Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 17.
'On-the-fly' optical encoding of combinatorial peptide libraries for profiling of protease specificity
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Lionel Marcon, Bronwyn J. Battersby, Andreas Ruhmann, Kym Ford, Matthew Daley, Gwendolyn A. Lawrie, Matt Trau
(Paper from Mol. BioSyst.)
Lionel Marcon, Mol. BioSyst., 2010, DOI: 10.1039/b909087h
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The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - October 5, 2009 Category: Molecular Biology Authors: Lionel MarconBronwyn J. Battersby Andreas Ruhmann Kym Ford Matthew Daley Gwendolyn A. Lawrie Matt Trau Source Type: journals
Synthesis of a photo-caged aminooxy alkane thiolEmail this article to a colleague.
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Rock J. Mancini, Ronald C. Li, Zachary P. Tolstyka, Heather D. Maynard
(Paper from Org. Biomol. Chem.)
Rock J. Mancini, Org. Biomol. Chem., 2009, DOI: 10.1039/b904195h
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Source: RSC - Organic Biomolecular Chemistry - October 5, 2009 Category: Molecular Biology Authors: Rock J. ManciniRonald C. Li Zachary P. Tolstyka Heather D. Maynard Source Type: journals
4-Aminoproline-based arginine-glycine-aspartate integrin binders with exposed ligation points: practical in-solution synthesis, conjugation and binding affinity evaluationEmail this article to a colleague.
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Lucia Battistini, Paola Burreddu, Paola Carta, Gloria Rassu, Luciana Auzzas, Claudio Curti, Franca Zanardi, Leonardo Manzoni, Elena M. V. Araldi, Carlo Scolastico, Giovanni Casiraghi
(Paper from Org. Biomol. Chem.)
Lucia Battistini, Org. Biomol. Chem., 2009, DOI: 10.1039/b914836a
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Source: RSC - Organic Biomolecular Chemistry - October 5, 2009 Category: Molecular Biology Authors: Lucia BattistiniPaola Burreddu Paola Carta Gloria Rassu Luciana Auzzas Claudio Curti Franca Zanardi Leonardo Manzoni Elena M. V. Araldi Carlo Scolastico Giovanni Casiraghi Source Type: journals
Expedient synthesis of 3-hydroxyisoquinolines and 2-hydroxy-1,4-naphthoquinones via one-pot aryne acyl-alkylation/condensationEmail this article to a colleague.
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Kevin M. Allan, Boram D. Hong, Brian M. Stoltz
(Paper from Org. Biomol. Chem.)
Kevin M. Allan, Org. Biomol. Chem., 2009, DOI: 10.1039/b913336d
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Source: RSC - Organic Biomolecular Chemistry - October 5, 2009 Category: Molecular Biology Authors: Kevin M. AllanBoram D. Hong Brian M. Stoltz Source Type: journals
The genome sequence of the protostome Daphnia pulex encodes respective orthologues of a neurotrophin, a Trk and a p75NTR: Evolution of neurotrophin signaling components and related proteins in the bilateria.Email this article to a colleague.
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Conclusions:
It appears that a neurotrophin, Trk and p75NTR existed at the protostome/deuterostome split. In protostomes, a "neurotrophin superfamily" includes Spzs and neurotrophins which respectively form two paralogous families. Trks and Trkl proteins also form closely related paralogous families within the protostomian RTKs, whereby Trkls are absent in deuterostomes. The finding of p75NTR in several protostomes suggests that death domain TNFR superfamily proteins appeared early in evolution.
Source: BMC Evolutionary Biology - Latest articles - October 5, 2009 Category: Molecular Biology Authors: Karen Wilson Source Type: journals
Finding Chimeras: A bioinformatic strategy for identification of cross-linked peptides.Email this article to a colleague.
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Chemical cross-linking, followed by identification of the cross-linked residues, is a powerful approach to probe the topologies and interacting surfaces of protein assemblies. In this work, we demonstrate a new bioinformatic approach using multiple program modules within the software package 'Protein Prospector' that greatly facilitates the discovery of cross-linked peptides in chemical cross-linking studies. Examples are given for how this approach has been used for defining interfaces in heterodimeric and homodimeric protein complexes, both of which provide results in close agreement with crystal structures, verifyin...
Source: Molecular and Cellular Proteomics : MCP - October 5, 2009 Category: Molecular Biology Authors: Chu F, Baker PR, Burlingame AL, Chalkley RJ Tags: Mol Cell Proteomics Source Type: journals
Nucleotide sequence and expression of the 14-3-3 from the halotolerant alga Dunaliella salina.Email this article to a colleague.
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Previously we reported the nucleotide sequence of a 14-3-3 cDNA cloned from the unicellular green alga Dunaliella salina, however, the nucleotide sequence of this gene have not been reported so far. In the present study, the cloning and characterization of the nucleotide sequence, the gene copy and expression were undertaken. The coding sequence of the gene was found to be interrupted by five introns of 132, 266, 153, 152 and 625 bp, respectively. Introns 3-5 were found in conserved positions as compared to the Chlamydomonas reinhardtii 14-3-3 gene. D. salina 14-3-3 cDNA was inserted into the prokaryotic expression pla...
