Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 25.
Conjugation of doxorubicin to cell penetrating peptides sensitizes human breast MDA-MB 231 cancer cells to endogenous TRAIL-induced apoptosis
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In this study,
we demonstrate that both Dox and Dox–CPPs can increase the density of the TRAIL receptors DR4 and DR5 at the plasma membrane
and moderately sensitize MDA-MB 231 cells to exogeneously added recombinant TRAIL, as has already been shown for other chemotherapeutic
drugs. Moreover, we show that Dox–CPPs, used alone, induce the clustering of TRAIL receptors into ceramide-enriched membrane
lipid rafts, a property not shared by unconjugated Dox and that this process is due to the generation of ceramide during Dox–CPPs
treatment. In addition, MDA-MB 231 cells were found to express TRAIL and we show that th...
Source: Apoptosis - September 15, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Up-regulation of GADD45α expression by NSAIDs leads to apoptotic and necrotic colon cancer cell deathsEmail this article to a colleague.
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In this study, we report that indomethacin and sulindac sulfide
treatments up-regulate GADD45α mRNA expression and protein levels in colon cancer HT-29, RKO and Caco-2 cells. This up-regulation
of GADD45α is accompanied by necrotic cell death and apoptosis. Anti-sense suppression of GADD45α expression inhibited indomethacin
and sulindac sulfide-induced necrotic cell death and apoptosis. These findings confirm a role for GADD45α in NSAID-induced
cytotoxicity, a mechanism for the anti-neoplastic effect of NSAIDs in colon tumorigenesis and cancer growth.
Content Type Journal ArticleCategory Original PaperDOI 10.100...
Source: Apoptosis - September 15, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Endoplasmic reticulum stress-induced apoptosis in the development of diabetes: is there a role for adipose tissue and liver?Email this article to a colleague.
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Abstract Diabetes mellitus (DM) is a multifactorial chronic metabolic disease characterized by hyperglycaemia. Several different mechanisms
have been implicated in the development of the disease, including endoplasmic reticulum (ER) stress. ER stress is increasingly
acknowledged as an important mechanism in the development of DM, not only for β-cell loss but also for insulin resistance.
Accumulating evidence suggests that ER stress-induced apoptosis may be an important mode of β-cell loss and therefore important
in the development of diabetes. Recent data also suggest a role of ER stress-induced apoptosis ...
Source: Apoptosis - September 15, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Predicting Early Chemotherapy Response with Technetium-99m Methoxyisobutylisonitrile SPECT/CT in Advanced Non-Small Cell Lung CancerEmail this article to a colleague.
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Conclusions Our series demonstrated solid, negative correlations between prechemotherapy 99mTc-MIBI uptake and tumor size change measured by CT for advanced NSCLC, particularly with C
mean and 1D change.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0250-zAuthors
Tzu-I J. Yang, The Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital Department of Radiotherapy Plesmanlaan 121 1066 CX Amsterdam The NetherlandsTjeerd S. Aukema, The Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital Department of Nuclear Medicine Amsterdam The NetherlandsHarm van Tintere...
Source: Molecular Imaging and Biology - September 15, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
Endometriosis: science and senseEmail this article to a colleague.
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Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Winterhager, E., Fazleabas, A., Hillier, S. Tags: Editorial Source Type: journals
The non-human primate model of endometriosis: research and implications for fecundityEmail this article to a colleague.
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The development of an animal model of endometriosis is crucial for the investigation of disease pathogenesis and therapeutic intervention. These models will enhance our ability to evaluate the causes for the subfertility associated with disease and provide a first-line validation of treatment modulators. Currently rodents and non-human primate models have been developed, but each model has their limitations. The aim of this manuscript is to summarize the current findings and theories on the development of endometriosis and disease progression and the effectiveness of therapeutic targets using the experimental induced model...
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Braundmeier, A.G., Fazleabas, A.T. Tags: New Research Horizon Reviews Source Type: journals
Epigenetics of endometriosisEmail this article to a colleague.
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In conclusion, several lines of evidence suggest that epigenetics plays a definite role in the pathogenesis and pathophysiology of endometriosis. As such, endometriosis is possibly treatable by rectifying epigenetic aberrations through pharmacological means. DNA methylation markers may also be useful for diagnostic and prognostic purposes. It is also possible that the delineation of the epigenetic changes accompanied by the genesis and progression of endometriosis could lead to interventions that reduce the risk of developing endometriosis.
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Guo, S.-W. Tags: New Research Horizon Reviews Source Type: journals
Reassessing the evidence for the link between dioxin and endometriosis: from molecular biology to clinical epidemiologyEmail this article to a colleague.
