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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 16.

Tumor necrosis factor–costimulated T lymphocytes from patients with systemic sclerosis trigger collagen production in fibroblasts
ConclusionTNF‐costimulated T lymphocytes from SSc patients have a propensity to secrete profibrotic cytokines, while the ability to produce IL‐10 is weakened. These results suggest that T lymphocytes in SSc support fibrosis, but might lack the capacity to resolve inflammation.
Source: Arthritis and Rheumatism - January 28, 2013 Category: Rheumatology Authors: Thomas Hügle, Steven O'Reilly, Rachel Simpson, Marina D. Kraaij, Venetia Bigley, Matthew Collin, Anja Krippner‐Heidenreich, Jacob M. van Laar Tags: Systemic Sclerosis Source Type: research

The Effect of Mesenchymal Stem Cells on Dynamic Changes of T Cell Subsets in Experimental Autoimmune Uveoretinitis
In this study, we further examined the effects of MSCs on the dynamics of T cell subsets in both eye and spleen and their cytokine production during the course of EAU. We focused on when and where the MSCs had inhibitory effects on Th1 and Th17 cells and how long the inhibitory effect lasted, so as to provide more mechanistic evidence for MSCs on the treatment of uveitis. Compared to the control group, administration of MSCs significantly decreased the production of Th1 and Th17 cytokines, while elevated the production of Th2 and Treg cytokines (IL‐10 and TGF‐b) during the entire course of EAU. Correspondingly, the dyn...
Source: Clinical and Experimental Immunology - January 28, 2013 Category: Allergy & Immunology Authors: Guangda Li, Lifei Yuan, Xinjun Ren, Hong Nian, Lei Zhang, Zhong Chao Han, Xiaorong Li, Xiaomin Zhang Tags: Original Article Source Type: research

An investigation of donor and culture parameters which influence epithelial outgrowths from cultured human cadaveric limbal explants
This study shows that an important factor correlating with growth variation is the duration of corneo‐scleral tissue in organ culture. Interestingly, donor age was not correlated with limbal explant growth. J. Cell. Physiol. © 2012 Wiley Periodicals, Inc.
Source: Journal of Cellular Physiology - January 28, 2013 Category: Cytology Authors: Oliver Baylis, Paul Rooney, Francisco Figueiredo, Majlinda Lako, Sajjad Ahmad Tags: Original Research Article Source Type: research

Mesenchymal stem cells contribute to endogenous FVIII:c production
Abstract Besides the liver, it has been difficult to identify which organ(s) and/or cellular component(s) contribute significantly to the production of human FVIII:c (FVIII). Thus far, only endothelial cells have been shown to constitute a robust extrahepatic source of FVIII, possibly explaining both the diverse presence of FVIII mRNA in the body, and the observed increase in FVIII levels during liver failure. Here, we investigate whether human mesenchymal stem cells (MSC), ubiquitously present in different organs, could also contribute to FVIII production. MSC isolated from human lung, liver, brain, and bone marrow expres...
Source: Journal of Cellular Physiology - January 28, 2013 Category: Cytology Authors: Chad Sanada, Chung‐Jung Kuo, Evan J. Colletti, Melisa Soland, Saloomeh Mokhtari, Mary Ann Knovich, John Owen, Esmail D. Zanjani, Christopher D. Porada, Graça Almeida‐Porada Tags: Original Research Article Source Type: research

Activation and promotion of adipose tissue‐derived mesenchymal stem cells by tumour necrosis factor‐alpha preconditioning for bone tissue engineering
This study investigated the effect of short‐term (three days) preconditioning with tumour necrosis factor‐alpha (TNF‐α) on proliferation, mobilization and differentiation of adipose tissue‐derived mesenchymal stem cells (ASCs). We demonstrated that TNF‐α pre‐conditioning increased proliferation, mobilization, and osteogenic differentiation of ASCs and up‐regulated bone morphogenetic protein‐2 (BMP‐2) protein levels. BMP‐2 silencing by siRNA partially inhibited osteogenic differentiation of ASCs induced by TNF‐α; BMP‐2 pre‐conditioning also significantly increased osteogenic differentiation of A...
Source: Journal of Cellular Physiology - January 28, 2013 Category: Cytology Authors: ZuFu Lu, GuoCheng Wang, Colin R Dunstan, YongJun Chen, William Yenn‐Ru Lu, Ben Davies, Hala Zreiqat Tags: Original Research Article Source Type: research