Source: Molecular Biology Reports - October 5, 2009 Category: Molecular Biology Authors: Wang TY, Jing CQ, Dong WH, Zhang JH, Zhang Y Tags: Mol Biol Rep Source Type: journals
Involvement of NF-kappa B pathway in TL1A gene expression induced by lipopolysaccharide.Email this article to a colleague.
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Conclusion: (1) LPS induces TL1A expression through the transcriptional activation via a NF-kappa B pathway. (2) The NF-kappa B binding site in the 5' flanking region of TL1A was identified.
PMID: 19815424 [PubMed - as supplied by publisher]
Source: Cytokine - October 5, 2009 Category: Molecular Biology Authors: Endo K, Kinouchi Y, Kakuta Y, Ueki N, Takahashi S, Shimosegawa T Tags: Cytokine Source Type: journals
Supervised learning with decision tree-based methods in computational and systems biologyEmail this article to a colleague.
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Pierre Geurts, Alexandre Irrthum, Louis Wehenkel
(Review from Mol. BioSyst.)
Pierre Geurts, Mol. BioSyst., 2009, DOI: 10.1039/b907946g
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Source: RSC - Mol. BioSyst. latest articles - October 4, 2009 Category: Molecular Biology Authors: Pierre GeurtsAlexandre Irrthum Louis Wehenkel Source Type: journals
Stereodivergent syntheses at the glucose backboneEmail this article to a colleague.
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Jian Yin, Torsten Linker
(Communication from Org. Biomol. Chem.)
Jian Yin, Org. Biomol. Chem., 2009, DOI: 10.1039/b918893m
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Source: RSC - Organic Biomolecular Chemistry - October 4, 2009 Category: Molecular Biology Authors: Jian YinTorsten Linker Source Type: journals
Synthesis and X-ray structural analysis of platinum and ethynyl-platinum corannulenes: supramolecular tectonsEmail this article to a colleague.
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Roman Maag, Brian H. Northrop, Anna Butterfield, Anthony Linden, Oliver Zerbe, Young Min Lee, Ki-Whan Chi, Peter J. Stang, Jay S. Siegel
(Paper from Org. Biomol. Chem.)
Roman Maag, Org. Biomol. Chem., 2009, DOI: 10.1039/b916020e
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Source: RSC - Organic Biomolecular Chemistry - October 4, 2009 Category: Molecular Biology Authors: Roman MaagBrian H. Northrop Anna Butterfield Anthony Linden Oliver Zerbe Young Min Lee Ki-Whan Chi Peter J. Stang Jay S. Siegel Source Type: journals
The pyridinone-methide eliminationEmail this article to a colleague.
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Rotem Perry-Feigenbaum, Phil S. Baran, Doron Shabat
(Communication from Org. Biomol. Chem.)
Rotem Perry-Feigenbaum, Org. Biomol. Chem., 2009, DOI: 10.1039/b915265b
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Source: RSC - Organic Biomolecular Chemistry - October 4, 2009 Category: Molecular Biology Authors: Rotem Perry-FeigenbaumPhil S. Baran Doron Shabat Source Type: journals
l-Proline-catalyzed synthesis of highly functionalized multisubstituted 1,4-dihydropyridinesEmail this article to a colleague.
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Huanfeng Jiang, Ronghuan Mai, Hua Cao, Qiuhua Zhu, Xiaohang Liu
(Paper from Org. Biomol. Chem.)
Huanfeng Jiang, Org. Biomol. Chem., 2009, DOI: 10.1039/b914659h
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Source: RSC - Organic Biomolecular Chemistry - October 4, 2009 Category: Molecular Biology Authors: Huanfeng JiangRonghuan Mai Hua Cao Qiuhua Zhu Xiaohang Liu Source Type: journals
Amphioxus (Branchiostoma floridae) has orthologs of vertebrate odorant receptorsEmail this article to a colleague.
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Conclusions:
Here, we show that amphioxus has orthologs of vertebrate ORs. This conclusion demonstrates that the receptors, and perhaps other components of vertebrate olfaction, evolved at least 550 million years ago. We have also identified highly conserved amino acid motifs that may be important for maintaining receptor conformation or regulating receptor activity. We anticipate that the identification of vertebrate OR orthologs in amphioxus will lead to an improved understanding of OR gene family evolution, OR gene function, and the mechanisms that control cell-specific expression, axonal guidance, signal transduction a...
Source: BMC Evolutionary Biology - Latest articles - October 4, 2009 Category: Molecular Biology Authors: Allison ChurcherJohn Taylor Source Type: journals
Computational modelling of cancerous mutations in the EGFR/ERK signalling pathwayEmail this article to a colleague.
