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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 9.

Metformin attenuates ovarian cancer cell growth in an AMP- kinase dispensable manner.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
ABSTRACT Metformin, the most widely used drug for type 2 diabetes activates AMP-activated protein kinase (AMPK), which regulates cellular energy metabolism. Here, we report that ovarian cell lines VOSE, A2780, CP70, C200, OV202, OVCAR3, SKOV3ip, PE01 and PE04 predominantly express -alpha1, -beta1, -gamma1 and -gamma2 isoforms of AMPK subunits. Our studies show that metformin treatment (1) significantly inhibited proliferation of diverse chemo-responsive and -resistant ovarian cancer cell lines (A2780, CP70, C200, OV202, OVCAR3, SKVO3ip, PE01 and PE04), (2) caused cell cycle arrest accompanied by decreased cyclin D1 and...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Rattan R, Giri S, Hartmann L, Shridhar V Tags: J Cell Mol Med Source Type: journals

Exendin-4 regulates pancreatic ABCA1 transcription via CaMKK/CaMKIV pathway.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
CONCLUSIONS: Activation of the CaMKK/CaMKIV cascade by exendin-4 stimulated ABCA1 gene transcription, indicating that exendin-4 plays an important role in insulin secretion and cholesterol ester content in pancreatic beta cells. PMID: 19874424 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Li J, Murao K, Imachi H, Masugata H, Iwama H, Tada S, Zhang GX, Kobayashi R, Ishida T, Tokumitsu H Tags: J Cell Mol Med Source Type: journals

Involvement of neuropeptide Y and its Y1 and Y5 receptors in maintaining self-renewal and proliferation of human embryonic stem cells.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Abstract Neuropeptide Y (NPY) and NPY receptors are widely expressed in various organs and cell types and have been shown to have pleiotropic functions. However, their presence or role in human embryonic stem cells (hESCs) remains unknown. We now show that undifferentiated hESCs primarily express NPY and its Y1 and Y5 receptors. Inhibition of NPY signaling using either the selective NPY Y1 or Y5 receptor antagonist reduces the maintenance of self-renewal and proliferation of undifferentiated hESCs. We also provide compelling evidence that exogenous NPY supports the long-term growth of undifferentiated hESCs in the abse...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Son MY, Kim MJ, Yu K, Koo DB, Cho YS Tags: J Cell Mol Med Source Type: journals

Serially heterotransplanted human prostate tumors as an experimental model.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Abstract Preclinical research on prostate cancer (PC) therapies uses several models to represent the human disease accurately. A common model uses patient prostate tumor biopsies to develop a cell line by serially passaging and subsequent implantation, in immunodeficient mice. An alternative model is direct implantation of patient prostate tumor biopsies into immunodeficient mice, followed by serial passage in vivo. The purpose of this review is to compile data from the more than 30 years of human PC serial heterotransplantation research. Serially heterotransplanted tumors are characterized by evaluating the histopatho...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Lopez-Barcons LA Tags: J Cell Mol Med Source Type: journals

Phosphatidylinositol 3-kinase interacts with the glucocorticoid receptor upon TLR2 activation.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
ABSTRACT Airway inflammation is a common condition where glucocorticoids (GC) are a well-established therapy. It has been demonstrated that GC stimulate components of innate immunity. Specifically, glucocorticoids up-regulate TLR2 expression and activation upon inflammatory stimuli; however, little is known about the signaling involved in this process. To determine the mechanism by which dexamethasone modulates TLR2-induced cytokine production this signaling pathway was monitored in a lung epithelial cell line exposed to the TLR2 synthetic agonist, Pam(3)-Cys-Ser-Lys(4). These experiments demonstrate that phosphatidyli...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Arancibia S, Benítez D, Núñez LE, Jewell CM, Langjhar P, Candia E, Zapata-Torres G, Cidlowski JA, González MJ, Hermoso MA Tags: J Cell Mol Med Source Type: journals

Isthmin is a novel secreted angiogenesis inhibitor that inhibits tumor growth in mice.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Abstract Antiangiogenesis represents a promising therapeutic strategy for the treatment of various malignances. Isthmin is a gene highly expressed in the isthmus of the midbrain-hindbrain organizer in Xenopus with no known functions. It encodes a secreted 60 kDa protein containing a Thrombospondin Type 1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at the C-terminal. In this work, we demonstrate that isthmin is a novel angiogenesis inhibitor. Recombinant mouse isthmin inhibited endothelial cell (EC) capillary network formation on Matrigel through i...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Xiang W, Ke Z, Zhang Y, Cheng GH, Irwan ID, Sulochana KN, Potturi P, Wang Z, Yang H, Wang J, Zhuo L, Kini RM, Ge R Tags: J Cell Mol Med Source Type: journals

