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Immune balance at the foeto-maternal interface as the fulcrum of reproductive success
Abstract: Viviparity has many evolutionary advantages but brings with it the problem of the semi-allogeneic foetus having to coexist with the mother for the duration of pregnancy. In species with haemochorial placentation this problem is particularly evident as foetal trophoblast cells are extensively intermingled with maternal tissue and are directly exposed to maternal blood. Fascinating adaptations on both the foetal and maternal side have allowed for this interaction to be re-directed away from an immune rejection response not only towards immunotolerance, but in fact towards actively supporting reproductive success. R...
Source: Journal of Reproductive Immunology - February 22, 2013 Category: Allergy & Immunology Authors: Myriam Hemberger Source Type: research

Pregnancy-acquired fetal progenitor cells
Abstract: The transfer and persistence of fetal progenitor cells into the mother throughout pregnancy has sparked considerable interest as a trafficking stem cell and immunological phenomenon. Indeed, the intriguing longevity of semi-allogeneic fetal microchimeric cells (FMC) in parous women raises questions over their potential clinical implications. FMC have been associated with both immune-modulatory roles and participation in maternal tissue repair. Although their influence on maternal health is as yet unresolved, FMC selectively home to damaged maternal tissues and often integrate, adopting site-appropriate phenotypes...
Source: Journal of Reproductive Immunology - February 22, 2013 Category: Allergy & Immunology Authors: E. Seppanen, N.M. Fisk, K. Khosrotehrani Source Type: research

Osteoblasts Off
I am pleased to announce we now have unlabeled and FITC-labeled Osteoblasts. These are differentiated from our Umbilical Cord Blood derived Human Mesenchymal Stem Cells. They are designed for:Osteogenesis/Bone Formation StudiesCompound/Small Molecule TestingGene Expression AnalysisImages: (A) Human cord-blood MSCs were expanded in low-serum MSC-GroTM to confluence as shown here. (B) were differentiated in osteogenic MSC-GroTM. Early stage osteoblasts are shown here; the arrow shows early formation of mineralized matrix. (C)&(D) Mature osteoblasts stained positive for Alizarin red. Phase contrast image at 200 x, sc...
Source: Neuromics - February 22, 2013 Category: Neuroscience Tags: Osteoblast Cells Osteogenesis hMSCs Osteoblasts human Mesenchymal Stem Cell Based Assays Human Mesenchymal Stem Cells Source Type: news

IBN Creates Unlimited Source of Human Kidney Cells
Researchers at the Institute of Bioengineering and Nanotechnology (IBN) have successfully generated human kidney cells from human embryonic stem cells in vitro.02/22/2013
Source: Kidney Cancer Association - February 22, 2013 Category: Cancer & Oncology Source Type: news

Development: Old Stem Cells Rejuvenated
Hematopoietic stem cells (HSCs) in the bone marrow give rise to all cells of the blood, but with age, this capacity decreases. Sirtuin 3 (SIRT3) is a deactylase expressed in … [Read more]
Source: Editors' Choice - February 22, 2013 Category: Science Authors: Stewart Wills (mailto:swills at aaas.org) Source Type: research

Editors' Choice
Climate Science: Modern Warming by Proxy | Economics: Risky Fishing | Neuroscience: Tuning Brain Blood Flow | Development: Old Stem Cells Rejuvenated | Applied Physics: A Quick Freeze | Physics: Voltage-Controlled Magnetism | Immunology: Regulating TLR Traffic Flow
Source: Science: Current Issue - February 22, 2013 Category: Science Authors: Stewart Wills (mailto:swills at aaas.org) Source Type: research

A prospective randomized toxicity study to compare reduced‐intensity and myeloablative conditioning in patients with myeloid leukemia undergoing allogeneic haematopoietic stem cell transplantation
ConclusionRIC had several advantages compared to MAC, including less mucositis, less haemorrhagic cystitis, faster platelet engraftment, the need for fewer transfusions and less TPN, and fewer CMV infections. Both regimens were tolerated and TRM was low.© 2013 The Association for the Publication of the Journal of Internal Medicine
Source: Journal of Internal Medicine - February 22, 2013 Category: Internal Medicine Authors: Olle Ringdén, Tom Erkers, Johan Aschan, Karin Garming‐Legert, Katarina Blanc, Hans Hägglund, Brigitta Omazic, Petter Svenberg, Göran Dahllöf, Jonas Mattsson, Per Ljungman, Mats Remberger Tags: Original Source Type: research

