Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 27.
LIN28B confer radio-resistance through the posttranscriptional control of KRAS.
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To screen the differentially expressed microRNAs related to radio-resistance, we compared the microRNA profiles of lung cancer cells with different responses to ionizing radiation (IR). Of 328 microRNAs in microarray, 27 microRNAs were differentially expressed in NCI-H460 (H460) and NCI-H1299 (H1299) cells. Among them, let-7g was down-regulated in radio-resistant H1299 cells, and the level of let-7g was higher in radio-sensitive cells like Caski, H460, and ME180 in qRT-PCR analysis than in radio-resistant cells like A549, H1299, DLD1, and HeLa. Over-expression of let-7g in H1299 cells could suppress the translation of ...
Source: exp Mol Med - September 10, 2009 Category: Molecular Biology Authors: Jeong SH, Wu HG, Park WY Tags: Exp Mol Med Source Type: journals
Optimization of Streptomyces bacteriophage phiC31 integrase system to prevent post integrative gene silencing in pulmonary type II cells.Email this article to a colleague.
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phiC31 integrase has emerged as a potent tool for achieving long-term gene expression in different tissues. The present study aimed at optimizing elements of phiC31 integrase system for alveolar type II cells. Luciferase and beta galactosidase activities were measured at different time points post transfection. 5-Aza-2'deoxycytidine (AZA) and Trichostatin A (TSA) were used to inhibit DNA methyltransferase and histone deacetylase complex (HDAC) respectively. In A549 cells, expression of the integrase using a CMV promoter resulted in highest integrase activity, whereas in MLE12 cells, both CAG and CMV promoter were equal...
Source: exp Mol Med - September 10, 2009 Category: Molecular Biology Authors: Aneja MK, Geiger J, Imker R, Zg N S, Kormann M, Hasenpusch G, Maucksch C, Rudolph C Tags: Exp Mol Med Source Type: journals
The Roles of Glycosphingolipids in the Proliferation and Neural Differentiation of Mouse Embryonic Stem Cells.Email this article to a colleague.
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In this study, Ugcg expression was reduced to approximately 80% by short hairpin RNAs (shRNAs) to evaluate the roles of GSLs in proliferation and neural differentiation of mouse embryonic stem cells (mESCs). HPTLC/immunofluorescence analyses of shRNA-transfected mESCs revealed that treatment with Ugcg-shRNA decreased expression of major gangliosides, GM3 and GD3. Furthermore, MTT and Western blot/immunofluorescence analyses demonstrated that inhibition of the Ugcg expression in mESCs resulted in decrease of cell proliferation (p <0.05) and decrease of activation of the ERK1/2 (p <0.05), respectively. To further inves...
Source: exp Mol Med - September 10, 2009 Category: Molecular Biology Authors: Jung JU, Lee DH, Ko K, Ko K, Choo YK Tags: Exp Mol Med Source Type: journals
Use of (S)-BINOL as NMR chiral solvating agent for the enantiodiscrimination of omeprazole and its analogs.Email this article to a colleague.
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The application of (S)-1,1'-binaphthyl-2,2'-diol as NMR chiral solvating agent (CSA) for omeprazole, and three of its analogs (lanso-, panto-, and rabe-prazole) was investigated. The formation of diastereomeric host-guest complexes in solution between the CSA and the racemic substrates produced sufficient NMR signal splitting for the determination of enantiomeric excesses by (1)H- or (19)F-NMR spectroscopy. Using of hydrophobic deuterated solvents was mandatory for obtaining good enantiodiscrimination, thus suggesting the importance of intermolecular hydrogen bonds in the stabilization of the complexes. The method was ...
Source: Chirality - September 10, 2009 Category: Molecular Biology Authors: Redondo J, Capdevila A, Latorre I Tags: Chirality Source Type: journals
Theoretical approaches to the calculation of Raman optical activity spectra.Email this article to a colleague.
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In this article, we will give a brief account of the different approaches that have been presented in the literature for calculating Raman optical activity (ROA) spectra by ab initio methods. We will also outline the general structure of a self-consistent-field-based approach for analytic calculations of ROA spectra, including also contributions from London orbitals. The use of London orbitals ensures that the relevant ROA parameters are gauge origin independent. We will also give an outlook on the future of ab initio calculations of Raman optical activity spectra. Chirality 2009. (c) 2009 Wiley-Liss, Inc.
PMID: 19...
Source: Chirality - September 10, 2009 Category: Molecular Biology Authors: Ruud K, Thorvaldsen AJ Tags: Chirality Source Type: journals
Characterization of folded conformations in a tetrapeptide containing two tryptophan residues by vibrational circular dichroism.Email this article to a colleague.
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The intramolecularly hydrogen bonded conformations of the tetrapeptide Boc-Trp-Aib-Gly-Trp-OMe (WUGW) are investigated using experimental and quantum chemical predictions of vibrational circular dichroism (VCD) in the 1800-1550 cm(-1) region. The predicted VCD spectrum, for a conformation (conformer A) obtained from optimization of crystal structure, reproduced the dominant negative VCD band observed experimentally in CH(3)OH and CHCl(3) solvents. However, the predicted VCD spectrum of Conformation A also has an extra positive band which is not seen in the experimental spectra. This mismatch appears to be due to the la...
