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Poem: The Student's Guide to a Perfect Transcript
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: Indira Rajagopal Tags: The Lighter Side Source Type: research
Using the Computer Game “FoldIt” to Entice Students to Explore External Representations of Protein Structure in a Biochemistry Course for Nonmajors
This article describes a novel approach to teaching novice Biochemistry students visual literacy skills and understanding of some aspects of protein structure using the internet resource FoldIt and a worksheet based on selected Introductory Puzzles from this computer game. In responding to a questionnaire, students indicated that they (94%) enjoyed playing the game and furthermore, they indicated that they (100%) perceived an improvement in their understanding of protein structure as a result. Instructor observation of the students using the game together with the worksheet corroborated the results of the student perceptio...
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: Peter C. Farley Tags: Multimedia in Biochemistry and Molecular Biology Education Source Type: research
Problem‐solving Test: Analysis of DNA Damage Recognizing Proteins in Yeast and Human Cells
Terms to be familiar with before you start to solve the test: DNA repair, germline mutation, somatic mutation, inherited disease, cancer, restriction endonuclease, radioactive labeling, [α‐32P]ATP, [γ‐32P]ATP, DNA ligase, polynucleotide kinase, DNA polymerase, terminal transferase, DNA helicase, UV‐irradiation, pyrimidine dimer, base modifications, mismatch, polyacrylamide gel electrophoresis, autoradiography, molecular hybridization, satellite DNA, nucleosomes. © 2012 by The International Union of Biochemistry and Molecular Biology, 41(1):52–54, 2013
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: József Szeberényi Tags: Student Centered Education Source Type: research
Commentary: Prerequisite Knowledge
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: Ann T. S. Taylor Tags: Student Centered Education Source Type: research
Genome annotation in a community college cell biology lab
The Biology Department at Salt Lake Community College has used the IMG‐ACT toolbox to introduce a genome mapping and annotation exercise into the laboratory portion of its Cell Biology course. This project provides students with an authentic inquiry‐based learning experience while introducing them to computational biology and contemporary learning skills. Additionally, the project strengthens student understanding of the scientific method and contributes to student learning gains in curricular objectives centered around basic molecular biology, specifically, the Central Dogma. Importantly, inclusion of this project in ...
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: C. Timothy Beagley Tags: Special Section: Innovative Laboratory Exercises—Focus on Genomic Annotation Source Type: research
Using microbial genome annotation as a foundation for collaborative student research
We used the Integrated Microbial Genomes Annotation Collaboration Toolkit as a framework to incorporate microbial genomics research into a microbiology and biochemistry course in a way that promoted student learning of bioinformatics and research skills and emphasized teamwork and collaboration as evidenced through multiple assessment mechanisms. Student teams in microbiology used bioinformatics tools to identify and characterize gene products from Mucilaginibacter paludis necessary for the synthesis of specific amino acids and then designed and carried out growth experiments to determine if the organism could indeed synth...
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: Kelynne E. Reed, John M. Richardson Tags: Special Section: Innovative Laboratory Exercises—Focus on Genomic Annotation Source Type: research
Strategies for Using Peer‐assisted Learning Effectively in an Undergraduate Bioinformatics Course
This study used a mixed methods approach to evaluate hybrid peer‐assisted learning approaches incorporated into a bioinformatics tutorial for a genome annotation research project. Quantitative and qualitative data were collected from undergraduates who enrolled in a research‐based laboratory course during two different academic terms at UCLA. Findings indicate that a critical feature of the peer‐assisted learning approach is to have near‐peer leaders with genome annotation experience, allowing them to communicate technical and conceptual aspects of the process in the context of a research project (a.k.a., the “bi...
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: Casey Shapiro, Carlos Ayon, Jordan Moberg‐Parker, Marc Levis‐Fitzgerald, Erin R. Sanders Tags: Special Section: Innovative Laboratory Exercises—Focus on Genomic Annotation Source Type: research
Integrating Grant‐funded Research into the Undergraduate Biology Curriculum Using IMG‐ACT
It has become clear in current scientific pedagogy that the emersion of students in the scientific process in terms of designing, implementing, and analyzing experiments is imperative for their education; as such, it has been our goal to model this active learning process in the classroom and laboratory in the context of a genuine scientific question. Toward this objective, the National Science Foundation funded a collaborative research grant between a primarily undergraduate institution and a research‐intensive institution to study the chemotactic responses of the bacterium Pseudomonas putida F1. As part of the project,...
