by Day 4 of pregnancy and after progesterone administration in ovariectomized mice. We conclude that 18S rRNA is not a suitable housekeeping gene for quantitative RT–PCR ...
Source: Molecular Human Reproduction - October 15, 2009 Category: Molecular Biology Authors: Craythorn, R.G., Girling, J.E., Hedger, M.P., Rogers, P.A.W., Winnall, W.R. Tags: Technical Note Source Type: journals
Recent progress in luteinizing hormone/human chorionic gonadotrophin hormone researchEmail this article to a colleague.
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The role of luteinizing hormone (LH) and human chorionic gonadotrophin hormone (hCG) in the regulation of normal reproductive functions in males and females is quite well established. Besides the use of hCG in the development of diagnostic immunoassays, it has been successfully used in the induction of final follicular maturation and ovulation in the assisted reproductive technologies. The basic and clinical research on the nongonadal actions of LH/hCG in the recent years has extended the potential of using these hormones in several clinical indications. Hereby we will analyze the advances in the LH/hCG research (briefly e...
Source: Molecular Human Reproduction - October 15, 2009 Category: Molecular Biology Authors: Rahman, N. A., Rao, C.V. Tags: New Research Horizon Reviews Source Type: journals
Leukocytes are primed in peripheral blood for activation during term and preterm labourEmail this article to a colleague.
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We hypothesized that the priming and activation of maternal leukocytes in peripheral blood is a key component of parturition, and that inappropriate preterm priming of leukocytes might initiate preterm labour and delivery. The purpose of this study was to characterize peripheral blood leukocyte activation during human term and preterm labour. We obtained blood samples from pregnant women at term and preterm, both in labour and not in labour. Leukocytes were characterized according to cell subtype and cell surface marker expression. Additionally, we quantified leukocyte cytokine mRNA production, migratory ability and reacti...
Source: Molecular Human Reproduction - October 15, 2009 Category: Molecular Biology Authors: Yuan, M., Jordan, F., McInnes, I.B., Harnett, M.M., Norman, J.E. Tags: Original Research Source Type: journals
Metastasis associated lung adenocarcinoma transcript 1 is up-regulated in placenta previa increta/percreta and strongly associated with trophoblast-like cell invasion in vitroEmail this article to a colleague.
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In this study, annealing control primer–polymerase chain reaction (ACP–PCR) was used to identify differentially expressed genes, which may impair placentation resulting in placenta previa I/P. Placental tissues from I/P and non-increta/percreta (non-I/P) sites were concomitantly collected from patients undergoing Cesarean hysterectomy. After ACP–PCR experiments (three patients), the differentially expressed bands, consistently showing up- or down-regulated trends between each of the I/P and non-I/P tissue pairs, were cloned and sequenced. Human non-protein coding metastasis associated lung adenocarcinoma ...
Source: Molecular Human Reproduction - October 15, 2009 Category: Molecular Biology Authors: Tseng, J.-J., Hsieh, Y.-T., Hsu, S.-L., Chou, M.-M. Tags: Original Research Source Type: journals
Mutations in the protamine locus: association with spermatogenic failure?Email this article to a colleague.
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The protamine locus consists of a 28.5 kb region with a linear array of the protamine (PRM)1, PRM2, PRM3 and transition nuclear protein (TNP)2 genes. Several studies indicate an abnormal expression pattern of protamine genes associated with male infertility, although the molecular mechanism underlying this observation is unclear. Here, we determined the spectrum of DNA variants present in all four genes in men with unexplained infertility compared with an ancestry-matched fertile/normospermic population. A total of 160 control individuals and at least 125 infertile men with either idiopathic azoospermia or oligozoospermia ...
Source: Molecular Human Reproduction - October 15, 2009 Category: Molecular Biology Authors: Imken, L., Rouba, H., El Houate, B., Louanjli, N., Barakat, A., Chafik, A., McElreavey, K. Tags: Original Research Source Type: journals
In Vivo Imaging of Sentinel Nodes Using Fluorescent Silica Nanoparticles in Living MiceEmail this article to a colleague.
