Molecular Biology
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. 
Subscribe to this data using MyMedWorm.
Subscribe to this data using GoogleReader.
Subscribe to this data using Bloglines.
Subscribe to this data using MyYahoo.
Get the very latest Swine Flu news via the MedWorm Swine Flu RSS news feed - updated hourly from thousands of authoritative health and news sources.
This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 20.
Immobilization of the Glycosylphosphatidylinositol-anchored Gas1 Protein into the Chitin Ring and Septum Is Required for Proper Morphogenesis in Yeast.
Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
We present evidence that the Crh1p and Crh2p are required for tethering Gas1p to the chitin ring and bud scar. These results reveal a new mechanism of protein immobilization at specific sites of the cell envelope.
PMID: 19793924 [PubMed - as supplied by publisher]
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Rolli E, Ragni E, Calderon J, Porello S, Fascio U, Popolo L Tags: Mol Biol Cell Source Type: journals
The Cdc42 Effectors Ste20, Cla4 and Skm1 Down-regulate the Expression of Genes Involved in Sterol Uptake by a MAPK-independent Pathway.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: Charles Boone In S. cerevisiae, the Rho-type GTPase Cdc42 regulates polarized growth through its effectors, including the p21-activated kinases (PAKs) Ste20, Cla4 and Skm1. Previously, we demonstrated that Ste20 interacts with several proteins involved in sterol synthesis that are crucial for cell polarization. Under anaerobic conditions, sterols cannot be synthesized and need to be imported into cells. Here, we show that Ste20, Cla4 and Skm1 form a complex with Sut1, a transcriptional regulator that promotes sterol uptake. All three PAKs can translocate into the nucleus and down-regulate the express...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Lin M, Unden H, Jacquier N, Schneiter R, Just U, Höfken T Tags: Mol Biol Cell Source Type: journals
Pancreatic Ductal Morphogenesis and the Pdx-1 Homeodomain Transcription Factor.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: M. Bishr Omary Embryonic development of the pancreas is marked by an early phase of dramatic morphogenesis, in which pluripotent progenitor cells of the developing pancreatic epithelium give rise to the full array of mature exocrine and endocrine cell types. The genetic determinants of acinar and islet cell lineages are somewhat well defined, however the molecular mechanisms directing ductal formation and differentiation remain to be elucidated. The complex ductal architecture of the pancreas is established by a reiterative program of progenitor cell expansion and migration known as branching morphog...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Wescott MP, Rovira M, Reichert M, von Burstin J, Means A, Leach SD, Rustgi AK Tags: Mol Biol Cell Source Type: journals
A Genomic Screen for Yeast Mutants Defective in Selective Mitochondria Autophagy.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: Charles Boone Mitophagy is the process of selective mitochondrial degradation via autophagy, which has an important role in mitochondrial quality control. Very little is known, however, about the molecular mechanism of mitophagy. A genome-wide yeast mutant screen for mitophagy-defective strains identified 32 mutants with a block in mitophagy, in addition to the known autophagy-related (ATG) gene mutants. We further characterized one of these mutants, ylr356wDelta that corresponds to a gene whose function has not been identified. YLR356W is a mitophagy-specific gene that was not required for other typ...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Kanki T, Wang K, Baba M, Bartholomew CR, Lynch-Day MA, Du Z, Geng J, Mao K, Yang Z, Yen WL, Klionsky DJ Tags: Mol Biol Cell Source Type: journals
Heat Shock Factor 1 Controls Genome-wide Acetylation in Heat-shocked Cells.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: A. Gregory Matera A major regulatory function has been evidenced here for HSF1, the key transcription factor of the heat shock response, in a large scale remodeling of the cell epigenome. Indeed, upon heat shock, HSF1, in addition to its well-known transactivating activities, mediates a genome-wide and massive histone deacetylation. Investigating the underlying mechanisms, we show that HSF1 specifically associates with and uses HDAC1 and HDAC2 to trigger this heat-shock dependent histone deacetylation. This work therefore identifies HSF1 as a master regulator of global chromatin acetylation and revea...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Fritah S, Col E, Boyault C, Govin J, Sadoul K, Chiocca S, Christians E, Khochbin S, Jolly C, Vourc'h C Tags: Mol Biol Cell Source Type: journals
