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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 22.

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Synthesis of unsaturated phosphatidylinositol 4,5-bisphosphate and analoguesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nitesh Panchal, Piers R. J. Gaffney (Paper from Org. Biomol. Chem.) Nitesh Panchal, Org. Biomol. Chem., 2009, DOI: 10.1039/b907551h To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - September 22, 2009 Category: Molecular Biology Authors: Nitesh PanchalPiers R. J. Gaffney Source Type: journals

Do genetic predictors of pain sensitivity associate with persistent widespread pain?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, there was no evidence of association between proposed functional SNPs, previously reported to influence pain sensitivity, in GCH1 and OPRM1 with CWP. Further evidence of null association in large independent cohorts is required to truly exclude these SNPs as genetic risk factors for CWP.
Source: Molecular Pain - September 22, 2009 Category: Molecular Biology Authors: Kate HollidayBarbara NichollGary MacfarlaneWendy ThomsonKelly DaviesJohn McBeth Source Type: journals

Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mitogenic regulation by caveolin-2 in response to insulin was investigated. Insulin triggered phosphorylation of caveolin-2 on tyrosine 19. Insulin increased the interaction between pY19-caveolin-2 and phospho-ERK, and that interaction was inhibited by a MEK inhibitor U0126. Insulin-induced interaction of caveolin-2 with phospho-ERK was prevented when tyrosine 19 is mutated to alanine. Insulin relocalized phospho-ERK and pY19-caveolin-2 to the nucleus and their nuclear co-localization was impaired by U0126. Down-regulation of caveolin-2 by caveolin-2 siRNA arrested the insulin-induced nuclear localization of ERK with no ch...
Source: Journal of Cellular and Molecular Medicine - September 22, 2009 Category: Molecular Biology Authors: Hayeong Kwon, Kyuho Jeong, Yunbae Pak Tags: ARTICLES Source Type: journals

U2OS cells lacking Chk1 undergo aberrant mitosis and fail to activate the spindle checkpointEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chk1 is a conserved protein kinase originally identified in fission yeast, required to delay entry of cells with damaged or unreplicated DNA into mitosis. The requirement of Chk1 for both S and G2/M checkpoints has been elucidated while only few studies have connected Chk1 to the mitotic spindle checkpoint. We used a small interference RNA strategy to investigate the role of Chk1 in unstressed conditions. Chk1 depletion in U2OS human osteosarcoma cells inhibited cell proliferation and raised the percentage of cells with a 4N DNA content, which correlated with accumulation of giant polynucleated cells morphologically distin...
Source: Journal of Cellular and Molecular Medicine - September 22, 2009 Category: Molecular Biology Authors: Laura Carrassa, Yolanda Sanchez, Eugenio Erba, Giovanna Damia Tags: ARTICLES Source Type: journals

Expression and function of CD9 in melanoma cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CD9, a member of the tetraspanin family, functions as an organizer in "tetraspanin webs," through interacting with other cell adhesion molecules. It plays a role in differentiation, fertilization, and cell migration. We investigated the expression and function of CD9 in melanoma. CD9 protein expression in B16 mouse melanoma and six human melanoma cell lines was decreased compared to normal melanocytes. B16F1 clones stably overexpressing CD9 had reduced ability to form colonies in soft agar; however, paradoxically these overexpressing clones had increased ability to invade Matrigel. Similarly, transient overexpression of CD...
Source: Molecular Carcinogenesis - September 22, 2009 Category: Molecular Biology Authors: Jun Fan, Guo-Zhang Zhu, Richard M. Niles Source Type: journals

Aberrant DNA methylation occurs in colon neoplasms arising in the azoxymethane colon cancer modelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mouse models of intestinal tumors have advanced our understanding of the role of gene mutations in colorectal malignancy. However, the utility of these systems for studying the role of epigenetic alterations in intestinal neoplasms remains to be defined. Consequently, we assessed the role of aberrant DNA methylation in the azoxymethane (AOM) rodent model of colon cancer. AOM induced tumors display global DNA hypomethylation, which is similar to human colorectal cancer. We next assessed the methylation status of a panel of candidate genes previously shown to be aberrantly methylated in human cancer or in mouse models of mal...
Source: Molecular Carcinogenesis - September 22, 2009 Category: Molecular Biology Authors: Scott C. Borinstein, Melissa Conerly, Slavomir Dzieciatkowski, Swati Biswas, M. Kay Washington, Patty Trobridge, Steve Henikoff, William M. Grady Source Type: journals

