Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 21.
Using FAM labeled DNA oligos to do RNA electrophoretic mobility shift assay.
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We present here the use of fluorescent methodologies for studies of RNA in place of radioactivity. The method is highly sensitive and quantitative with the use of an infrared fluorescence imaging system. Fluorescently labeled primers can be used to monitor protein-RNA interactions by fluorescent mobility shift assays. The simplicity and validity of this approach may have more advantages than that of previous methods that traditionally used hazardous radioisotopes. This method was successfully tested in the study of the interactions between MS2 Coat Protein and its RNA target.
PMID: 19784797 [PubMed - as supplied by publisher]
Source: Molecular Biology Reports - September 25, 2009 Category: Molecular Biology Authors: Wang K, Gao Y, Peng X, Yang G, Gao F, Li S, Zhu Y Tags: Mol Biol Rep Source Type: journals
Myeloperoxidase-derived oxidants selectively disrupt the protein core of the heparan sulfate proteoglycan perlecan.Email this article to a colleague.
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The potent oxidants hypochlorous acid (HOCl) and hypobromous acid (HOBr) are produced extracellularly by myeloperoxidase, following release of this enzyme from activated leukocytes. The subendothelial extracellular matrix is a key site for deposition of myeloperoxidase and damage by myeloperoxidase-derived oxidants, with this damage implicated in the impairment of vascular cell function during acute inflammatory responses and chronic inflammatory diseases such as atherosclerosis. The heparan sulfate proteoglycan perlecan, a key component of the subendothelial extracellular matrix, regulates important cellular processes...
Source: Matrix Biology - September 25, 2009 Category: Molecular Biology Authors: Rees MD, Whitelock JM, Malle E, Chuang CY, Iozzo RV, Nilasaroya A, Davies MJ Tags: Matrix Biol Source Type: journals
Can we intervene in human ageing?Email this article to a colleague.
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Review ArticlesRichard G.A. Faragher, Angela N. Sheerin, Elizabeth L. Ostler, Expert Reviews in Molecular Medicine, Volume 11 , pp e27AbstractAgeing is a progressive failure of defence and repair processes that produces physiological frailty (the loss of organ reserve with age), loss of homeostasis and eventual death. Over the past ten years exceptional progress has been made in understanding both why the ageing process happens and the mechanisms that are responsible for it. The study of natural mutants that accelerate some, but not all, of the features of the human ageing process has now progressed to a degree that drug t...
Source: Expert Reviews in Molecular Medicine - September 25, 2009 Category: Molecular Biology Source Type: journals
Development and Validation of a Monte Carlo Simulation Tool for Multi-Pinhole SPECTEmail this article to a colleague.
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Conclusions The new method is useful for studying MPH collimator designs, data acquisition protocols, image reconstructions, and compensation
techniques. It also has great potential to be applied for modeling the collimator-detector response with penetration and scatter
effects for MPH in the quantitative reconstruction method.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0263-7Authors
Greta S. P. Mok, The Chinese University of Hong Kong Department of Diagnostic Radiology and Organ Imaging Shatin N.T. Hong KongYong Du, Johns Hopkins University Division of Medical Imaging...
Source: Molecular Imaging and Biology - September 25, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
64Cu-Labeled Affibody Molecules for Imaging of HER2 Expressing TumorsEmail this article to a colleague.
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This study demonstrates that 64Cu-labeled ZHER2:477 is a promising targeted molecular probe for imaging HER2 receptor expression in living mice. Further work is needed to improve
the excretion properties, hence dosimetry and imaging efficacy, of the radiometal-based probe.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0256-6Authors
Zhen Cheng, Stanford University Molecular Imaging Program at Stanford (MIPS), Departments of Radiology and Bioengineering, Bio-X Program California CA 94305-5344 USAOmayra Padilla De Jesus, Global Research Center General Electric Company Niskayuna NY 12309...
Source: Molecular Imaging and Biology - September 25, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
Effect of clustered peptide binding on DNA condensationEmail this article to a colleague.
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Jennifer Haley, Paul Kabiru, Yan Geng
(Paper from Mol. BioSyst.)
Jennifer Haley, Mol. BioSyst., 2010, DOI: 10.1039/b908873c
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Source: RSC - Mol. BioSyst. latest articles - September 24, 2009 Category: Molecular Biology Authors: Jennifer HaleyPaul Kabiru Yan Geng Source Type: journals
Biomolecule detection with peroxidase-mimicking DNAzymes; expanding detection modality with fluorogenic compoundsEmail this article to a colleague.
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Shizuka Nakayama, Herman O. Sintim
(Communication from Mol. BioSyst.)
Shizuka Nakayama, Mol. BioSyst., 2009, DOI: 10.1039/b916228c
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Source: RSC - Mol. BioSyst. latest articles - September 24, 2009 Category: Molecular Biology Authors: Shizuka NakayamaHerman O. Sintim Source Type: journals
Dynamical effects of epigenetic silencing of 14-3-3[sigma] expressionEmail this article to a colleague.