Telomeric DNA Mediates De Novo PML Body Formation.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: A. Gregory Matera The cell nucleus harbors a variety of different bodies that vary in number, composition and size. Although these bodies coordinate important nuclear processes little is known about how they are formed. Among the most intensively studied bodies in recent years is the PML body. These bodies have been implicated in gene regulation and other cellular processes and are disrupted in cells from patients suffering from acute promyelocytic leukemia. Using live cell imaging microscopy and immunofluorescence, we show in several cell types that PML bodies are formed at telomeric DNA during inte...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Brouwer AK, Schimmel J, Wiegant JC, Vertegaal AC, Tanke HJ, Dirks RW Tags: Mol Biol Cell Source Type: journals
The Drosophila Kinesin-13, KLP59D, Impacts Pacman and Flux-based Chromosome Movement.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: Yixian Zheng Chromosome movements are linked to the active depolymerization of spindle microtubule (MT) ends. Here we identify the kinesin-13 family member, KLP59D, as a novel and uniquely important regulator of spindle MT dynamics and chromosome motility in Drosophila somatic cells. During prometaphase and metaphase, depletion of KLP59D, which targets to centrosomes and outer kinetochores, suppresses the depolymerization of spindle pole-associated MT minus-ends thereby inhibiting poleward tubulin Flux. Subsequently, during anaphase, loss of KLP59D strongly attenuates chromatid-to-pole motion by supp...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Rath U, Rogers GC, Tan D, Gomez-Ferreria MA, Buster DW, Sosa HJ, Sharp DJ Tags: Mol Biol Cell Source Type: journals
The M Phase Kinase Greatwall (Gwl) Promotes Inactivation of PP2A/B55{delta}, a Phosphatase Directed Against CDK Phosphosites.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: Orna Cohen-Fix We have previously shown that Greatwall kinase (Gwl) is required for M phase entry and maintenance in Xenopus egg extracts. Here, we demonstrate that Gwl plays a crucial role in a novel biochemical pathway that inactivates, specifically during M phase, "anti-mitotic" phosphatases directed against phosphorylations catalyzed by cyclin-dependent kinases (CDKs). A major component of this phosphatase activity is heterotrimeric PP2A containing the B55delta regulatory subunit. Gwl is activated during M phase by Cdk1/Cyclin B (MPF), but once activated, Gwl promotes PP2A/B55delta inhibition wit...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Castilho PV, Williams BC, Mochida S, Zhao Y, Goldberg ML Tags: Mol Biol Cell Source Type: journals
Caspase-independent Mitochondrial Cell Death Results from Loss of Respiration, not Cytotoxic Protein Release.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: Thomas D. Fox In apoptosis, mitochondrial outer membrane permeabilization (MOMP) triggers caspase-dependent death. However, cells undergo clonogenic death even if caspases are blocked. One proposed mechanism involved the release of cytotoxic proteins (e.g., AIF and endoG) from mitochondria. To initiate MOMP directly without side effects, we created a tamoxifen-switchable BimS fusion protein. Surprisingly, even after MOMP, caspase-inhibited cells replicated DNA and divided for approximately 48 h before undergoing proliferation arrest. AIF and endoG remained in mitochondria. However, cells gradually lo...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Lartigue L, Kushnareva Y, Seong Y, Lin H, Faustin B, Newmeyer DD Tags: Mol Biol Cell Source Type: journals
Regulation of the Transcription Factor YY1 in Mitosis through Phosphorylation of Its DNA Binding Domain.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: A. Gregory Matera Yin-Yang 1 (YY1) is a ubiquitously expressed zinc finger transcription factor. It regulates a vast array of genes playing critical roles in development, differentiation, and cell cycle. Very little is known about the mechanisms that regulate the functions of YY1. It has long been proposed that YY1 is a phosphoprotein; however, a direct link between phosphorylation and the function of YY1 has never been proven. Investigation of the localization of YY1 during mitosis shows that it is distributed to the cytoplasm during prophase and remains excluded from DNA until early telophase. Immu...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Rizkallah R, Hurt MM Tags: Mol Biol Cell Source Type: journals
Murine CENP-F Regulates Centrosomal Microtubule Nucleation and Interacts with Hook2 at the Centrosome.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Monitoring Editor: Yixian Zheng The microtubule network (MT) is essential in a broad spectrum of cellular functions. Many studies have linked CENP-F to MT-based activities as disruption of this protein leads to major changes in MT structure and function. Still the basis of CENP-F regulation of the MT network remains elusive. Here, our studies reveal a completely novel and critical localization and role for CENP-F at the centrosome, the major MT organizing center (MOTC) of the cell. Using a yeast two-hybrid screen, we identify Hook2, a linker protein that is essential for regulation of the MT network at the centrosome, ...
