Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 15.
The potential of microfluidic water-in-oil droplets in experimental biology
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Yolanda Schaerli, Florian Hollfelder
(Review from Mol. BioSyst.)
Yolanda Schaerli, Mol. BioSyst., 2009, DOI: 10.1039/b907578j
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The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - October 11, 2009 Category: Molecular Biology Authors: Yolanda SchaerliFlorian Hollfelder Source Type: journals
Component match in rhodium catalyzed three-component reactions of ethyl diazoacetate, H2O and aryl imines: a highly diastereoselective one-step synthesis of [small beta]-aryl isoserine derivativesEmail this article to a colleague.
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Zhenqiu Guo, Taoda Shi, Jun Jiang, Liping Yang, Wenhao Hu
(Paper from Org. Biomol. Chem.)
Zhenqiu Guo, Org. Biomol. Chem., 2009, DOI: 10.1039/b915013g
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The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 11, 2009 Category: Molecular Biology Authors: Zhenqiu GuoTaoda Shi Jun Jiang Liping Yang Wenhao Hu Source Type: journals
Novel asymmetrically functionalized bis-dipicolylamine metal complexes: peripheral decoration of a potent anion recognition scaffoldEmail this article to a colleague.
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Joel A. Drewry, Steven Fletcher, Haider Hassan, Patrick T. Gunning
(Paper from Org. Biomol. Chem.)
Joel A. Drewry, Org. Biomol. Chem., 2009, DOI: 10.1039/b917692f
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Source: RSC - Organic Biomolecular Chemistry - October 11, 2009 Category: Molecular Biology Authors: Joel A. DrewrySteven Fletcher Haider Hassan Patrick T. Gunning Source Type: journals
Werner syndrome gene variants in human sarcomasEmail this article to a colleague.
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Werner syndrome is an autosomal inherited disease that is characterized by premature aging. The gene mutated in Werner syndrome (WS), WRN, encodes both a 3[prime] [rarr] 5[prime] DNA helicase and a 3[prime] [rarr] 5[prime] DNA exonuclease. Among the WS phenotypes is an exceptionally high incidence of sarcomas. We asked whether spontaneous sarcomas, not known to be associated with WS, also harbor mutations or unreported single nucleotide polymorphisms (SNPs) in WRN. We analyzed RNA or DNA sequences within the helicase and exonuclease domains from 51 and 69 matched sarcoma and adjacent normal tissues, respectively. Among a t...
Source: Molecular Carcinogenesis - October 11, 2009 Category: Molecular Biology Authors: Jessica J. Hsu, Ashwini S. Kamath-Loeb, Eitan Glick, Brett Wallden, Karen Swisshelm, Brian P. Rubin, Lawrence A. Loeb Source Type: journals
Cyto-nuclear discordance in the phylogeny of Ficus section Galoglychia and host shifts in plant-pollinator associationsEmail this article to a colleague.
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Conclusions:
As hypothesized, we discovered evidences of past hybridization in Ficus section Galoglychia. Further, introgression was only detected in subsections presenting incongruence between plant and pollinator phylogenies and taxonomy. This supports the hypothesis that host shift is the cause for plant-pollinator incongruence. Moreover, small fig size could facilitate host shifts. Eventually, this study demonstrates that non-coding chloroplast markers are valuable to resolve deep nodes in Ficus phylogeny.
Source: BMC Evolutionary Biology - Latest articles - October 11, 2009 Category: Molecular Biology Authors: Julien RenoultFinn KjellbergCinderella GroutSylvain SantoniBouchaib Khadari Source Type: journals
Emerging biology of vascular wall progenitor cells in health and disease.Email this article to a colleague.
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New blood vessels are formed through angiogenesis and postnatal vasculogenesis. Thus, it is essential to identify vascular stem and progenitor cell niches and the mechanisms governing their role in blood vessel formation. Although much is known about circulating and bone marrow-derived endothelial progenitor cells (EPCs), little is known about the vascular wall as an EPC niche. Experimental evidence strongly suggests that EPCs, as well as other stem and progenitor cells, reside in distinct zones of the vessel wall, such as within the subendothelial space and in the so-called "vasculogenic zone" within the vascular adve...
Source: Trends in Molecular Medicine - October 11, 2009 Category: Molecular Biology Authors: Tilki D, Hohn HP, Ergün B, Rafii S, Ergün S Tags: Trends Mol Med Source Type: journals
Fetal-maternal exchange of multipotent stem/progenitor cells: microchimerism in diagnosis and disease.Email this article to a colleague.
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The biological concept of microchimerism, the bidirectional trafficking and stable long-term persistence of small numbers of allogeneic (fetal and maternal) cells in a genetically different organ, has gained considerable attention. Microchimerism is a common phenomenon in many species, including humans, and microchimeric cells can modify immunological recognition or tolerance, affect the course and outcome of various diseases and demonstrate stem cell-like or regenerative potential. Here, we review current knowledge of the biology of microchimerism and show how long-term allogeneic co-existence within an organism can i...