The Effects of Substrate Elastic Modulus on Neural Precursor Cell Behavior.
In conclusion, limitations in NPC growth, proliferation, and neuronal differentiation were encountered when substrate elasticity was not within normal spinal cord tissue elasticity ranges. These studies elucidate the effect injury mediated mechanical changes may have on tissue repair by stem cells. Furthermore, this information can be applied to the development of future neuroregenerative biomaterials for spinal cord repair. PMID: 23429962 [PubMed - as supplied by publisher]
Source: Annals of Biomedical Engineering - February 22, 2013 Category: Biomedical Engineering Authors: Previtera ML, Hui M, Verma D, Shahin AJ, Schloss R, Langrana NA Tags: Ann Biomed Eng Source Type: research

Interferon‐γ capture T cell therapy for persistent Adenoviraemia following allogeneic haematopoietic stem cell transplantation
Source: British Journal of Haematology - February 22, 2013 Category: Hematology Authors: Waseem Qasim, Kimberly Gilmour, Hong Zhan, Sophie Derniame, Anne Marie McNicol, Winnie Ip, Prashant Hiwarkar, Paul Veys, Hubert B. Gaspar Tags: Correspondence Source Type: research

Nanomechanics controls neuronal precursors adhesion and differentiation
Abstract The ability to control the differentiation of stem cells into specific neuronal types has a tremendous potential for the treatment of neurodegenerative diseases. In vitro neuronal differentiation can be guided by the interplay of biochemical and biophysical cues. Different strategies to increase the differentiation yield have been proposed, focusing everything on substrate topography, or, alternatively on substrate stiffness. Both strategies demonstrated an improvement of the cellular response. However it was often impossible to separate the topographical and the mechanical contributions. Here we investigate the r...
Source: Biotechnology and Bioengineering - February 22, 2013 Category: Biomedical Science Authors: Elisa Migliorini, Jelena Ban, Gianluca Grenci, Laura Andolfi, Alessandro Pozzato, Massimo Tormen, Vincent Torre, Marco Lazzarino Tags: Article Source Type: research

Immobilized Heparan Sulfate for Embryonic Stem Cell Culture [Glycobiology and Extracellular Matrices]
As our understanding of what guides the behavior of multi- and pluripotent stem cells deepens, so too does our ability to utilize certain cues to manipulate their behavior and maximize their therapeutic potential. Engineered, biologically functionalized materials have the capacity to influence stem cell behavior through a powerful combination of biological, mechanical, and topographical cues. Here, we present the development of a novel electrospun scaffold, functionalized with glycosaminoglycans (GAGs) ionically immobilized onto the fiber surface. Bound GAGs retained the ability to interact with GAG-binding molecules and, ...
Source: Journal of Biological Chemistry - February 22, 2013 Category: Chemistry Authors: Meade, K. A., White, K. J., Pickford, C. E., Holley, R. J., Marson, A., Tillotson, D., van Kuppevelt, T. H., Whittle, J. D., Day, A. J., Merry, C. L. R. Tags: Glycobiology and Extracellular Matrices Source Type: research

SON Regulates GATA-2 via MicroRNA [RNA]
SON is a DNA- and RNA-binding protein localized in nuclear speckles. Although its function in RNA splicing for effective cell cycle progression and genome stability was recently unveiled, other mechanisms of SON functions remain unexplored. Here, we report that SON regulates GATA-2, a key transcription factor involved in hematopoietic stem cell maintenance and differentiation. SON is highly expressed in undifferentiated hematopoietic stem/progenitor cells and leukemic blasts. SON knockdown leads to significant depletion of GATA-2 protein with marginal down-regulation of GATA-2 mRNA. We show that miR-27a is up-regulated upo...
Source: Journal of Biological Chemistry - February 22, 2013 Category: Chemistry Authors: Ahn, E. E.-Y., Higashi, T., Yan, M., Matsuura, S., Hickey, C. J., Lo, M.-C., Shia, W.-J., DeKelver, R. C., Zhang, D.-E. Tags: Gene Regulation Source Type: research

