Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 23.
Yap4 PKA- and GSK3-dependent phosphorylation affects its stability but not its nuclear localization
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Yap4 is a nuclear-resident transcription factor induced in Saccharomyces cerevisiae when exposed to several stress conditions, which include mild hyperosmotic and oxidative stress, temperature shift or metal exposure. This protein is also phosphorylated. Here we report that this modification is driven by PKA and GSK3. In order to ascertain whether Yap4 is directly or indirectly phosphorylated by PKA, we searched for stress and PKA-related kinases that could phosphorylate Yap4. We show that phosphorylation is independent of the kinases Rim15, Yak1, Sch9, Slt2, Ste20 and Ptk2. In addition, we showed that Yap4 phosphorylation...
Source: Yeast - September 21, 2009 Category: Molecular Biology Authors: Jorge Pereira, Catarina Pimentel, Catarina Amaral, Regina A. Menezes, Claudina Rodrigues-Pousada Source Type: journals
Prion-associated proteins in yeast: comparative analysis of isogenic [PSI+] and [psi-] strainsEmail this article to a colleague.
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A large group of prion-associated proteins was identified in yeast cells using a new approach, comparative analysis of pellet proteins of crude cell lysates in isogenic strains of Saccharomyces cerevisiae differing by their prion composition. Two-dimensional (2D) electrophoresis followed by MALDI analysis of the pellet proteins of [PSI+] and [psi-] strains after prion elimination by GuHCl and prion transmission by cytoduction permitted identification of ca. 40 proteins whose aggregation state correlated with the change of prion(s) content. Approximately half of these proteins belonged to chaperones and to enzymes of glucos...
Source: Yeast - September 21, 2009 Category: Molecular Biology Authors: Olga V. Nevzglyadova, Alexey V. Artemov, Alexey G. Mittenberg, Kirill V. Solovyov, Elena I. Kostyleva, Ekaterina V. Mikhailova, Irina M. Kuznetsova, Konstantin K. Turoverov, Tonu R. Soidla Source Type: journals
Substitution effect on the hydrofluorination reaction of unsaturated amines in superacid HF/SbF5Email this article to a colleague.
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Fei Liu, Agnes Martin-Mingot, Marie-Paule Jouannetaud, Omar Karam, Sebastien Thibaudeau
(Paper from Org. Biomol. Chem.)
Fei Liu, Org. Biomol. Chem., 2009, DOI: 10.1039/b915077c
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Source: RSC - Organic Biomolecular Chemistry - September 21, 2009 Category: Molecular Biology Authors: Fei LiuAgnes Martin-Mingot Marie-Paule Jouannetaud Omar Karam Sebastien Thibaudeau Source Type: journals
Molecular cloning and sexually dimorphic expression of DMRT4 gene in Oreochromis aureus.Email this article to a colleague.
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In this study, rapid amplification cDNA ends (RACE) was used for the isolation of DMRT4 full-length cDNA from the ovary of the blue tilapia Oreochromis aureus. The full-length of DMRT4 cDNA was 1,571 bp, containing the 148 bp 5'-untranslated region, 193 bp 3'-untranslated region and 1,230 bp open reading frame. The deduced amino acid sequence of the open reading frame (ORF) encoded a protein of 409 amino acids with a theoretical pI of 8.492 and a calculated molecular weight of 44.12 kDa. One conserved functional domain, DM-domain was identified in blue tilapia DMRT4. The DMRT4 full-length gene obtained from the blood was 1...
Source: Molecular Biology Reports - September 21, 2009 Category: Molecular Biology Authors: Cao J, Chen J, Wu T, Gan X, Luo Y Tags: Mol Biol Rep Source Type: journals
SPUD qPCR Assay Confirms PREXCEL-Q Softwares Ability to Avoid qPCR Inhibition.Email this article to a colleague.
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Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends. When removal of inhibitory substances by column or reagent-based methods fails or is incomplete, the remaining option of appropriately, precisely and differentially diluting samples and standards to non-inhibitory concentrations is often avoided due to the logistic problem it poses. To address this, we invented the PREXCEL-Q ...
Source: Current Issues in Molecular Biology - September 21, 2009 Category: Molecular Biology Authors: Gallup JM, Sow FB, Van Geelen A, Ackermann MR Tags: Curr Issues Mol Biol Source Type: journals
Epsilonx-vivo effect of dexamethasone on cytokine production from whole blood of septic patients: Correlation with disease severity.Email this article to a colleague.
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Conclusions: Part of the beneficiary effect of corticosteroids in sepsis may be due to an effect on the release of IL-1beta and of sTREM-1. This effect does not seem to be homogeneous for all septic patients.
