MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Acta Histochemica' source. Mon, 06 Feb 2012 13:31:29 +0100 http://www.medworm.com/index.php?rid=5637029&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22277162%26dopt%3DAbstract Authors: Zaccone D, Sengar M, Lauriano ER, Pergolizzi S, Macri' F, Salpietro L, Favaloro A, Satora L, Dabrowski K, Zaccone G Abstract Swim bladders and lungs are homologous structures. Phylogenetically ancient actinopterygian fish such as Cladistians (Polypteriformes), Ginglymods (Lepisosteids) and lungfish have primitive lungs that have evolved in the Paleozoic freshwater earliest gnathostomes as an adaptation to hypoxic stress. Here we investigated the structure and the role of autonomic nerves in the physostome swim bladder of the cyprinid goldfish (Carassius auratus) and the respiratory bladder of lepisosteids: the longnose gar and the spotted gar (Lepisosteus osseus and L. oculatus) to demonstrate that these organs have different innervation patterns that are responsible for c... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5637029 Mon, 23 Jan 2012 05:00:00 +0100 5637029 http://www.medworm.com/index.php?rid=5637030&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22269467%26dopt%3DAbstract Authors: Loreto C, Lo Castro E, Musumeci G, Loreto F, Rapisarda G, Rezzani R, Castorina S, Leonardi R, Rusu MC Abstract Aquaporins (AQPs) are a family of hydrophobic membrane channel proteins. The expression of several AQP isoforms has been investigated in different human tissues, including the orofacial region. However, information on the role and localization of AQP1 in joints is limited, and no data are available on aquaporins in the normal temporomandibular joint (TMJ) disc. Sixteen human TMJ discs without degenerative changes were taken from fresh cadavers to investigate the presence and distribution of AQP1 by immunohistochemistry. The aim of the study was to gain additional insights into the biomolecular composition of aquaporins and their role in homeostasis of the TMJ. Por... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5637030 Sat, 21 Jan 2012 05:00:00 +0100 5637030 http://www.medworm.com/index.php?rid=5637031&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22265029%26dopt%3DAbstract Authors: Dall'aglio C, Polisca A, Boiti C, Ceccarelli P Abstract The aim of the present study was to investigate the presence and the distribution of leptin (Ob) and its receptor (ObR) in the feline placenta at term by means of immunohistochemical techniques. A few Ob-positive cells were observed scattered in the lamellae of the labyrinthine placenta. These cells had the morphological characteristics typical of the very abundant cells in the placenta of cats that can be considered as being decidual and, in some cases, syncytiotrophoblastic cells. A few ObR-positive cells were observed in the same placental portion and were mainly localized in the lamellae, showing morphological features typical of decidual and syncytiotrophoblastic cells. No other structure of the placenta or the u... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5637031 Thu, 19 Jan 2012 05:00:00 +0100 5637031 http://www.medworm.com/index.php?rid=5619214&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22261557%26dopt%3DAbstract Authors: Liquori GE, Mastrodonato M, Mentino D, Scillitani G, Desantis S, Portincasa P, Ferri D Abstract The characterization of mucus O-linked glycans in the proximal and distal mouse colon was performed by conventional histochemical methods and by lectin histochemistry in combination with enzymatic treatment (PNGase, α1,2 fucosidase, sialidase digestion), with and without prior desulfation. We demonstrated the presence of sialo- and sulfomucins in both the proximal and distal colon of the mouse. In the distal colon the sulfomucins were clearly prevalent, although there were always sialomucins with sialyl residues linked α2,6 to the subterminal galactose. Sialic acid was poorly O-acetylated, especially in the distal colon. The lectin binding pattern indicates a massive presence ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5619214 Tue, 17 Jan 2012 05:00:00 +0100 5619214 http://www.medworm.com/index.php?rid=5619215&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22257587%26dopt%3DAbstract Authors: Kasacka I, Janiuk I, Lewandowska A, Bekisz A, Lebkowski