MedWorm: Epidermoid Carcinoma

Surgical Therapy for Epidermoid Carcinoma of the Anal Canal: A NSQIP Assessment of Short-term Outcomes

Journal of Surgical Research — Sat, 14 Jan 2012 22:28:54 +0100

(Source: Journal of Surgical Research)

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Anogenital pseudotumoral herpes and HIV infection: a new challenge for diagnosis and treatment.

Herpes — Wed, 04 Jan 2012 05:00:00 +0100

Anogenital pseudotumoral herpes and HIV infection: a new challenge for diagnosis and treatment. AIDS. 2012 Jan 4; Authors: Lucca-Chrisment Julie DI, Simon J, Ludivine G, Françoise P, Hélène L, Flore R, Jean-Pierre M, Alexiane D, Marie-Françoise A, Nicolas D Abstract HIV-infected patients may develop rare anogenital pseudotumoral herpes potentially mimicking epidermoid carcinoma. We assessed treatment in five new cases with a median follow-up of 3.3 years. Recurrence and clinical nucleoside analog resistance were observed in all patients. All drug treatments were only temporarily curative and clinical responses varied between patients and recurrences. Foscavir seemed to be the most appropriate second-line treatment and cidofovir or thalidomide should be considered as alt...

2‐Amino‐3‐cyano‐4‐(5‐arylisoxazol‐3‐yl)‐4H‐chromenes: Synthesis and In Vitro Cytotoxic Activity

Archiv der Pharmazie — Sun, 01 Jan 2012 05:00:00 +0100

AbstractA new series of 4‐aryl‐4H‐chromenes bearing a 5‐arylisoxazol‐3‐yl moiety at the C‐4 position were prepared as potential anticancer agents. The in vitro cytotoxic activity of the synthesized compounds was investigated against a panel of tumor cell lines including MCF‐7 (breast cancer), KB (nasopharyngeal epidermoid carcinoma), Hep‐G2 (liver carcinoma), MDA‐MB‐231 (breast cancer), and SKNMC (human neuroblastoma) using the MTT colorimetric assay. Doxorubicin, a well‐known anticancer drug, was used as positive standard drug. Among the synthesized compounds, the 5‐(3‐methylphenyl)isoxazol‐3‐yl analog (7j) showed the most potent cytotoxic activity against all five human tumor cell lines.A series of 2‐amino‐3‐cyano‐4‐(5‐arylisoxazol‐3‐yl)‐...

SNX3-dependent regulation of epidermal growth factor receptor (EGFR) trafficking and degradation by aspirin in epidermoid carcinoma (A-431) cells.

Cellular and Molecular Life Sciences : CMLS — Sun, 11 Dec 2011 05:00:00 +0100

This study sheds light on how aspirin may down-regulate surface expression of EGFR by inhibiting/delaying the exit of endocytosed-EGFR from the ESE and recycling of endocytosed-EGFR back to the cell surface. PMID: 22159558 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Life Sciences : CMLS)

Primary epidermoid carcinoma of the breast presenting as a breast abscess and sepsis.

Sao Paulo Medical Journal — Thu, 01 Dec 2011 05:00:00 +0100

CONCLUSIONS: This unusual case illustrates how an apparently benign disorder such as a breast abscess might be related to a clinically occult malignancy. A review of the literature on SCC of the breast is presented. PMID: 22249799 [PubMed - in process] (Source: Sao Paulo Medical Journal)

Radiotherapy‐induced pemphigus vulgaris with autoantibodies targeting a 110 kDa epidermal antigen

International Journal of Dermatology — Tue, 22 Nov 2011 05:52:39 +0100

Conclusion Our patient showed some distinctive findings never reported previously in RIP: a histological focal keratinocyte necrosis, and the presence of autoantibodies reacting with a 110 kDa keratinocytic protein in immunoblot analysis. Because of a different prognosis, it is important to differentiate RIP and paraneoplastic pemphigus (PNP), although cases of ionizing radiation‐induced PNP had also been described. As in our patient, RIP seems to respond well to systemic corticosteroids and immunosuppressive therapy, which induce remission within a few months. (Source: International Journal of Dermatology)

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Isolation of Coxsackievirus A24 variant from patients with hemorrhagic conjunctivitis in Cuba, 2008–2009

Journal of Clinical Virology — Thu, 10 Nov 2011 05:00:00 +0100

Conclusions: Outbreaks of acute hemorrhagic conjunctivitis occurred in Cuba in 2008 and 2009 were caused by Coxsackievirus A24 variant. The faecal-oral route is another mode of transmission of CA24v in the acute hemorrhagic conjunctivitis outbreaks. Phylogenetic analysis of Cuban CA24v strains involved in an acute hemorrhagic conjunctivitis outbreak in 2008 and 2009 confirms a new introduction of the CA24 variant into the Americas from South-east Asia. (Source: Journal of Clinical Virology)

Evaluation of antioxidative, protective effect against H(2)O(2) induced cytotoxicity, and cytotoxic activities of three different Quercus species.

Food and Chemical Toxicology — Mon, 31 Oct 2011 04:00:00 +0100

Authors: Söhretoğlu D, Sabuncuoğlu S, Harput US Abstract Quercus species are used as antidiarrheic, for the treatment of hemorrhoid, oral and anal mucosa inflammation. These tree species have been of interest to researchers because of their usage in folk medicine, consumption as food, beverage and especially usage of oak woods for construction in wine barrels. The DPPH, SO and NO radical scavenging activities, protective effect against H(2)O(2) induced cytotoxicity as well as their cytotoxic activity against Hep-2 human larynx epidermoid carcinoma cell line of the MeOH and water extracts of the barks of Quercus cerris var. cerris, Quercusmacranthera subsp. syspirensis and Quercus aucheri were investigated for the first time. Total phenolic content of the extracts was also evalua...

Novel cytotoxic chalcones from Litsea rubescens and Litsea pedunculata.

Bioorganic and Medicinal Chemistry Letters — Sat, 08 Oct 2011 04:00:00 +0100

Authors: Li L, Zhao XT, Luo YP, Zhao JF, Yang XD, Zhang HB Abstract Two novel flavonoids with chalcone skeleton, together with seven known flavonoids, were isolated from the stem barks of Litsea rubescens and Litsea pedunculata. The structures of the new compounds were elucidated on the basis of spectral methods including IR, UV, 1D and 2D NMR. The new chalcones were found to contain the rare epoxy or ethylidenedioxy group. This is the first report on the presence of chalcone in the plant genus Litsea. The cytotoxic potential of two new chalcones was evaluated in vitro against three human tumor cell lines. Both new chalcones displayed potent cytotoxic activities against myeloid leukaemia (HL-60) and epidermoid carcinoma (A431) cell lines and more active than cisplatin (DDP). Intere...

Reliability of pet-scan for presurgical staging in oral cancer: a prospective study

International Journal of Oral and Maxillofacial Surgery — Sat, 01 Oct 2011 04:00:00 +0100

Introduction: The Oral Cavity Epidermoid Carcinoma (OCEC) represents 4–6% of human malignant tumours. Although CT and MRI still play a major role in diagnosing such metastatic nodes presurgically, the Positrons Emission Tomography (PET), have been introduced in our practice and seems to improve its validity. (Source: International Journal of Oral and Maxillofacial Surgery)

Novel Terpenoids from Calocedrus macrolepis var. formosana.

Chemistry and Biodiversity — Sat, 01 Oct 2011 04:00:00 +0100

Authors: Hsieh CL, Tseng MH, Pan RN, Chang JY, Kuo CC, Lee TH, Kuo YH Abstract Calocetriol (1), diacetylcalocediol (2), and ferrugimenthenol (3) were isolated from the bark of Calocedrus macrolepis var. formosana. Among them, 1 and 2 are secoabietane-type diterpenoids, and 3, with a novel C(20) C(10) skeleton, is classified as a meroterpenoid. The structures of 1-3 were elucidated by spectroscopic analyses, and their biological activities were also evaluated. Compound 3 exhibited significant cytotoxic activity against human oral epidermoid carcinoma KB cells with an IC(50) value of 9.0±0.1 μM. PMID: 22006718 [PubMed - in process] (Source: Chemistry and Biodiversity)

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Maspin and MCM2 immunoprofiling in Salivary Gland Carcinomas

Diagnostic Pathology — Mon, 26 Sep 2011 04:00:00 +0100

Conclusions: Salivary gland carcinomas express maspin and MCM2 with variable levels and cellular localization, consisting important markers of biological behavior in these tumors. The level of MCM2 expression can be used in the differential diagnosis of adenoid cystic carcinoma and polymorphous low grade adenocarcinoma. Further study with large sample size is recommended to assess their value in prediction of lymph node metastasis. (Source: Diagnostic Pathology)

Novel glitazones: glucose uptake and cytotoxic activities, and structure–activity relationships

Medicinal Chemistry Research — Wed, 21 Sep 2011 05:50:05 +0100

Abstract In this article, two series of novel glitazones are reported for their synthesis, in vitro glucose uptake activity by the rat hemi-diaphragm, and in vitro cytotoxic activity against human carcinoma HEp-2 (epidermoid carcinoma of the larynx) and A549 (human lung cancer cell line) cells. Some of the compounds exhibit good glucose uptake and cytotoxic activities. Compound 5 with 2,4-dichlorophenol moiety connected to TZD via the two carbon linker has exhibited good glucose uptake activity. One of the compounds exhibits both cytotoxic and glucose uptake activities (compound 4). Illustrations about their synthesis, and in vitro glucose uptake and cytotoxic activities are described along with their structure–activity relationships. Graphical abstract &nbs...

[Tongue reconstruction with a bilateral infrahyoid flap innervated by Ansa Cervicalis after total glossectomy.]

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Wed, 21 Sep 2011 04:00:00 +0100

We present the larynx sparing bilateral infrahyoid flap reconstruction procedure after total glossectomy. PATIENTS AND METHOD: Three patients managed for an epidermoid carcinoma of the tongue, classified T4N0, underwent total glossectomy. The tongue was reconstructed with a bilateral infrahyoid flap pedicled on two superior thyroid arteries and innervated by Ansa Cervicalis. RESULTS: Oral food intake was resumed after 8 to 20 days. No false route was observed. The muscular flap mobility was clinically satisfactory. It was assessed by EMG in one case. Esophageal transit confirmed the absence of stasis and false route for one patient. DISCUSSION: This short series proves the feasibility of bilateral innervated and pedicled infrahyoid flap procedure. It is an alternative to volumetric and fu...

[Induction chemotherapy for head and neck epidermoid carcinomas.]

Cancer Radiotherapie — Mon, 29 Aug 2011 23:00:00 +0100

Authors: Peyrade F, Saâda E, Benezery K, Hebert C, Dassonville O Abstract The standard treatment for head and neck inoperable squamous cell carcinoma is an association of radiotherapy and platinum. However, only one patient out of three remains alive five years after diagnosis. The interest in induction chemotherapy was renewed by the introduction of taxanes combined with cisplatinum and 5-fluoro-uracile. The triple association taxane-cisplatinum-5-fluoro-uracile yielded improved survival when compared to cisplatinum-5-fluoro-uracile. Wider use of taxane-cisplatinum-5-fluoro-uracile is limited by its toxicity and the lack of randomized comparison with a concomitant chemoradiotherapy scheme including optimal doses of platinum. Until the results of new phase III trials are published...

Nuclear translocation of Sgt1 depends on its phosphorylation state.

The International Journal of Biochemistry and Cell Biology — Mon, 15 Aug 2011 23:00:00 +0100

Authors: Prus W, Zabka M, Bieganowski P, Filipek A Abstract Recently we have shown that the Sgt1 (suppressor of G2 allele of Skp1) protein translocates to the nucleus due to heat shock and that the Ca(2+)-bound form of S100A6 is required for Sgt1 translocation (Prus and Filipek, 2010). In this work we studied the influence of Sgt1 phosphorylation on nuclear translocation. By means of two-dimensional (2D) electrophoresis we showed that in the protein extract of heat-shocked human epidermoid carcinoma (HEp-2) cells a higher level of a basic, most probably non-phosphorylated, form of Sgt1 can be detected. Also, we found a more efficient translocation of Sgt1 induced by heat shock when casein kinase II inhibitor was added to the cells. To confirm the role of Sgt1 phosphorylation/dephos...

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[Proliferating trichilemmal tumor of the ischiorectal fossa].

Annales de Pathologie — Sun, 31 Jul 2011 23:00:00 +0100

We report a case of PTT localized in the ischiorectal fossa, which might have been diagnosed as an epidermoid carcinoma. PMID: 21839360 [PubMed - in process] (Source: Annales de Pathologie)

Endoscopic endonasal transpterygoid nasopharyngectomy

The Laryngoscope — Tue, 19 Jul 2011 23:00:00 +0100

Conclusion:Endoscopic transpterygoid nasopharyngectomy for primary and recurrent nasopharyngeal malignancies is feasible and safe in properly selected patients. Preliminary outcomes compare to that of conventional techniques. Endoscopic resections, however, are demanding; they require specialized equipment and a team versed in endoscopic oncologic surgery. Long‐term follow‐up and reproducibility remain undefined. Laryngoscope, 2011. (Source: The Laryngoscope)

Histology of submandibular gland tumours, 10 years' experience.

Acta Otorrinolaringologica Espanola — Mon, 11 Jul 2011 23:00:00 +0100

CONCLUSIONS: A greater proportion of benign neoplasm was found in submandibular tumours. The most frequent benign tumour is the pleomorphic adenoma. Women are more commonly affected (76%). Benign tumours appear in younger patients than do malignant ones. PMID: 21757179 [PubMed - as supplied by publisher] (Source: Acta Otorrinolaringologica Espanola)

Eudistomidins H-K, new β-carboline alkaloids from the Okinawan marine tunicate Eudistoma glaucus.