Cold-inducible RNA-binding protein contributes to human antigen R and cyclin E1 deregulation in breast cancerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The cell cycle regulator cyclin E1 is aberrantly expressed in a variety of human cancers. In breast cancer, elevated cyclin E1 correlates with poor outcome, as do high cytoplasmic levels of the stress-induced RNA-binding protein human antigen R (HuR). We showed previously that increased cytoplasmic HuR elevates cyclin E1 in MCF-7 breast cancer cells by stabilizing its mRNA. We show here that cold-inducible RNA-binding protein (CIRP) co-regulates cyclin E1 with HuR in breast cancer cells. CIRP had been shown to interact with HuR in Xenopus laevis oocytes and to be decreased in endometrial cancer. To investigate if human CIR...
Source: Molecular Carcinogenesis - September 22, 2009 Category: Molecular Biology Authors: Xun Guo, Yuehan Wu, Rebecca S. Hartley Source Type: journals

Rapid flow cytometric method for measuring senescence associated [beta]-galactosidase activity in human fibroblastsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Senescence associated-[beta]-galactosidase (SA-[beta]-gal) activity is a widely used marker for cellular senenescence. SA-[beta]-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to better differentiate between populations of fibroblasts in degrees of SA-[beta]-gal activity. Skin fibroblasts were isolated from young (mean age ± SD: 25.5 ± 1.8) and very old (age 90.2 ± 0.3) subjects. Differen...
Source: Cytometry Part A - September 22, 2009 Category: Molecular Biology Authors: Gerard Noppe, Pim Dekker, Corine de Koning-Treurniet, Joke Blom, Diana van Heemst, Roeland W. Dirks, Hans J. Tanke, Rudi G. J. Westendorp, Andrea B. Maier Source Type: journals

Flow cytometry and the stability of phycoerythrin-tandem dye conjugatesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Routine clinical flow cytometric procedures demand rigorous, simple, and reproducible procedures for spectral compensation. The current, often laborious, spectral compensation procedures are the result of variability in instrument settings, instrument performance, and variability in reagents. In particular, the use of tandem dye conjugates necessitates elaborate spectral compensation procedures that need to be applied frequently. Manufacturer, lot number, and handling procedures are considered the key aspects affecting the fluorescence characteristics of tandem dyes. A better understanding of how specific conditions affect...
Source: Cytometry Part A - September 22, 2009 Category: Molecular Biology Authors: Ruud Hulspas, David Dombkowski, Frederic Preffer, Derick Douglas, Brian Kildew-Shah, John Gilbert Source Type: journals

Identification of five candidate lung cancer biomarkers by proteomic analysis of conditioned media of four lung cancer cell lines.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Detection of lung cancer at an early stage is necessary for successful therapy and improved survival rates. We performed a bottom-up proteomic analysis employing a two-dimensional LC-MS/MS strategy on the conditioned media (CM) of 4 lung cancer cell lines of different histological backgrounds [non-small cell lung cancer: H23 (adenocarcinoma), H520 (squamous cell carcinoma), H460 (large cell carcinoma) and small cell lung cancer (H1688)] to identify secreted or membrane-bound proteins that could be useful as novel lung cancer biomarkers. Proteomic analysis of the four CM allowed identification of 1,830 different protein...
Source: Molecular and Cellular Proteomics : MCP - September 22, 2009 Category: Molecular Biology Authors: Planque C, Kulasingan V, Smith CR, Reckamp K, Goodglick L, Diamandis EP Tags: Mol Cell Proteomics Source Type: journals

A single-nucleotide polymorphism of the TNFAIP3 gene is associated with systemic lupus erythematosus in Chinese Han population.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Systemic lupus erythematosus (SLE) is a complex systemic disease influenced by genetic and environmental factors. The exact pathogenesis of SLE is still unknown. Recently, several genome-wide association studies (GWA) in European population have found many novel susceptibility genes for SLE including TNFAIP3. In order to examine whether TNFAIP3 is associated with SLE in Chinese Han population, we genotyped one of its non-synonymous mutation SNP rs2230926, showing significant association evidence with SLE in European population, with 1,420 cases and 4,461 controls of Chinese Han by using Sequenom MassArray system. Highl...
Source: Molecular Biology Reports - September 22, 2009 Category: Molecular Biology Authors: Cai LQ, Wang ZX, Lu WS, Han JW, Sun LD, Du WH, Zhang SM, Zuo XB, Zhang XJ, Yang S Tags: Mol Biol Rep Source Type: journals

