Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 24.
CCMV capsid formation induced by a functional negatively charged polymer
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Inge J. Minten, Yujie Ma, Mark A Hempenius, G. Julius Vancso, Roeland J. M. Nolte, Jeroen J. L. M. Cornelissen
(Paper from Org. Biomol. Chem.)
Inge J. Minten, Org. Biomol. Chem., 2009, DOI: 10.1039/b915028e
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The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - September 16, 2009 Category: Molecular Biology Authors: Inge J. MintenYujie Ma Mark A Hempenius G. Julius Vancso Roeland J. M. Nolte Jeroen J. L. M. Cornelissen Source Type: journals
The reaction of acyl cyanides with "Huisgen zwitterion": an interesting rearrangement involving ester group migration between oxygen and nitrogen atomsEmail this article to a colleague.
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Xu-Guang Liu, Yin Wei, Min Shi
(Paper from Org. Biomol. Chem.)
Xu-Guang Liu, Org. Biomol. Chem., 2009, DOI: 10.1039/b913196e
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Source: RSC - Organic Biomolecular Chemistry - September 16, 2009 Category: Molecular Biology Authors: Xu-Guang LiuYin Wei Min Shi Source Type: journals
Two novel cSNPs of weaver gene in Chinese indigenous goat and their associations with milk yield.Email this article to a colleague.
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The objective of this study was to detect the genetic variation of five loci within weaver gene by PCR-SSCP, DNA sequencing and forced PCR-RFLP methods in 1,019 Chinese indigenous goats. Two novel coding SNPs (XM_598993:m.864G>A; XM_598993:m.1224T>A) locating on P3 and P4 loci were identified and detected by MluI and AsuII forced PCR-RFLP, respectively. In the MluI analysis, the frequencies of goat MluI-A allele in the analyzed populations were 0.226, 0.248, 0.096 and 0.088 for XNSN, GZ, SBWC and XJWC, respectively. Genotypic frequencies were found to be significantly different in four breeds (chi(2) = 75.842, df = 6...
Source: Molecular Biology Reports - September 16, 2009 Category: Molecular Biology Authors: Li Z, Chen Z, Lan X, Ma L, Qu Y, Liu Y, Li M, Wang P, Li F, Chen H Tags: Mol Biol Rep Source Type: journals
Role of aldose reductase in TGF-beta1-induced fibronectin synthesis in human mesangial cells.Email this article to a colleague.
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In this study, we explored the role of AR in FN production and possible mechanism involved. The expression of AR, FN and c-Jun proteins were analyzed by Western blot and the activity of activator protein-1 (AP-1) was assessed by electrophoretic mobility shift assay (EMSA). Our results showed that AR could mediate the TGF-beta1-induced FN production, which may associate with AP-1 activation.
PMID: 19760097 [PubMed - as supplied by publisher]
Source: Molecular Biology Reports - September 16, 2009 Category: Molecular Biology Authors: Zhang Y, Huang P, Jiang T, Zhao J, Zhang N Tags: Mol Biol Rep Source Type: journals
Two novel SNPs in the coding region of the bovine PRDM16 gene and its associations with growth traits.Email this article to a colleague.
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As a zinc-finger protein, PR domain containing 16 (PRDM16) controls brown fat determination by stimulating brown fat-selective genes expression while suppressing the expression of genes selective for white fat cells, whose mutations were associated with myelodysplastic syndrome (MDS) and leukemogenesis in human and murine model of leukemia. To date, no polymorphisms of PRDM16 gene in bovine had been reported. Herein, PCR-SSCP and DNA sequencing methods were employed to screen the genetic variation within PRDM16 gene in 1031 Chinese indigenous bovine. The results revealed two novel silent mutations: XM_001788152: m.1641...
Source: Molecular Biology Reports - September 16, 2009 Category: Molecular Biology Authors: Wang J, Li ZJ, Lan XY, Hua LS, Huai YT, Huang YZ, Ma L, Zhao M, Jing YJ, Chen H, Wang JQ Tags: Mol Biol Rep Source Type: journals
Analysis of genetic diversity in cytoplasmic male sterility, and association of mitochondrial genes with petaloid-type cytoplasmic male sterility in tuber mustard (Brassica juncea var. tumida Tsen et Lee).Email this article to a colleague.
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In our previous study, we bred a stable cytoplasmic male sterility (CMS) line of tuber mustard by using distant hybridization and subsequent backcrosses. In this CMS plants, all floral organs are normal except the anthers, which are transformed into petals or tubular structures. Recently, 2 mitochondrial genes-atpA and orf220-that are distinctively present in the CMS line of tuber mustard were cloned and partially characterized. In our study of genetic diversity analysis of CMS, 7 species of Brassica and Raphanus crops, which included 5 CMS lines and their respective maintainer lines, were used to compare the constitut...
Source: Molecular Biology Reports - September 16, 2009 Category: Molecular Biology Authors: Yu X, Lu H, Lu G, Chen Z, Cao J, Hirata Y Tags: Mol Biol Rep Source Type: journals
Sequence analysis of an isolate of minute virus of canines in China reveals the closed association with bocavirus.Email this article to a colleague.