Bioorganic and Medicinal Chemistry Letters — Tue, 05 Jul 2011 01:17:02 +0100

Authors: Suzuki T, Kubota T, Kobayashi J Four new β-carboline alkaloids, eudistomidins H-K (1-4), were isolated from an Okinawan marine tunicate Eudistoma glaucus and the structures of 1-4 were elucidated on the basis of spectroscopic data. Eudistomidins H (1) and I (2) were new β-carboline alkaloids possessing a unique fused-tetracyclic ring system consisting of a tetrahydro β-carboline ring and a hexahydropyrimidine ring. Eudistomidin J (3) showed relatively potent cytotoxicity against murine leukemia cells P388 and L1210, and human epidermoid carcinoma cells KB in vitro. PMID: 21684745 [PubMed - in process] (Source: Bioorganic and Medicinal Chemistry Letters)

Antitumor activity of HM781‐36B, a highly effective pan‐HER inhibitor in erlotinib‐resistant NSCLC and other EGFR‐dependent cancer models

International Journal of Cancer — Mon, 04 Jul 2011 23:00:00 +0100

In this study, we evaluated the therapeutic potential of a novel, irreversible pan‐HER inhibitor, HM781‐36B. The results from this study show that HM781‐36B is a potent inhibitor of EGFR in vitro, including the EGFR‐acquired resistance mutation (T790M), as well as HER‐2 and HER‐4, compared with other EGFR tyrosine kinases inhibitors (erlotinib, lapatinib and BIBW2992). HM781‐36B treatment of EGFR DelE746_A750‐harboring erlotinib‐sensitive HCC827 and EGFR L858R/T790M‐harboring erlotinib‐resistant NCI‐H1975 NSCLC cells results in the inhibition of EGFR phosphorylation and the subsequent deactivation of downstream signaling proteins. Additionally, HM781‐36B shows an excellent efficacy in a variety of EGFR‐ and HER‐2‐dependent tumor xenograft models, including e...

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In Vitro Cytotoxic Activity and Structure Activity Relationships of Iridoid Glucosides Derived from Veronica species

Phytotherapy Research — Wed, 15 Jun 2011 23:00:00 +0100

In conclusion, iridoid glucosides are considered to show a biphasic effect on cancer cells that is both cytostatic and cytotoxic, depending on the chemical structure and the type of cancer cell. Copyright © 2011 John Wiley & Sons, Ltd. (Source: Phytotherapy Research)

Inhibition of the EGF receptor and ERK1/2 signaling pathways rescues the human epidermoid carcinoma A431 cells from IFNγ-induced apoptosis.

Cell Cycle — Fri, 27 May 2011 01:46:35 +0100

In this study, we showed that IFNγ promoted progressive death in A431 cells, overexpressing EGF receptor (EGFR). Based on the data provided by evaluating cell morphology, MTT assay, FACS analysis, and cleaved caspase-3 staining we concluded that the major cause of IFNγ-induced A431 cell growth inhibition was not cell cycle arrest, but apoptosis. We investigated a role for the EGFR and ERK1/2 MAPK signaling pathways in IFNγ-induced apoptosis of A431 cells. IFNγ-induced cell death was accompanied by both an increase of the ERK1/2 MAPK activation and a simultaneous reduction of the EGFR activation. Activation of ERK1/2 was crucial for IFNγ-induced cell death because MEK1/2 inhibitors, PD0325901 and U0126 efficiently protected cells from apoptosis by suppressing caspase-3 activation. Even...

Outcomes of salvage surgery for epidermoid carcinoma of the anus following failed combined modality treatment

American Journal of Surgery — Sat, 30 Apr 2011 23:00:00 +0100

Conclusions: Recurrent anal carcinoma after primary chemoradiotherapy carries a poor prognosis. Salvage abdominoperineal resection offers a potential for long-term survival. (Source: American Journal of Surgery)

Fischerisin A and B, Cytotoxic Sesquiterpenoid-Geranylhydroquinones from Ligularia fischeri.

Chemical and Pharmaceutical Bulletin — Sat, 16 Apr 2011 19:15:31 +0100

Authors: Xie WD, Li X, Weng CW, Liu SS, Row KH During the course of screening natural sesquiterpenoids for new antitumor agents, two novel compounds, fischerisin A (1) and fischerisin B (2), were isolated from the roots of Ligularia fischeri. Their structures were elucidated by interpretation of their IR, high resolution-mass spectrometry (HR-MS), 1D- and 2D-NMR data. Fischerisin A and B are the first representatives of a novel sesquiterpenoid-geranylhydroquinone hybrid, and both compounds exhibited in vitro cytotoxicity against cultured human oral epidermoid carcinoma (KB) and human breast cancer (MCF-7) cell lines with IC(50) values of 9.7 and 10.2 µM, and 9.8 and 17.8 µM, respectively. PMID: 21467686 [PubMed - in process] (Source: Chemical and Pharmaceutical Bulletin)

Pregnane steroidal glycosides and their cytostatic activities

Glycobiology — Tue, 05 Apr 2011 23:00:00 +0100

Four new steroidal glycosides such as 3-O-6-deoxy-3-O-methyl-β-d-allopyranosyl-(1 -> 4)-β-d-oleandropyranosyl-(1 -> 4)-β-d-cymaropyranosyl-(1 -> 4)-β-d-cymaropyranoside-12-β-tigloyl-14-β-hydroxy-17-β-pregnane (1), 3-O-6-deoxy-3-O-methyl-β-d-allopyranosyl-(1 -> 4)-β-d-oleandropyranosyl-(1 -> 4)-β-d-cymaropyranosyl-(1 -> 4)-β-d-cymaropyranoside-12-β-(2'-amino)-benzoyl-14-β-hydroxy-17-β-pregnane (2), 3-O-6-deoxy-3-O-methyl-β-d-allopyranosyl-(1 -> 4)-β-d-oleandropyranosyl-(1 -> 4)-β-d-cymaropyranosyl-(1 -> 4)-β-d-cymaropyranoside-12-β-14-β-dihydroxy-17-α-pregnane (3) and 3-O...

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(-)-Epigallocatechin-3-gallate reactivates silenced tumor suppressor genes, Cip1/p21 and p16INK4a, by reducing DNA methylation and increasing histones acetylation in human skin cancer cells

Carcinogenesis — Mon, 28 Mar 2011 23:00:00 +0100

The anti-skin carcinogenic effects of green tea catechins have been studied extensively in vitro and in vivo models but the precise epigenetic molecular mechanisms are still unclear. Accumulating data suggest that dietary phytochemicals may alter cancer risk by modifications of epigenetic processes in the cells. The present study was designed to investigate whether tea catechins, particularly (–)-epigallocatechin-3-gallate (EGCG), would modify epigenetic events to regulate DNA methylation-silenced tumor suppressor genes in skin cancer cells. DNA methylation, histone modifications and tumor suppressor gene expressions were studied in detail using human epidermoid carcinoma A431 cells as an in vitro model after EGCG treatment using cytostaining, western blotting, dot blot analysis, rea...

Bromelain inhibits nuclear factor kappa‐B translocation, driving human epidermoid carcinoma A431 and melanoma A375 cells through G2/M arrest to apoptosis

Molecular Carcinogenesis — Tue, 22 Mar 2011 00:00:00 +0100

AbstractBromelain, obtained from pineapple, is already in use clinically as adjunct in chemotherapy. Our objective was to test its ability to act as a sole anti‐cancer agent. Therefore, we describe its anti‐proliferative, anti‐inflammatory and subsequent anti‐cancer effects in vitro, against human epidermoid carcinoma‐A431 and melanoma‐A375 cells. Bromelain exhibited reduction in proliferation of both these cell‐lines and suppressed their potential for anchorage‐independent growth. Further, suppression of inflammatory signaling by bromelain was evident by inhibition of Akt regulated‐nuclear factor‐kappaB activation via suppression of inhibitory‐kappaBα phosphorylation and concomitant reduction in cyclooxygenase‐2. Since, the inflammatory cascade is well‐known to ...

Divalent lead cations induce cyclooxygenase-2 gene expression by epidermal growth factor receptor/nuclear factor-kappa B signaling in A431carcinoma cells.

Toxicology Letters — Tue, 22 Mar 2011 00:00:00 +0100

Authors: Chou YH, Woon PY, Huang WC, Shiurba R, Tsai YT, Wang YS, Hsieh TJ, Chang WC, Chuang HY, Chang WC Divalent lead cations (Pb(2+)) are toxic metal pollutants that may contribute to inflammatory diseases in people and animals. Human vascular smooth muscle cells in culture respond to low concentrations of Pb(2+) ions by activating mediators of inflammation via the plasma membrane epidermal growth factor receptor (EGFR). These include cyclooxygenase-2 (COX-2) and cytosolic phospholipase A(2) as well as the hormone-like lipid compound prostaglandin E(2). To further clarify the mechanism by which Pb(2+) induces such mediators of inflammation, we tested human epidermoid carcinoma cell line A431 that expresses high levels of EGFR. Reverse transcription PCR and western blots confirmed A4...

Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells

Journal of Oncology — Tue, 01 Mar 2011 17:29:16 +0100

In conclusion, these results suggested that LIEAF inhibited cervical cancer cells growth by inducing apoptosis and could be developed as potential anticancer drugs. (Source: Journal of Oncology)

Structure-Function Analysis of Tetraspanin CD151 [Cell Biology]

Journal of Biological Chemistry — Fri, 25 Feb 2011 00:00:00 +0100

The basement membrane protein laminin-332 (laminin-5) mediates both stable cell adhesion and rapid cell migration and thus has the potential to either restrain or promote tumor cell metastasis. The major cellular receptors for laminin-332 are integrin α3β1, which mediates rapid tumor cell migration, and integrin α6β4, which often mediates stable cell attachment. Tetraspanin protein CD151 interacts directly with both α3β1 and α6β4 integrins and with other tetraspanins, thereby promoting α3β1 and α6β4 association with tetraspanin-enriched microdomains on the cell surface. To explore the possibility of selectively modulating tumor cell responses to laminin-332, we re-expressed a series of CD151 mutants in epidermoid carcinoma cells with near total, RNAi-mediated silencing of endog...

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Critical role of Wnt5a–Ror2 signaling in motility and invasiveness of carcinoma cells following Snail‐mediated epithelial–mesenchymal transition

Genes to Cells — Wed, 23 Feb 2011 22:51:34 +0100

Expression of Snail has been shown to mediate epithelial–mesenchymal transition (EMT) of epithelial cells and carcinomas, characterized by morphological alterations with disappearance and appearance of E‐cadherin and vimentin, respectively. Here, we show that ectopic expression of Snail in human epidermoid carcinoma A431 cells (Snail/A431) induces the representative EMT, resulting in remarkable motile and invasive properties of the cells. Expression of Wnt5a, its receptor Ror2 and matrix metalloproteinase (MMP)‐2 is induced in Snail/A431, but not in control A431 cells. Interestingly, suppressed expression of either Wnt5a or Ror2 in Snail/A431 cells results in the inhibition of in vitro cell motility and invasiveness, at least partly mediated by MMP‐2, without affecting characterist...

New norditerpenoid alkaloids from Scutellaria barbata with cytotoxic activities.

Natural Product Research — Tue, 15 Feb 2011 00:00:00 +0100

Authors: Dai SJ, Peng WB, Shen L, Zhang DW, Ren Y Two new norditerpenoid alkaloids, named scutebarbatines M-N (1 and 2), were isolated from the whole plant of Scutellaria barbata D. Don. Their structures were established on the basis of detailed spectral analyses. In vitro, two new compounds showed significant cytotoxic activities against three human cancer lines (HONE-1 nasopharyngeal, KB oral epidermoid carcinoma and HT29 colorectal carcinoma cells), and gave IC(50) values in the range of 3.5-6.3 µM. PMID: 21337255 [PubMed - as supplied by publisher] (Source: Natural Product Research)

Epidermoid carcinoma complicating esophageal lichen planus without cutaneous or oral involvement

Gastrointestinal Endoscopy — Fri, 04 Feb 2011 00:00:00 +0100

We report the first observation of a carcinoma that developed on an esophageal lichen planus in a patient without skin or oral lichen. (Source: Gastrointestinal Endoscopy)

Melatonin and cancer: current knowledge and its application to oral cavity tumours

Journal of Oral Pathology and Medicine — Tue, 01 Feb 2011 00:00:00 +0100

Conclusions: MLT contributes to protecting the oral cavity from tissue damage caused by receptor action. Experimental evidence suggests that it may be useful in the treatment and prognosis of tumour processes in the oral cavity. (Source: Journal of Oral Pathology and Medicine)

Synthesis and preliminary evaluation of curcumin analogues as cytotoxic agents.

Bioorganic and Medicinal Chemistry Letters — Tue, 25 Jan 2011 13:03:52 +0100

Authors: Zhang Q, Zhong Y, Yan LN, Sun X, Gong T, Zhang ZR A series of curcumin analogues with different substituents at the 4-position of the phenyl group were synthesized and screened for in vitro cytotoxicity against a panel of human cancer cell lines. Several novel curcumin analogues, especially 32 and 34, exhibited selective and potent cytotoxic activity against human epidermoid carcinoma cell line A-431 and human glioblastoma cell line U-251, implying their specific potential in the chemoprevention and chemotherapy of skin cancer and glioma. The preliminary SAR information extracted from the results suggested that introduction of appropriate substituents to the 4'-positions could be a promising approach for the development of new cytotoxic curcumin analogues with special selectiv...

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Toca-1 Regulates Cell Motility [Molecular Bases of Disease]

Journal of Biological Chemistry — Fri, 14 Jan 2011 00:00:00 +0100

Toca-1 (transducer of Cdc42-dependent actin assembly) interacts with the Cdc42·N-WASP and Abi1·Rac·WAVE F-actin branching pathways that function in lamellipodia formation and cell motility. However, the potential role of Toca-1 in these processes has not been reported. Here, we show that epidermal growth factor (EGF) induces Toca-1 localization to lamellipodia, where it co-localizes with F-actin and Arp2/3 complex in A431 epidermoid carcinoma cells. EGF also induces tyrosine phosphorylation of Toca-1 and interactions with N-WASP and Abi1. Stable knockdown of Toca-1 expression by RNA interference has no effect on cell growth, EGF receptor expression, or internalization. However, Toca-1 knockdown cells display defects in EGF-induced filopodia and lamellipodial protrusions compared with co...