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In conclusion, it seems that there is insufficient evidence at this moment in support of the hypothesis that dioxin exposure may lead to increased risk of developing endometriosis in women.
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Guo, S.-W., Simsa, P., Kyama, C. M., Mihalyi, A., Fulop, V., Othman, E.-E. R., D'Hooghe, T. M. Tags: New Research Horizon Reviews Source Type: journals
MicroRNA expression profiling of eutopic secretory endometrium in women with versus without endometriosisEmail this article to a colleague.
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We report distinct miRNA expression profiles in the ESE of women with versus without endometriosis in a subset of samples previously used in global gene expression analysis. Specifically, the miR-9 and miR-34 miRNA families evidenced dysregulation. Integration of the miRNA and gene expression profiles provides unique insights into the molecular basis of this enigmatic disorder and, possibly, the regulation of the proliferative phenotype during the early secretory phase of the menstrual cycle in affected women.
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Burney, R.O., Hamilton, A.E., Aghajanova, L., Vo, K.C., Nezhat, C.N., Lessey, B.A., Giudice, L.C. Tags: Articles Source Type: journals
Progestins inhibit expression of MMPs and of angiogenic factors in human ectopic endometrial lesions in a mouse modelEmail this article to a colleague.
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Progestins are successfully used in the treatment of endometriosis; however, the exact mechanisms of their action are still unsolved. We here focused on the effect of different progestins on parameters of extracellular matrix degradation and angiogenesis involved in the establishment and maintenance of ectopic endometrial lesions. Human endometrium was intraperitoneally transplanted into nude mice. After 7 and 28 days of treatment with progesterone, dydrogesterone, or its metabolite dihydrodydrogesterone, respectively, ectopic lesions were evaluated for proliferation and apoptosis. Expression of estrogen receptor , progest...
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Monckedieck, V., Sannecke, C., Husen, B., Kumbartski, M., Kimmig, R., Totsch, M., Winterhager, E., Grummer, R. Tags: Articles Source Type: journals
Connexin expression pattern in the endometrium of baboons is influenced by hormonal changes and the presence of endometriotic lesionsEmail this article to a colleague.
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Experimentally induced endometriosis in baboons serves as an elegant model to discriminate between endometrial genes which are primarily associated with normal endometrial function and those that are changed by the presence of endometriotic lesions. Since connexin genes are characteristic of the hormonally regulated differentiation of the endometrium, we have examined connexin expression in baboon endometrium to delineate if they are altered in response to the presence of endometriotic lesions. Connexin expression in the endometrium of cycling baboons is similar to that of the human endometrium with Connexin(Cx)43 being pr...
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Winterhager, E., Grummer, R., Mavrogianis, P.A., Jones, C.J.P., Hastings, J.M., Fazleabas, A.T. Tags: Articles Source Type: journals
Endometriotic stromal cells lose the ability to regulate cell-survival signaling in endometrial epithelial cells in vitroEmail this article to a colleague.
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In normal endometrium, stromal factors regulate the growth of epithelial cells. However, epithelial cells in endometriotic lesions display increased proliferation and decreased apoptosis. This work tested the hypothesis that in endometriosis stromal cells lose the ability to regulate survival signaling and cell growth in epithelial cells. Primary normal, endometriotic eutopic and ectopic epithelial cells were cultured in the presence of medium conditioned by normal, eutopic and ectopic endometriotic endometrial stromal cells. Endometriotic epithelial cells showed higher Survivin expression than normal epithelial cells. Con...
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Zhang, H., Li, M., Zheng, X., Sun, Y., Wen, Z., Zhao, X. Tags: Articles Source Type: journals
Induction of endometrial epithelial cell invasion and c-fms expression by transforming growth factor betaEmail this article to a colleague.
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Transforming growth factor beta 1 (TGF-β1) levels are increased in the peritoneal fluid of endometriosis patients, and endometrial cells express TGF-β signaling components; however, little is known regarding the role of TGF-β in endometriosis. Our objective was to examine the effects of TGF-β1 on (i) the expression of macrophage colony-stimulating factor receptor encoded by the c-fms gene, (ii) transmesothelial invasiveness of endometrial cells, (iii) cellular proliferation and (iv) attachment to peritoneal mesothelial cells (PMCs). Effects of TGF-β1 on c-fms mRNA expression were determined by real...
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Liu, Y.-g., Tekmal, R. R., Binkley, P. A., Nair, H. B., Schenken, R. S., Kirma, N. B. Tags: Articles Source Type: journals
Differential actions of estrogen and SERMs in regulation of the actin cytoskeleton of endometrial cellsEmail this article to a colleague.