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Julio Vera, Julia Schultz, Saleh Ibrahim, Yvonne Raatz, Olaf Wolkenhauer, Manfred Kunz
(Paper from Mol. BioSyst.)
Julio Vera, Mol. BioSyst., 2009, DOI: 10.1039/b907863k
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Source: RSC - Mol. BioSyst. latest articles - September 24, 2009 Category: Molecular Biology Authors: Julio VeraJulia Schultz Saleh Ibrahim Yvonne Raatz Olaf Wolkenhauer Manfred Kunz Source Type: journals
Immature and mature species of the human Prostacyclin Receptor are ubiquitinated and targeted to the 26S proteasomal or lysosomal degradation pathways, respectivelyEmail this article to a colleague.
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Conclusion:
These findings indicate that the hIP is post-translationally modified by ubiquitination, which targets the immature species to the 26S proteasomal degradation pathway and the mature species to the lysosomal degradation pathway.
Source: Journal of Molecular Signaling - September 24, 2009 Category: Molecular Biology Authors: Peter DonnellanB Therese Kinsella Source Type: journals
An EST screen from the annelid Pomatoceros lamarckii reveals patterns of gene loss and gain in animalsEmail this article to a colleague.
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Conclusions:
The molecular phylogenetic trees including the P. lamarckii sequences extend early observations that polychaetes tend to have relatively short branches in such trees, and hence are useful taxa with which to reconstruct gene family evolution. Also, with the availability of lophotrochozoan data such as that of P. lamarckii, it is now possible to make much more accurate reconstructions of the gene complement of the ancestor of the bilaterians than was previously possible from comparisons of ecdysozoan and deuterostome genomes to non-bilaterian outgroups. It is clear that the traditional molecular model systems fo...
Source: BMC Evolutionary Biology - Latest articles - September 24, 2009 Category: Molecular Biology Authors: Tokiharu TakahashiCarmel McDougallJolyon TrosciankoWei-Chung ChenAhamarshan Jayaraman-NagarajanSebastian ShimeldDavid Ferrier Source Type: journals
Single and multiple CH (calponin homology) domain containing multidomain proteins in Dictyostelium discoideum: an inventory.Email this article to a colleague.
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We present an inventory of single or multiple calponin homology (CH) domain containing proteins of Dictyostelium discoideum. A multiple alignment and a phylogenetic tree of all 60 CH domains found in 36 proteins showed that most CH domains can be assigned to one of 6 types. We have then distributed the proteins into several classes according to the type and arrangement of the CH domains. Most proteins belong to the class of ABD (actin-binding domain)-forming CH tandems (CH1-CH2) of the alpha-actinin and fimbrin families or to the class of CH3 domain-bearing proteins. There are a few examples of proteins with a single CH1 o...
Source: Molecular Biology Reports - September 24, 2009 Category: Molecular Biology Authors: Friedberg F, Rivero F Tags: Mol Biol Rep Source Type: journals
Matricellular proteins: an overview.Email this article to a colleague.
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PMID: 19779848 [PubMed - as supplied by publisher]
Source: Journal of Cell Communication and Signaling - September 24, 2009 Category: Molecular Biology Authors: Bornstein P Tags: J Cell Commun Signal Source Type: journals
Human Ghrelin Ameliorate Organ Injury and Improves Survival after Radiation Injury Combined with Severe Sepsis.Email this article to a colleague.
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In conclusion, human ghrelin is beneficial in a rat model of RCI. The protective effect of human ghrelin appears to be attributed to re-balancing the dysregulated sympathetic/parasympathetic nervous systems.
PMID: 19779631 [PubMed - as supplied by publisher]
Source: Molecular Medicine - September 24, 2009 Category: Molecular Biology Authors: Shah KG, Wu R, Jacob A, Blau SA, Ji Y, Dong W, Marini CP, Ravikumar TS, Cappa GF, Wang P Tags: Mol Med Source Type: journals
Bladder cancer associated protein: a potential prognostic biomarker in human bladder cancer.Email this article to a colleague.
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It is becoming increasingly clear that no single marker will have the sensitivity and specificity necessary to be used on its own for diagnosis/prognosis of tumors. Inter-patient and intra-tumor heterogeneity provide overwhelming odds against the existence of such an ideal marker. With this in mind our laboratory has been applying a long-term systematic approach to identify multiple biomarkers that can be used for clinical purposes. As a result of these studies, we have identified and reported several candidate biomarker proteins that are deregulated in bladder cancer. Following the conceptual biomarker development pha...
Source: Molecular and Cellular Proteomics : MCP - September 24, 2009 Category: Molecular Biology Authors: Moreira JM, Ohlsson G, Gromov P, Simon R, Sauter G, Celis JE, Gromova I Tags: Mol Cell Proteomics Source Type: journals
Cancer cell apoptotic pathways mediated by PEDF: prospects for therapy.Email this article to a colleague.
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Pigment epithelium-derived factor (PEDF) has roles in antiangiogenesis and antitumourigenesis that are intimately entwined and is showing promise as a potential anticancer agent. However, the function of PEDF in the deregulated apoptotic pathways of malignant cells must first be fully characterized. Here, we review the currently known apoptotic pathways that are relevant to PEDF and cancer. Recently, a pathway that includes the PEDF receptor, PPARgamma and p53 has emerged. It is hoped that further characterization of this and other pathways involved in cancer will bring to light potential new therapeutic targets and ap...