A nanoemulsion formulation of dacarbazine reduces tumor size in a xenograft mouse epidermoid carcinoma model compared to dacarbazine suspension

Nanomedicine : Nanotechnology, Biology, and Medicine — Thu, 06 Jan 2011 00:00:00 +0100

Abstract: Dacarbazine (DAC) is an anticancer drug that has been used to treat various types of cancers. The aim of the current study was to test whether there is an increased efficacy of DAC as a nanoemulsion on reducing tumor size in an epidermoid carcinoma xenograft mouse model. Tumors were induced in 5-week-old nude mice by subcutaneous injection. The mice were untreated or treated with a suspension of DAC (0.1 mg/kg), a nanoemulsion of DAC (0.1 mg/kg), or Nano-Control (same composition as the suspension and nanoemulsion but no DAC), every 2 days by either intramuscular injection (IM) or topical application. After 40 days, the final tumor size of mice receiving the nanoemulsion of DAC IM (0.83 ± 0.55 mm3) was significantly reduced compared to the suspension of DAC IM (4.75 ± 0.49 mm3)...

Aggressive growth and neoplastic potential of dentigerous cysts: With particular reference to central mucoepidermoid carcinoma

The British Journal of Oral and Maxillofacial Surgery — Mon, 03 Jan 2011 00:00:00 +0100

Abstract: Epidermoid carcinoma and mucoepidermoid carcinoma that arise centrally within the jaws are often associated with dental cysts. Central mucoepidermoid carcinoma of the jaws is rare, and usually presents in the fourth to eighth decades; we know of only 7 cases that have been described in children younger than 16. Here we describe a central mucoepidermoid carcinoma in an 8-year-old girl that arose from a dentigerous cyst associated with an unerupted mandibular molar. (Source: The British Journal of Oral and Maxillofacial Surgery)

Cytoplasmic expression of survivin is an independent predictor of poor prognosis in patients with salivary gland cancer

Histopathology — Mon, 01 Nov 2010 00:00:00 +0100

Conclusions: The correlation between cytoplasmic survivin expression and survival in salivary gland malignancies might make this an effective tool in patient follow‐up, prognosis and targeted therapy in future. (Source: Histopathology)

[Partial laryngectomy in supraglottic pharyngeal tumors.]

Cirugia y Cirujanos — Mon, 01 Nov 2010 00:00:00 +0100

Conclusions: We describe two surgical techniques designed to obtain adequate cancer control, preserving the natural functions of swallowing, speech and ventilation in patients with tumors arising in the supraglottic region and the region between the larynx and hypopharynx. PMID: 21214994 [PubMed - as supplied by publisher] (Source: Cirugia y Cirujanos)

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Spanish (Chile)

International Journal of Oral and Maxillofacial Surgery — Sun, 24 Oct 2010 01:46:49 +0100

Partial laryngectomy for early laryngeal cancer: a 25-year experienceC. L Celedón, M. F. Royer, L. B. Ortúzar Rev Otorrinolaringol Cir Cabeza Cuello 2008: 68: 117–123Partial laryngectomy and radiotherapy are good alternatives for early laryngeal cancer treatment, as they are similar in both survival rate and laryngeal preservation. The first author's 25-year experience on this subject was presented in this article. A retrospective review of early laryngeal cancer patients treated between 1980 and 2005 was done. History, type of treatment, tumour histology, complications and survival rates were analysed. 96 patients were recruited with an average age of 58 years. The most frequent histological type was epidermoid carcinoma (92%). 53% of patients had partial laryngectomy, and 47% had ra...

Can nuclear magnetic resonance (NMR) spectroscopy reveal different metabolic signatures for lung tumours?

Virchows Archiv — Wed, 13 Oct 2010 05:50:16 +0100

This study aims to evaluate the potential of 1H NMR spectroscopy, combined with multivariate statistics, for discriminating between tumour and non-involved (control) pulmonary parenchyma and for providing biochemical information on different histological types. Paired tissue samples from 24 primary lung tumours were directly analysed by high-resolution magic angle spinning (HRMAS) 1H NMR spectroscopy (500 MHz), and their spectral profiles subjected to principal component analysis (PCA) and partial least squares regression discriminant analysis (PLS-DA). Tumour and adjacent control parenchyma were clearly discriminated in the PLS-DA model with a high level of sensitivity (95% of tumour samples correctly classified) and 100% specificity (no false positives). The metabolites giving ...

Antitumor Activity of IMC-038525, a Novel Oral Tubulin Polymerization Inhibitor.

Translational Oncology — Sat, 09 Oct 2010 17:05:03 +0100

Authors: Tuma MC, Malikzay A, Ouyang X, Surguladze D, Fleming J, Mitelman S, Camara M, Finnerty B, Doody J, Chekler EL, Kussie P, Tonra JR Microtubules are a well-validated target for anticancer therapy. Molecules that bind tubulin affect dynamic instability of microtubules causing mitotic arrest of proliferating cells, leading to cell death and tumor growth inhibition. Natural antitubulin agents such as taxanes and Vinca alkaloids have been successful in the treatment of cancer; however, several limitations have encouraged the development of synthetic small molecule inhibitors of tubulin function. We have previously reported the discovery of two novel chemical series of tubulin polymerization inhibitors, triazoles (Ouyang et al. Synthesis and structure-activity relationships of 1,2,4-...

Regulation of N-Glycosylation by Wnt Signaling [Signal Transduction]

Journal of Biological Chemistry — Fri, 01 Oct 2010 13:39:18 +0100

In this study, we show that promoters of the first N-glycosylation gene, DPAGT1, from Chinese hamster ovary (CHO), Madin-Darby canine kidney (MDCK), and human epidermoid carcinoma (A253) cells contain the T-cell factor/lymphoid enhancer-binding factor (TCF/LEF) consensus sequence. Treatment of cells with a Wnt activator, lithium chloride, up-regulated DPAGT1 transcript levels that correlated with an increase in the β-catenin abundance. Furthermore, exposure of cells to a Wnt receptor ligand, Wnt3a, resulted in an increase in the DPAGT1 transcript levels that was abrogated by the Wnt inhibitor, Dickkopf-1. DNA mobility shift assays revealed specific protein complexes at the DPAGT1 TCF/LEF binding region that were competed off with antibodies to either Tcf3/4 or β-catenin. Chromati...

Polyamine aza-cyclic compounds demonstrate anti-proliferative activity in vitro but fail to control tumour growth in vivo.

Cell Research — Wed, 29 Sep 2010 21:55:25 +0100

Authors: Wong PE, Tetley L, DufÃ¨s C, Chooi KW, Bolton K, SchÃ¤tzlein AG, Uchegbu IF Cationic polyamines such as the poly(propylenimine) dendrimers (DAB16) are anti-tumour agents (Dufes et al., 2005, Cancer Res 65:8079-8084). Their mechanism of action is poorly understood, but the lack of in vivo toxicity suggests cancer specificity. To explore this polyamine pharmacophore we cross-linked the aza-cyclic compound, hexacyclen, with 1,4-dibromobutane or 1,8-dibromooctane to yield the polyamines [poly(butylhexacyclen)--CL4] or [poly(octylhexacyclen)--CL8] respectively, both free of primary amines. We characterised the compounds and their respective nanoparticles and examined their interaction with the putative targets of the cationic polyamines: the cell membrane and DNA. Like DAB 16, ...

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A comparative study of in-gel digestions using microwave and pressure-accelerated technologies.

Journal of Biomolecular Techniques : JBT — Tue, 31 Aug 2010 23:00:00 +0100

In this study, we compare the efficiency of several popular in-gel digestion methods, along with new technologies that may improve digestion efficiency, using a human epidermoid carcinoma cell lysate protein standard. The efficiency of each protocol was based on the average number of proteins identified and their respective sequence coverage and relative quantitation using spectral counting. The importance of this study lies in its comparison of pre-existing in-gel digestion methods with those that use newly developed technologies that may introduce the potential for a more cost-effective digestion, higher protein yield, and an overall reduction in processing time. The following four protocols were compared: an overnight in-gel digestion protocol; an overnight in-gel digestion protocol, in...

Premalignant nature of oral and vulval lichen planus: Facts and controversies

Clinics in Dermatology — Sat, 28 Aug 2010 05:43:03 +0100

Abstract: Although many classifications include oral lesions of lichen planus in the category of a premalignant condition, there is still much discussion about whether the mucous membranes lesions should be characterized as an intrinsically premalignant condition or merely as a facilitator of the action of a carcinogenic factor. The possibility that an epidermoid carcinoma can emerge at the site of lichen planus lesions, mainly in mucous membranes, has been shown; however, several published cases omit information about other potential risk factors. This prevents a complete analysis of the triggering relationship between lichen planus and squamous cell carcinoma. This contribution reviews the literature on this subject. The question of whether oral or vulval lichen planus, or both, are prem...

Effects of Bleomycin for Epidermoid Carcinoma of Head and Neck

Japanese Journal of Clinical Oncology — Tue, 24 Aug 2010 06:19:05 +0100

Bleomycin, a new antitumor agent was applied in the therapy of 100 cases of head and neck cancer. Out of the 75 cases given BLM therapy only, tumor disappeared in 10 cases and in 5 cases there was no recurrence for more than 6 months. The frequency of tumor regression was 67.5% for previously untreated cases and 42.0% for previously otherwise treated cases. Local injection of BLM were given to 5 cases of small recurrent foci which appeared after radiotherapy, of which 3 cases did not show recurrence for more than one year. Tumor regression seemed to be enhanced by simultaneous irradiation. Histological findings were often comparable to those induced by curative irradiation. They indicated a marked destruction of the tumor and occasional local cure. Eight cases of interstitial pneumonia, of...

CARCINOGENESIS FROM INHALED 239PuO2 IN BEAGLES: EVIDENCE FOR RADIATION HOMEOSTASIS AT LOW DOSES?

Health Physics — Tue, 17 Aug 2010 05:13:02 +0100

Authors: Fisher DR, Weller RE From the early 1970's to the late 1980's, Pacific Northwest National Laboratory conducted life-span studies in beagle dogs on the biological effects of inhaled plutonium (PuO2, PuO2, and Pu[NO3]4) to help predict risks associated with accidental intakes in workers. Years later, the purpose of the present follow-up study was to reassess the dose-response relationship for lung cancer in the PuO2 dogs compared to controls-with particular focus on the dose-response at relatively low lung doses. A PuO2 aerosol (2.3 mum activity-median aerodynamic diameter, 1.9 mum geometric standard deviation) was administered to six groups of 20 young (18-mo-old) beagle dogs (10 males and 10 females) by inhalation at six different activity levels, as previously described in La...

Alpha-santalol, a chemopreventive agent against skin cancer, causes G2/M cell cycle arrest in both p53-mutated human epidermoid carcinoma A431 cells and p53 wild-type human melanoma UACC-62 cells

BMC Research Notes — Mon, 02 Aug 2010 23:00:00 +0100

Conclusions: This study for the first time identifies effects of alpha-santalol in G2/M phase arrest and describes detailed mechanisms of G2/M phase arrest by this agent, which might be contributing to its overall cancer preventive efficacy in various mouse skin cancer models. (Source: BMC Research Notes)

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Salvage Reirradiaton With Stereotactic Body Radiotherapy for Locally Recurrent Head-and-Neck Tumors

International Journal of Radiation Oncology * Biology * Physics — Sun, 01 Aug 2010 23:00:00 +0100

Conclusions: Stereotactic body radiotherapy is an appealing treatment option for patients with recurrent head-and-neck cancer previously treated with radiation to high doses. Good local control with considerable 1-year survival is achieved with a relatively high rate of morbidity and related mortality. (Source: International Journal of Radiation Oncology * Biology * Physics)

Treatment of lymph nodes in epidermoid carcinoma of the penis: Review of literature by the ...

UroToday — Tue, 20 Jul 2010 23:00:00 +0100

Service d'urologie, HÃ´tel-Dieu, CHU de Nantes, 1 place Alexis-Ricordeau, Nantes, France. (Source: UroToday)

Multiplexed Evaluation of a Cell-Based Assay for the Detection of Antidrug Neutralizing Antibodies to Panitumumab in Human Serum Using Automated Fluorescent Microscopy

Journal of Biomolecular Screening — Thu, 15 Jul 2010 23:57:23 +0100

The method described here employs a high-content cell-based assay format for the detection of neutralizing antibodies (NAbs) to panitumumab, a fully human monoclonal antagonistic antibody to the human epidermal growth factor (EGF) receptor in human serum (screening assay). A specificity assay was also developed and qualified to confirm that the neutralizing activity was attributable to the presence of NAbs and not due to serum interference (serum interference assay). The ArrayScan IV HCS reader was used for the measurement of tyrosine phosphorylation of the epidermal growth factor receptor (EGFR) and STAT-1 redistribution between the cytoplasm and nucleus in the human epidermoid carcinoma cell line A431. Assay conditions were developed by (1) optimizing the response of the A431 cells to re...

Four new phenanthrenes from Monomeria barbata Lindl.

Fitoterapia — Sun, 27 Jun 2010 23:00:00 +0100

Authors: Yang M, Cai L, Tai Z, Zeng X, Ding Z Three biphenanthrene compounds (1-3) and a triphenanthrene compound (4), together with six known biphenanthrene compounds (5-10), were isolated from the tubers of Monomeria barbata Lindl. Their structures were elucidated on the basis of extensive spectroscopic analysis (1D-, 2D-NMR, and HR-ESI-MS). These four new compounds were tested in vitro for the cytotoxic activity against liver carcinoma (HepG-2), promyelocytic leukaemia (HL60), ovarian carcinoma (Skov-3) and epidermoid carcinoma (A431) cancer cell lines and the antioxidant activity in DPPH radical scavenging. Compounds 1-4 exhibited significant cytotoxic activity against HepG-2 and HL60 cell lines, and potent antioxidant activity in DPPH radical scavenging. PMID: 20600684 [PubMed...

