MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Acta Biologica Hungarica' source. Sun, 21 Mar 2010 17:00:35 +0100 http://www.medworm.com/index.php?rid=3328084&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194094%26dopt%3DAbstract This study was performed to investigate the effects of L-thyroxine treatment on plasma vasopressin (AVP) levels in rats with hypothyroidism induced by propylthiouracil (PTU). Animals were separated into three groups each having 6 rats: control, PTU, PTU+L-thyroxine groups. Then, the groups were further divided into 3 sub-groups including 6 rats (a; basal, b; hypertonic stimulated and c; hypovolemic stimulated). At the end of the experiments all rats were decapitated in order to obtain plasma samples for analysis in terms of Hct, osmolality, TT 3 , TT 4 and vasopressin. Haematocrit (Hct) levels were the highest in hypovolemic stimulated sub-group (P < 0.001). Osmolality levels were higher in hypertonic stimulated sub-groups (P < 0.001). Total T 3 and T 4 values were the lowest in the ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328084 Mon, 01 Mar 2010 00:00:00 +0100 3328084 http://www.medworm.com/index.php?rid=3328083&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194095%26dopt%3DAbstract Authors: Said Ali K, Ferencz A, Nemcsók J, Hermesz E Heat shock proteins (HSPs) and metallothioneins (MTs) play important roles in protection against environmental stressors. The present study analyzes and compares the regulation of heat shock ( hsp70, hsc70-1 and hsp90alpha ) and metallothionein (MT-1 and MT-2) genes in the heart of common carp, in response to elevated temperature, cold shock and exposure to several heavy metal ions (As 3+ , Cd 2+ and Cu 2+ ), in whole-animal experiments. Among these metals, arsenate proved to be the most potent inducer of the examined stress genes; the hsp90alpha and MT-1 mRNA levels were elevated 11- and 10-fold, respectively, after a 24-h exposure. In contrast, Cd 2+ at 10 mg/L had no impact on the expression of hsp90alpha , and the MT genes a... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328083 Mon, 01 Mar 2010 00:00:00 +0100 3328083 http://www.medworm.com/index.php?rid=3328082&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194096%26dopt%3DAbstract Authors: Hegyi A, Urbányi B, Kovács M, Lefler KK, Gál J, Hoitsy G, Horváth A The transportation of rainbow trout in the presence of the anesthetic clove oil was investigated. Before the transportation tests, an acute experiment was conducted to verify that removal of the fish from the water for one minute does not significantly increase the glucose or cortisol concentration of the blood plasma. In the main experiment two different transportation conditions were compared: transport in water only and in water with anesthetic. During transportation without addition of clove oil, blood plasma glucose and cortisol concentrations changed significantly. The concentration of glucose increased from 4.92 mmol/L prior to transportation to 6.16 mmol/L and values similar to the ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328082 Mon, 01 Mar 2010 00:00:00 +0100 3328082 http://www.medworm.com/index.php?rid=3328081&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194097%26dopt%3DAbstract Authors: Eissa FI, Zidan NA The renal- and hepato-toxicity induced by abamectin pesticide (Vertimec) and a commercial form of a bio-insecticide Bacillus thuringiensis (Agerin) in male albino rats were evaluated. Blood picture and blood glucose level were investigated. Male albino rats were administered dietary doses each equivalent to 1/10 or 1/100 of the LD 50 values of each toxicant for 30 consecutive days. Abamectin was found to pose risks of renal- and hepato-toxicity in rats, since the biochemical parameters of liver function (i.e. aspartate aminotransferase activity, alanine aminotransferase activity, acid phosphatase activity, albumin, and total protein levels) and kidney function (uric acid and creatinine concentration) were severely affected. These effects were verified by his... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328081 Mon, 01 Mar 2010 00:00:00 +0100 3328081 http://www.medworm.com/index.php?rid=3328080&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194098%26dopt%3DAbstract Authors: Szentmihályi K, Gere A, Szöllösi-Varga I, Blázovics A, Jasztrab S, Ladó K, Then M Plant drug mixtures are widely used in the adjuvant therapy of type 2 diabetes mellitus for the prevention of complications. The drug mixtures generally contribute to the efficiency of the therapy and may also reduce undesirable side effects. Two herbal extracts (lyophilized aqueous extracts of plant drug mixtures 1: Myrtilli folium, Phaseoli fructus sine seminibus and 2: Myrtilli folium, Phaseoli fructus sine seminibus, Salviae folium ) were investigated in in vitro rat models. The content of bioactive constituents (polyphenol, flavonoid and vitamin C) in plant drug mixtures and lyophilized samples was evaluated. The antioxidant activity of lyophilized extracts was deter... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328080 Mon, 01 Mar 2010 00:00:00 +0100 3328080 http://www.medworm.com/index.php?rid=3328079&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194099%26dopt%3DAbstract Authors: Sun L, Yu F, Xu Z, Zeng X, Ferreri M, Han B Objective of this study was to assess the quantification of osteocalcin (OCN) expression by ovine osteoblasts cultured with different concentrations of sodium fluoride (F) and sodium selenite (Se) to evaluate the interaction of these agents on OCN expression in vitro . We wanted to demonstrate a possible protective effect of selenium on the toxic effect of fluoride. Osteoblasts were isolated by complete trypsin and collagenase digestion from ovine calvarial bone and cultured in DMEM supplemented with 15% FBS at 37 degrees C in a humidified 5% CO 2 incubator. Identified osteoblasts were divided into one control group (C) and eight experimental groups, which were exposed to different concentrations of sodium fluoride (F; 0, 0.5, 1 mM) ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328079 Mon, 01 Mar 2010 00:00:00 +0100 3328079 http://www.medworm.com/index.php?rid=3328078&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194100%26dopt%3DAbstract We present novel data for ABH antigen reactivity in reproductive organs of vertebrates which have not been studied so far. Our results suggest that HBGA are evolutionary stable structures, most possibly related to vitellogenesis in oocytes with high yolk content. The tendency observed in A and B antigen expression is mostly associated with the processes of gamete differentiation and vitellogenesis, rather than with the evolutionary development of vertebrate species. PMID: 20194100 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328078 Mon, 01 Mar 2010 00:00:00 +0100 3328078 http://www.medworm.com/index.php?rid=3328077&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194101%26dopt%3DAbstract Authors: Sharifi G, Ebrahimzadeh H Among the different concentrations of Thidiazuron (TDZ) and between the two media Gamborg (B5) and Murashige and Skoog (MS), the highest frequency of shoot formation could be seen in the MS medium with TDZ concentration of 4.54 muM. Among the different concentrations of Naphtalene acetic acid (NAA) and Benzyl adenine (BA) in the two aforementioned media, the maximum proliferation and rooting of saffron shoots were obtained in a B5 medium containing 2.22 muM NAA and 2.68 muM BA. Peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), esterase (EST) and polyphenoloxidase (PPO) measurements proved that all the enzymes had a similar pattern of changes, according to which their concentrations increased in the first stages of development and then decr... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328077 Mon, 01 Mar 2010 00:00:00 +0100 3328077 http://www.medworm.com/index.php?rid=3328076&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194102%26dopt%3DAbstract Authors: Borowiak K, Zbierska J, Drapikowska M Tropospheric ozone causes visible injuries in selected plants. Some plant species have been recognized as resistant and sensitive to ozone. Differences in the response to ozone of two kinds of plants are interesting because of practical implications. Resistant cultivars (without visible symptoms) will be more valuable for agriculture, forestry and horticulture. It is, however, necessary to find out the reason of the occurrence of the visible symptoms. The presented studies focused on selected morphological and anatomical differences between well-known ozone-sensitive (Bel W3) and -resistant (Bel B) cultivars of tobacco plants. Daily growth of the sensitive cultivar was higher than that of the resistant one. This tendency was also valid for... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328076 Mon, 01 Mar 2010 00:00:00 +0100 3328076 http://www.medworm.com/index.php?rid=3328075&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194103%26dopt%3DAbstract Authors: Takó M, Farkas E, Lung S, Krisch J, Vágvölgyi C, Papp T Extracellular beta-glucosidase activity of 94 strains, representing 24 species of the genera Gilbertella, Mucor, Rhizomucor , and Rhizopus was evaluated in submerged culture and under solid state fermentation on wheat bran. Gilbertella persicaria G1 isolate showed the highest activity (70.9 U ml -1 ) followed by other Gilbertella (58.6-59.0 U ml -1 ) and Rhizomucor miehei isolates (29.2-42.0 U ml -1 ). Optimum temperature for enzyme production was 25 degrees C for Gilbertella and Mucor , and 30 degrees C for Rhizomucor and Rhizopus strains. Enzymes of R. miehei strains proved to be thermotolerant preserving up to 92.8% residual activity after heating to 75 degrees C in the presence of cellobiose substrate. ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328075 Mon, 01 Mar 2010 00:00:00 +0100 3328075 http://www.medworm.com/index.php?rid=3328074&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20194104%26dopt%3DAbstract Importance of acidic phosphatase activity in P supply and Gonyostomum semen Ehrenbergh (Raphidophyta) occurrence in a Hungarian peat bog, Keleméri Kis-Mohos (Ne Hungary). Acta Biol Hung. 2010 Mar;61(1):111-21 Authors: Grigorszky I, Dévai G, Kiss KT, Tóthmérész B, Gligora M, Plenkovic-Moraj A, Kraj K, Béres VB, Schnitchen C, Borics G, Nagy AS Phosphatase enzymes are capable of releasing phosphate through cleavage of phosphoester bonds. The seasonal importance of this process was examined by using a model substrate paranitrophenylphosphate and the Michaelis-Menten equation to estimate the release rate of PO 4 -P from phosphomonoesters. The seasonal occurrence of phosphomonoesters and acid phosphatase activity was used to estimate the velocity of phos... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3328074 Mon, 01 Mar 2010 00:00:00 +0100 3328074 http://www.medworm.com/index.php?rid=3105499&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015826%26dopt%3DAbstract Authors: Jójárt J, Jójárt I, Boda K, Gálfi M, Mihály A, B-Baldauf Z, Vecsernyés M We investigated the distribution of oxytocin in rat spinal cord using immunocytochemistry and radioimmunoassay (RIA). Each segment of the spinal cord from cervical to coccygeal contained oxytocin-immunoreactive fibers. The Rexed laminae I and II of the dorsal horn showed moderate to intense immunoreactivity. A dense network was found around the central canal where some fibers apposed the ependyma. The autonomic centers of the spinal cord at the thoracolumbar and sacral segments were heavily innervated. Few fibers were found around the motoneurons. In the white matter, the immunoreactivity was localized mainly in the dorsal part of the lateral funiculus, in the pars funic... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105499 Tue, 01 Dec 2009 00:00:00 +0100 3105499 http://www.medworm.com/index.php?rid=3105498&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015827%26dopt%3DAbstract Authors: Bódi N, Battonyai I, Talapka P, Fekete E, Bagyánszki M Nitrergic myenteric neurons are especially susceptible to the development of neuropathy in functional gastrointestinal disorders. Investigations of the similarities and dissimilarities in the organization of nitrergic neurons in the various mammalian species are therefore important in an effort to determine the extent to which the results obtained in different animal models can be generalized. In the present work, the density and the spatial organization of the nitrergic neurons in the myenteric plexus of the duodenum were investigated in 7 mammalian species. After nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry, the Plexus Pattern Analysis software (PPAs) was applied to count the ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105498 Tue, 01 Dec 2009 00:00:00 +0100 3105498 http://www.medworm.com/index.php?rid=3105497&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015828%26dopt%3DAbstract Authors: Lukács A, Szabó A, Papp A, Vezér T 3-nitropropionic acid (3-NP), a substance used for modelling Huntington's disease, was given to male Wistar rats in a single 20 mg/kg b.w. dose, and the resulting behavioral alterations in spontaneous locomotor activity were measured after 30 minutes. To detect the involvement of neurotransmitter systems in this immediate effect, the NMDA antagonist MK-801 (0.8 mg/kg); as well as an agonist, quinpirole (QP, 5 mg/kg) and an antagonist, sulpiride (SP, 80 mg/kg) of the dopamine D2 receptors, were given before 3-NP to separate groups of rats. Controls were given saline. All substances were injected ip. 3-NP decreased the rats' locomotor, especially vertical, activity, whereas local activity was increased. Based on the further chang... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105497 Tue, 01 Dec 2009 00:00:00 +0100 3105497 http://www.medworm.com/index.php?rid=3105496&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015829%26dopt%3DAbstract Authors: Krsková L, Vrabcová M, Talarovicová A, Zeman M The renin-angiotensin system (RAS) plays an important role in the development of hypertension and has serious consequences on behaviour. The aim of our study was to investigate the effect of hypertension, induced by up-regulated RAS, on the exploration, anxiety-related behaviour and object recognition in laboratory rats. In the experiment, 12 weeks old normotensive Sprague-Dawley (SD) and hypertensive TGR(mREN2)27 (TGR) male rats with up-regulated RAS were used. In the open-field test, the TGR rats were less active in ambulating, rearing and sniffing and more active in self-grooming and urinating than SD ones. In the elevated plus-maze test, the TGR rats showed lower frequency of total arm entries, closed arm entrie... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105496 Tue, 01 Dec 2009 00:00:00 +0100 3105496 http://www.medworm.com/index.php?rid=3105495&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015830%26dopt%3DAbstract Authors: Nadia BH, Wided K, Kheira B, Hassiba R, Lamia B, Rhouati S, Alyane M, Zellagui A, Lahouel M This paper reports an investigation of the ability of propolis extract (a resinous substance collected by honeybees from various plant sources) to restore the collapse of mitochondrial membrane potential induced by ferulenol, a sesquiterpene prenylated coumarin derivative isolated from the plant Ferula vesceritensis . We show that ferulenol was able to induce the permeability transition pore (PTP) opening. This effect is caused by the interaction of the compound with the mitochondrial respiratory chain, more particularly by the fall of membrane potential and the inhibition of complex II. We have previously demonstrated that this inhibition results from a limitation of electron transfers... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105495 Tue, 01 Dec 2009 00:00:00 +0100 3105495 http://www.medworm.com/index.php?rid=3105494&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015831%26dopt%3DAbstract This study was undertaken to examine the effect of melatonin on leptin production using male C57BL/6 adult mice treated with or without daily melatonin supplements (10 mug/mL) in drinking water for 1 month. In addition, in vitro experiments using adipose tissue fragments derived from epididymal fat pads of adult mice incubated with or without melatonin (1 nM) administration were also conducted. The results showed that melatonin-supplemented mice had significantly higher plasma leptin levels than control mice. However, melatonin incubation did not cause any marked changes in the amount of leptin secreted from adipose tissue fragments. Our findings from this study indicate that melatonin does not affect leptin secretion via mouse adipose tissue. Nevertheless, melatonin could still influence ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105494 Tue, 01 Dec 2009 00:00:00 +0100 3105494 http://www.medworm.com/index.php?rid=3105493&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015832%26dopt%3DAbstract In this study, in order to elucidate the toxic nuclear effects of dichlorvos, freshly isolated human peripheral blood lymphocytes were incubated with 5, 10, 20, 40, 80 and 100 mug/mL of dichlorvos. According to the results, dichlorvos induced micronuclei, decreased the mitotic and replication indexes. It is a genotoxic product causing chromosomal damage (an increase in micronucleus) and cell death (decrease in mitotic and replication indexes). PMID: 20015832 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105493 Tue, 01 Dec 2009 00:00:00 +0100 3105493 http://www.medworm.com/index.php?rid=3105492&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015833%26dopt%3DAbstract Authors: Terzi R, Saruhan N, Sağlam A, Nar H, Kadioğlu A We studied the changes in antioxidant system and chlorophyll fluorescence parameters in post-stress emerging Ctenanthe setosa (Rosc.) Eichler (Marantaceae) plants (PSE plants) having reduced leaf area under drought stress causing leaf rolling and re-watering. PSE plants were compared to primary stressed plants (PS) in previous studies. The parameters were measured at different visual leaf rolling scores from 1 to 4 (1 is unrolled, 4 is tightly rolled and the others is intermediate form). Water potentials and stomatal conductance of leaves were gradually decreased during leaf rolling. Similarly, maximum quantum efficiency of open PS II center and quantum yield of PS II decreased during the rolling period. Non-photochem... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105492 Tue, 01 Dec 2009 00:00:00 +0100 3105492 http://www.medworm.com/index.php?rid=3105491&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015834%26dopt%3DAbstract Authors: Atia A, Debez A, Rabhi M, Smaoui A, Abdelly C Interaction of salinity, nitrate, light, and seed weight on the germination of Crithmum maritimum was investigated. Seeds of three size categories were germinated at 0-200 mM NaCl with either 0, 5 or 20 mM KNO 3 . Experiments were done under darkness, white light, or red light. Regardless of seed weight, germination was maximal in distilled water. Under salinity, the smallest seeds showed the highest germination percentage. Salt impact was amplified by darkness, but was mitigated by nitrate supply, red light and their combination. At the same PPFD, germination of T2 seeds was higher, when exposed to red light than under white light, suggesting that germination was more influenced by the light type than by the PPFD. As a whole, not ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105491 Tue, 01 Dec 2009 00:00:00 +0100 3105491 http://www.medworm.com/index.php?rid=3105490&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20015835%26dopt%3DAbstract Authors: Nasim SA, Mujib A, Rashmi K, Samar F, Junaid A, Mahmooduzzafar Direct somatic embryo formation (without intervening callus) from garlic clove basal tissue was induced in which the influence of plant growth regulators (PGRs) on various explants was examined. Medium added with 2.0 mg/l 6-benzylaminopurine (BAP) and 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) were the most effective PGR combination for somatic embryo induction. It induced embryos directly in 85.5% of the basal clove explant. Callus induction was also obtained from other parts of explant and 2.0 mg/l 2,4-D induced callusing in 86.5% of the inoculated explants. Protein, amino acid and alliin content were measured in callus and in embryos. Somatic embryos had more soluble protein and free amino acid compared to... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3105490 Tue, 01 Dec 2009 00:00:00 +0100 3105490 http://www.medworm.com/index.php?rid=2735096&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700383%26dopt%3DAbstract This study suggests the probable interaction between Ang II and opioid system in the VTA. PMID: 19700383 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735096 Thu, 27 Aug 2009 04:00:28 +0100 2735096 http://www.medworm.com/index.php?rid=2735095&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700384%26dopt%3DAbstract Authors: Cherici Camargo IC, Barreiros De Souza R, Fátima Paccola Mesquita S, Chuffa LG, Frei F The study was conducted to analyze the histology of the ovaries of adults rats treated with steroids, and submitted or not to physical effort. The control group consisted of females submitted to physical effort and sedentary females, both of which received a physiological solution of 0.9% saline. Treated females, sedentary or not, received 6 mg/kg of body weight of nandrolone decanoate. The steroid and physiological solution were administered intraperitoneally, with a single injection per week for 4 consecutive weeks. The applied physical effort was swimming (20 minutes daily, 5 days/week, for the 4 weeks of treatment). Serial sections (5 mum) of ovaries were prepared for histological e... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735095 Thu, 27 Aug 2009 04:00:26 +0100 2735095 http://www.medworm.com/index.php?rid=2735094&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700385%26dopt%3DAbstract Authors: Lukasik I, Goławska S, Leszczyński B Effects of some dietary o -dihydroxyphenols on biochemical markers of oxidative stress within tissues of Sitobion avenae (Fabricius), (Homoptera, Aphididae) and Rhopalosiphum padi (Linnaeus), (Homoptera, Aphididae) has been studied. Among the studied aphid morphs the highest concentration of total thiols, hydrogen peroxide and lipid peroxidation products (TBARS) was noted for winged adults (alatae). Higher content of H 2 O 2 was observed within tissues of the oligophagous species R. padi while the monophagous species S. avenae had higher TBARS and total thiols concentration. Aphids exposed the dietary pro-oxidative o -dihydroxyphenols (quercetin, caffeic acid, chlorogenic acid) demonstrated significantly lower concentration of t... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735094 Thu, 27 Aug 2009 04:00:22 +0100 2735094 http://www.medworm.com/index.php?rid=2735093&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700386%26dopt%3DAbstract Authors: Nciri R, Allagui MS, Vincent C, Croute F, Elfeki A Lithium salts are efficiently used for treatment of psychiatric disorders. However, prolonged treatment frequently involves adverse side-effects. In the present work, effects of lithium carbonate administration on some biochemical parameters were studied in male mice. Lithium carbonate (20, 40 or 80 mg/kg body weight, corresponding to 3.77, 7.54 or 15.08 mg Li element/kg body weight, respectively) was injected daily for 14 or 28 days. The following parameters were recorded: water consumption, body weight, lithium and testosterone serum concentrations, activities of catalase (CAT), superoxide-dismutase (SOD) and glutathione-peroxidase (GPX) and level of lipid peroxidation (expressed as TBARS) in liver was performed. Lithium tre... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735093 Thu, 27 Aug 2009 04:00:20 +0100 2735093 http://www.medworm.com/index.php?rid=2735092&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700387%26dopt%3DAbstract Authors: Chen Q, Cao M, Xiang WL, Sun Q, Zhang J, Hou RT, Yan ZY, Yang ZR, Liu J, Zhao J The forward and reverse cDNA subtractive libraries before and after the toxic effect of alpha-amanitin were constructed by suppression subtractive hybridization and randomly selected clones from each subtractive library were screened by PCR and dot blot hybridization. A total of 85 genes with altered expression were finally identified, with 41 genes from the forward library and 44 from the reverse library. Subsequently, the antagonistic effects of candidate traditional Chinese medicines were evaluated based on the genetic transcription levels of the genes with significant altered expression, including Catnbeta, Flt3-L, IL-7r and Rpo2-4. The results indicated that Silybum marianum (L.) Gaert and Gan... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735092 Thu, 27 Aug 2009 04:00:17 +0100 2735092 http://www.medworm.com/index.php?rid=2735091&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700388%26dopt%3DAbstract Authors: Refaey M, Kováts N, Kárpáti A, Thury P Whole effluent toxicity is most often considered as a static parameter. However, toxicity might change as degradation processes, especially biodegradation goes by and intermediate products appear. These intermediates can even be more toxic than the original effluent was, posing higher risk to the ecosystem of the recipient water body. In our test series it was assessed how toxicity of a municipal wastewater sample changes during biodegradation taking into consideration different temperature regimes (10, 20 and 30 degrees C). Results proved our null hypothesis: after the high initial toxicity of the fresh effluent sample toxicity did show a further increase. Biodegradation resulted in toxicity reduction only after an approx.... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735091 Thu, 27 Aug 2009 04:00:15 +0100 2735091 http://www.medworm.com/index.php?rid=2735090&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700389%26dopt%3DAbstract In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4-6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA - 6-benzylaminopurine; NAA - alpha-naphthaleneacetic acid; TDZ - 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 muM NAA + 8.87 muM BA and 2.68 muM NAA + 17.74 muM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 muM NAA + 4.44 muM BA produced somatic embryos at a frequency of 38%. PMID... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735090 Thu, 27 Aug 2009 04:00:12 +0100 2735090 http://www.medworm.com/index.php?rid=2735089&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700390%26dopt%3DAbstract In this study, induction of regenerable callus from mature embryos in eight Turkish barley varieties was analysed by using different plant growth regulators (PGRs). Varying concentrations (0.5-4 mg l -1 ) of 2,4-dichlorophenoxyacetic acid (2,4-D) and dicamba (3,6-dichloro- o -anisic acid) were tested for callus induction from mature embryos. Highest percent of callus induction was observed in Bornova 92 variety (98.3%) on MS medium supplemented with 4 mg l -1 dicamba. Calli were transferred to regeneration media with 0.5 mg l -1 dicamba, 0.5 mg l -1 zeatin riboside (ZR) and 2 mg l -1 thidiazuron (TDZ). Low concentrations of dicamba induced multiple shoots during callus regeneration. When the effect of precultivation with 2,4-D or dicamba on the shoot induction were evaluated, lower concent... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735089 Thu, 27 Aug 2009 04:00:10 +0100 2735089 http://www.medworm.com/index.php?rid=2735088&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700391%26dopt%3DAbstract Authors: Tóth V, Antal K, Gyémánt G, Miskei M, Pócsi I, Emri T Coprogen production of Neurospora crassa was dependent on glucose, aspartate and iron contents as well as on initial pH of the culture media. Surplus iron and acidic pH hindered the production of coprogen as well as the transcription of the sid1 gene (NCU07117) encoding putative L-ornithine-N 5 -monooxygenase, the first enzyme in the coprogen biosynthetic pathway. High glucose (40 g/l) and aspartate (21 g/l) concentrations were beneficial for coprogen synthesis, but neither glucose nor aspartate affected the sid1 transcription. Moreover, efficient coprogen production was observed after glucose had been consumed, which suggested that N. crassa accumulated iron even in non-growing, carbon-starving cultures... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735088 Thu, 27 Aug 2009 04:00:08 +0100 2735088 http://www.medworm.com/index.php?rid=2735087&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19700392%26dopt%3DAbstract Authors: Zenke P, Maróti-Agóts A, Pádár Z, Zöldág L Demand for correct and cost-effective genetic-based identification and parentage control has increasing importance in domestic animals, including dogs. In our study the applicability of a canine hyperpolymorphic microsatellite marker - which localized in the WILMS-TF (tumor factor) gene - was examined in mixed breed and purebred canine populations. The redesigned and shortened amplicons were genotyped using an allelic ladder which was constructed from sequence verified fragments. The nomenclature for allele calling based on repetition structures is suitable for international comparisons. Our study justified the potential use and efficiency of the marker D18S12 in parentage control. PMID: 19700392 [Pub... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2735087 Thu, 27 Aug 2009 04:00:05 +0100 2735087 http://www.medworm.com/index.php?rid=2585335&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584023%26dopt%3DAbstract Authors: Pekcetin C, Kiray M, Ergur BU, Tugyan K, Bagriyanik HA, Erbil G, Baykara B, Camsari UM Cerebral ischemia leads to cognitive decline and neuronal damage in the hippocampus. Reactive oxygen species (ROS) play an important role in the neuronal loss after cerebral ischemia and reperfusion injury. Carnosine has both antioxidant and neuroprotective effects against ROS. In the present study, the effects of carnosine on oxidative stress, apoptotic neuronal cell death and spatial memory following transient cerebral ischemia in rats were investigated. Transient ischemia was induced by occlusion of right common carotid artery of rats for 30 min and reperfusion for 24 h or 1 week. Rats received intraperitoneal injection of 250 mg/kg carnosine or saline 30 min prior to experiment. Determin... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585335 Sun, 31 May 2009 23:00:00 +0100 2585335 http://www.medworm.com/index.php?rid=2585334&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584024%26dopt%3DAbstract Authors: Ali KS, Ferencz A, Deér AK, Nemcsók J, Hermesz E The expression pattern of two metallothionein (MT) genes in response to temperature shock and exposure to Cd 2+ was investigated in the brain of common carp ( Cyprinus carpio ), in whole-animal experiments. The changes in the levels of MT-1 and MT-2 mRNA in the olfactory lobe, midbrain and cerebellum were followed by semiquantitative RT-PCR. The inducibility of the two MT genes was brain regionand stressor-specific. Cd 2+ affected mostly the expression of MT-2, while the level of the MT-1 transcript did not change significantly in any of the brain regions examined. Moreover, the MT-2 expression was regulated spatially; MT-2 was induced significantly more strongly in the olfactory lobe than in the cerebellum or midbrain... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585334 Sun, 31 May 2009 23:00:00 +0100 2585334 http://www.medworm.com/index.php?rid=2585333&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584025%26dopt%3DAbstract Authors: Gülsoy N, Colak S This paper describes various aspects of previtellogenic oocyte growth in sharpsnout seabream, Diplodus puntazzo , is an important marine culture fish species in the Mediterranean. The ultrastructural characteristics of nuclear morphology, nuclear-cytoplasmic ratio and the starting of the follicle envelope formation were described in detail. These cells do not significantly differ from those of the other teleost species. The ultrastructural aspects provide new information on the reproductive biology of Sparidae. PMID: 19584025 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585333 Sun, 31 May 2009 23:00:00 +0100 2585333 http://www.medworm.com/index.php?rid=2585332&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584026%26dopt%3DAbstract Authors: Holecková B, Siviková K, Dianovský J Bendiocarb is a carbamate broad-spectrum insecticide used to control disease vectors such as mosquitoes and flies, as well as household and agricultural pests. Nowadays, only few papers reporting cytogenetic or possible genotoxic effect of this insecticide on mammalian cells are available. In the present study 24-hour exposure to bendiocarbamate at concentrations ranging from 20 to 160 mug/ml was used for investigation of unstable chromosomal aberrations (CA), sister chromatid exchanges (SCE) and stable chromosomal aberration induction in cultured bovine peripheral lymphocytes. The slight but no significant increase of chromatide breaks frequency was observed after the exposure of lymphocytes to 80 mug/ml of bendiocarb. At th... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585332 Sun, 31 May 2009 23:00:00 +0100 2585332 http://www.medworm.com/index.php?rid=2585331&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584027%26dopt%3DAbstract In this study Maximum Tolerated Dose (MTD) was obtained according to CSGMT protocol presented by the Environmental Mutagen Society of Japan. The positive group received mitomycin C in dose of 0.5 mg/kg. The peripheral blood samples before treatment (zero time) were considered as negative control. The MTD of Hypiron was 12 ml/kg and for Chamomilla was 10 ml/kg. Doses of MTD, 1/2 MTD and 1/4 MTD were considered for test groups. Then blood samples were prepared 48 hours after first administration of drugs and kept on precoated Acridine orange slides. The scoring of micronucleated reticulocytes were carried out per 2000 counted reticulocytes in each slide by fluorescent microscope. The results were statistically analyzed. Results of Hypiran were compared with negative control group and the P v... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585331 Sun, 31 May 2009 23:00:00 +0100 2585331 http://www.medworm.com/index.php?rid=2585330&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584028%26dopt%3DAbstract Authors: Gonchar O, Mankovskaya I In an experimental model, it was shown that repetitive periods of hypoxia/reoxygenation (H/R) [5 cycles of 5 min hypoxia (12% O 2 in N 2 ) followed by 15 min normoxia, daily for three weeks] attenuated basal and stimulated in vitro lipid peroxidation, as well as H 2 O 2 production in liver and brain mitochondria of rats exposed to acute severe hypoxia. Adaptation to moderate H/R enhanced in mitochondria the production and activity of reactive oxygen species scavengers, such as glutathione, manganese superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase. It was demonstrated that the maintenance of GSH-redox cycle by activation of glutathione reductase and NADP + -dependent isocitrate dehydrogenase is an integral part of the biochem... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585330 Sun, 31 May 2009 23:00:00 +0100 2585330 http://www.medworm.com/index.php?rid=2585329&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584029%26dopt%3DAbstract Authors: Fedina I, Velitchkova M, Georgieva K, Nedeva D, Cakırlar H The relationship between the greening stage of barley seedlings and their response to UV-B irradiation was studied. Etiolated barley seedlings ( Hordeum vulgare L., cv. Alfa) greened 12, 24 and 48 h were exposed to UV-B irradiation (312 nm) for 5 h. As a result of UV-B treatment the rate of CO 2 fixation and chlorophyll contents decreased but flavonoids, UV-B-induced compounds and carotenoids increased. The inhibition of photosynthesis in green plants was lower in comparison to greening ones. The 12 h greening plants were more sensitive to UV-B treatment than the plants greening 24 h and particularly 48 h, estimated by the quantum efficiency of PSII photochemistry and the oxygen production rate. The levels of fla... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585329 Sun, 31 May 2009 23:00:00 +0100 2585329 http://www.medworm.com/index.php?rid=2585328&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584030%26dopt%3DAbstract Authors: Hristozkova M, Geneva M, Stancheva I The influence of foliar feeding on the nitrogen assimilation in alfalfa plants under conditions of Mo shortage was studied. It was established that foliar fertilization with 0.3% solution of Agroleaf(R) resulted in increase of nitrogen fixation and nitrogen assimilation in the absence of Mo. Insufficient molybdenum supply leads to significant reduction of plant Mo content and nitrogen-fixing activity, while stress induced amino acids as alanine, GABA, threonine, proline and serine increased repeatedly. The negative effect of Mo deficiency on the enzyme activities related to the primary nitrogen assimilation (NR, GS, GOGAT) and plant growth diminished due to the foliar absorbed nutrients. PMID: 19584030 [PubMed - in process] (Source: Act... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585328 Sun, 31 May 2009 23:00:00 +0100 2585328 http://www.medworm.com/index.php?rid=2585327&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584031%26dopt%3DAbstract Authors: Yumurtaci A, Aydin Y, Uncuoglu AA Effects of salt stress on root growth, mitotic index, nuclear volume, vacuolization, nucleolar distortion and starch content were investigated in Turkish bread wheat ( Triticum aestivum L. cvs. Yildiz - salt sensitive, Dagdas - salt tolerant) and durum wheat ( Triticum durum L. cvs. C1252 - salt sensitive, Meramsalt tolerant) genotypes which were treated with 150 mM NaCI over a 6-day period. Salt treatment of wheat seedlings resulted in a decrease in root elongation and cell division in all genotypes at the 48 hours. According to controls, wheat root length decrease was 49% for Dagdas, 53.34% for Yildiz, 25.34% for Meram, 53.68% for C1252 at the 48 h. Mitotic index showed a more significant decrease in sensitive genotypes (1.24% for Yildiz, 0.... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585327 Sun, 31 May 2009 23:00:00 +0100 2585327 http://www.medworm.com/index.php?rid=2585326&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19584032%26dopt%3DAbstract Authors: Taski-Ajdukovic K, Nagl N, Miladinovic D, Mikic A The plant regeneration from sunflower protoplast was achieved by protocols that considerably differ with respect to basal medium and concentration of growth regulators added. In most cases regeneration was restricted to a small proportion of genotypes. In this experiment, sunflower inbred line Ha-74A, with a high regeneration ability was used for protoplast isolation from etiolated hypocotyls. Isolated protoplasts were embedded in agarose droplets and cultured according to different regeneration protocols. The best results were obtained when protoplasts were cultured on L4 medium followed by transfer of regenerated microcalluses to solid regeneration media. Shoot regeneration was achieved by culture of calluses on SE regenerati... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2585326 Sun, 31 May 2009 23:00:00 +0100 2585326 http://www.medworm.com/index.php?rid=2547616&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378919%26dopt%3DAbstract In conclusion, present data imply that the toxicity of paraoxon is both dose and duration dependent, which may even remain after the cessation of exposure. PMID: 19378919 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547616 Sun, 01 Mar 2009 00:00:00 +0100 2547616 http://www.medworm.com/index.php?rid=2547615&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378920%26dopt%3DAbstract Authors: Orun O, Rasmussen S, Gether U We have introduced tetracysteine motifs into different positions of the dopamine transporter (DAT) for specific FlAsH labeling. Two of the constructs expressed at the cell surface and were functional as determined by [3H] dopamine uptake experiments. The N-terminally modified transporter showed uptake levels comparable to the wild-type DAT, while the construct with tetracysteine motif at position 511 displayed an uptake level about 1/3 of its wild-type counterpart. In addition, these two transporter constructs were visualized on the cell surface following labeling with a fluorescent cocaine analog. YFP introduced into the same N-terminal position was also shown to have surface staining in agreement with activity tests. We propose that these two si... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547615 Sun, 01 Mar 2009 00:00:00 +0100 2547615 http://www.medworm.com/index.php?rid=2547614&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378921%26dopt%3DAbstract Authors: Zhu JQ, Yang WX Ultrastructure of mature spermatozoa of Estellarca olivacea was studied by transmission electron microscopy and its phylogenetic implications are discussed for the first time in this paper. The mature spermatozoon is composed of a head which contains a cone-shaped acrosome, a round nucleus and a tail region. The subacrosomal space is less electron dense which contains a homogeneous material. No axial rod and a basal plate were observed in subacrosomal space. No anterior invagination exists in the nucleus, but an inverted shallow V-shaped posterior invagination is visible. Nuclear lacunae could be seen clearly although the nucleus is highly condensed. Within the mid-piece of the spermatozoon there exist five spherical mitochondria while the long whip-like end po... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547614 Sun, 01 Mar 2009 00:00:00 +0100 2547614 http://www.medworm.com/index.php?rid=2547613&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378922%26dopt%3DAbstract Authors: Dikić D, Zidovec-Lepej S, Remenar A, Horvat-Knezević A, Benković V, Lisicić D, Sajli L, Springer O Prometryne is a methylthio-s-triazine herbicide used to control annual broadleaf and grass weeds in many cultivated plants. Significant traces are documented in environment, mainly water, soil and plants used for human and domestic animal nutrition. Data on the toxic effects of prometryne and other methylthio-s-triazine have scorcely been published. The goal of this study was to investigate if prometryne, applied orally, could induce DNA damage in mouse leukocytes, in subchronical in vivo experimental design. Three different doses of prometryne were applied per os repeatedly every 48 hours. After the 7th dose (day 14) and the 14th dose (day 28) blood leuco... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547613 Sun, 01 Mar 2009 00:00:00 +0100 2547613 http://www.medworm.com/index.php?rid=2547612&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378923%26dopt%3DAbstract Authors: Sobeková A, Holovská K, Lenártová V, Flesárová S, Javorský P The activities of the antioxidant and detoxifying enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR), glutathione-S-transferase (GST), and the content of thiobarbituric acid reactive substances (TBARS) were determined in the liver and kidney of rabbits after exposure to bendiocarb. In the liver, the activities of SOD, CAT and GR were not affected by bendiocarb. The induction or inhibition of isoenzymes of SOD (mainly MnSOD) were observed in the experimental groups. The activities of GSHPx-cum and GSHPx-H2O2 significantly decreased on the days 3 and 10 of the experiment. The activity of GST significantly increased on the da... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547612 Sun, 01 Mar 2009 00:00:00 +0100 2547612 http://www.medworm.com/index.php?rid=2547611&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378924%26dopt%3DAbstract Authors: Zwolak I, Zaporowska H In the present work, we investigated the cytotoxicity of vanadium and the influence of zinc and selenium on vanadium-dependent cell damage in the BALB/c 3T3 cell culture. Treatment of cells for 24 hours with medium containing 50, 100 and 200 microM NaVO3 caused a significant decrease in the cell viability as measured by MTT test. Furthermore, the assays for reactive oxygen species (NBT reduction and phenol red oxidation) demonstrated the increase in superoxide and hydrogen peroxide production. In the cotreatment studies, the cells were exposed to NaVO3 (50, 100 and 200 microM) in the presence of nontoxic concentrations of ZnCl2 (5 microM) or Na2SeO3 (0.5 microM). Following 24 h incubation, the cell viability (assessed in MTT assay) and reactive oxygen sp... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547611 Sun, 01 Mar 2009 00:00:00 +0100 2547611 http://www.medworm.com/index.php?rid=2547610&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378925%26dopt%3DAbstract The objective of this study was to determine whether the antioxidative effects of deprenyl can suppress apoptotic activity, with or without estradiol, in aged female rat kidney. Wistar Albino female rats were divided into six groups as follows; young (3 months old) control, aged (24 months old) control, aged deprenyl treated, aged estradiol treated, aged deprenyl plus estradiol treated and sham. All rats except for the sham group were injected for 21 days. Determination of oxidative stress parameter was performed spectrophotometrically. To detect apoptotic cells, TUNEL staining and caspase-3 immunohistochemistry were performed. Deprenyl and estradiol administration, alone or in combination, decreased significantly the levels of lipid peroxidation relative to aged control and sham-injected ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547610 Sun, 01 Mar 2009 00:00:00 +0100 2547610 http://www.medworm.com/index.php?rid=2547609&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378926%26dopt%3DAbstract Authors: Chater S, Douki T, Favier A, Sakly M, Abdelmelek H The research was conducted to investigate the toxic effects of cadmium chloride (CdCl2), administered during gestation period on female Wistar rats. Pregnant rats received CdCl2 (20 mg/l, orally) from Day 6 to Day 19 of pregnancy. Results showed that Cd treatment induced a decrease in body weight gain. The relative liver weight increased significantly, with a marked decrease of glycogen and total lipids content. The administration of Cd induced hepatotoxicity as indicated by elevations in plasma alanine aminotransferase (ALT), aspartate aminotransferase and lactate dehydrogenase (LDH) activities (p < 0.05). Treatment with CdCl2 caused a significant (p < 0.05) increase in glucose. A significant increase was observed in th... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547609 Sun, 01 Mar 2009 00:00:00 +0100 2547609 http://www.medworm.com/index.php?rid=2547608&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378927%26dopt%3DAbstract Authors: Jantová S, Letasiová S, Theiszová M, Palou M Fluorapatite (FA) is one of the inorganic constituents of bone or teeth used for hard tissue repairs and replacements. Fluor-hydroxyapatite (FHA) is a new synthetic composite that contains the same molecular concentration of OH(-) groups and F(-) ions. The aim of this experiment was to evaluate the cellular responses of murine fibroblast NIH-3T3 cells in vitro to solid solutions of FHA and FA and to compare them with the effect of hydroxyapatite (HA). We studied 24, 48 and 72 h effects of biomaterials on cell morphology, proliferation and cell cycle of NIH-3T3 cells by eluate assay. Furthermore, we examined the ability of FHA, FA and HA to induce cell death and DNA damage. Our cytotoxic/antiproliferative studies indic... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547608 Sun, 01 Mar 2009 00:00:00 +0100 2547608 http://www.medworm.com/index.php?rid=2547607&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378928%26dopt%3DAbstract Authors: Chaffai R, Seybou TN, Marzouk B, El Ferjani E A comparative analysis of fatty acid composition was conducted in maize (Zea mays L.) under copper and cadmium stress. The unsaturation level (double-bond index) of phosphatidylethanolamine (PE) and digalactosyldiacylglycerol (DGDG) was increased in response to both metal treatments, whereas the phosphatidylinositol (PI), the phosphatidylcholine (PC) showed no significant changes. The Cu-treated roots showed a marked increase (about 2-fold) in the phospholipid (PL) content, while the Cd-treated roots showed a slight but insignificant increase. The steryl lipid SL/PL ratio was markedly decreased in response to Cu stress, and therefore, may indicate an activated phospholipid biosynthesis and turnover, in response to damage caused by ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547607 Sun, 01 Mar 2009 00:00:00 +0100 2547607 http://www.medworm.com/index.php?rid=2547606&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19378929%26dopt%3DAbstract Authors: Sanlibaba P, Buzrul S, Akkoç N, Alpas H, Akçelik M Survival curves of Lactococcus lactis subsp. lactis bacteriophage pll98 inactivated by heat were obtained at seven temperature values (50-80 degrees C) in M17 broth and skim milk. Deviations from first-order kinetics in both media were observed as sigmoidal shapes in the survival curves of pll98. An empirical model with four parameters was used to define the thermal inactivation. Number of parameters of the model was reduced from four to two in order to increase the robustness of the model. The reduced model produced comparable fits to the full model. Both the survival data and the calculations done using the reduced model (time necessary to reduce the number of phage pll98 six- or seven- log10) indicated that skim m... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2547606 Sun, 01 Mar 2009 00:00:00 +0100 2547606 http://www.medworm.com/index.php?rid=2159002&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133504%26dopt%3DAbstract Authors: Shah AA, Hasan F, Hameed A, Ahmed S A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 degrees C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The differe... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2159002 Mon, 01 Dec 2008 05:00:00 +0100 2159002 http://www.medworm.com/index.php?rid=2099658&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133496%26dopt%3DAbstract We examined brain-suboesophageal ganglia and corpora cardiaca-corpora allata complexes of the cabbage moth, Mamestra brassicae, in order to obtain clues about possible peptide candidates which may be appropriate for the biological control of this pest. With the aid of bioassays, reversed phase high performance liquid chromatography, and mass spectrometry, five neuropeptides were unequivocally identified and the presence of a further three were inferred solely by comparing mass spectra with known peptides. Only one neuropeptide with adipokinetic capability was identified in M. brassicae. Data from the established homologous bioassay indicated that the cabbage moths rely on a lipid-based metabolism which is aided by an adipokinetic hormone (viz. Manse-AKH) that had previously been isolated i... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099658 Mon, 01 Dec 2008 05:00:00 +0100 2099658 http://www.medworm.com/index.php?rid=2099657&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133497%26dopt%3DAbstract Authors: Ray PP, Sengupta A, Chaudhuri-Sengupta S, Maiti BR The current study was undertaken to ascertain the effects of diverse stress on thyroid activity in soft-shelled turtles, Lissemys punctata punctata. The findings revealed that starvation (10 days), dehydration (10 days) or exposure to electric shock (12 volts for 15 seconds at an interval of 30 min for 3 h) caused significant decrease in the body weight (except in electric shock), relative weight, peripheral and central epithelial heights of the follicles and peroxidase activity of the thyroid gland of turtles. The degree of change in the values of these parameters was nearly same in all the stress experiments, indicating that there is not much difference in the degree of thyroid responses to diverse stress in turtles. It is s... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099657 Mon, 01 Dec 2008 05:00:00 +0100 2099657 http://www.medworm.com/index.php?rid=2099656&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133498%26dopt%3DAbstract Authors: Sahoo DK, Roy A, Chainy GB Accumulation of oxidative damage caused by reactive oxygen species (ROS) underlies fundamental changes found during aging. In the present study, age related effect on testicular mitochondrial oxidant generation and antioxidant defence profile was investigated in Wistar rats at 3 months (young adults), 12 months (old adults) and 24 months (senescent animals) of age. Mitochondrial oxidative stress parameters viz., lipid peroxidation (LPx), protein carbonylation, hydrogen peroxide (H2O2) generation and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), levels of total, oxidized (GSSG) and reduced glutathione (GSH) were studied to find out their roles in maintenance of mitochondrial glutathione redox pool ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099656 Mon, 01 Dec 2008 05:00:00 +0100 2099656 http://www.medworm.com/index.php?rid=2099655&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133499%26dopt%3DAbstract Authors: Szabó B, Lakatos A, Koszegi T, Botz L We aimed to understand the effects of water stress on the alkaloid production in various developmental stages of poppy plants and the effect of stress on the alkaloids content in the capsules. Three stages of the life cycle of Papaver somniferum L. were selected in our studies: Rosette, Flowering and Lancing developmental stages. Four types of water conditions were examined: Control, Withdrawal of Water, 50% Water Supply and Inundation. The morphological monitoring, results of Relative Water Content and proline content were used as indicators of stress. The result of the measurements in poppy leaves show that the secondary metabolites dramatically respond to these stress conditions. The constant water supply was beneficial for the acc... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099655 Mon, 01 Dec 2008 05:00:00 +0100 2099655 http://www.medworm.com/index.php?rid=2099654&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133500%26dopt%3DAbstract Authors: Kerrigan JE, Ragunath C, Kandra L, Gyémánt G, Lipták A, Jánossy L, Kaplan JB, Ramasubbu N Bacteria in a biofilm are enmeshed in a self-synthesized extracellular polysaccharide matrix (PGA), which is a linear polymer of beta(1,6)-linked N-acetylglucosamine (GlcNAc) residues. Dispersin B (DspB), a soluble glycoside hydrolase produced by the periodontal pathogen Actinobacillus actinomycetemcomitans degrades PGA. The enzyme DspB is an alpha/beta TIM-barrel protein and belongs to family 20 glycosyl hydrolases members. The enzyme activity of DspB with regard to its substrate specificity towards beta(1,6)-linked GlcNAc polymers and its endo/exo character was investigated through ligand docking and the hydrolysis of synthetic oligosaccharides. Ligand docking analys... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099654 Mon, 01 Dec 2008 05:00:00 +0100 2099654 http://www.medworm.com/index.php?rid=2099653&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133501%26dopt%3DAbstract Authors: Arican E, Gozukirmizi N Changes in relative water content (RWC), lipid peroxidation, proline and antioxidant system in relation to the tolerance to oxidative stress enzymes mediated high pressure were investigated in Hordeum vulgare L. cv. Tokak. For this purpose, mature embryos cultured on MS media were treated in a hyperbaric oxygenation chamber (approx. 59.06 feets, 2 kp/cm2) with pure oxygen for 60 minutes/day for a growth period of ten days in a plant growth chamber. Constitutive activities of SOD, APOX, GR and POX were higher in hyperbaric oxygenated (HBO) explants, being 96.07%, 28.57%, 77.77% and 54.14% for the 5th days; 95.78%, 40%, 37.5%, and 94.98% for the 10th days of culture, respectively, than in the control plants. Increase in SOD activity was also shown on poly... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099653 Mon, 01 Dec 2008 05:00:00 +0100 2099653 http://www.medworm.com/index.php?rid=2099652&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133502%26dopt%3DAbstract Authors: Sheval EV, Kazhura YI, Poleshuk NA, Lazareva EM, Smirnova EA, Maximova NP, Polyakov VY The extensive use of herbicides in agriculture becomes an important factor in environmental pollution, especially in case of slowly degradable compounds. Some agents act on plants during a long period of time, even if a very low concentration of the herbicide remains in the soil. Here, we investigated the toxicological effect of a low concentration of dinitroaniline herbicide, trifluralin, on growing seedlings of Hordeum vulgare L. Trifluralin in concentration of 1 microg/ml inhibited root growth. The mitotic activity of meristematic cells was suppressed due to the retardation of metaphase progression--alteration that can be caused by cytoskeleton disorder. Using antibodies to alpha-tubulin,... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099652 Mon, 01 Dec 2008 05:00:00 +0100 2099652 http://www.medworm.com/index.php?rid=2099651&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133503%26dopt%3DAbstract Authors: Stoyanova Z, Zozikova E, Poschenrieder C, Barcelo J, Doncheva S The effect of exogenously applied silicon (Si) on plant growth, lipid peroxidation, total phenolic compounds and non-protein thiols was studied in two maize varieties (Zea mays L. vars. Kneja 605, 434) differing in sensitivity to excess manganese (Mn). Based on the density of brown spots per leaf area and relative shoot weight (RSW) used to define Mn tolerance var. Kneja 434 was found to be more Mn-tolerant than Kneja 605. The lipid peroxidation level and total phenolic compounds were enhanced with increasing Mn concentration in the nutrient solution. In addition, the Mn-sensitive var. Kneja 605 with markedly expressed first visible Mn toxicity symptoms had higher levels of total phenolic acids than var. Kneja 434... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099651 Mon, 01 Dec 2008 05:00:00 +0100 2099651 http://www.medworm.com/index.php?rid=2099650&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133504%26dopt%3DAbstract Authors: Shah AA, Hasan F, Hameed A, Ahmed S A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 degrees C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The differe... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099650 Mon, 01 Dec 2008 05:00:00 +0100 2099650 http://www.medworm.com/index.php?rid=2099649&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19133505%26dopt%3DAbstract Authors: Lazova G, Yonova P The changes in the polypeptide patterns of soluble proteins of wheat flag leaves after treatment with two synthetic cytokinin-like substances 3-CP-2-PU [1-(3-chlorophenyl)-3-(2-pyridyl)urea] and 3-CP-4-MPU [1-(3-chlorophenyl)-3-(4-methyl-2-pyridyl)urea] was studied. Both compounds increased differentially in quantity and composition most individual polypeptides identified compared to the non-treated leaves. While 3-CP-2-PU increased only the polypeptide quantity, 3-CP-4-MPU led to the appearance of new polypeptide with Mw of 51 kDa. PMID: 19133505 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2099649 Mon, 01 Dec 2008 05:00:00 +0100 2099649 http://www.medworm.com/index.php?rid=1770153&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18773505%26dopt%3DAbstract Authors: PMID: 18773505 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1770153 Sun, 07 Sep 2008 07:32:08 +0100 1770153 http://www.medworm.com/index.php?rid=1767543&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652368%26dopt%3DAbstract Authors: Chistopolsky IA, Vorontsov DD, Sakharov DA To detect neuroactive factors in biological fluids, we have previously elaborated a moveable biosensor, the preparation of isolated neuron penetrated with recording microelectrode. Here, we report that, in the pond snail, signals emanated from a central pattern generator (CPG) for feeding and detected by such biosensor correspond to long-term changes in motor output of the CPG as recorded from buccal nerves. The results suggest that combined recording from motor nerves, neurons in situ and the neuron-based biosensors may be a useful tool in studies of the mechanisms underlying network organisation. PMID: 18652368 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767543 Sat, 06 Sep 2008 07:31:30 +0100 1767543 http://www.medworm.com/index.php?rid=1767542&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652369%26dopt%3DAbstract Authors: Dyakonova TL, Dyakonova VE NO-generating drugs (SNP, SNAP, NaNO2) activated the standard buccal rhythm and simultaneously slowed down the endogenous rhythm of the NO-synthesizing neuron B2 which enhances gut motility. In the B2, these drugs caused giant hyperpolarizing waves strongly associated with the buccal cycles. Hyperpolarizing waves on the B2 could also be induced by prolonged stimulation of the B2. The inhibitor of NO-synthase L-NNA or the NO scavanger PTIO abolished giant hyperpolarizing waves, depolarised the B2 and activated fast bursting in the B2. The results suggest that NO may be involved in coordination of radula and foregut movements. PMID: 18652369 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767542 Sat, 06 Sep 2008 07:31:30 +0100 1767542 http://www.medworm.com/index.php?rid=1767541&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652372%26dopt%3DAbstract Authors: Balog G, Elekes K Organization of the innervation of the buccal region by 5-HT-immunoreactive (IR) elements was investigated in the pond snail, Lymnaea stagnalis, with special attention to developmental aspects. A gradual maturation is characteristic for the 5-HT-IR muscle innervation, appearing first by late (E80-90%) embryogenesis. It runs parallel with the muscle development and the maturation of the 5-HTergic innervation in the buccal ganglia, peaking by the mid-postembryogenesis (P3) with the presence of a 5-HT-IR network in the buccal mass and rich innervation in the buccal ganglia, including axo-somatic contacts. The whole process seems to match with the appearance of the adult-like feeding (radula protrusion). PMID: 18652372 [PubMed - indexed for MEDLINE] (Source: ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767541 Sat, 06 Sep 2008 07:31:30 +0100 1767541 http://www.medworm.com/index.php?rid=1767540&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652373%26dopt%3DAbstract Authors: Sadamoto H, Serfozo Z, Ito E The serotonin transporter, SERT, is reported as a key molecule that regulates serotonergic neurotransmission. In the present study, we analyzed the localization of Lymnaea SERT (LymSERT) mRNA-containing neurons by in situ hybridization using frozen sections of the central nervous system (CNS) of Lymnaea. To precisely demonstrate the distribution of LymSERT mRNA-containing neurons, colocalization with serotonin immunoreactivity was also examined. The results showed that LymSERT mRNA was constitutively expressed and localized in the serotonin-containing neurons in the CNS. PMID: 18652373 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767540 Sat, 06 Sep 2008 07:31:30 +0100 1767540 http://www.medworm.com/index.php?rid=1767539&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652376%26dopt%3DAbstract Authors: Kobayashi S, Hattori M, Ito E Gamma-aminobutyric acid (GABA)-containing neurons are capable of controlling network oscillations and their patterns in the widely divergent species. In the terrestrial slug, Limax valentianus, oscillatory activity of the procerebrum neurons is considered to encode the odor information. Previous studies showed that GABA is present in the central nervous system and may be involved in the control of neuronal activity of Limax. In the present study, we examined the effects of GABA on the generation of oscillatory activity in the procerebrum. The results suggest that the GABAergic synaptic transmission may be involved in the oscillatory neural network of the procerebrum. PMID: 18652376 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungari... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767539 Sat, 06 Sep 2008 07:31:30 +0100 1767539 http://www.medworm.com/index.php?rid=1767538&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652378%26dopt%3DAbstract Authors: Suzuki H, Horikoshi T, Sakakibara M Lymnaea are capable of learning an association between light and rotation. The conditioning paradigm to produce this association and the unconditioned escape response are the same for both Lymnaea and Hermissenda, but the underlying neural mechanisms appear to differ between the two species due to differences in the organization and function of their visuo- and vestibular-sensory systems. The RPeD11 interneuron in Lymnaea controls withdrawal behavior. We analyzed the electrophysiologic characteristics of the RPeD11 and observed an increase in cell excitability; that is, spontaneous activity of the postsynaptic potential in the RpeD11 was increased in conditioned animals after learning acquisition. PMID: 18652378 [PubMed - indexed for MED... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767538 Sat, 06 Sep 2008 07:31:30 +0100 1767538 http://www.medworm.com/index.php?rid=1767537&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652379%26dopt%3DAbstract Authors: Karasawa T, Sato N, Horikoshi T, Sakakibara M Though adult Lymnaea are bimodal breathers, young animals breathe mainly through the skin and adults through the lung. Operant conditioning changes adult breathing behavior from aerial to cutaneous. We hypothesized that this behavioral change is caused by alterations in the neuronal circuit during both development and conditioning. We focused our study on whether the synaptic connection between RPeD1 and RPA6 neurons is modulated during development and conditioning. Our findings indicated that the RPeD1 has an excitatory monosynaptic contact with the RPA6 in young naive and operantly-conditioned adult animals. The relationship of this contact was well correlated with their respiratory behavior. PMID: 18652379 [PubMed - indexed ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767537 Sat, 06 Sep 2008 07:31:30 +0100 1767537 http://www.medworm.com/index.php?rid=1767536&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652380%26dopt%3DAbstract Authors: Tsyganov VV In a reduced preparation of Planorbarius corneus consisting of the CNS and mantle complex, both the dopamine precursor L-DOPA and the serotonin precursor 5-HTP have been found to be able to induce and maintain rhythmic pneumostome (PN) movements coupled, in a neurotransmitter-specific manner, to fictive cyclic locomotion. Following transection of the pedal commissures, the pharmacologically induced movements of the PN were coordinated with the locomotory rhythm generated by the left pedal ganglion. PMID: 18652380 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767536 Sat, 06 Sep 2008 07:31:30 +0100 1767536 http://www.medworm.com/index.php?rid=1767535&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652384%26dopt%3DAbstract Authors: Chaban AK, Voronezhskaya EE The role of transient larval FMRFa-ergic neurons of the freshwater snails, Helisoma trivolvis and Lymnaea stagnalis, in osmoregulation and neurodifferentiation was investigated. It was shown that: (i) FMRFa and an FMRFa antibody do not reach their targets when injected into the egg capsule; (ii) long-term incubations of the embryos in neomycin and hyperosmotic solutions of sea water, NaCl and sucrose during the definite developmental stages lead to a special malformation-hydropia; (iii) hydropia coincides with an extensive larval FMRFa-ergic nervous system; (iv) the increased level of FMRFa causes earlier serotonin synthesis by the neurons of the visceral loop ganglia. PMID: 18652384 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungari... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767535 Sat, 06 Sep 2008 07:31:30 +0100 1767535 http://www.medworm.com/index.php?rid=1767534&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652395%26dopt%3DAbstract Authors: Tolstenkov OO, Terenina N, Gustafsson M, Serbina E, Kreshchenko ND, Maklakova L, Jashina A Serotoninergic and FMRFamidergic components of the nervous system were examined in cercaria from different types using immunocytochemical techniques interfaced with confocal scanning laser microscopy. Cercariae from 9 families were studied - Opisthorchis felineus, Parafasciolopsis fasciolaemorpha, Echinochasmus coaxatus, Echinoparyphium aconiatum, Notocotylus attenuatus, Psilotrema tuberculata, Plagiorchis sp., Cyathocotyle bithyniae, Diplostomum chromatophorum. The results show that 5-HT-IR and FMRFamide-IR occur in all types of cercariae, regardless of what morphological, taxonomic and biological group they belong to. Small differences in the patterns of 5-HT-IR and FMRFamide-IR were o... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767534 Sat, 06 Sep 2008 07:31:30 +0100 1767534 http://www.medworm.com/index.php?rid=1767533&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652397%26dopt%3DAbstract In this study, we investigated a novel function of TK as an enhancer of oocyte growth. Ci-TK-R is expressed specifically in the Ciona vitellogenic oocytes. Moreover, administration of Ci-TK to the Ciona ovary resulted in upregulation of gene expression and enzymatic activity of several proteases. Moreover, maturation of the Ciona oocytes from the vitellogenic stage to the post-vitellogenic stage was induced in the presence of Ci-TK, which was completely blocked by addition of protease inhibitors. PMID: 18652397 [PubMed - indexed for MEDLINE] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767533 Sat, 06 Sep 2008 07:31:30 +0100 1767533 http://www.medworm.com/index.php?rid=1767532&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652398%26dopt%3DAbstract In this study, we show the functional modulation of Ci-GnRHR-1 via heterodimerization with the orphan receptor subtype, Ci-GnRHR-4. The dimerization between Ci-GnRHR-1 and R-4 was detected by co-immunoprecipitation and immunoblot analysis. Binding assays confirmed the binding of t-GnRHs to Ci-GnRHR-1 but not to R-4, and verified no alternation in ligand-binding affinity between Ci-GnRHR-1 homodimer and Ci-GnRHRI&4 heterodimer. The heterodimer was found to stimulate the elevation of intracellular calcium, time-extension of ERK phosphorylation, and up-regulation of cell proliferation, all in a ligand specific manner, compared with the Ci-GnRHR-1 homodimer. In combination, these results indicated that Ci-GnRHR-4 is not an inactive receptor, but a modulatory factor for Ci-GnRHR-1 in C. int... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1767532 Sat, 06 Sep 2008 07:31:30 +0100 1767532 http://www.medworm.com/index.php?rid=1656752&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652364%26dopt%3DAbstract Authors: Elekes K PMID: 18652364 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656752 Sun, 27 Jul 2008 06:42:42 +0100 1656752 http://www.medworm.com/index.php?rid=1656751&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652365%26dopt%3DAbstract Authors: Kiss T Voltage-dependent sodium channels have a decisive role in the generation of action potentials (AP) in many types of cells. In addition to the fast inactivating Na-current, associated with AP generation, the Na-channel can give rise to a noninactivating or persistent Na-current. The latter current generally comprises up to 5% of the transient current having important physiological consequences. It was established that persistent Na-currents have functional significance in setting the membrane potential in a subthreshold range regulating by this way dendritic depolarisations, repetitive firing and enhancing synaptic transmission. Voltage dependent sodium channel genes have been identified in a variety of invertebrates, as well as mammalian and nonmammalian vertebrates. It... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656751 Sun, 27 Jul 2008 06:42:42 +0100 1656751 http://www.medworm.com/index.php?rid=1656750&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652366%26dopt%3DAbstract Authors: Tsai MC How messengers modulate the shifting of spontaneously generated action potential into bursts of potentials (BoP) is studied electrophysiologically and biochemically in RP 1 and 4 neurons of the African snail, Achatina fulica Ferussac using d- and l-amphetamine (Amp) as modulator. The stereospecific effects, extracellular and intracellular ionic effects, messenger related to enzymatic effects and membrane ionic currents effects on BoP elicited by Amp are studied. The roles of organelles, such as mitochondria, protein syntheis related endoplasmic-reticulum, on the BoP are also tested. The messengers modulated the BoP are discussed. PMID: 18652366 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656750 Sun, 27 Jul 2008 06:42:42 +0100 1656750 http://www.medworm.com/index.php?rid=1656749&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652367%26dopt%3DAbstract Authors: Alania M, Vorontsov DD, Sakharov DA A novel excitatory higher-order input (named Input-HO) to the feeding network of the pond snail, Lymnaea stagnalis, as well as a reliable procedure that makes it possible to initiate and maintain the Input-HO, are described. The Input-HO is primarily targeted to two bilaterally symmetrical pairs of extrabuccal projecting interneurons, the serotonergic Cerebral Giant Cells (CGCs or C1) and FMRFamidergic Pleural-Buccal cells (PlBs). Judging from the results of transection experiments, Input-HO originates in the paired cerebral ganglia where its left and right sources coexist. These sources discharge independently or, sometimes, one after the other. Both inputs project to the ipsilateral CGC/PlB only. In turn, the activity of PlB decreases the ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656749 Sun, 27 Jul 2008 06:42:42 +0100 1656749 http://www.medworm.com/index.php?rid=1656748&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652368%26dopt%3DAbstract Authors: Chistopolsky IA, Vorontsov DD, Sakharov DA To detect neuroactive factors in biological fluids, we have previously elaborated a moveable biosensor, the preparation of isolated neuron penetrated with recording microelectrode. Here, we report that, in the pond snail, signals emanated from a central pattern generator (CPG) for feeding and detected by such biosensor correspond to long-term changes in motor output of the CPG as recorded from buccal nerves. The results suggest that combined recording from motor nerves, neurons in situ and the neuron-based biosensors may be a useful tool in studies of the mechanisms underlying network organisation. PMID: 18652368 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656748 Sun, 27 Jul 2008 06:42:42 +0100 1656748 http://www.medworm.com/index.php?rid=1656747&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652369%26dopt%3DAbstract Authors: Dyakonova TL, Dyakonova VE NO-generating drugs (SNP, SNAP, NaNO2) activated the standard buccal rhythm and simultaneously slowed down the endogenous rhythm of the NO-synthesizing neuron B2 which enhances gut motility. In the B2, these drugs caused giant hyperpolarizing waves strongly associated with the buccal cycles. Hyperpolarizing waves on the B2 could also be induced by prolonged stimulation of the B2. The inhibitor of NO-synthase L-NNA or the NO scavanger PTIO abolished giant hyperpolarizing waves, depolarised the B2 and activated fast bursting in the B2. The results suggest that NO may be involved in coordination of radula and foregut movements. PMID: 18652369 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656747 Sun, 27 Jul 2008 06:42:42 +0100 1656747 http://www.medworm.com/index.php?rid=1656746&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652370%26dopt%3DAbstract Authors: Hernádi L, Kárpáti L, Gyori J, Vehovszky A, Hiripi L We investigated the effect of elevated levels of humoral 5HT and DA on the feeding latency of Helix pomatia, 1 day, 3 days and 10 days following satiation, by injecting monoamines into the haemocoel. HPLC assay of monoamines showed that both 5HT and DA are present in pmol/ml concentrations in the haemolymph of both starved and non-starved animals. Elevated levels of 5HT and DA were most effective at decreasing the feeding latency 10 days following satiation when DA decreased the feeding latency in a concentration dependent manner between 10(-7) and 10(-5) M whereas 5HT levels decreased the feeding latency only at 10(-6) M but increased it at 10(-5) M. Immunocytochemistry revealed that both 5HT3 and D1 receptor... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656746 Sun, 27 Jul 2008 06:42:42 +0100 1656746 http://www.medworm.com/index.php?rid=1656745&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652371%26dopt%3DAbstract Authors: Hernádi L, Hiripi L, Gyori J, Szabó H, Vehovszky A The osmotic stimulation which is able to change the behavioral state of the animal are most effective during rainy weather while they are less effective during dry weather conditions. In isolated CNS preparations from aestivated animals the highest firing activity and serotonin sensitivity of the serotonergic (RPas) heart modulator neurons are recorded during rainy weather and the lowest parameters are observed in dry conditions. In aestivated animals the serotonin (5HT) content in both the CNS and the foot is higher than the dopamine (DA) content during rainy weather, while in dry weather the DA level is higher than the 5HT. The inactivation-reactivation process is accompanied by decreasing both the DA and 5HT level... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656745 Sun, 27 Jul 2008 06:42:42 +0100 1656745 http://www.medworm.com/index.php?rid=1656744&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652372%26dopt%3DAbstract Authors: Balog G, Elekes K Organization of the innervation of the buccal region by 5-HT-immunoreactive (IR) elements was investigated in the pond snail, Lymnaea stagnalis, with special attention to developmental aspects. A gradual maturation is characteristic for the 5-HT-IR muscle innervation, appearing first by late (E80-90%) embryogenesis. It runs parallel with the muscle development and the maturation of the 5-HTergic innervation in the buccal ganglia, peaking by the mid-postembryogenesis (P3) with the presence of a 5-HT-IR network in the buccal mass and rich innervation in the buccal ganglia, including axo-somatic contacts. The whole process seems to match with the appearance of the adult-like feeding (radula protrusion). PMID: 18652372 [PubMed - in process] (Source: Acta Biol... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656744 Sun, 27 Jul 2008 06:42:42 +0100 1656744 http://www.medworm.com/index.php?rid=1656743&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652373%26dopt%3DAbstract Authors: Sadamoto H, Serfozo Z, Ito E The serotonin transporter, SERT, is reported as a key molecule that regulates serotonergic neurotransmission. In the present study, we analyzed the localization of Lymnaea SERT (LymSERT) mRNA-containing neurons by in situ hybridization using frozen sections of the central nervous system (CNS) of Lymnaea. To precisely demonstrate the distribution of LymSERT mRNA-containing neurons, colocalization with serotonin immunoreactivity was also examined. The results showed that LymSERT mRNA was constitutively expressed and localized in the serotonin-containing neurons in the CNS. PMID: 18652373 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656743 Sun, 27 Jul 2008 06:42:42 +0100 1656743 http://www.medworm.com/index.php?rid=1656742&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652374%26dopt%3DAbstract Authors: Kurokawa M, Ito S, Okamoto T In order to explore the functions of the peripheral neurons in the enteric nervous system (ENS) of the gastropods, Aplysia and Lymnaea, we investigated the correlation between peripheral neuronal activities and movements of the digestive tract. In Aplysia, movements of the gizzard were distinguished into two types of contraction: a large constriction of the whole gizzard following bursting activities of the neurons on the gizzard and EJP-like potentials in the musculature; and a small contraction of a restricted part of the gizzard following a slow muscle potential. When TTX was applied to isolated gizzard preparation, the bursting activities were blocked and the EJP-like potentials and the subsequent constriction disappeared, whereas the slow pote... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656742 Sun, 27 Jul 2008 06:42:42 +0100 1656742 http://www.medworm.com/index.php?rid=1656741&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652375%26dopt%3DAbstract Authors: Matsuo R, Ito E The procerebrum (PC) is indispensable for odor-aversion learning in Limax. On the other hand, the central nervous system (CNS) of some Pulmonata shows robustness against injury, recovering from nerve injury both at the histological and functional levels. To investigate whether the PC of Limax also shows robustness against nerve injury, we tested whether or not the slugs can acquire and retrieve odor-aversion memory after a long recovery period from PC ablation. When the recovery period is short (7 days), the PC-ablated slugs failed to avoid the conditioned odor. But when the recovery period is long (1 month), the PC-ablated slugs successfully avoided the conditioned odor. These results indicate that the CNS including the PC can recover from injury at least at t... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656741 Sun, 27 Jul 2008 06:42:42 +0100 1656741 http://www.medworm.com/index.php?rid=1656740&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652376%26dopt%3DAbstract Authors: Kobayashi S, Hattori M, Ito E Gamma-aminobutyric acid (GABA)-containing neurons are capable of controlling network oscillations and their patterns in the widely divergent species. In the terrestrial slug, Limax valentianus, oscillatory activity of the procerebrum neurons is considered to encode the odor information. Previous studies showed that GABA is present in the central nervous system and may be involved in the control of neuronal activity of Limax. In the present study, we examined the effects of GABA on the generation of oscillatory activity in the procerebrum. The results suggest that the GABAergic synaptic transmission may be involved in the oscillatory neural network of the procerebrum. PMID: 18652376 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656740 Sun, 27 Jul 2008 06:42:42 +0100 1656740 http://www.medworm.com/index.php?rid=1656739&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652377%26dopt%3DAbstract Authors: Nomura Y, Hatakeyama D, Horikoshi T, Sakakibara M Behavioral conditioning in Lymnaea increased the amount of immunolabeling in the central nervous system for the memory-associated protein calexcitin. The staining level of anti-calexcitin positive neurons was always stronger in conditioned animals than in naive animals. In the visuo-vestibular conditioned animals, right-parietal and visceral group neurons as well as withdrawal-related neurons were positively stained with anti-calexcitin antibody. In taste-aversion conditioned animals, right-parietal visceral G-group neurons and withdrawal-related neurons were selectively stained. These neurons are candidate neurons for modulation by these conditioning paradigms. PMID: 18652377 [PubMed - in process] (Source: Acta Biologica H... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656739 Sun, 27 Jul 2008 06:42:42 +0100 1656739 http://www.medworm.com/index.php?rid=1656738&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652378%26dopt%3DAbstract Authors: Suzuki H, Horikoshi T, Sakakibara M Lymnaea are capable of learning an association between light and rotation. The conditioning paradigm to produce this association and the unconditioned escape response are the same for both Lymnaea and Hermissenda, but the underlying neural mechanisms appear to differ between the two species due to differences in the organization and function of their visuo- and vestibular-sensory systems. The RPeD11 interneuron in Lymnaea controls withdrawal behavior. We analyzed the electrophysiologic characteristics of the RPeD11 and observed an increase in cell excitability; that is, spontaneous activity of the postsynaptic potential in the RpeD11 was increased in conditioned animals after learning acquisition. PMID: 18652378 [PubMed - in process] (So... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656738 Sun, 27 Jul 2008 06:42:42 +0100 1656738 http://www.medworm.com/index.php?rid=1656737&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652379%26dopt%3DAbstract Authors: Karasawa T, Sato N, Horikoshi T, Sakakibara M Though adult Lymnaea are bimodal breathers, young animals breathe mainly through the skin and adults through the lung. Operant conditioning changes adult breathing behavior from aerial to cutaneous. We hypothesized that this behavioral change is caused by alterations in the neuronal circuit during both development and conditioning. We focused our study on whether the synaptic connection between RPeD1 and RPA6 neurons is modulated during development and conditioning. Our findings indicated that the RPeD1 has an excitatory monosynaptic contact with the RPA6 in young naive and operantly-conditioned adult animals. The relationship of this contact was well correlated with their respiratory behavior. PMID: 18652379 [PubMed - in proce... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656737 Sun, 27 Jul 2008 06:42:42 +0100 1656737 http://www.medworm.com/index.php?rid=1656736&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652380%26dopt%3DAbstract Authors: Tsyganov VV In a reduced preparation of Planorbarius corneus consisting of the CNS and mantle complex, both the dopamine precursor L-DOPA and the serotonin precursor 5-HTP have been found to be able to induce and maintain rhythmic pneumostome (PN) movements coupled, in a neurotransmitter-specific manner, to fictive cyclic locomotion. Following transection of the pedal commissures, the pharmacologically induced movements of the PN were coordinated with the locomotory rhythm generated by the left pedal ganglion. PMID: 18652380 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656736 Sun, 27 Jul 2008 06:42:42 +0100 1656736 http://www.medworm.com/index.php?rid=1656735&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652381%26dopt%3DAbstract Authors: Aono K, Fusada A, Fusada Y, Ishii W, Kanaya Y, Komuro M, Matsui K, Meguro S, Miyamae A, Miyamae Y, Murata A, Narita S, Nozaka H, Saito W, Watanabe A, Nishikata K, Kanazawa A, Fujito Y, Okada R, Lukowiak K, Ito E The pond snail, Lymnaea stagnalis, can locomote on its back utilizing the surface tension of the water. We have called this form of movement 'back-swimming'. In order to perform this behavior, the snail must flip itself over on its back so that its foot is visible from above. Little is known about the mechanism of this back-swimming. As a first step for the elucidation of this mechanism, we measured the speed of back-swimming of Lymnaea at the different times of the day. They back-swam significantly faster in the morning than just before dark. These data are consistent... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656735 Sun, 27 Jul 2008 06:42:42 +0100 1656735 http://www.medworm.com/index.php?rid=1656734&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652382%26dopt%3DAbstract This study presents preliminary results on the distribution of FMRFamide-like immunoreactive neurons within the CNS of the pygmy squid Idiosepius notoides. Its gross neuroanatomy resembles that of other cephalopods. FMRFamide-like immunoreactivity was observed in most of the brain lobes. High abundance of FMRFamidergic perikarya was found in the dorsal basal, the central palliovisceral, and the olfactory lobes, whereas none were observed in the middle suboesophageal mass. Single individual perikarya are located within the optic lobes and the vertical lobes. Although certain immunohistochemical traits are shared with other cephalopods, such as a wall-like arrangement of FMRFamide-like immunoreactive cell somata within the dorsal basal lobe, others have so far only been found in Idiosepius. ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656734 Sun, 27 Jul 2008 06:42:42 +0100 1656734 http://www.medworm.com/index.php?rid=1656733&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652383%26dopt%3DAbstract Authors: Voronezhskaya EE, Glebov KI, Khabarova MY, Ponimaskin EG, Nezlin LP The regulation of larval development by starved adults occurs in both freshwater snails, Helisoma trivolvis and marine polychaetes, Platynereis dumerilii. Serotonin (5-HT) links this environmental signal which is detected by larval apical sensory neurons to changes in larval development. A profile of the stage-dependent expression of 5-HT receptors and coupled G proteins is essential in this regulatory mechanism. The final effect on development depends on the modulation of the activity of the larval digestive system. PMID: 18652383 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656733 Sun, 27 Jul 2008 06:42:42 +0100 1656733 http://www.medworm.com/index.php?rid=1656732&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652384%26dopt%3DAbstract Authors: Chaban AK, Voronezhskaya EE The role of transient larval FMRFa-ergic neurons of the freshwater snails, Helisoma trivolvis and Lymnaea stagnalis, in osmoregulation and neurodifferentiation was investigated. It was shown that: (i) FMRFa and an FMRFa antibody do not reach their targets when injected into the egg capsule; (ii) long-term incubations of the embryos in neomycin and hyperosmotic solutions of sea water, NaCl and sucrose during the definite developmental stages lead to a special malformation-hydropia; (iii) hydropia coincides with an extensive larval FMRFa-ergic nervous system; (iv) the increased level of FMRFa causes earlier serotonin synthesis by the neurons of the visceral loop ganglia. PMID: 18652384 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656732 Sun, 27 Jul 2008 06:42:42 +0100 1656732 http://www.medworm.com/index.php?rid=1656731&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652385%26dopt%3DAbstract Authors: Wanninger A Recently, a number of neurodevelopmental studies of hitherto neglected taxa have become available, contributing to questions relating to the evolution of the nervous system of Lophotrochozoa (Spiralia + Lophophorata). As an example, neurogenesis of echiurans showed that these worm-shaped spiralians, which as adults do not exhibit any signs of segmentation, do show such traits during ontogeny, e.g. by segmentally arranged perikarya and commissures. Similarly, sipunculan worms, which have a single ventral nerve cord in the adult stage, develop this nerve cord by gradual fusion of a paired larval nerve during metamorphosis, and show transitional stages of segmentation. These findings indicate that echiurans, annelids and sipunculans stem from a segmented ancestor. By ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656731 Sun, 27 Jul 2008 06:42:42 +0100 1656731 http://www.medworm.com/index.php?rid=1656730&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652386%26dopt%3DAbstract Authors: Kumashiro M, Iwano M, Sakai M The genitalia of the male cricket, Gryllus bimaculatus, is automatically maintained. It is carried out by spontaneous undulation of the scaled membrane consisting of the genital chamber floor. To understand the mechanism of that movement, part of the membrane was cut out and examined in vitro with biogenic amines, and the spike activity of neurons innervating muscle fibers of the membrane was analyzed. The esults indicated that the fragment of the membrane, which showed spontaneous twitching in saline, increased its frequency at 5-HT application. In contrast, mianserin (5-HT antagonist) decreased its occurence. Immunocytochemical study indicated that massive 5-HT-positive branchibgs of one main axon nnervated muscle fibers of the genital membrane.... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656730 Sun, 27 Jul 2008 06:42:42 +0100 1656730 http://www.medworm.com/index.php?rid=1656729&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652387%26dopt%3DAbstract Authors: Tomioka K, Miyasako Y, Umezaki Y Drosophila shows bimodal circadian locomotor rhythms with peaks around light-on (morning peak) and before light-off (evening peak). The rhythm synchronizes to light and temperature cycles and the synchronization is achieved by two sets of clocks: one entrains to light cycles and the other to temperature cycles. The light-entrainable clock consists of the clock neurons located in the lateral protocerebrum (LNs) and the temperature-entrainable clock involves those located in the dorsal protocerebrum (DNs) and the cells located in the posterior lateral protocerebrum (LPNs). To understand the interaction between the light-entrainable and the temperature-entrainable clock neurons, locomotor rhythms of the mutant flies lacking PDF or PDF-positive clo... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656729 Sun, 27 Jul 2008 06:42:42 +0100 1656729 http://www.medworm.com/index.php?rid=1656728&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652388%26dopt%3DAbstract Authors: Okada R, Ikeno H, Sasayama N, Aonuma H, Kurabayashi D, Ito E A honeybee informs her nestmates of the location of a flower she has visited by a unique behavior called a "waggle dance." On a vertical comb, the direction of the waggle run relative to gravity indicates the direction to the food source relative to the sun in the field, and the duration of the waggle run indicates the distance to the food source. To determine the detailed biological features of the waggle dance, we observed worker honeybee behavior in the field. Video analysis showed that the bee does not dance in a single or random place in the hive but waggled several times in one place and then several times in another. It also showed that the information of the waggle dance contains a substantial margin of error... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656728 Sun, 27 Jul 2008 06:42:42 +0100 1656728 http://www.medworm.com/index.php?rid=1656727&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652389%26dopt%3DAbstract Authors: Shuranova Z The crayfish, Procambarus cubensis, placed in the central area of a plus-maze preferred to go forward in about 50% of trials; they chose the right or left arm in 20.7 and 18.9% of the trials, respectively. In a T-maze, the difference between right and left directions was also insignificant. When exploring a plus-maze, the crayfish turned to 180 degrees at the end of the arms, then turned to 90 degrees going to the next arm. The mean difference between the right and left U-turns, and the right and left turns was insignificant though some animals demonstrated a left or right preference. There was a strong correlation between the direction of U-turns and following turns ensuring the clockwise or anti-clockwise movements of the crayfish. Also we examined a possible pre... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656727 Sun, 27 Jul 2008 06:42:42 +0100 1656727 http://www.medworm.com/index.php?rid=1656726&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652390%26dopt%3DAbstract Authors: Schürmann FW, Frambach I, Elekes K Distribution and synaptic connections of GABA fibres in neuropil parts of the mushroom bodies in brains of crickets (Gryllus bimaculatus) and bees (Apis mellifera) were investigated by immuno-light and electron microscopy. In the inner calyx neuropil of cricket mushroom bodies, GABA fibres are pre- and post-synaptically connected with proximal Kenyon cell dendrites, indicating synaptic contacts differing from those of the Kenyon cell dendritic tips in the peripheral microglomeruli of the calyces. A more complex interaction of GABAergic fibres and Kenyon cell dendrites than assumed before is shown. In the mushroom bodies of bees, dendritic like strata of GABA fibre projections contribute to the subcompartmental layers of the vertical lobe... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656726 Sun, 27 Jul 2008 06:42:42 +0100 1656726 http://www.medworm.com/index.php?rid=1656725&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652391%26dopt%3DAbstract Authors: Sakura M, Hiraguchi T, Ohkawara K, Aonuma H Pheromones are important cues for social insects such as ants. As a first step in elucidation of pheromonal information processing mechanisms in the myrmicine ant, we investigated the morphological structure of the antennal lobe. Using autofluorescence imaging, labeling of neuronal filamentous actin, and reduced silver impregnation staining, the antennal lobe was found to consist of five compartments that, each received input from a different antennal sensory tract. Two major tracts of projection neurons, the medial and lateral antenno-cerebral tract (m- and 1-ACT), originated from a different region of the antennal lobe. The m-ACT originated from the posterior part of the antennal lobe whereas the 1-ACT originated from the anterior ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656725 Sun, 27 Jul 2008 06:42:42 +0100 1656725 http://www.medworm.com/index.php?rid=1656724&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652392%26dopt%3DAbstract Authors: Papaioannou S, Holden-Dye L, Walker RJ The actions of six Neuropeptide-like Peptides (NLPs) were investigated on Ascaris suum dorsal body wall muscle and Caenorhabditis elegans pharyngeal muscle. The NLPs tested on A. suum muscle, viz, NLP-1A, NLP-2A and B, had no direct effect on the muscle but all reversibly reduced acetylcholine (ACh)-induced contractions of the muscle, NLP-2A being the most potent, with an IC50 value of 78 microM. NLP-2A also reversibly reduced ACh-induced depolarizations of A. suum muscle resting membrane potential. NLP-1A, NLP-2A, NLP-3C and NLP-10A all increased pharyngeal pumping in C. elegans, NLP-8A weakly inhibited pumping while NLP-2B was inactive. NLP-10A induced a concentration-dependent increase in pumping activity with a threshold of 10-100 nM.... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656724 Sun, 27 Jul 2008 06:42:42 +0100 1656724 http://www.medworm.com/index.php?rid=1656723&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652393%26dopt%3DAbstract Authors: Kreshchenko ND The development of the new pharynx in anterior body fragments of G. tigrina was followed for 7 days by immunocytochemical (ICC) study using antiserum to neuropeptide F (NPF) and muscle staining with phalloidin. ICC investigation revealed the presence of NPF in pharyngeal nervous system, peripheral nerve plexuses, in central nervous system of intact planarians. NPF-immunoreactive (IR) nerve fibres were found at the site of regeneration surrounding pharyngeal rudiment. Restoration of the pharynx function during regeneration was analysed by appearance of food response in anterior fragments. Stimulating effects of NPF and FMRF on the pharyngeal regeneration has been observed. The data indicates an important role of neuropeptides in morphogenetic processes. PMID:... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656723 Sun, 27 Jul 2008 06:42:42 +0100 1656723 http://www.medworm.com/index.php?rid=1656722&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652394%26dopt%3DAbstract Authors: Biserova NM It is still unclear whether flatworms have specialized glial cells. At present there are no special methods available for the identification of glial cells in flatworms. The aim of this research was to carry out detailed investigations of the CNS in two species ofcestodes, and to get an idea whether these cells may fit into the concept of glia. Three types of glial cells have been found in Grillotia erinaceus: (1) fibroblast-like cells in the cerebral ganglion (CG); (2) glial cells in bulbar nerves with filaments and laminar cytoplasm; (3) a 3rd type of cells forms multilayer envelopes in the main cords (MC); also they make contacts with the excretory epithelium. To demonstrate the existence of glial cells, an immunocytochemical and ultrastructural investigation of... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656722 Sun, 27 Jul 2008 06:42:42 +0100 1656722 http://www.medworm.com/index.php?rid=1656721&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652395%26dopt%3DAbstract Authors: Tolstenkov OO, Terenina N, Gustafsson M, Serbina E, Kreshchenko ND, Maklakova L, Jashina A Serotoninergic and FMRFamidergic components of the nervous system were examined in cercaria from different types using immunocytochemical techniques interfaced with confocal scanning laser microscopy. Cercariae from 9 families were studied - Opisthorchis felineus, Parafasciolopsis fasciolaemorpha, Echinochasmus coaxatus, Echinoparyphium aconiatum, Notocotylus attenuatus, Psilotrema tuberculata, Plagiorchis sp., Cyathocotyle bithyniae, Diplostomum chromatophorum. The results show that 5-HT-IR and FMRFamide-IR occur in all types of cercariae, regardless of what morphological, taxonomic and biological group they belong to. Small differences in the patterns of 5-HT-IR and FMRFamide-IR were o... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656721 Sun, 27 Jul 2008 06:42:42 +0100 1656721 http://www.medworm.com/index.php?rid=1656720&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652396%26dopt%3DAbstract Authors: Takahashi T, Hayakawa E, Koizumi O, Fujisawa T In order to identify novel peptide signaling molecules involved in the regulation of developmental and physiological processes in the freshwater cnidarian, Hydra magnipapillata, we initiated a systematic peptide screening project, the Hydra Peptide Project. In the project, twelve neuropeptides were identified so far. The LWamide family is composed of seven members, which share a GLWamide motif at their C-termini. All the peptides have an ability to induce metamorphosis of Hydractinia serrata planula larvae into polyps. In Hydra, LWamides induce detachment of the bud from a parental polyp. A neuropeptide, Hym-355, enhances neuronal differentiation by inducing the multipotent interstitial stem cells to enter the neuron differentiati... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656720 Sun, 27 Jul 2008 06:42:42 +0100 1656720 http://www.medworm.com/index.php?rid=1656719&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652397%26dopt%3DAbstract In this study, we investigated a novel function of TK as an enhancer of oocyte growth. Ci-TK-R is expressed specifically in the Ciona vitellogenic oocytes. Moreover, administration of Ci-TK to the Ciona ovary resulted in upregulation of gene expression and enzymatic activity of several proteases. Moreover, maturation of the Ciona oocytes from the vitellogenic stage to the post-vitellogenic stage was induced in the presence of Ci-TK, which was completely blocked by addition of protease inhibitors. PMID: 18652397 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656719 Sun, 27 Jul 2008 06:42:42 +0100 1656719 http://www.medworm.com/index.php?rid=1656718&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18652398%26dopt%3DAbstract In this study, we show the functional modulation of Ci-GnRHR-1 via heterodimerization with the orphan receptor subtype, Ci-GnRHR-4. The dimerization between Ci-GnRHR-1 and R-4 was detected by co-immunoprecipitation and immunoblot analysis. Binding assays confirmed the binding of t-GnRHs to Ci-GnRHR-1 but not to R-4, and verified no alternation in ligand-binding affinity between Ci-GnRHR-1 homodimer and Ci-GnRHRI&4 heterodimer. The heterodimer was found to stimulate the elevation of intracellular calcium, time-extension of ERK phosphorylation, and up-regulation of cell proliferation, all in a ligand specific manner, compared with the Ci-GnRHR-1 homodimer. In combination, these results indicated that Ci-GnRHR-4 is not an inactive receptor, but a modulatory factor for Ci-GnRHR-1 in C. int... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1656718 Sun, 27 Jul 2008 06:42:42 +0100 1656718 http://www.medworm.com/index.php?rid=1644511&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637554%26dopt%3DAbstract Authors: Kelemen O, Hegedus G, Kollár L, Menyhei G, Seress L The linear hypertrophic scar has become the most common type of pathologic scarring. Silicone-gel sheeting is the first line therapy while intralesional steroid is the second. A light and electron microscopic analysis was carried out to reveal differences in tissue reaction following the two different treatments. Two groups of 12 patients each were treated for 4 months. For the first group, diluted Triamcinolone acetonide was injected until an inactive state was achieved. The other group of patients was treated with silicone-gel sheeting. The scars were examined every two weeks and their appearance documented. After reaching the expected therapeutic response, inactive scars were removed. The excised scars were evaluated ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644511 Sun, 01 Jun 2008 04:00:00 +0100 1644511 http://www.medworm.com/index.php?rid=1644510&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637555%26dopt%3DAbstract Authors: Takács J, Zaninetti R, Vig J, Vastagh C, Hámori J We have investigated the expression of Doublecortin (Dcx) protein in the developing cerebellum of mouse from postnatal 2nd day to postnatal 22nd day and in young adults by immunohistochemistry. Strong expression of Dcx was present in the inner zone of the external granule cell layer, and remained strong while postmitotic granule cell precursors were present in this transitory layer. Descending granule cell precursors exhibited Dcx immunostaining not only while migrating but for a short time also after their settlement. Dcx-immunostained cells appeared in deep cerebellocortical territories and in the cerebellar white matter during the first postnatal week. These bipolar cells were arranged in the sagittal plane and bui... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644510 Sun, 01 Jun 2008 04:00:00 +0100 1644510 http://www.medworm.com/index.php?rid=1644509&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637556%26dopt%3DAbstract Authors: Mogulkoc R, Baltaci AK The present study was conducted to determine the effects of basal, isotonic as well as hypertonic and hypovolemic treatments on fluid-electrolyte balance and plasma AVP levels in rats supplemented with L-thyroxine and pinealectomized L-thyroxine. The animals were initially separated into 4 groups: control (n = 24), L-thyroxine treated (n = 24); L-thyroxine + sham-pinealectomy (n = 24) treated and 4-L-thyroxine + pinealectomy (n = 24) treated. L-thyroxine was given for 4 weeks. At the end of the 4-week experimental period, the sub-groups were formed before decapitation, which were classified as unstimulated (n = 6), isotonic (n = 6), hypertonic (n = 6) and hypovolemic (n = 6) stimulation. Plasma AVP, total triiodothyronine (TT3) and total thyroxine (TT4) ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644509 Sun, 01 Jun 2008 04:00:00 +0100 1644509 http://www.medworm.com/index.php?rid=1644508&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637557%26dopt%3DAbstract Authors: Kaminska B, Opalka M, Dusza L The aim of this study was to investigate the effects of ACTH, phytoestrogens (genistein, daidzein, biochanin A and coumestrol), and animal estrogens (estradiol and estrone) on corticosterone secretion by isolated adrenocortical cells of the ganders in breeding (April) and nonbreeding seasons (July). ACTH stimulated corticosterone output in the breeding season. In July (photorefractoriness and postbreeding molt) ACTH had no effect on corticosterone production. Coumestrol reduced corticosterone secretion by the cells obtained in nonbreeding season. Other examined phytoestrogens did not affect corticosterone production. Estrogens showed differentiated effects. Estradiol stimulated the corticosterone output in breeding season; estrone inhibited cortic... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644508 Sun, 01 Jun 2008 04:00:00 +0100 1644508 http://www.medworm.com/index.php?rid=1644507&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637558%26dopt%3DAbstract This study examines fatty acid profiles and susceptibility to lipid peroxidation in liver and heart mitochondria obtained from Adelie penguin (Pygoscelis adeliae). The saturated fatty acids in these organelles represent approximately 40-50% of total fatty acids whereas the polyunsaturated fatty acid composition was highly distinctive, characterized by almost equal amounts of 18:2 n-6; 20:4 n-6 and 22:6 n-3 in liver mitochondria, and a higher proportion of 18:2 n-6 compared to 20:4 n-6 and 22:6 n-3 in heart mitochondria. The concentration of total unsaturated fatty acids of liver and heart mitochondria was approximately 50% and 60%, respectively, with a prevalence of oleic acid C18:1 n9. The rate C20:4 n6/C18:2 n6 and the unsaturation index was similar in liver and heart mitochondria; 104.3... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644507 Sun, 01 Jun 2008 04:00:00 +0100 1644507 http://www.medworm.com/index.php?rid=1644506&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637559%26dopt%3DAbstract In this study molecular markers linked to the Rysto gene, which originates from the wild potato species Solanum stoloniferum and confers extreme resistance against PVY, were identified and the applicability of recently published Rysto, markers was analyzed. Three RAPD markers covering a total distance of 8.60 cM were detected in this experiment. The closest of these markers was located 0.53 cM from the gene. From among the published markers only one had diagnostic value in the experimental plant material, and mapped 2.95 cM from the gene, on the side opposite the RAPD markers developed in the present study. All the markers analyzed were present in Solanum stoloniferum accessions, irrespective of their resistance, indicating that these sequences are linked to the locus and not exclusively t... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644506 Sun, 01 Jun 2008 04:00:00 +0100 1644506 http://www.medworm.com/index.php?rid=1644505&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637560%26dopt%3DAbstract Authors: Karsai I, Koszegi B, Kovács G, Szucs P, Mészáros K, Bedo Z, Veisz O In order to analyse the effects of temperature (9-22 degreesC) and light intensity (170-576 micromol m(-2) s(-1)) on plant development two barley varieties with contrasting seasonal growth habits were included in a series of experiments consisting of controlled environment tests. The effect of constant (18 degrees C) and daily fluctuating (18/16 degrees C) temperature with a long photoperiod was also examined in a set of barley varieties including winter, facultative and spring barleys. Dicktoo with facultative growth habit was more sensitive to unfavourable conditions than Kompolti korai with winter growth habit; the flowering of Dicktoo was significantly delayed by sub- and supra-optimal tempe... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644505 Sun, 01 Jun 2008 04:00:00 +0100 1644505 http://www.medworm.com/index.php?rid=1644504&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637561%26dopt%3DAbstract Authors: Lepedus H, Fulgosi H, Bensić M, Cesar V The photosynthetic performance of developing spruce (Picea abies L. Karst.) needles was investigated. As revealed by previous reports, the biosynthesis of chlorophylls and carotenoids was not following the characteristic chloroplast ultrastructure building up during needle elongation process. The aim of our study was to investigate photosynthetic capability (evaluated by oxygen evolution and chlorophyll a fluorescence kinetics measurements), the dynamics of chloroplast pigments biosynthesis and the expression of major photosynthetic proteins as well as to find out possible correlation between components of issue. Low amounts of chlorophylls and carotenoids, LHC II and Rubisco LSU were detected in the embryonic shoot of vegetative b... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644504 Sun, 01 Jun 2008 04:00:00 +0100 1644504 http://www.medworm.com/index.php?rid=1644503&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637562%26dopt%3DAbstract Authors: Bouazizi H, Jouili H, Geitmann A, El Ferjani E We studied oxidative stress and peroxidase activity resulting from application of excess copper in the nutrient medium on the roots of young bean seedlings. The change in H2O2 content, lipid peroxidation and antioxidant enzymes activities were quantified and located. Excess of copper caused a loss of membrane integrity and the formation of hydrogen peroxide (H2O2) as visualized in the transmission electron microscopy and measured using spectrophotometry. H2O2 accumulated in the intercellular spaces and in the cell wall. The production of H2O2 was accompanied by an increase in the activity of soluble and ionic GPX (guaiacol peroxidase, EC 1.11.17), CAPX (coniferyl alcohol peroxidase) and NADH oxidase. PMID: 18637562 [PubMed - i... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644503 Sun, 01 Jun 2008 04:00:00 +0100 1644503 http://www.medworm.com/index.php?rid=1644502&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637563%26dopt%3DAbstract Authors: Masood A, Zeeshan M, Abraham G Effect of ultravilolet-B (0.4 Wm(-2)) irradiation on growth, flavonoid content, lipid peroxidation, proline accumulation and activities of superoxide dismutase and peroxidase was comparatively analysed in Azolla pinnata and Azolla filiculoides. Growth measured as increment in dry weight reduced considerably due to all UV-B treatments. However, the reduction was found to be severe in A. filiculoides as compared to A. pinnata. The level of UV-absorbing compound flavonoids increased significantly in A. pinnata plants whereas only a slight increase in the flavonoid content was observed in A. filiculoides. UV-B exposure led to enhanced production of malondialdehyde (MDA) and electrolyte leakage in A. filiculoides than A. pinnata. Proline accumulation ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644502 Sun, 01 Jun 2008 04:00:00 +0100 1644502 http://www.medworm.com/index.php?rid=1644501&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18637564%26dopt%3DAbstract Authors: Kredics L, Terecskei K, Antal Z, Szekeres A, Hatvani L, Manczinger L, Vágvölgyi C Eleven cold-tolerant Trichoderma isolates were screened for the production of proteolytic activities at 10 degrees C. Based on the activity profiles determined with paranitroanilide substrates at 5 degrees C, strain T221 identified as Trichoderma atroviride was selected for further investigations. The culture broth of the strain grown at 10 degrees C in casein-containing culture medium was concentrated by lyophilization and subjected to gel filtration, which was followed by chromatofocusing of the fraction showing the highest activity on N-benzoyl-Phe-Val-Arg-paranitroanilide. The purified enzyme had a molecular weight of 24 kDa, an isoelectric point of 7.3 and a pH optimum of 6.2. The ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1644501 Sun, 01 Jun 2008 04:00:00 +0100 1644501 http://www.medworm.com/index.php?rid=1368191&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401941%26dopt%3DAbstract Authors: Singh UP, Krishna A, Bhatnagar KP Detailed reproductive pattern and associated endocrine characteristics have been documented in only a few species of order Chiroptera. The aim of the present study was to examine the changes in body weight, serum insulin, leptin, androstenedione and luteinizing hormone (LH) concentrations during annual ovarian cycle in the sheath-tailed bat, Taphozous longimanus. Bats were sampled over three years. Leptin, a satiety hormone produced primarily by adipose tissue, provides information to feeding center of the brain about nutritional status, fat mass, appetite and energy expenditure. The circulating concentration of leptin begins to increase from October and attains a peak in December. The peak serum leptin concentration coincides with body weight... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368191 Sat, 01 Mar 2008 05:00:00 +0100 1368191 http://www.medworm.com/index.php?rid=1368190&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401942%26dopt%3DAbstract Authors: Koricanac G, Stojiljkovic M, Radivojsa S, Zakula Z, Ribarac-Stepic N, Isenovic ER The aim of this study was to examine the effects of dexamethasone (Dex) on functional properties of the rat insulin receptor (IR). Male Mill Hill hooded rats, 3, 6, 12, 18 and 21 months old, were injected with Dex (4 mg/kg) and rat liver and erythrocytes were used for experiments 18 h after Dex administration. Treatment with Dex lowered the specific binding (SB) of insulin (INS) in the liver of 3- and 18-month-old rats and concentration of INS binding sites (N1, N2) and the dissociation constant of low-affinity binding sites (Kd2) in the liver of 6- and 18-month-old rats. In addition, Dex treatment lowered the liver IR protein level in all analyzed groups, except 21-month-old rats where it remain... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368190 Sat, 01 Mar 2008 05:00:00 +0100 1368190 http://www.medworm.com/index.php?rid=1368189&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401943%26dopt%3DAbstract Authors: Zawisza-Raszka A, Dolezych B The effect of single and combined action of nickel and pesticide (diazinon) on enzymes activity (glutathione S-transferase, catalase and acetylcholinesterase) in the digestive tract, body wall and fat body as well as basic growth parameters (life span and body mass) of Spodoptera exigua were investigated under laboratory conditions. The experiment was carried out on two nickel treated groups [300 (NiI) and 900 (NiII) mg Ni kg(-1) dry weight of the culture medium] and a control group. The results showed that mortality of caterpillars in NiII group was higher (51.1%) when compare with the controls. The body mass of the caterpillars in the NiI group was higher by 20% than in the control group, and the body mass of the pupae in the NiII group was lower... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368189 Sat, 01 Mar 2008 05:00:00 +0100 1368189 http://www.medworm.com/index.php?rid=1368188&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401944%26dopt%3DAbstract Authors: Stamenkovic-Radak M, Kalajdzic P, Savic T, Savic M, Kurbalija Z, Rasic G, Andjelkovic M We analyzed the developmental time, egg-to-adult viability, and developmental stability (fluctuating wing size asymmetry) in Drosophila subobscura, maintained for six generations on different concentrations of lead. Development time is significantly affected by generation and lead concentration, but interaction of these factors is not a significant source of variability for this fitness component. Generation and the interaction generation x concentration of lead significantly affect egg-to-adult viability. Levene's test of heterogeneity of variance showed that variability of FA is not significant in any of the samples. Within both lead concentrations females showed significantly higher FA i... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368188 Sat, 01 Mar 2008 05:00:00 +0100 1368188 http://www.medworm.com/index.php?rid=1368187&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401945%26dopt%3DAbstract In this study, we found that exposure of extended (48 h) cold (8 degrees C) caused changes both in the antioxidant defense system (as tissue and enzyme specific) and serum lipoprotein profiles in rats. PMID: 18401945 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368187 Sat, 01 Mar 2008 05:00:00 +0100 1368187 http://www.medworm.com/index.php?rid=1368186&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401946%26dopt%3DAbstract Authors: Pavicic I, Trosic I The aim of this study was to evaluate and compare the influence of 864 MHz and 935 MHz radiofrequency/microwave (RF/MW) fields on the growth, colony-forming ability, and viability of V79 cells (continuous line). Cell samples with 1 x 10(4) V79 cells each, were exposed to continuous wave frequencies of 864 MHz and 935 MHz for 1, 2 and 3 hours. Exposed samples were matched with unexposed control samples. Specific absorption rate (SAR) was 0.08 W/kg for the 864 MHz or 0.12 W/kg for the 935 MHz field. Cell growth and viability were determined by counting cells every day for five days after exposure. Colony-forming ability was assessed by counting colonies seven days after exposure. The growth of the 864 MHz-irradiated cells was significant after two- and three-... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368186 Sat, 01 Mar 2008 05:00:00 +0100 1368186 http://www.medworm.com/index.php?rid=1368185&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401947%26dopt%3DAbstract Authors: Rout GR, Senapati SK, Panda JJ Sodium chloride tolerant organogenic callus lines of Nicotiana tabacum were developed in vitro on Murashige and Skoog [16] medium supplemented with BA, IAA and different concentration of NaCl. The maximum shoot bud regeneration was achieved from both tolerant and non-tolerant calluses on MS medium supplemented with 1.0 mg/l BA, 0.1 mg/l IAA with or without NaCl within 4 weeks of culture. Standard growth parameters such as fresh weight and dry weight of organogenic callus, growth tolerant index and enzyme activity (peroxidase and catalase) were used as indicators of salt tolerance. The growth tolerance index in the 4-week after the beginning of treatments yielded significant differences among the non-tolerant and tolerant organogenic callus lines.... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368185 Sat, 01 Mar 2008 05:00:00 +0100 1368185 http://www.medworm.com/index.php?rid=1368184&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401948%26dopt%3DAbstract Authors: Unal M, Palavan-Unsal N, Tufekci MA The genotoxic and cytotoxic effects of exogenous polyamines (PAs), putrescine (Put), spermidine (Spd), spermine (Spm) and PA biosynthetic inhibitors, alpha-difluoromethylornithine (DFMO), cyclohexilamine (CHA), methylglioxal bis-(guanylhydrazone) (MGBG) were investigated in the root meristems of Allium cepa L. The reduction of mitotic index and the induction of chromosomal aberrations such as bridges, stickiness, c-mitotic anaphases, micronuclei, endoredupliction by PAs and PA biosynthetic inhibitors were observed and these were used as evidence of genotoxicity and cytotoxicity. PMID: 18401948 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368184 Sat, 01 Mar 2008 05:00:00 +0100 1368184 http://www.medworm.com/index.php?rid=1368183&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401949%26dopt%3DAbstract Authors: Sramkó G, Gulyás G, Matus G, Rudnóy S, Illyés Z, Bratek Z, Molnár AV The taxonomy of the genus Bulbocodium, in which two European species, a smaller eastern (B. versicolor) and a more robust western (B. vernum) are included, has been controversial since the description of the eastern species in 1821. Nuclear encoded ribosomal DNA ITS1 and the entire chloroplast DNA ITS were sequenced from several European populations, from France to the Ukraine, and the leaf width of mature living individuals was measured and analysed by ANOVA and Tukey-test. Although the studied DNA regions proved to be invariable, leaf width shows extreme variability. We found no correlation between the leaf size of the individuals and the geographical position of the populations, an... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368183 Sat, 01 Mar 2008 05:00:00 +0100 1368183 http://www.medworm.com/index.php?rid=1368182&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18401950%26dopt%3DAbstract Authors: Wielbo J, Skorupska A The Rhizobium-legume interaction is sensitive to a number of environmental factors, among which phosphate (Pi) and ammonium availability are the most important. We investigated the effect of Pi and ammonia concentration on exopolysaccharide production and symbiosis Trifolium pratense with Rhizobium leguminosarum bv. trifolii TA1 (RtTA1). The optimal Pi concentration in the bacterial growth medium for RtTA1 growth and exopolysaccharide production was in the range from 0.9 mM to 8.1 mM. Independently of Pi concentration, ammonium (NH4Cl) concentration above 8.1 mM in the culture medium significantly decreased EPS production, indicating a regulatory role of this nutrient on the EPS production in the RtTA1 strain. Pi availability has a beneficial effect on bo... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1368182 Sat, 01 Mar 2008 05:00:00 +0100 1368182 http://www.medworm.com/index.php?rid=1240931&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277460%26dopt%3DAbstract Authors: Baltaci AK, Mogulkoc R The aim of this study was to examine effects of pinealectomy and melatonin administration plasma leptin levels and its relationship with zinc in rats. The study was conducted on 40 adult male Sprague-Dawley rats. They were divided into four groups each containing 10 animals. Group 1 served as control. Group 2 was pinealectomized group. Animals in Group 3 were pinealectomized and injected with melatonin (3 mg/kg/day, ip). Group 4 received melatonin alone (3 mg/kg/day, ip). At the end of the experiments, all animals were decapitated and trunk blood collected. Plasma leptin and zinc levels were determined by radioimmunoassay and Atomic Absorption Spectrophotometer methods, respectively. Although mean weights of the animals at the beginning were not signific... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240931 Sat, 01 Dec 2007 05:00:00 +0100 1240931 http://www.medworm.com/index.php?rid=1240930&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277461%26dopt%3DAbstract Authors: Khan A, Haleem DJ To understand the role of serotonin (5-hydroxytryptamine; 5-HT)-1A receptors in the treatment of anxiety and the development of tolerance to benzodiazepines the present study was designed to monitor the responsiveness of postsynaptic 5-HT-1A receptors following repeated administration of diazepam and buspirone. Results show that tolerance in the anxiolytic profile is produced following repeated administration (2 weeks) of diazepam (2 mg/kg) but not buspirone (0.5 mg/kg). The behavioral effects of 8-OH-DPAT at a dose of 0.25 mg/kg were monitored 3 days after repeated administration of saline or buspirone or diazepam. The results show that 8-OH-DPAT elicited forepaw treading was smaller in repeated diazepam but not repeated buspirone injected rats, while hyperl... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240930 Sat, 01 Dec 2007 05:00:00 +0100 1240930 http://www.medworm.com/index.php?rid=1240929&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277462%26dopt%3DAbstract Authors: Alexandrova A, Petrov L, Kessiova M, Kirkova M The in vitro effects of alloxan and the product of its reduction dialuric acid (alone or in combination with copper ions) on lipid peroxidation, carbonyl content, GSH level and antioxidant enzyme activities in rat liver and kidney have been studied. The effects of Cu2+/alloxan and Cu2+/dialuric acid were compared with those of Fe3+/alloxan and Fe3+/dialuric acid. Unlike alloxan, dialuric acid increased liver and kidney lipid peroxidation; similar effects were registered in the presence of Fe3+. In the presence of Cu2+/dialuric acid, the lipid peroxidation was strongly inhibited and vice versa--the liver protein oxidation was increased. Alloxan and dialuric acid, as well as their combinations with Fe3+ had no effect on the total GS... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240929 Sat, 01 Dec 2007 05:00:00 +0100 1240929 http://www.medworm.com/index.php?rid=1240928&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277463%26dopt%3DAbstract Authors: Sounvoravong S, Nakashima MN, Wada M, Nakashima K We have previously reported that serotonin concentration was reduced in the brain of mice with neuropathic pain and that it may be related to reduction of morphine analgesic effects. To further prove this pharmacological action, we applied fluoxetine, a selective serotonin reuptake inhibitor, to determine whether it suppressed neuropathic pain and examined how its different administration routes would affect antinociceptive and antiallodynic effects of morphine in diabetic (DM) and sciatic nerve ligation (SL) mice, as models of neuropathic pain. Antiallodynia and antinociceptive effect of drugs were measured by using von Frey filament and tail pinch tests, respectively. Fluoxetine given alone, intracerebroventicularly (i.c.v., ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240928 Sat, 01 Dec 2007 05:00:00 +0100 1240928 http://www.medworm.com/index.php?rid=1240927&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277464%26dopt%3DAbstract Authors: Kovács P, Pállinger E, Csaba G Histone deacetylases can also influence acetylation of tubulin. In the present experiments, after 60 min of 10 microM trichostatin (TSA) treatment the structure and amount of tubulin and acetylated-tubulin were studied immunocytochemically, by using confocal microscopy and flow cytometry. In TSA-treated Tetrahymena cells deep fibres were never labeled with antibody to acetylated tubulin. Flow cytometry with anti acetylated-tubulin antibody demonstrated that in the contol cell populations there were weaker and stronger labelled parts. After TSA treatment in the weaker labeled part the cell number decreased, and in the stronger labeled part increased significantly: this means that after the histone deacetylase inhibitor TSA treatment the ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240927 Sat, 01 Dec 2007 05:00:00 +0100 1240927 http://www.medworm.com/index.php?rid=1240926&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277465%26dopt%3DAbstract Authors: Siviková K, Dianovský J, Piesová E, Holecková B The effects of low doses of cyclohexanol exposure were studied in mouse bone marrow cells including chromosome aberrations (CA), micronucleus (MN) and sister chromatid exchanges (SCE) as biomarkers. Capillaries with a tested agent that was evaporated continuously were placed in an experimental chamber for six weeks. No clastogenic and/or aneugenic effect of CA and MN induction was observed. A significant elevation of induced damage was achieved in the SCE study (p < 0.001) that has confirmed the early exposure of cyclohexanol to mice. PMID: 18277465 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240926 Sat, 01 Dec 2007 05:00:00 +0100 1240926 http://www.medworm.com/index.php?rid=1240925&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277466%26dopt%3DAbstract Authors: Siddique YH, Ara G, Beg T, Afzal M The anti-genotoxic effect of Ocimum sanctum L. extract was studied against the genotoxic effect induced by a synthetic progestin cyproterone acetate, on human lymphocytes using chromosomal aberrations, mitotic index, sister chromatid exchanges and replication index as a parameters. About 30 microM of cyproterone acetate was treated with O. sanctum L. infusion, at dosages of 1.075 x 10(-4), 2.125 x 10(-4) and 3.15 x 10(-4) g/ml of culture medium. A clear dose-dependent decrease in the genotoxic damage of cyproterone acetate was observed, suggesting a possible modulating role of the plant infusion. The results of the present study suggest that the plant infusion per se does not have genotoxic potential, but can modulate the genotoxicity of cypr... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240925 Sat, 01 Dec 2007 05:00:00 +0100 1240925 http://www.medworm.com/index.php?rid=1240924&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277467%26dopt%3DAbstract This study aimed to investigate the effect of vinpocetine on the acute hepatic injury caused in the rat by the administration of CCl4 in vivo. Vinpocetine (2.1, 4.2, 8.4 mg/kg) or silymarin (30 mg/kg) was given once daily orally simultaneously with CCl4 and for 15 days thereafter. Liver damage was assessed by determining serum enzyme activities and hepatic histopathology. Stained sections were subjected to morphometric evaluation using computerized image analyzer. The results showed that vinpocetine administered to CCl4-treated rats decreased the elevated alanine aminotransferase (ALT) by 49.3, 58.1 and 63.6%, aspartate aminotransferase (AST) by 10.5, 22.6 and 27.2% and alkaline phosphatase (ALP) by 52.5, 59.6 and 64.9%, respectively, and in a dose-dependent manner. Meanwhile, silymarin re... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240924 Sat, 01 Dec 2007 05:00:00 +0100 1240924 http://www.medworm.com/index.php?rid=1240923&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277468%26dopt%3DAbstract Authors: Ma GH, Huang XL Cytological and embryological studies on apomictic species Bothriochloa ischaemum L. were carried out. Our studies revealed that the chromosome number of its root apical cells was 40, indicating that it was a tetraploid cytotype. During the stage of microsporogenesis, meiosis seemed irregular, as the pairing chromosome number of microspore mother cell was more than 20. It was often found that some chromosomes did not assemble in the equatorial plane or moved to the two poles of the cell, a few laggards were seen. Multiporate pollens (22.3%) were often observed. The studies showed that a high frequency (87.8%) of 1-3 or more aposporous embryo sacs developed in one ovule of the species. The mature aposporous sac was usually characterized by an egg cell and one po... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240923 Sat, 01 Dec 2007 05:00:00 +0100 1240923 http://www.medworm.com/index.php?rid=1240922&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277469%26dopt%3DAbstract Authors: Pócsi I, Molnár Z, Pusztahelyi T, Varecza Z, Emri T The bulk formation of yeast-like (arthrospore-like) cells were typical in carbon-depleted submerged cultures of the high beta-lactam producer Penicillium chrysogenum NCAIM 00237 strain independently of the nitrogen-content of the culture medium. This morphogenetic switch was still quite common in carbon-starving cultures of the low-penicillin-producer strain P. chrysogenum ATCC 28089 (Wis 54-1255) when the nitrogen-content of the medium was low but was a very rare event in wild-type P. chrysogenum cultures. The mycelium-->yeast-like cell transition correlated well with a relatively high glutathione concentration and a reductive glutathione/glutathione disulfite (GSH/GSSG) redox balance in autolysing cultures, whi... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240922 Sat, 01 Dec 2007 05:00:00 +0100 1240922 http://www.medworm.com/index.php?rid=1240921&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18277470%26dopt%3DAbstract Authors: Seçkin S, Alsancak S, Başaran-Küçükgergin C, Uysal M Chronic administration of diazepam (DZP) caused an increase in malondialdehyde (MDA) levels and a decrease in glutathione (GSH) content. DZP also markedly lowered Ca2+ATPase activity. Treatment with Se plus vitamin E reduced MDA levels and increased GSH content. Our results suggest that, increased lipid peroxidation together with alteration in Ca2+ -ATPase activity may play a role in DZP induced hepatic injury and Se plus vitamin E treatment may contribute to the attenuation of DZP induced hepatotoxicity. PMID: 18277470 [PubMed - in process] (Source: Acta Biologica Hungarica) Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1240921 Sat, 01 Dec 2007 05:00:00 +0100 1240921 http://www.medworm.com/index.php?rid=913798&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899783%26dopt%3DAbstract Authors: Lukács A, Lengyel Z, Institóris L, Szabó A Young adult male Wistar rats were treated, by gavage, with 80 or 320 mg/kg Pb2+ (lead acetate), 0.4 or 1.6 mg/kg Hg2+ (mercuric chloride) or both by combining the lower doses. For combination with alcohol, ethanol was added to the rats' drinking water in 5 v/v %. After 12 weeks of treatment, electrophysiological recording was made from the somatosensory cortex in urethane anaesthesia. Evoked potentials obtained by stimulation of the whiskers were recorded. Both metals, and alcohol alone, increased significantly the latency of the evoked response. Alcohol seemed to abolish the effect of Pb, but not of Hg. Fatigue, calculated form the response amplitude, was increased by Pb and Hg treatment and this effect of Hg was reduc... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=913798 Sat, 01 Sep 2007 04:00:00 +0100 913798 http://www.medworm.com/index.php?rid=913797&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899784%26dopt%3DAbstract This study was designed to investigate the effects of excess (24.5 mg Se/kg feed) inorganic and organic dietary selenium supplementation on 3-week-old broilers. The experiments lasted 4 days. Intensity of lipid peroxidation processes (malondialdehyde, MDA) and the amount (reduced glutathione, GSH) and activity (glutathione peroxidase activity, GSHPx) of gluathione redox system were measured in blood plasma, red blood cell hemolysate and liver. Voluntary feed intake in the selenium-treated groups reduced remarkably. Elevated GSH concentration and GSHPx activity were measured in plasma and liver of both selenium-treated groups compared to the untreated control and the 'pair-fed' controls. The lipid peroxidation processes in the liver showed higher intensity than the control due to both selen... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=913797 Sat, 01 Sep 2007 04:00:00 +0100 913797 http://www.medworm.com/index.php?rid=913796&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899785%26dopt%3DAbstract Authors: Blázovics A, Sárdi E, Szentmihályi K, Váli L, Takács-Hájos M, Stefanovits-Bányai E Redox homeostasis can be considered as the cumulative action of all free radical reactions and antioxidant defences in different tissues, which provide suitable conditions for life. Transition metal ions are ubiquitous in biological systems. Beta vulgaris var. rubra (table beet root) contains several bioactive agents (e.g. betain, betanin, vulgaxanthine, polyphenols, folic acid) and different metal elements (e.g. Al, B, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, Zn), which act on the various physiological routes. Therefore we studied the effect of this metal rich vegetable on element content of the liver in healthy rats. Male Wistar rats (n = 7) (200 +/- 20 g) were treated... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=913796 Sat, 01 Sep 2007 04:00:00 +0100 913796 http://www.medworm.com/index.php?rid=913795&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899786%26dopt%3DAbstract Authors: Massányi P, Lukác N, Uhrín V, Toman R, Pivko J, Rafay J, Forgács Z, Somosy Z The aim of this study was to determine effects of Cd on the structure of ovary, oviduct and uterus after an experimental administration. Animals were divided into three groups. In group A rabbits received cadmium i.p. and were killed after 48 h. In group C Cd was administered p.o. for 5 month. The group K was the control. Decreased relative volume of growing follicles and increased stroma after Cd administration were detected. The number of atretic follicles was significantly higher after administration of Cd. The most frequent ultrastructural alterations observed were undulation of external nuclear membrane, dilatation of perinuclear cistern and endoplasmic reticulum. In all studi... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=913795 Sat, 01 Sep 2007 04:00:00 +0100 913795 http://www.medworm.com/index.php?rid=913794&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899787%26dopt%3DAbstract Authors: Móricz AM, Ott PG, Szilágyi M, Otta KH, Tyihák E The effect of Se(IV) and Cu(II) ions on the antibacterial activity of aflatoxins and ochratoxin A (mycotoxins) was studied in BioArena as a complex bioautographic system. In the presence of 0.23 and 0.46 mg/100 mL Se(IV) the inhibition zones of mycotoxins were decreased, however, lower concentration (0.046 mg/100 mL) increased the antibacterial effect of aflatoxin B1. Cu(II) (1.53 mg/100 mL) enhanced the toxicity of mycotoxins. The results supported the possible role of formaldehyde and its reaction products (e.g. 1O2, O3) in the antibacterial-toxic action of mycotoxins. Cu(II) can probably generate and mobilise the formaldehyde molecules and so it could increase the toxicity with its potential reaction products. ... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=913794 Sat, 01 Sep 2007 04:00:00 +0100 913794 http://www.medworm.com/index.php?rid=913793&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899788%26dopt%3DAbstract Authors: Anke M, Seifert M In 1930, Bortels showed that molybdenum is necessary for nitrogen fixation in Acetobacter, and in 1939 Arnon and Stout reported that molybdenum is essential for life in higher plants. Nitrogenase is the nitrogen-fixing enzyme complex, while nitrate reductase requires molybdenum for its activity. Molybdenum occurs in the earth crust with an abundance of 1.0-1.4 mg/kg. The molybdenum content of the vegetation is determined by the amount of this element in the soil and its pH-value. The weathering soils of granite, porphyry, gneiss and Rotliegendes produce a molybdenum-rich vegetation. Significantly poorer in Mo is the vegetation on loess, diluvial sands, alluvial riverside soils and especially on Keuper and Muschelkalk weathering soils, which produce legumes an... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=913793 Sat, 01 Sep 2007 04:00:00 +0100 913793 http://www.medworm.com/index.php?rid=913792&cid=s_32695_62_f&fid=32695&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899789%26dopt%3DAbstract Authors: Anke M, Seifert M, Holzinger S, Müller R, Schäfer U Molluscs and insects accumulate between 200 and 1050 microg Mo/kg of dry matter. Mice and vole species incorporate 350-650 microg Mo/kg, whereas shrews store 1500-2500 microg Mo/kg, i.e. insectivores have significantly higher molybdenum contents than rodents. The amounts of molybdenum accumulated by wild and domestic mammals are highest in the liver and kidneys, and lowest in muscle tissue and hair. The molybdenum status of mammals is reflected by all tissues tested except the heart. The best indicators of molybdenum deficiency and intoxication are liver, kidneys, blood and milk. The intrauterine storage of molybdenum in mammals is low. The milk delivers sufficient molybdenum amounts to the newborns. In man, the tra... Acta Biologica Hungarica journals http://www.medworm.com/rss/comments.php?id=913792 Sat, 01 Sep 2007 04:00:00 +0100 913792