MedWorm: Epidermoid Carcinoma

Effectiveness of modified radical neck dissection and postoperative radiotherapy.

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Fri, 05 Mar 2010 00:00:00 +0100

DISCUSSION: MRND in association with PORT is an effective treatment in patients with advanced head and neck epidermoid carcinoma staged N2 and N3. PMID: 20207384 [PubMed - as supplied by publisher] (Source: Revue de Stomatologie et de Chirurgie Maxillo-Faciale)

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Andrographolide regulates epidermal growth factor receptor and transferrin receptor trafficking in epidermoid carcinoma (A-431) cells

British Journal of Pharmacology — Wed, 03 Mar 2010 00:00:00 +0100

Conclusion and implications: This study sheds light on how andrographolide may affect receptor trafficking by inhibiting receptor movement from the late endosomes to lysosomes. The down-regulation of EGFR from the cell surface also indicates a new mechanism by which andrographolide may induce cancer cell death. (Source: British Journal of Pharmacology)

A Review of Penile Cancer

Advances in Urology — Tue, 16 Feb 2010 15:49:20 +0100

Conclusion. Penile carcinoma is a rare pathology which affects elderly persons and is diagnosed late. (Source: Advances in Urology)

gamma-Glutamyl 16-diaminopropane derivative of vasoactive intestinal peptide: a potent anti-oxidative agent for human epidermoid cancer cells.

Amino Acids — Wed, 10 Feb 2010 00:00:00 +0100

Authors: Stiuso P, Giuberti G, Lombardi A, Dicitore A, Limongelli V, Cartenì M, Abbruzzese A, Caraglia M We previously demonstrated that the gamma-glutamyl 16 amine derivative of vasoactive intestinal peptide (VIP) acts as structural VIP agonist with affinity and potency higher than VIP. Herein, we have evaluated the effects of VIP and gamma-Gln16-diaminopropane derivative of VIP (VIP-DAP3) on the proliferation and protection from oxidative stress induced by hydrogen peroxide (H(2)O(2)) on epidermoid carcinoma cell lines. We have found that 10(-11) M VIP-DAP3 completely antagonized the inhibition induced by H(2)O(2) on both cell proliferation and S-phase distribution while these effects were only partially antagonized by equimolar concentrations of VIP. Moreover, both oxidative st...

Complex tracheal lesion: correction with an intercostal muscle pedicle flap

Jornal Brasileiro de Pneumologia — Sat, 30 Jan 2010 15:33:10 +0100

We report the case of a patient diagnosed with epidermoid carcinoma in the distal third of the esophagus. The patient was submitted to esophagectomy by video-assisted thoracoscopy and laparoscopy. During the operation, the left main bronchus was injured, and this required immediate surgical correction. In the postoperative period, the patient presented with acute respiratory failure and profuse air leak through the thoracic drains and through the cervical surgical wound. The patient underwent a second surgical procedure, during which a large lesion was discovered in the membranous wall of the trachea. The lesion was corrected with an intercostal muscle pedicle flap. (Source: Jornal Brasileiro de Pneumologia)

Integrative molecular characterization of head and neck cancer cell model genomes

Head and Neck — Wed, 16 Dec 2009 00:00:00 +0100

Cell lines are invaluable model systems for the investigation of cancer. Knowledge of the molecular alterations that exist within cell models is required to define the mechanisms governing cellular phenotypes.Five tongue squamous cell carcinomas cell lines and 1 submaxillary salivary gland epidermoid carcinoma cell line were analyzed for copy number and mRNA expression by tiling-path DNA microarrays and Agilent Whole Human Genome Oligoarrays, respectively.Integrative analysis of genetic and expression alterations revealed the molecular landscape of each cell line. Molecular results for individual cell lines and across all samples have been summarized and made available for easy reference.Our integrative genomic analyses have defined the DNA and RNA alterations for each individual line. The...

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In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells

Evidence-based Complementary and Alternative Medicine — Tue, 24 Nov 2009 15:14:55 +0100

Propolis is a sticky dark-colored material showing a very complex chemical composition that honeybees collect from plants. It has been used in folk medicine since ancient times, due to several biological properties, such as antimicrobial, anti-inflammatory, antioxidant and immunomodulatory activities, among others. Its antitumor action in vivo and in vitro has also been reported, using propolis extracts or its isolated compounds. The goal of this work was to evaluate propolis's cytotoxic action in vitro on human laryngeal epidermoid carcinoma (Hep-2) cells. These cells were incubated with different concentrations of this bee product for different time periods, and morphology and the number of viable HEp-2 cells analyzed. Data showed that propolis exhibited a cytotoxic effect in vitro again...

Identification of MYO18A as a Novel Interacting Partner of the PAK2/{beta}PIX/GIT1 Complex and Its Potential Function in Modulating Epithelial Cell Migration.

Mol Biol Cell — Wed, 18 Nov 2009 00:00:00 +0100

Authors: Hsu RM, Tsai MH, Hsieh YJ, Lyu PC, Yu JS Monitoring Editor: Jonathan Chernoff The p21-activated kinase (PAK) 2 is known to be involved in numerous biological functions, including the regulation of actin reorganization and cell motility. To better understand the mechanisms underlying this regulation, we herein used a proteomic approach to identify PAK2-interacting proteins in human epidermoid carcinoma A431 cells. We found that MYO18A, an emerging member of the myosin superfamily, is a novel PAK2 binding partner. Using a siRNA knockdown strategy and in vitro binding assay, we discovered that MYO18A binds to PAK2 through the betaPIX/GIT1 complex. Under normal conditions, MYO18A and PAK2 colocalized in lamellipodia and membrane ruffles. Interestingly, knockdown of MYO18A in cells...

Impact of target volumes and radiation technique on loco-regional control and survival for patients with unilateral cervical lymph node metastases from an unknown primary

Radiotherapy and Oncology — Fri, 06 Nov 2009 00:00:00 +0100

Conclusions: Retrospective comparisons between bilateral and unilateral neck radiotherapies did not show differences in terms of loco-regional control and survival. However, patient’s local regional control and survival are significantly improved after 3D-CRT or IMRT. (Source: Radiotherapy and Oncology)

Impact of target volumes and radiation technique on loco-regional control and survival for patients with unilateral cervical lymph node metastases from an unknown primary.

Wed, 04 Nov 2009 00:00:00 +0100

CONCLUSIONS: Retrospective comparisons between bilateral and unilateral neck radiotherapies did not show differences in terms of loco-regional control and survival. However, patient's local regional control and survival are significantly improved after 3D-CRT or IMRT. PMID: 19892420 [PubMed - as supplied by publisher] (Source: Radiotherapy and Oncology : journal of the European Society for Therapeutic Radiology and Oncology)

[Definitive treatment of anal canal carcinoma with radiotherapy: Adverse impact of a pre-radiation resection. A retrospective study of 57 patients treated with curative intent.]

Cancer Radiotherapie — Thu, 22 Oct 2009 00:00:00 +0100

CONCLUSION: Radiotherapy and chemoradiation with cisplatine-based chemotherapy cure a vast majority of patients with epidermoid carcinoma of the anal canal. Therapeutic factors that may interfere with the definition of the target volume and the patients' repositioning may decrease the efficacy of radiotherapy. Pre-radiotherapy surgical resection should be avoided. PMID: 19854092 [PubMed - as supplied by publisher] (Source: Cancer Radiotherapie)

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[TNF-alpha inhibitor etanercept and oral cavity carcinoma.]

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Tue, 13 Oct 2009 23:00:00 +0100

DISCUSSION: The possible development of an oral cavity carcinoma should be taken into account when following a patient under TNF-alpha inhibitor treatment. Anti-TNF treatment has improved the management of patients with severe chronic inflammatory diseases. They allow for a better quality of life. Nevertheless, their immunosuppressive effect should be taken into account when prescribed and during follow-up. PMID: 19836037 [PubMed - as supplied by publisher] (Source: Revue de Stomatologie et de Chirurgie Maxillo-Faciale)

Essential Oil of Artemisia Capillaris Induces Apoptosis in KB Cells via Mitochondrial Stress and Caspase Activation Mediated by MAPK-Stimulated Signaling Pathway

Journal of Food Science — Mon, 12 Oct 2009 23:00:00 +0100

This study examined whether or not the essential oil isolated from A. capillaris induces apoptosis in the human oral epidermoid carcinoma cells along with the possible mechanism(s) of the essential oil-mediated cytotoxicity. The essential oil induced cell death of KB cells through apoptosis, as evidenced by the increased cell population in the sub-G1 phase, the appearance of condensed and/or fragmented nuclei, and the generation of a cleaved PARP product. Treating the cells with the oil also caused changes in the mitochondrial level of the Bcl-2 family proteins such as Bcl-2 and Bax, thereby inducing the release of cytochrome c into the cytosol. Additional experiments using caspase or mitogen-activated protein kinase inhibitors suggested the involvement of the p38/NF-[kappa]B and JNK/Bcl-2...

Is frozen section reliable in transoral CO2 laser-assisted cordectomies?

European Archives of Oto-Rhino-Laryngology — Tue, 29 Sep 2009 04:04:53 +0100

Abstract Endoscopic resection of laryngeal tumors is replacing external approaches. One drawback of endoscopic resection is the difficulty of interpretation of histological specimens because of thermal effect of laser on tissues. Our goal is to assess the reliability of frozen section in this setting by comparing its results with those of routine histology. We, retrospectively, reviewed the charts of all consecutive patients, who underwent cordectomies in our institution between January 2000 and 2008, using the CO2 laser Acublade system (Lumenis, Santa Clara, CA). Age, sex, staging of the tumor, previous treatments, type of cordectomy, frozen section and routine histology results were analyzed. Ninety-seven patients fulfilled the inclusion criteria; 22.7% had severe dyspla...

[11C]Gefitinib ([11C]Iressa): Radiosynthesis, In Vitro Uptake, and In Vivo Imaging of Intact Murine Fibrosarcoma

Molecular Imaging and Biology — Tue, 29 Sep 2009 01:05:47 +0100

Conclusion These results demonstrated the promising potential of [11C]gefitinib to serve as a PET ligand for in vivo imaging of NFSa-bearing mice. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0265-5Authors Ming-Rong Zhang, National Institute of Radiological Sciences Department of Molecular Probes, Molecular Imaging Center 4-9-1 Anagawa, Inage-ku Chiba JapanKatsushi Kumata, National Institute of Radiological Sciences Department of Molecular Probes, Molecular Imaging Center 4-9-1 Anagawa, Inage-ku Chiba JapanAkiko Hatori, National Institute of Radiological Sciences Department of Molecular Probes, Molecular Imaging Center 4-9-1 Anagawa, Inage-ku Chiba JapanNobuhiko Takai, National Institute of Radiological Sciences Heavy-Ion Radiobiology Rese...

EGCG induces apoptosis in human laryngeal epidermoid carcinoma Hep2 cells via mitochondria with the release of apoptosis-inducing factor and endonuclease G

Cancer Letters — Fri, 25 Sep 2009 00:00:00 +0100

Abstract: (−)-Epigallocatechin-3-gallate (EGCG), a major green tea polyphenol, was tested for in vitro cytotoxicity against human laryngeal epidermoid carcinoma of the larynx Hep2 cells. EGCG-induced apoptotic cell death accompanied by a change in the cell cycle. However, EGCG did not result in caspase activation, nor did a caspase inhibitor block cell death. Furthermore, EGCG caused no change in the intracellular levels of reactive oxygen species (ROS). The levels of p53 were increased in the EGCG-treated cells, with a corresponding decrease in Bcl-2 and Bid protein levels as well as an increase in the Bax level. In addition, EGCG induced the cytoplasmic release of cytochrome c from the mitochondria accompanied by a decreased mitochondrial membrane potential, and subsequently upregulate...

