Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 5.
Synthesis and biological evaluation of phosphatidylinositol phosphate affinity probes
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Stuart J. Conway, James Gardiner, Simon J. A. Grove, Melloney K. Johns, Ze-Yi Lim, Gavin F. Painter, Diane E. J. E. Robinson, Christine Schieber, Jan W. Thuring, Leon S.-M. Wong, Meng-Xin Yin, Antony W. Burgess, Bruno Catimel, Phillip T. Hawkins, Nicholas T. Ktistakis, Leonard R. Stephens, Andrew B. Holmes
(Paper from Org. Biomol. Chem.)
Stuart J. Conway, Org. Biomol. Chem., 2010, DOI: 10.1039/b913399b
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The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - November 9, 2009 Category: Molecular Biology Authors: Stuart J. ConwayJames Gardiner Simon J. A. Grove Melloney K. Johns Ze-Yi Lim Gavin F. Painter Diane E. J. E. Robinson Christine Schieber Jan W. Thuring Leon S.-M. Wong Meng-Xin Yin Antony W. Burgess Bruno Catimel Phillip T. Hawkins Nicholas T. Ktistakis L Source Type: journals
An efficient and convenient Cu(OAc)2/air mediated oxidative coupling of azoles via C-H activationEmail this article to a colleague.
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Yan Li, Jun Jin, Weixing Qian, Weiliang Bao
(Communication from Org. Biomol. Chem.)
Yan Li, Org. Biomol. Chem., 2010, DOI: 10.1039/b919396k
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Source: RSC - Organic Biomolecular Chemistry - November 9, 2009 Category: Molecular Biology Authors: Yan LiJun Jin Weixing Qian Weiliang Bao Source Type: journals
Cyclooctyne-based reagents for uncatalyzed click chemistry: A computational surveyEmail this article to a colleague.
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Kimberly Chenoweth, David Chenoweth, William A. Goddard III
(Paper from Org. Biomol. Chem.)
Kimberly Chenoweth, Org. Biomol. Chem., 2009, DOI: 10.1039/b911482c
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Source: RSC - Organic Biomolecular Chemistry - November 9, 2009 Category: Molecular Biology Authors: Kimberly ChenowethDavid Chenoweth William A. Goddard III Source Type: journals
Small molecule induced control in duplex and triplex DNA-directed chemical reactionsEmail this article to a colleague.
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Mikkel F. Jacobsen, Jens B. Ravnsbaek, Kurt V. Gothelf
(Communication from Org. Biomol. Chem.)
Mikkel F. Jacobsen, Org. Biomol. Chem., 2010, DOI: 10.1039/b919387a
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Source: RSC - Organic Biomolecular Chemistry - November 9, 2009 Category: Molecular Biology Authors: Mikkel F. JacobsenJens B. Ravnsbaek Kurt V. Gothelf Source Type: journals
Flow cytometric measurements of TB-specific T cells comparing with QuantiFERON-TB goldEmail this article to a colleague.
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Interferon-[gamma] (IFN-[gamma]) release assays and the detection of IFN-[gamma] synthesis in the cytoplasm of activated CD4+ T cells by flow cytometry have recently been used for tuberculosis (TB) diagnosis. The aim of this study was to compare the performance of IFN-[gamma] assay between ELISA (QuantiFERON-TB Gold, QFT) and intracellular cytokine flow cytometry (ICCFC), and to investigate the significance of an optimal gating strategy in ICCFC.The CD4+ T cell response to TB antigens was measured using the intracellular cytokine staining technique and four color FC (CD3, CD4, IFN-[gamma], and tumor necrosis factor-[alpha]...
Source: Cytometry Part B: Clinical Cytometry - November 9, 2009 Category: Molecular Biology Authors: Dong Il Won, Jung Ran Park Source Type: journals
Rapid radiation in spiny lobsters (Palinurus spp) as revealed by classic and ABC methods using mtDNA and microsatellite dataEmail this article to a colleague.
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Conclusions:
The Palinurus speciation pattern is a typical example of a series of rapid speciation events occurring within a group, with very short branches separating different species. Our results support the hypothesis that recent climate change-related oceanographic processes have influenced the phylogeny of marine taxa, with most Palinurus species originating during the last two million years. The present study highlights the value of new coalescent-based statistical methods such as ABC for testing different speciation hypotheses using molecular data.
Source: BMC Evolutionary Biology - Latest articles - November 9, 2009 Category: Molecular Biology Authors: Ferran PaleroJoao LopesPere AbelloEnrique MacphersonMarta PascualMark Beaumont Source Type: journals
Absolute structural elucidation of natural products-A focus on quantum-mechanical calculations of solid-state CD spectra.Email this article to a colleague.
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In this review article we examine state-of-the-art techniques for the structural elucidation of organic compounds isolated from natural sources. In particular, we focus on the determination of absolute configuration (AC), perhaps the most challenging but inevitable step in the whole process, especially when newly isolated compounds are screened for biological activity. Among the many methods employed for AC assignment that we review, special attention is paid to electronic circular dichroism (CD) and to the modern tools available for quantum-mechanics CD predictions, including TDDFT. In this context, we stress that con...