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Bioactivity-guided isolation of antiproliferative compounds from Centaurea arenaria

Phytotherapy Research — Sun, 06 Jun 2010 23:00:00 +0100

The antiproliferative effects of n-hexane, chloroform and aqueous methanol extracts prepared from the whole plant of Centaurea arenaria M.B. ex Willd. were investigated against cervix adenocarcinoma (HeLa), breast adenocarcinoma (MCF7) and skin epidermoid carcinoma (A431) cells, using the MTT assay. The chloroform extract displayed high tumour cell proliferation inhibitory activity (higher than 85% at 10 [mgr]g/mL concentration), and was therefore subjected to a bioassay-guided multistep separation procedure. Flavonoids (eupatilin, eupatorin, 3[prime]-methyleupatorin, apigenin and isokaempferid), lignans (arctigenin, arctiin and matairesinol), the sesquiterpene cnicin, serotonin conjugates (moschamine and cis-moschamine), [beta]-amyrin and [beta]-sitosterin-[beta]-D-glycopyranoside, identi...

Synthesis and Anticancer Evaluation of Naphthoquinone Esters with 2'-Cyclopentyl and 2'-Cyclohexyl Substituents.

Bioscience, Biotechnology, and Biochemistry — Sun, 06 Jun 2010 23:00:00 +0100

Authors: Kongkathip N, Pradidphol N, Hasitapan K, Kongkathip B Twelve novel naphthoquinone esters containing cyclopentyl and cyclohexyl substituents at C-2' of the propyl chain were synthesized by starting from 1-hydroxy-2-naphthoic acid via alkylation with cyclopentyl ester and cyclohexyl ester. They were evaluated for cytotoxicity against three cancer cell lines (human epidermoid carcinoma (KB), human cervical carcinoma (HeLa), and human hepatocellular carcinoma (HepG(2))). In comparison to naphthoquinone esters with the 2',2'-dimethyl group, the naphthoquinones with a 2'-cyclopentyl substituent showed stronger activity than those with a 2'-cyclohexyl substituent, but less than that with the 2',2'-dimethyl group. This work provides new information about the effect of 2'-position subs...

11q21.1-11q23.3 Is a site of intrinsic genomic instability triggered by irradiation

Genes, Chromosomes and Cancer — Wed, 02 Jun 2010 23:00:00 +0100

The chromosome location, 11q21-23, is linked to loss of heterozygosity (LOH) in multiple tumors including those of breast, lung, and head and neck. To examine the process of LOH induction, the H292 cell line (human muco-epidermoid carcinoma) was irradiated or treated with anti-CD95 antibody, and individual clones isolated through two rounds of cloning. Regions of LOH were determined by screening a suite of eight polymorphic microsatellite markers covering 11p15-11q24 using fluorescent primers and genetic analyzer peak discrimination. LOH induction was observed extending through 11q21.1-11q23.3 in 6/49 of clones surviving 4 Gy and 8/50 after 8 Gy. Analysis of selected clones by Affymetrix 6.0 single nucleotide polymorphism (SNP) arrays confirmed the initial assessment indicating a consisten...

The distribution of the therapeutic monoclonal antibodies cetuximab and trastuzumab within solid tumors

BMC Cancer — Wed, 02 Jun 2010 23:00:00 +0100

Conclusions: Cetuximab and trastuzumab distribute slowly, but at higher doses achieve a relatively uniform distribution after about 24 hours, most likely due to their long half-lives in the circulation. There remains poor distribution within hypoxic regions of tumors. (Source: BMC Cancer)

AKAR2-AKAP12 fusion protein "biosenses" dynamic phosphorylation and localization of a GPCR-based scaffold

Journal of Molecular Signaling — Wed, 21 Apr 2010 23:00:00 +0100

Conclusion: The dynamic phosphorylation of AKAP12 "biosensed" by AKAR2-AKAP12 reveals the scaffold in association with the cell membrane, undergoing rapid phosphorylation by PKA. The perinuclear, cytoplasmic accumulation of phosphorylated scaffold reflects the phosphorylated, PKA-activated form of AKAP12, which catalyzes the resensitization and recycling of desensitized, internalized G-protein-coupled receptors. (Source: Journal of Molecular Signaling)

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In vitro cytotoxic activity of Baccharis dracunculifolia and propolis against HEp-2 cells.

Natural Product Research — Wed, 21 Apr 2010 23:00:00 +0100

Authors: Bufalo MC, Candeias JM, Sousa JP, Bastos JK, Sforcin JM Baccharis dracunculifolia is the most important vegetal source of propolis in southeast Brazil, and researchers have been investigating its biological properties. Propolis is a complex resinous hive product collected by bees from several plants, showing a very complex chemical composition. It has been employed since ancient times due to its therapeutic properties, such as antimicrobial, anti-inflammatory, antioxidant, immunomodulatory and antitumour activities, among others. The goal of this work was to compare the cytotoxic action of B. dracunculifolia, propolis and two isolated compounds (caffeic and cinnamic acids) on human laryngeal epidermoid carcinoma (HEp-2) cells in vitro. These cells were incubated with different...

Penetrating scalp Marjolin's ulcer involving bone and dura mater in a Nigerian hospital: Case report and literature review

Burns : Journal of the International Society for Burn Injuries — Tue, 13 Apr 2010 12:59:20 +0100

Marjolin's ulcers are epidermoid carcinomas developing in non-healing scar tissue . Marjolin's ulcer of the scalp is an occasional form of cutaneous cancer seen in our environment. Owing to the behaviour of the lesion and late presentation the treatment results are often disappointing. They are commonly found in the lower limbs, occasionally around the head and upper limbs, and rarely on the trunk. Marjolin's ulcers of the scalp have been variously reported , but not in the paediatric age group in this environment . (Source: Burns : Journal of the International Society for Burn Injuries)

The role of thymidine phosphorylase in the induction of early growth response protein-1 and thrombospondin-1 by 5-fluorouracil in human cancer carcinoma cells.

International Journal of Oncology — Fri, 09 Apr 2010 12:02:52 +0100

Authors: Matsushita S, Ikeda R, Nishizawa Y, Che XF, Furukawa T, Miyadera K, Tabata S, Ushiyama M, Tajitsu Y, Yamamoto M, Takeda Y, Minami K, Mataki H, Kanzaki T, Yamada K, Kanekura T, Akiyama S Thymidine phosphorylase (TP) is an enzyme involved in reversible conversion of thymidine to thymine. TP is identical to an angiogenic factor, pletelet-derived endothelial cell growth factor (PD-ECGF) and the expression levels of TP in a variety of malignant tumors were higher than the adjacent non-neoplastic tissues. To investigate the molecular basis for the effect of TP on the metabolic process and the anticancer effect of 5-fluorouracil (5-FU), human gastric carcinoma AZ521 cells and epidermoid carcinoma KB cells were transfected with TP cDNA, and AZ521/TP and KB/TP were cloned. AZ521/TP and...

Squamous cell carcinoma arising from the pleura after pneumonectomy for squamous cell carcinoma of the lung

American Journal of Surgery — Wed, 31 Mar 2010 15:12:13 +0100

Conclusions: Cancer can arise in areas of chronic inflammation such as osteomyelitis with a draining sinus, Crohn's disease, or chronic gastritis. Cases of squamous cell carcinoma arising from the pleura in patients with a chronically draining empyema cavity are extremely rare. We believe this is the sixth report in the literature of squamous cell carcinoma arising from the pleura in a patient with a chronic postpneumonectomy bronchopleural fistula. In vivo video footage of the involved pleura is available (http://video.google.com/videoplay?docid=7638947955392235731). (Source: American Journal of Surgery)

Crucial role of vinexin for keratinocyte migration in vitro and Epidermal wound healing in vivo.

Experimental Cell Research — Mon, 29 Mar 2010 23:00:00 +0100

Authors: Kioka N, Ito T, Yamashita H, Uekawa N, Umemoto T, Motoyoshi S, Imai H, Takahashi K, Watanabe H, Yamada M, Ueda K In the process of tissue injury and repair, epithelial cells rapidly migrate and form epithelial sheets. Vinexin is a cytoplasmic molecule of the integrin-containing cell adhesion complex localized at focal contacts in vitro. Here, we investigated the roles of vinexin in keratinocyte migration in vitro and wound healing in vivo. Vinexin knockdown using siRNA delayed migration of both HaCaT human keratinocytes and A431 epidermoid carcinoma cells in scratch assay, but did not affect cell proliferation. Induction of cell migration by scratching the confluent monolayer culture of these cells activated both EGFR and ERK, and their inhibitors AG1478 and U0126 substantiall...

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Inhibition of RANKL increases the anti-tumor effect of the EGFR inhibitor panitumumab in a murine model of bone metastasis

Bone — Fri, 26 Mar 2010 00:00:00 +0100

Abstract: Bone metastases cause severe skeletal complications and are associated with osteoclast-mediated bone destruction. RANKL is essential for osteoclast formation, function, and survival, and is the primary effector of tumor-induced osteoclastogenesis and osteolysis. RANKL inhibition by its soluble decoy receptor osteoprotegerin (OPG) prevents tumor-induced osteolysis and decreases skeletal tumor burden. Because osteoclast-mediated bone resorption releases growth factors from the bone matrix, the host bone microenvironment induces a vicious cycle of bone destruction and tumor proliferation and survival. A prediction of this vicious cycle hypothesis is that targeting the host bone microenvironment by osteoclast inhibition would reduce tumor growth and survival and may enhance the anti-...

Synthesis and in-vitro Cytotoxicity of Poly-functionalized 4-(2-Arylthiazol-4-yl)-4H-chromenes

Archiv der Pharmazie — Mon, 22 Mar 2010 00:00:00 +0100

A new series of 4-aryl-4H-chromenes bearing a 2-arylthiazol-4-yl moiety at the 4-position were prepared as potential cytotoxic agents. The in-vitro cytotoxic activity of the synthesized 4-aryl-4H-chromenes was investigated in comparison with etoposide, a well-known anticancer drug, using MTT colorimetric assay. Among them, the 2-(2-chlorophenyl)thiazol-4-yl analog 4b showed the most potent activity against nasopharyngeal epidermoid carcinoma KB, medulloblastoma DAOY, and astrocytoma 1321N1, and compound 4d bearing a 2-(4-chlorophenyl)thiazol-4-yl moiety at the 4-position of the chromene ring exhibited the best inhibitory activity against breast cancer cells MCF-7, lung cancer cells A549, and colon adenocarcinoma cells SW480 with IC50 values less than 5 [mu]M. The ability of compound 4b to ...

Effectiveness of modified radical neck dissection and postoperative radiotherapy.

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Fri, 05 Mar 2010 00:00:00 +0100

DISCUSSION: MRND in association with PORT is an effective treatment in patients with advanced head and neck epidermoid carcinoma staged N2 and N3. PMID: 20207384 [PubMed - as supplied by publisher] (Source: Revue de Stomatologie et de Chirurgie Maxillo-Faciale)

Andrographolide regulates epidermal growth factor receptor and transferrin receptor trafficking in epidermoid carcinoma (A-431) cells

British Journal of Pharmacology — Wed, 03 Mar 2010 00:00:00 +0100

Conclusion and implications: This study sheds light on how andrographolide may affect receptor trafficking by inhibiting receptor movement from the late endosomes to lysosomes. The down-regulation of EGFR from the cell surface also indicates a new mechanism by which andrographolide may induce cancer cell death. (Source: British Journal of Pharmacology)

A Review of Penile Cancer

Advances in Urology — Tue, 16 Feb 2010 15:49:20 +0100

Conclusion. Penile carcinoma is a rare pathology which affects elderly persons and is diagnosed late. (Source: Advances in Urology)

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gamma-Glutamyl 16-diaminopropane derivative of vasoactive intestinal peptide: a potent anti-oxidative agent for human epidermoid cancer cells.

Amino Acids — Wed, 10 Feb 2010 00:00:00 +0100

Authors: Stiuso P, Giuberti G, Lombardi A, Dicitore A, Limongelli V, Cartenì M, Abbruzzese A, Caraglia M We previously demonstrated that the gamma-glutamyl 16 amine derivative of vasoactive intestinal peptide (VIP) acts as structural VIP agonist with affinity and potency higher than VIP. Herein, we have evaluated the effects of VIP and gamma-Gln16-diaminopropane derivative of VIP (VIP-DAP3) on the proliferation and protection from oxidative stress induced by hydrogen peroxide (H(2)O(2)) on epidermoid carcinoma cell lines. We have found that 10(-11) M VIP-DAP3 completely antagonized the inhibition induced by H(2)O(2) on both cell proliferation and S-phase distribution while these effects were only partially antagonized by equimolar concentrations of VIP. Moreover, both oxidative st...

Complex tracheal lesion: correction with an intercostal muscle pedicle flap

Jornal Brasileiro de Pneumologia — Sat, 30 Jan 2010 15:33:10 +0100

We report the case of a patient diagnosed with epidermoid carcinoma in the distal third of the esophagus. The patient was submitted to esophagectomy by video-assisted thoracoscopy and laparoscopy. During the operation, the left main bronchus was injured, and this required immediate surgical correction. In the postoperative period, the patient presented with acute respiratory failure and profuse air leak through the thoracic drains and through the cervical surgical wound. The patient underwent a second surgical procedure, during which a large lesion was discovered in the membranous wall of the trachea. The lesion was corrected with an intercostal muscle pedicle flap. (Source: Jornal Brasileiro de Pneumologia)

Integrative molecular characterization of head and neck cancer cell model genomes

Head and Neck — Wed, 16 Dec 2009 00:00:00 +0100

Cell lines are invaluable model systems for the investigation of cancer. Knowledge of the molecular alterations that exist within cell models is required to define the mechanisms governing cellular phenotypes.Five tongue squamous cell carcinomas cell lines and 1 submaxillary salivary gland epidermoid carcinoma cell line were analyzed for copy number and mRNA expression by tiling-path DNA microarrays and Agilent Whole Human Genome Oligoarrays, respectively.Integrative analysis of genetic and expression alterations revealed the molecular landscape of each cell line. Molecular results for individual cell lines and across all samples have been summarized and made available for easy reference.Our integrative genomic analyses have defined the DNA and RNA alterations for each individual line. The...

