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Respiratory Medicine

This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 5.

Child uptake of smoking by area across the UK
Smoking is a major public health problem. As smokers age and die prematurely, the tobacco industry must continue to recruit new, young smokers. Survey data indicate that currently in the UK around 207 000 children aged 11–15 start smoking every year. We used local data on adult smoking rates to apportion national data on child smoking uptake to specific areas. The presentation of data for individual local authorities, which now have responsibility for public health, can be used to focus attention locally. For example, this analysis demonstrates that each day, 67 children, more than two classrooms full, start smo...
Source: Thorax - August 11, 2014 Category: Respiratory Medicine Authors: Hopkinson, N. S., Lester-George, A., Ormiston-Smith, N., Cox, A., Arnott, D. Tags: Open access, Child health, Health education, Smoking, Tobacco use, Tobacco use (youth) Chest clinic Source Type: research

The exposome: a new paradigm to study the impact of environment on health
Environmental factors, here taken to include pollutants, lifestyle factors and behaviours, can play an important role in serious, chronic pathologies with large societal and economic costs, including respiratory disease. However, measurement of the environmental component in epidemiological studies has traditionally relied on much more uncertain and incomplete assessments than measurement of the genome. The ‘exposome’ has therefore been proposed as a new paradigm to encompass the totality of human environmental (meaning all non-genetic) exposures from conception onwards, complementing the genome. Evidently, the...
Source: Thorax - August 11, 2014 Category: Respiratory Medicine Authors: Vrijheid, M. Tags: Epidemiologic studies Chest clinic Source Type: research

Fever, thoracic pain, skin lesions and Hampton hump
Case presentation A 56-year-old white woman who had previously been healthy had been admitted to hospital 6 months previously for 2 months due to fever for 1 week associated with an onset of pain in her left hemithorax. She was initially treated for community-acquired pneumonia with a respiratory quinolone and continued to experience pain, which was worse during expiration. She lost 8 kg over the period of her admittance and developed plaque and papules. A chest X-ray showed opacity with fairly precise limits in the lower third of the left hemithorax similar to a Hampton hump, which consists of a shallo...
Source: Thorax - August 11, 2014 Category: Respiratory Medicine Authors: Torres, G. R., Acar, J., Brito, A. C., Pimentel, N., Polisseni, N., Costa, C. H. d., Rufino, R. Tags: Drugs: infectious diseases, Pneumonia (infectious disease), TB and other respiratory infections, Screening (oncology), Pneumonia (respiratory medicine), Radiology (diagnostics), Occupational and environmental medicine Chest clinic Source Type: research

Chronic cough: from a complex dysfunction of the neurological circuit to the production of persistent cough
Chronic cough or cough that lasts more than 8 weeks, once a chest x-ray and spirometry are confirmed normal, is caused by an alteration in a section of the route between peripheral receptors, mainly in the upper and lower airway and oesophagus, spinal cord and the cough centre in the brain stem involving the cortex. These mechanisms of cough have their homology in the circuit of chronic pain, and on that basis, should expand future research of chronic cough. Clinically chronic cough is easy to diagnose by an excessive response or hypertussia to low-intensity stimuli or banal stimuli, which we now call hypersensitivity coug...
Source: Thorax - August 11, 2014 Category: Respiratory Medicine Authors: Pacheco, A. Tags: Radiology (diagnostics) Chest clinic Source Type: research

Pleuropericardial effusion: a diagnostic conundrum
Pleuropericardial effusion as a presenting feature raises the possibility of infectious, neoplastic and connective tissue disorders.1 However, unusual pathophysiological mechanisms may sometimes pose a diagnostic conundrum in certain clinical contexts. Simultaneous pleuropericardial effusion secondary to obstructive uropathy is such a rare scenario with no report mentioned in the published literature. A 62-year-old man presented with progressive dyspnoea and left flank pain for 1 month. A CT scan revealed massive left-sided effusion, mild pericardial effusion (figure 1A,B) and a large staghorn calculi in the left kidn...
Source: Thorax - August 11, 2014 Category: Respiratory Medicine Authors: Kumar, S., Lata, S., Patidar, Y. Tags: Images in Thorax, Journalology, Drugs: infectious diseases, HIV/AIDS, Radiology (diagnostics), Ethics Chest clinic Source Type: research