PMID: 19783455 [PubMed - as supplied by publisher]
Source: Cytokine - September 21, 2009 Category: Molecular Biology Authors: Giamarellos-Bourboulis EJ, Dimopoulou I, Kotanidou A, Livaditi O, Pelekanou A, Tsagarakis S, Armaganidis A, Orfanos SE Tags: Cytokine Source Type: journals
Nucleation capacity and presence of centrioles define a distinct category of centrosome abnormalities that induces multipolar mitoses in cancer cellsEmail this article to a colleague.
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Analysis of centrosome number and structure has become one means of assessing the potential for aberrant chromosome segregation and aneuploidy in tumor cells. Centrosome amplification directly causes multipolar catastrophic mitoses in mouse embryonic fibroblasts (MEFs) deficient for the tumor suppressor genes Brca1 or Trp53. We observed supernumerary centrosomes in cell lines established from aneuploid, but not from diploid, colorectal carcinomas; however, multipolar mitoses were never observed. This discrepancy prompted us to thoroughly characterize the centrosome abnormalities in these and other cancer cell lines with re...
Source: Environmental and Molecular Mutagenesis - September 20, 2009 Category: Molecular Biology Authors: Michael J. Difilippantonio, B. Michael Ghadimi, Tamara Howard, Jordi Camps, Quang Tri Nguyen, Douglas K. Ferris, Dan L. Sackett, Thomas Ried Source Type: journals
Competitively selected protein ligands pay their increase in specificity by a decrease in affinityEmail this article to a colleague.
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Silke Hoffmann, Susanne Aileen Funke, Katja Wiesehan, Susanne Moedder, Julian Maurius Gluck, Sophie Feuerstein, Matthias Gerdts, Jessica Motter, Dieter Willbold
(Paper from Mol. BioSyst.)
Silke Hoffmann, Mol. BioSyst., 2009, DOI: 10.1039/b910945e
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Source: RSC - Mol. BioSyst. latest articles - September 20, 2009 Category: Molecular Biology Authors: Silke HoffmannSusanne Aileen Funke Katja Wiesehan Susanne Moedder Julian Maurius Gluck Sophie Feuerstein Matthias Gerdts Jessica Motter Dieter Willbold Source Type: journals
Neighbouring group participation vs. addition to oxacarbenium ions: studies on the synthesis of mycobacterial oligosaccharidesEmail this article to a colleague.
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Susanne A. Stalford, Colin A. Kilner, Andrew G. Leach, W. Bruce Turnbull
(Paper from Org. Biomol. Chem.)
Susanne A. Stalford, Org. Biomol. Chem., 2009, DOI: 10.1039/b914417j
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Source: RSC - Organic Biomolecular Chemistry - September 20, 2009 Category: Molecular Biology Authors: Susanne A. StalfordColin A. Kilner Andrew G. Leach W. Bruce Turnbull Source Type: journals
Olefin metathesis in carotenoid synthesisEmail this article to a colleague.
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Takayuki Kajikawa, Naoko Iguchi, Shigeo Katsumura
(Communication from Org. Biomol. Chem.)
Takayuki Kajikawa, Org. Biomol. Chem., 2009, DOI: 10.1039/b915390j
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Source: RSC - Organic Biomolecular Chemistry - September 20, 2009 Category: Molecular Biology Authors: Takayuki KajikawaNaoko Iguchi Shigeo Katsumura Source Type: journals
Rediscovering copper-based catalysts for intramolecular carbon-hydrogen bond functionalization by carbene insertionEmail this article to a colleague.
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Carmen Martin, Tomas R. Belderrain, Pedro J. Perez
(Paper from Org. Biomol. Chem.)
Carmen Martin, Org. Biomol. Chem., 2009, DOI: 10.1039/b911589g
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Source: RSC - Organic Biomolecular Chemistry - September 20, 2009 Category: Molecular Biology Authors: Carmen MartinTomas R. Belderrain Pedro J. Perez Source Type: journals
Cation localization and movement within DNA thrombin binding aptamer in solutionEmail this article to a colleague.
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Marko Trajkovski, Primoz Sket, Janez Plavec
(Paper from Org. Biomol. Chem.)
Marko Trajkovski, Org. Biomol. Chem., 2009, DOI: 10.1039/b914783g
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Source: RSC - Organic Biomolecular Chemistry - September 20, 2009 Category: Molecular Biology Authors: Marko TrajkovskiPrimoz Sket Janez Plavec Source Type: journals
Brain structure evolution in a basal vertebrate clade: evidence from phylogenetic comparative analysis of cichlid fishesEmail this article to a colleague.
