Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 6.
TD-DFT modeling of the circular dichroism for a tryptophan zipper peptide with coupled aromatic residues.
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In this work, time dependent density functional theory (TD-DFT) is used to provide a reliable basis for interpretation of the electronic spectra of coupled tryptophan (Trp) residues, particularly those in a model Trpzip beta-hairpin peptide. Pairs of isolated indoles form chiral coupled chromophores whose computed electronic ultraviolet circular dichroism (CD) is in excellent agreement with observed transition wavelengths and intensities. The calculations were compared to experimental data for pairwise coupling in mutant Trpzip peptides that are recently available. A study of variation of the basis set, geometry optimi...
Source: Chirality - November 6, 2009 Category: Molecular Biology Authors: Roy A, Bouř P, Keiderling TA Tags: Chirality Source Type: journals
Antagonism of α2A-adrenoceptor: a novel approach to inhibit inflammatory responses in sepsisEmail this article to a colleague.
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In conclusion, modulation of the sympathetic nervous system by blocking α2A-AR appears to be a novel treatment for inflammatory conditions such as sepsis.
Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00109-009-0555-zAuthors
Fangming Zhang, The Feinstein Institute for Medical Research Manhasset NY 11030 USARongqian Wu, The Feinstein Institute for Medical Research Manhasset NY 11030 USAXiaoling Qiang, The Feinstein Institute for Medical Research Manhasset NY 11030 USAMian Zhou, The Feinstein Institute for Medical Research Manhasset NY 11030 USAPing Wang, The Feinstein Institute for Medical Research...
Source: Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals
Ghrelin in gastrointestinal diseases and disorders: a possible role in the pathophysiology and clinical implications (review).Email this article to a colleague.
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Ghrelin is a peptide hormone, which has been isolated from the stomach. It is localized mostly in endocrine cells in the oxyntic mucosa of the stomach. Ghrelin receptors are expressed equally in all parts of the gastrointestinal tract, with a similar level of expression in the mucosal and muscle layers. This peptide hormone has several functions, the most widely known is its growth hormone (GH)-releasing effect. Ghrelin plays an important role in regulating appetite, feeding and energy metabolism. It also plays a role in mediating immune response and inflammatory processes. Ghrelin stimulates gastric motility and empty...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: El-Salhy M Tags: Int J Mol Med Source Type: journals
Diagnosis of parathyroid carcinoma using immunohistochemical staining against hTERT.Email this article to a colleague.
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The differential diagnosis of parathyroid carcinoma from benign adenoma is often difficult when its typical clinicopathological features are absent, even with the aid of various molecular markers. We recently demonstrated that telomerase activation through hTERT expression is a unique characteristic that is limited to parathyroid carcinoma and not seen in benign tumors. In the present study, we investigated hTERT expression in parathyroid tumors using immunohistochemistry in an attempt to determine its clinical utility. There was no evidence of immunoreactivity in the 4 normal parathyroid glands and the 18 typical aden...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Osawa N, Onoda N, Kawajiri H, Tezuka K, Takashima T, Ishikawa T, Miyauchi A, Hirokawa M, Wakasa K, Hirakawa K Tags: Int J Mol Med Source Type: journals
Neuronal and BBB damage induced by sera from patients with secondary progressive multiple sclerosis.Email this article to a colleague.
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An important component of the pathogenic process of multiple sclerosis (MS) is the blood-brain barrier (BBB) damage. We recently set an in vitro model of BBB, based on a three-cell-type co-culture system, in which rat neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological BBB. Herein we report that the serum from patients with secondary progressive multiple sclerosis (SPMS) has a damaging effect on isolated neurons. This finding suggests that neuronal damaging in MS could be a primary event and not only secon...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Proia P, Schiera G, Salemi G, Ragonese P, Savettieri G, Di Liegro I Tags: Int J Mol Med Source Type: journals
Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma.Email this article to a colleague.
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We describe a novel solid-state nuclear magnetic resonance (NMR) method that maximizes the advantages of high-resolution magic-angle-spinning (HRMAS), relative conventional liquid-state NMR approaches, when applied to intact biopsies of skeletal muscle specimens collected from burn trauma patients. This novel method, termed optimized adiabatic TOtal through Bond correlation SpectroscopY (TOBSY) solid-state NMR pulse sequence for two-dimensional (2D) 1H-1H homonuclear scalar-coupling longitudinal isotropic mixing, was demonstrated to provide a 40-60% improvement in signal-to-noise ratio (SNR) relative to its liquid-state an...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Righi V, Andronesi O, Mintzopoulos D, Tzika AA Tags: Int J Mol Med Source Type: journals
Human platelet 12-lipoxygenase: naturally occurring Q261/R261 variants and N544L mutant show altered activity but unaffected substrate binding and membrane association behavior.Email this article to a colleague.