Source: Trends in Molecular Medicine - October 11, 2009 Category: Molecular Biology Authors: Klonisch T, Drouin R Tags: Trends Mol Med Source Type: journals
Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity.Email this article to a colleague.
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A small HSP gene, ZmsHSP, was isolated from Zea mays. Sequence analysis revealed that the open reading frame of ZmsHSP was 477 bp and that it encodes a protein composed of 159 amino acid residues with a calculated molecular mass of 18.17 kD and a predicated isoelectric point (pI) of 5.63. ZmsHSP contains a CS domain (p23-like domain) and shares similarity with the HSP90 co-chaperone p23. The expression level of ZmsHSP was different among various tissues with the highest expression in leaves and the lowest in silks. Results also showed that the expression of ZmsHSP in maize was significantly up-regulated by dehydration....
Source: Molecular Biology Reports - October 10, 2009 Category: Molecular Biology Authors: Cao Z, Jia Z, Liu Y, Wang M, Zhao J, Zheng J, Wang G Tags: Mol Biol Rep Source Type: journals
Plk1 phosphorylation of Topors is involved in its degradation.Email this article to a colleague.
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Topors is a DNA topoisomerase I- and p53-binding protein, and mainly functions as a p53 regulator. Accumulating evidence also supports the notion that Topors plays the role as a negative regulator of cell growth, and possibly as a tumor suppressor. Here, we demonstrated that Topors is also involved in normal mitotic progression, since Topors depletion delays mitotic entry and affects mitotic progression. Furthermore, Topors is degradated in response to the activation of the spindle checkpoint. Significantly, Polo-like kinase 1 (Plk1)-associated phosphorylation of Topors at S718 is essential for nocodazole-induced degra...
Source: Molecular Biology Reports - October 10, 2009 Category: Molecular Biology Authors: Yang X, Li H, Deng A, Liu X Tags: Mol Biol Rep Source Type: journals
Evidence for a new allele at the SERCA1 locus affecting pork meat quality in part through the imbalance of Ca(2+) homeostasis.Email this article to a colleague.
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In this study, a novel single nucleotide polymorphism (SNP) in exon 8 (C > T) was detected by tetra-primer ARMS-PCR and the tissue expression pattern of SERCA1 was analyzed in eleven tissues. A model of primary skeletal muscle cells in vitro exposed to dexamethasone (DEX, a synthetic corticosteroid) was also employed to determine whether stress hormones cause an increase in intracellular Ca(2+) concentration that is associated with alteration in SERCA1 and in turn subsequently affect meat quality. The results showed that the CC genotype has lower content intramuscular fat and higher water than pig carrying the genotype ...
Source: Molecular Biology Reports - October 10, 2009 Category: Molecular Biology Authors: Chai J, Xiong Q, Zhang PP, Shang YY, Zheng R, Peng J, Jiang SW Tags: Mol Biol Rep Source Type: journals
Cardiac matrix remodeling following intracoronary cell transplantation in dilated cardiomyopathic rabbits.Email this article to a colleague.
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Cellular cardiomyoplasty has been proposed as a promising therapeutic strategy for chronic heart failure. Previous studies focused on structural changes in cardiomyocytes to explain the potential benefits for contractile function. However, limited information is available about the cardiac matrix remodeling following cell transplantation in dilated cardiomyopathy (DCM). Here, we established a new animal model of intracoronary bone marrow mononuclear cells (BMMNCs) transplantation to explore extracellular matrix remodeling in adriamycin-induced cardiomyopathic rabbits. In vivo studies demonstrated that BMMNCs transplant...
Source: Molecular Biology Reports - October 10, 2009 Category: Molecular Biology Authors: Jin B, Luo XP, Ni HC, Li Y, Shi HM Tags: Mol Biol Rep Source Type: journals
Isolation and functional characterization of DgZFP: a gene encoding a Cys(2)/His (2)-type zinc finger protein in chrysanthemum.Email this article to a colleague.
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A Cys(2)/His(2)-type zinc finger protein gene, DgZFP, was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE) approach. The DgZFP encodes a protein of 211 amino acids residues with a calculated molecular mass of 22.9 kDa and theoretical isoelectric point is 8.59. DgZFP contains two Cys(2)/His(2)-type zinc finger motifs, one nuclear localization domain, one Leu-rich domain, and one ethylene-responsive element-binding factor (ERF)-associated amphiphilic repression (EAR) domain. The transcript of DgZFP was enriched in flowers than in roots, stems, and leaves of the adult chrysanthemum plants. The gene e...
Source: Molecular Biology Reports - October 10, 2009 Category: Molecular Biology Authors: Liu QL, Xu KD, Ma N, Zeng L, Zhao LJ Tags: Mol Biol Rep Source Type: journals
Knockdown of the prion gene expression by RNA interference in bovine fibroblast cells.Email this article to a colleague.
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PRNP is the gene encoding prion protein whose misfolded and beta-sheet-rich isoform is the infectious agent of transmissible spongiform encephalopathy (TSE). TSE, also called prion diseases, cause fatal neurodegenerative and transmissible disorders in human and animals. Among these diseases, bovine spongiform encephalopathy (BSE) has tremendous impact on economy and human health in the world. In the present study, we hypothesize suppression of the PRNP gene expression could raise resistance to BSE in cattle by using vector-based small interfering RNA (siRNA) expression systems. Therefore, the objective was to screen ef...
