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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 28.

PROCOV: maximum likelihood estimation of protein phylogeny under covarion models and site-specific covarion pattern analysisemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Covarion models implemented in PROCOV may be especially useful for phylogenetic estimation when ancient divergences between sequences have occurred and rates of evolution at sites are likely to have changed over the tree. It can also be used to study lineage-specific functional shifts in protein families that result in changes in the patterns of site variability among subtrees.
Source: BMC Evolutionary Biology - Latest articles - September 7, 2009 Category: Molecular Biology Authors: Huai-Chun WangEdward SuskoAndrew Roger Source Type: journals

The identification and functional implications of human-specific "fixed" amino acid substitutions in the glutamate receptor familyemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: We conclude that the GluR genes did not undergo drastic changes such as accelerated evolution in the human lineage after the divergence of chimpanzees. However, there remains a possibility that two human-specific "fixed" amino acid substitutions, D71G in GRIN3A and R727H in GRIN3B, are related to human-specific brain function.
Source: BMC Evolutionary Biology - Latest articles - September 7, 2009 Category: Molecular Biology Authors: Hiroki GotoKazunori WatanabeNaozumi AraragiRui KageyamaKunika TanakaYoko KurokiAtsushi ToyodaMasahira HattoriYoshiyuki SakakiAsao FujiyamaYasuyuki FukumakiHiroki Shibata Source Type: journals

The smallest chemical reaction system with bistabilityemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The presented minimal bistable system finally clarifies the often discussed question for the necessary conditions for bistability. The three necessary conditions are: positive feedback, a mechanism to filter out small stimuli and a mechanism to prevent explosions. This is important for modelling bistability with simple systems and for synthetically designing new bistable systems. Our simple model system is also well suited for corresponding teaching purposes.
Source: BMC Systems Biology - Latest articles - September 7, 2009 Category: Molecular Biology Authors: Thomas Wilhelm Source Type: journals

Rac1 activation inhibits E-cadherin-mediated adherens junctions via binding to IQGAP1 in pancreatic carcinoma cellsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: These results indicate that active Rac1 destabilises E-cadherin-mediated cell-cell adhesion in pancreatic carcinoma cells by interacting with IQGAP1 which is associated with a disassembly of E-cadherin-mediated adherens junctions. Inhibition of Rac1 activity induced increased E-cadherin-mediated cellular adhesion.
Source: Cell Communication and Signaling - September 7, 2009 Category: Molecular Biology Authors: Beatrix HageKatrin MeinelIris BaumKlaudia GiehlAndre Menke Source Type: journals

Function, regulation and pathological roles of the Gab/DOS docking proteinsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Since their discovery a little more than a decade ago, the docking proteins of the Gab/DOS family have emerged as important signalling elements in metazoans. Gab/DOS proteins integrate and amplify signals from a wide variety of sources including growth factor, cytokine and antigen receptors as well as cell adhesion molecules. They also contribute to signal diversification by channelling the information from activated receptors into signalling pathways with distinct biological functions. Recent approaches in protein biochemistry and systems biology have revealed that Gab proteins are subject to complex regulation by feed-fo...
Source: Cell Communication and Signaling - September 7, 2009 Category: Molecular Biology Authors: Franziska WoehrleRoger DalyTilman Brummer Source Type: journals

Recombinant adeno-associated virus serotype 6 (rAAV2/6)-mediated gene transfer to nociceptive neurons through different routes of deliveryemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: We have found that rAAV2/6 is an efficient vector to deliver transgenes to nociceptive neurons in mice. Furthermore, the characterization of the transduction profile may facilitate gene transfer studies to dissect mechanisms behind neuropathic pain.
Source: Molecular Pain - September 7, 2009 Category: Molecular Biology Authors: Chris TowneMarie PertinAhmed BeggahPatrick AebischerIsabelle Decosterd Source Type: journals

Homage to Theodor Boveri (1862-1915): Boveri's theory of cancer as a disease of the chromosomes, and the landscape of genomic imbalances in human carcinomasemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
No abstract.
Source: Environmental and Molecular Mutagenesis - September 7, 2009 Category: Molecular Biology Authors: Thomas Ried Source Type: journals

Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanismemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
This study demonstrates that the APC-tandem dyes are the target of cell-dependent degradation, which may be antagonized. These findings will allow cytometer users to optimize their multicolor panels. © 2009 International Society for Advancement of Cytometry
Source: Cytometry Part A - September 7, 2009 Category: Molecular Biology Authors: Christine Le Roy, Nadine Varin-Blank, Florence Ajchenbaum-Cymbalista, Rémi Letestu Source Type: journals

