Molecular Biology
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 7.
Neuronal RNA granule contains ApCPEB1, a novel Aplysia CPEB, in Aplysia sensory neuron.
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The cytoplasmic polyadenylation element (CPE)-binding protein (CPEB) binds to CPE containing mRNAs on their 3' untranslated regions (3'UTRs). This RNA binding protein comes out many important tasks, especially in learning and memory, by modifying the translational efficiency of target mRNAs via poly (A) tailing. Overexpressed CPEB has been reported to induce the formation of stress granules (SGs), a sort of RNA granule in mammalian cell lines. In these days, RNA granule is considered to be a potentially important factor in learning and memory. However, there is no study about RNA granule in Aplysia. To examine whether ...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Chae YS, Lee SH, Cheang YH, Lee N, Rim YS, Jang DJ, Kaang BK Tags: Exp Mol Med Source Type: journals
Induction of TIMP-2 by cholesterol depletion leads to the conversion of pro MMP-2 into active-MMP-2 in human dermal fibroblasts.Email this article to a colleague.
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Cholesterol is one of major components of cell membrane and plays a role in vesicular trafficking and cellular signaling. We investigated the effects of cholesterol on MMP-2 activation in human dermal fibroblasts. We found that TIMP-2 expression and active form MMP-2 (64 kD) were dose-dependently increased by methyl-b-cyclodextrin (MbCD), a cholesterol depletion agent. In contrast, cholesterol depletion-induced TIMP-2 expression and MMP-2 activation were suppressed by cholesterol repletion. Then we investigated the regulatory mechanism of TIMP-2 expression by cholesterol depletion. We found that the phosphorylation of ...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Kim S, Oh JH, Lee Y, Lee J, Cho KH, Chung JH Tags: Exp Mol Med Source Type: journals
Aspirin attenuates the anti-inflammatory effects of theophylline via inhibition of cAMP production in mice with non-eosinophilic asthma.Email this article to a colleague.
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Theophylline is commonly used to treat severe asthma and chronic obstructive pulmonary disease (COPD) characterized by non-eosinophilic inflammation. Acetyl salicylic acid (ASA) is one of the most widely used medications worldwide, but up to 20% of patients with asthma experience aggravated respiratory symptoms after taking ASA. Here we evaluated the adverse effect of ASA on the therapeutic effect of theophylline in mice with non-eosinophilic asthma. A non-eosinophilic asthma mouse model was induced by airway sensitization with lipopolysaccharide (LPS)-containing allergen and then challenged with allergen alone. Therap...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Moon HG, Kim YS, Choi JP, Choi DS, Yoon CM, Jeon SG, Gho YS, Kim YK Tags: Exp Mol Med Source Type: journals
PTH accelerates decompensation following left ventricular hypertrophy.Email this article to a colleague.
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In this study, pressure overload-induced left ventricular hypertrophy (LVH) was induced in mice by transverse aortic banding (TAB) for 2 weeks. We subsequently evaluated the effects of a 2-week treatment with PTH or saline on compensated LVH. After another 4 weeks, the hearts of the mice were analyzed by echocardiography, histology, and molecular biology. Echocardiography showed that hearts of the PTH-treated mice have more severe failing phenotypes than the saline-treated mice following TAB with a greater reduction in fractional shortening and left ventricular posterior wall thickness and with a greater increase in left v...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Cha H, Jeong HJ, Jang SP, Kim JY, Yang DK, Oh JG, Park WJ Tags: Exp Mol Med Source Type: journals
Further understanding of fat biology: Lessons from a fat fly.Email this article to a colleague.
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Obesity is a leading risk factor for insulin resistance, hypertension, hyperlipidemia, and cardiovascular complications, collectively referred to as metabolic diseases. Given the prevalence of obesity and its associated medical problems, new strategies are required to prevent or treat obesity and obesity-related metabolic effects. Here we summarize contributors of obesity, and molecular mechanisms controlling adipogenesis from studies in mammalian systems. We also discuss the possibilities of using Drosophila as a genetic model system to advance our understanding of players in fat biology.
PMID: 19887892 [PubMed - ...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Hong JW, Park KW Tags: Exp Mol Med Source Type: journals
Effect of the membrane permeable cAMP analog, dibutyryl cAMP, on caspase-dependent apoptosis, inflammatory gene expression, and graft survival in acute cardiac allograft rejection.Email this article to a colleague.
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This study was designed to investigate the effects of cAMP on immune regulation and apoptosis during acute rat cardiac allograft rejection. We found that the production of immune markers such as inflammatory cytokines (interlukin-1b, interlukin-6, and tumor necrosis factor-a), inducible nitric oxide synthase (iNOS) expression, and nitric oxide (NO) production, was significantly increased in the blood and transplanted hearts of allograft recipients, but not of isograft controls. These increases were effectively suppressed by the administration of the membrane permeable cAMP analog dibutyryl cAMP (db-cAMP). Administration of...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Lee JY, Kim JH, Chae G, Ha KS, Kwon YG, Kim YM Tags: Exp Mol Med Source Type: journals
Mass spectrometry of the photolysis of sulfonylurea herbicides in prairie waters.Email this article to a colleague.