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The single nucleotide polymorphism (SNP) R261Q in the human platelet 12-lipoxygenase has been correlated with several human diseases. To understand better the biological performance we have compared enzymatic properties of the recombinant enzymes: 'wild-type' as Q261 and R261 variants with a single Q261R mutation at the enzyme periphery and N544L mutant with an altered active site. The R261 variant does not follow the same kinetics such as WT-Q261 showing a lag phase, a slower accumulation of product, following a different time-course without reaching plateau characteristic for the Q261 variant. The N544L substitution ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Aleem AM, Wells L, Jankun J, Walther M, Kühn H, Reinartz J, Skrzypczak-Jankun E Tags: Int J Mol Med Source Type: journals
Evaluation of annexin II as a potential serum marker for hepatocellular carcinoma using a developed sandwich ELISA method.Email this article to a colleague.
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Annexin II (Annexin A2, ANXA2) is a 36 kDa calcium-dependent phospholipid-binding protein that is located on the surface of most eukaryotic cells. ANXA2 is involved in several biological processes, including anti-inflammatory effects, Ca27+-dependent exocytosis, immune responses, Ca2+ transport and phospholipase A2 regulation. In our previous study, ANXA2 was identified as an up-regulated gene in hepatocellular carcinoma (HCC) tissue by cDNA microarray. In the present study, we have evaluated ANXA2 as a tumor-associated marker of HCC. We determined the ANXA2 levels in human liver tissues with HCC using real-time RT-PCR...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Ji NY, Park MY, Kang YH, Lee CI, Kim DG, Yeom YI, Jang YJ, Myung PK, Kim JW, Lee HG, Kim JW, Lee K, Song EY Tags: Int J Mol Med Source Type: journals
Experimental study of the anti-cancer mechanism of tanshinone IIA against human breast cancer.Email this article to a colleague.
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This study was performed to determine the anti-cancer activity of tanshinone IIA on human breast cancer cells in vitro and in vivo and to elucidate the underlying mechanism of this activity. Human breast cancer cell lines (estrogen receptor-positive and -negative) were treated with tanshinone IIA and tamoxifen. The inhibitory effects of tanshinone IIA and tamoxifen on breast cancer cell proliferation were examined using MTT assays, BrdU incorporation, immunohistochemistry and flow cytometry. Upon treatment with tanshinone IIA, breast cancer cell proliferation was significantly inhibited in a dose- and time-dependent manner...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Lu Q, Zhang P, Zhang X, Chen J Tags: Int J Mol Med Source Type: journals
Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells.Email this article to a colleague.
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Neuropeptides B (NPB) and W (NPW) have been identified as endogenous ligands of two G-protein-coupled receptors, neuropeptides B/W receptor 1 (NPBWR1, formerly known as GPR7) and neuropeptides B/W receptor 2 (NPBWR2, formerly known as GPR8). In rodents where NPBWR2 is absent, its counterpart is named the similar to neuropeptides B/W receptor 2 (similar to NPBWR2, formerly GPR8-like). Both NPB and NPW play a role in the control of feeding, neuroendocrine axis functions, memory and learning processes as well as in pain regulation. The present study aimed to investigate the expression of NPB, NPW, NPBWR1 and the similar t...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Ziolkowska A, Rucinski M, Tyczewska M, Malendowicz LK Tags: Int J Mol Med Source Type: journals
REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells.Email this article to a colleague.
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The reduced expression in immortalized cells (REIC)/Dickkopf (Dkk)-3, a member of the Dkk gene family, is a tumor suppressor in a broad range of cancers. REIC/Dkk-3 transfected stable clones of mouse prostate cancer RM9 cells (RM9-REIC) and the empty vector-transfected control clone cells (RM9-EV) were established. Clones were used to evaluate the anti-cancer effects and a proteomics analysis of REIC/Dkk-3 continuous expression was performed. The RM9-REIC cells show a feeble appearance and the cell membrane shows irregular buds known as blebs. In vitro cell proliferation was significantly suppressed in RM9-REIC clones ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Chen J, Watanabe M, Huang P, Sakaguchi M, Ochiai K, Nasu Y, Ouchida M, Huh NH, Shimizu K, Kashiwakura Y, Kaku H, Kumon H Tags: Int J Mol Med Source Type: journals
Dysfunction of Ca2+/CaM kinase IIalpha cascades in the amygdala in post-traumatic stress disorder.Email this article to a colleague.
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In this study, we investigated changes in Ca2+-CaM-CaMKIIalpha in the basolateral amygdala of rats after SPS which may reveal part of the pathogenesis of PTSD. The intracellular free calcium level in the basolateral amygdala was examined by fluorescence spectrophotometry. CaM and CaMKIIalpha expression in basolateral amygdala was examined using immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction. The intracellular free calcium level in the basolateral amygdala was increased when compared to that in the control group 1 day after SPS exposure (P<0.05). CaM expression significantly in...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Xiao B, Han F, Shi YX Tags: Int J Mol Med Source Type: journals
Lambda-carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells.Email this article to a colleague.
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Previous studies have shown that highly sulfated lambda-carrageenan oligosaccharides (lambda-CO) possess an anti-angiogenetic effect, while high concentrations of lambda-CO present a cytotoxic effect towards human umbilical vein endothelial cells (HUVECs). The aim of this study was to explore the underlying mechanism of lambda-CO on inhibiting cell proliferation. lambda-CO elicited reactive oxygen species (ROS) production with concentrations at 0.8 and 1 mg/ml, and this event was accompanied by the increase of early apoptotic cells, nuclear morphology changes and cell cycle arrest at the S and G2/M phases. However, pre...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Chen HM, Yan XJ, Mai TY, Wang F, Xu WF Tags: Int J Mol Med Source Type: journals
Neurotrophic receptor tyrosine kinase B induces c-fos-associated cell survival.Email this article to a colleague.