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In the present study, we have cloned and sequenced the nearly-full-length genome of minute virus of canines (MVC), SH26, in China. The genome of MVC, 5,132 nucleotides (nts) in length, contains three open reading frames (ORFs), which are 2,325-bp of NS1, 561-bp of NP1 and 2,112-bp of VP1/VP2 encoding three proteins of 774, 186 and 703 residues, respectively. Predicted amino acids sequence of NS1 of MVC has 44% identity with human bocavirus (HBoV) and human boacvirus 2 (HBoV2), NP1 has 48 and 45% identity with HBoV and HBoV2, VP1/VP2 has 45 and 46% identity with HBoV and HBoV2, respectively. Phylogenetic analysis showed...
Source: Molecular Biology Reports - September 16, 2009 Category: Molecular Biology Authors: Shan TL, Cui L, Dai XQ, Guo W, Shang XG, Yu Y, Zhang W, Kang YJ, Shen Q, Yang ZB, Zhu JG, Hua XG Tags: Mol Biol Rep Source Type: journals
Molecular cloning and mRNA expression of peroxiredoxin gene in black tiger shrimp (Penaeus monodon).Email this article to a colleague.
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The techniques of homology cloning and anchored PCR were used to clone the peroxiredoxin (Prx) gene from black tiger shrimp (Penaeus monodon). The full length cDNA of black tiger shrimp Prx (PmPrx) contained a 5' untranslated region (UTR) of 51 bp, an ORF (open reading frame) of 582 bp encoding a polypeptide of 193 amino acids with an estimated molecular mass of 22.15 kDa and a 3' UTR of 948 bp. Sequence comparison showed that PmPrx shared higher identities with Prx IVs than that with other isoforms of Prx, indicating PmPrx was a member of the Prx IV family. A quantitative reverse transcriptase Real-Time PCR (qRT-PCR) ...
Source: Molecular Biology Reports - September 16, 2009 Category: Molecular Biology Authors: Qiu L, Ma Z, Jiang S, Wang W, Zhou F, Huang J, Li J, Yang Q Tags: Mol Biol Rep Source Type: journals
Impaired wound healing in mice lacking the basement membrane protein nidogen 1.Email this article to a colleague.
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Nidogen 1 and 2 are ubiquitous basement membrane (BM) components, whose interactions in particular with laminin, collagen IV and perlecan have been considered important for BM formation. Genetic deletion of either NID gene does not reveal BM alterations suggesting compensatory roles for nidogen 1 and 2. However, neurological deficits in nidogen 1 null mice, not seen in the absence of nidogen 2, also suggest isoform specific functions. To test this further, skin wound healing which requires BM reformation was studied in adult nidogen 1 deficient mice. Although re-epithelization was not altered, the newly formed epidermi...
Source: Matrix Biology - September 16, 2009 Category: Molecular Biology Authors: Baranowsky A, Mokkapati S, Bechtel M, Krügel J, Miosge N, Wickenhauser C, Smyth N, Nischt R Tags: Matrix Biol Source Type: journals
Computer-aided quantification of retinal neovascularizationEmail this article to a colleague.
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Abstract Rodent models of retinal angiogenesis play a pivotal role in angiogenesis research. These models are a window to developmental
angiogenesis, to pathological retinopathy, and are also in vivo tools for anti-angiogenic drug screening in cancer and ophthalmic
research. The mouse model of oxygen-induced retinopathy (OIR) has emerged as one of the leading in vivo models for these purposes.
Many of the animal studies that laid the foundation for the recent breakthrough of anti-angiogenic treatments into clinical
practice were performed in the OIR model. However, readouts from the OIR model have ...
Source: Angiogenesis - September 16, 2009 Category: Molecular Biology Tags: Angiogenesis Source Type: journals
Efficient synthesis of the C7-C20 subunit of amphidinolides C and FEmail this article to a colleague.
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Subham Mahapatra, Rich G. Carter
(Communication from Org. Biomol. Chem.)
Subham Mahapatra, Org. Biomol. Chem., 2009, DOI: 10.1039/b916744g
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Source: RSC - Organic Biomolecular Chemistry - September 15, 2009 Category: Molecular Biology Authors: Subham MahapatraRich G. Carter Source Type: journals
The new era of 1,2,4-oxadiazolesEmail this article to a colleague.
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Andrea Pace, Paola Pierro
(Perspective from Org. Biomol. Chem.)
Andrea Pace, Org. Biomol. Chem., 2009, DOI: 10.1039/b908937c
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Source: RSC - Organic Biomolecular Chemistry - September 15, 2009 Category: Molecular Biology Authors: Andrea PacePaola Pierro Source Type: journals
Synthesis of functionalized H-pyrazolo[5,1-a]isoquinolines via sequential reactions of N[prime or minute]-(2-alkynylbenzylidene)hydrazidesEmail this article to a colleague.
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Zhiyuan Chen, Mingchao Su, Xingxin Yu, Jie Wu
(Paper from Org. Biomol. Chem.)