An optimized flow cytometry protocol for analysis of angiogenic monocytes and endothelial progenitor cells in peripheral bloodemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Circulating adult CD34+VEGFR2+ endothelial progenitor cells (EPCs) have been shown to differentiate into endothelial cells, thus contributing to vascular homeostasis. Furthermore, a subset of circulating CD14+ monocytes coexpresses CD16 together with the angiopoietin receptor Tie2 and has been functionally implicated in tumor angiogenesis. However, clinically applicable protocols for flow cytometric quantification of EPCs and Tie2+ monocytes in peripheral blood and a consensus on reference values remain elusive. The number of Tie2+CD14+CD16mid angiogenic monocytes and CD34+VEGFR2+CD45low/- EPCs was assessed in the peripher...
Source: Cytometry Part A - September 7, 2009 Category: Molecular Biology Authors: Mihail Hristov, Susanne Schmitz, Christoph Schuhmann, Thorsten Leyendecker, Philipp von Hundelshausen, Florian Krötz, Hae-Young Sohn, Frans A. Nauwelaers, Christian Weber Source Type: journals

Structure of membrane-associated neuronal SNARE complex: Implication in neurotransmitter release.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Abstract To enable fusion between biological membranes, t-SNAREs and v-SNARE present in opposing bilayers, interact and assemble in a circular configuration forming ring-complexes, which establish continuity between the opposing membranes, in presence of calcium ions. The size of a t-/v-SNARE ring complex is dictated by the curvature of the opposing membrane. Hence smaller vesicles form small SNARE-ring complexes, as opposed to large vesicles. Neuronal communication depends on the fusion of 40-50 nm in diameter membrane-bound synaptic vesicles containing neurotransmitters at the nerve terminal. At the presynaptic membr...
Source: J Cell Mol Med - September 7, 2009 Category: Molecular Biology Authors: Cho WJ, Shin L, Ren G, Jena BP Tags: J Cell Mol Med Source Type: journals

Adrenomedullin Promotes Lung Angiogenesis, Alveolar Development and Repair.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Bronchopulmonary dysplasia (BPD) and emphysema are significant global health problems at the extreme stages of life. Both are characterized by alveolar simplification and abnormal distal airspace enlargement due to arrested development or loss of alveoli, respectively. Both lack effective treatments. Mechanisms that inhibit distal lung growth are poorly understood. Adrenomedullin (AM), a recently discovered potent vasodilator, promotes angiogenesis and has protective effects on the cardiovascular and respiratory system. Its role in the developing lung is unknown. We hypothesized that AM promotes lung angiogenesis and a...
Source: American Journal of Respiratory Cell and Molecular Biology - September 7, 2009 Category: Molecular Biology Authors: Vadivel A, Abozaid S, van Haaften T, Sawicka M, Eaton F, Chen M, Thebaud B Tags: Am J Respir Cell Mol Biol Source Type: journals

Mechanisms of Neutrophil Accumulation in the Lungs Against Bacteria.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Bacterial lung diseases are a major cause of morbidity and mortality both in immunocompromised and immunocompetent individuals. Neutrophil accumulation, a pathological hallmark of bacterial diseases, is critical to host defense, but may also cause ALI/ARDS. Toll-like receptors, NOD-like receptors, transcription factors, cytokines, and chemokines play essential roles in neutrophil sequestration in the lungs. This review highlights our current understanding of the role of these molecules in the lungs during bacterial infection and their therapeutic potential. We also discuss emerging data on cholesterol and ethanol as en...
Source: American Journal of Respiratory Cell and Molecular Biology - September 7, 2009 Category: Molecular Biology Authors: Balamayooran G, Batra S, Fessler MB, Happel KI, Jeyaseelan S Tags: Am J Respir Cell Mol Biol Source Type: journals

Bacterial Lipopolysaccharide Enhances PDGF Signaling and Pulmonary Fibrosis in Rats Exposed to Carbon Nanotubes.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Engineered multi-walled carbon nanotubes (MWCNT) represent a possible health risk for pulmonary fibrosis due to their fiber-like shape and potential for persistence in the lung. We postulated that bacterial lipopolysaccharide (LPS), a ubiquitous agent in the environment that causes lung inflammation, would enhance fibrosis caused by MWCNT. Rats were exposed to LPS and then intratracheally instilled with MWCNT or carbon black (CB) nanoparticles 24 hrs later. Pulmonary fibrosis was observed 21 days post-MWCNT exposure, but not with CB. LPS alone caused no fibrosis but enhanced MWCNT-induced fibrosis. LPS plus CB did not ...
Source: American Journal of Respiratory Cell and Molecular Biology - September 7, 2009 Category: Molecular Biology Authors: Cesta MF, Ryman-Rasmussen JP, Wallace DG, Masinde T, Hurlburt G, Taylor AJ, Bonner JC Tags: Am J Respir Cell Mol Biol Source Type: journals