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Identification and Characterization of Lutheran Blood Group Glycoprotein as a New Substrate of Membrane-type 1 Matrix Metalloproteinase 1 (MT1-MMP): A SYSTEMIC WHOLE CELL ANALYSIS OF MT1-MMP-ASSOCIATING PROTEINS IN A431 CELLS [Enzyme Catalysis and Regulation]

Journal of Biological Chemistry — Thu, 24 Sep 2009 23:00:00 +0100

Membrane-type 1 matrix metalloproteinase 1 (MT1-MMP) is a potent modulator of the pericellular microenvironment and regulates cellular functions in physiological and pathological settings in mammals. MT1-MMP mediates its biological effects through cleavage of specific substrate proteins. However, our knowledge of MT1-MMP substrates remains limited. To identify new substrates of MT1-MMP, we purified proteins associating with MT1-MMP in human epidermoid carcinoma A431 cells and analyzed them by mass spectrometry. We identified 163 proteins, including membrane proteins, cytoplasmic proteins, and functionally unknown proteins. Sixty-four membrane proteins were identified, and they included known MT1-MMP substrates. Of these, eighteen membrane proteins were selected, and we confirmed their asso...

Termination of tyrphostin AG1478 application results in different recovery of EGF receptor tyrosine residues 1045 and 1173 phosphorylation in A431 cells.

Cell Biology International — Fri, 18 Sep 2009 00:00:00 +0100

Authors: Kondratov KA, Chernorudskiy AL, Amosova AP, Kornilova ES Tyrphostin AG1478 is known as a specific and reversible inhibitor of tyrosine kinase (TK) activity of the EGF receptor (EGFR). It is attractive as an anti-cancer agent for cancers with elevated EGFR TK levels. However, post-application effects of AG1478 are not well studied. We have analyzed EGFR phosphorylation after termination of AG1478 application using human epidermoid carcinoma A431 cells. It was found that AG1478 inhibitory action is fast but not fully reversible: removal of tyrphostin resulted in incomplete restoration of the overall EGFR phosphorylation. Analyzing the state of two individual autophosphorylation sites of internalized EGFR, Y1045 and Y1173, we demonstrated that phosphorylation of Y1173 involved in...

Reconstruction of the pulmonary artery for lung cancer: Long-term results

The Journal of Thoracic and Cardiovascular Surgery — Wed, 09 Sep 2009 23:00:00 +0100

Conclusions: Pulmonary artery reconstruction is safe, with excellent long-term survival. Our results support this technique as an effective option for patients with lung cancer. (Source: The Journal of Thoracic and Cardiovascular Surgery)

[6]-Gingerol induces reactive oxygen species regulated mitochondrial cell death pathway in human epidermoid carcinoma A431 cells.

Chemico-Biological Interactions — Wed, 19 Aug 2009 22:34:25 +0100

Authors: Nigam N, Bhui K, Prasad S, George J, Shukla Y Since skin cancer incidence and prevalence is constantly rising up the charts despite all efforts, search for newer, better agents for protection and treatment is required. Ginger (Zingiber officinale Roscoe), a monocotyledonous herb, is widely used as a herbal medicine, given the presence of homologous phenolic ketones, of which [6]-gingerol is the major one. The quantity of [6]-gingerol in the fresh ginger rhizome was found to be 104-965 microg/g in common varieties of ginger available in Indian market. Herein, [6]-gingerol was assessed for its anti-apoptotic effects in human epidermoid carcinoma A431 cells. [6]-Gingerol treatment exhibited considerable cytotoxicity as indicated by growth inhibition of A431 cells mediated via gen...

Immunohistochemical expression of ubiquitin and telomerase in cervical cancer

Virchows Archiv — Wed, 19 Aug 2009 08:30:12 +0100

Abstract Ubiquitin and telomerase immunohistochemical expression patterns in cervical cancer were compared with normal cervical tissue samples. Eighty-one cervical cancer cases and 22 normal exo–endocervical tissue were examined with polyclonal antibody for ubiquitin and 44G12 clone for telomerase using tissue microarrays. The results were interpreted using a semiquantitative scale The average age of patients was 50.67 years. The most frequent histological types were moderately differentiated epidermoid carcinoma (43.5%), according to the degree of differentiation, and endocervical adenocarcinoma (42.1%). Immunohistochemical findings were as follows: 98.7% of cervical cancers showed immunoexpression for ubiquitin and 52.6% for telomerase. Statistically significant d...

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Reproducibility of the kinetic analysis of 3′-deoxy-3′-[18F]fluorothymidine positron emission tomography in mouse tumor models

Nuclear Medicine and Biology — Wed, 29 Jul 2009 23:00:00 +0100

Abstract: Objectives: We assessed the reproducibility of the kinetic analysis of 3′-deoxy-3′-[18F]fluorothymidine (FLT) positron emission tomography (PET) in A431 human epidermoid carcinoma and murine Lewis lung carcinoma (LLC) tumor models.Methods: We injected 7.4 MBq of FLT (n=10 for each group) and acquired 2-h dynamic PET images. A second scan was performed 1 day later. We calculated standardized uptake value (SUV), kinetic rate constants, volume of distribution of phosphorylated FLT (Vdm), net influx constant (KFLT-CA) and influx constant by Patlak graphical analysis (KFLT-PA). The percent difference between measurements of a parameter was calculated to compare the reproducibilities of different parameters.Results: FLT phosphorylation was higher in mice with A431 tumors than in mi...

Bioactivity-Guided Isolation of Cytotoxic Sesquiterpenes and Flavonoids from Anthemis ruthenica

Planta Medica — Wed, 29 Jul 2009 10:52:38 +0100

Planta MedDOI: 10.1055/s-0029-1185942AbstractA new eudesmanolide sesquiterpene, sivasinolide 6--angelate (), was isolated from the aerial parts of together with the known compounds chrysanin (), tanacin (), 3-hydroxycostunolide (), centauridin (), and centaureidin (). The compounds were obtained by means of bioactivity-guided fractionation from the CHCl extract of the herb, which displayed high cytotoxic activity. The structures were determined by UV, HR‐ESI‐MS, and high-field 1D and 2D NMR spectral analyses, affording complete H- and C‐NMR assignments for all compounds. The cytotoxic activities of the isolated sesquiterpenes and flavonoids were assessed against cervical adenocarcinoma HeLa, breast adenocarcinoma MCF7, and skin epidermoid carcinoma A431 cells using ...

Up-regulation of the tight-junction protein ZO-1 by substance P and IGF-1 in A431 cells

Cell Biochemistry and Function — Mon, 27 Jul 2009 23:00:00 +0100

The formation of a barrier by tight junctions is important in epithelia of various tissues. Substance P (SP) and insulin-like growth factor (IGF)-1 synergistically promote barrier function in the corneal epithelium. We have now examined the effects of SP and IGF-1 on expression of the tight-junction protein zonula occludens (ZO)-1 in A431 human epidermoid carcinoma cells. Reverse transcription-polymerase chain reaction (RT-PCR) and immunoblot analyses revealed that SP and IGF-1 increased the amounts of ZO-1 mRNA and protein in these cells in a concentration-dependent manner, with neither SP nor IGF-1 alone having such an effect. The SP- and IGF-1-induced up-regulation of ZO-1 was accompanied by phosphorylation of extracellular signal-regulated kinase (ERK), and both of these effects were b...

Overexpression of DPAGT1 Leads to Aberrant N-Glycosylation of E-Cadherin and Cellular Discohesion in Oral Cancer

Cancer Research — Tue, 14 Jul 2009 23:00:00 +0100

Cancer cells are frequently characterized by aberrant increases in protein N-glycosylation and by disruption of E-cadherin–mediated adherens junctions. The relationship between altered N-glycosylation and loss of E-cadherin adhesion in cancer, however, remains unclear. Previously, we reported that complex N-glycans on the extracellular domains of E-cadherin inhibited the formation of mature adherens junctions. Here, we examined whether dysregulated N-glycosylation was one of the underlying causes for cellular discohesion in oral cancer. We show that dense cultures of human salivary epidermoid carcinoma A253 cells exhibited elevated expression of DPAGT1, the gene that initiates protein N-glycosylation. Overexpression of DPAGT1 correlated with the production of E-cadherin–bearing...

Pyripyropenes, Fungal Sesquiterpenes Conjugated with alpha-Pyrone and Pyridine Moieties, Exhibits Anti-angiogenic Activity against Human Umbilical Vein Endothelial Cells.

Biological and Pharmaceutical Bulletin — Tue, 30 Jun 2009 23:00:00 +0100

Authors: Hayashi A, Arai M, Fujita M, Kobayashi M In the course of our search for anti-angiogenic substances, pyripyropenes A (1), B (2), and D (3) were re-discovered as selective anti-proliferative substances against human umbilical vein endothelial cells (HUVECs) from a marine-derived fungus of Aspergillus sp. Pyripyropenes showed potent anti-proliferative activity against HUVECs with IC(50) values of the range of 0.1-1.8 muM, which were cytostatic at 0.05 to 20 muM. The selective index was more than 55-fold in comparison with those of several tumor cell lines. Compound 1 inhibited vascular endothelial growth factor (VEGF)-induced migration and tubular formation of HUVECs, while 1 showed no effect on the VEGF-induced phosphorylations of extracellular signal-regulated kinase (ERK)1/2,...

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Inhibition of 12-LOX and COX-2 reduces the proliferation of human epidermoid carcinoma cells (A431) by modulating the ERK and PI3K-Akt signalling pathways

Experimental Dermatology — Tue, 23 Jun 2009 23:00:00 +0100

Please cite this paper as: Inhibition of 12-LOX and COX-2 reduces the proliferation of human epidermoid carcinoma cells (A431) by modulating the ERK and PI3K-Akt signalling pathways. Experimental Dermatology 2009.Abstract: Eicosanoids, the oxygenated metabolites of arachidonic acid (AA), mediate a variety of human diseases, such as cancer, inflammation and arthritis. To evaluate the role of eicosanoids in epidermoid carcinoma, the expression of AA metabolizing enzymes, such as lipoxygenases (LOXs) and cyclooxygenases (COXs), was analysed in a human epidermoid carcinoma cell line (A431). These studies revealed overexpression of 12-R-LOX and COX-2 in A431 cells. Baicalein (a 12-LOX inhibitor) and celecoxib (a COX-2 inhibitor) significantly reduced thymidine incorporation, whereas 12-(R)-HETE...

Metastatic Liposarcoma to the Parotid

Journal of Oncology — Tue, 09 Jun 2009 15:11:50 +0100

We report an exceptional case of parotid metastasis of myxoid liposarcoma in a 42-year-old man and insist on the worse prognosis of this entity. (Source: Journal of Oncology)

A dramatic case of genital radionecrosis

International Urology and Nephrology — Sun, 31 May 2009 08:22:43 +0100

Abstract Background Epidermoid carcinoma of the penis presents in 1% of malignant tumors in men. Penis cancer treatment has traditionally been total or partial amputation. There are authors who consider radiation to be part of penis cancer treatment in select cases—especially the use of brachytherapy and external radiation. Clinical case The case of a 48-year-old male who in 2004 presented with an ulcerated lesion of the penis that was left unattended to is presented. Two years later, he sought medical attention and physical examination revealed an ulcerated penile lesion with poorly defined edges and limited to the glans. Diagnosis The lesion was biopsied and the histopathological study reported Epidermoid Carcinoma. After radiological...