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: Jayna L. Ditty, Kayla M. Williams, Megan M. Keller, Grischa Y. Chen, Xianxian Liu, Rebecca E. Parales Tags: Special Section: Innovative Laboratory Exercises—Focus on Genomic Annotation Source Type: research
Introduction: Sequences and consequences
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: Cheryl A. Kerfeld Tags: Special Section: Innovative Laboratory Exercises–Focus on Genomic Annotation Source Type: research
Improved performance of students instructed in a hybrid PBL format
As a result of enrollment expansion, increasing numbers of students are entering into medical school in China. This combined with a shortage of teachers, means that the learning environment typically consists of a large classroom setting with traditional lecture‐based learning (LBL) as the major mode to teaching and learning. In this article, we investigate the preferences for hybrid problem‐based learning (hybrid‐PBL) or LBL in a large classroom setting. Two hundred five second‐year undergraduate students in Third Military Medical University were randomly divided to two groups. The hybrid‐PBL group contained 101...
Source: Biochemistry and Molecular Biology Education - January 28, 2013 Category: Biochemistry Authors: Jiqin Lian, Fengtian He Tags: Article Source Type: research
Genetic tools to manipulate MRI contrast
Advances in molecular biology in the early 1970s revolutionized research strategies for the study of complex biological processes, which, in turn, created a high demand for new means to visualize these dynamic biological changes noninvasively and in real time. In this respect, MRI was a perfect fit, because of the versatile possibility to alter the different contrast mechanisms. Genetic manipulations are now being translated to MRI through the development of reporters and sensors, as well as the imaging of transgenic and knockout mice. In the past few years, a new molecular biology toolset, namely optogenetics, has emerged...
Source: NMR in Biomedicine - January 28, 2013 Category: Radiology Authors: Raag D. Airan, Nan Li, Assaf A. Gilad, Galit Pelled Tags: Special issue review article Source Type: research
Label-free DNA quantification via a 'pipette, aggregate and blot' (PAB) approach with magnetic silica particles on filter paper
Lab Chip, 2013, Advance ArticleDOI: 10.1039/C2LC40975E, PaperJingyi Li, Qian Liu, Hussein Alsamarri, Jenny A. Lounsbury, Doris M. Haversitick, James P. LandersReliable measurement of DNA concentration is essential for a broad range of applications in biology and molecular biology, and for many of these, quantifying the nucleic acid content is inextricably linked to obtaining optimal results.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Lab Chip latest articles - January 27, 2013 Category: Chemistry Authors: Jingyi Li Source Type: research
Short-term mechanical stress inhibits osteoclastogenesis via suppression of DC-STAMP in RAW264.7 cells.
In this study, we examined the effects of short-term mechanical stress on osteoclastogenesis by applying tensile force to RAW264.7 cells stimulated with receptor activator of nuclear factor-κB ligand (RANKL) using a Flexercell tension system. We counted the number of osteoclasts that were tartrate-resistant acid phosphatase (TRAP)-positive and multinucleated (two or more nuclei) with or without application of mechanical stress for 24 h. Osteoclast number was lower after mechanical stress compared with no mechanical stress. Furthermore, mechanical stress for up to 24 h caused dow...
Source: International Journal of Molecular Medicine - January 27, 2013 Category: Molecular Biology Authors: Kameyama S, Yoshimura Y, Kameyama T, Kikuiri T, Matsuno M, Deyama Y, Suzuki K, Iida J Tags: Int J Mol Med Source Type: research
Synergistic effects of histone deacetylase inhibitor in combination with mTOR inhibitor in the treatment of prostate carcinoma.
Synergistic effects of histone deacetylase inhibitor in combination with mTOR inhibitor in the treatment of prostate carcinoma. Int J Mol Med. 2013 Feb;31(2):339-46 Authors: Thelen P, Krahn L, Bremmer F, Strauss A, Brehm R, Loertzer H Abstract The aim of this study was to elucidate whether the treatment of a prostate carcinoma cell line (LNCaP) and LNCaP-derived tumors with the histone deacetylase (HDAC) inhibitor valproate in combination with the mammalian target of rapamycin (mTOR) inhibitor temsirolimus resulted in synergistic effects on cell proliferation and tumor growth. ...
Source: International Journal of Molecular Medicine - January 27, 2013 Category: Molecular Biology Authors: Thelen P, Krahn L, Bremmer F, Strauss A, Brehm R, Loertzer H Tags: Int J Mol Med Source Type: research
The role of peptide YY in gastrointestinal diseases and disorders (Review).