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Conclusion Our data provide strong evidence that functionalized silica nanoparticles has a promising potential as organic lymphatic tracer
in biomedical imaging such as pre- and intraoperative surgical guidance.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0262-8Authors
Yong Hyun Jeon, Seoul National University College of Medicine Department of Nuclear Medicine 28 Yongon-dong, Jongno-gu Seoul 110-744 South KoreaYoung-Hwa Kim, Seoul National University Department of Laboratory of Molecular Imaging and Therapy of Cancer Research Institute Seoul South KoreaKihwan Choi, Seoul...
Source: Molecular Imaging and Biology - October 15, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
Efficient one-pot synthesis of 2,4-di(het)aryl and 2,4-diamino pyrido[3,2-d]pyrimidines involving regioselective SNAr and palladium-catalyzed reactionsEmail this article to a colleague.
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Abdellatif Tikad, Sylvain Routier, Mohamed Akssira, Gerald Guillaumet
(Paper from Org. Biomol. Chem.)
Abdellatif Tikad, Org. Biomol. Chem., 2009, DOI: 10.1039/b913657f
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Source: RSC - Organic Biomolecular Chemistry - October 14, 2009 Category: Molecular Biology Authors: Abdellatif TikadSylvain Routier Mohamed Akssira Gerald Guillaumet Source Type: journals
A post-modification approach to peptide foldamersEmail this article to a colleague.
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Nadja Franz, Laure Menin, Harm-Anton Klok
(Paper from Org. Biomol. Chem.)
Nadja Franz, Org. Biomol. Chem., 2009, DOI: 10.1039/b912313j
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Source: RSC - Organic Biomolecular Chemistry - October 14, 2009 Category: Molecular Biology Authors: Nadja FranzLaure Menin Harm-Anton Klok Source Type: journals
Robust preparation of novel imidazo[5,1-b][1,3,4]oxadiazolesEmail this article to a colleague.
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Tuan P. Tran, Nandini Patel, Brian Samas, Jacob B. Schwarz
(Communication from Org. Biomol. Chem.)
Tuan P. Tran, Org. Biomol. Chem., 2009, DOI: 10.1039/b916188k
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Source: RSC - Organic Biomolecular Chemistry - October 14, 2009 Category: Molecular Biology Authors: Tuan P. TranNandini Patel Brian Samas Jacob B. Schwarz Source Type: journals
Prediction of the main macrocyclic conjugation pathway for porphyrinoids from the ring current distributionEmail this article to a colleague.
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Jun-ichi Aihara, Masakazu Makino
(Paper from Org. Biomol. Chem.)
Jun-ichi Aihara, Org. Biomol. Chem., 2010, DOI: 10.1039/b910521b
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Source: RSC - Organic Biomolecular Chemistry - October 14, 2009 Category: Molecular Biology Authors: Jun-ichi AiharaMasakazu Makino Source Type: journals
Selective demethylation and debenzylation of aryl ethers by magnesium iodide under solvent-free conditions and its application to the total synthesis of natural productsEmail this article to a colleague.
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Kai Bao, Aixue Fan, Yi Dai, Liang Zhang, Weige Zhang, Maosheng Cheng, Xinsheng Yao
(Paper from Org. Biomol. Chem.)
Kai Bao, Org. Biomol. Chem., 2009, DOI: 10.1039/b916969e
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Source: RSC - Organic Biomolecular Chemistry - October 14, 2009 Category: Molecular Biology Authors: Kai BaoAixue Fan Yi Dai Liang Zhang Weige Zhang Maosheng Cheng Xinsheng Yao Source Type: journals
Novel dihydro-[small beta]-agarofuran sesquiterpenes as potent modulators of human P-glycoprotein dependent multidrug resistanceEmail this article to a colleague.
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David Torres-Romero, Francisco Munoz-Martinez, Ignacio A. Jimenez, Santiago Castanys, Francisco Gamarro, Isabel L. Bazzocchi
(Paper from Org. Biomol. Chem.)
David Torres-Romero, Org. Biomol. Chem., 2009, DOI: 10.1039/b915678j
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Source: RSC - Organic Biomolecular Chemistry - October 14, 2009 Category: Molecular Biology Authors: David Torres-RomeroFrancisco Munoz-Martinez Ignacio A. Jimenez Santiago Castanys Francisco Gamarro Isabel L. Bazzocchi Source Type: journals
Towards a chemo-enzymatic method for the asymmetric synthesis of [small beta]-amino tertiary alcoholsEmail this article to a colleague.
