MedWorm: Parvovirus

SeraCare Launches Five New Infectious Disease Panels Including First...

PRWeb: Medical Pharmaceuticals — Fri, 03 Feb 2012 06:24:02 +0100

SeraCare Life Sciences announced it has launched new infectious disease panels for HIV, HBV, HAV and CMV markers, and a first-of-its-kind performance panel for parvovirus B19, most commonly associated...(PRWeb January 30, 2012)Read the full story at http://www.prweb.com/releases/2012/1/prweb9142297.htm (Source: PRWeb: Medical Pharmaceuticals)

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Genotyping of Human parvovirus B19 among Brazilian patients with hemoglobinopathies

Canadian Journal of Microbiology — Sat, 28 Jan 2012 08:13:49 +0100

Canadian Journal of Microbiology, Volume 0, Issue 0, Page 200-205, e-First articles. (Source: Canadian Journal of Microbiology)

Coping with parvovirus infections in mice: health surveillance and control

Laboratory Animals — Wed, 25 Jan 2012 05:00:00 +0100

Parvoviruses of mice, minute virus of mice (MVM) and mouse parvovirus (MPV), are challenging pathogens to eradicate from laboratory animal facilities. Due to the impediment on rodent-based research, recent studies have focused on the assessment of re-derivation techniques and parvoviral potential to induce persistent infections. Summarizing recent data, this review gives an overview on studies associated with parvoviral impact on research, diagnostic methods, parvoviral persistence and re-derivation techniques, demonstrating the complex nature of parvovirus infection in mice and unfolding the challenge of controlling parvovirus infections in laboratory animal facilities. (Source: Laboratory Animals)

Potential Infectious Etiology of Behçet's Disease.

Herpes — Fri, 20 Jan 2012 03:30:02 +0100

Authors: Galeone M, Colucci R, D'Erme AM, Moretti S, Lotti T Abstract Behçet's disease is a multisystem inflammatory disorder characterized by recurrent oral aphthous ulcers, genital ulcers, uveitis, and skin lesions. The cause of Behçet's disease remains unknown, but epidemiologic findings suggest that an autoimmune process is triggered by an environmental agent in a genetically predisposed individual. An infectious agent could operate through molecular mimicry, and subsequently the disease could be perpetuated by an abnormal immune response to an autoantigen in the absence of ongoing infection. Potentia bacterial are Saccharomyces cerevisiae, mycobacteria, Borrelia burgdorferi, Helicobacter pylori, Escherichia coli, Staphylococcus aureus, and Mycoplasma fermentans, but the most...

Acute generalized exanthematous pustulosis to amoxicillin associated with parvovirus B19 reactivation.

Cutaneous and Ocular Toxicology — Wed, 18 Jan 2012 05:00:00 +0100

We report the case of a 22-year-old male patient with 2 episodes, 4 months apart, of acute generalized exanthematous pustulosis (AGEP) associated with oral intake of amoxicillin and simultaneous reactivation of parvovirus B19 infection proven by positive polymerase chain reaction test in the skin fragment and blood sample and elevation of the IgG antibodies titer. To our knowledge, this is the first report of AGEP resulting from the interaction between drug hypersensitivity and the reactivation of parvovirus B19. A combination of an immunological reaction to the drug and virus infection could be responsible for the clinical picture. PMID: 22257115 [PubMed - as supplied by publisher] (Source: Cutaneous and Ocular Toxicology)

Emerging and Re‐emerging Swine Viruses

Transboundary and Emerging Diseases — Mon, 09 Jan 2012 05:00:00 +0100

This article focuses on emerging and re‐emerging swine viruses that have a limited or uncertain clinical and economic impact on pig health. The transmission, epidemiology and pathogenic potential of these viruses are discussed. In addition, the two economically important emerging viruses, PRRSV and PCV2, are also briefly discussed to identify important knowledge gaps. (Source: Transboundary and Emerging Diseases)

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Memory T cell inflation: understanding cause and effect.

Trends in Immunology — Tue, 03 Jan 2012 05:00:00 +0100

Authors: O'Hara GA, Welten SP, Klenerman P, Arens R Abstract Typically, during viral infections, T cells encounter antigen, undergo proliferative expansion and ultimately contract into a pool of memory cells. However, after infection with cytomegalovirus, a ubiquitous β-herpesvirus, T cell populations specific for certain epitopes do not contract but instead are maintained and/or accumulate at high frequencies with a characteristic effector-memory phenotype. This feature has also been noted after other infections, for example, by parvoviruses. We discuss this so-called memory T cell inflation and the factors involved in this phenomenon. Also, we consider the potential therapeutic use of memory T cell inflation as a vaccine strategy and the associated implications for immune senesc...

Focal segmental glomerulosclerosis and parvovirus B19.

Baylor University Medical Center Proceedings — Sun, 01 Jan 2012 05:00:00 +0100

Authors: Sanchez C, Fenves A, Schwartz J PMID: 22275777 [PubMed - in process] (Source: Baylor University Medical Center Proceedings)

Rabbit Antithymocyte Globulin Compared with Basiliximab in Kidney Transplantation: A Single-Center Study

Transplantation Proceedings — Sun, 01 Jan 2012 05:00:00 +0100

Conclusions: ATG is safe and efficacious for use in kidney transplant recipients. Our results suggest that ATG should be considered for induction therapy in high-risk patients, such as those who have a kidney allograft from a deceased donor, high levels of panel reactive antibodies, and are undergoing retransplantation. (Source: Transplantation Proceedings)

Characterization of the partial VP2 gene region of canine parvoviruses in domestic cats from Turkey

Virus Genes — Fri, 30 Dec 2011 06:48:23 +0100

In this study, molecular detection and genetic analysis of a partial VP2 gene region of CPVs from domestic cats living in Turkey between 2006 and 2010 was performed by PCR amplification and sequence analysis. The results indicated that CPV-2a, CPV-2c, and FPLV were circulating in vaccinated and unvaccinated cats. This is the first description of molecular characterization of CPVs in domestic cats from Turkey. Content Type Journal ArticlePages 1-8DOI 10.1007/s11262-011-0703-8Authors Dilek Muz, Department of Virology, Faculty of Veterinary Medicine, Mustafa Kemal University, Antakya, Hatay, TurkeyT. Çiğdem Oğuzoğlu, Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, TurkeyM. Özkan Timurkan, Department of Virology, Faculty of Veterinary Medicine,...

An oral Sindbis virus replicon-based DNA vaccine containing VP2 gene of canine parvovirus delivered by Escherichia coli elicits immune responses in dogs.

Acta Virologica — Sun, 25 Dec 2011 13:48:04 +0100

This study demonstrated that the oral CPV DNA vaccine delivered by E. coli can be considered as a promising approach for vaccination of dogs against CPV. Keywords: canine parvovirus; DNA vaccine; VP2; Sindbis virus replicon; E. coli. PMID: 22149493 [PubMed - in process] (Source: Acta Virologica)

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Detection of human bocavirus from children and adults with acute respiratory tract illness in Guangzhou, southern China

BMC Infectious Diseases — Wed, 14 Dec 2011 05:00:00 +0100

Background: Human bocavirus (HBoV) is a newly discovered parvovirus associated with acute respiratory tract illness (ARTI) and gastrointestinal illness. Our study is the first to analyze the characteristics of HBoV-positive samples from ARTI patients with a wide age distribution from Guangzhou, southern China. Methods: Throat swabs (n = 2811) were collected and analyzed from children and adults with ARTI over a 13-month period. The HBoV complete genome from a 60 year-old female patient isolate was also determined. Results: HBoV DNA was detected in 65/2811 (2.3%) samples, of which 61/1797 were from children ( (Source: BMC Infectious Diseases)

Wells’ Syndrome Associated with Parvovirus in a 5‐Year Old Boy

Pediatric Dermatology — Fri, 09 Dec 2011 05:00:00 +0100

Abstract: Wells’ syndrome, otherwise known as eosinophilic cellulitis, is a rare dermatosis seen more commonly in adults than in children. In this article, we present a 5‐year‐old Caucasian boy who initially presented with pruritic, erythematous macules and papules evolving to bullae formation. Subsequent histology confirmed diagnosis of Wells’ syndrome, and additional blood work also demonstrated positive immunoglobulin (Ig)M and IgG for parvovirus. In many instances, the direct etiology of Wells’ syndrome is unclear, but the link between parvoviral infection and development of Wells’ syndrome could further support an additional cause. (Source: Pediatric Dermatology)

Fetal Anemia of Unknown Cause - A Diagnostic Challenge.

Ultraschall in der Medizin — Fri, 09 Dec 2011 05:00:00 +0100

CONCLUSION: In cases of fetal anemia with negative indirect Coombs test and TORCH serology, rare causes of anemia have to be considered. Fetal studies should therefore include reticulocyte count, parameters of hemolysis, peripheral blood smear and fetal liver function tests. Maternal studies should involve a search for fetal red cells using flow cytometry rather than Kleihauer-Betke test. PMID: 22161617 [PubMed - as supplied by publisher] (Source: Ultraschall in der Medizin)

Development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus.

Journal of Virological Methods — Tue, 06 Dec 2011 05:00:00 +0100

In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for rapid, specific and sensitive detection of Pbo-likeV. A set of four primers specific for six regions of Pbo-likeV VP1/2 genes was designed with the online software. The reaction temperature and time were optimized to 65°C and 60min, respectively. LAMP products were detected by agarose gel electrophoresis or by visual inspection of a color change due to addition of fluorescent dye. The developed method was highly specific for detection of Pbo-likeV, and no cross-reaction was observed with other swine viruses, such as porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), porcine parvovirus (PPV) and classic swine fever virus (CSFV) found commonly in China. The lowe...

Clinical Inquiry: What should you tell pregnant women about exposure to parvovirus?

The Journal of Family Practice — Thu, 01 Dec 2011 05:00:00 +0100

Authors: Snyder M, Wallace R Abstract Tell patients that parvovirus infections before 20 weeks' gestation confer a risk of fetal morbidity and mortality as high as 16%, but don't significantly increase long-term developmental sequelae. PMID: 22163362 [PubMed - in process] (Source: The Journal of Family Practice)

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When are amniotic fluid viral PCR studies indicated in prenatal diagnosis?

Prenatal Diagnosis — Thu, 01 Dec 2011 05:00:00 +0100

ConclusionAmniotic fluid viral PCR testing should be considered for fetuses with intrauterine growth restriction, nonimmune hydrops fetalis, hand/foot anomalies, or NTDs. After aneuploidy is excluded, NTDs are associated with PCR positivity. © 2012 John Wiley & Sons, Ltd. (Source: Prenatal Diagnosis)

Mycoplasma pneumoniae Infection Presenting as Bullous Papular Purpuric Gloves and Socks Syndrome: Novel Association and Review of the Literature

Clinical Pediatrics — Fri, 25 Nov 2011 05:00:00 +0100

This study describes a case resulting from Mycoplasma pneumoniae infection in an adolescent male and reviews the literature. (Source: Clinical Pediatrics)

Diagnosis, management and possibilities to prevent parvovirus B19 infection in pregnancy

Future Virology — Thu, 24 Nov 2011 16:35:25 +0100

Future Virology , December 2011, Vol. 6, No. 12, Pages 1435-1450. (Source: Future Virology)

High frequency of Human Bocavirus 1 DNA in infants and adults with lower acute respiratory infection.

Journal of Medical Microbiology — Thu, 24 Nov 2011 05:00:00 +0100

Authors: Ghietto LM, Camara A, Camara J, Adamo MP Abstract Human Bocavirus (HBoV) is a parvovirus with a possible etiological role in respiratory disease currently under investigation. We detected HBoV1 in children and adults hospitalized with acute disease of the lower respiratory tract. HBoV genome was detected by PCR in nasopharyngeal swabs collected from 75 patients aged 0-89 years old during 2010. HBoV was found in 17/75 (22.7%) patients, 64.7% of them infants younger than 1 year old and 29.4% adults older than 30 years (the bimodal age distribution among HBoV positive patients was a statistically significant, p<0.001). Of all HBoV+ cases, 35.3% were co-infected; all co-infections occurred in children (≤ 13 years old) and 83.3% of them were HBoV-Respiratory Syncytial Viru...

A multiple SYBR Green I-based real-time PCR system for the simultaneous detection of porcine circovirus type 2, porcine parvovirus, pseudorabies virus and Torque teno sus virus 1 and 2 in pigs.