Anal Squamous Cell Carcinoma in the HIV-Positive Patient

Clinics in Colon and Rectal Surgery — Thu, 28 May 2009 08:29:49 +0100

This article describes the disease process and current treatment options.[...]© Thieme Medical PublishersGet connected:Table of contents | Abstract | Full text (Source: Clinics in Colon and Rectal Surgery)

Breast cancer resistant protein (BCRP) is a molecular determinant of the outcome of photodynamic therapy (PDT) for centrally located early lung cancer

Lung Cancer — Mon, 25 May 2009 00:00:00 +0100

Abstract: The ATP-binding cassette (ABC) transporter protein, BCRP (breast cancer resistance protein)/ABCG2 pumps out some types of photosensitizers used in photodynamic therapy (PDT) and causes resistance to the antitumor effect of PDT. The purpose of this study was to investigate the association between the expression of BCRP and the efficacy of PDT using Photofrin, or the second-generation photosensitizer, NPe6, for centrally located early lung cancers.Using human epidermoid carcinoma cells, A431 cells and the BCRP-overexpressing A431/BCRP cells, we examined the effects of BCRP expression on the effect of PDT by cell viability assay in vitro, and investigated the expression of BCRP by immunohistochemical analysis in 81 tumor samples obtained from patients with centrally located early lu...

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Malignant tumours development in peri-implant tissues in oncologic and non-oncologic patients: a report of two cases

International Journal of Oral and Maxillofacial Surgery — Fri, 01 May 2009 04:00:00 +0100

We report two cases of squamous cell carcinoma arising in peri-implant tissues after dental implant placement. A 35-year-old woman was referred to us one month after prosthetic rehabilitation following insertion of an implant in the area 36 four months earlier. She presented with a peri-implant tissue lesion with appearance of inflammatory tissue. Its biopsy was reported as infiltrate epidermoid carcinoma. Second case is a 75-year-old male with resection of epidermoid carcinoma affecting the lower left gingiva and mandible and reconstruction with iliac crest free flap fourteen years ago. Further fixed rehabilitation with osseo-integrated implants was carried out after reconstruction. Second primary malignancy in the tissue around one of the implant was developed. In both cases evolution wa...

A new acyclic diterpene acid and bioactive compounds from Knema glauca.

Archives of Pharmacal Research — Thu, 30 Apr 2009 23:00:00 +0100

Authors: Rangkaew N, Suttisri R, Moriyasu M, Kawanishi K Investigation of the chemical constituents of the fruits of Knema glauca (Myristicaceae) yielded a new acyclic diterpene acid, named glaucaic acid 4, together with four acylphenols, including 1-(2,6-dihydroxyphenyl) tetradecan-1-one 1, malabaricone A 6, dodecanoylphloroglucinol 7 and 1-(2,4,6-trihydroxyphenyl)-9-phenylnonan-1-one 8, two lignans sesamin 2 and asarinin 3, and a flavan, myristinin D 5. In addition, myristinin A 9 and (+/-)-7,4'-dihydroxy-3'-methoxyflavan 10 were isolated from its leaves and stems, respectively. When tested against small-cell lung cancer (NCI-H187), epidermoid carcinoma (KB) and breast cancer (BC) cell lines, compounds 1, 6-8 and 10 displayed weak to moderate cytotoxicity. The acylphenols 6-8 display...

Bee Propolis Stops Tumors from Neurofibromatosis and Cancer

NaturalNews.com — Wed, 29 Apr 2009 07:00:00 +0100

(NaturalNews) Honeybees are some of the most amazing creatures ever created by nature. They play a vital role in much of the food supply through pollination, and they provide people with a healthy natural alternative to sugar. Some of the most interesting aspects of bees are the substances they make to use themselves. Propolis is a natural resin found in young tree buds. Bees collect it for use as a glue to seal their homes. Propolis is an exceedingly complex product that contains substances that prevent and treat diseases ranging from cancer to the common cold. Recent research has documented the ability of propolis to suppress the growth of human tumors from neurofibromatosis and cancer.Compound from propolis halts tumor process in clinical trialsNeurofibromatosis is a genetic disorder th...

[Carcinoma of the lips.]

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Tue, 07 Apr 2009 23:00:00 +0100

Authors: Ben Slama L Epidermoid carcinoma, that is, squamous cell carcinoma of the skin, is the most common malignant tumor of the lips. It occurs especially in men. Its primary causes are sun exposure, smoking, and chronic irritation. Leukoplakia is the most frequent precancerous lesion. Epidermoid carcinoma may appear clinically as a scaly erosion or an ulceration. Standard treatment is surgical excision with reconstruction. PMID: 19361830 [PubMed - as supplied by publisher] (Source: Revue de Stomatologie et de Chirurgie Maxillo-Faciale)

Two New Sesquiterpenoids from Solanum lyratum with Cytotoxic Activities.

Chemical and Pharmaceutical Bulletin — Wed, 01 Apr 2009 04:00:00 +0100

Authors: Ren Y, Shen L, Zhang DW, Dai SJ Two new sesquiterpenoids, lyratol C (1) and lyratol D (2), together with two known sesquiterpenoids, dehydrovomifoliol (3) and blumenol A (4), were isolated from the whole plant of Solanum lyratum. Their structures were established by spectroscopic analyses. In vitro, the four compounds showed significant cytotoxic activities against three human cancer cell lines, namely, HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells, and gave IC(50) values in the range 3.7-8.1 muM. PMID: 19336938 [PubMed - in process] (Source: Chemical and Pharmaceutical Bulletin)

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Ganoderma tsugae extract inhibits expression of epidermal growth factor receptor and angiogenesis in human epidermoid carcinoma cells: In vitro and in vivo.

Cancer Letters — Sat, 28 Mar 2009 04:00:00 +0100

We examined the anti-angiogenic effects of Ganoderma tsugae methanol extract (GTME) on human epidermoid carcinoma A-431 cells. Our data indicate that GTME inhibits the expression of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) in vitro and in vivo, and also inhibits the capillary tube formation of human umbilical vein endothelial cells (HUVECs). We also show that the suppression of VEGF expression by GTME can be restored by treatment with EGF. These results suggest that GTME inhibits VEGF expression via the suppression of EGFR expression, resulting in the downregulation of VEGF secretion from epidermoid carcinoma A-431 cells. These findings reveal a novel role for G. tsugae in inhibiting EGFR and VEGF expression, which are important for tumor angiog...

Molecular Imaging and Quantitative Measurement of Epidermal Growth Factor Receptor Expression in Live Cancer Cells Using Immunolabeled Gold Nanoparticles

American Journal of Roentgenology — Thu, 19 Mar 2009 04:00:00 +0100

CONCLUSION. Immunolabeled nanoparticles can quantify receptor expression with performance comparable to fluorescence markers and show promise to better characterize receptor expression via their refractive index sensitivity. (Source: American Journal of Roentgenology)

Identification and characterization of a novel CXC chemokine in xenograft tumor induced by mas-overexpressing cells

International Journal of Cancer — Thu, 19 Mar 2009 00:00:00 +0100

Overexpressions of G protein-coupled receptor (GPCR) with elevated downstream signaling events have been reported in various tumors. However, the cellular mechanism that GPCR overexpression leads to tumor formation is largely unknown. The orphan GPCR mas was originally isolated from a human epidermoid carcinoma. In vivo studies of mas-overexpressing cells suggested that xenograft tumor formation was positively correlated with the levels of mas expression. Histochemical analysis indicated that xenograft tumor consisted of mas-transfected and stromal cells. Biochemical analyses revealed that cells overexpressing mas exhibited significantly increased anchorage-independent growth, whereas there was no significant difference in cell proliferation in comparison with empty vector-transfected cont...

A rare case of invasive vaginal carcinoma associated with vaginal prolapse

Archives of Gynecology and Obstetrics — Thu, 05 Mar 2009 14:59:36 +0100

Conclusions Careful preoperative histological evaluation of cervix in cases of uterine prolapse and postoperative cytologic and colposcopic follow-up of the vagina after hysterectomies are important because possible association with cervical carcinoma and occurrence of late vaginal cancer. Surgery and radiotherapy were effectively combined in this case that remains with no recurrence after 2 years follow-up. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s00404-009-1016-1Authors Thales Paulo Batista, University of Pernambuco Centro de Oncologia, Hospital Universitário Oswaldo Cruz Recife PE BrazilJosé Alves Morais, University of Pernambuco Centro de Oncologia, Hospital Universitário Oswaldo Cruz Recife PE BrazilTarcísio José Cysneiros Costa Rei...

Radiotherapy for Epidermoid Carcinoma of the Anus: Thirty Years' Experience

International Journal of Radiation Oncology * Biology * Physics — Mon, 02 Mar 2009 00:00:00 +0100

Conclusion: Our results have confirmed that CRT is an effective approach. Patients with human immunodeficiency virus can be treated with CRT. Tumor mobility significantly predicts the outcome; the implications for management are discussed. We also discuss the treatment planning implications of the late morbidity findings. The substantial incidence of additional malignancies underscores the importance of full oncologic screening during follow-up. (Source: International Journal of Radiation Oncology * Biology * Physics)

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Sarcophine-diol, a Chemopreventive Agent of Skin Cancer, Inhibits Cell Growth and Induces Apoptosis through Extrinsic Pathway in Human Epidermoid Carcinoma A431 Cells.

Translational Oncology — Sun, 01 Mar 2009 05:00:00 +0100

Authors: Zhang X, Bommareddy A, Chen W, Khalifa S, Kaushik RS, Fahmy H, Dwivedi C Sarcophine-diol (SD), a structural modifications of sarcophine, has shown chemopreventive effects on 7,12-dimethylbenz(a)anthracene-initiated and 12-O-tetradecanoylphorbol-13-acetate-promoted skin tumor developments in mice. Tumorigenesis is associated with uncontrolled cell growth and loss of apoptosis. In the present study, the effects of SD on cell growth and apoptosis in human epidermoid carcinoma A431 cells were determined to assess whether SD could inhibit cell growth and/or induce apoptosis, thus elucidating possible mechanism of action. MTT assay was used for cell viability; bromodeoxyuridine incorporation assay was used for cell proliferation; fluorescence-activated cell sorting analysis of annex...

[Tumoral pathology of salivary glands. Our experience]

Acta Otorrinolaringologica Espanola — Sun, 01 Mar 2009 00:00:00 +0100

CONCLUSIONS: We have found two main differences with previously reported studies: the elevated mean age of patients with malignant tumours and, probably as a result of this advanced age, the high incidence of squamous cell carcinoma due to metastatic dissemination of skin cancers. PMID: 19401079 [PubMed - in process] (Source: Acta Otorrinolaringologica Espanola)

[Adult Schönlein-Henoch purpura associated with epidermoid carcinoma of the lung.]