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Conclusions:
Our results suggest that different routes to ERK activation are being utilised in different cancerous situations which therefore has interesting implications for drug selection strategies. We also conducted a comparison of the critical differences between signalling from different growth factor receptors (namely EGFR, mutated EGFR, NGF, and Insulin) with our results suggesting the difference between the systems are large scale and can be attributed to the presence/absence of entire pathways rather than subtle difference in individual rate constants between the systems.
Source: BMC Systems Biology - Latest articles - October 4, 2009 Category: Molecular Biology Authors: Richard OrtonMichiel AdriaensAmelie GormandOliver SturmWalter KolchDavid Gilbert Source Type: journals
CAZIP, a novel protein expressed in the developing heart and nervous systemEmail this article to a colleague.
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Recently, we have performed a whole genome micro-array analysis on human embryonic stem cells differentiating toward cardiomyocytes, which resulted in the identification of novel genes that were highly up-regulated during differentiation. Here, we describe one of these novel genes annotated as KIAA0774. The predicted protein contains a leucine-zipper domain at the C-terminus and has at least two isoforms (358 and 1354 amino acids). Whole-mount in situ hybridization confirmed that the mRNA of both the mouse and chicken orthologs of KIAA0774 is expressed during early cardiac development. Hence, we named this protein CAZIP (c...
Source: Developmental Dynamics - October 4, 2009 Category: Molecular Biology Authors: Leonie Du Puy, Abdelaziz Beqqali, Jantine Monshouwer-Kloots, Henk P. Haagsman, Bernard A.J. Roelen, Robert Passier Source Type: journals
Patterns of Wnt pathway activity in the mouse incisor indicate absence of Wnt/[beta]-catenin signaling in the epithelial stem cellsEmail this article to a colleague.
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The Wnt pathway is crucial for tooth development as shown by dental defects caused by impaired Wnt signaling in mouse and human. We investigated Wnt signaling in continuously growing mouse incisors focusing on epithelial stem cells. Ten Wnt ligands were expressed both in the dental epithelium and mesenchyme, and were associated mainly with odontoblast and ameloblast differentiation. Wnt/[beta]-catenin activity was detected in mesenchyme in BATgal and TOPgal reporter mice while Axin2, also a reporter of Wnt/[beta]-catenin signaling, was expressed additionally in the epithelium. Axin2 was, however, excluded from the epitheli...
Source: Developmental Dynamics - October 4, 2009 Category: Molecular Biology Authors: Marika Suomalainen, Irma Thesleff Source Type: journals
Quantitative nano-proteomics for protein complexes (QNanoPX) related to estrogen transcriptional action.Email this article to a colleague.
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We developed an integrated proteomics approach using a chemically functionalized gold nanoparticle (AuNP) as a novel probe for affinity purification in order to analyze a large protein complex in vivo. We then applied this approach to globally map the transcriptional activation complex of the estrogen response element (ERE). This approach was designated as Quantitative Nano-Proteomics for Protein complexes (QNanoPX). In this approach, the positive AuNP-ERE probes were functionalized with polyethylene glycol (PEG) and the consensus sequence of ERE and negative AuNP-PEG probes were functionalized with PEG without the ERE...
Source: Molecular and Cellular Proteomics : MCP - October 4, 2009 Category: Molecular Biology Authors: Cheng PC, Chang HK, Chen SH Tags: Mol Cell Proteomics Source Type: journals
Regulation of protein kinase A (PKA) activity by p90 ribosomal S6 kinase 1 (RSK1).Email this article to a colleague.
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Previously, we reported that the catalytic subunit of cyclic AMP-dependent protein kinase (PKAc) binds to the active p90 ribosomal S6 kinase 1 (RSK1) (Chaturvedi et al., Mol. Cell. Biol. 2006, Vol. 26, 4586-4600). Herein, by overexpressing HA-tagged RSK1 fragments in HeLa cells we have identified the region of RSK1 that is responsible for the interaction with PKAc. PKAc bound to the last 13 amino acids of RSK1, which overlaps ERK1/2 docking site. This interaction between PKAc and RSK1 required the phosphorylation of Ser732 in the C-terminus of RSK1. Depending upon its phosphorylation status, RSK1 switched interactions ...
Source: Mol Biol Cell - October 4, 2009 Category: Molecular Biology Authors: Gao X, Patel TB Tags: J Biol Chem Source Type: journals
Oxidative stress in bone remodelling and disease.Email this article to a colleague.
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Oxidative stress is characterised by an increased level of reactive oxygen species (ROS) that disrupts the intracellular reduction-oxidation (redox) balance. Although initially shown to be involved in aging, physiological roles for ROS in regulating cell functions and mediating intracellular signals have emerged. In bone tissues, recent studies have demonstrated that ROS generation is a key modulator of bone cell function and that oxidative status influences the pathophysiology of mineralised tissues. Here, we review the crucial role of oxidative stress in bone pathophysiology, and discuss the possibility that ROS prod...
Source: Trends in Molecular Medicine - October 4, 2009 Category: Molecular Biology Authors: Wauquier F, Leotoing L, Coxam V, Guicheux J, Wittrant Y Tags: Trends Mol Med Source Type: journals
Irritable bowel syndrome: towards biomarker identification.Email this article to a colleague.
