Urology & Nephrology Research This is an OPML file. It can be used to export all the MedWorm RSS feeds on this topic into your personal RSS reader (usually you have to save this file to your own computer before clicking on an Import OPML command in your own feed reader to upload the file which will then import all the feeds) or it can be used by webmasters to integrate MedWorm feeds with their own website. This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 19.

Timing of hepatectomy in resectable synchronous colorectal liver metastases (SCRLM): Simultaneous or delayed?
Conclusions:Simultaneous resection is as efficient as delayed procedure for the long‐term survival. There are evidences implied that in SCRLM patients simultaneous resection is an acceptable and safe option with the carefully good selected conditions.Due to inherent limitations of the present study, future randomized controlled trials are eagerly awaited to confirm this conclusion. (HEPATOLOGY 2013.)
Source: Hepatology - January 28, 2013 Category: Internal Medicine Authors: Zi Yin, Chao Liu, Yajin Chen, Yu Bai, hangzhen Shang, Rongyan Yin, Dong Yin, Jie Jie Wang Tags: Clinical Observations in Hepatology Source Type: research

MDM2 SNP309 promoter polymorphism and p53 mutations in urinary bladder carcinoma stage T1
Conclusions: MDM2 SNP309 promoter polymorphism and mutations in p53 were not associated with worse prognosis in this cohort of patients with primary stage T1 urinary bladder carcinoma. However, patients with abnormal p16 expression and a mutated p53 gene had a higher rate of and a shorter time to progression, and p53 gene mutation was associated with an abnormal immunohistochemistry for p53 at a cut-off of 50%.
Source: BMC Urology - Latest articles - January 28, 2013 Category: Urology & Nephrology Authors: Hans OlssonPer HultmanJohan RosellPeter SöderkvistStaffan Jahnson Source Type: research

Effect of folic acid and zinc sulphate on endocrine parameters and seminal antioxidant level after varicocelectomy
This study aimed to evaluate serum hormonal level (inhibin B, FSH and testosterone) and seminal plasma antioxidant defence levels after folic acid and zinc sulphate administration in varicocelectomised patients. Participants were randomly allocated to four experimental groups. Our randomisation schedule was as follows: zinc sulphate/folic acid, folic acid, zinc sulphate and placebo. The patients underwent varicocelectomy, before which a blood and semen sample were obtained and also three and six months after varicocelectomy for evaluation of blood hormonal level (FSH, testosterone, inhibin B) and seminal oxidative stress s...
Source: Andrologia - January 28, 2013 Category: Urology & Nephrology Authors: S. N. Nematollahi‐Mahani, G. H. Azizollahi, M. R. Baneshi, Z. Safari, S. Azizollahi Tags: Original Article Source Type: research

Assessment of sperm hyperactivated motility and acrosome reaction can discriminate the use of spermatozoa for conventional in vitro fertilisation or intracytoplasmic sperm injection: Preliminary results
Summary Basic semen analysis is insufficient for determining the fertility potential. The aim of this study was to determine if hyperactivated motility (HAM) and acrosome reaction (AR) can be useful tests for evaluating semen quality during male infertility evaluations and to help the clinician decide whether regular insemination or intracytoplasmic sperm injection (ICSI) is preferable during in vitro fertilisation. A prospective study was conducted. Patients with normal sperm according to World Health Organization guidelines who underwent IVF treatment and planned regular insemination were asked to participate. A portion...
Source: Andrologia - January 28, 2013 Category: Urology & Nephrology Authors: A. Wiser, S. Sachar, Y. Ghetler, A. Shulman, H. Breitbart Tags: Short Communication Source Type: research

Multipeak fat‐corrected complex R2* relaxometry: Theory, optimization, and clinical validation
Conclusion:The use of complex fitting and fat‐correction improves the robustness, noise performance, and accuracy of R2* measurements, and are necessary to establish R2* as quantitative imaging biomarker in the liver. Magn Reson Med, 2013. © 2013 Wiley Periodicals, Inc.
Source: Magnetic Resonance in Medicine - January 28, 2013 Category: Radiology Authors: Diego Hernando, J. Harald Kramer, Scott B. Reeder Tags: Full Paper Source Type: research

Improved MRI R2* relaxometry of iron‐loaded liver with noise correction
In this study, two noise‐corrected models were proposed for the liver R2* quantification: fitting the signal to the first moment and fitting the squared signal to the second moment in the presence of the noncentral chi noise. These two models were compared with the widely implemented offset and truncation models on both simulation and in vivo data. The results demonstrated that the “slow decay component” of the liver R2* was mainly caused by the noise. The offset model considerably overestimated R2* values by incorrectly adding a constant to account for the slow decay component. The truncation model generally produce...
Source: Magnetic Resonance in Medicine - January 28, 2013 Category: Radiology Authors: Yanqiu Feng, Taigang He, Peter D. Gatehouse, Xinzhong Li, Mohammed Harith Alam, Dudley J. Pennell, Wufan Chen, David N. Firmin Tags: Full Paper Source Type: research