Source: Chirality - November 9, 2009 Category: Molecular Biology Authors: Pescitelli G, Kurtán T, Flörke U, Krohn K Tags: Chirality Source Type: journals
Interactions between quercetin and Warfarin for albumin binding: A new eye on food/drug interference.Email this article to a colleague.
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The interaction between quercetin, a popular antioxidant flavonoid, and human serum albumin (HSA) is investigated and characterized by means of induced circular dichroism and saturation transfer difference NMR. These techiques demonstrate the reversible binding of quercetin to the carrier protein, which is responsible for its dissolution in aqueous medium. Competition experiments with two classical probes for HSA binding sites, namely Ibuprofen and Warfarin (a common anticoagulant coumarin), demonstrate that quercetin has a primary binding site located in the subdomain IIA, where coumarins are hosted. The affinity for ...
Source: Chirality - November 9, 2009 Category: Molecular Biology Authors: Di Bari L, Ripoli S, Pradhan S, Salvadori P Tags: Chirality Source Type: journals
Mutant presenilin-1 deregulated peripheral immunity exacerbates alzheimer-like pathology.Email this article to a colleague.
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Abstract Mutations in the presenilin-1 (PS1) gene are independent causes of familial Alzheimer's disease (AD). AD patients have dysregulated immunity, and PS1 mutant mice exhibit abnormal systemic immune responses. To test whether immune function abnormality caused by a mutant human PS1 gene (mhPS1) could modify AD-like pathology, we reconstituted immune systems of AD model mice carrying a mutant human amyloid precursor protein gene (mhAPP; Tg2576 mice) or both mhAPP and mhPS1 genes (PSAPP mice) with allogeneic bone marrow cells. Here, we report a marked reduction in amyloid-beta (Abeta) levels, beta-amyloid plaques, a...
Source: J Cell Mol Med - November 9, 2009 Category: Molecular Biology Authors: Zhu Y, Obregon D, Hou H, Giunta B, Erhart J, Fernandez F, Mori T, Nikolic W, Zhao Y, Morgan D, Town T, Tan J Tags: J Cell Mol Med Source Type: journals
Hypoxia-inducible factors in stem cells and cancer.Email this article to a colleague.
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Abstract Cellular properties are influenced by complex factors inherent to their microenvironments. While oxygen deprivation (hypoxia) occurs in tumors due to rapid cell proliferation and aberrant blood vessel formation, embryonic cells develop in a naturally occurring hypoxic environment. Cells respond to hypoxia by stabilizing hypoxia-inducible factors (HIFs), which are traditionally viewed to function by altering cellular metabolism and blood vessel architecture. Recently, HIFs have been shown to modulate specific stem cell effectors, such as Notch, Wnt, and Oct4, that control stem cell proliferation, differentiatio...
Source: J Cell Mol Med - November 9, 2009 Category: Molecular Biology Authors: Mazumdar J, Dondeti V, Simon MC Tags: J Cell Mol Med Source Type: journals
Identification of microRNA profiles in docetaxel-resistant human non-small cell lung carcinoma cells (SPC-A1).Email this article to a colleague.
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Abstract Docetaxel has been used as first-line chemotherapy in advanced NSCLC, but further extensive and effective application is prevented by drug resistance. MicroRNAs (miRNAs) have recently been identified as important posttranscriptional regulators, which are involved in various biological processes. The aim of this study was to identify microRNA expression profiles involved in the development of docetaxel resistance in non-small cell lung carcinoma (NSCLC). Here, microarray chip technology was employed to identify miRNA expression profiles in docetaxel-resistant human NSCLC cell line (SPC-A1/docetaxel). Then, the ...
Source: J Cell Mol Med - November 9, 2009 Category: Molecular Biology Authors: Rui W, Bing F, Hai-Zhu S, Wei D, Long-Bang C Tags: J Cell Mol Med Source Type: journals
Functional alterations of myeloid cell subsets in hyperlipidemia: relevance for atherosclerosis.Email this article to a colleague.
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Abstract Atherosclerosis is a chronic inflammatory disease wherein the infiltration of myeloid cells of the vessel wall is a hallmark event. Lymphocytes, platelets and endothelial cells stand out as prominent suspects being involved in atherosclerosis. However, recent advances suggest a crucial role for myeloid leukocytes, specifically monocyte subsets, neutrophils, dendritic cells, and endothelial progenitor cells. These cell types are not just rapidly recruited or already reside in the vascular wall, but also initiate and perpetuate core mechanisms in plaque formation and destabilization. Hyperlipidemia is an indepen...
Source: J Cell Mol Med - November 9, 2009 Category: Molecular Biology Authors: Soehnlein O, Drechsler M, Hristov M, Weber C Tags: J Cell Mol Med Source Type: journals
RNA-targeting approaches for neuromuscular diseases.Email this article to a colleague.