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Estrogen and selective estrogen receptor modulators (SERMs) differentially impact endometrial cell function, however, the biological basis of these differences is not established. Deregulated cell adhesion to the extracellular matrix, cell movement and invasion are related to endometrial disorders, such as endometriosis or endometrial cancer. Remodeling of the actin cytoskeleton is required to achieve cell adhesion and movement. Estrogen receptor (ER) regulates actin and cell membrane remodeling through extra-nuclear signaling cascades. In this article, we show that administration of 17β-estradiol (E2) and tamoxifen (...
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Flamini, M.I., Sanchez, A.M., Goglia, L., Tosi, V., Genazzani, A.R., Simoncini, T. Tags: Articles Source Type: journals
Peroxisome-proliferator activator receptor-gamma activation decreases attachment of endometrial cells to peritoneal mesothelial cells in an in vitro model of the early endometriotic lesionEmail this article to a colleague.
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In conclusion, CTZ significantly decreased EM42 attachment to LP9 cells and HA in an in vitro model of the early endometriotic lesion.
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Kavoussi, S.K., Witz, C.A., Binkley, P.A., Nair, A.S., Lebovic, D.I. Tags: Articles Source Type: journals
Dienogest, a synthetic progestin, inhibits the proliferation of immortalized human endometrial epithelial cells with suppression of cyclin D1 gene expressionEmail this article to a colleague.
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In this study, we examined the direct effects of dienogest on the proliferation of human endometrial epithelial cells using an immortalized cell line. 5-Bromo-2'-deoxyuridine incorporation into the cells was inhibited by dienogest and by progesterone (P4) in dose-dependent fashion at concentrations of 10–8 mol/l or higher. To identify the target genes of dienogest and P4, we screened the expression of 84 genes related to cell cycle regulation by real-time polymerase chain reaction after 6 h of treatment at a concentration of 10–7 mol/l. Results showed that only cyclin D1 expression was significantly down-regula...
Source: Molecular Human Reproduction - September 14, 2009 Category: Molecular Biology Authors: Shimizu, Y., Takeuchi, T., Mita, S., Mizuguchi, K., Kiyono, T., Inoue, M., Kyo, S. Tags: Articles Source Type: journals
Human cell toxicogenomic analysis of bromoacetic acid: A regulated drinking water disinfection by-productEmail this article to a colleague.
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The objective of this research was to integrate in vitro toxicology with focused toxicogenomic analysis of the regulated DBP, bromoacetic acid (BAA) and to evaluate modulation of gene expression involved in DNA damage/repair and toxic responses, with nontransformed human cells. We generated transcriptome profiles for 168 genes with 30 min and 4 hr exposure times that did not induce acute cytotoxicity. Using qRT-PCR gene arrays, the levels of 25 transcripts were modulated to a statistically significant degree in response to a 30 min treatment with BAA (16 transcripts upregulated and nine downregulated). The largest changes ...
Source: Environmental and Molecular Mutagenesis - September 14, 2009 Category: Molecular Biology Authors: Mark G. Muellner, Matias S. Attene-Ramos, Matthew E. Hudson, Elizabeth D. Wagner, Michael J. Plewa Source Type: journals
Differentiation of alloreactive versus CD3/CD28 stimulated T-lymphocytes using Raman spectroscopy: A greater specificity for noninvasive acute renal allograft rejection detectionEmail this article to a colleague.
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In this study we explore its specificity. Seventy-five inactivated, 40 alloantigen-activated, and 75 CD3/CD28-activated T cells were analyzed using RS. CD3/CD28-activated peak magnitudes (PM) were 4.3% to 23.9% lower than inactivated PM at positions: 903, 1031, 1069, 1093, 1155, 1326, and 1449 cm-1, with a difference in peak ratio (PR) observed at the 1182:1195 cm-1 position (0.91 ± 0.06 vs. 1.2 ± 0.01, respectively: P = 0.006). Differences in CD3/CD28- and alloantigen-activated PM were observed at: 903, 1031, 1093, 1155, 1326, and 1449 cm-1, with no PR differences at the 1182:1195 cm-1 position (0.91 ± 0.06 vs. 0.86 ±...
Source: Cytometry Part A - September 14, 2009 Category: Molecular Biology Authors: Kristian L. Brown, Olena Y. Palyvoda, Jagdish S. Thakur, Sandra L. Nehlsen-Cannarella, Omar R. Fagoaga, Scott A. Gruber, Gregory W. Auner Source Type: journals
Cationic rhodium(I)/bisphosphine complex-catalyzed cyclization of 1,6-diynes with carboxylic acidsEmail this article to a colleague.