Source: Molecular Biology Reports - October 10, 2009 Category: Molecular Biology Authors: Wang S, Lv X, Zhang K, Lin T, Liu X, Yuan J, Dai Y, Li N Tags: Mol Biol Rep Source Type: journals
The complete mitochondrial genomes of the whistling duck (Dendrocygna javanica) and black swan (Cygnus atratus): dating evolutionary divergence in Galloanserae.Email this article to a colleague.
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In this study, we present two additional mitochondrial genome sequences of Galloanserae (the whistling duck, Dendrocygna javanica, and the black swan, Cygnus atratus) to broaden the scope of molecular phylogenetic reconstruction. The lengths of the whistling duck's and black swan's mitochondrial genomes are 16,753 and 16,748 bases, respectively. Phylogenetic analyses suggest that Dendrocygna is more likely to be in a basal position of the branch consisting of Anatinae and Anserinae, an affiliation that does not conform to its traditional classification. Bayesian approaches were employed to provide a rough timescale for Gal...
Source: Molecular Biology Reports - October 10, 2009 Category: Molecular Biology Authors: Jiang F, Miao Y, Liang W, Ye H, Liu H, Liu B Tags: Mol Biol Rep Source Type: journals
Role of syndecan-3 polymorphisms in obesity and female hyperandrogenismEmail this article to a colleague.
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Abstract The heparan sulfate proteoglycan syndecan-3 (SDC3) is a novel regulator of feeding behavior and body weight. Recently, an association of SDC3 polymorphisms with obesity has been observed in Koreans. As female obesity is associated with hyperandrogenism and infertility,
we studied the role of SDC3 polymorphisms in female individuals undergoing diagnostics prior to infertility treatment. For this purpose, endocrine parameters
and body mass index of 249 women were assessed. Genotyping of V208I, D303N, and T329I was performed with TaqMan technology
using lymphocyte-derived DNA and allelic discrimination ...
Source: Journal of Molecular Medicine - October 10, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
NBDT (3-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-3-toluene) - A novel fluorescent dye for studying mechanisms of toluene uptake into vital bacteriaEmail this article to a colleague.
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In this study, a fluorescently labeled toluene analogue dye (3-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-3-toluene; NBDT), flow cytometry, and shot gun proteome analysis were used to follow toluene uptake into bacteria in more detail. The new dye has excitation peaks at 444 and 475 nm and an emission peak at 537 nm. The toluene-degraders P. putida mt-2 and P. putida F1 as well as P. putida KT2440 and E. coli K12 as negative controls were included. To enable quantification of NBDT uptake, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) was added to inactivate NBDT efflux pumps. The porin inhibitor cadaverine was added to...
Source: Cytometry Part A - October 9, 2009 Category: Molecular Biology Authors: H. Sträuber, T. Hübschmann, N. Jehmlich, F. Schmidt, M. von Bergen, H. Harms, S. Müller Source Type: journals
Association of the SDF1-3'A polymorphism with susceptibility to myocardial infarction in Chinese Han population.Email this article to a colleague.
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This study was aimed to investigate whether the SDF1-3'A polymorphism (rs1801157) is associated to myocardial infarction (MI) in a sample of Chinese Han population. A total of 560 patients with MI and 532 controls were enrolled in the study. The SDF1-3'A polymorphism was determined by polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) analysis. A significant difference in genotype distribution and allele frequency was observed between patients and controls (P = 0.003 and P = 0.001, respectively). The A allele carriers had a significantly reduced MI risk compared with the GG homozygotes (OR, 0.69...
Source: Molecular Biology Reports - October 9, 2009 Category: Molecular Biology Authors: Luan B, Han Y, Zhang X, Kang J, Yan C Tags: Mol Biol Rep Source Type: journals
cDNA cloning and expression of ghrelin in giant panda (Ailuropoda melanoleuca).Email this article to a colleague.
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Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor. It plays an important role in stimulating growth hormone secretion, food intake, body weight gain and gastric motility. cDNA sequences coding for ghrelin precursor protein (prepro-ghrelin) were isolated from the stomach of a giant panda. Two different mRNA sequences of ghrelin were obtained. The long open reading frame of ghrelin (354 bp) encodes a precursor protein of 117 amino acids with a 23 amino acid signal peptide. The short one (351 bp) encodes a precursor protein of 116 amino acids with the same 23 amino acid signal peptide. The presu...
Source: Molecular Biology Reports - October 9, 2009 Category: Molecular Biology Authors: Xu Q, Bian H, Han N, Hou R, Zhang Z, Zhu M Tags: Mol Biol Rep Source Type: journals
MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and promotes cell proliferation.Email this article to a colleague.