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Although most molecular therapy strategies for genetic diseases are based on gene replacement, interesting alternative approaches target RNA. These strategies rely on the modification of the mutated gene's expression in vivo by modulating pre-mRNA splicing, mRNA stability or mRNA translation. Here, we review recent progress using these RNA-based approaches in the field of muscle and muscle-related genetic diseases. Different molecular tools, including modified antisense oligonucleotides, pre-mRNA trans-splicing molecules, ribozymes or chemical compounds have been used successfully on patient cells or animal models of d...
Source: Trends in Molecular Medicine - November 9, 2009 Category: Molecular Biology Authors: Le Roy F, Charton K, Lorson CL, Richard I Tags: Trends Mol Med Source Type: journals
Cooperative effect of ribosomal protein s19 and Pim-1 kinase on murine c-Myc expression and myeloid/erythroid cellularityEmail this article to a colleague.
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Abstract Diamond-Blackfan anemia is a bone marrow failure syndrome associated with heterozygous mutations in the ribosomal protein
S19 (RPS19) gene in a subgroup of patients. One of the interacting partners with RPS19 is the oncoprotein PIM-1 kinase. We intercrossed
Rps19
+/−
and Pim-1
−/−
mice strains to study the effect from the disruption of both genes. The double mutant (Rps19
+/−
Pim-1
−/−
) mice display normal growth with increased peripheral white and red blood cell counts when compared to the w.t. mice (Rps19
+/+
Pim-1
+/+
). Molecular analysis of bone marrow cel...
Source: Journal of Molecular Medicine - November 8, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
N-acetyltransferase 1 and 2 gene sequence variants and risk of head and neck cancer.Email this article to a colleague.
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In this study the role of genetically determined individual NAT1 and NAT2 genotypes, haplotypes and haplotype combinations in the predisposition to head and neck cancer was investigated. Polymorphic regions of the NAT1 and NAT2 genes were analyzed in patients with head and neck cancer and healthy individuals by polymerase chain reaction-restriction fragment length polymorphism. Distribution of the genotypes, allele frequencies, diplotypes and haplotypes and correlation with clinical characteristics were evaluated. No association was observed between the NAT1*3, NAT1*10, NAT1*11, NAT2*5 and NAT2*6 genotypes and risk of head...
Source: Molecular Biology Reports - November 8, 2009 Category: Molecular Biology Authors: Demokan S, Suoglu Y, Gözeler M, Demir D, Dalay N Tags: Mol Biol Rep Source Type: journals
Neuroendocrine circuitry and endometriosis: progesterone derivative dampens corticotropin-releasing hormone-induced inflammation by peritoneal cells in vitroEmail this article to a colleague.
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Abstract Clinical symptoms of endometriosis, such as pain and infertility, can be described as persistent stressors. Such continuous
exposure to stress may severely affect the equilibrium and bidirectional communication of the endocrine and immune system,
hereby further aggravating the progression of endometriosis. In the present study, we aimed to tease apart mediators that
are involved in the stress response as well as in the progression of endometriosis. Women undergoing diagnostic laparoscopy
due to infertility were recruited (n = 69). Within this cohort, early stage of endometriosis were diagnosed i...
Source: Journal of Molecular Medicine - November 7, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
High-Affinity αvβ3 Integrin Targeted Optical Probe as a New Imaging Biomarker for Early Atherosclerosis: Initial Studies in Watanabe RabbitsEmail this article to a colleague.
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Conclusions This high-affinity ITOP identifies the site and extent of αvβ3 expression and correlates with adventitial thickness. Recent
evidence associates αvβ3 expression with the inflammatory process in early vulnerable plaque, making this compound a promising
potential biomarker for early atherosclerotic disease.
Content Type Journal ArticleCategory Brief ArticleDOI 10.1007/s11307-009-0242-zAuthors
Julie Heroux, National Institute of Diabetes Digestive and Kidney Diseases Laboratory of Integrative Cardiovascular Imaging Science Bethesda MD 20892 USAAhmed M. Gharib, National Institute of Diabete...
Source: Molecular Imaging and Biology - November 7, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
Per-channel basis normalization methods for flow cytometry dataEmail this article to a colleague.
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Between-sample variation in high-throughput flow cytometry data poses a significant challenge for analysis of large-scale data sets, such as those derived from multicenter clinical trials. It is often hard to match biologically relevant cell populations across samples because of technical variation in sample acquisition and instrumentation differences. Thus, normalization of data is a critical step before analysis, particularly in large-scale data sets from clinical trials, where group-specific differences may be subtle and patient-to-patient variation common. We have developed two normalization methods that remove technic...