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Irritable bowel syndrome (IBS), the most common functional gastrointestinal disorder referred to gastroenterologists, affects 7-10% of the general population worldwide. The lack of suitable disease-defining biological markers coupled with a poorly understood underlying pathophysiology complicates patient diagnosis and seriously hampers drug discovery efforts. Over the past few years, a number of potential biomarkers have emerged, and in this review we critically evaluate such candidates. In particular, we highlight the increasing number of studies supporting a low-grade immune activation in IBS and consider how the lat...
Source: Trends in Molecular Medicine - October 4, 2009 Category: Molecular Biology Authors: Clarke G, Quigley EM, Cryan JF, Dinan TG Tags: Trends Mol Med Source Type: journals
Phase II nonrandomized study of the efficacy and safety of COX-2 inhibitor celecoxib on patients with cancer cachexiaEmail this article to a colleague.
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Abstract Chronic inflammation is one of the main features of cancer cachexia. Experimental and clinical studies showed that cyclooxygenase-2
inhibitors, such as celecoxib, may be beneficial in counteracting major symptoms of this devastating syndrome. We carried
out a prospective phase II clinical trial to test the safety and effectiveness of an intervention with the COX-2 inhibitor
celecoxib (300 mg/day for 4 months) on key variables of cachexia (lean body mass, resting energy expenditure, serum levels
of proinflammatory cytokines, and fatigue) in patients with advanced cancer at different sites. ...
Source: Journal of Molecular Medicine - October 3, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Flow cytometry without alignment of collection opticsEmail this article to a colleague.
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This study describes the performance of a new waveguide flow cell constructed from Teflon AF® (TFC) and the potential use of fiber optic splitters to replace collection objectives and dichroic mirrors. The TFC has the unique optical property that the refractive index of the polymer is lower than water and therefore, water filled TFC behaves and functions as a liquid core waveguide. Thus, as cells flow through the TFC and are illuminated by a laser orthogonal to the flow direction, scattered and fluorescent light is directed down the axis of the TFC to a fiber optic. The total signal in the fiber optic is then split into m...
Source: Cytometry Part A - October 2, 2009 Category: Molecular Biology Authors: Greg Sitton, Friedrich Srienc Source Type: journals
Stem cells for stress urinary incontinence: the adipose promise.Email this article to a colleague.
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Abstract Stress urinary incontinence (SUI), the most common type of incontinence in women, is a frequent and costly ailment responsible for an alteration to the quality of life. While medical treatment give some rather deceiving results, surgical techniques that include colposuspension or tension-free vaginal tape, employed in cases of urethral support defect, give a 5 year cure rate of more than 80%. However, these techniques could lead to complications or recurrence of symptoms. Recently, the initiation of urethral cell therapy has been undertaken by doctors and researchers. One principal source of autologous adult s...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Roche R, Festy F, Fritel X Tags: J Cell Mol Med Source Type: journals
Neurotrophic factors promote cholinergic differentiation in human embryonic stem cell-derived neurons.Email this article to a colleague.
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ABSTRACT Cholinergic neurotransmission is essential for many important functions in the brain, including cognitive mechanisms. Here we demonstrate that human embryonic stem (hES) cells differentiate into a population of neuronal cells that express the cholinergic enzyme choline acetyltransferase (ChAT) and homeobox proteins specifying neuronal progenitors of ventral telencephalic lineage. These differentiated cells express transcripts for cholinergic alpha3, alpha4 and alpha7 nicotinic acetylcholine receptor (nAChR) subunits and for M1, M2 and M3 muscarinic acetylcholine receptor (mAChR) subtypes. Stimulation with brai...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Nilbratt M, Porras O, Marutle A, Hovatta O, Nordberg A Tags: J Cell Mol Med Source Type: journals
Aberrant human leukocyte antigen-G expression and its clinical relevance in hepatocellular carcinoma.Email this article to a colleague.
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Abstract The clinical relevance of human leukocyte antigen-G (HLA-G) has been postulated in malignancies. Hepatocellular carcinoma (HCC) is a major contributor to cancer incidence and mortality worldwide; however, potential roles of HLA-G in HCC remain unknown. In the current study, HLA-G expression in 219 primary HCC lesions and their adjacent non-tumorous samples was analyzed with immuno- histochemistry. Correlations between HLA-G expression and various clinical parameters were evaluated. Meanwhile, functional analysis of transfected cell surface HLA-G expression on NK cell cytolysis was performed in vitro. HLA-G exp...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Lin A, Chen HX, Zhu CC, Zhang X, Xu HH, Zhang JG, Wang Q, Zhou WJ, Yan WH Tags: J Cell Mol Med Source Type: journals
The actin filament cross-linker L-plastin confers resistance to TNF-alpha in MCF-7 breast cancer cells in a phosphorylation-dependent manner.Email this article to a colleague.