Zhiyuan Chen, Org. Biomol. Chem., 2009, DOI: 10.1039/b914265g
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Source: RSC - Organic Biomolecular Chemistry - September 15, 2009 Category: Molecular Biology Authors: Zhiyuan ChenMingchao Su Xingxin Yu Jie Wu Source Type: journals
Exploration of chromatic aberration for multiplanar imaging: Proof of concept with implications for fast, efficient autofocusEmail this article to a colleague.
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Image-based autofocus determines focus directly from the specimen (as opposed to reflective surface positioning with an offset), but sequential acquisition of a stack of images to measure resolution/sharpness and find best focus is slower than reflective positioning. Simultaneous imaging of multiple focal planes, which is also useful for 3D imaging of live cells, is faster but requires complicated optics. With color CCD cameras and white light sources commonly available, we asked if axial chromatic aberration can be utilized to acquire multiple focal planes simultaneously, and if it can be controlled through a range suffic...
Source: Cytometry Part A - September 15, 2009 Category: Molecular Biology Authors: Martin Weinigel, Albert L. Kellner, Jeffrey H. Price Source Type: journals
Characterization of bovine miRNAs by sequencing and bioinformatics analysisEmail this article to a colleague.
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Conclusions:
The miRNA expression patterns among 11 tissues from beef cattle revealed that most miRNAs were ubiquitously expressed in all tissues, while only a few miRNAs were tissue specific. Only 60% miRNAs in this study were found to display strand bias, suggesting that there are some key factors for mature miRNA selection other than internal stability. Most bovine miRNAs are highly conserved in other three mammalian species, indicating that these miRNAs may have a role in different species that are potential molecular markers for evolution.
Source: BMC Molecular Biology - Latest articles - September 15, 2009 Category: Molecular Biology Authors: Weiwu JinJason GrantPaul StothardStephen MooreLe Luo Guan Source Type: journals
Synthetic lethal targeting of PTEN mutant cells with PARP inhibitorsEmail this article to a colleague.
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The tumour suppressor gene, phosphatase and tensin homolog (PTEN), is one of the most commonly mutated genes in human cancers. Recent evidence suggests that PTEN is important for the maintenance of genome stability. Here, we show that PTEN deficiency causes a homologous recombination (HR) defect in human tumour cells. The HR deficiency caused by PTEN deficiency, sensitizes tumour cells to potent inhibitors of the DNA repair enzyme poly(ADP-ribose) polymerase (PARP), both in vitro and in vivo. PARP inhibitors are now showing considerable promise in the clinic, specifically in patients with mutations in either of the breast ...
Source: EMBO Molecular Medicine - September 15, 2009 Category: Molecular Biology Authors: Ana M. Mendes-Pereira, Sarah A. Martin, Rachel Brough, Afshan McCarthy, Jessica R. Taylor, Jung-Sik Kim, Todd Waldman, Christopher J. Lord, Alan Ashworth Source Type: journals
Characterization of gene expression of QM from Caragana jubata, a plant species that grows under extreme cold.Email this article to a colleague.
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Caragana [Caragana jubata (Pall.) Poir] is a temperate plant that thrives well under extremes of cold in high altitude of Himalaya and hence the plant is expected to be a source of genes that might play an important role in tolerance to low temperature (LT). In order to identify LT inducible gene(s), differential display of mRNA (DD) was performed using the apical buds growing under snow as well as growing in the near vicinity without snow, and a LT inducible QM gene (CjQM) homologue was identified. Realizing the importance of QM gene (which encodes human Wilms' tumor suppressor QM protein) in aggregation of 40 and 60S...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Bhardwaj PK, Ahuja PS, Kumar S Tags: Mol Biol Rep Source Type: journals
beta-Ionone-induced apoptosis in human osteosarcoma (U2os) cells occurs via a p53-dependent signaling pathway.Email this article to a colleague.
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In this study, we determined whether beta-ionone can induce apoptosis in U2os cells in vitro and which signal pathway(s) is involved. We found that beta-ionone inhibited cell proliferation in U2os cells in a concentration- and time-dependent manner and caused cell cycle arrest at the G1-S phase. TUNEL assay, DNA ladder and assessment of Caspase 3 activity showed that apoptosis was the determinant in the effects of beta-ionone. Furthermore, Expression of the p53 protein increased in a concentration-dependent and time-dependent manner according to immunocytochemistry and immunoblotting after beta-ionone treatment. In additio...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Zhu J, Zhang L, Jin X, Han X, Sun C, Yan J Tags: Mol Biol Rep Source Type: journals
Phylogenetic analysis of 16S rRNA gene sequences reveals rumen bacterial diversity in Yaks (Bos grunniens).Email this article to a colleague.