REPORT: Derivation of hiPSCs from Patients with MODY [Developmental Biology]
Maturity onset diabetes of the young (MODY) is an autosomal dominant disease. Despite extensive research, the mechanism by which a mutant MODY gene results in monogenic diabetes is not yet clear due to the inaccessibility of patient samples. Induced pluripotency and directed differentiation toward the pancreatic lineage are now viable and attractive methods to uncover the molecular mechanisms underlying MODY. Here we report, for the first time, the derivation of human induced pluripotent stem cells (hiPSCs) from patients with five types of MODY: MODY1 (HNF4A), MODY2 (GCK), MODY3 (HNF1A), MODY5 (HNF1B), and MODY8 (CEL) with...
Source: Journal of Biological Chemistry - February 22, 2013 Category: Chemistry Authors: Teo, A. K. K., Windmueller, R., Johansson, B. B., Dirice, E., Njolstad, P. R., Tjora, E., Raeder, H., Kulkarni, R. N. Tags: Reports Source Type: research

STK40 Contributes to Lung Maturation [Gene Regulation]
STK40 is a putative serine/threonine kinase and was shown to induce extraembryonic endoderm differentiation from mouse embryonic stem cells. However, little is known about its physiological function in vivo. Here, we generate Stk40 knock-out mice and demonstrate that loss of the Stk40 gene causes neonatal lethality at birth. Further examination reveals that the respiratory distress and atelectasis occur in the homozygous mutants. The maturation of lung and alveolar epithelium is delayed in the mutant, as indicated by narrowed air spaces, thickened interstitial septa, and increased glycogen content in the lungs of Stk40−/...
Source: Journal of Biological Chemistry - February 22, 2013 Category: Chemistry Authors: Yu, H., He, K., Li, L., Sun, L., Tang, F., Li, R., Ning, W., Jin, Y. Tags: Developmental Biology Source Type: research

Nuclear transferred embryonic stem cells for analysis of B1 B-lymphocyte development
The transfer of nuclei of fully differentiated cells into enucleated oocytes is a well-recognized method for the generation of embryonic stem (ES) cells. Here, we demonstrate that nuclear transferred ES (NT-ES) cells can be established with high efficiency using innate-like B lymphocytes as donor cells. We established two mouse lines carrying rearranged immunoglobulin heavy and light chains using NT-ES cells containing nuclei from peritoneal cavity B1 cells. Analysis of B1 clone lines revealed that the B1-cell generation critically depends on the interaction between antigen (possibly self-antigen) and surface immunoglobuli...
Source: International Immunology - February 22, 2013 Category: Allergy & Immunology Authors: Takase, M., Iida, R., Maruya, M., Sakaue-Sawano, A., Miyawaki, A., Wakayama, T., Nishigami, S., Fagarasan, S., Kanagawa, O. Tags: Original Research Source Type: research

JCI early table of contents for Feb. 22, 2013
(Journal of Clinical Investigation) This release contains summaries, links to PDFs, and contact information for the following newsworthy papers to be published online, Feb. 22, 2013, in the JCI: How to mend a broken heart: advances in parthenogenic stem cells; Pulmonary fibrosis: between a ROCK and a hard place; Epigenetic alterations reprogram pancreatic cells to secrete insulin; Brain "clean-up crew" captured by MRI; and many more.
Source: EurekAlert! - Medicine and Health - February 22, 2013 Category: Global & Universal Source Type: news

How to mend a broken heart: Advances in parthenogenic stem cells
(Journal of Clinical Investigation) In this issue of the Journal of Clinical Investigation, Wolfram Zimmerman and colleagues at Georg-August-Universität Göttingen in Göttingen, Germany, demonstrated that cells from the parthenogenote function as embryonic stem cells and maintain the capacity to develop into different types of tissue.
Source: EurekAlert! - Medicine and Health - February 22, 2013 Category: Global & Universal Source Type: news

Video: Stem Cells for Leukemia: New Hope From Newborns
SEATTLE, Wash. (Ivanhoe Newswire) -- One person in the US is diagnosed with blood cancer every four minutes. Chemo and a bone marrow transplant helps fight off the disease, but thousands of those diagnosed each year cannot find a matching donor and surviving without one is almost impossible, but now a solution may be coming from a surprising place.
Source: Medical Headlines From Ivanhoe.com - February 22, 2013 Category: Consumer Health News Source Type: news

