Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 26.
Role of AKT-Glycogen Synthase Kinase axis in Monocyte Activation in Humans with and without Type 2 Diabetes.
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Monocyte activation by chemokines is a vital trigger for initiation of atherosclerotic process. Circulating levels of Platelet Activating Factor (PAF), a recognized chemokine, is known to be increased in type 2 diabetes that is linked to accelerated atherosclerosis. To explore the molecular basis we examined the signaling pathways involved in PAF induced monocyte activation. PAF increased migration in monocytes obtained from THP-1 cells, nondiabetic and diabetic subjects that was blocked by AKT inhibition. It did so by phosphorylation of GSK-3betaS(9) which was completely blocked by AKT inhibition. Additionally, PAF in...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Nandy D, Asmann YW, Mukhopadhyay D, Basu A Tags: J Cell Mol Med Source Type: journals
Helicobacter pylori urease activates blood platelets through a lipoxygenase-mediated pathway.Email this article to a colleague.
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Abstract The bacterium Helicobacter pylori causes peptic ulcers and gastric cancer in humans by mechanisms yet not fully understood. H. pylori produces urease which neutralizes the acidic medium permitting its survival in the stomach. We have previously shown that ureases from jackbean, soybean or Bacillus pasteurii induce blood platelet aggregation independently of their enzyme activity by a pathway requiring platelet secretion, activation of calcium channels and lipoxygenase-derived eicosanoids. We investigated whether H. pylori urease displays platelet-activating properties and defined biochemical pathways involved ...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Wassermann GE, Olivera-Severo D, Uberti AF, Carlini CR Tags: J Cell Mol Med Source Type: journals
IMATINIB INHIBITS VASCULAR SMOOTH MUSCLE PROTEOGLYCAN SYNTHESIS AND REDUCES LDL BINDING IN VITRO AND AORTIC LIPID DEPOSITION IN VIVO.Email this article to a colleague.
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ABSTRACT The "response to retention" hypothesis of atherogenesis proposes that proteoglycans bind and retain low density lipoproteins (LDL) in the vessel wall. Platelet derived growth factor (PDGF) is strongly implicated in atherosclerosis and stimulates proteoglycan synthesis. Here we investigated the action of the PDGF receptor inhibitor imatinib on PDGF-mediated proteoglycan biosynthesis in vitro, lipid deposition in the aortic wall in vivo and the carotid artery ex vivo. In human vSMCs, imatinib inhibited PDGF mediated (35)S-SO(4) incorporation into proteoglycans by 31% (p<0.01) and inhibited PDGF-mediated size ...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Ballinger ML, Osman N, Hashimura K, de Haan JB, Jandeleit-Dahm K, Allen T, Tannock LR, Rutledge JC, Little PJ Tags: J Cell Mol Med Source Type: journals
Monocyte Transplantation for Neural and Cardiovascular Ischemia Repair.Email this article to a colleague.
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Abstract Neovascularization is an integral process of inflammatory reactions and subsequent repair cascades in tissue injury. Monocytes/macrophages play a key role in the inflammatory process including angiogenesis as well as the defense mechanisms by exerting microbicidal and immunomodulatory activity. Current studies have demonstrated that recruited monocytes/macrophages aid in regulating angiogenesis in ischemic tissue, tumors, and chronic inflammation. In terms of neovascularization followed by tissue regeneration, monocytes/macrophages should be highly attractive for cell-based therapy compared to any other stem c...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Sanberg PR, Park DH, Kuzmin-Nichols N, Cruz E, Hossne NA, Buffolo E, Willing AE Tags: J Cell Mol Med Source Type: journals
TNF-alpha mediated signal transduction pathway is a major determinant of apoptosis in dilated cardiomyopathy.Email this article to a colleague.
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This study examined the hypothesis that cardiac dysfunction and apoptosis in the cardiomyopathic hamsters were associated with tumor necrosis factor-alpha (TNF-alpha)-mediated signaling pathway involving the activation of some pro-apoptotic proteins and/or deactivation of some antiapoptotic proteins. Echocardiographic assessment of 31 week old hamsters indicated an increase in the internal dimension of the left ventricle as well as decreases in the ejection fraction, fractional shortening, and cardiac output without any evidence of cardiac hypertrophy. Increased level of TNF-alpha and apoptosis in cardiomyopathic hearts we...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Das S, Babick AP, Xu YJ, Takeda N, Rodriguez-Levya D, Dhalla NS Tags: J Cell Mol Med Source Type: journals
Chromosomal instability correlates with poor outcome in patients with myelodysplastic syndromes irrespectively of the cytogenetic risk group.Email this article to a colleague.
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In conclusion, elevated CIN levels may be valuable as an early indicator of poor prognosis in MDS, hence corroborating the concept of CIN as a driving force in tumor progression.
PMID: 19754665 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Heilig CE, Löffler H, Mahlknecht U, Janssen JW, Ho AD, Jauch A, Krämer A Tags: J Cell Mol Med Source Type: journals
Genetic Characterisation of Breast Cancer and Implications for Clinical Management.Email this article to a colleague.