Human NG2+ adipose stem cells loaded in vivo on a new crosslinked hyaluronic acid‐lys scaffold fabricate a skeletal muscle tissue
In conclusion, our study provides novel evidence that NG2+ ASCs loaded on XHA scaffolds are able to fabricate a human skeletal muscle tissue in vivo without the need of a myogenic pre‐differentiation step in vitro. We emphasize the translational significance of our findings for human skeletal muscle regeneration. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.
Source: Journal of Cellular Physiology - January 28, 2013 Category: Cytology Authors: Vincenzo Desiderio, Francesco De Francesco, Chiara Schiraldi, Alfredo De Rosa, Annalisa La Gatta, Francesca Paino, Riccardo d'Aquino, Giuseppe Andrea Ferraro, Virginia Tirino, Gianpaolo Papaccio Tags: Original Research Article Source Type: research

Stem cells useful for stroke recovery
Brain and nerve repair witnessed in animal tests Related items from OnMedicaStroke therapists must offer seven-day workingParacetamol could improve outcome after strokeStatin use cuts stroke risk by a fifthAlso in the press
Source: OnMedica Latest News - January 28, 2013 Category: UK Health Source Type: news

Stem cell 'first aid' for rat stroke
Stem cells given in the vital period immediately after a stroke may aid recovery, suggest researchers.
Source: BBC News | Health | UK Edition - January 27, 2013 Category: Consumer Health News Source Type: news

Research using stem cells shows promise for stroke patients
Stem cells given to a patient directly after they have had a stroke may significantly aid their recovery, Bolivian researchers have discovered.
Source: Pharmaceutical Technology - January 27, 2013 Category: Pharmaceuticals Source Type: news

Stem Cell Treatment for Chronic Lung Diseases
Source: Respiration - January 27, 2013 Category: Respiratory Medicine Source Type: research

Patients' own skin cells are transformed into heart cells to create 'disease in a dish'
Researchers have unveiled the first maturation-based "disease in a dish" model for arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C). The model was created using induced pluripotent stem cells and a new method to mimic metabolic maturity. This model is likely more relevant to human ARVD/C than other models and therefore better suited for studying the disease and testing new treatments.
Source: ScienceDaily Headlines - January 27, 2013 Category: Science Source Type: news

Generation of mesenchymal stem cells as a medicinal product in organ transplantation.
Abstract PURPOSE OF REVIEW: Mesenchymal stem cells (MSCs) are emerging as an alternative treatment in solid-organ transplantation. The use of MSCs as a therapeutic product requires the translation of basic research protocols into a production process under good manufacturing practice (GMP) to obtain a safe product of high quality. This requires a different mindset from the academic setting of changing protocols into a well defined, controlled and documented process. This review describes some of the challenges faced by culturing MSCs as a medicinal product. RECENT FINDINGS: Clinical-grade MSCs are used in the c...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Verbeek R Tags: Curr Opin Organ Transplant Source Type: research

Mesenchymal stem cells in organ and tissue transplantation: one for all seasons?
Mesenchymal stem cells in organ and tissue transplantation: one for all seasons? Curr Opin Organ Transplant. 2013 Feb;18(1):22-3 Authors: Eggenhofer E PMID: 23222175 [PubMed - in process]
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Eggenhofer E Tags: Curr Opin Organ Transplant Source Type: research

Machine perfusion in organ transplantation: a tool for ex-vivo graft conditioning with mesenchymal stem cells?
This article aims to review the current knowledge on machine perfusion of the various solid thoracic and abdominal organs, and to discuss the new possibility of conditioning and treating grafts with mesenchymal stem cells (MSCs) during machine perfusion. RECENT FINDINGS: Different methods of machine perfusion have been described varying among organs in temperature and composition of perfusate. Commercial devices have recently become available for machine perfusion of all organs, with the largest clinical experience acquired in kidney and lung transplantation. Clinical studies are ongoing for liver, heart, and pancreas....
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Van Raemdonck D, Neyrinck A, Rega F, Devos T, Pirenne J Tags: Curr Opin Organ Transplant Source Type: research

Multiple Myeloma Patients May Benefit From New Research
A study led by Robert G. Hawley, Ph.D., professor and chair of the department of anatomy and regenerative biology at the George Washington University (GW) School of Medicine and Health Sciences (SMHS), may help predict which patients with multiple myeloma will respond better to certain treatments. The study, titled "Identification of an ABCB1 (P-glycoprotein)-positive carfilzomib-resistant myeloma subpopulation by the pluripotent stem cell fluorescent dye CDy1," was published in the American Journal of Hematology and is available online...
Source: Health News from Medical News Today - January 27, 2013 Category: Consumer Health News Tags: Lymphoma / Leukemia / Myeloma Source Type: news