Microfluidic devices for the isolation of circulating rare cells: a focus on affinity‐based, dielectrophoresis and hydrophoresis
Abstract Circulating rare cells have attracted interest because they can be good indicators of various types of diseases. For example, enumeration of circulating tumor cells (CTCs) is used for cancer diagnosis and prognosis, while DNA analysis or enumeration of nucleated red blood cells (NRBCs) is useful for prenatal diagnosis or hypoxic anemia, and that of circulating stem cells to diagnose cancer metastasis. Isolation of these cells and their downstream analyses can provide significant information such as the origin and characteristics of a disease. Novel approaches based on microfluidics have many advantages, including ...
Source: Electrophoresis - February 22, 2013 Category: Biochemistry Authors: Kyung‐A. Hyun, Hyo‐Il Jung Tags: Review Article Source Type: research

Gene Therapy Shows Early Promise for Heart Failure
Injecting a protein known to recruit stem cells may help repair damaged heart muscle, researcher says
Source: WebMD Health - February 21, 2013 Category: Consumer Health News Source Type: news

Risk Adopted Allogeneic Hematopoietic Stem Cell Transplantation Using a Reduced Intensity Regimen for Children with Thalassemia Major
ConclusionImplementing a risk‐adopted therapy in patient with thalassemia in Jordan can result in an excellent thalassemia free and OS, especially in those at highest risk. Pediatr Blood Cancer
Source: Pediatric Blood and Cancer - February 21, 2013 Category: Cancer & Oncology Authors: Ayad Ahmed Hussein, Abdulhadi Al‐Zaben, Lubna Ghatasheh, Abeer Natsheh, Tuka Hammada, Fawzi Abdel‐Rahman, Husam Abu‐Jazar, Shanta Sharma, Rula Najjar, Haydar Frangoul Tags: Research Article Source Type: research

Is extramedullary relapse of acute myeloid leukemia after allogeneic hematopoietic stem cell transplantation associated with improved survival?
ConclusionsOur results suggest that EM relapse post‐HSCT has similar outcomes to BM relapses treated in a similar way. Furthermore, choice of therapy at relapse appears related to the time post‐HSCT that the relapse occurs, with BM relapses occurring significantly earlier post‐HSCT than relapses at EM sites.
Source: Asia-Pacific Journal of Clinical Oncology - February 21, 2013 Category: Cancer & Oncology Authors: Cameron Curley, Simon Durrant, Glen A Kennedy Tags: ORIGINAL ARTICLE Source Type: research

Gene Therapy Shows Early Promise for Heart Failure
Injecting a protein known to recruit stem cells may help repair damaged heart muscle, researcher says Source: HealthDay Related MedlinePlus Pages: Genes and Gene Therapy, Heart Failure
Source: MedlinePlus Health News - February 21, 2013 Category: Consumer Health News Source Type: news

One Man's Reluctant Tour For Adult Stem Cells
Michael Phelan is the CEO of SevOne. My Forbes colleague Tomio Geron recently wrote about his fast-growing IT company and Phelan contributed a guest post earlier this year at Eric Savitz's CIO Network.
Source: Forbes.com Healthcare News - February 21, 2013 Category: Pharmaceuticals Authors: John Farrell Source Type: news

Gentium Provides Update on the Review of Defibrotide Marketing Authorization
VILLA GUARDIA, Italy, Feb. 21, 2013 -- (Healthcare Sales & Marketing Network) -- Gentium S.p.A. (GENT) (the "Company") announced today that, the Company had presented an oral explanation at the European Medicines Agency's ("EMA") Commit... Biopharmaceuticals, RegulatoryGentium, Defibrotide, hepatic veno-occlusive disease, stem cell transplantation
Source: HSMN NewsFeed - February 21, 2013 Category: Pharmaceuticals Source Type: news