Journal of Virological Methods — Fri, 18 Nov 2011 05:00:00 +0100

Authors: Pérez LJ, Perera CL, Frías MT, Núñez JI, Ganges L, Arce HD Abstract Multiple viral infections are common in pigs under intensive production conditions. All five of the viruses included in this study are associated with multifactorial diseases that cause significant economic losses in swine farming worldwide. The development is described of a novel multiple real-time PCR system based on the use of SYBR Green I that allows the simultaneous detection and differentiation of porcine circovirus 2 (PCV-2), porcine parvovirus (PPV), pseudorabies virus (PRV) and Torque teno sus virus species 1 and 2 (TTSuV1 and TTSuV2) in pigs. The method was able to distinguish between all five viral agents, and tests of other DNA viruses proved the specificity of the system. The multiple real...

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Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs.

Journal of Virological Methods — Fri, 18 Nov 2011 05:00:00 +0100

Authors: Yi L, Cheng S, Xu H, Wang J, Cheng Y, Yang S, Luo B Abstract A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). A pair of primers (P1/P2) was used to detect both CDV wild-type strains and vaccines. Another pair (P3/P4) was used to detect only CDV wild-type strains. A 335bp fragment was amplified from the genomic RNA of the vaccine and wild-type strains. A 555bp fragment was amplified specifically from the genomic RNA of the wild-type strains. No amplification was achieved for the uninfected cells, cells infected with canine parvovirus, canine coronavirus, or canine adenovirus. The combined RT-PCR method detected effectively and d...

Evidence for evolutionary convergence at MHC in two broadly distributed mesocarnivores

Immunogenetics — Wed, 16 Nov 2011 06:50:25 +0100

Abstract Variation within major histocompatibility complex (MHC) genes is important in recognizing pathogens and initiating an immune response. These genes are relevant in enhancing our understanding of how species cope with rapid environmental changes and concomitant fluctuations in selective pressures such as invasive, infectious diseases. Disease-based models suggest that diversity at MHC is maintained through balancing selection arising from the coevolution of hosts and pathogens. Despite intensive balancing selection, sequence motifs or even identical MHC alleles can be shared across multiple species; three potential mechanisms have been put forth to explain this phenomenon: common ancestry, convergent evolution, and random chance. To understand the processes that mai...

Seroprevalence of porcine reproductive and respiratory syndrome, Aujeszky’s disease, and porcine parvovirus in replacement gilts in Thailand

Tropical Animal Health and Production — Wed, 09 Nov 2011 07:01:27 +0100

Abstract The present study investigated the seroprevalence of porcine reproductive and respiratory syndrome virus, Aujeszky’s disease virus (ADV), and porcine parvovirus (PPV) in replacement gilts from selected five swine herds in Thailand. The study consisted of three parts. First, a retrospective data analysis on the seroprevalence of porcine reproductive and respiratory syndrome virus (PRRSV) and ADV glycoprotein I (gI) in gilts, sows, boars, nursery, and fattening pigs in five herds (n = 7,030). Second, a cross-sectional study on seroprevalence of PRRSV, ADV, and PPV (n = 200) in replacement gilts. Last, the seroprevalence of PRRSV, ADV, and PPV in gilts culled due to reproductive failure (n = 166). Across the herds, the seroprevalence of PRRSV and ADV was ...

Seroprevalence of porcine reproductive and respiratory syndrome, Aujeszky's disease, and porcine parvovirus in replacement gilts in Thailand.

Adv Data — Wed, 09 Nov 2011 05:00:00 +0100

Authors: Tummaruk P, Tantilertcharoen R Abstract The present study investigated the seroprevalence of porcine reproductive and respiratory syndrome virus, Aujeszky's disease virus (ADV), and porcine parvovirus (PPV) in replacement gilts from selected five swine herds in Thailand. The study consisted of three parts. First, a retrospective data analysis on the seroprevalence of porcine reproductive and respiratory syndrome virus (PRRSV) and ADV glycoprotein I (gI) in gilts, sows, boars, nursery, and fattening pigs in five herds (n = 7,030). Second, a cross-sectional study on seroprevalence of PRRSV, ADV, and PPV (n = 200) in replacement gilts. Last, the seroprevalence of PRRSV, ADV, and PPV in gilts culled due to reproductive failure (n = 166). Across the herds, the serop...

Loop-mediated isothermal amplification for detection of porcine circovirus type 2

Virology Journal — Wed, 02 Nov 2011 04:00:00 +0100

Conclusions: LAMP is an useful rapid detection method with high sensitivity and specificity for PCV2. (Source: Virology Journal)

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Virologic and clinical features of primary infection with human parvovirus 4 in subjects with hemophilia: frequent transmission by virally inactivated clotting factor concentrates.

Transfusion — Wed, 02 Nov 2011 04:00:00 +0100

CONCLUSION: This study identifies PARV4 as a transfusion-transmissible agent that is resistant to viral inactivation. Of concern, infections may still regularly occur in those exposed to plasma-derived blood products. Urgent evaluation of the incidence of PARV4 in treated individuals and disease associations of PARV4 infections is required. PMID: 22043925 [PubMed - as supplied by publisher] (Source: Transfusion)

Interferon γ improves the vaccination potential of oncolytic parvovirus H-1PV for the treatment of peritoneal carcinomatosis in pancreatic cancer.

Cancer Biology and Therapy — Tue, 01 Nov 2011 09:10:35 +0100

Authors: Grekova SP, Aprahamian M, Daeffler L, Leuchs B, Angelova A, Giese T, Galabov A, Heller A, Giese NA, Rommelaere J, Raykov Z Abstract Oncolytic viruses with their capacity to specifically replicate in and kill tumor cells emerged as a novel class of cancer therapeutics. Rat oncolytic parvovirus (H-1PV) was used to treat different types of cancer in preclinical settings and was lately successfully combined with standard gemcitabine chemotherapy in treating pancreatic ductal adenocarcinoma (PDAC) in rats. Our previous work showed that the immune system and particularly the release of interferon-gamma (IFNγ ) seem to mediate the anticancer effect of H-1PV in that model. Therefore, we reasoned that the therapeutic properties of H-1PV can be boosted with IFNγ for the treatment ...

Severe glomerulonephritis and encephalopathy associated with parvovirus B19 infection mimicking systemic lupus erythematosus.

Scandinavian Journal of Rheumatology — Tue, 01 Nov 2011 04:00:00 +0100

Authors: Cugler T, Carvalho LD, Facincani I, Yamamoto A, Silva GB, Costa RS, Ferriani V PMID: 22043904 [PubMed - as supplied by publisher] (Source: Scandinavian Journal of Rheumatology)

Is routine viral screening useful in patients with recent‐onset polyarthritis of a duration of at least 6 weeks? Results from a nationwide longitudinal prospective cohort study

Arthritis Care and Research — Tue, 01 Nov 2011 04:00:00 +0100

ConclusionRoutine serologic testing did not contribute substantially to the diagnosis in this context. (Source: Arthritis Care and Research)

Evaluation of the human host range of bovine and porcine viruses that may contaminate bovine serum and porcine trypsin used in the manufacture of biological products.

Tue, 01 Nov 2011 04:00:00 +0100

Authors: Marcus-Sekura C, Richardson JC, Harston RK, Sane N, Sheets RL Abstract Current U.S. requirements for testing cell substrates used in production of human biological products for contamination with bovine and porcine viruses are U.S. Department of Agriculture (USDA) 9CFR tests for bovine serum or porcine trypsin. 9CFR requires testing of bovine serum for seven specific viruses in six families (immunofluorescence) and at least 2 additional families non-specifically (cytopathicity and hemadsorption). 9CFR testing of porcine trypsin is for porcine parvovirus. Recent contaminations suggest these tests may not be sufficient. Assay sensitivity was not the issue for these contaminations that were caused by viruses/virus families not represented in the 9CFR screen. A detailed litera...

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A TaqMan Real-Time PCR Assay for Quantifying Type III Hepatopancreatic Parvovirus Infections in Wild Broodstocks and Hatchery-Reared Postlarvae of Fenneropenaeus chinensis in Korea.

Journal of Microbiology and Biotechnology — Tue, 01 Nov 2011 04:00:00 +0100

Authors: Jang IK, Suriakala K, Kim JS, Meng XH, Choi TJ Abstract A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatcheryreared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained from seven and six commercial hatcheries in 2007 and 2008, respectively. Among the three wild broodstock groups from 2007, only 1 group showed HPV infection and 3.2% of 159 brooders were positive for HPV infection. In 2008, HPV infections were observed from all three wild broodstock groups with 1.93×10(4) copies/mg tissue of pleopods. Of 162 brooders, 26.6% were positive for HPV infection. No PL from the t...

Activation of the human immune system by chemotherapeutic or targeted agents combined with the oncolytic parvovirus H-1

BMC Cancer — Wed, 26 Oct 2011 04:00:00 +0100

Conclusions: In our human models, chemotherapeutic or targeted agents did not only interfere with the pronounced immunomodulatory properties of H-1PV, but also reinforced drug-induced tumor cell killing. H-1PV combined with cisplatin, vincristine or sunitinib induced effective immunostimulation via a pronounced DC maturation, better cytokine release and cytotoxic T-cell activation compared with agents alone. Thus, the clinical assessment of H-1PV oncolytic tumor therapy not only alone but also in combination strategies is warranted. (Source: BMC Cancer)

Parvovirus B19 infection and amniocentesis

Prenatal Diagnosis — Mon, 24 Oct 2011 05:04:11 +0100

Parvovirus B19 infection in pregnancy and amniocentesis

Prenatal Diagnosis — Mon, 24 Oct 2011 05:04:10 +0100

(Source: Prenatal Diagnosis)

Positive Borrelia burgdorferi serology secondary to intravenous immunoglobulin therapy

Journal of Infection — Mon, 24 Oct 2011 04:00:00 +0100

We describe a case where misleading positive Borrelia burgdorferi antibodies were caused by the administration of intravenous immunoglobulin. (Source: Journal of Infection)

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Rare, unusual, and less common virus infections after organ transplantation.

Current Opinion in Organ Transplantation — Thu, 13 Oct 2011 04:00:00 +0100

Authors: Razonable RR Abstract PURPOSE OF REVIEW: The present article reviews the epidemiology, clinical presentation, prevention, and management of rare, unusual, and less common viruses that infect transplant recipients. RECENT FINDINGS: Infection may be acquired as a result of natural transmission, reactivation of latent virus, or transmission through the allograft or blood transfusion. The epidemiology, clinical manifestations, and management of these viruses vary widely. Some viruses such as human herpesviruses 6 and 7 are ubiquitous in humans, but they rarely cause clinical disease after organ transplantation. Likewise, adenoviruses, parvoviruses, and some polyomaviruses are commonly transmitted infections in the community, but they cause clinical syndromes rarely in transpla...

Structure-function analysis of receptor-binding in adeno-associated virus serotype 6 (AAV-6).

Virology — Sat, 08 Oct 2011 17:26:09 +0100

Authors: Xie Q, Lerch TF, Meyer NL, Chapman MS Abstract Crystal structures of the AAV-6 capsid at 3Å reveal a subunit fold homologous to other parvoviruses with greatest differences in two external loops. The electrostatic potential suggests that receptor-attachment is mediated by four residues: Arg(576), Lys(493), Lys(459) and Lys(531), defining a positively charged region curving up from the valley between adjacent spikes. It overlaps only partially with the receptor-binding site of AAV-2, and the residues endowing the electrostatic character are not homologous. Mutational substitution of each residue decreases heparin affinity, particularly Lys(531) and Lys(459). Neither is conserved among heparin-binding serotypes, indicating that diverse modes of receptor attachment have been...

The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus

Virology Journal — Fri, 07 Oct 2011 04:00:00 +0100

This report describes a one-step real-time polymerase chain reaction assay based on SYBR Green I for detection of a broad range of duck circovirus (DuCV). Align with all DuCV complete genome sequences and other Genus Circovirus download from the GenBank (such as goose circovirus, pigeon circovirus), the primers targets to the replicate gene of DuCV were designed. The detection assay was linear in the range of 1.31x102-1.31x107 copies/muL. The reaction efficiency of the assay using the slope (the slope was -3.349) and the Y-intercept was 37.01 from the linear equation was estimated to be 0.99 and the correlation coefficient (R2) was 0.993. A series of experiments were carried out to assess the reproducibility, sensitivity, and specificity of the assay, following by the low intra-assay and i...

Validation of model virus removal and inactivation capacity of an erythropoietin purification process.