Source: Mol Biol Cell - September 29, 2009 Category: Molecular Biology Authors: Moynihan K, Pooley R, Miller P, Kaverina I, Bader D Tags: Mol Biol Cell Source Type: journals
Molecular risk stratification in advanced heart failure patients.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Conclusions: Gene expression profiling has the potential to reproducibly detect heart failure patients with highest heart failure severity with high sensitivity and specificity. In addition, not only LV but also RV samples could be used for molecular risk stratification with similar predictive power.
PMID: 19793385 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - September 29, 2009 Category: Molecular Biology Authors: Lamirault G, Le Meur N, Roussel JC, Le Cunff MF, Baron D, Bihouée A, Guisle I, Raharijaona M, Ramstein G, Teusan R, Chevalier C, Gueffet JP, Trochu JN, Léger JJ, Houlgatte R, Steenman M Tags: J Cell Mol Med Source Type: journals
Two Dicarbonyl compounds, 3-deoxyglucosone and methylglyoxal, differentially modulate dermal fibroblasts.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Advanced glycation endproducts accumulate on long-lived proteins such as collagens as a function of diet and age and mediate the cross-linking of those proteins causing changes in collagen pathophysiology resulting in the disruption of normal collagen matrix remodeling. Two commonly studied advanced glycation endproduct precursors 3-deoxyglucosone and methylglyoxal were investigated for their role in the modification of collagen and on extracellular matrix expression. Fibroblasts cultured on methylglyoxal cross-linked matrices increased the expression of collagen, active TGF-beta1, beta1-integrin, and decreased Smad7; ...
Source: Matrix Biology - September 29, 2009 Category: Molecular Biology Authors: Sassi-Gaha S, Loughlin DT, Kappler F, Schwartz ML, Su B, Tobia AM, Artlett CM Tags: Matrix Biol Source Type: journals
MACC1 — more than metastasis? Facts and predictions about a novel geneEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract We recently identified the metastasis-associated in colon cancer 1 (MACC1) gene by a genome-wide search for differentially
expressed genes in human colon cancer tissues, metastases, and normal tissues. Based on MACC1 expression in primary colon
cancers, which did not present with metastases, our negative and positive prediction for metachronous metastasis was correct
in 80% and 74% of cases, respectively. The 5-year-survival was 80% for MACC1 low expressors, but 15% for individuals who showed
high MACC1 expression in their primary tumors. MACC1 induces migration, invasion and proliferation in cell c...
Source: Journal of Molecular Medicine - September 29, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
LFG: a candidate apoptosis regulatory gene familyEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract The expanding wealth of human, model and other organism’s genomic data has allowed the identification of a distinct gene family
of apoptotic related genes. Most of these genes are currently unannotated or have been subsumed under two questionably related
gene families in the past. For example the transmembrane Bax inhibitor 1 (BI1) motif family has been reported to play a role
in apoptosis and to consist of at least seven mammalian protein genes, GRINA, BI1, Lfg/FAIM2, Ghitm, RESC1/Tmbim1, GAAP/Tmbim4, and Tmbm1b. However, a detailed sequence and phylogenetic analysis shows that only five of these ...
Source: Apoptosis - September 29, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
The mineralization phenotype in Abcc6−/− mice is affected by Ggcx gene deficiency and genetic background—a model for pseudoxanthoma elasticumEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study, we examined the hypothesis that the GGCX gene encoding γ-glutamyl carboxylase may interfere with the mineralization process in Abcc6
−/−
mice. Thus, Abcc6
−/−
and Ggcx
+/− mice were generated on 129S1;C57 and 129S1;129X1;C57 genetic backgrounds, respectively, and backcrossed with C57BL/6J for
five generations. Thus, these strains differ by the 129X1 contribution to the background of the mice. We then generated Abcc6
−/−
;Ggcx
+/+ and Abcc6
−/−
;Ggcx
+/− mice by crossing Abcc6
−/−
and Ggcx
+/− mice. The degree of mineralization of connective capsule of v...