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Abstract Breast cancer is a genetic disease caused by the accumulation of mutations in neoplastic cells. In the last few years, high-throughput microarray-based molecular analysis has provided increasingly more coherent information about the genetic aberrations in breast cancer. New biomarkers and molecular techniques are slowly becoming part of the diagnostic and prognostic armamentarium available for pathologists and oncologists to tailor the therapy for breast cancer patients. In this review we will focus on the contribution of breast cancer somatic genetics to our understanding of breast cancer biology and its impa...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Geyer FC, Lopez-Garcia MA, Lambros MB, Reis-Filho JS Tags: J Cell Mol Med Source Type: journals
Affinity pulldown of gamma-secretase and associated proteins from human and rat brain.Email this article to a colleague.
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Abstract gamma-Secretase is a transmembrane protease complex responsible for the processing of a multitude of type 1 transmembrane proteins, including APP and Notch. A functional complex is dependent on the assembly of four proteins; presenilin (PS), nicastrin, Aph-1 and Pen-2. Little is known about how the substrates are selected by gamma-secretase, but it has been suggested that gamma-secretase associated proteins (GSAPs) could be of importance. For instance, it was recently reported from studies in cell lines that TMP21, a transmembrane protein involved in trafficking, binds to gamma-secretase and regulates the proc...
Source: J Cell Mol Med - September 13, 2009 Category: Molecular Biology Authors: Teranishi Y, Hur JY, Welander H, Frånberg J, Aoki M, Winblad B, Frykman S, Tjernberg LO Tags: J Cell Mol Med Source Type: journals
A novel functional role of iduronate-2-sulfatase in zebrafish early development.Email this article to a colleague.
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Sulfated glycosaminoglycan chains of extracellular matrix and cell membrane-tethered proteoglycans exert specific cellular functions by interacting with a broad spectrum of morphogens and growth factors. In humans, a congenital impaired catabolism of sulfated glycosaminoglycans is associated with severe metabolic disorders. Here, we report on the identification and characterization of a zebrafish iduronate sulfatase orthologue. By knocking down its function with antisense morpholino oligos, we demonstrate that iduronate sulfatase plays a critical role during early vertebrate development and its downregulation may be re...
Source: Matrix Biology - September 13, 2009 Category: Molecular Biology Authors: Moro E, Tomanin R, Friso A, Modena N, Tiso N, Scarpa M, Argenton F Tags: Matrix Biol Source Type: journals
Human bone marrow-derived stromal cells show highly efficient thermal stress-resistant adipogenesis on denatured collagen IV matrix but not on its native counterpart.Email this article to a colleague.
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Collagen IV is the major matrix component associated with differentiating adipocytes in adipose tissues, and the understanding of its contribution in adipogenic differentiation could be important for elucidation of mechanisms and processes driving the obesity. Therefore, in the light of our previous findings of differential effects of structural conformation of collagen I matrix on differentiation of bone marrow stromal cells, we investigated whether similar phenomenon occurs on collagen IV matrix in native and denatured structural states. The results of the present study show that native collagen IV is unsupportive of...
Source: Matrix Biology - September 13, 2009 Category: Molecular Biology Authors: Mauney J, Volloch V Tags: Matrix Biol Source Type: journals
Association of serum amyloid A levels with adipocyte size and serum levels of adipokines: Differences between men and women.Email this article to a colleague.
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In conclusion, serum levels of SAA are strongly correlated with serum levels of inflammatory markers as well as measures of glycemic control. There seems to be large sex differences in these associations suggesting that sex-specific factors need to be considered when analyzing SAA levels in relation to metabolic disease.
PMID: 19758820 [PubMed - as supplied by publisher]
Source: Cytokine - September 13, 2009 Category: Molecular Biology Authors: Sjöholm K, Lundgren M, Olsson M, Eriksson JW Tags: Cytokine Source Type: journals
Beryllium uptake and related biological effects studied in THP-1 differentiated macrophagesEmail this article to a colleague.
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Jian Ding, Lin Lin, Wei Hang, Xiaomei Yan
(From Metallomics)
Jian Ding, Metallomics, 2009, DOI: 10.1039/b913265a
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Source: RSC - Chemical Biology Virtual Journal latest articles - September 13, 2009 Category: Molecular Biology Authors: Jian DingLin Lin Wei Hang Xiaomei Yan Source Type: journals
Stem Cell and Gene Therapeutic Strategies for the Treatment of Multiple Sclerosis.Email this article to a colleague.
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Multiple sclerosis is a disease of the central nervous system that predmoninantly affects young adults. The pathogenic mechanisms are complex, however numerous studies indicate that the disease is initiated by an autoimmune attack on protein targets present in the central nervous system. Given that a dysfunctional immune system perpetuates the pathophysiological mechanisms that characterize this inflammatory disorder, several therapeutic approaches that target immune cells or their secreted mediators have been generated and are currently used clinically. Although these strategies have been partially beneficial to a pro...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Siatskas C, Bernard CC Tags: Curr Mol Med Source Type: journals
The Acid Sphingomyelinase/Ceramide Pathway: Biomedical Significance and Mechanisms of Regulation.Email this article to a colleague.