What's hot that the other lot got
This study provides further evidence for inherited genetic susceptibility to lung cancer, especially in the context of smoking. Mortality reduction in pneumonia treated with azithromycin Elderly patients hospitalised with pneumonia and... (Source: Thorax)
Source: Thorax - August 11, 2014 Category: Respiratory Medicine Authors: Lee, C. Tags: Lung neoplasms, Epidemiologic studies, Drugs: infectious diseases, Pneumonia (infectious disease), TB and other respiratory infections, Lung cancer (oncology), Screening (oncology), Ischaemic heart disease, Lung cancer (respiratory medicine), Pneumonia (r Source Type: research

An animal model for laryngotracheal injuries: An experimental study
ConclusionIn this study, an animal model for inducing and studying laryngotracheal injuries due to endotracheal intubation has been established. This study has the potential applications in research on etiopathogenesis and management of post‐intubation laryngotracheal injuries. Level of EvidenceN/A. Laryngoscope, 2014 (Source: The Laryngoscope)
Source: The Laryngoscope - August 11, 2014 Category: ENT & OMF Authors: Saravanam Prasanna Kumar, Arunchalam Ravikumar, J. Thanka Tags: Laryngology Source Type: research

Outcomes of stem cell transplant patients with acute respiratory failure requiring mechanical ventilation in the United States
& A T Rotta (Source: Bone Marrow Transplantation)
Source: Bone Marrow Transplantation - August 11, 2014 Category: Hematology Authors: V AllareddyA RoyS RampaM K LeeR P NalliahV AllareddyA T Rotta Source Type: research

Cardiorespiratory responses to 6-minute walk test in interstitial lung disease: not always a submaximal test
This study aimed to compare cardiorespiratory responses to 6MWT and cardiopulmonary exercise test (CPET) in people with ILD. Methods: 47 participants with ILD (27 idiopathic pulmonary fibrosis (IPF), mean age 71 (SD 12) years, diffusing capacity for carbon monoxide (TLCO) 49(15) %predicted) undertook CPET and 6MWT on the same day in random order. Oxygen uptake (VO2), ventilation (VE) and carbon dioxide production (VCO2) were assessed during each test using a portable metabolic cart. Results: The VO2peak during the 6MWT was lower than during CPET (1.17(0.27) vs 1.30(0.37) L.min-1, p=0.001), representing an average of 94% (r...
Source: BMC Pulmonary Medicine - Latest articles - August 11, 2014 Category: Respiratory Medicine Authors: Anne HollandLeona DowmanJulio FioreDanny BrazzaleCatherine HillChristine McDonald Source Type: research

C. pneumoniae disrupts eNOS trafficking and impairs NO production in human aortic endothelial cells
In this study, we demonstrate that C. pneumoniae inclusions co‐localize with eNOS, and the microorganism interferes with trafficking of the enzyme from the GA to the plasma membrane in primary human aortic endothelial cells. This mislocation of eNOS results in significant inhibition of NO release by C. pneumoniae infected cells. Furthermore, we show that the distribution of eNOS in C. pneumoniae infected cells is altered due to an intimate association of the Golgi complex with chlamydial inclusions rather than by direct interaction of the enzyme with the chlamydial inclusion membrane. (Source: Cellular Microbiology)
Source: Cellular Microbiology - August 11, 2014 Category: Microbiology Authors: Konrad E. Mueller, Katerina Wolf Tags: Research Article Source Type: research

