Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 3.
A novel N-terminal motif of dipeptidyl peptidase-like proteins produces rapid inactivation of K(V)4.2 channels by a pore-blocking mechanism.
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The somatodendritic subthreshold A-type K(+) current in neurons (I(SA)) depends on its kinetic and voltage-dependent properties to regulate membrane excitability, action potential repetitive firing, and signal integration. Key functional properties of the K(V)4 channel complex underlying I(SA) are determined by dipeptidyl peptidase-like proteins known as dipeptidyl peptidase 6 (DPP6) and dipeptidyl peptidase 10 (DPP10). Among the multiple known DPP10 isoforms with alternative N-terminal sequences, DPP10a confers exceptionally fast inactivation to K(V)4.2 channels. To elucidate the molecular basis of this fast inactivat...
Source: Channels - November 12, 2009 Category: Molecular Biology Authors: Jerng HH, Dougherty K, Covarrubias M, Pfaffinger PJ Tags: Channels (Austin) Source Type: journals
In Memoriam: Tony J. Beugelsdijk (1949--2009)Email this article to a colleague.
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Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Tags: Articles Source Type: journals
Automated High-Content Screening for Compounds That Disassemble the Perinucleolar CompartmentEmail this article to a colleague.
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All solid malignancies share characteristic traits, including unlimited cellular proliferation, evasion of immune regulation, and the propensity to metastasize. The authors have previously described that a subnuclear structure, the perinucleolar compartment (PNC), is associated with the metastatic phenotype in solid tumor cancer cells. The percentage of cancer cells that contain PNCs (PNC prevalence) is indicative of the malignancy of a tumor both in vitro and in vivo, and thus PNC prevalence is a marker that reflects metastatic capability in a population of tumor cells. Although the function of the PNC remains to be deter...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Norton, J. T., Titus, S. A., Dexter, D., Austin, C. P., Zheng, W., Huang, S. Tags: Articles Source Type: journals
Profiling Bioactivity of the ToxCast Chemical Library Using BioMAP Primary Human Cell SystemsEmail this article to a colleague.
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The complexity of human biology has made prediction of health effects as a consequence of exposure to environmental chemicals especially challenging. Complex cell systems, such as the Biologically Multiplexed Activity Profiling (BioMAP) primary, human, cell-based disease models, leverage cellular regulatory networks to detect and distinguish chemicals with a broad range of target mechanisms and biological processes relevant to human toxicity. Here the authors use the BioMAP human cell systems to characterize effects relevant to human tissue and inflammatory disease biology following exposure to the 320 environmental chemic...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Houck, K. A., Dix, D. J., Judson, R. S., Kavlock, R. J., Yang, J., Berg, E. L. Tags: Articles Source Type: journals
C5a-Stimulated Recruitment of {beta}-Arrestin2 to the Nonsignaling 7-Transmembrane Decoy Receptor C5L2Email this article to a colleague.
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C5L2 (or GPR77) is a high-affinity receptor for the complement fragment C5a and its desarginated product, C5a-desArg. Unlike the classical C5a receptor CD88, C5L2 does not couple to intracellular G-protein-signaling pathways but is thought to function as a decoy receptor. The authors show that stimulation of C5L2 with C5a and C5a-desArg induces redistribution of green fluorescent protein—labeled β-arrestin2 to cytoplasmic vesicles. C3a and C3a-desArg were inactive in the β-arrestin translocation assay. Direct interaction of ligand-stimulated C5L2 with β-arrestin was confirmed using a novel β-galac...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Van Lith, L. H.C., Oosterom, J., Van Elsas, A., Zaman, G. J.R. Tags: Articles Source Type: journals
CXCR2 Inverse Agonism Detected by Arrestin RedistributionEmail this article to a colleague.
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To study CXCR2 modulated arrestin redistribution, the authors employed arrestin as a fusion protein containing either the Aequorea victoria—derived enhanced green fluorescent protein (EGFP) or a recently developed mutant of eqFP611, a red fluorescent protein derived from Entacmaea quadricolor. This mutant, referred to as RFP611, had earlier been found to assume a dimeric quarternary structure. It was therefore employed in this work as a "tandem" (td) construct for pseudo monomeric fusion protein labeling. Both arrestin fusion proteins, containing either td RFP611 (Arr td RFP611) or enhanced green fluorescent protein ...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Kredel, S., Wolff, M., Wiedenmann, J., Moepps, B., Nienhaus, G. U., Gierschik, P., Kistler, B., Heilker, R. Tags: Articles Source Type: journals
Inhibitors of RecA Activity Discovered by High-Throughput Screening: Cell-Permeable Small Molecules Attenuate the SOS Response in Escherichia coliEmail this article to a colleague.