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Conclusions:
Our results favor a mosaic model of brain evolution, as certain structures are evolving in a modular fashion, with a small but non-negligible influence of concerted evolution in cichlid fishes. Interestingly, one of the structures presenting distinct evolutionary patterns within cichlids, the olfactory bulbs, has also been shown to evolve differently from other structures in mammals. Hence, our results for a basal vertebrate clade also point towards a conserved developmental plan for all vertebrates.
Source: BMC Evolutionary Biology - Latest articles - September 20, 2009 Category: Molecular Biology Authors: Alejandro Gonzalez-VoyerSvante WinbergNiclas Kolm Source Type: journals
Evidence for a rapid rate of molecular evolution at the hypervariable and immunogenic Mycobacterium tuberculosis PPE38 gene regionEmail this article to a colleague.
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Conclusion:
Our results show that this antigen-encoding region of the M. tuberculosis genome is hypervariable. The observation that numerous different mutations have become fixed within specific lineages demonstrates that this genomic region is undergoing rapid molecular evolution and that further lineage-specific evolutionary expansion and diversification has occurred subsequent to the lineage-defining mutational events. The affected genes are not essential to the organism's virulence and we predict that their functional loss could aid immune evasion. Finally, we also show that the PPE38 region of the published M. tubercu...
Source: BMC Evolutionary Biology - Latest articles - September 20, 2009 Category: Molecular Biology Authors: Christopher McEvoyPaul van HeldenRobin WarrenNicolaas Gey van Pittius Source Type: journals
A Method for determining the dobustness of bio-molecular oscillator modelsEmail this article to a colleague.
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Conclusions:
The method here proposed for quantifying the robustness of biochemical oscillator models is computationally less demanding than similar multiparamter variation techniques available in the literature. It also provides tighter bounds on two models previously analyzed in the literature.
Source: BMC Systems Biology - Latest articles - September 20, 2009 Category: Molecular Biology Authors: Reza GhaemiJing SunPablo IglesiasDomitilla Del Vecchio Source Type: journals
Inferring Drosophila gap gene regulatory network:
a parameter sensitivity and perturbation analysisEmail this article to a colleague.
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Conclusions:
Parameter sensitivity analysis allows one to discriminate between circuits having significant parameter and qualitative differences but exhibiting the same quantitative pattern. Furthermore, we show that using a stochastic model derived from a deterministic solution, one can introduce fluctuations within the model to analyze the circuits' robustness. Ultimately, we show that there is a close relation between circuit sensitivity and robustness to fluctuation, and that circuit robustness is rather modular than global. The current study shows that reverse engineering of GRNs should not only focus on estimating pa...
Source: BMC Systems Biology - Latest articles - September 20, 2009 Category: Molecular Biology Authors: Yves Fomekong-NanfackMarten PostmaJaap Kaandorp Source Type: journals
Dynamics of virus spread in the presence of fluid flowEmail this article to a colleague.
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Samartha G. Anekal, Ying Zhu, Michael D. Graham, John Yin
(From Integr. Biol.)
Samartha G. Anekal, Integr. Biol., 2009, DOI: 10.1039/b908197f
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Source: RSC - Chemical Biology Virtual Journal latest articles - September 20, 2009 Category: Molecular Biology Authors: Samartha G. AnekalYing Zhu Michael D. Graham John Yin Source Type: journals
Free-energy relationships for the interactions of tryptophan with phosphocholinesEmail this article to a colleague.
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Georg Blaser, John M. Sanderson, Mark R. Wilson
(From Org. Biomol. Chem.)
Georg Blaser, Org. Biomol. Chem., 2009, DOI: 10.1039/b913919b
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Source: RSC - Chemical Biology Virtual Journal latest articles - September 20, 2009 Category: Molecular Biology Authors: Georg BlaserJohn M. Sanderson Mark R. Wilson Source Type: journals
Fluorescent detection of methylmercury by desulfurization reaction of rhodamine hydrazide derivativesEmail this article to a colleague.
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Young-Keun Yang, Sung-Kyun Ko, Injae Shin, Jinsung Tae
(Communication from Org. Biomol. Chem.)
Young-Keun Yang, Org. Biomol. Chem., 2009, DOI: 10.1039/b915723a
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Source: RSC - Organic Biomolecular Chemistry - September 20, 2009 Category: Molecular Biology Authors: Young-Keun YangSung-Kyun Ko Injae Shin Jinsung Tae Source Type: journals
In depth qualitative and quantitative profiling of tyrosine phosphorylation using a combination of phosphopeptide immuno-affinity purification and stable isotope dimethyl labeling.Email this article to a colleague.