Nephrologie & Therapeutique — Thu, 12 Feb 2009 05:00:00 +0100

CONCLUSION: The neoplasm could be responsible of the development of the Schönlein-Henoch purpura. The discovery of this systemic vasculitis in an elderly patient should warrant a deep screening for an occult neoplasm. PMID: 19217841 [PubMed - as supplied by publisher] (Source: Nephrologie & Therapeutique)

[Isolated mandibular B-cell lymphoma revealed by inferior alveolar nerve anesthesia.]

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Mon, 02 Feb 2009 05:00:00 +0100

DISCUSSION: Our observation is unusual in its clinical presentation. Mandibular lymphomas most often present as a painless swelling, sometimes ulcerated in the mouth. They are very rarely diagnosed after an isolated hypoesthesia of V3. Lymphomas are the second most frequent head and neck lymphomas after epidermoid carcinomas, but the frequency seems to be increasing. In almost all the cases, they present as B-cell tumours of the DLBCL subtype in the WHO classification. Mandibular localizations account for only 0.6% of the cases. They are often misdiagnosed as a dental problem. The complete remission rate after chemotherapy ranges from 60 to 80% at one year. Nevertheless, the prognosis remains bad with a survival rate of only 50% at five years. PMID: 19193387 [PubMed - as supplied by pu...

Antiproliferative activity of Hungarian Asteraceae species against human cancer cell lines. Part II

Phytotherapy Research — Fri, 23 Jan 2009 05:00:00 +0100

The antiproliferative activities of aqueous and organic extracts prepared from 26 Hungarian species of the tribes Cynereae and Lactuceae (Asteraceae) were tested in vitro against HeLa (cervix epithelial adenocarcinoma), A431 (skin epidermoid carcinoma) and MCF7 (breast epithelial adenocarcinoma) cells by using the MTT assay. Of the tested 200 extracts of different plant parts obtained with n-hexane, chloroform, 50% methanol and water, 16 extracts displayed noteworthy cell growth inhibitory activity (>50% inhibition at a concentration of 10 µg/mL). The IC50 values of these extracts were determined, and their direct cytotoxic effects were measured. High differences between the antiproliferative and cytotoxic activities, demonstrating a real cell proliferation inhibitory activity rather than...

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The histopathological typing of 48 cases of ESO-phageal adenocarcinoma and its tissue origin

Chinese Journal of Cancer Research — Sat, 03 Jan 2009 06:42:00 +0100

Abstract Of 2487 cases of esophageal carcinoma pathologically confirmed after resection in 1978–1988 in Shantou, adenocarcinoma accounted for 1.93% (48 cases). These were divided into 6 types; Ordinary well - differentiated adenocarcinoma, 10 cases; Ordinary poorly - differentiated adenocarcinoma, 11 cases; Adeno - squamous carcinoma, 18 cases; Muco - epidermoid carcinoma, 4 cases; “Basal Cell - Like Carcinoma”, 4 cases; Cylindroma - Like carcinoma, 1 case. Mucohistochemistry and immuno-histochemistry studies revealed some differences among these types; And their prognosis also varied. The tissue origin of these tumors were discussed. Content Type Journal ArticleCategory Clinical ObservationsDOI 10.1007/BF03014411Authors Zhizhi Huang, Medical College of Shantou Un...

[Institutional report - Thoracic general] Sixty tracheal resections - single center experience

Interactive CardioVascular and Thoracic Surgery — Fri, 02 Jan 2009 05:00:00 +0100

This study evaluates the clinical outcome following surgery of our patients for the last seven years. Between 2001 and 2008 we performed tracheal resections in 60 patients. There were 46 cases of postintubation stenosis and 14 tumors. The range of resected rings was 1–8. The maximal resection length performed in our series (4 cm) was achieved using only basic releasing maneuvers such as anterior dissection of the trachea and cervical flexion. Emergency tracheal resection with no complications was performed in 12 patients who presented with severe dyspnea due to very tight stenosis. One patient died during the surgical intervention from a stroke. There were two postoperative deaths, both in patients with tracheo-esophageal fistula. As major complications we mention one patient wi...

Effects of Panduratin A Isolated from Kaempferia pandurata ROXB. on the Expression of Matrix Metalloproteinase-9 by Porphyromonas gingivalis Supernatant-Induced KB Cells.

Biological and Pharmaceutical Bulletin — Thu, 01 Jan 2009 05:00:00 +0100

In this study, panduratin A, a natural bioactive compound isolated from Kaempferia pandurata ROXB., was used to test its in vitro inhibitory activity against MMP-9 secretion from Porphyromonas gingivalis supernatant-induced human oral epidermoid carcinoma KB cells. Gelatin zymography, Western blot and RT-PCR analyses were performed to evaluate MMP-9 expression. The gelatin zymograms revealed that the main gelatinase secreted by P. gingivalis supernatant-induced KB cells migrated at 92 kDa, representing MMP-9. MMP-9 protein and mRNA levels were significantly decreased after panduratin A treatment (p<0.05). In contrast, panduratin A had no effect on tissue inhibitor of metalloproteinases (TIMP)-1 and TIMP-2 mRNA. Panduratin A also suppressed urokinase type plasminogen activator (uPA) mRNA...

Metastatic Liposarcoma to the Parotid

Journal of Oncology — Mon, 15 Dec 2008 12:51:54 +0100

We report an exceptional case of parotid metastasis of myxoid liposarcoma in a 42-year-old man and insist on the worse prognosis of this entity. (Source: Journal of Oncology)

Discovering novel 3-nitroquinolines as a new class of anticancer agents1

Acta Pharmacologica Sinica — Sun, 14 Dec 2008 05:00:00 +0100

Conclusion: This study was the first to identify new structural types of antiproliferative agents against the EGFR-overexpressing tumor cell lines by the incorporation of the nitro group at the 3-position of the quinoline core structure, providing promising new templates for the further development of anticancer agents. (Source: Acta Pharmacologica Sinica)

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Photodynamie activity on human larynx epidermoid carcinoma cell line, HEp-2

Medical Oncology — Fri, 05 Dec 2008 09:17:54 +0100

Content Type Journal ArticleCategory Research ReportsDOI 10.1007/BF02999864Authors S. Ganesan, Anna University Department of Physics 600 025 Madras IndiaV. Masitamani, Anna University Department of Physics 600 025 Madras India Journal Medical OncologyOnline ISSN 1559-131XPrint ISSN 1357-0560 Journal Volume Volume 11 Journal Issue Volume 11, Numbers 3-4 / September, 1994 (Source: Medical Oncology)

Radiation-induced carcinoma of the rectum

Diseases of the Colon & Rectum — Fri, 28 Nov 2008 14:08:53 +0100

We present a case of carcinoma of the rectum arising as two separate primaries 46 years after irradiation for an epidermoid carcinoma of the cervix. There were many pathologic changes related to the radiation therapy. Content Type Journal ArticleDOI 10.1007/BF02989000Authors Antonio Martins, Memorial Sloan-Kettering Cancer Center 1275 York Avenue 10021 New York New YorkStephen S. Sternberg, Memorial Sloan-Kettering Cancer Center 1275 York Avenue 10021 New York New YorkFadi F. Attiyeh, Memorial Sloan-Kettering Cancer Center 1275 York Avenue 10021 New York New York Journal Diseases of the Colon & RectumOnline ISSN 1530-0358Print ISSN 0012-3706 Journal Volume Volume 23 Journal Issue Volume 23, Number 8 / November, 1980 (Source: Diseases of the Colon & Rectum)

Discovering novel 3-nitroquinolines as a new class of anticancer agents.

Acta Pharmacologica Sinica — Thu, 27 Nov 2008 07:34:55 +0100

Enediyne lidamycin enhances the effect of epidermal growth factor receptor tyrosine kinase inhibitor, gefitinib, in epidermoid carcinoma A431 cells and lung carcinoma H460 cells.

Anti-Cancer Drugs — Fri, 21 Nov 2008 08:13:58 +0100

Page: 41DOI: 10.1097/CAD.0b013e328318292cAuthors: Liu, Hong; Li, Liang; Li, Xing-Qi; Liu, Xiu-Jun; Zhen, Yong-Su (Source: Anti-Cancer Drugs)

[Tumors of the oral cavity: the usefulness of dynamic CT.]

Radiologia — Sat, 01 Nov 2008 04:00:00 +0100

CONCLUSIONS: The habitual contact between the mucosal surfaces of the oral cavity makes some clinically obvious lesions difficult to identify at CT. The interposition of air acts as a natural contrast, separating the gums from the mucosa of the buccomaseteric region; PCCT provides relevant information and should be included in the imaging workup in patients with tumors of the oral cavity. PMID: 19100214 [PubMed - in process] (Source: Radiologia)

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[Tumors of the oral cavity: the usefulness of dynamic CT]

Radiologia — Sat, 01 Nov 2008 04:00:00 +0100

Metachronous bilateral mucoepidermoid carcinoma of the parotid gland

Indian Journal of Otolaryngology and Head & Neck Surgery — Wed, 22 Oct 2008 06:34:00 +0100

We report a very rare case of bilateral muco-epidermoid carcinoma of the parotid gland that underwent bilateral parotidectomy with neck dissections and radiotherapy. This case has done well for three years and suggests that metachronous bilateral mucoepidermoid carcinoma of the parotid gland, if treated as per the merits of each side, has a reasonable survival. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s12070-008-0086-xAuthors John C. Muthusami, Christian Medical College Hospital Vellore 632 004 Tamil Nadu IndiaPranay Gaikwad, Christian Medical College Hospital Vellore 632 004 Tamil Nadu IndiaJohn P. Raj, Christian Medical College Hospital Vellore 632 004 Tamil Nadu IndiaDeepak T. Abraham, Christian Medical College Hospital Vellore 632 004 Tamil Nadu IndiaMeera Tho...

G-protein-coupled receptor-associated A-kinase anchoring proteins AKAP5 and AKAP12: Differential trafficking and distribution.

Cellular Signalling — Fri, 10 Oct 2008 04:00:00 +0100

Authors: Chen MH, Malbon CC A-kinase Anchoring Proteins (AKAPs) define an expanding group of scaffold proteins that display a signature binding site for the RI/RII subunit of protein kinase A. AKAP5 and AKAP12 are multivalent (with respect to protein kinases and phosphatases) and display the ability to associate with the prototypic member of G protein-coupled receptors, the beta(2)-adrenergic receptor. We probed the relative abundance, subcellular distribution and localization of AKAP5 and AKAP12 in human embryonic kidney HEK293 and epidermoid carcinoma A431 cells. HEK293 cells are relatively rich in AKAP5 (found mostly in association with the cell membrane); whereas A431 cells are rich in AKAP12 (found distributed both in the cytoplasm and in association with the cell membrane). In bi...

[Scintigraphy in staging upper aerodigestive tract epidermoid carcinomas.]

Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale — Thu, 11 Sep 2008 04:00:00 +0100

CONCLUSION: The incidence of bone metastases in the initial extension assessment was low; consequently, they are not sought systematically. However, their presence radically changes the prognosis and the therapeutic management, raising the question of screening. The technetium-99m bone scintigraphy has limits, with many false-positive resulting the need for additional investigations. Defining the risk factors for bone metastases would improve screening. Two questions remain: what factors are involved? The bone pain and the hypercalcemia must be analyzed with a larger number of cases, but they seem to be nonspecific. The node involvement stage could be a more reliable parameter, in particular in cases of jugular vein invasion; what method should be used? In the future, the PET scan could be...

[Double simultaneous fingertip metastasis of an epidermoid carcinoma. A case report.]