Source: Trends in Molecular Medicine - September 24, 2009 Category: Molecular Biology Authors: Broadhead ML, Dass CR, Choong PF Tags: Trends Mol Med Source Type: journals
Mid-trimester maternal plasma cytokines and CRP as predictors of spontaneous preterm birth.Email this article to a colleague.
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Most previous studies of maternal cytokines and preterm birth have analyzed immunologic biomarkers after the onset of labor or membrane rupture; fewer have examined the systemic (blood) immune response prior to labor onset. We carried out a case-control study nested in a large (n=5337) prospective, multi-center cohort. Cohort women had an interview, examination, and venipuncture at 24-26weeks. Frozen plasma samples in women with spontaneous preterm birth (n=207) and approximately 2 term controls per case (n=444) were analyzed using Luminex multianalyte profiling technology. Fresh placentas were fixed, stained, and blin...
Source: Cytokine - September 24, 2009 Category: Molecular Biology Authors: Kramer MS, Kahn SR, Platt RW, Genest J, Chen MF, Goulet L, Séguin L, Lydon J, McNamara H, Libman M, Dahhou M, Lamoureux J, Skogstrand K, Thorsen P Tags: Cytokine Source Type: journals
DNA Double Strand Breaks by Asbestos, Silica and Titanium dioxide: Possible Biomarker of Carcinogenic Potential?Email this article to a colleague.
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DNA double strand breaks (DNA DSBs) are a very rapid response to DNA damage that occurs in cells subjected to radiation, exposure to toxic substances, and other environmental stresses. The inability to repair these breaks can lead to carcinogenesis. One of the earliest responses to DNA DSBs is the phosphorylation of a histone, H2AX, at serine 139, yielding a focal product (gamma-H2AX) that can be detected by a fluorescent antibody. A study was undertaken to compare the induction of DNA DSBs in normal (SAE) and cancer cells (A549) after exposure to asbestos (crocidolite), a proven carcinogen, silica a suspected carcinog...
Source: American Journal of Respiratory Cell and Molecular Biology - September 24, 2009 Category: Molecular Biology Authors: Msiska Z, Pacurari M, Mishra A, Leonard SS, Castranova V, Vallyathan V Tags: Am J Respir Cell Mol Biol Source Type: journals
Involvement of IL-13 in Tobacco Smoke Induced Changes in the Structure and Function of Rat Intrapulmonary Airways.Email this article to a colleague.
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Chronic obstructive pulmonary disease (COPD) involves disease of small airways with an increase in airway smooth muscle sensitivity to spasmogens and with structural changes described as airway remodeling. We have investigated the effect tobacco smoke (TS) exposure on the structure and function of small airways in rats, and have also studied the role of interleukin (IL)-13 in this response. Precision-cut lung slices (230-280 microm) were prepared from male Sprague-Dawley rats after acute (3 days) or chronic (8 or 16 weeks) daily exposure to TS, or air. Carbachol (CCh) and 5-hydroxytryptamine (5HT) concentration-respons...
Source: American Journal of Respiratory Cell and Molecular Biology - September 24, 2009 Category: Molecular Biology Authors: Cooper PR, Poll CT, Barnes PJ, Sturton RG Tags: Am J Respir Cell Mol Biol Source Type: journals
Rhinovirus-induced Exacerbations of Asthma - How is the {beta}2-adrenoceptor Implicated?Email this article to a colleague.
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Conclusions: This study shows that epithelial infection with RV induces a decrease of ss2AR function on airway smooth muscle cells, potentially explaining the clinical observation of loss of ss2AR agonist function during RV-induced asthma exacerbations.
PMID: 19783788 [PubMed - as supplied by publisher]
Source: American Journal of Respiratory Cell and Molecular Biology - September 24, 2009 Category: Molecular Biology Authors: Trian T, Ge Q, Moir LM, Burgess JK, Kuo C, King NJ, Reddel HK, Black JL, Oliver BG, McParland BE Tags: Am J Respir Cell Mol Biol Source Type: journals
Characterization of PCEng2 a {beta}-1,3-Endoglucanase Homologue in Pneumocystis carinii with Activity in Cell Wall Regulation.Email this article to a colleague.
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In this study we have identified a Pneumocystis beta-1,3-endoglucanase gene (PCEng2) that is demonstrated to play a significant role in cell wall regulation. The full sequence of the gene revealed a 2.2kb open reading frame with conserved amino acid domains homologous to similar fungal glycosyl hydrolases (GH family 81). The gene transcript showed upregulation in cystic isolates and the expressed protein was detected within both cyst and trophic forms. Complementation assays in Eng2 deleted S. cerevisiae strains showed restoration of the cell wall separation defect during proliferation, demonstrating the importance of PCEn...