Isolation and expression analysis of tuberous root development related genes in Rehmannia glutinosa.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we focused on the molecular profiling of the tuberous root development of Rehmannia glutinosa. Suppression subtractive hybridization technology was employed to compare gene expression between adventitious root and developing tuberous root. As a result, a tuberous root subtractive library was constructed and 199 development-related unique expressed sequence tags were identified, which represent different groups of genes involved in metabolism, protein synthesis, protein fate, cell fate, signaling, transcription and development, etc. In order to further validate the obtained result, 18 genes were selected for ...
Source: Molecular Biology Reports - September 22, 2009 Category: Molecular Biology Authors: Sun P, Guo Y, Qi J, Zhou L, Li X Tags: Mol Biol Rep Source Type: journals

Tissue-specific expression of the chicken adipose differentiation-related protein (ADP) gene.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Adipose differentiation-related protein gene (ADP) plays an important role in controlling lipid accumulation in mammals. It may also affect lipid deposition in birds. However, the molecular mechanism of its actions in birds remains unknown. In the present study, the coding sequence of ADP cDNA for Chinese native breed Sichuan Mountainous Black-bone chicken (MB) was first cloned from abdominal fat using reverse transcription-PCR (RT-PCR). This putative MB ADP cDNA (1,881 bp) encodes an open reading frame of 438 amino acids (AA) and shares high AA sequence identity with that of red jungle fowl (99%), duck (92%), house mo...
Source: Molecular Biology Reports - September 22, 2009 Category: Molecular Biology Authors: Zhao X, Zhu Q, Wang Y, Yang Z, Liu Y Tags: Mol Biol Rep Source Type: journals

Modified low density lipoproteins binding requires a lysine cluster region in the murine macrophage scavenger receptor class A type II.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study evaluated the role of the murine SRA-II and a lysine mutated SRA-II on AcLDL uptake. Fluorescence labeled AcLDL uptake was quantified using a Laser Scan Cytometer. A significant increase in fluorescence uptake was found in the cells transfected with SRA-II versus those with empty vector. Cells expressing the lysine mutated SRA-II also demonstrated a significant decrease in their uptake of AcLDL. This data supports the concept that the conserved lysine cluster in murine SRA-II is the binding region for AcLDL or contributes to the trimeric structure of SRA-II necessary for AcLDL binding. PMID: 19774489 [PubMed...
Source: Molecular Biology Reports - September 22, 2009 Category: Molecular Biology Authors: Leyva FJ, Pershouse MA, Holian A Tags: Mol Biol Rep Source Type: journals

Polymorphism of chicken myocyte-specific enhancer-binding factor 2A gene and its association with chicken carcass traits.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Myocyte-specific enhancer-binding factor 2A (MEF2A) gene is a member of the myocyte-specific enhancer-binding factor 2 (MEF2) protein family which involved in vertebrate skeletal muscle development and differentiation. The aim of the current study is to investigate the potential associations between MEF2A gene SNPs (single nucleotide polymorphisms) and the carcass traits in 471 chicken samples from four populations. Three new SNPs (T46023C, A72626G, and T89232G) were detected in the chicken MEF2A gene. The T46023C genotypes were associated with live body weight (BW), carcass weight (CW), eviscerated weight, semi-evisce...
Source: Molecular Biology Reports - September 22, 2009 Category: Molecular Biology Authors: Zhou Y, Liu Y, Jiang X, Du H, Li X, Zhu Q Tags: Mol Biol Rep Source Type: journals

Hec1 Contributes to Mitotic Centrosomal Microtubule Growth for Proper Spindle Assembly through Interaction with Hice1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: Yixian Zheng Previous studies have stipulated Hec1 as a conserved kinetochore component critical for mitotic control in part by directly binding to kinetochore fibers of the mitotic spindle and by recruiting spindle assembly checkpoint proteins Mad1 and Mad2. Hec1 has also been reported to localize to centrosomes, but its function there has yet to be elucidated. Herein we show that Hec1 specifically colocalizes with Hice1, a previously characterized centrosomal microtubule-binding protein, at the spindle pole region during mitosis. In addition, the C-terminal region of Hec1 directly binds to the Coil...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Wu G, Wei R, Cheng E, Ngo B, Lee WH Tags: Mol Biol Cell Source Type: journals