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Several mass spectrometry based assays have emerged for the quantitative profiling of cellular tyrosine phosphorylation. Ideally, these methods should reveal the exact sites of tyrosine phosphorylation, be quantitative and not cost-prohibitive. The latter is often an issue as typically several milligrams of (stable isotope labeled) starting protein material is required to enable the detection of low abundant phosphotyrosine peptides. Here, we adopted and refined a peptide centric immuno-affinity purification approach for the quantitative analysis of tyrosine phosphorylation by combining it with a cost-effective stable ...
Source: Molecular and Cellular Proteomics : MCP - September 20, 2009 Category: Molecular Biology Authors: Boersema PJ, Foong LY, Ding VM, Lemeer S, van Breukelen B, Philp R, Boekhorst J, Snel B, den Hertog J, Choo AB, Heck AJ Tags: Mol Cell Proteomics Source Type: journals
Effects of integrins on laminin chemotaxis by hepatocellular carcinoma cells.Email this article to a colleague.
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To quantitatively evaluate the effects of integrins alpha1beta1, alpha2beta1, alpha3beta1, alpha4beta1, alpha5beta1, and alpha6beta1 on the chemotaxis of hepatocelluar carcinoma (HCC) cell line SMMC-7721 to laminin (LN). A modified dual-micropipette system was used to dynamically and quantitatively monitor the formation of pseudopod protrusion of HCC cells toward LN in the presence or absence of specific antibodies against integrins alpha1, alpha2, alpha3, alpha4, alpha5, alpha6, and beta1. Additionally, the expression levels of different integrin subunits on the surface of the cells were determined via flow cytometry ...
Source: Molecular Biology Reports - September 20, 2009 Category: Molecular Biology Authors: Fu BH, Wu ZZ, Qin J Tags: Mol Biol Rep Source Type: journals
Toll-like receptor signaling in cell proliferation and survival.Email this article to a colleague.
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Toll-like receptors (TLRs) are important sensors of foreign microbial components as well as products of damaged or inflamed self tissues. Upon sensing these molecules, TLRs initiate a series of downstream signaling events that drive cellular responses including the production of cytokines, chemokines, and other inflammatory mediators. This outcome results from the intracellular assembly of protein complexes that drive phosphorylation and other signaling cascades ultimately leading to chromatin remodeling and transcription factor activation. In addition to driving inflammatory responses, TLRs also regulate cell prolifer...
Source: Cytokine - September 20, 2009 Category: Molecular Biology Authors: Li X, Jiang S, Tapping RI Tags: Cytokine Source Type: journals
A method for determining the robustness of bio-molecular oscillator modelsEmail this article to a colleague.
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Conclusion:
The method here proposed for quantifying the robustness of biochemical oscillator models is computationally less demanding than similar multiparamter variation techniques available in the literature. It also provides tighter bounds on two models previously analyzed in the literature.
Source: BMC Systems Biology - Latest articles - September 20, 2009 Category: Molecular Biology Authors: Reza GhaemiJing SunPablo IglesiasDomitilla Del Vecchio Source Type: journals
Fine mapping of Plasmodium falciparum ribosomal phosphoprotein PfP0 revealed sequences with highly specific binding activity to human red blood cellsEmail this article to a colleague.
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In this study, 16 20-mer-long synthetic peptides spanning the entire PfP0 sequence were evaluated
by means of receptor–ligand assays with human red blood cells (RBCs) in order to determine the role played by these peptides
in the invasion process. Four RBC high-activity binding peptides (HABPs), located mostly toward the N-terminal region, were
identified: HABP 33898 (1MAKLSKQQKKQMYIEKLSSL20), HABP 33900 (41ASVRKSLRGKATILMGKNTRY60), HABP 33901 (61IRTALKKNLQAVPQIEKLLPY
80), and HABP 33906 (161LIKQGEKVTASSATLLRKFNY180). The binding pattern of HABPs 33898 and 33906 to enzyme-treated RBCs suggests receptors of protein na...
Source: Journal of Molecular Medicine - September 20, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
FRAP Analysis of Chemosensory Components of Dictyostelium.Email this article to a colleague.
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This study will lay out the methods of conducting FRAP experiments in Dictyostelium and discuss the large amount of knowledge which can be gained by adopting this as a common technique.
PMID: 19763979 [PubMed - in process]
Source: Mol Biol Cell - September 20, 2009 Category: Molecular Biology Authors: Elzie CA, Janetopoulos C Tags: Methods Mol Biol Source Type: journals
Top2 SUMO Conjugation in Yeast Cell Lysates.Email this article to a colleague.
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DNA topoisomerase II (Topo II), named Top2 in budding and fission yeast, is a conserved target of the SUMO modification pathway, with SUMO-conjugated forms of Topo II accumulating specifically during mitosis in both yeast and vertebrate cells (Bachant et al., Mol Cell 9, 1169-82, 2002; Azuma et al., J Cell Biol 163, 477-87, 2003; Dawlaty et al., Cell 133, 103-15, 2008). As with many SUMO substrates, the functional significance of this modification is still incompletely understood and, perhaps surprisingly, better characterized in vertebrates than yeasts. It seems likely, however, that continued analysis of yeast Top2 S...