Source: Chirality - September 10, 2009 Category: Molecular Biology Authors: Petrovic AG, Polavarapu PL, Mahalakshmi R, Balaram P Tags: Chirality Source Type: journals
Circular dichroism spectra, optical rotations and absolute configurations of cis-dihydrodiol metabolites of quinoline and derivatives: The role of the nitrogen atom.Email this article to a colleague.
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The absolute configurations of cis-dihydrodiol bacterial metabolites derived from quinoline substrates and their quinolinium salts have been determined by comparison of experimental and calculated chiroptical data (circular dichroism spectra and optical rotations). The role of intramolecular H-bonding and other interactions in the preferred conformations has been investigated, including the effect of protonation of several cis-dihydrodiols. The presence of a strong intramolecular OH...N bond in several cis-dihydrodiol metabolites suggests that such species have a residually chiral oxygen atom. Chirality 2009. (c) 2009 ...
Source: Chirality - September 10, 2009 Category: Molecular Biology Authors: Kwit M, Gawronski J, Sbircea L, Sharma ND, Kaik M, Boyd DR Tags: Chirality Source Type: journals
Specific processing of tenascin-C by the metalloprotease meprinbeta neutralizes its inhibition of cell spreading.Email this article to a colleague.
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The metalloprotease meprin has been implicated in tissue remodelling due to its capability to degrade extracellular matrix components. Here, we investigated the susceptibility of tenascin-C to cleavage by meprinbeta and the functional properties of its proteolytic fragments. A set of monoclonal antibodies against chicken and human tenascin-C allowed the mapping of proteolytic fragments generated by meprinbeta. In chicken tenascin-C, meprinbeta processed all three major splicing variants by removal of 10kDa N-terminal and 38kDa C-terminal peptides, leaving a large central part of subunits intact. A similar cleavage patt...
Source: Matrix Biology - September 10, 2009 Category: Molecular Biology Authors: Ambort D, Brellier F, Becker-Pauly C, Stöcker W, Andrejevic-Blant S, Chiquet M, Sterchi EE Tags: Matrix Biol Source Type: journals
Hepatic monocyte chemoattractant protein-1 is upregulated by dietary cholesterol and contributes to liver steatosis.Email this article to a colleague.
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To assess the role of monocyte chemoattractant protein-1 (MCP-1/CCL2) in the development of fatty liver, we have used LDLr(-/-) mice as an animal model of high-fat, high-cholesterol diet-induced liver steatosis. The rapid dietary induction of hepatic mRNA MCP-1 expression was paralleled by a concomitant increase in plasma MCP-1 that was strongly associated with the degree of liver steatosis. Hepatocytes showed an intense immunoreactivity for MCP-1 that was mainly located surrounding the hepatic lipid droplets. The intake of cholesterol also increased the concentration of MCP-1 in liver homogenates. This was accompanied...
Source: Cytokine - September 10, 2009 Category: Molecular Biology Authors: Rull A, Rodríguez F, Aragonès G, Marsillach J, Beltrán R, Alonso-Villaverde C, Camps J, Joven J Tags: Cytokine Source Type: journals
Angiotensin II type-1 receptor antagonist attenuates LPS-induced acute lung injury.Email this article to a colleague.
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Angiotensin II is able to trigger inflammatory responses through an angiotensin II type 1 (AT1) receptor. The role of AT1 receptor in acute lung injury (ALI) is poorly understood. Mice were randomly divided into three groups (n=40 each groups): NS group; LPS group (2mg/kg LPS intratracheally); and LPS+ZD 7155 group, 10mg/kg ZD 7155 (an AT1 receptor antagonist) intraperitoneally 30min prior to LPS exposure. Samples from the lung were isolated and assayed for histopathology analyses or proinflammatory gene expressions, angiotensin II receptors expressions and nuclear factors activities. LPS exposure resulted in severe AL...
Source: Cytokine - September 10, 2009 Category: Molecular Biology Authors: Wang F, Xia ZF, Chen XL, Jia YT, Wang YJ, Ma B Tags: Cytokine Source Type: journals
Role of dephosphorylation of FOXO1 on apoptosis induced by wortmannin for non-Hodgkin's lymphoma cells.Email this article to a colleague.
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The aim of this study was to ascertain the relationship between the phosphorylation of FOXO1 and the apoptosis and the proliferation of lymphoma cells so as to further clarify the cellular biology and pathogenesis of the disease. The lymphoma cells Namalwa and Jurkat were treated with PI3K inhibitor wortmannin or etoposide alone or wortmannin plus etoposide with different schedule. The inhibition rates of lymphoma cell growth were examined by XTT assay. Apoptosis were detected by flow cytometry. The expression of p-Akt, p-FOXO1, FOXO1, bim were determined by western blot analysis. Wortmannin induced apoptosis of Jurkat...
Source: Molecular Biology Reports - September 10, 2009 Category: Molecular Biology Authors: Qiong Z, Ruofan H, Xiaohua L, Xinli Z, Jingwei J, Zhaohui C Tags: Mol Biol Rep Source Type: journals
The identification and enumeration of dendritic cell populations from individual mouse spleen and Peyer's patches using flow cytometric analysisEmail this article to a colleague.