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To identify the possible microRNAs (miRNAs) which target the polycystic kidney disease-2 gene (PKD2), and clarify effects of the miRNAs on PKD2. We preliminarily used bioinformatics to analyze 3'UTR (3'untranslated regions) of PKD1 and PKD2 in order to predict the potential microRNAs targeted on them. Subsequently, the stable cell lines with overexpression of microRNA-17 (miR-17) were screened, and luciferase assay combined with the mutation 3'UTR of PKD2 were performed to verify PKD2 is the target of miR-17. Moreover, RT-PCR and Western Blotting were used to determine the post-transcriptionally regulation of PKD2 by m...
Source: Molecular Biology Reports - October 9, 2009 Category: Molecular Biology Authors: Sun H, Li QW, Lv XY, Ai JZ, Yang QT, Duan JJ, Bian GH, Xiao Y, Wang YD, Zhang Z, Liu YH, Tan RZ, Yang Y, Wei YQ, Zhou Q Tags: Mol Biol Rep Source Type: journals
MicroRNAs take part in pathophysiology and pathogenesis of Male Pattern Baldness.Email this article to a colleague.
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Male Pattern Baldness (MPB) or androgenetic alopecia is a common form of hair loss with androgens and genetics having etiological significance. Androgens are thought to pathophysiologically power on cascades of chronically dramatic alterations in genetically susceptible scalp dermal papillas, specialized cells in hair follicles in which androgens react, and finally resulting in a patterned alopecia. However, the exact mechanisms through which androgens, positive regulators of growth and anabolism in most body sites, paradoxically exert their effects on balding hair follicles, are not yet known. The role of microRNAs, a...
Source: Molecular Biology Reports - October 9, 2009 Category: Molecular Biology Authors: Goodarzi HR, Abbasi A, Saffari M, Tabei MB, Noori Daloii MR Tags: Mol Biol Rep Source Type: journals
The study of ginsenoside on PPARgamma expression of mononuclear macrophage in type 2 diabetes.Email this article to a colleague.
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Peroxisome proliferator-activated receptors (PPARs) are transcriptional factors, which play a key role in modulating glucose and lipid metabolism and in the pathogenesis of atherosclerosis. Ginsenosides are the active components of ginseng, which is a perennial aromatic herb that is widely used in China for medicinal purposes. Some studies have reported that ginsenosides have anti-hyperglycemia and anti-obesity effects that involve the PPAR-mediated pathway. The aims of this study were to investigate mononuclear macrophage PPARgamma mRNA expression of type 2 diabetes and the effect of ginsenosides on PPARgamma mRNA exp...
Source: Molecular Biology Reports - October 9, 2009 Category: Molecular Biology Authors: Ni HX, Yu NJ, Yang XH Tags: Mol Biol Rep Source Type: journals
Role of aldose reductase in the high glucose induced expression of fibronectin in human mesangial cells.Email this article to a colleague.
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Aldose reductase (AR) has emerged as a key contributor to the diabetic nephropathy (DN), however, the mechanisms by which AR increases DN remain poorly understood. Here, we report that exposure to high glucose (HG) stimulates fibronectin (FN) from human mesangial cells in culture. Our results show that exposure to HG and overexpression AR increase the expression of FN. This increase was prevented by the AR inhibitors sorbinil and zopolrestat. Treatment with HG and transfected with plasmid PcDNA3.0-AR, resulted in phosphorylation and activation of ERK, JNK and AKT signaling pathway, and increase the expression of FN. Tr...
Source: Molecular Biology Reports - October 9, 2009 Category: Molecular Biology Authors: Huang P, Zhang Y, Jiang T, Zeng W, Zhang N Tags: Mol Biol Rep Source Type: journals
Identification of genes involved in stem rust resistance from wheat mutant D51 with the cDNA-AFLP technique.Email this article to a colleague.
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In this study, we used the cDNA-AFLP technology to identify the genes that are likely involved in the stem rust resistance. EcoRI/MseI selective primers were used to generate approximately 1920 DNA fragments. Seventy five differentially transcribed fragments (3.91%) were identified by comparing the samples of 21C3CPH infected D51 with infected L6239 or uninfected D51. Eleven amplified cDNA fragments were sequenced. Eight showed significant similarity to known genes, including TaLr1 (leaf rust resistance gene), wlm24 (wheat powdery mildew resistance gene), stress response genes and ESTs of environment stress of tall fescue....
Source: Molecular Biology Reports - October 9, 2009 Category: Molecular Biology Authors: Yin J, Wang G, Xiao J, Ma F, Zhang H, Sun Y, Diao Y, Huang J, Guo Q, Liu D Tags: Mol Biol Rep Source Type: journals
CXCL10 expression and prognostic significance in stage II and III colorectal cancer.Email this article to a colleague.
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In this study, we focus on the expression of CXCL10 in stage II and III colorectal cancer (CRC) and its correlation with metastasis. Real-time reverse-transcription polymerase chain reaction (real-time RT-PCR) and RT-PCR were performed in 36 snap-frozen CRC tissues with recurrence and 28 CRC tissues without recurrence to examine CXCL10 expression. For further examination in mRNA level, three CRC-metastasis-derived cell lines (SW620, Lovo, Colo205) and three primary-CRC-derived ones (SW480, Caco-2, HCT116) were also subjected to real-time RT-PCR. Analysis showed that CXCL10 down-regulated in CRC with recurrence both in tiss...