Abstract Peptide YY (PYY) is affected in several gastrointestinal diseases and disorders. Changes in PYY appear to be an adaptive response to alterations in pathophysiological conditions caused by the disease. This applies to gastrointestinal diseases/disorders such as irritable bowel syndrome, inflammatory bowel disease, celiac disease, systemic sclerosis, and post-intestinal resection. By contrast, the changes in PYY in chronic idiopathic slow transit constipation (CST) seem to be of a primary nature, and may be one etiological factor of the disease. Abnormalities in PYY ...
Source: International Journal of Molecular Medicine - January 27, 2013 Category: Molecular Biology Authors: El-Salhy M, Mazzawi T, Gundersen D, Hatlebakk JG, Hausken T Tags: Int J Mol Med Source Type: research
A novel dual oxidase maturation factor 2 gene mutation for congenital hypothyroidism.
This study is the first to report a novel c.413-414insA (Y138X) mutation for CH, thereby expanding the mutational spectrum of the DUOXA2 gene. PMID: 23292166 [PubMed - in process]
Source: International Journal of Molecular Medicine - January 27, 2013 Category: Molecular Biology Authors: Yi RH, Zhu WB, Yang LY, Lan L, Chen Y, Zhou JF, Wang J, Su YQ Tags: Int J Mol Med Source Type: research
Anti-metastatic effect of polysaccharide isolated from Colocasia esculenta is exerted through immunostimulation.
Anti-metastatic effect of polysaccharide isolated from Colocasia esculenta is exerted through immunostimulation. Int J Mol Med. 2013 Feb;31(2):361-8 Authors: Park HR, Lee HS, Cho SY, Kim YS, Shin KS Abstract In the present study, an edible corm of the plant Colocasia esculenta, commonly known as Taro was extracted with cold water (4˚C). Finally, 10.44 g (1.04%) of the crude polysaccharide (Taro-0) was obtained from Taro. The purified active compound (Taro-4-I) was isolated using DEAE-Sepharose FF and Sephadex G-100. The anti-complementary activity of Taro-4-I (57.3±4.5%)...
Source: International Journal of Molecular Medicine - January 27, 2013 Category: Molecular Biology Authors: Park HR, Lee HS, Cho SY, Kim YS, Shin KS Tags: Int J Mol Med Source Type: research
Microarray-based analysis of the gene expression profile in GC-1 spg cells transfected with spermatogenesis associated gene 12.
Microarray-based analysis of the gene expression profile in GC-1 spg cells transfected with spermatogenesis associated gene 12. Int J Mol Med. 2013 Feb;31(2):459-66 Authors: Lin Y, Liu Z, Liu X, Zhang Y, Rong Z, Li D Abstract The unique differentiation mechanisms of spermatogenesis suggest the existence of cell type- and stage-specific molecules. Herein, a microarray-based approach was used to identify changes in the gene expression profile in mouse GC-1 spg germ cells transfected with spermatogenesis associated gene 12 (SPATA12) . One hundred and eighty-two upregulated genes ...
Source: International Journal of Molecular Medicine - January 27, 2013 Category: Molecular Biology Authors: Lin Y, Liu Z, Liu X, Zhang Y, Rong Z, Li D Tags: Int J Mol Med Source Type: research
Follow-up of secondary diabetic complications after pancreas transplantation.
Abstract PURPOSE OF REVIEW: Successful pancreas transplantation restores physiologic glycemic and metabolic control. Its effects on overall patient survival (especially for simultaneous pancreas-kidney transplantation) are clear-cut. We herein review the available literature to define the impact of pancreas transplantation on chronic complications of diabetes mellitus. RECENT FINDINGS: With longer-term follow-up, wider patient populations, and more accurate investigational tools (clinical and functional tests, noninvasive imaging, histology, and molecular biology), growing data show that successful pancreas tra...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Boggi U, Rosati CM, Marchetti P Tags: Curr Opin Organ Transplant Source Type: research
Diffusion Tensor Imaging of White Matter Tracts in the Dog Brain
Abstract Diffusion weighted imaging sequences are now widely available on Magnetic Resonance Imaging (MRI) scanners. Diffusion Tensor Imaging (DTI) of the brain is able to show white matter tracts and is now commonly used in human medicine to study brain anatomy, tumors, structural pathways,… The purpose of this study was to show the interest of DTI to reveal the white matter fibers in the dogs' brain. DTI MR Images for this study were obtained with a 3 T system of 4 dogs euthanized for other reasons than neurological disorders. Combined fractional anisotropic (FA) and directional maps were obtained in the first 2 hours ...