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The phenomenon of antibiotic resistance has created a need for the development of novel antibiotic classes with nonclassical cellular targets. Unfortunately, target-based drug discovery against proteins considered essential for in vitro bacterial viability has yielded few new therapeutic classes of antibiotics. Targeting the large proportion of genes considered nonessential that have yet to be explored by high-throughput screening, for example, RecA, can complement these efforts. Recent evidence suggests that RecA-controlled processes are responsible for tolerance to antibiotic chemotherapy and are involved in pathways tha...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Wigle, T. J., Sexton, J. Z., Gromova, A. V., Hadimani, M. B., Hughes, M. A., Smith, G. R., Yeh, L.-A., Singleton, S. F. Tags: Articles Source Type: journals
Optimization of Assay Conditions fo r Dengue Virus Protease: Effect of Various Polyols and Nonionic DetergentsEmail this article to a colleague.
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The aim of this work was to perform a systematic study of the effect of nonionic detergents on the activity of the dengue virus NS2B-NS3 protease. To ensure a high activity of the protease, the assay procedures for the dengue virus and other flaviviral proteases published to date are performed in the presence of up to 35% glycerol, which does not represent the cellular physicochemical environment. In addition, the high viscosity of glycerol-containing solutions leads to various experimental problems in miniaturized assays. Using an internally quenched peptide substrate, the authors show that glycerol is not essential for e...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Steuer, C., Heinonen, K. H., Kattner, L., Klein, C. D. Tags: Articles Source Type: journals
Virtual Screening Against {alpha}-CobratoxinEmail this article to a colleague.
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-Cobratoxin (Cbtx), the neurotoxin isolated from the venom of the Thai cobra Naja kaouthia , causes paralysis by preventing acetylcholine (ACh) binding to nicotinic acetylcholine receptors (nAChRs). In the current study, the region of the Cbtx molecule that is directly involved in binding to nAChRs is used as the target for anticobratoxin drug design. The crystal structure (1YI5) of Cbtx in complex with the acetylcholine binding protein (AChBP), a soluble homolog of the extracellular binding domain of nAChRs, was selected to prepare an -cobratoxin active binding site for docking. The amino acid residues (Ser182-Tyr192) of ...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Utsintong, M., Talley, T. T., Taylor, P. W., Olson, A. J., Vajragupta, O. Tags: Articles Source Type: journals
A Novel High-Throughput Screening Assay for HCN Channel Blocker Using Membrane Potential-Sensitive Dye and FLIPREmail this article to a colleague.
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In this study, the authors report the development of a novel membrane potential-sensitive dye (MPSD) assay for HCN channel modulators that has been miniaturized into 384-well fluorescent imaging plate reader (FLIPR) high-throughput screening (HTS) format. When optimized (by cell plating density, plate type, cell recovery from cryopreservation), the wellto-well signal variability was low, with a Z' = 0.73 and coefficient of variation = 6.4%, whereas the MPSD fluorescence signal amplitude was -23,700 ± 1500 FLIPR3 relative fluorescence units (a linear relationship was found between HCN1 MPSD fluorescence signal and th...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Vasilyev, D. V., Shan, Q. J., Lee, Y. T., Soloveva, V., Nawoschik, S. P., Kaftan, E. J., Dunlop, J., Mayer, S. C., Bowlby, M. R. Tags: Articles Source Type: journals
A Continuous Protein Methyltransferase (G9a) Assay for Enzyme Activity Measurement and Inhibitor ScreeningEmail this article to a colleague.
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The authors describe a continuous protein methylation assay using the G9a protein lysine methyltransferase and its substrate protein WIZ (widely interspaced zinc finger motifs). The assay is based on the coupling of the biotinylated substrate protein to streptavidin-coated FlashPlates and the transfer of radioactive methyl groups from the S-adenosyl-L-methionine to the substrate. The reaction progress is monitored continuously by proximity scintillation counting. The assay is very accurate, convenient, well suited for automation, and highly reproducible with standard errors in the range of 5%. Because of few pipetting step...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Dhayalan, A., Dimitrova, E., Rathert, P., Jeltsch, A. Tags: Articles Source Type: journals
Utilization of the TangoTM {beta}-Arrestin Recruitment Technology for Cell-Based EDG Receptor Assay Development and InterrogationEmail this article to a colleague.
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Cellular assay development for the endothelial differentiation gene (EDG) family of G-protein-coupled receptors (GPCRs) and related lysophospholipid (LP) receptors is complicated by endogenous receptor expression and divergent receptor signaling. Endogenously expressed LP receptors exist in most tissue culture cell lines. these LP receptors, along with other endogenously expressed GPCRs, contribute to off-target signaling that can complicate interpretation of second-messenger-based cellular assay results. these receptors also activate a diverse and divergent set of cellular signaling pathways, necessitating the use of a va...
Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Authors: Wetter, J. A., Revankar, C., Hanson, B. J. Tags: Articles Source Type: journals
Product Focus: Analytical and Preparative InstrumentationEmail this article to a colleague.
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Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Tags: Articles Source Type: journals
2009 Meetings CalendarEmail this article to a colleague.
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Source: Journal of Biomolecular Screening - November 12, 2009 Category: Molecular Biology Tags: Articles Source Type: journals
Anticancer activities of artemisinin and its bioactive derivativesEmail this article to a colleague.
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Review ArticlesGary L. Firestone, Shyam N. Sundar, Expert Reviews in Molecular Medicine, Volume 11 , pp e32AbstractArtemisinin, a sesquiterpene lactone derived from the sweet wormwood plant Artemisia annua, and its bioactive derivatives exhibit potent anticancer effects in a variety of human cancer cell model systems. The pleiotropic response in cancer cells includes growth inhibition by cell cycle arrest, apoptosis, inhibition of angiogenesis, disruption of cell migration, and modulation of nuclear receptor responsiveness. These effects of artemisinin and its derivatives result from perturbations of many cellular signalli...
Source: Expert Reviews in Molecular Medicine - November 12, 2009 Category: Molecular Biology Source Type: journals
Disruption of Tsc2 in oocytes leads to overactivation of the entire pool of primordial folliclesEmail this article to a colleague.
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In this study, we provide some genetic evidence to show that the tumor suppressor tuberous sclerosis complex 2 (Tsc2), which negatively regulates mammalian target of rapamycin complex 1 (mTORC1), functions in oocytes to maintain the dormancy of primordial follicles. In mutant mice lacking the Tsc2 gene in oocytes, the pool of primordial follicles is activated prematurely due to elevated mTORC1 activity in oocytes. This results in depletion of follicles in early adulthood, causing premature ovarian failure (POF). Our results suggest that the Tsc1–Tsc2 complex mediated suppression of mTORC1 activity is indispensable fo...
Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Adhikari, D., Flohr, G., Gorre, N., Shen, Y., Yang, H., Lundin, E., Lan, Z., Gambello, M. J., Liu, K. Tags: Original Articles Source Type: journals
The forkhead transcription factor FOXL2 is expressed in somatic cells of the human ovary prior to follicle formationEmail this article to a colleague.
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Interactions between germ cells and surrounding somatic cells are central to ovarian development as well as later function. Disruption of these interactions arising from abnormalities in either cell type can lead to premature ovarian failure (POF). The forkhead transcription factor FOXL2 is a candidate POF factor, and mutations in the FOXL2 gene are associated with syndromic and non-syndromic ovarian failure. Foxl2-deficient mice display major defects in primordial follicle activation with consequent follicle loss, and earlier roles in gonadal development and sex determination have also been suggested. However, despite its...
Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Duffin, K., Bayne, R.A.L., Childs, A.J., Collins, C., Anderson, R.A. Tags: Original Articles Source Type: journals
Stable expression and characterization of N-terminal tagged recombinant human bone morphogenetic protein 15Email this article to a colleague.
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Oocyte-derived growth factors are critically involved in multiple ovarian processes via paracrine actions. Although recombinant proteins have been applied to dissect the physiological functions of these factors, variation of activities among different protein preparations remains an issue. To further elucidate the roles of one of these growth factors, bone morphogenetic protein 15 (BMP15), in mediating oocyte-regulated molecular and cellular events and to explore its potential clinical application, we engineered the human BMP15 sequence to efficiently produce bioactive recombinant human BMP15 (rhBMP15). The proteolytic cle...
Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Li, Q., Rajanahally, S., Edson, M. A., Matzuk, M. M. Tags: Original Articles Source Type: journals
Oocyte peptides as paracrine tools for ovarian stimulation and oocyte maturationEmail this article to a colleague.
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Recent studies report the production and isolation of a stable bioactive recombinant human bone morphogenetic protein 15 (rhBMP15) that is appropriately processed in HEK-293 cells and activates the SMAD 1/5/8 pathway in mouse granulosa cell cultures. Further, the purified rhBMP15 induces the expression of genes associated with cumulus expansion. Thanks to recent research, we have a greater understanding of the importance of the dialogue that occurs between the oocyte and the granulosa cell layer with regard to regulating folliculogenesis and the acquisition of oocyte developmental competence and maturation. BMP15 is one of...
Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Mottershead, D. G., Watson, A. J. Tags: Commentary Source Type: journals
The primordial pool of follicles and nest breakdown in mammalian ovariesEmail this article to a colleague.