Source: American Journal of Respiratory Cell and Molecular Biology - September 24, 2009 Category: Molecular Biology Authors: Villegas LR, Kottom TJ, Limper AH Tags: Am J Respir Cell Mol Biol Source Type: journals
Human Rhinovirus Infection Upregulates MMP-9 Production in Airway Epithelial Cells via NF-{kappa}B.Email this article to a colleague.
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In this study we sought, firstly, to determine whether HRV infection modulated the expression of MMP-9 and its highest affinity inhibitor, tissue inhibitor of metalloproteinase (TIMP)-1 and, secondly, to explore the mechanism by which this occurs. In vitro studies, using RT-PCR, ELISA, zymography and a fluorescent activity assay, demonstrated that MMP-9 mRNA, protein and activity were increased upon infection with HRV, while TIMP-1 mRNA and protein remained unchanged. These results were then verified in vivo, using nasal lavage samples obtained from subjects with confirmed rhinovirus infections. HRV infections have been sh...
Source: American Journal of Respiratory Cell and Molecular Biology - September 24, 2009 Category: Molecular Biology Authors: Tacon CE, Wiehler S, Holden NS, Newton R, Proud D, Leigh R Tags: Am J Respir Cell Mol Biol Source Type: journals
The orf virus inhibitor of apoptosis functions in a Bcl-2-like manner, binding and neutralizing a set of BH3-only proteins and active BaxEmail this article to a colleague.
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Abstract We have previously shown that the Orf virus protein, ORFV125, is a potent inhibitor of the mitochondrial pathway of apoptosis
and displays rudimentary sequence similarities to cellular anti-apoptotic Bcl-2 proteins. Here we investigate the proposal
that ORFV125 acts in a Bcl-2-like manner to inhibit apoptosis. We show that the viral protein interacted with a range of BH3-only
proteins (Bik, Puma, DP5, Noxa and all 3 isoforms of Bim) and neutralized their pro-apoptotic activity. In addition, ORFV125
bound to the active, but not the inactive, form of Bax, and reduced the formation of Bax dimers. Mutat...
Source: Apoptosis - September 24, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Capillary arterialization requires the bone marrow-derived cell (BMC)-specific expression of chemokine (C-C motif) receptor-2, but BMCs do not transdifferentiate into microvascular smooth muscleEmail this article to a colleague.
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Abstract Chemokine (C-C motif) receptor-2 (CCR2) regulates arteriogenesis and angiogenesis, facilitating the MCP-1-dependent recruitment
of growth factor-secreting bone marrow-derived cells (BMCs). Here, we tested the hypothesis that the BMC-specific expression
of CCR2 is also required for new arteriole formation via capillary arterialization. Following non-ischemic saphenous artery
occlusion, we measured the following in gracilis muscles: monocyte chemotactic protein-1 (MCP-1) in wild-type (WT) C57Bl/6J
mice by ELISA, and capillary arterialization in WT–WT and CCR2−/−–WT (donor–host) bone marrow c...
Source: Angiogenesis - September 24, 2009 Category: Molecular Biology Tags: Angiogenesis Source Type: journals
Rotenone induces apoptosis in MCF-7 human breast cancer cell-mediated ROS through JNK and p38 signalingEmail this article to a colleague.
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Rotenone is an inhibitor of the mitochondrial electron transport chain complex I, resulting in the generation of reactive oxygen species (ROS). Rotenone has been shown to display anticancer activity through the induction of apoptosis in various cancer cells. However, the underlying mechanism is still not fully understood. Here, rotenone showed a strong growth inhibitory effect against human breast cancer MCF-7 cells. DNA flow cytometric analysis, chromatin condensation, and poly (ADP-ribose) polymerase (PARP) cleavage indicated rotenone actively induced apoptosis in MCF-7 cells. The antiapoptotic protein, Bcl-2, was decrea...
Source: Molecular Carcinogenesis - September 23, 2009 Category: Molecular Biology Authors: Yea-Tzy Deng, Hsiu-Chen Huang, Jen-Kun Lin Source Type: journals
Green fiber lasers: An alternative to traditional DPSS green lasers for flow cytometryEmail this article to a colleague.
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Green and yellow diode-pumped solid-state (DPSS) lasers (532 and 561 nm) have become common fixtures on flow cytometers, due to their efficient excitation of phycoerythrin (PE) and its tandems, and their ability to excite an expanding array of expressible red fluorescent proteins. Nevertheless, they have some disadvantages. DPSS 532-nm lasers emit very close to the fluorescein bandwidth, necessitating optical modifications to permit detection of fluorescein and GFP. DPSS 561-nm lasers likewise emit very close to the PE detection bandwidth and also cause unwanted excitation of APC and its tandems, requiring high levels of c...
Source: Cytometry Part A - September 23, 2009 Category: Molecular Biology Authors: William G. Telford, Sergey A. Babin, Serge V. Khorev, Stephen H. Rowe Source Type: journals
Utility of lyophilized PMA and ionomycin to stimulate lymphocytes in whole blood for immunological assaysEmail this article to a colleague.