Source: The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology - January 27, 2013 Category: Molecular Biology Authors: Olivier Jacqmot, Bert Van Thielen, Yves Fierens, Martha Hammond, Inneke Willekens, Peter Van Schuerbeek, Filip Verhelle, Peter Goossens, Filip De Ridder, Jan Pieter Clarys, Anne Vanbinst, Johan De Mey Tags: Neuroscience Source Type: research
Lumbar Vertebral Body Bone Microstructural Scaling in Small to Medium‐Sized Strepsirhines
Abstract Bone mass, architecture, and tissue mineral density contribute to bone strength. As body mass (BM) increases any one or combination of these properties could change to maintain structural integrity. To better understand the structural origins of vertebral fragility and gain insight into the mechanisms that govern bone adaptation, we conducted an integrative analysis of bone mass and microarchitecture in the last lumbar vertebral body from nine strepsirhine species, ranging in size from 42 g (Microcebus rufus) to 2,440 g (Eulemur macaco). Bone mass and architecture were assessed via µCT for the whole body and sphe...
Source: The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology - January 27, 2013 Category: Molecular Biology Authors: Roberto J. Fajardo, Jeremy M. Desilva, Rajaram K. Manoharan, James E. Schmitz, Laura M. Maclatchy, Mary L. Bouxsein Tags: Bone Biology Source Type: research
Types of Imaging, Part 3: Atomic Force Microscopy
Source: The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology - January 27, 2013 Category: Molecular Biology Authors: Ellen Jensen Tags: Cover Image Source Type: research
Mol Biol Cell; +121 new citations
121 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Mol Biol Cell These pubmed results were generated on 2013/01/26PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources.
Source: Mol Biol Cell - January 26, 2013 Category: Molecular Biology Tags: Report Source Type: research
Bench‐top validation testing of selected immunological and molecular Renibacterium salmoninarum diagnostic assays by comparison with quantitative bacteriological culture
Abstract No gold standard assay exhibiting error‐free classification of results has been identified for detection of Renibacterium salmoninarum, the causative agent of salmonid bacterial kidney disease. Validation of diagnostic assays for R. salmoninarum has been hindered by its unique characteristics and biology, and difficulties in locating suitable populations of reference test animals. Infection status of fish in test populations is often unknown, and it is commonly assumed that the assay yielding the most positive results has the highest diagnostic accuracy, without consideration of misclassification of results. In...
Source: Journal of Fish Diseases - January 26, 2013 Category: Zoology Authors: D G Elliott, L J Applegate, A L Murray, M K Purcell, C L McKibben Tags: Original Article Source Type: research
Cross sections for bare and dressed carbon ions in water and neon.
Abstract The paper presents calculated cross sections for bare and dressed carbon projectiles of charge states q (0 to 6) with energies 1-10(4) keV u(-1) impacting on molecular water and atomic neon targets. The cross sections of water are of interest for radiobiological studies, but there are very few experimental data for water in any phase, while those for liquid water are non-existent. The more extensive experimental database for the neon target made it possible to test the reliability of the model calculations for the many-electron collision system. The current calculations cover major single and double elec...
Source: Physics in Medicine and Biology - January 26, 2013 Category: Physics Authors: Liamsuwan T, Nikjoo H Tags: Phys Med Biol Source Type: research
Functional characterization of ferret CCL20 and CCR6 and identification of chemotactic inhibitors.
Abstract CCL20 is currently the only known chemokine ligand for the receptor CCR6, and is a mucosal chemokine involved in normal and pathological immune responses. Although nucleotide sequence data are available for ccl20 and ccr6 sequences from multiple species, the ferret ccl20 and ccr6 sequences have not been determined. To increase our understanding of immune function in ferret models of infection and vaccination, we have used RT-PCR to obtain the ferret ccl20 and ccr6 cDNA sequences and functionally characterize the encoded proteins. The open reading frames of both genes were highly conserved across species an...
Source: Cytokine - January 26, 2013 Category: Molecular Biology Authors: Qin S, Klamar CR, Fallert Junecko BA, Craigo J, Fuller DH, Reinhart TA Tags: Cytokine Source Type: research
Nephronectin binds to heparan sulfate proteoglycans via its MAM domain.
Abstract Nephronectin is a basement membrane protein comprising five N-terminal epidermal growth factor (EGF)-like repeats, a central linker segment containing an Arg-Gly-Asp (RGD) motif and a C-terminal meprin-A5 protein-receptor protein tyrosine phosphatase μ (MAM) domain. Nephronectin has been shown to interact with α8β1 integrin through the central linker segment, but its interactions with other molecules remain to be elucidated. Here, we examined the binding of nephronectin to a panel of glycosaminoglycan (GAG) chains. Nephronectin bound strongly to heparin and chondroitin sulfate (CS)-E and moderately to h...