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The creation of the pool of follicles available for selection and ovulation is a multi-faceted, tightly regulated process that spans the period from embryonic development through to the first reproductive cycle of the organism. In mice, this development can occur in mere weeks, but in humans, it is sustained for years. Embryonic germ cell development involves the migration of primordial germs cells to the genital ridge, and the mitotic division of germ cell nuclei without complete cytokinesis to form a multi-nucleated syncytia, or germ cell nest. Through combined actions of germ cell apoptosis and somatic cell migration, t...
Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Tingen, C., Kim, A., Woodruff, T. K. Tags: New Research Horizon Reviews Source Type: journals
Oogenesis and cell death in human prenatal ovaries: what are the criteria for oocyte selection?Email this article to a colleague.
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Prenatal oogenesis produces hundreds of thousands of oocytes, most of which are discarded through apoptosis before birth. Despite this large-scale selection, the survivors do not constitute a perfect population, and the factors at the cellular level that result in apoptosis or survival of any individual oocyte are largely unknown. What then are the selection criteria that determine the size and quality of the ovarian reserve in women? This review focuses on new data at the cellular level, on human prenatal oogenesis, offering clues about the importance of the timing of entry to meiotic prophase I by linking the stages and ...
Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Hartshorne, G.M., Lyrakou, S., Hamoda, H., Oloto, E., Ghafari, F. Tags: New Research Horizon Reviews Source Type: journals
Control of ovulation in mice by progesterone receptor-regulated gene networksEmail this article to a colleague.
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The mid-cycle surge of luteinizing hormone (LH) induces ovulation, a process during which a fertilizable oocyte is released from a mature ovarian follicle. Although ovulation is a physiologically well-characterized event, the underlying molecular pathways remain poorly understood. Progesterone receptor (PGR), which mediates the biological effects of the steroid hormone progesterone, has emerged as a key regulator of ovulation in mice. The development of a progesterone-receptor-null (Pgr-null) mouse model confirmed a critical role of this hormone in ovulation because in these mutant mice, mature pre-ovulatory follicles fail...
Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Kim, J., Bagchi, I. C., Bagchi, M. K. Tags: New Research Horizon Reviews Source Type: journals
Genetic and gene expression analyses of the polycystic ovary syndrome candidate gene fibrillin-3 and other fibrillin family members in human ovariesEmail this article to a colleague.
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Several studies have demonstrated an association between polycystic ovary syndrome (PCOS) and the dinucleotide repeat microsatellite marker D19S884, which is located in intron 55 of the fibrillin-3 (FBN3) gene. Fibrillins, including FBN1 and 2, interact with latent transforming growth factor (TGF)-β-binding proteins (LTBP) and thereby control the bioactivity of TGFβs. TGFβs stimulate fibroblast replication and collagen production. The PCOS ovarian phenotype includes increased stromal collagen and expansion of the ovarian cortex, features feasibly influenced by abnormal fibrillin expression. To examine a poss...
Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Prodoehl, M. J., Hatzirodos, N., Irving-Rodgers, H. F., Zhao, Z. Z., Painter, J. N., Hickey, T. E., Gibson, M. A., Rainey, W. E., Carr, B. R., Mason, H. D., Norman, R. J., Montgomery, G. W., Rodgers, R. J. Tags: Original Article Source Type: journals
Paracrine support of ovarian stimulationEmail this article to a colleague.
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Assisted reproductive technology has evolved on the back of blunderbuss ovarian stimulation regimes designed to maximize the number of oocytes recoverable for treatment purposes. However, oocyte ‘quality’ is finely programmed by local paracrine and autocrine signalling events during folliculogenesis and can be adversely affected by inappropriate gonadotrophic stimulation. This brief review traces the full follicular lifespan—from initiation to ovulation—to identify gonadotrophin-responsive checkpoints likely to impact oocyte quality. It is argued that these might be targeted during controlled ovaria...
Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Hillier, S. G. Tags: New Research Horizon Reviews Source Type: journals
The ovary: from basic research to clinicEmail this article to a colleague.
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Source: Molecular Human Reproduction - November 12, 2009 Category: Molecular Biology Authors: Hillier, S. G. Tags: Editorial Source Type: journals
Up regulation of GW112 Gene by NF[kappa]B promotes an antiapoptotic property in gastric cancer cellsEmail this article to a colleague.
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To clarify the regulatory mechanism of GW112 gene expression, 5[prime]-flanking region of the human GW112 gene was isolated and characterized in the present study. 5[prime]-RACE analysis showed a single transcription start site, which is located 142 nucleotides upstream of the translation initiation site. Transient transfection studies with serial deletion constructs and close examination of the sequences identified a putative NF[kappa]B binding sequence between -442 and -430, which could be responsible for efficient expression of the GW112 gene. Indeed, GW112 gene was found to be regulated by NF[kappa]B signals including ...