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The need to implement robust biomarkers in clinical trials has never been greater, and such efforts can be easily compromised by reagent instability or simple human error during assay set-up. Many biotechnology and pharmaceutical companies are introducing efforts to conduct biomarker studies under more rigorous settings, and the use of plates or tubes pre-loaded with stimulation or staining reagents could be of value for studies that involve flow cytometry.Five reagents lyophilized from ethanol or CHAPS buffer stock solution of phorbol 12-myristate 13-acetate (PMA) and ionomycin were benchmarked against standard DMSO liqui...
Source: Cytometry Part B: Clinical Cytometry - September 23, 2009 Category: Molecular Biology Authors: Shelley Sims Belouski, Julie Wilkinson, John Thomas, Keith Kelley, Shen-Wu Wang, Sid Suggs, John Ferbas Source Type: journals
The ABC of reverse engineering biological signalling systemsEmail this article to a colleague.
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Maria Secrier, Tina Toni, Michael P. H. Stumpf
(Paper from Mol. BioSyst.)
Maria Secrier, Mol. BioSyst., 2009, DOI: 10.1039/b908951a
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Source: RSC - Mol. BioSyst. latest articles - September 23, 2009 Category: Molecular Biology Authors: Maria SecrierTina Toni Michael P. H. Stumpf Source Type: journals
Synthesis, photolysis studies and in vitro photorelease of caged TRPV1 agonists and antagonistsEmail this article to a colleague.
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Michael P. Van Ryssen, Nicolaos Avlonitis, Rashid Giniatullin, Craig McDougall, James L. Carr, Megan N. Stanton-Humphreys, Emma L. A. Borgstrom, C. Tom A. Brown, Dmitriy Fayuk, Alexander Surin, Minna Niittykoski, Leonard Khiroug, Stuart J. Conway
(Paper from Org. Biomol. Chem.)
Michael P. Van Ryssen, Org. Biomol. Chem., 2009, DOI: 10.1039/b914981c
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Source: RSC - Organic Biomolecular Chemistry - September 23, 2009 Category: Molecular Biology Authors: Michael P. Van RyssenNicolaos Avlonitis Rashid Giniatullin Craig McDougall James L. Carr Megan N. Stanton-Humphreys Emma L. A. Borgstrom C. Tom A. Brown Dmitriy Fayuk Alexander Surin Minna Niittykoski Leonard Khiroug Stuart J. Conway Source Type: journals
Synthesis of new, UV-photoactive dansyl derivatives for flow cytometric studies on bile acid uptakeEmail this article to a colleague.
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Jana Rohacova, M. Luisa Marin, Alicia Martinez-Romero, Jose-Enrique O'Connor, M. Jose Gomez-Lechon, M. Teresa Donato, Jose V. Castell, Miguel A. Miranda
(Paper from Org. Biomol. Chem.)
Jana Rohacova, Org. Biomol. Chem., 2009, DOI: 10.1039/b912134j
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Source: RSC - Organic Biomolecular Chemistry - September 23, 2009 Category: Molecular Biology Authors: Jana RohacovaM. Luisa Marin Alicia Martinez-Romero Jose-Enrique O'Connor M. Jose Gomez-Lechon M. Teresa Donato Jose V. Castell Miguel A. Miranda Source Type: journals
Evaluation of the pharmacophoric motif of the caged Garcinia xanthonesEmail this article to a colleague.
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Oraphin Chantarasriwong, Woo Cheal Cho, Ayse Batova, Warinthorn Chavasiri, Curtis Moore, Arnold L. Rheingold, Emmanuel A. Theodorakis
(Paper from Org. Biomol. Chem.)
Oraphin Chantarasriwong, Org. Biomol. Chem., 2009, DOI: 10.1039/b913496d
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Source: RSC - Organic Biomolecular Chemistry - September 23, 2009 Category: Molecular Biology Authors: Oraphin ChantarasriwongWoo Cheal Cho Ayse Batova Warinthorn Chavasiri Curtis Moore Arnold L. Rheingold Emmanuel A. Theodorakis Source Type: journals
Structural diversity and differential expression of novel human intersectin 1 isoforms.Email this article to a colleague.
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Intersectin 1 (ITSN1) is an evolutionarily conserved adaptor protein that functions in clathrin-mediated endocytosis, cell signalling and cytoskeleton rearrangements. The ITSN1 gene encodes two main isoforms: a short form (ITSN1-s), which is ubiquitously expressed and consists of two Eps15 homology (EH) domains and five Src homology 3 (SH3) domains, and a long form (ITSN1-l), which is predominantly expressed in the brain and contains three additional domains, a Dbl homology (DH) domain, a Pleckstrin homology (PH) domain and a C2 domain. Using computational analysis of the EST database and 3' RACE we determined the leng...
Source: Molecular Biology Reports - September 23, 2009 Category: Molecular Biology Authors: Kropyvko S, Gerasymchuk D, Skrypkina I, Dergai M, Dergai O, Nikolaienko O, Rynditch A, Tsyba L Tags: Mol Biol Rep Source Type: journals
Molecular analysis of an actin gene, CarACT1, from chickpea (Cicer arietinum L.).Email this article to a colleague.