What is the clinical value of cancer stem cell markers in gliomas?
In conclusion we find that CD133, nestin, CD133/nestin, podoplanin, Musashi-1 and Musashi-1/MIB1 are the most promising markers for future investigation. Evaluation in larger cohorts with known clinical data and known status of important biomarkers like MGMT and IDH1 is necessary to reveal their full clinical potential. PMID: 23412423 [PubMed - in process]
Source: International Journal of Clinical and Experimental Pathology - February 21, 2013 Category: Pathology Authors: Dahlrot RH, Hermansen SK, Hansen S, Kristensen BW Tags: Int J Clin Exp Pathol Source Type: research

New Guidelines on Testing Kids DNA-the Cliff s Notes Version
Exomes are big news. Sequencing of the protein-encoding part of the genome is increasingly solving medical mysteries in children. It began with Nicholas Volker and his recovery from a devastating gastrointestinal disease with a stem cell transplant once his exome sequence revealed his problem.And my recent Medscape assignments reveal the trend: 7 of 12 kids' exomes leading to diagnosis at Duke University (from May 10, 2012); whole genomes of 5 infants from the neonatal intensive care unit at Children's Mercy Hospital and Clinics in Kansas City, Missouri (from October 3), in just 50 hours each, focusing on 600 single-gene d...
Source: Scientific American - Official RSS Feed - February 21, 2013 Category: Science Tags: Health,More Science Source Type: research

Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering.
Abstract We introduce a novel Bombyx mori silk-based composite material developed for bone tissue engineering. Three-dimensional scaffolds were fabricated by embedding of natural degummed silk fibers in a matrix of regenerated fibroin, followed by freeze-drying. Different ratios of fibers to fibroin were investigated with respect to their influence on mechanical and biological properties. For all scaffold types, an interconnected porous structure suitable for cell penetration was proven by scanning electron microscopy. Compressive tests, carried out in static and cyclic mode under dry as well as wet conditions, rev...
Source: Cell Research - February 21, 2013 Category: Cytology Authors: Mobini S, Hoyer B, Solati-Hashjin M, Lode A, Nosoudi N, Samadikuchaksaraei A, Gelinsky M Tags: J Biomed Mater Res A Source Type: research

Phenotypic Evolution In A Case of Peripheral T‐Cell Lymphoma Suggests The Presence of Tumor Heterogeneity
Abstract Peripheral T‐cell lymphoma (PTCL), not otherwise specified (NOS), represents a heterogeneous group of nodal and extranodal lymphomas that express a variety of T‐cell antigens indicative of mature T‐cell lineage. Most cases of PTCL express CD4 and lack CD8 expression and have a T‐helper immunophenotype. Although the immunophenotype of PTCL is usually stable over time, immunophenotypic switch or evolution from T‐helper to T‐suppressor or vice versa has been rarely reported. Herein, we report a patient who presented with nasal PTCL, NOS, that was CD8+ and negative for Epstein‐Barr virus, with concurrent...
Source: Journal of Cutaneous Pathology - February 21, 2013 Category: Pathology Authors: Alyaa Al‐Ibraheemi, Rashmi Kanagal‐Shamanna, Keyur P. Patel, Sergej N. Konoplev, Pramod Metha, L. Jeffrey Medeiros, Roberto N. Miranda Tags: Case Report Source Type: research

Human testicular peritubular cells - more than meets the eye.
Abstract In healthy men several layers of inconspicuously flat cells and extracellular matrix (ECM) proteins build the wall of the seminiferous tubules. The cells of this wall, peritubular cells, are not well characterized. They are smooth-muscle-like and contractile and transport immotile sperm, a function important for male fertility. However, their full functional importance, especially their potential contribution to the paracrine regulation of the male gonad, is unknown. In men with impaired spermatogenesis the architecture of the tubular wall is frequently altered. Deposits of ECM and morphological changes of...
Source: Reproduction - February 21, 2013 Category: Reproduction Medicine Authors: Mayerhofer A Tags: Reproduction Source Type: research