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One of the most intriguing enzymes of sphingolipid biology is acid sphingomyelinase (ASMase). In a phospholipase C reaction, ASMase catalyzes the cleavage of the phosphocholine head group of sphingomyelin to generate ceramide. Cumulative efforts of various laboratories over the past 40 years have placed ASMase and its product ceramide at the forefront of lipid research. Activation of the ASMase/ceramide pathway is a shared response to an ever growing list of receptor and non receptor mediated forms of cellular stress including: death ligands (TNFalpha, TRAIL, Fas ligand), cytokines (IL-1, IFNgamma), radiation, pathogen...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Zeidan YH, Hannun YA Tags: Curr Mol Med Source Type: journals
Diagnostic Approach to Mitochondrial Disorders: the Need for a Reliable Biomarker.Email this article to a colleague.
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Mitochondrial diseases (MD) are disorders caused by impairment of the mitochondrial electron transport chain (ETC). Phenotypes are polymorphous and may range from pure myopathy to multisystemic disorders. The genetic defect can be located on mitochondrial or nuclear DNA. The ETC is needed for oxidative phosphorylation (which provides the cell with the most efficient energetic outcome in terms of ATP production), and consists of five multimeric protein complexes located in the inner mitochondrial membrane. The ETC also requires cytochrome c and a small electron carrier, coenzyme Q10. One of the pathogenic mechanisms of ...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Mancuso M, Orsucci D, Coppedè F, Nesti C, Choub A, Siciliano G Tags: Curr Mol Med Source Type: journals
Ribosome Inactivating Proteins (RIPs) from Momordica charantia for anti Viral Therapy.Email this article to a colleague.
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This review describes the nature and applications of ribosome inactivating proteins (RIPs) from Momordica charantia (bitter melon). RIPs from the plant kingdom have received much attention in biomedical research because they target conserved host protein synthesis machinery and show specificity towards human and animal cell targets. Recent studies aimed at unravelling the enzymatic activities of the M charantia RIPs provide a structural basis for their activities. It has been reported that RIPs are member of the single chain ribosome inactivating protein (SCRIP) family which act irreversibly on ribosome by removing ade...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Puri M, Kaur I, Kanwar RK, Gupta RC, Chauhan A, Kanwar JR Tags: Curr Mol Med Source Type: journals
Sexual Dimorphism in Autoimmune Disease.Email this article to a colleague.
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We briefly survey the concept of autoimmunity and nominate the range of autoimmune diseases that include multisystemic and organ-specific disorders, and cite prevalences of autoimmune diseases in males and females, in humans and in experimental animals. Most human autoimmune diseases such as systemic lupus erythematosus (SLE), multiple sclerosis (MS) and autoimmune thyroid disease, have an increased incidence and prevalence in females, but a few others such as autoimmune diabetes, the Guillain Barré syndrome (GBS) and psoriasis are increased in males. Animal models of autoimmunity show an equivalent sexual dimorph...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: McCombe PA, Greer JM, Mackay IR Tags: Curr Mol Med Source Type: journals
Multiplexed Fluorescence Imaging of Tumor Biomarkers in Gene Expression and Protein Levels for Personalized and Predictive Medicine.Email this article to a colleague.
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Combining groundbreaking research and developments in cancer biomarkers, nanotechnology and molecular targeted medicine, a new realm of therapy is possible: personalized and predictive medicine. Developing a method to detect the overexpression of several tumor marker genes simultaneously, knowing that a single cell generally expresses more than one altered gene, should have a high predictive value for identifying cancer cells amidst the normal cellular background. Theoretically, a cancer's unique molecular profile can be used to predict its invasive and metastatic potential, its ability to evade immune surveillance, an...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Smith MQ, Staley CA, Kooby DA, Styblo T, Wood WC, Yang L Tags: Curr Mol Med Source Type: journals
Osteopontin: An Effector and an Effect of Tumor Metastasis.Email this article to a colleague.
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Osteopontin (OPN) is a matricellular protein that is produced by multiple tissues in our body and is most abundant in bone. It is also produced by cancer cells and plays a determinative role in the growth, progression and metastasis of cancer. Clinically, OPN has been reported to be upregulated in tumor cells per se; this is also reflected by increased levels of OPN in the circulation. Thus, increased OPN levels the plasma are an effect of tumor growth and progression. Functionally, high OPN levels are determinative of higher incidence of bone metastases in mouse models and are clinically correlated with metastatic bon...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Shevde LA, Das S, Clark DW, Samant RS Tags: Curr Mol Med Source Type: journals
Medulloblastoma: Role of Developmental Pathways, DNA Repair Signaling, and Other Players.Email this article to a colleague.
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Medulloblastoma is a cerebellar tumor affecting children and young adults, and accounts for approximately one fifth of all pediatric brain tumors. Despite multimodal therapy that includes surgery, radiotherapy and chemotherapy, recurrence is frequent and overall mortality rate remains relatively high. Moreover, radiation therapy results in severe effects on intellect, and younger age of treatment correlates with larger deficits. Improvements in therapy of this childhood tumor will focus increasingly on the clarification of the exact cellular origin and the genetic mechanisms contributing to tumor formation, and on new ...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Saran A Tags: Curr Mol Med Source Type: journals
Overview of the Molecular Bases of Resistance to Chemotherapy in Liver and Gastrointestinal Tumours.Email this article to a colleague.