Cross-species analysis of the glycolytic pathway by comparison of molecular interaction fieldsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Matthias Stein, Razif R. Gabdoulline, Rebecca C. Wade (Paper from Mol. BioSyst.) Matthias Stein, Mol. BioSyst., 2009, DOI: 10.1039/b912398a To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 6, 2009 Category: Molecular Biology Authors: Matthias SteinRazif R. Gabdoulline Rebecca C. Wade Source Type: journals

Synthetic dinucleotide mRNA cap analogs with tetraphosphate 5[prime or minute],5[prime or minute] bridge containing methylenebis(phosphonate) modificationemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Anna Maria Rydzik, Maciej Lukaszewicz, Joanna Zuberek, Joanna Kowalska, Zbigniew Marek Darzynkiewicz, Edward Darzynkiewicz, Jacek Jemielity (Paper from Org. Biomol. Chem.) Anna Maria Rydzik, Org. Biomol. Chem., 2009, DOI: 10.1039/b911347a To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - September 6, 2009 Category: Molecular Biology Authors: Anna Maria RydzikMaciej Lukaszewicz Joanna Zuberek Joanna Kowalska Zbigniew Marek Darzynkiewicz Edward Darzynkiewicz Jacek Jemielity Source Type: journals

FoxO gene family evolution in vertebratesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: We present a phylogeny describing the evolutionary history of the FoxO gene family and show that the genes have evolved through duplications followed by purifying selection except for four sites in FoxO6 fixed by positive selection lie mostly within the non-conserved optimal PKB motif in the C-terminal part. Relaxed selection may play important roles in the process of functional differentiation evolved through gene duplications as well.
Source: BMC Evolutionary Biology - Latest articles - September 6, 2009 Category: Molecular Biology Authors: Minghui WangXiangzhe ZhangHongbo ZhaoQishan WangYuchun Pan Source Type: journals

Hybrid stochastic simplifications for multiscale gene networksemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Hybrid simplifications can be used for onion-like (multilayered) approaches to multi-scale biochemical systems, in which various descriptions are used at various scales. Sets of discrete and continuous variables are treated with different methods and are coupled together in a physically justified approach.
Source: BMC Systems Biology - Latest articles - September 6, 2009 Category: Molecular Biology Authors: Alina CruduArnaud DebusscheOvidiu Radulescu Source Type: journals

A systems biology approach to investigate the response of Synechocystis sp. PCC6803 to a high salt environmentemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Synechocystis sp. PCC6803 has the ability to regulate essential metabolic processes to enable survival in high salt environments. This adaptation strategy is assisted by further regulation of proteins involved in non-metabolic cellular processes, supported by transcriptional and post-transcriptional control. This study demonstrates the effectiveness of using a systems biology approach in answering environmental, and in particular, salt adaptation questions in Synechocystis sp. PCC6803.
Source: Saline Systems - September 6, 2009 Category: Molecular Biology Authors: Jagroop PandhalJosselin NoirelPhillip WrightCatherine Biggs Source Type: journals

Mechanisms of bone repair and regeneration.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Bone problems can have a highly deleterious impact on life and society, therefore understanding the mechanisms of bone repair is important. In vivo studies show that bone repair processes in adults resemble normal development of the skeleton during embryogenesis, which can thus be used as a model. In addition, recent studies of skeletal stem cell biology have underlined several crucial molecular and cellular processes in bone formation. Hedgehog, parathyroid hormone-related protein, Wnt, bone morphogenetic proteins and mitogen-activated protein kinases are the main molecular players, and osteoclasts and mesenchymal ste...
Source: Trends in Molecular Medicine - September 6, 2009 Category: Molecular Biology Authors: Deschaseaux F, Sensébé L, Heymann D Tags: Trends Mol Med Source Type: journals