Nuclear applications of inorganic mass spectrometry.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
There are several basic characteristics of mass spectrometry that are not always fully appreciated by the science community. These characteristics include the distinction between relative and absolute isotope abundances, and the influence of isotope fractionation on the accuracy of isotopic measurements. These characteristics can be illustrated in the field of nuclear physics with reference to the measurement of nuclear parameters, which involve the use of enriched isotopes, and to test models of s-, r-, and p-process nucleosynthesis. The power of isotope-dilution mass spectrometry (IDMS) to measure trace elements in p...
Source: Mass Spectrometry Reviews - October 29, 2009 Category: Molecular Biology Authors: De Laeter J Tags: Mass Spectrom Rev Source Type: journals

Serum evaluation of soluble interferon-alpha/beta receptor and high-sensitivity C-reactive protein for diagnosis of the patients with gastrointestinal and hepatobiliary-pancreatic cancer.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Serum soluble interferon-alpha/beta receptor (sIFN-alpha/betaR) and high-sensitivity C-reactive protein (hs-CRP) levels were evaluated in the patients with gastrointestinal and hepatobiliary-pancreatic cancer. We compared the sensitivity and specificity of serum sIFN-alpha/betaR with that of serum hs-CRP and evaluated the two diagnostic parameters in combination. Serum sIFN-alpha/betaR levels were measured in 92 patients and 25 healthy individuals by enzyme-linked immunosorbent assay. The diagnoses were 37 cases of hepatocellular carcinoma, 17 cases of pancreatic cancer, 15 cases of colon cancer, 13 cases of biliary tr...
Source: Cytokine - October 29, 2009 Category: Molecular Biology Authors: Miyake K, Imura S, Nishioka M, Batmunkh E, Sugimoto K, Ohmoto Y, Shimada M Tags: Cytokine Source Type: journals

TNFalpha and TGF-beta1 influence IL-18-induced IFNgamma production through regulation of IL-18 receptor and T-bet expression.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
In this study, we demonstrate that in the dendritic precursor-like cell line KG-1, IFNgamma production induced by IL-18 is potentiated (>5-fold) by TNFalpha and completely suppressed by TGF-beta1. IL-18 stimulation rapidly activates different MAPK signalling pathways but only blocking of p38 activation alleviates IFNgamma production. The mechanism through which TNFalpha enhances IL-18 induced IFNgamma production is by promoting IL-18 receptor alpha-chain expression which results in higher levels of p38 activation and induces expression of T-bet, a transcriptional regulator of the IFNG gene. In contrast, TGF-beta1 rapidl...
Source: Cytokine - October 29, 2009 Category: Molecular Biology Authors: Koutoulaki A, Langley M, Sloan AJ, Aeschlimann D, Wei XQ Tags: Cytokine Source Type: journals

Regulation of memory T cells by gammac cytokines.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
T cells rely on a duality of TCR and gammac cytokine signals for development, activation and peripheral T cell homeostasis. Previous data had suggested that the requirements for CD4 and CD8 memory T cell regulation were qualitatively distinct, but emerging data has shown that the requirements for true antigen specific memory T cells are very similar between these two cell types. This review will focus on contributions made by members of the gammac cytokine family (IL-2, IL-4, IL-7, IL-15 and IL-21) to homeostasis of naïve, memory phenotype and antigen experienced memory T cells. PMID: 19879771 [PubMed - as supplied by publisher]
Source: Cytokine - October 29, 2009 Category: Molecular Biology Authors: Osborne LC, Abraham N Tags: Cytokine Source Type: journals

Unsteady state flow and stagnation in distribution systems affect the biological stability of drinking water.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
The effects of water stagnation and flushing on the biological stability of drinking water were studied by promoting the formation of biofilms under continuous flow (turbulent or laminar) and subsequently subjecting them to unsteady hydraulic situations. Independently of the flow regime under which the biofilm was formed, stagnation promoted bacterial accumulation, either in attached or suspended form, which were carried away in higher numbers when flow was re-started, thereby compromising its biological quality. In all cases, Betaproteobacteria was the dominant phylogenetic group, although Gamma and Alpha subclasses w...
Source: Biofouling - October 28, 2009 Category: Molecular Biology Authors: Manuel CM, Nunes OC, Melo LF Tags: Biofouling Source Type: journals