The role of doppler waveforms in the fetal main pulmonary artery in the prediction of neonatal respiratory distress syndrome
ConclusionsFetal MPA Doppler velocimetry can reliably be obtained throughout gestation. AT and AT/ET ratios of the fetal MPA Doppler waveform may help identifying fetuses at risk of developing neonatal RDS. © 2014 Wiley Periodicals, Inc. J Clin Ultrasound, 2014 (Source: Journal of Clinical Ultrasound)
Source: Journal of Clinical Ultrasound - August 11, 2014 Category: Radiology Authors: Yong Guan, Shengli Li, Guoyang Luo, Chenghong Wang, Errol R. Norwitz, Qian Fu, Xingzhi Tu, Xiaoxian Tian, Jun Zhu Tags: Research Article Source Type: research

Roxithromycin treatment inhibits TGF-ß1-induced activation of ERK and AKT and down-regulation of Caveolin-1 in rat airway smooth muscle cells
Background: Roxithromycin (RXM) has been widely used in asthma treatment; however, the mechanism has not been fully understood. The aim of our study was to investigate the underlying mechanism of RXM treatment in mediating the effect of transforming growth factor (TGF)-?1 on airway smooth muscle cells (ASMCs) proliferation and caveolinn-1 expression. Methods: Firstly, the rat ovalbumin (OVA) model was built according to the previous papers. Rat ASMCs were prepared and cultured, and then TGF-?1 production in ASMCs was measured by enzyme-linked immunosorbent assay (ELISA). Moreover, the proliferation of ASMCs was determined ...
Source: Respiratory Research - August 11, 2014 Category: Respiratory Medicine Authors: Yuanrong DaiFengqin LiLiqin WuRuili WangPing LiSunshun YanHui XuMengling XiaChunxue Bai Source Type: research

HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of Vγ2Vδ2 T cells.
Abstract Whereas infection or immunization of humans/primates with microbes coproducing HMBPP/IPP can remarkably activate Vγ2Vδ2 T cells, in vivo studies have not been done to dissect HMBPP- and IPP-driven expansion, pulmonary trafficking, effector functions, and memory polarization of Vγ2Vδ2 T cells. We define these phosphoantigen-host interplays by comparative immunizations of macaques with the HMBPP/IPP-coproducing Listeria ΔactA prfA* and HMBPP-deficient Listeria ΔactA ΔGCPE: prfA* mutant. The HMBPP-deficient ΔGCPE: mutant shows lower ability to expand Vγ2Vδ2 T cells in vitro than the parental HMBPP-p...
Source: Journal of Leukocyte Biology - August 11, 2014 Category: Hematology Authors: Frencher JT, Shen H, Yan L, Wilson JO, Freitag NE, Rizzo AN, Chen CY, Chen ZW Tags: J Leukoc Biol Source Type: research

Biosignal interpretation I. Advanced methods for studying cardiovascular and respiratory systems.
PMID: 25109424 [PubMed - in process] (Source: Methods of Information in Medicine)
Source: Methods of Information in Medicine - August 11, 2014 Category: Information Technology Authors: Yana K, Cerutti S, Mainardi L, Yamamoto Y Tags: Methods Inf Med Source Type: research

Late Prematurity in Twins: A Polish Multicenter Study.
Abstract The study aimed at investigating the impact of late prematurity (LPT) on neonatal outcome in twins and neonatal morbidity and mortality within LPT with regard to the completed weeks of gestation. The study was conducted in six tertiary obstetric departments from different provinces of Poland (Warsaw, Lublin, Poznan, Wroclaw, Bytom). It included 465 twin deliveries in the above centers in 2012. A comparative analysis of maternal factors, the course of pregnancy and delivery and neonatal outcome between LPT (34 + 0-36 + 6 weeks of gestation) and term groups (completed 37 weeks) was performed. The neonatal ou...
Source: Twin Research and Human Genetics - August 11, 2014 Category: Genetics & Stem Cells Authors: Kosinska-Kaczynska K, Szymusik I, Bomba-Opon D, Madej A, Oleszczuk J, Patro-Malysza J, Marciniak B, Breborowicz G, Drews K, Seremak-Mrozikiewicz A, Szymankiewicz M, Zimmer M, Pomorski M, Olejek A, Slawska H, Wielgos M Tags: Twin Res Hum Genet Source Type: research