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This review of mass spectrometry of sulfonylurea herbicides includes a focus on studies relevant to Canadian Prairie waters. Emphasis is given to data gaps in the literature for the rates of photolysis of selected sulfonylurea herbicides in different water matrices. Specifically, results are evaluated for positive ion electrospray tandem mass spectrometry with liquid chromatography separation for the study of the photolysis of chlorsulfuron, tribenuron-methyl, thifensulfuron-methyl, metsulfuron-methyl, and ethametsulfuron-methyl. LC-MS/MS is shown to be the method of choice for the quantification of sulfonylurea herbic...
Source: Mass Spectrometry Reviews - November 4, 2009 Category: Molecular Biology Authors: Headley JV, Du JL, Peru KM, McMartin DW Tags: Mass Spectrom Rev Source Type: journals
Controlled band dispersion for quantitative binding determination and analysis with electrospray ionization-mass spectrometry.Email this article to a colleague.
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This review discusses recent emerging techniques that have been used to couple flow-injection analysis (FIA) and electrospray ionization-mass spectrometry (ESI-MS) for the quantitation of noncovalent binding interactions. Focus is placed predominantly on two such methods. Diffusion-based measurements, developed by Konermann and co-workers, uses controlled-band dispersion prior to ESI-MS to determine diffusion constants and binding constants based on the temporal variation of ligand signal measured in the mass spectrum (an indirect technique). Dynamic titration, developed by Schug and co-workers, is a direct method, whe...
Source: Mass Spectrometry Reviews - November 4, 2009 Category: Molecular Biology Authors: Schug KA, Serrano C, Fryčák P Tags: Mass Spectrom Rev Source Type: journals
The study of ribonucleoproteomics with mass spectrometry (MS)-based technology.Email this article to a colleague.
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PMID: 19890945 [PubMed - as supplied by publisher]
Source: Mass Spectrometry Reviews - November 4, 2009 Category: Molecular Biology Authors: Takahashi N Tags: Mass Spectrom Rev Source Type: journals
The role of mass spectrometry-based metabolomics in medical countermeasures against radiation.Email this article to a colleague.
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Radiation metabolomics can be defined as the global profiling of biological fluids to uncover latent, endogenous small molecules whose concentrations change in a dose-response manner following exposure to ionizing radiation. In response to the potential threat of nuclear or radiological terrorism, the Center for High-Throughput Minimally Invasive Radiation Biodosimetry was established to develop field-deployable biodosimeters based, in part, on rapid analysis by mass spectrometry of readily and easily obtainable biofluids. In this review, we briefly summarize radiation biology and key events related to actual and poten...
Source: Mass Spectrometry Reviews - November 4, 2009 Category: Molecular Biology Authors: Patterson AD, Lanz C, Gonzalez FJ, Idle JR Tags: Mass Spectrom Rev Source Type: journals
Roles of pathologists in molecular targeted cancer therapy Introduction.Email this article to a colleague.
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PMID: 19891708 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - November 4, 2009 Category: Molecular Biology Authors: Osamura RY Tags: J Cell Mol Med Source Type: journals
Pseudoviral immunity - a novel concept for lupus.Email this article to a colleague.
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Systemic lupus erythematosus (SLE or lupus) is a polygenic syndrome of immunity against nuclear autoantigens. Recent data from several fields now suggest 'pseudoviral' immunity as a novel disease concept. Known lupus risk factors commonly compromise those mechanisms that protect chromatin and ribonucleoprotein particles from activating viral nucleic acid sensors. This process activates antigen-presenting cells and induces type I interferons. These central mediators of antiviral immunity have similar proinflammatory roles in lupus, explaining overlapping clinical manifestations, immunopathology and ultrastructural abnor...
Source: Trends in Molecular Medicine - November 4, 2009 Category: Molecular Biology Authors: Anders HJ Tags: Trends Mol Med Source Type: journals
Platelet Derived Growth Factor B and Epithelial Mesenchymal Transition of Peritoneal Mesothelial Cells.Email this article to a colleague.
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Platelet derived growth factor (PDGF) is involved in wound healing in various organ systems. Its potential role in the context of peritoneal injury following long term peritoneal dialysis is unclear. We used an adenovirus expressing the B chain of PDGF (AdPDGF-B) to assess its effect on pro-fibrotic pathways in the peritoneal membrane. To assess the transforming growth factor (TGF) beta independent effects of PDGF, we over-expressed PDGF-B in the peritoneum of either wild-type mice (Smad3(+/+)) or those with a deletion of the TGFbeta signaling protein Smad3 (Smad3(-/-)). PDGF-B induced sustained angiogenesis in both Sm...