Chirurgie de la Main — Thu, 04 Sep 2008 04:00:00 +0100

Authors: Ottomani S, Levadoux M, Cathelineau O, Nguyen M, Monchal T Fingertip metastases are rare. The authors report the case of a patient with an epidermoid carcinoma of the larynx who presented with two separate finger pulp metastases in each hand. According to the literature this seems a very unusual occurrence. This diagnosis must be considered in the differential diagnosis of every suspicious fingertip lesion. PMID: 18818116 [PubMed - as supplied by publisher] (Source: Chirurgie de la Main)

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Reversal effect of resveratrol on multidrug resistance in KBv200 cell line.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie — Thu, 28 Aug 2008 04:00:00 +0100

Authors: Quan F, Pan C, Ma Q, Zhang S, Yan L A multidrug-resistant clone of human oral epidermoid carcinoma KB cells was isolated by stepwise selection on exposure to increasing doses of vincristine. The final clone, KBv200, obtained after ethylmethane sulfonate mutagenesis showed 156-fold higher resistance to vincristine than KB cells. The cells were also cross-resistant to paclitaxel and adriamycin. The aim of this study was to explore the reversal effect and potential mechanism of resveratrol on KBv200 cells. MTT assay was used to investigate the reversal index of resveratrol to vincristine, adriamycin and paclitaxel. Cell apoptosis was measured by flow cytometry. RT-PCR and western blot were used to detect mRNA and protein expression of multidrug-resistant gene MDR1 and apoptosis-s...

[Is it Necessary to Perform Neck Dissections as a Staged Procedure in Laser Supraglottic Laryngectomy?]

Acta Otorrinolaringologica Espanola — Fri, 01 Aug 2008 04:00:00 +0100

CONCLUSIONS: It is a safe procedure to perform neck dissections at the same time as the supraglottic laryngectomy. We did not find any increase in the complications rate and the hospital stay was shorter. PMID: 18817717 [PubMed - in process] (Source: Acta Otorrinolaringologica Espanola)

Synthesis and in vitro antitumor activities of novel 4-anilinoquinazoline derivatives.

European Journal of Medicinal Chemistry — Sat, 26 Jul 2008 04:00:00 +0100

Authors: Chandregowda V, Kush AK, Chandrasekara Reddy G A series of 6, 7-dialkoxy-4-anilinoquinazolines were designed, synthesized by substituting different heterocycles on 6-position and a variety of anilines on 4-position of the quinazoline. These novel quinazoline compounds were screened for their cytotoxic effect on epidermal growth factor receptor overexpressing skin epidermoid carcinoma cell line (A431), by using nonoverexpressing tumor cells as negative control (breast adeno carcinoma cell line MCF-7). 2-Butyl-4-chloro-1-{3-[7-methoxy-4-(3-(trifluoromethyl)phenylamino)quinazolin-6-yloxy]-propyl}-1H-imidazole-5-carboxaldehyde (30) and 2-butyl-4-chloro-1-{3-[4-(3-iodophenyl amino)-7-methoxyquinazolin-6-yloxy]propyl}-1H-imidazole-5-carboxaldehyde (33) were found to be more potent a...

Epidermoid carcinoma involving the parotid gland and orbital region: Case report of a rare entity.

Ear, Nose and Throat Journal — Tue, 01 Jul 2008 04:00:00 +0100

Authors: Aslan S, Yavuz H, Altan-Yaycioglu R, Bolat F, Ozgirgin N Epidermoid carcinoma (also known as squamous cell carcinoma) of the parotid gland is more prevalent among the elderly, as are other head and neck tumors. In most cases it is observed either as a nodal metastasis of a primary tumor that drains into the parotid area or as a direct extension of a tumor that has originated in the upper dermis. Primary epidermoid carcinomas arising directly from the parotid gland are extremely rare. Similarly, secondary invasion of the orbital region by a neighboring tumor is more common than primary epidermoid carcinoma of the orbit, which is also rare. In this article, we discuss the diagnosis and treatment of a rare case of epidermoid carcinoma simultaneously invading the parotid gland and...

BCH, an Inhibitor of System L Amino Acid Transporters, Induces Apoptosis in Cancer Cells.

Biological and Pharmaceutical Bulletin — Wed, 04 Jun 2008 14:50:53 +0100

Conclusion: These results suggest that the inhibition of LAT1 activity by BCH leads to apoptotic cancer cell death by inducing intracellular depletion of neutral amino acids necessary for cancer cell growth. PMID: 18520037 [PubMed - as supplied by publisher] (Source: Biological and Pharmaceutical Bulletin)

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Two new neo-clerodane diterpenoids from Scutellaria barbata.

Journal of Integrative Plant Biology — Sun, 01 Jun 2008 04:00:00 +0100

Authors: Dai SJ, Shen L, Ren Y Two new neo-clerodane diterpenoids, 6,7-dibenzoyloxybarbatin C (1, named barbatin D) and 6-(2-acetoxy-3-methylbutanoloxy)-7-(2-carbonyl-3-methylbutanoyloxy) barbatin C (2, named barbatin E) were isolated from the whole plant of Scutellaria barbata D. Don. Their structures were elucidated by spectroscopic methods including extensive 1D and 2D NMR analyses. In vitro, compounds 1-2 showed cytotoxic activities against three human cancer lines, namely, HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells, and with IC(50) values in the range of 3.5-6.7 microM. PMID: 18713410 [PubMed - in process] (Source: Journal of Integrative Plant Biology)

Incidental finding of an intrauterine device in the sigmoid colon.

The European Journal of Contraception and Reproductive Health Care — Sun, 01 Jun 2008 04:00:00 +0100

CONCLUSION: Asymptomatic migration of IUD to the sigmoid colon lumen can occur. Except when the patient's condition does not allow it, as in the case reported, removal of the IUD is indicated because of the risk of fistula formation and colon perforation with a high ensuing morbidity. This case report highlights the need for follow-up of patients wearing an IUD. PMID: 18465486 [PubMed - indexed for MEDLINE] (Source: The European Journal of Contraception and Reproductive Health Care)

Cytotoxic activities of trichothecenes isolated from an endophytic fungus belonging to order hypocreales.

Archives of Pharmacal Research — Thu, 01 May 2008 04:00:00 +0100

Authors: Chinworrungsee M, Wiyakrutta S, Sriubolmas N, Chuailua P, Suksamrarn A Bioassay-guided fractionation of the extract of the endophytic fungus KLAR 5 belonging to order Hypocreales, which was isolated from the twig of Knema laurina (Blume) Warb., resulted in the isolation of brefeldin A (1), 8-deoxy-trichothecin (2), trichothecolone (3), 7alpha-hydroxytrichodermol (4), and 7alpha-hydroxyscirpene (5). Compound 5 was isolated from natural source for the first time. Compound 1 was very highly active against human epidermoid carcinoma of the mouth, human breast cancer (BC-1), and human small cell lung cancer (NCI-H187) cells whereas compounds 2 and 4 were selectively active against BC-1 and NCI-H187 cells. Compounds 3 and 5 were moderately active against these three cancer cell line...

[Partial pharyngolaryngectomies for treatment of pharyngolaryngeal cancer.]

Cirugia y Cirujanos — Thu, 01 May 2008 04:00:00 +0100

Conclusions: Voice conservation surgery is an alternative to mutilation of the larynx in patients with locally advanced neoplasms of the larynx with a high risk of recurrence if treated with radiotherapy. PMID: 18647554 [PubMed - in process] (Source: Cirugia y Cirujanos)

[Occupational exposure to wood dust and nasal sinus cancer.]

Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale — Tue, 22 Apr 2008 04:00:00 +0100

CONCLUSION: Epidemiologic data, such as the increasing incidence, and clinical and professional data, such as the occupational exposure to wood dust, were in agreement with the French and European literature. It is still probably too early to appreciate the effectiveness of prevention, established in France since 1980, on nasal sinus tumor incidence. PMID: 18436189 [PubMed - as supplied by publisher] (Source: Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale)

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Soluble EMMPRIN (extra-cellular matrix metalloproteinase inducer) stimulates the migration of HEp-2 human laryngeal carcinoma cells, accompanied by increased MMP-2 production in fibroblasts.

Arch Histol Cytol — Tue, 01 Apr 2008 04:00:00 +0100

Authors: Hanata K, Yamaguchi N, Yoshikawa K, Mezaki Y, Miura M, Suzuki S, Senoo H, Ishikawa K The basement membrane functions as a barrier against the invasion of cancer cells. It is therefore important to investigate the mechanism of basement membrane degradation by matrix metalloproteinases (MMPs). Previously, cancer cells were long considered to be the major source of MMPs; however, current evidence indicates that most MMPs in cancer tissue are produced by stromal rather than cancer cells. A glycoprotein highly expressed on the cancer-cell membrane, EMMPRIN (extra-cellular matrix metalloproteinase inducer), exhibits the potential role of the MMP inductor in stromal cells. Depending on the cell type, EMMPRIN can stimulate the production of MMP-1, MMP-2, and MMP-3. We here report that...

[Breast metastasis from vulvar carcinoma: Case report and review of literature.]

Cancer radiotherapie — Fri, 14 Mar 2008 04:00:00 +0100

We report the case of a 42 year-old patient who underwent a treatment because of vulvar epidermoid carcinoma, right away metastatic at the level of the inguinal ganglia. The treatment consisted in a total vulvectomy with bilateral ganglial curretage, followed by external radiotherapy about the perineum and the inguinal ganglia. Three months after the end of her treatment, the patient presented with a nodula on the left outer breast with features of malignancy noticed by clinic and mammographic examination. The histologic study of the mammary biopsy showed epidermoid carcinoma of likely metastatic origin. A left Patey has been realized and confirmed the metastatic localization of epidermoid carcinoma with axillary ganglial metastasis (2N+/-7). Besides, this patient presented a right cervica...

[Mouth floor and mobile tongue epidermoid carcinomas thickness: Prognostic value.]

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Wed, 05 Mar 2008 05:00:00 +0100

This study suggests that tumor thickness should be taken in consideration in T1N0, T2N0 and T3N0 mouth floor and mobile tongue epidermoid carcinomas. In the future, the clinical evaluation of tumor thickness will help determine the therapeutic management. PMID: 18328517 [PubMed - as supplied by publisher] (Source: Revue de Stomatologie et de Chirurgie Maxillo-Faciale)

Esophagus - Esophageal Squamous Papilloma

The Digital Atlas of Video Education - Gastroenterology — Sun, 02 Mar 2008 08:03:19 +0100

In conclusion squamous cell papilloma is an uncommon benign esophageal tumor which must be removed in all patients because of concern regarding malignant potential. (Source: The Digital Atlas of Video Education - Gastroenterology)

[A novel retinoid CD437 induces apoptosis of human epidermoid carcinoma A431 cells.]

Journal of Southern Medical University — Sat, 01 Mar 2008 05:00:00 +0100

CONCLUSION: CD437 is more effective than ATRA in inhibiting the proliferation and inducing apoptosis in A431 cells and shows selective apoptosis-inducing effect against malignant keratinocytes, suggesting its potential in the prevention or treatment of cutaneous carcinoma. PMID: 18359678 [PubMed - in process] (Source: Journal of Southern Medical University)

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Safety of repeated administrations of ixabepilone given as a 3-hour infusion every other week in combination with irinotecan in patients with advanced malignancies.