Fri, 07 Oct 2011 04:00:00 +0100

In conclusion, this study corroborated that the assessed rec-hEpo purification process has enough capacity (5.0-19.4 Logs) for removing and inactivating these model viruses and sodium hydroxide demonstrated to be a robust sanitization solution for chromatography columns (5.0 (PV-2)-6.7 (CPV) Logs). PMID: 21982851 [PubMed - as supplied by publisher] (Source: Biologicals : Journal of the International Association of Biological Standardization)

Infectious microorganisms in mice (Mus musculus) purchased from commercial pet shops in Germany

Laboratory Animals — Mon, 03 Oct 2011 04:00:00 +0100

In this study, we investigated the prevalence of infectious microorganisms (viruses, bacteria, fungi and eukaryotic parasites) in mice from different pet shops in Germany; such animals may compromise the hygienic integrity of laboratory animal vivaria if private pet holders act as unintended vectors of infections carried by them. House mice sold as pets or feed specimens were purchased from different pet shops and tested for a comprehensive panel of unwanted microorganisms. We found a number of microorganisms in these pet shop mice, the most prevalent of which were Helicobacter species (92.9%), mouse parvovirus (89.3%), mouse hepatitis virus (82.7%), Pasteurella pneumotropica (71.4%) and Syphacia species (57.1%). Several microorganisms (e.g. mouse parvovirus, Theiler's murine encephalomyel...

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Acute fulminant hepatitis with bone marrow failure in an adult due to parvovirus B19 infection

Hepatology — Mon, 03 Oct 2011 04:00:00 +0100

(Source: Hepatology)

Distribution and genetic diversity of porcine hokovirus in wild boars

Archives of Virology — Sat, 01 Oct 2011 06:46:23 +0100

Abstract Porcine hokovirus (PHoV), a newly discovered member of the family Parvoviridae and the proposed genus Hokovirus, is considered phylogenetically distinct from other parvoviruses. Here, we report a comprehensive spatio-temporal study of PHoV infection in Romanian wild boars. The prevalence of PHoV differed significantly in samples from 2006/2007 (22.76%) and 2010/2011 (50.54%), and also increased with age. Sequence analysis of PHoVs from 2006/2007 showed a close relationship to PHoVs from pigs from England and wild boars from Germany, while the PHoVs from 2010/2011 were mostly similar to isolates from Hong Kong. The most variable regions were detected in the NS1 gene and proved to be suitable for analysis of the genetic diversity of the virus. It was observed that PH...

Collaborative study to establish a World Health Organization International genotype panel for parvovirus B19 DNA nucleic acid amplification technology (NAT)‐based assays

Vox Sanguinis — Sat, 01 Oct 2011 04:00:00 +0100

Conclusions The four‐member panel is intended for use in evaluating the ability of NAT assays to detect different B19V genotypes (M1–M3). Based on the results of the collaborative study, the panel was established as the 1st WHO International Reference Panel for parvovirus B19 genotypes. (Source: Vox Sanguinis)

Placental transmission of human parvovirus 4 in newborns with hydrops, taiwan.

Emerging Infectious Diseases — Sat, 01 Oct 2011 04:00:00 +0100

Authors: Chen MY, Yang SJ, Hung CC Abstract In studying the epidemiology of parvovirus 4 (PARV4) in Taiwan, we detected DNA in plasma of 3 mothers and their newborns with hydrops. In 1 additional case, only the mother had PARV4 DNA. Our findings demonstrate that PARV4 can be transmitted through the placenta. PMID: 22000381 [PubMed - in process] (Source: Emerging Infectious Diseases)

Novel amdovirus in gray foxes.

Emerging Infectious Diseases — Sat, 01 Oct 2011 04:00:00 +0100

Authors: Li L, Pesavento PA, Woods L, Clifford DL, Luff J, Wang C, Delwart E Abstract We used viral metagenomics to identify a novel parvovirus in tissues of a gray fox (Urocyon cinereoargenteus). Nearly full genome characterization and phylogenetic analyses showed this parvovirus (provisionally named gray fox amdovirus) to be distantly related to Aleutian mink disease virus, representing the second viral species in the Amdovirus genus. PMID: 22000359 [PubMed - in process] (Source: Emerging Infectious Diseases)

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Modigraf 0.2mg & 1mg granules for oral suspension (tacrolimus) - Revised SPC

NeLM - SPC Changes — Fri, 30 Sep 2011 04:00:00 +0100

Source: eMC (electronic Medicines Compendium) Area: Other Library Updates > SPC Changes The SPC has been updated to reflect the following changes: Section 4.4 Special warnings and precautions for use Addition of text: Pure Red Cell Aplasia Cases of pure red cell aplasia (PRCA) have been reported in patients treated with tacrolimus. All patients reported risk factors for PRCA such as parvovirus B19 infection, underlying disease or concomitant medications associated with PRCA. Section 4.8 Undesirable effects Blood and lymphatic system disorders : pure red cell aplasia has been added to the Undesirable effects, with the frequency as not known. Please refer to SPC link below for full information (Source: NeLM...

Advagraf 0.5mg, 1mg, 3mg and 5mg Prolonged-release hard capsules (tacrolimus)- Revised SPC

NeLM - SPC Changes — Fri, 30 Sep 2011 04:00:00 +0100

Source: eMC (electronic Medicines Compendium) Area: Other Library Updates > SPC Changes The SPC for Advagraf (tacrolimus) has been updated to reflect changes in the following sections: Section 4.4 Special warnings and precautions for use The following text has been added: Pure Red Cell Aplasia Cases of pure red cell aplasia (PRCA) have been reported in patients treated with tacrolimus. All patients reported risk factors for PRCA such as parvovirus B19 infection, underlying disease or concomitant medications associated with PRCA. Section 4.8 Undesirable effects Blood and lymphatic system disorders : pure red cell aplasia has been added to the Undesirable effects, with the frequency as not known. Please refer to the SPC link below for full information....

Is routine viral screening useful in patients with recent‐onset polyarthritis of at least 6 weeks' duration (ESPOIR Cohort Study)?

Arthritis Care and Research — Fri, 30 Sep 2011 00:23:04 +0100

[Childhood parvovirus B19 encephalitis.]

Archives de Pediatrie — Thu, 29 Sep 2011 04:00:00 +0100

CONCLUSION: Even if PVB19 research is not cited in the French or American infectious disease society recommendations on the diagnosis and management of infectious encephalitis, this virus may be responsible, especially in cases of child febrile rash. Therefore, PVB19 research seems reasonable if the clinical presentation is concordant in children due to its diagnostic simplicity and efficacy. PMID: 21963073 [PubMed - as supplied by publisher] (Source: Archives de Pediatrie)

Widespread Horizontal Gene Transfer from Circular Single-stranded DNA Viruses to Eukaryotic Genomes

BMC Evolutionary Biology - Latest articles — Mon, 26 Sep 2011 04:00:00 +0100

Conclusions: Our discovery extends the host range of circular ssDNA viruses and sheds light on the origin and evolution of these viruses. It also suggests that ssDNA viruses act as an unforeseen source of genetic innovation in their hosts. (Source: BMC Evolutionary Biology - Latest articles)

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Complete nucleotide sequence analysis of a Korean strain of hepatopancreatic parvovirus (HPV) from Fenneropenaeus chinensis

Virus Genes — Fri, 23 Sep 2011 15:51:50 +0100

Abstract Hepatopancreatic parvovirus (HPV) of shrimp is distributed worldwide and the entire genome of Thailand and Indian strains (PmDNV) and one Australian strain (PmergDNV) have now been reported. The complete nucleotide sequence of a HPV strain isolated from the fleshy prawn Fenneropenaeus chinensis in Korea (FcDNV) was determined and compared to previously reported sequences. The entire genome of FcDNV contains 6,336 nucleotides, with 40% G+C content, which is the biggest of the known HPV strains. The HPV genome has three open reading frames (ORFs) with a slight overlap between the first and second ORFs. The three ORFs encode the NS2 and NS1 proteins and VP that consist of 425, 578, and 820 amino acids, respectively. Among the three proteins, the NS1 protein shows the h...

Studies on PCV2 vaccination of 5-day-old piglets.

Clinical and Vaccine Immunology — Wed, 21 Sep 2011 04:00:00 +0100

The objective of this study was to determine the efficacy of a chimeric and a subunit PCV2 vaccine administered at 5 or 21 days of age (d). Forty-eight PCV2 naïve piglets were randomly divided into six groups of eight pigs each. Vaccination was done at d5 or d21 followed by triple challenge with PCV2, porcine parvovirus (PPV), and porcine reproductive and respiratory syndrome virus (PRRSV) at d49. Vaccinated pigs seroconverted to PCV2 approximately 14 days post vaccination and had a detectable neutralizing antibody response by 21 days post vaccination regardless of age at vaccination. At d49, the pigs vaccinated with the chimeric vaccine had significantly higher levels of neutralizing antibodies compared to the pigs vaccinated with the subunit vaccine. After challenge, vaccinated pigs had...

Immunogenicity of recombinant classic swine fever virus CD8+ T lymphocyte epitope and porcine parvovirus VP2 antigen coexpressed by Lactobacillus in swine via oral vaccination.

Clinical and Vaccine Immunology — Wed, 21 Sep 2011 04:00:00 +0100

In this study, a genetic engineering Lactobacillus coexpressing CSFV-specific cytotoxic T lymphocyte (CTL) epitope 290 and VP2 antigen of PPV was developed, and its immunopotentiating capacity as oral vaccine in pigs was analyzed. Data demonstrated that in the absence of any adjuvant, the recombinant Lactobacillus can efficiently stimulate mucosal and systemic CSFV-specific CD8(+) CTL responses to protect pigs against CSFV challenge. Moreover, anti-PPV-VP2 serum antibody IgG and mucosa antibody IgA can be induced in pigs immunized orally with the recombinant Lactobacillus, showing neutralizing effect on PPV infection. The results suggest that the recombinant Lactobacillus microecological agent may be a valuable strategy for future vaccine development against CSFV and PPV. PMID: 2194040...

Anticipating the Species Jump: Surveillance for Emerging Viral Threats

Zoonoses and Public Health — Wed, 14 Sep 2011 04:00:00 +0100

SummaryZoonotic disease surveillance is typically triggered after animal pathogens have already infected humans. Are there ways to identify high‐risk viruses before they emerge in humans? If so, then how and where can identifications be made and by what methods? These were the fundamental questions driving a workshop to examine the future of predictive surveillance for viruses that might jump from animals to infect humans. Virologists, ecologists and computational biologists from academia, federal government and non‐governmental organizations discussed opportunities as well as obstacles to the prediction of species jumps using genetic and ecological data from viruses and their hosts, vectors and reservoirs. This workshop marked an important first step towards envisioning both scientifi...

Parvovirus B19 infection associated with severe chronic aplastic anaemia—a case report

Journal of Hematopathology — Fri, 09 Sep 2011 05:49:08 +0100

We report a case of parvovirus B19-associated chronic aplastic anaemia in a 34-year-old female patient who presented with gum bleeding and ecchymotic patches over both legs and curdy vaginal discharge. She had pancytopenia on peripheral blood film examination and was subjected to bone marrow biopsy as the marrow aspirate showed peripheral blood only hence was not conclusive. Bone marrow biopsy was reported as AA, severe grade. The serological analysis performed later on revealed parvovirus B19 infection. Acute infection with B19V should be considered as a cause of acquired aplastic anaemia in individuals where other etiological factors are not elucidated. Content Type Journal ArticleCategory Case ReportPages 1-4DOI 10.1007/s12308-011-0113-9Authors Rajeev Sen, Department of Path...

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Parvovirus B19-associated purpuric–petechial eruption

Journal of Clinical Virology — Fri, 02 Sep 2011 04:00:00 +0100

Conclusions: PVB19 infection should be considered in any patient presenting with a petechial or purpuric eruption of unclear origin, and not solely for PPGSS type presentations. Therefore, we propose a simple name “PVB19-associated purpuric–petechial eruption” to describe polymorphous purpuric–petechial eruptions due to PVB19 infection, coinciding with the viremic phase of primary infection and infectivity, characterized by a self-limiting course with a benign prognosis and common histological findings. (Source: Journal of Clinical Virology)

Long-term neurodevelopmental outcome after intrauterine transfusion for fetal anemia

Early Human Development — Thu, 01 Sep 2011 04:00:00 +0100

Abstract: The long-term neurodevelopmental outcome of children born after intrauterine blood transfusion (IUT) for red cell alloimmunization is considered favorable. Severe hydrops has been identified as a strong predictor for neurodevelopmental impairment. However, the long-term outcome of survivors of IUT for congenital Parvovirus B19 infection and fetomaternal hemorrhage is not well known. Limitations of the follow-up studies to date are small sample size, lack of controls, unclear criteria for impairment and lack of standardized developmental tests. Future research should take into account more subtle impairments, since cognitive functioning (Source: Early Human Development)

F209 pain in chronic fatigue syndrome/myalgic encephalomyelitis patients: relation to active hhv-6, hhv-7 and parvovirus b19 infection/co-infection

European Journal of Pain Supplements — Thu, 01 Sep 2011 04:00:00 +0100

(Source: European Journal of Pain Supplements)

Does human bocavirus infection depend on helper viruses? A challenging case report

BioMed Central — Sun, 28 Aug 2011 23:00:00 +0100

A case of severe diarrhoea associated with synergistic human bocavirus type 1 (HBoV) and human herpes virus type 6 (HHV6) is reported. The case supports the hypotheses that HBoV infection under clinical conditions may depend on helper viruses, or that HBoV replicates by a mechanism that is atypical for parvoviruses, or that HBoV infection can be specifically treated with cidofovir. (Source: BioMed Central)

Computationally designed adeno-associated virus (AAV) Rep 78 is efficiently maintained within an adenovirus vector [Microbiology]

Proceedings of the National Academy of Sciences — Mon, 22 Aug 2011 23:00:00 +0100

Adeno-associated virus (AAV) is a single-stranded parvovirus retaining the unique capacity for site-specific integration into a transcriptionally silent region of the human genome, a characteristic requiring the functional properties of the Rep 78/68 polypeptide in conjunction with AAV terminal repeat integrating elements. Previous strategies designed to assemble these genetic elements into adenoviral (Ad) backbones have been limited by the general intolerability of AAV Rep sequences, prompting us to computationally reengineer the Rep gene by using synonymous codon pair recoding. Rep mutants generated by using de novo genome synthesis maintained the polypeptide sequence and endonuclease properties of Rep 78, while dramatically enhancing Ad replication and viral titer yields, characteristic...