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Dendritic cell (DC) research currently involves pooling of tissues from multiple animals followed by enrichment techniques to obtain sufficient numbers of DCs for analysis. Enrichment techniques take advantage of DC adherence, buoyant density properties, and/or positive or negative selection of cell populations using monoclonal antibodies. However, enrichment techniques may significantly change the maturation and/or activation status of DCs or selectively eliminate one or more subpopulations of DCs. To overcome these drawbacks, we designed a multicolor flow cytometric technique for simultaneous analysis of DC populations f...
Source: Cytometry Part A - September 9, 2009 Category: Molecular Biology Authors: David M. Duriancik, Kathleen A. Hoag Source Type: journals
Global analysis of the yeast osmotic stress response by quantitative proteomicsEmail this article to a colleague.
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Boumediene Soufi, Christian D. Kelstrup, Gabriele Stoehr, Florian Frohlich, Tobias C. Walther, Jesper V. Olsen
(Paper from Mol. BioSyst.)
Boumediene Soufi, Mol. BioSyst., 2009, DOI: 10.1039/b902256b
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Source: RSC - Mol. BioSyst. latest articles - September 9, 2009 Category: Molecular Biology Authors: Boumediene SoufiChristian D. Kelstrup Gabriele Stoehr Florian Frohlich Tobias C. Walther Jesper V. Olsen Source Type: journals
DNA supercoiling regulates the stress-inducible expression of genes in the cyanobacterium SynechocystisEmail this article to a colleague.
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Jogadhenu S. S. Prakash, Maria Sinetova, Anna Zorina, Elena Kupriyanova, Iwane Suzuki, Norio Murata, Dmitry A. Los
(Paper from Mol. BioSyst.)
Jogadhenu S. S. Prakash, Mol. BioSyst., 2009, DOI: 10.1039/b903022k
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Source: RSC - Mol. BioSyst. latest articles - September 9, 2009 Category: Molecular Biology Authors: Jogadhenu S. S. PrakashMaria Sinetova Anna Zorina Elena Kupriyanova Iwane Suzuki Norio Murata Dmitry A. Los Source Type: journals
An integrative approach towards completing genome-scale metabolic networksEmail this article to a colleague.
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Nils Christian, Patrick May, Stefan Kempa, Thomas Handorf, Oliver Ebenhoh
(Paper from Mol. BioSyst.)
Nils Christian, Mol. BioSyst., 2009, DOI: 10.1039/b915913b
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Source: RSC - Mol. BioSyst. latest articles - September 9, 2009 Category: Molecular Biology Authors: Nils ChristianPatrick May Stefan Kempa Thomas Handorf Oliver Ebenhoh Source Type: journals
Nuclear receptor complement of the cnidarian Nematostella vectensis: phylogenetic relationships and developmental expression patternsEmail this article to a colleague.
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Conclusion:
N. vectensis contains a diverse complement of nuclear receptors including orthologs of several bilaterian nuclear receptors. Novel nuclear receptors in N. vectensis may be ancient genes lost from triploblastic lineages or may represent cnidarian-specific radiations. Nuclear receptors exhibited distinct developmental expression patterns, which are consistent with diverse regulatory roles for these genes. Understanding the evolutionary relationships and developmental expression of the N. vectensis nuclear receptor complement provides insight into the evolution of the nuclear receptor superfamily and a foundation ...
Source: BMC Evolutionary Biology - Latest articles - September 9, 2009 Category: Molecular Biology Authors: Adam ReitzelAnn Tarrant Source Type: journals
Evolutionary history of selenocysteine incorporation from the perspective of SECIS binding proteinsEmail this article to a colleague.
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Conclusions:
Our analysis suggests that SECIS binding proteins arose once in evolution but diverged significantly in multiple lineages. In addition, likely due to a gene duplication event in the early vertebrate lineage, SBP2 an SBP2L are paralogous in vertebrates.
Source: BMC Evolutionary Biology - Latest articles - September 9, 2009 Category: Molecular Biology Authors: Jesse DonovanPaul Copeland Source Type: journals
Evolutionary conservation and changes in insect TRP channelsEmail this article to a colleague.
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Conclusions:
The total number of insect TRP family members is 13-14, approximately half that of mammalian TRP family members. As shown for mammalian TRP channels, this may suggest that single TRP channels are responsible for integrating diverse sensory inputs to maintain the insect sensory systems. The above results demonstrate that there are both evolutionary conservation and changes in insect TRP channels. In particular, the evolutionary processes have been accelerated in the TRPA subfamily, indicating divergence in the mechanisms that insects use to detect environmental temperatures.
Source: BMC Evolutionary Biology - Latest articles - September 9, 2009 Category: Molecular Biology Authors: Hironori MatsuuraTakaaki SokabeKeigo KohnoMakoto TominagaTatsuhiko Kadowaki Source Type: journals
Automated organelle-based colocalization in whole-cell imagingEmail this article to a colleague.