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Primary malignancies of the liver and the gastrointestinal tract constitute one of the main health problems worldwide. Together, these types of tumour are the first cause of death due to cancer, followed by lung and breast cancer respectively. One important limitation in the treatment of these tumours is that, with a few exceptions, they exhibit marked resistance to currently available drugs. Moreover, most of them develop chemoresistance during treatment. The mechanisms responsible for drug refractoriness in gastrointestinal tumours include a reduction in drug uptake; enhanced drug export; intracellular inactivation o...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Marin JJ, Romero MR, Martinez-Becerra P, Herraez E, Briz O Tags: Curr Mol Med Source Type: journals
Anabolics in Osteoporosis: The Emerging Therapeutic Tool.Email this article to a colleague.
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Anabolic therapy for osteoporosis has become the most desirable therapeutic option for menopausal osteoporosis. The anabolic agents currently in clinical use are reviewed. Teriparatide (recombinant human 1-34 parathyroid hormone) is used to treat women with menopausal osteoporosis and men at high risk for fractures. Despite PTH's clinical use, the mechanism underlying its anabolic action requires greater elucidation. Proteol (strontium ranelate) acts by inhibiting bone resorption and presumably promoting bone formation. Though clinical trials have shown that strontium ranelate reduces the frequency of both vertebral an...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Trivedi R, Mithal A, Chattopadhyay N Tags: Curr Mol Med Source Type: journals
Microaerophilic Respiratory Metabolism of Plasmodium falciparum Mitochondrion as a Drug Target.Email this article to a colleague.
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The Plasmodium falciparum mitochondrion is an organelle that presents structural and physiological characteristics different from mitochondria in other eukaryotes. Moreover, there are substantial differences in the properties of asexual and sexual mitochondria. One of the reasons is the adaptation of the parasite to different environments, in particular the great differences in oxygen tension between the host and the mosquito. In this review, we present a synthesis of the recent data on the ultrastructure, the genome and the physiology of the mitochondrion. We try to clarify the mitochondrial role in the intraerythrocy...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Torrentino-Madamet M, Desplans J, Travaillé C, James Y, Parzy D Tags: Curr Mol Med Source Type: journals
Unravelling the Role of Infectious Agents in the Pathogenesis of Human Autoimmunity: The Hypothesis of the Retroviral Involvement Revisited.Email this article to a colleague.
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The incidence of autoimmune disorders is increasing worldwide. Several theories have been proposed to explain how the breakdown in the balance between autoregulatory immune pathways and pathogenic autoreactivity generate autoimmunity. On the basis of a large body of epidemiological, clinical and experimental evidence, it has been suggested that an unfortunate interplay of genetic susceptibility and environmental factors must play an important role in generating the abnormal autoimmune response. Although genetic factors have been well dissected, the environmental agents, that may be causative of disease are still under ...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Fierabracci A Tags: Curr Mol Med Source Type: journals
Regulatory Roles of the Ubiquitin-Proteasome System in Cardiomyocyte Apoptosis.Email this article to a colleague.
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Cardiovascular disease is the leading cause of death in the western world. The major contributor of all cardiovascular deaths is myocardial infarction, which often progresses into end-stage heart failure. The loss of cardiomyocytes is a key problem in the development of cardiovascular disease. Two main processes mediate cardiomyocyte loss: necrosis and apoptosis. In contrast to necrosis, apoptosis is a well regulated process essential in normal development and tissue homeostasis. Tight regulation of this process is crucial, especially in post mitotic cells lacking regenerative capacity, like cardiomyocytes. The ubiquit...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Sohns W, van Veen TA, van der Heyden MA Tags: Curr Mol Med Source Type: journals
Perspectives on Tissue Interactions in Development and Disease.Email this article to a colleague.
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From the morphogenetic movements of the three germ layers during development to the reactive stromal microenvironment in cancer, tissue interactions are vital to maintaining healthy organ morphologic architecture and function. The stromal compartment is thought to be complicit in tumor progression and, as such, represents an opportune target for disease therapies. However, recent developments in our understanding of the diversity of the stromal compartment and the lack of appropriate models to study its relevance in human disease have limited our further understanding of the role of tissue interactions in tumor progres...
Source: Current Molecular Medicine - September 12, 2009 Category: Molecular Biology Authors: Strand DW, Franco OE, Basanta D, Anderson AR, Hayward SW Tags: Curr Mol Med Source Type: journals
IDEAL-Q: An automated tool for label-free quantitation analysis using an efficient peptide alignment approach and spectral data validation.Email this article to a colleague.
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In this paper, we present a fully automated tool, called IDEAL-Q, for label-free quantitation analysis. It accepts raw data in the standard mzXML format as well as search results from major search engines, including Mascot, SEQUEST, and X!Tandem, as input data. To quantify as many identified peptides as possible, IDEAL-Q uses an efficient algorithm to predict the elution time of a peptide unidentified in a specific LC-MS/MS run, but identified in other runs. Then, the predicted elution time is used to detect peak clusters of the assigned peptide. Detected peptide peaks are processed by statistical and computational met...
Source: Molecular and Cellular Proteomics : MCP - September 12, 2009 Category: Molecular Biology Authors: Tsou CC, Tasi CF, Tsui YH, Sudhir PR, Wang YT, Chen YJ, Chen JY, Sung TY, Hsu WL Tags: Mol Cell Proteomics Source Type: journals
Differential expression of TRPC channels in the left ventricle of spontaneously hypertensive rats.Email this article to a colleague.