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Parvovirus B19-induced Type II Mixed Cryoglobulinemia

The American Journal of Medicine — Sun, 21 Aug 2011 10:02:52 +0100

We report on a patient with type II mixed cryoglobulinemia due to acute infection with parvovirus B19 who was treated successfully with lenalidomide. (Source: The American Journal of Medicine)

Study of canine parvovirus evolution: comparative analysis of full-length VP2 gene sequences from Argentina and international field strains

Virus Genes — Fri, 19 Aug 2011 16:01:52 +0100

Abstract The continuous emergence of new strains of canine parvovirus (CPV), poorly protected by current vaccination, is a concern among breeders, veterinarians, and dog owners around the world. Therefore, the understanding of the genetic variation in emerging CPV strains is crucial for the design of disease control strategies, including vaccines. In this paper, we obtained the sequences of the full-length gene encoding for the main capsid protein (VP2) of 11 canine parvovirus type 2 (CPV-2) Argentine representative field strains, selected from a total of 75 positive samples studied in our laboratory in the last 9 years. A comparative sequence analysis was performed on 9 CPV-2c, one CPV-2a, and one CPV-2b Argentine strains with respect to international strains reported...

Bioactive Isoquinoline Alkaloids from Corydalis saxicola

Planta Medica — Thu, 18 Aug 2011 23:00:00 +0100

Planta MedDOI: 10.1055/s-0031-1280126AbstractTwelve isoquinoline alkaloids including two new nitro-containing tetrahydroprotoberberines, (−)-2,9-dihydroxyl-3,11-dimethoxy-1,10-dinitrotetrahydroprotoberberine (1) and (+)-4-nitroisoapocavidine (2), were isolated from the whole plant of Corydalis saxicola Bunting. The structures of the new compounds were established by spectroscopic analysis and chemical evidence. The inhibitory activity of these isolates against cholinesterase and canine parvovirus were evaluated. Compounds 1 and 1a, (+)-1-nitroapocavidine (5), berberine (8), palmatine (9), dehydrocavidine (10), and sanguinarine (11) showed potent inhibitory activity against acetylcholinesterase with IC50 values of less than 10 µM, while only compound 1 possessed weak activity a...

Caution in evaluation of removal of virus by filtration: Misinterpretation due to detection of viral genome fragments by PCR.

Journal of Virological Methods — Thu, 18 Aug 2011 23:00:00 +0100

Authors: Tsujikawa M, Ohkubo Y, Masuda M, Tanaka H, Takahashi K, Sasaki Y, Yunoki M, Ikuta K Abstract The testing of biological products at different stages of the manufacturing process currently involves quantitative polymerase chain reaction (Q-PCR)-based assays. Q-PCR techniques are able to detect not only the viral genome in viral particles but also fragments of degraded genome in samples. The ability of 15 and 19-nm filters to remove viruses was examined by conducting infectivity assays and Q-PCR assays using parvovirus B19 (B19), one of the smallest non-enveloped viruses. Although the filtered samples showed no infectivity, viral DNA was detected by Q-PCR. Interestingly, approximately 90% of the total viral genome in 15-nm filtrates had a detectable size of less than 0.5kb by...

Occupational risk for cytomegalovirus, but not for parvovirus B19 in child-care personnel in France

Journal of Infection — Tue, 16 Aug 2011 04:00:00 +0100

Conclusions: French female child-care staff runs an occupational risk for CMV infection, but not for B19V infection. The fraction attributable to this CMV occupational risk was not higher than the risk associated with personal factors. (Source: Journal of Infection)

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Genotype‐specific effects on left ventricular function in parvovirus B19‐positive patients with dilated cardiomyopathy

Journal of Medical Virology — Thu, 11 Aug 2011 18:52:11 +0100

AbstractGenotype‐specific effects of parvovirus B19 (B19V) infections on left ventricular function in patients with dilated cardiomyopathy (DCM) have not been investigated so far. In this prospective clinical study, the prevalences of B19V genotypes in endomyocardial biopsies from patients presenting with inflammatory heart disease and DCM were determined. A total of 139 consecutive patients were included in the study; among them 53 patients were diagnosed as DCM. Among the total study cohort, B19V DNA was detected in 65 study participants (46.8%). Genotyping of the B19V genomes in the total cohort identified genotype 1 in 38 samples (27.3%), genotype 2 in 25 samples (18.0%), and genotype 3 in only two patients (1.4%). During an average follow‐up period of 8 months left ventricular eje...

Hemiconvulsion-Hemiplegia-Epilepsy Syndrome Associated With CACNA1A S218L Mutation

Pediatric Neurology — Wed, 10 Aug 2011 22:18:48 +0100

We report on a 5-year-old Japanese girl presenting with hemiconvulsion-hemiplegia-epilepsy syndrome after infection with parvovirus B19. Magnetic resonance imaging performed 2 days after admission revealed cerebellar atrophy and marked hyperintensity in the left hemisphere on T2-weighted and diffusion-weighted imaging. Magnetic resonance angiography performed 7 days after admission demonstrated obliteration of the left proximal middle cerebral artery in the acute phase. However, this finding was not evident on brain angiography performed 25 hours after magnetic resonance angiography. Genetic analysis of familial hemiplegic migraine revealed a heterozygous S218L mutation in CACNA1A. Taken together, these results suggest that vasospasms of cerebral vascular smooth muscle, with possible corti...

Study on Clinical Characteristics and Follow-up Visit of Acquired Aplastic Anemia Associated with Parvovirus B19 Infection

Indian Journal of Pediatrics — Wed, 10 Aug 2011 15:41:42 +0100

Conclusions Parvovirus B19 infection contributes to the generation of AA, particularly in children aged 5–8 y. The AA induced may be mainly classified as serious and very serious type, with a course of disease less than 2 mo. Patients can be saved if B19-DNA is eliminated and the antibody is produced. Content Type Journal ArticlePages 1-6DOI 10.1007/s12098-011-0542-6Authors Jin-quan Wen, Children Disease Research Deparment, Xi’an Childern Hospital, Xi’an, Shanxi, People’s Republic of ChinaNan Zhou, Children Disease Research Deparment, Xi’an Childern Hospital, Xi’an, Shanxi, People’s Republic of ChinaDan Li, Children Disease Research Deparment, Xi’an Childern Hospital, Xi’an, Shanxi, People’s Republic of ChinaHai-ling Feng, Children Dise...

Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa)

Virus Genes — Sat, 06 Aug 2011 05:46:59 +0100

Abstract Novel porcine parvoviruses showing the genetic characteristics of bocaviruses have recently been identified. The first such porcine bocavirus (PoBoV1), described as boca-like virus (PBo-likeV), was discovered in PMWS affected pigs in Sweden. Later, several other bocaviruses with divergent genomes were reported under various names in domestic pigs. This is the first report of the presence of bocaviruses in European wild boars. 842 wild boar samples originating from the Western region of Romania (Transylvania) were collected during the 2006/2007 and the 2010/2011 hunting seasons and tested for the presence of PoBoV1 by polymerase chain reaction and sequencing. The results showed 12.94% (109/842) overall positivity, with an increasing prevalence from the 2006/2007 (9...

[Acute erythroblastopenia due to Parvovirus B19 revealing hereditary spherocytosis.]

Archives de Pediatrie — Tue, 02 Aug 2011 23:00:00 +0100

We report 2 observations of acute erythroblastopenia revealing hereditary spherocytosis in 2 children (1 boy and 1 girl) of non-consanguineous parents. PMID: 21820287 [PubMed - as supplied by publisher] (Source: Archives de Pediatrie)

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Human bocavirus DNA in paranasal sinus mucosa.

Emerging Infectious Diseases — Sun, 31 Jul 2011 23:00:00 +0100

Authors: Falcone V, Ridder GJ, Panning M, Bierbaum S, Neumann-Haefelin D, Huzly D To the Editor: Human bocavirus (HBoV) is a newly described parvovirus for which pathogenic potential has not clearly been elucidated (1). Recent findings suggest that HBoV may establish persistent infection of mucosal lymphocytes or contribute to tonsillar hyperplasia in children (2). In previous reports, we described prolonged HBoV DNA detection in immunocompromised children (3,4). Partial sequencing of the VP1 gene of HBoV from bronchoalveolar lavage fluid, plasma, and sphenoid sinus samples showed 100% identity, which suggested persistence of the same HBoV strain over a 5-month period (3). It remains speculative, however, whether paranasal sinus mucosa represents a site of HBoV persistence. To clarify ...

Human parvovirus 4 as potential cause of encephalitis in children, India.

Emerging Infectious Diseases — Sun, 31 Jul 2011 23:00:00 +0100

Authors: Benjamin LA, Lewthwaite P, Vasanthapuram R, Zhao G, Sharp C, Simmonds P, Wang D, Solomon T To investigate whether uncharacterized infectious agents were associated with neurologic disease, we analyzed cerebrospinal fluid specimens from 12 children with acute central nervous system infection. A high-throughput pyrosequencing screen detected human parvovirus 4 DNA in cerebrospinal fluid of 2 children with encephalitis of unknown etiology. PMID: 21801629 [PubMed - in process] (Source: Emerging Infectious Diseases)

[Human parvovirus B19 infection in sickle cell anemia patient in Mali: A case-control study.]

Archives de Pediatrie — Thu, 28 Jul 2011 23:00:00 +0100

Authors: Diallo DA, Guindo A, Dorie A, Djibo N, Algiman E, Ouane OD, Diakité AA, Traoré FF, Ag Baraika M, Dembélé AK, Tchernia G Human parvovirus B19 (HP-19) is the only Parvoviridae known to be pathogenic in human. Studies of HP-19 infection and its associated life-threatening complications in sickle cell anemia patients have been reported in Europe and the US. These results justify the development of HP-B19 prevention and strategies to reduce the incidence of severe and life-threatening complications associated with the infection in patients with sickle cell anemia, particularly in sub-Saharan Africa where the sickle cell anemia burden is high. In light of these considerations, we conducted a case-control study including 163 patients with sickle cell anemia and 163 controls. HP-B...

Constrictive Epicarditis

Journal of the American College of Cardiology — Wed, 27 Jul 2011 23:05:50 +0100

A 47-year-old male physician sought treatment for exertional dyspnea. History was remarkable for self-limiting parvovirus polyarthropathy. There was no history of radiation exposure. Physical examination was noncontributory and laboratory results were notable for past exposure to parvovirus. Chest computed tomography demonstrated a small pericardial effusion and pleural effusion. Transthoracic echocardiogram (A, Online Video 1) revealed left ventricular (LV) systolic dysfunction, ventricular interdependence, and a small organized pericardial effusion (arrow). Cardiac magnetic resonance imaging (B, Online Video 2) reconfirmed transthoracic echocardiogram findings and highlighted ventricular interdependence with septal shift (arrow). The patient underwent pericardiectomy (C), at which point ...