A comparison of different purification methods of aggrecan fragments from human articular cartilage and synovial fluid.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
In the study of aggrecan fragmentation several methods to extract and purify aggrecan from cartilage and synovial fluid (SF) are used. This work compares and evaluates the effectiveness for purification of aggrecan of the most commonly used methods by the ratio of sulfated glycosaminoglycan (sGAG) to protein and by fragment analysis by Western blot. A novel method for purification of aggrecan fragments from SF by boiling (Boiled SF) is also presented. Of the sGAG extracted from cartilage by guanidinium, 66% was recovered by associative-dissociative cesium chloride density gradient centrifugation (A1D1-D3) with a 9 time...
Source: Matrix Biology - September 6, 2009 Category: Molecular Biology Authors: Struglics A, Larsson S Tags: Matrix Biol Source Type: journals

Regulation of JNK and p38 MAPK in the immune system: Signal integration, propagation and termination.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Stress-activated MAP kinases (MAPKs), comprised of JNK and p38, play prominent roles in the innate and adaptive immune systems. Activation of MAPKs is mediated by a three-tiered kinase module comprised of MAPK kinase kinases (MAP3Ks), MAPK kinases (MAP2Ks) and MAPKs through sequential protein phosphorylation. Activated MAPKs, in turn, phosphorylate transcription factors and other targets to regulate gene transcription and immune responses. Recent studies have provided new insight into the upstream and downstream components of the MAPK pathway that facilitate the activation and propagation of MAPK signaling in immune re...
Source: Cytokine - September 6, 2009 Category: Molecular Biology Authors: Huang G, Shi LZ, Chi H Tags: Cytokine Source Type: journals

Estrogen-related receptor alpha (ERRalpha) inverse agonist XCT-790 induces cell death in chemotherapeutic resistant cancer cells.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
In this study, we found that an ERRalpha inverse agonist XCT-790 induced cell death in HepG2 hepatocarcinoma and its multi-drug resistance (MDR) sub-line R-HepG2. Using a dye Mitotracker Green which stains mitochondrion independent of mitochondrial membrane potential (DeltaPsi(m)), we found that XCT-790 dose-dependently decreased mitochondrial mass. Intriguingly, XCT-790 increased DeltaPsi(m) upon short term treatment but decreased DeltaPsi(m) upon longer term treatment. The changes of DeltaPsi(m) in turn promoted the production of reactive oxygen species (ROS) and led to ROS-mediated caspases 3/7, 8, 9 activation and cell...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Wu F, Wang J, Wang Y, Kwok TT, Kong SK, Wong C Tags: Chem Biol Interact Source Type: journals

Anti-cataractogenic effect of curcumin and aminoguanidine against selenium-induced oxidative stress in the eye lens of Wistar rat pups: An in vitro study using isolated lens.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
The aim of this study was to investigate whether curcumin and aminoguanidine (AG) prevent selenium-induced cataractogenesis in vitro. On postpartum day 8, transparent isolated lens were incubated in 24 well plates containing Dulbecco's Modified Eagle Medium (DMEM). Isolated lens of group I were incubated with DMEM medium alone. Group II: lenses incubated in DMEM containing 100microM sodium selenite; group III: lenses incubated in DMEM containing 100microM sodium selenite and 100microM curcumin; group IV: lenses incubated in DMEM containing 100microM sodium selenite and 200microM curcumin; group V: lenses incubated in D...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Manikandan R, Thiagarajan R, Beulaja S, Chindhu S, Mariammal K, Sudhandiran G, Arumugam M Tags: Chem Biol Interact Source Type: journals

Combined application of camptothecin and the guanylate cyclase activator YC-1: Impact on cell death and apoptosis-related proteins in ovarian carcinoma cell lines.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Camptothecin analogs and guanylate cyclase activator YC-1 [3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole] have been shown to induce apoptosis in cancer cells. However, the combined effect of camptothecin analogs and YC-1 on the viability of epithelial ovarian cancer cells remains uncertain. We assessed the combined effect of YC-1 on the camptothecin toxicity in the human epithelial ovarian carcinoma cell lines OVCAR-3 and SK-OV-3. Camptothecin and YC-1 induced apoptosis in OVCAR-3 and SK-OV-3 cells in a dose- and time-dependent manner. Both compounds induced nuclear damage, decreased Bid and Bcl-2 protein levels, enh...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Lee SJ, Kim YJ, Lee CS, Bae J Tags: Chem Biol Interact Source Type: journals