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Ken Tanaka, Shunsuke Saitoh, Hiromi Hara, Yu Shibata
(Communication from Org. Biomol. Chem.)
Ken Tanaka, Org. Biomol. Chem., 2009, DOI: 10.1039/b913344e
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Source: RSC - Organic Biomolecular Chemistry - September 14, 2009 Category: Molecular Biology Authors: Ken TanakaShunsuke Saitoh Hiromi Hara Yu Shibata Source Type: journals
Inferring speciation modes in a clade of Iberian chafers from rates of morphological evolution in different character systemsEmail this article to a colleague.
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Conclusion:
Structural morphological characters, often used for species recognition, have evolved at a rate proportional to that of the mtDNA, with no evidence of selection according to our measures. The change in body morphospace seem to have evolved neutrally at short term, but the overall change is different from that expected under a pure random drift, reflecting either selection or developmental constraints. Changes in paramere shape were not neutral at short term, possibly reflecting sexual selection, but their overall amount of change over longer evolutionary periods was neutral, possibly reflecting their lack of fu...
Source: BMC Evolutionary Biology - Latest articles - September 14, 2009 Category: Molecular Biology Authors: Dirk AhrensIgnacio Ribera Source Type: journals
Conserved repertoire of orthologous vomeronasal type 1 receptor genes in ruminant species.Email this article to a colleague.
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Conclusions:
Our results suggest that, compared with rodents, the repertoire of orthologous V1R genes is remarkably conserved among the ruminants cow, sheep and goat. We predict that these orthologous V1Rs can detect the same or closely related chemical compound(s) within each orthologous set/pair. Furthermore, all identified goat V1Rs are expressed in the vomeronasal organ and the main olfactory epithelium, suggesting that V1R-mediated ligand information can be detected and processed by both the main and accessory olfactory systems. The fact that ruminant and rodent V1Rs have distinct features suggests that ruminant and r...
Source: BMC Evolutionary Biology - Latest articles - September 14, 2009 Category: Molecular Biology Authors: Hiromi OharaMasato NikaidoAtsuko Date-ItoKazutaka MogiHiroaki OkamuraNorihiro OkadaYukari TakeuchiYuji MoriKimiko Hagino-Yamagishi Source Type: journals
Prevalence and Significance of Coagulation Abnormalities in Community-acquired Pneumonia.Email this article to a colleague.
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In conclusion, coagulation abnormalities were common and persistent in CAP, even among the least ill. These findings underscore the complexity of the coagulation response to infection and offer potential insights into coagulation-based therapeutics in clinical sepsis trials.
PMID: 19753144 [PubMed - as supplied by publisher]
Source: Molecular Medicine - September 14, 2009 Category: Molecular Biology Authors: Milbrandt EB, Reade MC, Lee M, Shook SL, Angus DC, Kong L, Carter M, Yealy DM, Kellum JA Tags: Mol Med Source Type: journals
A systems biology framework for modeling metabolic enzyme inhibition of Mycobacterium tuberculosisEmail this article to a colleague.
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Conclusions:
The predicted results quantitatively reproduced the experimentally measured dose-response curves, ranging over three orders of magnitude in inhibitor concentration. Thus, by allowing for detailed specifications of the underlying enzymatic kinetics, metabolic reactions/constraints, and growth media, our model captured the essential chemical and biological factors that determine the effects of drug inhibition on in vitro growth of M. tuberculosis cells.
Source: BMC Systems Biology - Latest articles - September 14, 2009 Category: Molecular Biology Authors: Xin FangAnders WallqvistJaques Reifman Source Type: journals
BioEssays 10/2009Email this article to a colleague.
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Source: BioEssays - September 14, 2009 Category: Molecular Biology Tags: Contents Source Type: journals
Highlights from this issueEmail this article to a colleague.
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Source: BioEssays - September 14, 2009 Category: Molecular Biology Tags: Highlights Source Type: journals
What we don't know can, and will, hurt usEmail this article to a colleague.
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Source: BioEssays - September 14, 2009 Category: Molecular Biology Authors: Andrew Moore Tags: Editorial Source Type: journals
Humans as second orangutans: sense or nonsense?Email this article to a colleague.
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Source: BioEssays - September 14, 2009 Category: Molecular Biology Tags: Commentary Source Type: journals
Coming next month:Email this article to a colleague.