Trafficking of Crumbs3 during Cytokinesis Is Crucial for Lumen Formation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: David G. Drubin Although lumen generation has been extensively studied through so-called cyst-formation assays in Madin-Darby Canine Kidney (MDCK) cells, an underlying mechanism that leads to the initial appearance of a solitary lumen remains elusive. Lumen formation is thought to take place at early stages in aggregates containing only a few cells. Evolutionarily conserved polarity protein complexes, namely the Crumbs, Par, and Scribble complexes, establish apicobasal polarity in epithelial cells, and interference with their function impairs the regulated formation of solitary epithelial lumina. Her...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Schlüter MA, Pfarr CS, Pieczynski J, Whiteman EL, Hurd TW, Fan S, Liu CJ, Margolis B Tags: Mol Biol Cell Source Type: journals

Yeast Sec1p Functions Before and After Vesicle Docking.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: Patrick J. Brennwald SM (Sec1/Munc18) proteins bind cognate SNARE complexes and stimulate vesicle membrane fusion. Before fusion, vesicles are docked to specific target membranes. Regulation of vesicle docking is attributed to some but not all SM proteins, suggesting specialization of this earlier function. Yeast Sec1p appears to function only after vesicles are docked and SNARE complexes are assembled. Here we show that yeast Sec1p is required before and after SNARE complex assembly, in support of general requirements for SM proteins in both vesicle docking and fusion. Two classes of sec1 mutants we...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Hashizume K, Cheng YS, Hutton JL, Chiu CH, Carr CM Tags: Mol Biol Cell Source Type: journals

G{alpha}12 Inhibits {alpha}2{beta}1 Integrin-Mediated MDCK Cell Attachment and Migration on Collagen-I and Blocks Tubulogenesis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: Asma Nusrat Regulation of epithelial cell attachment and migration are essential for normal development and maintenance of numerous tissues. G proteins and integrins are critical signaling proteins regulating these processes, yet in polarized cells little is known about the interaction of these pathways. Herein, we demonstrate that Galpha12 inhibits interaction of MDCK cells with collagen-I, the major ligand for alpha2beta1 integrin. Activating Galpha12 (QL point mutation or stimulating endogenous Galpha12 with thrombin) inhibited focal adhesions and lamellipodia formation and led to impaired cell mi...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Kong T, Xu D, Yu W, Takakura A, Boucher I, Tran M, Kreidberg J, Shah J, Zhou J, Denker BM Tags: Mol Biol Cell Source Type: journals

The Vesicle Inducing Protein 1 from Synechocystis sp. PCC 6803 Organizes into Diverse Higher Ordered Ring Structures.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: Reid Gilmore The "vesicle inducing protein in plastids 1" (Vipp1) was found to be involved in thylakoid membrane formation in chloroplasts and cyanobacteria. In contrast to chloroplasts, it has been suggested that in cyanobacteria the protein is only tightly associated with the cytoplasmic membrane. In the present study we analyze and describe the subcellular localization and the oligomeric organization of Vipp1 from the cyanobacterium Synechocystis PCC 6803. Vipp1 forms stable dimers and higher ordered oligomers in the cytoplasm as well as at both the cytoplasmic and thylakoid membrane. Vipp1 oligom...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Fuhrmann E, Bultema JB, Kahmann U, Rupprecht E, Boekema EJ, Schneider D Tags: Mol Biol Cell Source Type: journals

Dkk-1 Inhibits Intestinal Epithelial Cell Migration by Attenuating Directional Polarization of Leading Edge Cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report, we show that Dkk-1 is secreted by intestinal epithelial cells after wounding, and inhibits cell migration by attenuating the directional orientation of migrating epithelial cells. Dkk-1 exposure induced mislocalized activation of Cdc42 in migrating cells, which coincided with a displacement of the polarity protein Par6 from the leading edge. Consequently, the relocation of the microtubule organizing center and the Golgi apparatus in the direction of migration was significantly and persistently inhibited in the presence of Dkk-1. siRNA-induced down-regulation of Dkk-1 confirmed that extracellular exposure to...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Koch S, Capaldo CT, Samarin S, Nava P, Neumaier I, Skerra A, Sacks DB, Parkos CA, Nusrat A Tags: Mol Biol Cell Source Type: journals

Early-Arriving Syp1p and Ede1p Function in Endocytic Site Placement and Formation in Budding Yeast.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: Sandra L. Schmid Recent studies have revealed the detailed timing of protein recruitment to endocytic sites in budding yeast. However, little is understood about the early stages of their formation. Here we identify the septin-associated protein Syp1p as a component of the machinery that drives clathrin-mediated endocytosis in budding yeast. Syp1p arrives at endocytic sites early in their formation, and shares unique dynamics with the EH-domain protein, Ede1p. We find that Syp1p is related in amino acid sequence to several mammalian proteins one of which, SGIP1-alpha, is an endocytic component that b...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Stimpson HE, Toret CP, Cheng AT, Pauly BS, Drubin DG Tags: Mol Biol Cell Source Type: journals

