MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Acta Biologica Hungarica' source. Fri, 27 Jan 2012 14:48:20 +0100 http://www.medworm.com/index.php?rid=5472919&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119864%26dopt%3DAbstract Authors: Tomášová L, Smajda B, Bona M Abstract The aim of this study was to assess, whether a sublethal dose of gamma-rays will influence the avoidance behaviour and anxiety in rats and whether the response to radiation depends on time of day of its application. Adult male Wistar rats were tested in elevated plus-maze, in hot plate test and in the light/dark box in 4 regular intervals during a day. After two weeks the animals were irradiated with a whole-body dose 6 Gy of gamma-rays. One day after irradiation the animals were repeatedly tested in the same way, as before irradiation. In the plus-maze test an increased level of anxiety was established. The irradiation significantly decreased the locomotor activity of rats, but the extent of exploratory and comfortable behaviour we... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472919 Thu, 01 Dec 2011 05:00:00 +0100 5472919 http://www.medworm.com/index.php?rid=5472918&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119865%26dopt%3DAbstract In this study, prolactin receptor (PRLR) was detected in active and hibernating frog dorsal skin using immunohistochemical method. PRLR immunoreactivity in active frogs was observed in the epidermis, in the secretory epithelium of granular glands and the secretory channel cells of the glands. Myoepithelial cells of granular glands that started accumulating secretory material or those with a full lumen were PRLR immunoreactive, while some myoepithelial cells of empty granular glands were negative for PRLR. In hibernating frogs, this immunoreactivity was observed in the same regions; however, immunoreactivity was more intense than that in active frogs. PCNA was employed for detection of proliferative activity of PRL in the dorsal skin, and immunoreactivity was detected in the nuclei of a few... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472918 Thu, 01 Dec 2011 05:00:00 +0100 5472918 http://www.medworm.com/index.php?rid=5472917&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119866%26dopt%3DAbstract Authors: Prakash T, Kotresha D, Rama Rao N Abstract The present study was to investigate the effect of W. calendulacea on ischemia and reperfusion-induced cerebral injury. Cerebral ischemia was induced by occluding right and left common carotid arteries (global cerebral ischemia) for 30 min followed by reperfusion for 1 h and 4 h individually. Various biochemical alterations, produced subsequent to the application of bilateral carotid artery occlusion (BCAO) followed by reperfusion viz. increase in lipid peroxidation (LPO), hydrogen peroxide (H2O2), and decrease in reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD), level in the brain tissue, Western blot analysis (Cu-Zn-SOD and CAT) and assessment of cerebral infarct size were measured. All those enzymes are ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472917 Thu, 01 Dec 2011 05:00:00 +0100 5472917 http://www.medworm.com/index.php?rid=5472916&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119867%26dopt%3DAbstract Authors: Koca YB, Gürcü B Abstract Histological structures of esophagus and stomach tissue samples of Lacerta stellio have been studied, and glycosaminoglycan (GAG) distribution has been histochemically determined. Histologically, esophagus and stomach of L. stellio are composed of four layers: mucosa, submucosa, muscularis mucosae and serosa. Mucosa of esophagus is covered by simple columnar ciliated epithelium with many mucous secreting goblet cells and contains branched tubular glands.Stomach of L. stellio is composed of fundus (oral and aboral) and pylorus regions. Mucosa is covered by columnar epithelium. Fundic glands are branched tubular glands while pyloric glands are usually simple tubular glands. In both regions of the stomach, glands are subdivided into three areas as ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472916 Thu, 01 Dec 2011 05:00:00 +0100 5472916 http://www.medworm.com/index.php?rid=5472915&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119868%26dopt%3DAbstract Authors: Aliyazicioglu Y, Demir S, Turan I, Cakiroglu TN, Akalin I, Deger O, Bedir A Abstract The aim of the present study was to evaluate the potential of Turkish propolis extracts if they prevent or protect foreskin fibroblast cells against hydrogen peroxide (H2O2)-induced oxidative DNA damage. Hydrogen peroxide (40 μM) was used as an inducer of oxidative DNA damage. The damage of DNA was evaluated by using the alkaline single cell gel electrophoresis (comet) assay. Turkish propolis extracts at concentrations of 25, 50, 75 and 100 μg/ml were prepared by ethanol. Anti-genotoxicity was assessed before, simultaneously, and after treatment of propolis extract (50 μg/ml) with H2O2. The results showed a significant decrease in H2O2-induced DNA damage in cultures treated with propoli... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472915 Thu, 01 Dec 2011 05:00:00 +0100 5472915 http://www.medworm.com/index.php?rid=5472914&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119869%26dopt%3DAbstract Authors: Pardi N, Vámos E, Ujfaludi Z, Komonyi O, Bodai L, Boros IM Abstract Using yeast two-hybrid screens we determined that Drosophila (Dm)p53 interacts with proteins involved in sumoylation (UBA2, UBC9 and PIAS) through different regions of its C-terminal domain. A K302R point mutation within a single canonical sumoylation site of Dmp53 did not abolish the observed interactions. These observations prompted us to analyze whether Dmp53 sumoylation at this site has any functional role in vivo. Genetic assays showed that deleting one copy of genes involved in sumoylation (lwr, Su(var)2-10 or smt3 heterozygosity) enhanced slightly the mutator phenotype of Dmp53. We compared the in vivo effects of wild type and K302R Dmp53 overproduced from transgenes and determined that similar lev... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472914 Thu, 01 Dec 2011 05:00:00 +0100 5472914 http://www.medworm.com/index.php?rid=5472913&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119870%26dopt%3DAbstract Authors: Faragó N, Zvara A, Varga Z, Ferdinandy P, Puskás LG Abstract Micro RNAs (miRNA) are an abundant class of small RNAs that regulate the stability and translation of cognate mRNAs. MiRNAs are potential diagnostic markers, moreover, they play an essential role in the development of various heart disesases. In case of limited tissue material, such as, e.g. human biopsies, purification of miRNAs with sufficient yield is critical. Reproducible expression analysis of miRNAs is highly dependent on the quality of the RNA, which is often difficult to achieve from fibrous tissue such as the heart. Several companies developed general purification kits for miRNAs, however, none of them are specialized to fibrotic tissues. Here we describe an optimized miRNA purification protocol that ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472913 Thu, 01 Dec 2011 05:00:00 +0100 5472913 http://www.medworm.com/index.php?rid=5472912&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119871%26dopt%3DAbstract In this study, the effects of a porphyrazine derivative, [octakis (2-trimethylammoniumethyl thio) porphyrazinatocobalt] octaiodide (CoPzq), were explored on the developmental characteristics, sex ratio and egg fertility of Drosophila melanogaster. CoPzq was preferred in the study due to its probability to be used as a chemotherapeutic agent in medicine. As a result of the application of CoPzq, a phenotypic abnormality was revealed within the individuals of F1 and F2 generation of Drosophila. Although individuals of F2 generation did not directly come into contact with the chemical, the rate of abnormality is high. An increase was observed within both generations in terms of the egg fertility. Besides, a deviation emerged in the sex-ratio in some concentrations. PMID: 22119871 [PubMed -... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472912 Thu, 01 Dec 2011 05:00:00 +0100 5472912 http://www.medworm.com/index.php?rid=5472911&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119872%26dopt%3DAbstract Authors: Mohana Priya A, Karutha Pandian S, Ramesh M Abstract A simple and efficient protocol has been developed for high frequency plant regeneration through callus cultures derived from leaf bases of abiotic stress sensitive Asian indica rice variety IR 64. Leaf base segments (4-5 mm diameter) were obtained from 6-day-old dark grown seedlings germinated on halfstrength Murashige and Skoog medium and cultured on MS medium supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2.2-18 μM) and Kinetin (0.2-1.7 μM). Among the various combinations, 13.5 μM 2,4-D and 1.3 μM Kn resulted in high callus induction frequency (87.5%) with a maximum fresh weight of 0.22 g per segment. The regeneration frequency was 75.5% with multiple shoots within 3 weeks of transfe... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472911 Thu, 01 Dec 2011 05:00:00 +0100 5472911 http://www.medworm.com/index.php?rid=5472910&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119873%26dopt%3DAbstract Authors: Csabai J, Nagy Z, Mándy AT Abstract In vitro shoot multiplication of Telekia speciosa (Schreb.) Baumg. was tested on media containing benzyladenine, benzyladenine riboside, kinetin, zeatin, meta-topolin or 2-isopentenyladenine in different concentrations. We observed the proliferation rate, the length of shoots, rate of callus formation, and the presence of the hyperhydricity. The highest proliferation rate (13.17) was obtained on medium supplemented with 5.0 μM benzyladenine, however, the leaves were hyperhydrated at this concentration of benzyladenine, therefore for shoot multiplication lower (1.0 μM BA) concentration of benzyladenine is suggested. The longest shoots were achieved using 1.0 μM 2-iP. At this treatment 100% rooting was found, therefore the stage of roo... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472910 Thu, 01 Dec 2011 05:00:00 +0100 5472910 http://www.medworm.com/index.php?rid=5472909&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119874%26dopt%3DAbstract Authors: Ozdener Y, Aydin BK, Fatma Aygün S, Yürekli F Abstract In order to determine the toxic effect of chromium Cr(VI) on the seed germination, the root and shoot length, the root-cotyledonary leaves, the fresh and dry weight in eight-day-old seedlings Brassica oleracea L. var. acephala DC (kale) were treated with various concentrations of Cr in the growth medium. The accumulation of chromium in the tissues was determined in the cotyledons and the roots of the kale seedlings. High rate of Cr uptake was observed in the roots. But the organs could not accumulate large amount Cr. The effect of Cr on B. oleracea var. acephala was evaluated by changes in chlorophyll a, b, lipid peroxidation, proline, ascorbate, protein carbonyl groups, non-protein thiols and peroxidase activity. Th... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472909 Thu, 01 Dec 2011 05:00:00 +0100 5472909 http://www.medworm.com/index.php?rid=5472908&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119875%26dopt%3DAbstract Authors: Sarmast MK, Salehi H, Khosh-Khui M Abstract The downside of plant tissue culture techniques is an unwanted microbial contamination. Elimination of contaminants is the first step of any successful investigation on plant tissue culture. Preliminary experiments on Araucaria excelsa R. Br. var. glauca (Norfolk-Island pine) (syn.: A. heterophylla) showed that most common decontaminants could not successfully eliminate the contamination. Therefore, nano silver (NS) colloids were evaluated for controlling contamination. Treatments were included soaking the explants in NS solution or adding NS to the culture medium. Explants were cultured on MS medium supplemented with appropriate growth regulators for their establishment. Results showed that surface sterilization followed by trea... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472908 Thu, 01 Dec 2011 05:00:00 +0100 5472908 http://www.medworm.com/index.php?rid=5472907&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119876%26dopt%3DAbstract Authors: Horváth L, Székely C, Boczonádi Z, Mészáros E, Bercsényi M, Urbányi B, Müller T Abstract European eel is a catadromous fish species, which means that after living in freshwater premature individuals adapt to sea water, and migrate to the Sargasso Sea for spawning. Although male eel can be sexually matured even in freshwater, to date, it was believed that female eel can be matured only in seawater. Here we show that the process of sexual maturation may be induced in freshwater by treating female eels with carp pituitary (GSI = 9.87±1.55%). It is thus proposed that seawater condition is not an obligatory environment for stimulating gametogenesis and for artificial maturation of the European eel in neither gender. PMID: 22119876 [PubMed - in process] (Source: Act... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5472907 Thu, 01 Dec 2011 05:00:00 +0100 5472907 http://www.medworm.com/index.php?rid=5139821&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840824%26dopt%3DAbstract In conclusion, quercetin both partially protects H2O2-induced gliotoxicity and decreases rat glial cell viability in primary culture. PMID: 21840824 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139821 Fri, 19 Aug 2011 12:28:47 +0100 5139821 http://www.medworm.com/index.php?rid=5139820&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840825%26dopt%3DAbstract Authors: Csaba G, Pállinger E Abstract The aim of the experiments was to study the regulation of triiodothyronine (T3) production in the unicellular Tetrahymena. Untreated and troph-hormone treated specimen were prepared and in different timepoints T3 content was measured and compared by immunocytochemical flow cytometry. 0.1 or 0.001 IU TSH in tryptone-yeast medium stimulated T3 synthesis at 10, 20, 30 min, but does not stimulate after 1 h. The overlapping gonadotropic hormone (GTH) also did it, however only at 10 min. In Losina salt solution (physiological for Tetrahymena) the effect was weaker, however outer amino acid source was not absolutely needed for the production of the hormone. The results show that the TSH regulation of thyroid hormone synthesis (storage, secretion) an... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139820 Fri, 19 Aug 2011 12:28:44 +0100 5139820 http://www.medworm.com/index.php?rid=5139819&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840826%26dopt%3DAbstract In conclusion, the present investigation demonstrates for the first time that components in T. cucumerina aerial parts can exert significant antioxidant activity in vivo and in vitro. PMID: 21840826 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139819 Fri, 19 Aug 2011 12:28:40 +0100 5139819 http://www.medworm.com/index.php?rid=5139818&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840827%26dopt%3DAbstract Authors: Kim MJ, Kim HK Abstract The present study was performed to examine the effects of garlic on obesity and blood lipid profiles in high-fat induced obesity mice model, and to elucidate the molecular mechanisms responsible for such effect. C57BL/6 mice were fed a standard diet (STD) or high-fat diet (HFD) for 5 weeks to induce obesity. Mice were then randomly divided into four groups with 10 mice per group, and fed experimental diet for 4 weeks; STD group, HFD group, HFD containing 2% or 4% garlic group (HFD + G2 or HFD + G4, respectively). Administration of garlic significantly reduced HFD-induced body weight, epididymal fat accumulation, hyperlipidemia and hypercholesterolemia. Consequently, the atherogenic indexes were reduced by 83% and 91%, respectively, in 2% and 4% garl... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139818 Fri, 19 Aug 2011 12:28:36 +0100 5139818 http://www.medworm.com/index.php?rid=5139817&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840828%26dopt%3DAbstract Authors: Murber A, Fancsovits P, Ledó N, Szakács M, Rigó J, Urbancsek J Abstract The quality of oocytes and developing embryos are the most relevant factors determining the success of an in vitro fertilization (IVF) treatment. However, there are very few studies analyzing the effects of different gonadotrophin preparations on oocyte and embryo quality. A retrospective secondary analysis of data collected from a prospective randomized study was performed to compare highly purified versus recombinant follicle stimulating hormone (HP-FSH vs. rFSH). The main outcome measures were quantity and quality of oocytes and embryos, dynamics of embryo development, cryopreservation, clinical pregnancy and live birth rate. The number of retrieved and of mature (MII) oocytes showed no significa... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139817 Fri, 19 Aug 2011 12:28:32 +0100 5139817 http://www.medworm.com/index.php?rid=5139816&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840829%26dopt%3DAbstract Authors: Horbowicz M, Wiczkowski W, Koczkodaj D, Saniewski M Abstract The jasmonates, which include jasmonic acid and its methyl ester (MJ), play a central role in regulating the biosynthesis of many secondary metabolites, including flavonoids, and also are signaling molecules in environmental stresses. Synthesis of anthocyanins pigments is a final part of flavonoids pathway route. Accumulation of the pigments in young seedlings is stimulated by various environmental stresses, such as high-intensity light, wounding, pathogen attack, drought, sugar and nutrient deficiency. The anthocyanins take part in defense system against excess of light and UV-B light, and therefore it is probably main reason why young plant tissues accumulate enlarged levels of the pigments. The effects of exog... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139816 Fri, 19 Aug 2011 12:28:28 +0100 5139816 http://www.medworm.com/index.php?rid=5139815&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840830%26dopt%3DAbstract Authors: Canakci S, Dursun B Abstract The present study examined the effects of salicylic acid pre-application on the responses of seven-day-old chickpea (Cicer arietinum L.) seedlings to nickel. For this purpose, the plants were treated with 1 mM salicylic acid solution for 6 and 10 hours and then treated with 0.75, 1.5 and 3 mM nickel solutions for 48 hours hydroponically. Following the treatment, changes in seedling length, seedling fresh weight and leaf dry weight (after 10 hours), as well as MDA, proline, protein and pigment contents (after 6 and 10 hours) were examined. Salicylic acid pre-application was found to significantly alleviate the typical harmful effects caused by nickel and 3 mM nickel concentration in particular, on the parameters associated with toxic stress. How... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139815 Fri, 19 Aug 2011 12:28:24 +0100 5139815 http://www.medworm.com/index.php?rid=5139814&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840831%26dopt%3DAbstract Authors: Truta E, Vochita G, Rosu CM, Zamfirache MM, Olteanu Z Abstract The study was performed in order to evaluate Roundup-induced genotoxic effects in Hordeum vulgare L. cv. Madalin root meristems and to analyze herbicide impact on length growth of barley seedlings. Caryopses were treated for 3 hours and 6 hours with 0.1%, 0.5%, 1.0% and 2.0% Roundup solutions (v/v), containing 0.36 mg ml-1, 1.8 mg ml-1, 3.6 mg ml-1 and 7.2 mg ml-1 glyphosate active ingredient. Mitotic index decreased in both exposure times with concentration increase. In 3-h treatment, its average values decreased from 4.73 ± 0.31% to 1.51 ± 0.43%, whereas in 6-h treatment this parameter declined from 3.86 ± 0.92% to 0.62 ± 0.15%. The highest ana-telophase aberration rates were noted in 3-h treatments (8.91... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139814 Fri, 19 Aug 2011 12:28:20 +0100 5139814 http://www.medworm.com/index.php?rid=5139813&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840832%26dopt%3DAbstract This study shows the tolerance of C. maritimum to the phosphorus deprivation combined to moderate or to high saline level which may explain the common patterns of colonization and zonation of this species. PMID: 21840832 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139813 Fri, 19 Aug 2011 12:28:16 +0100 5139813 http://www.medworm.com/index.php?rid=5139812&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840833%26dopt%3DAbstract The objectives of this study were to elucidate the impact of bird cherry-oat aphid (Rhopalosiphum padi L.) feeding on functioning of the proteolytic machinery in bird cherry leaves. Biochemical analyses proved that R. padi feeding in tissues of primary host stimulated activity of the two major fractions of proteinases (extracted at the optimal pH values: 5.0 and 7.0). Additionally, it has been demonstrated that aphids' feeding on bird cherry led to a decline in levels of albumins and globulins (main protein fractions in P. padus leaves). The opposite tendency, regarding the amounts of these protein fractions was ascertained at the phase of disappearance of R. padi population on tested shoots. Furthermore, it is reported that an increase in activity of the analysed enzymes and a decline in ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139812 Fri, 19 Aug 2011 12:28:12 +0100 5139812 http://www.medworm.com/index.php?rid=5139811&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840834%26dopt%3DAbstract Authors: Chaveerach A, Tanee T, Sudmoon R Abstract Nymphaea species, the most popular decorative plants, were collected for specificity of inter-simple sequence repeat (ISSR) analyses in species identification and differentiation of cultivars and natural populations. Dendrogram constructed from ISSR analyses separated out wild species, namely Nymphaea cyanea, N. nouchali, N. capensis, N. lotus and an outgroup N. mexicana, and cultivars. The dendrogram indicates that the cultivars should be differentiated from N. capensis, as they are sister individuals of N. capensis. The ISSR banding data and the dendrogram are concordantly concluded that wild N. capensis would be an effective type species for producing different cultivars. After plant identification by ISSR markers, DNA barcodes ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5139811 Fri, 19 Aug 2011 12:28:08 +0100 5139811 http://www.medworm.com/index.php?rid=4853286&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555263%26dopt%3DAbstract Authors: Kozlu T, Karadag Sari E, Akaydin Bozkurt Y, Altunay H The spleen structurally and functionally belongs to the hematopoietic organs and is also an important component of the reticuloendothelial system, which is known to play a major role in host defense. The histological structure of the spleen was investigated in the ostrich, a non-flying bird, the kestrel, a raptor, and the osprey, a fish-eating bird of prey (fish eagle). For this purpose, Mallory's modified triple stain, methyl green-pyronin and silver stain were used. Germinal centers were not present in the spleen of the osprey. In the spleen of the kestrel, penicillar arterioles and the surrounding lymphoid tissue were markedly dense. Compared to the other two birds, the red and white pulps were clearly distinguishable in... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853286 Mon, 23 May 2011 20:31:43 +0100 4853286 http://www.medworm.com/index.php?rid=4853285&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555264%26dopt%3DAbstract In conclusion, differences in these two experimental models for reticulocytosis may be used as tools for appropriate pharmacological testing of redox-active substances considering energy and redox processes, as well as apoptosis pathways. PMID: 21555264 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853285 Mon, 23 May 2011 20:31:40 +0100 4853285 http://www.medworm.com/index.php?rid=4853284&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555265%26dopt%3DAbstract Authors: Szaroma W, Dziubek K Reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx) are vital components of the antioxidative barrier in animal cells. It is suggested more often now that the effectiveness of the protection of cells against the oxidative stress caused by the inflammation process depends on the amount of GSH and the activity of SOD, CAT and GSHPx. That is why the effect of zymosan A (40 mg/kg body mass) and the combined treatment with zymosan A (at the same dose) and melatonin (50 mg/kg body mass) on the amount of GSH in the blood and the amount of GSH and activity of SOD, CAT and GSHPx in the brain, liver and kidneys of male mice was estimated. Animals (n = 108) were decapitated after 3, 6 and 24 hours since the moment ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853284 Mon, 23 May 2011 20:31:36 +0100 4853284 http://www.medworm.com/index.php?rid=4853283&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555266%26dopt%3DAbstract Authors: Wang Y, Dai Y, Li X, Chen CY, Li W, Yu Z The effect of all-trans retinoic acid (atRA) on palatal fusion and the underlying mechanisms were investigated using organ culture. Compared with control group, the atRA-treated group (1 μM and 5 μM) had more medial edge epithelium (ME) remaining within the midline epithelial seam (MES). At 10 μM atRA, the opposing shelves were not in contact at the culture end (72 h). Cell death detection by TUNEL and laminin immunohistochemistry demonstrated that atRA (5 μM) induced apoptosis in mesenchyme and inhibited degradation of basal lamina within MES. Notably, migration and apoptosis of ME cells and degradation of basal lamina within MES markedly represented vehicle control palatal shelves in culture. Additionally, apoptosis was not detect... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853283 Mon, 23 May 2011 20:31:32 +0100 4853283 http://www.medworm.com/index.php?rid=4853282&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555267%26dopt%3DAbstract Authors: Katsuragi H, Shimoda K, Yamamoto R, Ohara T, Hamada H Capsaicin 4-O-β-xylooligosaccharides were synthesized by a biocatalytic xylosylation using Aspergillus sp. β-xylosidase. Capsaicin was converted into three new capsaicin glycosides, i.e. capsaicin 4-O-β-xyloside, capsaicin 4-O-β-xylobioside, and capsaicin 4-O-β-xylotrioside in 15, 12 and 10% yield, respectively. All products were isolated from the reaction mixtures by preparative HPLC. The structures of the products were determined by NMR spectroscopic method. PMID: 21555267 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853282 Mon, 23 May 2011 20:31:28 +0100 4853282 http://www.medworm.com/index.php?rid=4853281&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555268%26dopt%3DAbstract In conclusion, aluminum is significantly stressful in maize culminating in morphological and cellular alterations. PMID: 21555268 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853281 Mon, 23 May 2011 20:31:24 +0100 4853281 http://www.medworm.com/index.php?rid=4853280&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555269%26dopt%3DAbstract The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosace... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853280 Mon, 23 May 2011 20:31:20 +0100 4853280 http://www.medworm.com/index.php?rid=4853279&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555270%26dopt%3DAbstract Authors: Smaoui A, Jouini J, Rabhi M, Bouzaien G, Albouchi A, Abdelly C Cotula coronopifolia is a wild annual Asteraceae that grows in periodically-flooded prone environments and seems highly tolerant to periodic flooding. Seedlings of about 15 cm were collected directly from the edge of Soliman sabkha (N-E Tunisia, semi-arid stage) and grown under greenhouse conditions. Two treatments were considered: drainage and flooding. After 56 days of treatment, flooded plants showed a pronounced growth increase. This performance was essentially associated with significant increment in biomass production of both shoots and roots (about 220% of the control). The appropriate response to flooding was also characterized by the ability of the species to maintain its water status under such conditions... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853279 Mon, 23 May 2011 20:31:16 +0100 4853279 http://www.medworm.com/index.php?rid=4853278&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555271%26dopt%3DAbstract Authors: Mansour HA, Shoman SA, Kdodier MH Five cyanophyte species (Amorphonostoc punctiforme, Gloeocapsa turgidus, Sphaeronostoc coeruleum, Stratonostoc linckia f. spongiaeforme and Synechococcus cedrorum) were isolated and identified from sandy Egyptian soils. Polysaccharides extracted from these species showed a pronounced antiviral activity against Rabies and Herpes-1 viruses represented by the absence of the characteristic cytopathic effects of these viruses. It was found that 100 μg polysaccharide/ml induced 100% inhibition of the two viruses which, depending on the polysaccharide concentration. Both of Gloeocapsa turgidus and Synechococcus cedrorum showed higher antiviral activity against rabies virus than that against herpes-1 virus. Amorphonostoc punctiforme showed nil to wea... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853278 Mon, 23 May 2011 20:31:11 +0100 4853278 http://www.medworm.com/index.php?rid=4853277&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555272%26dopt%3DAbstract Authors: Horváth E, Papp G, Gazdag Z, Belágyi J, Blaskó A, Deli J, Vágvölgyi C, Pesti M A carotenoid-less Phaffia rhodozyma mutant (MCP 325) exhibited significantly higher resistance to oxidative stressors such as menadione, H2O2 and K2Cr2O7 than its astaxanthin-producing parental strain (MCP 324). The absence of carotenoids in the mutant did not explain this phenomenon. The cause of the decreased superoxide, hydroxyl radical and glutathione contents, the increased peroxide concentration and the elevated specific activity of catalase under uninduced conditions may be a second mutation. Peroxide treatment induced specific catalase activity in the mutant but not in the parental strain. Regulation of these processes led to the result that, in spite of the mutations, the two strains e... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853277 Mon, 23 May 2011 20:31:07 +0100 4853277 http://www.medworm.com/index.php?rid=4853276&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555273%26dopt%3DAbstract This study indicated that transient sodium conductance plays an indispensable role in pacemaking, which occurs under conditions in which only a persistent sodium conductance is considerably reduced, thus contributing to a detailed understanding of the relationship between sodium conductance and pacemaking. PMID: 21555273 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853276 Mon, 23 May 2011 20:31:00 +0100 4853276 http://www.medworm.com/index.php?rid=4853275&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555274%26dopt%3DAbstract Authors: Ghomi MR, Zarei M, Sohrabnejad M Because of the effect of photoperiod on physiological and biochemical processes in fish, this study aimed to evaluate the effect of manipulated photoperiod on growth, feed conversion and survival of wild carp, Cyprinus carpio. Fish received six photoperiod regimes (light:dark cycle) including: natural photoperiod (control), 24L:0D, 16L:8D, 12L:12D, 8L:16D and 0L:24D by the three replications. Regulated photoperiods as a 16L:8D or 12L:12D light/dark cycle significantly improved growth rate and food conversion ratio of wild carp. PMID: 21555274 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853275 Mon, 23 May 2011 20:30:50 +0100 4853275 http://www.medworm.com/index.php?rid=4853274&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21555275%26dopt%3DAbstract Authors: Szilágyi M PMID: 21555275 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4853274 Mon, 23 May 2011 20:30:35 +0100 4853274 http://www.medworm.com/index.php?rid=4574930&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388915%26dopt%3DAbstract Authors: Péczely P, Bogenfürst F, Kulcsár M, Polgár B Plasma levels of testosterone (T), 17-β-estradiol (E2), progesterone (P4), dehydroepiandrosterone (DHEA), corticosterone (B), thyroxine (T4) and triiodothyronine (T3) were monitored during postnuptial and the prenuptial molt in domestic goose (Anser anser domesticus) in both sexes. 1. At the beginning of postnuptial molt (when the old, worn dawny-, and cover feathers' loss starts) in ganders, the levels of T, E2, P4 decrease while DHEA and B significantly increase. The elevated levels of T4 and low T3 concentrations characteristic of the last phase of the reproduction, remain unchanged. In layers, similar changes were observed, however, B decreases. 2. In the early phase of outgrowth of wing and cover feathers, plasma levels of... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574930 Tue, 01 Mar 2011 00:00:00 +0100 4574930 http://www.medworm.com/index.php?rid=4574929&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388916%26dopt%3DAbstract Authors: Başkurt SI, Doğaç E, Taşkın V, Taşkın BI In the present study, the frequencies of three organophosphate (OP) resistance-associated mutations in acetylcholinesterase gene of Bactrocera oleae (BoAce) populations collected from 8 different important olivegrowing areas in the west part of Turkey were determined. Populations were sampled from the areas that have been treated with only the pyrethroid α-cypermethrin; pyrethroids plus OPs; deltamethrin with pheromone eco-traps, and no insecticide treatment applied areas for many years. For Ile214Val and Gly488Ser point mutations PCR-RFLP and for Δ3Q deletion mutation PCR diagnostic tests were carried out. Seventy-two percent of the total individuals analyzed in the study were exhibited heterozygous genotype (RS) for both Ile2... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574929 Tue, 01 Mar 2011 00:00:00 +0100 4574929 http://www.medworm.com/index.php?rid=4574928&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388917%26dopt%3DAbstract We report a high frequency regeneration protocol in cowpea (Vigna unguiculata Walp. var. C 152) via somatic embryogenesis from 10-d-old primary leaf explants. A study was conducted to examine the effect of somaclonal variations in in vitro derived cowpea plants under field conditions. The regenerated plantlets were successfully transferred to field after hardening in vitro and grown for collecting R0, R1 and R2 seeds. The seeds of R1 and R2 generations were subsequently, grown under field conditions and their various biometrical traits were compared and evaluated with non-tissue cultured cowpea plants as check. There was no detectable somaclonal variation induced in R0-R2 in any of the biometrical traits. The results indicate that the inclusion of different plant growth promoters at specif... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574928 Tue, 01 Mar 2011 00:00:00 +0100 4574928 http://www.medworm.com/index.php?rid=4574927&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388918%26dopt%3DAbstract Authors: Jahan AA, Anis M, Aref IM Rapid differentiation of multiple shoots was observed in 94% of nodal explants of one year old Nyctanthes arbor-tristis L. plants. Shoot bud induction and multiplication took place on Murashige and Skoog (MS) medium supplemented with two cytokinins, i.e. Benzyladenine (BA) or Kinetin (Kn) either alone or in combination with different auxins, indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) or α-naphthalene acetic acid (NAA). Between different media, pH levels and growth regulators tried, the optimum condition for maximum regenerative response was obtained on MS + Kn (2.5 μM) + N AA (0.5 μM) media at 5.8 pH, forming cultures with 23.26 ± 0.89 number of shoots and 6.36 ± 0.80 cm shoot length after 8 weeks of culture. Histological sections co... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574927 Tue, 01 Mar 2011 00:00:00 +0100 4574927 http://www.medworm.com/index.php?rid=4574926&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388919%26dopt%3DAbstract Authors: Keszthelyi S, Pál-Fám F, Kerepesi I The cotton bollworm (Helicoverpa armigera Hübner), which migrated in the Carpathian-basin from Mediterraneum in the last decades, is becoming an increasingly serious problem for maize producers in Hungary. In several regions the damage it causes has reached the threshold of economic loss, especially in the case of the sweet maize cultivation. The aim of the research was to determine the changing of ears weights and in-kernel accumulation and alteration in grain as a function of cotton bollworm mastication.Our investigation confirmed that there is an in-kernel and protein pattern change of maize grain by cotton bollworm. Our results proved the significant damaging of each part of ears by cotton bollworm masticating (the average weight loss... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574926 Tue, 01 Mar 2011 00:00:00 +0100 4574926 http://www.medworm.com/index.php?rid=4574925&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388920%26dopt%3DAbstract Authors: Maróti-Agóts A, Bodó I, Jávorka L, Gyurmán A, Solymosi N, Zenke P, Skogseth M, Zöldág L The synthesis of Heat Shock Protein 70.2 mRNA is also regulated by the Upper Promoter elements of the gene. This promoter region is polymorphic in cattle. These polymorphisms have a major effect on the activity of the mRNA transcription. In a comparison of quantity of transcribed mRNA from the wild type and AP2 mutant allele the wild type can produce 2-3-fold more transcripts.The Hungarian Grey Cattle (HG) and Norwegian Red (NFR) as control breed were genotyped with PCR-RFLP method. Our results showed that the frequencies of alleles in breeds (p(wt)HG = 0.859419, p(wt)NFR = 0.5) are different. The effective response to heat stress in the Norwegian Red seems to be less important than ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574925 Tue, 01 Mar 2011 00:00:00 +0100 4574925 http://www.medworm.com/index.php?rid=4574924&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388921%26dopt%3DAbstract In conclusion, SCAR (sequence characterized amplified regions) markers were successfully identified and rapid, reliable tools were provided for the detection of an effective biocontrol Trichoderma strain, which can facilitate studies of its population dynamics and establishment after release into the natural environment. PMID: 21388921 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574924 Tue, 01 Mar 2011 00:00:00 +0100 4574924 http://www.medworm.com/index.php?rid=4574923&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388922%26dopt%3DAbstract Authors: Fawzi EM A thermostable xylanase was purified and characterized from the thermophilic fungus Rhizomucor miehei (Cooney & Emerson) Schipper. The enzyme was purified to homogeneity by ammonium sulfate precipitation, sephadex G-100 gel filtration and diethylaminoethyl cellulose anion exchange chromatography with a 29.1-fold. The enzyme was highly active within a range of pH from 5.0 to 6.5. The optimum temperature of the purified enzyme was 75 °C. The enzyme showed high thermal stability at 70 °C and 75 °C and the half-life of the xylanase at 90 °C was 30 min. Km and Vmax values at 50 °C of the purified enzyme were 0.055 mg/ml and 113.5 μmol min-1 mg-1, respectively. The enzyme was activated by Ca2+, Cu2+, K+ and Na+. On the other hand, Ag2+, Hg2+, Ba2+, and Zn2+ inhibi... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574923 Tue, 01 Mar 2011 00:00:00 +0100 4574923 http://www.medworm.com/index.php?rid=4574922&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388923%26dopt%3DAbstract Authors: Uymaz B, Akkoç N, Akçelik M The probiotic characteristics of Lactobacillus brevis BG18 and Lb. plantarum BG33, isolated from traditional Turkish Tulum cheese were assessed. These two bacteriocinproducer strains exhibited good probiotic characteristics such as resistance in media containing 0.3% bile salt, pepsin (3 mg mL-1), and pancreatine (1 mg mL-1) as well as acid resistance at pH 2. They were also adhered to Caco-2 epithelial cells in a manner comparable to Escherichia coli LMG3083 (ETEC) and Salmonella Typhimurium SL1344. The strains produced a heat-stable antimicrobial compound that was shown to be proteinaceous in nature, and therefore, referred to as bacteriocins. The bacteriocins were able to inhibit growth of a number grampositive bacteria such as Listeria monocyt... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574922 Tue, 01 Mar 2011 00:00:00 +0100 4574922 http://www.medworm.com/index.php?rid=4574921&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21388924%26dopt%3DAbstract Authors: Blaskó A, Fajka-Boja R, Ion G, Monostori E Galectin-1 (Gal-1), a mammalian lectin induces apoptosis of T lymphocytes. Contradictory data have resulted in confusing knowledge regarding mechanism of Gal-1 induced T-cell apoptosis. In this paper we aimed to resolve this controversy by comparing cell death induced by low (1.8 μM, lowGal-1) and high (18 μM, highGal-1) concentration of soluble Gal-1. We show that lowGal-1 and highGal-1 trigger phosphatidylserine exposure, generation of rafts and mitochondrial membrane depolarization. In contrast, lowGal-1 but not highGal-1 is dependent on the presence of p56lck and ZAP70 and activates caspase cascade. The results allow the conclusion that the cell-death mechanism strictly depends on the concentration of Gal-1. PMID: 21388924 ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4574921 Tue, 01 Mar 2011 00:00:00 +0100 4574921 http://www.medworm.com/index.php?rid=4215457&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112829%26dopt%3DAbstract Authors: Gallyas F Enigmatic morphological features of the formation and fate of "dark" (hyper-basophilic, hyper-argyrophilic and hyper-electrondense) neurons suggest that the mechanical work causing their dramatic shrinkage (whole-cell ultrastructural compaction) is done by a previously "unknown" ultrastructural component residing in the spaces between their "known" (i.e. visible in the conventional transmission electron microscopy) ultrastructural constituents. Embedment-free section electron microscopy revealed in these spaces the existence of a continuous network of gel microdomains, which is embedded in a continuous network of fluid-filled lacunae. We gathered experimental facts suggesting that this gel network is capable of a volume-reducing phase-transition (an established physi... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215457 Wed, 01 Dec 2010 00:00:00 +0100 4215457 http://www.medworm.com/index.php?rid=4215456&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112830%26dopt%3DAbstract Authors: Karakaş A, Kaya A, Gündüz B We studied the effects of melatonin and leptin hormones on ovarian follicular development in intact and pinealectomized female Syrian hamsters. We first monitored the oestrous cycle of the hamsters by the vaginal smear samples throughout a ten day period to start the injections simultaneously in all groups and performed saline, melatonin and leptin hormone injection groups for both control and pinealectomized hamsters. Then the injections were applied for four days starting the oestrus phase of the cycle and the ovaries were removed for preparation of histological analysis. We measured the diameters and the numbers of the follicles and we classified the follicles according to the number of the granulosa cell layer. Leptin hormone injection increa... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215456 Wed, 01 Dec 2010 00:00:00 +0100 4215456 http://www.medworm.com/index.php?rid=4215455&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112831%26dopt%3DAbstract Authors: Garcia MT, Hermosa Y, Aguirre JI We compared the haematological and biochemical values within a population of yellow-legged gulls (Larus michahellis) in the Chafarinas Islands (Northern Africa), in non-breeding (February) and breeding (May) animals. We collected blood samples from 51 adults. We found that according to the haematological data, there was a significant variation in haemoglobin content, and a higher proportion of heterophils, thrombocytes, and Haemoproteus infection in breeding individuals with a lower level in basophils. Blood biochemistry showed a higher level in plasmatic proteins, calcium, phosphorus, thiobarbituric acidreactive substances and alkaline phosphatase as well as alanine aminotransferase activity in breeding animals while cholesterol and phospholip... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215455 Wed, 01 Dec 2010 00:00:00 +0100 4215455 http://www.medworm.com/index.php?rid=4215454&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112832%26dopt%3DAbstract Authors: Vehovszky A, Szabó H, Acs A, Győri J, Farkas A (Artemia) nauplii was used to asses the toxicity of rotenone, MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), MP+ (1-methyl-4-phenylpyridinium) and the effect of L-DOPA co-treatment with rotenone. Rotenone had a dose dependent effect on mortality (LC50: 0.37 ± 0.04 μM mean ± S E, n = 24), while MPTP and MP+ proved to be toxic in millimolar range (LC50: 0.21 ± 0.09 mM and 0.20 ± 0.08 mM, respectively, n = 4). L-DOPA (50-200 μM) co-treatment increased the survival of the rotenone-treated animals (LC50: 0.51 ± 0.23 μM, 1.03 ± 0.66 μM, and 0.76 ± 0.52 μM, respectively). In the whole body tissue homogenates of Artemia, sublethal (up to 0.3 μM) concentrations of rotenone increased the glutathione S-transferase (GST) ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215454 Wed, 01 Dec 2010 00:00:00 +0100 4215454 http://www.medworm.com/index.php?rid=4215453&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112833%26dopt%3DAbstract Authors: Orosová M, Holečková B, Siviková K, Dianovský J The effect of the fungicide Euparen Multi (containing 50% tolylfluanid) was investigated on the induction of chromosomal aberrations (CA) in cultured bovine peripheral lymphocytes. Cultures from two healthy donors were treated with tolylfluanid-based fungicide at concentrations ranging from 1.7 to 17.5 μg/ml for the last 24 and 48 hours of cultivation. Conventional cytogenetic method (CA assay) with Giemsa staining as well as fluorescence in situ hybridization (FISH) with whole bovine chromosomes 1 and 5 painting probes were used in the experiment. In the CA assay, no clastogenic effect of the fungicide was found after Euparen Multi treatment for 24 hours. On the contrary, significant elevation in polyploidy induction was o... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215453 Wed, 01 Dec 2010 00:00:00 +0100 4215453 http://www.medworm.com/index.php?rid=4215452&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112834%26dopt%3DAbstract In conclusion, low-level concentrations chronically administered exert certain effects on the levels of selected enzymes, e.g. biomarkers. PMID: 21112834 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215452 Wed, 01 Dec 2010 00:00:00 +0100 4215452 http://www.medworm.com/index.php?rid=4215451&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112835%26dopt%3DAbstract This study would add some new information about those transcriptional changes that are involved in the delayed phase of cardiac protection. PMID: 21112835 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215451 Wed, 01 Dec 2010 00:00:00 +0100 4215451 http://www.medworm.com/index.php?rid=4215450&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112836%26dopt%3DAbstract Authors: Katsuragi H, Shimoda K, Ohiro A, Hamada H The efficient production of β-glycosides of capsaicin and 8-nordihydrocapsaicin by cultured cells of Panax ginseng is reported. Capsaicin 4-O-(6-O-β-D-xylopyranosyl)-β-D-glucopyranoside (β-primeveroside, 12%) together with capsaicin 4-O-β-D-glucoside (6%) was isolated from the cell suspension of P. ginseng after one week of incubation with capsaicin. On the other hand, 8-nordihydrocapsaicin was glycosylated to 8-nordihydrocapsaicin 4-O-β-D-glucoside (5%) and 8-nordihydrocapsaicin 4-O-β-primeveroside (9%) by P. ginseng. Pretreatment of the cultured cells with salicylic acid greatly enhanced the glucosylation activity toward capsaicinoids. When 500 μM of salicylic acid was added to the cultures prior to the addition of substrate,... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215450 Wed, 01 Dec 2010 00:00:00 +0100 4215450 http://www.medworm.com/index.php?rid=4215448&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112837%26dopt%3DAbstract Authors: Várallyay E, Lichner Z, Sáfrány J, Havelda Z, Salamon P, Bisztray G, Burgyán J Medicago truncatula, the model plant of legumes, is well characterized, but there is only a little knowledge about it as a viral host. Viral vectors can be used for expressing foreign genes or for virus-induced gene silencing (VIGS), what is a fast and powerful tool to determine gene functions in plants. Viral vectors effective on Nicotiana benthamiana have been constructed from a number of viruses, however, only few of them were effective in other plants. A Tobamovirus, Sunnhemp mosaic virus (SHMV) systemically infects Medicago truncatula without causing severe symptoms. To set up a viral vector for Medicago truncatula, we prepared an infectious cDNA clone of SHMV. We constructed two VIGS vecto... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215448 Wed, 01 Dec 2010 00:00:00 +0100 4215448 http://www.medworm.com/index.php?rid=4215446&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112838%26dopt%3DAbstract Authors: Labidi N, Snoussi S, Ammari M, Metoui W, Ben Yousfi N, Hamrouni L, Abdelly C The aim of this study was to identify the relationship between the adaptive processes of Suaeda fruticosa for Pi acquisition and the physic-chemical and biological characteristics of two soil types under moderate and high saline conditions. Four treatments were established in pots: namely SS100, SS600, CS100 and CS600 where SS stood for sandy soil and CS for calcareous soil, and the indexes 100 and 600 were NaCl concentrations (mM) in irrigation distilled water. Assuming that Pi per g of plant biomass is an indicator of plant efficiency for P acquisition, the results showed that Pi acquisition was easiest on SS100 and was difficult on CS100. The differences in Pi acquisition between plants on SS100 an... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215446 Wed, 01 Dec 2010 00:00:00 +0100 4215446 http://www.medworm.com/index.php?rid=4215444&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112839%26dopt%3DAbstract Authors: Rabhi M, Hajji S, Karray-Bouraoui N, Giuntini D, Castagna A, Smaoui A, Ranieri A, Abdelly C In the present investigation, we studied uptake and management of the major cations in the xerohalophyte, Tecticornia indica (Willd.) subsp. indica as subjected to salinity. Plants were grown under greenhouse conditions at various salinity levels (0, 100, 200 and 400 mM NaCl) over 110 days. At harvest, they were separated into shoots and roots then analyzed for water contents, dry weights (DW), and Na+, K+, Ca2+, and Mg2+ contents. Plants showed a growth optimum at 200 mM NaCl and much better tissue hydration under saline than non-saline conditions. At this salt concentration (200 mM NaCl), shoot Na+ content reached its highest value (7.9 mmol · g-1 DW). In spite of such stressful cond... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215444 Wed, 01 Dec 2010 00:00:00 +0100 4215444 http://www.medworm.com/index.php?rid=4215441&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112840%26dopt%3DAbstract Authors: Kiss-Bába E, Pánczél S, Velich I, Bisztray GD Nine genotypes of melon (Cucumis melo L.) were selected for the investigation of regeneration. Most of the tested varieties showed regeneration ability on medium containing 0.5 mg l-1 or 1 mg l-1 BA, but following the appearance of shoot buds, only six varieties produced leafy shoots. The effect of combinations of BA with different auxins (IAA, NA, 2,4-D) and ABA in the culture medium on shoot regeneration was tested on cotyledon explants of 'Hógolyó' and 'Hale's Best'. To establish optimal conditions for the adventitious shoot induction six types of seedling-derived explants were prepared from seedlings of four different ages. The best results for shoot forming capacity were achieved with cotyledons followed by decapitated se... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215441 Wed, 01 Dec 2010 00:00:00 +0100 4215441 http://www.medworm.com/index.php?rid=4215437&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112841%26dopt%3DAbstract Authors: Sempruch C, Leszczyński B, Wójcicka A, Makosz M, Matok H, Chrzanowski G Changes in lysine decarboxylase (LDC) activity caused by Sitobion avenae (F.) feeding on two winter triticale cultivars (cvs) were studied. The aphid fecundity and values of intrinsic rate of natural increase showed that cv Witon was less susceptible to S. avenae than cv Tornado. The grain aphid feeding on more susceptible triticale caused a decrease in the LDC activity, with exceptions of root tissues after two weeks of the feeding. In case of less susceptible cv Witon reduction of the LDC activity was observed only during initial period of S. avenae feeding. Later the aphid infestation induced activity of the LDC within tissues of cv Witon. PMID: 21112841 [PubMed - in process] (Source: Acta Biologi... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4215437 Wed, 01 Dec 2010 00:00:00 +0100 4215437 http://www.medworm.com/index.php?rid=3890713&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724271%26dopt%3DAbstract Authors: Pintér O, Péczely P Birds from the temperate and cold zones show annual sexual activity accompanied by gonadal changes and fluctuation in their brain gonadotropin-releasing hormone (GnRH) levels. However, most of the studies were done on captive birds where the constant environment can profoundly modify periodical changes. Therefore our aim was to reveal annual variations of hypothalamic and gonadal changes in male, free-living European starlings (Sturnus vulgaris) captured directly from their natural environment. We analyzed hypothalamic GnRH-I immunoreactivity and testes volume. Four key time points of the active reproductive cycle and the photorefractory phase were studied. GnRH-I immunoreactivity was analyzed in the preoptic area (POA) and the median eminence (ME). P... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890713 Sun, 22 Aug 2010 18:45:44 +0100 3890713 http://www.medworm.com/index.php?rid=3890712&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724272%26dopt%3DAbstract This study was designed to investigate the effects of XA fruit methanol extract on oxidative stress in brain of rats exposed to whole body gamma-radiation (5 Gy). Vitamin C (VC) served as standard antioxidant. Forty-four rats were divided into 4 groups of 11 rats each. One group served as control, two different groups were treated with XA and VC (250 mg/kg), 6 weeks before and 8 weeks after irradiation, and fourth group was only irradiated. Rats were sacrificed 1 and 8 weeks after irradiation. The antioxidant status, viz. Lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST) and glutathione (GSH) were estimated. Results indicate a significant increase ( p < 0.05) in levels of brain LPO after irradiation. LPO increased by 90% and 151%, afte... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890712 Sun, 22 Aug 2010 18:45:41 +0100 3890712 http://www.medworm.com/index.php?rid=3890711&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724273%26dopt%3DAbstract Authors: Deér AK, Henczová M, Banka L, Varanka Z, Nemcsók J The effects of crude oil (Szeged-Algyo, Hungary) and oil fractions (F1: rich in aromatics; F2 fraction: free from aromatics) were investigated on liver CYP1A isoenzymes and antioxidant defence system following their i.p. injection into different freshwater fish species: carp ( Cyprinus carpio L.), silver carp ( Hyphothalmichtys molitrix V.), and European eel ( Anquilla anquilla ). A dose of 2 mL kg -1 crude oil enhanced EROD activity 8-fold in carp and only 5-fold in eel after 3 days. Oil fraction F1 caused only a 2-fold induction in EROD activity only in carp, while F2 fraction caused significant inhibition in all three investigated fish species. The antioxidant parameters [lipid peroxidation (LP), catalase (CAT), su... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890711 Sun, 22 Aug 2010 18:45:37 +0100 3890711 http://www.medworm.com/index.php?rid=3890710&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724274%26dopt%3DAbstract Authors: Szécsi M, Ondré D, Tóth I, Magony S, Wölfling J, Schneider G, Julesz J The 5alpha-reductase type 1 isozyme is a key enzyme in the metabolism of the androgen steroid hormones and inhibitors of this enzyme represent a new pharmacological treatment for several androgen dependent diseases. We developed a radiosubstrate in vitro incubation method for the determination of 5alpha-reductase type 1 activity using rat liver microsomes as an enzyme source. With this method we have studied the inhibiting activity of novel (5' S )-17beta-(4,5-dihydrooxazol-5-yl)androst-5-en-3-one compounds containing various derivatized phenyl substituents coupled to the exo -heterocyclic moiety. Tests revealed moderate inhibitory actions compared to finasteride, nevertheless, results provide i... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890710 Sun, 22 Aug 2010 18:45:33 +0100 3890710 http://www.medworm.com/index.php?rid=3890709&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724275%26dopt%3DAbstract In conclusion, as a result of water deficit during leaf rolling antioxidant enzymes are induced to scavenging of ROS produced in symplast and apoplast. PMID: 20724275 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890709 Sun, 22 Aug 2010 18:45:30 +0100 3890709 http://www.medworm.com/index.php?rid=3890708&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724276%26dopt%3DAbstract Authors: Labidi N, Ammari M, Mssedi D, Benzerti M, Snoussi S, Abdelly C Suaeda fruticosa is a perennial "includer" halophyte devoid of glands or trichomes with a strong ability of accumulating and sequestrating Na + and Cl - . We were interested in determining whether leaf cuticle salt excretion could be involved as a further mechanism in salt response of this species after long-term treatment with high salinity levels. Seedlings had been treated for three months with seawater (SW) diluted with tap water (0, 25, 50 and 75% SW). Leaf scanning electron microscopy revealed a convex adaxial side sculpture and a higher accumulation of saline crystals at the lamina margin, with a large variability on repartition and size between treatments. No salt gland or salt bladder was found. Threedimen... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890708 Sun, 22 Aug 2010 18:45:26 +0100 3890708 http://www.medworm.com/index.php?rid=3890707&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724277%26dopt%3DAbstract Authors: Sivakumar P, Law YS, Ho CL, Harikrishna JA An efficient in vitro plant regeneration system was established for elite, recalcitrant Malaysian indica rice, Oryza sativa L. CV. MR 219 using mature seeds as explant on Murashige and Skoog and Chu N6 media containing 2,4-dichlorophenoxy acetic acid and kinetin either alone or in different combinations. L-proline, casein hydrolysate and L-glutamine were added to callus induction media for enhancement of embryogenic callus induction. The highest frequency of friable callus induction (84%) was observed in N6 medium containing 2.5 mg l -1 2,4-dichlorophenoxy acetic acid, 0.2 mg l -1 kinetin, 2.5 mg l -1 L-proline, 300 mg l -1 casein hydrolysate, 20 mg l -1 L-glutamine and 30 g l -1 sucrose under culture in continuous lighting conditions... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890707 Sun, 22 Aug 2010 18:45:22 +0100 3890707 http://www.medworm.com/index.php?rid=3890706&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724278%26dopt%3DAbstract Authors: Kiss Z, Dobránszki J, Hudák I, Birkó Z, Vargha G, Biró S The Gram-positive soil-borne streptomycetes exhibit a complex life cycle that is controlled by extracellular regulatory molecules. One interesting autoregulator is the protein factor C, originally isolated from the culture fluid of S. albidoflavus 45H. Southern hybridizations and database searches revealed that although homologues of factor C are not present in most Streptomyces strains, an exception is the plant pathogenic S. scabies , which causes common scab disease on potato. In S. scabies and related strains pathogenicity involves a large pathogenicity island that carries thaxtomin biosynthetic genes, the nec1 necrogenic factor and other putative virulence genes. Extracellular enzymes, including extracel... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890706 Sun, 22 Aug 2010 18:45:18 +0100 3890706 http://www.medworm.com/index.php?rid=3890705&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724279%26dopt%3DAbstract Authors: Comlekcioglu U, Ozkose E, Yazdic FC, Akyol I, Ekinci MS Extracellular and cell-associated enzyme preparations were obtained from ruminal anaerobic fungi Orpinomyces sp. GMLF5 grown in culture containing microcrystalline cellulose (avicel) as sole energy source and degradation capacities of the preparations towards several polysaccharides and glycosides were studied. Fungus showed substantial increases in xylanase, carboxymethyl cellulase (CMCase), lichenase, amylase, beta-xylosidase, beta-glucosidase and alpha-L-arabinofuranosidase activities between 72 and 168 hours. High amounts of cell associated beta-xylosidase were noted in 4 and 5 days old cultures. Optimum temperature and pH of the polysaccharidases were found at 50 degrees C and 6.0-6.5, respectively. Xylanase was foun... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890705 Sun, 22 Aug 2010 18:45:14 +0100 3890705 http://www.medworm.com/index.php?rid=3890704&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724280%26dopt%3DAbstract Authors: Waśko A, Kordowska-Wiater M, Podleśny M, Polak-Berecka M, Targoński Z, Kubik-Komar A The central composite design was developed to search for an optimal medium for the growth of Lactobacillus rhamnosus OXY. The effect of various media components, such as carbon sources, simple and complex nitrogen sources, mineral agents, and growth factors (vitamins B, amino acids) was examined. The first-order model based on Plackett-Burman design showed that glucose, sodium pyruvate, meat extract and mineral salts significantly influenced the growth of the examined bacteria. The second-order polynomial regression confirmed that maximum biomass production could be achieved by the combination of glucose (12.38 g/l), sodium pyruvate (3.15 g/l), meat extract (4.08 g/l), potassium phosp... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890704 Sun, 22 Aug 2010 18:45:10 +0100 3890704 http://www.medworm.com/index.php?rid=3890703&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20724281%26dopt%3DAbstract Authors: Galgóczy L, Lukács G, Nyilasi I, Papp T, Vágvölgyi C The in vitro antifungal activity of different statins and the combinations of the two most effective ones (fluvastatin and rosuvastatin) with amphotericin B were investigated in this study on 6 fungal isolates representing 4 clinically important genera, namely Absidia, Rhizomucor, Rhizopus and Syncephalastrum . The antifungal effects of statins revealed substantial differences. The synthetic statins proved to be more effective than the fungal metabolites. All investigated strains proved to be sensitive to fluvastatin. Fluvastatin and rosuvastatin acted synergistically and additively with amphotericin B in inhibiting the fungal growth in clinically available concentration ranges. Results suggest that statins combi... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3890703 Sun, 22 Aug 2010 18:45:06 +0100 3890703 http://www.medworm.com/index.php?rid=3633702&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519167%26dopt%3DAbstract Authors: Li N, Yu ZL, Wang L, Zheng YT, Jia JX, Wang Q, Zhu MJ, Liu XL, Xia X, Li WJ The aim of this study was to investigate the effects of maternal lead exposure on the learning and memory ability and expression of tau protein phosphorylation (P-tau) and beta amyloid protein (Abeta) in hippocampus of mice offspring. Pb exposure initiated from beginning of gestation to weaning. Pb acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.1%, 0.5% and 1% groups. On the 21 th of postnatal day, the learning and memory ability of the mouse pups was tested by Water Maze test and the Pb levels in blood and hippocampus of the offspring were also determined. The expression of P-tau and Abeta in hippocampus was measured by immunohistochemi... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633702 Mon, 31 May 2010 23:00:00 +0100 3633702 http://www.medworm.com/index.php?rid=3633701&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519168%26dopt%3DAbstract Authors: Calderón GD, Esquivel GJ, García EH, Osnaya NB, Juárez Olguín H Our aim was to evaluate the effects of marijuana (Mar) and diazepam (Dz) on lipid peroxidation (TBARS), Na + , K + ATP ase activity, levels of glutathione (GSH) and 5-hydroxytryptophan (5-HTP). Male Wistar rats were given a single dose per group: extract of Mar (100 muL/kg), Dz (5 mg/kg), Mar plus Dz, and NaCl for control. Sixty mins after treatment, animals were sacrificed, and their brains extracted and homogenised to measure GSH, TBARS and 5-HTP levels. Na + , K + ATP ase and total ATP ase activities. GSH and TBARS did not show differences respect to controls. Na + , K + ATP ase activity was similar as well. However, groups treated with Mar, total ATPase activity decreased significantly (p < 0.05... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633701 Mon, 31 May 2010 23:00:00 +0100 3633701 http://www.medworm.com/index.php?rid=3633700&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519169%26dopt%3DAbstract Authors: Amara IB, Bouaziz H, Guermazi F, Zeghal N The present study was undertaken to assess the effect of selenium (Se) on hypothyroidism induced by methimazole (MMI) in lactating rats and their pups. Rats were randomly divided into four groups of six each: group I served as a negative control which received standard diet; group II received orally MMI (250 mg L -1 ); group II received both MMI (250 mg L -1 , orally) and Se (0.5 mg/kg of diet); group IV served as a positive control and received Se (0.5 mg Na 2 SeO 3 /kg of diet). Treatments were started from the 14th day of pregnancy until postnatal day 14. In the MMI-exposed group, the body weight of 14-day-old pups diminished compared to controls; besides, a hypertrophy of the thyroid glands was observed. Co-administration of Se thr... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633700 Mon, 31 May 2010 23:00:00 +0100 3633700 http://www.medworm.com/index.php?rid=3633699&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519170%26dopt%3DAbstract In this study the effect of ELFEMF on the number of apoptotic cells of K562 human leukemia cell line induced or not with oxidative stress and the correlation with heat-shock protein 70 (hsp70) levels was investigated. One sample was treated with H 2 O 2 while the other was left untreated. ELF-EMF (1 mT, 50 Hz) was applied for 3 hours. ELF-EMF alone caused a decrease in the number of apoptotic cells and a slight increase in viability. However, it increased the number of apoptotic cells. In cells treated with H 2 O 2 . hsp70 and reactive oxygen species (ROS) levels were increased by ELF-EMF. These results show that the effect of ELF-EMF on biological systems depends on the status of the cell: while in cells not exposed to oxidative stress it is able to decrease the number of apoptotic cells ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633699 Mon, 31 May 2010 23:00:00 +0100 3633699 http://www.medworm.com/index.php?rid=3633698&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519171%26dopt%3DAbstract In this study we evaluated the effects of long-term therapy with L-arg in the MF of mdx mice. mdx mice (6 months old) were treated with L-arg in drinking water. Control mdx mice received water only. After 15 months of treatment, hearts were stained with Masson's trichrome for analysis of MF and with hematoxilyn and eosin for analysis of inflammation and cardiomyocyte damage. We observed that MF was not affected (29.5 +/- 2.5% of MF area for control vs 31.4 +/- 2% for L-arginine-treated animals; P > 0.05). The density of inflammatory cells was reduced (169 +/- 12 cells/mm 2 in control vs 102 +/- 9 cells/mm 2 in L-arg-treated; P < 0.05). The present study shows that long-term administration of L-arg is not effective in retarding MF in mdx dystrophinopathy. PMID: 20519171 [PubMed - ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633698 Mon, 31 May 2010 23:00:00 +0100 3633698 http://www.medworm.com/index.php?rid=3633697&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519172%26dopt%3DAbstract Authors: Mahesh A, Jeyachandran R, Cindrella L, Thangadurai D, Veerapur VP, Muralidhara Rao D The hepatocurative potential of ethanolic extract (ETO) and sesquiterpene lactones enriched fraction (SL) of Taraxacum officinale roots was evaluated against carbon tetrachloride (CCl 4 ) induced hepatotoxicity in mice. The diagnostic markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin contents were significantly elevated, whereas significant reduction in the level of reduced glutathione (GSH) and enhanced hepatic lipid peroxidation, liver weight and liver protein were observed in CCl 4 induced hepatotoxicity in mice. Post-treatment with ETO and SL significantly protected the hepatotoxicity as evident from the lower l... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633697 Mon, 31 May 2010 23:00:00 +0100 3633697 http://www.medworm.com/index.php?rid=3633696&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519173%26dopt%3DAbstract Authors: Bouazizi H, Jouili H, Geitmann A, Ferjani E The effect of copper excess (CuSO 4 ) on lipid peroxidation, H 2 O 2 content, and antioxidative enzyme activities was studied in primary leaves of bean seedlings. Fourteen-day-old bean seedlings were cultured in a nutrient solution containing Cu 2+ at various concentrations (50 and 75 muM) for 3 days. Excess of copper significantly increased malondialdehyde content and endogenous H 2 O 2 . This radical accumulated in the intercellular spaces of palisade mesophyll cells. In addition, cupric stress induced changes in antioxidant enzyme activities. GPX (guaiacol peroxidase, EC 1.11.1.7) activity was decreased in 50 muM Cu-stressed leaves whereas 75 muM of CuSOP 4 resulted in an increase of enzyme activity. On the contrary, CAT (catalase... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633696 Mon, 31 May 2010 23:00:00 +0100 3633696 http://www.medworm.com/index.php?rid=3633695&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519174%26dopt%3DAbstract Authors: Karray-Bouraoui N, Attia H, Maghzaoui M, Msilini N, Rabhi M, Lachaâl M Physiological responses of Arabidopsis thaliana to the interaction of iron deficiency and nitrogen form were studied using plants grown in hydroponics. Thirty-three-day-old seedlings were submitted to four treatments for 7 days: NO 3 + 5 muM Fe; NO 3 + 0.1 muM Fe; NH 4 + 5 muM Fe and NH 4 + 0.1 muM Fe. Leaf growth and chlorophyll content were highest in NO 3 -fed, Fe sufficient plants, but were strongly diminished by Fe deficiency under nitric nutrition, and by ammoniacal nutrition independently of Fe regime. However, the leaves of NH 4 -fed plants presented a higher Fe content than those of Fe sufficient, NO 3 -fed plants. Thus, leaf chlorosis of NH 4 -fed in plant did not depend on Fe availability, and... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633695 Mon, 31 May 2010 23:00:00 +0100 3633695 http://www.medworm.com/index.php?rid=3633694&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519175%26dopt%3DAbstract Authors: Taski-Ajdukovic K, Nagl N, Miladinovic D Successful application of somatic hybridization in sunflower breeding is limited by low regeneration percentage from fusion products. The effect of pre-treatment media composition on shoot regeneration from electrofusion products of wild and cultivated sunflower was investigated in this paper, with the aim to determine if there is room for improvement of the existing regeneration protocols in order to make them less genotype-dependent. Protoplasts isolated from hypocotyls of two inbred lines of cultivated sunflower were electrofused with mesophyll protoplasts of H. mollis and H. maximiliani . The regeneration was induced only on calluses that were grown on the media with low auxin and high cytokinin contents during the development of ca... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633694 Mon, 31 May 2010 23:00:00 +0100 3633694 http://www.medworm.com/index.php?rid=3633693&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519176%26dopt%3DAbstract Authors: Chmielarz P In order to evaluate the feasibility of cryopreservation of Wych elm ( Ulmus glabra Huds.) seeds, we evaluated the seeds sensitivity to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; -196 degrees C). We also determined the critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or up to two years in LN. Germination tests revealed no critical WC for seeds to 0.03 g H 2 O g -1 dry mass, g g -1 . Seeds tolerated freezing in LN within safe ranges of WC 0.03-0.21 g g -1 (nuts). Seeds desiccated to the safe WC and stored in LN for two years had similar germination as seeds stored at -3 degrees C for two years. Therefore, long-term cryopreservatio... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633693 Mon, 31 May 2010 23:00:00 +0100 3633693 http://www.medworm.com/index.php?rid=3633692&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20519177%26dopt%3DAbstract Authors: Hudák I, Dobránszki J, Sárdi E, Hevesi M Endogenous carbohydrate (fructose, glucose and sucrose) fractions were measured in calli of potato genotypes with different field tolerance to drought. Under in vitro stress conditions induced by 0.8 M mannitol, sucrose level of calli increased extremely in medium-tolerant (by 424.5%) and sensitive (by 302.7%) genotypes whilst the rate of increase was 12-18-times lower in the drought tolerant variety. Results suggest the applicability of sucrose as biochemical marker for distinguish drought tolerant genotypes from great population in callus culture. PMID: 20519177 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3633692 Mon, 31 May 2010 23:00:00 +0100 3633692 http://www.medworm.com/index.php?rid=3328084&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194094%26dopt%3DAbstract This study was performed to investigate the effects of L-thyroxine treatment on plasma vasopressin (AVP) levels in rats with hypothyroidism induced by propylthiouracil (PTU). Animals were separated into three groups each having 6 rats: control, PTU, PTU+L-thyroxine groups. Then, the groups were further divided into 3 sub-groups including 6 rats (a; basal, b; hypertonic stimulated and c; hypovolemic stimulated). At the end of the experiments all rats were decapitated in order to obtain plasma samples for analysis in terms of Hct, osmolality, TT 3 , TT 4 and vasopressin. Haematocrit (Hct) levels were the highest in hypovolemic stimulated sub-group (P < 0.001). Osmolality levels were higher in hypertonic stimulated sub-groups (P < 0.001). Total T 3 and T 4 values were the lowest in the ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328084 Mon, 01 Mar 2010 00:00:00 +0100 3328084 http://www.medworm.com/index.php?rid=3328083&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194095%26dopt%3DAbstract Authors: Said Ali K, Ferencz A, Nemcsók J, Hermesz E Heat shock proteins (HSPs) and metallothioneins (MTs) play important roles in protection against environmental stressors. The present study analyzes and compares the regulation of heat shock ( hsp70, hsc70-1 and hsp90alpha ) and metallothionein (MT-1 and MT-2) genes in the heart of common carp, in response to elevated temperature, cold shock and exposure to several heavy metal ions (As 3+ , Cd 2+ and Cu 2+ ), in whole-animal experiments. Among these metals, arsenate proved to be the most potent inducer of the examined stress genes; the hsp90alpha and MT-1 mRNA levels were elevated 11- and 10-fold, respectively, after a 24-h exposure. In contrast, Cd 2+ at 10 mg/L had no impact on the expression of hsp90alpha , and the MT genes a... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328083 Mon, 01 Mar 2010 00:00:00 +0100 3328083 http://www.medworm.com/index.php?rid=3328082&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194096%26dopt%3DAbstract Authors: Hegyi A, Urbányi B, Kovács M, Lefler KK, Gál J, Hoitsy G, Horváth A The transportation of rainbow trout in the presence of the anesthetic clove oil was investigated. Before the transportation tests, an acute experiment was conducted to verify that removal of the fish from the water for one minute does not significantly increase the glucose or cortisol concentration of the blood plasma. In the main experiment two different transportation conditions were compared: transport in water only and in water with anesthetic. During transportation without addition of clove oil, blood plasma glucose and cortisol concentrations changed significantly. The concentration of glucose increased from 4.92 mmol/L prior to transportation to 6.16 mmol/L and values similar to the ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328082 Mon, 01 Mar 2010 00:00:00 +0100 3328082 http://www.medworm.com/index.php?rid=3328081&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194097%26dopt%3DAbstract Authors: Eissa FI, Zidan NA The renal- and hepato-toxicity induced by abamectin pesticide (Vertimec) and a commercial form of a bio-insecticide Bacillus thuringiensis (Agerin) in male albino rats were evaluated. Blood picture and blood glucose level were investigated. Male albino rats were administered dietary doses each equivalent to 1/10 or 1/100 of the LD 50 values of each toxicant for 30 consecutive days. Abamectin was found to pose risks of renal- and hepato-toxicity in rats, since the biochemical parameters of liver function (i.e. aspartate aminotransferase activity, alanine aminotransferase activity, acid phosphatase activity, albumin, and total protein levels) and kidney function (uric acid and creatinine concentration) were severely affected. These effects were verified by his... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328081 Mon, 01 Mar 2010 00:00:00 +0100 3328081 http://www.medworm.com/index.php?rid=3328080&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194098%26dopt%3DAbstract Authors: Szentmihályi K, Gere A, Szöllösi-Varga I, Blázovics A, Jasztrab S, Ladó K, Then M Plant drug mixtures are widely used in the adjuvant therapy of type 2 diabetes mellitus for the prevention of complications. The drug mixtures generally contribute to the efficiency of the therapy and may also reduce undesirable side effects. Two herbal extracts (lyophilized aqueous extracts of plant drug mixtures 1: Myrtilli folium, Phaseoli fructus sine seminibus and 2: Myrtilli folium, Phaseoli fructus sine seminibus, Salviae folium ) were investigated in in vitro rat models. The content of bioactive constituents (polyphenol, flavonoid and vitamin C) in plant drug mixtures and lyophilized samples was evaluated. The antioxidant activity of lyophilized extracts was deter... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328080 Mon, 01 Mar 2010 00:00:00 +0100 3328080 http://www.medworm.com/index.php?rid=3328079&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194099%26dopt%3DAbstract Authors: Sun L, Yu F, Xu Z, Zeng X, Ferreri M, Han B Objective of this study was to assess the quantification of osteocalcin (OCN) expression by ovine osteoblasts cultured with different concentrations of sodium fluoride (F) and sodium selenite (Se) to evaluate the interaction of these agents on OCN expression in vitro . We wanted to demonstrate a possible protective effect of selenium on the toxic effect of fluoride. Osteoblasts were isolated by complete trypsin and collagenase digestion from ovine calvarial bone and cultured in DMEM supplemented with 15% FBS at 37 degrees C in a humidified 5% CO 2 incubator. Identified osteoblasts were divided into one control group (C) and eight experimental groups, which were exposed to different concentrations of sodium fluoride (F; 0, 0.5, 1 mM) ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328079 Mon, 01 Mar 2010 00:00:00 +0100 3328079 http://www.medworm.com/index.php?rid=3328078&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194100%26dopt%3DAbstract We present novel data for ABH antigen reactivity in reproductive organs of vertebrates which have not been studied so far. Our results suggest that HBGA are evolutionary stable structures, most possibly related to vitellogenesis in oocytes with high yolk content. The tendency observed in A and B antigen expression is mostly associated with the processes of gamete differentiation and vitellogenesis, rather than with the evolutionary development of vertebrate species. PMID: 20194100 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328078 Mon, 01 Mar 2010 00:00:00 +0100 3328078 http://www.medworm.com/index.php?rid=3328077&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194101%26dopt%3DAbstract Authors: Sharifi G, Ebrahimzadeh H Among the different concentrations of Thidiazuron (TDZ) and between the two media Gamborg (B5) and Murashige and Skoog (MS), the highest frequency of shoot formation could be seen in the MS medium with TDZ concentration of 4.54 muM. Among the different concentrations of Naphtalene acetic acid (NAA) and Benzyl adenine (BA) in the two aforementioned media, the maximum proliferation and rooting of saffron shoots were obtained in a B5 medium containing 2.22 muM NAA and 2.68 muM BA. Peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), esterase (EST) and polyphenoloxidase (PPO) measurements proved that all the enzymes had a similar pattern of changes, according to which their concentrations increased in the first stages of development and then decr... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328077 Mon, 01 Mar 2010 00:00:00 +0100 3328077 http://www.medworm.com/index.php?rid=3328076&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194102%26dopt%3DAbstract Authors: Borowiak K, Zbierska J, Drapikowska M Tropospheric ozone causes visible injuries in selected plants. Some plant species have been recognized as resistant and sensitive to ozone. Differences in the response to ozone of two kinds of plants are interesting because of practical implications. Resistant cultivars (without visible symptoms) will be more valuable for agriculture, forestry and horticulture. It is, however, necessary to find out the reason of the occurrence of the visible symptoms. The presented studies focused on selected morphological and anatomical differences between well-known ozone-sensitive (Bel W3) and -resistant (Bel B) cultivars of tobacco plants. Daily growth of the sensitive cultivar was higher than that of the resistant one. This tendency was also valid for... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328076 Mon, 01 Mar 2010 00:00:00 +0100 3328076 http://www.medworm.com/index.php?rid=3328075&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194103%26dopt%3DAbstract Authors: Takó M, Farkas E, Lung S, Krisch J, Vágvölgyi C, Papp T Extracellular beta-glucosidase activity of 94 strains, representing 24 species of the genera Gilbertella, Mucor, Rhizomucor , and Rhizopus was evaluated in submerged culture and under solid state fermentation on wheat bran. Gilbertella persicaria G1 isolate showed the highest activity (70.9 U ml -1 ) followed by other Gilbertella (58.6-59.0 U ml -1 ) and Rhizomucor miehei isolates (29.2-42.0 U ml -1 ). Optimum temperature for enzyme production was 25 degrees C for Gilbertella and Mucor , and 30 degrees C for Rhizomucor and Rhizopus strains. Enzymes of R. miehei strains proved to be thermotolerant preserving up to 92.8% residual activity after heating to 75 degrees C in the presence of cellobiose substrate. ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328075 Mon, 01 Mar 2010 00:00:00 +0100 3328075 http://www.medworm.com/index.php?rid=3328074&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194104%26dopt%3DAbstract Importance of acidic phosphatase activity in P supply and Gonyostomum semen Ehrenbergh (Raphidophyta) occurrence in a Hungarian peat bog, Keleméri Kis-Mohos (Ne Hungary). Acta Biol Hung. 2010 Mar;61(1):111-21 Authors: Grigorszky I, Dévai G, Kiss KT, Tóthmérész B, Gligora M, Plenkovic-Moraj A, Kraj K, Béres VB, Schnitchen C, Borics G, Nagy AS Phosphatase enzymes are capable of releasing phosphate through cleavage of phosphoester bonds. The seasonal importance of this process was examined by using a model substrate paranitrophenylphosphate and the Michaelis-Menten equation to estimate the release rate of PO 4 -P from phosphomonoesters. The seasonal occurrence of phosphomonoesters and acid phosphatase activity was used to estimate the velocity of phos... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328074 Mon, 01 Mar 2010 00:00:00 +0100 3328074 http://www.medworm.com/index.php?rid=3105499&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015826%26dopt%3DAbstract Authors: Jójárt J, Jójárt I, Boda K, Gálfi M, Mihály A, B-Baldauf Z, Vecsernyés M We investigated the distribution of oxytocin in rat spinal cord using immunocytochemistry and radioimmunoassay (RIA). Each segment of the spinal cord from cervical to coccygeal contained oxytocin-immunoreactive fibers. The Rexed laminae I and II of the dorsal horn showed moderate to intense immunoreactivity. A dense network was found around the central canal where some fibers apposed the ependyma. The autonomic centers of the spinal cord at the thoracolumbar and sacral segments were heavily innervated. Few fibers were found around the motoneurons. In the white matter, the immunoreactivity was localized mainly in the dorsal part of the lateral funiculus, in the pars funic... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105499 Tue, 01 Dec 2009 00:00:00 +0100 3105499 http://www.medworm.com/index.php?rid=3105498&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015827%26dopt%3DAbstract Authors: Bódi N, Battonyai I, Talapka P, Fekete E, Bagyánszki M Nitrergic myenteric neurons are especially susceptible to the development of neuropathy in functional gastrointestinal disorders. Investigations of the similarities and dissimilarities in the organization of nitrergic neurons in the various mammalian species are therefore important in an effort to determine the extent to which the results obtained in different animal models can be generalized. In the present work, the density and the spatial organization of the nitrergic neurons in the myenteric plexus of the duodenum were investigated in 7 mammalian species. After nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry, the Plexus Pattern Analysis software (PPAs) was applied to count the ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105498 Tue, 01 Dec 2009 00:00:00 +0100 3105498 http://www.medworm.com/index.php?rid=3105497&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015828%26dopt%3DAbstract Authors: Lukács A, Szabó A, Papp A, Vezér T 3-nitropropionic acid (3-NP), a substance used for modelling Huntington's disease, was given to male Wistar rats in a single 20 mg/kg b.w. dose, and the resulting behavioral alterations in spontaneous locomotor activity were measured after 30 minutes. To detect the involvement of neurotransmitter systems in this immediate effect, the NMDA antagonist MK-801 (0.8 mg/kg); as well as an agonist, quinpirole (QP, 5 mg/kg) and an antagonist, sulpiride (SP, 80 mg/kg) of the dopamine D2 receptors, were given before 3-NP to separate groups of rats. Controls were given saline. All substances were injected ip. 3-NP decreased the rats' locomotor, especially vertical, activity, whereas local activity was increased. Based on the further chang... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105497 Tue, 01 Dec 2009 00:00:00 +0100 3105497 http://www.medworm.com/index.php?rid=3105496&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015829%26dopt%3DAbstract Authors: Krsková L, Vrabcová M, Talarovicová A, Zeman M The renin-angiotensin system (RAS) plays an important role in the development of hypertension and has serious consequences on behaviour. The aim of our study was to investigate the effect of hypertension, induced by up-regulated RAS, on the exploration, anxiety-related behaviour and object recognition in laboratory rats. In the experiment, 12 weeks old normotensive Sprague-Dawley (SD) and hypertensive TGR(mREN2)27 (TGR) male rats with up-regulated RAS were used. In the open-field test, the TGR rats were less active in ambulating, rearing and sniffing and more active in self-grooming and urinating than SD ones. In the elevated plus-maze test, the TGR rats showed lower frequency of total arm entries, closed arm entrie... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105496 Tue, 01 Dec 2009 00:00:00 +0100 3105496 http://www.medworm.com/index.php?rid=3105495&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015830%26dopt%3DAbstract Authors: Nadia BH, Wided K, Kheira B, Hassiba R, Lamia B, Rhouati S, Alyane M, Zellagui A, Lahouel M This paper reports an investigation of the ability of propolis extract (a resinous substance collected by honeybees from various plant sources) to restore the collapse of mitochondrial membrane potential induced by ferulenol, a sesquiterpene prenylated coumarin derivative isolated from the plant Ferula vesceritensis . We show that ferulenol was able to induce the permeability transition pore (PTP) opening. This effect is caused by the interaction of the compound with the mitochondrial respiratory chain, more particularly by the fall of membrane potential and the inhibition of complex II. We have previously demonstrated that this inhibition results from a limitation of electron transfers... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105495 Tue, 01 Dec 2009 00:00:00 +0100 3105495 http://www.medworm.com/index.php?rid=3105494&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015831%26dopt%3DAbstract This study was undertaken to examine the effect of melatonin on leptin production using male C57BL/6 adult mice treated with or without daily melatonin supplements (10 mug/mL) in drinking water for 1 month. In addition, in vitro experiments using adipose tissue fragments derived from epididymal fat pads of adult mice incubated with or without melatonin (1 nM) administration were also conducted. The results showed that melatonin-supplemented mice had significantly higher plasma leptin levels than control mice. However, melatonin incubation did not cause any marked changes in the amount of leptin secreted from adipose tissue fragments. Our findings from this study indicate that melatonin does not affect leptin secretion via mouse adipose tissue. Nevertheless, melatonin could still influence ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105494 Tue, 01 Dec 2009 00:00:00 +0100 3105494 http://www.medworm.com/index.php?rid=3105493&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015832%26dopt%3DAbstract In this study, in order to elucidate the toxic nuclear effects of dichlorvos, freshly isolated human peripheral blood lymphocytes were incubated with 5, 10, 20, 40, 80 and 100 mug/mL of dichlorvos. According to the results, dichlorvos induced micronuclei, decreased the mitotic and replication indexes. It is a genotoxic product causing chromosomal damage (an increase in micronucleus) and cell death (decrease in mitotic and replication indexes). PMID: 20015832 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105493 Tue, 01 Dec 2009 00:00:00 +0100 3105493 http://www.medworm.com/index.php?rid=3105492&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015833%26dopt%3DAbstract Authors: Terzi R, Saruhan N, Sağlam A, Nar H, Kadioğlu A We studied the changes in antioxidant system and chlorophyll fluorescence parameters in post-stress emerging Ctenanthe setosa (Rosc.) Eichler (Marantaceae) plants (PSE plants) having reduced leaf area under drought stress causing leaf rolling and re-watering. PSE plants were compared to primary stressed plants (PS) in previous studies. The parameters were measured at different visual leaf rolling scores from 1 to 4 (1 is unrolled, 4 is tightly rolled and the others is intermediate form). Water potentials and stomatal conductance of leaves were gradually decreased during leaf rolling. Similarly, maximum quantum efficiency of open PS II center and quantum yield of PS II decreased during the rolling period. Non-photochem... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105492 Tue, 01 Dec 2009 00:00:00 +0100 3105492 http://www.medworm.com/index.php?rid=3105491&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015834%26dopt%3DAbstract Authors: Atia A, Debez A, Rabhi M, Smaoui A, Abdelly C Interaction of salinity, nitrate, light, and seed weight on the germination of Crithmum maritimum was investigated. Seeds of three size categories were germinated at 0-200 mM NaCl with either 0, 5 or 20 mM KNO 3 . Experiments were done under darkness, white light, or red light. Regardless of seed weight, germination was maximal in distilled water. Under salinity, the smallest seeds showed the highest germination percentage. Salt impact was amplified by darkness, but was mitigated by nitrate supply, red light and their combination. At the same PPFD, germination of T2 seeds was higher, when exposed to red light than under white light, suggesting that germination was more influenced by the light type than by the PPFD. As a whole, not ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105491 Tue, 01 Dec 2009 00:00:00 +0100 3105491 http://www.medworm.com/index.php?rid=3105490&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015835%26dopt%3DAbstract Authors: Nasim SA, Mujib A, Rashmi K, Samar F, Junaid A, Mahmooduzzafar Direct somatic embryo formation (without intervening callus) from garlic clove basal tissue was induced in which the influence of plant growth regulators (PGRs) on various explants was examined. Medium added with 2.0 mg/l 6-benzylaminopurine (BAP) and 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) were the most effective PGR combination for somatic embryo induction. It induced embryos directly in 85.5% of the basal clove explant. Callus induction was also obtained from other parts of explant and 2.0 mg/l 2,4-D induced callusing in 86.5% of the inoculated explants. Protein, amino acid and alliin content were measured in callus and in embryos. Somatic embryos had more soluble protein and free amino acid compared to... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105490 Tue, 01 Dec 2009 00:00:00 +0100 3105490 http://www.medworm.com/index.php?rid=2735096&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700383%26dopt%3DAbstract This study suggests the probable interaction between Ang II and opioid system in the VTA. PMID: 19700383 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735096 Thu, 27 Aug 2009 04:00:28 +0100 2735096 http://www.medworm.com/index.php?rid=2735095&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700384%26dopt%3DAbstract Authors: Cherici Camargo IC, Barreiros De Souza R, Fátima Paccola Mesquita S, Chuffa LG, Frei F The study was conducted to analyze the histology of the ovaries of adults rats treated with steroids, and submitted or not to physical effort. The control group consisted of females submitted to physical effort and sedentary females, both of which received a physiological solution of 0.9% saline. Treated females, sedentary or not, received 6 mg/kg of body weight of nandrolone decanoate. The steroid and physiological solution were administered intraperitoneally, with a single injection per week for 4 consecutive weeks. The applied physical effort was swimming (20 minutes daily, 5 days/week, for the 4 weeks of treatment). Serial sections (5 mum) of ovaries were prepared for histological e... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735095 Thu, 27 Aug 2009 04:00:26 +0100 2735095 http://www.medworm.com/index.php?rid=2735094&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700385%26dopt%3DAbstract Authors: Lukasik I, Goławska S, Leszczyński B Effects of some dietary o -dihydroxyphenols on biochemical markers of oxidative stress within tissues of Sitobion avenae (Fabricius), (Homoptera, Aphididae) and Rhopalosiphum padi (Linnaeus), (Homoptera, Aphididae) has been studied. Among the studied aphid morphs the highest concentration of total thiols, hydrogen peroxide and lipid peroxidation products (TBARS) was noted for winged adults (alatae). Higher content of H 2 O 2 was observed within tissues of the oligophagous species R. padi while the monophagous species S. avenae had higher TBARS and total thiols concentration. Aphids exposed the dietary pro-oxidative o -dihydroxyphenols (quercetin, caffeic acid, chlorogenic acid) demonstrated significantly lower concentration of t... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735094 Thu, 27 Aug 2009 04:00:22 +0100 2735094 http://www.medworm.com/index.php?rid=2735093&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700386%26dopt%3DAbstract Authors: Nciri R, Allagui MS, Vincent C, Croute F, Elfeki A Lithium salts are efficiently used for treatment of psychiatric disorders. However, prolonged treatment frequently involves adverse side-effects. In the present work, effects of lithium carbonate administration on some biochemical parameters were studied in male mice. Lithium carbonate (20, 40 or 80 mg/kg body weight, corresponding to 3.77, 7.54 or 15.08 mg Li element/kg body weight, respectively) was injected daily for 14 or 28 days. The following parameters were recorded: water consumption, body weight, lithium and testosterone serum concentrations, activities of catalase (CAT), superoxide-dismutase (SOD) and glutathione-peroxidase (GPX) and level of lipid peroxidation (expressed as TBARS) in liver was performed. Lithium tre... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735093 Thu, 27 Aug 2009 04:00:20 +0100 2735093 http://www.medworm.com/index.php?rid=2735092&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700387%26dopt%3DAbstract Authors: Chen Q, Cao M, Xiang WL, Sun Q, Zhang J, Hou RT, Yan ZY, Yang ZR, Liu J, Zhao J The forward and reverse cDNA subtractive libraries before and after the toxic effect of alpha-amanitin were constructed by suppression subtractive hybridization and randomly selected clones from each subtractive library were screened by PCR and dot blot hybridization. A total of 85 genes with altered expression were finally identified, with 41 genes from the forward library and 44 from the reverse library. Subsequently, the antagonistic effects of candidate traditional Chinese medicines were evaluated based on the genetic transcription levels of the genes with significant altered expression, including Catnbeta, Flt3-L, IL-7r and Rpo2-4. The results indicated that Silybum marianum (L.) Gaert and Gan... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735092 Thu, 27 Aug 2009 04:00:17 +0100 2735092 http://www.medworm.com/index.php?rid=2735091&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700388%26dopt%3DAbstract Authors: Refaey M, Kováts N, Kárpáti A, Thury P Whole effluent toxicity is most often considered as a static parameter. However, toxicity might change as degradation processes, especially biodegradation goes by and intermediate products appear. These intermediates can even be more toxic than the original effluent was, posing higher risk to the ecosystem of the recipient water body. In our test series it was assessed how toxicity of a municipal wastewater sample changes during biodegradation taking into consideration different temperature regimes (10, 20 and 30 degrees C). Results proved our null hypothesis: after the high initial toxicity of the fresh effluent sample toxicity did show a further increase. Biodegradation resulted in toxicity reduction only after an approx.... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735091 Thu, 27 Aug 2009 04:00:15 +0100 2735091 http://www.medworm.com/index.php?rid=2735090&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700389%26dopt%3DAbstract In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4-6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA - 6-benzylaminopurine; NAA - alpha-naphthaleneacetic acid; TDZ - 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 muM NAA + 8.87 muM BA and 2.68 muM NAA + 17.74 muM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 muM NAA + 4.44 muM BA produced somatic embryos at a frequency of 38%. PMID... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735090 Thu, 27 Aug 2009 04:00:12 +0100 2735090 http://www.medworm.com/index.php?rid=2735089&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700390%26dopt%3DAbstract In this study, induction of regenerable callus from mature embryos in eight Turkish barley varieties was analysed by using different plant growth regulators (PGRs). Varying concentrations (0.5-4 mg l -1 ) of 2,4-dichlorophenoxyacetic acid (2,4-D) and dicamba (3,6-dichloro- o -anisic acid) were tested for callus induction from mature embryos. Highest percent of callus induction was observed in Bornova 92 variety (98.3%) on MS medium supplemented with 4 mg l -1 dicamba. Calli were transferred to regeneration media with 0.5 mg l -1 dicamba, 0.5 mg l -1 zeatin riboside (ZR) and 2 mg l -1 thidiazuron (TDZ). Low concentrations of dicamba induced multiple shoots during callus regeneration. When the effect of precultivation with 2,4-D or dicamba on the shoot induction were evaluated, lower concent... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735089 Thu, 27 Aug 2009 04:00:10 +0100 2735089 http://www.medworm.com/index.php?rid=2735088&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700391%26dopt%3DAbstract Authors: Tóth V, Antal K, Gyémánt G, Miskei M, Pócsi I, Emri T Coprogen production of Neurospora crassa was dependent on glucose, aspartate and iron contents as well as on initial pH of the culture media. Surplus iron and acidic pH hindered the production of coprogen as well as the transcription of the sid1 gene (NCU07117) encoding putative L-ornithine-N 5 -monooxygenase, the first enzyme in the coprogen biosynthetic pathway. High glucose (40 g/l) and aspartate (21 g/l) concentrations were beneficial for coprogen synthesis, but neither glucose nor aspartate affected the sid1 transcription. Moreover, efficient coprogen production was observed after glucose had been consumed, which suggested that N. crassa accumulated iron even in non-growing, carbon-starving cultures... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735088 Thu, 27 Aug 2009 04:00:08 +0100 2735088 http://www.medworm.com/index.php?rid=2735087&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700392%26dopt%3DAbstract Authors: Zenke P, Maróti-Agóts A, Pádár Z, Zöldág L Demand for correct and cost-effective genetic-based identification and parentage control has increasing importance in domestic animals, including dogs. In our study the applicability of a canine hyperpolymorphic microsatellite marker - which localized in the WILMS-TF (tumor factor) gene - was examined in mixed breed and purebred canine populations. The redesigned and shortened amplicons were genotyped using an allelic ladder which was constructed from sequence verified fragments. The nomenclature for allele calling based on repetition structures is suitable for international comparisons. Our study justified the potential use and efficiency of the marker D18S12 in parentage control. PMID: 19700392 [Pub... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735087 Thu, 27 Aug 2009 04:00:05 +0100 2735087 http://www.medworm.com/index.php?rid=2585335&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584023%26dopt%3DAbstract Authors: Pekcetin C, Kiray M, Ergur BU, Tugyan K, Bagriyanik HA, Erbil G, Baykara B, Camsari UM Cerebral ischemia leads to cognitive decline and neuronal damage in the hippocampus. Reactive oxygen species (ROS) play an important role in the neuronal loss after cerebral ischemia and reperfusion injury. Carnosine has both antioxidant and neuroprotective effects against ROS. In the present study, the effects of carnosine on oxidative stress, apoptotic neuronal cell death and spatial memory following transient cerebral ischemia in rats were investigated. Transient ischemia was induced by occlusion of right common carotid artery of rats for 30 min and reperfusion for 24 h or 1 week. Rats received intraperitoneal injection of 250 mg/kg carnosine or saline 30 min prior to experiment. Determin... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585335 Sun, 31 May 2009 23:00:00 +0100 2585335 http://www.medworm.com/index.php?rid=2585334&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584024%26dopt%3DAbstract Authors: Ali KS, Ferencz A, Deér AK, Nemcsók J, Hermesz E The expression pattern of two metallothionein (MT) genes in response to temperature shock and exposure to Cd 2+ was investigated in the brain of common carp ( Cyprinus carpio ), in whole-animal experiments. The changes in the levels of MT-1 and MT-2 mRNA in the olfactory lobe, midbrain and cerebellum were followed by semiquantitative RT-PCR. The inducibility of the two MT genes was brain regionand stressor-specific. Cd 2+ affected mostly the expression of MT-2, while the level of the MT-1 transcript did not change significantly in any of the brain regions examined. Moreover, the MT-2 expression was regulated spatially; MT-2 was induced significantly more strongly in the olfactory lobe than in the cerebellum or midbrain... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585334 Sun, 31 May 2009 23:00:00 +0100 2585334 http://www.medworm.com/index.php?rid=2585333&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584025%26dopt%3DAbstract Authors: Gülsoy N, Colak S This paper describes various aspects of previtellogenic oocyte growth in sharpsnout seabream, Diplodus puntazzo , is an important marine culture fish species in the Mediterranean. The ultrastructural characteristics of nuclear morphology, nuclear-cytoplasmic ratio and the starting of the follicle envelope formation were described in detail. These cells do not significantly differ from those of the other teleost species. The ultrastructural aspects provide new information on the reproductive biology of Sparidae. PMID: 19584025 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585333 Sun, 31 May 2009 23:00:00 +0100 2585333 http://www.medworm.com/index.php?rid=2585332&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584026%26dopt%3DAbstract Authors: Holecková B, Siviková K, Dianovský J Bendiocarb is a carbamate broad-spectrum insecticide used to control disease vectors such as mosquitoes and flies, as well as household and agricultural pests. Nowadays, only few papers reporting cytogenetic or possible genotoxic effect of this insecticide on mammalian cells are available. In the present study 24-hour exposure to bendiocarbamate at concentrations ranging from 20 to 160 mug/ml was used for investigation of unstable chromosomal aberrations (CA), sister chromatid exchanges (SCE) and stable chromosomal aberration induction in cultured bovine peripheral lymphocytes. The slight but no significant increase of chromatide breaks frequency was observed after the exposure of lymphocytes to 80 mug/ml of bendiocarb. At th... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585332 Sun, 31 May 2009 23:00:00 +0100 2585332 http://www.medworm.com/index.php?rid=2585331&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584027%26dopt%3DAbstract In this study Maximum Tolerated Dose (MTD) was obtained according to CSGMT protocol presented by the Environmental Mutagen Society of Japan. The positive group received mitomycin C in dose of 0.5 mg/kg. The peripheral blood samples before treatment (zero time) were considered as negative control. The MTD of Hypiron was 12 ml/kg and for Chamomilla was 10 ml/kg. Doses of MTD, 1/2 MTD and 1/4 MTD were considered for test groups. Then blood samples were prepared 48 hours after first administration of drugs and kept on precoated Acridine orange slides. The scoring of micronucleated reticulocytes were carried out per 2000 counted reticulocytes in each slide by fluorescent microscope. The results were statistically analyzed. Results of Hypiran were compared with negative control group and the P v... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585331 Sun, 31 May 2009 23:00:00 +0100 2585331 http://www.medworm.com/index.php?rid=2585330&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584028%26dopt%3DAbstract Authors: Gonchar O, Mankovskaya I In an experimental model, it was shown that repetitive periods of hypoxia/reoxygenation (H/R) [5 cycles of 5 min hypoxia (12% O 2 in N 2 ) followed by 15 min normoxia, daily for three weeks] attenuated basal and stimulated in vitro lipid peroxidation, as well as H 2 O 2 production in liver and brain mitochondria of rats exposed to acute severe hypoxia. Adaptation to moderate H/R enhanced in mitochondria the production and activity of reactive oxygen species scavengers, such as glutathione, manganese superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase. It was demonstrated that the maintenance of GSH-redox cycle by activation of glutathione reductase and NADP + -dependent isocitrate dehydrogenase is an integral part of the biochem... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585330 Sun, 31 May 2009 23:00:00 +0100 2585330 http://www.medworm.com/index.php?rid=2585329&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584029%26dopt%3DAbstract Authors: Fedina I, Velitchkova M, Georgieva K, Nedeva D, Cakırlar H The relationship between the greening stage of barley seedlings and their response to UV-B irradiation was studied. Etiolated barley seedlings ( Hordeum vulgare L., cv. Alfa) greened 12, 24 and 48 h were exposed to UV-B irradiation (312 nm) for 5 h. As a result of UV-B treatment the rate of CO 2 fixation and chlorophyll contents decreased but flavonoids, UV-B-induced compounds and carotenoids increased. The inhibition of photosynthesis in green plants was lower in comparison to greening ones. The 12 h greening plants were more sensitive to UV-B treatment than the plants greening 24 h and particularly 48 h, estimated by the quantum efficiency of PSII photochemistry and the oxygen production rate. The levels of fla... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585329 Sun, 31 May 2009 23:00:00 +0100 2585329 http://www.medworm.com/index.php?rid=2585328&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584030%26dopt%3DAbstract Authors: Hristozkova M, Geneva M, Stancheva I The influence of foliar feeding on the nitrogen assimilation in alfalfa plants under conditions of Mo shortage was studied. It was established that foliar fertilization with 0.3% solution of Agroleaf(R) resulted in increase of nitrogen fixation and nitrogen assimilation in the absence of Mo. Insufficient molybdenum supply leads to significant reduction of plant Mo content and nitrogen-fixing activity, while stress induced amino acids as alanine, GABA, threonine, proline and serine increased repeatedly. The negative effect of Mo deficiency on the enzyme activities related to the primary nitrogen assimilation (NR, GS, GOGAT) and plant growth diminished due to the foliar absorbed nutrients. PMID: 19584030 [PubMed - in process] (Source: Act... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585328 Sun, 31 May 2009 23:00:00 +0100 2585328 http://www.medworm.com/index.php?rid=2585327&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584031%26dopt%3DAbstract Authors: Yumurtaci A, Aydin Y, Uncuoglu AA Effects of salt stress on root growth, mitotic index, nuclear volume, vacuolization, nucleolar distortion and starch content were investigated in Turkish bread wheat ( Triticum aestivum L. cvs. Yildiz - salt sensitive, Dagdas - salt tolerant) and durum wheat ( Triticum durum L. cvs. C1252 - salt sensitive, Meramsalt tolerant) genotypes which were treated with 150 mM NaCI over a 6-day period. Salt treatment of wheat seedlings resulted in a decrease in root elongation and cell division in all genotypes at the 48 hours. According to controls, wheat root length decrease was 49% for Dagdas, 53.34% for Yildiz, 25.34% for Meram, 53.68% for C1252 at the 48 h. Mitotic index showed a more significant decrease in sensitive genotypes (1.24% for Yildiz, 0.... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585327 Sun, 31 May 2009 23:00:00 +0100 2585327 http://www.medworm.com/index.php?rid=2585326&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584032%26dopt%3DAbstract Authors: Taski-Ajdukovic K, Nagl N, Miladinovic D, Mikic A The plant regeneration from sunflower protoplast was achieved by protocols that considerably differ with respect to basal medium and concentration of growth regulators added. In most cases regeneration was restricted to a small proportion of genotypes. In this experiment, sunflower inbred line Ha-74A, with a high regeneration ability was used for protoplast isolation from etiolated hypocotyls. Isolated protoplasts were embedded in agarose droplets and cultured according to different regeneration protocols. The best results were obtained when protoplasts were cultured on L4 medium followed by transfer of regenerated microcalluses to solid regeneration media. Shoot regeneration was achieved by culture of calluses on SE regenerati... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585326 Sun, 31 May 2009 23:00:00 +0100 2585326 http://www.medworm.com/index.php?rid=2547616&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378919%26dopt%3DAbstract In conclusion, present data imply that the toxicity of paraoxon is both dose and duration dependent, which may even remain after the cessation of exposure. PMID: 19378919 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547616 Sun, 01 Mar 2009 00:00:00 +0100 2547616 http://www.medworm.com/index.php?rid=2547615&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378920%26dopt%3DAbstract Authors: Orun O, Rasmussen S, Gether U We have introduced tetracysteine motifs into different positions of the dopamine transporter (DAT) for specific FlAsH labeling. Two of the constructs expressed at the cell surface and were functional as determined by [3H] dopamine uptake experiments. The N-terminally modified transporter showed uptake levels comparable to the wild-type DAT, while the construct with tetracysteine motif at position 511 displayed an uptake level about 1/3 of its wild-type counterpart. In addition, these two transporter constructs were visualized on the cell surface following labeling with a fluorescent cocaine analog. YFP introduced into the same N-terminal position was also shown to have surface staining in agreement with activity tests. We propose that these two si... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547615 Sun, 01 Mar 2009 00:00:00 +0100 2547615 http://www.medworm.com/index.php?rid=2547614&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378921%26dopt%3DAbstract Authors: Zhu JQ, Yang WX Ultrastructure of mature spermatozoa of Estellarca olivacea was studied by transmission electron microscopy and its phylogenetic implications are discussed for the first time in this paper. The mature spermatozoon is composed of a head which contains a cone-shaped acrosome, a round nucleus and a tail region. The subacrosomal space is less electron dense which contains a homogeneous material. No axial rod and a basal plate were observed in subacrosomal space. No anterior invagination exists in the nucleus, but an inverted shallow V-shaped posterior invagination is visible. Nuclear lacunae could be seen clearly although the nucleus is highly condensed. Within the mid-piece of the spermatozoon there exist five spherical mitochondria while the long whip-like end po... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547614 Sun, 01 Mar 2009 00:00:00 +0100 2547614 http://www.medworm.com/index.php?rid=2547613&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378922%26dopt%3DAbstract Authors: Dikić D, Zidovec-Lepej S, Remenar A, Horvat-Knezević A, Benković V, Lisicić D, Sajli L, Springer O Prometryne is a methylthio-s-triazine herbicide used to control annual broadleaf and grass weeds in many cultivated plants. Significant traces are documented in environment, mainly water, soil and plants used for human and domestic animal nutrition. Data on the toxic effects of prometryne and other methylthio-s-triazine have scorcely been published. The goal of this study was to investigate if prometryne, applied orally, could induce DNA damage in mouse leukocytes, in subchronical in vivo experimental design. Three different doses of prometryne were applied per os repeatedly every 48 hours. After the 7th dose (day 14) and the 14th dose (day 28) blood leuco... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547613 Sun, 01 Mar 2009 00:00:00 +0100 2547613 http://www.medworm.com/index.php?rid=2547612&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378923%26dopt%3DAbstract Authors: Sobeková A, Holovská K, Lenártová V, Flesárová S, Javorský P The activities of the antioxidant and detoxifying enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR), glutathione-S-transferase (GST), and the content of thiobarbituric acid reactive substances (TBARS) were determined in the liver and kidney of rabbits after exposure to bendiocarb. In the liver, the activities of SOD, CAT and GR were not affected by bendiocarb. The induction or inhibition of isoenzymes of SOD (mainly MnSOD) were observed in the experimental groups. The activities of GSHPx-cum and GSHPx-H2O2 significantly decreased on the days 3 and 10 of the experiment. The activity of GST significantly increased on the da... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547612 Sun, 01 Mar 2009 00:00:00 +0100 2547612 http://www.medworm.com/index.php?rid=2547611&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378924%26dopt%3DAbstract Authors: Zwolak I, Zaporowska H In the present work, we investigated the cytotoxicity of vanadium and the influence of zinc and selenium on vanadium-dependent cell damage in the BALB/c 3T3 cell culture. Treatment of cells for 24 hours with medium containing 50, 100 and 200 microM NaVO3 caused a significant decrease in the cell viability as measured by MTT test. Furthermore, the assays for reactive oxygen species (NBT reduction and phenol red oxidation) demonstrated the increase in superoxide and hydrogen peroxide production. In the cotreatment studies, the cells were exposed to NaVO3 (50, 100 and 200 microM) in the presence of nontoxic concentrations of ZnCl2 (5 microM) or Na2SeO3 (0.5 microM). Following 24 h incubation, the cell viability (assessed in MTT assay) and reactive oxygen sp... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547611 Sun, 01 Mar 2009 00:00:00 +0100 2547611 http://www.medworm.com/index.php?rid=2547610&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378925%26dopt%3DAbstract The objective of this study was to determine whether the antioxidative effects of deprenyl can suppress apoptotic activity, with or without estradiol, in aged female rat kidney. Wistar Albino female rats were divided into six groups as follows; young (3 months old) control, aged (24 months old) control, aged deprenyl treated, aged estradiol treated, aged deprenyl plus estradiol treated and sham. All rats except for the sham group were injected for 21 days. Determination of oxidative stress parameter was performed spectrophotometrically. To detect apoptotic cells, TUNEL staining and caspase-3 immunohistochemistry were performed. Deprenyl and estradiol administration, alone or in combination, decreased significantly the levels of lipid peroxidation relative to aged control and sham-injected ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547610 Sun, 01 Mar 2009 00:00:00 +0100 2547610 http://www.medworm.com/index.php?rid=2547609&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378926%26dopt%3DAbstract Authors: Chater S, Douki T, Favier A, Sakly M, Abdelmelek H The research was conducted to investigate the toxic effects of cadmium chloride (CdCl2), administered during gestation period on female Wistar rats. Pregnant rats received CdCl2 (20 mg/l, orally) from Day 6 to Day 19 of pregnancy. Results showed that Cd treatment induced a decrease in body weight gain. The relative liver weight increased significantly, with a marked decrease of glycogen and total lipids content. The administration of Cd induced hepatotoxicity as indicated by elevations in plasma alanine aminotransferase (ALT), aspartate aminotransferase and lactate dehydrogenase (LDH) activities (p < 0.05). Treatment with CdCl2 caused a significant (p < 0.05) increase in glucose. A significant increase was observed in th... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547609 Sun, 01 Mar 2009 00:00:00 +0100 2547609 http://www.medworm.com/index.php?rid=2547608&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378927%26dopt%3DAbstract Authors: Jantová S, Letasiová S, Theiszová M, Palou M Fluorapatite (FA) is one of the inorganic constituents of bone or teeth used for hard tissue repairs and replacements. Fluor-hydroxyapatite (FHA) is a new synthetic composite that contains the same molecular concentration of OH(-) groups and F(-) ions. The aim of this experiment was to evaluate the cellular responses of murine fibroblast NIH-3T3 cells in vitro to solid solutions of FHA and FA and to compare them with the effect of hydroxyapatite (HA). We studied 24, 48 and 72 h effects of biomaterials on cell morphology, proliferation and cell cycle of NIH-3T3 cells by eluate assay. Furthermore, we examined the ability of FHA, FA and HA to induce cell death and DNA damage. Our cytotoxic/antiproliferative studies indic... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547608 Sun, 01 Mar 2009 00:00:00 +0100 2547608 http://www.medworm.com/index.php?rid=2547607&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378928%26dopt%3DAbstract Authors: Chaffai R, Seybou TN, Marzouk B, El Ferjani E A comparative analysis of fatty acid composition was conducted in maize (Zea mays L.) under copper and cadmium stress. The unsaturation level (double-bond index) of phosphatidylethanolamine (PE) and digalactosyldiacylglycerol (DGDG) was increased in response to both metal treatments, whereas the phosphatidylinositol (PI), the phosphatidylcholine (PC) showed no significant changes. The Cu-treated roots showed a marked increase (about 2-fold) in the phospholipid (PL) content, while the Cd-treated roots showed a slight but insignificant increase. The steryl lipid SL/PL ratio was markedly decreased in response to Cu stress, and therefore, may indicate an activated phospholipid biosynthesis and turnover, in response to damage caused by ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547607 Sun, 01 Mar 2009 00:00:00 +0100 2547607 http://www.medworm.com/index.php?rid=2547606&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378929%26dopt%3DAbstract Authors: Sanlibaba P, Buzrul S, Akkoç N, Alpas H, Akçelik M Survival curves of Lactococcus lactis subsp. lactis bacteriophage pll98 inactivated by heat were obtained at seven temperature values (50-80 degrees C) in M17 broth and skim milk. Deviations from first-order kinetics in both media were observed as sigmoidal shapes in the survival curves of pll98. An empirical model with four parameters was used to define the thermal inactivation. Number of parameters of the model was reduced from four to two in order to increase the robustness of the model. The reduced model produced comparable fits to the full model. Both the survival data and the calculations done using the reduced model (time necessary to reduce the number of phage pll98 six- or seven- log10) indicated that skim m... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547606 Sun, 01 Mar 2009 00:00:00 +0100 2547606 http://www.medworm.com/index.php?rid=2159002&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133504%26dopt%3DAbstract Authors: Shah AA, Hasan F, Hameed A, Ahmed S A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 degrees C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The differe... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2159002 Mon, 01 Dec 2008 05:00:00 +0100 2159002 http://www.medworm.com/index.php?rid=2099658&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133496%26dopt%3DAbstract We examined brain-suboesophageal ganglia and corpora cardiaca-corpora allata complexes of the cabbage moth, Mamestra brassicae, in order to obtain clues about possible peptide candidates which may be appropriate for the biological control of this pest. With the aid of bioassays, reversed phase high performance liquid chromatography, and mass spectrometry, five neuropeptides were unequivocally identified and the presence of a further three were inferred solely by comparing mass spectra with known peptides. Only one neuropeptide with adipokinetic capability was identified in M. brassicae. Data from the established homologous bioassay indicated that the cabbage moths rely on a lipid-based metabolism which is aided by an adipokinetic hormone (viz. Manse-AKH) that had previously been isolated i... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099658 Mon, 01 Dec 2008 05:00:00 +0100 2099658 http://www.medworm.com/index.php?rid=2099657&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133497%26dopt%3DAbstract Authors: Ray PP, Sengupta A, Chaudhuri-Sengupta S, Maiti BR The current study was undertaken to ascertain the effects of diverse stress on thyroid activity in soft-shelled turtles, Lissemys punctata punctata. The findings revealed that starvation (10 days), dehydration (10 days) or exposure to electric shock (12 volts for 15 seconds at an interval of 30 min for 3 h) caused significant decrease in the body weight (except in electric shock), relative weight, peripheral and central epithelial heights of the follicles and peroxidase activity of the thyroid gland of turtles. The degree of change in the values of these parameters was nearly same in all the stress experiments, indicating that there is not much difference in the degree of thyroid responses to diverse stress in turtles. It is s... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099657 Mon, 01 Dec 2008 05:00:00 +0100 2099657 http://www.medworm.com/index.php?rid=2099656&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133498%26dopt%3DAbstract Authors: Sahoo DK, Roy A, Chainy GB Accumulation of oxidative damage caused by reactive oxygen species (ROS) underlies fundamental changes found during aging. In the present study, age related effect on testicular mitochondrial oxidant generation and antioxidant defence profile was investigated in Wistar rats at 3 months (young adults), 12 months (old adults) and 24 months (senescent animals) of age. Mitochondrial oxidative stress parameters viz., lipid peroxidation (LPx), protein carbonylation, hydrogen peroxide (H2O2) generation and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), levels of total, oxidized (GSSG) and reduced glutathione (GSH) were studied to find out their roles in maintenance of mitochondrial glutathione redox pool ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099656 Mon, 01 Dec 2008 05:00:00 +0100 2099656 http://www.medworm.com/index.php?rid=2099655&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133499%26dopt%3DAbstract Authors: Szabó B, Lakatos A, Koszegi T, Botz L We aimed to understand the effects of water stress on the alkaloid production in various developmental stages of poppy plants and the effect of stress on the alkaloids content in the capsules. Three stages of the life cycle of Papaver somniferum L. were selected in our studies: Rosette, Flowering and Lancing developmental stages. Four types of water conditions were examined: Control, Withdrawal of Water, 50% Water Supply and Inundation. The morphological monitoring, results of Relative Water Content and proline content were used as indicators of stress. The result of the measurements in poppy leaves show that the secondary metabolites dramatically respond to these stress conditions. The constant water supply was beneficial for the acc... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099655 Mon, 01 Dec 2008 05:00:00 +0100 2099655 http://www.medworm.com/index.php?rid=2099654&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133500%26dopt%3DAbstract Authors: Kerrigan JE, Ragunath C, Kandra L, Gyémánt G, Lipták A, Jánossy L, Kaplan JB, Ramasubbu N Bacteria in a biofilm are enmeshed in a self-synthesized extracellular polysaccharide matrix (PGA), which is a linear polymer of beta(1,6)-linked N-acetylglucosamine (GlcNAc) residues. Dispersin B (DspB), a soluble glycoside hydrolase produced by the periodontal pathogen Actinobacillus actinomycetemcomitans degrades PGA. The enzyme DspB is an alpha/beta TIM-barrel protein and belongs to family 20 glycosyl hydrolases members. The enzyme activity of DspB with regard to its substrate specificity towards beta(1,6)-linked GlcNAc polymers and its endo/exo character was investigated through ligand docking and the hydrolysis of synthetic oligosaccharides. Ligand docking analys... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099654 Mon, 01 Dec 2008 05:00:00 +0100 2099654 http://www.medworm.com/index.php?rid=2099653&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133501%26dopt%3DAbstract Authors: Arican E, Gozukirmizi N Changes in relative water content (RWC), lipid peroxidation, proline and antioxidant system in relation to the tolerance to oxidative stress enzymes mediated high pressure were investigated in Hordeum vulgare L. cv. Tokak. For this purpose, mature embryos cultured on MS media were treated in a hyperbaric oxygenation chamber (approx. 59.06 feets, 2 kp/cm2) with pure oxygen for 60 minutes/day for a growth period of ten days in a plant growth chamber. Constitutive activities of SOD, APOX, GR and POX were higher in hyperbaric oxygenated (HBO) explants, being 96.07%, 28.57%, 77.77% and 54.14% for the 5th days; 95.78%, 40%, 37.5%, and 94.98% for the 10th days of culture, respectively, than in the control plants. Increase in SOD activity was also shown on poly... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099653 Mon, 01 Dec 2008 05:00:00 +0100 2099653 http://www.medworm.com/index.php?rid=2099652&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133502%26dopt%3DAbstract Authors: Sheval EV, Kazhura YI, Poleshuk NA, Lazareva EM, Smirnova EA, Maximova NP, Polyakov VY The extensive use of herbicides in agriculture becomes an important factor in environmental pollution, especially in case of slowly degradable compounds. Some agents act on plants during a long period of time, even if a very low concentration of the herbicide remains in the soil. Here, we investigated the toxicological effect of a low concentration of dinitroaniline herbicide, trifluralin, on growing seedlings of Hordeum vulgare L. Trifluralin in concentration of 1 microg/ml inhibited root growth. The mitotic activity of meristematic cells was suppressed due to the retardation of metaphase progression--alteration that can be caused by cytoskeleton disorder. Using antibodies to alpha-tubulin,... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099652 Mon, 01 Dec 2008 05:00:00 +0100 2099652 http://www.medworm.com/index.php?rid=2099651&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133503%26dopt%3DAbstract Authors: Stoyanova Z, Zozikova E, Poschenrieder C, Barcelo J, Doncheva S The effect of exogenously applied silicon (Si) on plant growth, lipid peroxidation, total phenolic compounds and non-protein thiols was studied in two maize varieties (Zea mays L. vars. Kneja 605, 434) differing in sensitivity to excess manganese (Mn). Based on the density of brown spots per leaf area and relative shoot weight (RSW) used to define Mn tolerance var. Kneja 434 was found to be more Mn-tolerant than Kneja 605. The lipid peroxidation level and total phenolic compounds were enhanced with increasing Mn concentration in the nutrient solution. In addition, the Mn-sensitive var. Kneja 605 with markedly expressed first visible Mn toxicity symptoms had higher levels of total phenolic acids than var. Kneja 434... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099651 Mon, 01 Dec 2008 05:00:00 +0100 2099651 http://www.medworm.com/index.php?rid=2099650&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133504%26dopt%3DAbstract Authors: Shah AA, Hasan F, Hameed A, Ahmed S A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 degrees C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The differe... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099650 Mon, 01 Dec 2008 05:00:00 +0100 2099650 http://www.medworm.com/index.php?rid=2099649&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133505%26dopt%3DAbstract Authors: Lazova G, Yonova P The changes in the polypeptide patterns of soluble proteins of wheat flag leaves after treatment with two synthetic cytokinin-like substances 3-CP-2-PU [1-(3-chlorophenyl)-3-(2-pyridyl)urea] and 3-CP-4-MPU [1-(3-chlorophenyl)-3-(4-methyl-2-pyridyl)urea] was studied. Both compounds increased differentially in quantity and composition most individual polypeptides identified compared to the non-treated leaves. While 3-CP-2-PU increased only the polypeptide quantity, 3-CP-4-MPU led to the appearance of new polypeptide with Mw of 51 kDa. PMID: 19133505 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099649 Mon, 01 Dec 2008 05:00:00 +0100 2099649 http://www.medworm.com/index.php?rid=1770153&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18773505%26dopt%3DAbstract Authors: PMID: 18773505 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1770153 Sun, 07 Sep 2008 07:32:08 +0100 1770153 http://www.medworm.com/index.php?rid=1767543&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652368%26dopt%3DAbstract Authors: Chistopolsky IA, Vorontsov DD, Sakharov DA To detect neuroactive factors in biological fluids, we have previously elaborated a moveable biosensor, the preparation of isolated neuron penetrated with recording microelectrode. Here, we report that, in the pond snail, signals emanated from a central pattern generator (CPG) for feeding and detected by such biosensor correspond to long-term changes in motor output of the CPG as recorded from buccal nerves. The results suggest that combined recording from motor nerves, neurons in situ and the neuron-based biosensors may be a useful tool in studies of the mechanisms underlying network organisation. PMID: 18652368 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767543 Sat, 06 Sep 2008 07:31:30 +0100 1767543 http://www.medworm.com/index.php?rid=1767542&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652369%26dopt%3DAbstract Authors: Dyakonova TL, Dyakonova VE NO-generating drugs (SNP, SNAP, NaNO2) activated the standard buccal rhythm and simultaneously slowed down the endogenous rhythm of the NO-synthesizing neuron B2 which enhances gut motility. In the B2, these drugs caused giant hyperpolarizing waves strongly associated with the buccal cycles. Hyperpolarizing waves on the B2 could also be induced by prolonged stimulation of the B2. The inhibitor of NO-synthase L-NNA or the NO scavanger PTIO abolished giant hyperpolarizing waves, depolarised the B2 and activated fast bursting in the B2. The results suggest that NO may be involved in coordination of radula and foregut movements. PMID: 18652369 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767542 Sat, 06 Sep 2008 07:31:30 +0100 1767542 http://www.medworm.com/index.php?rid=1767541&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652372%26dopt%3DAbstract Authors: Balog G, Elekes K Organization of the innervation of the buccal region by 5-HT-immunoreactive (IR) elements was investigated in the pond snail, Lymnaea stagnalis, with special attention to developmental aspects. A gradual maturation is characteristic for the 5-HT-IR muscle innervation, appearing first by late (E80-90%) embryogenesis. It runs parallel with the muscle development and the maturation of the 5-HTergic innervation in the buccal ganglia, peaking by the mid-postembryogenesis (P3) with the presence of a 5-HT-IR network in the buccal mass and rich innervation in the buccal ganglia, including axo-somatic contacts. The whole process seems to match with the appearance of the adult-like feeding (radula protrusion). PMID: 18652372 [PubMed - indexed for MEDLINE] (Source: ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767541 Sat, 06 Sep 2008 07:31:30 +0100 1767541 http://www.medworm.com/index.php?rid=1767540&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652373%26dopt%3DAbstract Authors: Sadamoto H, Serfozo Z, Ito E The serotonin transporter, SERT, is reported as a key molecule that regulates serotonergic neurotransmission. In the present study, we analyzed the localization of Lymnaea SERT (LymSERT) mRNA-containing neurons by in situ hybridization using frozen sections of the central nervous system (CNS) of Lymnaea. To precisely demonstrate the distribution of LymSERT mRNA-containing neurons, colocalization with serotonin immunoreactivity was also examined. The results showed that LymSERT mRNA was constitutively expressed and localized in the serotonin-containing neurons in the CNS. PMID: 18652373 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767540 Sat, 06 Sep 2008 07:31:30 +0100 1767540