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Abstract We used a TNF-resistant breast adenocarcinoma MCF-7 cell line to investigate the involvement of the actin cytoskeleton in the mechanism of cell resistance to this cytokine. We found that TNF-resistance correlates with the loss of cell epithelial properties and the gain of a mesenchymal phenotype, reminiscent of an epithelial-to-mesenchymal transition (EMT). Morphological changes were associated with a profound reorganization of the actin cytoskeleton and with a change in the repertoire of expressed actin cytoskeleton genes and EMT markers, as revealed by DNA microarray-based expression profiling. L-plastin, an...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Janji B, Vallar L, Al-Tanoury Z, Bernardin F, Vetter G, Schaffner-Reckinger E, Berchem G, Friederich E, Chouaib S Tags: J Cell Mol Med Source Type: journals
Translational up-regulation of Aurora-A in EGFR-overexpressed cancer.Email this article to a colleague.
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In this study, we demonstrated that EGF signaling increased the Aurora-A protein expression in EGFR-overexpressed colorectal cancer cell lines via increasing the translational efficiency. In addition, the overexpression of EGFR was also associated with higher expression of Aurora-A in clinical colorectal samples. Activation of the PI3K/Akt/mTOR and MEK/ERK pathways mediated the effect of EGF induced translational up-regulation. Besides, only the splicing variants containing exon 2 of Aurora-A mRNA showed increased interaction with the translational complex to synthesize Aurora-A protein under EGF stimulus. Besides, the exo...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Lai CH, Tseng JT, Lee YC, Chen YJ, Lee JC, Huang TC, Liu YW, Leu TH, Liu YW, Chen YP, Chang WC, Hung LY Tags: J Cell Mol Med Source Type: journals
Mesenchymal stem cells modified to express lentivirus-TNFalpha-Tumstatin inhibit the growth of prostate cancer.Email this article to a colleague.
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Abstract Mesenchymal stem cells (MSCs) are a potential novel delivery system for cell-based gene therapies. Although TNFalpha has been shown to have antitumor activity, its use for therapy is limited by its systemic toxicity. For the present study, we designed lentivirus-mediated signal peptide TNFalpha-Tumstatin(45-132)-expressing mesenchymal stem cells (SPTT-MSCs) as a novel anticancer approach. We evaluated the effects of this approach on human prostate cancer cells (PC3 and LNCaP) by co-culturing them with either SPTT-MSCs or supernatants from their culture medium in vitro. The antitumor effects and possible mechan...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Zhang X, Xu W, Qian H, Zhu W, Zhang R Tags: J Cell Mol Med Source Type: journals
Co-ordinated autophagy with resveratrol and gamma-tocotrienol confers synergetic cardioprotection.Email this article to a colleague.
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This study compared two dietary phytochemicals, grape-derived resveratrol and palm oil-derived gamma-tocotrienol, either alone or in combination, on the contribution of autophagy in cardioprotection during ischemia and reperfusion. Sprague Dawley rats weighing between 250-300 gm were randomly assigned to one of the following groups: vehicle, ischemia/reperfusion (I/R), resveratrol+I/R, gamma-tocotrienol+I/R, resveratrol+gamma-tocotrienol+I/R. For resveratrol treatments, the rats were gavaged with resveratrol [2.5 mg/kg] for 15 days while for gamma-tocotrienol experiments the rats were gavaged with gamma-tocotrienol [0.3 mg...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Lekli I, Ray D, Mukherjee S, Gurusamy N, Ahsan MK, Juhasz B, Bak I, Tosaki A, Gherghiceanu M, Popescu LM, Das DK Tags: J Cell Mol Med Source Type: journals
Functional characterization of the progestagen-associated endometrial protein gene in human melanoma.Email this article to a colleague.
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Abstract Utilizing gene microarray profiling of melanoma samples, we have recently identified a novel gene over-expressed in both thick primary and metastatic melanomas. This gene, progestagen-associated endometrial protein (PAEP), has never before been implicated in the oncogenic processes of melanoma, with its true function in oncogenesis and tumor progression relatively unknown. Over-expression of the PAEP gene in freshly procured thick primary and metastatic melanoma samples (58%) and daughter cell lines (77%) is confirmed by quantitative RT-PCR, immunohistochemisty, Western blotting and mass spectrometric analysis...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Ren S, Liu S, Howell PM, Zhang G, Pannell L, Samant R, Shevde-Samant L, Tucker JA, Fodstad O, Riker AI Tags: J Cell Mol Med Source Type: journals
Hematopoietic stem cells in spleen have distinct differentiative potential for antigen presenting cells.Email this article to a colleague.
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In this study, neonatal spleen showed multilineage hematopoietic reconstitution in mouse chimeras, rather than specific or restricted ability to differentiate into L-DC. However, the representation of individual APC subsets was found to be unequal in chimeras partially reconstituted with donor cells, such that more donor-derived progeny were seen for L-DC than for myeloid and cDC subsets. The ability of HSC in spleen to develop into L-DC was indicated by a strong bias in the subset size of these cells over other splenic APC subsets. This type of evidence supports a model whereby spleen represents an important site for hema...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Tan JK, O'Neill HC Tags: J Cell Mol Med Source Type: journals
Familial and Racial Determinants of Tumor Suppressor Genes Promoter Hypermethylation in Breast Tissues from Healthy Women.Email this article to a colleague.