Serological study on parvovirus B19 infection in multitransfused thalassemia major patients and its transmission through donor units

Asian Journal of Transfusion Science — Wed, 27 Jul 2011 23:00:00 +0100

Conclusions: Most of multitransfused thalassemics were B19 seropositive or had anti-B19 IgM; in the remaining uninfected group, B19 got transmitted through infected / IgM-positive donor units. (Source: Asian Journal of Transfusion Science)

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Myocarditis as a precipitating factor for heart failure: evaluation and 1-year follow-up using cardiovascular magnetic resonance and endomyocardial biopsy

European Journal of Heart Failure — Mon, 25 Jul 2011 23:00:00 +0100

Conclusion In a Greek population with myocarditis, Chlamydia with viruses was a common finding. Cardiovascular magnetic resonance and PCR proved useful for the detection of myocarditis; EGE and LGE had the best correlation for the development of heart failure. Persistence of the initially detected infective agents was identified in patients who deteriorated further. (Source: European Journal of Heart Failure)

Human Parvovirus 4 as Potential Cause of Encephalitis in Children, India, L.A. Benjamin et al.

Emerging Infectious Diseases Journal — Mon, 25 Jul 2011 17:00:00 +0100

(Source: Emerging Infectious Diseases Journal)

The RNA profile of porcine parvovirus 4, a boca-like virus, is unique among the parvoviruses

Archives of Virology — Fri, 22 Jul 2011 17:01:29 +0100

Abstract PPV4 transcribes its genome from a single promoter, and the RNAs are generated via alternate splicing coupled with alternate polyadenylation, a strategy similar to that of the bocaviruses; however, several differences were detected. The PPV4 ORF1 codes for four NS proteins, while the bocavirus ORF1 codes for 1-3 NS proteins. Whereas the VP1/VP2 capsid proteins of bocavirus are encoded by a single RNA, VP1 and VP2 of PPV4 are encoded by two separate RNAs. While ORF3 of PPV4 encodes two NP proteins, ORF3 of bocavirus codes for only one NP polypeptide. Taken together, PPV4 is unique among the parvoviruses. Content Type Journal ArticlePages 1-8DOI 10.1007/s00705-011-1072-2Authors Andrew K. Cheung, Virus and Prion Research Unit, National Animal Disease Center, USD...

Variability of parvovirus B19 genotype 2 in plasma products with different compositions in the inactivation sensitivity by liquid‐heating

Vox Sanguinis — Thu, 21 Jul 2011 23:00:00 +0100

Conclusion Both genotypes 1 and 2 of B19 varied in sensitivity to liquid‐heating and low pH among different plasma preparations. (Source: Vox Sanguinis)

Unusual evolution of ciprofloxacin-induced hepatitis revealing a possible link with IgG4-associated autoimmune hepatitis

Digestive and Liver Disease — Wed, 13 Jul 2011 04:00:00 +0100

We report a case of a 59-year-old woman who presented a possible IgG4-associated autoimmune hepatitis trigged by liver injury due to ciprofloxacin. Although the patient was an adult female with a clinical course evocative of an autoimmune disease, the diagnosis was initially not obvious due to normal serum gammaglobulin level. This patient was seen for jaundice, asthenia and pruritus. Symptoms started few days after the end of ciprofloxacin treatment (1000mg/day for 6 days) given for a urinary tract infection. She had no personal or family medical history; she denied drinking alcohol or taking other medications; there were no clinical signs of cirrhosis. Prothrombin time was normal, total bilirubin was moderately increased whilst signs of marked cytolysis were present (). Infections with h...

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[Parvovirus B19 infection after kidney transplantation.]

Nephrologie and Therapeutique — Mon, 11 Jul 2011 23:00:00 +0100

Authors: Brodin-Sartorius A, Mekki Y, Bloquel B, Rabant M, Legendre C Prevalence for human parvovirus B19 infection is estimated to be between 2% and 30% in renal transplant recipients. In post-transplant settings, parvovirus B19 infection may occur either as a primary infection or a reactivation. Parvovirus transmission most commonly occurs through respiratory tract but may also result from graft or blood packs contamination. Co-infections with HHV-6 and CMV viruses are frequent. The hallmark symptom is anemia, more rarely pancytopenia and hemophagocytic syndrome. In respect to renal involvement, parvovirus B19 infection has been associated with graft dysfunction in 10% of cases. Both thrombotic microangiopathies and collapsing glomerulopathies have been reported concomitantly with pa...

Porcine parvovirus removal using trimer and biased hexamer peptides.

Biotechnology Journal — Sun, 10 Jul 2011 23:00:00 +0100

This study demonstrates that trimeric and hexameric peptides may have different, matrix-specific roles to play in virus removal applications. In general, the hexamer ligand may perform better for binding of specific viruses, whereas the trimer ligand may have more broadly reactive virus-binding properties. PMID: 21751387 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)

Parvovirus B19 Pre-mRNA Processing [Gene Regulation]

Journal of Biological Chemistry — Thu, 07 Jul 2011 23:00:00 +0100

We report here that efficient splicing of the B19V pre-mRNA within the first intron (upstream of the (pA)p site) stimulated the polyadenylation; in contrast, splicing of the B19V pre-mRNA within the second intron (in which the (pA)p site resides) interfered with the polyadenylation, leading to the generation of a sufficient number of B19V mRNA transcripts polyadenylated at the distal polyadenylation site ((pA)d). We also found that splicing within the second intron and polyadenylation at the (pA)p site compete during processing of the B19V pre-mRNA. Furthermore, we discovered that the U1 RNA that binds to the 5′ splice donor site of the second intron is fully responsible for inhibiting polyadenylation at the (pA)p site, whereas actual splicing, and perhaps assembly of the functional spli...

Parvovirus B19 presenting with persistent pancytopenia in a patient of T-ALL post induction chemotherapy diagnosed on bone marrow examination.

Indian Journal of Pathology and Microbiology — Fri, 01 Jul 2011 04:00:00 +0100

We report the morphological findings of parvovirus B19 infection (confirmed on serology) in a patient of T-acute lymphoblastic lymphoma (T-ALL) who underwent induction phase of chemotherapy (MCP 842 protocol). Persistent pancytopenia in the bone marrow aspirate with mild increase in blasts was thought to be due to failure to achieve marrow remission. However, giant pronormoblasts with prominent intranuclear inclusions confirmed on trephine biopsy led to the suspicion of parvovirus B19 infection which was later confirmed on serology. This case is presented to report the rarely seen classical morphological feature of parvovirus infection on bone marrow examination which was incidentally the first investigation to diagnose the viremic phase of the infection, indicating that a high index of su...

Pure Red Cell Aplasia due to B19 Parvovirus Infection after Autologous Stem Cell Transplantation

Advances in Pharmacological Sciences — Mon, 27 Jun 2011 18:25:28 +0100

We report a patient with Hodgkin's disease who developed PRCA due to parvovirus B19 after autologous SCT and who had an excellent response after treatment with gamma-globulin. (Source: Advances in Pharmacological Sciences)

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Phylogenetic analysis of porcine parvoviruses from swine samples in China

Virology Journal — Sat, 25 Jun 2011 23:00:00 +0100

Conclusions: Our study provides new molecular data on PPV stains in China, and emphasizes the importance of etiological studies of PPV in pigs.Key Wordsporcine parvovirus; phylogenetic analysis; selective pressure; molecular characterization (Source: Virology Journal)

When cats' ways of life interact with their viruses: A study in 15 natural populations of owned and unowned cats (Felis silvestris catus).

Herpes — Thu, 23 Jun 2011 23:00:00 +0100

Authors: Hellard E, Fouchet D, Santin-Janin H, Tarin B, Badol V, Coupier C, Leblanc G, Poulet H, Pontier D In natural populations, virus circulation is influenced by host behavior and physiological characteristics. Cat populations exhibit a great variability in social and spatial structure, the existence of different ways of life within a same population may also result in different epidemiological patterns. To test this hypothesis, we used a logistic regression to analyze the risk factors of Feline immunodeficiency virus (FIV), feline herpes virus (FHV), feline calicivirus (FCV), and feline parvovirus (FPV) infection in owned (fed and sheltered) and unowned (neither fed nor sheltered, unsocialized) cats living in a rural environment in the North Eastern part of France. A serological s...

A Novel Recombinant Pseudorabies Virus Expressing Parvovirus VP2 Gene: Immunogenicity and Protective Efficacy in Swine

Virology Journal — Wed, 15 Jun 2011 23:00:00 +0100

Conclusions: In this study, a recombinant PRV SA215/VP2 virus expressing PPV VP2 protein was constructed using PRV SA215 vector. The safety, immunogenicity, and protective efficacy of the recombinant virus were demonstrated in piglets and primiparous gilts. This recombinant PRV SA215/VP2 represents a suitable candidate for the development of a bivalent vaccine against both PRV and PPV infection. (Source: Virology Journal)

Seizure and Hepatosplenomegaly—Rare Manifestation of Parvovirus B-19: A Case Report and Review of the Literature

Clinical and Developmental Immunology — Wed, 01 Jun 2011 14:02:16 +0100

We present a 10 month old child with high grade fever for 10 days, generalized tonic-clonic seizure, bilateral cervical lymphadenopathy, generalized maculopapular rash, hematemesis and malena. Bone marrow aspiration and liver biopsy were done. EBV serology and parvovirus PCR were also performed. Bone marrow aspiration and biopsy showed giant pro-erythroblast consistent with parvovirus infection. PCR showed amplification of parvovirus genomic sequences. Present case highlights an atypical presentation of Parvovirus B19 infection as fever, rash and hepatosplenomegaly. (Source: Clinical and Developmental Immunology)

Association of parvovirus b19 infection with systemic lupus erythematosus: role of Th1 predominance.

J Rheumatol — Tue, 31 May 2011 23:00:00 +0100

Authors: Park SJ, Kim JH, Ha TS, Shin JI PMID: 21632693 [PubMed - in process] (Source: J Rheumatol)

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Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing.

Memorias do Instituto Oswaldo Cruz — Tue, 31 May 2011 23:00:00 +0100

Authors: Mendonça MC, Ferreira AM, Santos MG, Oviedo EC, Bello MS, Siqueira MM, Maceira JM, von Hubinger MG, Couceiro JN Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1. PMID: 21739041 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)

Primary Sjögren syndrome in a 2‐year‐old patient: role of the dentist in diagnosis and dental management with a 6‐year follow‐up

International Journal of Paediatric Dentistry — Sun, 29 May 2011 23:00:00 +0100

Conclusion. To the best of our knowledge, this is the youngest patient reported in the scientific English literature with pSS. Primary Sjögren syndrome has a wide clinical and immunologic spectrum and may progress with increased morbidity. Clinicians must be aware of the development of pSS in such an early age and exclude all possible differential findings to provide early diagnosis and treatment. (Source: International Journal of Paediatric Dentistry)

Expression of functional T-cell markers and T-cell receptor Vbeta repertoire in endomyocardial biopsies from patients presenting with acute myocarditis and dilated cardiomyopathy

European Journal of Heart Failure — Tue, 24 May 2011 23:00:00 +0100

Conclusions The T-cell infiltrates in human DCMi are characterized by differential expression of functional T-cell markers indicating Th1, Treg, and CTLs, while no major role could be confirmed for Th17. The virus-associated differential TRBV dominance suggests an antiviral specificity of virus-induced T-cell responses in human DCMi. (Source: European Journal of Heart Failure)

The combined effects of oncolytic reovirus plus Newcastle disease virus and reovirus plus parvovirus on U87 and U373 cells in vitro and in vivo

Journal of Neuro-Oncology — Mon, 23 May 2011 17:06:35 +0100

Abstract Previous results had documented oncolytic capacity of reovirus, parvovirus and Newcastle disease virus (NDV) on several tumor cell types. To test whether combinations of these viruses may increase this capacity, human U87- and U373-glioblastoma cells, in vitro or xenografted into immuno-compromised mice, were subjected to simultaneous double infections and analyzed. Our results show that reovirus (serotype-3) plus NDV (Hitcher-B1) and reovirus plus parvovirus-H1 lead to a significant increase in tumor cell killing in vitro in both cell lines (Kruskal–Wallis test, P < 0.01) and in vivo. Immunofluorescence and flow cytometry analyses demonstrated the simultaneous replication of the viruses in nearly all cells (>95%) after combined inf...

Adult human parvovirus-b19 infection presenting with hearing difficulty and dizziness.

The Tohoku Journal of Experimental Medicine — Fri, 20 May 2011 23:30:03 +0100

In conclusion, we should consider HPV-B19 infection when we evaluate patients with causeless hearing difficulty and dizziness. PMID: 21551982 [PubMed - in process] (Source: The Tohoku Journal of Experimental Medicine)

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sFlt-1 and PlGF levels in a patient with mirror syndrome related to cytomegalovirus infection

European Journal of Obstetrics, Gynecology, and Reproductive Biology — Thu, 19 May 2011 04:00:00 +0100

In 1892, John M. Ballantyne made the first description of tremendous maternal edema associated with fetal and placental hydrops due to rhesus alloimmunization . In this syndrome the mother “mirrors” the general edema presented by compromised fetus and placenta . Although the first Ballantine's report involved a patient with rhesus alloimmunization, other causes of fetal and placental hydrops have been associated with the disease, such as cytomegalovirus (CMV) and parvovirus B19 infections and twin-to-twin transfusion . The complete pathogenesis of “mirror syndrome” is still not very clear, but some authors have recently described the involvement of an anti-angiogenic state . Here, we describe a case of severe preeclampsia at 26 gestational weeks associated with massive placenta and...