Direct and Indirect Roles of Cdk5 as an Upstream Regulator in the JNK Cascade: Relevance to Neurotoxic Insults in Alzheimer's Disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study presents the first mechanism showing Cdk5 as a major regulator of JNK cascade. Deregulated Cdk5 induces biphasic activation of JNK pathway. The first phase revealed c-Jun as a direct substrate of Cdk5, whose activation is independent of reactive oxygen species (ROS) and JNK. In the second phase, Cdk5 activates c-Jun via ROS-mediated activation of JNK. Rapid c-Jun activation is supported by in vivo data showing c-Jun phosphorylation in cerebral cortex upon p25 induction in Tg mice. Cdk5-mediated biphasic activation of c-Jun highlights c-Jun, rather than JNK, as an important therapeutic target, which was confirmed...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Sun KH, Lee HG, Smith MA, Shah K Tags: Mol Biol Cell Source Type: journals

Spatiotemporal Regulation of Chloride Intracellular Channel protein CLIC4 by RhoA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: J. Silvio Gutkind CLIC4 (Chloride Intracellular Channel 4) is a soluble protein structurally related to omega-type glutathione-S-transferases (GSTs) and implicated in various biological processes, ranging from chloride channel formation to vascular tubulogenesis. However, its function(s) and regulation remain unclear. Here we show that cytosolic CLIC4 undergoes rapid but transient translocation to discrete domains at the plasma membrane upon stimulation of G13-coupled, RhoA-activating receptors, such as those for lysophosphatidic acid, thrombin and sphingosine-1-phosphate. CLIC4 recruitment is strict...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Ponsioen B, van Zeijl L, Langeslag M, Berryman M, Littler D, Jalink K, Moolenaar WH Tags: Mol Biol Cell Source Type: journals

Paxillin-Kinase-Linker Tyrosine Phosphorylation Regulates Directional Cell Migration.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: Josephine C. Adams Directed cell migration requires the coordination of growth factor and cell adhesion signaling and is of fundamental importance during embryonic development, wound repair and pathological conditions such as tumor metastasis. Herein, we demonstrate that the ArfGAP, paxillin-kinase-linker (PKL/GIT2) is tyrosine phosphorylated in response to platelet-derived growth factor (PDGF) stimulation, in an adhesion dependent manner and is necessary for directed cell migration. Using a combination of pharmacological inhibitors, knockout cells and kinase mutants, FAK and Src family kinases were ...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Yu JA, Deakin NO, Turner CE Tags: Mol Biol Cell Source Type: journals

Membrane Insertion of the Pleckstrin Homology Domain Variable Loop 1 Is Critical for Dynamin-catalyzed Vesicle Scission.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: David G. Drubin The GTPase dynamin catalyzes the scission of deeply invaginated clathrin-coated pits at the plasma membrane, but the mechanisms governing dynamin-mediated membrane fission remain poorly understood. Through mutagenesis, we have altered the hydrophobic nature of the membrane-inserting variable loop 1 (VL1) of the pleckstrin homology (PH) domain of dynamin 1 and demonstrate that its stable insertion into the lipid bilayer is critical for high membrane curvature generation and subsequent membrane fission. Dynamin PH domain mutants defective in curvature generation regain function when ass...
Source: Mol Biol Cell - September 22, 2009 Category: Molecular Biology Authors: Ramachandran R, Pucadyil TJ, Liu YW, Acharya S, Leonard M, Lukiyanchuk V, Schmid SL Tags: Mol Biol Cell Source Type: journals

Calcineurin A-beta is required for hypertrophy but not matrix expansion in the diabetic kidney.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, loss of the beta isoform of calcineurin is sufficient to reproduce beneficial aspects of cyclosporine on diabetic renal hypertrophy but not matrix expansion. Therefore, while multiple signals appear to regulate matrix, calcineurin beta appears to be a central mechanism involved in organ hypertrophy. PMID: 19778355 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - September 22, 2009 Category: Molecular Biology Authors: Reddy RN, Knotts TL, Roberts BR, Molkentin JD, Price SR, Gooch JL Tags: J Cell Mol Med Source Type: journals