Metabolic activation of the phenothiazine antipsychotics chlorpromazine and thioridazine to electrophilic iminoquinone species in human liver microsomes and recombinant P450s.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
The phenothiazine-derived antipsychotics, namely chlorpromazine and thioridazine, have been associated with very rare but severe incidences of hepatotoxicity in patients. While the mechanism of idiosyncratic hepatotoxicity remains unknown, it is possible that metabolic activation and subsequent covalently binding of reactive metabolites to cellular proteins play a causative role. Studies were initiated to determine whether chlorpromazine and thioridazine undergo cytochrome P450 (P450)-mediated bioactivation in human liver microsomes to electrophilic intermediates. LC/MS/MS analysis of incubations containing chlorpromaz...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Wen B, Zhou M Tags: Chem Biol Interact Source Type: journals

Metabolism of carbosulfan. I. Species differences in the in vitro biotransformation by mammalian hepatic microsomes including human.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
The in vitro metabolism of carbosulfan, a widely used carbamate insecticide, by hepatic microsomes from human, rat, mouse, dog, rabbit, minipig, and monkey was studied. Altogether eight (8) phase I metabolites were detected by LC-MS; phase II metabolites were not found in human homogenates fortified with appropriate cofactors. The primary metabolic pathways were the initial oxidation of sulfur to carbosulfan sulfinamide ('sulfur oxidation pathway') and the cleavage of the nitrogen sulfur bond (N-S) to give carbofuran and dibutylamine ('carbofuran pathway'). Carbofuran was further hydroxylated to 3-hydroxycarbofuran and...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Abass K, Reponen P, Mattila S, Pelkonen O Tags: Chem Biol Interact Source Type: journals

Resveratrol ameliorates DNA damage, prooxidant and antioxidant imbalance in 1,2-dimethylhydrazine induced rat colon carcinogenesis.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Colorectal cancer is one of the most common internal malignancies in Western society. Currently oxidative stress has been increasingly postulated as a major contributor to carcinogenesis. The assessment of damage in various biological matrices, such as tissues and cells, is vital to understand the development of carcinogenesis and subsequently devising intervention strategies. Thus, the major objective of the present study was to examine the effect of resveratrol (Res) on DNA damage in a short-term study of 16 days and circulatory lipid peroxidation, enzymic/non-enzymic antioxidants status in a long-term study of 30 we...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Sengottuvelan M, Deeptha K, Nalini N Tags: Chem Biol Interact Source Type: journals

Potent growth suppressive activity of curcumin in human breast cancer cells: Modulation of Wnt/beta-catenin signaling.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
In conclusion, our data demonstrated that the efficacy of curcumin in inhibition of cell proliferation and induction of apoptosis might occur through modulation of beta-catenin pathway in human breast cancer cells. PMID: 19573523 [PubMed - indexed for MEDLINE]
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Prasad CP, Rath G, Mathur S, Bhatnagar D, Ralhan R Tags: Chem Biol Interact Source Type: journals

Fatty acids increase paracellular absorption of aluminium across Caco-2 cell monolayers.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Passive paracellular absorption, regulated by tight junctions (TJs), is the main route for absorption of poorly absorbed hydrophilic substances. Surface active substances, such as fatty acids, may enhance absorption of these substances by affecting the integrity of TJ and increasing the permeability. It has been suggested that aluminium (Al) absorption occurs mainly by the paracellular route. Herein, we investigated if physiologically relevant exposures of fully differentiated Caco-2 cell monolayers to oleic acid and docosahexaenoic acid (DHA), which are fatty acids common in food, increase absorption of Al and the par...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Aspenström-Fagerlund B, Sundström B, Tallkvist J, Ilbäck NG, Glynn AW Tags: Chem Biol Interact Source Type: journals

Bioactivation of coumarin in rat olfactory mucosal microsomes: Detection of protein covalent binding and identification of reactive intermediates through analysis of glutathione adducts.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
The presence of high levels, as well as tissue-specific forms, of cytochrome P450 enzymes in mammalian olfactory mucosa (OM) has important implications in the bioactivation and toxicity of xenobiotics entering the tissue. Previous studies have shown that coumarin, a known olfactory toxicant in rats, is bioactivated by OM microsomal P450s to a number of products, presumably via coumarin-3,4-epoxide and other epoxide intermediates. The aim of the current study was to obtain direct evidence for the formation of such reactive intermediates in rat OM through the detection of protein covalent binding and glutathione (GSH) ad...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Zhuo X, Zhao W, Zheng J, Humphreys WG, Shu YZ, Zhu M Tags: Chem Biol Interact Source Type: journals