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Conclusions: Gene promoter hypermethylation was commonly found in healthy breast tissues from women without cancer, indicating that these events are frequent and early lesions. Race and family history of cancer increase the likelihood of these early events.
PMID: 19799643 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Dumitrescu RG, Marian C, Krishnan SS, Spear SL, Kallakury BV, Perry DJ, Convit JR, Seillier-Moiseiwitsch F, Yang Y, Freudenheim JL, Shields PG Tags: J Cell Mol Med Source Type: journals
Leberagin-C, a disintegrin-like/cysteine rich protein from Macrovipera lebetina transmediterranea venom, inhibits alpha-v-beta-3 integrin-mediated cell adhesion.Email this article to a colleague.
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Leberagin-C, a new member of the disintegrin-like/cysteine-rich (D/C) family, was purified to homogeneity from the venom of Tunisian snake Macrovipera lebetina transmediterranea. It is a monomeric protein with a molecular mass of 25,787Da. Its complete sequence of 205 amino acids residues was established by cDNA cloning. The leberagin-C shows many conserved sequences with other known D/C proteins, like the SECD binding sites and a pattern of 28 cysteines. It is the first purified protein from Macrovipera lebetina transmediterranea with only two disintegrin-like/cysteine-rich domains. Leberagin-C is able to inhibit plat...
Source: Matrix Biology - October 2, 2009 Category: Molecular Biology Authors: Limam I, Bazaa A, Srairi-Abid N, Taboubi S, Jebali J, Zouari-Kessentini R, Kallech-Ziri O, Mejdoub H, Hammami A, El Ayeb M, Luis J, Marrakchi N Tags: Matrix Biol Source Type: journals
A Novel Fluorescent Imaging Agent for Diffuse Optical Tomography of the Breast: First Clinical Experience in PatientsEmail this article to a colleague.
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Conclusions Lowest doses of Omocianine performed best in lesion detection; DOT using a low-dose fluorescent agent is feasible and safe
for breast cancer visualization in patients.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0269-1Authors
Stephanie van de Ven, University Medical Center Utrecht Department of Radiology Utrecht The NetherlandsAndrea Wiethoff, Philips Healthcare Best The NetherlandsTim Nielsen, Philips Research Europe Eindhoven The NetherlandsBernhard Brendel, Philips Research Europe Eindhoven The NetherlandsMarjolein van der Voort, Philips Research Europe Ph...
Source: Molecular Imaging and Biology - October 2, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
68Ga-Chloride PET Reveals Human Pancreatic Adenocarcinoma Xenografts in Rats—Comparison with FDGEmail this article to a colleague.
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Conclusions
68Ga-chloride delineated subcutaneously implanted pancreatic adenocarcinoma xenografts by PET, but the uptake was lower than
FDG. Further studies to clarify the value of 68Ga-chloride for PET imaging of tumors are warranted.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0267-3Authors
Tiina Ujula, Turku University Hospital Turku PET Centre 20521 Turku FinlandSatu Salomäki, Turku University Hospital Turku PET Centre 20521 Turku FinlandAnu Autio, Turku University Hospital Turku PET Centre 20521 Turku FinlandPauliina Luoto, Turku University Hospital Turku PET Ce...
Source: Molecular Imaging and Biology - October 2, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
E2F2 suppresses Myc-induced proliferation and tumorigenesisEmail this article to a colleague.
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Deregulation of E2F transcriptional activity as a result of alterations in the p16-cyclin D-Rb pathway is a hallmark of cancer. However, the roles of the different E2F family members in the process of tumorigenesis are still being elucidated. Studies in mice and humans suggest that E2F2 functions as a tumor suppressor. Here we demonstrate that E2f2 inactivation cooperates with transgenic expression of Myc to enhance tumor development in the skin and oral cavity. In fact, hemizygosity at the E2f2 locus was sufficient to increase tumor incidence in this model. Loss of E2F2 enhanced proliferation in Myc transgenic tissue but ...
Source: Molecular Carcinogenesis - October 1, 2009 Category: Molecular Biology Authors: Raju V. Pusapati, Regina L. Weaks, Robert J. Rounbehler, Mark J. McArthur, David G. Johnson Source Type: journals
The powerful law of the power law and other myths in network biologyEmail this article to a colleague.
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Gipsi Lima-Mendez, Jacques van Helden
(Review from Mol. BioSyst.)
Gipsi Lima-Mendez, Mol. BioSyst., 2009, DOI: 10.1039/b908681a
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Source: RSC - Mol. BioSyst. latest articles - October 1, 2009 Category: Molecular Biology Authors: Gipsi Lima-MendezJacques van Helden Source Type: journals
Microwave-assisted versatile hydrogenation of carbonyl compounds using supported metal nanoparticlesEmail this article to a colleague.