First case of spontaneous myospherulosis of the liver
Introduction Myospherulosis is a rare interaction of extravasated erythrocytes and lipids, resulting in the degeneration of erythrocytes, which are subsequently enclosed by a lipid membrane. Thus, histologically typical, saccular parent bodies containing variable numbers of approximately 6 µm spherules are formed.1 The lesions are usually small (few millimetres to few centimeters) and do not cause any symptoms except swelling at the involved site. Differential diagnoses include carcinomas, metastases, osteofibrosis or echinococcosis due to alikeness in radiographic imaging and morphology. In addition, myospherul...
Source: Journal of Clinical Pathology - January 28, 2013 Category: Pathology Authors: Phillip, V., Becker, K., Gaa, J., Kleeff, J., Schmid, R. M., Bajbouj, M. Tags: PostScript Source Type: research

Prostate needle biopsy processing: a survey of laboratory practice across Europe
Conclusions There are marked differences in procurement, handling and processing of PNBx in laboratories across Europe. This data can help the development of best practice guidelines.
Source: Journal of Clinical Pathology - January 28, 2013 Category: Pathology Authors: Varma, M., Berney, D. M., Algaba, F., Camparo, P., Comperat, E., Griffiths, D. F. R., Kristiansen, G., Lopez-Beltran, A., Montironi, R., Egevad, L. Tags: Clinical diagnostic tests Original articles Source Type: research

Her2 amplification distinguishes a subset of non-muscle-invasive bladder cancers with a high risk of progression
Conclusions A subset of high-grade NMIBCs contain Her2 amplification and are associated with markedly aggressive behaviour. Her2 diagnostics are valuable for distinguishing patients who require diligent surveillance and would potentially benefit from anti-Her2 therapies.
Source: Journal of Clinical Pathology - January 28, 2013 Category: Pathology Authors: Chen, P. C.-H., Yu, H.-J., Chang, Y.-H., Pan, C.-C. Tags: Molecular genetics, Immunology (including allergy), Urological cancer Original articles Source Type: research

Participation of natural killer cells in the pathogenesis of bile duct lesions in biliary atresia
Conclusions CD56(–)CD16(+) NK cells with reduced NK activity may be involved in the bile duct damage in BA, and CD16(+) NK cells expressing CX3CR1 may be attracted by and interact with bile ducts expressing CX3CL1.
Source: Journal of Clinical Pathology - January 28, 2013 Category: Pathology Authors: Okamura, A., Harada, K., Nio, M., Nakanuma, Y. Tags: Liver disease, Pancreas and biliary tract, Immunology (including allergy), Hepatitis and other GI infections, Hepatitis (sexual health) Original articles Source Type: research

The clinical and cost-effectiveness of the BRinging Information and Guided Help Together (BRIGHT) intervention for the self-management support of people with stage 3 chronic kidney disease in primary care: study protocol for a randomized controlled trial
We describe the methods of the patient-level randomized controlled trial.DiscussionThe management of chronic kidney disease is a developing area of research. The BRinging Information and Guided Help Together (BRIGHT) trial aims to provide evidence that a complementary package of support for people with vascular disease that targets both clinical and social need broadens the opportunities of self-management support by addressing problems related to social disadvantage.Trial registration: Trial registration reference: ISRCTN45433299
Source: Trials - January 28, 2013 Category: Journals (General) Authors: Christian BlickemTom BlakemanAnne KennedyPeter BowerDavid ReevesCaroline GardnerVictoria LeeCarolyn Chew-GrahamGerry RichardsonHelen BrooksShoba DawsonRahena MossabirPraksha JariwalaAngela SwallowEvan KontopantelisHannah GaffneyNicola SmallEldon SpackmanA Source Type: research

Comparison of survival of patients with metastases from known versus unknown primaries: survival in metastatic cancer
Conclusions: Patients with CUP have poorer survival than patients with known primaries, except those with brain and respiratory system metastases. Of CUP sites, liver metastases have the worst prognosis. Survival in CUP was comparable to that in metastatic lung cancer. The aggressive behavior of CUP may be due to initial immunosuppression and immunoediting which may allow accumulation of mutations. Upon escape from the suppressed state an unstoppable tumor spread ensues. These novel data on the epidemiology of the metastatic process at the population level demonstrated large survival differences in organ defined metastases...
Source: BMC Cancer - January 28, 2013 Category: Cancer & Oncology Authors: Matias RiihimäkiHauke ThomsenAkseli HemminkiKristina SundquistKari Hemminki Source Type: research