Source: Mol Biol Cell - September 20, 2009 Category: Molecular Biology Authors: Baldwin M, Bachant J Tags: Methods Mol Biol Source Type: journals
Editorial note--Darwin and the origin of life.Email this article to a colleague.
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PMID: 19760521 [PubMed - in process]
Source: Origins of Life and Evolution of the Biosphere - September 20, 2009 Category: Molecular Biology Authors: Schwartz AW Tags: Orig Life Evol Biosph Source Type: journals
The coffee diterpene kahweol induces apoptosis in human leukemia U937 cells through down-regulation of Akt phosphorylation and activation of JNKEmail this article to a colleague.
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Abstract Kahweol, the coffee-specific diterpene, has been reported for its tumor cell growth inhibitory activity and anti-carcinogenic
activity. The mechanism by which kahweol initiates apoptosis remains poorly understood. In the present study, we investigated
the effect of kahweol on the apoptotic pathway in U937 human promonocytic cells. We show that kahweol induces apoptosis in
association with the activation of caspase 3 and cytochrome c release from the mitochondria to the cytosol, as well as down-regulation of anti-apoptotic proteins (Bcl-2, Bcl-xL, Mcl-1
and XIAP). Kahweol altered the phosphorylation ...
Source: Apoptosis - September 19, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Expression and SNP association analysis of porcine FBXL4 gene.Email this article to a colleague.
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As a kind of E3 ligase, the product of FBXL4 gene belongs to a member of FBLs which is the biggest eukaryotic subfamily of F-BOX proteins, it can recognize some substrate through particular protein-protein interaction domains. To investigate its functions, the polymorphism and association analysis was analyzed. The partial cDNA of porcine FBXL4 with 2384 bp long was first cloned; the deduced protein comprises a conserved F-BOX domain at position from the 277th to 332nd amino acid. The phylogenetic tree indicated porcine FBXL4 has the closest genetic relationship with bovine FBXL4 than other selected animal species. Ten...
Source: Molecular Biology Reports - September 18, 2009 Category: Molecular Biology Authors: Li Y, Yang SL, Tang ZL, Cui WT, Mu YL, Chu MX, Zhao SH, Wu ZF, Li K, Peng KM Tags: Mol Biol Rep Source Type: journals
TIM-3 as a new therapeutic target in systemic lupus erythematosus.Email this article to a colleague.
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T-cell immunoglobulin- and mucin-domain-containing molecule-3 (TIM-3) was the first surface molecule that specifically identifies Th1 cells in both mice and human. Recently, identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Many previous studies have demonstrated that TIM-3 influences chronic autoimmune diseases, such as multiple sclerosis and rheumatoid arthritis. In addition, association of TIM-3 polymorphisms with susceptibility to several autoimmune diseases has been identified. Recent work has explored ...
Source: Molecular Biology Reports - September 18, 2009 Category: Molecular Biology Authors: Pan HF, Zhang N, Li WX, Tao JH, Ye DQ Tags: Mol Biol Rep Source Type: journals
Increased circulating IL-8 is associated with reduced IGF-1 and related to poor metabolic control in adolescents with type 1 diabetes mellitus.Email this article to a colleague.
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Conclusions: In adolescents with T1DM and chronic, poor glucose control, increased serum IL-8 is associated with reduced IGF-1 suggesting a pro-inflammatory milieu that may contribute to alterations in the GH/IGF-1 axis.
PMID: 19773182 [PubMed - as supplied by publisher]
Source: Cytokine - September 18, 2009 Category: Molecular Biology Authors: Van Sickle BJ, Simmons J, Hall R, Raines M, Ness K, Spagnoli A Tags: Cytokine Source Type: journals
Lysosomal ceramide mediates gemcitabine-induced death of glioma cellsEmail this article to a colleague.
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Abstract Acid sphingomyelinase-induced ceramide release has been shown by many studies to induce apoptosis in response to various stimuli.
However, the mechanisms of acid sphingomyelinase/ceramide-mediated death signaling following treatment with chemotherapeutic
drugs have not been fully elucidated thus far. The present study demonstrates that treatment of glioma cells with clinically
achievable doses of gemcitabine results in acid sphingomyelinase activation, lysosomal accumulation of ceramide, cathepsin
D activation, Bax insertion into the mitochondria, and cell death. Pharmacological inhibition or geneti...