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Six matured male Yaks (Bos grunniens) with a mean live weight of 450 +/- 23 kg (mean +/- SD), were housed indoors in metabolism cages and fed pelleted lucerne (Medicago sativum). After an adjustment period of 24 days of feeding the diet, samples of rumen content were obtained for analysis of the bacteria in the liquor. The diversity of rumen bacteria was investigated by constructing a 16S rRNA gene clone library using the general bacterial primers F27 and R1492. A total of 130 clones, comprising nearly full length sequences (approx. 1.5 kb) were sequenced and submitted to BLAST and phylogenetic analysis. Using the crit...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Yang LY, Chen J, Cheng XL, Xi DM, Yang SL, Deng WD, Mao HM Tags: Mol Biol Rep Source Type: journals
Differential expression of a novel gene BRE (TNFRSF1A modulator/BRCC45) in response to stress and biological signals.Email this article to a colleague.
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Stress-responsive genes play critical roles in many biological functions that includes apoptosis, survival, differentiation and regeneration. We have identified a novel stress-responsive gene called BRE which interacts with TNF-receptor-1 and blocks the apoptotic effect of TNF-alpha. BRE enhances tumor growth in vivo and is up-regulated in hepatocellular and esophageal carcinomas. BRE also regulates the ubiquitination of the DNA repair complex BRCC, and the synthesis of steroid hormones. Here, we examined BRE-mRNA in cells after treatments with UV and ionizing radiation (IR). UV and IR treatment alone suppressed BRE-mR...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Chan JY, Li L, Miao J, Cai DQ, Lee KK, Chui YL Tags: Mol Biol Rep Source Type: journals
The role of lysophosphatidic acid receptors in phenotypic modulation of vascular smooth muscle cells.Email this article to a colleague.
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In this study we detect the expression of LPA1 and LPA3 in rat aortic smooth muscle cells (RASMCs). LPA promoted RASMCs phenotypic modulation in a dose-dependent manner and coordinated induced the phosphorylation of p38 mitogen-activated protein kinase (p38MAPK) and extracellular signal-regulated kinase (ERK). LPA-induced cell phenotypic modulation was significantly inhibited by specific LPA1/LPA3-receptor antagonist dioctyl-glycerol pyrophosphate (DGPP8:0) at concentration, but this inhibitive effect was lost when the antagonist was coadministered with a highly selective LPA3 agonist,1-oleoyl-2-Omethyl-rac-glycero-phospho...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Zhou Z, Niu J, Zhang Z Tags: Mol Biol Rep Source Type: journals
Codon optimization of cry1Ab gene for hyper expression in plant organelles.Email this article to a colleague.
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With the advent of genetic manipulation techniques, it has become possible to clone and insert gene into the genome of crop plants to confer resistance to insects and pests. Resistance to insects has been demonstrating in transgenic plants either by triggering defense system of plants or by expressing heterologous cry genes for delta-endotoxins from Bacillus thuringiensis. In the present study, synthetic cry1Ab gene was developed with optimized chloroplast preferred codons and is expressed in tobacco plastid genome called plastome, following chloroplast transformation strategy, which is environment friendly technique t...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Jabeen R, Khan MS, Zafar Y, Anjum T Tags: Mol Biol Rep Source Type: journals
Maggot excretions/secretions induces human microvascular endothelial cell migration through AKT1.Email this article to a colleague.
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Maggot therapy is a simple and highly successful method for healing of infected and necrotic wounds. The increasing evidences indicate that Maggot excretions/secretions (ES) plays important roles in the wounds healing process. But the precise molecular mechanisms remain undefined. Herein, we investigated if ES induced cell migration during wound healing process using microvascular endothelial cells (HMEC-1) as model, and this effect was associated with the activation of AKT1 and ERK1/2. Wound healing and transwell migration assays were performed to study the effects of ES on HMEC-1 cell migration. Our data showed that ...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Wang SY, Wang K, Xin Y, Lv DC Tags: Mol Biol Rep Source Type: journals
A hybrid promoter-containing vector for direct cloning and enhanced expression of PCR-amplified ORFs in mammalian cells.Email this article to a colleague.
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An efficient vector, designated as pCAGX, was designed for direct cloning and enhanced expression of PCR-amplified ORFs in mammalian cells. It relied on the well-known TA-cloning principle, and utilized the CMV enhancer/chicken beta-actin/rabbit beta-globin (CAG) hybrid promoter instead of the classical CMV promoter to drive more efficient transgene expression in wider host cells. The specially designed cassette under CAG hybrid promoter contained two tandemly arrayed XcmI sites which were spaced by an additional EcoRV site. For direct cloning and expressing PCR-amplified ORFs, the T-vector was prepared by further dige...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: You LM, Luo J, Wang AP, Zhang GP, Weng HB, Guo YN, Liu YC, Li QM, Teng M Tags: Mol Biol Rep Source Type: journals
Transcriptional regulation of human mucin gene MUC4 in pancreatic cancer cells.Email this article to a colleague.
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In this report, we used pancreatic cancer cell line (Bxpc-3) to explore the potential transcription factors regulating MUC4 transcriptional activity. Through promoter screening, overexpressing methods and luciferase reporter studies, we found that transcription factors CREB, Ets-1, Elk-1 and STAT1 can positively regulate MUC4 expression at the promoter and mRNA level. Our findings will be helpful for better understanding the transcriptional regulation of MUC4 in pancreatic cancer cells and identifying key biologically relevant factors that may account for its aberrant expression in pancreatic cancer.