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Maria Jose Gracia, Juan Manuel Campelo, Elia Losada, Rafael Luque, Jose Maria Marinas, Antonio Angel Romero
(Communication from Org. Biomol. Chem.)
Maria Jose Gracia, Org. Biomol. Chem., 2009, DOI: 10.1039/b913695a
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Source: RSC - Organic Biomolecular Chemistry - October 1, 2009 Category: Molecular Biology Authors: Maria Jose GraciaJuan Manuel Campelo Elia Losada Rafael Luque Jose Maria Marinas Antonio Angel Romero Source Type: journals
Profiling of polychromatic flow cytometry data on B-cells reveals patients' clusters in common variable immunodeficiencyEmail this article to a colleague.
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The aim of this study was to find an objective computational approach for phenotype analysis of common variable immunodeficiency (CVID) patients that describes all differences in the six-color space and to form groups of patients using computational methods. CVID is a heterogeneous primary immunodeficiency disorder where molecular defect is recognized in
Source: Cytometry Part A - October 1, 2009 Category: Molecular Biology Authors: Tomá[scaron] Kalina, Jan Stuchlý, Ale[scaron] Janda, Ond[rcaron]ej Hru[scaron]ák, [Scaron]árka R[uring][zcaron]i[ccaron]ková, Anna [Scaron]edivá, Ji[rcaron]í Litzman, Marcela Vlková Source Type: journals
ABCG2-associated resistance to Hoechst 33342 and topotecan in a murine cell model with constitutive expression of side population characteristicsEmail this article to a colleague.
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Drug resistant tumor "side-populations," enriched in cancer stem cells and identified by reduced accumulation of Hoechst 33342 under ABCG2-mediated efflux, may compromise therapeutic outcome. Side-population cells have predicted resistance to minor groove ligands, including the DNA topoisomerase I poison topotecan. We have used a stable Hoechst 33342-resistant murine L cell system (HoeR415) to study resistance patterns, removing the need for SP isolation before microarray analysis of gene expression and the tracking of cell cycle dynamics and cytotoxicity. The majority of HoeR415 cells displayed a side-population phenotype...
Source: Cytometry Part A - October 1, 2009 Category: Molecular Biology Authors: Paul J. Smith, Emeline Furon, Marie Wiltshire, Lee Campbell, Graham P. Feeney, Ronald D. Snyder, Rachel J. Errington Source Type: journals
Multipurpose modular lentiviral vectors for RNA interference and transgene expression.Email this article to a colleague.
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We have created a multipurpose modular lentiviral vector system for expressing both transgenes and miRNA 30-based short hairpins (shRNAmirs) for RNAi. The core of the resulting vector system, pLVmir, allows a simple two step cloning procedure for expressing shRNAmirs under the control of a Pol II promoter in both a constitutive and conditional manner. The adapted cloning method includes a PCR-free method for transferring shRNAmir based RNAi clones from a publicly available library (Open Biosystems). The addition of a Pol II promoter-driven shRNAmir cassette and broadening the choice of Pol III promoters and silencing t...
Source: Molecular Biology Reports - October 1, 2009 Category: Molecular Biology Authors: Kesireddy V, van der Ven PF, Fürst DO Tags: Mol Biol Rep Source Type: journals
Thrombospondins function as regulators of angiogenesis.Email this article to a colleague.
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Thrombospondins (TSPs) -1 and -2 were among the first protein inhibitors of angiogenesis to be identified, a property that was subsequently attributed to the interactions of sequences in their type I repeats with endothelial cell-surface receptors. The interactions of TSPs-1 and -2 with cell-surface receptors, proteases, growth factors, and other bioactive molecules, coupled with the absence of direct structural functions that can be attributed to these matrix proteins, qualify them for inclusion in the category of 'matricellular proteins'. The phenotypes of TSP-1, TSP-2, and double TSP-1/2-null mice confirm the roles ...
Source: Journal of Cell Communication and Signaling - October 1, 2009 Category: Molecular Biology Authors: Bornstein P Tags: J Cell Commun Signal Source Type: journals
The role of SPARC in extracellular matrix assembly.Email this article to a colleague.
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SPARC is a collagen-binding matricellular protein. Expression of SPARC in adult tissues is frequently associated with excessive deposition of collagen and SPARC-null mice fail to generate a robust fibrotic response to a variety of stimuli. This review summarizes recent advancements in the characterization of the binding of SPARC to collagens and describes the results of studies that implicate a function for SPARC in the regulation of the assembly of basal lamina and fibrillar collagen in the ECM. Potential cellular mechanisms that underlie SPARC activity in ECM deposition are also explored.
PMID: 19798598 [PubMed -...
Source: Journal of Cell Communication and Signaling - October 1, 2009 Category: Molecular Biology Authors: Bradshaw AD Tags: J Cell Commun Signal Source Type: journals
The many facets of the matricelluar protein periostin during cardiac development, remodeling, and pathophysiology.Email this article to a colleague.