European Journal of Cancer — Tue, 26 Feb 2008 05:00:00 +0100

In conclusion, toxicity including peripheral neuropathy was manageable at the recommended doses of 20 mg/m(2) ixabepilone combined with 180 mg/m(2) irinotecan on days 1 and 14 every 28 days. Promising antitumour activity was observed in patients with platinum-pretreated lung cancer. PMID: 18308561 [PubMed - as supplied by publisher] (Source: European Journal of Cancer)

Reconstruction of the Lower Lip: Bernard Technique and Its Variants

Dermatologic Surgery — Wed, 06 Feb 2008 15:56:27 +0100

Dermatologic Surgery, Volume 0, Issue 0, Page ???, OnlineEarly Articles. INTRODUCTION Surgical treatment of epidermoid carcinoma of the lower lip occasionally involves resection of a large part of this anatomic area. Several techniques are available for its repair, including Bernard's technique. Since its first description ... (Source: Dermatologic Surgery)

Molecular mechanism of black tea polyphenols induced apoptosis in human skin cancer cells: involvement of Bax translocation and mitochondria mediated death cascade

Carcinogenesis — Thu, 31 Jan 2008 05:00:00 +0100

This study investigated the induction of apoptosis in human skin cancer cells after treatment of TF and TR. We report that both TF and TR could exert inhibition of A431 (human epidermoid carcinoma) and A375 (human malignant melanoma) cell proliferation without adversely affecting normal human epidermal keratinocyte cells. Growth inhibition of A375 cells occurred through apoptosis, as evident from cell cycle arrest at G0/G1 phase, increase in early apoptotic cells, externalization of phosphatidylserine and DNA fragmentation. In our pursuit to dissect the molecular mechanism of TF- and TR-induced apoptosis in A375 cells, we investigated whether cell death is being mediated by mitochondria. In our system, Bax translocation to mitochondria persuaded depolarization of mitochondrial membrane pot...

Lymphatic mapping and sentinel lymph node biopsy in epidermoid carcinoma of the anal canal.

European Journal of Surgical Oncology — Fri, 04 Jan 2008 05:00:00 +0100

CONCLUSIONS: Inguinal sentinel node biopsy in anal cancer is efficient and could assist in the decision for inguinal radiation. The validity and safety of the proposed therapeutic algorithm has to be proven by a larger, prospective study. PMID: 18178364 [PubMed - as supplied by publisher] (Source: European Journal of Surgical Oncology)

Diverse TNFalpha-induced death pathways are enhanced by inhibition of NF-kappaB.

International Journal of Oncology — Sat, 01 Dec 2007 05:00:00 +0100

Authors: Katdare M, Efimova EV, Labay E, Khodarev NN, Darga TE, Garofalo M, Nakamura S, Kufe DW, Posner MC, Weichselbaum RR TNFalpha was initially described as inducing necrotic death in tumors in vivo, and more recently as a cytokine that mediates cytoprotection and inflammation. The anti-tumor effects of TNFalpha are poorly characterized because TNFalpha-induced death of human tumor cells has largely been studied in the presence of agents that block transcription or protein synthesis. Also, most reports in model cell systems describe apoptosis within relatively early time points as the principal mode of cell death induced by TNFalpha. We investigated the cytotoxic effects of 10 ng/ml TNFalpha on human tumor cells of different histological types without concomitant exposure to these i...

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[Systematic neck dissection in squamous cell carcinoma of the oral cavity]

Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale — Sat, 01 Dec 2007 05:00:00 +0100

CONCLUSION: The systematic cervical lymph node dissection remains the most effective means to obtain the neck control of squamous cell cancers of the oral cavity. It however was applied without therapeutic value for 45% of the patients of this series. The validation of the sentinel node concept as a method of cervical staging should make it possible to avoid this surgical procedure in more than one third of the cases. PMID: 17673159 [PubMed - indexed for MEDLINE] (Source: Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale)

Identification on membrane and characterization of phosphoproteins using an alkoxide-bridged dinuclear metal complex as a phosphate-binding tag molecule.

Journal of Biomolecular Techniques : JBT — Sat, 01 Dec 2007 05:00:00 +0100

Authors: Nakanishi T, Ando E, Furuta M, Kinoshita E, Kinoshita-Kikuta E, Koike T, Tsunasawa S, Nishimura O We have developed a method for on-membrane direct identification of phosphoproteins, which are detected by a phosphate-binding tag (Phos-tag) that has an affinity to phosphate groups with a chelated Zn2+ ion. This rapid profiling approach for phosphoproteins combines chemical inkjet technology for microdispensing of reagents onto a tiny region of target proteins with mass spectrometry for on-membrane digested peptides. Using this method, we analyzed human epidermoid carcinoma cell lysates of A-431 cells stimulated with epidermal growth factor, and identified six proteins with intense signals upon affinity staining with the phosphate-binding tag. It was already known that these pro...

Human papillomavirus in oral squamous cells carcinoma in a population of 75 Brazilian patients.

American Journal of Otolaryngology — Thu, 01 Nov 2007 04:00:00 +0100

CONCLUSION: The low frequency of detection of HPV DNA in oral epidermoid carcinomas suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors. PMID: 17980772 [PubMed - in process] (Source: American Journal of Otolaryngology)

Bioactive Capsular Polysaccharide from the Thermophilic Cyanophyte/Cyanobacterium Mastigocladus laminosus - Cytotoxic Properties

Planta Medica — Tue, 23 Oct 2007 01:24:56 +0100

Planta MedDOI: 10.1055/s-2007-990237AbstractThe capsular polysaccharide produced by the thermophilic blue green alga/cyanobacterium was tested for its cytotoxic activity against the A431 human epidermoid carcinoma cell line. This polysaccharide inhibited the proliferation of A431 cells in a dose-dependent manner with an IC value of 50 μg mL. In addition, this polysaccharide strongly inhibited A431 cell migration and invasion. Preliminary experiments showing that secretion of metalloproteinases MMP2 and MMP9 by A431 tumour cells was inhibited by this polysaccharide suggest that this mechanism of action could play a role in its anti-migration and anti-invasive properties. Acid hydrolysis of the polysaccharide produced specific oligosaccharides which conserved - at similar concentrations...

Bioactive ent-Clerodane Diterpenoids from Scutellaria barbata

Planta Medica — Tue, 23 Oct 2007 01:24:56 +0100

Planta Med 2007; 73: 1217-1220DOI: 10.1055/s-2007-990215AbstractFour new -clerodane diterpenoids were isolated from the whole plant of D. Don. (Labiatae). Their structures were elucidated by chemical methods and spectral analyses. , the four new compounds showed significant cytotoxic activities against three human cancer lines (HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells), and gave IC values in the range 3.1 - 7.2 M.[...]© Georg Thieme Verlag KG Stuttgart · New YorkGet connected:Table of contents | Abstract | Full text (Source: Planta Medica)

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Substituted titanocenes induce caspase-dependent apoptosis in human epidermoid carcinoma cells in vitro and exhibit antitumour activity in vivo

British Journal of Cancer AOP — Tue, 09 Oct 2007 04:00:00 +0100

J H Bannon, I Fichtner, A O'Neill, C Pampillon, N J Sweeney, K Strohfeldt, R W Watson, M Tacke & M M Mc Gee (Source: British Journal of Cancer AOP)

Prolonged survival in bronchogenic carcinoma complicated by superior vena cava obstruction

Lung — Fri, 05 Oct 2007 15:55:55 +0100

Abstract Prolonged survival in patients with bronchogenic carcinoma and superior vena cava syndrome is rare. One patient with this syndrome due to epidermoid carcinoma of the lung has survived 10 years following radiation therapy. Occasional patients with superior vena cava syndrome due to lung carcinoma may have long periods of disease-free survival. Therefore, curative radiation therapy is indicated for this condition. Content Type Journal ArticleDOI 10.1007/BF02713891Authors A. Schecter, Hospital at the Albert Einstein College of Medicine the Pulmonary Dept. 1825 Eastchester Road 10461 Bronx NY USAJ. Reichel, Hospital at the Albert Einstein College of Medicine the Pulmonary Dept. 1825 Eastchester Road 10461 Bronx NY USA Journal LungOnline ISSN 1432-1750Print IS...

[Degeneration of dermoid cysts: a case study of malignant transformation.]

Gynecologie, Obstetrique & Fertilite — Tue, 02 Oct 2007 04:00:00 +0100

We report the case of a 58-year-old woman showing abdominal pain associated with constipation episodes. The diagnosis of dermoid cyst was established upon ultrasonographic and tomodensitometric criteria. Its histopathological analysis confirmed the presence of a well differentiated epidermoid carcinoma. In line with the literature, our study highlights the importance of the age of the patient, the size of dermoid cyst and its growth rate as well as squamous cell carcinoma (SCC) antigen serum level. These date are collected in order to establish a correct diagnosis and provide an appropriate cure. When a cyst is discovered these data must pathological event, mostly observed in menopaused women. Certainty diagnosis is collected in order to establish a correct diagnosis and provide an appropr...

KGF promotes integrin {alpha}5 expression through CCAAT/enhancer-binding protein-beta

AJP: Cell Physiology — Tue, 11 Sep 2007 04:00:00 +0100

Keratinocyte growth factor (KGF) and 5ß1-integrin are not expressed in normal skin but they are both highly upregulated in the migrating epidermis during wound healing. Here we report that KGF increased 5 mRNA and protein levels in epidermoid carcinoma cells and stratified bioengineered epidermis. Interestingly, KGF increased integrin 5 in the basal as well as suprabasal cell epidermal layers. Promoter studies indicated that KGF-induced integrin 5 promoter activation was dependent on the C/EBP transcription factor binding site. Accordingly, KGF induced sustained phosphorylation of C/EBP-ß that was dependent on activation of ERK1/2. In addition, a dominant negative form of C/EBP-ß inhibited 5 promoter activity and blocking C/EBP-ß with siRNA diminished integrin 5 exp...

Immunonanoshells for targeted photothermal ablation in medulloblastoma and glioma: an in vitro evaluation using human cell lines

Journal of Neuro-Oncology — Thu, 06 Sep 2007 07:26:33 +0100

In this study, we evaluated the efficacy of immunonanoshells in vitro against both medulloblastoma and high-grade glioma cell lines. We used an antibody against HER2 to target gold-silica nanoshells to medulloblastoma cells, since HER2 is frequently overexpressed in medulloblastoma. We show that treatment with HER2-targeted nanoshells, but not non-targeted nanoshells, followed by exposure to laser light, can induce cell death in the HER2-overexpressing medulloblastoma cell line Daoy.2, as well as the parental Daoy cell line, which expresses HER2 at a moderate level, but not in dermal fibroblasts that do not express HER2. In an analogous set of experiments, we conjugated gold-silica nanoshells to an antibody against interleukin-13 receptor-alpha 2 (IL13Rα2), an antigen that is f...

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Evaluation of resveratrol and piceatannol cytotoxicity in macrophages, T cells, and skin cells.

Arhiv za Higijenu Rada i Toksikologiju — Sat, 01 Sep 2007 04:00:00 +0100

Authors: Radkar V, Hardej D, Lau-Cam C, Billack B The cytotoxicity of resveratrol and of piceatannol, a structural analog of resveratrol, was examined in cultured cells. Using a MTT-based assay, which measures the conversion of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) to a colored formazan product in living cells, resveratrol was found to inhibit the viability of transformed mouse macrophages, tumor-derived human T cells and human epidermoid carcinoma cells in a concentration-dependent manner, with the effect decreasing in the order: T cells (LC50 approximately 27 micromol L(-1), 24 h; approximately 9 micromol L(-1); 48 h)>macrophages (LC50 approximately 29 micromol L(-1), 24 h; 39 micromol L(-1), 48 h)>skin cells (LC50 approximately 91 micromol L(-1), ...