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In this study, an actin gene was isolated from chickpea for the first time and designated as CarACT1 (for Cicer arietinum L. actin gene 1; Genbank accession no. EU529707). It encoded a putative protein with 377 amino acids and contained five exons and four introns within genomic DNA sequence. CarACT1 was localized in cytoplasm and showed high similarity to other well known actins from various species. Reverse transcription-polymerase chain reaction (RT-PCR) assay proved that CarACT1 transcripts were ubiquitously accumulated in all major organs, such as seedling roots, stems, leaves, flowers, young pods, and seeds, as well ...
Source: Molecular Biology Reports - September 23, 2009 Category: Molecular Biology Authors: Peng H, Cheng H, Yu X, Shi Q, Zhang H, Li J, Ma H Tags: Mol Biol Rep Source Type: journals
Peptide thioester synthesis through N[rightward arrow]S acyl-transfer: application to the synthesis of a [small beta]-defensinEmail this article to a colleague.
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Jaskiranjit Kang, Natalie L. Reynolds, Christine Tyrrell, Julia R. Dorin, Derek Macmillan
(Paper from Org. Biomol. Chem.)
Jaskiranjit Kang, Org. Biomol. Chem., 2009, DOI: 10.1039/b913886b
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Source: RSC - Organic Biomolecular Chemistry - September 23, 2009 Category: Molecular Biology Authors: Jaskiranjit KangNatalie L. Reynolds Christine Tyrrell Julia R. Dorin Derek Macmillan Source Type: journals
Ovarian Cancer Cytoreduction Induces Changes in T Cell Population Subsets Reducing Immunosuppression.Email this article to a colleague.
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In conclusion, we demonstrate in ovarian cancer that primary debulking is associated with a reduction of circulating T(reg) and an increase in CD8 T cell function. Debulking plays a beneficial systemic effect by reverting immunosuppression and restoring immunological fitness.
PMID: 19780872 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - September 23, 2009 Category: Molecular Biology Authors: Napoletano C, Bellati F, Landi R, Pauselli S, Marchetti C, Visconti V, Sale P, Liberati M, Rughetti A, Frati L, Benedetti Panici P, Nuti M Tags: J Cell Mol Med Source Type: journals
Recruitment of endogenous bone marrow mesenchymal stem cells towards injured liver.Email this article to a colleague.
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Abstract Background Recent studies suggest that mesenchymal stem cells (MSCs) possess a greater differentiation potential than once thought and that they have the capacity to regenerate damaged tissues/organs. However, the evidence is insufficient, and the mechanism governing the recruitment and homing of MSCs to these injured sites is not well understood. Methods We first examined the MSCs circulating in peripheral blood and then performed chemotaxis, wound-healing, and tubule-formation assays to investigate the migration capability of mouse bone marrow MSCs (mBM-MSCs) in response to liver-injury signals. In addition,...
Source: J Cell Mol Med - September 23, 2009 Category: Molecular Biology Authors: Chen Y, Xiang LX, Shao JZ, Pan RL, Wang YX, Dong XJ, Zhang GR Tags: J Cell Mol Med Source Type: journals
Enhanced neointimal hyperplasia and carotid artery remodeling in sequestosome 1 deficient mice.Email this article to a colleague.
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Abstract Deficiency in the signal adaptor protein sequestosome 1 (SQSTM1/A170/p62) in mice exhibits mature-onset obesity accompanied by insulin and leptin resistance. We previously established that redox sensitive transcription factor Nrf2 upregulates SQSTM1 expression in response to atherogenic stimuli or laminar shear stress in vascular cells, and here examine role of SQSTM1 in neointimal hyperplasia and vascular remodeling in vivo following carotid artery ligation. Neointimal hyperplasia was markedly enhanced at ligation sites after 3 weeks in SQSTM1(-/-) compared with wild type mice. The intimal area and stenotic r...
Source: J Cell Mol Med - September 23, 2009 Category: Molecular Biology Authors: Sugimoto R, Warabi E, Katayanagi S, Sakai S, Uwayama J, Yanagawa T, Watanabe A, Harada H, Kitamura K, Noguchi N, Yoshida H, Siow RC, Mann GE, Ishii T Tags: J Cell Mol Med Source Type: journals
Dose-dependent effect of radiation on angiogenic and angiostatic CXC chemokine expression in human endothelial cells.Email this article to a colleague.
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Blood vessel growth is regulated by angiogenic and angiostatic CXC chemokines, and radiation is a vasculogenic stimulus. We investigated the effect of radiation on endothelial cell chemokine signaling, receptor expression, and migration and apoptosis. Human umbilical vein endothelial cells were exposed to a single fraction of 0, 5, or 20Gy of ionizing radiation (IR). All vasculogenic chemokines (CXCL1-3/5-8) increased 3-13-fold after 5 or 20Gy IR. 20Gy induced a marked increase (1.6-4-fold) in angiostatic CXC chemokines. CXCR4 expression increased 3.5 and 7-fold at 48h after 5 and 20Gy, respectively. Bone marrow progen...