Source: Journal of Molecular Medicine - September 17, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Disease mutations in the “head” domain of the extra-sarcomeric protein desmin distinctly alter its assembly and network-forming propertiesEmail this article to a colleague.
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Abstract The intermediate filament protein desmin generates an extra-sarcomeric network in myocytes. Mutations in the desmin gene cause
myofibrillar myopathy characterized by desmin-positive aggregates and myofibrillar dissolution. Past analysis revealed that
the non-α-helical amino-terminal “head” domain of desmin is a vital coordinator of protein assembly. We have now characterized
assembly and network-forming properties of five recently discovered myopathy-causing mutations residing in this domain. In
vitro analyses with recombinant proteins show that two mutant variants residing in a conserved nonap...
Source: Journal of Molecular Medicine - September 17, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Post-translationally modified T cell epitopes: immune recognition and immunotherapyEmail this article to a colleague.
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Abstract The functionality of proteins is greatly extended by a diverse array of post-translational modifications (PTMs), many of which
are recognized by the immune system. Notably, a significant proportion of peptides presented to T cells by the major histocompatibility
complex in vivo are post-translationally modified. Since the cellular mechanisms that introduce and control protein modifications
can differ between health and disease, the associated changes in antigen presentation have the potential to alter immune responses.
A number of such situations have been implicated with infection, inflammation, au...
Source: Journal of Molecular Medicine - September 17, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
PerR vs OhrR: selective peroxide sensing in Bacillus subtilisEmail this article to a colleague.
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Victor Duarte, Jean-Marc Latour
(Review from Mol. BioSyst.)
Victor Duarte, Mol. BioSyst., 2009, DOI: 10.1039/b915042k
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Source: RSC - Mol. BioSyst. latest articles - September 17, 2009 Category: Molecular Biology Authors: Victor DuarteJean-Marc Latour Source Type: journals
Quadruple 9-mer-based protein binding microarray with DsRed fusion proteinEmail this article to a colleague.
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Conclusions:
Our method demonstrated PBM can be conveniently performed by adopting: (1) quadruple 9-mers may increase protein-DNA binding interactions in the microarray, and (2) a one-step incubation shortens the wash and hybridization steps. This technology will facilitate greater understanding of genome-wide interactions between proteins and DNA.
Source: BMC Molecular Biology - Latest articles - September 17, 2009 Category: Molecular Biology Authors: Min-Jeong KimTae-Ho LeeYoon-Mok PahkYul-Ho KimHyang-Mi ParkYang Do ChoiBaek Hie NahmYeon-Ki Kim Source Type: journals
The Sam domain of the lipid phosphatase Ship2 adopts a common model to interact with Arap3-Sam and EphA2-SamEmail this article to a colleague.
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Conclusions:
Our studies further clarify the structural features that are relevant for Sam-Sam interactions involving Ship2 and give additional hints that could be used for the identification of new molecules able to selectively inhibit Sam-Sam associations.
Source: BMC Structural Biology - Latest articles - September 17, 2009 Category: Molecular Biology Authors: Marilisa LeoneJason CellittiMaurizio Pellecchia Source Type: journals
Unbiased quantitation of Escherichia coli membrane proteome using phase-transfer surfactants.Email this article to a colleague.
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We developed a sample preparation protocol for rapid and unbiased analysis of the membrane proteome using an alimentary canal-mimicking system in which proteases are activated in the presence of bile salts. In this rapid and unbiased protocol, immobilized trypsin is used in the presence of deoxycholate and lauroylsarcocine to increase digestion efficiency, as well as to increase the solubility of the membrane proteins. Using 22.5 microgram of E. coli whole cell lysate, we quantitatively demonstrated that membrane proteins were extracted and digested at the same level as soluble proteins without any solubility-related b...
Source: Molecular and Cellular Proteomics : MCP - September 17, 2009 Category: Molecular Biology Authors: Masuda T, Saito N, Tomita M, Ishihama Y Tags: Mol Cell Proteomics Source Type: journals
The Role of GPI-anchored PrP(C) in Mediating the Neurotoxic Effect of Scrapie Prions in Neurons.Email this article to a colleague.
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There are two central phenomena in prion disease: prion replication and prion neurotoxicity. Underlying them both is the conversion of a host-encoded ubiquitously expressed protein, prion protein (PrP(C)), into a partially-protease resistant isoform, PrP(Sc), which accumulates in the brain. PrP(Sc) is associated with both pathology and infectivity. In the absence of PrP(C), PrP(Sc) cannot be generated and PrP-null mice do not propagate infectivity or develop pathology on infection with scrapie. However, while PrP(C) expression is fundamental to both prion infectivity and neurodegeneration, the uncoupling of these proce...