Synthesis of dihydrodehydrodiconiferyl alcohol: the revised structure of lawsonicinemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Junxiu Meng, Tao Jiang, Huma Aslam Bhatti, Bina S. Siddiqui, Sally Dixon, Jeremy D. Kilburn (Paper from Org. Biomol. Chem.) Junxiu Meng, Org. Biomol. Chem., 2010, DOI: 10.1039/b918179b To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 28, 2009 Category: Molecular Biology Authors: Junxiu MengTao Jiang Huma Aslam Bhatti Bina S. Siddiqui Sally Dixon Jeremy D. Kilburn Source Type: journals

Nucleoside and nucleotide analogues by catalyst free Huisgen nitrile oxide-alkyne 1,3-dipolar cycloadditionemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Virginie Algay, Ishwar Singh, Frances Heaney (Paper from Org. Biomol. Chem.) Virginie Algay, Org. Biomol. Chem., 2010, DOI: 10.1039/b917450h To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 28, 2009 Category: Molecular Biology Authors: Virginie AlgayIshwar Singh Frances Heaney Source Type: journals

Rapid two-step synthesis of drug-like polycyclic substances by sequential multi-catalysis cascade reactionsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Dhevalapally B. Ramachary, Rumpa Mondal, Chintalapudi Venkaiah (Communication from Org. Biomol. Chem.) Dhevalapally B. Ramachary, Org. Biomol. Chem., 2010, DOI: 10.1039/b920152a To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 28, 2009 Category: Molecular Biology Authors: Dhevalapally B. RamacharyRumpa Mondal Chintalapudi Venkaiah Source Type: journals

RAD51 paralogs promote homology-directed repair at diversifying immunoglobulin V regionsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: These results demonstrate direct functions of RAD51D and XRCC2 in immunoglobulin gene conversion, and also suggest that modulation of levels of repair factors may be a useful strategy to promote gene correction in other cell types.
Source: BMC Molecular Biology - Latest articles - October 28, 2009 Category: Molecular Biology Authors: Ellen OrdinarioMunehisa YabukiPriya HandaW. Jason CummingsNancy Maizels Source Type: journals

Thrombospondin-2 and SPARC/osteonectin are critical regulators of bone remodeling.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Thrombospondin-2 (TSP2) and osteonectin/BM-40/SPARC are matricellular proteins that are highly expressed by bone cells. Mice deficient in either of these proteins show phenotypic alterations in the skeleton, and these phenotypes are most pronounced under conditions of altered bone remodeling. For example, TSP2-null mice have higher cortical bone volume and are resistant to bone loss associated with ovariectomy, whereas SPARC-null mice have decreased trabecular bone volume and fail to demonstrate an increase in bone mineral density in response to a bone-anabolic parathyroid hormone treatment regimen. In vitro, marrow st...
Source: Journal of Cell Communication and Signaling - October 28, 2009 Category: Molecular Biology Authors: Delany AM, Hankenson KD Tags: J Cell Commun Signal Source Type: journals

A Novel Mouse HSF3 Has the Potential to Activate Non-classical Heat Shock Genes during Heat Shock.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Monitoring Editor: M. Bishr Omary The heat shock response is characterized by the expression of a set of classical heat shock genes, and is regulated by heat shock transcription factor 1 (HSF1) in mammals. However, comprehensive analyses of gene expression have revealed very large numbers of inducible genes in cells exposed to heat shock. It is believed that HSF1 is required for the heat-inducible expression of these genes although HSF2 and HSF4 modulate some of the gene expression. Here, we identified a novel mouse HSF3 (mHSF3) translocated into the nucleus during heat shock. However, mHSF3 did not activate classical ...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Fujimoto M, Hayashida N, Katoh T, Oshima K, Shinkawa T, Prakasam R, Tan K, Inouye S, Takii R, Nakai A Tags: Mol Biol Cell Source Type: journals

PI(3,4,5)P3 Localization in Recycling Endosomes Is Necessary for AP-1B-dependent Sorting in Polarized Epithelial Cells.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
In conclusion, PI(3,4,5)P3 formation in recycling endosomes is essential for AP-1B function. PMID: 19864464 [PubMed - as supplied by publisher]
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Fields IC, King SM, Shteyn E, Kang RS, Fölsch H Tags: Mol Biol Cell Source Type: journals