Human bocavirus (HBoV) in children with respiratory tract infection by enzyme linked immunosorbent assay (ELISA) and qualitative polymerase chain reaction (PCR).

Virology Journal — Wed, 18 May 2011 23:00:00 +0100

Background: Human bocavirus (HBoV) is a recently discovered parvovirus associated with mild to severe lower respiratory tract infections in children, the aim of the work was determination of human bocavirus in nasopharyngeal aspirate (NPA) of infants by qualitative PCR and determination of acute human bocavirus infection by estimation of immunoglobulin M (IgM) antibodies in serum by enzyme linked immunosorbent assay . Results: Twenty two (22%) out of the 100 NPA specimens of the patients with respiratory manifestations were positive for HBoV by qualitative PCR, while ELISA revealed positive HBoV IgM antibodies in 18 (18%) patients who were also positive by PCR. Non of the controls were positive by both techniques .The correlation study between ELISA and PCR revealed high significant associ...

Human Parvovirus B19: General considerations and impact on patients with sickle cell disease and thalassemia as well as on blood transfusions

FEMS Immunology and Medical Microbiology — Mon, 16 May 2011 23:00:00 +0100

AbstractHuman Parvovirus B19 (B19V) is a small (22–24 nm) non‐enveloped DNA virus, belonging to the genus Erythrovirus (family Parvoviridae). Although, it causes generally self‐limiting conditions in healthy people, B19V infection could have different outcome in patients with inherited hemolytic anemias. In such high‐risk individuals, the high‐titer replication may result in bone marrow suppression triggering life‐threatening drop of the hemoglobin values (profound anemia, aplastic crisis). Nevertheless, up to day does not exist a consensus B19V screening program neither for the blood donations used in the hemotherapy, nor for high‐risk patients. Moreover, questions like molecular mechanisms by which B19V produces latency and persistent replication, primary site (sites) of ...

Human parvovirus B19: general considerations and impact on patients with sickle‐cell disease and thalassemia and on blood transfusions

FEMS Immunology and Medical Microbiology — Mon, 16 May 2011 23:00:00 +0100

AbstractHuman parvovirus B19 (B19V) is a small (22–24 nm) nonenveloped DNA virus belonging to the genus Erythrovirus (family Parvoviridae). Although it generally causes self‐limiting conditions in healthy people, B19V infection may have a different outcome in patients with inherited hemolytic anemias. In such high‐risk individuals, the high‐titer replication may result in bone marrow suppression, triggering a life‐threatening drop of hemoglobin values (profound anemia, aplastic crisis). To date there is no consensus concerning a B19V screening program either for the blood donations used in the hemotherapy or for high‐risk patients. Moreover, questions such as the molecular mechanisms by which B19V produces latency and persistent replication, the primary site (sites) of B19V i...

Current concepts in serological testing – TTID

ISBT Science Series — Sat, 14 May 2011 20:52:38 +0100

Conclusion In Germany, blood donor screening is performed using parallel serological assays (antigen and antibody detection) and by MP‐NAT for hepatitis B, hepatitis C and HIV‐1. The risk of false‐negative test results due to mutations in primer and probe binding regions is higher for NAT systems than for antibody/antigen detection tests. Therefore, the manufactures of the NAT systems are advised to improve their systems by utilising amplification in at least two conserved regions (dual‐ or triple‐targeting). The diagnostic window period for new screening strategies (e.g., antigen screening for HCV) (Source: ISBT Science Series)

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5th disease or slapped cheek disease causes a common toddler and infant rash

Blog from a Pediatrician — Sat, 14 May 2011 04:30:48 +0100

Information and photos of 5th disease, also known as slapped cheek disease, erythema infectiosum and parvovirus infection. (Source: Blog from a Pediatrician)

Parvovirus B19 infection-related acute hepatitis after rituximab-containing regimen for treatment of diffuse large B-cell lymphoma

Annals of Hematology — Mon, 02 May 2011 15:56:55 +0100

Content Type Journal ArticlePages 1-4DOI 10.1007/s00277-011-1238-8Authors Shih-Hung Yang, Department of Oncology, National Taiwan University Hospital, Yun-Lin Branch, Yunlin, TaiwanLong-Wei Lin, Department of Pathology, National Taiwan University Hospital, Yun-Lin Branch, Yunlin, TaiwanYu-Jen Fang, Department of Internal Medicine, National Taiwan University Hospital, Yun-Lin Branch, Yunlin, TaiwanAnn-Lii Cheng, Department of Oncology, National Taiwan University Hospital and National Taiwan University College of Medicine, 7, Chung-Shan South Road, Taipei, TaiwanSung-Hsin Kuo, Department of Oncology, National Taiwan University Hospital, Yun-Lin Branch, Yunlin, Taiwan Journal Annals of HematologyOnline ISSN 1432-0584Print ISSN 0939-5555 (Source: Annals of Hematology)

Increase of IL-17, IL-22 and IL-23 serum levels induced by immunoglobulin infusion for Parvovirus-B associated Pure Red Cell Aplasia in a renal transplant recipient.

Acta Oncologica — Sat, 30 Apr 2011 23:00:00 +0100

Authors: Alonci A, Penna G, Allegra A, D'Angelo A, Gangemi S, Ferraro M, Spatari G, Bacci F, Gerace D, Musolino C PMID: 21529302 [PubMed - in process] (Source: Acta Oncologica)

Detection of human herpesvirus 7 infection in young children presenting with exanthema subitum.

Memorias do Instituto Oswaldo Cruz — Sat, 30 Apr 2011 23:00:00 +0100

In conclusion, we detected HHV-7 DNA in young children from the state of Rio de Janeiro, Brazil. PMID: 21655829 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)

False-negative serology in patients with acute parvovirus B19 infection

Journal of Clinical Virology — Thu, 28 Apr 2011 23:00:00 +0100

Conclusion: Acute B19V-infection cannot be diagnosed by exclusive analysis of B19V-specific antibodies. Only the combination of assays for detection of B19V-DNA and antibodies enables correct serodiagnosis. (Source: Journal of Clinical Virology)

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Vitamin E and Selenium Levels are Within Normal Range in Pigs Diagnosed with Mulberry Heart Disease and Evidence for Viral Involvement in the Syndrome is Lacking

Transboundary and Emerging Diseases — Mon, 25 Apr 2011 14:30:56 +0100

The objectives of this study were to determine the levels of vitamin E and selenium and 13 other trace minerals in heart and liver tissues and to determine the prevalence of certain viral infections in heart tissues from MHD‐affected and MHD‐unaffected pigs and the vitamin E and selenium concentration in feed samples from selected farms with MHD. Based on the pathological examination, 114 pigs were separated into MHD lesion‐negative (L‐NEG) (n = 57) and MHD lesion‐positive (L‐POS) (n = 57) groups. Seventy‐three samples (40 L‐NEG and 33 L‐POS) were subjected to chemical analysis, and 66 (32 L‐NEG and 34 L‐POS) were subjected to PCR detection for viral pathogens. Lower (P < 0.05) levels of myocardial copper, lower (P < 0.05) levels of hepat...

Immunological arousal during acute Q fever infection

European Journal of Clinical Microbiology and Infectious Diseases — Thu, 21 Apr 2011 06:08:32 +0100

The objective of this investigation was to characterise the serological overlap between acute Q fever and other infectious and immunological diseases. We retrospectively scanned the files of patients with a positive or equivocal immunoglobulin (Ig) M for C. burnetii phase II over a period of 8 years in a general hospital. Clinical and laboratory data, including antibodies to infectious agents and antibodies related to immunological states, were recorded. Anti-nuclear antibody (ANA), smooth muscle antibody (SMA) and rheumatoid factor were positive in 38%, 33.3% and 22.2% of the cases, respectively. In patients with acute Q fever, elevated IgM levels for Epstein–Barr Virus (EBV), cytomegalovirus (CMV), Mycoplasma pneumoniae, parvovirus, Bordetella pertussis, Rickettsia conorii an...

Recombination analysis based on the complete genome of bocavirus

Virology Journal — Tue, 19 Apr 2011 23:00:00 +0100

Bocavirus include bovine parvovirus, minute virus of canine, porcine bocavirus, gorilla bocavirus, and Human bocaviruses 1-4 (HBoVs). Although recent reports showed that recombination happened in bocavirus, no systematical study investigated the recombination of bocavirus. The present study performed the phylogenetic and recombination analysis of bocavirus over the complete genomes available in GenBank. Results confirmed that recombination existed among bocavirus, including the likely inter-genotype recombination between HBoV1 and HBoV4, and intra-genotype recombination among HBoV2 variants. Moreover, it is the first report revealing the recombination that occurred between minute viruses of canine. (Source: Virology Journal)

Attempt to classify the clinical impact of DNA viruses according to the ability to activate the innate immune system

Journal of Medical Virology — Mon, 18 Apr 2011 15:57:54 +0100

AbstractThe innate immune system represents the first barrier a microbe has to meet in order to colonize and infect the host. Even in vertebrates, the adaptive immune system is polarized on the basis of what is sensed by the innate immune system. Viruses interact with cells of the innate immune system mainly via Toll‐like receptors (TLRs): in particular, unmethylated CpG dinucleotides in the genome of DNA viruses can bind TLR9 [Krug et al., 2001] in addition to KIR3DL2 [Sivori et al., 2010]. TLR‐9 binding can have stimulatory or inhibitory effects according to the exact sequence of the CpG motif [Krieg, 2002], and the cumulative effect of stimulatory and inhibitory motifs within a genome has been called the “CpG index.”Extending previous observations by other investigators [Hoelzer...

Ultrasound Diagnosis, Management and Prognosis in a Consecutive Series of 27 Cases of Fetal Hydrops following Maternal Parvovirus B19 Infection

Fetal Diagnosis and Therapy — Sat, 16 Apr 2011 06:47:17 +0100

Fetal Diagn Ther (DOI:10.1159/000323821) (Source: Fetal Diagnosis and Therapy)

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The Non-Structural Protein NS-2 of Bombyx mori Parvo-like Virus is Localized to the Nuclear Membrane.

Current Microbiology — Sun, 10 Apr 2011 23:00:00 +0100

In this study, sequence alignment results showed that NS2 shared homology with some chromosomal replication initiator protein dnaA and DNA-binding response regulators. The ns2 was cloned and expressed in E. coli, and then a polyclonal antibody of the NS2 protein was prepared successfully. The data from real-time quantitative PCR displayed that the transcription of VD1-ORF1 from BmPLV-Z-infected midguts started from 28-h post inoculation (h p.i.) in low amounts, but in high amounts at late stages of infection. Immunofluorescence showed that NS2 ultimately concentrated on the nuclear membrane in BmN cells at late stages, indicating that NS2 might be associated with integral membrane protein. PMID: 21479931 [PubMed - as supplied by publisher] (Source: Current Microbiology)

The immune enhancement of propolis adjuvant on inactivated porcine parvovirus vaccine in guinea pig.

Cellular Immunology — Fri, 08 Apr 2011 23:00:00 +0100

Authors: Ma X, Guo Z, Shen Z, Wang J, Hu Y, Wang D Two experiments were carried out. In immune response test, the immune enhancement of propolis, oilemulsion and aluminium salt were compared in guinea pig vaccinated with inactivated porcine parvovirus (PPV) vaccine. The result showed that three adjuvants could enhance antibody titer, T lymphocyte proliferation, IL-2 and IL-4 secretion of splenic lymphocyte. The action of propolis was similar to that of oilemulsion and superior to that of aluminium salt, especially in early period of vaccination propolis could accelerate antibody production. In immune protection test, the effects of three adjuvants on PPV infection were compared in guinea pig vaccinated with PPV vaccine then challenged with PPV. The result showed that propolis and oilem...

Terminal Galactose Is the Receptor for AAV9 [Microbiology]

Journal of Biological Chemistry — Thu, 07 Apr 2011 23:00:00 +0100

We report an atypical example, where desialylation increases cell surface binding and infectivity of adeno-associated virus (AAV) serotype 9, a human parvovirus isolate. Enzymatic removal of sialic acid, but not heparan sulfate or chondroitin sulfate, increased AAV9 transduction regardless of cell type. Viral binding and transduction assays on mutant Chinese hamster ovary (CHO) cell lines defective in various stages of glycan chain synthesis revealed a potential role for core glycan residues under sialic acid in AAV9 transduction. Treatment with chemical inhibitors of glycosylation and competitive inhibition studies with different lectins suggest that N-linked glycans with terminal galactosyl residues facilitate cell surface binding and transduction by AAV9. In corollary, resialylation of ...