Manipulations of metallothionein gene dose accelerate the response to Listeria monocytogenes.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Metallothioneins (MTs) are cysteine-rich proteins that assist in cellular homeostasis and protect against oxidant injury. MTs can be induced by heavy metals and inflammatory mediators and function as free radical scavengers, reservoirs for essential heavy metals, and immunomodulators. In light of MTs' roles in responses to stress, we evaluated the in vivo effects of MT gene dose on the course of Listeria monocytogenes (LM) infection. LM burden was measured in livers and spleens, and flow cytometric assays were used to analyze splenocyte surface sulfhydryls, oxidative burst and apoptosis. Our results suggest that deviat...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Emeny RT, Marusov G, Lawrence DA, Pederson-Lane J, Yin X, Lynes MA Tags: Chem Biol Interact Source Type: journals

Gallic acid ester derivatives induce apoptosis and cell adhesion inhibition in melanoma cells: The relationship between free radical generation, glutathione depletion and cell death.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Malignant melanoma is a lethal disease, and the incidence and mortality associated with it are increasing worldwide. It has a significant tendency to develop both metastasis and resistance to chemotherapy. The tumor cells show abnormal redox regulation, and although the molecular mechanisms involved are not well characterized, they seem to be related to oxidative stress. In a previous study, we showed the antitumoral properties of gallic acid ester derivatives in leukemia cells. Here, we show the effect of octyl, decyl, dodecyl and tetradecyl gallates on B16F10 cells, a melanoma cell line. All compounds induced cytotox...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Locatelli C, Leal PC, Yunes RA, Nunes RJ, Creczynski-Pasa TB Tags: Chem Biol Interact Source Type: journals

Sodium arsenite-induced DAPK promoter hypermethylation and autophagy via ERK1/2 phosphorylation in human uroepithelial cells.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Arsenic compounds or arsenicals are well-known toxic and carcinogenic agents. The toxic effects of arsenic that are of most concern to humans are those that occur from chronic, low-level exposure, and are associated with various human malignancies, including skin, lung and bladder cancers. In addition, arsenic could induce cell death, including apoptosis or autophagy in malignant cells. Previously, we have demonstrated that arsenite can induce autophagy and death-associated protein kinase (DAPK) promoter hypermethylation in the SV-40 immortalized human uroepithelial cell line (SV-HUC-1). However, the underlying mechani...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Huang YC, Hung WC, Chen WT, Yu HS, Chai CY Tags: Chem Biol Interact Source Type: journals

Operculina turpethum attenuates N-nitrosodimethylamine induced toxic liver injury and clastogenicity in rats.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
The root extract of Operculina turpethum (OTE) has been used as an anti-inflammatory, purgative, and hepato-protective agent. N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. In the present study, we examined the therapeutic effects of OTE root extract against NDMA-induced hepatotoxicity and clastogenicity in rats. Hepatic fibrosis was induced in adult male albino rats through serial intraperitoneal administrations of NDMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. A group of rats received OTE orally in doses of...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Ahmad R, Ahmed S, Khan NU, Hasnain AU Tags: Chem Biol Interact Source Type: journals

Acrolein induces a cellular stress response and triggers mitochondrial apoptosis in A549 cells.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
This study investigates the activation of antiapoptosis survival factors in relation to the induction of cell death by apoptosis, following exposure to low doses of acrolein, in A549 human lung cells. Exposure to acrolein (<15microM, 30min) activated the survival factor AKT, which led to phosphorylation of Bad and induction of antiapoptosis proteins cIAP1/2. Acrolein (10-50microM, 30-60min) increased reactive oxygen species and caused mitochondrial membrane hyperpolarisation. Inhibition by the antioxidants catalase, polyethylene glycol-catalase, sodium pyruvate and MnTBAP showed that acrolein-induced reactive oxygen spe...
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Roy J, Pallepati P, Bettaieb A, Tanel A, Averill-Bates DA Tags: Chem Biol Interact Source Type: journals

Suillin from the mushroom Suillus placidus as potent apoptosis inducer in human hepatoma HepG2 cells.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
During the search of new anti-cancer agent from high fungi, the ethyl acetate extract of the mushroom Suillus placidus was found to exhibit a significant cytotoxic activity against human hepatoma HepG2 cells. With bioassay-guided fractionation, a cytotoxic component suillin was isolated from the extract. The anti-cancer effect of suillin was subsequently examined in 8 human cancer cell lines by using MTT assay. It is of interest to note that human liver cancer cells (HepG2 cells, Hep3B cells, and SK-Hep-1) were preferentially killed by suillin with an IC(50) of approximately 2microM in a 48h treatment. Mechanistically....
Source: Chemico-Biological Interactions - September 5, 2009 Category: Molecular Biology Authors: Liu FY, Luo KW, Yu ZM, Co NN, Wu SH, Wu P, Fung KP, Kwok TT Tags: Chem Biol Interact Source Type: journals