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In this study, we established 293T cells stably expressing human TrkB to elucidate its intracellular functions. Using this cell system, we examined the biological roles of TrkB and its downstream target molecules. The TrkB expressing cells showed an increased survival rate through increased c-fos mRNA expression by BDNF, which were completely suppressed by TrkB inhibitor. Moreover, the combination of inhibitors of mitogen-activated protein kinase (MEK) and phosphatidylinositol 3-kinase (PI3K) partially reduced both the cell survival rate and c-fos mRNA expression, whereas monotreatment of these reagents could not affect ce...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Hayashi A, Nakatani K, Nishioka J, Sakamoto Y, Jinda S, Wada H, Nobori T Tags: Int J Mol Med Source Type: journals
Coronary artery disease and depression: possible role of brain-derived neurotrophic factor and serotonin transporter gene polymorphisms.Email this article to a colleague.
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Cardiovascular disease (CVD) and depression are two of the most common human health problems. Patients with depression have an increased risk of developing cardiovascular disease and mortality after experiencing a cardiac event. Both diseases are complex disorders that are influenced by genetic and environmental factors. Brain-derived neuro-trophic factor (BDNF) plays a critical role in regulating both vascular development and response to injury, and promotes survival, differentiation, and maintenance of neurons in the peripheral and nervous system. Evidence suggests that BDNF can enhance serotoninergic transmission. S...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Bozzini S, Gambelli P, Boiocchi C, Schirinzi S, Falcone R, Buzzi P, Storti C, Falcone C Tags: Int J Mol Med Source Type: journals
Glycoprotein extraction from Laminaria japonica promotes IEC-6 cell proliferation.Email this article to a colleague.
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In this study, we isolated a novel glycoprotein from L. japonica that stimulates the growth of the IEC-6 normal murine intestinal epithelial cells. We also identified the mechanism by which this glycoprotein, referred to as LJGP, stimulates cell growth. After 24 h of exposure to LJGP, cell proliferation increased in a dose-dependent manner. To further explore the mechanism associated with LJGP-induced cell proliferation, we treated cells for various times with LJGP. We focused on the epidermal growth factor receptor (EGFR) signaling pathway, which is involved in the regulation of cellular proliferation and differentiation,...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Go H, Hwang HJ, Nam TJ Tags: Int J Mol Med Source Type: journals
Changes in the expression of cholesterol metabolism-associated genes in HCV-infected liver: a novel target for therapy?Email this article to a colleague.
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In this study, we evaluated the expression of lipid metabolism-associated genes in patients with HCV infection by real-time PCR. Sterol regulatory element-binding protein (SREBP)-2 expression was unchanged and low density lipoprotein receptor expression was markedly reduced by 90% in HCV-infected liver. The expression of apolipoprotein B100, microsomal triglyceride transfer protein and ATP-binding cassette G5 was significantly increased. Up-regulation of cholesterol synthesis-associated genes, including HMG-CoA reductase, HMG-CoA synthase, farnesyl-diphosphate synthase and squalene synthase, confirmed enhanced de novo chol...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Nakamuta M, Yada R, Fujino T, Yada M, Higuchi N, Tanaka M, Miyazaki M, Kohjima M, Kato M, Yoshimoto T, Harada N, Taketomi A, Maehara Y, Koga M, Nishinakagawa T, Nakashima M, Kotoh K, Enjoji M Tags: Int J Mol Med Source Type: journals
Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease.Email this article to a colleague.
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Crohn's disease is associated with increased permeability of the intestine even in quiescent patients. Increased intestinal permeability may cause dysregulated immunological responses in the intestinal mucosa that leads to chronic intestinal inflammation. Tight junction proteins contribute to intestinal permeability, and functional abnormality and dislocation of such proteins may cause increased intestinal permeability. We studied the expression of tight junction proteins Rab13, vasodilator-stimulated phosphoprotein (VASP), zonula occludin-1 (ZO-1), and F-actin in the intestinal epithelium of patients with inactive inf...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Ohira M, Oshitani N, Hosomi S, Watanabe K, Yamagami H, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T Tags: Int J Mol Med Source Type: journals
Middle-term expansion of hematopoietic cord blood cells with new human stromal cell line feeder-layers and different cytokine cocktails.Email this article to a colleague.
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Cord blood (CB) is a source of hematopoietic stem cells (HSCs) and is an alternative to bone marrow for allogenic transplantation in patients with hematological disorders. The improvement of HSC in vitro expansion is one of the main challenges in cell therapy. Stromal components and soluble factors, such as cytokines, can be useful to induce in vitro cell expansion. Hence, we investigated whether feeder-layers from new stromal cell lines and different exogenous cytokine cocktails induce HSC expansion in middle-term cultures. CB HSC middle-term expansion was carried out in co-cultures on different feeder-layers exposed ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: De Angeli S, Baiguera S, Del Pup L, Pavan E, Gajo GB, Di Liddo R, Conconi MT, Grandi C, Schiavon O, Parnigotto PP Tags: Int J Mol Med Source Type: journals
The effects of adiponectin on interleukin-6 and MCP-1 secretion in lipopolysaccharide-treated 3T3-L1 adipocytes: role of the NF-kappaB pathway.Email this article to a colleague.