Fractalkine in human inflammatory cardiomyopathy

Heart — Wed, 06 Apr 2011 23:00:00 +0100

Conclusion The cardiac and plasma CX3CL1/CX3CR1 system is upregulated in CMi and this affects the functional potential of PBMCs. Moreover, a direct cardiodepressive effect of CX3CL1 in cardiac tissue was demonstrated since neonatal cardiomyocytes exhibited an attenuated positive chronotropic response to β-adrenergic stimulation. (Source: Heart)

Serological Detection of Viral Infections in Captive Wild Cats from Costa Rica

Anesthesiology Research and Practice — Mon, 04 Apr 2011 14:20:26 +0100

Serum samples from a total of 44 wildcats, 28 margays (Leopardus wiedii), 10 ocelots (Leopardus pardalis), four jaguaroundis (Herpailurus yaguaroundi), one oncilla (Leopardus tigrina), and one jaguar (Panthera onca) were obtained between January 2001 and August 2002 from the Profelis Centre for rehabilitation of wild felids, located in the northwestern region of Costa Rica. Forty three samples were tested for antibodies against feline immunodeficiency virus (FIV) and p27 antigen of feline leukemia virus (FeLV), 42 samples for antibodies against feline parvovirus (FPV), and 30 for antibodies against feline calicivirus (FCV). None of the samples contained detectable antibodies against FIV or p27 antigen of FeLV, all samples contained antibodies against FPV, and one sample contained antibodie...

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Virus serology in patients with suspected myocarditis: utility or futility?

European Heart Journal — Thu, 31 Mar 2011 23:00:00 +0100

Conclusions For patients with suspected myocarditis, virus serology has no relevance for the diagnosis of myocardial infection. Endomyocardial biopsy remains the gold standard in the diagnostic of viral myocarditis. (Source: European Heart Journal)

Congenital hypoplasia of the abdominal wall muscles following fetal ascites due to parvovirus B19 infection.

The Ultrasound Review of Obstetrics and Gynecology — Thu, 31 Mar 2011 23:00:00 +0100

Authors: Macé G, Audry G, Cortey A, Nguyen A, Slaim L, Castaigne V, Garel C, Carbonne B PMID: 21433169 [PubMed - in process] (Source: The Ultrasound Review of Obstetrics and Gynecology)

Oseltamivir treatment for canine parvoviral enteritis

Advances in Small Animal Medicine and Surgery — Thu, 31 Mar 2011 23:00:00 +0100

Canine parvovirus (CPV) is a hardy, highly contagious virus that is a cause of significant disease in young dogs, despite the availability of an effective vaccine. Treatment for CPV enteritis is supportive and palliative. Multiple-directed therapies have been tried, including human recombinant granulocyte colony-stimulating factor, equine anti-endotoxin, recombinant bactericidal/permeability-increasing protein, and interferon with variable or disappointing results. (Source: Advances in Small Animal Medicine and Surgery)

Naturally occurring parvoviral infection in Hungarian broiler flocks.

Avian Pathology — Thu, 31 Mar 2011 23:00:00 +0100

The objective of the present study was to directly demonstrate the presence of the scarcely known chicken parvovirus (ChPV) and turkey parvovirus (TuPV) in Hungarian flocks experiencing clinical signs of ED. ChPV and TuPV infection were demonstrated in 15 chicken flocks and two turkey flocks, in intestinal samples collected between 2008 and 2010. The histopathological investigation revealed enteritis in the duodenum and jejunum, and atrophy of the lymphoid organs. Indirect immunohistochemistry (IHC) suggested the intestinal epithelium of chickens and turkeys as a potential replication site of the virus, similarly to other parvoviruses, while in case of the turkey samples IHC positivity was also observed in the bursa of Fabricius, liver and pancreas. However, no direct connection could be e...

A pilot study on parvovirus B19 infection in paediatric haematological malignancies.

Indian J Med Res — Thu, 31 Mar 2011 23:00:00 +0100

Authors: Kishore J, Sen M, Kumar A, Kumar A Background & objectives : Leukaemia and lymphoma are common paediatric haematological malignancies acquiring human parvovirus B19 (B19) infection. In some studies anaemia has been found in children with acute lymphoblastic leukaemia (ALL) during maintenance therapy and rarely in lymphoma. We studied frequency of B19 infection and its implications in new onset acute leukaemia (mostly ALL) and lymphoma in children. Methods: Seventy serum samples from 35 children (age <12 yr, 29 males) newly diagnosed with haematological malignancies (on induction therapy) were collected together with 34 controls (solid tumours). Children were examined clinically and for anti-B19 IgM antibodies by quantitative ELISA and B19 DNA by PCR (VP1-VP2) and nested...

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Common variable immunodeficiency and hemophagocytic features associated with a FAS gene mutation

Journal of Allergy and Clinical Immunology — Tue, 29 Mar 2011 23:00:00 +0100

We present the case of a 3-year-old boy of white nonconsanguineous parents who was referred with a history of fever, unexplained (hepato)splenomegaly, and lymphadenopathy after unexplained pneumonia. Results of microbiological tests, including serology and PCR, were negative for more than 20 viruses, including EBV, cytomegalovirus, adenovirus, parvovirus, and human herpesvirus 8. Anemia (hemoglobin, 6.0 g/dL), leukopenia (2.6 × 109/L), and thrombocytopenia (35 × 109/L) were present. Coombs test results and anti-platelet antibody levels were negative, and anti-nuclear antibody levels were positive. 18F-Fluorodeoxyglucose positron emission tomographic scanning demonstrated lymphadenopathy (including supraclavicular) and confirmed the hepatosplenomegaly. Bone marrow smears showed hypocellul...

Transfusion Medicine and the Pregnant Patient

Hematology/Oncology Clinics of North America — Tue, 29 Mar 2011 14:23:56 +0100

Alloimmunity in pregnancy is the basis for two of the major complications of pregnancy in transfusion medicine: hemolytic disease of the fetus and newborn (HDFN), and fetal and neonatal alloimmune thrombocytopenia (FNAIT). Use of Rh(D) immune globulin has dramatically reduced the incidence of HDFN in Rh(D)-mismatched pregnancies. Treatment of HDFN may involve intrauterine transfusion, with fetal and neonatal survival rates of 70% to 90%. Treatments for FNAIT include immune globulin, steroids, or in severe cases, intrauterine platelet transfusions. Transfusion medicine is central to the management of pregnancy-associated complications such as postpartum hemorrhage, parvovirus B19 infection, hemoglobinopathies, and aplastic anemia. (Source: Hematology/Oncology Clinics of North America)

In vitro evaluation of antiviral and virucidal activity of a high molecular weight hyaluronic acid

BioMed Central — Fri, 25 Mar 2011 00:00:00 +0100

Conclusions: the spectrum of the antiviral activity exhibited by HA against both RNA and DNA viruses, known to have different structures (with or without envelope) and replication strategies, suggests a non specific mechanism of action, probably involving cell membrane-virus interaction steps. The results of the kinetic experiments support this hypothesis. (Source: BioMed Central)

Model structure analysis to estimate basic immunological processes and maternal risk for parvovirus B19

Biostatistics — Tue, 22 Mar 2011 00:00:00 +0100

After a steep monotone rise with age, the seroprevalence profiles for human parvovirus B19 (PVB19) display a decrease or plateau between the ages of 20 and 40, in each of 5 European countries. We investigate whether this phenomenon is induced by waning antibodies for PVB19 and, if this is the case, whether secondary infections are plausible, or whether boosting may occur. Several immunological scenarios are tested for PVB19 by fitting different compartmental dynamic transmission models to serological data using data on social contact patterns. The social contact approach has already been shown informative to estimate transmission rates and the basic reproduction number for infections transmitted predominantly through nonsexual social contacts. Our results show that for 4 countries, model s...

Complete nucleic acid sequence of Penaeus stylirostris densovirus (PstDNV) from India.

Virus Research — Fri, 11 Mar 2011 00:00:00 +0100

Authors: Rai P, Safeena MP, Karunasagar I, Karunasagar I Infectious hypodermal and haematopoietic necrosis virus (IHHNV) of shrimp, recently been classified as Penaeus stylirostris densovirus (PstDNV). The complete nucleic acid sequence of PstDNV from India was obtained by cloning and sequencing different DNA fragment of the virus. The genome organisation of PstDNV revealed that there were three major coding domains: a left ORF (NS1) of 2001bp, a mid ORF (NS2) of 1092bp and a right ORF (VP) of 990bp. The complete genome and amino acid sequences of three proteins viz., NS1, NS2 and VP were compared with the genomes of the virus reported from Hawaii, China and the Mexico and with partial sequence available from isolates from different regions. The phylogenetic analysis of shrimp, insect ...

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The prevalence of human parvovirus B19 DNA and antibodies in blood donors from four Chinese blood centers.

Transfusion — Mon, 07 Mar 2011 00:00:00 +0100

CONCLUSIONS: Whether B19 NAT screening of blood and blood products should be launched in China, larger studies are needed to facilitate an informed decision. PMID: 21382040 [PubMed - as supplied by publisher] (Source: Transfusion)

Tenacity of mammalian viruses in the gut of leeches fed with porcine blood.

Journal of Medical Microbiology — Thu, 03 Mar 2011 00:00:00 +0100

Authors: Al-Khleif A, Roth M, Menge C, Heuser J, Baljer G, Herbst W Leech therapy is currently considered to be of high therapeutic value in medicine. However, feeding leeches with fresh animal blood during the maintenance and reproduction phase raises the anxiety of possible transmission occasionally incorporated mammalian viruses by suckling at the next host. We hypothesise that this will be abolished by subjecting leeches to quarantine measures prior to use. The required duration of quarantine will depend on the maximum survival time of pathogens in contaminated leeches. In order to be able to reliably estimate this survival time, we conducted experiments with enveloped and non-enveloped mammalian viruses possessing either RNA or DNA. Leeches were fed with porcine blood contaminated...

Immunomodulatory effect of glucan on specific and nonspecific immunity after vaccination in puppies.

Acta Veterinaria Hungarica — Tue, 01 Mar 2011 00:00:00 +0100

The objective of the study was to determine the immunostimulatory effect of β-(1,3/1,6)-D-glucan in puppies. The effect exerted on the efficacy of vaccination, especially against canine parvovirus and rabies infection, was studied. The application of vaccine and glucan leads to significant increases in the nonspecific immunological parameters (phagocytic ability of leukocytes, blastogenic response of lymphocytes, metabolic and chemotactic activity of polymorphonuclear cells). The level of antibodies against canine parvovirus (Ab CPV) and rabies infection reached the most statistically significant values on the 28th day after the application of vaccine and a syrup containing β-(1,3/1,6)-D-glucan (Group GV) as compared to the control group (Group V, puppies receiving only vaccine). Dogs wi...

Parvovirus B19 antibodies and correlates of infection in pregnant women attending an antenatal clinic in central Nigeria.

Memorias do Instituto Oswaldo Cruz — Tue, 01 Mar 2011 00:00:00 +0100

Authors: Emiasegen SE, Nimzing L, Adoga MP, Ohagenyi AY, Lekan R Human parvovirus B19 infection is associated with spontaneous abortion, hydrops foetalis, intrauterine foetal death, erythema infectiosum (5th disease), aplastic crisis and acute symmetric polyarthropathy. However, data concerning Nigerian patients with B19 infection have not been published yet. The purpose of this study was to establish the prevalence of B19 IgG and IgM antibodies, including correlates of infection, among pregnant women attending an antenatal clinic in Nigeria. Subsequent to clearance from an ethical committee, blood samples were collected between August-November 2008 from 273 pregnant women between the ages of 15-40 years who have given their informed consent and completed self-administered questionnair...

Hepatitis Associated Aplastic Anemia: A review

Virology Journal — Mon, 28 Feb 2011 00:00:00 +0100

Hepatitis-associated aplastic anemia (HAAA) is an uncommon but distinct variant of aplastic anemia in which pancytopenia appears two to three months after an acute attack of hepatitis. HAAA occurs most frequently in young male children and is lethal if leave untreated. The etiology of this syndrome is proposed to be attributed to various hepatitis and non hepatitis viruses. Several hepatitis viruses such as HAV, HBV, HCV, HDV, HEV and HGV have been associated with this set of symptoms. Viruses other than the hepatitis viruses such as parvovirus B19, Cytomegalovirus, Epstein bar virus, Transfusion Transmitted virus (TTV) and non-A-E hepatitis virus (unknown viruses) has also been documented to develop the syndrome. Considerable evidences including the clinical features, severe imbalance of ...