Modulation of the immune response by probiotic strains in a mouse model of gluten sensitivity.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
In this study, we explored the effect of different Lactobacillus spp. and Bifidobacterium lactis in transgenic mice expressing the human DQ8 heterodimer, a HLA molecule linked to Celiac Disease (CD). In vitro analysis on immature bone marrow-derived dendritic cells (iBMDCs) showed that all strains up-regulated surface B7-2 (CD86), indicative of DC maturation, however, with different intensity. No strain induced appreciable levels of IL-10 or IL-12 in iBMDCs, whereas TNF-alpha expression was essentially elicited by Lactobacillus paracasei and Lactobacillus fermentum. Interestingly, these strains were found also to increase ...
Source: Cytokine - September 4, 2009 Category: Molecular Biology Authors: D'Arienzo R, Maurano F, Lavermicocca P, Ricca E, Rossi M Tags: Cytokine Source Type: journals

Detection of site-specific glycosylation in proteins using flow cytometryemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
We tested the possibility that we may express unique peptide probes on cell surfaces, and detect site-specific glycosylation on these peptides using flow cytometry. Such development can enhance the application of flow cytometry to detect and quantify post-translational modifications in proteins. To this end, the N-terminal section of the human leukocyte glycoprotein PSGL-1 (P-selectin glycoprotein ligand-1) was modified to contain a poly-histidine tag followed by a proteolytic cleavage site. Amino acids preceding the cleavage site have a single O-linked glycosylation site. The recombinant protein called PSGL-1 (HT) was exp...
Source: Cytometry Part A - September 3, 2009 Category: Molecular Biology Authors: Deepak Jayakumar, Dhananjay D. Marathe, Sriram Neelamegham Source Type: journals

Alternate dab-aegPNAs: synthesis, nucleic acid binding studies and biological activityemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Giovanni N. Roviello, Domenica Musumeci, Andrea De Cristofaro, Domenica Capasso, Sonia Di Gaetano, Enrico M. Bucci, Carlo Pedone (Paper from Mol. BioSyst.) Giovanni N. Roviello, Mol. BioSyst., 2009, DOI: 10.1039/b910278g To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 3, 2009 Category: Molecular Biology Authors: Giovanni N. RovielloDomenica Musumeci Andrea De Cristofaro Domenica Capasso Sonia Di Gaetano Enrico M. Bucci Carlo Pedone Source Type: journals

Ligand dependent intra and inter subunit communication in human tryptophanyl tRNA synthetase as deduced from the dynamics of structure networksemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Priti Hansia, Amit Ghosh, Saraswathi Vishveshwara (Paper from Mol. BioSyst.) Priti Hansia, Mol. BioSyst., 2009, DOI: 10.1039/b903807h To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 3, 2009 Category: Molecular Biology Authors: Priti HansiaAmit Ghosh Saraswathi Vishveshwara Source Type: journals

Metabolic profiling of the fission yeast S. pombe: quantification of compounds under different temperatures and genetic perturbationemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Tomas Pluskal, Takahiro Nakamura, Alejandro Villar-Briones, Mitsuhiro Yanagida (Paper from Mol. BioSyst.) Tomas Pluskal, Mol. BioSyst., 2009, DOI: 10.1039/b908784b To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 3, 2009 Category: Molecular Biology Authors: Tomas PluskalTakahiro Nakamura Alejandro Villar-Briones Mitsuhiro Yanagida Source Type: journals

Methodological considerations in the development of HPLC-MS methods for the analysis of rodent plasma for metabonomic studiesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Lindsay Lai, Filippos Michopoulos, Helen Gika, Georgios Theodoridis, Robert W. Wilkinson, Rajesh Odedra, Julie Wingate, Ron Bonner, Stephen Tate, Ian D. Wilson (Method from Mol. BioSyst.) Lindsay Lai, Mol. BioSyst., 2009, DOI: 10.1039/b910482h To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 3, 2009 Category: Molecular Biology Authors: Lindsay LaiFilippos Michopoulos Helen Gika Georgios Theodoridis Robert W. Wilkinson Rajesh Odedra Julie Wingate Ron Bonner Stephen Tate Ian D. Wilson Source Type: journals