Retrograde Neurotrophic Signaling Requires a Protein Interacting with Receptor Tyrosine Kinases via C2H2 Zinc Fingers.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Monitoring Editor: Mark H. Ginsberg Neurotrophins at axonal terminals signal to cell bodies to regulate neuronal development via signaling endosomes containing activated Trk receptor tyrosine kinases and mitogen-activated protein kinases (MAPKs). Requirements for the formation of signaling endosomes remain, however, poorly characterized. Here we show that a novel Trk-interacting protein, NTRAP (neurotrophic factor receptor associated protein), plays a crucial role in this signaling process. NTRAP interacts with the Trk intracellular domain through its C2H2 zinc fingers in a kinase-dependent manner. It is associated wit...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Fu X, Zang K, Zhou Z, Reichardt LF, Xu B Tags: Mol Biol Cell Source Type: journals

The Nup107-160 Nucleoporin Complex Promotes Mitotic Events via Control of the Localisation State of the Chromosome Passenger Complex.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Monitoring Editor: Karsten Weis The human Nup107-160 nucleoporin complex plays a major role in formation of the nuclear pore complex and is localized to kinetochores in mitosis. Here we report that Seh1, a component of the Nup107-160 complex, functions in chromosome alignment and segregation by regulating the centromeric localization of Aurora B and other chromosome passenger complex proteins. Localization of CENP-E is not affected by Seh1 depletion and analysis by electron microscopy showed that microtubule kinetochore attachments are intact. Seh1-depleted cells show impaired Aurora B localization, which results in se...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Platani M, Santarella-Mellwig R, Posch M, Walczak R, Swedlow JR, Mattaj IW Tags: Mol Biol Cell Source Type: journals

Drosophila Lysophospholipid Acyltransferases Are Specifically Required for Germ Cell Development.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Monitoring Editor: Marcos Gonzalez-Gaitan Enzymes of the membrane-bound O-acyltransferase (MBOAT) family add fatty acyl chains to a diverse range of protein and lipid substrates. A chromosomal translocation disrupting human MBOAT1 results in a novel syndrome characterized by male sterility and brachydactyly. We have found that the Drosophila homologues of MBOAT1, Oysgedart (Oys), Nessy (Nes) and Farjavit (Frj), are lysophospholipid acyltransferases. When expressed in yeast, these MBOATs esterify specific lysophospholipids preferentially with unsaturated fatty acids. Generating null mutations for each gene allowed us to...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Steinhauer J, Gijón MA, Riekhof WR, Voelker DR, Murphy RC, Treisman JE Tags: Mol Biol Cell Source Type: journals

Mammalian pre-mRNA 3' End Processing Factor CF Im68 Functions in mRNA Export.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Monitoring Editor: Marvin Wickens Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between mRNA 3' end formation and export, it is not known how these two processes are mechanistically connected. Here, we show that a subunit of the mammalian pre-mRNA 3' end processing complex, CF Im68, stimulates mRNA export. CF Im68 shuttles between the nucleus and the cytoplasm in a transcription-dependent manner and interacts with the mRNA export receptor NXF1/TAP. Consistent with the idea that CF Im68 may act as a novel a...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Ruepp MD, Aringhieri C, Vivarelli S, Cardinale S, Paro S, Schümperli D, Barabino SM Tags: Mol Biol Cell Source Type: journals

Roles of Formin Nodes and Myosin Motor Activity in Mid1p-dependent Contractile-Ring Assembly during Fission Yeast Cytokinesis.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Monitoring Editor: Daniel J. Lew Two prevailing models have emerged to explain the mechanism of contractile-ring assembly during cytokinesis in the fission yeast Schizosaccharomyces pombe: the spot/leading cable model and the search, capture, pull, and release (SCPR) model. We tested some of the basic assumptions of the two models. Monte Carlo simulations of the SCPR model require that the formin Cdc12p is present in >30 nodes from which actin filaments are nucleated and captured by myosin-II in neighboring nodes. The force produced by myosin motors pulls the nodes together to form a compact contractile ring. Live m...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Coffman VC, Nile AH, Lee IJ, Liu H, Wu JQ Tags: Mol Biol Cell Source Type: journals

MICAL-L1 Links EHD1 to Tubular Recycling Endosomes and Regulates Receptor Recycling.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Monitoring Editor: Sandra L. Schmid Endocytic recycling of receptors and lipids occurs via a complex network of tubular and vesicular membranes. EHD1 is a key regulator of endocytosis and associates with tubular membranes to facilitate recycling. Although EHD proteins tubulate membranes in vitro, EHD1 primarily associates with pre-existing tubules in vivo. How EHD1 is recruited to these tubular endosomes remains unclear. We have determined that the Rab8-interacting protein, MICAL-L1, associates with EHD1, with both proteins colocalizing to long tubular membranes, in vitro and in live cells. MICAL-L1 is a largely unchar...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Sharma M, Panapakkam Giridharan SS, Rahajeng J, Naslavsky N, Caplan S Tags: Mol Biol Cell Source Type: journals