[Correction of the broad nasal tip.]
CONCLUSION: The surgery of the nose tip is focused not only with shaping procedures of the middle or lateral crus. The technique reported is very simple and safety for the nasal tip surgery through a modification and/or a reinforcement of the initial structure, maximizing the contact between skin and anatomical component and economizing the interposition of a cartilage graft as far as possible. It is a new concept including respect of the anatomy, and stability, which are the best guarantee for a durable result. PMID: 25125088 [PubMed - as supplied by publisher] (Source: Annales de Chirurgie Plastique et Esthetique)
Source: Annales de Chirurgie Plastique et Esthetique - August 11, 2014 Category: Cosmetic Surgery Authors: Jallut Y Tags: Ann Chir Plast Esthet Source Type: research

[Pulmonary hypertension in chronic respiratory diseases.]
Abstract Pulmonary hypertension is frequent in advanced chronic respiratory diseases, with an estimated prevalence at the time of pulmonary transplantation of 30-50 % in idiopathic pulmonary fibrosis, 30-50 % in chronic obstructive pulmonary disease, 50 % in combined pulmonary fibrosis and emphysema, 75 % in sarcoidosis, and more than 75 % of cases in pulmonary Langerhans cell histiocytosis. Histologic features include varying degrees of pulmonary arterial remodeling (prominent), vascular rarefaction (emphysema), fibrosis or specific involvement of the pulmonary arteries (idiopathic pulmonary fibrosis, sarcoid...
Source: Presse Medicale - August 11, 2014 Category: Journals (General) Authors: Cottin V Tags: Presse Med Source Type: research

[Lactic acidosis in a child with acute severe asthma.]
Abstract Lactic acidosis is a recognized event in adult patients with acute severe asthma (ASA). Only a few cases have been reported in children. Hereinafter is reported the case of a 2-year-old girl hospitalized in the pediatric intensive care unit for ASA, which was treated with high-flow oxygen therapy and intravenous methylprednisolone and salbutamol. During hospitalization, she had metabolic acidosis with a 7.29 pH, a 26mmHg hypocapnia, and a decrease in bicarbonates to 12 mmol/L. The anion gap was increased to 20 mmol/L and lactates to 8 mmol/L. The work-up for a congenital metabolic disease was normal. ...
Source: Archives de Pediatrie - August 11, 2014 Category: Pediatrics Authors: Perrin C, Savy N, Lang M, Caron N, Labbé A Tags: Arch Pediatr Source Type: research

Tailoring of corticosteroids in COPD management.
Abstract This literature review updates the reader on the new studies regarding steroid therapy over the last year in stable COPD and in exacerbations. In stable COPD, we critique the 2011 update and 2013 revision of the GOLD guidelines, discuss why combining inhaled corticosteroids (ICS) with long-acting beta-agonists (LABA) (ICS/LABA) is preferable over LABA alone and review the literature for intraclass differences, finding that the evidence does not clearly support superiority of any particular ICS/LABA. We also address other comparisons against ICS/LABA, including triple therapy. We briefly review which type o...
Source: Respiratory Care - August 10, 2014 Category: Respiratory Medicine Authors: De Coster DA, Jones M Tags: Curr Respir Care Rep Source Type: research

A critical assessment of monitoring practices, patient deterioration, and alarm fatigue on inpatient wards: a review.
Abstract Approximately forty million surgeries take place annually in the United States, many of them requiring overnight or lengthier post operative stays in the over five thousand hospitals that comprise our acute healthcare system. Leading up to this Century, it was common for most hospitalized patients and their families to believe that being surrounded by well-trained nurses and physicians assured their safety. That bubble burst with the Institute of Medicine's 1999 report: To Err Is Human, followed closely by its 2001 report: Crossing the Quality Chasm. This review article discusses unexpected, potentially le...
Source: Patient Safety in Surgery - August 10, 2014 Category: Surgery Authors: Curry JP, Jungquist CR Tags: Patient Saf Surg Source Type: research