Source: Current Issues in Molecular Biology - September 17, 2009 Category: Molecular Biology Authors: Radford HE, Mallucci GR Tags: Curr Issues Mol Biol Source Type: journals
Targeting of the Prion Protein to the Cytosol: Mechanisms and Consequences.Email this article to a colleague.
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Prion diseases are characterized by the conformational transition of the cellular prion protein (PrP(C)) into an aberrant protein conformer, designated scrapie-prion protein (PrP(Sc)). A causal link between protein misfolding and neurodegeneration has been established for a variety of neurodegenerative disease, such as Alzheimer's disease, Parkinson's disease and polyglutamine diseases, but there is an ongoing debate about the nature of the neurotoxic species and how non-native conformers can damage neuronal populations. PrP is normally imported into the endoplasmic reticulum (ER) and targeted to the outer leaflet of t...
Source: Current Issues in Molecular Biology - September 17, 2009 Category: Molecular Biology Authors: Miesbauer M, Rambold AS, Winklhofer KF, Tatzelt J Tags: Curr Issues Mol Biol Source Type: journals
Prion Protein and Metal Interaction: Physiological and Pathological Implications.Email this article to a colleague.
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Metal induced free radicals are important mediators of neurotoxicity in several neurodegenerative conditions such as Alzheimer's disease, Parkinson's disease, and Huntington's disease. Similar evidence is now emerging for prion diseases, a group of neurodegenerative disorders of humans and animals. The main pathogenic agent in all prion disorders is PrP-scrapie (PrP(Sc)), a beta-sheet rich isoform of a normal cell surface glycoprotein known as the prion protein (PrP(C)). Deposits of PrP(Sc) in the brain parenchyma are believed to induce neurotoxicity through poorly understood mechanisms. Recent reports suggest that imb...
Source: Current Issues in Molecular Biology - September 17, 2009 Category: Molecular Biology Authors: Singh N, Das D, Singh A, Mohan ML Tags: Curr Issues Mol Biol Source Type: journals
Autophagy, Prion Infection and their Mutual Interactions.Email this article to a colleague.
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Prion diseases are infectious and fatal neurodegenerative disorders of man and animals which are characterized by spongiform degeneration in the central nervous system. Prion propagation involves the endocytic pathway and endosomal and lysosomal compartments are implicated in trafficking and re-cycling as well as final degradation of prions. Shifting the equilibrium between propagation and lysosomal clearance to the latter impairs cellular prion load. This and earlier findings of autophagic vacuoles in correlation to prion infections both in in vitro and in vivo studies prompted us and others to analyze the role of aut...
Source: Current Issues in Molecular Biology - September 17, 2009 Category: Molecular Biology Authors: Heiseke A, Aguib Y, Schatzl HM Tags: Curr Issues Mol Biol Source Type: journals
Prion Protein: Orchestrating Neurotrophic Activities.Email this article to a colleague.
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PrP(C) is highly expressed in both the central and peripheral nervous systems from early stages of development and in adulthood. Its major conformational change and conversion into an abnormal form (PrP(Sc)) has been associated with the generation of prions, the infectious agent of transmissible spongiform encephalopathies (TSEs). The massive neurodegeneration presented by individuals suffering from these diseases has been associated with the gain of neurotoxic activity of PrP(Sc). On the other hand, major neurodegeneration is also observed in transgenic mice expressing PrP(C) molecules deleted of specific domains, whi...
Source: Current Issues in Molecular Biology - September 17, 2009 Category: Molecular Biology Authors: Martins VR, Beraldo FH, Hajj GN, Lopes MH, Lee KS, Prado MM, Linden R Tags: Curr Issues Mol Biol Source Type: journals
Prion Neurotoxicity: Insights from Prion Protein Mutants.Email this article to a colleague.
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The chemical nature of prions and the mechanism by which they propagate are now reasonably well understood. In contrast, much less is known about the identity of the toxic prion protein (PrP) species that are responsible for neuronal death, and the cellular pathways that these forms activate. In addition, the normal, physiological function of cellular PrP (PrP(C)) has remained mysterious, hampering efforts to determine whether loss or alteration of this function contributes to the disease phenotype. Considerable evidence now suggests that aggregation, toxicity, and infectivity are distinct properties of PrP that do no ...
Source: Current Issues in Molecular Biology - September 17, 2009 Category: Molecular Biology Authors: Solomon IH, Schepker JA, Harris DA Tags: Curr Issues Mol Biol Source Type: journals
The Prion Protein: Friend and Foe.Email this article to a colleague.