Source: Matrix Biology - January 26, 2013 Category: Molecular Biology Authors: Sato Y, Shimono C, Li S, Nakano I, Norioka N, Sugiura N, Kimata K, Yamada M, Sekiguchi K Tags: Matrix Biol Source Type: research
Disruption of heat shock factor 1 reduces the formation of conidia and thermotolerance in the mycoparasitic fungus Coniothyrium minitans.
Abstract Coniothyrium minitans is a bio-control agent of Sclerotinia spp., and has the ability to produce abundant conidia to infect the host fungi. Mediation of heat shock factors (HSFs) is required to adapt to the acute temperatures, and to regulate the expression of heat shock proteins (HSPs) to function as molecular chaperones to assist in development, protein folding and stability. A heat shock factor 1 (HSF1) gene was identified from a T-DNA insertion mutant that lost the ability to form conidia in liquid culture as well as on solid media. Null mutants lacking CmHSF1 were constructed by gene disruption strate...
Source: Fungal Genetics and Biology - January 26, 2013 Category: Biology Authors: Hamid MI, Zeng F, Cheng J, Jiang D, Fu Y Tags: Fungal Genet Biol Source Type: research
Receptors for the neuropeptides, myoinhibitory peptide and SIFamide, in control of the salivary glands of the blacklegged tick Ixodes scapularis.
In this study we identified authentic receptors expressed in the salivary glands for these neuropeptides. Homology-based searches for these receptors in the Ixodes scapularis genome sequence were followed by gene cloning and functional expression of the receptors. Both receptors were activated by low nanomolar concentrations of their respective ligands. The temporal expression patterns of the two ligands and their respective receptors suggest that the SIFamide signaling system pre-exists in unfed salivary glands, while the MIP system is activated upon initiation of feeding. Immunoreactivity for the SIFamide receptor in the...
Source: Insect Biochemistry and Molecular Biology - January 26, 2013 Category: Biochemistry Authors: Simo L, Koči J, Park Y Tags: Insect Biochem Mol Biol Source Type: research
Influence of extracellular pH on the cytotoxicity, cellular accumulation, and DNA interaction of novel pH-sensitive 2-aminoalcoholatoplatinum(II) complexes.
Abstract Extracellular acidity is a frequent pathophysiological condition of solid tumors offering possibilities for improving the tumor selectivity of molecular therapy. This might be accomplished by prodrugs with low systemic toxicity, attaining their full antitumor potency only under acidic conditions, such as bis(2-aminoalcoholato-κ²N,O)platinum(II) complexes that are activated by protonation of alcoholato oxygen, resulting in cleavage of platinum-oxygen bonds. In this work, we examined whether the pH dependency of such compounds is reflected in differential biological activity in vitro. In particular, the pH...
Source: Journal of Biological Inorganic Chemistry : JBIC - January 26, 2013 Category: Chemistry Authors: Valiahdi SM, Egger AE, Miklos W, Jungwirth U, Meelich K, Nock P, Berger W, Hartinger CG, Galanski M, Jakupec MA, Keppler BK Tags: J Biol Inorg Chem Source Type: research
A Novel Domain of Amino-Nogo-A Protects HT22 Cells Exposed to Oxygen Glucose Deprivation by Inhibiting NADPH Oxidase Activity.
In conclusion, TAT-M9 could be successfully transduced into HT22 cells, and protected HT22 cells against OGD damage by inhibiting NADPH oxidase-mediated oxidative stress. These findings suggest that the TAT-M9 protein may be an efficient therapeutic agent for neuroprotection. PMID: 23354671 [PubMed - as supplied by publisher]
Source: Cellular and Molecular Neurobiology - January 26, 2013 Category: Cytology Authors: Guo F, Wang H, Li L, Zhou H, Wei H, Jin W, Wang Q, Xiong L Tags: Cell Mol Neurobiol Source Type: research
Endothelial Tip Cells in Ocular Angiogenesis: Potential Target for Anti-Angiogenesis Therapy
Endothelial tip cells are leading cells at the tips of vascular sprouts coordinating multiple processes during angiogenesis. In the developing retina, tip cells play a tightly controlled, timely role in angiogenesis. In contrast, excessive numbers of tip cells are a characteristic of the chaotic pathological blood vessels in proliferative retinopathies. Tip cells control adjacent endothelial cells in a hierarchical manner to form the stalk of the sprouting vessel, using, among others, the VEGF-DLL-Notch signaling pathway, and recruit pericytes. Tip cells are guided toward avascular areas by signals from the local extracell...