Am J Respir Cell Mol Biol; +24 new citations
24 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Respir Cell Mol Biol These pubmed results were generated on 2014/08/09PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - August 10, 2014 Category: Respiratory Medicine Tags: Report Source Type: research

Two pentatricopeptide repeat domain proteins are required for the synthesis of respiratory complex I.
In this study pentatricopeptide repeat (PPR) proteins in filamentous ascomycetes are identified and functionally characterized. PPR proteins, which have in common a degenerated 35 amino acid motif often arranged in multiple tandems, are known to be implicated in various steps of RNA metabolism in mitochondria and chloroplasts. In filamentous ascomycetes we identified a common set of nine PPR proteins. For seven of these proteins, which were not yet characterized, knockout mutants of Neurospora crassa were analyzed. The knockout of three genes appeared to be lethal while four mutants showed different degrees of alterations ...
Source: Current Genetics - August 10, 2014 Category: Genetics & Stem Cells Authors: Solotoff V, Moseler R, Schulte U Tags: Curr Genet Source Type: research

Mitochondrial adaptations evoked with exercise are associated with a reduction in age-induced testicular atrophy in Fischer-344 rats.
Abstract Mitochondrial dysfunction in various tissues has been associated with numerous conditions including aging. In testes, aging induces atrophy and a decline in male reproductive function but the involvement of mitochondria is not clear. The purpose of this study was to examine whether the mitochondrial profile differed with (1) aging, and (2) 10-weeks of treadmill exercise training, in the testes of young (6 month) and old (24 month) Fischer-344 (F344) animals. Old animals exhibited significant atrophy (30 % decline; P < 0.05) in testes compared to young animals. However, relative mitochondrial conten...
Source: Biogerontology - August 10, 2014 Category: Geriatrics Authors: Joseph AM, Nguyen LM, Welter AE, Dominguez JM, Behnke BJ, Adhihetty PJ Tags: Biogerontology Source Type: research

RNA Aptamers for Targeting Mitochondria Using a Mitochondria-Based SELEX Method.
Abstract The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2'-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1. PMID: 25087963 [PubMe...
Source: Biological and Pharmaceutical Bulletin - August 9, 2014 Category: Drugs & Pharmacology Authors: Tawaraya Y, Hyodo M, Ara MN, Yamada Y, Harashima H Tags: Biol Pharm Bull Source Type: research

Suspected cow's milk allergy in everyday general practice: a retrospective cohort study on health care burden and guideline adherence
Conclusion: On a yearly basis seven percent of children visit their GP for suspected CMA. A positive CMA diagnosis was rarely established after adequate implementation and reporting of diagnostics, yet long term dietary measures were prescribed in >70% of patients. There is definitely need for improvement of diagnosing CMA in primary care. (Source: BMC Research Notes)
Source: BMC Research Notes - August 9, 2014 Category: Research Authors: Sharayke van den HoogenAlma van de PolYolanda MeijerJaap ToetCéline van KleiNicolaas de Wit Source Type: research

Monocyte interaction accelerates HCl-induced lung epithelial remodeling
Conclusions: The interaction between epithelial cells and monocytes enhanced epithelial remodelling after initial injury through EMT signalling that is associated with the release of soluble mediators, including IL-8 and PDGF. (Source: BMC Pulmonary Medicine - Latest articles)
Source: BMC Pulmonary Medicine - Latest articles - August 9, 2014 Category: Respiratory Medicine Authors: Qiuhua ChenAlice LuoHaibo QiuBing HanBruce KoArthur SlutskyHaibo Zhang Source Type: research