Source: Molecular Carcinogenesis - November 12, 2009 Category: Molecular Biology Authors: Kee K. Kim, Key S. Park, Seok B. Song, Kyoon E. Kim Source Type: journals
Three-dimensional polar representation for multispectral fluorescence lifetime imaging microscopyEmail this article to a colleague.
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We present here a new approach that considerably simplifies this analysis avoiding complex fitting algorithm strategies and permitting a fast and visual graphical representation of the fluorescence lifetimes. By transforming the experimental data from time domain to frequency domain for each spectral channel, we calculate the multispectral polar representation and demonstrate its interest on multiply fluorescent labeled sample. We further apply it on Förster resonance energy transfer (FRET) experiments and demonstrate that FRET measurements with a high level of precision can be performed. With addition of emission wavelen...
Source: Cytometry Part A - November 12, 2009 Category: Molecular Biology Authors: A. Leray, C. Spriet, D. Trinel, Laurent Héliot Source Type: journals
Therapeutic applications of hyaluronanEmail this article to a colleague.
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John Gaffney, Sabine Matou-Nasri, Marta Grau-Olivares, Mark Slevin
(Review from Mol. BioSyst.)
John Gaffney, Mol. BioSyst., 2010, DOI: 10.1039/b910552m
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Source: RSC - Mol. BioSyst. latest articles - November 12, 2009 Category: Molecular Biology Authors: John GaffneySabine Matou-Nasri Marta Grau-Olivares Mark Slevin Source Type: journals
Combining nanotechnology with current biomedical knowledge for the vascular imaging and treatment of atherosclerosisEmail this article to a colleague.
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M. Slevin, L. Badimon, M. Grau-Olivares, M. Ramis, J. Sendra, M. Morrison, J. Krupinski
(Review from Mol. BioSyst.)
M. Slevin, Mol. BioSyst., 2010, DOI: 10.1039/b916175a
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Source: RSC - Mol. BioSyst. latest articles - November 12, 2009 Category: Molecular Biology Authors: M. SlevinL. Badimon M. Grau-Olivares M. Ramis J. Sendra M. Morrison J. Krupinski Source Type: journals
Identification and biosynthesis of tropone derivatives and sulfur volatiles produced by bacteria of the marine Roseobacter cladeEmail this article to a colleague.
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Verena Thiel, Thorsten Brinkhoff, Jeroen S. Dickschat, Susanne Wickel, Jorg Grunenberg, Irene Wagner-Dobler, Meinhard Simon, Stefan Schulz
(Paper from Org. Biomol. Chem.)
Verena Thiel, Org. Biomol. Chem., 2010, DOI: 10.1039/b909133e
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Source: RSC - Organic Biomolecular Chemistry - November 12, 2009 Category: Molecular Biology Authors: Verena ThielThorsten Brinkhoff Jeroen S. Dickschat Susanne Wickel Jorg Grunenberg Irene Wagner-Dobler Meinhard Simon Stefan Schulz Source Type: journals
Stereoselective synthesis and structure-affinity relationships of bicyclic [small kappa] receptor agonistsEmail this article to a colleague.
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Daniel Kracht, Elisabeth Rack, Dirk Schepmann, Roland Frohlich, Bernhard Wunsch
(Paper from Org. Biomol. Chem.)
Daniel Kracht, Org. Biomol. Chem., 2010, DOI: 10.1039/b915180j
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Source: RSC - Organic Biomolecular Chemistry - November 12, 2009 Category: Molecular Biology Authors: Daniel KrachtElisabeth Rack Dirk Schepmann Roland Frohlich Bernhard Wunsch Source Type: journals
Mechanism of chemical O-glycosylation: from early studies to recent discoveriesEmail this article to a colleague.
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Laurel K. Mydock, Alexei V. Demchenko
(Perspective from Org. Biomol. Chem.)
Laurel K. Mydock, Org. Biomol. Chem., 2010, DOI: 10.1039/b916088d
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Source: RSC - Organic Biomolecular Chemistry - November 12, 2009 Category: Molecular Biology Authors: Laurel K. MydockAlexei V. Demchenko Source Type: journals
DIBAL-H mediated triple and quadruple debenzylations of perbenzylated cyclodextrinsEmail this article to a colleague.
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Girish K. Rawal, Shikha Rani, Sandra Ward, Chang-Chun Ling
(Paper from Org. Biomol. Chem.)