Hemoglobin, nitric oxide and molecular mechanisms of hypoxic vasodilation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The protected transport of nitric oxide (NO) by hemoglobin (Hb) links the metabolic activity of working tissue to the regulation of its local blood supply through hypoxic vasodilation. This physiologic mechanism is allosterically coupled to the O(2) saturation of Hb and involves the covalent binding of NO to a cysteine residue in the beta-chain of Hb (Cys beta93) to form S-nitrosohemoglobin (SNO-Hb). Subsequent S-transnitrosation, the transfer of NO groups to thiols on the RBC membrane and then in the plasma, preserves NO vasodilator activity for delivery to the vascular endothelium. This SNO-Hb paradigm provides insig...
Source: Trends in Molecular Medicine - September 22, 2009 Category: Molecular Biology Authors: Allen BW, Stamler JS, Piantadosi CA Tags: Trends Mol Med Source Type: journals

The unsung reviewerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nature Structural & Molecular Biology 16, 899 (2009). doi:10.1038/nsmb0909-899 Manuscript peer reviewing is at the heart of the scientific system, but it seems that these duties are often not properly (if at all) recognized by universities, funding agencies or even the rest of the scientific community.
Source: Nature Structural and Molecular Biology - September 22, 2009 Category: Molecular Biology Tags: Editorial Source Type: journals

The p53 transcriptional synapse: activation on demandEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
eppert The adequate and on-time response of cellular transcription to internal needs and external stimuli is a delicate task. Is the transcriptional regulator p53 on call to permit rapid activation of its target genes? A new study reports interplay of p53 with a previously unidentified regulator, calcineurin-binding protein 1 (Cabin1), and provides evidence for such a mechanism.
Source: Nature Structural and Molecular Biology - September 22, 2009 Category: Molecular Biology Authors: Genrich V TolstonogWolfgang Deppert Tags: News and Views Source Type: journals

When chromatin meets splicingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
J Blencowe Using bioinformatics analysis of previously published global genome deep-sequencing data, two papers now show that DNA sequences associated with nucleosomes are preferentially located in exons. The correlation between nucleosome distribution and the exon-intron organization of genes may have a key role in exon recognition at the pre-mRNA level during co-transcriptional splicing, consistent with previous findings indicating chromatin-mediated regulation of alternative splicing.
Source: Nature Structural and Molecular Biology - September 22, 2009 Category: Molecular Biology Authors: Alberto R KornblihttIgnacio E SchorMariano AlloBenjamin J Blencowe Tags: News and Views Source Type: journals

New functions for an ancient domainEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nature Structural & Molecular Biology 16, 904 (2009). doi:10.1038/nsmb0909-904 Author: W Lee Kraus Macrodomains function as binding modules for metabolites of NAD+, including poly(ADP-ribose). Three new studies explore how binding of poly(ADP-ribose) by the macrodomains of histone variant macroH2A1.1 and the ATP-dependent chromatin-remodeling protein ALC1 (also called CHD1L) leads to the modulation of chromatin structure, regulating nuclear functions such as DNA-damage detection and repair.
Source: Nature Structural and Molecular Biology - September 22, 2009 Category: Molecular Biology Authors: W Lee Kraus Tags: News and Views Source Type: journals

Dangerous play—splitting the message may leave you empty handedEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dangerous play—splitting the message may leave you empty handed Nature Structural & Molecular Biology 16, 907 (2009). doi:10.1038/nsmb0909-907 Authors: Yvonne Klaue & Klemens J Hertel An important aspect of eukaryotic gene expression is the efficient integration of transcription, pre-mRNA processing and nuclear export. A new study demonstrates that pre-mRNA transcript continuity is an essential component for maintaining productive coupling of transcription and RNA processing events.
Source: Nature Structural and Molecular Biology - September 22, 2009 Category: Molecular Biology Authors: Yvonne KlaueKlemens J Hertel Tags: News and Views Source Type: journals

Research highlightsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nature Structural & Molecular Biology 16, 909 (2009). doi:10.1038/nsmb0909-909
Source: Nature Structural and Molecular Biology - September 22, 2009 Category: Molecular Biology Tags: Research Highlights Source Type: journals

Differential modulation of mitochondrial OXPHOS system during HIV-1 induced T-cell apoptosis: up regulation of Complex-IV subunit COX-II and its possible implicationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Human Immunodeficiency Virus-1 (HIV-1) infection leads to CD4+ T cell depletion primarily by apoptosis employing both intrinsic and extrinsic pathways. Although extensive literature exists about the role of mitochondrial proteins in HIV induced T cell apoptosis, there is little understanding about the role of different components of mitochondrial oxidative phosphorylation (OXPHOS) system in apoptosis. The OXPHOS system comprises of five enzyme complexes (Complex I, II, III, IV, V), subunits of which have been implicated in various functions in addition to their primary role in energy generating proc...
Source: Apoptosis - September 22, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals

Multiple alphaII-spectrin breakdown products distinguish calpain and caspase dominated necrotic and apoptotic cell death pathwaysEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, three chemical agents, maitotoxin (MTX), staurosporine (STS) and thylenediaminetetraacetic acid (EDTA), were used to induce different types of cell death in PC12 neuronal-like cells. MTX-induced necrosis, as determined by the increased levels of calpain-specific cleaved fragments of spectrin by antibodies specific to the calpain-cleaved 150 kDa αII-spectrin breakdown product (SBDP150) and 145 kDa αII-spectrin breakdown product (SBDP145). In this paradigm, there were no detectable SBDP150i and SBDP120 fragments as determined by antibodies specific to the caspase-cleaved specific fragments sim...
Source: Apoptosis - September 22, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals

Endoplasmic reticulum stress mediates γ-tocotrienol-induced apoptosis in mammary tumor cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract γ-Tocotrienol, a member of the vitamin E family of compounds, induces apoptosis in a variety of cancer cell types. However, previous studies have clearly demonstrated that γ-tocotrienol-induced apoptosis in neoplastic mouse +SA mammary epithelial cells is not mediated through mitochondrial stress or death receptor apoptotic signaling. Therefore, studies were conducted to determine the role of endoplasmic reticulum (ER) stress in mediating γ-tocotrienol-induced apoptosis in +SA mammary tumor cells. Treatment with 15–40 μM γ-tocotrienol induced +SA cell death in a dose-responsive manner, a...
Source: Apoptosis - September 22, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals

Amino acid transport in thermophiles: characterization of an arginine-binding protein in Thermotoga maritimaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Matthew S. Luchansky, Bryan S. Der, Sabato D'Auria, Gabriella Pocsfalvi, Luisa Iozzino, Daniela Marasco, Jonathan D. Dattelbaum (From Mol. BioSyst.) Matthew S. Luchansky, Mol. BioSyst., 2009, DOI: 10.1039/b908412f To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chemical Biology Virtual Journal latest articles - September 21, 2009 Category: Molecular Biology Authors: Matthew S. LuchanskyBryan S. Der Sabato D'Auria Gabriella Pocsfalvi Luisa Iozzino Daniela Marasco Jonathan D. Dattelbaum Source Type: journals

Molecular recognition of poly(A) targeting by protoberberine alkaloids: in vitro biophysical studies and biological perspectivesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Prabal Giri, Gopinatha Suresh Kumar (Review from Mol. BioSyst.) Prabal Giri, Mol. BioSyst., 2009, DOI: 10.1039/b910706a To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 21, 2009 Category: Molecular Biology Authors: Prabal GiriGopinatha Suresh Kumar Source Type: journals

A residue outside the active site CXXC motif regulates the catalytic efficiency of Glutaredoxin 3Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Talia Shekhter, Norman Metanis, Philip E. Dawson, Ehud Keinan (Paper from Mol. BioSyst.) Talia Shekhter, Mol. BioSyst., 2009, DOI: 10.1039/b912753d To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 21, 2009 Category: Molecular Biology Authors: Talia ShekhterNorman Metanis Philip E. Dawson Ehud Keinan Source Type: journals

Direct metallation of thienopyrimidines using a mixed lithium-cadmium base and antitumor activity of functionalized derivativesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Katia Snegaroff, Frederic Lassagne, Ghenia Bentabed-Ababsa, Ekhlass Nassar, Sidaty Cheikh Sid Ely, Stephanie Hesse, Enrico Perspicace, Aicha Derdour, Florence Mongin (Paper from Org. Biomol. Chem.) Katia Snegaroff, Org. Biomol. Chem., 2009, DOI: 10.1039/b915274a To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - September 21, 2009 Category: Molecular Biology Authors: Katia SnegaroffFrederic Lassagne Ghenia Bentabed-Ababsa Ekhlass Nassar Sidaty Cheikh Sid Ely Stephanie Hesse Enrico Perspicace Aicha Derdour Florence Mongin Source Type: journals