Health-related quality of life and chronic obstructive pulmonary disease in early stages - longitudinal results from the population-based KORA cohort in a working age population
Conclusion: Despite small HRQL differences between COPD patients in early disease stages and controls and small changes over ten years, our results indicate that it is important to prevent subjects with airflow limitation from progression to higher grades. Awareness of HRQL impairments in early stages is important for offering early interventions in order to maintain high HRQL in COPD patients. (Source: BMC Pulmonary Medicine - Latest articles)
Source: BMC Pulmonary Medicine - Latest articles - August 9, 2014 Category: Respiratory Medicine Authors: Margarethe WackerMatthias HungerStefan KarraschJoachim HeinrichAnnette PetersHolger SchulzRolf Holle Source Type: research

Pharmacoepidemiology of common colds and upper respiratory tract infections in children and adolescents in Germany
Conclusions: The use of medicines to treat CCs or URTIs is widespread among children and adolescents in Germany. Thus, longitudinal studies should investigate the risks associated with this drug use. Differences in socio-demographic variables regarding exposure to antibiotic use indicate that there could be an implausible prescribing behavior among physicians in Germany. (Source: BMC Clinical Pharmacology)
Source: BMC Clinical Pharmacology - August 9, 2014 Category: Drugs & Pharmacology Authors: Nathalie EckelGiselle SarganasIngrid-Katharina WolfHildtraud Knopf Source Type: research

[Comment] Positive end-expiratory pressure in surgery: good or bad?
A key challenge in perioperative care is to reduce postoperative morbidity. Patients who develop postoperative pulmonary complications but survive to leave hospital, typically have reduced functional independence and shortened long-term survival. Mechanical ventilation is an example of how we might shift from treatment to prevention of postoperative complications. Stimulated by findings in patients with acute respiratory distress syndrome, a multicentre randomised trial (IMPROVE) of intraoperative lung-protective ventilation was undertaken, using a strategy that consisted of a low tidal volume, moderate positive end-expira...
Source: LANCET - August 9, 2014 Category: Journals (General) Authors: Emmanuel Futier Tags: Comment Source Type: research

Health-related quality of life and chronic obstructive pulmonary disease in early stages ¿ longitudinal results from the population-based KORA cohort in a working age population
Conclusion: Despite small HRQL differences between COPD patients in early disease stages and controls and small changes over ten years, our results indicate that it is important to prevent subjects with airflow limitation from progression to higher grades. Awareness of HRQL impairments in early stages is important for offering early interventions in order to maintain high HRQL in COPD patients. (Source: BMC Pulmonary Medicine - Latest articles)
Source: BMC Pulmonary Medicine - Latest articles - August 9, 2014 Category: Respiratory Medicine Authors: Margarethe WackerMatthias HungerStefan KarraschJoachim HeinrichAnnette PetersHolger SchulzRolf Holle Source Type: research

Adolescent with Fever, Hypotension, and Respiratory Distress
A previously healthy 15-year-old White girl presented to the pediatric clinic with chest pain and difficulty breathing. During the preceding week she had experienced a fever, sore throat, nonproductive cough, significant fatigue, and multiple episodes of nonbloody, nonbilious vomiting. She reported markedly reduced oral intake and decreasing urine output. She appeared fatigued and in severe respiratory distress. (Source: Journal of Pediatric Health Care)
Source: Journal of Pediatric Health Care - August 9, 2014 Category: Pediatrics Authors: Sharda Udassi, Sanjeev Y. Tuli, Beverly P. Giordano, Jai Udassi Source Type: research

Biologic Comparison of Inhaled Insulin Formulations: Exubera(TM) and Novel Spray-Dried Engineered Particles of Dextran-10.
Abstract Inhaled peptides and proteins have promise for respiratory and systemic disease treatment. Engineered spray-dried powder formulations have been shown to stabilize peptides and proteins and optimize aerosol properties for pulmonary delivery. The current study was undertaken to investigate the in vitro and in vivo inhalation performance of a model spray-dried powder of insulin and dextran 10 in comparison to Exubera™. Dextrans are a class of glucans that are generally recognized as safe with optimum glass transition temperatures well suited for spray drying. A 70% insulin particle loading was prepared by f...
Source: AAPS PharmSciTech - August 9, 2014 Category: Drugs & Pharmacology Authors: Kuehl PJ, Cherrington A, Dobry DE, Edgerton D, Friesen DT, Hobbs C, Leach CL, Murri B, Neal D, Lyon DK, Vodak DT, Reed MD Tags: AAPS PharmSciTech Source Type: research