Source: Journal of Molecular Medicine - September 29, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Brain cancer stem cellsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract Cancers comprise heterogeneous cells, ranging from highly proliferative immature precursors to more differentiated cell lineages.
In the last decade, several groups have demonstrated the existence of cancer stem cells in both nonsolid solid tumors, including
some of the brain: glioblastoma multiforme (GBM), medulloblastoma, and ependymoma. These cells, like their normal counterpart
in homologous tissues, are multipotent, undifferentiated, self-sustaining, yet transformed cells. In particular, glioblastoma-stem
like cells (GBSCs) self-renew under clonal conditions and differentiate into neuron- and g...
Source: Journal of Molecular Medicine - September 29, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Effects of upregulated expression of microRNA-16 on biological properties of culture-activated hepatic stellate cellsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study, The
miRNA RNAi technique was used to analyze the effects of miR-16 on biological properties of HSCs in vitro. The lentiviral vector
encoding miR-16 was constructed and transfected. Furthermore, the expression level of miR-16 was measured by real-time PCR.
Cellular growth and proliferation capacity were assayed using the cell counting kit-8 (CCK-8). The apoptosis rate and cell-cycle
distribution were measured by flow cytometry. Cell morphological characteristics were identified by phase-contrast microscopy,
fluorescence microscopy and electron microscopy. The underlying mechanisms related to the changes ...
Source: Apoptosis - September 29, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Death of ouabain-treated renal epithelial cells: evidence for p38 MAPK-mediated Na
i+
/K
i+
-independent signalingEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
This study examined the role of mitogen-activated protein kinases (MAPK) in the death of ouabain-treated REC. Exposure
of C7-MDCK cells that resembled principal cells from canine kidney to 3 μM ouabain led to phosphorylation of p38 without significant
impact on phosphorylation of ERK and JNK MAPK. Maximal increment of p38 phosphorylation was observed at 4 h followed by cell
death at 12 h of ouabain addition. In contrast to ouabain, neither cell death nor p38 MAPK phosphorylation were affected by
elevation of the [Na+]i/[K+]i ratio triggered by Na+,K+-ATPase inhibition in K+-free medium. p38 phosphorylat...
Source: Apoptosis - September 29, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Medical molecular morphology with imaging mass spectrometryEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract Imaging mass spectrometry (IMS) is a two-dimensional mass spectrometry to visualize the spatial distribution of biomolecules
that does not need either separation or purification of target molecules and enables us to monitor not only the identification
of unknown molecules but also the localization of numerous molecules simultaneously. Among the ionization techniques, matrix-assisted
laser desorption/ionization (MALDI) is one of those most generally used for IMS, which allows the analysis of numerous biomolecules
ranging over wide molecular weights. At present, targets of IMS research have expanded t...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
Primitive neuroectodermal tumor of the kidneyEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
We report a 21-year-old man with a primitive neuroectodermal tumor (PNET) of the kidney that was difficult to distinguish
from other small round cell tumors, e.g., small cell carcinoma, malignant lymphoma, and Wilms’ tumor. Pathologically, a primitive
rosette-like pattern was shown by H&E staining; expression of MIC-2 was indicated by an immunohistochemical test; rather primitive
organelles were observed by an ultrastructural method; and translocation of chromosome 22 was confirmed by FISH. We therefore
diagnosed the current case as PNET. The patient had undergone a right radical nephrectomy more than 1.5 years earli...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
Pancreatic-type mixed acinar-endocrine carcinoma with alpha-fetoprotein production arising from the stomach: a report of an extremely rare caseEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract An extremely rare case of mixed acinar-endocrine carcinoma (MAEC) arising from the stomach in a 56-year-old Japanese woman
is herein presented. An endoscopic examination and computed tomography showed a protruding gastric tumor and a large extragastric
mass, respectively. Macroscopic observation on the surgical specimen revealed the extragastric cystic mass was continued to
the intragastric tumor. Histologically, the intragastric tumor consisted of large or small solid nests with acinar appearance.