Source: Cytometry Part A - November 7, 2009 Category: Molecular Biology Authors: Florian Hahne, Alireza Hadj Khodabakhshi, Ali Bashashati, Chao-Jen Wong, Randy D. Gascoyne, Andrew P. Weng, Vicky Seyfert-Margolis, Katarzyna Bourcier, Adam Asare, Thomas Lumley, Robert Gentleman, Ryan R. Brinkman Source Type: journals
Deadly liaisons: fatal attraction between CCN matricellular proteins and the tumor necrosis factor family of cytokines.Email this article to a colleague.
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Recent studies have revealed an unexpected synergism between two seemingly unrelated protein families: CCN matricellular proteins and the tumor necrosis factor (TNF) family of cytokines. CCN proteins are dynamically expressed at sites of injury repair and inflammation, where TNF cytokines are also expressed. Although TNFalpha is an apoptotic inducer in some cancer cells, it activates NFkappaB to promote survival and proliferation in normal cells, and its cytotoxicity requires inhibition of de novo protein synthesis or NFkappaB signaling. The presence of CCN1, CCN2, or CCN3 overrides this requirement and unmasks the apo...
Source: Journal of Cell Communication and Signaling - November 7, 2009 Category: Molecular Biology Authors: Chen CC, Lau LF Tags: J Cell Commun Signal Source Type: journals
Erratum to: Origins of Life and Evolution of Biospheres.Email this article to a colleague.
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PMID: 19898951 [PubMed - as supplied by publisher]
Source: Origins of Life and Evolution of the Biosphere - November 7, 2009 Category: Molecular Biology Authors: Macdermott AJ Tags: Orig Life Evol Biosph Source Type: journals
Plasma Cytokine Profiles in Preprotachykinin-A Knockout Mice Subjected to Polymicrobial Sepsis.Email this article to a colleague.
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During the course of polymicrobial sepsis, a range of pro- and anti-inflammatory cytokines are produced by the host immune system. Successful recovery from sepsis involves striking a balance between these counteracting cytokines. We herein investigated the circulating cytokine profiles in preprotachykinin-A knockout (PPTA(-/-)) mice, which have been found to be significantly protected against microbial sepsis, by employing multiplexed bead-based suspension arrays for the measurement of 18 plasma cytokines. Four sets of PPTA(-/-) and wild-type mice, each with 6 mice, were subjected to cecal ligation and puncture-induced...
Source: Molecular Medicine - November 7, 2009 Category: Molecular Biology Authors: Hegde A, Uttamchandani M, Moochhala SM, Bhatia M Tags: Mol Med Source Type: journals
Effects of carbon monoxide releasing molecule-liberated CO on severe acute pancreatitis in rats.Email this article to a colleague.
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In this study, we investigated the role of CO liberated from carbon monoxide releasing molecule-2 (CORM-2) in rats with SAP. SAP was induced by retrograde infusion of 5% sodium taurocholate into the pancreatobiliary duct. Forty Wistar rats were randomly divided into four groups. Sham group was given normal saline after the sham operation. SAP group was treated with normal saline after the induction of SAP. CORM-2 group was injected with CORM-2 (8mg/kg, i.v.) after the onset of SAP. iCORM-2 group was given iCORM-2 (an inactive compound used as negative control) after SAP induction. All animals were sacrificed at 12h after t...
Source: Cytokine - November 7, 2009 Category: Molecular Biology Authors: Chen P, Sun B, Chen H, Wang G, Pan S, Kong R, Bai X, Wang S Tags: Cytokine Source Type: journals
Plasma interleukin-1beta concentration is associated with stroke in sickle cell disease.Email this article to a colleague.
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The pathogenesis of sickle cell disease (HbSS), which has numerous complications including stroke, involves inflammation resulting in alteration of plasma inflammatory protein concentration. We investigated HbSS children with abnormal cerebral blood flow detected by trans-cranial Doppler ultrasound (TCD) who participated in the multi-center stroke prevention (STOP) study, to determine if plasma inflammatory protein concentration is associated with the outcome of stroke. Thirty-nine plasma samples from HbSS participants with elevated TCD who had no stroke, HbSS-NS (n=13) or had stroke, HbSS-S (n=13), HbSS steady-state c...
Source: Cytokine - November 7, 2009 Category: Molecular Biology Authors: Asare K, Gee BE, Stiles JK, Wilson NO, Driss A, Quarshie A, Adams RJ, Kutlar A, Hibbert JM Tags: Cytokine Source Type: journals
The pneumococcus: why a commensal misbehavesEmail this article to a colleague.
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Abstract Several characteristics of Streptococcus pneumoniae (pneumococcus) combine to make it a particularly problematic pathogen. Firstly, the pneumococcus has the capacity to cause
disease through the expression of virulence factors such as its polysaccharide capsule and pore-forming toxin. In addition,
the pneumococcus is highly adaptable as demonstrated by its ability to acquire and disseminate resistance to multiple antibiotics.
Although the pneumococcus is a major cause of disease, the organism is most commonly an “asymptomatic” colonizer of its human
host (the carrier state), with transmission oc...
Source: Journal of Molecular Medicine - November 6, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Host-guest complexations of local anaesthetics by cucurbit[7]uril in aqueous solutionEmail this article to a colleague.