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This study was designed to identify the differential expression of the canonical transient receptor potential (TRPC) channels in the left ventricle of spontaneously hypertensive rats (SHR). Echocardiography studies were performed to compare the left ventricular function in SHR vs. Wistar-Kyoto rats (WKY), and the mRNA level of the TRPC channels was determined by quantitative real-time RT-PCR (qRT-PCR). Western blots were performed to examine whether the mRNA expression corresponded with the protein expression. Compared with the WKY, the mRNA expression of TRPC4 and TRPC5 was significantly increased in the 10-week-old SHR (...
Source: Molecular Biology Reports - September 12, 2009 Category: Molecular Biology Authors: Liu FF, Ma ZY, Li DL, Feng JB, Zhang K, Wang R, Zhang W, Li L, Zhang Y Tags: Mol Biol Rep Source Type: journals
Association of interleukin 1beta gene (+3953) polymorphism and severity of endometriosis in Turkish women.Email this article to a colleague.
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Endometriosis is regarded as a complex trait, in which genetic and environmental factors contribute to the disease phenotype. We investigated whether the interleukin (IL) 1beta (+3953) polymorphism is associated with the severity of endometriosis. Diagnosis of endometriosis was made on the basis of laparoscopic findings. Stage of endometriosis was determined according to the Revised American Fertility Society classification. 118 women were enrolled in the study. 78 women didnot have endometriosis, 6 women had stage I, 3 had stage II, 13 had stage III and 18 had stage IV endometriosis. Polymerase Chain Reaction (PCR), R...
Source: Molecular Biology Reports - September 12, 2009 Category: Molecular Biology Authors: Attar R, Agachan B, Kucukhuseyin O, Toptas B, Attar E, Isbir T Tags: Mol Biol Rep Source Type: journals
The growth-inhibition effect of tamoxifen in the combination chemotherapeutics on the human cholangiocarcinoma cell line QBC939.Email this article to a colleague.
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In the individual application of adriamycin, mitomycin, vindesine and their combined application with tamoxifen for the pre-treatment of the human cholangiocarcinoma cell line QBC939, QBC939 was determined by MTT assay to investigate the inhibitive effect and its initial mechanism of TAM on cell growth. Growth cycle and apoptosis of each group were determined by flow cytometry. Concentration of ADM in QBC939 was detected by flow cytometry. The levels of their P-glycoprotein were detected by immunohistochemistry. The mRNA and protein levels of apoptotic-associated genes Bcl-2 and Bax were determined by western blot and ...
Source: Molecular Biology Reports - September 12, 2009 Category: Molecular Biology Authors: Liu ZH, He YP, Qin H Tags: Mol Biol Rep Source Type: journals
Validation of endogenous reference genes for insecticide-induced and developmental expression profiling of Liposcelis bostsrychophila (Psocoptera: Liposcelididae).Email this article to a colleague.
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Four housekeeping genes named Lbbeta-Actin1, Lbbeta-Actin2, Lbalpha-Tubulin, and LbGapdh were cloned from Liposcelis bostrychophila using the combined techniques of reverse transcriptase-PCR (RT-PCR) with rapid amplification of cDNA ends (RACE). The GenBank accession numbers were FJ196622, FJ447483, FJ595242, and FJ595241, respectively. The full-length cDNA of Lbbeta-Actin1 was a 1,772 bp sequence with an open reading frame (ORF) encoded 376 amino acids, while Lbbeta-Actin2 was 1,350 bp in length containing an ORF encoded 376 amino acids. Furthermore, the 1,565 bp cDNA of Lbalpha-Tubulin had an ORF of 1,350 bp encoding...
Source: Molecular Biology Reports - September 12, 2009 Category: Molecular Biology Authors: Jiang HB, Liu YH, Tang PA, Zhou AW, Wang JJ Tags: Mol Biol Rep Source Type: journals
Molecular characterization, chromosomal location, alternative splicing and polymorphism of porcine GFAT1 gene.Email this article to a colleague.
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Glutamine: fructose-6-phosphate amidotransferase (GFAT) is the rate-limiting enzyme of the hexosamine synthesis pathway, which plays important roles in insulin resistance and glucose toxicity. GFAT1 is one of the two isoenzymes of GFAT. In the present study, we cloned cDNA sequence of the porcine GFAT1 gene and identified a GFAT1 splice variant (designed GFAT1-L) that contains a 54 bp insertion within the coding region. Nested RT-PCR revealed that GFAT1 was ubiquitously expressed in all tested tissues, but GFAT1-L was only expressed in skeletal muscle and heart, not in liver, spleen, lung, kidney, small intestine, stom...
Source: Molecular Biology Reports - September 12, 2009 Category: Molecular Biology Authors: Liu K, Wang G, Zhao SH, Liu B, Huang JN, Bai X, Yu M Tags: Mol Biol Rep Source Type: journals
Role of the Yes and Csk tyrosine kinases in the development of a pathological state in the human retina.Email this article to a colleague.