Source: Journal of Histochemistry and Cytochemistry - January 26, 2013 Category: Biochemistry Authors: Siemerink, M. J., Klaassen, I., Van Noorden, C. J. F., Schlingemann, R. O. Tags: Review Source Type: research
Sulfated small molecules targeting EBV in Burkitt lymphoma: from in silico screening to the evidence of in vitro effect on viral episomal DNA
Abstract EBV infects more than 90% of the world population. Following primary infection, EBV persists in an asymptomatic latent state. Occasionally, it may switch to lytic infection. Latent EBV infection has been associated with several diseases, such as Burkitt lymphoma (BL). To date, there are no available drugs to target latent EBV and the existing broad spectrum antiviral drugs are mainly active against lytic viral infection. Thus, using computational molecular docking, a virtual screen of a library of small molecules, including xanthones and flavonoids (described with potential for antiviral activity against EBV), was...
Source: Chemical Biology and Drug Design - January 26, 2013 Category: Biology Authors: Raquel T. Lima, Hugo Seca, Andreia Palmeira, Miguel X. Fernandes, Felipe Castro, Marta Correia‐da‐Silva, Maria São José Nascimento, Emília Sousa, Madalena Pinto, M. HelenaVasconcelos Tags: Research Article Source Type: research
Experimental and quantum chemical studies of a novel synthetic prenylated chalcone
Conclusion: Considering all HF and DFT methods with GIAO calculations, TPSS and PBE1PBE were the most accurate methods used for calculation of 1H-NMR and 13C-NMR chemical shifts, which was almost similar to the B3LYP functional, followed in order by HF, M05-2X and M06-2X methods. All calculations were done using the Gaussian 09 software package. Theoretical calculations can be used to predict and confirm the structure of substituted chalcones with good correlation with the experimental data.
Source: Chemistry Central Journal - January 26, 2013 Category: Chemistry Authors: José Espinoza-HicksAlejandro Camacho-DávilaNorma Flores-HolguínGuadalupe Nevárez-MoorillónDaniel Glossman-MitnikLuz Rodríguez-Valdez Source Type: research
The wheat curl mite Aceria tosichella (Acari: Eriophyoidea) is a complex of cryptic lineages with divergent host ranges: evidence from molecular and plant bioassay data
Aceria tosichella (the wheat curl mite, WCM) is a global pest of wheat and other cereals, causing losses by direct damage, as well as the transmission of plant viruses. The mite is considered to have an unusually wide host range for an eriophyoid species. The present study tested the commonly held assumption that WCM is a single, highly polyphagous species by assessing the host range of genetically distinct lineages of WCM occurring in Poland on different host plants. Genotyping was performed by analyzing nucleotide sequence data from fragments of the mitochondrial cytochrome c oxidase subunit I (COI) and the nuclear D2 re...
Source: Biological Journal of the Linnean Society - January 25, 2013 Category: Research Authors: Anna Skoracka, Lechosław Kuczyński, Wiktoria Szydło, Brian Rector Tags: Research Article Source Type: research
Kinetic development of biofilm on NF membranes at the Méry-sur-Oise plant, France.
Abstract The kinetic formation of biofilms developing on nanofiltration (NF) membranes was studied for 2 years in the water production unit of Méry-sur-Oise, France. New membranes were set up in a pilot train integrated to the plant and autopsied after operation for 7, 80, 475 and 717 days. The biofouling layer was studied by confocal laser scanning microscope after 4',6-diamidino-2-phenyindole dihydrochloride and lectin staining, and by attenuated total reflectance-Fourier transform infrared spectroscopy and rheology experiments. Three stages of biofilm growth were discriminated: (1) the presence of sessile m...
Source: Biofouling - January 25, 2013 Category: Molecular Biology Authors: Houari A, Seyer D, Kecili K, Heim V, Martino PD Tags: Biofouling Source Type: research
Ruinous resident: the hydroid Ectopleura crocea negatively affects suspended culture of the mussel Mytilus galloprovincialis.
This study examined the impacts of E. crocea on mussel culture at two stages of the production cycle: spatfall and grow-out. Hydroids most commonly fouled the body, edge and dorsal regions of the mussel shell and cause a reduction in the length (4%) and weight (23%) of juvenile mussels. They also consumed mussel larvae in the field and in the laboratory. Prey numbers of many taxa, including mussel larvae, were consistent in natural hydroid diets regardless of the temporal variation in prey availability, implying some selectivity in hydroid feeding. In the laboratory, E. crocea consumed settling plantigrade mussel larvae mo...
Source: Biofouling - January 25, 2013 Category: Molecular Biology Authors: Fitridge I, Keough MJ Tags: Biofouling Source Type: research
Plumbing of hospital premises is a reservoir for opportunistically pathogenic microorganisms: a review.