Hematopoetic and mesenchymal stem cell transplantation in the treatment of refractory systemic lupus erythematosus - Where are we now?
Abstract Cell based therapies are of increasing interest in the treatment of systemic lupus due to their potential for long term suppression or cure of disease. Two methods for stem cell transplantation are currently being investigated/performed for treatment of lupus. Autologous hematopoetic stem cell transplantation is used in patients refractory to standard therapy. The morbidity and mortality of the procedure limit its use to select patients. Results indicate 50% long term disease free survival. The technical difficulty of the procedure requires it to be performed only in experienced centers. Mesenchymal stem c...
Source: Clinical Immunology - January 27, 2013 Category: Allergy & Immunology Authors: Collins E, Gilkeson G Tags: Clin Immunol Source Type: research

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
rkus Grompe & Hans Clevers The Wnt target gene Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon, stomach and hair follicles. A three-dimensional culture system allows long-term clonal expansion of single Lgr5+ stem cells into transplantable organoids (budding cysts) that retain many characteristics of the original epithelial architecture. A crucial component of the culture medium is the Wnt agonist RSPO1, the recently discovered ligand of LGR5. Here we show that Lgr5-lacZ is not expressed in ...
Source: Nature - January 27, 2013 Category: Research Authors: Meritxell HuchCraig DorrellSylvia F. BojJohan H. van EsVivian S. W. LiMarc van de WeteringToshiro SatoKarien HamerNobuo SasakiMilton J. FinegoldAnnelise HaftRobert G. VriesMarkus GrompeHans Clevers Tags: Letter Source Type: research

Studying arrhythmogenic right ventricular dysplasia with patient-specific iPSCs
ge & Huei-Sheng Vincent Chen Cellular reprogramming of somatic cells to patient-specific induced pluripotent stem cells (iPSCs) enables in vitro modelling of human genetic disorders for pathogenic investigations and therapeutic screens. However, using iPSC-derived cardiomyocytes (iPSC-CMs) to model an adult-onset heart disease remains challenging owing to the uncertainty regarding the ability of relatively immature iPSC-CMs to fully recapitulate adult disease phenotypes. Arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C) is an inherited heart disease characterized by pathological fatty infiltration...
Source: Nature - January 27, 2013 Category: Research Authors: Changsung KimJohnson WongJianyan WenShirong WangCheng WangSean SpieringNatalia G. KanSonia ForcalesPier Lorenzo PuriTeresa C. LeoneJoseph E. MarineHugh CalkinsDaniel P. KellyDaniel P. JudgeHuei-Sheng Vincent Chen Tags: Letter Source Type: research

The Anti-Fibrotic Peptide Ac-Sdkp: Opportunities For Ace Inhibitor Design.
Abstract The renin-angiotensin system (RAS) is central to regulation of blood pressure and electrolyte homeostasis. Angiotensin-converting enzyme (ACE), a key protease in the RAS, has a range of substrates, including N-acetyl-Ser-Asp-Lys-Pro (Ac-SDKP). Ac-SDKP is cleared almost exclusively by ACE and specifically by the N-domain active site of this enzyme. Ac-SDKP is a negative regulator of haematopoietic stem cell differentiation and is a potent anti-fibrotic agent. In this review, the physiological actions of Ac-SDKP will be presented together with the potential clinical utility of raising Ac-SDKP levels. This em...
Source: Clinical and Experimental Pharmacology and Physiology - January 27, 2013 Category: Drugs & Pharmacology Authors: Douglas RG, Ehlers MR, Sturrock ED Tags: Clin Exp Pharmacol Physiol Source Type: research

TAF4b and TAF4 differentially regulate mouse embryonic stem cells maintenance and proliferation
TAF4b is a cell type‐specific subunit of the general transcription factor TFIID. Here, we show that TAF4b is highly expressed in embryonic stem cells (ESC) and is down‐regulated upon differentiation. To examine the role of TAF4b in ESC, we applied a knockdown (KD) approach. TAF4b depletion is associated with morphological changes and reduced expression of the self‐renewal marker alkaline phosphatase. In contrast, KD of TAF4, a ubiquitously expressed TAF4b paralog, retained and even stabilized ESC stemness. Retinoic acid‐induced differentiation was facilitated in the absence of TAF4b but was significantly delayed by...
Source: Genes to Cells - January 27, 2013 Category: Genetics & Stem Cells Authors: Anat Bahat, Ranit Kedmi, Kfir Gazit, Inna Richardo‐Lax, Elena Ainbinder, Rivka Dikstein Tags: Original Article Source Type: research