Intra-individual polymorphism in diploid and apomictic polyploid hawkweeds (Hieracium, Lactuceae, Asteraceae): disentangling phylogenetic signal, reticulation, and noiseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The high level of intra-individual ETS polymorphism prevented straightforward phylogenetic analysis. Characterization of this variation as additive, shared informative, homoplasious, or unique made it possible to uncover the phylogenetic signal and to reveal the hybrid origin of 29 out of 60 accessions. Contrary to expectation, diploid sexuals and polyploid apomicts did not differ in their molecular patterns. The basic division of the genus into two major clades had not previously been intimated on morphological grounds. Both major groups are thought to have survived in different glacial refugia and to have hy...
Source: BMC Evolutionary Biology - Latest articles - September 21, 2009 Category: Molecular Biology Authors: Judith FehrerKarol KrakJindrich Chrtek Source Type: journals

From sequence to dynamics: the effects of transcription factor and polymerase concentration changes on activated and repressed promotersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The number of regulatory sites may thus act as a versatility-producing device, in addition to serving as a source of robustness of the transcription machinery. Furthermore, our results show that the effects of TF concentration fluctuations on promoter occupancy are constrained by RNAP concentrations.
Source: BMC Molecular Biology - Latest articles - September 21, 2009 Category: Molecular Biology Authors: Abel Gonzalez PerezVladimir Espinosa AngaricaJulio Collado-VidesAna Vasconcelos Source Type: journals

Mirrors in the PDB: left-handed alpha-turns guide design with D-amino acidsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: By examining left-handed -turns containing L-amino acids, new interaction motifs for incorporating D-amino acids into right-handed alpha-helices are identified. These will provide a basis for de novo design of novel heterochiral protein folds.
Source: BMC Structural Biology - Latest articles - September 21, 2009 Category: Molecular Biology Authors: Srinivas AnnavarapuVikas Nanda Source Type: journals

Modeling system states in liver cells: Survival, apoptosis and their modifications in response to viral infectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: A network model for apoptosis and crosstalk in hepatocytes shows four different system states and reproduces a number of different conditions around apoptosis including effects of different growth substrates and viral infections. It produces semi-quantitative predictions on the activity of individual nodes, agreeing with experimental data. The model (SBML format) and all data are available for further predictions and development
Source: BMC Systems Biology - Latest articles - September 21, 2009 Category: Molecular Biology Authors: Nicole PhilippiDorothee WalterRebekka SchlatterKarine FerreiraMichael EdererOliver SawodnyJens TimmerChristoph BornerThomas Dandekar Source Type: journals

Nociception-induced spatial and temporal plasticity of synaptic connection and function in the hippocampal formation of rats: a multi-electrode array recordingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Peripheral persistent nociception produces great impact upon the higher brain structures that lead to not only temporal plasticity, but also spatial plasticity of synaptic connection and function in the HF. The spatial plasticity of synaptic activities is more complex than the temporal plasticity, comprising of enlargement of synaptic connection size at network level, deformed fEPSP at local circuit level and, increased synaptic efficacy at cellular level. In addition, the multi-synaptic model established in the present investigation may open a new avenue for future studies of pain-related brain dysfunctions at...
Source: Molecular Pain - September 21, 2009 Category: Molecular Biology Authors: Xiao-Yan ZhaoMing-Gang LiuDong-Liang YuanYan WangYing HeDan-Dan WangXue-Feng ChenFu-Kang ZhangHua LiXiao-Sheng HeJun Chen Source Type: journals

NMDA receptor subunit expression and PAR2 receptor activation in colospinal afferent neurons (CANs) during inflammation induced visceral hypersensitivityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our data show that inflammation causes an up-regulation of the NMDA receptor and the activation of the PAR2 receptor expressed on CANs. These changes correlate with a decrease in balloon pressure in response to colorectal distention in non-RTX and RTX lesioned animals. Therefore, these data suggest that CANs contribute to visceral hypersensitivity during inflammation.
Source: Molecular Pain - September 21, 2009 Category: Molecular Biology Authors: Shelby SuckowRobert Caudle Source Type: journals

Large A-fiber activity is required for microglial proliferation and p38 MAPK activation in the spinal cord: different effects of resiniferatoxin and bupivacaine on spinal microglial changes after spared nerve injuryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: (1) Blocking peripheral input in TRPV1-positive fibers (presumably C-fibers) is not enough to prevent nerve injury-induced spinal microglia activation. (2) Peripheral input from large myelinated fibers is important for microglial activation. (3) Microglial activation is associated with mechanical allodynia.
Source: Molecular Pain - September 21, 2009 Category: Molecular Biology Authors: Marc SuterTemugin BertaYong-Jing GaoIsabelle DecosterdRu-Rong Ji Source Type: journals

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