Evaluation of 16 SNPs allele-specific to quantify post hSCT chimerism by SYBR green-based qRT-PCR
The importance of monitoring post haematopoietic stem cell transplantation (hSCT) chimerism has been defined in numerous publications. Single-nucleotide polymorphisms (SNPs) are molecular markers that vary significantly among different populations. Allied to a very sensible technique, SNP assays seem to be very sensitive (0.001%) when post hSCT chimerism is measured. However, well known SNP frequencies are limited to certain populations, mainly in countries where there is a high level of diversity in its population, therefore restricting their use worldwide. Amplification by SYBR green based quantitative real time PCR of e...
Source: Journal of Clinical Pathology - February 21, 2013 Category: Pathology Authors: Almeida, C. A. C., Dreyfuss, J. L., Azevedo-Shimmoto, M. M., Figueiredo, M. S., de Oliveira, J. S. R. Tags: Molecular genetics, Immunology (including allergy) Original article Source Type: research

Cancer stem cells markers CD44, CD24 and ALDH1 in breast cancer special histological types
Conclusions The expression distribution of breast CSC markers is largely dependent on histological type. Interestingly, within the distinct SHT, medullary and metaplastic carcinomas are the two types highly associated with high-grade carcinomas, basal-like and claudin-low molecular subtypes, and to the CSC phenotype CD44+/CD24–/low/ALDH1+.
Source: Journal of Clinical Pathology - February 21, 2013 Category: Pathology Authors: Beca, F. F. d., Caetano, P., Gerhard, R., Alvarenga, C. A., Gomes, M., Paredes, J., Schmitt, F. Tags: Immunology (including allergy), Breast cancer Original article Source Type: research

Association between pterygium and plica semilunaris morphology
Ophthalmic pterygium remains an “enigma” despite numerous proposed pathogenetic theories, such as the molecular genetic alterations of stem cells located at the sclerocorneal limbus1. The nasal conjunctiva may be particularly exposed to environmental irritants due to the presence of the “lacrimal lake”, formed in the area of the plica semilunaris2. An edematous enlargement of the latter may result from chronic ocular surface inflammation3. A previous study has reported that nasal pterygia are associated with plica semilunaris ectopia, causing “with‐the‐rule” corneal astigmatism due to the exertion of tracti...
Source: Clinical and Experimental Ophthalmology - February 21, 2013 Category: Opthalmology Authors: Efstathios T Detorakis, Aikaterini Halkia, Vassiliki Tsakalaki, Dimitrios A Spandidos Tags: Letter to the Editor Source Type: research

SOX2 Co-Occupies Distal Enhancer Elements with Distinct POU Factors in ESCs and NPCs to Specify Cell State
by Michael A. Lodato, Christopher W. Ng, Joseph A. Wamstad, Albert W. Cheng, Kevin K. Thai, Ernest Fraenkel, Rudolf Jaenisch, Laurie A. Boyer SOX2 is a master regulator of both pluripotent embryonic stem cells (ESCs) and multipotent neural progenitor cells (NPCs); however, we currently lack a detailed understanding of how SOX2 controls these distinct stem cell populations. Here we show by genome-wide analysis that, while SOX2 bound to a distinct set of gene promoters in ESCs and NPCs, the majority of regions coincided with unique distal enhancer elements, important cis-acting regulators of tissue-specific gene express...
Source: PLoS Genetics - February 21, 2013 Category: Genetics & Stem Cells Authors: Michael A. Lodato et al. Source Type: research

A human neuronal model of Niemann Pick C disease developed from stem cells isolated from patient's skin
In conclusion, we generated a human neuronal model of NPC disease through the induction of differentiation of stem cells obtained from patient's easily accessible sources. The strategy described here may be applied to easily generate human neuronal models of other neurodegenerative diseases.
Source: Orphanet Journal of Rare Diseases - February 21, 2013 Category: Internal Medicine Authors: Natascha BergaminAndrea DardisAntonio BeltramiDaniela CesselliSilvia RigoStefania ZampieriRossana DomenisBruno BembiCarlo Beltrami Source Type: research

Anti-KIT designer T cells for the treatment of gastrointestinal stromal tumor
Conclusions: We have constructed a novel anti-KIT CIR for production of dTc that possess specific activity against KIT+ GIST in vitro and in vivo. Further studies are warranted to evaluate the therapeutic potential and safety of anti-KIT dTc.
Source: Journal of Translational Medicine - February 21, 2013 Category: Research Authors: Steven KatzRachel BurgaSeema NaheedLauren LicataMitchell ThornDoreen OsgoodCang NguyenN EspatJonathan FletcherRichard Junghans Source Type: research