Studying arrhythmogenic right ventricular dysplasia with patient-specific iPSCs
Nature advance online publication 27 January 2013. doi:10.1038/nature11799 Authors: Changsung Kim, Johnson Wong, Jianyan Wen, Shirong Wang, Cheng Wang, Sean Spiering, Natalia G. Kan, Sonia Forcales, Pier Lorenzo Puri, Teresa C. Leone, Joseph E. Marine, Hugh Calkins, Daniel P. Kelly, Daniel P. Judge & Huei-Sheng Vincent Chen Cellular reprogramming of somatic cells to patient-specific induced pluripotent stem cells (iPSCs) enables in vitro modelling of human genetic disorders for pathogenic investigations and therapeutic screens. However, using iPSC-derived cardiomyocytes (iPSC-CMs) to model an adult-onset heart disease...
Source: Nature AOP - January 27, 2013 Category: Research Authors: Changsung KimJohnson WongJianyan WenShirong WangCheng WangSean SpieringNatalia G. KanSonia ForcalesPier Lorenzo PuriTeresa C. LeoneJoseph E. MarineHugh CalkinsDaniel P. KellyDaniel P. JudgeHuei-Sheng Vincent Chen Tags: Letter Source Type: research

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
Nature advance online publication 27 January 2013. doi:10.1038/nature11826 Authors: Meritxell Huch, Craig Dorrell, Sylvia F. Boj, Johan H. van Es, Vivian S. W. Li, Marc van de Wetering, Toshiro Sato, Karien Hamer, Nobuo Sasaki, Milton J. Finegold, Annelise Haft, Robert G. Vries, Markus Grompe & Hans Clevers The Wnt target gene Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon, stomach and hair follicles. A three-dimensional culture system allows long-term clonal expansion of single Lgr5+ stem cell...
Source: Nature AOP - January 27, 2013 Category: Research Authors: Meritxell HuchCraig DorrellSylvia F. BojJohan H. van EsVivian S. W. LiMarc van de WeteringToshiro SatoKarien HamerNobuo SasakiMilton J. FinegoldAnnelise HaftRobert G. VriesMarkus GrompeHans Clevers Tags: Letter Source Type: research

Prognostic impact of immune status and hematopoietic recovery before and after fludarabine, IV busulfan, and antithymocyte globulins (FB2 regimen) reduced‐intensity conditioning regimen (RIC) allogeneic stem cell transplantation (allo‐SCT)
Abstract This retrospective analysis aimed to assess hematopoietic and immune recovery in a cohort of 53 patients [males: n = 33; median age: 59 yr (range: 22–70)] who received a FB2 (fludarabine 120–150 mg/m² + IV busulfan 6.4 mg/kg + antithymocyte globulin thymoglobulin 5 mg/kg) reduced‐intensity conditioning (RIC) allo‐stem cells transplantations (SCT). With a median follow‐up of 19 months (range: 2–53), the 2‐yr overall survival, disease‐free survival (DFS), relapse incidence, and non‐relapse mortality were 63%, 59.5%, 35%, and 6%, respectively. In univariate analysis, the factors correlat...
Source: European Journal of Haematology - January 27, 2013 Category: Hematology Authors: Amandine Bourgeois, Elsa Lestang, Thierry Guillaume, Jacques Delaunay, Sameh Ayari, Nicolas Blin, Aline Clavert, Benoit Tessoulin, Viviane Dubruille, Beatrice Mahe, Virginie Roland, Thomas Gastinne, Steven Gouill, Philippe Moreau, Mohamad Mohty, Lucie Pla Tags: Original Article Source Type: research

Stem cells aid recovery from stroke
(BioMed Central) Stem cells from bone marrow or fat improve recovery after stroke in rats, finds a study published in BioMed Central's open access journal Stem Cell Research & Therapy. Treatment with stem cells improved the amount of brain and nerve repair and the ability of the animals to complete behavioral tasks.
Source: EurekAlert! - Medicine and Health - January 27, 2013 Category: Global & Universal Source Type: news

Patients' own skin cells are transformed into heart cells to create 'disease in a dish'
(Sanford-Burnham Medical Research Institute) In a paper published Jan. 27 in Nature, researchers at Sanford-Burnham Medical Research Institute and Johns Hopkins University unveil the first maturation-based "disease in a dish" model for arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C). The model was created using induced pluripotent stem cells and a new method to mimic metabolic maturity. This model is likely more relevant to human ARVD/C than other models and therefore better suited for studying the disease and testing new treatments.
Source: EurekAlert! - Medicine and Health - January 27, 2013 Category: Global & Universal Source Type: news