Source: Matrix Biology - November 4, 2009 Category: Molecular Biology Authors: Patel P, West-Mays J, Kolb M, Rodrigues JC, Hoff CM, Margetts PJ Tags: Matrix Biol Source Type: journals
Proceedings of the 2009 World Molecular Imaging Congress Montreal, Canada, September 23–26Email this article to a colleague.
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Content Type Journal ArticleDOI 10.1007/s11307-009-0251-y
Journal Molecular Imaging and BiologyOnline ISSN 1860-2002Print ISSN 1536-1632
Source: Molecular Imaging and Biology - November 3, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
Introduction to the Proceedings of the 2nd World Molecular Imaging CongressEmail this article to a colleague.
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Content Type Journal ArticleDOI 10.1007/s11307-009-0253-9
Journal Molecular Imaging and BiologyOnline ISSN 1860-2002Print ISSN 1536-1632
Source: Molecular Imaging and Biology - November 3, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
Author IndexEmail this article to a colleague.
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Content Type Journal ArticleDOI 10.1007/s11307-009-0252-x
Journal Molecular Imaging and BiologyOnline ISSN 1860-2002Print ISSN 1536-1632
Source: Molecular Imaging and Biology - November 3, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals
A novel strategy to inhibit FAK and IGF-1R decreases growth of pancreatic cancer xenograftsEmail this article to a colleague.
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Deregulation of insulin-like growth factor-1 receptor (IGF-1R) and focal adhesion kinase (FAK) signaling pathways plays an important role in cancer cell proliferation and metastasis. In pancreatic cancer cells, the crosstalk and compensatory mechanisms between these two pathways reduce the efficacy of the treatments that target only one of the pathways. Ablation of IGF-1R signaling by siRNA showed minimal effects on the survival and growth of pancreatic cancer cells. An increased activity of FAK pathway was seen in these cells after IGF-1R knockdown. Further inhibition of FAK pathway using Y15 significantly decreased cell ...
Source: Molecular Carcinogenesis - November 3, 2009 Category: Molecular Biology Authors: Donghang Zheng, Vita Golubovskaya, Elena Kurenova, Cheng Wood, Nicole A. Massoll, David Ostrov, William G. Cance, Steven N. Hochwald Source Type: journals
Protein intrinsic disorder and oligomericity in cell signalingEmail this article to a colleague.
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Alexander B. Sigalov
(Review from Mol. BioSyst.)
Alexander B. Sigalov, Mol. BioSyst., 2010, DOI: 10.1039/b916030m
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Source: RSC - Mol. BioSyst. latest articles - November 3, 2009 Category: Molecular Biology Authors: Alexander B. Sigalov Source Type: journals
Functional characterization of two CITED3 homologs (gcCITED3a and gcCITED3b) in the hypoxia-tolerant grass carp, Ctenopharyngodon idellaEmail this article to a colleague.
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Conclusion:
The grass carp gcCITED3a and gcCITED3b genes are differentially expressed and regulated in different fish organs in response to hypoxic stress. This is the first report demonstrating in vivo regulation of two closely-related CITED3 isogenes by HIF-1, as well as CITED3 regulation of HIF-1 transcriptional activity in fish. Overall, our findings suggest that unique molecular mechanisms operate through these two gcCITED3 isoforms that likely play an important regulatory role in the hypoxic response in the grass carp.
Source: BMC Molecular Biology - Latest articles - November 3, 2009 Category: Molecular Biology Authors: Patrick NgSung-Kay ChiuTheresa KwongRichard YuMinnie WongRichard Kong Source Type: journals
Differential role of the menthol-binding residue Y745 in the antagonism of thermally gated TRPM8 channelsEmail this article to a colleague.
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Conclusions:
In summary, we identify structural elements on the TRPM8 channel that are critical for the action of channel antagonists, providing valuable information for the future design of new, specific modulator compounds.
Source: Molecular Pain - November 3, 2009 Category: Molecular Biology Authors: Annika MalkiaMaria PertusaGregorio Fernandez-BallesterAntonio Ferrer-MontielFelix Viana Source Type: journals
Chondroitin sulfate expression is required for cardiac atrioventricular canal formationEmail this article to a colleague.
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Defects in cardiac valvulogenesis are a common cause of congenital heart disease, and the study of this process promises to provide mechanistic insights and lead to novel therapeutics. Normal valve development involves multiple signaling pathways, and recently roles have been identified for extracellular matrix components, including glycosaminoglycans. We, therefore, explored the role of the glycosaminoglycan chondroitin sulfate during zebrafish cardiac development. Beginning at 33 hr, there is a distinct zone of chondroitin sulfate expression in the atrioventricular (AV) boundary, in the cardiac jelly between the endocard...
Source: Developmental Dynamics - November 3, 2009 Category: Molecular Biology Authors: David S. Peal, C. Geoffrey Burns, Calum A. Macrae, David Milan Source Type: journals
Asator, a tau-tubulin kinase homolog in Drosophila localizes to the mitotic spindleEmail this article to a colleague.