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Source: BioEssays - September 14, 2009 Category: Molecular Biology Tags: Forthcoming Articles Source Type: journals
Defining elastic fiber interactions by molecular fishing: an affinity purification and mass spectrometry approach.Email this article to a colleague.
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We describe an affinity purification and mass spectrometry strategy that has provided new insights into the molecular interactions of elastic fibers, essential extracellular assemblies that provide elastic recoil in dynamic tissues. Using cell culture models, we have defined primary and secondary elastic fiber interaction networks by identifying molecular interactions with the elastic fiber molecules, fibrillin-1, MAGP-1, fibulin-5 and lysyl oxidase. The sensitivity and validity of our method was confirmed by identification of known interactions with the bait proteins. Our study has revealed novel extracellular protein int...
Source: Molecular and Cellular Proteomics : MCP - September 14, 2009 Category: Molecular Biology Authors: Cain SA, McGovern A, Small E, Ward LJ, Baldock C, Shuttleworth A, Kielty CM Tags: Mol Cell Proteomics Source Type: journals
Molecular cloning and expression characterization of translationally controlled tumor protein in silkworm pupae.Email this article to a colleague.
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A Bombyx mori (B. mori) cDNA was isolated from silkworm pupae cDNA library encoding a homologue of translationally controlled tumor protein (BmTCTPk). BmTCTPk was expressed in E. coli; SDS-PAGE and Western blot showed the molecular weight of recombinant and native BmTCTPk is approximately 28 and 25 kDa, respectively; they are larger than the theoretical molecular weight. Immunohistochemical studies showed that BmTCTPk is uniformly distributed throughout the cytoplasm of BmN cells. In silkworm pupae, BmTCTPk is expressed in the midgut wall, the midgut cavity, and some fat body tissues lying between the midgut wall and b...
Source: Molecular Biology Reports - September 14, 2009 Category: Molecular Biology Authors: Nie Z, Lv Z, Qian J, Chen J, Li S, Sheng Q, Wang D, Shen H, Yu W, Wu X, Zhang Y Tags: Mol Biol Rep Source Type: journals
Effects of cyclic strain on rat tail tenocytes.Email this article to a colleague.
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Cyclical mechanical strain is considered an important component in flexor tendon cell activation to prevent adhesions and enhance the healing process after tissue injury or surgery, but the biochemical events associated with this remain unclear. To address this, we examined the effects of cyclic tension on the expression of hyaluronic acid, an important lubricant and signal transducer in tendon, on its receptor (CD44), and on total glycosaminoglycan content in rat tail derived tendon fibroblasts in vitro. Tenocytes were plated on fibronectin coated silastic membranes and the cultures were held static or subjected to va...
Source: Molecular Biology Reports - September 14, 2009 Category: Molecular Biology Authors: Crockett RJ, Centrella M, McCarthy TL, Grant Thomson J Tags: Mol Biol Rep Source Type: journals
cDNA sequence and tissues expression analysis of lipoprotein lipase from common carp (Cyprinus carpio Var. Jian).Email this article to a colleague.
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A full-length cDNA coding lipoprotein lipase (LPL) was cloned from liver of adult common carp (Cyprinus carpio Var. Jian) by RT-PCR and rapid amplification of cDNA ends (RACE) approaches. The cDNA obtained was 2,411 bp long with a 1,524 bp open reading frame (ORF) encoding 507 amino acids. This amino acid sequence contains two structural regions: N-terminus (24-354 residues) and C-terminus (355-507 residues). Before N-terminus, 1-23 residues is signal peptide, 6-23 residues is transmembrance helix. At N-terminus, some conversed functional sites were found, including two N-linked glycosylation sites Asn(41) and Asn(88);...
Source: Molecular Biology Reports - September 14, 2009 Category: Molecular Biology Authors: Cheng HL, Sun SP, Peng YX, Shi XY, Shen X, Meng XP, Dong ZG Tags: Mol Biol Rep Source Type: journals
The influence of ADAR1's regulation on lymphocyte cell function during rejection.Email this article to a colleague.
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The RNA editing adenosine deaminase gene (ADAR1) expression is wide-spread in lymphocytes. We explore the mechanism of ADAR1 regulation on the function of T primary lymphocytes when rejection occurs by knock-down the expression of ADAR1 in mouse T primary lymphocytes in mixed lymphocyte cultures. The changes of cell proliferation, the expression of ADAR1 and the cell cycle related genes cyclin D1 and A1, cell cycle and apoptosis analysis and mouse gene expression profiles was evaluated. We found that treatment with ADAR1-specific siRNA inhibited allogenic antigen stimulated T cell proliferation, arrested T cell cycle a...