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Ian W. Wyman, Donal H. Macartney
(Paper from Org. Biomol. Chem.)
Ian W. Wyman, Org. Biomol. Chem., 2010, DOI: 10.1039/b915694a
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Source: RSC - Organic Biomolecular Chemistry - November 6, 2009 Category: Molecular Biology Authors: Ian W. WymanDonal H. Macartney Source Type: journals
Asymmetric ruthenium-catalyzed 1,4-additions of aryl thiols to enonesEmail this article to a colleague.
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Andrei Badoiu, Gerald Bernardinelli, Celine Besnard, E. Peter Kundig
(Paper from Org. Biomol. Chem.)
Andrei Badoiu, Org. Biomol. Chem., 2010, DOI: 10.1039/b918877k
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Source: RSC - Organic Biomolecular Chemistry - November 6, 2009 Category: Molecular Biology Authors: Andrei BadoiuGerald Bernardinelli Celine Besnard E. Peter Kundig Source Type: journals
On the Emergence of Biochemical Homochirality: An Elusive Beginning.Email this article to a colleague.
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PMID: 19890734 [PubMed - as supplied by publisher]
Source: Origins of Life and Evolution of the Biosphere - November 6, 2009 Category: Molecular Biology Authors: Pizzarello S, Lahav M Tags: Orig Life Evol Biosph Source Type: journals
BH3-only proteins: The death-puppeteer's wiresEmail this article to a colleague.
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Most cell death in vertebrates proceeds through the intrinsic pathway of apoptosis and results from unregulated increase of mitochondrial membrane permeability. Bcl2-associated X protein (Bax) and Bcl2-antagonist/killer protein (Bak), the effector proapoptotic members of the Bcl-2 family, are, in their active state, the principal accomplices for this permeabilization process. How exactly Bax and Bak are activated has been a matter of major investigation in the last decade, and suitable tools offered by quantitative cytometric methodologies have significantly contributed to the understanding of the function of Bcl-2 family ...
Source: Cytometry Part A - November 6, 2009 Category: Molecular Biology Authors: Fabio Ghiotto, Franco Fais, Silvia Bruno Source Type: journals
A quantitative measure for alterations in the actin cytoskeleton investigated with automated high-throughput microscopyEmail this article to a colleague.
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The actin cytoskeleton modulates a large variety of physiological and disease-related processes in the cell. For example, actin has been shown to be a crucial host factor for successful infection by HIV-1, but the underlying mechanistic details are still unknown. Automated approaches open up the perspective to clarify such an issue by processing many samples in a high-throughput manner. To analyze the alterations in the actin cytoskeleton within an automated setting, large-scale image acquisition and analysis were established for JC-53 cells stained for actin. As a quantitative measure in such an automated approach, we sug...
Source: Cytometry Part A - November 6, 2009 Category: Molecular Biology Authors: Julian Weichsel, Nikolas Herold, Maik J. Lehmann, Hans-Georg Kräusslich, Ulrich S. Schwarz Source Type: journals
Flow immunocytochemistry of marker expression in cells from body cavity fluidsEmail this article to a colleague.
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Diagnostic cytology based on the examination of cells from body cavity fluids misses [sim]50% of patients with a proven malignancy. In an earlier study, we used immunohistochemical detection of epithelial membrane antigen expression with flow cytometric detection of DNA aneuploidy to reduce the number of false negatives. In the present study, we have combined DNA flow cytometry with flow cytometric detection of marker expression to analyze cells from body cavity fluids. Seventy-nine specimens of ascites and pleural fluids were analyzed by diagnostic cytology, DNA flow cytometry, and for the expression of the following mark...
Source: Cytometry Part A - November 6, 2009 Category: Molecular Biology Authors: Awtar Krishan, Parvin Ganjei-Azar, Ronald Hamelik, Deepti Sharma, Isildinha Reis, Mehrdad Nadji Source Type: journals
Computational and Systems BiologyEmail this article to a colleague.
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(Editorial from Mol. BioSyst.)
Mol. BioSyst., 2009, DOI: 10.1039/b921381n
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Source: RSC - Mol. BioSyst. latest articles - November 6, 2009 Category: Molecular Biology Authors: RSC Source Type: journals
NYGGF4 homologous gene expression in 3T3-L1 adipocytes: regulation by FFA and adipokines.Email this article to a colleague.
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NYGGF4 is a novel gene that is abundantly expressed in the adipose tissue of obese subjects and is involved in insulin resistance. In the present study, the mRNA expression of NYGGF4 homologous genes was examined in the 3T3-L1 cell line. The NYGGF4 mRNAs were expressed at low levels in the 3T3-L1 preadipocytes. During the conversion of 3T3-L1 preadipocytes to adipocytes, the expression of NYGGF4 mRNA was upregulated. On the 8th day after induction of differentiation, the NYGGF4 mRNA levels peaked and remained high. Free fatty acids (FFA) and tumor necrosis factor-alpha (TNFalpha) could upregulate NYGGF4 mRNA expression...