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Andrea March-Cortijos, Timothy J. Snape
(Paper from Org. Biomol. Chem.)
Andrea March-Cortijos, Org. Biomol. Chem., 2009, DOI: 10.1039/b916013b
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Source: RSC - Organic Biomolecular Chemistry - October 14, 2009 Category: Molecular Biology Authors: Andrea March-CortijosTimothy J. Snape Source Type: journals
A nanofibrous cell-seeded hydrogel promotes integration in a cartilage gap modelEmail this article to a colleague.
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This study suggests that a cell-seeded TGF[beta]3-supplemented hydrogel can encourage integration between two opposing pieces of articular cartilage. Copyright © 2009 John Wiley & Sons, Ltd.
Source: Journal of Tissue Engineering and Regenerative Medicine - October 14, 2009 Category: Molecular Biology Authors: S. A. Maher, R. L. Mauck, L. Rackwitz, R. S. Tuan Source Type: journals
Human tissue-engineered bone produced in clinically relevant amounts using a semi-automated perfusion bioreactor system: a preliminary studyEmail this article to a colleague.
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The aim of this study was to evaluate a semi-automated perfusion bioreactor system for the production of clinically relevant amounts of human tissue-engineered bone. Human bone marrow stromal cells (hBMSCs) of eight donors were dynamically seeded and proliferated in a perfusion bioreactor system in clinically relevant volumes (10 cm3) of macroporous biphasic calcium phosphate scaffolds (BCP particles, 2-6 mm). Cell load and distribution were shown using methylene blue staining. MTT staining was used to demonstrate viability of the present cells. After 20 days of cultivation, the particles were covered with a homogeneous la...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 14, 2009 Category: Molecular Biology Authors: F. W. Janssen, R. van Dijkhuizen-Radersma, A. Van Oorschot, J. Oostra, J. D. de Bruijn, C. A. Van Blitterswijk Source Type: journals
Curcumin modulates eukaryotic initiation factors in human lung adenocarcinoma epithelial cells.Email this article to a colleague.
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The objective of this study was to exam the curcumin cytotoxic effect and modulation of two major rate-limiting translation initiation factors, including eIF2alpha and eIF4E protein expression levels in lung adenocarcinoma epithelial cell line A549. Cytotoxicity was measured by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and protein changes were determined by Western blot. A549 cells were treated with 0-240 muM curcumin for 4-96 h. The inhibitory effects of curcumin on cytotoxicity were dose- and time-dependent (P < 0.001). The 50% inhibitory curcumin concentrations (IC50s) at 24, 48, 72, an...
Source: Molecular Biology Reports - October 14, 2009 Category: Molecular Biology Authors: Chen L, Tian G, Shao C, Cobos E, Gao W Tags: Mol Biol Rep Source Type: journals
Variability of CD3 membrane expression and T cell activation capacityEmail this article to a colleague.
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In conclusion, T cells have highly heterogeneous CD3 expression, possibly predetermined and with clear functional significance. © 2009 Clinical Cytometry Society
Source: Cytometry Part B: Clinical Cytometry - October 14, 2009 Category: Molecular Biology Authors: Fatima-Zahra El Hentati, Frederic Gruy, Cristina Iobagiu, Claude Lambert Source Type: journals
Cytometric assessment of cytostatic and cytotoxic effects of topical glaucoma medications on human epithelial corneal line cellsEmail this article to a colleague.
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We examined the effect of glaucoma topical medications (bimatoprost, travoprost, latanoprost, timolol, betaxolol, dorzolamide, brinzolamide, brimonidine) on growth of cells of three human epithelial corneal lines.The cells were cultured in 8-chamber slides, treated with different concentrations of the medications, and fixed at 24, 48, and 72 h. Cell number on slides to estimate viability and growth curves, frequency of apoptosis (FLICA and caspase-3 activation probes), and proliferation (BrdU incorporation assay) were measured by laser scanning cytometry (LSC).Depending on concentration all examined medications induced cel...