Girish K. Rawal, Org. Biomol. Chem., 2010, DOI: 10.1039/b915450g
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Source: RSC - Organic Biomolecular Chemistry - November 12, 2009 Category: Molecular Biology Authors: Girish K. RawalShikha Rani Sandra Ward Chang-Chun Ling Source Type: journals
The structural and functional determinants of the Axin and Dishevelled DIX domainsEmail this article to a colleague.
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Conclusions:
This report provides the first explanation of the mutant phenotypes caused by non-synonymous substitutions in the Dishevelled and Axin DIX domain by correlating their presumed functional significance with molecular structure.
Source: BMC Structural Biology - Latest articles - November 12, 2009 Category: Molecular Biology Authors: Matthias EhebauerAlfonso Martinez Arias Source Type: journals
A small supernumerary marker chromosome present in a Turner syndrome patient not derived from X- or Y-chromosome: a case reportEmail this article to a colleague.
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Conclusion:
More comprehensive characterization of such sSMCT might identify them to be more frequent than only ~0.6% in Turner syndrome cases according to available data.
Source: Molecular Cytogenetics - November 12, 2009 Category: Molecular Biology Authors: Frenny ShethElisabeth EwersNadezda KosyakovaAnja WeiseJayesh ShethManisha DesaiJoris AndrieuxJoris VermeeschAhmed HamidMonika ZieglerThomas Liehr Source Type: journals
Antinociceptive action of oxytocin involves inhibition of potassium channel currents in lamina II neurons of the rat spinal cordEmail this article to a colleague.
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Conclusions:
This effect of OT on the firing profile of lamina II neurons is in good agreement with the antinociceptive and analgesic properties of OT described in vivo.
Source: Molecular Pain - November 12, 2009 Category: Molecular Biology Authors: Jean-Didier BretonPierrick PoisbeauPascal Darbon Source Type: journals
Inhibition of MAPK signaling by eNOS gene transfer improves ventricular remodeling after myocardial infarction through reduction of inflammation.Email this article to a colleague.
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Endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) may play an important role in attenuating cardiac remodeling and apoptosis after myocardial infarction. However, the anti-inflammation effects of eNOS in infarcted myocardium and the role of MAPK signaling in eNOS/NO mediated cardiac remodeling have not yet been elucidated. Adenovirus carrying Human eNOS gene was delivered locally into heart 4 days prior to induction of myocardial infarction (MI) by left anterior descending coronary artery ligation. Monocyte/macrophage infiltration was detected by ED-1 immunohistochemistry. Western blot was employed to exam...
Source: Molecular Biology Reports - November 12, 2009 Category: Molecular Biology Authors: Chen LL, Zhu TB, Yin H, Huang J, Wang LS, Cao KJ, Yang ZJ Tags: Mol Biol Rep Source Type: journals
Identification and expression of an elastase homologue in Branchiostoma belcheri with implications to the origin of vertebrate pancreas.Email this article to a colleague.
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In this study we demonstrate the presence of an elastase homologue, named BbElas, in Branchiostoma belcheri. The recombinant BbElas hydrolyses the elastase specific substrate N-succinyl-Ala-Ala-Ala p-nitroanilide, which can be inhibited by the serine proteinase inhibitor PMSF, the elastase-specific inhibitor elastatinal and the cysteine proteinase inhibitor PCMB. Phylogenetic analysis shows that BbElas represents the archetype of vertebrate elastases, hinting at the clues that the different isoforms of vertebrate elastases are originated from an ancestral gene like BbElas. Our results also suggest that the mid-gut in amphi...
Source: Molecular Biology Reports - November 12, 2009 Category: Molecular Biology Authors: Sun T, Zhang S, Ji G Tags: Mol Biol Rep Source Type: journals
Ensuring comparability of benzene exposure estimates across three nested case-control studies in the petroleum industry in support of a pooled epidemiological analysis.Email this article to a colleague.
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CONCLUSIONS: The revised exposure estimates will be used in the pooled analysis to examine the risk of haematopoietic cancers and benzene exposure. This exercise provided an important quality control check on the exposure estimates and identified similarly exposed Job Categories that could be grouped for risk assessment analyses.
PMID: 19914227 [PubMed - as supplied by publisher]
Source: Chemico-Biological Interactions - November 12, 2009 Category: Molecular Biology Authors: Glass DC, Armstrong TW, Pearlman ED, Verma DK, Schnatter AR, Rushton L Tags: Chem Biol Interact Source Type: journals
Dietary flavonoids suppress azoxymethane-induced colonic preneoplastic lesions in male C57BL/KsJ-db/db mice.Email this article to a colleague.