Mesenchymal stem cells contribute to endogenous FVIII:c production
Abstract Besides the liver, it has been difficult to identify which organ(s) and/or cellular component(s) contribute significantly to the production of human FVIII:c (FVIII). Thus far, only endothelial cells have been shown to constitute a robust extrahepatic source of FVIII, possibly explaining both the diverse presence of FVIII mRNA in the body, and the observed increase in FVIII levels during liver failure. Here, we investigate whether human mesenchymal stem cells (MSC), ubiquitously present in different organs, could also contribute to FVIII production. MSC isolated from human lung, liver, brain, and bone marrow expres...
Source: Journal of Cellular Physiology - January 28, 2013 Category: Cytology Authors: Chad Sanada, Chung‐Jung Kuo, Evan J. Colletti, Melisa Soland, Saloomeh Mokhtari, Mary Ann Knovich, John Owen, Esmail D. Zanjani, Christopher D. Porada, Graça Almeida‐Porada Tags: Original Research Article Source Type: research

Molecular Diagnosis of T Cell‐Mediated Rejection in Human Kidney Transplant Biopsies
This study developed a molecular test for T cell‐mediated rejection. We used microarray results from 403 kidney transplant biopsies to derive a classifier assigning T cell‐mediated rejection scores to all biopsies, and compared these with histologic assessments. The score correlated with histologic lesions of T cell‐mediated rejection (infiltrate, tubulitis). The accuracy of the classifier for the histology diagnoses was 89%. Very high and low molecular scores corresponded with unanimity among three pathologists on the presence or absence of T cell‐mediated rejection, respectively. The molecular score had low sensi...
Source: American Journal of Transplantation - January 28, 2013 Category: Transplant Surgery Authors: J. Reeve, J. Sellarés, M. Mengel, B. Sis, A. Skene, L. Hidalgo, D. de Freitas, K. S. Famulski, P. F. Halloran Tags: Original Article Source Type: research

Kidney Transplants With Progressing Chronic Diseases Express High Levels of Acute Kidney Injury Transcripts
This study investigated the significance of this signal in transplants with other conditions, including rejection and recurrent disease. The injury signal was elevated in biopsies in many different conditions, including T cell‐mediated rejection and potentially progressive diseases such as antibody‐mediated rejection and glomerulonephritis. A high injury signal correlated with poor function and with inflammation in areas of fibrosis, but not with fibrosis without inflammation. In multivariate survival analysis, the injury signal in late kidney transplant biopsies strongly predicted future graft loss, similar to a publi...
Source: American Journal of Transplantation - January 28, 2013 Category: Transplant Surgery Authors: K. S. Famulski, J. Reeve, D. G. de Freitas, C. Kreepala, J. Chang, P. F. Halloran Tags: Original Article Source Type: research

Human Monoclonal Antibody MBL‐HCV1 Delays HCV Viral Rebound Following Liver Transplantation: A Randomized Controlled Study
Rapid allograft infection complicates liver transplantation (LT) in patients with hepatitis C virus (HCV). Pegylated interferon‐α and ribavirin therapy after LT has significant toxicity and limited efficacy. The effect of a human monoclonal antibody targeting the HCV E2 glycoprotein (MBL‐HCV1) on viral clearance was examined in a randomized, double‐blind, placebo‐controlled pilot study in patients infected with HCV genotype 1a undergoing LT. Subjects received 11 infusions of 50 mg/kg MBL‐HCV1 (n = 6) or placebo (n = 5) intravenously with three infusions on day of transplant, a single infusion on days 1 through 7...
Source: American Journal of Transplantation - January 28, 2013 Category: Transplant Surgery Authors: R. T. Chung, F. D. Gordon, M. P. Curry, T. D. Schiano, S. Emre, K. Corey, J. Markmann, M. Hertl, J. J. Pomposelli, E. A. Pomfret, S. Florman, M. Schilsky, T. J. Broering, R. W. Finberg, G. Szabo, P. D. Zamore, U. Khettry, G. J. Babcock, D. M. Ambrosino, B Tags: Brief Communication Source Type: research

Galectin‐1 Prolongs Survival of Mouse Liver Allografts From Flt3L‐Pretreated Donors
This study was to investigate the role of galectin‐1 in spontaneous tolerance of liver allografts in mice, and to evaluate the therapeutic effects of galectin‐1 on liver allograft rejection induced by donor Flt3L pretreatment. Blockade of the galectin‐1 pathway via neutralizing antigalectin‐1 mAb did not affect survival of the liver allografts from B6 donors into C3H recipients. Administration of rGal‐1 significantly prolonged survival of liver allografts from Flt3L‐pretreated donors and ameliorated Flt3L‐triggered liver allograft rejection. This effect was associated with increased apoptosis of T cells in bo...
Source: American Journal of Transplantation - January 28, 2013 Category: Transplant Surgery Authors: Y. Ye, S. Yan, G. Jiang, L. Zhou, H. Xie, X. Xie, X. Yu, Y. Ding, J. Tian, Y. Dai, S. Zheng Tags: Original Article Source Type: research