Source: Cytometry Part B: Clinical Cytometry - October 14, 2009 Category: Molecular Biology Authors: Dorota Pozarowska, Piotr Pozarowski, Zbigniew Darzynkiewicz Source Type: journals
Positive selection for the male functionality of a co-retroposed gene in the hominoidsEmail this article to a colleague.
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Conclusions:
Therefore, this set of analyses showed that PIPSL is an extraordinary co-retroposed protein-coding gene that may participate in the male functions of humans and its close relatives.
Source: BMC Evolutionary Biology - Latest articles - October 14, 2009 Category: Molecular Biology Authors: Yong Edward ZhangShujuan LuShuqi ZhaoXiaofeng ZhengManyuan LongLiping Wei Source Type: journals
Cyanobacteria MT gene SmtA enhance zinc tolerance in Arabidopsis.Email this article to a colleague.
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In this study, we report a functional analysis of SmtA in Arabidopsis thaliana and its response to zinc stress. After high zinc stress, the transgenic plants over-expressing SmtA showed higher survival rate than the wild type. We also found that over-expression of SmtA in Arabidopsis increased the activities of SOD and POD, and enhanced the tolerance to zinc stress. Together, our results indicate that SmtA may play an important role in the response to zinc stress in Arabidopsis.
PMID: 19830591 [PubMed - as supplied by publisher]
Source: Molecular Biology Reports - October 14, 2009 Category: Molecular Biology Authors: Xu J, Tian YS, Peng RH, Xiong AS, Zhu B, Hou XL, Yao QH Tags: Mol Biol Rep Source Type: journals
Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii.Email this article to a colleague.
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In this study, an MnSOD gene (TaMnSOD) from Tamarix androssowii, under the control of the CaMV35S promoter, was introduced into poplar (Populus davidiana x P. bolleana). The physiological parameters, including SOD activity, malondialdehyde (MDA) content, relative electrical conductivity (REC) and relative weight gain, of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that SOD activity was enhanced in transgenic plants, and the MDA content and REC were significantly decreased compared to WT plants when exposed to NaCl stress. In addition, the relative weight gains of the transgeni...
Source: Molecular Biology Reports - October 14, 2009 Category: Molecular Biology Authors: Wang YC, Qu GZ, Li HY, Wu YJ, Wang C, Liu GF, Yang CP Tags: Mol Biol Rep Source Type: journals
Life Began When Evolution Began: A Lipidic Vesicle-Based Scenario.Email this article to a colleague.
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The research on the origin of life, as such, seems to have reached an impasse as a clear and universal scientific definition of life is probably impossible. On the contrary, the research on the origin of evolution may provide a clue. But it is necessary to identify the minimum requirements that allowed evolution to emerge on early Earth. The classical approach, the 'RNA world hypothesis' is one way, but an alternative based on nonlinear dynamics dealing with far-from-equilibrium self-organization and dissipative structures can also be proposed. The conditions on early Earth, near deep-sea hydrothermal sites, were favor...
Source: Origins of Life and Evolution of the Biosphere - October 14, 2009 Category: Molecular Biology Authors: Tessera M Tags: Orig Life Evol Biosph Source Type: journals
Breast Cancer Cell Growth Inhibition by Phenethyl Isothiocyanate is Associated with Downregulation of Estrogen Receptor-alpha36.Email this article to a colleague.
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The dietary isothiocyanates (ITCs) exhibit strong chemopreventive activities for varieties of neoplasms including breast cancer. However, the molecular mechanisms underlying ITC function in breast cancer cells have not been well established. Here, we found that phenethyl isothiocyanate (PEITC) acted more potently than the "pure" antiestrogen ICI 182,780 to inhibit the growth of estrogen receptor (ER)-positive breast cancer MCF7 and H3396 cells and ER-negative MDA-MB-231 and SK-BR-3 cells. PEITC reduced the steady state levels of ER-alpha and it's novel variant, ER-alpha36 in a dose-and time-dependent manner and inhibit...