PMID: 19757157 [Pu...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Zhang J, Zhang X, Zhu Y, Chen Z, Xu Z, Miao Y Tags: Mol Biol Rep Source Type: journals
A PCR-based SNP marker for specific authentication of Korean ginseng (panax ginseng) cultivar "Chunpoong"Email this article to a colleague.
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Korean ginseng (Panax ginseng) has been developed as a horticultural crop due to the increasing demand in the world market. "Chunpoong" is an economically important cultivar with superior quality and high yield among nine cultivars of Korean ginseng. The aim of this work was to develop a simple technique for specific authentication of Chunpoong using DNA method. Molecular authentication of Chunpoong was investigated using DNA sequences of mitochondrial cytochrome oxidase subunit 2 (cox2) intron I and intron II regions. A single nucleotide polymorphism (SNP) specific to Chunpoong was detected and amplification refractor...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Wang H, Sun H, Kwon WS, Jin H, Yang DC Tags: Mol Biol Rep Source Type: journals
PhiC31 integrase interacts with TTRAP and inhibits NFkappaB activation.Email this article to a colleague.
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Phage PhiC31 integrase-mediated gene delivery is believed to be safer than using retroviral vectors since the protein confines its insertion of the target gene to a limited number of sites in mammalian genomes. To evaluate its safety in human cells, it is important to understand the interactions between this integrase and cellular proteins. Here we show that PhiC31 integrase interacts with TTRAP as presented by yeast two-hybrid and co-immunoprecipitation assays. Reducing the expression of endogenous TTRAP can increase the efficiency of PhiC31 integrase-mediated integration. A possible effect of interaction between PhiC...
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Wang BY, Xu GL, Zhou CH, Tian L, Xue JL, Chen JZ, Jia W Tags: Mol Biol Rep Source Type: journals
Expression, purification and characterization of recombinant human interleukin-22 in Pichia pastoris.Email this article to a colleague.
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This study provides a new strategy for large-scale production of bioactive IL-22 for use in basic studies and therapeutic applications.
PMID: 19760099 [PubMed - as supplied by publisher]
Source: Molecular Biology Reports - September 15, 2009 Category: Molecular Biology Authors: Cai X, Wang J, Wang Y, Yang Y, Gao J, Fu W, Wang J, Xu D Tags: Mol Biol Rep Source Type: journals
Filamin a Regulates Caveolae Internalization and Trafficking in Endothelial Cells.Email this article to a colleague.
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Monitoring Editor: Sean Munro Transcytosis via caveolae is critical for maintaining vascular homeostasis by regulating the tissue delivery of macromolecules, hormones and lipids. In the present study, we test the hypothesis interactions between F-actin cross-linking protein filamin A and caveolin-1 facilitates the internalization and trafficking of caveolae. siRNA-mediated knockdown of filamin A, but not filamin B, reduced the uptake and transcytosis of albumin by approximately 35 and 60% respectively, without altering the actin cytoskeletal structure or cell-cell adherens junctions. Mobility of both intracellular cave...
Source: Mol Biol Cell - September 15, 2009 Category: Molecular Biology Authors: Sverdlov M, Shinin V, Place AT, Castellon M, Minshall RD Tags: Mol Biol Cell Source Type: journals
SUN-1 and ZYG-12, Mediators of Centrosome-Nucleus Attachment, Are a Functional SUN/KASH Pair in C. elegans.Email this article to a colleague.
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Monitoring Editor: David G. Drubin KASH/SUN protein pairs can act as connectors between cytoplasmic organelles and the nucleoskeleton. C. elegans ZYG-12 and SUN-1 are essential for centrosome-nucleus attachment. While SUN-1 has a canonical SUN domain, ZYG-12 has a divergent KASH domain. Here we establish that the ZYG-12 mini KASH domain is functional and, in combination with a portion of coiled-coil domain, is sufficient for nuclear envelope localization. ZYG-12 and SUN-1 are hypothesized to be outer and inner nuclear membrane proteins, respectively, and to interact, but neither their topologies nor their physical inte...
Source: Mol Biol Cell - September 15, 2009 Category: Molecular Biology Authors: Minn I, Rolls MM, Hanna-Rose W, Malone CJ Tags: Mol Biol Cell Source Type: journals
Glucose Promotes Stress Resistance in the Fungal Pathogen, Candida albicans.Email this article to a colleague.
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Monitoring Editor: Charles Boone Metabolic adaptation, and in particular the modulation of carbon assimilatory pathways during disease progression, is thought to contribute to the pathogenicity of Candida albicans. Therefore, we have examined the global impact of glucose upon the C. albicans transcriptome, testing the sensitivity of this pathogen to wide-ranging glucose levels (0.01, 0.1 and 1.0%). We show that, like S. cerevisiae, C. albicans is exquisitely sensitive to glucose, regulating central metabolic genes even in response to 0.01% glucose. This indicates that glucose concentrations in the bloodstream (approxim...