The cancer cells had an ovoid nuclei and polygonal cytoplasm, which was frequently amphophilic. Immuno...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
Phagocytosis mechanism of apoptotic granulosa cells regulated by milk-fat globule-EGF factor 8Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract In the process of ovary sexual maturation, most immature ovarian follicles degrade into atretic follicles accompanied by apoptosis
in granulosa cells. Macrophages can recognize apoptotic cells through specific binding with phosphatidylserine (PS), exposed
on the surface of apoptotic cells, which is mediated by milk-fat globule-EGF factor 8 (MFG-E8). In the present research, we
examined the involvement of the MFG-E8-dependent phagocytosis system in the atretic follicles of developing mouse ovaries.
The number of atretic follicles and DNA-fragmented granulosa cells significantly increased in B6C3F1 mi...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
The immunohistochemical expression profile of osteopontin in normal human tissues using two site-specific antibodies reveals a wide distribution of positive cells and extensive expression in the central and peripheral nervous systemsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In conclusion, we demonstrate that OPN is
widely distributed in normal human cells, particularly those comprising the central and peripheral nervous systems.
Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-009-0459-6Authors
Yasuto Kunii, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 JapanShin-ichi Niwa, Fukushima Medical University School of Medicine Department of Neuropsychiatry Fukushima JapanYoshiaki Hagiwara, Immunobiological Laboratories Takasaki JapanMasahiro Maeda, Immunobiological Laboratories Takasaki JapanTsutomu Seitoh, Immun...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
Impact of hepatitis B virus X protein on the DNA damage response during hepatocarcinogenesisEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal cancers worldwide. The main HCC-associated diseases
are chronic infections with hepatitis B virus (HBV) and hepatitis C virus (HCV), and HBV-associated HCC is still prevalent
in Asia. Many studies have suggested that HBV X protein (HBX), which is the most common ORF integrated into the host genome,
plays a crucial role in hepatocarcinogenesis. However, the accumulated evidence regarding HBX-mediated signaling pathways
is not concordant, and it is difficult to understand the mechanistic nature of HBX-associated hepatocarcinogenesi...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
Extraskeletal myxoid chondrosarcoma: a study using a quick-freezing and deep-etching methodEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract A case of extraskeletal myxoid chondrosarcoma (ESMC), which developed in the right thigh of a middle-aged Japanese woman,
was studied using immunohistochemistry, conventional electron microscopy, and the quick-freezing and deep-etching (QF-DE)
method. In addition to typical light microscopic findings of ESMC, conventional electron microscopy indicated that the tumor
cells had features of chondrocytes. Immunohistochemically, the tumor cells showed a positive immunoreaction for S100 protein.
A diagnosis of ESMC was made. An interesting observation was the ultrastructural features of collagen fibrils i...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
Clear cell chondrosarcoma: an ultrastructural studyEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
We report here a case of CCC. A 67-year-old Japanese man presented with
right arthralgia for 1 year, and histological examination of the subsequent surgical resection of the right femoral bone showed
the finding of CCC. Ultrastructurally, most organelles were observed in the perinuclear area. Clear neoplastic cells contained
many glycogen particles in the area of the cytoplasm lacking organelles, although glycogen particles overall seemed to be
evenly distributed in the cytoplasm. Some mitochondria, Golgi complex, actin-like filaments, and rough endoplasmic reticulum
were also demonstrated in the cytoplasm of clear ce...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
Immunohistochemical and electron microscopy studies of a case of hyalinizing trabecular tumor of the thyroid gland, with special consideration of the hyalinizing mass associated with itEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
We report here a case of HTT with cytological, immunohistochemical, and ultrastructural
findings, focused in particular on the hyalinizing mass. Cytologically, tumor cells exhibiting many intranuclear cytoplasmic
inclusions and nuclear grooves were found in association with light green-positive, irregular, fluffy membranous structures
on touch smear. Staining with antibody to collagen type IV was positive in these membranous structures. Histopathologically,
tumor cells exhibited many intranuclear cytoplasmic inclusions, and were positive for staining with antibodies to S100 protein,