Source: Molecular Biology Reports - September 14, 2009 Category: Molecular Biology Authors: Cai L, Li Y, Liu F, Zhang W, Huo B, Zheng W, Ding R, Guo J, Zhao Q, Dou K Tags: Mol Biol Rep Source Type: journals
Characterization of a novel human HMBOX1 splicing variant lacking the homeodomain and with attenuated transcription repressor activity.Email this article to a colleague.
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We previously identified the human HMBOX1 (homeobox containing 1) gene, which represents a distinct group of HNF transcription factors, and is a potential transcription repressor with highly expression in pancreas. In our present work, we found that there exists a splicing variant of HMBOX1, designated HMBOX1b. Compared to HMBOX1, HMBOX1b encodes a 304 amino acids protein that shares the N-terminal region but has no homeodomain and the C-terminal region because of an alternative exon 7 which results in reading frame shifting. Unlike the highly pancreatic expression of HMBOX1, HMBOX1b was ubiquitous expressed in all hum...
Source: Molecular Biology Reports - September 14, 2009 Category: Molecular Biology Authors: Zhang M, Chen S, Li Q, Ling Y, Zhang J, Yu L Tags: Mol Biol Rep Source Type: journals
Regulation of GSK-3 beta in the proliferation and apoptosis of human thyrocytes investigated using a GSK-3 beta-targeting RNAi adenovirus expression vector: involvement the Wnt/beta-catenin pathway.Email this article to a colleague.
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Disorders in the proliferation and apoptosis of thyrocytes may induce goitre, adenoma and carcinoma in the thyroid. The Wnt/beta-catenin pathway has been demonstrated to be involved in the regulation of cell proliferation, differentiation and apoptosis in various cell lines. The regulatory mechanism on the proliferation and differentiation of thyrocytes is not well characterized. In the present study, a GSK-3beta-targeting RNA interference (RNAi) adenovirus vector was constructed and delivered to primary human thyrocytes. Results showed that the expression of beta-catenin protein in primary human thyrocytes was increas...
Source: Molecular Biology Reports - September 14, 2009 Category: Molecular Biology Authors: Chen G, Jiang Q, You Z, Yao J, Mou L, Lin X, Shen X, You T, Lin Q, Wen J, Lin L Tags: Mol Biol Rep Source Type: journals
Cloning and characterization of a wheat beta-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici.Email this article to a colleague.
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beta-1,3-Glucanases are a group of pathogenesis-related proteins that have been reported to be involved in plant defense against pathogens in many other plant-pathogen systems. However, it was not clear if these genes play similar role in wheat (Triticum aestivum L.) against Puccinia striiformis f. sp. tritici (Pst), the stripe rust pathogen. To investigate the role of beta-1,3-glucanase (EC 3.2.1.39) in the resistance response of wheat (cv. Suwon 11) to stripe rust, a wheat beta-1,3-glucanase gene induced by Pst, designated as TaGlu, was cloned and characterized. TaGlu was predicted to encode a basic protein of 334 am...
Source: Molecular Biology Reports - September 14, 2009 Category: Molecular Biology Authors: Liu B, Xue X, Cui S, Zhang X, Han Q, Zhu L, Liang X, Wang X, Huang L, Chen X, Kang Z Tags: Mol Biol Rep Source Type: journals
Protective effects of remifentanil on septic mice.Email this article to a colleague.
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This study is to explore the effect of remifentanil on inducible nitric oxide synthase (iNOS) expression, IL-6 and IL-10 levels, myeloperoxidase (MPO) activity, white blood cell count in bronchoalveolar (BALF), ALT and AST activity in septic mice. Forty male KM mice were randomly divided into four groups, sham group, cecal ligation and puncture group (CLP group), remifentanil treatment group (R1 group), and remifentanil control group (R2 group). The mouse model of CLP was used to observe ALT and AST activity, white blood cell count in BALF and myeloperoxidase (MPO). IL-6 and IL-10 in lung and liver tissue were assayed by e...
Source: Molecular Biology Reports - September 14, 2009 Category: Molecular Biology Authors: Zongze Z, Jia Z, Chang C, Kai C, Yanlin W Tags: Mol Biol Rep Source Type: journals
Adult human bone marrow stromal cells regulate expression of their MMPs and TIMPs in differentiation type-specific manner.Email this article to a colleague.