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This study clearly demonstrated its value for investigating subcellular structures and their constituent proteins. © 2009 International Society for Advancement of Cytometry
Source: Cytometry Part A - September 9, 2009 Category: Molecular Biology Authors: Ben J. Woodcroft, Luke Hammond, Jennifer L. Stow, Nicholas A. Hamilton Source Type: journals
Identification and Isolation of an Azoreductase from Enterococcus faecium.Email this article to a colleague.
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Azo dyes are commonly used in many commercial industries. Some of the azo dyes can produce carcinogenic compounds after being metabolized by azoreductase. Several human intestinal microbiota possess azoreductase activity which plays an important role in the toxicity and mutagenicity of these azo dye compounds. The acpD gene product (AzoEf1) responsible for the azoreductase activity of Enterococcus faecium, an intestinal bacterium, was heterologously expressed, purified and characterized. The protein sequence shares 67% identity with the azoreductase from Enterococcus faecalis, AzoA. Although AzoEf1 possesses many commo...
Source: Current Issues in Molecular Biology - September 9, 2009 Category: Molecular Biology Authors: Macwana SR, Punj S, Cooper J, Schwenk E, John GH Tags: Curr Issues Mol Biol Source Type: journals
Platelet factor 4 is highly upregulated in dendritic cells after severe trauma.Email this article to a colleague.
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Dendritic cells (DC) represent an important linkage between the innate and adoptive immune system and express pro-inflammatory transcriptomic products early after trauma. Using a genomic approach, platelet factor 4 (PF4) was recently found significantly up-regulated in DCs following multiple trauma. However, knowledge about subsequent PF4 alteration and its potential clinical relevance in the context of multiple trauma is still limited.PF4 expression was analysed both in myeloid (MDC) and plasmocytoid (PDC) DCs isolated from 10 patients after multiple trauma by means of quantitative RT-PCR. Intracellular PF4 as well as...
Source: Molecular Medicine - September 9, 2009 Category: Molecular Biology Authors: Maier M, Geiger EV, Henrich D, Bendt C, Wutzler S, Lehnert M, Marzi I Tags: Mol Med Source Type: journals
Bioactive TGF-beta levels can be preserved in plasma samples collected into heparin but not EDTA.Email this article to a colleague.
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Quantifying TGF-beta is important for many research areas since its effects often are dose-dependently bidirectional. The post-transcriptional control of TGF-beta bioavailability points out the need to determine TGF-beta at the protein level. Studies measuring TGF-beta in peripheral blood have to avoid contamination with platelet-derived TGF-beta. Techniques to obtain platelet-poor plasma have been suggested, however, the impact of different anti-coagulants on artificial TGF-beta contamination has not been studied in detail. Here, we compare TGF-beta levels in blood samples collected into heparin and EDTA tubes, stored...
Source: Cytokine - September 9, 2009 Category: Molecular Biology Authors: Walther M, Jallow IK, Jeffries D, Walther B Tags: Cytokine Source Type: journals
A comparative analysis of the complete mitochondrial genome of the Eurasian otter Lutra lutra (Carnivora; Mustelidae).Email this article to a colleague.
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Otter populations are declining throughout the world and most otter species are considered endangered. Molecular methods are suitable tools for population genetic research on endangered species. In the present study, we analyzed the complete mitochondrial genome (mitogenome) sequence of the Eurasian otter Lutra lutra. The mitochondrial DNA sequence of the Eurasian otter is 16,505 bp in length and consists of 13 protein-coding genes, 22 tRNAs, 2 rRNAs, and a control region (CR). The CR sequence of otters from Europe and Asia showed nearly identical numbers and nucleotide sequences of minisatellites. Phylogenetic analysi...
Source: Molecular Biology Reports - September 9, 2009 Category: Molecular Biology Authors: Ki JS, Hwang DS, Park TJ, Han SH, Lee JS Tags: Mol Biol Rep Source Type: journals
Centrosomes and myeloma; Aneuploidy and proliferationEmail this article to a colleague.
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Multiple myeloma is the second most common hematological malignancy in the United States. The disease is characterized by an accumulation of clonal plasma cells. Clinically, patients present with anemia, lytic bone lesions, hypercalcaemia, or renal impairment. The genome of the malignant plasma cells is extremely unstable and is typically aneuploid and characterized by a complex combination of structure and numerical abnormalities. The basis of the genomic instability underlying myeloma is unclear. In this regard, centrosome amplification is present in about a third of myeloma and may represent a mechanism leading to genom...
Source: Environmental and Molecular Mutagenesis - September 8, 2009 Category: Molecular Biology Authors: Wee J. Chng, Rafael Fonseca Source Type: journals
Approaching clinical proteomics: Current state and future fields of application in cellular proteomicsEmail this article to a colleague.
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In this report, we discuss the recent advances and applications that fulfill the criteria for clinical proteomics with the focus on cellular proteomics (cytoproteomics) as related to preanalytical and analytical standardization and to quality control measures required for effective implementation of these technologies and analytes into routine laboratory testing to generate novel actionable health information. It will then be crucial to design and carry out clinical studies that can eventually identify novel clinical diagnostic strategies based on these techniques and validate their impact on clinical decision making. © 2...