Source: Mol Biol Cell - September 15, 2009 Category: Molecular Biology Authors: Rodaki A, Bohovych IM, Enjalbert B, Young T, Odds FC, Gow NA, Brown AJ Tags: Mol Biol Cell Source Type: journals
Expression and Activity of Phosphodiesterase Isoforms during Epithelial Mesenchymal Transition - The Role of PDE4.Email this article to a colleague.
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Monitoring Editor: Keith E. Mostov Epithelial-to-mesenchymal transition (EMT) has emerged as a critical event in the pathogenesis of organ fibrosis and cancer, and is typically induced by the multifunctional cytokine transforming growth factor-beta1 (TGF-beta1). The present study was undertaken to evaluate the potential role of phosphodiesterases (PDEs) in TGF-beta1 induced EMT in the human alveolar epithelial type-II cell line-A549. Stimulation of A549 with TGF-beta1 induced EMT by morphological alterations and by expression changes of the epithelial phenotype markers, E-cadherin, cytokeratin-18, zona occludens-1, and...
Source: Mol Biol Cell - September 15, 2009 Category: Molecular Biology Authors: Kolosionek E, Savai R, Ghofrani HA, Weissmann N, Guenther A, Grimminger F, Seeger W, Banat GA, Schermuly RT, Pullamsetti SS Tags: Mol Biol Cell Source Type: journals
Sox9 Family Members Negatively Regulate Maturation and Calcification of Chondrocytes through Up-Regulation of PTHrP.Email this article to a colleague.
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In this study, we attempted to clarify roles of Sox9 in the late stages of chondrocyte differentiation. We found that overexpression of Sox9 alone or Sox9 together with Sox5 and Sox6 (Sox5/6/9), inhibited the maturation and calcification of murine primary chondrocytes, and up-regulated parathyroid hormone related protein (PTHrP) expression in primary chondrocytes and the mesenchymal cell line C3H10T1/2. Sox5/6/9 stimulated the early stages of chondrocyte proliferation and development. In contrast, Sox5/6/9 inhibited maturation and calcification of chondrocytes in organ culture. The inhibitory effects of Sox5/6/9 were rescu...
Source: Mol Biol Cell - September 15, 2009 Category: Molecular Biology Authors: Amano K, Hata K, Sugita A, Takigawa Y, Ono K, Wakabayashi M, Kogo M, Nishimura R, Yoneda T Tags: Mol Biol Cell Source Type: journals
Rabex-5 Is a Rab22 Effector and Mediates a Rab22-Rab5 Signaling Cascade in Endocytosis.Email this article to a colleague.
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Monitoring Editor: Adam Linstedt Rabex-5 targets to early endosomes and functions as a guanine nucleotide exchange factor (GEF) for Rab5. Membrane targeting is critical for Rabex-5 to activate Rab5 on early endosomes in the cell. Here we report the identification of Rab22 as a binding site on early endosomes for direct recruitment of Rabex-5 and activation of Rab5, establishing a Rab22-Rab5 signaling relay to promote early endosome fusion. Rab22 in GTPgammaS-loaded form, but not GDP-loaded form, binds to the EET domain (residues 81-230) of Rabex-5 in pull-down assays. Rabex-5 targets to Rab22-containing early endosomes...
Source: Mol Biol Cell - September 15, 2009 Category: Molecular Biology Authors: Zhu H, Liang Z, Li G Tags: Mol Biol Cell Source Type: journals
Interaction between Poly(ADP-ribose) and NuMA Contributes to Mitotic Spindle Pole Assembly.Email this article to a colleague.
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Monitoring Editor: Yixian Zheng Poly(ADP-ribose) (pADPr), made by PARP-5a/Tankyrase-1, localizes to the poles of mitotic spindles, and is required for bipolar spindle assembly, but its molecular function in the spindle is poorly understood. To investigate this, we localized pADPr at spindle poles by immuno-EM. We then developed a concentrated mitotic lysate system from HeLa cells to probe spindle pole assembly in vitro. Microtubule asters assembled in response to centrosomes and Ran-GTP in this system. Magnetic beads coated with pADPr, extended from PARP-5a, also triggered aster assembly, suggesting a functional role o...
Source: Mol Biol Cell - September 15, 2009 Category: Molecular Biology Authors: Chang P, Coughlin M, Mitchison TJ Tags: Mol Biol Cell Source Type: journals
Streamlined Architecture and GPI-dependent Trafficking in the Early Secretory Pathway of African Trypanosomes.Email this article to a colleague.
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Monitoring Editor: Benjamin S. Glick The variant surface glycoprotein (VSG) of bloodstream form Trypanosoma brucei is a critical virulence factor. The VSG GPI-anchor strongly influences passage through the early secretory pathway. Using a dominant negative mutation of TbSar1 we show that ER exit of secretory cargo in trypanosomes is dependent on the COPII machinery. Trypanosomes have two orthologues each of the Sec23 and Sec24 COPII subunits, which form specific heterodimeric pairs: TbSec23.1/TbSec24.2 and TbSec23.2/TbSec24.1. RNAi silencing of each subunit is lethal, but has minimal effects on trafficking of soluble a...