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Amplification and a cloning of fragments of genes of human retina tyrosine kinases, the nucleotide sequences of which feature a high homology to the gene families of the Yes and Csk tyrosine kinases, and a cloning of the complete coding sequence of the cDNA of the Csk tyrosine kinase gene of the human lymphocytes have been carried out. It has been established that this sequence contains 1,624 bp and encodes a protein that, with a 99% homology, corresponds to the human tyrosine kinase. A comparative analysis of the nucleotide sequences of the full-size cDNA of the Csk tyrosine kinase of the lymphocytes of healthy donors...
Source: Molecular Biology Reports - September 12, 2009 Category: Molecular Biology Authors: Baranova L, Emelyanova V, Volotovski I Tags: Mol Biol Rep Source Type: journals
Identification of a new QTL for Fusarium head blight resistance in the wheat genotype "Wang shui-bai"Email this article to a colleague.
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Fusarium head blight (FHB) is one of the most devastating wheat diseases, causing both yield loss and quality reduction. To detect quantitative trait loci (QTL) responsible for FHB resistance, plants of the F (2:3) population derived from a 'Wangshui-bai' x 'Sy95-7' cross were artificially inoculated. Of 396 simple sequence repeats (SSRs), 125 amplified fragment length polymorphisms were used for FHB resistance QTL analysis. Five QTLs for FHB resistance were detected on chromosomes 3B, 6B, 7A, 1B and 2D. The effect of the QTL located on chromosome 3B on phenotypic variation was 31.69%, while that of the QTL found on 2D...
Source: Molecular Biology Reports - September 12, 2009 Category: Molecular Biology Authors: Zhang M, Zhang R, Yang J, Luo P Tags: Mol Biol Rep Source Type: journals
CD1d gene is a target for a novel amplicon at 1q22-23.1 in human hepatocellular carcinoma.Email this article to a colleague.
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Genome copy number variation (CNV) is one of the mechanisms to regulate the expression level of genes which contributes to the development and progression of cancer. In order to investigate the regions of high-level amplification and potential target genes within these amplicons in hepatocellular carcinoma (HCC), we analyzed HCC cell line (TJ3ZX-01) for CNV regions at the whole genome level using GeneChip Human Mapping 500K array, and also examined the relative copy number and expression levels of the related genes at candidate amplicons in 41 HCC tissues via real-time fluorescence quantitative PCR methods. Through ana...
Source: Molecular Biology Reports - September 12, 2009 Category: Molecular Biology Authors: Zhang SG, Song WQ, Gao YT, Yang B, Du Z Tags: Mol Biol Rep Source Type: journals
Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymesEmail this article to a colleague.
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In this study, recombinant Ste23p and R. norvegicus IDE (RnIDE) were isolated from E. coli, and their enzymatic properties compared. Ste23p was found to cleave established RnIDE substrates, including the amyloid-[beta] peptide (A[beta]1-40) and insulin B-chain. A novel internally quenched fluorogenic substrate (Abz-SEKKDNYIIKGV-nitroY-OH) based on the polypeptide sequence of the yeast P2 a-factor mating propheromone was determined to be a suitable substrate for both Ste23p and RnIDE, and was used to conduct comparative enzymological studies. Both enzymes were most active at 37 °C, in alkaline buffers and in high salt envi...
Source: Yeast - September 11, 2009 Category: Molecular Biology Authors: Benjamin J. Alper, Jarrad W. Rowse, Walter K. Schmidt Source Type: journals
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-beta1.Email this article to a colleague.
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In this study, we report on the application of a real-time noninvasive system for dynamic monitoring of cellular plasticity. Analysis of the cell impedance profile recorded as cell index using a real-time cell analyzer revealed its significant increase after the treatment of prostate epithelial cells with the transforming growth factor-beta1. Changes in the cell index profile were paralleled with cytoskeleton rebuilding and induction of epithelial-mesenchymal transition and negatively correlated with cell proliferation. This novel application of such approach demonstrated a great potential of the impedance-based system for...
Source: Biological Procedures Online - September 11, 2009 Category: Molecular Biology Authors: Staršíchová A, Kubala L, Lincová E, Pernicová Z, Kozubík A, Souček K Tags: Biol Proced Online Source Type: journals
RNA interference: a potent technology in studying and modulating of dendritic cells, and potential in clinical therapy.Email this article to a colleague.
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RNA interference (RNAi) is a natural process by which small interfering RNA duplex directs sequencespecific post-transcriptional silencing of homologous genes by binding to its complementary mRNA and triggering its elimination. The history of RNAi has only about two decades, however, further studies have revealed that it is a potent method of gene silencing that has developed rapidly over the past few years as a result of its extensive importance in the study of genetics, molecular biology and physiology. RNAi technology has also recently yielded significant insight into dendritic cells (DCs) by helping to elucidate nu...
Source: Molecular Biology Reports - September 11, 2009 Category: Molecular Biology Authors: Cheng F, He S Tags: Mol Biol Rep Source Type: journals
Differential display reveals a novel pig gene, PRPF3, which is differentially expressed in Large White versus Wujin skeletal muscle tissues.Email this article to a colleague.
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The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends method. The open reading frame of this gene encodes a protein of 683 amino acids, which is homologous with the PRP3 pre-mRNA processing factor 3 (PRPF3) of five species: bovine (99%), human (99%), sumatran orangutan (99%), mouse (99%) and chicken (94%). This newly identified gene was respective...