Nanoscale Film Fabrication of Various Peptides on Neural Stem Cell Chip
Source: Journal of Biomedical Nanotechnology - January 26, 2013 Category: Nanotechnology Authors: Kim, Tae-HyungKo, Eun-BiKim, Soon-JoongChoi, Jeong-Woo Tags: Research Articles Source Type: research

B4 androgen ablation: attacking the prostate cancer stem cell
There is increasing evidence that prostate cancers in rodent models and in men contain a cellular subpopulation that displays stem cell properties. These prostate cancer stem cells (PCSCs) lack androgen receptor expression and are increased in castration-resistant disease. In this issue of the JCI, a study from Yoshioka et al. demonstrates that PCSCs are regulated by a pathway in which α6β4 integrin amplifies signaling through ErbB2 and c-Met receptors. Targeting this pathway provides a novel therapeutic strategy for hormone refractory prostate cancer.
Source: Journal of Clinical Investigation - January 26, 2013 Category: Biomedical Science Authors: Max S. Wicha Source Type: research

β4 Integrin signaling induces expansion of prostate tumor progenitors
The contextual signals that regulate the expansion of prostate tumor progenitor cells are poorly defined. We found that a significant fraction of advanced human prostate cancers and castration-resistant metastases express high levels of the β4 integrin, which binds to laminin-5. Targeted deletion of the signaling domain of β4 inhibited prostate tumor growth and progression in response to loss of p53 and Rb function in a mouse model of prostate cancer (PB-TAg mice). Additionally, it suppressed Pten loss-driven prostate tumorigenesis in tissue recombination experiments. We traced this defect back to an inability ...
Source: Journal of Clinical Investigation - January 26, 2013 Category: Biomedical Science Authors: Toshiaki Yoshioka, Javier Otero, Yu Chen, Young-Mi Kim, Jason A. Koutcher, Jaya Satagopan, Victor Reuter, Brett Carver, Elisa de Stanchina, Katsuhiko Enomoto, Norman M. Greenberg, Peter T. Scardino, Howard I. Scher, Charles L. Sawyers, Filippo G. Giancott Source Type: research

Lucy Mangan: wanted – mother for Neanderthal baby
'Don't you long, occasionally, for something really, really interesting, something different, something overwhelmingly "other" to happen?'It's possible that the snow has driven me stir crazy. Although, as someone who, when under stress, still draws diagrams of the underground, Womble burrow-based home that she plans to build when she wins the lottery, I think that is highly unlikely. In any case, I have been seized by the idea of having another baby.Not just any old baby – I've already got one of those – but a Neanderthal baby. Earlier this week Professor George Church, a genetic researcher at Harva...
Source: Guardian Unlimited Science - January 26, 2013 Category: Science Authors: Lucy Mangan Tags: The Guardian Family Genetics Evolution Biology Parents and parenting Anthropology Features Life and style Neanderthal man Science Source Type: news

High-dose chemotherapy and autologous stem-cell transplantation in Korean patients with relapsed or refractory Hodgkin lymphoma.
Abstract High-dose chemotherapy and autologous stem-cell transplantation (HDCT-ASCT) is a standard therapy for patients with relapsed or refractory Hodgkin lymphoma (HL). However, its efficacy in Asian patients has not been well investigated. A retrospective analysis of outcomes in 10 consecutive patients who underwent ASCT for HL in a single Korean centre from August 2005 to September 2010 was conducted. The median age was 34.5 years (range 17-64 years) and seven patients were male. Six patients were of stage III-IV at presentation. B symptoms were present in six patients. International Prognostic Score (IPS) wa...
Source: International Journal of Hematology - January 26, 2013 Category: Hematology Authors: Park K, Yoon DH, Kim S, Park CS, Huh J, Lee SW, Suh C Tags: Int J Hematol Source Type: research

Bioinformatics Tools Allow Targeted Selection of Chromosome Enumeration Probes and Aneuploidy Detection
Accurate determination of cellular chromosome complements is a highly relevant issue beyond prenatal/pre-implantation genetic analyses or stem cell research, because aneusomy may be an important mechanism by which organisms control the rate of fetal cellular proliferation and the fate of regenerating tissues. Typically, small amounts of individual cells or nuclei are assayed by in situ hybridization using chromosome-specific DNA probes. Careful probe selection is fundamental to successful hybridization experiments. Numerous DNA probes for chromosome enumeration studies are commercially available, but their use in multiplex...
Source: Journal of Histochemistry and Cytochemistry - January 26, 2013 Category: Biochemistry Authors: O'Brien, B., Zeng, H., Polyzos, A. A., Lemke, K. H., Weier, J. F., Wang, M., Zitzelsberger, H. F., Weier, H.-U. G. Tags: Articles Source Type: research