Identification of transcription factors associated with castration‐resistance: Is the serum responsive factor a potential therapeutic target?
CONCLUSIONSRF is a key TF by which resistant cells survive with depleted levels of androgens representing a target for therapeutic manipulation. Prostate © 2013 Wiley Periodicals, Inc.
Source: The Prostate - January 28, 2013 Category: Urology & Nephrology Authors: Maria Prencipe, Stephen F. Madden, Amanda O'Neill, Gillian O'Hurley, Aedin Culhane, Darran O'Connor, Helmut Klocker, Elaine W. Kay, William M. Gallagher, William R. Watson Tags: Original Article Source Type: research

Effects of PFM rehabilitation on PFM function and morphology in older women
ConclusionsThis study indicated that improvements in urine leakage were produced along with improvements in PFM co‐ordination (demonstrated by the increased number of rapid PFM contractions and the earlier PFM activation when coughing), motor‐control, pelvic organ support. Neurourol. Urodynam. © 2013 Wiley Periodicals, Inc.
Source: Neurourology and Urodynamics - January 28, 2013 Category: Urology & Nephrology Authors: Stéphanie J. Madill, Stéphanie Pontbriand‐Drolet, An Tang, Chantale Dumoulin Tags: Original Clinical Article Source Type: research

Dual involvements of cyclooxygenase and nitric oxide synthase expressions in ketamine‐induced ulcerative cystitis in rat bladder
ConclusionsKetamine treatment affected bladder tissues by enhancing interstitial fibrosis and accelerating macrophages infiltration. Ketamine also initiated the up‐regulations of COX‐2 and iNOS and eNOS expressions. These up‐regulated enzymes might play an important role in contributing to ketamine‐induced alterations in micturition patterns and ulcerative cystitis. Neurourol. Urodynam. © 2013 Wiley Periodicals, Inc.
Source: Neurourology and Urodynamics - January 28, 2013 Category: Urology & Nephrology Authors: Shu‐Mien Chuang, Keh‐Min Liu, Yi‐Lun Li, Mei‐Yu Jang, Hei‐Hwa Lee, Wen‐Jeng Wu, Wei‐Chiao Chang, Robert M. Levin, Yung‐Shun Juan Tags: Original Basic Science Article Source Type: research

Nocturia and overnight polysomnography in Parkinson disease
ConclusionsThese results verify objectively that PD patients with nocturia have poor sleep. Furthermore, among individuals with comparable levels of reported nocturia, higher bother is associated with poorer sleep as defined on PSG. Neurourol. Urodynam. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.
Source: Neurourology and Urodynamics - January 28, 2013 Category: Urology & Nephrology Authors: Camille P. Vaughan, Jorge L. Juncos, Lynn Marie Trotti, Theodore M. Johnson, Donald L. Bliwise Tags: Original Clinical Article Source Type: research

Analysis of the surgical learning curve using the cumulative sum (CUSUM) method
ConclusionCUSUM analysis is an underused tool for the analysis of surgical competence. The learning curve for retropubic sling surgery is variable and may be longer than is often acknowledged. We suggest the focus of surgical training should move away from absolute numbers to look at training in an individualized manner. Neurourol. Urodynam. © 2013 Wiley Periodicals, Inc.
Source: Neurourology and Urodynamics - January 28, 2013 Category: Urology & Nephrology Authors: Turlough Maguire, Christopher J. Mayne, Tim Terry, Douglas G. Tincello Tags: Original Clinical Article Source Type: research

Comparison of questionnaires used for the evaluation of patients with chronic pelvic pain
ConclusionWhen the most used questionnaires for QoL assessment in patients with CPPS are compared, very different results can be found. This indicates that results from one questionnaire cannot be used for overall conclusions concerning pain intensity and QoL. For bladder symptoms the results seem to correspond better. To develop one generally accepted questionnaire would facilitate the interpretation and comparison of data in this condition. Neurourol. Urodynam. © 2013 Wiley Periodicals, Inc.
Source: Neurourology and Urodynamics - January 28, 2013 Category: Urology & Nephrology Authors: Jörgen Quaghebeur, Jean‐Jacques Wyndaele Tags: Original Basic Science Article Source Type: research

High‐risk human papillomaviruses in two different primary tumors in the same patient
Abstract: Two cases of patients with high‐risk human papillomavirus‐related squamous cell carcinomas of the penis are reported. In both patients, a second high‐risk human papillomavirus‐related squamous cell carcinoma, of the same type (genotype 16), was detected: a carcinoma of the oropharynx 2 years after treatment of the squamous cell carcinomas of the penis in the first patient, and a carcinoma of the esophagus 1 year after the treatment of the squamous cell carcinomas of the penis in the second patient. To the best of our knowledge, this is the first time that multiple human papillomavirus‐related tumors ...
Source: International Journal of Urology - January 28, 2013 Category: Urology & Nephrology Authors: Mânlio Tasso Oliveira Mota, Jane Lopes Bonilha, Bruno Miziara Rosa, José Germano Ferraz Arruda, Fernando Augusto Soares, José Vassallo, Marilia Freitas Calmon, Paula Rahal Tags: Case Report Source Type: research