The E3 Ubiquitin Ligases Hrd1 and gp78 Bind to and Promote Cholera Toxin Retro-translocation.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Monitoring Editor: Ramanujan S. Hegde To cause disease, cholera toxin (CT) is transported from the cell surface to the endoplasmic reticulum (ER) lumen where the catalytic CTA1 subunit retro-translocates to the cytosol to induce pathological water secretion. Two retro-translocon components are the Derlins and ER-associated multi-spanning E3 ubiquitin ligases including Hrd1 and gp78. We demonstrated previously that Derlin-1 facilitates CTA1 retro-translocation. However, as CTA1 is neither ubiquitinated on lysines nor at its N-terminus, the role of E3 ligases in toxin retro-translocation is unclear. Here, we show that ex...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Bernardi KM, Williams JM, Kikkert M, van Voorden S, Wiertz EJ, Ye Y, Tsai B Tags: Mol Biol Cell Source Type: journals

Role of Rap1B and Tumor Suppressor PTEN in the Negative Regulation of Lysophosphatidic Acid-induced Migration by Isoproterenol in Glioma Cells.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
In this study, we have focused on the negative regulation of lysophosphatidic acid (LPA)-induced migration in glioma cells. Using small-interference RNA and dominant-negative gene strategies in addition to pharmacological tools, we found that isoproterenol (ISO) and sphingosine-1-phosphate (S1P) negatively but differently regulate the LPA-induced migration. ISO-induced suppression of the migration of glioma cells occurs via beta2-adrenergic receptor/cAMP/Epac/Rap1B/inhibition of Rac, whereas S1P has been shown to suppress the migration of the cells through S1P2 receptor/Rho-mediated down-regulation of Rac1. The expression ...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Malchinkhuu E, Sato K, Maehama T, Ishiuchi S, Yoshimoto Y, Mogi C, Kimura T, Kurose H, Tomura H, Okajima F Tags: Mol Biol Cell Source Type: journals

Receptor Type Protein Tyrosine Phosphatase-Kappa (RPTP-{kappa}) Mediates Cross-Talk between TGF-{beta} and EGFR Signaling Pathways in Human Keratinocytes.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
We report here that RPTP-kappa mediates functional integration of EGFR and TGF-beta signaling pathways in human keratinocytes. TGF-beta up-regulates RPTP-kappa mRNA and protein, in a dose and time dependent manner. Induction of RPTP-kappa by TGF-beta significantly decreases basal and EGF-stimulated EGFR tyrosine phosphorylation. shRNA-mediated reduction of TGF-beta-induced RPTP-kappa significantly attenuates the ability of TGF-beta to inhibit proliferation. RPTP-kappa induction is dependent on activation of transcription factors Smad3 and Smad4. Inhibition of TGF-beta receptor kinase completely prevents induction of RPTP-k...
Source: Mol Biol Cell - October 28, 2009 Category: Molecular Biology Authors: Xu Y, Baker D, Quan T, Baldassare JJ, Voorhees JJ, Fisher GJ Tags: Mol Biol Cell Source Type: journals

A proteomic investigation of ligand-dependent HSP90 complexes reveals CHORDC1 as a novel ADP-dependent HSP90 interacting protein.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Structural studies of the chaperone HSP90 have revealed that nucleotide and drug ligands induce several distinct conformational states; however, little is known how these conformations affect interactions with co-chaperones and client proteins. Here we use TAP purification and LC-MS/MS to investigate the proteome-wide effects of ATP, ADP and geldanamycin on the constituents of the human HSP90 interactome. We identified 52 known and novel components of HSP90 complexes that are regulated by these ligands including several co-chaperones. Interestingly, our results also show that geldanamycin treatment causes HSP90 complex...
Source: Molecular and Cellular Proteomics : MCP - October 28, 2009 Category: Molecular Biology Authors: Gano JJ, Simon JA Tags: Mol Cell Proteomics Source Type: journals