Cell and Tissue Microarray Technologies for Protein and Nucleic Acid Expression Profiling
Tissue microarray (TMA) and cell microarray (CMA) are two powerful techniques that allow for the immunophenotypical characterization of hundreds of samples simultaneously. In particular, the CMA approach is particularly useful for immunophenotyping new stem cell lines (e.g., cardiac, neural, mesenchymal) using conventional markers, as well as for testing the specificity and the efficacy of newly developed antibodies. We propose the use of a tissue arrayer not only to perform protein expression profiling by immunohistochemistry but also to carry out molecular genetics studies. In fact, starting with several tissues or cell ...
Source: Journal of Histochemistry and Cytochemistry - January 26, 2013 Category: Biochemistry Authors: Cardano, M., Diaferia, G. R., Falavigna, M., Spinelli, C. C., Sessa, F., DeBlasio, P., Biunno, I. Tags: Articles Source Type: research

Neural stem cell-like cells derived from autologous bone mesenchymal stem cells for the treatment of patients with cerebral palsy
Conclusion: Our results indicated that NSC-like cells are safe and effective for the treatment of motor deficits related to cerebral palsy. Further randomised clinical trials are necessary to establish the efficacy of this procedure.
Source: Journal of Translational Medicine - January 26, 2013 Category: Research Authors: Guojun ChenYali WangZhenyu XuFeng FangRenmei XuYue WangXiaoli HuLixing FanHouqi Liu Source Type: research

Protection of genetic heritage in the era of cloning
Research on human beings has expanded greatly due to progress and the evolution of society as well as customs. Not only the unceasing development of research on human beings, but also interference in the beginning and end of life with homologous and heterogonous human reproduction, surrogate motherhood, cloning, gene therapies, eugenics,euthanasia, dysthanasia, orthothanasia, assisted suicide, genetic engineering, reassignment surgery in cases of transsexuality, the use of recombinant DNA technology and embryonic stem cells, transplantation of human organs and tissues, biotechnology and many other scientific advances. Scie...
Source: Revista Brasileira de Hematologia e Hemoterapia - January 25, 2013 Category: Hematology Source Type: research

Early lymphocyte recovery as a predictor of outcome, including relapse, after hematopoieticstem cell transplantation
CONCLUSION: The evaluation of lymphocyte recovery on Day +30 is not a good predictor of relapse after transplant however patients with inadequate lymphocyte recovery had worse overall survival and relapse-free survival. Inadequate lymphocyte recovery on Day +100 is correlated with higher cumulative relapse as well as lower overall survival and relapse-free survival.
Source: Revista Brasileira de Hematologia e Hemoterapia - January 25, 2013 Category: Hematology Source Type: research

Periodontal health influences stem cell transplant success
Periodontal infection can increase the risk for developing bacteremia during the neutropenic phase of allogeneic hematopoietic stem cell transplantation, research suggests.
Source: MedWire News - Infectious Diseases - January 25, 2013 Category: Infectious Diseases Source Type: news

Cardiac Development Needs More Than Protein-Coding Genes
Biologists find that long non-coding RNA molecules are necessary to regulate differentiation of embryonic stem cells into cardiac cells. When the human genome was sequenced, biologists were surprised to find that very little of the genome - less than 3 percent - corresponds to protein-coding genes. What, they wondered, was all the rest of that DNA doing? It turns out that much of it codes for genetic snippets known as long non-coding RNAs, or lncRNAs. In recent years, scientists have found that these molecules often help to regulate which genes get turned on or off inside a cell...
Source: Health News from Medical News Today - January 25, 2013 Category: Consumer Health News Tags: Cardiovascular / Cardiology Source Type: news

Stem Cell Therapies May Improve Following Finding That Retrovirus In The Human Genome Is Active In Pluripotent Stem Cells
A retrovirus called HERV-H, which inserted itself into the human genome millions of years ago, may play an important role in pluripotent stem cells, according to a new study published in the journal Retrovirology by scientists at UMass Medical School. Pluripotent stem cells are capable of generating all tissue types, including blood cells, brain cells and heart cells...
Source: Health News from Medical News Today - January 25, 2013 Category: Consumer Health News Tags: Stem Cell Research Source Type: news