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Periostin is a member of a growing family of matricellular proteins, defined by their ability to interact with components of the extracellular milieu, and with receptors at the cell surface. Through these interactions, periostin has been shown to play a crucial role as a profibrogenic molecule during tissue morphogenesis. Tissues destined to become fibrous structures are dependent on cooperative interactions between periostin and its binding partners, whereas in its absence, these structures either totally or partially fail to become mature fibrous entities. Within the heart, fibrogenic differentiation is required for ...
Source: Journal of Cell Communication and Signaling - October 1, 2009 Category: Molecular Biology Authors: Norris RA, Moreno-Rodriguez R, Hoffman S, Markwald RR Tags: J Cell Commun Signal Source Type: journals
SPARC functions as an inhibitor of adipogenesis.Email this article to a colleague.
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Adipogenesis, a key step in the pathogenesis of obesity, involves extensive ECM remodeling, changes in cell-ECM interactions, and cytoskeletal rearrangement. Matricellular proteins regulate cell-cell and cell-ECM interactions. Evidence in vivo and in vitro indicates that the prototypic matricellular protein, SPARC, inhibits adipogenesis and promotes osteoblastogenesis. Herein we discuss mechanisms underlying the inhibitory effect of SPARC on adipogenesis. SPARC enhances the Wnt/beta-catenin signaling pathway and regulates the expression and posttranslational modification of collagen. SPARC might drive preadipocytes awa...
Source: Journal of Cell Communication and Signaling - October 1, 2009 Category: Molecular Biology Authors: Nie J, Sage EH Tags: J Cell Commun Signal Source Type: journals
Fibulin-5, an integrin-binding matricellular protein: its function in development and disease.Email this article to a colleague.
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Interactions between the extracellular matrix (ECM) and cells are critical in embryonic development, tissue homeostasis, physiological remodeling, and tumorigenesis. Matricellular proteins, a group of ECM components, mediate cell-ECM interactions. One such molecule, Fibulin-5 is a 66-kDa glycoprotein secreted by various cell types, including vascular smooth muscle cells (SMCs), fibroblasts, and endothelial cells. Fibulin-5 contributes to the formation of elastic fibers by binding to structural components including tropoelastin and fibrillin-1, and to cross-linking enzymes, aiding elastic fiber assembly. Mice deficient ...
Source: Journal of Cell Communication and Signaling - October 1, 2009 Category: Molecular Biology Authors: Yanagisawa H, Schluterman MK, Brekken RA Tags: J Cell Commun Signal Source Type: journals
Thrombopoietic-mesenchymal interaction that may facilitate both endochondral ossification and platelet maturation via CCN2.Email this article to a colleague.
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In this study, we initially pursued the possible origin of the CCN2 in platelets. First, we examined if the CCN2 in platelets was produced by megakaryocyte progenitors during differentiation. Unexpectedly, neither megakaryocytic CMK cells nor megakaryocytes that had differentiated from human haemopoietic stem cells in culture showed any detectable CCN2 gene expression or protein production. Together with the fact that no appreciable CCN2 was detected in megakaryocytes in vivo, these results suggest that megakaryocytes themselves do not produce CCN2. Next, we suspected that mesenchymal cells situated around megakaryocytes i...
Source: Journal of Cell Communication and Signaling - October 1, 2009 Category: Molecular Biology Authors: Sumiyoshi K, Kubota S, Furuta RA, Yasui K, Aoyama E, Kawaki H, Kawata K, Ohgawara T, Yamashiro T, Takigawa M Tags: J Cell Commun Signal Source Type: journals
The role of osteopontin in inflammatory processes.Email this article to a colleague.
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Osteopontin (OPN) is a matricellular protein that mediates diverse biological functions. OPN is involved in normal physiological processes and is implicated in the pathogenesis of a variety of disease states, including atherosclerosis, glomerulonephritis, cancer, and several chronic inflammatory diseases. Through interactions with several integrins, OPN mediates cell migration, adhesion, and survival in many cell types. OPN also functions as a Th1 cytokine, promotes cell-mediated immune responses, and plays a role in chronic inflammatory and autoimmune diseases. Besides its function in inflammation, OPN is also a regul...
Source: Journal of Cell Communication and Signaling - October 1, 2009 Category: Molecular Biology Authors: Lund SA, Giachelli CM, Scatena M Tags: J Cell Commun Signal Source Type: journals
Thrombospondins in the heart: potential functions in cardiac remodeling.Email this article to a colleague.
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Cardiac remodeling after myocardial injury involves inflammation, angiogenesis, left ventricular hypertrophy and matrix remodeling. Thrombospondins (TSPs) belong to the group of matricellular proteins, which are non-structural extracellular matrix proteins that modulate cell-matrix interactions and cell function in injured tissues or tumors. They interact with different matrix and membrane-bound proteins due to their diverse functional domains. That the expression of TSPs strongly increases during cardiac stress or injury indicates an important role for them during cardiac remodeling. Recently, the protective propertie...
Source: Journal of Cell Communication and Signaling - October 1, 2009 Category: Molecular Biology Authors: Schellings MW, van Almen GC, Sage EH, Heymans S Tags: J Cell Commun Signal Source Type: journals