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It was recently suggested that the transcription nuclear factor-kappaB (NF-kappaB) plays an important role in controlling the inflammation and metabolic alterations associated with obesity. In endothelial and monocytic cells, adiponectin acts as a modulator of the inflammatory response, suppressing NF-kappaB activation. The aim of this study was to assess the ability of different forms of adiponectin to modulate the inflammatory response in adipocytes. 3T3-L1 preadipocytes were cultured according to standard conditions. Fully differentiated adipocytes were stimulated with 1 microg/ml lipopolysaccharides (LPS) for 16 h,...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Zoico E, Garbin U, Olioso D, Mazzali G, Fratta Pasini AM, Di Francesco V, Sepe A, Cominacini L, Zamboni M Tags: Int J Mol Med Source Type: journals
Effects of protein kinase Cdelta and phospholipase C-gamma1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death.Email this article to a colleague.
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Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that plays an important role in immune cell migration. It has been reported that chemokines, including MCP-1, are involved in angiogenesis and metastasis. However, the exact role of chemokines in cancer development is still obscure. We investigated the involvement of MCP-1 in taxol-induced breast cancer cell death. The anti-cancer drug taxol induced MCF-7 breast cancer cell death. Treatment with taxol increased the mRNA expression level of MCP-1 in a dose- and time-dependent manner. Up-regulation of MCP-1 by taxol was augmented in cells treated with rottlerin...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Kim YS, An HT, Kim J, Ko J Tags: Int J Mol Med Source Type: journals
The metabolic syndrome of omega3-depleted rats. IV. Intestinal phospholipid omega3 fatty acids.Email this article to a colleague.
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A dietary deprivation in long-chain polyunsaturated omega3 fatty acids initiated in 7-week old normal rats provokes within 3 to 7 months the appearance of several features of the metabolic syndrome. Likewise, within 2 to 4-5 weeks exposure to a flaxseed oil-enriched diet, these anomalies are rapidly corrected. The present study deals with the omega3 fatty acid content of intestinal phospholipids under the same experimental conditions. For the sake of comparison, the control rats were given access during the last 4-5 weeks to either a soybean or flaxseed oil-enriched diet. In control rats, the relative weight content of...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Hacquebard M, Portois L, Malaisse WJ, Carpentier YA Tags: Int J Mol Med Source Type: journals
The metabolic syndrome of omega3-depleted rats. V. Intestinal phospholipid omega6 fatty acids.Email this article to a colleague.
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This study aims mainly at investigating the effects of a dietary deprivation and replenishment of omega3 PUFA upon the phospholipid pattern of omega6 PUFA in the duodenum, jejunum, caecum and colon of rats exposed for 3-7 months to an omega3-depleted diet and then eventually exposed for 2-4 weeks to an omega3-rich diet. In control rats, the relative weight content of all omega6 fatty acids differed in the proximal and distal intestinal segments. In the omega3-depleted rats the C18:2omega6, C20:2omega6 and C20:3omega6 content was decreased whilst that of C20:4omega6 and C22:4omega6 was increased. Significant correlations we...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Hacquebard M, Portois L, Malaisse WJ, Carpentier YA Tags: Int J Mol Med Source Type: journals
Normal table of postembryonic zebrafish development: Staging by externally visible anatomy of the living fishEmail this article to a colleague.
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The zebrafish is a premier model organism yet lacks a system for assigning postembryonic fish to developmental stages. To provide such a staging series, we describe postembryonic changes in several traits that are visible under brightfield illumination or through vital staining and epiflourescent illumination. These include the swim bladder, median and pelvic fins, pigment pattern, scale formation, larval fin fold, and skeleton. We further identify milestones for placing postembryonic fish into discrete stages. We relate these milestones to changes in size and age and show that size is a better indicator of developmental p...
Source: Developmental Dynamics - November 5, 2009 Category: Molecular Biology Authors: David M. Parichy, Michael R. Elizondo, Margaret G. Mills, Tiffany N. Gordon, Raymond E. Engeszer Source Type: journals
Dual functions of Mdt1 in genome maintenance and cell integrity pathways in Saccharomyces cerevisiaeEmail this article to a colleague.
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Recent evidence indicates considerable cross-talk between genome maintenance and cell integrity control pathways. The RNA recognition motif (RRM)- and SQ/TQ cluster domain (SCD)-containing protein Mdt1 is required for repair of 3[prime]-blocked DNA double-strand breaks (DSBs) and efficient recombinational maintenance of telomeres in budding yeast. Here we show that deletion of MDT1 (PIN4/YBL051C) leads to severe synthetic sickness in the absence of the genes for the central cell integrity MAP kinases Bck1 and Slt2/Mpk1. Consistent with a cell integrity function, mdt1[Delta] cells are hypersensitive to the cell wall toxin c...