neuron-specific enolase, thyroglobu...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
Morphometric analysis of regional lymph nodes in surgically resected non-small cell lung cancerEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract Nodal staging is a crucial factor in choosing the treatment option for non-small cell lung cancer (NSCLC). However, so far
as we know, a computer-based histomorphometric analysis of lymph nodes in NSCLC has never been developed. We studied 299 surgically
resected lymph nodes from 108 patients with NSCLC. Microscopic digital images were analyzed with Scion Image software. Seventy
lymph nodes had at least one metastatic focus. The metastasis occupancy area per node ranged from 0.01 to 209.58 mm2 (mean, 29.58 ± 5.87 mm2). The metastasis occupancy ratio ranged from 0.01% to 100% (mean, 48.70% ± 42.10%)...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
Environmental factors involved in axonal regeneration following spinal cord transection in ratsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Abstract A recent study of a rat model treated with grafted collagen filament (CF) after spinal cord transection showed dramatic recovery
of motor function but did not report on the acute-stage phenomenon. In the present study, we describe molecular and histological
aspects of the axonal regeneration process during the acute stage following spinal cord transection. The spinal cord of 8-week-old
rats was completely transected, and a scaffold of almost the same size as the resected portion was implanted in the gap. Changes
in the mRNA expression of four neurotrophic factors [nerve growth factor (NGF), brain-de...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals
[11C]Gefitinib ([11C]Iressa): Radiosynthesis, In Vitro Uptake, and In Vivo Imaging of Intact Murine FibrosarcomaEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Conclusion These results demonstrated the promising potential of [11C]gefitinib to serve as a PET ligand for in vivo imaging of NFSa-bearing mice.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0265-5Authors
Ming-Rong Zhang, National Institute of Radiological Sciences Department of Molecular Probes, Molecular Imaging Center 4-9-1 Anagawa, Inage-ku Chiba JapanKatsushi Kumata, National Institute of Radiological Sciences Department of Molecular Probes, Molecular Imaging Center 4-9-1 Anagawa, Inage-ku Chiba JapanAkiko Hatori, National Institute of Radiological Sciences Departmen...
Source: Molecular Imaging and Biology - September 29, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
Comparison of the interactomic networks of different species in terms of accessibilityEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Francisco A. Rodrigues, Luciano da Fontoura Costa
(Paper from Mol. BioSyst.)
Francisco A. Rodrigues, Mol. BioSyst., 2009, DOI: 10.1039/b906966f
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 28, 2009 Category: Molecular Biology Authors: Francisco A. RodriguesLuciano da Fontoura Costa Source Type: journals
Rapid identification of orexin receptor binding ligands using cell-based screening accelerated with magnetic beadsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Xin Qi, John Astle, Thomas Kodadek
(Method from Mol. BioSyst.)
Xin Qi, Mol. BioSyst., 2010, DOI: 10.1039/b915611a
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 28, 2009 Category: Molecular Biology Authors: Xin QiJohn Astle Thomas Kodadek Source Type: journals
In vivo proton HR-MAS NMR metabolic profile of the freshwater cladoceran Daphnia magnaEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Andrei Bunescu, Jeanne Garric, Bernard Vollat, Emmanuelle Canet-Soulas, Danielle Graveron-Demilly, Florence Fauvelle
(Method from Mol. BioSyst.)
Andrei Bunescu, Mol. BioSyst., 2010, DOI: 10.1039/b915417e
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 28, 2009 Category: Molecular Biology Authors: Andrei BunescuJeanne Garric Bernard Vollat Emmanuelle Canet-Soulas Danielle Graveron-Demilly Florence Fauvelle Source Type: journals
Structural and functional characteristics of xenavidin, the first frog avidin from Xenopus tropicalisEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Conclusions:
The novel data reported here provide information about the biochemically and structurally important determinants of biotin binding. This information may facilitate the discovery of novel tools for biotechnology.
Source: BMC Structural Biology - Latest articles - September 28, 2009 Category: Molecular Biology Authors: Juha MaattaSatu HelppolainenVesa HytonenMark JohnsonMarkku KulomaaTomi AirenneHenri Nordlund Source Type: journals
Involvement of S-nitrosylation of actin in inhibition of neurotransmitter release by nitric oxideEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Conclusions:
The present study demonstrates that actin is a major S-nitrosylated protein in the spinal cord and suggests that NO directly regulates neurotransmitter release by S-nitrosylation in addition to the well-known phosphorylation by cGMP-dependent protein kinase.