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Previously, we described a profound impact of structural conformation of collagen matrix on osteogenic and adipogenic differentiation of bone marrow stromal cells. Thus, a marginal p38-independent adipogenesis on native collagen I matrix contrasts with an efficient p38-dependent differentiation on denatured collagen I. An efficient Hsp90-dependent osteogenesis occurs on native collagen I matrix but not on its denatured counterpart where it is insignificant and proceeds in an Hsp90-independent manner. Whereas only marginal osteogenesis and no detectable adipogenesis of bone marrow stromal cells occur on native collagen ...
Source: Matrix Biology - September 14, 2009 Category: Molecular Biology Authors: Mauney J, Volloch V Tags: Matrix Biol Source Type: journals
Selective oxidative demethylation of veratric acid to vanillic acid by CYP199A4 from Rhodopseudomonas palustris HaA2Email this article to a colleague.
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Stephen G. Bell, Adrian B. H. Tan, Eachan O. D. Johnson, Luet-Lok Wong
(Paper from Mol. BioSyst.)
Stephen G. Bell, Mol. BioSyst., 2009, DOI: 10.1039/b913487e
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Source: RSC - Mol. BioSyst. latest articles - September 13, 2009 Category: Molecular Biology Authors: Stephen G. BellAdrian B. H. Tan Eachan O. D. Johnson Luet-Lok Wong Source Type: journals
A global view of Escherichia coli Rsd protein and its interactionsEmail this article to a colleague.
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Sarah E. Piper, Jennie E. Mitchell, David J. Lee, Stephen J. W. Busby
(Paper from Mol. BioSyst.)
Sarah E. Piper, Mol. BioSyst., 2009, DOI: 10.1039/b904955j
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Source: RSC - Mol. BioSyst. latest articles - September 13, 2009 Category: Molecular Biology Authors: Sarah E. PiperJennie E. Mitchell David J. Lee Stephen J. W. Busby Source Type: journals
Magnesium-mediated intramolecular reductive coupling: a stereoselective synthesis of C2-symmetric 3,4-bis-silyl-substituted adipic acid derivativesEmail this article to a colleague.
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Pintu K. Kundu, Sunil K. Ghosh
(Paper from Org. Biomol. Chem.)
Pintu K. Kundu, Org. Biomol. Chem., 2009, DOI: 10.1039/b910784c
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Source: RSC - Organic Biomolecular Chemistry - September 13, 2009 Category: Molecular Biology Authors: Pintu K. KunduSunil K. Ghosh Source Type: journals
Four-fold click reactions: Generation of tetrahedral methane- and adamantane-based building blocks for higher-order molecular assembliesEmail this article to a colleague.
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Oliver Plietzsch, Christine Inge Schilling, Mariyan Tolev, Martin Nieger, Clemens Richert, Thierry Muller, Stefan Brase
(Paper from Org. Biomol. Chem.)
Oliver Plietzsch, Org. Biomol. Chem., 2009, DOI: 10.1039/b912189g
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Source: RSC - Organic Biomolecular Chemistry - September 13, 2009 Category: Molecular Biology Authors: Oliver PlietzschChristine Inge Schilling Mariyan Tolev Martin Nieger Clemens Richert Thierry Muller Stefan Brase Source Type: journals
The Meyer-Schuster rearrangement for the synthesis of [small alpha],[small beta]-unsaturated carbonyl compoundsEmail this article to a colleague.
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Douglas A. Engel, Gregory B. Dudley
(Perspective from Org. Biomol. Chem.)
Douglas A. Engel, Org. Biomol. Chem., 2009, DOI: 10.1039/b912099h
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Source: RSC - Organic Biomolecular Chemistry - September 13, 2009 Category: Molecular Biology Authors: Douglas A. EngelGregory B. Dudley Source Type: journals
Efficient framework for automated classification of subcellular patterns in budding yeastEmail this article to a colleague.
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Fluorescent-tagging and digital imaging are widely used to determine the subcellular location of proteins. An extensive publicly available collection of images for most proteins expressed in the yeast S. cerevisae has provided both an important source of information on protein location but also a testbed for methods designed to automate the assignment of locations to unknown proteins. The first system for automated classification of subcellular patterns in these yeast images utilized a computationally expensive method for segmentation of images into individual cells and achieved an overall accuracy of 81%. The goal of the ...
Source: Cytometry Part A - September 13, 2009 Category: Molecular Biology Authors: Seungil Huh, Donghun Lee, Robert F. Murphy Source Type: journals
Virtual-core flow cytometryEmail this article to a colleague.
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Traditional flow cytometers use a sheath fluid to position particles or cells for cytometric measurements, but the need for sheath fluid greatly complicates flow cytometric instrumentation. A cytometric detector that is free of the requirements of sheath fluid can simplify the design of flow cytometers and can extend their use into a number of areas. We designed a flow cytometer that uses a combination of three photodetectors to sense the position of a particle in sample stream. The position-sensitive detectors create a virtual core in the sample stream that eliminates the need for sheath fluid. In this article, we demonst...