Source: Yeast - November 5, 2009 Category: Molecular Biology Authors: Ana Traven, Tricia L. Lo, Brietta L. Pike, Helena Friesen, Julie Guzzo, Brenda Andrews, Jörg Heierhorst Source Type: journals
Total synthesis of asperlicin C, circumdatin F, demethylbenzomalvin A, demethoxycircumdatin H, sclerotigenin, and other fused quinazolinonesEmail this article to a colleague.
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Ming-Chung Tseng, Huei-Yun Yang, Yen-Ho Chu
(Paper from Org. Biomol. Chem.)
Ming-Chung Tseng, Org. Biomol. Chem., 2010, DOI: 10.1039/b910545j
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Source: RSC - Organic Biomolecular Chemistry - November 5, 2009 Category: Molecular Biology Authors: Ming-Chung TsengHuei-Yun Yang Yen-Ho Chu Source Type: journals
Facile P,N-heterocycle synthesis via tandem aminomethylation-cyclization of H-phosphinate building blocksEmail this article to a colleague.
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Clemence Queffelec, Jean-Luc Montchamp
(Paper from Org. Biomol. Chem.)
Clemence Queffelec, Org. Biomol. Chem., 2010, DOI: 10.1039/b917428a
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Source: RSC - Organic Biomolecular Chemistry - November 5, 2009 Category: Molecular Biology Authors: Clemence QueffelecJean-Luc Montchamp Source Type: journals
A novel bimodal lipidic contrast agent for cellular labelling and tumour MRIEmail this article to a colleague.
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Nazila Kamaly, Tammy Kalber, Gavin Kenny, Jimmy Bell, Michael Jorgensen, Andrew Miller
(Paper from Org. Biomol. Chem.)
Nazila Kamaly, Org. Biomol. Chem., 2010, DOI: 10.1039/b910561a
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Source: RSC - Organic Biomolecular Chemistry - November 5, 2009 Category: Molecular Biology Authors: Nazila KamalyTammy Kalber Gavin Kenny Jimmy Bell Michael Jorgensen Andrew Miller Source Type: journals
Glycosidase inhibition: assessing mimicry of the transition stateEmail this article to a colleague.
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Tracey M. Gloster, Gideon J. Davies
(Perspective from Org. Biomol. Chem.)
Tracey M. Gloster, Org. Biomol. Chem., 2010, DOI: 10.1039/b915870g
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Source: RSC - Organic Biomolecular Chemistry - November 5, 2009 Category: Molecular Biology Authors: Tracey M. GlosterGideon J. Davies Source Type: journals
Using the ratio of means as the effect size measure in combining results of microarray experimentsEmail this article to a colleague.
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Conclusions:
Our results show that the proposed effect size measure (log ratio of means) has better power to identify differentially expressed genes, and that the detected genes have better performance in predicting cancer outcomes than the commonly used effect size measure, the standardized mean difference (SMD), under both quality-weighted and quality-unweighted data integration frameworks. The new effect size measure and the quality-weighted microarray data integration framework provide efficient ways to combine microarray results.
Source: BMC Systems Biology - Latest articles - November 5, 2009 Category: Molecular Biology Authors: Pingzhao HuCelia GreenwoodJoseph Beyene Source Type: journals
One-carbon metabolism and schizophrenia: current challenges and future directions.Email this article to a colleague.
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Schizophrenia is a heterogeneous disease generally considered to result from a combination of heritable and environmental factors. Although its pathophysiology has not been fully determined, biological studies support the involvement of several possible components including altered DNA methylation, abnormal glutamatergic transmission, altered mitochondrial function, folate deficiency and high maternal homocysteine levels. Although these factors have been explored separately, they all involve one-carbon (C1) metabolism. Furthermore, C1 metabolism is well positioned to integrate gene-environment interactions by influenci...
Source: Trends in Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Krebs MO, Bellon A, Mainguy G, Jay TM, Frieling H Tags: Trends Mol Med Source Type: journals
Muscleblind-like 1 is a negative regulator of TGF-[beta]-dependent epithelial-mesenchymal transition of atrioventricular canal endocardial cellsEmail this article to a colleague.
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The development of the valves and septa of the heart depends on the formation and remodeling of endocardial cushions. Here, we report that the alternative splicing regulator muscleblind-like 1 (MBNL1) exhibits a regionally restricted pattern of expression in canal region endocardium and ventricular myocardium during endocardial cushion development in chicken. Knockdown of MBNL1 in atrioventricular explants leads to a transforming growth factor [beta]-dependent increase in epithelial-mesenchymal transition (EMT) of endocardial cells. This reveals a novel role for MBNL1 during embryonic development, and represents the first ...
Source: Developmental Dynamics - November 4, 2009 Category: Molecular Biology Authors: Natalie A. Vajda, Kyle R. Brimacombe, Kathryn E. LeMasters, Andrea N. Ladd Source Type: journals
Bis-cation salt complexation by meso-octamethylcalix[4]pyrrole: linking complexes in solution and in the solid stateEmail this article to a colleague.
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Claudia Caltagirone, Nathan L. Bill, Dustin E. Gross, Mark E. Light, Jonathan L. Sessler, Philip A. Gale
(Paper from Org. Biomol. Chem.)