Abstract Several bacterial species that are natural inhabitants of potable water distribution system biofilms are opportunistic pathogens important to sensitive patients in healthcare facilities. Waterborne healthcare-associated infections (HAI) may occur during the many uses of potable water in the healthcare environment. Prevention of infection is made more challenging by lack of data on infection rate and gaps in understanding of the ecology, virulence, and infectious dose of these opportunistic pathogens. Some healthcare facilities have been successful in reducing infections by following current water safety gu...
Source: Biofouling - January 25, 2013 Category: Molecular Biology Authors: Williams MM, Armbruster CR, Arduino MJ Tags: Biofouling Source Type: research
Morphometric and molecular identification of individual barnacle cyprids from wild plankton: an approach to detecting fouling and invasive barnacle species.
Abstract The present study used DNA barcodes to identify individual cyprids to species. This enables accurate quantification of larvae of potential fouling species in the plankton. In addition, it explains the settlement patterns of barnacles and serves as an early warning system of unwanted immigrant species. Sequences from a total of 540 individual cypris larvae from Taiwanese waters formed 36 monophyletic clades (species) in a phylogenetic tree. Of these clades, 26 were identified to species, but 10 unknown monophyletic clades represented non-native species. Cyprids of the invasive barnacle, Megabalanus cocopoma...
Source: Biofouling - January 25, 2013 Category: Molecular Biology Authors: Chen HN, Høeg JT, Chan BK Tags: Biofouling Source Type: research
Bacterial attachment on sub-nanometrically smooth titanium substrata.
This study demonstrated that the traditionally employed amplitudinal roughness parameters are not the only determinants of bacterial adhesion, and that spatial parameters can also be used to predict the extent of attachment. PMID: 23327438 [PubMed - in process]
Source: Biofouling - January 25, 2013 Category: Molecular Biology Authors: Webb HK, Boshkovikj V, Fluke CJ, Truong VK, Hasan J, Baulin VA, Lapovok R, Estrin Y, Crawford RJ, Ivanova EP Tags: Biofouling Source Type: research
Sunlight-enhanced calcareous deposition on cathodic stainless steel in natural seawater.
Abstract In replicate series of experiments in natural seawater, one in full darkness and the other in a 1:1 diurnal cycle with as little as ∼5% of natural solar illumination, sunlight promoted calcareous deposition on cathodic stainless steel surfaces. As exemplified by scanning electron microscopy, the deposit that formed under the natural diurnal cycle, in the presence of photosynthetic biofilms, was composed of finer calcareous crystals that provided more compact and more uniform surface coverage than the one formed in the dark. The light-enhanced deposit also possessed better scale properties, as suggested b...
Source: Biofouling - January 25, 2013 Category: Molecular Biology Authors: Eashwar M, Sathish Kumar P, Ravishankar R, Subramanian G Tags: Biofouling Source Type: research
The performance of aminoalkyl/fluorocarbon/hydrocarbon-modified xerogel coatings against the marine alga Ectocarpus crouaniorum: relative roles of surface energy and charge.
Abstract The effect of a series of xerogel coatings modified with aminoalkyl/fluorocarbon/hydrocarbon groups on the adhesion of a new test species, the filamentous brown alga Ectocarpus crouaniorum, has been explored, and compared with the green alga Ulva linza. The results showed that E. crouaniorum adhered weakly to the less polar, low wettability coatings in the series, but stronger adhesion was shown on polar, higher surface energy coatings containing aminoalkyl groups. The results from a separate series of coatings tuned to have similar surface energies and polarities after immersion in artificial seawater (AS...
Source: Biofouling - January 25, 2013 Category: Molecular Biology Authors: Evariste E, Gatley CM, Detty MR, Callow ME, Callow JA Tags: Biofouling Source Type: research
The effect of vibration frequency and amplitude on biofouling deterrence.
In this study, panels with embedded lead zirconate titanate, known as PZT, were placed in the field over 3 months. The panels were vibrated at different velocity levels at frequencies between 70 and 445 Hz. It was found that barnacles (Amphibalanus variegatus Darwin and Elminius sp.) were the only fouling organisms affected by the applied vibration, and these organisms settled in significantly lower numbers when the plates were excited at specific frequencies and amplitudes. Panels vibrating at relatively higher frequencies, greater than 260 Hz, exhibited reduced barnacle settlement, whilst lower frequencies in the 7...