Dedifferentiation of human articular chondrocytes is associated with alterations in expression patterns of GDF-5 and its receptors.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Abstract Human articular chondrocytes (HACs) are expanded in monolayer culture in order to obtain sufficient cells for matrix-associated cartilage transplantation (MACT). During this proliferation process, the cells change their shape as well as their expression profile. These changes resemble those that occur during embryogenesis, when the limb anlagen form the interzone that later develops the joint cleft. We analyzed the expression profile of genes that are reportedly important for these changes during embryogenesis within the dedifferentiation process of adult articular chondrocytes. We found GDF-5, BMPR-Ib, and co...
Source: J Cell Mol Med - October 28, 2009 Category: Molecular Biology Authors: Schlegel W, Albrecht C, Eckl P, Freudenthaler H, Berger A, Vécsei V, Marlovits S Tags: J Cell Mol Med Source Type: journals

Wide-spectrum profile of inflammatory mediators in the plasma and scales of patients with psoriatic disease.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Psoriasis is a chronic recurrent inflammatory disorder of the skin. Clinical subtypes include psoriasis vulgaris (PV), psoriatic arthropathy, and erythrodermic psoriasis. Aim of this study was to analyse relevant inflammatory mediators in the plasma of patients with distinct subtypes of active psoriasis, and in the scales of mild-to-moderate PV patients, and correlation to disease severity. Compared to healthy controls (n=10), patients affected by very severe forms of psoriasis (n=30) were characterized by increased plasma levels of IL-4, IL-6, MCP-1, VEGF and in particular PDGFbb. Each group with severe psoriasis had ...
Source: Cytokine - October 28, 2009 Category: Molecular Biology Authors: Deeva I, Mariani S, De Luca C, Pacifico V, Leoni L, Raskovic D, Kharaeva Z, Korkina L, Pastore S Tags: Cytokine Source Type: journals

Topically resolved intramolecular CH-[small pi] interactions in phenylalanine derivativesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
W. Brian Jennings, Noel J. P. McCarthy, Padraig Kelly, John F. Malone (Paper from Org. Biomol. Chem.) W. Brian Jennings, Org. Biomol. Chem., 2009, DOI: 10.1039/b916021n To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 27, 2009 Category: Molecular Biology Authors: W. Brian JenningsNoel J. P. McCarthy Padraig Kelly John F. Malone Source Type: journals

Dendron-anchored organocatalysts: the asymmetric reduction of imines with trichlorosilane, catalysed by an amino acid-derived formamide appended to a dendronemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Marek Figlus, Stuart T. Caldwell, Dawid Walas, Gulen Yesilbag, Graeme Cooke, Pavel Kocovsky, Andrei V. Malkov, Amitav Sanyal (Paper from Org. Biomol. Chem.) Marek Figlus, Org. Biomol. Chem., 2010, DOI: 10.1039/b916601g To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 27, 2009 Category: Molecular Biology Authors: Marek FiglusStuart T. Caldwell Dawid Walas Gulen Yesilbag Graeme Cooke Pavel Kocovsky Andrei V. Malkov Amitav Sanyal Source Type: journals

Estimating the phylogeny and divergence times of primates using a supermatrix approach.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: We present an up-to-date, comprehensive estimate of the structure and tempo of primate evolutionary history. Although considerable gaps remain in our knowledge of the primate phylogeny, increased data sampling, particularly from nuclear loci, will be able to provide further resolution.
Source: BMC Evolutionary Biology - Latest articles - October 27, 2009 Category: Molecular Biology Authors: Helen ChatterjeeSimon HoIan BarnesColin Groves Source Type: journals

Relatedness facilitates cooperation in the subsocial spider, Stegodyphus tentoriicolaemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The combination of our results with those from previous studies indicates that the conflict between individual interests and group interests may be reduced by nepotism and that the latter promote the maintenance of the social community.
Source: BMC Evolutionary Biology - Latest articles - October 27, 2009 Category: Molecular Biology Authors: Jasmin RuchLisa HeinrichTrine BildeJutta Schneider Source Type: journals

Interacting with the biomolecular solvent accessible surface via a haptic feedback deviceemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: By using the software, named HaptiMol ISAS (available from www.haptimol.co.uk), one can explore the accessible surface of biomolecules using a three-dimensional input device to gain insights into the shape and water accessibility of the biomolecular surface that cannot be so easily attained using conventional molecular graphics software.
Source: BMC Structural Biology - Latest articles - October 27, 2009 Category: Molecular Biology Authors: Matthew StocksSteven HaywardStephen Laycock Source Type: journals