Source: J Cell Mol Med - October 14, 2009 Category: Molecular Biology Authors: Kang L, Wang ZY Tags: J Cell Mol Med Source Type: journals
Synthesis of novel synthetic intermediates from the reaction of benzimidazole and triazole carbenes with ketenimines and their application in the construction of spiro-pyrrolesEmail this article to a colleague.
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Jun-Ming Mo, Yang-Guang Ma, Ying Cheng
(Paper from Org. Biomol. Chem.)
Jun-Ming Mo, Org. Biomol. Chem., 2009, DOI: 10.1039/b915215f
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Source: RSC - Organic Biomolecular Chemistry - October 13, 2009 Category: Molecular Biology Authors: Jun-Ming MoYang-Guang Ma Ying Cheng Source Type: journals
Rapid synthesis of Abelson tyrosine kinase inhibitors using click chemistryEmail this article to a colleague.
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Karunakaran A. Kalesh, Kai Liu, Shao Q. Yao
(Paper from Org. Biomol. Chem.)
Karunakaran A. Kalesh, Org. Biomol. Chem., 2009, DOI: 10.1039/b913333j
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Source: RSC - Organic Biomolecular Chemistry - October 13, 2009 Category: Molecular Biology Authors: Karunakaran A. KaleshKai Liu Shao Q. Yao Source Type: journals
Polyploid evolution in Oryza officinalis complex of the genus OryzaEmail this article to a colleague.
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C-genome, shared by all polyploid species in the complex, had experienced different evolutionary history particularly after polyploidization, e.g., inter-genomic exchange in BBCC and genomic invasion in CCDD tetraploids. It diverged from B-genome at 4.8 Mya, then participated the tetraploid formation span from 0.9 to 0.3 Mya, and spread into tropics of the disjunct continents by transcontinentally long-distance dispersal, instead of vicariance, as proposed by this study, given that the continental splitting was much earlier than the C-genome species radiation. We also find reliable evidence indicated that an e...
Source: BMC Evolutionary Biology - Latest articles - October 13, 2009 Category: Molecular Biology Authors: Baosheng WangZhuoya DingWei LiuJin PanChangbao LiSong GeDaming Zhang Source Type: journals
Tripod amphiphiles for membrane protein manipulationEmail this article to a colleague.
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Pil Seok Chae, Philip D. Laible, Samuel H. Gellman
(Review from Mol. BioSyst.)
Pil Seok Chae, Mol. BioSyst., 2010, DOI: 10.1039/b915162c
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Source: RSC - Mol. BioSyst. latest articles - October 13, 2009 Category: Molecular Biology Authors: Pil Seok ChaePhilip D. Laible Samuel H. Gellman Source Type: journals
Cooperative social clusters are not destroyed by dispersal in a ciliateEmail this article to a colleague.
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High dispersal among aggregative strains may not destroy group stability in T. thermophila because the dispersal polymorphism allows social strains to more readily escape kin groups than less aggregative strains, yet still benefit from stable group membership among sedentary morphs. Such dispersal polymorphisms should be common in other social organisms, serving to alter the nature of the negative impact of dispersal on social evolution.
Source: BMC Evolutionary Biology - Latest articles - October 13, 2009 Category: Molecular Biology Authors: Nicolas SchtickzelleElse FjerdingstadAlexis ChaineJean Clobert Source Type: journals
In situ adipogenesis in fat tissue augmented by collagen scaffold with gelatin microspheres containing basic fibroblast growth factorEmail this article to a colleague.
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In situ adipose tissue regeneration in fat tissue by collagen sponges and gelatin microspheres containing basic fibroblast growth factor (bFGF) was investigated. A minced collagen sponge scaffold (1 ml) was incorporated with microspheres containing 10 µg bFGF and administered into a defect of rabbit fat tissues. Adipogenesis at the administered site was evaluated histologically. The adipose tissue regeneration induced by the administration of mixed collagen scaffold and microspheres containing bFGF was significantly stronger than that of either collagen scaffold alone or microspheres containing bFGF alone. The histologica...
Source: Journal of Tissue Engineering and Regenerative Medicine - October 13, 2009 Category: Molecular Biology Authors: Yu Kimura, Wakako Tsuji, Hiroyasu Yamashiro, Masakazu Toi, Takashi Inamoto, Yasuhiko Tabata Source Type: journals