In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells

Evidence-based Complementary and Alternative Medicine — Tue, 24 Nov 2009 15:14:55 +0100

Propolis is a sticky dark-colored material showing a very complex chemical composition that honeybees collect from plants. It has been used in folk medicine since ancient times, due to several biological properties, such as antimicrobial, anti-inflammatory, antioxidant and immunomodulatory activities, among others. Its antitumor action in vivo and in vitro has also been reported, using propolis extracts or its isolated compounds. The goal of this work was to evaluate propolis's cytotoxic action in vitro on human laryngeal epidermoid carcinoma (Hep-2) cells. These cells were incubated with different concentrations of this bee product for different time periods, and morphology and the number of viable HEp-2 cells analyzed. Data showed that propolis exhibited a cytotoxic effect in vitro again...

Identification of MYO18A as a Novel Interacting Partner of the PAK2/{beta}PIX/GIT1 Complex and Its Potential Function in Modulating Epithelial Cell Migration.

Mol Biol Cell — Wed, 18 Nov 2009 00:00:00 +0100

Authors: Hsu RM, Tsai MH, Hsieh YJ, Lyu PC, Yu JS Monitoring Editor: Jonathan Chernoff The p21-activated kinase (PAK) 2 is known to be involved in numerous biological functions, including the regulation of actin reorganization and cell motility. To better understand the mechanisms underlying this regulation, we herein used a proteomic approach to identify PAK2-interacting proteins in human epidermoid carcinoma A431 cells. We found that MYO18A, an emerging member of the myosin superfamily, is a novel PAK2 binding partner. Using a siRNA knockdown strategy and in vitro binding assay, we discovered that MYO18A binds to PAK2 through the betaPIX/GIT1 complex. Under normal conditions, MYO18A and PAK2 colocalized in lamellipodia and membrane ruffles. Interestingly, knockdown of MYO18A in cells...

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Impact of target volumes and radiation technique on loco-regional control and survival for patients with unilateral cervical lymph node metastases from an unknown primary

Radiotherapy and Oncology — Fri, 06 Nov 2009 00:00:00 +0100

Conclusions: Retrospective comparisons between bilateral and unilateral neck radiotherapies did not show differences in terms of loco-regional control and survival. However, patient’s local regional control and survival are significantly improved after 3D-CRT or IMRT. (Source: Radiotherapy and Oncology)

Impact of target volumes and radiation technique on loco-regional control and survival for patients with unilateral cervical lymph node metastases from an unknown primary.

Wed, 04 Nov 2009 00:00:00 +0100

CONCLUSIONS: Retrospective comparisons between bilateral and unilateral neck radiotherapies did not show differences in terms of loco-regional control and survival. However, patient's local regional control and survival are significantly improved after 3D-CRT or IMRT. PMID: 19892420 [PubMed - as supplied by publisher] (Source: Radiotherapy and Oncology : journal of the European Society for Therapeutic Radiology and Oncology)

[Definitive treatment of anal canal carcinoma with radiotherapy: Adverse impact of a pre-radiation resection. A retrospective study of 57 patients treated with curative intent.]

Cancer Radiotherapie — Thu, 22 Oct 2009 00:00:00 +0100

CONCLUSION: Radiotherapy and chemoradiation with cisplatine-based chemotherapy cure a vast majority of patients with epidermoid carcinoma of the anal canal. Therapeutic factors that may interfere with the definition of the target volume and the patients' repositioning may decrease the efficacy of radiotherapy. Pre-radiotherapy surgical resection should be avoided. PMID: 19854092 [PubMed - as supplied by publisher] (Source: Cancer Radiotherapie)

[TNF-alpha inhibitor etanercept and oral cavity carcinoma.]

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Tue, 13 Oct 2009 23:00:00 +0100

DISCUSSION: The possible development of an oral cavity carcinoma should be taken into account when following a patient under TNF-alpha inhibitor treatment. Anti-TNF treatment has improved the management of patients with severe chronic inflammatory diseases. They allow for a better quality of life. Nevertheless, their immunosuppressive effect should be taken into account when prescribed and during follow-up. PMID: 19836037 [PubMed - as supplied by publisher] (Source: Revue de Stomatologie et de Chirurgie Maxillo-Faciale)

Essential Oil of Artemisia Capillaris Induces Apoptosis in KB Cells via Mitochondrial Stress and Caspase Activation Mediated by MAPK-Stimulated Signaling Pathway

Journal of Food Science — Mon, 12 Oct 2009 23:00:00 +0100

This study examined whether or not the essential oil isolated from A. capillaris induces apoptosis in the human oral epidermoid carcinoma cells along with the possible mechanism(s) of the essential oil-mediated cytotoxicity. The essential oil induced cell death of KB cells through apoptosis, as evidenced by the increased cell population in the sub-G1 phase, the appearance of condensed and/or fragmented nuclei, and the generation of a cleaved PARP product. Treating the cells with the oil also caused changes in the mitochondrial level of the Bcl-2 family proteins such as Bcl-2 and Bax, thereby inducing the release of cytochrome c into the cytosol. Additional experiments using caspase or mitogen-activated protein kinase inhibitors suggested the involvement of the p38/NF-[kappa]B and JNK/Bcl-2...

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Is frozen section reliable in transoral CO2 laser-assisted cordectomies?

European Archives of Oto-Rhino-Laryngology — Tue, 29 Sep 2009 04:04:53 +0100

Abstract Endoscopic resection of laryngeal tumors is replacing external approaches. One drawback of endoscopic resection is the difficulty of interpretation of histological specimens because of thermal effect of laser on tissues. Our goal is to assess the reliability of frozen section in this setting by comparing its results with those of routine histology. We, retrospectively, reviewed the charts of all consecutive patients, who underwent cordectomies in our institution between January 2000 and 2008, using the CO2 laser Acublade system (Lumenis, Santa Clara, CA). Age, sex, staging of the tumor, previous treatments, type of cordectomy, frozen section and routine histology results were analyzed. Ninety-seven patients fulfilled the inclusion criteria; 22.7% had severe dyspla...

[11C]Gefitinib ([11C]Iressa): Radiosynthesis, In Vitro Uptake, and In Vivo Imaging of Intact Murine Fibrosarcoma

Molecular Imaging and Biology — Tue, 29 Sep 2009 01:05:47 +0100

Conclusion These results demonstrated the promising potential of [11C]gefitinib to serve as a PET ligand for in vivo imaging of NFSa-bearing mice. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0265-5Authors Ming-Rong Zhang, National Institute of Radiological Sciences Department of Molecular Probes, Molecular Imaging Center 4-9-1 Anagawa, Inage-ku Chiba JapanKatsushi Kumata, National Institute of Radiological Sciences Department of Molecular Probes, Molecular Imaging Center 4-9-1 Anagawa, Inage-ku Chiba JapanAkiko Hatori, National Institute of Radiological Sciences Department of Molecular Probes, Molecular Imaging Center 4-9-1 Anagawa, Inage-ku Chiba JapanNobuhiko Takai, National Institute of Radiological Sciences Heavy-Ion Radiobiology Rese...

EGCG induces apoptosis in human laryngeal epidermoid carcinoma Hep2 cells via mitochondria with the release of apoptosis-inducing factor and endonuclease G

Cancer Letters — Fri, 25 Sep 2009 00:00:00 +0100

Abstract: (−)-Epigallocatechin-3-gallate (EGCG), a major green tea polyphenol, was tested for in vitro cytotoxicity against human laryngeal epidermoid carcinoma of the larynx Hep2 cells. EGCG-induced apoptotic cell death accompanied by a change in the cell cycle. However, EGCG did not result in caspase activation, nor did a caspase inhibitor block cell death. Furthermore, EGCG caused no change in the intracellular levels of reactive oxygen species (ROS). The levels of p53 were increased in the EGCG-treated cells, with a corresponding decrease in Bcl-2 and Bid protein levels as well as an increase in the Bax level. In addition, EGCG induced the cytoplasmic release of cytochrome c from the mitochondria accompanied by a decreased mitochondrial membrane potential, and subsequently upregulate...

Identification and Characterization of Lutheran Blood Group Glycoprotein as a New Substrate of Membrane-type 1 Matrix Metalloproteinase 1 (MT1-MMP): A SYSTEMIC WHOLE CELL ANALYSIS OF MT1-MMP-ASSOCIATING PROTEINS IN A431 CELLS [Enzyme Catalysis and Regulation]

Journal of Biological Chemistry — Thu, 24 Sep 2009 23:00:00 +0100

Membrane-type 1 matrix metalloproteinase 1 (MT1-MMP) is a potent modulator of the pericellular microenvironment and regulates cellular functions in physiological and pathological settings in mammals. MT1-MMP mediates its biological effects through cleavage of specific substrate proteins. However, our knowledge of MT1-MMP substrates remains limited. To identify new substrates of MT1-MMP, we purified proteins associating with MT1-MMP in human epidermoid carcinoma A431 cells and analyzed them by mass spectrometry. We identified 163 proteins, including membrane proteins, cytoplasmic proteins, and functionally unknown proteins. Sixty-four membrane proteins were identified, and they included known MT1-MMP substrates. Of these, eighteen membrane proteins were selected, and we confirmed their asso...

Termination of tyrphostin AG1478 application results in different recovery of EGF receptor tyrosine residues 1045 and 1173 phosphorylation in A431 cells.

Cell Biology International — Fri, 18 Sep 2009 00:00:00 +0100

Authors: Kondratov KA, Chernorudskiy AL, Amosova AP, Kornilova ES Tyrphostin AG1478 is known as a specific and reversible inhibitor of tyrosine kinase (TK) activity of the EGF receptor (EGFR). It is attractive as an anti-cancer agent for cancers with elevated EGFR TK levels. However, post-application effects of AG1478 are not well studied. We have analyzed EGFR phosphorylation after termination of AG1478 application using human epidermoid carcinoma A431 cells. It was found that AG1478 inhibitory action is fast but not fully reversible: removal of tyrphostin resulted in incomplete restoration of the overall EGFR phosphorylation. Analyzing the state of two individual autophosphorylation sites of internalized EGFR, Y1045 and Y1173, we demonstrated that phosphorylation of Y1173 involved in...

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Reconstruction of the pulmonary artery for lung cancer: Long-term results

The Journal of Thoracic and Cardiovascular Surgery — Wed, 09 Sep 2009 23:00:00 +0100

Conclusions: Pulmonary artery reconstruction is safe, with excellent long-term survival. Our results support this technique as an effective option for patients with lung cancer. (Source: The Journal of Thoracic and Cardiovascular Surgery)

[6]-Gingerol induces reactive oxygen species regulated mitochondrial cell death pathway in human epidermoid carcinoma A431 cells.

Chemico-Biological Interactions — Wed, 19 Aug 2009 22:34:25 +0100

Authors: Nigam N, Bhui K, Prasad S, George J, Shukla Y Since skin cancer incidence and prevalence is constantly rising up the charts despite all efforts, search for newer, better agents for protection and treatment is required. Ginger (Zingiber officinale Roscoe), a monocotyledonous herb, is widely used as a herbal medicine, given the presence of homologous phenolic ketones, of which [6]-gingerol is the major one. The quantity of [6]-gingerol in the fresh ginger rhizome was found to be 104-965 microg/g in common varieties of ginger available in Indian market. Herein, [6]-gingerol was assessed for its anti-apoptotic effects in human epidermoid carcinoma A431 cells. [6]-Gingerol treatment exhibited considerable cytotoxicity as indicated by growth inhibition of A431 cells mediated via gen...

Immunohistochemical expression of ubiquitin and telomerase in cervical cancer

Virchows Archiv — Wed, 19 Aug 2009 08:30:12 +0100

Abstract Ubiquitin and telomerase immunohistochemical expression patterns in cervical cancer were compared with normal cervical tissue samples. Eighty-one cervical cancer cases and 22 normal exo–endocervical tissue were examined with polyclonal antibody for ubiquitin and 44G12 clone for telomerase using tissue microarrays. The results were interpreted using a semiquantitative scale The average age of patients was 50.67 years. The most frequent histological types were moderately differentiated epidermoid carcinoma (43.5%), according to the degree of differentiation, and endocervical adenocarcinoma (42.1%). Immunohistochemical findings were as follows: 98.7% of cervical cancers showed immunoexpression for ubiquitin and 52.6% for telomerase. Statistically significant d...

Reproducibility of the kinetic analysis of 3′-deoxy-3′-[18F]fluorothymidine positron emission tomography in mouse tumor models

Nuclear Medicine and Biology — Wed, 29 Jul 2009 23:00:00 +0100

Abstract: Objectives: We assessed the reproducibility of the kinetic analysis of 3′-deoxy-3′-[18F]fluorothymidine (FLT) positron emission tomography (PET) in A431 human epidermoid carcinoma and murine Lewis lung carcinoma (LLC) tumor models.Methods: We injected 7.4 MBq of FLT (n=10 for each group) and acquired 2-h dynamic PET images. A second scan was performed 1 day later. We calculated standardized uptake value (SUV), kinetic rate constants, volume of distribution of phosphorylated FLT (Vdm), net influx constant (KFLT-CA) and influx constant by Patlak graphical analysis (KFLT-PA). The percent difference between measurements of a parameter was calculated to compare the reproducibilities of different parameters.Results: FLT phosphorylation was higher in mice with A431 tumors than in mi...

Bioactivity-Guided Isolation of Cytotoxic Sesquiterpenes and Flavonoids from Anthemis ruthenica

Planta Medica — Wed, 29 Jul 2009 10:52:38 +0100

Planta MedDOI: 10.1055/s-0029-1185942AbstractA new eudesmanolide sesquiterpene, sivasinolide 6--angelate (), was isolated from the aerial parts of together with the known compounds chrysanin (), tanacin (), 3-hydroxycostunolide (), centauridin (), and centaureidin (). The compounds were obtained by means of bioactivity-guided fractionation from the CHCl extract of the herb, which displayed high cytotoxic activity. The structures were determined by UV, HR‐ESI‐MS, and high-field 1D and 2D NMR spectral analyses, affording complete H- and C‐NMR assignments for all compounds. The cytotoxic activities of the isolated sesquiterpenes and flavonoids were assessed against cervical adenocarcinoma HeLa, breast adenocarcinoma MCF7, and skin epidermoid carcinoma A431 cells using ...