Novel inhibitors of the calcineurin/NFATc hub - alternatives to CsA and FK506?email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
The drugs cyclosporine A (CsA) and tacrolimus (FK506) revolutionized organ transplantation. Both compounds are still widely used in the clinic as well as for basic research, even though they have dramatic side effects and modulate other pathways than calcineurin-NFATc, too. To answer the major open question - whether the adverse side effects are secondary to the actions of the drugs on the calcineurin-NFAT pathway - alternative inhibitors were developed. Ideal inhibitors should discriminate between the inhibition of (i) calcineurin and peptidyl-prolyl cis-trans isomerases (PPIases; the matchmaker proteins of CsA and FK506)...
Source: Cell Communication and Signaling - October 27, 2009 Category: Molecular Biology Authors: Matthias SieberRia Baumgrass Source Type: journals

High content screening for inhibitors of protein interactions and post-translational modifications in primary cells by proximity ligation.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
The cost of developing new drugs is a major obstacle for pharmaceutical companies and academia, with many drugs identified in the drug discovery process failing approval for clinical use due to lack of intended effect or because of severe side effects. Since the early 1990s, high-throughput screening of drug compounds has increased enormously in capacity but has not resulted in a higher success rate of the identified drugs. Thus, there is a need for methods that can identify biologically relevant compounds and more accurately predict in vivo effects early in the drug discovery process. To address this, we developed a p...
Source: Molecular and Cellular Proteomics : MCP - October 27, 2009 Category: Molecular Biology Authors: Leuchowius KJ, Jarvius M, Wickström M, Rickardson L, Landegren U, Larsson R, Söderberg O, Fryknäs M, Jarvius J Tags: Mol Cell Proteomics Source Type: journals

Limitations of current therapies to prevent thrombosis: a need for novel strategiesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Jean-Etienne Fabre, Mark E. Gurney (Review from Mol. BioSyst.) Jean-Etienne Fabre, Mol. BioSyst., 2010, DOI: 10.1039/b914375k To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Mol. BioSyst. latest articles - October 26, 2009 Category: Molecular Biology Authors: Jean-Etienne FabreMark E. Gurney Source Type: journals

Clickable fluorescent dyes for multimodal bioorthogonal imagingemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Lun K. Tsou, Mingzi M. Zhang, Howard C. Hang (Communication from Org. Biomol. Chem.) Lun K. Tsou, Org. Biomol. Chem., 2009, DOI: 10.1039/b917119n To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 26, 2009 Category: Molecular Biology Authors: Lun K. TsouMingzi M. Zhang Howard C. Hang Source Type: journals

Hypervalent iodine(iii)-mediated oxidation of aldoximes to N-acetoxy or N-hydroxy amidesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Harisadhan Ghosh, Bhisma K. Patel (Paper from Org. Biomol. Chem.) Harisadhan Ghosh, Org. Biomol. Chem., 2010, DOI: 10.1039/b917096k To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 26, 2009 Category: Molecular Biology Authors: Harisadhan GhoshBhisma K. Patel Source Type: journals

Assessing the cluster glycoside effect during the binding of concanavalin A to mannosylated artificial lipid raftsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Gavin T. Noble, Sabine L. Flitsch, Kwan Ping Liem, Simon J. Webb (Paper from Org. Biomol. Chem.) Gavin T. Noble, Org. Biomol. Chem., 2009, DOI: 10.1039/b910976e To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 26, 2009 Category: Molecular Biology Authors: Gavin T. NobleSabine L. Flitsch Kwan Ping Liem Simon J. Webb Source Type: journals

Highly efficient synthesis of medium-sized lactones via oxidative lactonization: concise total synthesis of isolaurepanemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Makoto Ebine, Yuto Suga, Haruhiko Fuwa, Makoto Sasaki (Communication from Org. Biomol. Chem.) Makoto Ebine, Org. Biomol. Chem., 2010, DOI: 10.1039/b919673k To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 26, 2009 Category: Molecular Biology Authors: Makoto EbineYuto Suga Haruhiko Fuwa Makoto Sasaki Source Type: journals

Sialidase substrate specificity studies using chemoenzymatically synthesized sialosides containing C5-modified sialic acidsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Hongzhi Cao, Yanhong Li, Kam Lau, Saddam Muthana, Hai Yu, Jiansong Cheng, Harshal A. Chokhawala, Go Sugiarto, Lei Zhang, Xi Chen (Paper from Org. Biomol. Chem.) Hongzhi Cao, Org. Biomol. Chem., 2009, DOI: 10.1039/b916305k To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 26, 2009 Category: Molecular Biology Authors: Hongzhi CaoYanhong Li Kam Lau Saddam Muthana Hai Yu Jiansong Cheng Harshal A. Chokhawala Go Sugiarto Lei Zhang Xi Chen Source Type: journals