Source: Cytokine - September 23, 2009 Category: Molecular Biology Authors: Chang CC, Lerman OZ, Thanik VD, Scharf CL, Greives MR, Schneider RJ, Formenti SC, Saadeh PB, Warren SM, Levine JP Tags: Cytokine Source Type: journals
Modulation of Th1/Th2 cytokines and inflammatory mediators by hydroxychavicol in adjuvant induced arthritic tissues.Email this article to a colleague.
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The present study was undertaken to investigate the anti-arthritic activity of hydroxychavicol (HC) a major phenolic compound isolated from the aqueous extract leaves of plant Piper betle (Piperaceae). The compound showed significant lowering of pro-inflammatory (Th1) cytokine levels in arthritic paw tissue homogenate supernatant viz. IL-2, IFN-gamma, and TNF-alpha with maximum inhibition at higher dose levels of 2 and 4mg/kg p.o. and enhanced the production of anti-inflammatory (Th2) cytokines IL-4 and IL-5 estimated by cytometric bead array immunoassay. Cytometric bead array uses the sensitivity of amplified fluoresc...
Source: Cytokine - September 23, 2009 Category: Molecular Biology Authors: Pandey A, Bani S, Dutt P, Suri KA Tags: Cytokine Source Type: journals
Centrosome structure and function under normal and pathological conditionsEmail this article to a colleague.
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No abstract.
Source: Environmental and Molecular Mutagenesis - September 22, 2009 Category: Molecular Biology Authors: Dan L. Sackett, Ofelia Olivero Source Type: journals
RRD1, a component of the TORC1 signalling pathway, affects anaesthetic response in Saccharomyces cerevisiaeEmail this article to a colleague.
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The molecular mechanisms of action of volatile anaesthetics remain unknown despite clinical use for over 150 years. While many effects of these agents have been characterized, clear insight into how these effects relate to the physiological state of anaesthesia has not been established. Volatile anaesthetics arrest cell division in Saccharomyces cerevisiae in a manner that parallels the anaesthetic actions of these drugs in mammals. To gain additional insight into the cellular activities of these drugs, we isolated genes that, when present on multi-copy plasmids, render S. cerevisiae resistant to the volatile anaesthetic i...
Source: Yeast - September 22, 2009 Category: Molecular Biology Authors: Laura K. Palmer, Beverly A. Baptiste, John C. Fester, Justin C. Perkins, Ralph L. Keil Source Type: journals
Phenylenediamine-based bivalent glycocyclophanes: synthesis and analysis of the influence of scaffold rigidity and ligand spacing on lectin binding in cell systems with different glycomic profilesEmail this article to a colleague.
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Sabine Andre, Trinidad Velasco-Torrijos, Rosaria Leyden, Sebastien Gouin, Manuela Tosin, Paul V. Murphy, Hans-Joachim Gabius
(Paper from Org. Biomol. Chem.)
Sabine Andre, Org. Biomol. Chem., 2009, DOI: 10.1039/b913010a
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Source: RSC - Organic Biomolecular Chemistry - September 22, 2009 Category: Molecular Biology Authors: Sabine AndreTrinidad Velasco-Torrijos Rosaria Leyden Sebastien Gouin Manuela Tosin Paul V. Murphy Hans-Joachim Gabius Source Type: journals
Lewis acid InBr3-catalyzed arylation of diorgano diselenides and ditellurides with arylboronic acidsEmail this article to a colleague.
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Kai Ren, Min Wang, Lei Wang
(Paper from Org. Biomol. Chem.)
Kai Ren, Org. Biomol. Chem., 2009, DOI: 10.1039/b914533h
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Source: RSC - Organic Biomolecular Chemistry - September 22, 2009 Category: Molecular Biology Authors: Kai RenMin Wang Lei Wang Source Type: journals
Synthesis of unsaturated phosphatidylinositol 4,5-bisphosphate and analoguesEmail this article to a colleague.
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Nitesh Panchal, Piers R. J. Gaffney
(Paper from Org. Biomol. Chem.)
Nitesh Panchal, Org. Biomol. Chem., 2009, DOI: 10.1039/b907551h
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Source: RSC - Organic Biomolecular Chemistry - September 22, 2009 Category: Molecular Biology Authors: Nitesh PanchalPiers R. J. Gaffney Source Type: journals
Do genetic predictors of pain sensitivity associate with persistent widespread pain?Email this article to a colleague.
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In conclusion, there was no evidence of association between proposed functional SNPs, previously reported to influence pain sensitivity, in GCH1 and OPRM1 with CWP. Further evidence of null association in large independent cohorts is required to truly exclude these SNPs as genetic risk factors for CWP.
Source: Molecular Pain - September 22, 2009 Category: Molecular Biology Authors: Kate HollidayBarbara NichollGary MacfarlaneWendy ThomsonKelly DaviesJohn McBeth Source Type: journals
Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesisEmail this article to a colleague.