Source: Molecular Biology Reports - September 11, 2009 Category: Molecular Biology Authors: Yonggang L Tags: Mol Biol Rep Source Type: journals
Constructing glucagon like peptide-1 receptor fused with derivatives of GFP for visualizing protein-protein interaction in living cells.Email this article to a colleague.
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In this study, YFP-tagged GLP-1 (YFP-GLP-1) receptor and CFP-tagged GLP-1 (CFP-GLP-1) receptor for visualizing protein-protein interaction in living cells were constructed and localized in CHO cells. Cells expressing YFP-GLP-1 and CFP-GLP-1 receptor showed characteristic GLP-1 mediated increase in cAMP, similar to cells expressing a wild type GLP-1 receptor. This means that both types of receptors are functional and localized in plasma membrane.
PMID: 19757164 [PubMed - as supplied by publisher]
Source: Molecular Biology Reports - September 11, 2009 Category: Molecular Biology Authors: Bavec A Tags: Mol Biol Rep Source Type: journals
Molecular cloning and expression analysis of an F-box protein gene responsive to plant hormones in Brassica napus.Email this article to a colleague.
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F-box protein family is characterized by an F-box motif that has been shown to be critical for the controlled degradation of regulatory proteins. In plant, F-box protein plays an important role in signal pathways and involved in various signal transduction systems. A full-length cDNA encoding a putative F-box protein, designated as BnSLY1, was isolated from Brassica napus. The full-length cDNA of BnSLY1 was 809 bp containing a 438 bp open reading frame encoding a precursor protein of 138 amino acid residues. Comparative and bioinformatic analyses revealed that BnSLY1 showed high degree of homology with F-box proteins f...
Source: Molecular Biology Reports - September 11, 2009 Category: Molecular Biology Authors: Gao Y, Zhao Y, Li T, Liu Y, Ren C, Wang M Tags: Mol Biol Rep Source Type: journals
Automated multichannel fluorescent whole slide imaging and its application for cytometryEmail this article to a colleague.
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Slide-based image cytometry (SBC) has several advantages over flow cytometry but it is not widely used because of its low throughput, complicated workflow, and high price. Fully automated microscopes became affordable with the advent of whole slide imaging (WSI) and they can be transformed into a cytometer. A MIRAX MIDI automated whole slide imager was used with metal-halide and light emitting diode (LED)-based fluorescent illumination, filter block changer, and a cooled monochrome charge coupled device camera. The MIRAX control software was further developed for fluorescent sample detection, autofocusing, multichannel dig...
Source: Cytometry Part A - September 10, 2009 Category: Molecular Biology Authors: Viktor Sebestyén Varga, Levente Ficsor, Viktor Kamarás, Viktor Jónás, Tibor Virág, Zsolt Tulassay, Béla Molnár Source Type: journals
Use of flow cytometry as a quality control device for liquid-based cervical cytology specimensEmail this article to a colleague.
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The objective of the study is the evaluation of flow cytometry as a rapid tool for quality control of the liquid specimen adequacy for the purpose of precise cytological diagnosis in detecting cervical abnormalities.A total of 1,854 women undergoing routine cervical cytology joined this cohort study. A monolayer smear was prepared and 1 ml aliquot was removed and prepared for flow cytometric analysis. Cells were fixed and run on a Partec CyFlow SL, with front scatter (FSS) and side scatter (SSC) set on logarithmic scale. The forward scatter versus side scatter dot-plot was used for the distinction of ectocervical, endocerv...
Source: Cytometry Part B: Clinical Cytometry - September 10, 2009 Category: Molecular Biology Authors: Christine Kottaridi, John Georgoulakis, Dimitrios Kassanos, Asimakis Pappas, Aris Spathis, Niki Margari, Dionissios Aninos, Petros Karakitsos Source Type: journals
Exploring synthetic avenues for the effective synthesis of selenium- and tellurium-containing multifunctional redox agentsEmail this article to a colleague.
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Susanne Mecklenburg, Saad Shaaban, Lalla A. Ba, Torsten Burkholz, Thomas Schneider, Britta Diesel, Alexandra K. Kiemer, Anne Roseler, Katja Becker, Jorg Reichrath, Alexandra Stark, Wolfgang Tilgen, Muhammad Abbas, Ludger A. Wessjohann, Florenz Sasse, Claus Jacob
(Paper from Org. Biomol. Chem.)
Susanne Mecklenburg, Org. Biomol. Chem., 2009, DOI: 10.1039/b907831b
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Source: RSC - Organic Biomolecular Chemistry - September 10, 2009 Category: Molecular Biology Authors: Susanne MecklenburgSaad Shaaban Lalla A. Ba Torsten Burkholz Thomas Schneider Britta Diesel Alexandra K. Kiemer Anne Roseler Katja Becker Jorg Reichrath Alexandra Stark Wolfgang Tilgen Muhammad Abbas Ludger A. Wessjohann Florenz Sasse Claus Jacob Source Type: journals
MPR1 as a novel selection marker in Saccharomyces cerevisiaeEmail this article to a colleague.