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We have used a yeast two-hybrid interaction assay to identify Asator, a tau-tubulin kinase homolog in Drosophila that interacts directly with the spindle matrix protein Megator. Using immunocytochemical labeling by an Asator-specific mAb as well as by transgenic expression of a GFP-labeled Asator construct, we show that Asator is localized to the cytoplasm during interphase but redistributes to the spindle region during mitosis. Determination of transcript levels using qRT-PCR suggested that Asator is expressed throughout development but at relatively low levels. By P-element excision, we generated a null or strong hypomor...
Source: Developmental Dynamics - November 3, 2009 Category: Molecular Biology Authors: Hongying Qi, Changfu Yao, Weili Cai, Jack Girton, Kristen M. Johansen, Jørgen Johansen Source Type: journals
Characterization of molecular markers to assess cardiac cushions formation in XenopusEmail this article to a colleague.
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The valves and septa of the mature heart are derived from the cardiac cushions, which develop from discrete swellings in two regions of developing heart tube: the atrioventricular (AV) canal and the ventricular outflow tract (OFT). In higher vertebrates, three distinct lineages contribute to the heart valves and septa, the endocardium, the myocardium, and the cardiac neural crest that will populate the cardiac jelly of the OFT. Very little is known about cardiac cushions development in amphibians. Here, we describe the expression of eight genes during key stages of cardiac cushion development in Xenopus. Among these genes,...
Source: Developmental Dynamics - November 3, 2009 Category: Molecular Biology Authors: Young-Hoon Lee, Jean-Pierre Saint-Jeannet Source Type: journals
A rapid and efficient method of genotyping zebrafish mutantsEmail this article to a colleague.
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In order to facilitate high throughput genotyping of zebrafish, we have developed a novel technique that uses High Resolution Melting Analysis (HRMA) to distinguish wild-type, heterozygous mutants and homogyzous mutants. This one hour technique removes the need for restriction enzymes and agarose gels. The generated melting curve profiles are sensitive enough to detect non-specific PCR products. We have been able to reliably genotype three classes of mutations in zebrafish, including point mutants, apchu745 (apcmcr), and p53zy7 (p53I166T), a small deletion mutant (bap28y75) and a retroviral insertion mutant (wdr43hi821a). ...
Source: Developmental Dynamics - November 3, 2009 Category: Molecular Biology Authors: John M. Parant, Stephen A. George, Rob Pryor, Carl T. Wittwer, H. Joseph Yost Source Type: journals
Loss of Wnt8b has no overt effect on hippocampus development but leads to altered Wnt gene expression levels in dorsomedial telencephalonEmail this article to a colleague.
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Wnt signalling proteins regulate many aspects of animal development. We have investigated the function of mouse Wnt8b during forebrain development. Wnt8b is expressed in a highly restricted pattern including the prospective hippocampus and hypothalamus. Mutant mice lacking Wnt8b are viable and healthy. The size and morphology of the hippocampus appeared normal in mutant embryos and adults, and we found no evidence of hypothalamic defects in mutants. Wnt8b is also expressed in the neurogenic region of the adult dentate gyrus, however, cell proliferation was unchanged in Wnt8b-/- mutants. Mutant embryos did, however, display...
Source: Developmental Dynamics - November 3, 2009 Category: Molecular Biology Authors: Vassiliki Fotaki, Osmany Larralde, Shaoju Zeng, David McLaughlin, Jennifer Nichols, David J. Price, Thomas Theil, John O. Mason Source Type: journals
Plant chemical defence: a partner control mechanism stabilising plant - seed-eating pollinator mutualisms.Email this article to a colleague.
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Conclusions:
Adonivernith accumulation and larval predation intensity appear to be both the reciprocal cause and effect. Adonivernith not only constitutes an effective chemical means of partner control, but may also play a role in the sympatric diversification of the Chiastocheta genus.
Source: BMC Evolutionary Biology - Latest articles - November 3, 2009 Category: Molecular Biology Authors: Sebastien IbanezChristiane GalletFanny DommangetLaurence Despres Source Type: journals
Acid-sensing ion channels 3: a potential therapeutic target for pain treatment in arthritis.Email this article to a colleague.
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Acid-sensing ion channels 3 (ASIC3) is the most sensitive to such a pH change, predominantly distributed in the sensory peripheral nervous system, and strongly correlated with pain. Recently, there is increasing evidence that ASIC3 may contribute to the pathogenesis of chronic inflammatory pain diseases due to it is predominantly expressed in dorsal root ganglia (DRG) neurons making it a good candidate for a pain sensor. Elevated expression of ASIC3 was found in DRG of rodents with inflamed hind paws. In addition, it has been shown that ASIC3 gene knock-out mice (ASIC3-/-) exhibited no enhanced hyperalgesia in inflamed...