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Up-regulation of the tight-junction protein ZO-1 by substance P and IGF-1 in A431 cells

Cell Biochemistry and Function — Mon, 27 Jul 2009 23:00:00 +0100

The formation of a barrier by tight junctions is important in epithelia of various tissues. Substance P (SP) and insulin-like growth factor (IGF)-1 synergistically promote barrier function in the corneal epithelium. We have now examined the effects of SP and IGF-1 on expression of the tight-junction protein zonula occludens (ZO)-1 in A431 human epidermoid carcinoma cells. Reverse transcription-polymerase chain reaction (RT-PCR) and immunoblot analyses revealed that SP and IGF-1 increased the amounts of ZO-1 mRNA and protein in these cells in a concentration-dependent manner, with neither SP nor IGF-1 alone having such an effect. The SP- and IGF-1-induced up-regulation of ZO-1 was accompanied by phosphorylation of extracellular signal-regulated kinase (ERK), and both of these effects were b...

Overexpression of DPAGT1 Leads to Aberrant N-Glycosylation of E-Cadherin and Cellular Discohesion in Oral Cancer

Cancer Research — Tue, 14 Jul 2009 23:00:00 +0100

Cancer cells are frequently characterized by aberrant increases in protein N-glycosylation and by disruption of E-cadherin–mediated adherens junctions. The relationship between altered N-glycosylation and loss of E-cadherin adhesion in cancer, however, remains unclear. Previously, we reported that complex N-glycans on the extracellular domains of E-cadherin inhibited the formation of mature adherens junctions. Here, we examined whether dysregulated N-glycosylation was one of the underlying causes for cellular discohesion in oral cancer. We show that dense cultures of human salivary epidermoid carcinoma A253 cells exhibited elevated expression of DPAGT1, the gene that initiates protein N-glycosylation. Overexpression of DPAGT1 correlated with the production of E-cadherin–bearing...

Pyripyropenes, Fungal Sesquiterpenes Conjugated with alpha-Pyrone and Pyridine Moieties, Exhibits Anti-angiogenic Activity against Human Umbilical Vein Endothelial Cells.

Biological and Pharmaceutical Bulletin — Tue, 30 Jun 2009 23:00:00 +0100

Authors: Hayashi A, Arai M, Fujita M, Kobayashi M In the course of our search for anti-angiogenic substances, pyripyropenes A (1), B (2), and D (3) were re-discovered as selective anti-proliferative substances against human umbilical vein endothelial cells (HUVECs) from a marine-derived fungus of Aspergillus sp. Pyripyropenes showed potent anti-proliferative activity against HUVECs with IC(50) values of the range of 0.1-1.8 muM, which were cytostatic at 0.05 to 20 muM. The selective index was more than 55-fold in comparison with those of several tumor cell lines. Compound 1 inhibited vascular endothelial growth factor (VEGF)-induced migration and tubular formation of HUVECs, while 1 showed no effect on the VEGF-induced phosphorylations of extracellular signal-regulated kinase (ERK)1/2,...

Inhibition of 12-LOX and COX-2 reduces the proliferation of human epidermoid carcinoma cells (A431) by modulating the ERK and PI3K-Akt signalling pathways

Experimental Dermatology — Tue, 23 Jun 2009 23:00:00 +0100

Please cite this paper as: Inhibition of 12-LOX and COX-2 reduces the proliferation of human epidermoid carcinoma cells (A431) by modulating the ERK and PI3K-Akt signalling pathways. Experimental Dermatology 2009.Abstract: Eicosanoids, the oxygenated metabolites of arachidonic acid (AA), mediate a variety of human diseases, such as cancer, inflammation and arthritis. To evaluate the role of eicosanoids in epidermoid carcinoma, the expression of AA metabolizing enzymes, such as lipoxygenases (LOXs) and cyclooxygenases (COXs), was analysed in a human epidermoid carcinoma cell line (A431). These studies revealed overexpression of 12-R-LOX and COX-2 in A431 cells. Baicalein (a 12-LOX inhibitor) and celecoxib (a COX-2 inhibitor) significantly reduced thymidine incorporation, whereas 12-(R)-HETE...

Metastatic Liposarcoma to the Parotid

Journal of Oncology — Tue, 09 Jun 2009 15:11:50 +0100

We report an exceptional case of parotid metastasis of myxoid liposarcoma in a 42-year-old man and insist on the worse prognosis of this entity. (Source: Journal of Oncology)

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A dramatic case of genital radionecrosis

International Urology and Nephrology — Sun, 31 May 2009 08:22:43 +0100

Abstract Background Epidermoid carcinoma of the penis presents in 1% of malignant tumors in men. Penis cancer treatment has traditionally been total or partial amputation. There are authors who consider radiation to be part of penis cancer treatment in select cases—especially the use of brachytherapy and external radiation. Clinical case The case of a 48-year-old male who in 2004 presented with an ulcerated lesion of the penis that was left unattended to is presented. Two years later, he sought medical attention and physical examination revealed an ulcerated penile lesion with poorly defined edges and limited to the glans. Diagnosis The lesion was biopsied and the histopathological study reported Epidermoid Carcinoma. After radiological...

Anal Squamous Cell Carcinoma in the HIV-Positive Patient

Clinics in Colon and Rectal Surgery — Thu, 28 May 2009 08:29:49 +0100

This article describes the disease process and current treatment options.[...]© Thieme Medical PublishersGet connected:Table of contents | Abstract | Full text (Source: Clinics in Colon and Rectal Surgery)

Breast cancer resistant protein (BCRP) is a molecular determinant of the outcome of photodynamic therapy (PDT) for centrally located early lung cancer

Lung Cancer — Mon, 25 May 2009 00:00:00 +0100

Abstract: The ATP-binding cassette (ABC) transporter protein, BCRP (breast cancer resistance protein)/ABCG2 pumps out some types of photosensitizers used in photodynamic therapy (PDT) and causes resistance to the antitumor effect of PDT. The purpose of this study was to investigate the association between the expression of BCRP and the efficacy of PDT using Photofrin, or the second-generation photosensitizer, NPe6, for centrally located early lung cancers.Using human epidermoid carcinoma cells, A431 cells and the BCRP-overexpressing A431/BCRP cells, we examined the effects of BCRP expression on the effect of PDT by cell viability assay in vitro, and investigated the expression of BCRP by immunohistochemical analysis in 81 tumor samples obtained from patients with centrally located early lu...

Malignant tumours development in peri-implant tissues in oncologic and non-oncologic patients: a report of two cases

International Journal of Oral and Maxillofacial Surgery — Fri, 01 May 2009 04:00:00 +0100

We report two cases of squamous cell carcinoma arising in peri-implant tissues after dental implant placement. A 35-year-old woman was referred to us one month after prosthetic rehabilitation following insertion of an implant in the area 36 four months earlier. She presented with a peri-implant tissue lesion with appearance of inflammatory tissue. Its biopsy was reported as infiltrate epidermoid carcinoma. Second case is a 75-year-old male with resection of epidermoid carcinoma affecting the lower left gingiva and mandible and reconstruction with iliac crest free flap fourteen years ago. Further fixed rehabilitation with osseo-integrated implants was carried out after reconstruction. Second primary malignancy in the tissue around one of the implant was developed. In both cases evolution wa...

A new acyclic diterpene acid and bioactive compounds from Knema glauca.

Archives of Pharmacal Research — Thu, 30 Apr 2009 23:00:00 +0100

Authors: Rangkaew N, Suttisri R, Moriyasu M, Kawanishi K Investigation of the chemical constituents of the fruits of Knema glauca (Myristicaceae) yielded a new acyclic diterpene acid, named glaucaic acid 4, together with four acylphenols, including 1-(2,6-dihydroxyphenyl) tetradecan-1-one 1, malabaricone A 6, dodecanoylphloroglucinol 7 and 1-(2,4,6-trihydroxyphenyl)-9-phenylnonan-1-one 8, two lignans sesamin 2 and asarinin 3, and a flavan, myristinin D 5. In addition, myristinin A 9 and (+/-)-7,4'-dihydroxy-3'-methoxyflavan 10 were isolated from its leaves and stems, respectively. When tested against small-cell lung cancer (NCI-H187), epidermoid carcinoma (KB) and breast cancer (BC) cell lines, compounds 1, 6-8 and 10 displayed weak to moderate cytotoxicity. The acylphenols 6-8 display...

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Bee Propolis Stops Tumors from Neurofibromatosis and Cancer

NaturalNews.com — Wed, 29 Apr 2009 07:00:00 +0100

(NaturalNews) Honeybees are some of the most amazing creatures ever created by nature. They play a vital role in much of the food supply through pollination, and they provide people with a healthy natural alternative to sugar. Some of the most interesting aspects of bees are the substances they make to use themselves. Propolis is a natural resin found in young tree buds. Bees collect it for use as a glue to seal their homes. Propolis is an exceedingly complex product that contains substances that prevent and treat diseases ranging from cancer to the common cold. Recent research has documented the ability of propolis to suppress the growth of human tumors from neurofibromatosis and cancer.Compound from propolis halts tumor process in clinical trialsNeurofibromatosis is a genetic disorder th...

[Carcinoma of the lips.]

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Tue, 07 Apr 2009 23:00:00 +0100

Authors: Ben Slama L Epidermoid carcinoma, that is, squamous cell carcinoma of the skin, is the most common malignant tumor of the lips. It occurs especially in men. Its primary causes are sun exposure, smoking, and chronic irritation. Leukoplakia is the most frequent precancerous lesion. Epidermoid carcinoma may appear clinically as a scaly erosion or an ulceration. Standard treatment is surgical excision with reconstruction. PMID: 19361830 [PubMed - as supplied by publisher] (Source: Revue de Stomatologie et de Chirurgie Maxillo-Faciale)

Two New Sesquiterpenoids from Solanum lyratum with Cytotoxic Activities.

Chemical and Pharmaceutical Bulletin — Wed, 01 Apr 2009 04:00:00 +0100

Authors: Ren Y, Shen L, Zhang DW, Dai SJ Two new sesquiterpenoids, lyratol C (1) and lyratol D (2), together with two known sesquiterpenoids, dehydrovomifoliol (3) and blumenol A (4), were isolated from the whole plant of Solanum lyratum. Their structures were established by spectroscopic analyses. In vitro, the four compounds showed significant cytotoxic activities against three human cancer cell lines, namely, HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells, and gave IC(50) values in the range 3.7-8.1 muM. PMID: 19336938 [PubMed - in process] (Source: Chemical and Pharmaceutical Bulletin)

Ganoderma tsugae extract inhibits expression of epidermal growth factor receptor and angiogenesis in human epidermoid carcinoma cells: In vitro and in vivo.

Cancer Letters — Sat, 28 Mar 2009 04:00:00 +0100

We examined the anti-angiogenic effects of Ganoderma tsugae methanol extract (GTME) on human epidermoid carcinoma A-431 cells. Our data indicate that GTME inhibits the expression of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) in vitro and in vivo, and also inhibits the capillary tube formation of human umbilical vein endothelial cells (HUVECs). We also show that the suppression of VEGF expression by GTME can be restored by treatment with EGF. These results suggest that GTME inhibits VEGF expression via the suppression of EGFR expression, resulting in the downregulation of VEGF secretion from epidermoid carcinoma A-431 cells. These findings reveal a novel role for G. tsugae in inhibiting EGFR and VEGF expression, which are important for tumor angiog...

Molecular Imaging and Quantitative Measurement of Epidermal Growth Factor Receptor Expression in Live Cancer Cells Using Immunolabeled Gold Nanoparticles

American Journal of Roentgenology — Thu, 19 Mar 2009 04:00:00 +0100

CONCLUSION. Immunolabeled nanoparticles can quantify receptor expression with performance comparable to fluorescence markers and show promise to better characterize receptor expression via their refractive index sensitivity. (Source: American Journal of Roentgenology)

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Identification and characterization of a novel CXC chemokine in xenograft tumor induced by mas-overexpressing cells

International Journal of Cancer — Thu, 19 Mar 2009 00:00:00 +0100

Overexpressions of G protein-coupled receptor (GPCR) with elevated downstream signaling events have been reported in various tumors. However, the cellular mechanism that GPCR overexpression leads to tumor formation is largely unknown. The orphan GPCR mas was originally isolated from a human epidermoid carcinoma. In vivo studies of mas-overexpressing cells suggested that xenograft tumor formation was positively correlated with the levels of mas expression. Histochemical analysis indicated that xenograft tumor consisted of mas-transfected and stromal cells. Biochemical analyses revealed that cells overexpressing mas exhibited significantly increased anchorage-independent growth, whereas there was no significant difference in cell proliferation in comparison with empty vector-transfected cont...

A rare case of invasive vaginal carcinoma associated with vaginal prolapse

Archives of Gynecology and Obstetrics — Thu, 05 Mar 2009 14:59:36 +0100

Conclusions Careful preoperative histological evaluation of cervix in cases of uterine prolapse and postoperative cytologic and colposcopic follow-up of the vagina after hysterectomies are important because possible association with cervical carcinoma and occurrence of late vaginal cancer. Surgery and radiotherapy were effectively combined in this case that remains with no recurrence after 2 years follow-up. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s00404-009-1016-1Authors Thales Paulo Batista, University of Pernambuco Centro de Oncologia, Hospital Universitário Oswaldo Cruz Recife PE BrazilJosé Alves Morais, University of Pernambuco Centro de Oncologia, Hospital Universitário Oswaldo Cruz Recife PE BrazilTarcísio José Cysneiros Costa Rei...

Radiotherapy for Epidermoid Carcinoma of the Anus: Thirty Years' Experience

International Journal of Radiation Oncology * Biology * Physics — Mon, 02 Mar 2009 00:00:00 +0100

Conclusion: Our results have confirmed that CRT is an effective approach. Patients with human immunodeficiency virus can be treated with CRT. Tumor mobility significantly predicts the outcome; the implications for management are discussed. We also discuss the treatment planning implications of the late morbidity findings. The substantial incidence of additional malignancies underscores the importance of full oncologic screening during follow-up. (Source: International Journal of Radiation Oncology * Biology * Physics)

Sarcophine-diol, a Chemopreventive Agent of Skin Cancer, Inhibits Cell Growth and Induces Apoptosis through Extrinsic Pathway in Human Epidermoid Carcinoma A431 Cells.