New Technology For Delivering RNA, Proteins And Nanoparticles By Putting The Squeeze On Cells
Living cells are surrounded by a membrane that tightly regulates what gets in and out of the cell. This barrier is necessary for cells to control their internal environment, but it makes it more difficult for scientists to deliver large molecules such as nanoparticles for imaging, or proteins that can reprogram them into pluripotent stem cells. Researchers from MIT have now found a safe and efficient way to get large molecules through the cell membrane, by squeezing the cells through a narrow constriction that opens up tiny, temporary holes in the membrane...
Source: Health News from Medical News Today - January 25, 2013 Category: Consumer Health News Tags: Stem Cell Research Source Type: news

All's well that ends well: alternative polyadenylation and its implications for stem cell biology.
Abstract Stem cell quiescence, activation, and differentiation are governed by a complex network of molecular pathways. There has been a growing recognition that posttranscriptional modifications, such as alternative polyadenylation (APA) of transcripts, play an important role in regulating gene expression and function. Recent analyses of stem cell populations have suggested that APA controls stem cell fate and behavior. Here, we review recent developments that have shaped our understanding of the control of stem cell behavior by APA and we highlight promising areas for future investigation. PMID: 23357469 [Pub...
Source: Current Opinion in Cell Biology - January 25, 2013 Category: Cytology Authors: Mueller AA, Cheung TH, Rando TA Tags: Curr Opin Cell Biol Source Type: research

Prognostic value of circulating levels of stem cell growth factor beta (SCGF beta) in patients with Chagas' disease and idiopathic dilated cardiomyopathy.
Abstract Chagas' disease (CD) often leads to dilated cardiomyopathy (DCM), and during its chronic stage hematopoietic stem or progenitor cells are involved in its pathological process. However, it is not clear whether stem cell growth factor (SCGF) beta can be regulated in patients with CD and idiopathic DCM. In present study, we aim to investigate the plasma SCGF beta concentration and its correlation with echocardiographic parameters and clinical outcome. In this prospective cohort study, SCGF beta levels were quantified in patients with CD (n=94), DCM (n=48), and control healthy subjects (n=25). In comparison wi...
Source: Cytokine - January 25, 2013 Category: Molecular Biology Authors: Wang Y, Khan A, Heringer-Walther S, Schultheiss HP, Moreira MD, Walther T Tags: Cytokine Source Type: research

Collagen/ß1 integrin interaction is required for embryoid body formation during cardiogenesis from murine induced pluripotent stem cells
Conclusions: These findings indicate that collagen/β1 integrin interaction is required for the growth and cardiac differentiation of miPSC-derived EBs and will be helpful in future engineering of the matrix microenvironment within EBs to efficiently direct the cardiac fate of pluripotent stem cells to promote cardiovascular regeneration.
Source: BMC Cell Biology - Latest articles - January 25, 2013 Category: Cytology Authors: Di ZengDong-Bo OuTing WeiLu DingXiong-Tao LiuXin-Lin HuXue LiQiang-Sun Zheng Source Type: research

Depletion of JARID1B induces cellular senescence in human colorectal cancer.
In this study, we investigated the functional roles of the H3K4 demethylase, jumonji, AT rich interactive domain 1B (JARID1B), an epigenetic factor required for the continuous cell growth of melanomas, in CRC. We found that CD44+/aldehyde dehydrogenase (ALDH)+ slowly proliferating immature CRC stem cell populations expressed relatively low levels of JARID1B and the differentiation marker, CD20, as well as relatively high levels of the tumor suppressor, p16̸INK4A. Of note, lentiviral‑mediated continuous JARID1B depletion resulted in the loss of epithelial differentiation and suppressed CRC cell growth, which was associa...
Source: International Journal of Oncology - January 25, 2013 Category: Cancer & Oncology Authors: Ohta K, Haraguchi N, Kano Y, Kagawa Y, Konno M, Nishikawa S, Hamabe A, Hasegawa S, Ogawa H, Fukusumi T, Uemura M, Nishimura J, Hata T, Takemasa I, Mizushima T, Noguchi Y, Ozaki M, Kudo T, Sakai D, Satoh T, Fukami M, Ishii M, Yamamoto H, Doki Y, Mori M, Is Tags: Int J Oncol Source Type: research