Source: Molecular Biology Reports - November 3, 2009 Category: Molecular Biology Authors: Yuan FL, Chen FH, Lu WG, Li X Tags: Mol Biol Rep Source Type: journals
Identification of Elymus (Triticeae, Poaceae) and its related genera genomes by RFLP analysis of PCR-amplified Adh genes.Email this article to a colleague.
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Elymus L. is the largest genus in Triticeae, containing about 150 species with four recognized genome donors (St, H, P, and W). Traditionally, the genome compound of this genus is identified based on cytological data. Recently, molecular phylogenetic analysis was used to investigate its genomic combination. Here we describe a restriction fragment length polymorphism (RFLP) assay based on digesting alcohol dehydrogenase (Adh) amplicons with two restriction enzyme combinations, EcoRI-HindIII and EcoRI-PstI, which easily can be used to distinguish Elymus and its closely related genera genomes. The method includes only fou...
Source: Molecular Biology Reports - November 3, 2009 Category: Molecular Biology Authors: Liu Q, Zhang N, Li L, Liu J Tags: Mol Biol Rep Source Type: journals
Temporal and spatial expression analysis of PRGL in Gerbera hybrida.Email this article to a colleague.
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GASA-like genes form multigene families in diverse plant species and encode the proteins with a unique cysteine-rich domain (GASA domain). In our previously work, we cloned a GASA-like gene PRGL (Proline-rich GASA-like) from gerbera. Here we report the expression profiles of PRGL and the subcellular localization of PRGL protein. Multiple sequence alignment of the GASA domains indicates that PRGL shows the highest homology to AtPRGL (73.3% of amino acid identity) from Arabidopsis. Phylogenic analysis based on the full amino acid sequences indicates that PRGL and AtPRGL belong to a novel subfamily of GASA proteins. North...
Source: Molecular Biology Reports - November 3, 2009 Category: Molecular Biology Authors: Peng J, Lai L, Wang X Tags: Mol Biol Rep Source Type: journals
Molecular cloning and localization of a novel cotton annexin gene expressed preferentially during fiber development.Email this article to a colleague.
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In this study, a novel cotton annexin gene (designated as GhFAnnx) was isolated from a fiber cDNA library of cotton (Gossypium hirsutum). The full-length cDNA of GhFAnnx comprises an open reading frame of 945 bp that encodes a 314-amino acid protein with a calculated molecular mass of 35.7 kDa and an isoelectric point of 6.49. Genomic GhFAnnx sequences from different cotton species, TM-1, Hai7124 and two diploid progenitor cottons, G. herbaceum (A-genome) and G. raimondii (D-genome) showed that at least two copies of the GhFAnnx gene, each with six exons and five introns in the coding region, were identified in the allotet...
Source: Molecular Biology Reports - November 3, 2009 Category: Molecular Biology Authors: Wang LK, Niu XW, Lv YH, Zhang TZ, Guo WZ Tags: Mol Biol Rep Source Type: journals
Tumor necrosis factor alpha induced by Trypanosoma cruzi infection mediates inflammation and cell death in the liver of infected mice.Email this article to a colleague.
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Trypanosoma cruzi (T. cruzi) infected C57BL/6 mice developed a progressive fatal disease due to an imbalance in the profile of circulating related compounds accompanying infection like tumor necrosis factor alpha (TNFalpha). TNFalpha has been proposed as an important effector molecule in apoptosis. In this work, we evaluate inflammation and the proteins involved in apoptotic process in liver of infected mice and the role of TNFalpha. C57BL6/mice were infected subcutaneously with 100 viable trypomastigotes of Tulahuén strain of T cruzi. One set of these animals were treated with 375mug of antihuman TNFalpha blockin...
Source: Cytokine - November 3, 2009 Category: Molecular Biology Authors: Ronco MT, Francés DE, Ingaramo PI, Quiroga AD, Alvarez ML, Pisani GB, Revelli SS, Carnovale CE Tags: Cytokine Source Type: journals
Transient tropoelastin nanoparticles are early-stage intermediates in the coacervation of human tropoelastin whose aggregation is facilitated by heparan sulfate and heparin decasaccharides.Email this article to a colleague.
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We describe novel 200-300nm spherical particles that serve as intermediates in the formation of the coacervate. Their aggregation gives 800nm to 1microm species. This process is facilitated by heparan sulfate and dermatan sulfate interactions which effectively lower the critical concentration to facilitate this transition. This coacervation process was examined using a panel of heparin chains of various lengths and showed greatest efficacy for the decasaccharide, followed by the octasaccharide, while the hexasaccharide displayed the shortest efficacious length. We propose that these oligosaccharide interactions enable the ...
Source: Matrix Biology - November 3, 2009 Category: Molecular Biology Authors: Tu Y, Weiss AS Tags: Matrix Biol Source Type: journals
Inhibitory role of cAMP on doxorubicin-induced apoptosis in pre-B ALL cells through dephosphorylation of p53 serine residuesEmail this article to a colleague.