Source: Molecular Biology Reports - November 6, 2009 Category: Molecular Biology Authors: Zhao YP, Zhang CM, Zhu C, Chen XH, Wang JL, Ji CB, Chi X, Hong Q, Peng YZ, Guo XR Tags: Mol Biol Rep Source Type: journals
Chiral configuration of the hydration layers of D- and L-alanine in water implied from dilution calorimetry.Email this article to a colleague.
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In this study, we determined by dilution microcalorimetry the fraction of the hydration energy invested in the chiral distortion of the hydration layer surrounding D- and I-alanine in water. The results indicate that in dilute solutions, a multilayered chiral hydration surrounds each of these solutes and amounts to over 100 water molecules. In concentrated solutions, the immediate chiral hydration layer decreases to approximately 30 water molecules. The energy invested in the induction of the chiral twist in the hydration layer is predominantly attributed to TDeltaS, the energy associated with "configurational entropy," wh...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Shvalb A, Mastai Y, Shinitzky M Tags: Chirality Source Type: journals
An efficient dipeptide-catalyzed direct asymmetric aldol reaction of equimolar reactants in solid media.Email this article to a colleague.
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The direct asymmetric aldol reactions of equivalent molar amounts of aldehydes and ketones were carried out at -20 degrees C over alkaline Al(2)O(3) with 20 mol % of Pro-Trp as catalyst and 20 mol % of N-methylmorpholine or 1,4-diazabicyclo[2.2.2]octane as additive. After simple and environmentally friendly work-up, moderate to high isolated yields (up to 95%), good diastereoselectivities (>99:1), and enantioselectivities (up to 98% ee) have been achieved for the reactions of different kinds of ketones with various aldehydes. The catalytic system could be reused without decrease of activity by addition of 10 mol % c...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Lei M, Xia S, Wang J, Ge Z, Cheng T, Li R Tags: Chirality Source Type: journals
Highly stereoselective hydrogenations-As key-steps in the total synthesis of statins.Email this article to a colleague.
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Statins are inhibitors of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase (HMG-CoA reductase) and became the standard of care for treatment of hypercholesterolemia because of their efficacy, safety, and long-term benefits. They are administered as diastereo- and enantiomerically pure compounds. We summarize here two new approaches for the total synthesis of the most important representatives, atorvastatin, and rosuvastatin, based on highly stereoselective hydrogenations as key-steps. Chirality 2010. (c) 2009 Wiley-Liss, Inc.
PMID: 19899159 [PubMed - as supplied by publisher]
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Andrushko N, Andrushko V, Tararov V, Korostylev A, König G, Börner A Tags: Chirality Source Type: journals
Enantioselective interaction with acetylcholinesterase of an organophosphate insecticide fenamiphos.Email this article to a colleague.
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In this study, we used fenamiphos (FAP), an organophosphorus insecticide, to study enantioselectivity in toxicity to arthropods and the inhibition potential towards acetylcholinesterase (AChE) in the rat pheochromocytoma 12 (PC 12) cell line. Furthermore, we carried out molecular docking to help explain the mechanisms of enantioselective toxicity of FAP. The two enantiomers of FAP were successfully separated and identified as R-(+)-FAP and S-(-)-FAP. Toxicological assays revealed that R-(+)-FAP was 2.4-fold more toxic than S-(-)-FAP to Daphnia magna and approximately threefold more to PC12 cells. Based on molecular docking...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Wang C, Zhang N, Li L, Zhang Q, Zhao M, Liu W Tags: Chirality Source Type: journals
Molecular and supramolecular chirality in gemini-tartrate amphiphiles studied by electronic and vibrational circular dichroisms.Email this article to a colleague.
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This contribution presents an application of electronic circular dichroism (ECD) and vibrational circular dichroism (VCD) to study the molecular and supramolecular chirality in assemblies of gemini-tartrate amphiphiles. Nonchiral dicationic n-2-n amphiphiles (n = 14-20) can self-organize into right- or left-handed structures upon interacting with chiral tartrate counterions. Micellar solutions can also be obtained for shorter alkyl chains (n = 12). First, the conformation of tartrate counterions has been investigated in various environments (micellar solutions and chiral ribbons). ECD and VCD spectra recorded in micell...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Brizard A, Berthier D, Aimé C, Buffeteau T, Cavagnat D, Ducasse L, Huc I, Oda R Tags: Chirality Source Type: journals
Preparation and enantioseparation of a mixed selector chiral stationary phase derived from benzoylated tartaric acid and 1,2-diphenylethylenediamine.Email this article to a colleague.