Source: Cytometry Part A - September 8, 2009 Category: Molecular Biology Authors: Rolf Apweiler, Charalampos Aslanidis, Thomas Deufel, Andreas Gerstner, Jens Hansen, Dennis Hochstrasser, Roland Kellner, Markus Kubicek, Friedrich Lottspeich, Edmund Maser, Hans-Werner Mewes, Helmut E. Meyer, Stefan Müllner, Wolfgang Mutter, Michael Neum Source Type: journals
Selective recognition of tetrahedral dianions by a hexaaza cryptand receptorEmail this article to a colleague.
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Pedro Mateus, Rita Delgado, Paula Brandao, Silvia Carvalho, Vitor Felix
(Paper from Org. Biomol. Chem.)
Pedro Mateus, Org. Biomol. Chem., 2009, DOI: 10.1039/b912940e
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Source: RSC - Organic Biomolecular Chemistry - September 8, 2009 Category: Molecular Biology Authors: Pedro MateusRita Delgado Paula Brandao Silvia Carvalho Vitor Felix Source Type: journals
Development of highly sensitive and selective molecules for detection of spermidine and spermineEmail this article to a colleague.
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Daisuke Tanima, Yoko Imamura, Takeo Kawabata, Kazunori Tsubaki
(Paper from Org. Biomol. Chem.)
Daisuke Tanima, Org. Biomol. Chem., 2009, DOI: 10.1039/b909682e
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Source: RSC - Organic Biomolecular Chemistry - September 8, 2009 Category: Molecular Biology Authors: Daisuke TanimaYoko Imamura Takeo Kawabata Kazunori Tsubaki Source Type: journals
Fast optimization of statistical potentials for structurally constrained phylogenetic modelsEmail this article to a colleague.
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Conclusions:
Due to its computational speed, the optimization method we propose offers an attractive alternative for the design and empirical evaluation of alternative forms of potentials, using large data sets and high-dimensional parameterizations.
Source: BMC Evolutionary Biology - Latest articles - September 8, 2009 Category: Molecular Biology Authors: Cecile BonnardClaudia KleinmanNicolas RodrigueNicolas Lartillot Source Type: journals
FGFRL1 is a neglected putative actor of the FGF signalling pathway present in all major metazoan phylaEmail this article to a colleague.
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Conclusions:
Our work sheds light on the existence of a putative FGF signalling pathway actor present in the ancestor of probably all metazoans, the function of which has received little attention until now.
Source: BMC Evolutionary Biology - Latest articles - September 8, 2009 Category: Molecular Biology Authors: Stephanie BertrandIldiko SomorjaiJordi Garcia-FernandezThomas LamonerieHector Escriva Source Type: journals
Identification of a NCR+/NKG2D+/LFA-1low/CD94- immature human NK cell subsetEmail this article to a colleague.
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CD56bright natural killer (NK) cells, generated in vitro from CD34+ hematopoietic progenitor cells, were characterized after a 30-day culture with flt3 ligand plus IL-15. Virtually, all CD56bright cells expressed CD117, CD25, natural cytotoxicity receptors (NCRs), NKG2D, CD161, and CD244, while only a subset expressed CD18-CD11a (LFA-1), and CD94 molecule, defining an immature CD56bright/NCRs+/NKG2D+/LFA-1-/CD94- subset. Another small subset of cells expressing CD94 but not LFA-1 integrin was also identified, suggesting that during NK differentiation LFA-1 might be upregulated later than CD94. To verify this hypothesis in ...
Source: Cytometry Part A - September 8, 2009 Category: Molecular Biology Authors: Loris Zamai, Laura Galeotti, Genny Del Zotto, Barbara Canonico, Prisco Mirandola, Stefano Papa Source Type: journals
Metabolism as means for hypoxia adaptation: metabolic profiling and flux balance analysisEmail this article to a colleague.
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Conclusions:
We suggest a network-level hypothesis of metabolic regulation in hypoxia-adapted flies, in which lower baseline rates of biosynthesis in adapted flies draws less anaplerotic flux, resulting in lower rates of glycolysis, less acidosis, and more efficient use of substrate during acute hypoxic stress. In addition we suggest new specific hypothesis, which were found to be consistent with existing data.
Source: BMC Systems Biology - Latest articles - September 8, 2009 Category: Molecular Biology Authors: Jacob FealaLaurence CoquinDan ZhouGabriel HaddadGiovanni PaternostroAndrew McCulloch Source Type: journals
Gas phase optical rotation calculated from coupled cluster theory with zero-point vibrational corrections from density funcional theory.Email this article to a colleague.
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Molecular vibrations can have a significant influence on gas phase specific optical rotations. Mainly due to the large number of nuclear degrees of freedom in most chiral molecules, theoretical predictions of vibrational corrections quickly become prohibitively expensive. Here, we investigate an approach in which the purely electronic contribution is calculated at the coupled cluster singles and doubles level, while the zero-point vibrational correction is computed using the less demanding density functional theory (B3LYP functional). By comparing to experimental gas phase results for seven molecules and two wavelength...
Source: Chirality - September 8, 2009 Category: Molecular Biology Authors: Pedersen TB, Kongsted J, Crawford TD Tags: Chirality Source Type: journals
Determination of the absolute configuration of the natural product klaivanolide via density functional calculations of vibrational circular dichroism (VCD).Email this article to a colleague.