[Human recombinant erythropoietin-alpha increases the efficacy of irradiation in preclinical model]

Magyar Onkologia — Thu, 30 Aug 2007 13:07:44 +0100

Authors: Lövey J, Kenessey I, Rásó E, Dobos J, Vágó A, Kásler M, Futosi K, Döme B, Tímár J, Tóvári J According to recent data erythropoietin receptor (EPOR) is expressed not only by bone marrow erythroid progenitors but by endothelial- and cancer cells and it was suggested that erythropoietin (EPO) may affect their functions as well. We have analyzed the effects of recombinant human erythropoietin-alpha (rHuEPOalpha) on radiation sensitivity of EPOR+ human epidermoid carcinoma (A431) xenograft model. In vivo rHuEPOalpha treatment was started after tumor cell inoculation into SCID mice. 5 Gy irradiation was performed on day 14, the effect of which was measured on day 22. Hemoglobin level, tumor-associated microvessels and HIF-1alpha...

Massive intracardiac metastases secondary to squamous cell carcinoma located at the level of the penis.

International Journal of Cardiology — Thu, 16 Aug 2007 04:00:00 +0100

We present a single case of massive intracardiac metastases secondary to epidermoid carcinoma of the penis. This is an extremely rare entity which has scarcely been documented. PMID: 17707529 [PubMed - as supplied by publisher] (Source: International Journal of Cardiology)

Pseudoachalasia of the Cardia Secondary to Nongastrointestinal Neoplasia

Dysphagia — Wed, 15 Aug 2007 06:53:25 +0100

Abstract A minor proportion of patients with achalasia eventually have a neoplasm and, as a consequence, pseudoachalasia is diagnosed. A neoplasm may either involve gastrointestinal junction or present a paraneoplastic effect. Over the global diagnoses of achalasia issued in 5 years of experience in our motility unit, we have found 13% (3/23 cases) of pseudoachalasia (2–4% in previous series, probably due to the fact that the population assisted was mainly composed of elderly patients). The origin of the neoplasm was bladder, prostate and metastases from epidermoid carcinoma of vocal chord. Treatment of primary neoplasm, besides classical approach (with dilatation of botulinum injection) may help in the resolution of this clinical disorder. Content TypeJournal Art...

Antiproliferative activity of Hungarian Asteraceae species against human cancer cell lines. Part I

Phytotherapy Research — Fri, 27 Jul 2007 04:00:00 +0100

Aqueous and organic extracts of 25 selected species from four tribes of Hungarian Asteraceae were screened in vitro for antiproliferative activity against HeLa (cervix epithelial adenocarcinoma), A431 (skin epidermoid carcinoma) and MCF7 (breast epithelial adenocarcinoma) cells, using the MTT assay. Twenty five of the 228 tested extracts from different parts of the species of Astereae (6), Inuleae (3), Heliantheae (5) and Anthemideae (11) demonstrated a substantial antiproliferative effect (at least 50% inhibition of cell proliferation) at 10 µg/mL against one or more of the cell lines. Complete dose-response curves were generated and IC50 values were calculated for these active extracts, and their direct cytotoxic effects were determined. In summary, 11 of the tested 25 plants were found...

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EGF-induced apoptosis in A431 cells is dependent on STAT1, but not on STAT3.

European Journal of Cell Biology — Sat, 21 Jul 2007 04:00:00 +0100

Authors: Grudinkin PS, Zenin VV, Kropotov AV, Dorosh VN, Nikolsky NN EGF in high concentrations has a growth-inhibitory effect on human epidermoid carcinoma cells A431. The transcription factor STAT1 is the most probable candidate for mediating this effect. In the present study, we demonstrated a strong reduction of the expression level of STAT1 in EGF-resistant sub-clones of A431 cells. EGF resistance was reversed by introducing wild-type STAT1, but not its Y701F mutant. Moreover, blocking the activity of Src family kinases reduced tyrosine phosphorylation of STAT1 and STAT3 and protected A431 cells from the EGF-induced growth inhibition. To further elucidate roles of STATs in A431 cell growth and survival, clones of A431 cells expressing short hairpin RNA (shRNA) against STAT1 or STA...

Toxicity of Dimethylmonothioarsinic Acid toward Human Epidermoid Carcinoma A431 Cells

Chemical Research in Toxicology — Fri, 13 Jul 2007 04:00:00 +0100

Hua Naranmandura, Kenji Ibata, and Kazuo T. SuzukiWeb Release Date: Fri, 13 Jul 2007 00:00:00 EDT (Article) DOI: 10.1021/tx700103y (Source: Chemical Research in Toxicology)

Combretastatin-chalcone hybrids: synthesis and cytotoxicity.

Medicinal Chemistry — Sun, 01 Jul 2007 04:00:00 +0100

Authors: Nam NH, Byung-Zun A A series of all-trans-1-aryl-4-aryl-5-aryl-2,4-pentanediene-1-one (3), a hybridized form of chalcone and combretastatin, was synthesized and evaluated against a panel of cancer cell lines, including B16, murine melanoma; HCT116, colon cancer; A431, human epidermoid carcinoma; and human umbilical venous endothelial cells (HUVEC). Structure-activity relationships analysis of this series revealed that a 2,5-dihydroxyphenyl at position 1 of the 2,4-pentanediene-1-one was essential for cytotoxicity. all-trans-1-(2,5-Dihydroxyphenyl)-5-(4-methoxyphenyl)-4-(3,4,5-trimethoxyphenyl)-2,4-pentanediene-1-one (3a) was the most potent compound from this series. PMID: 17627575 [PubMed - indexed for MEDLINE] (Source: Medicinal Chemistry)

In Vitro cytotoxic activity of some Korean medicinal plants on human cancer cell lines: enhancement in cytotoxicity by heat processing

Phytotherapy Research — Fri, 29 Jun 2007 04:00:00 +0100

The goal of this study was to examine the in vitro antiproliferative activity of crude methanol extracts of three traditional Korean medicinal plants: Achyranthes fauriei, Epimedium koreanum Nakai and Scutellaria baicalensis before and after heat processing. The extracts were screened for antitumoral potential by means of an MTT assay on four human cancer cell lines: lung cancer cells (Lu1), colon cancer cells (Col2), oral epidermoid carcinomas (KB) and hormone-dependent prostate cancer cells (LNCaP). None of the extracts showed significant activity against any of the cancer cell lines. However, after treatment with steam, the processed Achyranthes fauriei extract exhibited a slight, but enhanced cytotoxic activity against all four of the cancer cell lines. The processed Scutellaria baical...

KGF promotes integrin alpha-5 expression through CCAAT/enhancer-binding protein-beta.

Am J Physiol Cell Ph... — Wed, 27 Jun 2007 04:00:00 +0100

Authors: Koria P, Andreadis ST Keratinocyte growth factor (KGF) and integrin alpha5-beta1 are not expressed in normal skin but they are both highly upregulated in the migrating epidermis during wound healing. Here we report that KGF increased alpha-5 mRNA and protein levels in epidermoid carcinoma cells and stratified bioengineered epidermis. Interestingly, KGF increased integrin alpha-5 in the basal as well as suprabasal cell epidermal layers. Promoter studies indicated that KGF-induced integrin alpha-5 promoter activation was dependent on the C/EBP transcription factor binding site. Accordingly, KGF induced sustained phosphorylation of C/EBP-beta that was dependent on activation of ERK1/2. In addition, a dominant negative form of C/EBP-beta inhibited alpha-5 promoter activity and blo...

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Optical biosensor differentiates signaling of endogenous PAR1 and PAR2 in A431 cells

BioMed Central — Fri, 22 Jun 2007 04:00:00 +0100

Conclusions: Optical biosensor provides an alternative readout for examining receptor activation under physiologically relevant conditions, and differentiates the signaling of endogenous PAR1 and PAR2 in A431. (Source: BioMed Central)

A brief statistical review of epidermoid carcinoma of the anal canal and rectum

Diseases of the Colon & Rectum — Sat, 05 May 2007 09:45:49 +0100

Summary A brief review of the terminology, incidence and treatment recommended for epidermoid carcinoma of the anal canal and rectum is presented. Statistical records of metastatic spread and five-year survival rates are also stated briefly. Content TypeJournal Article JournalDiseases of the Colon & RectumOnline ISSN 1530-0358Print ISSN 0012-3706 Journal VolumeVolume 8 Journal IssueVolume 8, Number 5 / September, 1965 (Source: Diseases of the Colon & Rectum)

Epidermoid carcinoma arising in a pilonidal sinus

Diseases of the Colon & Rectum — Sat, 05 May 2007 09:45:44 +0100

Conclusions The seventeenth case of epidermoid carcinoma arising in a pilonidal sinus is presented. Treatment and results of reported cases are reviewed. For the sake of completeness, four additional cases of unspecified types of pilonidal cancer are included. A review of all reported cases indicates that: 1) Distant metastasis occurs with about the same frequency as with epidermoid carcinoma arising in antecedent benign lesions elsewhere in the body; 2) Local treatment should consist of wide surgical removal. Irradiation by x-ray is helpful in controlling nonresectable disease; and 3) The inguinal lymph nodes should be the object of careful clinical evaluation in every patient and probably should be biopsied in all cases. The presence of positive nodes is an indication ...

[Human recombinant erythropoietin-alpha increases the efficacy of irradiation in preclinical model.]

Magyar Onkologia — Fri, 13 Apr 2007 10:25:03 +0100

Flavonoids inhibit breast cancer resistance protein-mediated drug resistance: transporter specificity and structure–activity relationship

Cancer Chemotherapy and Pharmacology — Thu, 08 Mar 2007 08:00:44 +0100

Conclusion??Our present results thus indicate that many flavonoids selectively inhibit BCRP only. Moreover, we examined the structure?BCRP inhibitory activity relationship from our current study. Content TypeJournal Article JournalCancer Chemotherapy and PharmacologyOnline ISSN 1432-0843Print ISSN 0344-5704 (Source: Cancer Chemotherapy and Pharmacology)

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Annexin 1: Differential expression in tumor and mast cells in human larynx cancer

International Journal of Cancer — Mon, 05 Mar 2007 05:00:00 +0100

Annexin 1 protein (ANXA1) expression was evaluated in tumor and mast cells in human larynx cancer and control epithelium. The effect of the exogenous ANXA1 (peptide Ac 2-26) was also examined during the cellular growth of the Hep-2 human larynx epidermoid carcinoma cell line. This peptide inhibited the proliferation of the Hep-2 cells within 144 hr. In surgical tissue specimens from 20 patients with larynx cancer, ultrastructural immunocytochemistry analysis showed in vivo down-regulation of ANXA1 expression in the tumor and increased in mast cells and Hep-2 cells treated with peptide Ac2-26. Combined in vivo and in vitro analysis demonstrated that ANXA1 plays a regulatory role in laryngeal cancer cell growth. We believe that a better understanding of the regulatory mechanisms of ANXA1 in ...