Source: Molecular Pain - September 28, 2009 Category: Molecular Biology Authors: Jingshan LuTayo KatanoEmiko Okuda-AshitakaYo OishiYoshihiro UradeSeiji Ito Source Type: journals
Cytogenetic genotoxicity of amoxicillinEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study, AMO did not induce SCEs or CAs in human peripheral blood lymphocytes both in the presence and absence of the metabolic activator. AMO concentration-dependently decreased the proliferation index (PI) in the absence of the metabolic activation for 24-hr treatment period. Mitotic index (MI) was generally found to have been reduced when compared with the negative control but not with the solvent control in cultures treated with AMO for 24 hr. AMO did not decrease the PI and MI in the presence of the metabolic activator. Furthermore, AMO neither induced the formation of MN nor decreased the nuclear division index...
Source: Environmental and Molecular Mutagenesis - September 28, 2009 Category: Molecular Biology Authors: Erman Salih [Idot]stifli, Mehmet Topakta[scedil] Source Type: journals
Flow cytometric scoring of micronucleated reticulocytes as a possible high-throughput radiation biodosimeterEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Micronucleated reticulocyte (MN-RET) scoring by flow cytometry (FCM) has been used in assessment of the clastogenic effects of chemicals. However, its dose-response to acute whole body irradiation (WBI) at moderate dose rates remains to be established. We show that FCM scoring of MN-RET in peripheral blood from male ICR mice exposed to WBI X-ray doses of 0.5-5 Gy at a dose rate of 0.488 Gy/min exhibits a linear dose-response relationship 24, 48, and 72 hr following WBI. Parallel microscopic counting of micronucleated polychromatic erythrocytes (MN-PCE) in bone marrow smears from the same animals showed similar linear dose-...
Source: Environmental and Molecular Mutagenesis - September 28, 2009 Category: Molecular Biology Authors: Libo Liu, Yuguang Liu, Guangying Ni, Shuzheng Liu Source Type: journals
TSG101, identified by screening a cancer cDNA library and soft agar assay, promotes cell proliferation in human lung cancer.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Understanding the genesis and development of tumors is an essential component in cancer research. It is of interest to discover unknown genes that are responsible for cellular transformation. A cDNA library of a highly metastatic lung adenocarcinoma cell line was constructed. This library was introduced into the NIH3T3 mouse embryonic fibroblast cell line to screen for cDNAs that increase anchorage-independent colony formation in soft agar. The expression of TSG101 in lung cancer cell lines and specimens was confirmed using reverse transcription-polymerase chain reaction. The level of TSG101 protein in transfected A549...
Source: Molecular Biology Reports - September 28, 2009 Category: Molecular Biology Authors: Liu F, Yu Y, Jin Y, Fu S Tags: Mol Biol Rep Source Type: journals
Influences of genetic variants in interleukin-15 gene and serum interleukin-15 levels on coronary heart disease.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In conclusion, our study supports the hypothesis that genetic variation in IL-15 gene and IL-15 levels influence the risk of CHD. Further studies are needed to confirm our hypothesis.
PMID: 19796965 [PubMed - as supplied by publisher]
Source: Cytokine - September 28, 2009 Category: Molecular Biology Authors: Gokkusu C, Aydin M, Ozkok E, Tulubas F, Elitok A, Pamukcu B, Umman B Tags: Cytokine Source Type: journals
Identification of alpha-dystroglycan binding sequences in the laminin alpha2 chain LG4-5 module.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study, heparin and alpha-dystroglycan binding sequences were identified using 42 overlapping synthetic peptides from the LG4-5 module and using recombinant LG4-5 protein (rec-alpha2LG4-5). Physiological activities of the active peptides were also examined in explants of submandibular glands. Heparin binding screens showed that the A2G78 peptide (GLLFYMARINHA) bound to heparin and prevented its binding to rec-alpha2LG4-5. Furthermore, alanine substitution of the arginine residue in the A2G78 site on rec-alpha2LG4-5 decreased heparin binding activity. When alpha-dystroglycan binding of the peptides was screened, two ...