Source: Molecular Biology Reports - October 9, 2009 Category: Molecular Biology Authors: Jiang Z, Xu Y, Cai S Tags: Mol Biol Rep Source Type: journals
Characterization of the expression of HTm4 (MS4A3), a cell cycle regulator, in human peripheral blood cells and normal and malignant tissues.Email this article to a colleague.
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Abstract HTm4 (MS4A3) is a member of a family of four-transmembrane proteins designated MS4A. MS4A proteins fulfill diverse functions, acting as cell surface signaling molecules and intracellular adapter proteins. Early reports demonstrated that HTm4 is largely restricted to the hematopoietic lineage, and is involved in cell cycle control, via a regulatory interaction with the KAP phosphatase, Cyclin A and CDK2. Here we describe the expression pattern of HTm4 in peripheral blood cells using gene expression microarray technology, and in normal fetal and adult human tissues, as well as adult human cancers, using tissue m...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Kutok JL, Yang X, Folkerth R, Adra CN Tags: J Cell Mol Med Source Type: journals
Zoledronic acid repolarizes tumor-associated macrophages and inhibits mammary carcinogenesis by targeting the mevalonate pathway.Email this article to a colleague.
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ABSTRACT It is unknown whether zoledronic acid at clinically relevant doses is active against tumors not located in bone. Mice transgenic for the activated ErbB-2 oncogene were treated with a cumulative number of doses equivalent to that recommended in humans. A significant increase in tumor-free and overall survival was observed in mice treated with zoledronic acid. At clinically compatible concentrations, zoledronic acid modulated the mevalonate pathway and affected protein prenylation in both tumor cells and macrophages. A marked reduction in the number of tumor associated macrophages was paralleled by a significant...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Coscia M, Quaglino E, Iezzi M, Curcio C, Pantaleoni F, Riganti C, Holen I, Mönkkönen H, Boccadoro M, Forni G, Musiani P, Bosia A, Cavallo F, Massaia M Tags: J Cell Mol Med Source Type: journals
Epigenetic modulation of the protein kinase a RIIalpha (PRKAR2A) gene by histone deacetylases 1 & 2 in human smooth muscle cells.Email this article to a colleague.
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Epigenetic modulation of the protein kinase a RIIalpha (PRKAR2A) gene by histone deacetylases 1 & 2 in human smooth muscle cells.
J Cell Mol Med. 2009 Oct 10;
Authors: Karolczak-Bayatti M, Loughney AD, Robson SC, Europe-Finner GN
Abstract Recently we reported that the expression of the PKA regulatory subunit RIIalpha is dynamically regulated in human smooth muscle cells of the uterus. We showed that expression levels of mRNA/protein were substantially increased during pregnancy and decreased upon labour, changes that were mirrored by particulate type II PKA activity. This implied an important role for RIIal...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Karolczak-Bayatti M, Loughney AD, Robson SC, Europe-Finner GN Tags: J Cell Mol Med Source Type: journals
Platelet microparticle levels: A biomarker of thromboangiitis obliterans (Buerger's disease) exacerbation.Email this article to a colleague.
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PMID: 19818096 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Darnige L, Helley D, Fischer AM, Emmerich J, Smadja DM, Fiessinger JN Tags: J Cell Mol Med Source Type: journals
Mechanisms of intermittent hypoxia-induced hypertension.Email this article to a colleague.
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Exposing rodents to brief episodes of hypoxia mimics the hypoxemia and the cardiovascular and metabolic effects observed in patients with obstructive sleep apnea (OSA), a condition that affects between 5% to 20% of the population. Apart from daytime sleepiness, OSA is associated with a high incidence of systemic and pulmonary hypertension, peripheral vascular disease, stroke and sudden cardiac death. The development of animal models to study sleep apnea has provided convincing evidence that recurrent exposure to intermittent hypoxia has significant vascular and hemodynamic impacts that explain much of the cardiovascula...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: González Bosc LV, Resta T, Walker B, Kanagy NL Tags: J Cell Mol Med Source Type: journals
Transport characteristics of a novel peptide platform for CNS therapeutics.Email this article to a colleague.
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ABSTRACT New and effective therapeutics that cross the blood-brain barrier (BBB) are critically needed for treatment of many brain diseases. We characterize here a novel drug development platform that is broadly applicable for the development of new therapeutics with increased brain penetration. The platform is based on the Angiopep-2 peptide, a sequence derived from ligands that bind to low-density lipoprotein receptor-related protein-1 (LRP-1), a receptor expressed on the BBB. Fluorescent imaging studies of a Cy5.5Angiopep-2 conjugate and immunohistochemical studies of injected Angiopep-2 in mice demonstrated efficie...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Bertrand Y, Currie JC, Demeule M, Régina A, Ché C, Abulrob A, Fatehi D, Sartelet H, Gabathuler R, Castaigne JP, Stanimirovic D, Béliveau R Tags: J Cell Mol Med Source Type: journals
A link between the accumulation of DNA damage and loss of multipotency of human mesenchymal stromal cells.Email this article to a colleague.