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In conclusion, our results suggest that activation of cAMP-signaling system may repress p53-dependent
apoptosis in malignant cells exposed to doxorubicin.
Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10495-009-0417-8Authors
Majid Safa, Iran University of Medical Sciences Department of Hematology, Faculty of Allied Medicine P.O. Box # 14155-6183 Tehran IranAhmad Kazemi, Iran University of Medical Sciences Department of Hematology, Faculty of Allied Medicine P.O. Box # 14155-6183 Tehran IranHamid Zand, Shahid Beheshti University M. C. Department of Basic Medical Sciences, National Nutrition and Food ...
Source: Apoptosis - November 2, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cellsEmail this article to a colleague.
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Abstract A major hallmark of the terminal stages of apoptosis is the internucleosomal DNA fragmentation. The endonuclease responsible
for this type of DNA degradation is the DNA fragmentation factor (DFF). DFF is a complex of the endonuclease DFF40 and its
chaperone/inhibitor, DFF45. In vitro work has shown that histone H1 and HMGB1/2 recruit/target DFF40 to the internucleosomal
linker regions of chromatin and that histone H1 directly interacts with DFF40 conferring DNA binding ability and enhancing
its nuclease activity. The histone H1 family is comprised of many subtypes, which recent work has shown may ha...
Source: Apoptosis - November 2, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Reactive oxygen species mediate thymoquinone-induced apoptosis and activate ERK and JNK signalingEmail this article to a colleague.
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Abstract Thymoquinone (TQ), a component of black seed essential oil, is known to induce apoptotic cell death and oxidative stress,
however, the direct involvement of oxidants in TQ-induced cell death has not been established yet. Here, we show that TQ inhibited
the proliferation of a panel of human colon cancer cells (Caco-2, HCT-116, LoVo, DLD-1 and HT-29), without exhibiting cytotoxicity
to normal human intestinal FHs74Int cells. Further investigation in DLD-1 revealed that apoptotic cell death is the mechanism
for TQ-induced growth inhibition as confirmed by flow cytometry, M30 cytodeath and caspase-3/7 a...
Source: Apoptosis - November 2, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals
Synthesis and conformational analysis of d-2[prime or minute]-deoxy-2[prime or minute],2[prime or minute]-difluoro-4[prime or minute]-dihydro-4[prime or minute]-thionucleosidesEmail this article to a colleague.
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Feng Zheng, Lin Fu, Renxiao Wang, Feng-Ling Qing
(Paper from Org. Biomol. Chem.)
Feng Zheng, Org. Biomol. Chem., 2010, DOI: 10.1039/b914679b
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Source: RSC - Organic Biomolecular Chemistry - November 2, 2009 Category: Molecular Biology Authors: Feng ZhengLin Fu Renxiao Wang Feng-Ling Qing Source Type: journals
Matrix mechanics and receptor-ligand interactions in cell adhesionEmail this article to a colleague.
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Dewi Harjanto, Muhammad H. Zaman
(Emerging Area from Org. Biomol. Chem.)
Dewi Harjanto, Org. Biomol. Chem., 2010, DOI: 10.1039/b913064k
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Source: RSC - Organic Biomolecular Chemistry - November 2, 2009 Category: Molecular Biology Authors: Dewi HarjantoMuhammad H. Zaman Source Type: journals
Inhibition of focal adhesion kinase and src increases detachment and apoptosis in human neuroblastoma cell linesEmail this article to a colleague.
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Neuroblastoma is the most common extracranial solid tumor of childhood. Focal adhesion kinase (FAK) is an intracellular kinase that is overexpressed in a number of human tumors including neuroblastoma, and regulates both cellular adhesion and survival. We have studied the effects of FAK inhibition upon neuroblastoma using adenovirus-containing FAK-CD (AdFAK-CD). Utilizing an isogenic MYCN+/MYCN- neuroblastoma cell line, we found that the MYCN+ cells are more sensitive to FAK inhibition with AdFAK-CD than their MYCN negative counterparts. In addition, we have shown that phosphorylation of Src is increased in the untreated i...
Source: Molecular Carcinogenesis - November 2, 2009 Category: Molecular Biology Authors: Elizabeth A. Beierle, Xiaojie Ma, Angelica Trujillo, Elena V. Kurenova, William G. Cance, Vita M. Golubovskaya Source Type: journals
Experimental and computational studies of enantioseparation of structurally similar chiral compounds on amylose tris(3,5-dimethylphenylcarbamate).Email this article to a colleague.
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The enantioseparation of 14 structurally similar chiral solutes, with one or two chiral centers, are studied for a commercially important polysaccharide-based chiral stationary phase, amylose tris(3,5-dimethylphenylcarbamate) (ADMPC). Among these solutes, only two solutes show significant enantioresolutions of 2 to 2.5 in n-hexane/2-propanol (90/10, v/v) at 298 K. The retention factors of the chiral solutes vary significantly from 0.7 to 7.0, and they are compared with those of simpler nonchiral solutes having similar but fewer functional groups. The sorbent-solute H-bonding interactions between the solute functional g...