Total synthesis of (5R,6R,8R,9S)-(-)-5,9Z-indolizidine 221T using sulfinimine-derived N-sulfinyl [small beta]-amino ketonesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Franklin A. Davis, Minsoo Song, Hui Qiu, Jing Chai (Paper from Org. Biomol. Chem.) Franklin A. Davis, Org. Biomol. Chem., 2009, DOI: 10.1039/b915796d To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 26, 2009 Category: Molecular Biology Authors: Franklin A. DavisMinsoo Song Hui Qiu Jing Chai Source Type: journals

Diastereoselective synthesis of (+/-)-1[prime or minute],4[prime or minute]-dimethyluridineemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Guillaume Sautrey, Damien Bourgeois, Christian Perigaud (Paper from Org. Biomol. Chem.) Guillaume Sautrey, Org. Biomol. Chem., 2010, DOI: 10.1039/b912411j To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic Biomolecular Chemistry - October 26, 2009 Category: Molecular Biology Authors: Guillaume SautreyDamien Bourgeois Christian Perigaud Source Type: journals

Partially-supervised protein subclass discovery with simultaneous annotation of functional residuesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The partially-supervised clustering revealed biologically meaningful sub-families even for highly heterogeneous domains and the predicted functional residues provide insights into the basis of the different substrate specificities.
Source: BMC Structural Biology - Latest articles - October 26, 2009 Category: Molecular Biology Authors: Benjamin GeorgiJorg SchultzAlexander Schliep Source Type: journals

A CPTAC inter-laboratory study characterizing a yeast performance standard for benchmarking LC-MS Platform performance.email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
In this study, we describe a standard operating protocol for large-scale production of the yeast performance standard, and offer aliquots to the community through the National Institute of Standards and Technology, where the yeast proteome is under development as a certified reference material to meet the long-term needs of the community. Using a series of metrics that characterize LC-MS performance, we provide a reference dataset demonstrating typical performance of commonly used ion trap instrument platforms in expert laboratories; the results provide a basis for laboratories to benchmark their own performance, to improv...
Source: Molecular and Cellular Proteomics : MCP - October 26, 2009 Category: Molecular Biology Authors: Paulovich AG, Billheimer D, Ham AJ, Vega-Montoto LJ, Rudnick PA, Tabb DL, Wang P, Blackman RK, Bunk DM, Cardasis HL, Clauser KR, Kinsinger CR, Schilling B, Tegeler TJ, Variyath AM, Wang M, Whiteaker JR, Zimmerman LJ, Fenyo D, Carr SA, Fisher SJ, Gibson BW Tags: Mol Cell Proteomics Source Type: journals

Apoptosis in diabetesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Content Type Journal ArticleCategory Diabetes and ApoptosisDOI 10.1007/s10495-009-0419-6Authors Paul A. J. Krijnen, VU Medical Center Department of Pathology De Boelelaan 1117 1081 HV Amsterdam The NetherlandsSuat Simsek, VU Medical Center Department of Internal Medicine Amsterdam The NetherlandsHans W. M. Niessen, VU Medical Center Department of Pathology De Boelelaan 1117 1081 HV Amsterdam The Netherlands Journal ApoptosisOnline ISSN 1573-675XPrint ISSN 1360-8185
Source: Apoptosis - October 24, 2009 Category: Molecular Biology Tags: Apoptosis Source Type: journals

Blood cell generation from the hemangioblastemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Abstract  Understanding how blood cells are generated is important from a biological perspective but also has potential implications in the treatment of blood diseases. Such knowledge could potentially lead to defining new conditions to amplify hematopoietic stem cells (HSCs) or could translate into new methods to produce HSCs, or other types of blood cells, from human embryonic stem cells or induced pluripotent stem cells. Additionally, as most key transcription factors regulating early hematopoietic development have also been implicated in various types of leukemia, understanding their function during norma...
Source: Journal of Molecular Medicine - October 24, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals

Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: The quaternary structure of Nce103 resembles the typical plant type beta-CAs of known structure, with an N-terminal arm indispensable for the enzymatic activity. Comparative structure analysis enables us to draw a possible tunnel for the substrate to access the active site which is located at the bottom of a funnel-shaped substrate pocket.
Source: BMC Structural Biology - Latest articles - October 23, 2009 Category: Molecular Biology Authors: Yan-Bin TengYong-Liang JiangYong-xing HeWei-Wei HeFu-Ming LianYuxing ChenCong-Zhao Zhou Source Type: journals