Translational Oncology — Sun, 01 Mar 2009 05:00:00 +0100

Authors: Zhang X, Bommareddy A, Chen W, Khalifa S, Kaushik RS, Fahmy H, Dwivedi C Sarcophine-diol (SD), a structural modifications of sarcophine, has shown chemopreventive effects on 7,12-dimethylbenz(a)anthracene-initiated and 12-O-tetradecanoylphorbol-13-acetate-promoted skin tumor developments in mice. Tumorigenesis is associated with uncontrolled cell growth and loss of apoptosis. In the present study, the effects of SD on cell growth and apoptosis in human epidermoid carcinoma A431 cells were determined to assess whether SD could inhibit cell growth and/or induce apoptosis, thus elucidating possible mechanism of action. MTT assay was used for cell viability; bromodeoxyuridine incorporation assay was used for cell proliferation; fluorescence-activated cell sorting analysis of annex...

[Tumoral pathology of salivary glands. Our experience]

Acta Otorrinolaringologica Espanola — Sun, 01 Mar 2009 00:00:00 +0100

CONCLUSIONS: We have found two main differences with previously reported studies: the elevated mean age of patients with malignant tumours and, probably as a result of this advanced age, the high incidence of squamous cell carcinoma due to metastatic dissemination of skin cancers. PMID: 19401079 [PubMed - in process] (Source: Acta Otorrinolaringologica Espanola)

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[Adult Schönlein-Henoch purpura associated with epidermoid carcinoma of the lung.]

Nephrologie & Therapeutique — Thu, 12 Feb 2009 05:00:00 +0100

CONCLUSION: The neoplasm could be responsible of the development of the Schönlein-Henoch purpura. The discovery of this systemic vasculitis in an elderly patient should warrant a deep screening for an occult neoplasm. PMID: 19217841 [PubMed - as supplied by publisher] (Source: Nephrologie & Therapeutique)

[Isolated mandibular B-cell lymphoma revealed by inferior alveolar nerve anesthesia.]

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Mon, 02 Feb 2009 05:00:00 +0100

DISCUSSION: Our observation is unusual in its clinical presentation. Mandibular lymphomas most often present as a painless swelling, sometimes ulcerated in the mouth. They are very rarely diagnosed after an isolated hypoesthesia of V3. Lymphomas are the second most frequent head and neck lymphomas after epidermoid carcinomas, but the frequency seems to be increasing. In almost all the cases, they present as B-cell tumours of the DLBCL subtype in the WHO classification. Mandibular localizations account for only 0.6% of the cases. They are often misdiagnosed as a dental problem. The complete remission rate after chemotherapy ranges from 60 to 80% at one year. Nevertheless, the prognosis remains bad with a survival rate of only 50% at five years. PMID: 19193387 [PubMed - as supplied by pu...

Antiproliferative activity of Hungarian Asteraceae species against human cancer cell lines. Part II

Phytotherapy Research — Fri, 23 Jan 2009 05:00:00 +0100

The antiproliferative activities of aqueous and organic extracts prepared from 26 Hungarian species of the tribes Cynereae and Lactuceae (Asteraceae) were tested in vitro against HeLa (cervix epithelial adenocarcinoma), A431 (skin epidermoid carcinoma) and MCF7 (breast epithelial adenocarcinoma) cells by using the MTT assay. Of the tested 200 extracts of different plant parts obtained with n-hexane, chloroform, 50% methanol and water, 16 extracts displayed noteworthy cell growth inhibitory activity (>50% inhibition at a concentration of 10 µg/mL). The IC50 values of these extracts were determined, and their direct cytotoxic effects were measured. High differences between the antiproliferative and cytotoxic activities, demonstrating a real cell proliferation inhibitory activity rather than...

The histopathological typing of 48 cases of ESO-phageal adenocarcinoma and its tissue origin

Chinese Journal of Cancer Research — Sat, 03 Jan 2009 06:42:00 +0100

Abstract Of 2487 cases of esophageal carcinoma pathologically confirmed after resection in 1978–1988 in Shantou, adenocarcinoma accounted for 1.93% (48 cases). These were divided into 6 types; Ordinary well - differentiated adenocarcinoma, 10 cases; Ordinary poorly - differentiated adenocarcinoma, 11 cases; Adeno - squamous carcinoma, 18 cases; Muco - epidermoid carcinoma, 4 cases; “Basal Cell - Like Carcinoma”, 4 cases; Cylindroma - Like carcinoma, 1 case. Mucohistochemistry and immuno-histochemistry studies revealed some differences among these types; And their prognosis also varied. The tissue origin of these tumors were discussed. Content Type Journal ArticleCategory Clinical ObservationsDOI 10.1007/BF03014411Authors Zhizhi Huang, Medical College of Shantou Un...

[Institutional report - Thoracic general] Sixty tracheal resections - single center experience

Interactive CardioVascular and Thoracic Surgery — Fri, 02 Jan 2009 05:00:00 +0100

This study evaluates the clinical outcome following surgery of our patients for the last seven years. Between 2001 and 2008 we performed tracheal resections in 60 patients. There were 46 cases of postintubation stenosis and 14 tumors. The range of resected rings was 1–8. The maximal resection length performed in our series (4 cm) was achieved using only basic releasing maneuvers such as anterior dissection of the trachea and cervical flexion. Emergency tracheal resection with no complications was performed in 12 patients who presented with severe dyspnea due to very tight stenosis. One patient died during the surgical intervention from a stroke. There were two postoperative deaths, both in patients with tracheo-esophageal fistula. As major complications we mention one patient wi...

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Effects of Panduratin A Isolated from Kaempferia pandurata ROXB. on the Expression of Matrix Metalloproteinase-9 by Porphyromonas gingivalis Supernatant-Induced KB Cells.

Biological and Pharmaceutical Bulletin — Thu, 01 Jan 2009 05:00:00 +0100

In this study, panduratin A, a natural bioactive compound isolated from Kaempferia pandurata ROXB., was used to test its in vitro inhibitory activity against MMP-9 secretion from Porphyromonas gingivalis supernatant-induced human oral epidermoid carcinoma KB cells. Gelatin zymography, Western blot and RT-PCR analyses were performed to evaluate MMP-9 expression. The gelatin zymograms revealed that the main gelatinase secreted by P. gingivalis supernatant-induced KB cells migrated at 92 kDa, representing MMP-9. MMP-9 protein and mRNA levels were significantly decreased after panduratin A treatment (p<0.05). In contrast, panduratin A had no effect on tissue inhibitor of metalloproteinases (TIMP)-1 and TIMP-2 mRNA. Panduratin A also suppressed urokinase type plasminogen activator (uPA) mRNA...

Metastatic Liposarcoma to the Parotid

Journal of Oncology — Mon, 15 Dec 2008 12:51:54 +0100

We report an exceptional case of parotid metastasis of myxoid liposarcoma in a 42-year-old man and insist on the worse prognosis of this entity. (Source: Journal of Oncology)

Discovering novel 3-nitroquinolines as a new class of anticancer agents1

Acta Pharmacologica Sinica — Sun, 14 Dec 2008 05:00:00 +0100

Conclusion: This study was the first to identify new structural types of antiproliferative agents against the EGFR-overexpressing tumor cell lines by the incorporation of the nitro group at the 3-position of the quinoline core structure, providing promising new templates for the further development of anticancer agents. (Source: Acta Pharmacologica Sinica)

Photodynamie activity on human larynx epidermoid carcinoma cell line, HEp-2

Medical Oncology — Fri, 05 Dec 2008 09:17:54 +0100

Content Type Journal ArticleCategory Research ReportsDOI 10.1007/BF02999864Authors S. Ganesan, Anna University Department of Physics 600 025 Madras IndiaV. Masitamani, Anna University Department of Physics 600 025 Madras India Journal Medical OncologyOnline ISSN 1559-131XPrint ISSN 1357-0560 Journal Volume Volume 11 Journal Issue Volume 11, Numbers 3-4 / September, 1994 (Source: Medical Oncology)

Radiation-induced carcinoma of the rectum

Diseases of the Colon & Rectum — Fri, 28 Nov 2008 14:08:53 +0100

We present a case of carcinoma of the rectum arising as two separate primaries 46 years after irradiation for an epidermoid carcinoma of the cervix. There were many pathologic changes related to the radiation therapy. Content Type Journal ArticleDOI 10.1007/BF02989000Authors Antonio Martins, Memorial Sloan-Kettering Cancer Center 1275 York Avenue 10021 New York New YorkStephen S. Sternberg, Memorial Sloan-Kettering Cancer Center 1275 York Avenue 10021 New York New YorkFadi F. Attiyeh, Memorial Sloan-Kettering Cancer Center 1275 York Avenue 10021 New York New York Journal Diseases of the Colon & RectumOnline ISSN 1530-0358Print ISSN 0012-3706 Journal Volume Volume 23 Journal Issue Volume 23, Number 8 / November, 1980 (Source: Diseases of the Colon & Rectum)

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Discovering novel 3-nitroquinolines as a new class of anticancer agents.

Acta Pharmacologica Sinica — Thu, 27 Nov 2008 07:34:55 +0100

Enediyne lidamycin enhances the effect of epidermal growth factor receptor tyrosine kinase inhibitor, gefitinib, in epidermoid carcinoma A431 cells and lung carcinoma H460 cells.

Anti-Cancer Drugs — Fri, 21 Nov 2008 08:13:58 +0100

Page: 41DOI: 10.1097/CAD.0b013e328318292cAuthors: Liu, Hong; Li, Liang; Li, Xing-Qi; Liu, Xiu-Jun; Zhen, Yong-Su (Source: Anti-Cancer Drugs)

[Tumors of the oral cavity: the usefulness of dynamic CT.]

Radiologia — Sat, 01 Nov 2008 04:00:00 +0100

CONCLUSIONS: The habitual contact between the mucosal surfaces of the oral cavity makes some clinically obvious lesions difficult to identify at CT. The interposition of air acts as a natural contrast, separating the gums from the mucosa of the buccomaseteric region; PCCT provides relevant information and should be included in the imaging workup in patients with tumors of the oral cavity. PMID: 19100214 [PubMed - in process] (Source: Radiologia)

[Tumors of the oral cavity: the usefulness of dynamic CT]

Radiologia — Sat, 01 Nov 2008 04:00:00 +0100

Metachronous bilateral mucoepidermoid carcinoma of the parotid gland

Indian Journal of Otolaryngology and Head & Neck Surgery — Wed, 22 Oct 2008 06:34:00 +0100

We report a very rare case of bilateral muco-epidermoid carcinoma of the parotid gland that underwent bilateral parotidectomy with neck dissections and radiotherapy. This case has done well for three years and suggests that metachronous bilateral mucoepidermoid carcinoma of the parotid gland, if treated as per the merits of each side, has a reasonable survival. Content Type Journal ArticleCategory Case ReportDOI 10.1007/s12070-008-0086-xAuthors John C. Muthusami, Christian Medical College Hospital Vellore 632 004 Tamil Nadu IndiaPranay Gaikwad, Christian Medical College Hospital Vellore 632 004 Tamil Nadu IndiaJohn P. Raj, Christian Medical College Hospital Vellore 632 004 Tamil Nadu IndiaDeepak T. Abraham, Christian Medical College Hospital Vellore 632 004 Tamil Nadu IndiaMeera Tho...

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G-protein-coupled receptor-associated A-kinase anchoring proteins AKAP5 and AKAP12: Differential trafficking and distribution.

Cellular Signalling — Fri, 10 Oct 2008 04:00:00 +0100

Authors: Chen MH, Malbon CC A-kinase Anchoring Proteins (AKAPs) define an expanding group of scaffold proteins that display a signature binding site for the RI/RII subunit of protein kinase A. AKAP5 and AKAP12 are multivalent (with respect to protein kinases and phosphatases) and display the ability to associate with the prototypic member of G protein-coupled receptors, the beta(2)-adrenergic receptor. We probed the relative abundance, subcellular distribution and localization of AKAP5 and AKAP12 in human embryonic kidney HEK293 and epidermoid carcinoma A431 cells. HEK293 cells are relatively rich in AKAP5 (found mostly in association with the cell membrane); whereas A431 cells are rich in AKAP12 (found distributed both in the cytoplasm and in association with the cell membrane). In bi...

[Scintigraphy in staging upper aerodigestive tract epidermoid carcinomas.]

Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale — Thu, 11 Sep 2008 04:00:00 +0100

CONCLUSION: The incidence of bone metastases in the initial extension assessment was low; consequently, they are not sought systematically. However, their presence radically changes the prognosis and the therapeutic management, raising the question of screening. The technetium-99m bone scintigraphy has limits, with many false-positive resulting the need for additional investigations. Defining the risk factors for bone metastases would improve screening. Two questions remain: what factors are involved? The bone pain and the hypercalcemia must be analyzed with a larger number of cases, but they seem to be nonspecific. The node involvement stage could be a more reliable parameter, in particular in cases of jugular vein invasion; what method should be used? In the future, the PET scan could be...

[Double simultaneous fingertip metastasis of an epidermoid carcinoma. A case report.]

Chirurgie de la Main — Thu, 04 Sep 2008 04:00:00 +0100

Authors: Ottomani S, Levadoux M, Cathelineau O, Nguyen M, Monchal T Fingertip metastases are rare. The authors report the case of a patient with an epidermoid carcinoma of the larynx who presented with two separate finger pulp metastases in each hand. According to the literature this seems a very unusual occurrence. This diagnosis must be considered in the differential diagnosis of every suspicious fingertip lesion. PMID: 18818116 [PubMed - as supplied by publisher] (Source: Chirurgie de la Main)

Reversal effect of resveratrol on multidrug resistance in KBv200 cell line.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie — Thu, 28 Aug 2008 04:00:00 +0100

Authors: Quan F, Pan C, Ma Q, Zhang S, Yan L A multidrug-resistant clone of human oral epidermoid carcinoma KB cells was isolated by stepwise selection on exposure to increasing doses of vincristine. The final clone, KBv200, obtained after ethylmethane sulfonate mutagenesis showed 156-fold higher resistance to vincristine than KB cells. The cells were also cross-resistant to paclitaxel and adriamycin. The aim of this study was to explore the reversal effect and potential mechanism of resveratrol on KBv200 cells. MTT assay was used to investigate the reversal index of resveratrol to vincristine, adriamycin and paclitaxel. Cell apoptosis was measured by flow cytometry. RT-PCR and western blot were used to detect mRNA and protein expression of multidrug-resistant gene MDR1 and apoptosis-s...