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Obesity is known to be a risk factor for colon carcinogenesis. Although there are several reports on the chemopreventive abilities of dietary flavonoids in chemically induced colon carcinogenesis, those have not been addressed in an obesity-associated carcinogenesis model. In the present study, the effects of 3 flavonoids (chrysin, quercetin and nobiletin) on modulation of the occurrence of putative preneoplastic lesions, aberrant crypt foci (ACF), and beta-catenin accumulated crypts (BCACs) in the development of colon cancer were determined in male db/db mice with obesity and diabetic phenotypes. Male db/db mice were ...
Source: Chemico-Biological Interactions - November 12, 2009 Category: Molecular Biology Authors: Miyamoto S, Yasui Y, Ohigashi H, Tanaka T, Murakami A Tags: Chem Biol Interact Source Type: journals
How much does benzene contribute to the overall burden of cancer due to occupation?Email this article to a colleague.
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PMID: 19914225 [PubMed - as supplied by publisher]
Source: Chemico-Biological Interactions - November 12, 2009 Category: Molecular Biology Authors: Rushton L, Brown TP, Cherrie J, Fortunato L, Van Tongeren M, Hutchings SJ Tags: Chem Biol Interact Source Type: journals
Expression of hepatic antioxidant genes in L-thyroxine-induced hyperthyroid rats: Regulation by vitamin E and curcumin.Email this article to a colleague.
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Earlier we have demonstrated that oral supplementation of vitamin E and curcumin alleviates hyperthyroidism-induced oxidative stress and distorted histoarchitecture in rat liver (Chem. Biol. Interact. 173:105-114, 2008). To delineate the underlying mechanism of protection, the present study was undertaken to investigate the regulatory role of vitamin E and curcumin on antioxidant gene (AOG) expression in hyperthyroid rat liver. Adult male rats were rendered hyperthyroid by administration of 0.0012% L-thyroxine in their drinking water, while vitamin E (200mg/Kg body weight) and curcumin (30mg/Kg body weight) were supple...
Source: Chemico-Biological Interactions - November 12, 2009 Category: Molecular Biology Authors: Subudhi U, Chainy GB Tags: Chem Biol Interact Source Type: journals
Actin Binding Protein-1 Interacts with WIP to Regulate Growth Factor-induced Dorsal Ruffle Formation.Email this article to a colleague.
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Monitoring Editor: Josephine C. Adams Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin binding protein-1 (mAbp1) is an actin binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of growth factor-induced actin-based structures has not been defined. Here we show that mAbp1 localizes to dorsal ruffles and is necessary for platelet-derived growth factor (PDGF)-mediated dorsal ruffle formation. Despite their structural similarity, we find that mAbp1 and cortactin have nonr...
Source: Mol Biol Cell - November 12, 2009 Category: Molecular Biology Authors: Cortesio CL, Perrin BJ, Bennin DA, Huttenlocher A Tags: Mol Biol Cell Source Type: journals
Mechanisms of Self-organization of Cortical Microtubules in Plants Revealed by Computational Simulations.Email this article to a colleague.
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Monitoring Editor: David G. Drubin Microtubules confined to the two-dimensional cortex of elongating plant cells must form a parallel yet dispersed array transverse to the elongation axis for proper cell wall expansion. Some of these microtubules exhibit free minus-ends, leading to migration at the cortex by hybrid treadmilling. Collisions between microtubules can result in plus-end entrainment ("zippering") or rapid depolymerization. Here, we present a computational model of cortical microtubule organization. We find that plus-end entrainment leads to self-organization of microtubules into parallel arrays, while catas...
Source: Mol Biol Cell - November 12, 2009 Category: Molecular Biology Authors: Allard JF, Wasteneys GO, Cytrynbaum EN Tags: Mol Biol Cell Source Type: journals
Centromeric Localization of Dispersed Pol III Genes in Fission Yeast.Email this article to a colleague.
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We present evidence that Pol III transcribed genes such as tRNA and 5S rRNA genes can localize to centromeres and contribute to a global genome organization. Furthermore, we find that ectopic insertion of Pol III genes into a nonPol III gene locus results in the centromeric localization of the locus. We show that the centromeric localization of Pol III genes is mediated by condensin, which interacts with the Pol III transcription machinery, and that transcription levels of the Pol III genes are negatively correlated with the centromeric localization of Pol III genes. This centromeric localization of Pol III genes initially...
Source: Mol Biol Cell - November 12, 2009 Category: Molecular Biology Authors: Iwasaki O, Tanaka A, Tanizawa H, Grewal SI, Noma KI Tags: Mol Biol Cell Source Type: journals
Mobility, Microtubule Nucleation and Structure of MTOCs in Multinucleated Hyphae of Ashbya gossypii.Email this article to a colleague.