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The absolute configuration (AC) of the antiprotozoal lactone, Klaivanolide, 1, from Uvaria klaineana, has been determined using Vibrational Circular Dichroism (VCD) spectroscopy. The experimental VCD spectrum of the (+) enantiomer of 1 was measured. To analyze the AC of (+)-1, the conformationally-averaged VCD spectrum of 7-S-1 was calculated using density functional theory (DFT) and the GAUSSIAN 03 program. The B3PW91/TZ2P conformationally-averaged VCD spectrum of 7-S-1 proves that the AC of 1 is 7-S-(+). Chirality 2009. (c) 2009 Wiley-Liss, Inc.
PMID: 19743486 [PubMed - as supplied by publisher]
Source: Chirality - September 8, 2009 Category: Molecular Biology Authors: Devlin FJ, Stephens PJ, Figadère B Tags: Chirality Source Type: journals
High-performance liquid chromatography separation of enantiomers of mandelic acid and its analogs on a chiral stationary phase.Email this article to a colleague.
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The enantiomers of mandelic acid and its analogs have been chromatographically separated on a chiral stationary phase (CSP) derived from 4-(3,5-dinitrobenzamido) tetrahydrophenanthrene. The rationale of separations of these compounds is discussed with respect to the method development for determining enantiomeric purity and possibility of obtaining enantiomerically pure materials by high-pressure liquid chromatography. The relationship of analyte structure to the extent of enantiomeric separation has been examined and separation factors (alpha) are presented for various groups of structurally related compounds. Chiral ...
Source: Chirality - September 8, 2009 Category: Molecular Biology Authors: Aneja R, Luthra PM, Ahuja S Tags: Chirality Source Type: journals
Production of (R)-1-phenylethanols through bioreduction of acetophenones by a new fungus isolate Trichothecium roseum.Email this article to a colleague.
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A total of 120 fungal strains were isolated from soil samples and evaluated in the bioreduction of substituted acetophenones to the corresponding (R)-alcohols. Among these strains, isolate Trichothecium roseum EBK-18 was highly effective in the production of (R)-alcohols with excellent enantioselectivity (ee > 99%). Gram scale preparation of (R)-1-phenylethanol is reported. Chirality 2009. (c) 2009 Wiley-Liss, Inc.
PMID: 19743484 [PubMed - as supplied by publisher]
Source: Chirality - September 8, 2009 Category: Molecular Biology Authors: Zilbeyaz K, Taskin M, Kurbanoglu EB, Kurbanoglu NI, Kilic H Tags: Chirality Source Type: journals
Bile acids in asymmetric synthesis and chiral discrimination.Email this article to a colleague.
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An overview on the use of bile acid-based compounds able to catalyze transformations, control the stereochemical course of a given reaction, recognize and bind other molecules, is presented. The recent developments in inclusion discrimination of chiral and achiral guests and enantioselective recognition achieved by bile acid are described with suitable examples. Chirality 2009. (c) 2009 Wiley-Liss, Inc.
PMID: 19743483 [PubMed - as supplied by publisher]
Source: Chirality - September 8, 2009 Category: Molecular Biology Authors: Bortolini O, Fantin G, Fogagnolo M Tags: Chirality Source Type: journals
Imaging chiroptical artifacts.Email this article to a colleague.
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It is well-known that circular dichroism (CD) measurements of anisotropic media may contain artifacts that result from mixed linear anisotropies. Such artifacts are generally considered a nuisance. However, systematic artifacts, carefully measured, may contain valuable information. Herein, polycrystalline spherulites of D-sorbitol grown from the melt were analyzed with a Mueller matrix microscope, among other differential polarization images devices. As spherulites grew into one another they developed strong apparent optical rotation and CD signals at the boundaries between spherulites. These signals are shown not to h...
Source: Chirality - September 8, 2009 Category: Molecular Biology Authors: Freudenthal JH, Hollis E, Kahr B Tags: Chirality Source Type: journals
From PKA to HCN: The cAMP-capture compound mass spectrometry as a novel tool for targeting cAMP binding proteins.Email this article to a colleague.
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We present here the design, synthesis, and application of a new Capture Compound to target and identify cAMP binding proteins in complex protein mixtures. Starting with modest amounts of total protein mixture (65 microg-500 microg), we demonstrate that the cAMP Capture Compounds (cAMP-CCs) can be used to isolate bona fide cAMP binding proteins from lysates of Escherichia coli, mammalian HepG2 cells, and subcellular fractions of mammalian brain, respectively. The identified proteins captured by the cAMP-CC range from soluble cAMP binding proteins, such as the catabolite gene activator protein from E. coli and regulatory sub...
Source: Molecular and Cellular Proteomics : MCP - September 8, 2009 Category: Molecular Biology Authors: Luo Y, Blex C, Baessler O, Glinski M, Dreger M, Sefkow M, Köster H Tags: Mol Cell Proteomics Source Type: journals
In vitro and in vivo protein-bound tyrosine nitration characterized by diagonal chromatography.Email this article to a colleague.