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Mitogenic regulation by caveolin-2 in response to insulin was investigated. Insulin triggered phosphorylation of caveolin-2 on tyrosine 19. Insulin increased the interaction between pY19-caveolin-2 and phospho-ERK, and that interaction was inhibited by a MEK inhibitor U0126. Insulin-induced interaction of caveolin-2 with phospho-ERK was prevented when tyrosine 19 is mutated to alanine. Insulin relocalized phospho-ERK and pY19-caveolin-2 to the nucleus and their nuclear co-localization was impaired by U0126. Down-regulation of caveolin-2 by caveolin-2 siRNA arrested the insulin-induced nuclear localization of ERK with no ch...
Source: Journal of Cellular and Molecular Medicine - September 22, 2009 Category: Molecular Biology Authors: Hayeong Kwon, Kyuho Jeong, Yunbae Pak Tags: ARTICLES Source Type: journals
U2OS cells lacking Chk1 undergo aberrant mitosis and fail to activate the spindle checkpointEmail this article to a colleague.
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Chk1 is a conserved protein kinase originally identified in fission yeast, required to delay entry of cells with damaged or unreplicated DNA into mitosis. The requirement of Chk1 for both S and G2/M checkpoints has been elucidated while only few studies have connected Chk1 to the mitotic spindle checkpoint. We used a small interference RNA strategy to investigate the role of Chk1 in unstressed conditions. Chk1 depletion in U2OS human osteosarcoma cells inhibited cell proliferation and raised the percentage of cells with a 4N DNA content, which correlated with accumulation of giant polynucleated cells morphologically distin...
Source: Journal of Cellular and Molecular Medicine - September 22, 2009 Category: Molecular Biology Authors: Laura Carrassa, Yolanda Sanchez, Eugenio Erba, Giovanna Damia Tags: ARTICLES Source Type: journals
Expression and function of CD9 in melanoma cellsEmail this article to a colleague.
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CD9, a member of the tetraspanin family, functions as an organizer in "tetraspanin webs," through interacting with other cell adhesion molecules. It plays a role in differentiation, fertilization, and cell migration. We investigated the expression and function of CD9 in melanoma. CD9 protein expression in B16 mouse melanoma and six human melanoma cell lines was decreased compared to normal melanocytes. B16F1 clones stably overexpressing CD9 had reduced ability to form colonies in soft agar; however, paradoxically these overexpressing clones had increased ability to invade Matrigel. Similarly, transient overexpression of CD...
Source: Molecular Carcinogenesis - September 22, 2009 Category: Molecular Biology Authors: Jun Fan, Guo-Zhang Zhu, Richard M. Niles Source Type: journals
Aberrant DNA methylation occurs in colon neoplasms arising in the azoxymethane colon cancer modelEmail this article to a colleague.
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Mouse models of intestinal tumors have advanced our understanding of the role of gene mutations in colorectal malignancy. However, the utility of these systems for studying the role of epigenetic alterations in intestinal neoplasms remains to be defined. Consequently, we assessed the role of aberrant DNA methylation in the azoxymethane (AOM) rodent model of colon cancer. AOM induced tumors display global DNA hypomethylation, which is similar to human colorectal cancer. We next assessed the methylation status of a panel of candidate genes previously shown to be aberrantly methylated in human cancer or in mouse models of mal...
Source: Molecular Carcinogenesis - September 22, 2009 Category: Molecular Biology Authors: Scott C. Borinstein, Melissa Conerly, Slavomir Dzieciatkowski, Swati Biswas, M. Kay Washington, Patty Trobridge, Steve Henikoff, William M. Grady Source Type: journals
Cold-inducible RNA-binding protein contributes to human antigen R and cyclin E1 deregulation in breast cancerEmail this article to a colleague.
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The cell cycle regulator cyclin E1 is aberrantly expressed in a variety of human cancers. In breast cancer, elevated cyclin E1 correlates with poor outcome, as do high cytoplasmic levels of the stress-induced RNA-binding protein human antigen R (HuR). We showed previously that increased cytoplasmic HuR elevates cyclin E1 in MCF-7 breast cancer cells by stabilizing its mRNA. We show here that cold-inducible RNA-binding protein (CIRP) co-regulates cyclin E1 with HuR in breast cancer cells. CIRP had been shown to interact with HuR in Xenopus laevis oocytes and to be decreased in endometrial cancer. To investigate if human CIR...
Source: Molecular Carcinogenesis - September 22, 2009 Category: Molecular Biology Authors: Xun Guo, Yuehan Wu, Rebecca S. Hartley Source Type: journals
Rapid flow cytometric method for measuring senescence associated [beta]-galactosidase activity in human fibroblastsEmail this article to a colleague.
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Senescence associated-[beta]-galactosidase (SA-[beta]-gal) activity is a widely used marker for cellular senenescence. SA-[beta]-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to better differentiate between populations of fibroblasts in degrees of SA-[beta]-gal activity. Skin fibroblasts were isolated from young (mean age ± SD: 25.5 ± 1.8) and very old (age 90.2 ± 0.3) subjects. Differen...
Source: Cytometry Part A - September 22, 2009 Category: Molecular Biology Authors: Gerard Noppe, Pim Dekker, Corine de Koning-Treurniet, Joke Blom, Diana van Heemst, Roeland W. Dirks, Hans J. Tanke, Rudi G. J. Westendorp, Andrea B. Maier Source Type: journals