Source: Cytometry Part A - September 13, 2009 Category: Molecular Biology Authors: Jarred E. Swalwell, Timothy W. Petersen, Ger van den Engh Source Type: journals
Systematic comparative protein expression profiling of clear cell renal cell carcinoma: A pilot study based on the separation of tissue specimen by two-dimensional electrophoresis.Email this article to a colleague.
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Proteome-based technologies represent powerful tools for the analysis of protein expression profiles including the identification of potential cancer candidate biomarkers. Thus, we here provide a comprehensive protein expression map for clear cell renal cell carcinoma established by systematic comparative two-dimensional gel electrophoresis based protein expression profiling of 16 paired tissue systems comprised of ccRCC lesions and corresponding tumor-adjacent renal epithelium using overlapping narrow pH gradients. This approach led to the mapping of 348 distinct spots corresponding to 248 different protein identities...
Source: Molecular and Cellular Proteomics : MCP - September 13, 2009 Category: Molecular Biology Authors: Lichtenfels R, Dressler SP, Zobawa M, Recktenwald CV, Ackermann A, Atkins D, Kersten M, Hesse A, Puttkammer M, Lottspeich F, Seliger B Tags: Mol Cell Proteomics Source Type: journals
Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population.Email this article to a colleague.
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Rare cells not normally present in the peripheral bloodstream, such as circulating tumor cells, have potential applications for development of non-invasive methods for diagnostics or follow up. Obtaining these cells however require some means of discrimination, achievable by cell type specific antibodies. Here we have generated a microselection method allowing antibody selection, by phage display, targeting a single cell in a heterogeneous population. One K562 cell (female origin) was positioned on glass-slide among millions of lymphocytes from male donor, identifying the K562 cell by FISH (XX). Several single cell sel...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Sørensen MD, Agerholm IE, Christensen B, Kølvraa S, Kristensen P Tags: J Cell Mol Med Source Type: journals
Chemical and molecular mechanisms of antioxidants: Experimental approaches and model systems.Email this article to a colleague.
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ABSTRACT Free radicals derived from oxygen, nitrogen and sulfur molecules in the biological system are highly active to react with other molecules due to their unpaired electrons. These radicals are important part of groups of molecules called reactive oxygen/nitrogen species (ROS/RNS), which are produced during cellular metabolism and functional activities and have important roles in cell signaling, apoptosis, gene expression and ion transportation. However, excessive ROS attack bases in nucleic acids, amino acid side chains in proteins and double bonds in unsaturated fatty acids, and cause oxidative stress, which can...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Lü JM, Lin PH, Yao Q, Chen C Tags: J Cell Mol Med Source Type: journals
Acceleration of muscle regeneration by local injection of muscle-specific microRNAs in rat skeletal muscle injury model.Email this article to a colleague.
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Abstract MicroRNA (miRNA)s are a class of non-coding RNAs that regulate gene expression post-transcriptionally. Muscle-specific miRNA, miRNA (miR)-1, miR-133 and miR-206 play a crucial role in the regulation of muscle development and homeostasis. Muscle injuries are a common muscloskeletal disorder, and the most effective treatment has not been established yet. The purpose of this study was to demonstrate that a local injection of double-stranded (ds) miR-1, miR-133 and 206 can accelerate muscle regeneration in a rat skeletal muscle injury model. After the laceration of the rat tibialis anterior muscle, ds miR-1, 133 a...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Nakasa T, Ishikawa M, Shi M, Shibuya H, Adachi N, Ochi M Tags: J Cell Mol Med Source Type: journals
Resistin competes with lipopolysaccharide for binding to Toll-like receptor 4.Email this article to a colleague.
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Abstract. Toll-like receptors (TLRs) are a family of cellular structures activated by recognition of pathogen associated molecular sequences. The activation of TLRs triggers a variety of intracellular mechanisms aiming to protect the host from the invading microorganisms. Lipopolysaccharide (LPS) is the main ligand for TLR4. Here we show that resistin, a cystein-rich protein believed to regulate carbohydrate metabolism, competes with LPS for binding to TLR4. Binding of recombinant resistin to human myeloid and epithelial cells was assessed by flow cytometry and its co-precipitation with TLR4 was demonstrated. Antibodie...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Tarkowski A, Bjersing J, Shestakov A, Bokarewa MI Tags: J Cell Mol Med Source Type: journals