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L-Dibenzoyl tartaric acid was mono-esterified with benzyl alcohol, and then chlorinated with SOCl(2) to give (2S,3S)-1-(benzyloxy)-4-chloro-1,4-dioxobutane-2,3-diyl dibenzoate (Selector 1). (1R,2R)-1,2-Diphenylethylenediamine was mono-functionalized with phenyl isocyanate and phenylene diisocyanate in sequence to give (1R,2R)-1,2-diphenyl-2-(3-phenylureido)ethyl 4- isocyanatophenylurea (Selector 2). Two brush-type chiral stationary phases (CSPs) of single selector were prepared by separately immobilizing selectors 1 and 2 on aminated silica gel. Selectors 1 and 2 were simultaneously immobilized on aminated silica gel t...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Wei WJ, Deng HW, Chen W, Bai ZW, Li SR Tags: Chirality Source Type: journals
Diastereoisomeric assignment in a pacifenol derivative using vibrational circular dichroism.Email this article to a colleague.
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The configuration of a chiral center in semisynthetic (-)-(2R,5R,5aR,8zeta,9aS)- 2,8-dibromo-2,5,9,9a-tetrahydro-5-hydroxy-5,8,10,10-tetramethyl-6H-2,5a-methano-1-benzoxepin-7(8H)-one (3 or 4), prepared in two steps from (-)-(2R,5R,5aR,7S,8S,9aS)-2, 7-dibromo-8-chloro-2,5,7,8,9,9a-hexahydro-5,8,10,10-tetramethyl-6H-2,5a-methano-1-benzoxepin-5-ol, known as pacifenol 1, has been determined using vibrational circular dichroism (VCD) measurements. The vibrational spectra (IR and VCD) of diastereoisomers 3 and 4 were calculated using density functional theory (DFT) at the B3LYP/DGDZVP level of theory for the two conformers ...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Muñoz MA, Chamy C, Carrasco A, Rovirosa J, Martín AS, Joseph-Nathan P Tags: Chirality Source Type: journals
Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen.Email this article to a colleague.
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The solvent versatility of Chiralpak IB, a 3,5-dimethylphenylcarbamate derivative of cellulose-based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase-catalysis and in mean time as diluent to dissolve the "difficult to dissolve" enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization. Chi...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Ghanem A Tags: Chirality Source Type: journals
Determination of the absolute configurations at stereogenic centers in the presence of axial chirality.Email this article to a colleague.
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Cephalochromin, a homodimeric naphthpyranone natural product, contains both axial chirality due to the hindered rotation along the biaryl axis and central chirality due to the C-2, C-2' stereogenic centers of the fused pyranone ring. For determining the absolute configurations (ACs) of central chirality elements, different chiroptical spectroscopic methods, namely vibrational circular dichroism (VCD), electronic circular dichroism (ECD), and optical rotation (OR), have been used. From these experimental data, in conjunction with corresponding quantum chemical predictions at B3LYP/6-311G* level, it is found that the ECD...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Polavarapu PL, Jeirath N, Kurtán T, Pescitelli G, Krohn K Tags: Chirality Source Type: journals
Stereochemical study of a novel tautomeric furanone, homofuraneol.Email this article to a colleague.
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A mixture of tautomers with unique keto-enol structures, 5-ethyl-4-hydroxy-2-methylfuran-3(2H)-one or 2-ethyl-4-hydroxy-5-methylfuran-3(2H)-one (EHMF, homofuraneol, 1a or 1b), comprises four structural isomers including their enantiomers. The four isomers were successfully separated by chromatographic optical resolution, and their odor evaluation was performed. Determination of the absolute chemistry of 1a and 1b were accomplished for the first time by direct measurement of the VCD spectra of their methyl ether derivatives 4a and 4b compared with the calculated ones as well as chemical relay reaction. The relationship ...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Monde K, Nakahashi A, Miura N, Yoshihiro Y, Sugimoto D, Emura M Tags: Chirality Source Type: journals
Structural determination of molecular stereochemistry using VCD spectroscopy and a conformational code: Absolute configuration and solution conformation of a chiral liquid pesticide, (R)-(+)-malathion.Email this article to a colleague.
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The absolute configuration and solution conformation of (R)-(+)-malathion were determined by using vibrational circular dichroism spectroscopy and a fragment-conformational search with a recently published conformational code. The determination of molecular stereochemistry was carried out without a conformational search using molecular mechanics calculations. Density functional theory calculations of the fragments of (R)-malathion, ethyl propionate, (R)-ethyl 2-(methylthio)propanoate, (R)-diethyl 2-(methylthio)succinate, and O,O,S-trimethyl phosphorodithioate were carried out, and the principal conformational features ...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Izumi H, Ogata A, Nafie LA, Dukor RK Tags: Chirality Source Type: journals
TD-DFT modeling of the circular dichroism for a tryptophan zipper peptide with coupled aromatic residues.Email this article to a colleague.