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Abstract Human mesenchymal stromal cells (hMSCs) represent and attractive cell source for clinic applications. Besides being mutipotent, recent clinical trials suggest that they secrete both trophic and immunomodulatory factors, allowing allogenic MSCs to be used in a wider variety of clinical situations. The yield of prospective isolation is however very low, making expansion a required step towards clinical applications. Unfortunately, this leads to a significant decrease in their stemness. To identify the mechanism behind loss of multipotency, hMSCs were expanded until replicative senescence and the concomitant mole...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Alves H, Munoz-Najar U, de Wit J, Renard AJ, Hoeijmakers JH, Sedivy JM, van Blitterswijk C, de Boer J Tags: J Cell Mol Med Source Type: journals
BiP, an endoplasmic reticulum chaperone, modulates the development of morphine antinociceptive tolerance.Email this article to a colleague.
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We examined knock-in mice expressing a mutant BiP with the retrieval sequence deleted in order to elucidate physiological processes that are sensitive to BiP functions. We tested the thermal antinociceptive effect of morphine in heterozygous mutant BiP mice in a hot plate test. Paw withdrawal latencies before and after a single administration of morphine were not significantly different between the wild-type and mutant BiP mice. Repeated morphine administration caused the development of morphine tolerance in the wild-type mice. The activation of glycogen synthase kinase 3beta (GSK3beta) was associated with morphine toleran...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Dobashi T, Tanabe S, Jin H, Mimura N, Yamamoto T, Nishino T, Aoe T Tags: J Cell Mol Med Source Type: journals
Effect of irreversibly glycated LDL in human vascular smooth muscle cells: Lipid loading, oxidative and inflammatory stress.Email this article to a colleague.
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In conclusion, AGE-LDL activates hSMC (increasing CD36, LRP1, RAGE), inducing a pro-oxidant state (activation of NADPHox), lipid accumulation and a pro-inflammatory state (expression of MCP-1). These results may partly explain the contribution of AGE-LDL and hSMC to the accelerated atherosclerosis in diabetes.
PMID: 19818091 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Sima AV, Botez GM, Stancu CS, Manea A, Raicu M, Simionescu M Tags: J Cell Mol Med Source Type: journals
Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumor protection.Email this article to a colleague.
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s O, Montes-de-Oca-Luna R
ABSTRACT The endoplasmic reticulum (ER) is where the Major Histocompatibility Complex (MHC) Class I molecules are loaded with epitopes to cause an immune cellular response. Most of the protein antigens are degraded in the cytoplasm to amino acids and few epitopes reach the ER. Antigen targeting of this organelle by Calreticulin fusion avoids this degradation and enhances the immune response. We constructed a recombinant adenovirus to express the E7 antigen with an ER-targeting signal peptide (SP) plus an ER retention signal (KDEL sequence). In cell-culture experiments we demonstrated that this...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Loera-Arias MJ, Martínez-Pérez AG, Barrera-Hernández A, Ibarra-Obregón ER, González-Saldívar G, Martínez-Ortega JI, Rosas-Taraco A, Villanueva-Olivo A, Esparza-González SC, Villatoro-Hernandez J, Saucedo-Cárden Tags: J Cell Mol Med Source Type: journals
p53 and the regulation of hepatocyte apoptosis: implications for disease pathogenesis.Email this article to a colleague.
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The interplay between p53 and apoptosis in diseases such as cancer, neurodegeneration, ischemia and atherosclerosis underscores the need to understand the complexity of p53 networks. Here, we highlight recent studies of p53-induced apoptosis in human diseases, with a focus on the modulation of liver cell apoptosis. In addition, recent work has provided new insights into mechanisms underlying the antiapoptotic functions of the endogenous bile acid ursodeoxycholic acid (UDCA), suggesting that the finely tuned, complex control of p53 by Mdm2 is a key step in the UDCA modulation of deregulated, p53-triggered apoptosis. The...
Source: Trends in Molecular Medicine - October 9, 2009 Category: Molecular Biology Authors: Amaral JD, Castro RE, Steer CJ, Rodrigues CM Tags: Trends Mol Med Source Type: journals
Oxidative mechanisms of IL-4-induced IL-6 expression in vascular endothelium.Email this article to a colleague.
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The present study is designed to investigate the effects of interleukin-4 (IL-4) on expression of interleukin-6 (IL-6), as well as to examine the role of distinct sources of reactive oxygen species (ROS) in this process. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) showed that IL-4 significantly up-regulated the mRNA and protein expression of IL-6 in human aortic endothelial cells (HAEC) and C57BL/6 mice. Dihydroethidium (DHE) and dichlorofluorescein (DCF) fluorescence staining demonstrated that IL-4 significantly increased ROS generation in HAEC. A si...
Source: Cytokine - October 9, 2009 Category: Molecular Biology Authors: Lee YW, Lee WH, Kim PH Tags: Cytokine Source Type: journals
Interleukin-1 receptor antagonist gene (IL1RN) polymorphism possibly associated to severity of rheumatic carditis in a Brazilian cohort.Email this article to a colleague.
