MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Acta Veterinaria Hungarica' source. Sat, 30 Jan 2010 16:27:41 +0100 http://www.medworm.com/index.php?rid=2978122&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19897451%26dopt%3DAbstract Authors: Jakab C, Rusvai M, Szabó Z, Szabára A, Kulka J Claudins are integral membrane proteins of the tight junction structures expressed by epithelial and endothelial cells. The present study has evaluated the expression of claudin-4 in 10 normal canine hepatoid glands and in 67 hepatoid glands with hyperplastic and neoplastic lesions. The lesions studied included normal hepatoid glands (n = 10), nodular hyperplasias (n = 10), adenomas (n = 12), epitheliomas (n = 15), differentiated carcinomas (n = 15) and anaplastic carcinomas (n = 15). There was an intensive expression of claudin-4 in normal canine hepatoid glands as well as in hyperplasias and adenomas. Claudin-4 was detected as a well-localised linear circumferential membranous staining pattern of epithelial cells (matu... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2978122 Wed, 11 Nov 2009 03:33:23 +0100 2978122 http://www.medworm.com/index.php?rid=2978121&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19897452%26dopt%3DAbstract Authors: Gál J, Jakab C, Szabó Z, Pazár P, Psáder R, Roeber F, Hegyi A, Lefler KK, Farkas B, Mándoki M A haemangioma developing in the wall of the oesophagus and protruding into its cavity is reported for the first time from a Red-eared Slider ( Trachemys scripta elegans ). As the tumour mechanically hampered swallowing, the animal was unable to eat and consequently developed a poor condition. Histopathology of the tumour revealed all characteristics of a haemangioma: the blood-filled blood-vessels having an irregular cross-section were lined with endothelial cells. Claudin-5 immunohistochemical antibodies were employed for characterising the tumour, and this examination confirmed our initial diagnosis of a haemangioma. PMID: 19897452 [PubMed - in process] ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2978121 Wed, 11 Nov 2009 03:33:19 +0100 2978121 http://www.medworm.com/index.php?rid=2978120&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19897453%26dopt%3DAbstract Authors: Jerzsele A, Nagy G The effect of various environmental factors on the stability of aqueous solutions of amoxicillin-clavulanic acid combination in a veterinary water-soluble powder product was investigated. In the swine industry, the combination is administered via the drinking water, where both substances are quickly decomposed depending on several environmental factors. The degradation rate of the substances was determined in solutions of different water hardness levels (German hardness of 2, 6 and 10) and pH values (3.0, 7.0 and 10.0), and in troughs made of different materials (metal or plastic). Increasing the water hardness decreased the stability of both substances, amoxicillin being more stable at each hardness value than clavulanate. Amoxicillin trihydrate proved to b... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2978120 Wed, 11 Nov 2009 03:33:15 +0100 2978120 http://www.medworm.com/index.php?rid=2978119&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19897454%26dopt%3DAbstract Authors: Romański KW Cholecystokinin (CCK) may affect intestinal motility, but in ruminants its precise effects have not been entirely explored. The aim of this study was to examine the effects of CCK-octapeptide (CCK-OP) on small-intestinal myoelectric activity in rams in the course of chronic experiments. Five rams underwent implantation of bipolar platinum electrodes to the duodenal bulb, distal duodenum, and jejunum as well as a strain gauge force transducer attached near the duodenal electrode. During continuous myoelectric recordings, 0.15 M NaCl or CCK-OP were injected slowly into the jugular vein. Injections of CCK-OP at doses of 20 (over 30 s), 200 (over 30 or 60 s) and 2000 (over 30, 60 or 120 s) ng/kg of body weight were each administered 5 or 30-40 min after the onset... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2978119 Wed, 11 Nov 2009 03:33:11 +0100 2978119 http://www.medworm.com/index.php?rid=2978118&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19897455%26dopt%3DAbstract Authors: Gonkowski S, Burliński P, Skobowiat C, Majewski M, Arciszewski MB, Radziszewski P, Całka J The aim of the present study was to investigate the number of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) nerve structures in the large intestine of juvenile pigs. The distribution pattern of CART-LI structures was studied by immunohistochemistry in the circular muscle layer, myenteric (MP), outer submucous (OSP) and inner submucous plexus (ISP) as well as in the mucosal layer of six regions of the large bowel: caecum, centripetal and centrifugal turns of the proximal colon, transverse colon, descending colon and rectum. CART-LI neural structures were observed in all gut fragments studied. CART-LI nerve fibres were numerous within the circular ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2978118 Wed, 11 Nov 2009 03:33:07 +0100 2978118 http://www.medworm.com/index.php?rid=2978117&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19897456%26dopt%3DAbstract Authors: Amiridis GS Despite the variety of hormonal treating regimes used, a proportion of cows with cystic ovarian disease (COD) fail to be cured. The hypothesis of this study was that cyst aspiration would improve the curing rate and/or accelerate the resumption of ovarian activity in affected cows. In four groups of cows the following treatments were administered: Group A (n = 18) only cyst aspiration, Group AGP (n = 19) cyst aspiration and a combination of GnRH and PGF 2alpha , Group GP (n = 25) only GnRH and PGF 2alpha , and Group C (n = 15) untreated control. Cysts were aspirated without ultrasonographic guidance, using a new device. All cows from Group AGP responded to treatment, while 5.5% from Group A and 16% from Group GP remained anoestrous (P < 0.05). These refractory c... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2978117 Wed, 11 Nov 2009 03:33:02 +0100 2978117 http://www.medworm.com/index.php?rid=2978116&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19897457%26dopt%3DAbstract In conclusion, slow-release melatonin applied during the non-breeding season improves testicular testosterone production but does not influence the semen characteristics and the IGF-I level of semen donor Awassi rams used in an AI programme and kept in the temperate continental zone of Europe. PMID: 19897457 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2978116 Wed, 11 Nov 2009 03:32:55 +0100 2978116 http://www.medworm.com/index.php?rid=2654333&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635707%26dopt%3DAbstract Authors: Jánosi K, Stipkovits L, Glávits R, Molnár T, Makrai L, Gyuranecz M, Varga J, Fodor L The purpose of this study was to develop and evaluate an aerosol infection method with Histophilus somni that closely resembles the natural way of infection of calves. Another aim was to compare the virulence of two H. somni strains by collecting clinical and postmortem data of experimentally infected and control animals. Seventeen conventionally reared 3-month-old calves were divided into three groups. Two groups of six animals each were exposed to suspensions containing H. somni on three consecutive days using a vaporiser mask. The third group of five animals was used as control. The data of individual clinical examination were recorded daily. All animals were exterminated, an... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654333 Thu, 30 Jul 2009 18:12:31 +0100 2654333 http://www.medworm.com/index.php?rid=2654332&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635708%26dopt%3DAbstract Authors: Sellyei B, Varga Z, Szentesi-Samu K, Kaszanyitzky E, Magyar T Pasteurella multocida causes infectious diseases in a wide range of animal species. Antimicrobial therapy is still an effective tool for treatment. Generally, P. multocida isolates are susceptible to most of the widely used commercial antimicrobial agents but their excessive and unjustified use accelerates the emergence of resistant strains. We defined the antimicrobial sensitivity pattern of 56 P. multocida strains isolated from poultry (20) and swine [16 P. multocida toxin (PMT) positive and 20 PMT negative] to 16 widely applied antibiotics (apramycin, cefquinome, chloramphenicol, colistin, doxycycline, enrofloxacin, erythromycin, florfenicol, flumequine, neomycin, oxolinic acid, penicillin, trimethoprim potentiat... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654332 Thu, 30 Jul 2009 18:12:29 +0100 2654332 http://www.medworm.com/index.php?rid=2654331&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635709%26dopt%3DAbstract Authors: Bugno M, Słota E, Pieńkowska-Schelling A, Schelling C Fluorescent in situ hybridisation (FISH) using a panel of molecular probes for all chromosome pairs obtained by chromosome microdissection of the domestic horse ( Equus caballus ) was used to diagnose karyotype abnormalities in 35 horses (32 mares, 2 stallions and 1 intersex), which were selected for the study due to infertility (23 horses), reduced fertility (10 horses) and developmental anomalies (2 horses). The use of the FISH technique with probes for each horse chromosome pair enabled the diagnosis of many different chromosome aberrations in this population. Among the horses analysed, 21 animals had normal karyotype - 64,XX (19 mares) and 64,XY (2 stallions). Fourteen animals, constituting 40% of the popula... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654331 Thu, 30 Jul 2009 18:12:26 +0100 2654331 http://www.medworm.com/index.php?rid=2654330&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635710%26dopt%3DAbstract Authors: Tapaszti Z, Forgách P, Kovágó C, Békési L, Bakonyi T, Rusvai M Microsporidiosis (nosema disease) of the European honeybee ( Apis mellifera L.) is present in bee colonies worldwide. Until recently, Nosema apis had been regarded as the causative agent of the disease, which may have many negative effects on the colony and cause heavy economic losses in apicultures. Another microsporidium species, Nosema ceranae , was reported to infest the Asian honeybee ( Apis ceranae ), but both honeybee species are susceptible to both microsporidia. In the European honeybee N. ceranae was first detected in Spain in the year 2006. As it is difficult to distinguish N. ceranae and N. apis morphologically, a rapid and accurate assay has been developed to differentiate N. a... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654330 Thu, 30 Jul 2009 18:12:24 +0100 2654330 http://www.medworm.com/index.php?rid=2654329&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635711%26dopt%3DAbstract Authors: Espino L, Suarez M, Santamarina G, Vila M, Miño N, Lopez-Peña M A 7-year-old spayed female English Cocker Spaniel was examined because of a 1-week history of lethargy, stumbling over objects and circling, and the presence of two tonic-clonic generalised seizures two days before presentation. The neurological signs suggested a lesion involving the right forebrain. Computed tomography revealed the presence of two intracranial masses, one located inside the right lateral ventricle and the other located in the right frontal lobe attached to the falx cerebri. Because of the poor prognosis, the owner refused to continue with the therapy and the dog was euthanised. On postmortem examination one mass was diagnosed histologically as a meningioma and the other as a papilloma o... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654329 Thu, 30 Jul 2009 18:12:21 +0100 2654329 http://www.medworm.com/index.php?rid=2654328&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635712%26dopt%3DAbstract The objective of our study was to vitrify Day-3 cleavage stage mouse embryos (n = 229) with the cryoloop technology using a new composition of vitrification media. Embryos were exposed to a 2-step loading of CPA, ethylene glycol (EG) and propylene glycol (PG), before being placed on the surface of a thin filmy layer formed from the vitrification solution in a small nylon loop, then they were rapidly submerged into liquid nitrogen. After warming, the CPA was diluted out from the embryos by a 3-step procedure. Survival of embryos was based on morphological appearance after thawing and continued development to expanded blastocysts upon subsequent 48-hour culture. Embryos of the two control groups were either treated likewise except that they were not vitrified, or cultured in vitro without an... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654328 Thu, 30 Jul 2009 18:12:19 +0100 2654328 http://www.medworm.com/index.php?rid=2654327&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635713%26dopt%3DAbstract In this study, the effects of a traditional, slightly modified vitrification method and vitrification using supercooled liquid nitrogen (VitMaster) applied for rabbit morula-stage embryos were compared. Embryos were equilibrated in a solution containing 1,2-propanediol (2.72 M) and glycerol (1.36 M) for 7 min and vitrified in 0.25-ml insemination straws after 1-min exposure to a vitrification solution containing additionally 1.0 M sucrose. Cooling was performed in 'normal' or supercooled liquid nitrogen. Regardless of the cooling method applied, high in vitro survival and development rates of vitrified embryos were obtained. All embryos were intact after warming, and 61 out of 65 (93.8%) and 23 out of 24 (95.8%) embryos developed to the blastocyst stage after 48-h in vitro culture of embry... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654327 Thu, 30 Jul 2009 18:12:16 +0100 2654327 http://www.medworm.com/index.php?rid=2654326&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635714%26dopt%3DAbstract Authors: Kovács K, Jakab C, Szász AM Recently, an increase in the occurrence of oral diseases in cats has been observed. Symptoms vary from case to case, but loss of appetite or fastidiousness can almost always be noted. Proliferative inflammatory eosinophilic granulomatosis is a common disease in cats, which may be localised to the skin, the mucocutaneous junctions or the oral cavity. The disease has three different manifestations: indolent cellular ulcer, eosinophilic plaque, and eosinophilic granuloma. The last mentioned form predominantly affects the medial surface of the thigh, the cheek, the tongue and the palate. Pain is not common, the lesion is nonpruritic if localised to the skin, but the nodular form in the oral cavity may make deglutition difficult. In this case, ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654326 Thu, 30 Jul 2009 18:12:14 +0100 2654326 http://www.medworm.com/index.php?rid=2654325&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635715%26dopt%3DAbstract In this study, maturation and development of the regulation of microcirculation were followed in the callus of rabbits. In the first series, the effects of vasoactive substances on blood flow velocity, perfusion pressure, duration of effects and peripheral vascular resistance of the bone marrow in the femur and tibia were compared. In the second series, the same parameters were measured in the femur and in the developing callus 10 and 15 days following gap osteotomy of the tibia. There were no significant differences between the microcirculatory reactions of the intact femur and tibia. Basal blood flow could be verified in the callus on the 10th postoperative day. No vascular reactions could be elicited. Basal blood flow velocity was higher on the 15th day, when compared to the measurement... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654325 Thu, 30 Jul 2009 18:12:11 +0100 2654325 http://www.medworm.com/index.php?rid=2654324&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635716%26dopt%3DAbstract Authors: Bálint A, Tenk M, Deim Z, Rasmussen TB, Uttenthal A, Cságola A, Tuboly T, Farsang A, Fossum C, Timmusk S, Berg M, Belák S A real-time PCR assay, based on Primer-Probe Energy Transfer (PriProET), was developed to improve the detection and quantification of porcine circovirus type 2 (PVC2). PCV2 is recognised as the essential infectious agent in post-weaning multisystemic wasting syndrome (PMWS) and has been associated with other disease syndromes such as porcine dermatitis and nephropathy syndrome (PDNS) and porcine respiratory disease complex (PRDC). Since circoviruses commonly occur in the pig populations and there is a correlation between the severity of the disease and the viral load in the organs and blood, it is important not only to detect PCV2 but also to... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654324 Thu, 30 Jul 2009 18:12:08 +0100 2654324 http://www.medworm.com/index.php?rid=2654323&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19635717%26dopt%3DAbstract Authors: Bányai K, Matthijnssens J, Szücs G, Forgách P, Erdélyi K, Ranst M, Lorusso E, Decaro N, Elia G, Martella V In rotaviruses, intragenic recombination or gene rearrangement occurs almost exclusively in the genome segments encoding for non-structural proteins. Rearranged RNA originates by mechanisms of partial sequence duplications and deletions or insertions of non-templated nucleotides. Of interest, epidemiological investigations have pointed out an unusual bias to rearrangements in genome segment 11, notably in rotavirus strains of lapine origin, as evidenced by the detection of numerous lapine strains with super-short genomic electropherotype. The sequence of the full-length genome segment 11 of two lapine strains with super-short electropherotype, LRV-4 an... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2654323 Thu, 30 Jul 2009 18:12:05 +0100 2654323 http://www.medworm.com/index.php?rid=2586124&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584033%26dopt%3DAbstract This study tested the ability of a molecular typing system based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay of the 16S rRNA gene to discriminate between the strict anaerobe genera most commonly isolated from footrot ( Bacteroides, Dichelobacter, Fusobacterium, Porphyromonas and Prevotella ) in goats in Extremadura (Spain), with a view to facilitating identification for diagnostic purposes and thus providing a useful tool for future epidemiological studies. Although the electrophoretic patterns obtained with the enzyme Tru 1I were more readily interpreted, and may thus be the best initial option, results may be confirmed by a second enzyme ( Rsa I). The PCR-RFLP assay of the 16S rRNA gene may therefore prove a useful addition to conventional bioch... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586124 Sun, 31 May 2009 23:00:00 +0100 2586124 http://www.medworm.com/index.php?rid=2586123&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584034%26dopt%3DAbstract Authors: Dudás-Györki Z, Bende B, Hetyey C, Manczur F, Reiczigel J, Vörös K The aim of the present study was to verify the validity of two-dimensional echocardiographic (2DE) measurement technique in dogs with left atrial enlargement. Thirty-one dogs with clinically and echocardiographically proven cardiac diseases were randomly selected. The left atrial right-to-left diameter ( La r-l ) and the diameter of the left atrium just above the mitral annulus ( La ama ) as well as the ratio of left atrial to aortic diameter ( La/Ao ) were measured by 2DE. The La r-l / La ama ratio was independent of body weight (R 2 = 0.0006) and age (R 2 = 0.0012), respectively. A significant linear relationship was found between La ama and La/Ao (the ratio that expresses the severity of ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586123 Sun, 31 May 2009 23:00:00 +0100 2586123 http://www.medworm.com/index.php?rid=2586122&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584035%26dopt%3DAbstract Authors: Vörös K, Hetyey C, Reiczigel J, Czirok GN The aim of the study was to establish normal reference echocardiographic values for three Hungarian dog breeds, and to determine the potential dependence of intracardiac parameters on body weight, age and gender. M-mode and two-dimensional echocardiography were performed on 95 clinically healthy dogs including 45 Hungarian Vizslas, 28 Mudis and 22 Hungarian Greyhounds. Linear intracardiac measurements included interventricular septal thickness (IVS), left ventricular internal diameter (LVID), left ventricular posterior wall thickness (LVPW) both in systole and diastole, as well as left atrial internal diameter (LAD), and aortic diameter (AOD) in early diastole. Fractional shortening (FS), end-diastolic and end-systolic left v... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586122 Sun, 31 May 2009 23:00:00 +0100 2586122 http://www.medworm.com/index.php?rid=2586121&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584036%26dopt%3DAbstract Authors: Hassanpour H, Moghaddam AK, Zarei H To clarify the effect of orally administered citric acid on the electrocardiographic parameters of pulmonary hypertensive broilers, chicks were reared at high altitude and treated with citric acid for 45 days. Increase of cardiac indices such as right ventricle/total ventricles (RV/TV), RV/body weight and TV/body weight ratio was not observed in the treated groups as compared to the control groups. Decrease of S amplitude in the treated groups (leads II, III, aVF) was only significant (P < 0.05) at 45 days of age (leads II, aVF; 1.5 g/l of citric acid). There were significant reductions of T amplitude at 28 (lead aVR) and 36 days (lead aVL) and significant reductions of R wave at 28 (lead aVL, all doses of citric acid) and 45 days of age ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586121 Sun, 31 May 2009 23:00:00 +0100 2586121 http://www.medworm.com/index.php?rid=2586107&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584037%26dopt%3DAbstract Authors: Bódi I, Nagy N, Sinka L, Igyártó BZ, Oláh I This paper introduces two novel monoclonal antibodies, designated GTr1 and GTr2, which recognise guinea fowl thrombocyte surface antigen(s). The antibodies were tested in embryos and adult birds. GTr1 and GTr2 staining emerged at embryonic days 12 and 7, respectively. After embryonic day 12 there was no difference in staining pattern between the two monoclonal antibodies. The isotype of the antibodies is IgG1. The antibodies did not react with any other haematopoietic cells of guinea fowl, and there was no species cross-reaction with chicken, turkey and quail. The antibodies can be used in interspecies chimeric and parabiotic experiments to identify cells of guinea fowl origin. PMID: 19584037 [PubMed - in proc... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586107 Sun, 31 May 2009 23:00:00 +0100 2586107 http://www.medworm.com/index.php?rid=2586106&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584038%26dopt%3DAbstract The objective of this trial was to study the effect of rancid feeds on the health status and growth of rats and to determine the pathological changes induced by dietary rancidity. Forty-two weaned male rats (body weight: 69.3 +/- 1.0 g) were divided into seven experimental groups (n = 6 each). Rats in the different groups were fed diets containing meat-and-bone meal at an inclusion rate of 19 to 22%, low or high in peroxides and high in organic acids, with or without antioxidant. The diets were isoproteic (10%) and isolipidic (6%). During the 26-day-long trial (5 days for adjustment and 21 days for the main period) the body weight gain and the feed consumption were recorded. At the end of the trial detailed gross and histopathological examinations were performed. Feeding high-peroxide feed... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586106 Sun, 31 May 2009 23:00:00 +0100 2586106 http://www.medworm.com/index.php?rid=2586105&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584039%26dopt%3DAbstract This report describes a case of a canine cutaneous grade I mast cell tumour which developed within a lipoma in the right axillar region of an 8-year-old male Boxer. Immunohistologically, the neoplastic mast cells were positive for serotonin, CD45 vimentin and p53, and negative for lysozyme, CD3 and CD79a expression. The proliferation index of the mast cell tumour based on the Ki-67 antigen was 6.1%. Between the benign neoplastic lipocytes and mastocytoma tumour cells intratumoural microvessels were detected by immunohistochemical staining using CD31 and claudin-5 as markers for vascular endothelium. PMID: 19584039 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586105 Sun, 31 May 2009 23:00:00 +0100 2586105 http://www.medworm.com/index.php?rid=2586090&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584040%26dopt%3DAbstract Authors: Gál J, Demeter Z, Palade EA, Rusvai M, Géczy C The authors describe a case of unilateral adenocarcinoma emerging from the Harderian gland, filling the right orbital cavity of a Florida Red-bellied Turtle ( Pseudemys nelsoni ). The tumour did not produce any metastasis but presented an expansive growth and led to the dislocation and protrusion of the right eyeball. Histopathological analysis revealed the presence of numerous mitotic figures in the cellular population that made up the tumour. The tumour cells completely filled the alveoli of the gland and had a nest-like structure. The authors also emphasise the importance of the differential diagnosis of this rare pathological change in turtles. Epithelial hyperplasia of the Harderian gland's duct, observed in animals... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586090 Sun, 31 May 2009 23:00:00 +0100 2586090 http://www.medworm.com/index.php?rid=2586089&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584041%26dopt%3DAbstract This study reveals that the excessive production of thrombin not only causes hypercoagulatory disorders but also exaggerates neutrophil function by the release of some enzymes which may play a destructive role during disseminated intravascular coagulation (DIC). These enzymes also inhibit anticoagulative systems, thus potentially worsening the course of the disease. PMID: 19584041 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586089 Sun, 31 May 2009 23:00:00 +0100 2586089 http://www.medworm.com/index.php?rid=2586088&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584042%26dopt%3DAbstract Authors: Rafaj RB, Matijatko V, Kis I, Kucer N, Zivicnjak T, Lemo N, Zvorc Z, Brkljacić M, Mrljak V Changes in coagulation parameters were studied in dogs naturally infected with Babesia canis canis (n = 30), and haemostasis was evaluated and compared to values obtained from healthy dogs (n = 29). To date, there have not been any studies examining the dynamics of thrombin-antithrombin complex formation in cases of canine babesiosis. Coagulation parameters evaluated before (day 0) and on days 1, 2, and 3 after treatment with imidocarb (6 mg/kg inj. s.c.) included the determination of platelet counts, the formation of thrombin-antithrombin complexes (TAT), prothrombin time (PT), activated partial thromboplastin time (APTT) and antithrombin III (AT III) activity. TAT complexes were ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586088 Sun, 31 May 2009 23:00:00 +0100 2586088 http://www.medworm.com/index.php?rid=2586087&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584043%26dopt%3DAbstract Authors: Aydin S, Gure H, Cakici H, Colakoglu S, Bircan R Arcobacter cryaerophilus was isolated from naturally infected rainbow trout ( Oncorhynchus mykiss Walbaum), and its pathogenicity was tested by intramuscular injection using healthy 1-year-old rainbow trout under cold-water conditions (at 5 degrees C). The lethal dosage of 50% end point (LD 50 ) for A. cryaerophilus was calculated as 7.79 x 10 5 viable cells. Experimental infection caused gross clinical abnormalities such as fallen scales, exophthalmia, oedema in injection region and at the base of fins, pale gills, kidney necrosis, hyperaemic areas in pale liver, haemorrhagic spots in heart, elongated spleen and swollen gallbladder. Activities of aspartate aminotransferase and alkaline phosphatase, and concentrations of glucose... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586087 Sun, 31 May 2009 23:00:00 +0100 2586087 http://www.medworm.com/index.php?rid=2586086&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584044%26dopt%3DAbstract Authors: Gyorffy A, Sayed-Ahmed A, Zsarnovszky A, Frenyó VL, Decuypere E, Bartha T Energy restriction induces changes in thyroid hormone economy in the form of a complex adaptation mechanism, in order to conserve energy storage and protein reserves. In the present work, thyroid hormone serum concentrations, hepatic deiodinase enzyme activities and hepatic deiodinase mRNA expression were examined after feed restriction and fasting. We demonstrate that during energy restriction, T 3 concentration is lowered due to a decreased T 4 activation and increased T 3 inactivation. We show that hepatic type-I deiodinase (D1) is not affected by energy restriction, however, hepatic D2 is decreased on both transcriptional and enzyme activity levels. Furthermore, hepatic D3 is increased after fee... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586086 Sun, 31 May 2009 23:00:00 +0100 2586086 http://www.medworm.com/index.php?rid=2586085&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584045%26dopt%3DAbstract Authors: Tsiligianni T, Valasi I, Cseh S, Vainas E, Faigl V, Samartzi F, Papanikolaou T, Dovolou E, Amiridis GS Follicular development and oocyte quality were assessed by laparoscopic observation and in vitro fertilisation, respectively, in melatonin-treated (Group M) and control (Group C) anoestrous Chios ewes (n = 10 in each group). Fourteen days after melatonin insertion, all ewes had laparoscopic evaluation of the follicular population followed by oocyte pick-up (OPU); on day 22 intravaginal progestagen sponges were inserted for 14 days. Two days after sponge removal the follicular population was re-evaluated and a second follicular aspiration was performed. Collected oocytes from the second OPU underwent in vitro maturation, fertilisation and culture. The number of large follicles... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586085 Sun, 31 May 2009 23:00:00 +0100 2586085 http://www.medworm.com/index.php?rid=2586084&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584046%26dopt%3DAbstract Authors: Erdélyi K, Gál J, Sugár L, Ursu K, Forgách P, Szeredi L, Steineck T Oval, firm, cutaneous tumours with a rough, hairless, pigmented surface, exhibiting a moderately pronounced papillary structure were detected on the abdominal skin of two young red deer ( Cervus elaphus ). One animal was shot in Lower Austria in 2004, the other at a deer farm in Hungary in 2007. Histological examination of both samples classified the tumours as fibropapillomas, showing marked proliferation of fibroblasts and connective tissue, accompanied by hyperkeratosis, parakeratosis and acanthosis of the overlaying epidermis, and occasional foci of inflammation. The distribution of cytokeratin and vimentin was characterised in the lesion. The presence of papillomavirus (PV) antigen was... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586084 Sun, 31 May 2009 23:00:00 +0100 2586084 http://www.medworm.com/index.php?rid=2586083&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584047%26dopt%3DAbstract Authors: Sótonyi P, Sterczer A PMID: 19584047 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2586083 Sun, 31 May 2009 23:00:00 +0100 2586083 http://www.medworm.com/index.php?rid=2474812&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457769%26dopt%3DAbstract This report describes the use of the Multilocus Sequence Typing (MLST) method for typing 32 strains previously identified as Campylobacter jejuni. This system identified a great diversity between the investigated strains. The majority of strains belonged to already existing alleles found on the Campylobacter MLST home page, and most of them represented clonal complexes ST-21 and ST-45, but there were some newly identified alleles as well. PMID: 19457769 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474812 Sun, 01 Mar 2009 05:00:00 +0100 2474812 http://www.medworm.com/index.php?rid=2474810&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457770%26dopt%3DAbstract Authors: Levkut M, Pistl J, Lauková' A, Revajová V, Herich R, Sevcíková Z, Strompfová V, Szabóová R, Kokincáková T The protective effect of Enterococcus faecium EF 55 against Salmonella enterica serovar Enteritidis phage type 4 (SE PT4) was studied in 1-day-old chicks. The EF 55 strain (isolated and characterised by the authors earlier) was applied daily (1.10(9) CFU/0.2 ml PBS) for 7 days. Oral inoculation of the SE PT4 strain was performed on day 8 in a single dose of 5.10(8) CFU/0.2 ml PBS. The experiment lasted for 21 days. Samples were collected on day 1 of the experiment to verify the absence of Salmonella, on day 8 to check colonisation of EF 55 and immunological status in experimental birds, and on days 2, 4, 6, 8 and 14 after SE PT4... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474810 Sun, 01 Mar 2009 05:00:00 +0100 2474810 http://www.medworm.com/index.php?rid=2474808&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457771%26dopt%3DAbstract This study demonstrated that real-time PCR is a rapid and reliable technique for quantifying S. Typhimurium possessing the spaQ gene in pure culture and in meat products. PMID: 19457771 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474808 Sun, 01 Mar 2009 05:00:00 +0100 2474808 http://www.medworm.com/index.php?rid=2474806&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457772%26dopt%3DAbstract Authors: Rejduch B, Knapik J, Piestrzyńska-Kajtoch A, Kozubska-Sobocińska A, Krupiński J Scrapie is an invariably fatal transmissible neurodegenerative disease of sheep, goats and moufflons, characterised by ataxia, lower body weight and changes in behaviour. The aim of this study was to determine the genotype distribution at codons 136, 154 and 171 of the PrP locus in sheep bred in Poland. The genotypes of 801 sheep representing 10 different breeds and crossbreds were analysed using real-time PCR allele discrimination method. The combination of point mutations of the three codons (136, 154 and 171) created twelve different genotypes in the PrP locus classified into five categories of risk. The highest frequency for the ARR/ARR genotype was characteristic of the Pog&#... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474806 Sun, 01 Mar 2009 05:00:00 +0100 2474806 http://www.medworm.com/index.php?rid=2474804&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457773%26dopt%3DAbstract Authors: Zhao Z, Ding J, Liu Q, Wang M, Yu J, Zhang W The immunogenicity of a DNA vaccine expressing the surface protein NcSRS2 of Neospora caninum was studied in BALB/c mice. The NcSRS2-encoding DNA was obtained by PCR amplification of the NcSRS2 ORF gene from the p43 plasmid encoding the N. caninum surface protein NcSRS2, ligated to the mammalian expression vector pcDNA3.1/Zeo(+) and propagated in E. coli DH5alpha to produce the N. caninum NcSRS2 DNA vaccine. BALB/c mice were immunised by two intramuscular injections of the DNA vaccine with or without complete Freund's adjuvant (CFA). Serum antibody titres and nitric oxide (NO) concentrations, and splenocyte proliferation and cytokine expression were measured after immunisation. The DNA vaccine induced T-cell-mediated immunity as sho... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474804 Sun, 01 Mar 2009 05:00:00 +0100 2474804 http://www.medworm.com/index.php?rid=2474802&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457774%26dopt%3DAbstract Authors: Jakab C, Szász AM, Kulka J, Baska F, Rusvai M, Gálfi P, Németh T This short report describes a case of tricuspid valvular metastasis of canine disseminated histiocytic sarcoma in a 9-year-old female Rottweiler. Immunohistochemically the malignant neoplastic cells gave a strong reaction for vimentin and lysozyme, and showed negativity for serotonin, CD3, CD79a and cytokeratin. The intratumoural microvessels were detected by immunohistochemistry using CD31 and claudin-5. This appears to be the first report of a valvular metastasis of canine malignant histiocytosis. PMID: 19457774 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474802 Sun, 01 Mar 2009 05:00:00 +0100 2474802 http://www.medworm.com/index.php?rid=2474800&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457775%26dopt%3DAbstract Authors: Gál J, Landauer K, Palade EA, Ivaskevics K, Rusvai M, Demeter Z The authors describe a squamous cell carcinoma arising from the ear canal of a Long-eared Hedgehog (Hemiechinus auritus). No metastasis could be identified elsewhere in the animal. Due to the irritation caused by the tumorous proliferation the animal constantly scratched the affected area, which led to secondary bacterial infection of the middle ear accompanied by the stagnation of an increased volume of local secretions. Using routine haematoxylin and eosin and immunohistochemical staining techniques, the tumour was identified as a squamous cell carcinoma. This work constitutes the first description of such a tumour in a Long-eared Hedgehog. PMID: 19457775 [PubMed - in process] (Source: Acta Veterinaria ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474800 Sun, 01 Mar 2009 05:00:00 +0100 2474800 http://www.medworm.com/index.php?rid=2474798&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457776%26dopt%3DAbstract In this study, T-2 + HT-2 toxin and aflatoxin B1 contamination of the diets did not affect the production traits adversely and did not exert additive effects on lipid peroxidation and on the glutathione redox system. PMID: 19457776 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474798 Sun, 01 Mar 2009 05:00:00 +0100 2474798 http://www.medworm.com/index.php?rid=2474796&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457777%26dopt%3DAbstract Authors: Rzasa J, Sechman A, Paczoska-Eliasiewicz H, Hrabia A The aim of the present investigation was to study the effect of tamoxifen (TAM), an oestrogen receptor antagonist, on the concentrations of sex hormones in chicken ovarian follicles. The experiment was carried out on Hy-line hens which were randomly divided into two groups (control and experimental). TAM was given at a dose of 4 mg/hen (per os) at first once a day for 7 consecutive days, and subsequently four times a day for the next 6 days. Control hens received placebo. Birds were killed on the day after the last TAM treatment. From the dissected ovaries the following compartments were isolated: stroma with follicles < 1 mm, white non-hierarchical (1-4 mm and 4-8 mm) and yellow hierarchical follicles (F6-F1; 18-35 mm). ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474796 Sun, 01 Mar 2009 05:00:00 +0100 2474796 http://www.medworm.com/index.php?rid=2474794&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457778%26dopt%3DAbstract Authors: Zele D, Kobal S, Vengust G, Bidovec A, Vengust A, Tavcar-Kalcher G A sensitive and reliable method for the determination of trace amounts of abamectin in muscles, kidneys and fat tissue of fallow deer is presented. Abamectin was extracted from the tissues with acetonitrile and the extract was cleaned up on a C8 solid-phase extraction cartridge. Abamectin residue was derivatised with trifluoroacetic acid anhydride and 1-methylimidazole, and determined using reversed-phase high-performance liquid chromatography under isocratic conditions and fluorescence detection. The recoveries of the method were high and consistent, ranging from 78% to 90%. The limit of detection of the method was below 1 microg/kg when analysing muscle, kidney and fat tissue. Matrix-matched calibration was u... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474794 Sun, 01 Mar 2009 05:00:00 +0100 2474794 http://www.medworm.com/index.php?rid=2474792&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457779%26dopt%3DAbstract In conclusion, the data presented in this paper suggest local production of this hormone in ovarian follicles. PMID: 19457779 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474792 Sun, 01 Mar 2009 05:00:00 +0100 2474792 http://www.medworm.com/index.php?rid=2474790&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457780%26dopt%3DAbstract In this study, the effects of OT on in vitro progesterone (P4) secretion, phosphoinositide (PI) hydrolysis and intracellular mobilisation of Ca2+ ([Ca2+]i) were investigated in porcine luteal cells during the early (days 3-5), mid(days 8-10) and late luteal phases (days 12-14) of the oestrous cycle. Basal concentrations of P4 and accumulation of inositol phosphates (IPs) were higher (P < 0.05) on days 3-5 and 8-10 of the oestrous cycle than on days 12-14. Basal [Ca2+]i mobilisation did not differ among studied periods of the oestrous cycle. Oxytocin (10(-7) M) enhanced P4 secretion and PI hydrolysis (P < 0.05) by luteal cells harvested on days 8-10 of the oestrous cycle. Moreover, OT started to increase mobilisation of [Ca2+]i at the 15th (days 3-5 and 8-10) or 30th second (days 12-1... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474790 Sun, 01 Mar 2009 05:00:00 +0100 2474790 http://www.medworm.com/index.php?rid=2474788&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457781%26dopt%3DAbstract Authors: Nemec A, Pavlica Z, Crossley DA, Zdovc I, Erzen D, Sentjurc M, Nemec M, Petelin M Nitric oxide (NO) production was investigated in the lungs, thoracic aorta, heart, liver, spleen, kidneys and brain of mice inoculated orally with Escherichia coli ATCC 25922. Detection of NO was performed by electron paramagnetic resonance (EPR) using diethyldithiocarbamate (DETC) spin trap. Nitric oxide synthase (NOS) inhibitors [nonselective: L-NAME and inducible NOS (iNOS) selective: 1400W] were used to determine the source of NO. Spin-trap only and untreated mice were included as controls. Within 2.5 hours (h) of a single oral inoculation with E. coli half of the animals had increased NO levels in all investigated organs. Thereafter the signals dropped before increasing again to reach maxima... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474788 Sun, 01 Mar 2009 05:00:00 +0100 2474788 http://www.medworm.com/index.php?rid=2474786&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457782%26dopt%3DAbstract Authors: Gyorffy A, Keresztes M, Faigl V, Frenyó VL, Kulcsár M, Gaál T, Mézes M, Zsarnovszky A, Huszenicza G, Bartha T In the regulation of energy metabolism, the liver plays an important role in the reinforcement of energy production. In periparturient cows the energy homeostasis turns into a negative energy balance that may shift the physiological regulation of energy balance towards pathological processes. Propylene glycol (PG), as a complementary source of energy used in the nutrition of dairy cows, alters systemic thyroid hormone economy; however, the exact mechanism through which highly glycogenic feed supplements impact liver metabolism is little known. Previous studies showed that only leptin receptors are expressed in the liver of cows, and now we report th... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474786 Sun, 01 Mar 2009 05:00:00 +0100 2474786 http://www.medworm.com/index.php?rid=2474784&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457783%26dopt%3DAbstract Authors: Azevedo EM, dos Santos MH, Filho CR, Freitas Neto LM, Bezerra FQ, Neves JP, Lima PF, Oliveira MA The aim of this work was to determine the ideal moment to sex goat and sheep fetuses, to compare the average time of genital tubercle (GT) migration between sexes, breeds and species, and to evaluate the accuracy of fetal sexing between sexes. A total of 317 fetuses of 219 pregnant females were monitored at 24-hour interval, from days 30 to 60 of pregnancy in ewes, and from days 40 to 60 in goats. Examinations were performed using transrectal ultrasound equipped with a linear transducer of double frequency. Fetuses were identified as male when the GT was next to the umbilical cord and female when the GT was next to the tail. The average time of GT migration in ewes (41.3 +/- 3.1 da... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474784 Sun, 01 Mar 2009 05:00:00 +0100 2474784 http://www.medworm.com/index.php?rid=2474782&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457784%26dopt%3DAbstract The objective of this study was to determine some metabolic and other factors predicting the risk of postpartum uterine disease (PUD), and the effects of puerperal metritis (PM) on metabolic status, reproduction and milk yield were analysed. A total of 105 Holstein-Friesian cows were included, and sampled on day < -14 prepartum and days 4, 10-14, 28-35 and 56-63 postpartum for metabolic tests. From day 4 the development of PUD, and from days 28-35 the ovarian activity was monitored. When grade > or = 1 + ketonuria was present on day 4 postpartum, this indicated a higher probability of PUD [odds ratio (OR) 2.64; P < 0.05] including PM occurring on days 10-14 (OR: 2.65; P < 0.05). Plasma nonesterified fatty acid (NEFA) concentrations > 0.200 mmol/l on days < -14 prepartum i... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474782 Sun, 01 Mar 2009 05:00:00 +0100 2474782 http://www.medworm.com/index.php?rid=2474780&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457785%26dopt%3DAbstract Authors: Veronesi MC, Rota A, Battocchio M, Faustini M, Mollo A Some aspects of spaying-related urinary incontinence in the bitch still remain incompletely clarified. Therefore, the aims of the present study were to evaluate the prevalence of the disease among spayed dogs, to detect differences in risk related to the type of surgery, to describe the characteristics of incontinent bitches, to assess the influence of age at surgery on the onset of incontinence occurrence, and to assess the effectiveness and long-term side effects of oestrogen therapy in affected bitches. Among 750 bitches submitted to ovariectomy or ovariohysterectomy, those showing spaying-related urinary incontinence were evaluated. Oestrogen replacement therapy consisted of administering an effective dose followed by ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474780 Sun, 01 Mar 2009 05:00:00 +0100 2474780 http://www.medworm.com/index.php?rid=2474777&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19457786%26dopt%3DAbstract Authors: Vetter J Bracken fern (Pteridium aquilinum) is the fifth most distributed common weed species of the world. Its ecological distribution is very wide, and the plant can grow and spread successfully on many types of soil. The cover of P. aquilinum is--in some cases--remarkable (e.g., in the United Kingdom). Bracken fern contains different poisonous agents: some cyanogen glycosides, factors (agents) of antithiamine character (thermolabile thiaminase and thermostable other compounds) and factors of carcinogenic activity (first of all ptaquiloside). This paper summarises and reviews different toxicological problems and poisonings caused by bracken fern in ruminants (cattle, sheep) and in non-ruminant animals (horses, pigs, rats, mice, etc.). The carcinogenic properties of the norse... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2474777 Sun, 01 Mar 2009 05:00:00 +0100 2474777 http://www.medworm.com/index.php?rid=2117105&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149098%26dopt%3DAbstract Authors: Sellyei B, Varga Z, Ivanics E, Magyar T Sixty-one avian strains of Pasteurella multocida were characterised and compared by biochemical tests, capsular PCR typing and ERIC-PCR. The strains were recovered from various avian species (goose, duck, Muscovy duck, turkey, chicken and pheasant) and represented different geographic locations in Hungary. Forty-two strains (69%) were identified as P. multocida subsp. multocida and 19 strains (31%) as P. multocida subsp. septica. The strains were grouped into 7 different biovars (1, 2, 3, 4, 5, 6 and 7). The most prevalent biovars were 1 (25%), 3 (21%) and 6 (21%). Most of the duck isolates (90%) belonged to biovar 1 or 6. The most frequent capsular type was A (93.5%). Type F represented only a small number (6.5%) of the strains. Other c... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117105 Mon, 01 Dec 2008 05:00:00 +0100 2117105 http://www.medworm.com/index.php?rid=2117104&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149099%26dopt%3DAbstract Authors: Hornok S, Meli ML, Gönczi E, Ignits E, Willi B, Lutz H, Hofmann-Lehmann R Although haemobartonellosis was previously reported in Hungary, until now the diagnosis (based on morphological identification in blood smears) has only been suggestive of the occurrence of the large species, recently reclassified as Mycoplasma haemofelis. However, in July 2007 a cat was presented at a small animal clinic with severe haemolytic anaemia, icterus and haemoglobinuria. While biochemical parameters were within the reference range, the cat had leukocytosis and rapidly decreasing haematocrit values, and eventually died 7 days after the sudden onset of aggravating clinical signs. From blood samples of the cat 'Candidatus Mycoplasma haemominutum' was identified by molecular methods, accordin... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117104 Mon, 01 Dec 2008 05:00:00 +0100 2117104 http://www.medworm.com/index.php?rid=2117103&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149100%26dopt%3DAbstract Authors: Patel JR, Bhagwat AA A real-time PCR assay was evaluated for the rapid detection (10 h) of Salmonella in meats using molecular beacon probes available as a commercial kit (iQ-Check, Bio-Rad laboratories). Raw (chicken, pork) and ready-to-eat (RTE) meats were artificially contaminated with Salmonella enterica serovar Typhimurium at the estimated level of 2 to 4 cells per 25 g. After 8 h of pre-enrichment in buffered peptone water, a molecular beacon-based PCR assay was performed to detect contamination in raw and RTE meats. The sensitivity and accuracy of the assay were compared with the conventional USDA microbiological procedure. Comparative evaluation of the USDA procedure with the rapid PCR assay for meat samples (n = 63) revealed 1 false negative pork sample with the PCR a... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117103 Mon, 01 Dec 2008 05:00:00 +0100 2117103 http://www.medworm.com/index.php?rid=2117102&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149101%26dopt%3DAbstract Authors: Bravo-Monsalvo A, Vázquez-Chagoyán JC, Gutiérrez L, Sumano H The aim of this trial was to assess the clinical efficacy of neural therapy (NT) when treating canine atopic dermatitis. Eighteen dogs (no control group), with at least a 12-month history of having nonseasonal atopic dermatitis, were included. No medication with either glucocorticoids or cyclosporin was allowed during the trial. One set of NT was given by injecting an intravenous dose of 0.1 mg/kg of a 0.7% procaine solution, followed by 10 to 25 intradermal injections of the same solution in a volume of 0.1-0.3 mL per site. Dogs were given 6-13 sets of NT during the therapy. The dermatological condition of each patient was evaluated before and after the treatment using two scales: the pruritus visual ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117102 Mon, 01 Dec 2008 05:00:00 +0100 2117102 http://www.medworm.com/index.php?rid=2117101&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149102%26dopt%3DAbstract Authors: Pákozdy A, Leschnik M, Tichy AG, Thalhammer JG In the present study, 240 cases of dogs with seizures were analysed retrospectively. The aim was to examine the underlying aetiology and to compare primary or idiopathic epilepsy (IE) with symptomatic epilepsy (SE) concerning signalment, history, ictal pattern, clinical and neurological findings. The diagnosis of symptomatic epilepsy was based on confirmed pathological changes in haematology, serum biochemistry, cerebrospinal fluid (CSF) analysis and morphological changes of the brain by CT/MRI or histopathological examination. Seizure aetiologies were classified as idiopathic epilepsy (IE, n = 115) and symptomatic epilepsy (SE, n = 125). Symptomatic epilepsy was mainly caused by intracranial neoplasia (39) and encephalitis (... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117101 Mon, 01 Dec 2008 05:00:00 +0100 2117101 http://www.medworm.com/index.php?rid=2117100&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149103%26dopt%3DAbstract Authors: Makoschey B, Bielsa JM, Oliviero L, Roy O, Pillet F, Dufe D, Valla G, Cavirani S The efficacy of an inactivated bovine respiratory syncytial virus (BRSV)--bovine parainfluenza type 3 (PI3)--Mannheimia haemolytica (Mh) combination vaccine was examined in two field studies. Calves were vaccinated (i) with the inactivated vaccine, (ii) a modified live/killed viral combination vaccine, or (iii) left unvaccinated. The efficacy of the vaccines was judged by the (i) number of treated animals, (ii) number of individual antibiotic treatments per calf and (iii) mortality rates. After vaccination with the inactivated vaccine, the number of calves requiring antibiotic treatment was significantly lower than in the unvaccinated group (odds ratios: 0.26 first study and 0.53 second study), bu... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117100 Mon, 01 Dec 2008 05:00:00 +0100 2117100 http://www.medworm.com/index.php?rid=2117099&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149104%26dopt%3DAbstract Authors: Jakab C, Halász J, Kiss A, Schaff Z, Szász AM, Rusvai M, Tóth ZA, Kulka J In our recent investigation, angiogenesis was evaluated and quantified by immunohistochemical evaluation of microvessel density (MVD) using claudin-5 (CLDN-5) as a marker for vascular endothelium in 67 canine mammary gland tumours. Computer image analysis was used to measure the intratumoural MVD. Higher intratumoural MVD was detected in malignant simple neoplasms compared with benign tumours. Furthermore, the results of MVD were correlated with histological grade, higher grades being accompanied by higher MVD. In simple adenomas and grade I tubular-tubulopapillary simple carcinomas the intratumoural microvessels were wide and regular in shape with evident erythrocytes in their lumen. In g... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117099 Mon, 01 Dec 2008 05:00:00 +0100 2117099 http://www.medworm.com/index.php?rid=2117098&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149105%26dopt%3DAbstract In conclusion, phytate in the small intestinal lumen exerted a depressive effect on the absorption of specific free amino acids from the lumen. Its depressive effect was greatest for leucine followed by glutamate, and phytate had little effect on the absorption of lysine. PMID: 19149105 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117098 Mon, 01 Dec 2008 05:00:00 +0100 2117098 http://www.medworm.com/index.php?rid=2117097&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149106%26dopt%3DAbstract Authors: Pavlik A, Jezova D, Zapletal D, Bakos J, Jelinek P The aim of the present study was to test the hypothesis that keeping laying hens in an enriched environment supposed to represent a better welfare for the birds is accompanied by decreased corticosterone levels, compared to hens kept under traditional conditions. Plasma corticosterone levels in hens reared in standard and enriched cages and those kept on deep litter from 15 to 75 weeks of age were evaluated. The highest corticosterone levels were observed in hens kept on deep litter, which was associated with a lower intensity of egg production, longest time of movement and a high percentage of time spent dustbathing and scratching. Hens housed in the enriched environment exhibited low levels of aggression, low body weight at ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117097 Mon, 01 Dec 2008 05:00:00 +0100 2117097 http://www.medworm.com/index.php?rid=2117096&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149107%26dopt%3DAbstract Authors: Molnár V, Jánoska M, Harrach B, Glávits R, Pálmai N, Rigó D, Sós E, Liptovszky M This paper describes the detection of a novel herpesvirus in a Serotine bat (Eptesicus serotinus) in Hungary. The rescued animal showed signs of icterus and anorexia and died within a day, in spite of immediate supportive therapy. Autopsy confirmed the clinical picture by the major lesions observed in the liver. Histopathology revealed vacuolar degeneration in the hepatocytes and leukocytosis in the sinusoidal lumina. By electron microscopy, hydropic degeneration and apoptotic cells with a pycnotic nucleus were found in the liver. Bacteriological examinations gave negative results. As part of a routine screening project, detection of adeno- and herpesviruses from homo... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117096 Mon, 01 Dec 2008 05:00:00 +0100 2117096 http://www.medworm.com/index.php?rid=2117095&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149108%26dopt%3DAbstract Authors: Palade EA, Biro N, Dobos-Kovács M, Demeter Z, Mándoki M, Rusvai M From a total of 1819 great tits (Parus major) ringed in 2007 in Pilis Mountains, Hungary, 15 birds presented nodular proliferative lesions on different areas of the head and eyelids, suggesting a poxvirus infection. Three birds were submitted for analysis. The presence of avipoxvirus infection was confirmed by histopathology, electron microscopy (EM) and a polymerase chain reaction (PCR) based technique. Nucleotide sequence analysis of a 428 base pairs (bp) fragment of the viral 4b core protein gene revealed 100% identity between two of the Hungarian isolates (PM9 HUN, PM33 HUN) and two great tit poxvirus strains isolated in Norway in 1973 (GTV A256, GTV A311). The third Hungarian isolate (PM34 HUN) wa... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117095 Mon, 01 Dec 2008 05:00:00 +0100 2117095 http://www.medworm.com/index.php?rid=2117094&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19149109%26dopt%3DAbstract Authors: Takács M, Dencs A, Csiszár C, Hettmann A, Rusvai E, Szomor KN, Pálfi V, Nagy B Torque teno virus (TTV) belongs to the floating genus of Anellovirus. It was discovered in a human patient, and later it was also found in animals including pigs. The aim of this study was to investigate the presence and estimate the prevalence of swine TTV in Hungarian pig herds for the first time, and to characterise the viruses found. Serum samples of 82 adult swine from 13 piggeries and 44 weaned pigs from one large herd were tested by PCR for the presence of TTV DNA. Viral DNA was found in 30% of the adult swine and 73% of the weaned pigs tested. Liver and intestine of weaned pigs were also tested and found to be infected at a lower rate. The TTV sequences found in sera and intes... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=2117094 Mon, 01 Dec 2008 05:00:00 +0100 2117094 http://www.medworm.com/index.php?rid=1848590&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828479%26dopt%3DAbstract Authors: Makrai L, Dénes B, Hajtós I, Fodor L, Varga J Two hundred and twelve Rhodococcus equi strains were isolated from soil, nasal and rectal swabs of horses and immunocompromised human patients in Hungary and serotyped using Prescott's serotyping system. One hundred and forty-seven strains (69.3%) belonged to serotype 1, 22 strains (10.4%) to serotype 2, 6 strains (2.8%) to serotype 3 and 1 strain (0.5%) to serotype 4. Serotypes 5, 6 and 7 were not found and 36 strains (17%) could not be typed. Serotype 1 (72%) was the type most commonly isolated from clinical samples of foals or from the soil of horse facilities. Six out of 8 R. equi strains from humans belonged to serotype 2, and two human strains were untypable. The data show that the prevalence of R. equi serotypes va... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848590 Mon, 01 Sep 2008 04:00:00 +0100 1848590 http://www.medworm.com/index.php?rid=1848589&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828480%26dopt%3DAbstract This study was designed to determine the presence and the prevalence of Anaplasma phagocytophilum infection in sheep and cattle in the Middle and Eastern Black Sea Regions of Turkey in which the potential vector, Ixodes ricinus, is widespread. Blood samples were collected from 720 sheep and 720 cattle from 6 provinces of the region, and used for detecting antibodies to A. phagocytophilum by indirect immunofluorescent antibody test (IFAT) and specific nucleic acids by a nested polymerase chain reaction (PCR). Blood smears were also prepared and examined microscopically for the presence of A. phagocytophilum-like organisms in polymorphonuclear cells. Of the animals examined, antibodies were detected in 110 (15.27%) cattle and 107 (14.86%) sheep and A. phagocytophilum-like organisms were dete... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848589 Mon, 01 Sep 2008 04:00:00 +0100 1848589 http://www.medworm.com/index.php?rid=1848588&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828481%26dopt%3DAbstract The objective of this work was to evaluate the long-term efficacy of a proposed therapeutic protocol in 12 dogs with a tentative diagnosis of degenerative myelopathy, followed-up for a 6-month period. Twelve dogs fulfilling the antemortem inclusion criteria (breed, age, adequate vaccination, history of progressive posterior ataxia and/or paraparesis, no radiographic and myelographic abnormalities in the spinal cord and vertebral column) were allocated. All these dogs presented signs of thoracolumbar syndrome (T3-L3), scored as grade I (mild to moderate ataxia and paraparesis) in 10 and grade II (severe ataxia and ambulatory paraparesis) in 2 cases. Treatment included the use of epsilon-aminocaproic acid and N-acetylcysteine, supplemented with vitamins B, C and E. Prednisolone was given for... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848588 Mon, 01 Sep 2008 04:00:00 +0100 1848588 http://www.medworm.com/index.php?rid=1848587&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828482%26dopt%3DAbstract Authors: Ginja MM, Silvestre AM, Ferreira AJ, Gonzalo-Orden JM, Orden MA, Melo-Pinto P, Llorens-Pena MP, Colaço J Two hundred and fifteen Estrela Mountain Dogs (EMD) were examined using the PennHIP method between 2002 and 2006. Passive hip laxity (PHL) was estimated calculating the distraction index (DI). Pedigree information was obtained from the Portuguese Kennel Club. The heritability and breeding values were estimated using the linear Animal Model. The DI, using as reference the worst joint of each animal, ranged from 0.15 to 1.12 (0.55 +/- 0.19). The PHL was equal in right and left sides, and was higher in females than in males (P > 0.05 and P <0.01 in the t-test, respectively). The estimated heritability was 0.83 +/- 0.11. The mean breeding values for PHL were stable i... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848587 Mon, 01 Sep 2008 04:00:00 +0100 1848587 http://www.medworm.com/index.php?rid=1848586&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828483%26dopt%3DAbstract Authors: Romvári R, Szabó A, Andrássy G, Petrási Z, Donkó T, Horn P A two-way selection procedure was performed on a Landrace pig line. First the cardiac output (CO) values (6.89 +/- 1.27 L/min) were estimated by ECG-gated magnetic resonance imaging (MRI) to quantify the heart performance of the boars (n = 30), then total body skeletal muscle volume (MVTB; 48.7 +/- 5.17 dm3) was determined applying spiral computerised tomography (CT). The relative cardiac output value (RCO) was developed, expressing the relationship between CO and MVTB. Four superior [RCO value below 6.7 dm3 / (L x min(-1))] and one minus variant [(8.97 dm3 / (L x min(-1))] boars were taken into breeding. The best fifteen percentage of the first generation males (n = 30) was characterised accor... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848586 Mon, 01 Sep 2008 04:00:00 +0100 1848586 http://www.medworm.com/index.php?rid=1848585&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828484%26dopt%3DAbstract Authors: Zidek R, Jakabová D, Trandzík J, Buleca J, Jakab F, Massányi P, Zöldág L Genetic variability and relationships among five cattle breeds (Holstein, Pinzgau, Limousin, Slovak Spotted and Charolais) bred in the Slovak Republic were investigated separately using 11 microsatellite markers and 61 blood group systems. Allele frequency, heterozygosity (Ho, HE) and PIC values were investigated. F-statistics were computed separately. For microsatellite markers FIS, FIT, FST and for blood groups HS, HT, GST parameters were calculated. Microsatellite and blood group comparison showed similar results by F-statistics but some differences were marked using the other methods. Both methods were able to detect close relation between Slovak Pinzgau and Slovak Spotted cat... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848585 Mon, 01 Sep 2008 04:00:00 +0100 1848585 http://www.medworm.com/index.php?rid=1848584&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828485%26dopt%3DAbstract Authors: Nikolić A, Dimitrijević S, Katić-Radivojević S, Klun I, Bobrć B, Djurković-Djaković O To identify areas of risk for canine-related zoonoses in Serbia, the aim of this study was to provide baseline knowledge about intestinal parasites in 151 dogs (65 household pets, 75 stray and 11 military working dogs) from Belgrade. The following parasites, with their respective prevalences, were detected: Giardia duodenalis (14.6%), Ancylostomatidae (24.5%), Toxocara canis (30.5%), Trichuris vulpis (47.0%) and Taenia-type helminths (6.6%). Of all examined dogs, 75.5% (114/151) were found to harbour at least one parasite species. Of these, mixed infections with up to four species per dog occurred in 44.7% (51/114). Infections with all detected specie... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848584 Mon, 01 Sep 2008 04:00:00 +0100 1848584 http://www.medworm.com/index.php?rid=1848583&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828486%26dopt%3DAbstract Authors: Jakab C, Halász J, Szász AM, Batmunkh E, Kiss A, Schaff Z, Rusvai M, Gálfi P, Kulka J The recently identified claudins are dominant components of tight junctions, responsible for cell adhesion, polarity and paracellular permeability. Certain claudins have been shown to have relevance in tumour development. The aim of the present study was to analyse the expression of claudin-1, -2, -3, -4, -5, -7 and -10 in normal canine mammary glands. Samples from the inguinal mammary regions of 20 non-castrated, 1-13 years old female dogs were studied. Immunohistochemical analysis was performed on conventional specimens and tissue microarrays. The results of the immunohistochemical reactions detecting claudins in tissue sections were photodocumented. The immunoreactivity of c... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848583 Mon, 01 Sep 2008 04:00:00 +0100 1848583 http://www.medworm.com/index.php?rid=1848582&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828487%26dopt%3DAbstract Authors: Szeredi L, Tenk M, Jánosi S, Pálfi V, Hotzel H, Sachse K, Pospischil A, Bozsó M, Glávits R, Molnár T Cases of equine abortion and perinatal foal losses were investigated in Hungary during a three-year period (1998-2000). Samples from aborted equine fetuses and newborn foals (total n = 96) were examined using bacteriological, virological, pathological, immunohistochemical (IHC), molecular biological and serological methods. The cause of abortion and perinatal foal loss was identified in 67/96 cases (70%); viral infection was found in 22 (23%), viral and bacterial coinfection in 1 (1%), bacterial infection in 23 (24%), protozoan infection in 1 (1%) and fungal infection in 2 cases (2%). Morphological lesions suggestive of infection were recorded in 2 (2%)... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848582 Mon, 01 Sep 2008 04:00:00 +0100 1848582 http://www.medworm.com/index.php?rid=1848581&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828488%26dopt%3DAbstract Authors: Schwarz T, Kopyra M, Nowicki J Follicular growth after antrum formation is determined by follicle-stimulating hormone (FSH). Only two ways are possible for recruited follicles, continuing development or atresia. In gilts, intensive ovarian follicular growth begins between 60 and 100 days of age, and fluctuations of the ovarian morphological status last about 20 days; however, at that time there are no really large follicles. Final follicular development is under luteinising hormone (LH) control; this is why the attainment of puberty is related to an increase in serum oestradiol to a level that causes a preovulatory surge of this gonadotropin. The pool of follicles at the beginning of the oestrous cycle is about 30-40, most of which are small (< 3 mm) and growing. Then, the ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848581 Mon, 01 Sep 2008 04:00:00 +0100 1848581 http://www.medworm.com/index.php?rid=1848580&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828489%26dopt%3DAbstract Authors: Opalka M, Kugla-Owczarska J, Kaminska B, Puchajda-Skowronska H, Hryniewicka W, Dusza L The aim of this study was to investigate how long-term feeding of ganders with diets containing different levels of phytoestrogens affects the morphometry of testes, plasma steroid levels, sperm parameters and reproductive performance. Male Bilgoraj geese were fed diets containing grass meal (with low phytoestrogen levels) and those containing alfalfa meal and soybean meal (with higher levels of phytoestrogens). Testes were obtained from ganders at the peak of mating and the ongoing laying period as well as at the beginning of photorefractoriness. The morphometric parameters were measured in frozen testicular sections. Blood was collected monthly from December to June, and plasma testosteron... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848580 Mon, 01 Sep 2008 04:00:00 +0100 1848580 http://www.medworm.com/index.php?rid=1848579&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828490%26dopt%3DAbstract Authors: Tsiligianni T, Ntovolou E, Amiridis GS The purpose of the present study was to investigate the feasibility of improving the synchronisation of lambing after oestrus synchronisation and artificial insemination (AI). To this end, low doses of dexamethasone 21-isonicotinate (DEX) alone or in combination with prostaglandin F2a (PG) were used in five treated groups (n = 20 each) and one control group (n = 136) of Chios ewes. On day 143 of pregnancy 1.5 mg DEX was given in Group 5, while on day 146 the following treatments were applied: 0.0375 mg PG in Groups 4 and 5, and 1, 1.5 and 2 mg of DEX in ewes of Groups 1, 2 and 3, respectively. The control ewes received no treatment. The 1.5 and 2 mg dose of DEX was more effective in synchronising labour as regards the treatment to lambing... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848579 Mon, 01 Sep 2008 04:00:00 +0100 1848579 http://www.medworm.com/index.php?rid=1848578&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828491%26dopt%3DAbstract Authors: Varga E, Gajdócsi E, Makkosné BP, Salamon I, Bali Papp A The breeding of Mangalica, a native pig breed in Hungary, had been started in 1833, but this pig breed almost became extinct in Hungary in the past decades. In 1991, the number of sows was only 200. Although in these days the existing Mangalica population consists of more than 6000 animals representing different colour variations, the preservation of this traditional pig breed is still very important. Vitrification is a potential tool for the preservation of gametes and embryos of these animals. The aim of this study was to investigate the effects of vitrification on the developmental competence of Mangalica (M) and Large White (LW) oocytes following fertilisation. The oocytes were vitrified by the Open Pulled ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848578 Mon, 01 Sep 2008 04:00:00 +0100 1848578 http://www.medworm.com/index.php?rid=1848577&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828492%26dopt%3DAbstract In conclusion, the autologous graft presents acceptable long-term patency rate. It is easy to handle and the concept of beneficial presence of the anti-clot mesothelium until endothelialisation seems to work. The first clinical use was already reported by our group with more than 2 years survival. PMID: 18828492 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848577 Mon, 01 Sep 2008 04:00:00 +0100 1848577 http://www.medworm.com/index.php?rid=1848576&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18828493%26dopt%3DAbstract Authors: Cságola A, Cadar D, Tuboly T Little information is known about infection, replication and transmission of porcine circovirus type 2 (PCV2) in species other than swine. Two sets of animal experiments were carried out to investigate the susceptibility of mice to PCV2 and to study their possible role in maintaining and transmitting the virus. In the first experiment 14 mice were inoculated with PCV2 by the intraperitoneal route with 5 x 10(2) TCID50 of the PCV2-ROM strain (Cadar et al., 2007). In a second experiment 24 mice were divided into two groups (A and B); mice in Group A (n = 18) were inoculated orally with 1 x 10(5) TCID50 PCV2-ROM and mice in Group B (n = 6) were left uninoculated until day 12 post inoculation (p.i.), when they were mixed with Group A. The animals ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1848576 Mon, 01 Sep 2008 04:00:00 +0100 1848576 http://www.medworm.com/index.php?rid=1675761&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669241%26dopt%3DAbstract Authors: Zanetti S, Bua A, Molicotti P, Delogu G, Mura A, Ortu S, Sechi LA During a six-month period a region of Northern Sardinia was monitored to check the presence of mycobacterial infections in wild boars. Forty-eight serum and 229 biopsy samples were collected from different animals and examined by both traditional diagnostic techniques (culture, bacterioscopic and molecular tests) and enzyme-linked immunosorbent assay (ELISA). The latter was used to determine the antibody response against both methylated and nonmethylated Heparin-Binding Haemagglutinin (HBHA) protein. Nine mycobacterial strains were isolated: three M. avium ssp. paratuberculosis (Map), three M. avium, one M. interjectum and two M. scrofulaceum strains. By PCR, only one animal was positive for M. bovis, whereas 10... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675761 Sun, 01 Jun 2008 04:00:00 +0100 1675761 http://www.medworm.com/index.php?rid=1675760&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669242%26dopt%3DAbstract Authors: Schäfer T, Merkl J, Klemm E, Wichmann HE, Ring J The study of allergies in creatures living together without being relatives can help us understand the impact of environmental influences. We tested the association of allergies in humans and their pets. A nested unmatched case-control study was performed in a random sample of 4261 inhabitants, aged 25-74 years, of the City of Augsburg, Germany and two adjacent counties. Using standardised computer-assisted face-to-face interviews, we determined and compared the prevalence of doctor-diagnosed atopic diseases (hay fever, asthma, atopic dermatitis) in the study subjects and veterinarian-diagnosed allergies in their pets. Pets were kept in 48.0% of the households (cats 26.1%, dogs 20.1%, rodents 9.7%) and a veterinarian had di... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675760 Sun, 01 Jun 2008 04:00:00 +0100 1675760 http://www.medworm.com/index.php?rid=1675759&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669243%26dopt%3DAbstract Authors: Goicoa A, Espino L, Rodriguez I, Puigdemont A, Brazis P, Rejas J Sensitisation to mites is frequent in atopic dogs. The main mite genus involved in canine atopic dermatitis is Dermatophagoides. The importance of storage mite allergens in dogs has been controversial. The aim of this study was to evaluate the sensitisation rates against storage mites (Lepidoglyphus destructor and Tyrophagus putrescentiae) and house dust mites (Dermatophagoides farinae and D. pteronyssinus) in atopic dogs from Galicia, a highly humid and temperate region of Spain, using a FcepsilonRIalpha-based immunoglobulin E (IgE) in vitro test. The study was performed on 95 dogs suffering from atopic dermatitis and presenting detectable specific serum IgE levels: 91.6% of the dogs tested positive for storage ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675759 Sun, 01 Jun 2008 04:00:00 +0100 1675759 http://www.medworm.com/index.php?rid=1675758&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669244%26dopt%3DAbstract Authors: Fésüs L, Zsolnai A, Anton I, Sáfár L The first results of the Hungarian sheep prion protein (PrP) genotyping programme are discussed in this paper. To obtain initial genotype frequency data 10 commercial (Hungarian Merino, German Mutton Merino, Merino Landschaf, German Blackheaded, Suffolk, Texel, Ile de France, Charollais, Lacaune, British Milksheep) and 4 indigenous (Gyimes Racka, Hortobágy Racka, Tsigaja, Cikta) breeds were sampled in 2003 and 2004, and the PrP genotypes were determined by microsequencing analysis with capillary electrophoresis. In all commercial breeds, a higher number of sheep were genotyped in 2005 (3648) and in 2006 (3834) within the breeding programme to increase scrapie resistance, and the estimated frequency data were compare... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675758 Sun, 01 Jun 2008 04:00:00 +0100 1675758 http://www.medworm.com/index.php?rid=1675757&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669245%26dopt%3DAbstract The objective of this study was to estimate the effect of the thyroglobulin (TG) locus on beef quality traits in some beef cattle breeds and to investigate the effect of the DGAT1 locus on milk production traits in the Hungarian Holstein Friesian population. TG and DGAT1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. At the TG locus TT bulls showed the highest fat percentage values in the longissimus dorsi muscle (m. longissimus dorsi); the difference between CC and TT genotypes was significant. DGAT1 GC/GC cows had the highest milk, fat and protein yield values. Due to the relatively small number of GC/GC cows the difference proved to be significant only between AA/AA and AA/GC genotypes. PMID: 18669245 [PubMed - in pr... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675757 Sun, 01 Jun 2008 04:00:00 +0100 1675757 http://www.medworm.com/index.php?rid=1675756&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669246%26dopt%3DAbstract This study revealed that neutrophils under the influence of TNFalpha exacerbated the release of elastase, MPO, ALKP and superoxide. IL-8, in turn, triggered those neutrophils which were isolated from heifers suffering from BRD, and released elastase, ALKP and 5-oxo-ETE, but not MPO or superoxide (O2(-)). The most pronounced degranulative response was observed in the acute form of BRD and a lesser response was seen in the chronic form. The secretory action of neutrophils varied not only depending on the form and phase of the disease but it also diminished successively during recovery. PMID: 18669246 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675756 Sun, 01 Jun 2008 04:00:00 +0100 1675756 http://www.medworm.com/index.php?rid=1675755&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669247%26dopt%3DAbstract Authors: Nourollahi-Fard SR, Woo PT The aims of the present study were to determine (a) the effectiveness of an attenuated live Cryptobia salmositica vaccine; (b) the effects of food deprivation on the immune response and its duration in rainbow trout (Oncorhynchus mykiss) immunised with a live C. salmositica vaccine or with a killed Aeromonas salmonicida vaccine. The fish were divided into three groups (I, II and III; 14 fish per group), those in Groups I and II were under food deprivation (0.40% of body weight), while Group III fish were fed to satiety. The study showed that the attenuated strain of C. salmositica did not cause anaemia and disease, and the fish were protected from clinical disease when they were challenged with virulent parasites. Parasitaemia in all fish vaccinated ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675755 Sun, 01 Jun 2008 04:00:00 +0100 1675755 http://www.medworm.com/index.php?rid=1675754&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669248%26dopt%3DAbstract Authors: Szeredi L, Szentirmai C A postweaning pig died in spite of antibiotic therapy showing wasting in a small herd. Postweaning multisystemic wasting syndrome (PMWS) was diagnosed on the basis of gross pathological and histological lesions and the presence of moderate amounts of porcine circovirus 2 (PCV2) antigen in tissue samples. Mycotic gastritis caused by Zygomycetes spp. was found on round areas with a diameter of 1 to 3 cm in the glandular mucosa of the stomach. Moderate amount of PCV2 viral antigen was detected almost evenly in the stomach and mostly in the macrophages. In addition, acute uraemia, revealed by an ammonia-like stink of the gastric mucosa and the presence of acute erosions on the glandular mucosa of the stomach, was observed as a consequence of PCV2-induced in... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675754 Sun, 01 Jun 2008 04:00:00 +0100 1675754 http://www.medworm.com/index.php?rid=1675753&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669249%26dopt%3DAbstract Authors: Szeredi L, Jánosi S, Tenk M Klebsiella (K.) oxytoca infection induced the abortion of a female equine fetus in the 10th month of pregnancy. Bacteria were cultured from the liver, lung and stomach content. They were labelled with an anti-Mycobacterium bovis antibody in the thymus, liver and lungs and were stained with Giemsa and Brown-Brenn staining in the thymus and lung. The diffusely consolidated lungs contained numerous grey-whitish foci 2-4 mm in diameter, which corresponded to severe pyogranulomatous pneumonia characterised by numerous intraalveolar neutrophils and macrophages and multinucleated Langhans' giant cells. K. oxytoca was located in the cytoplasm of these cells, and extracellularly in the lumen of alveoli, bronchioles and bronchi, in the capsule of thymus ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675753 Sun, 01 Jun 2008 04:00:00 +0100 1675753 http://www.medworm.com/index.php?rid=1675752&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669250%26dopt%3DAbstract Authors: Knapczyk K, Duda M, Szafranska B, Wolsza K, Panasiewicz G, Koziorowski M, Slomczynska M The sites of oestrogen action can be shown by the localisation of their receptors in the target tissues. The aim of the present study was to show the localisation of oestrogen receptors in porcine embryos and fetuses obtained on days 18, 22, 32, 40, 50, 60, 71 and 90 post coitum (p.c.). The visualisation of proteins was conducted in embryos and various fetal organs such as gonads, uterus, lung, kidney, intestine and adrenal gland. Both ERs were observed in the blastocysts on day 18 p.c. In the male, ERbeta was detected in the testis and epididymis, whereas ERalpha was present in the efferent ductules. In the female, ERbeta was detected in the ovarian stromal cells investing the oocyte nests... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675752 Sun, 01 Jun 2008 04:00:00 +0100 1675752 http://www.medworm.com/index.php?rid=1675751&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669251%26dopt%3DAbstract Authors: Filipović N, Stojević Z, Zdelar-Tuk M, Kusec V The first weeks of lactation in dairy cows are characterised by elevated bone resorption. The connection between lactation and bone metabolism is still much discussed. In this work, changes in the concentration of plasma parathyroid hormone-related peptide (PTHrP) and markers of bone metabolism were studied in Holstein cows and heifers in the dry period and early lactation to determine the role of PTHrP in the relationship between the rate of bone remodelling and the onset of lactation in dairy cows. Blood samples were taken 14 days before calving ('D-14', n = 23) and then on day 10 ('D+10', n = 21) and day 30 after calving ('D+30', n = 23). Using enzyme immunoassay (EIA), the concentrations of PTHrP, parathyroid hormo... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675751 Sun, 01 Jun 2008 04:00:00 +0100 1675751 http://www.medworm.com/index.php?rid=1675750&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669252%26dopt%3DAbstract Authors: Yan CL, Yang QE, Zhou GB, Hou YP, Zhao XM, Fan ZQ, Liu MQ, Liu L, Zhu SE The present study was designed to investigate the cryotolerance of in vitro fertilised (IVF) mouse embryos at various preimplantation developmental stages. IVF mouse embryos were vitrified by the open-pulled straw (OPS) method. After warming, embryos were morphologically evaluated and assessed by their development to blastocysts, hatched blastocysts or term. The results showed that a high proportion (93.3-100.0%) of vitrified embryos at all developmental stages were morphologically normal after recovery. The developmental rate of vitrified 1-cell embryos to blastocyst (40.0%) or hatched blastocyst (32.7%) or term (9.3%) was significantly lower than that from other stages (P < 0.05). Vitrified embryos f... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675750 Sun, 01 Jun 2008 04:00:00 +0100 1675750 http://www.medworm.com/index.php?rid=1675749&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669253%26dopt%3DAbstract In this report ten recently emerged IBD viruses (IBDV) were characterised by sequence analysis of the VP2 hypervariable region and compared to previous Slovene IBDV strains from 1995/1996 and to some representative serotype 1 IBDV strains of different pathotypes. On the basis of nucleotide and amino acid identities, phylogenetic analyses and the presence of very virulent IBDV (vvIBDV) conserved amino acid substitutions, all Slovene isolates from recent outbreaks were identified as vvIBDV. Although some unique nucleotide exchanges and amino acid substitutions have been observed, the results of this study indicated that recent vvIBDV isolates are closely related with those from outbreaks in the 1990s. However, acute IBD has not been reported in commercial flocks in Slovenia for some years. T... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675749 Sun, 01 Jun 2008 04:00:00 +0100 1675749 http://www.medworm.com/index.php?rid=1675748&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18669254%26dopt%3DAbstract In this study, a protocol for the high-level expression and purification of recombinant virion protein 1 (rVP1) was established using metal-chelate affinity chromatography under denaturing condition. When used as a coating antigen in a prototype enzyme-linked immunosorbent assay (ELISA), denatured rVP1 was recognised by ERBV1 antibody present in horse serum. This finding suggests that denatured rVP1 is a promising candidate for the development of an ELISA to be used in the routine laboratory diagnosis of ERBV1 infection in horses. PMID: 18669254 [PubMed - in process] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1675748 Sun, 01 Jun 2008 04:00:00 +0100 1675748 http://www.medworm.com/index.php?rid=1572449&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401951%26dopt%3DAbstract Authors: Illyés G, Kovács K, Kocsis B, Baintner K It is known that bacterial lipopolysaccharide (LPS) is not absorbed from the gastrointestinal tract in significant quantities. However, the question remained whether oral LPS modified the structure or function of the gut. In the present experiment Escherichia coli 083 LPS was administered to growing rats in repeated oral doses of 400 mg/kg body weight (b. w.), every 8 h. After three days of treatment, morphometric and histochemical examinations of the small intestine did not show significant differences between treated and control rats. It is concluded that repeated oral administration of high doses of E. coli 083 LPS had no demonstrable effect on intestinal structure and cell proliferation in a rat model. PMID: 18401951 [... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572449 Sat, 01 Mar 2008 05:00:00 +0100 1572449 http://www.medworm.com/index.php?rid=1572448&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401952%26dopt%3DAbstract Authors: Fekete PZ, Nagy B To study the genetic characteristics of DT104 strains of Salmonella Typhimurium and the prevalence of Salmonella Genomic Island (SGI1) in Hungary, 140 recent Salmonella strains of food and animal origin were examined. For the first time in Hungary, the SGI1 was found in 17 out of 59 S. Typhimurium isolates (all proven to be DT104 phage type). These 17 strains were then subtyped by pulsed-field gel electrophoresis (PFGE) into 6 pulsotypes which were less correlated with the geographic origin than with the animal species of origin. PMID: 18401952 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572448 Sat, 01 Mar 2008 05:00:00 +0100 1572448 http://www.medworm.com/index.php?rid=1572447&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401953%26dopt%3DAbstract This study indicates that with the reduced use of antibiotics in food animals, it is possible to decrease the rate of resistant bacteria. Although the use of avoparcin had been banned in 1998, the VRE strains disappeared only five years later. PMID: 18401953 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572447 Sat, 01 Mar 2008 05:00:00 +0100 1572447 http://www.medworm.com/index.php?rid=1572446&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401954%26dopt%3DAbstract This study investigates the effect of altering the composition (adjuvants and/or addition of formalin-inactivated P. multocida toxin, fPMT) of conventional vaccines on the serological profile and on protection against AR in swine. A significantly higher B. bronchiseptica specific antibody titre was detected for vaccines with novel immunostimulants, the best being Montanide IMS 1313 (1:630 compared to 1:274 obtained with alum). The highest B. bronchiseptica antibody titre was demonstrated for a combination of B. bronchiseptica--fPMT, while PMT antibody titre was highest for monovalent fPMT (both adjuvanted with IMS 1313). The AR-specific antibodies were transmitted from dams to their offspring in similar titres and with the same hierarchy of effectiveness. After a B. bronchiseptica--P. mult... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572446 Sat, 01 Mar 2008 05:00:00 +0100 1572446 http://www.medworm.com/index.php?rid=1572445&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401955%26dopt%3DAbstract Authors: Bersényi A, Berta E, Kádár I, Glávits R, Szilágyi M, Fekete SG To study the effects of high dietary molybdenum (Mo) content, rabbits were fed with commercial pellets and carrots containing 39 mg Mo/kg dry matter (DM) [Experiment 1] and with a commercial diet supplemented with 40 mg Mo/kg DM [Experiment 2] for 14 days. The high dietary Mo contents failed to reduce the growth performance of rabbits. Moreover, supplemental Mo given in a dose of 40 mg/kg non-significantly decreased the apparent digestibility of crude protein (CP) and crude fibre (CF) compared to the control (73.63 +/- 2.49 and 18.56 +/- 5.10 vs. 74.31 +/- 3.03 and 21.38 +/- 6.48, respectively). Molybdenum ingested with feeds was mainly excreted (57%) via the urine. The highest Mo levels we... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572445 Sat, 01 Mar 2008 05:00:00 +0100 1572445 http://www.medworm.com/index.php?rid=1572444&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401956%26dopt%3DAbstract Authors: Karcagi RG, Gaál T, Wágner L, Husvéth F In a model experiment, Holstein-Friesian dairy cows were fed on a corn-silage-based diet supplemented with 11.75 MJ NE1 per day of calcium soaps of palm oil fatty acids (CAS) or hydrogenated triglyceride (HTG) or without fat supplementation (control). All diets were fed to the cows over a period from 21 +/- 3 days (d) prior to the expected calving to d 100 +/- 5 postpartum. On d 25 (basal sample) and d 14 prepartum as well as on d 5 and 25 postpartum liver samples were collected by percutaneous biopsy. Total lipid content, fatty acid composition and glycogen of liver tissues were determined. At d 5 postpartum, both control and CAS cows had higher liver lipid (P < 0.05) and lower glycogen (P < 0.05) concentrations tha... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572444 Sat, 01 Mar 2008 05:00:00 +0100 1572444 http://www.medworm.com/index.php?rid=1572443&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401957%26dopt%3DAbstract Authors: Hassanzadeh M, Buyse J, Decuypere E Eggs from a broiler line were incubated at two different altitudes and hatched. Relative heart and lung weights, volumes of the heart, lung and thoracic cavity, incidence of right ventricular hypertrophy and ascites, and related physiological parameters were followed in the day-old chickens hatched from the above eggs. Lung and heart weights as a percentage of body weight, lung and heart volumes relative to the volume of the thoracic cavity after removing the heart and lungs were higher in chickens hatched at high altitude. Additionally, embryonic triiodothyronine (T3) and thyroxine (T4) levels relative to cardiopulmonary parameters were higher in day-old chickens that hatched at high altitude as compared with chickens hatched at low altitud... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572443 Sat, 01 Mar 2008 05:00:00 +0100 1572443 http://www.medworm.com/index.php?rid=1572442&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401958%26dopt%3DAbstract Authors: Hornok S, Edelhofer R, Joachim A, Farkas R, Berta K, Répási A, Lakatos B Blood samples were collected from 330 cats in Hungary in order to evaluate their seroconversion to Toxoplasma gondii and Neospora caninum using the indirect fluorescent antibody test (IFAT). The overall prevalence of toxoplasmosis was 47.6%, the prevalence being 22.4% among urban, 50% among suburban and 61.3% among rural animals. Significantly more cats had high IFAT titres (1:640 to 1:5120) in the countryside. Female cats were more frequently infected with T. gondii than males (53.3% vs. 39.3%), and seropositivity increased with the age of animals. The prevalence (0.6%) and titre (1:40) of antibodies to N. caninum was low. Sixty-two cats were also screened for seroconversion to feline infectiou... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572442 Sat, 01 Mar 2008 05:00:00 +0100 1572442 http://www.medworm.com/index.php?rid=1572441&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401959%26dopt%3DAbstract Authors: Szeredi L, Glávits R, Tenk M, Jánosi S The applicability of an anti-Mycobacterium bovis (BCG) antibody-based immunohistochemistry (IHC) procedure was investigated using everyday veterinary pathological samples collected from 13 different animal species. Fifty-one formalin-fixed and paraffin-embedded tissue samples were selected for this study. Forty, 4 and 7 tissue samples contained different species of bacteria, fungi and protozoa, respectively. Three serial sections were prepared in each case. Two sections were pre-treated with enzyme and heat, respectively, while the last section was not pre-treated. In seven cases the sensitivity of histochemical staining (HSM), IHC and bacteriological culture were compared. Heating of the sections in a microwave oven was the mos... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572441 Sat, 01 Mar 2008 05:00:00 +0100 1572441 http://www.medworm.com/index.php?rid=1572440&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401960%26dopt%3DAbstract Authors: Szeredi L, Szentirmai C Severe disease induced by porcine circovirus type 2 (PCV2) was observed in three pigs originating from a large herd affected by respiratory and digestive signs as well as wasting. Proliferative and necrotising pneumonia (PNP) was diagnosed in two animals, while severe acute interstitial pneumonia characterised by the presence of abundant hyaline membrane in the alveoli and fibrin in the bronchioles was found in one pig. In all cases, large amounts of PCV2 antigen were found in each tissue sample collected from the lungs and mediastinal lymph nodes. Neither porcine reproductive and respiratory syndrome virus (PRRSV) nor swine influenza virus (SIV) was detected, and no bacteria could be cultured in any of the cases. Vascular lesions, e.g. degeneration of ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572440 Sat, 01 Mar 2008 05:00:00 +0100 1572440 http://www.medworm.com/index.php?rid=1572439&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401961%26dopt%3DAbstract Authors: Deim Z, Palmai N, Cserni G Two cases of feline vaccine-associated fibrosarcoma (FVAF) are reported. The excised tumours were both characterised as well circumscribed, subcutaneous, firm and white with central necrosis. Histopathologically, they consisted of well-differentiated and variably sized and shaped anaplastic cells, characterised by marked nuclear and cellular pleomorphism including giant cells. The mitotic activity was low. Aluminium was demonstrated in the central necrosis and giant cells. Neoplastic cells were positive for vimentin and negative for desmin and cytokeratin. The presence of feline sarcoma virus and feline immunodeficiency virus could not be detected by PCR in either case. PMID: 18401961 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hunga... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572439 Sat, 01 Mar 2008 05:00:00 +0100 1572439 http://www.medworm.com/index.php?rid=1572438&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401962%26dopt%3DAbstract Authors: Guvenc T, Yarim M, Gulbahar MY, Kabak YB The cerebella of 12 dogs infected with canine distemper virus (CDV) and those of three normal dogs were examined. The avidin-biotin-peroxidase complex technique was used to detect alphaB-crystallin (alphaB-c) immunoreactivity and immunolocalisation of the CDV antigen. CDV antigens, immunopositive astrocytes, oligodendrocytes and granular neurons were seen in both the white and grey matter of the infected dogs. In the controls, alphaB-c immunopositive glial cells were seen in the white matter and around the Purkinje cells. In dogs with distemper, alphaB-c immunoreactivity was not observed in some of the glial cells around the Purkinje cells. A significant negative correlation of P < 0.01 level was found between areas of severe demyeli... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572438 Sat, 01 Mar 2008 05:00:00 +0100 1572438 http://www.medworm.com/index.php?rid=1572437&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401963%26dopt%3DAbstract Authors: López-Bayghen C, Zozaya H, Ocampo L, Brumbaugh GW, Sumano H The efficacy of melengestrol acetate (MGA) to shorten the vernal transition of mares by synchronising and accelerating the first ovulation of the year after 60 days of phototherapy was determined by ultrasonographic monitoring. Sixteen mares in late transition were fed two doses of MGA (150 mg/mare/day and 100 mg/mare/day, respectively) for 10 days. A luteolytic dose of prostaglandin was administered to each mare one day after the end of MGA treatment. The presence and duration of oestrus, follicular growth, uterine oedema and presence of ovulation were monitored by ultrasonography and the cervical tone was evaluated by rectal palpation. Ovulation was detected in 87.5% of the mares treated with 150 mg MGA/mare/da... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572437 Sat, 01 Mar 2008 05:00:00 +0100 1572437 http://www.medworm.com/index.php?rid=1572436&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401964%26dopt%3DAbstract Authors: Jankovicová J, Simon M, Antalíková J, Horovská L Artificial insemination with frozen-thawed spermatozoa is commonly used in cattle breeding. A simple and fast procedure is needed for routine evaluation of the acrosomal status of frozen-thawed bovine sperm. Therefore, the purpose of this study was to test two staining procedures used to determine the viability and integrity of acrosome of frozen-thawed bovine spermatozoa. Double staining and Hoechst/FITC-Pisum sativum agglutinin (FITC-PSA) labelling were tested for evaluating the viability and acrosome reaction induced by calcium ionophore of bull spermatozoa. In our experiments no significant differences were detected in the frequency of acrosome-reacted sperm either by double staining (37.98%) or by FITC-P... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572436 Sat, 01 Mar 2008 05:00:00 +0100 1572436 http://www.medworm.com/index.php?rid=1572435&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401965%26dopt%3DAbstract Authors: Cságola A, Kiss I, Tuboly T Porcine circovirus type 1 (PCV1) is considered to be a non-pathogenic virus detected in cell cultures, vaccines or products used for cell culture preparations, all of them of porcine origin. Serological evidence and genetic studies suggested that PCV1 was widespread in domestic pigs. The presence of PCV1 in wild boars in Germany was also described using serological methods. This paper reports the first detection of PCV1 in Hungarian wild boars. Samples were collected at slaughterhouses and processed for polymerase chain reactions. The complete genome of PCV1 detected in the samples was determined and compared with the available PCV1 sequences of the GenBank database. The genomes formed two distinct clusters with minimum differences, where the H... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572435 Sat, 01 Mar 2008 05:00:00 +0100 1572435 http://www.medworm.com/index.php?rid=1572461&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277701%26dopt%3DAbstract This article describes the scanning electron microscopic (SEM) features of the ovine interdigital sinus. The lumen was filled with a dense secretory material and quite a number of hairs embedded in the luminal content. For SEM purposes, the sinus was divided into three parts: base, body and neck. At the cut surface, the wall exhibited significant folds which were almost absent in the base, the very short blind end of the sinus. The wall had three layers: epidermis, dermis and fibrous capsule. Stratified epithelium with a prominent keratin layer faced the lumen. The inner surface was similar to the skin surface; however, it was coarser due to folds. The fibrous capsule was composed mainly of dense connective tissue, constituting the outermost layer of the wall. The dermis contained common s... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572461 Sat, 01 Dec 2007 05:00:00 +0100 1572461 http://www.medworm.com/index.php?rid=1572460&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277702%26dopt%3DAbstract Authors: Varga Z, Sellyei B, Magyar T A total of 146 Pasteurella multocida strains isolated from swine in Hungary in the last 20 years were examined. Biochemical characterisation and PCR-based techniques were used to determine species, subspecies, biovar, capsule type and presence of the toxA gene. Eighty-seven percent of the isolates belonged to P. multocida ssp. multocida, and 98% of these had biovar 3 or were trehalose- or lactose-fermenting or ornithine decarboxylase negative variants of that. Ten percent of the strains were P. multocida ssp. septica, and within this group 80% of the strains showed sorbitol-negative biovars (5, 6 and 7). The rest of the strains (20%) were lactose positive. Only 3% of the porcine isolates were P. multocida ssp. gallicida and 3 out of the 4 strains b... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572460 Sat, 01 Dec 2007 05:00:00 +0100 1572460 http://www.medworm.com/index.php?rid=1572459&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277703%26dopt%3DAbstract The objective of the study was to examine the expression of the genes coding for proopiomelanocortin (POMC), proenkephalin (PENK) and prodynorphin (PDYN) in porcine luteal cells isolated from corpora lutea (CL) collected on days 3-6, 8-10 and 13-16 of the oestrous cycle. Total RNA was purified from non-incubated cells and from cells incubated for 48 h in the absence or presence of luteinising hormone (LH). The semi-quantitative RT-PCR technique, involving coamplification of the target and control cDNA (beta-actin or 18S rRNA), was used to examine gene expression. It was found that the genes coding for opioid precursors are expressed in both non-incubated and incubated porcine luteal cells representing the early, mid- and late luteal phase. In non-incubated cells, only POMC mRNA content cha... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572459 Sat, 01 Dec 2007 05:00:00 +0100 1572459 http://www.medworm.com/index.php?rid=1572458&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277704%26dopt%3DAbstract Authors: Szabó A, Milisits G Commercial broilers were raised in a 5-week period and a detailed clinicochemical follow-up was carried out, to characterise a flock selected for one-sided muscle mass production. Blood samples were drawn at the ages of 1 day, 1, 2, 3, 4 and 5 weeks, and plasma enzyme activities, metabolite and ion concentrations were determined. Early increases were found for all plasma nitrogenous compounds (total protein, albumin, creatinine and urate). Triglyceride showed a posthatch peak with a significant effect of age. Plasma total cholesterol was characterised by a marked post-hatch concentration peak, while during the first week its concentration decreased markedly. Plasma AST showed an increase during the rearing, while a one-magnitude increment was found for... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572458 Sat, 01 Dec 2007 05:00:00 +0100 1572458 http://www.medworm.com/index.php?rid=1572457&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277705%26dopt%3DAbstract Authors: Balogh K, Hausenblasz J, Weber M, Erdélyi M, Fodor J, Mézes M The effect of feeding ochratoxin A (OTA) contaminated diet (379.6 and 338.1 microg/kg in starter and grower diets) on production traits, lipid peroxidation and some parameters of the glutathione redox system were investigated in weaned piglets over a seven-week period. Feed intake and feed conversion ratio (FCR) did not differ significantly, but in the first phase (0-28 days) the daily weight gain was significantly lower in the piglets fed the OTA-contaminated diet. Lipid peroxidation, as measured by the amount of malondialdehyde, glutathione content and glutathione peroxidase activity, did not change significantly in the blood plasma and red blood cell haemolysate in the OTA-loaded group, while malondiald... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572457 Sat, 01 Dec 2007 05:00:00 +0100 1572457 http://www.medworm.com/index.php?rid=1572456&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277706%26dopt%3DAbstract The objective of this study was to determine the presence of Malassezia spp. in the external ear canal of cats with and without otitis. Forty-five animals were studied, 20 with and 25 without otitis externa (OE). Cerumen or secretion from external ear canal samples was cultured on modified Mycosel agar and sterile olive oil was added to the surface of the medium before specimen seeding. The isolates were analysed for macro- and micromorphology and identified by catalase tests and on the basis of growth on Tween 20, 40, 60 and 80. Malassezia spp. were isolated from 15 out of 20 (75%) animals with otitis and from 7 out of 25 (28%) cats without OE; the difference between the two groups was statistically significant (P < or = 0.05). Malassezia pachydermatis and M. sympodialis were isolated ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572456 Sat, 01 Dec 2007 05:00:00 +0100 1572456 http://www.medworm.com/index.php?rid=1572455&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277707%26dopt%3DAbstract Authors: Senkoylu N, Samli HE, Kanter M, Agma A Day-old male chicks were used to test the effects of a combination of formic and propionic acids (CFP) added to wheat- and barley-based diets on the performance and gut histomorphology of broilers. The CFP, containing formic and propionic acids based on an inorganic phyllo-silicate carrier, was added to starter and grower broiler diets of standard (NRC, 1994) or lower nutrient density (LND) using different levels of wheat and barley. Body weight gain (BWG) and feed conversion ratio (FCR) of broiler chicks were significantly (P < 0.001) improved by CFP supplementation at 21 days of age regardless of nutrient density (ND). However, at 35 days of age CFP addition significantly (P < 0.001) increased BWG and FCR. Proventriculus weight wa... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572455 Sat, 01 Dec 2007 05:00:00 +0100 1572455 http://www.medworm.com/index.php?rid=1572454&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277708%26dopt%3DAbstract Authors: Hornok S, Edelhofer R, Földvári G, Joachim A, Farkas R In order to evaluate the seroconversion of horses to Babesia caballi and B. canis in Hungary, blood samples were collected from 371 animals on 23 different locations of the country. The presence of antibodies to B. caballi was screened with a competitive ELISA. All 29 positive samples came from one region (the Hortobágy). The prevalence of infection did not show correlation with sexes, and reached 100% in the age group of 2-5 years. Babesia canis-specific antibodies were demonstrated by IFAT in 6.74% of animals kept in 7 regions. The titres were low or medium level (1:40 to 1:160), indicating that the horses had previously been exposed to this piroplasm, but their infection must have been limited. The highes... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572454 Sat, 01 Dec 2007 05:00:00 +0100 1572454 http://www.medworm.com/index.php?rid=1572453&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277709%26dopt%3DAbstract Authors: Dayoub A, Molnár K, Salman H, Al-Samman A, Székely C During a survey on Myxobolus infection of pond-cultured common carp in Syria three Myxobolus spp. were found. Myxobolus dispar infected the gill arteries, forming large elongated plasmodia in the gill filaments. The plasmodia of M basilamellaris were located in the gill arches at the base of the filaments. Elongated filiform plasmodia of M. encephalicus were found in the blood vessels of the brain. Despite the common occurrence of the above parasites, no disease symptoms were observed in the infected fish specimens. This is the first report on myxosporean infection of fish from Syrian waters. PMID: 18277709 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572453 Sat, 01 Dec 2007 05:00:00 +0100 1572453 http://www.medworm.com/index.php?rid=1572452&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277710%26dopt%3DAbstract Authors: Máthé A, Dobos-Kovács M, Vörös K Histological and electron microscopic examinations of the kidneys of 8 dogs suffering from fatal, naturally acquired Babesia canis infection and nephropathy are presented. Seven animals were treated with imidocarb dipropionate on average 4.5 days prior to death. Severe anaemia was present only in 2 cases. Degenerative histological changes observed mostly in the proximal convoluted tubules included vacuolar-hydropic degeneration, necrosis and detachment of renal tubular epithelial (RTE) cells from the basement membrane. Necrotic debris occasionally formed acidophilic casts within the tubules. In some cases, necrosis of the whole tubule was observed. Haemoglobin casts in the tubules and haemoglobin droplets in RTE cells s... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572452 Sat, 01 Dec 2007 05:00:00 +0100 1572452 http://www.medworm.com/index.php?rid=1572451&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277711%26dopt%3DAbstract Authors: Szeredi L, Pospischil A, Dencsö L, Mathis A, Dobos-Kovács M A Lippizan mare aborted a male fetus a few days before the expected foaling date without showing any clinical sings. Focal lympho-histiocytic hepatitis in the foal and multiplex focal lympho-histiocytic villitis accompanied by villus necroses and marked hypertrophy of chorionic epithelial cells in the arcades were observed. Elongated nucleated organisms were seen in groups in vacuoles or solitarily located in the cytoplasm of the chorionic epithelial cells. The organisms were in large numbers and often extracellularly in areas of villitis and villus necroses. They were Gram-positive, stained with haematoxylin and eosin (HE), periodic acid-Schiff (PAS) and Giemsa, weakly with Warthin-Starry silver stain but n... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572451 Sat, 01 Dec 2007 05:00:00 +0100 1572451 http://www.medworm.com/index.php?rid=1572450&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277712%26dopt%3DAbstract In conclusion, our findings emphasise the diagnostic and prognostic value of skin biopsy analysis for acute GVHD after Itx. PMID: 18277712 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572450 Sat, 01 Dec 2007 05:00:00 +0100 1572450 http://www.medworm.com/index.php?rid=1572475&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867454%26dopt%3DAbstract Authors: Bakos Z, Vörös K Since percussion started to be a method forgotten and not trusted by many practitioners, the aim of the study was to demonstrate whether this procedure could deliver exact data if done accurately and by a practised examiner. The trial presented here included 31 healthy warm-blooded horses. Percussion of the area of cardiac dullness and then echocardiographic imaging of the same region were performed. To obtain the relevant measurement points, the 4th and 5th intercostal spaces (ICS) were used on the left and the 4th ICS on the right side. On the left side, the dorsal border of cardiac dullness was determined in the 4th ICS (1st point). Then the ventral border of the cardiac dullness was determined at the same place (2nd point). This was followed by t... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572475 Sat, 01 Sep 2007 04:00:00 +0100 1572475 http://www.medworm.com/index.php?rid=1572474&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867455%26dopt%3DAbstract Authors: Gaál T, Halmay D, Kocsis R, Abonyi-Tóth Z The effect of two nonsteroidal anti-inflammatory drugs (carprofen and ketoprofen) on platelet adhesion and aggregation functions was evaluated by the PFA-100 analyser (Dade-Behring, CA, U.S.A.) using its collagen-adenosine diphosphate (ADP) and collagen-epinephrine (EPI) cartridges. The function of platelets was evaluated in 55 healthy dogs, in 7 dogs treated with ketoprofen and in 31 dogs treated with carprofen in a therapeutic dose for minimum 5 days. The therapeutic doses of carprofen had no effect on the closure time of PFA-100 (which is the marker of platelet function) but ketoprofen caused a significant increase when using collagen-EPI stimulation The closure times for both the healthy (control) and the treated dogs usi... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572474 Sat, 01 Sep 2007 04:00:00 +0100 1572474 http://www.medworm.com/index.php?rid=1572473&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867456%26dopt%3DAbstract Authors: Parra MD, Cerón JJ C-reactive protein (CRP) and haptoglobin (Hp) are well-known acute phase proteins in the dog. Currently, a commercial ELISA and a colorimetric assay are the methods of choice for measuring CRP and Hp, respectively; however, these assays showed interference when using haemolysed, lipaemic or hyperbilirubinaemic samples. Recently, time-resolved immunofluorometric assays (TR-IFMAs) have been developed for measuring canine CRP and Hp. The aim of the present study was to evaluate the effect of increasing concentrations of haemoglobin, lipids and bilirubin in CRP and Hp serum measurements using these new fluoroimmunoassays. Haemolysis was produced by freezing blood cells at -20 degrees C. The haemolysate was added to pooled sera at final concentrations of 0, ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572473 Sat, 01 Sep 2007 04:00:00 +0100 1572473 http://www.medworm.com/index.php?rid=1572472&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867457%26dopt%3DAbstract Authors: Khol JL, Licka TF Hip luxations have been reported in many ruminants, and several treatments have been described for them. However, successful closed reduction of hip joint luxation has not been reported in goats to date. This case report describes the closed reduction of coxofemoral luxation in a three-month-old male Saanen dairy goat. The goat showed non-weightbearing lameness and abnormal mobility of the proximal right hindlimb. Luxation of the right coxofemoral joint in craniodorsal direction was diagnosed by radiography. The femoral head was repositioned under sedation, and physiotherapy including non-weightbearing hindlimb movement and supported walking exercise was performed. Fifteen days after closed reduction the animal was discharged from the clinic. At that time the... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572472 Sat, 01 Sep 2007 04:00:00 +0100 1572472 http://www.medworm.com/index.php?rid=1572471&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867458%26dopt%3DAbstract Authors: Bugno M, Słota E A 5-year-old infertile Hutsul mare was subjected to cytogenetic analysis. Fluorescence in situ hybridisation (FISH) using the equine Xp and Xq chromosome painting probes was carried out on chromosome preparations obtained after blood lymphocyte culture. These probes were generated by chromosome microdissection and a large number of spreads was analysed (525). The karyotype formula of the analysed mare was 64,XX/65,XX+Xp with the ratio of the two lines being 99.4 and 0.6, respectively. The goal of the study was to apply chromosome microdissection and the FISH technique for cytogenetic diagnostics. PMID: 17867458 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572471 Sat, 01 Sep 2007 04:00:00 +0100 1572471 http://www.medworm.com/index.php?rid=1572470&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867459%26dopt%3DAbstract Authors: Ivanics E, Dobos-Kovács M, Glávits R, Kaszanyitzky E, Nemes C, Szeredi L, Beregszászi A, Dencso L Ten one-day-old goslings were inoculated orally with a Brachyspira alvinipulli strain isolated from the large intestine of geese that had died of intestinal spirochaetosis (Group A), 10 day-old goslings were inoculated orally with a B. hyodysenteriae strain (Group B), and a third group of 10 goslings (Group C) served as uninfected control. The goslings were observed daily for clinical signs. They were sacrificed on days 7, 14, 21 and 35 days postinfection (PI), and necropsied. Segments of the large intestine were subjected to histopathological, immunohistochemical, electron microscopic (TEM, SEM) and microbiological examinations. Mortality did not occur during the e... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572470 Sat, 01 Sep 2007 04:00:00 +0100 1572470 http://www.medworm.com/index.php?rid=1572469&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867460%26dopt%3DAbstract Authors: Gál J, Jakab C, Balogh B, Tóth T, Farkas B The occurrence of a periosteal chondroma (juxtacortical chondroma) in an adult male Uromastyx maliensis is documented for the first time. The chondroma developed near the right shoulder joint from the periosteal membrane, causing partial atrophy in the surrounding skeletal muscles. In the chondroma tissues widespread central necrosis and secondary calcium salt deposition were observed. Monomorphic chondrocytes were irregularly spread in the chondromucin matrix. The lizard had locomotor problems due to irritation of the periosteum and reduced movement of the bones constituting the shoulder joint caused by the chondroma. PMID: 17867460 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572469 Sat, 01 Sep 2007 04:00:00 +0100 1572469 http://www.medworm.com/index.php?rid=1572468&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867461%26dopt%3DAbstract Authors: Zsarnovszky A, Földvári EG, Rónai Z, Bartha T, Frenyó LV Environmental and plant oestrogens have been identified as compounds that when ingested, disrupt the physiological pathways of endogenous oestrogen actions and thus, act as agonists or antagonists of oestrogen. Although the risks of exposure to exogenous oestrogens (ExEs) are subject to scientific debate, the question of how ExE exposure affects the central nervous system remains to be answered. We attempt to summarise the mechanisms of oestrogenic effects in the central nervous tissue with the purpose to highlight the avenues potentially used by ExEs. The genomic and rapid, non-genomic cellular pathways activated by oestrogen are listed and discussed together with the best known interneuronal mechani... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572468 Sat, 01 Sep 2007 04:00:00 +0100 1572468 http://www.medworm.com/index.php?rid=1572467&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867462%26dopt%3DAbstract Authors: Kovács BM, Toussaint MJ, Gruys E, Fábián IB, Szilágyi L, Janan J, Rudas P Serum amyloid A (SAA) is of interest as the circulating precursor of amyloid A protein, the fibrillar component of AA (secondary) amyloid deposits, and also as an extremely sensitive and rapid major acute phase protein. Serum concentrations of acute phase proteins (APPs) provide valuable information about the diagnosis and prognosis of various diseases, and thus the relevance of APPs for monitoring the health status of domestic animals is widely accepted. More importantly, the measurement of SAA concentration assists in assessing the prognosis in secondary amyloidosis, which is a common disease of geese, affecting an increasing number of animals. In the present study we introduce a hi... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572467 Sat, 01 Sep 2007 04:00:00 +0100 1572467 http://www.medworm.com/index.php?rid=1572466&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867463%26dopt%3DAbstract Authors: Kamińska B, Opalka M, Dusza L The effect of plant-derived, nonsteroidal estrogens (genistein, daidzein and biochanin A) on cortisol and androstenedione secretion by porcine adrenocortical cells was examined. Adrenals were harvested from locally slaughtered mature gilts on days 5-9 of the oestrous cycle. Adrenocortical cells were incubated with or without genistein, daidzein, biochanin A (0.5, 5, 10 or 50 microM), oestradiol (10, 50, 100 or 500 pg/mL) and ACTH (5 nM--positive control). All incubations were performed for 8 h (95% air and 5% CO2, 37 degrees C). ACTH increased cortisol and androstenedione secretion. Genistein, daidzein and biochanin A suppressed cortisol output, whereas androstenedione secretion was enhanced by these phytoestrogens. In contrast, oestradiol d... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572466 Sat, 01 Sep 2007 04:00:00 +0100 1572466 http://www.medworm.com/index.php?rid=1572465&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867464%26dopt%3DAbstract Authors: Meng Q, Li X, Wu T, Dinnyés A, Zhu S The present study was designed to investigate fertilisation of open pulled straw (OPS) vitrified mouse oocytes drilled with piezo-micromanipulation method and their subsequent in vitro and in vivo developmental capacity. Ovulated mouse oocytes were vitrified using the OPS method. After warming, the zona pellucida of a group of vitrified-warmed oocytes was drilled by piezo-micromanipulation. Groups of (a) vitrified, (b) vitrified/drilled and (c) fresh control oocytes were fertilised in vitro. The fertilisation rate of vitrified-warmed oocytes was significantly lower than that of fresh oocytes (45.0 +/- 12.6% vs. 85.2 +/- 6.8%, P < 0.05), and was significantly improved by zona-drilling (85.4 +/- 7.3%). However, blastocyst formation ra... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572465 Sat, 01 Sep 2007 04:00:00 +0100 1572465 http://www.medworm.com/index.php?rid=1572464&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867465%26dopt%3DAbstract This study investigated the effects of ovariohysterectomy performed at 10 weeks of age on body weight, behaviour, bone length, serum calcium and phosphorus concentrations and gonadal hormone (progesterone and oestradiol) levels until 24 weeks of age in 31 clinically healthy mixed-breed female puppies. The littermates were randomly assigned to three groups. Group I (n=11) was completely ovariohysterectomised, Group II (n=10) was incompletely ovariohysterectomised with one ovary left intact, and Group III (n=10) was sham operated. No significant differences were found between the groups for any of the parameters evaluated. It was determined that, except for two measurements of two animals in Group II at 20 and 24 weeks of age, all animals had undetectable concentrations of oestradiol 17-beta... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572464 Sat, 01 Sep 2007 04:00:00 +0100 1572464 http://www.medworm.com/index.php?rid=1572463&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867466%26dopt%3DAbstract This report describes the first case of postweaning multisystemic wasting syndrome (PMWS) in wild boar in Croatia. During the winter season of 2004, eight wild young piglets (of approximately 2 to 5 months of age) were found dead in a fenced hunting area. Polymerase chain reaction (PCR) was carried out on mesenteric lymph nodes and all animals yielded positive results. In one of these animals diagnosis of PMWS was established based on the three key diagnostic criteria including the clinical manifestation, moderate lymphoid lesions consisting of lymphocyte depletion and granulomatous inflammation, and detection of the presence of PCV2 genome within the lymphoid lesions by in situ hybridisation (ISH). Three additional wild piglets had also mild PMWS-like lesions and a low amount of PCV2 was ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572463 Sat, 01 Sep 2007 04:00:00 +0100 1572463 http://www.medworm.com/index.php?rid=1572462&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17867467%26dopt%3DAbstract In this study, instead of using whole virus, recombinant protein of hexahistidine tag (His 6 tag) and VPX protein of IBDV expressed in E. coli was used as an alternative antigen to coat the ELISA plates. There was a good correlation coefficient (R2 = 0.972) between the results of the ELISA using plates coated with monoclonal antibody against His 6 tag and those of the commercial IBDV ELISA kit. Hence, His 6 tag and VPX recombinant protein expressed in E. coli has the potential for the development of ELISA for the measurement of IBDV-specific antibody. PMID: 17867467 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572462 Sat, 01 Sep 2007 04:00:00 +0100 1572462 http://www.medworm.com/index.php?rid=1572488&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555280%26dopt%3DAbstract Authors: Mattar MA, Cortiñas TI, Stefanini AM The anaerobic bacillus Clostridium chauvoei is the causative agent of blackleg, a lethal disease that has an important impact on the sheep and cattle industry worldwide. Immunity to C. chauvoei is considered to be mainly anticellular, and for this reason there is scarce information about the immunogenicity of extracellular proteins. In this work variations in protein profiles, immune response by ELISA and protective capacity of culture supernatants of three C. chauvoei strains, collected at different growth phases, are reported. Sera raised against extracellular antigens also recognised cellular antigens of the same molecular masses. Partially purified cell-free supernatants and those concentrated 10 times by ultrafiltration (C-CFS), o... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572488 Fri, 01 Jun 2007 04:00:00 +0100 1572488 http://www.medworm.com/index.php?rid=1572487&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555281%26dopt%3DAbstract Authors: Gómez-Ochoa P, Miana-Mena FJ, Muñoz MJ, Gascón M, Castillo JA, Cativiela E, Gómez F Pluripotent stem cells (PSCs), already described in human beings, are fibroblast-like cells that exhibit a CD34 marker specific for haematopoietic stem cells. In this work we have demonstrated the presence of PSCs in the peripheral blood of pigs, a species frequently used in transplantation studies as an animal model for human diseases. Differentiation into haematopoietic colonies (granulomacrophagic colonies, erythroid colonies and mixed colonies) has been carried out with the peripheral blood of adult and newborn pigs, using solely human commercial media. Peripheral blood mononuclear cells (PBMNCs) were cultured in semisolid methylcellulose based media enriched with recomb... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572487 Fri, 01 Jun 2007 04:00:00 +0100 1572487 http://www.medworm.com/index.php?rid=1572486&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555282%26dopt%3DAbstract Authors: Milinković-Tur S, Stojević Z, Pirsljin J, Zdelar-Tuk M, Poljicak-Milas N, Ljubić BB, Gradinski-Vrbanac B The effect of fasting and refeeding on total antioxidant status (TAS), glutathione peroxidase (GSH-Px) activity and concentration of some non-enzymatic antioxidant compounds was studied in cockerels and pullets. Blood was collected before and after 48-h fasting and 24 h after refeeding. In cockerels, fasting resulted in a significant decrease of TAS and uric acid concentration. After refeeding, the concentration of TAS remained significantly lower as compared to the control level. At the same time, blood plasma level of total lipids increased in comparison to the control and post-fasting values. In pullets, fasting resulted in a significant decrease of who... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572486 Fri, 01 Jun 2007 04:00:00 +0100 1572486 http://www.medworm.com/index.php?rid=1572485&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555283%26dopt%3DAbstract Authors: Ceylan C, Aydilek N, Ipek H It is not clear whether the anaesthetic agents tiletamine and zolazepam have antioxidant or pro-oxidant effects. Therefore, this study was carried out to investigate the effects of tiletamine-zolazepam anaesthesia on oxidant/antioxidant status in blood plasma and on haematological parameters in 10 healthy Awassi ewes. The tiletamine-zolazepam combination was administrated in a dose of 7.5 mg/kg intramuscularly. The animals were spontaneously breathing air during the procedure. Blood samples were collected by jugular venipuncture before induction and at 30, 60, 120 min, 24 h and 3 days after anaesthesia. Malondialdehyde concentration, an index of lipid peroxidation, was higher at 30, 60, 120 min and 24 h (P < 0.05) than the baseline value in the p... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572485 Fri, 01 Jun 2007 04:00:00 +0100 1572485 http://www.medworm.com/index.php?rid=1572484&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555284%26dopt%3DAbstract In conclusion, the MPP needs fewer EIXXR than the SPM and complies with the new recommendations in X-ray protection, which introduce the ALARA (as-low-as-reasonably-achievable) idea. PMID: 17555284 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572484 Fri, 01 Jun 2007 04:00:00 +0100 1572484 http://www.medworm.com/index.php?rid=1572483&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555285%26dopt%3DAbstract Authors: Bugno M, Słota E, Pieńkowska-Schelling A, Schelling C An infertile mare with hypoplastic ovaries was subjected to cytogenetic analysis. Fluorescence in situ hybridisation (FISH) using the equine X whole chromosome painting probe (WCPP) was carried out on a chromosome preparation obtained from blood lymphocyte culture. The number of analysed spreads was high (235) and in the X chromosome aneuploidy in mosaic form was diagnosed. The karyotype formula was 63,X / 64,XX / 65,XXX. The ratio of the three lines was 15%, 82% and 3%, respectively. The application of the FISH technique with WCPP is discussed. PMID: 17555285 [PubMed - indexed for MEDLINE] (Source: Acta Veterinaria Hungarica) Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572483 Fri, 01 Jun 2007 04:00:00 +0100 1572483 http://www.medworm.com/index.php?rid=1572482&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555286%26dopt%3DAbstract Authors: Bistyák A, Kecskeméti S, Glávits R, Tischler I, Nagy ST, Kardos G, Kiss I An epizootic of Pacheco's disease is reported from a zoo bird population. The infection was introduced by wild-captured Patagonian conures (Cyanoliseus patagonus) despite 61 days of quarantine. The disease affected several parrot species and, interestingly, three out of seven bearded barbets (Lybius dubius). The mortality rate was 30.93%. Autopsy revealed abdominal hyperaemia with liver haemorrhages and, in less rapid cases, yellowish discoloration and fragility of the liver. Death was caused by the collapse of circulation. Histopathology demonstrated liver cell necrosis, disintegration of the lobular structure, and a few intranuclear inclusion bodies. Icosahedral virions were detected by ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572482 Fri, 01 Jun 2007 04:00:00 +0100 1572482 http://www.medworm.com/index.php?rid=1572481&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555287%26dopt%3DAbstract Authors: Biksi I, Lorincz M, Molnár B, Kecskés T, Takács N, Mirt D, Cizek A, Pejsak Z, Martineau GP, Sevin JL, Szenci O The aim of this study was to obtain prevalence estimates about the most important enteropathogenic bacteria: Lawsonia intracellularis, Brachyspira hyodysenteriae, Brachyspira pilosicoli, Salmonella enterica and Clostridium perfringens A and C in Hungarian farrow-to-finish pig herds. A total of 31 herds were selected, from where six pooled faecal samples, each containing three individual rectal faecal samples were collected from fattening pigs of 5-6 months of age. All 186 samples were examined by polymerase chain reaction (PCR) for the presence of the pathogens mentioned above. Lawsonia intracellularis was found in 29 herds (93.55%) and in 108 samples (... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572481 Fri, 01 Jun 2007 04:00:00 +0100 1572481 http://www.medworm.com/index.php?rid=1572480&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555288%26dopt%3DAbstract Authors: Fekete SG, Brown DL Nutrigenomics examines nutrient-gene interactions on a genome-wide scale. Increased dietary fat or higher non-esterified fatty acids (NEFA) from starvation-induced mobilisation may enhance hepatic oxidation and decrease esterification of fatty acids by reducing the expression of the fatty acid synthase gene. The key factors are the peroxisome proliferator-activated receptors (PPARs). Dietary carbohydrates--both independently and through insulin effect--influence the transcription of the fatty acid synthase gene. Oleic acid or n-3 fatty acids downregulate the expression of leptin, fatty acid synthase and lipoprotein lipase in retroperitoneal adipose tissue. Protein-rich diets entail a shortage of mRNA necessary for expression of the fatty acid synthase gene ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572480 Fri, 01 Jun 2007 04:00:00 +0100 1572480 http://www.medworm.com/index.php?rid=1572479&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555289%26dopt%3DAbstract The objective of our study was to determine the prevalence of gastric ulceration in a mixed population of horses by postmortem examination and to evaluate a possible association between equine gastric ulcer syndrome (EGUS) and sex or age of the examined horses, to evaluate the localisation of lesions in the proximal part of the gastrointestinal tract and to determine the occurrence of gastric parasites. Postmortem examinations were performed on 71 horses over a period of 24 months. Gastric ulcers were found in 52 horses (73.2%). There was no significant association between age or sex and occurrence of gastric ulcers. In all horses the squamous mucosa lesions were localised near the margo plicatus (100% of the cases), whereas in 23 horses the lesions were near the margo plicatus and lesser ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572479 Fri, 01 Jun 2007 04:00:00 +0100 1572479 http://www.medworm.com/index.php?rid=1572478&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555290%26dopt%3DAbstract Authors: Skipor J, Kowalik A, Stefańczyk-Krzymowska S The present study examines the direct effect of luteinising hormone (LH) on the reactivity of the porcine uterine artery to norepinephrine (NE). Three-mm-long arterial segments collected during the luteal phase of the oestrous cycle were mounted in an organ bath for isometric tension recording. After 30 min of equilibration in optimal passive tone, one part of the vessels was treated with 10 ng/ml of LH in PBS (experimental), while a second part of the arterial segments was treated with 10 ng/ml of bovine serum albumin (BSA) in PBS (control). After 30 min of equilibration, NE was given to each organ bath in a cumulative concentration manner, ranging between 1 x 10(-8) mol/l to 3 x 10(-4) mol/l. NE caused a dose-dependent contr... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572478 Fri, 01 Jun 2007 04:00:00 +0100 1572478 http://www.medworm.com/index.php?rid=1572477&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555291%26dopt%3DAbstract Authors: Simões J, Mascarenhas R The origin and evolution of preovulatory follicles (POF) in 9 hyperstimulated (polyovulatory) Serrana goats were characterised. After oestrus synchronisation and detection, transrectal ovarian ultrasonography was performed daily during two complete oestrous cycles. Blood samples were taken every 4 h during 24 h after oestrus detection for preovulatory LH peak and twice a week for plasma progesterone determinations. The interovulatory interval of 14 oestrous cycles with double ovulations was 21.1 +/- 0.3 days. The onset of ovulatory follicular wave occurred 4 days (-3.9 +/- 0.3 days, n = 14) prior to the ovulation day (day 0) with a POF size of 6.9 +/- 0.2 mm (n = 28). In goats with ovulations in both ovaries (78.6%), the emergence of the first POF ... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572477 Fri, 01 Jun 2007 04:00:00 +0100 1572477 http://www.medworm.com/index.php?rid=1572476&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17555292%26dopt%3DAbstract Authors: Sámi L, Ursu K, McKillen J, Kecskeméti S, Belák S, Kiss I Specific oligonucleotide primers were selected and combined in a multiplex arrangement, in order to detect simultaneously three economically important porcine viruses by polymerase chain reaction (PCR). The pathogen panel was comprised of viruses that cause reproductive failure in infected herds: Aujeszky's disease virus (ADV), porcine parvovirus (PPV) and porcine respiratory and reproductive syndrome virus (PRRSV). In order to reduce the time required for the detection of the pathogens, the assay was optimised to a RapidCycler PCR instrument. The multiplex PCR assay was shown to be specific, sensitive and rapid, because the results were read in less than 60 min after sample preparation. Due to its speed,... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572476 Fri, 01 Jun 2007 04:00:00 +0100 1572476 http://www.medworm.com/index.php?rid=1572502&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17385551%26dopt%3DAbstract Authors: Cvetnić Z, Spicić S, Benic M, Katalinić-Janković V, Pate M, Krt B, Ocepek M During a five-year period (2000 to 2004) 74,342 pigs were tested by the intradermal tuberculin test in Croatia. Of them, 248 (0.33%) pigs were positive and 91 (0.12%) were found to be suspicious in 7 out of the 13 farms included in the study. Gross pathological changes characteristic of tuberculosis were observed in tuberculin-positive and/or suspicious swine. Mycobacterium was isolated from the lymph nodes of 183 out of 234 swine (78.2%). For better epidemiological understanding, isolates were typed by conventional methods, PCR and hybridisation. The results show that most of the isolates belonged to the Mycobacterium avium complex (175 isolates, 95.7%). Other isolates belonged... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572502 Thu, 01 Mar 2007 05:00:00 +0100 1572502 http://www.medworm.com/index.php?rid=1572501&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17385552%26dopt%3DAbstract Authors: Lacombe-Antoneli A, Píriz S, Vadillo S The in vitro antimicrobial susceptibility of Gram-negative anaerobic bacilli commonly isolated from footrot in goats was studied. A total of 97 isolates belonging to the genera Dichelobacter, Fusobacterium, Prevotella, Porphyromonas and Bacteroides, obtained from clinical cases of footrot in south-western Spain between March 2000 and May 2001, were tested against 25 antimicrobial agents comprising beta-lactams, aminoglycosides, macrolides, chloramphenicol, quinolones, lincosamides, sulphonamides and tetracyclines in order to optimise antibiotic treatment of this disease in goats. beta-lactams, tetracyclines and metronidazole displayed the highest in vitro efficacy against the species involved in the pathogenesis of footrot. PMID:... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572501 Thu, 01 Mar 2007 05:00:00 +0100 1572501 http://www.medworm.com/index.php?rid=1572500&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17385553%26dopt%3DAbstract Authors: Weber M, Stiller S, Balogh K, Wágner L, Erdélyi M, Mézes M The purpose of the present study was to investigate the effect of experimental T-2 toxin load (2.35 mg/kg of feed) and vitamin E supply in the drinking water (10.5 mg/bird/day) on vitamin E levels of the blood plasma and liver in broiler chickens in a 14-day experiment. It was found that T-2 toxin load did not influence vitamin E content of the blood plasma except at day 3 after the toxin load when a moderate increase was detected in plasma vitamin E. No significant changes were found in vitamin E content of the liver. The simultaneous use of high-dose vitamin E supplementation and T-2 toxin load caused a significantly higher plasma vitamin E content but the changes were less expressed in the group subje... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572500 Thu, 01 Mar 2007 05:00:00 +0100 1572500 http://www.medworm.com/index.php?rid=1572499&cid=s_37354_80_f&fid=37354&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17385554%26dopt%3DAbstract Authors: Eren M, Uyanik F The influence of dietary boron (B) supplementation on some serum parameters and egg-yolk cholesterol was studied in laying hens. A total of 224 eighteen-week-old hens of the Hyline Brown 98 strain were assigned to 7 groups with 4 replicates of 8 hens each after 10 days of adaptation, and they were fed commercial diets supplemented with 0, 5, 10, 50, 100, 200 or 400 mg/kg (diet) B (H3BO3) for 8 weeks. Serum gamma-glutamyl transpeptidase (GGT) activity, albumin, glucose, total cholesterol, HDL- and LDL-cholesterol levels were decreased with all B levels. Except in the group receiving 5 mg/kg B supplementation, decreases were found in serum triglycerides in all groups. Serum aspartate aminotransferase (AST) activity was decreased in the groups receiving 100 mg/kg... Acta Veterinaria Hungarica journals http://www.medworm.com/rss/comments.php?id=1572499 Thu, 01 Mar 2007 05:00:00 +0100 1572499