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A conformational transition of the cellular prion protein (PrP(C)) into an aberrantly folded isoform designated scrapie prion protein (PrP(Sc)) is the hallmark of a variety of neurodegenerative disorders collectively called prion diseases. They include Creutzfeldt-Jakob disease and Gerstmann-Staussler-Scheinker syndrome in humans, scrapie in sheep, bovine spongiform encephalopathy (BSE) in cattle and chronic wasting disease (CWD) in free-ranging deer. In contrast to the deadly properties of misfolded PrP, PrP(C) seems to possess a neuroprotective activity. More-over, animal models indicated that the stress-protective a...
Source: Current Issues in Molecular Biology - September 17, 2009 Category: Molecular Biology Authors: Tatzelt J Tags: Curr Issues Mol Biol Source Type: journals
Bone Marrow-Derived Cells in the Tumor Microenvironment Contain Cells with Primitive Hematopoietic Phenotype.Email this article to a colleague.
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Abstract Infiltration of bone marrow-derived cells is part of the angiogenic switch required for uncontrolled tumor growth. However, the nature of the tumor-infiltrating cells from bone marrow has not been fully elucidated. To investigate the phenotype of bone marrow-derived cells within a tumor, we employed the Lewis Lung Carcinoma (LLC) murine tumor model. We followed bone marrow-derivation of tumor-infiltrating cells through transplantation of CD45.2 bone marrow cells into pre-irradiated CD45.1 mice. We found robust CD45.2 donor type chimerism in bone marrow and blood of CD45.1 recipient tumor bearing mice. Flow cyt...
Source: J Cell Mol Med - September 17, 2009 Category: Molecular Biology Authors: Deak E, Göttig S, Rüster B, Paunescu V, Seifried E, Gille J, Henschler R Tags: J Cell Mol Med Source Type: journals
Anti-CD44 induces apoptosis in T lymphoma via mitochondrial depolarization.Email this article to a colleague.
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Abstract A blockade of CD44 can interfere with hematopoietic and leukemic stem cell homing, the latter being considered as a therapeutic option in hematological malignancies. We here aimed to explore the molecular mechanism underlying the therapeutic efficacy of anti-CD44. We noted that in irradiated mice reconstituted with a bone marrow cell transplant, anti-CD44 exerts a stronger effect on hematopoietic reconstitution than on T lymphoma (EL4) growth. Nonetheless, in the non-reconstituted mouse anti-CD44 suffices for a prolonged survival of EL4-bearing mice, where anti-CD44-prohibited homing actively drives EL4 cells ...
Source: J Cell Mol Med - September 17, 2009 Category: Molecular Biology Authors: Rajasagi M, von Au A, Singh R, Hartmann N, Zöller M, Marhaba R Tags: J Cell Mol Med Source Type: journals
BioEssays 10/2009Email this article to a colleague.
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Cover Photograph: A superposition of a fish with colour patterning produced by a gene expression network involving a "paradoxical" relationship between two genes, of the type A inhibits B; B activates A, upon a computer simulation of the same. When explored in terms of spatial resolution, apparent paradoxes represented by simple connectivity diagrams produce undulating patterns; in other situations they produce temporal oscillations. To read more on this topic, see the article by Mark Isalan on pages 1110-1115. Fish image reproduced with kind permission from Anka Zolnierzak; computer simulation image reproduced with kind p...
Source: BioEssays - September 17, 2009 Category: Molecular Biology Tags: Cover Picture Source Type: journals
NDRG1 and CRK-I/II are regulators of endothelial cell migration under intermittent hypoxiaEmail this article to a colleague.
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Abstract Intermittent Hypoxia (IH) that develops in neovascularized solid tumours has been described to positively influence the tumour
growth by modulating the behaviour of cancer cells as well as of endothelial cells. However, the molecular mechanisms regulated
by IH still remain poorly understood. In this work, the effects of IH were investigated on endothelial cells by a proteomic
approach. Protein abundance variations were studied using fluorescent 2D-Differential in Gel Electrophoresis (2D-DIGE). Amongst
the proteins of which the abundance varied under IH, NDRG1 and CRK-I/II were identified by mass spe...
Source: Angiogenesis - September 17, 2009 Category: Molecular Biology Tags: Angiogenesis Source Type: journals
Benzyl isothiocyanate exhibits anti-inflammatory effects in murine macrophages and in mouse skinEmail this article to a colleague.
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This study examined whether BITC inhibits lipopolysaccharide (LPS)-induced inflammatory responses in Raw 264.7 macrophages
and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema formation. The treatment of macrophages with various concentrations
of BITC resulted in a dose-dependent reduction in the LPS-induced secretion of interleukin (IL)-1β, TNF-α, and IL-6 and their
corresponding mRNA levels, as well as in the production of nitric oxide and PGE2. Consistent with these findings, BITC inhibited the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and
cyclooxygenase (COX)-2 proteins and the...
Source: Journal of Molecular Medicine - September 17, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