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A new proteomics technique for analyzing 3-nitrotyrosine-containing peptides is presented here. This technique is based on the COFRADIC (combined fractional diagonal chromatography) peptide isolation procedures by which specific classes of peptides are isolated following a series of identical reverse-phase HPLC separation steps. Dithionite is here used to reduce 3-nitrotyrosine to 3-aminotyrosine peptides which thereby become more hydrophilic. Our COFRADIC technique was first applied to characterize tyrosine nitration in tetranitromethane-modified BSA and further led to a high quality list of 335 tyrosine nitration sit...
Source: Molecular and Cellular Proteomics : MCP - September 8, 2009 Category: Molecular Biology Authors: Ghesquière B, Colaert N, Helsens K, Dejager L, Vanhaute C, Verleysen K, Kas K, Timmerman E, Goethals M, Libert C, Vandekerckhove J, Gevaert K Tags: Mol Cell Proteomics Source Type: journals
Myosin Vc Is a Molecular Motor That Functions in Secretory Granule Trafficking.Email this article to a colleague.
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Monitoring Editor: Thomas F.J. Martin Class V myosins are actin-based motor proteins that have critical functions in organelle trafficking. Of the three class V myosins expressed in mammals, relatively little is known about Myo5c except that it is abundant in exocrine tissues. Here we use MCF-7 cells to identify the organelles that Myo5c associates with, image the dynamics of Myo5c in living cells, and test the functions of Myo5c. Endogenous Myo5c localizes to two distinct compartments, small puncta and slender tubules. Myo5c often exhibits a highly polarized distribution toward the leading edge in migrating cells and ...
Source: Mol Biol Cell - September 8, 2009 Category: Molecular Biology Authors: Jacobs DT, Weigert R, Grode KD, Donaldson JG, Cheney RE Tags: Mol Biol Cell Source Type: journals
Mitochondrial Ubiquitin Ligase MITOL Ubiquitinates Mutant SOD1 and Attenuates Mutant SOD1-induced ROS Generation.Email this article to a colleague.
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In this study, we examined whether MITOL eliminates misfolded proteins localized to mitochondria. Mutant superoxide dismutase1 (mSOD1), one of misfolded proteins, has been shown to localize in mitochondria and induce mitochondrial dysfunction, possibly involving in the onset and progression of amyotrophic lateral sclerosis. We found that in the mitochondria, MITOL interacted with and ubiquitinated mSOD1 but not wild type SOD1. In vitro ubiquitination assay revealed that MITOL directly ubiquitinates mSOD1. Cycloheximide-chase assay in the Neuro2a cells indicated that MITOL overexpression promoted mSOD1 degradation and suppr...
Source: Mol Biol Cell - September 8, 2009 Category: Molecular Biology Authors: Yonashiro R, Sugiura A, Miyachi M, Fukuda T, Matsushita N, Inatome R, Ogata Y, Suzuki T, Dohmae N, Yanagi S Tags: Mol Biol Cell Source Type: journals
Unbalancing the PI(4,5)P2-Cofilin Interaction Impairs Cell Steering.Email this article to a colleague.
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Monitoring Editor: Carole Parent Cofilin is a key player in actin dynamics during cell migration. Its activity is regulated by (de)phosphorylation, pH and binding to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2). We here use a human cofilin-1 (D122K) mutant with increased binding affinity for PI(4,5)P2 and slower release from the plasma membrane to study the role of the PI(4,5)P2-cofilin interaction in migrating cells. In fibroblasts in a background of endogenous cofilin, D122K cofilin expression negatively affects cell turning frequency. In carcinoma cells with down-regulated endogenous cofilin, D122K cofilin neit...
Source: Mol Biol Cell - September 8, 2009 Category: Molecular Biology Authors: Leyman S, Sidani M, Ritsma L, Waterschoot D, Eddy R, Dewitte D, Debeir O, Decaestecker C, Vandekerckhove J, van Rheenen J, Ampe C, Condeelis J, Van Troys M Tags: Mol Biol Cell Source Type: journals
Cdc42 Regulates Fc{gamma} Receptor-mediated Phagocytosis through the Activation and Phosphorylation of WASP and N-WASP.Email this article to a colleague.
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Monitoring Editor: Carole Parent Cdc42 is a key regulator of the actin cytoskeleton and activator of Wiskott-Aldrich syndrome protein (WASP). Although several studies have separately demonstrated the requirement for both Cdc42 and WASP in Fcgamma receptor (FcgammaR)-mediated phagocytosis, their precise roles in the signal cascade leading to engulfment are still unclear. Reduction of endogenous Cdc42 expression using RNA-mediated interference (shRNA) severely impaired the phagocytic capacity of RAW/LR5 macrophages, due to defects in phagocytic cup formation, actin assembly and pseudopod extension. Addition of wiskostati...
Source: Mol Biol Cell - September 8, 2009 Category: Molecular Biology Authors: Park H, Cox D Tags: Mol Biol Cell Source Type: journals
HOPS Interacts with Apl5 at the Vacuole Membrane and Is Required for Consumption of AP-3 Transport Vesicles.Email this article to a colleague.