Predictors of unsuccessful mobilization with granulocyte colony‐stimulating factor alone in patients undergoing autologous hematopoietic stem cell transplantation
Mobilization of hematopoietic stem cells is achieved with hematopoietic growth factors with or without chemotherapy or other agents. Although studies comparing granulocyte colony‐stimulating factor (G‐CSF) alone to combined regimens demonstrate an increase in stem cell yield in the latter, mobilization with G‐CSF alone is still effective and has been widely practiced. We conducted a retrospective cohort study of consecutive patients at our institution who underwent at least one mobilization attempt with G‐CSF between January 2000 and December 2008 to identify the proportion of patients failing one or more mobilizat...
Source: Journal of Clinical Apheresis - February 21, 2013 Category: Hematology Authors: Signy Chow, Alejandro Lazo‐Langner, George Ormond, Kang Howson‐Jan, Anargyros Xenocostas Tags: Research Article Source Type: research

An algorithm for utilizing peripheral blood CD34 count as a predictor of the need for plerixafor in autologous stem cell mobilization—cost‐effectiveness analysis
This study retrospectively evaluated the effectiveness of utilizing a peripheral blood CD34+ stem cell count (PBCD34) ≤8/µL on day 4 of GCSF‐based AHSC mobilization as a threshold for plerixafor administration, and compared the efficacy of collection and cost analysis using historical controls. All patients in the study cohort reached their CD34+ targets in ≤3 collections. Significantly more patients who received plerixafor + GCSF versus GCSF alone reached their CD34+ target in one collection (P = 0.045); however, there were no significant differences in the number of collections or in cumulative product yie...
Source: Journal of Clinical Apheresis - February 21, 2013 Category: Hematology Authors: Ghada A. Abusin, Rolla F. Abu‐Arja, Roger D. Gingrich, Margarida D. Silverman, Gideon K. D. Zamba, Annette J. Schlueter Tags: Research Article Source Type: research

Race and ethnicity in decisions about unrelated hematopoietic stem cell donation
Key Points Across racial/ethnic groups, ambivalence is strongly associated with HSC donation decisions.
Source: Blood - February 21, 2013 Category: Hematology Authors: Switzer, G. E., Bruce, J. G., Myaskovsky, L., DiMartini, A., Shellmer, D., Confer, D. L., Abress, L. K., King, R. J., Harnaha, A. G., Ohngemach, S., Dew, M. A. Tags: Transplantation, Free Research Articles Source Type: research

Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment
Key Points NOD-specific Sirpa polymorphism is the genetic determinant of highly efficient xenograft activity in NOD-based immunodeficient mouse models.
Source: Blood - February 21, 2013 Category: Hematology Authors: Yamauchi, T., Takenaka, K., Urata, S., Shima, T., Kikushige, Y., Tokuyama, T., Iwamoto, C., Nishihara, M., Iwasaki, H., Miyamoto, T., Honma, N., Nakao, M., Matozaki, T., Akashi, K. Tags: Hematopoiesis and Stem Cells Source Type: research

In vivo generation of transplantable human hematopoietic cells from induced pluripotent stem cells
Key Points Human hematopoietic cells develop within human iPSC-derived teratomas in immunodeficient mice. Co-transplantation of OP9 stromal cells along with human iPSCs increases hematopoietic specification within teratomas.
Source: Blood - February 21, 2013 Category: Hematology Authors: Amabile, G., Welner, R. S., Nombela-Arrieta, C., D'Alise, A. M., Di Ruscio, A., Ebralidze, A. K., Kraytsberg, Y., Ye, M., Kocher, O., Neuberg, D. S., Khrapko, K., Silberstein, L. E., Tenen, D. G. Tags: Hematopoiesis and Stem Cells, Plenary Papers Source Type: research

Tu-mor(e) blood cells from human pluripotent stem cells
Source: Blood - February 21, 2013 Category: Hematology Authors: Kaufman, D. S. Tags: INSIDE BLOOD Source Type: research