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Conclusion: In the Brazilian population, the polymorphism of the IL-1ra gene is a relevant factor for rheumatic heart disease severity.
PMID: 19822442 [PubMed - as supplied by publisher]
Source: Cytokine - October 9, 2009 Category: Molecular Biology Authors: Azevedo PM, Bauer R, Caparbo VD, Silva CA, Bonfá E, Pereira RM Tags: Cytokine Source Type: journals
Diagnostic Performance of FDG-PET, MRI, and Plain Film Radiography (PFR) for the Diagnosis of Osteomyelitis in the Diabetic FootEmail this article to a colleague.
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Conclusion FDG-PET is a highly specific imaging modality for the diagnosis of osteomyelitis in diabetic foot and, therefore, should be
considered to be a useful complimentary imaging modality with MRI. In the setting where MRI is contraindicated, the high sensitivity
and specificity of FDG-PET justifies its use after a negative or inconclusive PFR to aid an accurate diagnosis.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0268-2Authors
Asad Nawaz, Department of Radiology, University of Pennsylvania School of Medicine, Hospital of the University of Pennsylvania Division of...
Source: Molecular Imaging and Biology - October 9, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
Therapeutic targeting of signaling pathways in muscular dystrophyEmail this article to a colleague.
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Abstract Muscular dystrophy refers to a group of genetic diseases that cause severe muscle weakness and loss of skeletal muscle mass.
Although research has helped understanding the molecular basis of muscular dystrophy, there is still no cure for this devastating
disorder. Numerous lines of investigation suggest that the primary deficiency of specific proteins causes aberrant activation
of several cell signaling pathways in skeletal and cardiac muscle leading to the pathogenesis of muscular dystrophy. Studies
using genetic mouse models and pharmacological approaches have provided strong evidence that the mod...
Source: Journal of Molecular Medicine - October 9, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Cancer stem cells—clinical relevanceEmail this article to a colleague.
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Abstract Therapeutic advances over the past three decades now allow most cancer patients to achieve major clinical responses. Although
clinical responses can clearly decrease side effects and improve quality of life, most cancer patients still eventually relapse
and die of their disease. Many cancers have now been shown to harbor cells that are phenotypically and biologically similar
to normal cells with self-renewal capacity; these so-called cancer stem cells (CSC) typically constitute only a small fraction
of the total tumor burden, but theoretically harbor all the self-renewal capacity. Moreover, the CSC ...
Source: Journal of Molecular Medicine - October 9, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum.Email this article to a colleague.
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To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA...
Source: Molecular Biology Reports - October 8, 2009 Category: Molecular Biology Authors: Moyano EM, González LM, Cuevas L, Perez-Pastrana E, Santa-Maria Y, Benito A Tags: Mol Biol Rep Source Type: journals
Development of a targeted siRNA delivery system using FOL-PEG-PEI conjugate.Email this article to a colleague.
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Receptor mediated delivery of siRNA enables silencing of target genes in specific tissues. Folate receptor (FR) is an attractive target for tumor-selective gene delivery. The focus of this study was to deliver the dihydrofolate reductase (DHFR) siRNA expressing plasmid and to silence the DHFR gene in FR positive KB cells, by complexing the plasmid with a folate-polyethylene glycol-polyethylenimine (FOL-PEG-PEI) conjugate, as a gene carrier. A DHFR siRNA sequence was cloned into a pSUPER-RNAi vector and complexed with the FOL-PEG-PEI conjugate. The complex was characterized by particle size analyzer, gel retardation and...
Source: Molecular Biology Reports - October 8, 2009 Category: Molecular Biology Authors: Biswal BK, Debata NB, Verma RS Tags: Mol Biol Rep Source Type: journals
Molecular and biochemical analysis of the beta class carbonic anhydrases in Caenorhabditis elegans.Email this article to a colleague.
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The beta class of the carbonic anhydrase (CA) enzyme family has been found in plants, yeast, bacteria and algae, but not in animals. Also, little is known concerning the CAs of C. elegans. Genes possibly encoding beta-CAs were revealed by in silico analysis of the C. elegans genome. Amino acid sequence and 3D structure analysis revealed a resemblance to both plant and cab-type beta-CAs. Temporal expression patterns of the two genes, as well as changes in expression levels under different atmospheric conditions (stress) were analyzed by real-time RT-PCR. Recombinant enzymes, expressed in E. coli were used for in vitro m...
Source: Molecular Biology Reports - October 8, 2009 Category: Molecular Biology Authors: Fasseas MK, Tsikou D, Flemetakis E, Katinakis P Tags: Mol Biol Rep Source Type: journals
Molecular cloning of the HGD gene and association of SNPs with meat quality traits in Chinese red cattle.Email this article to a colleague.