Assessment of cardiometabolic risk and prevalence of meeting treatment guidelines among patients with type 2 diabetes stratified according to their use of insulin and/or other diabetic medications: results from INSPIRE ME IAA
ConclusionResidual cardiometabolic risk exists among patients with type 2 diabetes characterized by elevated VAT and liver fat.
Source: Diabetes, Obesity and Metabolism - January 28, 2013 Category: Endocrinology Authors: Jessica Smith, Julie‐Anne Nazare, Anne‐Laure Borel, Pablo Aschner, Philip J Barter, Luc Van Gaal, Yuji Matsuzawa, Takashi Kadowaki, Robert Ross, Claire Brulle‐Wohlhueter, Natalie Alméras, Steven M Haffner, Beverley Balkau, Jean‐Pierre Després Tags: Original Paper Source Type: research

Identification of a distinct mutation spectrum in the SMPD1 gene of Chinese patients with acid sphingomyelinase-deficient Niemann-Pick disease
Conclusions: The Chinese population may have a comparably high incidence of sphingomyelinase-deficient Niemann-Pick disease type A. This study has identified some novel genotype and phenotype correlations in this rare and devastating disorder.
Source: Orphanet Journal of Rare Diseases - January 28, 2013 Category: Internal Medicine Authors: Huiwen ZhangYu WangZhuwen GongXiaoyan LiWenjuan QiuLianshu HanJun YeXuefan Gu Source Type: research

Cost‐effectiveness of single‐dose tamsulosin and dutasteride combination therapy compared with tamsulosin monotherapy in patients with benign prostatic hyperplasia in the UK
Conclusion Single‐dose combination dutasteride/tamsulosin therapy has a high probability of being cost‐effective in comparison to tamsulosin monotherapy in the UK‘s NHS.
Source: BJU International - January 28, 2013 Category: Urology & Nephrology Authors: Anna Walker, Scott Doyle, John Posnett, Manjit Hunjan Tags: Functional Urology Source Type: research

Contraction of the anterior prostate is required for the initiation of micturition
Conclusions Our functional and biometric in vitro analyses of prostate tissue showed sufficient contractile strength of the ventral prostate to induce a shortening of the organ as seen on rtMRI. There was significant higher contractile strength in the ventral prostate than in the dorsal prostate or the proximal urethra. The consistency of in vivo and in vitro results underlines the significance of the ventral prostate for the initiation of normal micturition.
Source: BJU International - January 28, 2013 Category: Urology & Nephrology Authors: Yasemin Hocaoglu, Karin Herrmann, Sebastian Walther, Martin Hennenberg, Christian Gratzke, Ricarda Bauer, Christian Stief, Alexander Roosen Tags: Functional Urology Source Type: research

Fourteen‐year oncological and functional outcomes of high‐intensity focused ultrasound in localized prostate cancer
Conclusions The study demonstrates the efficacy and safety of HIFU for localized PCa. HIFU is a therapeutic option for patients of advanced age, in the low‐ or intermediate‐risk groups, and with a life expectancy of ∼10 years.
Source: BJU International - January 28, 2013 Category: Urology & Nephrology Authors: Roman Ganzer, Hans‐Martin Fritsche, Andreas Brandtner, Johannes Bründl, Daniel Koch, Wolf F. Wieland, Andreas Blana Tags: Urological Oncology Source Type: research

Longitudinal evaluation of hepatic lipid deposition and composition in ob/ob and ob/+ control mice
The objective of this study was to quantitatively assess lipid deposition (fractional lipid mass, fLM) and composition (fraction of polyunsaturated lipids, fPUL and mean chain length, MCL) in livers of ob/ob mice, a genetic model of obesity and mild diabetes, and ob/+ heterozygous control animals in a noninvasive manner using 1H‐MRS at 9.4T. For accurate quantification, intensity values were corrected for differences in T2 values while T1 effects were considered minimal due to the long TR values used. Values of fLM, fPUL and MCL were derived from T2‐corrected signal intensities of lipids and water resonance. Hepatic li...
Source: NMR in Biomedicine - January 28, 2013 Category: Radiology Authors: Qiong Ye, Carsten Friedrich Danzer, Alexander Fuchs, Divya Vats, Christian Wolfrum, Markus Rudin Tags: Research article Source Type: research

Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene
Conclusions: MMP-9 may play an important role in the mechanism of pathological changes during theearlier period of CAN.Virtual SlidesThe virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/1582313332832700
Source: Diagnostic Pathology - January 28, 2013 Category: Pathology Authors: Dongfeng GuYanling ShiYanan DingXinyu LiuHequn Zou Source Type: research

Ginseng/multivitamin/raltegravir interaction: First report an interaction, leading to liver injury: case report
Source: Reactions - January 27, 2013 Category: Drugs & Pharmacology Tags: Short communication Source Type: research

MicroRNA-122-dependent and -independent replication of Hepatitis C Virus in Hep3B human hepatoma cells.
Abstract The study of Hepatitis C Virus (HCV) has benefitted from the use of the Huh7 cell culture system, but until recently there were no other widely used alternatives to this cell line. Here we render another human hepatoma cell line, Hep3B, permissive to the complete virus life cycle by supplementation with the liver-specific microRNA miR-122, known to aid HCV RNA accumulation. When supplemented, Hep3B cells produce J6/JFH-1 virus titres indistinguishable from those produced by Huh7.5 cells. Interestingly, we were able to detect and characterize miR-122-independent replication of di-cistronic replicons in Hep3...
Source: Virology - January 27, 2013 Category: Virology Authors: Thibault PA, Huys A, Dhillon P, Wilson JA Tags: Virology Source Type: research

Dog nectin-4 is an epithelial cell receptor for canine distemper virus that facilitates virus entry and syncytia formation.
Abstract Canine distemper virus (CDV) was shown to use dog nectin-4 as a receptor to gain entry into epithelial cells. RNA from dog placenta or MDCK kidney cells was isolated and cDNAs were prepared. Two splice variants of dog nectin-4 were identified. A deletion of 25 amino acids was found in the cytoplasmic domain of dog nectin-4 from MDCK cells, corresponding to a splice variant that is also seen in murine nectin-4, and did not affect its role as a receptor. Both dog nectin-4 and human nectin-4 could function as an entry factor for CDV containing an EGFP reporter gene. Inhibition of dog nectin-4 expression by RN...
Source: Virology - January 27, 2013 Category: Virology Authors: Noyce RS, Delpeut S, Richardson CD Tags: Virology Source Type: research

Immunological monitoring after pancreas transplantation.
Abstract PURPOSE OF REVIEW: After switching from bladder to enteric drainage, pancreas graft monitoring, particularly after solitary transplantation, has become an important issue. The aim of this work was to systematically review the relevant literature with regard to various biomarkers, imaging techniques, and pathologic evaluation of allograft tissue. RECENT FINDINGS: More recent studies including graft histology demonstrate the low specificity of pancreatic enzymes as a marker of acute rejection. On the other hand, most blood and serum markers are indicative of an activated immune status rather than rejecti...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Margreiter C, Pratschke J, Margreiter R Tags: Curr Opin Organ Transplant Source Type: research

The role of mesenchymal stromal cells in chronic transplant rejection after solid organ transplantation.
Abstract PURPOSE OF REVIEW: Despite impressive improvements in short-term survival after solid organ transplantation (SOT), the long-term outcome remains an important challenge. In recent years, it has become evident that ongoing alloreactivity, cellular and/or humoral, may play a critical role in the premature loss of kidney allografts. Clinical trials with mesenchymal stromal cells (MSCs) are currently underway for the treatment of various inflammatory disorders. Due to their immunosuppressive and reparative properties MSC immunotherapy constitutes an attractive intervention for chronic rejection, while avoiding ...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Reinders ME, Rabelink TJ, de Fijter JW Tags: Curr Opin Organ Transplant Source Type: research

Machine perfusion in organ transplantation: a tool for ex-vivo graft conditioning with mesenchymal stem cells?
This article aims to review the current knowledge on machine perfusion of the various solid thoracic and abdominal organs, and to discuss the new possibility of conditioning and treating grafts with mesenchymal stem cells (MSCs) during machine perfusion. RECENT FINDINGS: Different methods of machine perfusion have been described varying among organs in temperature and composition of perfusate. Commercial devices have recently become available for machine perfusion of all organs, with the largest clinical experience acquired in kidney and lung transplantation. Clinical studies are ongoing for liver, heart, and pancreas....
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Van Raemdonck D, Neyrinck A, Rega F, Devos T, Pirenne J Tags: Curr Opin Organ Transplant Source Type: research

Rethinking the multiple roles of B cells in organ transplantation.
Abstract PURPOSE OF REVIEW: To discuss the B-cell diverse functions in organ transplantation, highlighting the emerging debate on the role of regulatory B cells (Bregs). We underscore the need to re-examine and integrate data on B-cell functional activities, aiming to discriminate their regulatory (REG) and inflammatory (INFLAMMA) functions and to translate this knowledge for the development of novel immunomodulatory therapeutic strategies and to rethink the current ones. RECENT FINDINGS: Data from both experimental models and clinical trials point that B cells of various phenotypes have immunoregulatory activi...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Coelho V, Saitovitch D, Kalil J, Silva HM Tags: Curr Opin Organ Transplant Source Type: research