Source: Matrix Biology - September 28, 2009 Category: Molecular Biology Authors: Suzuki N, Hozumi K, Urushibata S, Yoshimura T, Kikkawa Y, Gumerson JD, Michele DE, Hoffman MP, Yamada Y, Nomizu M Tags: Matrix Biol Source Type: journals
Bioinformatics in bioinorganic chemistryEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Ivano Bertini, Gabriele Cavallaro
(From Metallomics)
Ivano Bertini, Metallomics, 2010, DOI: 10.1039/b912156k
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chemical Biology Virtual Journal latest articles - September 28, 2009 Category: Molecular Biology Authors: Ivano BertiniGabriele Cavallaro Source Type: journals
Preventing autoimmunity by regulating regulatory T-cell inductionEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Content Type Journal ArticleCategory Clinical ImplicationsDOI 10.1007/s00109-009-0536-2Authors
Friedrich Luft, Experimental and Clinical Research Center Berlin-Buch Germany
Journal Journal of Molecular MedicineOnline ISSN 1432-1440Print ISSN 0946-2716
Source: Journal of Molecular Medicine - September 28, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Artificial assembly of a minimal cellEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Giovanni Murtas
(Review from Mol. BioSyst.)
Giovanni Murtas, Mol. BioSyst., 2009, DOI: 10.1039/b906541e
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 27, 2009 Category: Molecular Biology Authors: Giovanni Murtas Source Type: journals
Predicting protein-protein interactions in the context of protein evolutionEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Anna C. F. Lewis, Ramazan Saeed, Charlotte M. Deane
(Review from Mol. BioSyst.)
Anna C. F. Lewis, Mol. BioSyst., 2010, DOI: 10.1039/b916371a
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 27, 2009 Category: Molecular Biology Authors: Anna C. F. LewisRamazan Saeed Charlotte M. Deane Source Type: journals
The influence of EDTA and citrate anticoagulant addition to human plasma on information recovery from NMR-based metabolic profiling studiesEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Richard H. Barton, Daniel Waterman, Frank W. Bonner, Elaine Holmes, Robert Clarke, the PROCARDIS Consortium, Jeremy K. Nicholson, John C. Lindon
(Paper from Mol. BioSyst.)
Richard H. Barton, Mol. BioSyst., 2010, DOI: 10.1039/b907021d
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 27, 2009 Category: Molecular Biology Authors: Richard H. BartonDaniel Waterman Frank W. Bonner Elaine Holmes Robert Clarke the PROCARDIS Consortium Jeremy K. Nicholson John C. Lindon Source Type: journals
Prediction of protein-protein interactions between Helicobacter pylori and a human hostEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Nidhi Tyagi, Oruganty Krishnadev, Narayanaswamy Srinivasan
(Paper from Mol. BioSyst.)
Nidhi Tyagi, Mol. BioSyst., 2009, DOI: 10.1039/b906543c
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 27, 2009 Category: Molecular Biology Authors: Nidhi TyagiOruganty Krishnadev Narayanaswamy Srinivasan Source Type: journals
Synthesis of acetal protected building blocks using flow chemistry with flow I.R. analysis: preparation of butane-2,3-diacetal tartratesEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Catherine F. Carter, Ian R. Baxendale, Matthew O'Brien, John B. J. Pavey, Steven V. Ley
(Communication from Org. Biomol. Chem.)
Catherine F. Carter, Org. Biomol. Chem., 2009, DOI: 10.1039/b917289k
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - September 27, 2009 Category: Molecular Biology Authors: Catherine F. CarterIan R. Baxendale Matthew O'Brien John B. J. Pavey Steven V. Ley Source Type: journals
Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCREmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Conclusion:
None of the candidate reference genes was uniformly expressed across all experimental conditions, and the most suitable reference genes are conditional-, tissue-specific-, developmental-, and cultivar-dependent. Most of the new reference genes performed better than the conventional housekeeping genes. These results should guide the selection of reference genes for gene expression studies in soybean.
Source: BMC Molecular Biology - Latest articles - September 27, 2009 Category: Molecular Biology Authors: Ruibo HuChengming FanHongyu LiQingzhu ZhangYong-Fu Fu Source Type: journals
Identification of hemagglutinin structural domain and polymorphisms which may modulate swine H1N1 interactions with human receptorEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Conclusions:
Our results (i) contribute to better understanding of the origin of the novel A/H1N1 influenza virus, (ii) provide a tool for monitoring its molecular evolution (iii) predicts hotspots associated with enhanced infectivity in humans and (iv) identify therapeutic and diagnostic targets for prevention and treatment of A/H1N1 infection.
Source: BMC Structural Biology - Latest articles - September 27, 2009 Category: Molecular Biology Authors: Veljko VeljkovicHenry NimanSanja GlisicNevena VeljkovicVladimir PerovicClaude Muller Source Type: journals