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In this work, time dependent density functional theory (TD-DFT) is used to provide a reliable basis for interpretation of the electronic spectra of coupled tryptophan (Trp) residues, particularly those in a model Trpzip beta-hairpin peptide. Pairs of isolated indoles form chiral coupled chromophores whose computed electronic ultraviolet circular dichroism (CD) is in excellent agreement with observed transition wavelengths and intensities. The calculations were compared to experimental data for pairwise coupling in mutant Trpzip peptides that are recently available. A study of variation of the basis set, geometry optimi...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Roy A, Bouř P, Keiderling TA Tags: Chirality Source Type: journals
Antagonism of α2A-adrenoceptor: a novel approach to inhibit inflammatory responses in sepsisEmail this article to a colleague.
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In conclusion, modulation of the sympathetic nervous system by blocking α2A-AR appears to be a novel treatment for inflammatory conditions such as sepsis.
Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00109-009-0555-zAuthors
Fangming Zhang, The Feinstein Institute for Medical Research Manhasset NY 11030 USARongqian Wu, The Feinstein Institute for Medical Research Manhasset NY 11030 USAXiaoling Qiang, The Feinstein Institute for Medical Research Manhasset NY 11030 USAMian Zhou, The Feinstein Institute for Medical Research Manhasset NY 11030 USAPing Wang, The Feinstein Institute for Medical Research...
Source: Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Ghrelin in gastrointestinal diseases and disorders: a possible role in the pathophysiology and clinical implications (review).Email this article to a colleague.
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Ghrelin is a peptide hormone, which has been isolated from the stomach. It is localized mostly in endocrine cells in the oxyntic mucosa of the stomach. Ghrelin receptors are expressed equally in all parts of the gastrointestinal tract, with a similar level of expression in the mucosal and muscle layers. This peptide hormone has several functions, the most widely known is its growth hormone (GH)-releasing effect. Ghrelin plays an important role in regulating appetite, feeding and energy metabolism. It also plays a role in mediating immune response and inflammatory processes. Ghrelin stimulates gastric motility and empty...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: El-Salhy M Tags: Int J Mol Med Source Type: journals
Diagnosis of parathyroid carcinoma using immunohistochemical staining against hTERT.Email this article to a colleague.
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The differential diagnosis of parathyroid carcinoma from benign adenoma is often difficult when its typical clinicopathological features are absent, even with the aid of various molecular markers. We recently demonstrated that telomerase activation through hTERT expression is a unique characteristic that is limited to parathyroid carcinoma and not seen in benign tumors. In the present study, we investigated hTERT expression in parathyroid tumors using immunohistochemistry in an attempt to determine its clinical utility. There was no evidence of immunoreactivity in the 4 normal parathyroid glands and the 18 typical aden...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Osawa N, Onoda N, Kawajiri H, Tezuka K, Takashima T, Ishikawa T, Miyauchi A, Hirokawa M, Wakasa K, Hirakawa K Tags: Int J Mol Med Source Type: journals
Neuronal and BBB damage induced by sera from patients with secondary progressive multiple sclerosis.Email this article to a colleague.
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An important component of the pathogenic process of multiple sclerosis (MS) is the blood-brain barrier (BBB) damage. We recently set an in vitro model of BBB, based on a three-cell-type co-culture system, in which rat neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological BBB. Herein we report that the serum from patients with secondary progressive multiple sclerosis (SPMS) has a damaging effect on isolated neurons. This finding suggests that neuronal damaging in MS could be a primary event and not only secon...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Proia P, Schiera G, Salemi G, Ragonese P, Savettieri G, Di Liegro I Tags: Int J Mol Med Source Type: journals
Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma.Email this article to a colleague.
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We describe a novel solid-state nuclear magnetic resonance (NMR) method that maximizes the advantages of high-resolution magic-angle-spinning (HRMAS), relative conventional liquid-state NMR approaches, when applied to intact biopsies of skeletal muscle specimens collected from burn trauma patients. This novel method, termed optimized adiabatic TOtal through Bond correlation SpectroscopY (TOBSY) solid-state NMR pulse sequence for two-dimensional (2D) 1H-1H homonuclear scalar-coupling longitudinal isotropic mixing, was demonstrated to provide a 40-60% improvement in signal-to-noise ratio (SNR) relative to its liquid-state an...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Righi V, Andronesi O, Mintzopoulos D, Tzika AA Tags: Int J Mol Med Source Type: journals
Human platelet 12-lipoxygenase: naturally occurring Q261/R261 variants and N544L mutant show altered activity but unaffected substrate binding and membrane association behavior.Email this article to a colleague.
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The single nucleotide polymorphism (SNP) R261Q in the human platelet 12-lipoxygenase has been correlated with several human diseases. To understand better the biological performance we have compared enzymatic properties of the recombinant enzymes: 'wild-type' as Q261 and R261 variants with a single Q261R mutation at the enzyme periphery and N544L mutant with an altered active site. The R261 variant does not follow the same kinetics such as WT-Q261 showing a lag phase, a slower accumulation of product, following a different time-course without reaching plateau characteristic for the Q261 variant. The N544L substitution ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Aleem AM, Wells L, Jankun J, Walther M, Kühn H, Reinartz J, Skrzypczak-Jankun E Tags: Int J Mol Med Source Type: journals