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Monitoring Editor: Sandra Lemmon Adaptor Protein complexes (APs) are evolutionarily conserved heterotetramers that couple cargo selection to the formation of highly curved membranes during vesicle budding. In S. cerevisiae, AP-3 mediates vesicle traffic from the late Golgi to the vacuolar lysosome. The HOPS subunit Vps41 is one of the few proteins reported to have a specific role in AP-3 traffic, yet its function remains undefined. We now show that although the AP-3 delta subunit, Apl5, binds Vps41 directly, this interaction occurs preferentially within the context of the HOPS docking complex. Fluorescence microscopy i...
Source: Mol Biol Cell - September 8, 2009 Category: Molecular Biology Authors: Angers CG, Merz AJ Tags: Mol Biol Cell Source Type: journals
Physical and Functional Interaction of Transmembrane Thioredoxin-related Protein (TMX) with MHC Class I Heavy Chain: Redox-based Protein Quality Control and Its Potential Relevance to Immune Responses.Email this article to a colleague.
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Monitoring Editor: Ramanujan S. Hegde In the endoplasmic reticulum (ER), a variety of oxidoreductases classified in the thioredoxin superfamily have been found to catalyze the formation and rearrangement of disulfide bonds. However, the precise function and specificity of the individual thioredoxin family proteins remain to be elucidated. Here, we characterize a transmembrane thioredoxin-related protein (TMX), a membrane bound oxidoreductase in the ER. TMX exists in a predominantly reduced form and associates with the molecular chaperon calnexin, which can mediate substrate binding. To determine the target molecules fo...
Source: Mol Biol Cell - September 8, 2009 Category: Molecular Biology Authors: Matsuo Y, Masutani H, Son A, Kizaka-Kondoh S, Yodoi J Tags: Mol Biol Cell Source Type: journals
Molecular modeling and docking studies of human 5-hydroxytryptamine 2A (5-HT2A) receptor for the identification of hotspots for ligand bindingEmail this article to a colleague.
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Karuppiah Kanagarajadurai, Manoharan Malini, Aditi Bhattacharya, Mitradas M. Panicker, Ramanathan Sowdhamini
(Paper from Mol. BioSyst.)
Karuppiah Kanagarajadurai, Mol. BioSyst., 2009, DOI: 10.1039/b906391a
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Source: RSC - Mol. BioSyst. latest articles - September 7, 2009 Category: Molecular Biology Authors: Karuppiah KanagarajaduraiManoharan Malini Aditi Bhattacharya Mitradas M. Panicker Ramanathan Sowdhamini Source Type: journals
Explorations in topology-delving underneath the surface of genetic interaction mapsEmail this article to a colleague.
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Michal Breker, Maya Schuldiner
(Review from Mol. BioSyst.)
Michal Breker, Mol. BioSyst., 2009, DOI: 10.1039/b907076c
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Source: RSC - Mol. BioSyst. latest articles - September 7, 2009 Category: Molecular Biology Authors: Michal BrekerMaya Schuldiner Source Type: journals
Ionic liquid: an efficient and recyclable medium for synthesis of unsymmetrical diorganyl selenides promoted by InIEmail this article to a colleague.
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Senthil Narayanaperumal, Eduardo E. Alberto, Fabiano Molinos de Andrade, Eder J. Lenardao, Paulo S. Taube, Antonio L. Braga
(Paper from Org. Biomol. Chem.)
Senthil Narayanaperumal, Org. Biomol. Chem., 2009, DOI: 10.1039/b910699e
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Source: RSC - Organic Biomolecular Chemistry - September 7, 2009 Category: Molecular Biology Authors: Senthil NarayanaperumalEduardo E. Alberto Fabiano Molinos de Andrade Eder J. Lenardao Paulo S. Taube Antonio L. Braga Source Type: journals
Chiral amine/chiral acid as an excellent organocatalytic system for the enantioselective tandem oxa-Michael-aldol reactionEmail this article to a colleague.
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Shu-Ping Luo, Zhao-Bo Li, Li-Ping Wang, Yi Guo, Ai-Bao Xia, Dan-Qian Xu
(Paper from Org. Biomol. Chem.)
Shu-Ping Luo, Org. Biomol. Chem., 2009, DOI: 10.1039/b910835a
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Source: RSC - Organic Biomolecular Chemistry - September 7, 2009 Category: Molecular Biology Authors: Shu-Ping LuoZhao-Bo Li Li-Ping Wang Yi Guo Ai-Bao Xia Dan-Qian Xu Source Type: journals
N-Hexyl-4-aminobutyl glycosides for investigating structures and biological functions of carbohydratesEmail this article to a colleague.
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Katsuhiko Suzuki, Akifumi Tobe, Shin Adachi, Shusaku Daikoku, Yasuko Hasegawa, Yuki Shioiri, Mariko Kobayashi, Osamu Kanie
(Paper from Org. Biomol. Chem.)
Katsuhiko Suzuki, Org. Biomol. Chem., 2009, DOI: 10.1039/b909556j
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Source: RSC - Organic Biomolecular Chemistry - September 7, 2009 Category: Molecular Biology Authors: Katsuhiko SuzukiAkifumi Tobe Shin Adachi Shusaku Daikoku Yasuko Hasegawa Yuki Shioiri Mariko Kobayashi Osamu Kanie Source Type: journals