Establishment and application of a multiplex PCR for rapid and simultaneous detection of six viruses in swine.
Abstract A multiplex PCR assay was developed and evaluated subsequently for its effectiveness in simultaneously detecting mixed viral infections of swine. Specific primers were designed and used for testing the six swine viruses: three DNA viruses, including pseudorabies virus (PRV), porcine parvovirus (PPV), and porcine circovirus type 2 (PCV2); three common RNA viruses, including porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), and Japanese encephalitis virus (JEV). This technique has shown to be highly sensitive in that the minimum detection amounts of nucleic acid...
Source: Journal of Virological Methods - August 9, 2014 Category: Virology Authors: Zeng ZY, Liu ZJ, Wang WC, Tang DY, Liang HY, Liu Z Tags: J Virol Methods Source Type: research

Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages.
In this study, we found that PEDV infected not only the small intestines, but also the respiratory tract. Infection and replication of PEDV in the respiratory tract from naturally PEDV-infected piglets was examined by reverse transcription polymerase chain reaction, immunohistochemistry, and virus re-isolation. Our observations were confirmed by experimental inoculation, and we found that PEDV infection in the respiratory tract was specifically associated with alveolar macrophages in the lung. Vero cell-adapted PEDV was able to replicate in both primary alveolar macrophages and continuous porcine alveolar macrophage cells....
Source: Virus Research - August 9, 2014 Category: Virology Authors: Park JE, Shin HJ Tags: Virus Res Source Type: research

The SARS Coronavirus 3a protein binds calcium in its cytoplasmic domain.
Abstract The Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) is a positive stranded RNA virus with ∼ 30kb genome. Among all open reading frames (orfs) of this virus, the orf3a is largest, and encodes a protein of 274 amino acids, named as 3a protein. Sequence analysis suggests that the orf3a aligned to one calcium pump present in Plasmodium falciparum and the enzyme glutamine synthetase found in Leptospira interrogans. This sequence similarity was found to be limited only to amino acid residues 209-264 which form the cytoplasmic domain of the orf3a. Furthermore, this region was predicted to be involved i...
Source: Virus Research - August 9, 2014 Category: Virology Authors: Minakshi R, Padhan K, SafikurRehman, Hassan MI, Ahmad F Tags: Virus Res Source Type: research

High cell density culture with S. cerevisiae CEN.PK113-5D for IL-1β production: optimization, modeling, and physiological aspects.
Abstract Saccharomyces cerevisiae CEN.PK113-5D, a strain auxotrophic for uracil belonging to the CEN.PK family of the yeast S. cerevisiae, was cultured in aerated fed-batch reactor as such and once transformed to express human interleukin-1β (IL-1β), aiming at obtaining high cell densities and optimizing IL-1β production. Three different exponentially increasing glucose feeding profiles were tested, all of them "in theory" promoting respiratory metabolism to obtain high biomass/product yield. A non-structured non-segregated model was developed to describe the performance of S. cerevisiae CEN.PK113-5D during the ...
Source: Bioprocess and Biosystems Engineering - August 9, 2014 Category: Biomedical Engineering Authors: Landi C, Paciello L, de Alteriis E, Brambilla L, Parascandola P Tags: Bioprocess Biosyst Eng Source Type: research

Immunoprophylaxis against respiratory syncytial virus with palvizumab: what is new?
(Source: Revista Paulista de Pediatria)
Source: Revista Paulista de Pediatria - August 8, 2014 Category: Pediatrics Source Type: research