Source: Chirality - November 2, 2009 Category: Molecular Biology Authors: Kasat RB, Franses EI, Wang NH Tags: Chirality Source Type: journals
Molecular tweezers for enantiodiscrimination in NMR: Di-(R,R)-1-[10-(1-hydroxy-2,2,2-trifluoroethyl)-9-anthryl]-2,2,2-trifluoroethyl benzenedicarboxylates.Email this article to a colleague.
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A series of new chiral molecular tweezers, di-(R,R)-1-[10-(1-hydroxy-2,2,2-trifluoroethyl)-9-anthryl]-2,2,2-trifluoroethyl phthalate (2), isophthalate (3) and terephthalate (4), were synthesized and their structure studied by NMR and molecular mechanics. Their effectiveness as chiral solvating agents for the determination of the enantiomeric purity of chiral compounds using NMR was demonstrated. Chirality 2010. (c) 2009 Wiley-Liss, Inc.
PMID: 19885822 [PubMed - as supplied by publisher]
Source: Chirality - November 2, 2009 Category: Molecular Biology Authors: Gil S, Palomino-Schätzlein M, Burusco KK, Jaime C, Virgili A Tags: Chirality Source Type: journals
Factors influencing the interconversion of a new class of dibenzodiazepine sulfonamide atropisomers.Email this article to a colleague.
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A novel family of atropisomers based on a conformationally constrained seven membered ring system is investigated using a combination of preparative chiral chromatography, circular dichroism, and other analytical techniques. The influence of structure on the rate of atropisomer interconversion was explored with a series of analogs showing a range of interconversion rates ranging from very fast (undetectable on the HPLC timescale) to very slow (half life of many days). Chirality 2010. (c) 2009 Wiley-Liss, Inc.
PMID: 19885821 [PubMed - as supplied by publisher]
Source: Chirality - November 2, 2009 Category: Molecular Biology Authors: Welch CJ, Gong X, Schafer W, Chobanian H, Lin L, Biba M, Liu P, Guo Y, Beard A Tags: Chirality Source Type: journals
Channel-like crystal structure of cinchoninium L-O-phosphoserine salt dihydrate.Email this article to a colleague.
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Studies on the interactions between L-O- phosphoserine, as one of the simplest fragments of membrane components, and the Cinchona alkaloid cinchonine, in the crystalline state were performed. Cinchoninium L-O-phosposerine salt dihydrate (PhSerCin) crystallizes in a monoclinic crystal system, space group P2(1), with unit cell parameters: a = 8.45400(10) A, b = 7.17100(10) A, c = 20.7760(4) A, alpha = 90 degrees , beta = 98.7830(10) degrees , gamma = 90 degrees , Z = 2. The asymmetric unit consists of the cinchoninium cation linked by hydrogen bonds to a phosphoserine anion and two water molecules. Intermolecular hydroge...
Source: Chirality - November 2, 2009 Category: Molecular Biology Authors: Wesełucha-Birczyńska A, Oleksyn BJ, Wątroba J Tags: Chirality Source Type: journals
New applications and challenges for computational ROA spectroscopy.Email this article to a colleague.
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In this article, applications of quantum chemical methods in calculations of the vibrational Raman optical activity (ROA) spectra are reviewed and new developements are discussed. Modeling of ROA spectra of amino acids and peptides and applications for establishing absolute configuration are briefly outlined. Particular attention is paid to the modeling of solvent effects on ROA spectra, anharmonicity in ROA, resonance and pre-resonance ROA spectra, and ROA spectra of molecules adsorbed on metal surfaces or metal nanoparticles (surface-enhanced Raman optical activity, SEROA). Remaining challenges in computational ROA s...
Source: Chirality - November 2, 2009 Category: Molecular Biology Authors: Pecul M Tags: Chirality Source Type: journals
HPLC-method for determination of permethrin enantiomers using chiral beta-cyclodextrin-based stationary phase.Email this article to a colleague.
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The liquid chromatographic separation of permethrin enantiomers on chiral beta-cyclodextrin-based stationary phase has been investigated. All four enantiomers are obtained by using simple methanol and water mobile phase, under gradient mode. The method was optimized and validated. The relationship between temperature and chromatographic parameters: k' (capacity factor), alpha (separation factor) and Rs (resolution factor) was studied. Van't Hoff's curves for each enantiomer were plotted for temperature range 288-318 K. It was noticed that the response factor ratio of permethrin isomers differ and calculated value is fo...
Source: Chirality - November 2, 2009 Category: Molecular Biology Authors: Shishovska M, Trajkovska V Tags: Chirality Source Type: journals
Application of proteomic marker ensembles to subcellular organelle identification.Email this article to a colleague.