Preoperative risk evaluation: where is the limit for recipients of a pancreatic graft?
Preoperative risk evaluation: where is the limit for recipients of a pancreatic graft? Curr Opin Organ Transplant. 2013 Feb;18(1):97-101 Authors: Eller K, Kniepeiss D, Rosenkranz AR Abstract PURPOSE REVIEW: Pancreas transplantation is an accepted treatment strategy that can result in normalization of blood glucose, but this must be weighed against the risks of a surgical procedure and subsequent immunosuppression. To improve the risk/benefit ratio, pancreas transplantation is typically performed in end-stage renal disease patients who are undergoing simultaneous kidney transplantation or who previously rece...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Eller K, Kniepeiss D, Rosenkranz AR Tags: Curr Opin Organ Transplant Source Type: research

Ischemia-reperfusion injury: beneficial effects of mesenchymal stromal cells.
Ischemia-reperfusion injury: beneficial effects of mesenchymal stromal cells. Curr Opin Organ Transplant. 2013 Feb;18(1):34-43 Authors: Souidi N, Stolk M, Seifert M Abstract PURPOSE OF REVIEW: Organ transplantation and other major surgeries are impacted by ischemia-reperfusion injury (IRI). Mesenchymal stromal cells (MSCs) recently became an attractive alternative therapeutic tool to combat IRI. The present review highlights the effects of MSCs in the preclinical animal models of IRI and clinical trials, and explains their potential modes of action based on the pathophysiological IRI cascade. RECENT FIN...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Souidi N, Stolk M, Seifert M Tags: Curr Opin Organ Transplant Source Type: research

Mesenchymal stromal cells to promote solid organ transplantation tolerance.
Abstract PURPOSE OF REVIEW: Mesenchymal stromal cells (MSCs) possess unique immunomodulatory features. MSCs dampen effector T-cell response while promoting the emergence of regulatory T cells. By skewing this balance, MSC could represent the ideal strategy for tolerance induction in organ transplantation. Here we review recent evidence on the efficacy of MSC-based therapy in experimental models of solid organ transplantation as well as the early clinical experiences in kidney transplantation. RECENT FINDINGS: MSC infusion in experimental models of solid organ transplantation resulted in a Treg-mediated toleranc...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Casiraghi F, Perico N, Remuzzi G Tags: Curr Opin Organ Transplant Source Type: research

Follow-up of secondary diabetic complications after pancreas transplantation.
Abstract PURPOSE OF REVIEW: Successful pancreas transplantation restores physiologic glycemic and metabolic control. Its effects on overall patient survival (especially for simultaneous pancreas-kidney transplantation) are clear-cut. We herein review the available literature to define the impact of pancreas transplantation on chronic complications of diabetes mellitus. RECENT FINDINGS: With longer-term follow-up, wider patient populations, and more accurate investigational tools (clinical and functional tests, noninvasive imaging, histology, and molecular biology), growing data show that successful pancreas tra...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Boggi U, Rosati CM, Marchetti P Tags: Curr Opin Organ Transplant Source Type: research

New perspectives in transplantation through intravital microscopy imaging.
Abstract PURPOSE OF REVIEW: Rejection of transplanted organs is a complex and highly dynamic immune process. Two-photon laser-scanning intravital microscopy (LSIM) allows for real-time, deep tissue, high-resolution imaging in physiological conditions. The recent application of this technology to study organ rejection started to provide a clearer picture of the spatiotemporal immunological dynamics of organ rejection. RECENT FINDINGS: To date, LSIM has been applied to transplanted skin, islet, and kidney in mice, as well to constantly moving organs such as transplanted lung and heart. To characterize the dynamic...
Source: Current Opinion in Organ Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Camirand G Tags: Curr Opin Organ Transplant Source Type: research

Prognostic Significance of Patterns of Seminal Vesicle Invasion of Prostate Cancer
ConclusionsThe prognosis of patients with SVI is not uniformly poor. Invasion of the SV mucosa portends a higher risk of recurrence than invasion of the muscle wall alone. There is no evidence that other histopathological features of SVI need to be reported.Seminal vesicle invasion (SVI) of prostatic carcinoma was defined by the 2009 International Society of Urological Pathology (ISUP) consensus conference on Handling and Staging of Radical Prostatectomy Specimens as cancer invading the muscular wall of the extraprostatic part of the seminal vesicle (SV) 1. SVI is seen in 3.1% 2 – 9.2% 3 of radical prostatectomy (RP) spe...
Source: Histopathology - January 27, 2013 Category: Pathology Authors: Anna Kristiansen, Fredrik Wiklund, N Peter Wiklund, Lars Egevad Tags: Original Article Source Type: research