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Homogentisate 1, 2 dioxygenase (HGD) is one of six enzymes required for the catabolism of the aromatic amino acids phenylalanine and tyrosine. Here we present the nucleotide sequence of transcripts of the bovine HGD gene. The full length cDNA of bovine HGD has been identified, encoding a deduced protein of 445 amino acids (Accession No. FJ515744). The bovine HGD gene comprises 14 exons and 13 introns. This is the first published cDNA bovine sequences that share high sequence similarity with other species. Semi-quantitative RT-PCR analysis demonstrated that the bovine HGD transcript was mainly expressed in liver and kid...
Source: Molecular Biology Reports - October 8, 2009 Category: Molecular Biology Authors: Zhou G, Dudgeon C, Li M, Cao Y, Zhang L, Jin H Tags: Mol Biol Rep Source Type: journals
Effects of oral Lactobacillus plantarum on hepatocyte tight junction structure and function in rats with obstructive jaundice.Email this article to a colleague.
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Surgery and infection are prominent risk factors for the development of obstructive cholestasis which in turn is associated with failure of the liver barrier. We studied the effects of oral Lactobacillus plantarum (LP) supplementation on endotoxemia, oxidative stress, apoptosis, and tight junctions of hepatocytes in an experimental model of obstructive jaundice. Fifty male Wistar rats were randomly divided into five groups of 10 each: group I, sham-operated; group II, ligation and division of the common bile duct (BDL); group III, BLD followed by oral LP treatment; group IV, BDL followed by internal biliary drainage (I...
Source: Molecular Biology Reports - October 8, 2009 Category: Molecular Biology Authors: Zhang M, Wang XQ, Zhou YK, Ma YL, Shen TY, Chen HQ, Chu ZX, Qin HL Tags: Mol Biol Rep Source Type: journals
Automated quality assessment of autonomously acquired microscopic images of fluorescently stained bacteriaEmail this article to a colleague.
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We present an approach based on an artificial neural network (ANN) to assess the quality of such images. Spatially invariant estimators were extracted as ANN input data from subdivided images by low level image processing. Different ANN designs were compared and >400 ANNs were trained and tested on a set of 25,000 manually classified images. The optimal ANN featured a correct identification rate of 94% (3% false positives, 3% false negatives) and could process about 10 images per second. We compared its performance with the image quality assessment by different humans and discuss the difficulties in assigning images to the...
Source: Cytometry Part A - October 8, 2009 Category: Molecular Biology Authors: M. Zeder, E. Kohler, J. Pernthaler Source Type: journals
A routinely applicable way for using FCM in cell enumeration with CFSE-labeled CellBeads as internal standardEmail this article to a colleague.
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No abstract.
Source: Cytometry Part A - October 8, 2009 Category: Molecular Biology Authors: Fan-Fan Cao, Li-Min Xu, Bin Peng, Qiu-Hua Xie, Georges Uzan, Deng-Hai Zhang Source Type: journals
High content image cytometry in the context of subnuclear organizationEmail this article to a colleague.
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The organization of proteins in space and time is essential to their function. To accurately quantify subcellular protein characteristics in a population of cells with regard for the stochasticity of events in a natural context, there is a fast-growing need for image-based cytometry. Simultaneously, the massive amount of data that is generated by image-cytometric analyses, calls for tools that enable pattern recognition and automated classification. In this article, we present a general approach for multivariate phenotypic profiling of individual cell nuclei and quantification of subnuclear spots using automated fluorescen...
Source: Cytometry Part A - October 8, 2009 Category: Molecular Biology Authors: W. H. De Vos, L. Van Neste, B. Dieriks, G. H. Joss, P. Van Oostveldt Source Type: journals
Apolipoprotein A-I inhibits chemotaxis, adhesion, activation of THP-1 cells and improves the plasma HDL inflammatory index.Email this article to a colleague.
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The anti-inflammatory effects of high density lipoprotein (HDL) are well described, however, such effects of Apolipoprotein A-I (ApoA-I) are less studied. Building on our previous study, we further explored the mechanism of anti-inflammatory effects of ApoA-I, and focused especially on the interaction between monocyte and endothelial cells and plasma HDL inflammatory index in LPS-challenged rabbits. Our results show that ApoA-I significantly decreased LPS-induced MCP-1 release from THP-1 cells and ox-LDL-induced THP-1 migration ratio (P<0.01, respectively). ApoA-I significantly decreased sL-selectin, sICAM-1 and sVC...
Source: Cytokine - October 8, 2009 Category: Molecular Biology Authors: Wang L, Chen WZ, Wu MP Tags: Cytokine Source Type: journals
Cytokine expression in placenta-derived mesenchymal stem cells in patients with pre-eclampsia and normal pregnancies.Email this article to a colleague.
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Conclusions: The decreased levels of sICAM and SDF-1 found in the decidua-derived MSCs from pre-eclamptic pregnancies might be associated with some of the immunological alterations in pre-eclampsia.
PMID: 19819721 [PubMed - as supplied by publisher]
Source: Cytokine - October 8, 2009 Category: Molecular Biology Authors: Hwang JH, Lee MJ, Seok OS, Paek YC, Cho GJ, Seol HJ, Lee JK, Oh MJ Tags: Cytokine Source Type: journals