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Compartmentalization of biological processes and the associated cellular components is crucial for cell function. Typically, the location of a component is revealed through a co-localization and/or co-purification with an organelle marker. Therefore, the identification of reliable markers is critical for a thorough understanding of cellular function and dysfunction. We fractionated macrophage-like RAW264.7 cells, both in the resting and in the endotoxin-activated state, into six fractions representing the major organelles/ compartments: nuclei, mitochondria, cytoplasm, endoplasmic reticulum and plasma membrane, as well...
Source: Molecular and Cellular Proteomics : MCP - November 2, 2009 Category: Molecular Biology Authors: Andreyev AY, Shen Z, Guan Z, Ryan A, Fahy E, Subramaniam S, Raetz CR, Briggs S, Dennis EA Tags: Mol Cell Proteomics Source Type: journals
Brain cancer propagating cells: biology, genetics and targeted therapies.Email this article to a colleague.
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Cancer propagating cells (CPCs) within primary central nervous system (CNS) tumors (glioblastoma multiforme (GBM), medulloblastoma (MB) and ependymoma) might be integral to tumor development and perpetuation. These cells, also known as brain cancer propagating cells (BCPCs), have the ability to self-renew and proliferate. BCPCs can initiate new tumors in mice with high efficiency and these exhibit many features that are characteristic of patient's brain tumors. Accumulating evidence suggests that BCPCs might originate from the transformation of neural stem cells (NSCs) and their progenitors. Furthermore, recent studies...
Source: Trends in Molecular Medicine - November 2, 2009 Category: Molecular Biology Authors: Hadjipanayis CG, Van Meir EG Tags: Trends Mol Med Source Type: journals
Impaired IL-7 signaling may explain a case of atypical JAK3-SCID.Email this article to a colleague.
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We report here that the JAK3-E481G mutant transduced IL-2-, IL-4-, IL-15-, and IL-21-induced signals as efficiently as wild-type JAK3. However, this mutant failed to respond to IL-7 by phosphorylating JAK1, JAK3, or STAT5. The other mutant JAK3, JAK3-del482-596, was non-functional. Thus, an impaired IL-7 signal may cause SCID and compromise T-cell differentiation, even if the IL-15 signal is preserved and supports NK-cell development, as in this patient.
PMID: 19889552 [PubMed - as supplied by publisher]
Source: Cytokine - November 2, 2009 Category: Molecular Biology Authors: Li J, Nara H, Rahman M, Juliana FM, Araki A, Asao H Tags: Cytokine Source Type: journals
TGFBR1*6A/9A polymorphism and cancer risk: a meta-analysis of 13,662 cases and 14,147 controls.Email this article to a colleague.
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Published data on the association between TGFBR1*6A/9A polymorphism and cancer risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. A total of 32 studies including 13,662 cases and 14,147 controls were involved in this meta-analysis. Overall, significantly elevated cancer risks were associated with TGFBR1*6A in all genetic models (for allelic effect: OR = 1.11; 95% CI = 1.03-1.21; for 6A/6A vs. 9A/9A: OR = 1.30; 95% CI = 1.01-1.69; for 9A/6A vs. 9A/9A: OR = 1.08; 95% CI = 1.01-1.15; for dominant model: OR = 1.08; 95% CI = 1.02-1.15; for recessive model: OR = 1.2...
Source: Molecular Biology Reports - November 1, 2009 Category: Molecular Biology Authors: Liao RY, Mao C, Qiu LX, Ding H, Chen Q, Pan HF Tags: Mol Biol Rep Source Type: journals
Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopyEmail this article to a colleague.
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Zebrafish and Drosophila are animal models widely used in developmental biology. High-resolution microscopy and live imaging techniques have allowed the investigation of biological processes down to the cellular level in these models. Here, using fluorescence correlation spectroscopy (FCS), we show that even processes on a molecular level can be studied in these embryos. The two animal models provide different advantages and challenges. We first characterize their autofluorescence pattern and determine usable penetration depth for FCS especially in the case of zebrafish, where tissue thickness is an issue. Next, the applic...
Source: Developmental Dynamics - October 31, 2009 Category: Molecular Biology Authors: Xianke Shi, Lin Shin Teo, Xiaotao Pan, Shang-Wei Chong, Rachel Kraut, Vladimir Korzh, Thorsten Wohland Source Type: journals
Phylogenetic analysis of mitochondrial substitution rate variation in the angiosperm tribe SileneaeEmail this article to a colleague.
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Conclusions:
The patterns of phylogenetic divergence within Sileneae suggest enormous variability in plant mitochondrial mutation rates and reveal a complex interaction of gene and species effects. The variation in rates across genomic and phylogenetic scales raises questions about the mechanisms responsible for the evolution of mutation rates in plant mitochondrial genomes.
Source: BMC Evolutionary Biology - Latest articles - October 31, 2009 Category: Molecular Biology Authors: Daniel SloanBengt OxelmanAnja RautenbergDouglas Taylor Source Type: journals