Circulating CD34+ progenitor cell frequency is associated with clinical and genetic factors
Key Points CD34+ progenitor cell frequency is reduced in older subjects, and is influenced by environmental factors such as smoking and statin use. CD34+ progenitor cell frequency is highly heritable and associated with common genetic variants at several loci.
Source: Blood - February 21, 2013 Category: Hematology Authors: Cohen, K. S., Cheng, S., Larson, M. G., Cupples, L. A., McCabe, E. L., Wang, Y. A., Ngwa, J. S., Martin, R. P., Klein, R. J., Hashmi, B., Ge, Y., O'Donnell, C. J., Vasan, R. S., Shaw, S. Y., Wang, T. J. Tags: Hematopoiesis and Stem Cells, Transplantation, e-Blood Source Type: research

Impact of allogeneic haematopoietic stem cell transplantation in patients with abnl(17p) acute myeloid leukaemia
In conclusion, allogeneic HSCT does not improve survival in patients with abnl(17p) AML as compared to other adverse cytogenetic risk abnormalities.
Source: British Journal of Haematology - February 21, 2013 Category: Hematology Authors: Brigitte Mohr, Johannes Schetelig, Kerstin Schäfer‐Eckart, Norbert Schmitz, Mathias Hänel, Wolf Rösler, Norbert Frickhofen, Hartmut Link, Andreas Neubauer, Ulrich Schuler, Uwe Platzbecker, Jan M. Middeke, Gerhard Ehninger, Martin Bornhäuser, Markus Tags: Research Paper Source Type: research

Treatment of FGF‐2 on stem cells from inflamed dental pulp tissue from human deciduous teeth
ConclusionFGF‐2 expansion of stem cells from inflamed pulp tissues of human deciduous teeth can be a good source of stem cells for future clinical applications and a novel way of using discarded inflamed tissues.© 2013 John Wiley & Sons A/S
Source: Oral Diseases - February 21, 2013 Category: ENT & OMF Authors: Jane C. Kim, Jung‐Chul Park, Sung‐Hwan Kim, Gun‐Il Im, Byung‐Soo Kim, Jongbin Lee, Eun‐Young Choi, Je‐Seon Song, Kyoo‐Sung Cho, Chang‐Sung Kim Tags: Original Article Source Type: research

New therapy for heart failure may enhance body's stem cell response at cardiovascular injury site
(University of South Florida (USF Health)) Cardiovascular disease specialists at Florida Hospital Pepin Heart Institute and the University of South Florida have begun a clinical trial testing a novel gene therapy that may benefit heart failure after ischemic injury. The randomized, placebo-controlled multicenter study, STOP-HF, will enroll 90 patients nationwide.
Source: EurekAlert! - Medicine and Health - February 21, 2013 Category: Global & Universal Source Type: news

Modeling Alzheimer's disease using iPSCs
(Center for iPS Cell Research and Application - Kyoto University) Researchers at Japan's Kyoto University and Nagasaki University have successfully modeled Alzheimer's disease (AD) using both familial and sporadic patient-derived induced pluripotent stem cells, and revealed stress phenotypes and differential drug responsiveness associated with intracellular amyloid beta oligomers in AD neurons and astrocytes.
Source: EurekAlert! - Medicine and Health - February 21, 2013 Category: Global & Universal Source Type: news

'Mesenchymal stem cells improve murine acute coxsackievirus B3-induced myocarditis' [Eur Heart J 2011;32(17):2168-2178, doi:10.1093/eurheartj/ehq467]
Source: European Heart Journal - February 21, 2013 Category: Cardiology Authors: Van Linthout, S., Savvatis, K., Miteva, K., Peng, J., Ringe, J., Warstat, K., Schmidt-Lucke, C., Sittinger, M., Schultheiss, H.- P., Tschope, C. Tags: Corrigendum Source Type: research

Enhanced Osteoblastogenesis of Adipose-Derived Stem Cells on Spermine Delivery via β-Catenin Activation
Stem Cells and Development , Vol. 0, No. 0.
Source: Stem Cells and Development - February 20, 2013 Category: Stem Cells Tags: article Source Type: research

Long-Term Clinical and Molecular Remissions in Patients with Follicular Lymphoma Following High-Dose Therapy and Autologous Stem Cell Transplantation
A German report provided more evidence that long-term complete remissions can be achieved with high-dose chemotherapy and stem cell support in selected patients with follicular lymphoma. Abstract Background:...
Source: OncologySTAT Journal Scans - February 20, 2013 Category: Cancer & Oncology Source Type: research