Respiratory infections in children up to two years of age on prophylaxis with palivizumab
CONCLUSIONS: During the prophylaxis period, low frequency of respiratory syncytial virus infections and low rates of hospitalization were observed, suggesting the benefit of palivizumab prophylaxis. OBJETIVO: Identificar los virus implicados en los cuadros de infecciones agudas de trato respiratorio y analizar las tasas de internación y de óbito en niños sometidos a la profilaxis con palivizumabe. MÉTODOS: Cohorte prospectiva con 198 niños con menos de un año nacidas antes de 29 semanas de edad gestacional y niños con menos de dos años con cardiopatía hemodinámicamente inestable o enfermedad pulmonar crónica q...
Source: Revista Paulista de Pediatria - August 8, 2014 Category: Pediatrics Source Type: research

Assessment of respiratory muscle strength in children according to the classification of body mass index
CONCLUSIONS: Overweight and obese children showed lower MIP values, compared to eutrophic ones. The findings point to the influence of anthropometric variables on respiratory muscle strength in children. OBJETIVO: Evaluar y comparar la fuerza muscular respiratoria de escolares eutróficos, con sobrepeso y obesos, así como identificar variables antropométricas y respiratorias que se relacionen con los resultados. MÉTODOS: Estudio transversal con escolares sanos de 7 a 9 años, divididos en tres grupos: Eutróficos, Sobrepeso y Obesos. Se aplicó el cuestionario del International Study of Asthma and Allergies in Childhoo...
Source: Revista Paulista de Pediatria - August 8, 2014 Category: Pediatrics Source Type: research

Inflammatory and Cell-Mediated Immune Responses in the Respiratory Tract of Chickens to Infection with Avian Infectious Bronchitis Virus
Viral Immunology , Vol. 0, No. 0. (Source: Viral Immunology)
Source: Viral Immunology - August 8, 2014 Category: Allergy & Immunology Tags: article Source Type: research

Capturing the Efficiency of Vibrating Mesh Nebulizers: Minimizing Upper Airway Deposition
Journal of Aerosol Medicine and Pulmonary Drug Delivery , Vol. 0, No. 0. (Source: Journal of Aerosol Medicine and Pulmonary Drug Delivery)
Source: Journal of Aerosol Medicine and Pulmonary Drug Delivery - August 8, 2014 Category: Respiratory Medicine Tags: article Source Type: research

Abstract Almost a decade after the devastating SARS epidemic, which infected more than 8,000 patients and causing more than 900 deaths, the outbreak of a novel coronavirus in the middle east poses a major threat to human health.(1) The middle east respiratory syndrome coronavirus (MERS-CoV) belongs to the genus Betacoronavirus, which was frst isolated form Saudi Arabia in September 2012, in two patients with severe pneumonia. PMID: 25095856 [PubMed - in process] (Source: J Contemp Dent Pract)
Source: J Contemp Dent Pract - August 8, 2014 Category: Dentistry Authors: Sukumaran A, Patil S Tags: J Contemp Dent Pract Source Type: research

A colorimetric immunoassay of respiratory syncytial virus by using gold nanoparticles/graphene oxide hybrids with mercury-enhanced peroxidase-like activity
Chem. Commun., 2014, Accepted ManuscriptDOI: 10.1039/C4CC05155F, CommunicationLei Zhan, Chun Mei Li, Wen Bi Wu, Cheng Zhi HuangA novel colorimetric immunoassay of respiratory syncytial virus (RSV), one of the leading causes of severe lower respiratory tract infections in all age groups, has been proposed with high sensitivity...The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Chem. Commun. latest articles)
Source: RSC - Chem. Commun. latest articles - August 8, 2014 Category: Chemistry Authors: Lei Zhan Source Type: research

Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Conclusions: These results suggest that the RT-LAMP assay described here is a useful tool for the diagnosis and epidemiologic surveillance of human MERS-CoV infections. (Source: Epidemiologic Perspectives and Innovations)
Source: Epidemiologic Perspectives and Innovations - August 8, 2014 Category: Epidemiology Authors: Kazuya ShiratoTakuya YanoSyouhei SenbaShigehiro AkachiTakashi KobayashiTakamichi NishinakaTsugunori NotomiShutoku Matsuyama Source Type: research