Claudia Caltagirone, Org. Biomol. Chem., 2010, DOI: 10.1039/b916113a
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Source: RSC - Organic Biomolecular Chemistry - November 4, 2009 Category: Molecular Biology Authors: Claudia CaltagironeNathan L. Bill Dustin E. Gross Mark E. Light Jonathan L. Sessler Philip A. Gale Source Type: journals
Release of nitrite from the antitubercular nitroimidazole drug PA-824 and analogues upon one-electron reduction in protic, non-aqueous solventEmail this article to a colleague.
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Andrej Maroz, Sujata S. Shinde, Scott G. Franzblau, Zhenkun Ma, William A. Denny, Brian D. Palmer, Robert F. Anderson
(Paper from Org. Biomol. Chem.)
Andrej Maroz, Org. Biomol. Chem., 2010, DOI: 10.1039/b915877d
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Source: RSC - Organic Biomolecular Chemistry - November 4, 2009 Category: Molecular Biology Authors: Andrej MarozSujata S. Shinde Scott G. Franzblau Zhenkun Ma William A. Denny Brian D. Palmer Robert F. Anderson Source Type: journals
Oxidative spirocyclisation routes towards the sawaranospirolides. Synthesis of ent-sawaranospirolides C and DEmail this article to a colleague.
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Jeremy Robertson, Praful T. Chovatia, Thomas G. Fowler, Jonathan M. Withey, Daniel J. Woollaston
(Paper from Org. Biomol. Chem.)
Jeremy Robertson, Org. Biomol. Chem., 2010, DOI: 10.1039/b918091e
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Source: RSC - Organic Biomolecular Chemistry - November 4, 2009 Category: Molecular Biology Authors: Jeremy RobertsonPraful T. Chovatia Thomas G. Fowler Jonathan M. Withey Daniel J. Woollaston Source Type: journals
Collagen type IV and Perlecan exhibit dynamic localization in the Allantoic Core Domain, a putative stem cell niche in the murine allantoisEmail this article to a colleague.
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A body of evidence suggests that the murine allantois contains a stem cell niche, the Allantoic Core Domain (ACD), that may contribute to a variety of allantoic and embryonic cell types. Given that extracellular matrix (ECM) regulates cell fate and function in niches, the allantois was systematically examined for Collagen type IV (ColIV) and Perlecan, both of which are associated with stem cell proliferation and differentiation. Not only was localization of ColIV and Perlecan more widespread during gastrulation than previously reported, but protein localization profiles were particularly robust and dynamic within the allan...
Source: Developmental Dynamics - November 4, 2009 Category: Molecular Biology Authors: Maria M. Mikedis, Karen M. Downs Source Type: journals
Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianusEmail this article to a colleague.
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Kluyveromyces marxianus DMKU3-1042 is a thermotolerant yeast strain suitable for high-temperature ethanol fermentation and genetic engineering with linear DNA. We have developed a highly efficient random gene integration method with a frequency that exceeds 2.5 × 106 transformants/µg linear DNA, a figure comparable to what is observed with autonomously replicating plasmid transformation in Saccharomyces cerevisiae. To establish the mechanism of random integration in DMKU3-1042, we identified and deleted the K. marxianus KU70 gene, which is known to be involved in the non-homologous end-joining (NHEJ) pathway. In yeast la...
Source: Yeast - November 4, 2009 Category: Molecular Biology Authors: Babiker M. A. Abdel-Banat, Sanom Nonklang, Hisashi Hoshida, Rinji Akada Source Type: journals
Computational approaches for the genetic and phenotypic characterization of a Saccharomyces cerevisiae wine yeast collectionEmail this article to a colleague.
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Within this study, we have used a set of computational techniques to relate the genotypes and phenotypes of natural populations of Saccharomyces cerevisiae, using allelic information from 11 microsatellite loci and results from 24 phenotypic tests. A group of 103 strains was obtained from a larger S. cerevisiae winemaking strain collection by clustering with self-organizing maps. These strains were further characterized regarding their allelic combinations for 11 microsatellites and analysed in phenotypic screens that included taxonomic criteria (carbon and nitrogen assimilation tests, growth at different temperatures) and...
Source: Yeast - November 4, 2009 Category: Molecular Biology Authors: R. Franco-Duarte, L. Umek, B. Zupan, D. Schuller Source Type: journals
Overexpression of CsNMAPK in tobacco enhanced seed germination under salt and osmotic stresses.Email this article to a colleague.
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In this research, biological function of CsNMAPK, encoding a mitogen-activated protein kinase of cucumber, was investigated under salt and osmotic stresses. Northern blot analysis showed that the expression of CsNMAPK was induced by salt and osmotic stresses in the cucumber root. In order to determine whether CsNMAPK was involved in plant tolerance to salt and osmotic stresses, transgenic tobacco plants constitutively overexpressing CsNMAPK were generated. Northern and Western blot analysis showed that strong signals were detected in the RNA and protein samples extracted from transgenic lines, whereas no signal was det...
Source: Molecular Biology Reports - November 4, 2009 Category: Molecular Biology Authors: Xu H, Li K, Yang F, Shi Q, Wang X Tags: Mol Biol Rep Source Type: journals
Cloning and characterization of a functional flavanone-3ss-hydroxylase gene from Medicago truncatula.Email this article to a colleague.