Source: Mol Biol Cell - September 15, 2009 Category: Molecular Biology Authors: Sevova ES, Bangs JD Tags: Mol Biol Cell Source Type: journals
Incorporation of excess gadolinium into human bone from medical contrast agentsEmail this article to a colleague.
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Thomas H. Darrah, Jennifer J. Prutsman-Pfeiffer, Robert J. Poreda, M. Ellen Campbell, Peter V. Hauschka, Robyn E. Hannigan
(From Metallomics)
Thomas H. Darrah, Metallomics, 2009, DOI: 10.1039/b905145g
To cite this article before page numbers are assigned, use the DOI form of citation above.
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Source: RSC - Chemical Biology Virtual Journal latest articles - September 15, 2009 Category: Molecular Biology Authors: Thomas H. DarrahJennifer J. Prutsman-Pfeiffer Robert J. Poreda M. Ellen Campbell Peter V. Hauschka Robyn E. Hannigan Source Type: journals
Modulation of bone marrow stromal cell functions in infectious diseases by toll-like receptor ligandsEmail this article to a colleague.
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Abstract Bone marrow-derived stromal cells (BMSCs, or as they are frequently referred to as mesenchymal stem cells) have been long
known to support hematopoiesis and to regenerate bone, cartilage, and adipose tissue. In the last decade, however, a vast
amount of data surfaced in the literature to suggest new roles for these cells including tissue regeneration and immunomodulation.
A great number of review articles appeared that summarize these new data and focus on different aspects of the physiology
of these cells. In this present short review, we will try to summarize the available data based on both mouse...
Source: Journal of Molecular Medicine - September 15, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Therapeutic potential of olfactory ensheathing cells in neurodegenerative diseasesEmail this article to a colleague.
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Abstract The regenerative capacity of the olfactory system has generated interest in potential clinical application of cells from the
olfactory epithelium in the treatment of neurodegenerative diseases. Experimental evidence from animal models and clinical
studies suggest that transplantation of olfactory ensheathing cells (OEC), specialized glia in the olfactory system, may be
therapeutically useful in neurodegenerative diseases such as spinal cord injury and stroke. This review article describes
the different experimental approaches in OEC transplantation. We also discuss the possible effects of OEC implan...
Source: Journal of Molecular Medicine - September 15, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Sgk1 activates MDM2-dependent p53 degradation and affects cell proliferation, survival, and differentiationEmail this article to a colleague.
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Abstract Serum and glucocorticoid regulated kinase 1 (Sgk1) is a serine–threonine kinase that is activated by serum, steroids, insulin,
vasopressin, and interleukin 2 at the transcriptional and post-translational levels. Sgk1 is also important in transduction
of growth factors and steroid-dependent survival signals and may have a role in the development of resistance to cancer chemotherapy.
In the present paper, we demonstrate that Sgk1 activates MDM2-dependent p53 ubiquitylation. The results were obtained in RKO
cells and other cell lines by Sgk1-specific RNA silencing and were corroborated in an original...
Source: Journal of Molecular Medicine - September 15, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
A novel mechanism for inflammation-associated carcinogenesis; an important role of activation-induced cytidine deaminase (AID) in mutation inductionEmail this article to a colleague.
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Abstract Inflammation is a risk for cancer development; however, its mechanism is unknown. Recent studies have revealed that activation-induced
cytidine deaminase (AID), which plays essential roles in both class-switch recombination and somatic hypermutation of immunoglobulin
gene in B lymphocytes, is aberrantly expressed in non-lymphoid cells not only by H.pylori and HCV infection but also by various proinflammatory cytokines, leading to the generation of gene mutations. These findings
not only suggested a new mechanism of inflammation-associated carcinogenesis but has also opened up a new field of tumor bio...
Source: Journal of Molecular Medicine - September 15, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Translational research of novel hormones: lessons from animal models and rare human diseases for common human diseasesEmail this article to a colleague.
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Abstract Since the 1980s, a number of bioactive molecules, now known as cardiovascular hormones, have been isolated from the heart
and blood vessels, particularly from the subset of vascular endothelial cells. The natriuretic peptide family is the prototype
of the cardiovascular hormones. Over the following decade, a variety of hormones and cytokines, now known as adipokines or
adipocytokines, have also been isolated from adipose tissue. Leptin is the only adipokine demonstrated to cause an obese phenotype
in both animals and humans upon deletion. Thus, the past two decades have seen the identification of tw...
Source: Journal of Molecular Medicine - September 15, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Activation of Akt/GSK-3β signaling pathway is involved in intermedin1–53 protection against myocardial apoptosis induced by ischemia/reperfusionEmail this article to a colleague.
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In conclusion, IMD1–53 exerts cardioprotective effect against myocardial I/R injury through the activation of the Akt/GSK-3β signaling pathway to
inhibit mitochondria-mediated myocardial apoptosis.
Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10495-009-0398-7Authors
Jun-Qiu Song, Peking University Health Science Center Department of Physiology and Pathophysiology 100191 Beijing ChinaXu Teng, Peking University Health Science Center Department of Physiology and Pathophysiology 100191 Beijing ChinaYan Cai, Peking University Health Science Center Department of Physiology and Pathophysiology 100191 B...
Source: Apoptosis - September 15, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Serum deprivation induced autophagy and predominantly an AIF-dependent apoptosis in hippocampal HT22 neuronsEmail this article to a colleague.
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Abstract Neuronal death induced by serum deprivation (SD) in HT22-cells was accompanied by a moderate activation of caspase-3, a prominent
upregulation of AIF and its translocation into the nucleus. In addition protein levels of autophagy markers such as LC3 and
beclin-1 were affected by SD. The ratio of LC3-II/LC3-I was significantly increased in serum deprived cultures. Furthermore,
the addition of the pan-caspase inhibitor z-VAD(OMe)-FMK (zVAD) does not protect HT22-cells from SD-induced neurodegeneration.
However, addition of the autophagy inhibitors such as 3-methyladenine (3-MA) or bafilomycin A1 (BafA...
Source: Apoptosis - September 15, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Conjugation of doxorubicin to cell penetrating peptides sensitizes human breast MDA-MB 231 cancer cells to endogenous TRAIL-induced apoptosisEmail this article to a colleague.
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In this study,
we demonstrate that both Dox and Dox–CPPs can increase the density of the TRAIL receptors DR4 and DR5 at the plasma membrane
and moderately sensitize MDA-MB 231 cells to exogeneously added recombinant TRAIL, as has already been shown for other chemotherapeutic
drugs. Moreover, we show that Dox–CPPs, used alone, induce the clustering of TRAIL receptors into ceramide-enriched membrane
lipid rafts, a property not shared by unconjugated Dox and that this process is due to the generation of ceramide during Dox–CPPs
treatment. In addition, MDA-MB 231 cells were found to express TRAIL and we show that th...
Source: Apoptosis - September 15, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Up-regulation of GADD45α expression by NSAIDs leads to apoptotic and necrotic colon cancer cell deathsEmail this article to a colleague.
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In this study, we report that indomethacin and sulindac sulfide
treatments up-regulate GADD45α mRNA expression and protein levels in colon cancer HT-29, RKO and Caco-2 cells. This up-regulation
of GADD45α is accompanied by necrotic cell death and apoptosis. Anti-sense suppression of GADD45α expression inhibited indomethacin
and sulindac sulfide-induced necrotic cell death and apoptosis. These findings confirm a role for GADD45α in NSAID-induced
cytotoxicity, a mechanism for the anti-neoplastic effect of NSAIDs in colon tumorigenesis and cancer growth.
Content Type Journal ArticleCategory Original PaperDOI 10.100...
Source: Apoptosis - September 15, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Endoplasmic reticulum stress-induced apoptosis in the development of diabetes: is there a role for adipose tissue and liver?Email this article to a colleague.
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Abstract Diabetes mellitus (DM) is a multifactorial chronic metabolic disease characterized by hyperglycaemia. Several different mechanisms
have been implicated in the development of the disease, including endoplasmic reticulum (ER) stress. ER stress is increasingly
acknowledged as an important mechanism in the development of DM, not only for β-cell loss but also for insulin resistance.
Accumulating evidence suggests that ER stress-induced apoptosis may be an important mode of β-cell loss and therefore important
in the development of diabetes. Recent data also suggest a role of ER stress-induced apoptosis ...
Source: Apoptosis - September 15, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Predicting Early Chemotherapy Response with Technetium-99m Methoxyisobutylisonitrile SPECT/CT in Advanced Non-Small Cell Lung CancerEmail this article to a colleague.
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Conclusions Our series demonstrated solid, negative correlations between prechemotherapy 99mTc-MIBI uptake and tumor size change measured by CT for advanced NSCLC, particularly with C
mean and 1D change.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0250-zAuthors
Tzu-I J. Yang, The Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital Department of Radiotherapy Plesmanlaan 121 1066 CX Amsterdam The NetherlandsTjeerd S. Aukema, The Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital Department of Nuclear Medicine Amsterdam The NetherlandsHarm van Tintere...
Source: Molecular Imaging and Biology - September 15, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
Endometriosis: science and senseEmail this article to a colleague.
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Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Winterhager, E., Fazleabas, A., Hillier, S. Tags: Editorial Source Type: journals
The non-human primate model of endometriosis: research and implications for fecundityEmail this article to a colleague.
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The development of an animal model of endometriosis is crucial for the investigation of disease pathogenesis and therapeutic intervention. These models will enhance our ability to evaluate the causes for the subfertility associated with disease and provide a first-line validation of treatment modulators. Currently rodents and non-human primate models have been developed, but each model has their limitations. The aim of this manuscript is to summarize the current findings and theories on the development of endometriosis and disease progression and the effectiveness of therapeutic targets using the experimental induced model...
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Braundmeier, A.G., Fazleabas, A.T. Tags: New Research Horizon Reviews Source Type: journals