Lung cancer in patients with tuberculosis

BioMed Central — Mon, 19 Feb 2007 07:00:00 +0100

Conclusions: Coexistence of tuberculosis and lung cancer in thoracic surgery is fairly rare. This combination was diagnosed only in 46 cases (2.1%) out of 2218 operated lung cancer patients. Epidermoid carcinoma and stage IIIA disease was diagnosed in 50% of patients. Postoperative surgical complications occurred in 9 patients (19.5%) with lung cancer and tuberculosis. Six patients (13%) died in postoperative period. Surgery is the method of choice in treatment of combination of tuberculosis and lung cancer. Median survival of these patients was 28+/-2 months. (Source: BioMed Central)

Grape seed proanthocyanidins promote apoptosis in human epidermoid carcinoma A431 cells through alterations in Cdki-Cdk-cyclin cascade, and caspase-3 activation via loss of mitochondrial membrane potential

Experimental Dermatology — Sat, 17 Feb 2007 19:06:24 +0100

Experimental Dermatology Volume 0, Issue 0, Page ???-???. (Source: Experimental Dermatology)

Oridonin inhibited the tyrosine kinase activity and induced apoptosis in human epidermoid carcinoma a431 cells.

Biological and Pharmaceutical Bulletin — Mon, 05 Feb 2007 12:15:03 +0100

In this study, we first investigated the mechanism involved in oridonin-induced cell death in human epidermoid carcinoma A431 cells, which overexpress epidermal growth factor receptor (EGFR). After treatment with various doses of oridonin for 24 h, the majority of A431 cells underwent apoptosis in a time- and dose-dependent manner as measured by an LDH activity-based assay. Treatment with oridonin at various concentrations for 24 h caused significant inhibition on the total tyrosine kinase activities and downregulation of EGFR expression or EGFR phosphorylation. Oridonin significantly affected the localization of EGFR and phosphorylated EGFR on the cell membrane. However, genistein (a well-known tyrosine kinase inhibitor) did not induce apoptotic A431 cell death. Importantly, oridonin exhi...

Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice.

Analytical Biochemistry — Mon, 22 Jan 2007 16:23:02 +0100

In this study, our goal was to identify a reliable assay for targeting agent integrity in vitro that correlated with signal strength in vivo. Binding of IRDye 800CW EGF to intact A431 human epidermoid carcinoma cells was quantified in a microplate assay. Specificity was confirmed by competition with unlabeled EGF or monoclonal antibody blocking. Biological activity of intact and damaged targeting agents relative to unlabeled EGF was determined by binding and stimulation of extracellular signal-regulated kinase (ERK) phosphorylation. Both assays indicated a reduction of up to 60% of the fluorescence intensity with damaged agents. Using a research prototype imaging system optimized for IRDye 800CW detection, we compared the efficacy of intact and damaged targeting agents for imaging subcutan...

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Isolation of Gelsedine-Type Indole Alkaloids from Gelsemium elegans and Evaluation of the Cytotoxic Activity of Gelsemium Alkaloids for A431 Epidermoid Carcinoma Cells.

Journal of Natural Products — Sat, 16 Dec 2006 05:00:00 +0100

M. Kitajima, T. Nakamura, N. Kogure, M. Ogawa, Y. Mitsuno, K. Ono, S. Yano, N. Aimi, and H. TakayamaWeb Release Date: Sat, 16 Dec 2006 00:00:00 EST (Erratum) DOI: 10.1021/np068071k (Source: Journal of Natural Products)

[Epidermoid carcinomas of anal canal treated with radiation therapy and concomitant chemotherapy (5-fluorouracil and cisplatin)]

Cancer radiotherapie — Fri, 01 Dec 2006 05:00:00 +0100

CONCLUSION: We confirm the good results with RT and concomitant chemotherapy. The clinical tumor response after the first course of RT and concomitant chemotherapy is probably the most important predictive factor on the disease-free survival. For patients with T3 or T4 lesion and tumor regression <or=50% after the first course of radiation therapy, surgical non conservative treatment should be discussed. PMID: 17110148 [PubMed - indexed for MEDLINE] (Source: Cancer radiotherapie)

Results of Surgical Salvage after Failed Chemoradiation Therapy for Epidermoid Carcinoma of the Anal Canal

Annals of Surgical Oncology — Thu, 16 Nov 2006 01:31:57 +0100

Conclusions Long-term survival following salvage surgery for persistent or locally recurrent epidermoid carcinoma of the anal canal can be achieved in the majority of patients. However, patients who initially present with node-positive disease and patients who receive a radiation dose of less than 55 Gy as part of their initial chemoradiation therapy regimen have a worse prognosis after radical salvage surgery. Content TypeJournal Article JournalAnnals of Surgical OncologyOnline ISSN 1534-4681Print ISSN 1068-9265 (Source: Annals of Surgical Oncology)

Chemical studies on monoterpenoid indole alkaloids from medicinal plant resources Gelsemium and Ophiorrhiza

Journal of Natural Medicines — Sat, 28 Oct 2006 17:02:48 +0100

Abstract We have proved that the plant of origin of “Yakatsu”, one of the ancient medicines stored in Shosoin Repository, is Gelsemium elegans (Loganiaceae). Exhaustive investigation of the alkaloids in this plant as well as its closely related plant, G. sempervirens, resulted in the isolation of more than 50 compounds, including new gelsedine-, sarpagine-, and yohimbane-type alkaloids. Pharmacological study of representative and new Gelsemium alkaloids revealed that the gelsedine-type alkaloids exhibit potent cytotoxic activity against the A431 human epidermoid carcinoma cell line. It was found that Ophiorrhiza pumila (Rubiaceae) produces a remarkable anti-tumor alkaloid, camptothecin, and its related alkaloids, including new compounds that might be the biogenetic precursors o...

Phosphoinositide 3-Kinase C2beta regulates cytoskeletal organization and cell migration via Rac-dependent mechanisms.

Mol Biol Cell — Thu, 19 Oct 2006 03:10:03 +0100

In this report, we demonstrate that class II phosphoinositide 3-kinase C2beta (PI3KC2beta) associates with the Eps8/Abi1/Sos1 complex and is recruited to the EGF receptor as part of a multiprotein signaling complex also involving Shc and Grb2. Increased expression of PI3KC2beta stimulated Rac activity in A-431 epidermoid carcinoma cells, resulting in enhanced membrane ruffling and migration speed of the cells. Conversely, expression of dominant negative PI3KC2beta reduced Rac activity, membrane ruffling, and cell migration. Moreover, PI3KC2beta-overexpressing cells were protected from anoikis and displayed enhanced proliferation, independently of Rac function. Taken together, these findings suggest that PI3KC2beta regulates the migration and survival of human tumor cells by distinct molecu...

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Unsuspected FDG–PET findings in the follow-up of patients with lymphoma

Annals of Hematology — Thu, 05 Oct 2006 07:27:37 +0100

We report seven patients with a history of lymphoma who presented a positive FDG–PET suggestive of lymphoma relapse and for which FDG–PET oriented biopsies revealed alternative diagnoses. Early in lymphoma follow-up, persistence of focal increased FDG activity corresponded to inflammatory or infectious lesions in two patients: one aspergillosis and one sarcoidosis. Later in the follow-up, five cases of secondary malignancies were identified (three lung cancers, one epidermoid carcinoma, and one villous tumor) in this particularly exposed population. The routine use of FDG PET to evaluate lymphoma significantly increases the probability of detecting unexpected diseases. These cases illustrate the potential pitfalls in PET follow-up. Because FDG is not lymphoma-specific, a relapse suspec...

Berberine inhibits growth, induces G1 arrest and apoptosis in human epidermoid carcinoma A431 cells by regulating Cdki-Cdk-cyclin cascade, disruption of mitochondrial membrane potential and cleavage of caspase 3 and PARP

Carcinogenesis — Mon, 25 Sep 2006 06:00:00 +0100

Chemotherapeutic approach using non-toxic botanicals may be one of the strategies for the management of the skin cancers. Here we report that in vitro treatment of human epidermoid carcinoma A431 cells with berberine, a naturally occurring isoquinoline alkaloid, decreased cell viability (3–77%, P < 0.05–0.001) and induced cell death (3–51%, P < 0.01–0.001) in a dose (5–75 µM)- and time (12–72 h)-dependent manner, which was associated with an increase in G1 arrest. G0/G1 phase of the cell cycle is known to be controlled by cyclin dependent kinases (Cdk), cyclin kinase inhibitors (Cdki) and cyclins. Our western blot analysis showed that berberine-induced G1 cell cycle arrest was mediated through the increased expression of Cdki proteins (Cip1/p...

Regulated Shedding of Syndecan Ectodomains by Chemokines

TheScientificWorldJOURNAL: Newly published articles. — Mon, 11 Sep 2006 14:07:02 +0100

Chemokines are involved in host defense but also in cancer cell proliferation and migration. We characterized a cell proteoglycan, syndecan-4, which binds to the chemokine, SDF-1/CXCL12 (Stromal Cell-derived Factor-1) and demonstrated that SDF-1/CXCL12 accelerates the shedding of the ectodomains of syndecan-4 and to a lesser extent that of another proteoglycan, syndecan-1, from human epidermoid carcinoma cells and from human primary macrophages. This may represent fine regulatory mechanisms in physiology, and diseases: inflammation and cancer. (Source: TheScientificWorldJOURNAL: Newly published articles.)

Muco-epidermoid carcinoma of the breast

Pathology International — Thu, 24 Aug 2006 09:16:05 +0100

Pathology International Volume 56, Issue 9, Page 549-553, Sep 2006. (Source: Pathology International)

Molecular Imaging of EGFR Kinase Activity in Tumors with 124I-Labeled Small Molecular Tracer and Positron Emission Tomography

Molecular Imaging and Biology — Wed, 16 Aug 2006 07:12:52 +0100

Abstract Positron emission tomography (PET) with epidermal growth factor receptor (EGFR) kinase-specific radiolabeled tracers could provide the means for noninvasive and repetitive imaging of heterogeneity of EGFR expression and signaling activity in tumors in individual patients before and during therapy with EGFR signaling inhibitors. We developed the synthesis and 124I-radiolabeling of the (E)-But-2-enedioic acid [4-(3-[124I]iodoanilino)-quinazolin-6-yl]-amide-(3-morpholin-4-yl-propyl)-amide (morpholino-[124I]-IPQA), which selectively, irreversibly, and covalently binds the adenosine-triphosphate-binding site to the activated (phosphorylated) EGFR kinase, but not to the inactive EGFR kinase. The latter was demonstrated using in silico modeling with crystal structures of the wild type...

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Resveratrol inhibits proliferation of human epidermoid carcinoma A431 cells by modulating MEK1 and AP-1 signalling pathways

Experimental Dermatology — Fri, 02 Jun 2006 08:27:53 +0100

Experimental Dermatology Volume 15, Issue 7, Page 538-546, Jul 2006 (Source: Experimental Dermatology)

Correlation between TNM classification and malignancy histological feature of oral squamous cell carcinoma.

Revista Brasileira de Otorrinolaringologia — Mon, 14 Mar 2005 07:00:00 +0100

CONCLUSION: TNM classification, as well as malignancy mean score, had statistically significant correlation with degree of keratinization, nuclear pleomorphism and lymphocytic infiltration. These highly significant results indicated that histologically invasive areas may be primarily responsible for the clinical behavior of the tumor, and this may be important for the therapy of choice for oral squamous cell carcinoma. PMID: 16446915 [PubMed - indexed for MEDLINE] (Source: Revista Brasileira de Otorrinolaringologia)