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As a key enzyme in the biosynthesis of flavonols, anthocyanidins and proanthocyanidins, flavanone-3ss-hydroxylase (F3H) plays very important roles in plant stress response. A putative flavanone-3ss-hydroxylase gene from Medicago truncatula (MtF3H), a model legume species, was identified from a bio-data analysis platform. It was speculated to be induced by salt stress based on the outcomes of the analysis platform. The complementary DNA (cDNA) consists of 1499 bp with an open reading frame (ORF) of 1098 bp, which encodes a putative protein of 365 amino acids with a molecular weight of about 41.36 kDa and an isoelectric ...
Source: Molecular Biology Reports - November 4, 2009 Category: Molecular Biology Authors: Shen X, Martens S, Chen M, Li D, Dong J, Wang T Tags: Mol Biol Rep Source Type: journals
Gene expression profiling during gland morphogenesis of a mutant and a glandless upland cotton.Email this article to a colleague.
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To identify genes involved in pigment gland morphogenesis in cotton, gene expression was profiled using genechip (Affymetrix) during pigment gland morphogenesis in cotton variety Xiangmian-18, which has glandless seeds but glanded plants, and a glandless line, N5. The results showed that 303 genes were differentially expressed by a factor greater than two during gland morphogenesis; 59% (180) of these genes shared similarity with known genes in GenBank. These genes play roles in defense response, response to oxidative stress, peroxidase activity, and other metabolic pathways. KOBAS (KEGG Orthology-Based Annotation Syst...
Source: Molecular Biology Reports - November 4, 2009 Category: Molecular Biology Authors: Sun Q, Cai Y, Xie Y, Mo J, Yuan Y, Shi Y, Li S, Jiang H, Pan Z, Gao Y, Chen M, He X Tags: Mol Biol Rep Source Type: journals
Molecular characterization and expression analysis of lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP) from pearl oyster Pinctada fucata.Email this article to a colleague.
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The lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP) plays an important function in the innate immune response of invertebrates as a pattern recognition receptor (PRR). Herein, we described the isolation and characterization of pearl oyster Pinctada fucata LGBP (designated as poLGBP). The poLGBP cDNA was 2,075 bp long and consisted of a 5'-untranslated region (UTR) of 18 bp, a 3'-UTR of 299 bp with one cytokine RNA instability motifs (ATTTA), and an open reading frame (ORF) of 1,758 bp encoding a polypeptide of 585 amino acids with an estimated molecular mass of 65.1 kDa and a theoretical isoelectric point...
Source: Molecular Biology Reports - November 4, 2009 Category: Molecular Biology Authors: Zhang D, Ma J, Jiang J, Qiu L, Zhu C, Su T, Li Y, Wu K, Jiang S Tags: Mol Biol Rep Source Type: journals
Analyzing the Effects of Delaying Aster Separation on Furrow Formation during Cytokinesis in the C. elegans Embryo.Email this article to a colleague.
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Monitoring Editor: Yu-Li Wang Signaling by the centrosomal asters and spindle midzone coordinately directs formation of the cytokinetic furrow. Here, we explore the contribution of the asters by analyzing the consequences of altering interaster distance during the first cytokinesis of the C. elegans embryo. Delaying aster separation, using TPXL-1 depletion to shorten the metaphase spindle, leads to a corresponding delay in furrow formation, but results in a single furrow that ingresses at a normal rate. Preventing aster separation, by simultaneously inhibiting TPXL-1 and Galpha signaling-based cortical forces pulling o...
Source: Mol Biol Cell - November 4, 2009 Category: Molecular Biology Authors: Lewellyn L, Dumont J, Desai A, Oegema K Tags: Mol Biol Cell Source Type: journals
NHERF1 Overexpression-dependent Increase of Cytoskeleton Organization Is Fundamental in the Rescue of F508del CFTR in Human Airway CFBE41o- Cells.Email this article to a colleague.
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Monitoring Editor: Keith E. Mostov We have demonstrated that NHERF1 overexpression in CFBE41o- cells induces a significant redistribution of F508del CFTR from the cytoplasm to the apical membrane and rescues CFTR-dependent chloride secretion. Here, we observe that CFBE41o- monolayers displayed substantial disassembly of actin filaments and that overexpression of wt NHERF1 but not NHERF1-DeltaERM increased F-actin assembly and organization. Further, the dominant negative FERM domain of ezrin reversed the wt NHERF1 overexpression-induced increase in both F-actin and CFTR-dependent chloride secretion. wt NHERF1 overexpres...
Source: Mol Biol Cell - November 4, 2009 Category: Molecular Biology Authors: Favia M, Guerra L, Fanelli T, Cardone RA, Monterisi S, Di Sole F, Castellani S, Chen M, Seidler U, Reshkin SJ, Conese M, Casavola V Tags: Mol Biol Cell Source Type: journals
Hydrogen Sulfide Increases HIF-1 Activity Independent of VHL-1 in C. elegans.Email this article to a colleague.