[Is it Necessary to Perform Neck Dissections as a Staged Procedure in Laser Supraglottic Laryngectomy?]

Acta Otorrinolaringologica Espanola — Fri, 01 Aug 2008 04:00:00 +0100

CONCLUSIONS: It is a safe procedure to perform neck dissections at the same time as the supraglottic laryngectomy. We did not find any increase in the complications rate and the hospital stay was shorter. PMID: 18817717 [PubMed - in process] (Source: Acta Otorrinolaringologica Espanola)

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Synthesis and in vitro antitumor activities of novel 4-anilinoquinazoline derivatives.

European Journal of Medicinal Chemistry — Sat, 26 Jul 2008 04:00:00 +0100

Authors: Chandregowda V, Kush AK, Chandrasekara Reddy G A series of 6, 7-dialkoxy-4-anilinoquinazolines were designed, synthesized by substituting different heterocycles on 6-position and a variety of anilines on 4-position of the quinazoline. These novel quinazoline compounds were screened for their cytotoxic effect on epidermal growth factor receptor overexpressing skin epidermoid carcinoma cell line (A431), by using nonoverexpressing tumor cells as negative control (breast adeno carcinoma cell line MCF-7). 2-Butyl-4-chloro-1-{3-[7-methoxy-4-(3-(trifluoromethyl)phenylamino)quinazolin-6-yloxy]-propyl}-1H-imidazole-5-carboxaldehyde (30) and 2-butyl-4-chloro-1-{3-[4-(3-iodophenyl amino)-7-methoxyquinazolin-6-yloxy]propyl}-1H-imidazole-5-carboxaldehyde (33) were found to be more potent a...

Epidermoid carcinoma involving the parotid gland and orbital region: Case report of a rare entity.

Ear, Nose and Throat Journal — Tue, 01 Jul 2008 04:00:00 +0100

Authors: Aslan S, Yavuz H, Altan-Yaycioglu R, Bolat F, Ozgirgin N Epidermoid carcinoma (also known as squamous cell carcinoma) of the parotid gland is more prevalent among the elderly, as are other head and neck tumors. In most cases it is observed either as a nodal metastasis of a primary tumor that drains into the parotid area or as a direct extension of a tumor that has originated in the upper dermis. Primary epidermoid carcinomas arising directly from the parotid gland are extremely rare. Similarly, secondary invasion of the orbital region by a neighboring tumor is more common than primary epidermoid carcinoma of the orbit, which is also rare. In this article, we discuss the diagnosis and treatment of a rare case of epidermoid carcinoma simultaneously invading the parotid gland and...

BCH, an Inhibitor of System L Amino Acid Transporters, Induces Apoptosis in Cancer Cells.

Biological and Pharmaceutical Bulletin — Wed, 04 Jun 2008 14:50:53 +0100

Conclusion: These results suggest that the inhibition of LAT1 activity by BCH leads to apoptotic cancer cell death by inducing intracellular depletion of neutral amino acids necessary for cancer cell growth. PMID: 18520037 [PubMed - as supplied by publisher] (Source: Biological and Pharmaceutical Bulletin)

Two new neo-clerodane diterpenoids from Scutellaria barbata.

Journal of Integrative Plant Biology — Sun, 01 Jun 2008 04:00:00 +0100

Authors: Dai SJ, Shen L, Ren Y Two new neo-clerodane diterpenoids, 6,7-dibenzoyloxybarbatin C (1, named barbatin D) and 6-(2-acetoxy-3-methylbutanoloxy)-7-(2-carbonyl-3-methylbutanoyloxy) barbatin C (2, named barbatin E) were isolated from the whole plant of Scutellaria barbata D. Don. Their structures were elucidated by spectroscopic methods including extensive 1D and 2D NMR analyses. In vitro, compounds 1-2 showed cytotoxic activities against three human cancer lines, namely, HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells, and with IC(50) values in the range of 3.5-6.7 microM. PMID: 18713410 [PubMed - in process] (Source: Journal of Integrative Plant Biology)

Incidental finding of an intrauterine device in the sigmoid colon.

The European Journal of Contraception and Reproductive Health Care — Sun, 01 Jun 2008 04:00:00 +0100

CONCLUSION: Asymptomatic migration of IUD to the sigmoid colon lumen can occur. Except when the patient's condition does not allow it, as in the case reported, removal of the IUD is indicated because of the risk of fistula formation and colon perforation with a high ensuing morbidity. This case report highlights the need for follow-up of patients wearing an IUD. PMID: 18465486 [PubMed - indexed for MEDLINE] (Source: The European Journal of Contraception and Reproductive Health Care)

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Cytotoxic activities of trichothecenes isolated from an endophytic fungus belonging to order hypocreales.

Archives of Pharmacal Research — Thu, 01 May 2008 04:00:00 +0100

Authors: Chinworrungsee M, Wiyakrutta S, Sriubolmas N, Chuailua P, Suksamrarn A Bioassay-guided fractionation of the extract of the endophytic fungus KLAR 5 belonging to order Hypocreales, which was isolated from the twig of Knema laurina (Blume) Warb., resulted in the isolation of brefeldin A (1), 8-deoxy-trichothecin (2), trichothecolone (3), 7alpha-hydroxytrichodermol (4), and 7alpha-hydroxyscirpene (5). Compound 5 was isolated from natural source for the first time. Compound 1 was very highly active against human epidermoid carcinoma of the mouth, human breast cancer (BC-1), and human small cell lung cancer (NCI-H187) cells whereas compounds 2 and 4 were selectively active against BC-1 and NCI-H187 cells. Compounds 3 and 5 were moderately active against these three cancer cell line...

[Partial pharyngolaryngectomies for treatment of pharyngolaryngeal cancer.]

Cirugia y Cirujanos — Thu, 01 May 2008 04:00:00 +0100

Conclusions: Voice conservation surgery is an alternative to mutilation of the larynx in patients with locally advanced neoplasms of the larynx with a high risk of recurrence if treated with radiotherapy. PMID: 18647554 [PubMed - in process] (Source: Cirugia y Cirujanos)

[Occupational exposure to wood dust and nasal sinus cancer.]

Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale — Tue, 22 Apr 2008 04:00:00 +0100

CONCLUSION: Epidemiologic data, such as the increasing incidence, and clinical and professional data, such as the occupational exposure to wood dust, were in agreement with the French and European literature. It is still probably too early to appreciate the effectiveness of prevention, established in France since 1980, on nasal sinus tumor incidence. PMID: 18436189 [PubMed - as supplied by publisher] (Source: Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale)

Soluble EMMPRIN (extra-cellular matrix metalloproteinase inducer) stimulates the migration of HEp-2 human laryngeal carcinoma cells, accompanied by increased MMP-2 production in fibroblasts.

Arch Histol Cytol — Tue, 01 Apr 2008 04:00:00 +0100

Authors: Hanata K, Yamaguchi N, Yoshikawa K, Mezaki Y, Miura M, Suzuki S, Senoo H, Ishikawa K The basement membrane functions as a barrier against the invasion of cancer cells. It is therefore important to investigate the mechanism of basement membrane degradation by matrix metalloproteinases (MMPs). Previously, cancer cells were long considered to be the major source of MMPs; however, current evidence indicates that most MMPs in cancer tissue are produced by stromal rather than cancer cells. A glycoprotein highly expressed on the cancer-cell membrane, EMMPRIN (extra-cellular matrix metalloproteinase inducer), exhibits the potential role of the MMP inductor in stromal cells. Depending on the cell type, EMMPRIN can stimulate the production of MMP-1, MMP-2, and MMP-3. We here report that...

[Breast metastasis from vulvar carcinoma: Case report and review of literature.]

Cancer radiotherapie — Fri, 14 Mar 2008 04:00:00 +0100

We report the case of a 42 year-old patient who underwent a treatment because of vulvar epidermoid carcinoma, right away metastatic at the level of the inguinal ganglia. The treatment consisted in a total vulvectomy with bilateral ganglial curretage, followed by external radiotherapy about the perineum and the inguinal ganglia. Three months after the end of her treatment, the patient presented with a nodula on the left outer breast with features of malignancy noticed by clinic and mammographic examination. The histologic study of the mammary biopsy showed epidermoid carcinoma of likely metastatic origin. A left Patey has been realized and confirmed the metastatic localization of epidermoid carcinoma with axillary ganglial metastasis (2N+/-7). Besides, this patient presented a right cervica...

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[Mouth floor and mobile tongue epidermoid carcinomas thickness: Prognostic value.]

Revue de Stomatologie et de Chirurgie Maxillo-Faciale — Wed, 05 Mar 2008 05:00:00 +0100

This study suggests that tumor thickness should be taken in consideration in T1N0, T2N0 and T3N0 mouth floor and mobile tongue epidermoid carcinomas. In the future, the clinical evaluation of tumor thickness will help determine the therapeutic management. PMID: 18328517 [PubMed - as supplied by publisher] (Source: Revue de Stomatologie et de Chirurgie Maxillo-Faciale)

Esophagus - Esophageal Squamous Papilloma

The Digital Atlas of Video Education - Gastroenterology — Sun, 02 Mar 2008 08:03:19 +0100

In conclusion squamous cell papilloma is an uncommon benign esophageal tumor which must be removed in all patients because of concern regarding malignant potential. (Source: The Digital Atlas of Video Education - Gastroenterology)

[A novel retinoid CD437 induces apoptosis of human epidermoid carcinoma A431 cells.]

Journal of Southern Medical University — Sat, 01 Mar 2008 05:00:00 +0100

CONCLUSION: CD437 is more effective than ATRA in inhibiting the proliferation and inducing apoptosis in A431 cells and shows selective apoptosis-inducing effect against malignant keratinocytes, suggesting its potential in the prevention or treatment of cutaneous carcinoma. PMID: 18359678 [PubMed - in process] (Source: Journal of Southern Medical University)

Safety of repeated administrations of ixabepilone given as a 3-hour infusion every other week in combination with irinotecan in patients with advanced malignancies.

European Journal of Cancer — Tue, 26 Feb 2008 05:00:00 +0100

In conclusion, toxicity including peripheral neuropathy was manageable at the recommended doses of 20 mg/m(2) ixabepilone combined with 180 mg/m(2) irinotecan on days 1 and 14 every 28 days. Promising antitumour activity was observed in patients with platinum-pretreated lung cancer. PMID: 18308561 [PubMed - as supplied by publisher] (Source: European Journal of Cancer)

Reconstruction of the Lower Lip: Bernard Technique and Its Variants

Dermatologic Surgery — Wed, 06 Feb 2008 15:56:27 +0100

Dermatologic Surgery, Volume 0, Issue 0, Page ???, OnlineEarly Articles. INTRODUCTION Surgical treatment of epidermoid carcinoma of the lower lip occasionally involves resection of a large part of this anatomic area. Several techniques are available for its repair, including Bernard's technique. Since its first description ... (Source: Dermatologic Surgery)

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Molecular mechanism of black tea polyphenols induced apoptosis in human skin cancer cells: involvement of Bax translocation and mitochondria mediated death cascade

Carcinogenesis — Thu, 31 Jan 2008 05:00:00 +0100

This study investigated the induction of apoptosis in human skin cancer cells after treatment of TF and TR. We report that both TF and TR could exert inhibition of A431 (human epidermoid carcinoma) and A375 (human malignant melanoma) cell proliferation without adversely affecting normal human epidermal keratinocyte cells. Growth inhibition of A375 cells occurred through apoptosis, as evident from cell cycle arrest at G0/G1 phase, increase in early apoptotic cells, externalization of phosphatidylserine and DNA fragmentation. In our pursuit to dissect the molecular mechanism of TF- and TR-induced apoptosis in A375 cells, we investigated whether cell death is being mediated by mitochondria. In our system, Bax translocation to mitochondria persuaded depolarization of mitochondrial membrane pot...

Lymphatic mapping and sentinel lymph node biopsy in epidermoid carcinoma of the anal canal.

European Journal of Surgical Oncology — Fri, 04 Jan 2008 05:00:00 +0100

CONCLUSIONS: Inguinal sentinel node biopsy in anal cancer is efficient and could assist in the decision for inguinal radiation. The validity and safety of the proposed therapeutic algorithm has to be proven by a larger, prospective study. PMID: 18178364 [PubMed - as supplied by publisher] (Source: European Journal of Surgical Oncology)

Diverse TNFalpha-induced death pathways are enhanced by inhibition of NF-kappaB.

International Journal of Oncology — Sat, 01 Dec 2007 05:00:00 +0100

Authors: Katdare M, Efimova EV, Labay E, Khodarev NN, Darga TE, Garofalo M, Nakamura S, Kufe DW, Posner MC, Weichselbaum RR TNFalpha was initially described as inducing necrotic death in tumors in vivo, and more recently as a cytokine that mediates cytoprotection and inflammation. The anti-tumor effects of TNFalpha are poorly characterized because TNFalpha-induced death of human tumor cells has largely been studied in the presence of agents that block transcription or protein synthesis. Also, most reports in model cell systems describe apoptosis within relatively early time points as the principal mode of cell death induced by TNFalpha. We investigated the cytotoxic effects of 10 ng/ml TNFalpha on human tumor cells of different histological types without concomitant exposure to these i...

[Systematic neck dissection in squamous cell carcinoma of the oral cavity]

Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale — Sat, 01 Dec 2007 05:00:00 +0100

CONCLUSION: The systematic cervical lymph node dissection remains the most effective means to obtain the neck control of squamous cell cancers of the oral cavity. It however was applied without therapeutic value for 45% of the patients of this series. The validation of the sentinel node concept as a method of cervical staging should make it possible to avoid this surgical procedure in more than one third of the cases. PMID: 17673159 [PubMed - indexed for MEDLINE] (Source: Annales d'Oto-laryngologie et de Chirurgie Cervico Faciale)