Identification of human epididymis protein-4 as a fibroblast-derived mediator of fibrosis
Nature Medicine 19, 227 (2013). doi:10.1038/nm.2989 Authors: Valerie S LeBleu, Yingqi Teng, Joyce T O'Connell, David Charytan, Gerhard A Müller, Claudia A Müller, Hikaru Sugimoto & Raghu Kalluri The functional contribution of myofibroblasts in fibrosis is not well understood. Using a new genetic mouse model to track and isolate myofibroblasts, we performed gene expression profiling followed by biological validation to identify HE4 (encoding human epididymis protein 4, also known as WAP 4-disulfide core domain-2 or Wfdc2) as the most upregulated gene in fibrosis-associated myofibroblasts. The HE4 gene ...
Source: Nature Medicine - January 27, 2013 Category: Journals (General) Authors: Valerie S LeBleuYingqi TengJoyce T O'ConnellDavid CharytanGerhard A MüllerClaudia A MüllerHikaru SugimotoRaghu Kalluri Tags: Letter Source Type: research

Selenium Supplementation Modulates Zinc Levels and Antioxidant Values in Blood and Tissues of Diabetic Rats Fed Zinc-Deficient Diet.
In conclusion, selenium supplementation presumably acting as an antioxidant led to an improvement of insulin activity, significantly reducing the severity of zinc deficiency in diabetes. PMID: 23354544 [PubMed - as supplied by publisher]
Source: Biological Trace Element Research - January 27, 2013 Category: Biology Authors: Fatmi W, Kechrid Z, Nazıroğlu M, Flores-Arce M Tags: Biol Trace Elem Res Source Type: research

[Long-term effects of osteopathic treatment of chronic prostatitis with chronic pelvic pain syndrome : A 5-year follow-up of a randomized controlled trial and considerations on the pathophysiological context.]
CONCLUSIONS: Due to the sustainability of osteopathic treatment and the low amount of time involved, osteopathy should be taken seriously as a treatment for patients with CP/CPPS. Furthermore, due to the osteopathic treatment the patients learnt to alleviate or even eliminate their own symptoms in treating themselves. In order to help many other affected persons out of their dilemma it would be desirable if more urologists and internists would become acquainted with osteopathy in order to be able to offer this to patients at an early stage. Further studies with larger numbers of patients should be carried out to con...
Source: Der Urologe. Ausg. A - January 27, 2013 Category: Urology & Nephrology Authors: Marx S, Cimniak U, Rütz M, Resch KL Tags: Urologe A Source Type: research

[Freiburg index of patient satisfaction : Interdisciplinary validation of a new psychometric questionnaire to describe treatment-related patient satisfaction.]
CONCLUSIONS: The FIPS constitutes an interdisciplinary validated questionnaire to evaluate treatment-related patient satisfaction which can be used to objectify and compare results from clinical studies and quality in patient care. Colleagues of English-speaking countries are invited to participate in the validation of the hereby presented English version. PMID: 23354559 [PubMed - as supplied by publisher]
Source: Der Chirurg - January 27, 2013 Category: Surgery Authors: Miernik A, Farin E, Kuehhas FE, Karcz WK, Keck T, Wengenmayer T, Kollum M, Bach C, Buchholz N, Schoenthaler M Tags: Chirurg Source Type: research

Antitumor and antiangiogenic active dendrimer/5-fluorouracil conjugates.
Abstract Antitumor and antiangiogenic active dendritic compounds conjugated with 5-fluorouracil (5-FU) were synthesized and characterized. First, the core structure based on ascorbic acid and dicarboxylic acid was synthesized, amidated with ethylenediamine. For the synthesis of higher generated dendrimer, it was reacted further with methyl acrylate to increase molecular weight through Michael addition reaction, followed by the continuous repeating amidation reaction, finally, used to synthesize covalently bound dendrimer/5-FU conjugates. The in vitro cytotoxicities of the conjugates was evaluated with mouse mammary...
Source: Cell Research - January 27, 2013 Category: Cytology Authors: Lee SM, Bala YS, Lee WK, Jo NJ, Chung I Tags: J Biomed Mater Res A Source Type: research

Donation after cardiac death: results of the SUMMA 112 – Hospital 12 de Octubre Program
ConclusionsDACD is an adequate source of organs for kidney transplantation. Our functional and survival results are encouraged in the short term, although further work is required to increase the program's benefits.
Source: Clinical Transplantation - January 27, 2013 Category: Transplant Surgery Authors: Natalia Miranda‐Utrera, José Medina‐Polo, Manuel Pamplona, Federico la Rosa, Alfredo Rodríguez, José M. Duarte, Juan B. Passas, Alonso Mateos‐Rodríguez, Rafael Díaz, Amado Andrés Tags: Original Article Source Type: research