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Monitoring Editor: William P. Tansey Rapid alteration of gene expression in response to environmental changes is essential for normal development and behavior. The transcription factor HIF-1 is well known to respond to alterations in oxygen availability. In nature, low oxygen environments are often found to contain high levels of hydrogen sulfide (H2S). Here we show that C. elegans can have mutually exclusive responses to H2S and hypoxia, both involving HIF-1. Specifically, H2S results in HIF-1 activity throughout the hypodermis while hypoxia causes HIF-1 activity in the gut as judged by a reporter for HIF-1 activity. ...
Source: Mol Biol Cell - November 4, 2009 Category: Molecular Biology Authors: Budde MW, Roth MB Tags: Mol Biol Cell Source Type: journals
Rad4 Regulates Protein Turnover at a Postubiquitylation Step.Email this article to a colleague.
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Monitoring Editor: Jeffrey L. Brodsky The Ub-binding protein Rad23 plays an important role in facilitating the transfer of substrates to the proteasome. However, the mechanism underlying Rad23's function in proteolysis remains unknown. Here, we demonstrate that Rad4, a Rad23-binding protein, also regulates ubiquitylated substrate turnover. Rad4 was previously known only as a key repair factor which directly recognizes DNA damage and initiates DNA repair. Our results, however, reveal a novel function of Rad4. We found that Rad4 and Rad23 share several common substrates. Substrates in rad4Delta cells are ubiquitylated, i...
Source: Mol Biol Cell - November 4, 2009 Category: Molecular Biology Authors: Li Y, Yan J, Kim I, Liu C, Huo K, Rao H Tags: Mol Biol Cell Source Type: journals
Hsp90 Nuclear Accumulation in Quiescence Is Linked to Chaperone Function and Spore Development in Yeast.Email this article to a colleague.
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Monitoring Editor: Benjamin S. Glick Hsp90 operates in the context of a multichaperone complex to promote maturation of nuclear and cytoplasmic clients. We have discovered that Hsp90 and the cochaperone Sba1/p23 accumulate in the nucleus of quiescent S. cerevisiae cells. Hsp90 nuclear accumulation was unaffected in sba1Delta cells, demonstrating that Hsp82 translocates independently of Sba1. Translocation of both chaperones was dependent on the alpha/beta importin SRP1/KAP95. Hsp90 nuclear retention was coincident with glucose exhaustion and appears to be a starvation-specific response, as heat shock or 10% ethanol str...
Source: Mol Biol Cell - November 4, 2009 Category: Molecular Biology Authors: Tapia H, Morano KA Tags: Mol Biol Cell Source Type: journals
Sec3-containing Exocyst Complex Is Required for Desmosome Assembly in Mammalian Epithelial Cells.Email this article to a colleague.
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Monitoring Editor: Patrick J. Brennwald The Exocyst is a conserved multi-subunit complex involved in the docking of post-Golgi transport vesicles to sites of membrane remodeling during cellular processes such as polarization, migration and division. In mammalian epithelial cells, Exocyst complexes are recruited to nascent sites of cell-cell contact in response to E-cadherin-mediated adhesive interactions, and this event is an important early step in the assembly of intercellular junctions. Sec3 has been hypothesized to function as a spatial landmark for the development of polarity in budding yeast, but its role in epit...
Source: Mol Biol Cell - November 4, 2009 Category: Molecular Biology Authors: Andersen NJ, Yeaman C Tags: Mol Biol Cell Source Type: journals
Novel Role of ATPase Subunit C Targeting Peptides Beyond Mitochondrial Protein Import.Email this article to a colleague.
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Monitoring Editor: Thomas D. Fox In mammals, subunit c of the F1F0-ATP synthase has three isoforms (P1, P2, P3). These isoforms differ by their cleavable mitochondrial targeting peptides, while the mature peptides are identical. To investigate this apparent genetic redundancy we knocked down each of the three subunit c isoform by RNA interference in HeLa cells. Silencing any of the subunit c isoforms individually resulted in an ATP synthesis defect, indicating that these isoforms are not functionally redundant. We found that subunit c knock down impaired the structure and function of the mitochondrial respiratory chain...
Source: Mol Biol Cell - November 4, 2009 Category: Molecular Biology Authors: Vives-Bauza C, Magrané J, Andreu AL, Manfredi G Tags: Mol Biol Cell Source Type: journals
Molecular Architecture of Synaptic Actin Cytoskeleton in Hippocampal Neurons Reveals a Mechanism of Dendritic Spine Morphogenesis.Email this article to a colleague.
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Monitoring Editor: Paul Forscher Excitatory synapses in the brain play key roles in learning and memory. The formation and functions of postsynaptic mushroom-shaped structures, dendritic spines, and possibly of presynaptic terminals, rely on actin cytoskeleton remodeling. However, the cytoskeletal architecture of synapses remains unknown hindering the understanding of synapse morphogenesis. Using platinum replica electron microscopy, we characterized the cytoskeletal organization and molecular composition of dendritic spines, their precursors, dendritic filopodia, and presynaptic boutons. A branched actin filament netw...
Source: Mol Biol Cell - November 4, 2009 Category: Molecular Biology Authors: Korobova F, Svitkina T Tags: Mol Biol Cell Source Type: journals