Phase II Study of Bendamustine in Relapsed and Refractory Hodgkin Lymphoma [Hematologic Malignancies]
Conclusion This study confirms the efficacy of bendamustine in heavily pretreated patients with HL. These results support current and future studies evaluating bendamustine combinations in relapsed and refractory HL.
Source: Journal of Clinical Oncology - January 25, 2013 Category: Cancer & Oncology Authors: Moskowitz, Hamlin, Perales, Gerecitano, Horwitz, Matasar, Noy, Palomba, Portlock, Straus, Graustein, Zelenetz, Moskowitz Tags: Chemotherapy Hematologic Malignancies Source Type: research

Collagen/beta1 integrin interaction is required for embryoid body formation during cardiogenesis from murine induced pluripotent stem cells
Conclusions: These findings indicate that collagen/beta1 integrin interaction is required for the growth and cardiac differentiation of miPSC-derived EBs and will be helpful in future engineering of the matrix microenvironment within EBs to efficiently direct the cardiac fate of pluripotent stem cells to promote cardiovascular regeneration.
Source: BMC Cell Biology - Latest articles - January 25, 2013 Category: Cytology Authors: Di ZengDong-Bo OuTing WeiLu DingXiong-Tao LiuXin-Lin HuXue LiQiang-Sun Zheng Source Type: research

Matrix-bound heparan sulfate is essential for the growth and pluripotency of human embryonic stem cells
Human embryonic stem (hES) cell production of heparan sulfate influences cell fate and pluripotency. Human ES cells remain pluripotent in vitro through the action of growth factors signaling, and the activity of these factors depends on interaction with specific receptors and also with heparan sulfate. Here, we tested the hypothesis that matrix-associated heparan sulfate is enough to maintain hES cells under low fibroblast growth factor-2 concentration in the absence of live feeder cells. To pursue this goal, we compared hES cells cultured either on coated plates containing live murine embryonic fibroblasts (MEFs) or on a ...
Source: Glycobiology - January 25, 2013 Category: Biology Authors: Stelling, M. P., Lages, Y. M., Tovar, A. M., Mourao, P. A., Rehen, S. K. Tags: ORIGINAL ARTICLES Source Type: research

A novel antibody for human induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures
We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cell lysates gave a single but an unusually diffuse band at a position corresponding to >250 kDa. The antigen protein was isolated from the induced pluripotent stem (iPS) cell lysates with an affinity column of R-10G. The R-10G positive band was resistant to digestion with peptide N-glycanase F (PNGase F), neu...
Source: Glycobiology - January 25, 2013 Category: Biology Authors: Kawabe, K., Tateyama, D., Toyoda, H., Kawasaki, N., Hashii, N., Nakao, H., Matsumoto, S., Nonaka, M., Matsumura, H., Hirose, Y., Morita, A., Katayama, M., Sakuma, M., Kawasaki, N., Furue, M. K., Kawasaki, T. Tags: ORIGINAL ARTICLES Source Type: research

Regulation of redox signalling by an electrophilic cyclic nucleotide
Reactive oxygen species (ROS) have been believed to be toxic substances that induce nonspecific damage in various biological molecules. ROS toxicology is now developing an emerging concept for physiological functions of ROS in the regulation of cell signal transductions. ROS signalling functions and their mechanisms are precisely regulated by several endogenous moderate electrophiles that are themselves generated from ROS during diverse physiological and pathophysiological cellular responses. The chemical biology of electrophiles is an emerging scientific area involving molecular mechanisms that conduct ROS cell signals th...
Source: Journal of Biochemistry - January 25, 2013 Category: Biochemistry Authors: Akaike, T., Nishida, M., Fujii, S. Tags: JB Reviews Source Type: research

ERK5 Mediates Prolactin-induced Adult Neurogenesis [Neurobiology]
We report here for the first time that prolactin activates extracellular signal-regulated kinase 5 (ERK5), a MAP kinase that is specifically expressed in the neurogenic regions of the adult mouse brain. Knockdown of ERK5 by retroviral infection of shRNA attenuates prolactin-stimulated neurogenesis in SVZ-derived adult neural stem/progenitor cells (aNPCs). Inducible erk5 deletion in adult neural stem cells of transgenic mice inhibits neurogenesis in the SVZ and OB following prolactin infusion or mating/pregnancy. These results identify ERK5 as a novel and critical signaling mechanism underlying prolactin-induced adult neurogenesis.
Source: Journal of Biological Chemistry - January 25, 2013 Category: Chemistry Authors: Wang, W., Pan, Y.-W., Wietecha, T., Zou, J., Abel, G. M., Kuo, C. T., Xia, Z. Tags: Signal Transduction Source Type: research