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L-Azetidine-2-carboxylic acid (AZC) is a toxic four-membered ring analogue of L-proline that is transported into cells by proline transporters. AZC and L-proline in the cells are competitively incorporated into nascent proteins. When AZC is present in a minimum medium, misfolded proteins are synthesized in the cells, thereby inhibiting cell growth. The MPR1 gene has been isolated from the budding yeast Saccharomyces cerevisiae [Sigma]1278b as a multicopy suppressor of AZC-induced growth inhibition. MPR1 encodes a novel acetyltransferase that detoxifies AZC via N-acetylation. Since MPR1 is absent in the laboratory strain of...
Source: Yeast - September 10, 2009 Category: Molecular Biology Authors: Kaoru Ogawa-Mitsuhashi, Koji Sagane, Junro Kuromitsu, Hiroshi Takagi, Kappei Tsukahara Source Type: journals
The presence of CD8(+) invariant NKT cells in mice.Email this article to a colleague.
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Invariant natural killer T (iNKT) cells develop in the thymus upon recognition of CD1d expressed on developing thymocytes. Although CD4 and CD8 coreceptors are not directly involved in the interaction between CD1d and the TCRs of iNKT cells, a conspicuous lack of CD8(+) iNKT cells in mice raised the question of whether CD8(+) iNKT cells are excluded due to negative selection during their thymic development, or if there is no lineage commitment for the development of murine CD8(+) iNKT cells. To address this question, we analyzed iNKT cell-specific TCR Valpha14(+) transgenic mice, where the Valpha14 transgene forces the...
Source: exp Mol Med - September 10, 2009 Category: Molecular Biology Authors: Lee H, Hong C, Shin J, Oh S, Jung S, Park YK, Hong S, Lee GR, Park SH Tags: Exp Mol Med Source Type: journals
Cross-talk between BubR1 expression and commitment to differentiate in adipose-derived mesenchymal stem cells.Email this article to a colleague.
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In this study, we show that BubR1 depletion in human adipose-derived mesenchymal stem cells (ASCs) precedes the loss of differentiation potential and the induction of replicative senescence. These effects occur independently of p16(INK4A) expression and may involve DNA methylation. Our data therefore reveal a new and unsuspected feature of BubR1 expression in the regulation of adult stem cell differentiation.
PMID: 19745606 [PubMed - as supplied by publisher]
Source: exp Mol Med - September 10, 2009 Category: Molecular Biology Authors: Lee J, Lee CG, Lee KW, Lee CW Tags: Exp Mol Med Source Type: journals
Activation of PPARgamma Induces Profound Multilocularization of Adipocytes in Adult Mouse White Adipose Tissues.Email this article to a colleague.
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We sought to determine the effects of activation of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) on multilocularization of adipocytes in adult white adipose tissue (WAT). Male C57BL/6 normal, db/db, and ob/ob mice were treated with agonists of PPAR-gamma, PPAR-alpha, or beta(3)-adrenoceptor for 3 weeks. To distinguish multilocular adipocytes from unilocular adipocytes, whole-mounted adipose tissues were co-immunostained for perilipin and collagen IV. PPAR-gamma activation with rosiglitazone or pioglitazone induced a profound change of unilocular adipocytes into smaller, multilocular adipocytes in adult...
Source: exp Mol Med - September 10, 2009 Category: Molecular Biology Authors: Koh YJ, Park BH, Park JH, Han J, Lee IK, Park JW, Koh GY Tags: Exp Mol Med Source Type: journals
Expression of 4-1BB and 4-1BBL in thymocytes during thymus regeneration.Email this article to a colleague.
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In this study, the expression of 4-1BB and 4-1BBL was examined during regeneration of the murine thymus following acute cyclophosphamide-induced involution. Four-color flow cytometry showed that 4-1BB and 4-1BBL were present in the normal thymus and were preferentially expressed in the regenerating thymus, mainly in CD4(+)CD8(+) double-positive (DP) thymocytes. Furthermore, the CD4(lo)CD8(lo), CD4(+)CD8(lo) and CD4(lo)CD8(+) thymocyte subsets, representing stages of thymocyte differentiation intermediate between DP and single-positive (SP) thymocytes, also expressed 4-1BB and 4-1BBL during thymus regeneration but to a less...
Source: exp Mol Med - September 10, 2009 Category: Molecular Biology Authors: Kim YM, Kim HK, Kim HJ, Lee HW, Ju SA, Choi BK, Kwon BS, Kim BS, Kim JB, Lim YT, Yoon S Tags: Exp Mol Med Source Type: journals
Fibroblasts in Three Dimensional Matrices: Cell Migration and Matrix Remodeling.Email this article to a colleague.
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Fibroblast-collagen matrix culture has facilitated the analysis of cell physiology under conditions that more closely resemble an in vivo-like environment compared to conventional 2-dimensional (2D) cell culture. Furthermore, it has led to significant progress in understanding reciprocal and adaptive interactions between fibroblasts and the collagen matrix, which occur in tissue. Recent studies on fibroblasts in 3-dimensional (3D) collagen matrices have revealed the importance of biomechanical conditions in addition to biochemical cues for cell signaling and migration. Depending on the surrounding mechanical conditions...
Source: exp Mol Med - September 10, 2009 Category: Molecular Biology Authors: Rhee S Tags: Exp Mol Med Source Type: journals