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Monitoring Editor: Kerry S. Bloom We investigated the migration of multiple nuclei in hyphae of the filamentous fungus Ashbya gossypii. Three types of cytoplasmic microtubule (cMT)-dependent nuclear movements were characterized using live cell imaging: short range oscillations (up to 4.5 mum/min), rotations (up to 180 degrees in 30 s) and long-range nuclear bypassing (up to 9 mum/min). These movements were superimposed on a cMT-independent mode of nuclear migration, cotransport with the cytoplasmic stream. This latter mode is sufficient to support wild-type-like hyphal growth speeds. cMT-dependent nuclear movements wer...
Source: Mol Biol Cell - November 12, 2009 Category: Molecular Biology Authors: Lang C, Grava S, van den Hoorn T, Trimble R, Philippsen P, Jaspersen SL Tags: Mol Biol Cell Source Type: journals
A Novel JNK Binding Protein WDR62 Is Recruited to Stress Granules and Mediates a Non-classical JNK Activation.Email this article to a colleague.
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Monitoring Editor: Jonathan Chernoff The c-Jun N-terminal kinase (JNK) is part of a Mitogen-activated protein kinase (MAPK) signaling cascade. Scaffold proteins simultaneously associate with various components of the MAPK signaling pathway and play a role in signal transmission and regulation. Here we describe the identification of a novel scaffold JNK binding protein, WDR62, with no sequence homology to any of the known scaffold proteins. WDR62 is a ubiquitously expressed heat-sensitive 175 kDa protein that specifically associates with JNK but not with ERK and p38. Association between WDR62 and JNKs occurs in the abse...
Source: Mol Biol Cell - November 12, 2009 Category: Molecular Biology Authors: Wasserman T, Katsenelson K, Daniliuc S, Hasin T, Choder M, Aronheim A Tags: Mol Biol Cell Source Type: journals
Blockade of multiple but not single cytokines abrogates downregulation of angiotensin II type-I receptors and anticipates septic shock.Email this article to a colleague.
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In this prospective, randomized animal study, the role of proinflammatory cytokines in the pathogenesis sepsis-induced circulatory failure with downregulation of angiotensin-II-type-I-(AT(1))-receptors was investigated. Sepsis in wild-type mice and in mice with deficiencies for TNF-alpha, IL-1beta, IFN-gamma or IL-6 was induced by cecal ligation and puncture (CLP) and wild-type mice were injected with cytokines. Animals were treated with glucocorticoids or small interfering RNA (siRNA) targeting single or multiple cytokines or NF-kappaB. Vascular smooth muscle cells (VSMCs) were incubated with cytokines. CLP resulted i...
Source: Cytokine - November 12, 2009 Category: Molecular Biology Authors: Schmidt C, Höcherl K, Kurt B, Moritz S, Kurtz A, Bucher M Tags: Cytokine Source Type: journals
Pigment epithelium–derived factor down regulates hyperglycemia-induced apoptosis via PI3K/Akt activation in goat retinal pericytesEmail this article to a colleague.
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Abstract Pigment epithelium–derived factor (PEDF) is a well-known protease inhibitor for angiogenesis in the eye, suggesting that loss
of PEDF in eye is implicated in the pathogenesis of proliferative diabetic retinopathy. Since the role of PEDF in diabetic
retinopathy is unclear, the effect of PEDF on different types of cells constituting the blood vessel has to be checked. Here,
we have investigated the effects of PEDF under hyperglycemic conditions in retinal pericytes, isolated from goat’s eye and
used to analyze the signaling pathway involved. High glucose increased the apoptotic cell death and intr...
Source: Angiogenesis - November 11, 2009 Category: Molecular Biology Tags: Angiogenesis Source Type: journals
The role of two putative nitroreductases, Frm2p and Hbn1p, in the oxidative stress response in Saccharomyces cerevisiaeEmail this article to a colleague.
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The nitroreductase family is comprised of a group of FMN- or FAD-dependent enzymes that are able to metabolize nitrosubstituted compounds using the reducing power of NAD(P)H. These nitroreductases can be found in bacterial species and, to a lesser extent, in eukaryotes. There is little information on the biochemical functions of nitroreductases. Some studies suggest their possible involvement in the oxidative stress response. In the yeast Saccharomyces cerevisiae, two nitroreductase proteins, Frm2p and Hbn1p, have been described. While Frm2p appears to act in the lipid signalling pathway, the function of Hbn1p is completel...
Source: Yeast - November 11, 2009 Category: Molecular Biology Authors: Iuri Marques de Oliveira, Alfeu Zanotto-Filho, José Cláudio Fonseca Moreira, Diego Bonatto, João Antonio Pêgas Henriques Source Type: journals