Source: Biofouling - January 25, 2013 Category: Molecular Biology Authors: Choi CH, Scardino AJ, Dylejko PG, Fletcher LE, Juniper R Tags: Biofouling Source Type: research
Pathophysiology and molecular aspects of diffuse large B-cell lymphoma
Diffuse large B-Cell lymphoma is the most common subtype of non-Hodgkin lymphoma in the West. In Brazil, it is the fifth cause of cancer, with more than 55,000 cases and 26,000 deaths per year. At Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo - HCFMUSP, diffuse large B-Cell lymphoma represents 49.7% of all non-Hodgkin lymphoma cases. Initially, the classification of non-Hodgkin lymphoma was based on morphology, but advances in immunology and molecular medicine allowed the introduction of a biological classification for these diseases. As for other cancers, non-Hodgkin lymphoma involves patte...
Source: Revista Brasileira de Hematologia e Hemoterapia - January 25, 2013 Category: Hematology Source Type: research
Gerald R. Fink Elected 2014 AAAS President
Gerald R. Fink Chosen To Serve As AAAS President-Elect Fink, a pioneering molecular biologist and founding member of MIT’s Whitehead Institute, will begin his duties as the president-elect at the close of the 2013 Annual Meeting.
Source: AAAS - January 25, 2013 Category: Science Tags: Science News Source Type: research
Erratum to: GLP-1-dependent and independent effects and molecular mechanisms of a dipeptidyl peptidase 4 inhibitor in vascular endothelial cells.
PMID: 23271115 [PubMed - in process]
Source: Molecular Biology Reports - January 25, 2013 Category: Molecular Biology Authors: Hu Y, Liu H, Simpson RW, Dear AE Tags: Mol Biol Rep Source Type: research
[Essay] IBI* Series Winner: Keeping an Eye on Biology
Genomics Explorers, an IBI prize–winning module, engages students with bioinformatics and molecular research.Authors: Susan R. Singer, Jodi A. Schwarz, Cathryn A. Manduca, Sean P. Fox, Ellen R. Iverson, Benjamin J. Taylor, Steven B. Cannon, Gregory D. May, Sonja L. Maki, Andrew D. Farmer, Jeffrey J. Doyle
Source: Science: Current Issue - January 25, 2013 Category: Science Authors: Susan R. Singer Source Type: research
All's well that ends well: alternative polyadenylation and its implications for stem cell biology.
Abstract Stem cell quiescence, activation, and differentiation are governed by a complex network of molecular pathways. There has been a growing recognition that posttranscriptional modifications, such as alternative polyadenylation (APA) of transcripts, play an important role in regulating gene expression and function. Recent analyses of stem cell populations have suggested that APA controls stem cell fate and behavior. Here, we review recent developments that have shaped our understanding of the control of stem cell behavior by APA and we highlight promising areas for future investigation. PMID: 23357469 [Pub...
Source: Current Opinion in Cell Biology - January 25, 2013 Category: Cytology Authors: Mueller AA, Cheung TH, Rando TA Tags: Curr Opin Cell Biol Source Type: research
Transforming growth factor-β regulates the expression of anosmin (KAL-1) in human retinal pigment epithelial cells.
Abstract In a microarray analysis of human retinal pigment epithelial cells (HRPE) treated with TGF-β, in addition to the alteration of a number of known Extracellular matrix (ECM)-related genes regulated by TGF-β, we found a significant increase in the expression of Kallmann Syndrome (KAL)-1 gene, that codes for the protein anosmin-1. Enhanced expression of KAL-1 by TGF-β was validated by real-time PCR analysis. In in vitro experiments, TGF-β receptor inhibitor abolished TGF-β-induced expression of KAL-1. Immunofluorescence staining showed increased presence of anosmin-1 in TGF-β treated HRPE cells, with dis...
Source: Cytokine - January 25, 2013 Category: Molecular Biology Authors: Raju R, Jian B, Hooks JJ, Nagineni CN Tags: Cytokine Source Type: research
Cytokine profiles in acute myeloid leukemia patients at diagnosis: Survival is inversely correlated with IL-6 and directly correlated with IL-10 levels.
CONCLUSIONS: An aberrant production of the pro-inflammatory cytokines IL-6 and TNF-α and the anti-inflammatory cytokine IL-10 is observed in AML patients. Low levels of IL-6 and high levels of IL-10 represent favorable prognostic factors for survival in AML patients. These results support the idea that cytokine deregulation may be useful as a marker for predicting clinical evolution in AML patients. PMID: 23357299 [PubMed - as supplied by publisher]
Source: Cytokine - January 25, 2013 Category: Molecular Biology Authors: Sanchez-Correa B, Bergua JM, Campos C, Gayoso I, Arcos MJ, Bañas H, Morgado S, Casado JG, Solana R, Tarazona R Tags: Cytokine Source Type: research