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Monoclonal Antibody Specific to Goose Parvovirus VP3 Protein

Hybridoma — Sun, 27 Feb 2011 02:50:10 +0100

Hybridoma Feb 2011, Vol. 30, No. 1: 101-102. (Source: Hybridoma)

Immunoglobulin G and M antibodies to human parvovirus 4 (PARV4) are frequently detected in patients with HIV-1 infection

Journal of Clinical Virology — Fri, 25 Feb 2011 00:00:00 +0100

Conclusions: PARV4 is a common viral infection in HIV-1 infected homosexuals and heterosexuals in Taiwan. The detection of IgM anti-PARV4 does not always suggest recent PARV4 infection. (Source: Journal of Clinical Virology)

Parvovirus B19 infection in pregnancy: new insights and management

Prenatal Diagnosis — Thu, 24 Feb 2011 00:00:00 +0100

AbstractIn this article, we review the virology, pathology, epidemiology and clinical spectrum of intrauterine human parvovirus B19 (B19V) infection, including intrauterine fetal death, non‐immune hydrops fetalis, thrombocytopenia and neurological manifestations such as pediatric stroke and perivascular calcifications. In addition, we discuss the new insights into the neurodevelopmental outcome of intrauterine B19V infection. Current diagnosis and management of B19V infection is summarized, including a diagnostic and follow‐up flowchart for practical clinical use. Copyright © 2011 John Wiley & Sons, Ltd. (Source: Prenatal Diagnosis)

Evolution of Canine Parvovirus in Argentina between years 2003 and 2010: CPV2c has become the predominant variant affecting the domestic dog population.

Virus Research — Thu, 24 Feb 2011 00:00:00 +0100

Authors: Calderón MG, Romanutti C, Antuono AD, Keller L, Mattion N, La Torre J The current frequency of Canine Parvovirus variants (CPV2a, CPV2b and CPV2c) in the Argentine dog population was investigated by PCR amplification of a 583bp fragment in the VP2 gene. From a total of 79 rectal swab samples that have been submitted to our laboratory since 2008, 55 (69.6%) resulted positive and were further analyzed by direct DNA sequencing. Fifty positives samples (91%) were characterized as CPV2c variant, which appeared in Argentina in the year 2003 and has been the prevalent type since 2008, whereas CPV2a and CPV2b, still found in Argentine dogs, were represented in 3.6% and 5.4% of the population, respectively. Considering that CPV2c is spreading worldwide, and that this variant is also a...

Microbial safety in xenotransplantation.

Current Opinion in Organ Transplantation — Wed, 23 Feb 2011 00:00:00 +0100

Authors: Mueller NJ, Takeuchi Y, Mattiuzzo G, Scobie L PURPOSE OF REVIEW: As clinical trials are in progress involving porcine islet cell transplantation, microbial safety remains a key issue. Therefore, in the context of pig-to-human xenotransplantation, we provide an overview of the recent progress in the studies of relevant viruses including well known problematic viruses, such as herpesviruses and porcine endogenous retroviruses (PERV) in addition to some emerging issues regarding other pathogens. RECENT FINDINGS: The ability of herpesvirus to infect across species barriers is probably underestimated and requires monitoring and control of both xenograft donors and recipients for latent infection. Exclusion from donors and recipient monitoring for other exogenous pathogens including...

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Cryoglobulinaemia and rapidly deteriorating renal function in chronic lymphocytic leukaemia

Nephrology Dialysis Transplantation — Tue, 22 Feb 2011 00:00:00 +0100

We describe a case of cryoglobulinaemic vasculitis, which manifested as purpura and rapidly deteriorating renal function in a patient with chronic lymphocytic leukaemia and coexistent parvovirus infection. This case illustrates the complex pathophysiology of cryoglobulinaemic renal injury, and suggests that infection may serve as a trigger in the presence of other pathophysiological factors. (Source: Nephrology Dialysis Transplantation)

Development of a real-time reverse-transcription-PCR method for detection of RD114 virus in canine vaccines.

Mon, 21 Feb 2011 00:00:00 +0100

In conclusion, the present investigation suggests that the newly developed method has the advantages of shorter time requirements and can be applied as a valuable screening method to detect RD114 viral RNA in vaccines. PMID: 21345697 [PubMed - as supplied by publisher] (Source: Biologicals : Journal of the International Association of Biological Standardization)

Symptomatic parvovirus B19 infection caused by blood component transfusion.

Transfusion — Fri, 18 Feb 2011 00:00:00 +0100

CONCLUSION: Vulnerability to serious B19V-related hematologic disorders depended on the patient's underlying disease state of an enhanced erythropoiesis, not on the viral load of the component transfused. To prevent clinically relevant TT-B19V, a strategy is suggested in which patients at risk of acquiring RBC aplasia or pancytopenia are targeted. PMID: 21332725 [PubMed - as supplied by publisher] (Source: Transfusion)

Parvovirus B19 infection antedating Guillain–Barre’ syndrome variant with prominent facial diplegia

Journal of Neurology — Thu, 17 Feb 2011 08:13:43 +0100

Content Type Journal ArticlePages 1-2DOI 10.1007/s00415-011-5949-5Authors Filippo Barbi, Department of Neurosciences, University of Modena and Reggio Emilia, Via P. Giardini, 1350, 41010 Modena, ItalyAlessandra Ariatti, Department of Neurosciences, University of Modena and Reggio Emilia, Via P. Giardini, 1350, 41010 Modena, ItalyKei Funakoshi, Department of Neurology, Dokkyo University, Tochigi, JapanMarisa Meacci, Laboratory Medicine, University Hospital Modena, Modena, ItalyMasaaki Odaka, Department of Neurology, Dokkyo University, Tochigi, JapanGiuliana Galassi, Department of Neurosciences, University of Modena and Reggio Emilia, Via P. Giardini, 1350, 41010 Modena, Italy Journal Journal of NeurologyOnline ISSN 1432-1459Print ISSN 0340-5354 (Source: Journal of Neurology)

Parvovirus 4 in French in‐patients: A study of hemodialysis and lung transplant cohorts

Journal of Medical Virology — Wed, 16 Feb 2011 08:12:40 +0100

This study describes the detection of PARV4 in the blood of hemodialysis and lung transplanted patients with significant difference in prevalence in these two cohorts. Further studies will be needed in order to understand better both the potential implication in host health and the natural history of this virus. J. Med. Virol. 83:717–720, 2011. © 2011 Wiley‐Liss, Inc. (Source: Journal of Medical Virology)

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Prevalence and association of human parvovirus B19V with hepatitis B and C viruses in Nigeria

Journal of Medical Virology — Wed, 16 Feb 2011 08:12:39 +0100

In this study parvovirus B19 antibodies and DNA were investigated in serum samples from 76 patients with HBV infection, 17 with HBV/HCV co‐infection and 44 healthy controls. In the sera from patients with HBV infection, anti‐B19V IgM and IgG antibodies were detected in 24/76 (32%) and 25/76 (33%), in 6/17 (35%) and 8/17 (47%) of HBV/HCV co‐infected patients, and in 14/44 (32%) and 12/44 (12%) of a non‐hepatitis healthy controls, respectively. B19V DNA was detected in 8/76 (11%) of patients with HBV infection and in 3/17 (18%) of patients with a HBV/HCV co‐infection, and in 4/44 (9%) healthy controls. The occurrence of parvovirus B19 DNA was significantly higher in patients with symptomatic HBV 4/20 (20%) compared to asymptomatic HBV carrier 4/56 (7%) (P < 0.05). Ten of the...

Viral detection in hydrops fetalis, spontaneous abortion, and unexplained fetal death in utero

Journal of Medical Virology — Wed, 16 Feb 2011 08:12:37 +0100

This study was undertaken to investigate the occurrence of viral infection in fetal death by examining tissues for the presence of DNA of several viral agents. Tissue specimens including heart, kidney, liver, lung, and placenta of 73 cases of fetal death were examined with 27 cases of elective termination of pregnancy as a control group. DNA extracted from these samples was tested for the presence of HSV, CMV, EBV, VZV, HHV‐6, HHV‐7, and PVB19. Viral DNA was found in one or more tissue samples from 25/73 cases (34%): CMV in 20, HSV in 5, parvovirus B19 in 5, HHV‐7 in 3, and HHV‐6 in 2. The presence of HHV‐6 in fetal tissue has been reported rarely. No study so far has reported the detection of HHV‐7 in fetal tissues with normal or adverse outcomes. Viral DNA was not found in an...

Early Signs of Cardiac Failure: A Clue for Parvovirus Infection Screening in the First Trimester

Fetal Diagnosis and Therapy — Tue, 15 Feb 2011 23:00:00 +0100

Fetal Diagn Ther (DOI:10.1159/000323590) (Source: Fetal Diagnosis and Therapy)

Early Signs of Cardiac Failure: A Clue for Parvovirus Infection Screening in the First Trimester?

Fetal Diagnosis and Therapy — Tue, 15 Feb 2011 10:39:35 +0100

Fetal Diagn Ther (DOI:10.1159/000323590) (Source: Fetal Diagnosis and Therapy)

Distribution of parvovirus B19 DNA in blood compartments and persistence of virus in blood donors.

Transfusion — Tue, 08 Feb 2011 00:00:00 +0100

CONCLUSIONS: The WB-to-plasma B19V DNA ratio varies by stage of infection, with 30-fold higher concentrations of B19V DNA in WB relative to plasma during the IgM-positive stage of infection followed by comparable levels during persistent infection when only IgG is present. Further study is required to determine if this is related to the presence of circulating DNA-positive RBCs derived from B19V-infected erythroblasts, B19V-specific IgM-mediated binding of virus to cells, or other factors. PMID: 21303368 [PubMed - as supplied by publisher] (Source: Transfusion)

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Mononeuropathy multiplex associated with acute parvovirus B19 infection: characteristics, treatment and outcome

Journal of Neurology — Thu, 03 Feb 2011 07:14:50 +0100

Abstract To describe the characteristics of peripheral neuropathy related to acute parvovirus B19 (B19V) infection. We reviewed clinical, electrophysiological and histological data of three patients with peripheral neuropathy and positive B19V detection (IgG, IgM and PCR) compatible with acute infection. The neuropathy fulfilled criteria for mononeuropathy multiplex (MM). It could be preceded by or concurrent with a limited purpuric eruption, but systemic manifestations were absent. The first neurological symptoms were always sensory and localized in a hand. Neuropathy was initially limited to a restricted sensory part of a nerve trunk territory. The course was subacute with successive and asymmetric injury of the limb and cranial nerves. Electromyographic study confirmed ...

Investigation of bacterial and viral agents and immune status in Behcet’s disease patients from Iran

APLAR Journal of Rheumatology — Tue, 01 Feb 2011 00:00:00 +0100

Conclusion: Although no significant association of BD was detected with a single pathogen, our findings suggest that detection of HSV DNA or Chlamydiae would rather argue against classic BD. Whether there is a discriminative potential of the tested immune mediators/receptors has to be elucidated in further studies. (Source: APLAR Journal of Rheumatology)

Fetal Parvovirus B19 Infection Presenting With Nonimmune Hydrops Fetalis

Journal of Diagnostic Medical Sonography — Wed, 26 Jan 2011 00:00:00 +0100

Parvovirus B19 is an easily transmitted virus that is common in young children but can infect gravid women if they do not have immunity. If this virus crosses the placenta and becomes a fetal infection, it can be potentially fatal to the fetus. This case report depicts the diagnosis and subsequent treatment of a fetus infected with parvovirus B19. (Source: Journal of Diagnostic Medical Sonography)

[Ballantyne syndrome caused by materno-fetal Parvovirus B19 infection: About two cases.]

Journal de Gynecologie, Obstetrique et Biologie de la Reproduction — Tue, 25 Jan 2011 00:00:00 +0100

We report two cases of Ballantyne syndrome associated with materno-fetal Parvovirus B19 infection. In the first case, the syndrome occurred at 26GW in a context of premature rupture of membranes. Parents and medical staff opted for termination of pregnancy because of the poor fetal prognosis. Maternal symptoms regressed after delivery. In the second case, the patient presented a Ballantyne's syndrome at 25GW. Intrauterine transfusions reversed symptomatology. Fetal hydrops of any etiology can be associated with this syndrome. Specific treatment of the fetus can avoid maternal complication allowing continuation of the pregnancy. PMID: 21273007 [PubMed - as supplied by publisher] (Source: Journal de Gynecologie, Obstetrique et Biologie de la Reproduction)