Structure and organization of drug-target networks: insights from genomic approaches for drug discoveryemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Sarath Chandra Janga, Andreas Tzakos (Review from Mol. BioSyst.) Sarath Chandra Janga, Mol. BioSyst., 2009, DOI: 10.1039/b908147j To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 3, 2009 Category: Molecular Biology Authors: Sarath Chandra JangaAndreas Tzakos Source Type: journals

Incorporation of unnatural amino acids for synthetic biologyemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Natalya Voloshchuk, Jin Kim Montclare (Review from Mol. BioSyst.) Natalya Voloshchuk, Mol. BioSyst., 2009, DOI: 10.1039/b909200p To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - September 3, 2009 Category: Molecular Biology Authors: Natalya VoloshchukJin Kim Montclare Source Type: journals

Multicatalytic tandem reaction of N[prime or minute]-(2-alkynylbenzylidene)hydrazide with indoleemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Xingxin Yu, Xiaodi Yang, Jie Wu (Paper from Org. Biomol. Chem.) Xingxin Yu, Org. Biomol. Chem., 2009, DOI: 10.1039/b913409c To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - September 3, 2009 Category: Molecular Biology Authors: Xingxin YuXiaodi Yang Jie Wu Source Type: journals

Quaternary history and contemporary patterns in a currently expanding speciesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: In regions that were not prone to marked glaciations, recent moth introductions/expansions could be detected due to the existence of a strong spatial genetic structure. In contrast, in regions that experienced the most intense Quaternary climatic oscillations, the natural populations are not genetically structured, and contemporary patterns of population expansion remain undetected.
Source: BMC Evolutionary Biology - Latest articles - September 3, 2009 Category: Molecular Biology Authors: Carole KerdelhueLorenzo ZaneMauro SimonatoPaola SalvatoJerome RousseletAlain RoquesAndrea Battisti Source Type: journals

Promoter analysis of the rabbit POU5F1 gene and its expression in preimplantation stage embryosemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: In this study we provide a comparative sequence analysis of the regulatory region of rabbit POU5F1 gene. Our data suggest that the POU5F1 gene is strictly regulated during early mammalian development. We proposed that the well conserved CR4 region containing the DE-2A enhancer is responsible for the highly conserved ESC specific gene expression. Notably, we are the first to report that the rabbit POU5F1 is not restricted to ICM cells only, but it is expressed in trophoblast cells as well. This information may be well applicable to investigate further the possible phylogenetic role and the regulation of POU5F1 gene.
Source: BMC Molecular Biology - Latest articles - September 3, 2009 Category: Molecular Biology Authors: Julianna KobolakKatalin KissZsuzsanna PolgarSolomon MamoClaire Rogel-GaillardZsuzsanna TancosIstvan BockArpad BajiKrisztina TarMelinda PirityAndras Dinnyes Source Type: journals

The crosstalk between EGF, IGF, and Insulin cell signaling pathways - computational and experimental analysisemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The simple model implemented in this paper provides a valuable first step in modeling signaling networks. However, to obtain a fully predictive model, a more detailed knowledge regarding parameters of individual interactions might be necessary.
Source: BMC Systems Biology - Latest articles - September 3, 2009 Category: Molecular Biology Authors: Rafal ZielinskiPawel PrzytyckiJie ZhengDavid ZhangTeresa PrzytyckaJacek Capala Source Type: journals

The genotype-phenotype relationship in multicellular pattern-generating models - the neglected role of pattern descriptorsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The results suggest that our approach may qualify as a general procedure for how to relate relevant features and characteristics of emergent patterns to the functional relationships, parameter values and initial values of an underlying pattern-generating mathematical model.
Source: BMC Systems Biology - Latest articles - September 3, 2009 Category: Molecular Biology Authors: Harald MartensSiren VeflingstadErik PlahteMagni MartensDominique BertrandStig Omholt Source Type: journals

Short-term synaptic plasticity in the nociceptive thalamic-anterior cingulate pathwayemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: The potentiation of ACC neuronal activity induced by thalamic bursting suggest that short-term synaptic plasticities enable the processing of nociceptive information from the medial thalamus and this temporal response variability is particularly important in pain because temporal maintenance of the response supports cortical integration and memory formation related to noxious events. Moreover, these modifications of cingulate synapses appear to regulate afferent signals that may be important to the transition from acute to chronic pain conditions associated with persistent peripheral noxious stimulation. Enhanc...
Source: Molecular Pain - September 3, 2009 Category: Molecular Biology Authors: Bai Chuang ShyuBrent Vogt Source Type: journals