W Abstract Cocaine- and amphetamine-regulated transcript (CART) has been shown to play a critical role in appetite suppression, cell survival, thermoregulation, glucose sensing, stimulation of hormone secretion, as well as for the regulatory function of the islets of Langerhans. Although the principal site of CART synthesis has already been reported, our knowledge of the subject is mainly based on and limited to research conducted on animals owing to difficulties in obtaining human samples. Therefore, the primary goal of the reported study was an attempt to identify and localize CART in healthy human pancreas. Nineteen deceased subjects (donors of organs) with normal pancreas and alimentary tract were used in the stud... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5619215 Mon, 16 Jan 2012 05:00:00 +0100 5619215 http://www.medworm.com/index.php?rid=5619217&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22244448%26dopt%3DAbstract Authors: Cuajungco MP, Podevin W, Valluri VK, Bui Q, Nguyen VH, Taylor K Abstract Transmembrane (TMEM)-176A and 176B proteins belong to the MS4A family of proteins whose function in the immune system remains unclear. TMEM176A transcripts were previously shown to be elevated in liver cancer or kidney tissue with proteinuria, while marked changes in TMEM176B transcripts have been found in tolerated tissue allografts and neoplastic fibroblasts. To study the functional relationship between human TMEM176A and 176B and their putative link to cancer, we used polymerase chain reaction and biochemical assays. Here, we show that TMEM176A and 176B are widely expressed in all human tissues examined. Co-immunoprecipitation of heterologously expressed TMEM176A and 176B revealed direct physical i... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5619217 Wed, 11 Jan 2012 05:00:00 +0100 5619217 http://www.medworm.com/index.php?rid=5619216&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22244449%26dopt%3DAbstract Authors: Zuccari DA, Leonel C, Castro R, Gelaleti GB, Jardim BV, Moscheta MG, Regiani VR, Ferreira LC, Lopes JR, Neto DD, Esteves JL Abstract The use of prognostic markers for breast cancer is important for routine diagnosis and research. Interleukin-8 is a chemotactic cytokine produced by several cell types in response to inflammation, however, its expression, regulation and function are poorly understood. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. The present study investigated the correlation between interleukin-8 expression and breast cancer prognosis. Interleukin-8 expression was assessed in 72 women with mammary neoplasia by immunohistochemistry and the results were statistically correlated with clinical-pathological findings... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5619216 Wed, 11 Jan 2012 05:00:00 +0100 5619216 http://www.medworm.com/index.php?rid=5595564&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240017%26dopt%3DAbstract Authors: Park C, Choi S, Joo HG, Ahn M, Taniguchi K, Shin T Abstract The immunohistochemical localization of galectin-3, a β-galactoside-binding protein, was studied in the vomeronasal organ (VNO) of fetal, 1-day-old, and 6-month-old pigs. In all age groups, the porcine VNO consisted of vomeronasal sensory epithelium (VSE) located medially and non-sensory vomeronasal respiratory epithelium (VRE) located laterally. In the pig, the VNO epithelium increased in height with postnatal development from fetus to adult. In the VSE of all stages examined, galectin-3 immunostaining was seen in the supporting cells and free border, but not in receptor or basal cells. Galectin-3 immunostaining was seen in all layers of the VRE, and the intensity increased with postnatal development. In the lam... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5595564 Tue, 10 Jan 2012 05:00:00 +0100 5595564 http://www.medworm.com/index.php?rid=5558009&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209395%26dopt%3DAbstract Authors: Leonardi R, Loreto C, Talic N, Caltabiano R, Musumeci G Abstract Lubricin is a protein which contributes to the boundary lubrication, facilitating low friction levels at the interfacing surfaces of joints. In tendons and ligaments it facilitates the relative movement of collagen bundles. Its expression is affected by mechanical signals and cytokines. During application of orthodontic forces to teeth, there is a transduction of mechanical forces to the cells of the periodontal ligament (PDL), which triggers several biological reactions causing the synthesis of prostaglandins, cytokines and growth factors. The aim of the present study was to examine the immunolocalization of lubricin and to evaluate if it is time-dependently and differentially detected within the PDL followi... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5558009 Fri, 30 Dec 2011 05:00:00 +0100 5558009 http://www.medworm.com/index.php?rid=5558008&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209469%26dopt%3DAbstract Authors: El Hiba O, Gamrani H, Ahboucha S Abstract Hepatic encephalopathy is a common neuropsychiatric complication of acute and chronic liver failure. Whether brain structures with strategic positions in the interface of blood-brain barriers such as the circumventricular organs are involved in hepatic encephalopathy is not yet established. Among the circumventricular organs, the subcommissural organ secretes a glycoprotein known as Reissner's fiber, which condenses and forms an ever-growing thread-like structure into the cerebrospinal fluid. In the present work we describe the Reissner's fiber material within the subcommissural organ and its serotoninergic innervation in an animal model of chronic hepatic encephalopathy following bile duct ligation in experimental rats. The study ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5558008 Wed, 28 Dec 2011 05:00:00 +0100 5558008 http://www.medworm.com/index.php?rid=5558010&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22204823%26dopt%3DAbstract Authors: Alkafafy M, Sinowatz F Abstract Prenatal development of the epididymis was studied in bovine fetuses ranging from 10 to 90cm crown-rump length (CRL) (75-285 pcd). The studies aimed to apply both glycohistochemistry and immunohistochemistry for the detection of the differentiation of the developing prenatal epididymis. Both conventional histological and histochemical techniques were applied on paraffin sections of the epididymis from different fetal stages. Establishment of the urogenital junction between the extra-testicular rete testis and the mesonephric duct, via the growing efferent ductules (ductuli efferentes) was first evident in fetuses with 10cm CRL. At the fetal age of 110 pcd (24cm CRL), the mesonephric duct began to lengthen and coil forming three distinct regi... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5558010 Mon, 26 Dec 2011 05:00:00 +0100 5558010 http://www.medworm.com/index.php?rid=5544999&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22197484%26dopt%3DAbstract Authors: Alvarenga EM, Mondin M, Rodrigues VL, Andrade LM, Vidal BD, Mello ML Abstract The Malpighian tubule cell nuclei of male Panstrongylus megistus, a vector of Chagas disease, contain one chromocenter, which is composed solely of the Y chromosome. Considering that different chromosomes contribute to the composition of chromocenters in different triatomini species, the aim of this study was to determine the contribution of AT-, GC-, and methylated cytidine-rich DNA in the chromocenter as well as in euchromatin of Malpighian tubule cell nuclei of P. megistus in comparison with published data for Triatoma infestans. Staining with 4',6-diamidino-2-phenylindole/actinomycin D and chromomycin A(3)/distamycin, immunodetection of 5-methylcytidine and AgNOR test were used. The results r... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5544999 Thu, 22 Dec 2011 05:00:00 +0100 5544999 http://www.medworm.com/index.php?rid=5526970&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22178117%26dopt%3DAbstract Authors: Ngernsoungnern P, Ngernsoungnern A, Chaiseha Y, Sretarugsa P Abstract Animal eggs possess investments through which sperm must penetrate. The aim of the present study was to investigate the role of the egg coating, the vitelline envelope, during sperm-egg interactions in the black tiger shrimp, Penaeus monodon. The site(s) of primary binding between sperm and egg and the possible binding molecule(s) for sperm were identified. In vitro adsorption of the vitelline envelope protein onto the sperm surface showed that primary binding occurred between the sperm anterior spike of acrosome intact sperm and the vitelline envelope. Results from streptavidin blotting revealed that the component of the vitelline envelope that interacts with the sperm integral membrane protein is a 370... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5526970 Thu, 15 Dec 2011 05:00:00 +0100 5526970 http://www.medworm.com/index.php?rid=5526968&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22178118%26dopt%3DAbstract Authors: Du J, Fan Z, Ma X, Wu Y, Liu S, Gao Y, Wang J, Shen Y, Fan M, Wang S Abstract The Sonic hedgehog (SHH) signaling pathway plays many key roles in the development of Drosophila and vertebrate embryos, including regulating craniofacial development. The GLI family of transcription factors (GLI1, 2, and 3) mediates the SHH morphogenetic signal by regulating the expression of downstream target genes. Signaling aberrations seriously affect vertebrate development. To better understand the regulation of GLI transcription factors, we investigated the expression patterns of Gli1-3 during murine embryonic craniofacial development using in situ hybridization with whole-mounts and sections and quantitative real-time PCR. We found that Gli1 expression was mostly detected in the mesenchym... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5526968 Thu, 15 Dec 2011 05:00:00 +0100 5526968 http://www.medworm.com/index.php?rid=5526972&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22177215%26dopt%3DAbstract Authors: Srivastava N, Kumari U, Rai AK, Mittal S, Mittal AK Abstract Glycoproteins were analyzed by a range of histochemical methods in the epithelium of gills of Cirrhinus mrigala, a valuable food fish of great economic importance cultured extensively in India. The gills consist of gill arches, gill rakers, gill filaments and secondary lamellae. Major components of the epithelium of gill arches and gill rakers are epithelial cells, mucous goblet cells, rodlet cells, lymphocytes, eosinophilic granular cells and taste buds. In contrast, in the gill filament epithelium, rodlet cells and taste buds, and in secondary lamellae epithelium, rodlet cells, lymphocytes, eosinophilic granular cells and taste buds are not discernible. The epithelial cells, the mucous goblet cells and the eosi... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5526972 Wed, 14 Dec 2011 05:00:00 +0100 5526972 http://www.medworm.com/index.php?rid=5526971&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22177216%26dopt%3DAbstract Authors: Ishizeki K Abstract Our previous studies demonstrated that Meckel's chondrocytes, which are derived from ectomesenchyme, have the potential to transform into osteogenic phenotypes. The present study aimed to clarify the role of cell origin in the phenotypic transformation of chondrocytes. Cell pellets from ectomesenchyme-derived Meckel's cartilage and mesoderm-derived costal cartilage from green fluorescent protein (GFP)-transgenic mice were transplanted into the spleen for up to 4 weeks. Chondrocyte pellets from both cartilages adapted well to the splenic tissues and formed an alizarin red-positive calcified matrix, with increasing duration of transplantation. Following the production of cartilage-specific type II and type X collagens, newly-formed type I collagen appeare... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5526971 Wed, 14 Dec 2011 05:00:00 +0100 5526971 http://www.medworm.com/index.php?rid=5526976&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22172707%26dopt%3DAbstract Authors: Uluer ET, Aydemir I, Inan S, Ozbilgin K, Vatansever HS Abstract Aberrant activation of the JAK/STAT pathway may predispose to malignancy as a consequence of the deregulation of cell proliferation, differentiation or apoptosis such as in cancer of the blood, head and neck, and breast. In our study we aimed to investigate the effects of 5-fluorouracil (5-FU) and gemcitabine on a breast cancer cell line (MCF-7 cells) via the JAK/STAT pathway. Distribution of JAK1, JAK2, JAK3 and STAT2, STAT3, STAT4, STAT5 were evaluated on MCF-7 cells following gemcitabine and 5-FU treatment and in the absence of drug treatment by an indirect immunohistochemical method. It was observed that JAK1, JAK3, STAT5 and particularly STAT2 activation were more effective than the other JAK/STATs in bre... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5526976 Tue, 13 Dec 2011 05:00:00 +0100 5526976 http://www.medworm.com/index.php?rid=5526975&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22172708%26dopt%3DAbstract Authors: Kim JN, Chang IY, Kim JH, Kim JW, Park KS, Kim HI, Yoon SP Abstract Apurinic/apyrimidinic endonuclease (APE) acts as a regulator of p53 or vice versa in the cellular response to oxidative stress. Since oxidative stress-induced apoptosis is suggested in the pathophysiology of diabetic nephropathy, we proposed that APE may have a feasible role in the progression of diabetic complications. We investigated the interrelationship between APE and p53 in streptozotocin-induced diabetic rat kidneys. Variable parameters on kidneys were checked 12 weeks after streptozotocin administration with or without chitosan oligosaccharide (COS) treatment. Streptozotocin administration caused changes as seen in early diabetic nephropathy with increased kidney size, increased p53, decreased APE,... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5526975 Tue, 13 Dec 2011 05:00:00 +0100 5526975 http://www.medworm.com/index.php?rid=5526974&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22172709%26dopt%3DAbstract Authors: Chen XS, Chen XH, Ye JN, Cai QY, Zhan XL, Liu Z, Yao ZX Abstract The mechanisms underlying oligodendrocyte differentiation and myelination are still unclear, but understanding them will be critical for the development of therapies for multiple sclerosis. Inhibitor of DNA binding 2 (Id2) is a transcription factor thought to inhibit oligodendrocyte differentiation, however, it is not known whether the developmental changes and subcellular localization of Id2 are related to myelination. Therefore, we investigated the developmental changes in and the subcellular localization of Id2 immunoreactivity in the rat Corpus callosum, at post-natal developmental stages P0, P7, P14, P21, P42 and P90, by immunohistochemistry. Id2 expression increased from P0 to a peak at P42, the late st... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5526974 Tue, 13 Dec 2011 05:00:00 +0100 5526974 http://www.medworm.com/index.php?rid=5526973&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22172710%26dopt%3DAbstract Authors: Elgot A, El Hiba O, Gamrani H Abstract Dehydration is a powerful stimulus causing disequilibrium in homeostasis of water and electrolytes resulting from depletion in total body water. Most studies have focused on domestic and laboratory animals; however, the study of desert animals allows improved understanding about water balance and resistance to dehydration and associated behavioral changes, including those related to mood disorders. Meriones shawi (Shaw's Jird) is a desert rodent characterized by its resistance to long periods of thirst that can extend for several months. In the present study, M. shawi were subjected to water deprivation for 1 and 3months. We used 5-HT immunohistochemistry to evaluate the effects of prolonged dehydration on the serotoninergic system in... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5526973 Tue, 13 Dec 2011 05:00:00 +0100 5526973 http://www.medworm.com/index.php?rid=5507096&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22169810%26dopt%3DAbstract "Beyond the limit of histochemistry". The 14th International Congress of Histochemistry and Cytochemistry, Kyoto, Japan, August 26-29, 2012. Acta Histochem. 2011 Dec 9; Authors: Coleman R PMID: 22169810 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5507096 Fri, 09 Dec 2011 05:00:00 +0100 5507096 http://www.medworm.com/index.php?rid=5507097&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22154301%26dopt%3DAbstract Authors: Schumacher U, Nehmann N, Adam E, Mukthar D, Slotki IN, Horny HP, Flens MJ, Schlegelberger B, Steinemann D Abstract The multidrug-resistance 1 (MDR-1) P-glycoprotein (Pgp) is a transmembrane transporter system, which actively pumps cytotoxic drugs out of the cell. MDR-1 acquired in vitro differs from MDR-1 acquired in vivo, but has important consequences on the cellular phenotype and metastatic behavior. Here we report that the human colonic cancer cell line HT29 (MDR-1 negative) is more malignant than its MDR-1 overexpressing variant (HT29 MDR-1 positive). HT29 MDR-1 negative cells produce undifferentiated signet ring carcinomas when implanted subcutaneously into SCID mice, while HT29 MDR-1 positive cells form tumors with tubular structures, but without signet ring cells. ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5507097 Wed, 07 Dec 2011 05:00:00 +0100 5507097 http://www.medworm.com/index.php?rid=5507098&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22137777%26dopt%3DAbstract In conclusion, elevated expression of PEG10 is likely to be involved in the pathophysiology of preeclampsia. Further studies are needed to elucidate the precise role of PEG10 in preeclampsia. PMID: 22137777 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5507098 Thu, 01 Dec 2011 05:00:00 +0100 5507098 http://www.medworm.com/index.php?rid=5472668&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22133296%26dopt%3DAbstract Authors: Miladi-Abdennadher I, Abdelmaksoud-Dammak R, Ayed-Guerfali DB, Ayadi L, Khabir A, Amouri A, Frikha F, Tahri N, Ellouz S, Frikha M, Sellami-Boudawara T, Mokdad-Gargouri R Abstract Cyclo-oxygenase 2 (COX-2) and E-cadherin are promising biomarkers for cancer diagnosis and therapy. The aim of this study was to examine the expression of these two proteins in primary colorectal adenocarcinomas and to investigate their association with clinicopathological characteristics including survival of patients. Immunostaining of E-cadherin and COX-2 was assessed in 70 primary colorectal adenocarcinomas from Tunisian patients. Membranous E-cadherin immunostaining and cytoplasmic COX-2 expression were observed in 74.3% and 68.6% of cases respectively. A significant association was found bet... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5472668 Tue, 29 Nov 2011 05:00:00 +0100 5472668 http://www.medworm.com/index.php?rid=5472669&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22130252%26dopt%3DAbstract Authors: Jiang L, Lv X, Li J, Li J, Li X, Li W, Li Y Abstract Dysregulation of microRNA-21 plays critical roles in tumor initiation and progression. The purpose of this study was to investigate the status of microRNA-21 expression in human cutaneous malignant melanoma and determine its clinical significance. TaqMan(®) real-time RT-PCR assay was performed to examine the expression of microRNA-21 in 10 cases of dysplastic nevi, 86 cases of primary cutaneous melanomas, 10 cases of melanoma metastases. The correlation of microRNA-21 expression with clinicopathological factors or prognosis of patients with cutaneous melanoma was statistically analyzed. Additionally, the effects of microRNA-21 expression on growth, apoptosis and chemo- or radiosensitivity of melanoma cells were also inv... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5472669 Mon, 28 Nov 2011 05:00:00 +0100 5472669 http://www.medworm.com/index.php?rid=5472670&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119409%26dopt%3DAbstract In conclusion, our results show that GPC3 is specifically overexpressed in HCC tissue and may be regarded as a potential marker for differential diagnostic hepatocellular carcinoma. PMID: 22119409 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5472670 Thu, 24 Nov 2011 05:00:00 +0100 5472670 http://www.medworm.com/index.php?rid=5472671&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119208%26dopt%3DAbstract This study aimed to examine the influence of pregnancy on responsiveness of the pituitary-adrenal (PA) axis to dexamethasone (Dx) in rats. Dams and virgins were injected with Dx in three consecutive daily doses of 1.0, 0.5, 0.5mg/kg/bw, starting from day 16 of pregnancy, and sacrificed 24 and 72h after termination of treatment. Immunocytochemical, morphometrical and biochemical methods were used to assess pituitary and adrenal function. Three-way ANOVA revealed Dx inhibition of the PA axis in virgin and pregnant females after 24 and 72h. Pregnant females demonstrated a significantly lower (p<0.05) degree of suppression regarding ACTH volume density and blood concentrations and the volume and number of the zona fasciculata and zona reticularis cells, comparing with virgin females 72h fol... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5472671 Wed, 23 Nov 2011 05:00:00 +0100 5472671 http://www.medworm.com/index.php?rid=5472673&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22113176%26dopt%3DAbstract This study indicates that smoking may play a significant role in the development of endocrine disturbances in the development of chronic pancreatitis. PMID: 22113176 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5472673 Mon, 21 Nov 2011 05:00:00 +0100 5472673 http://www.medworm.com/index.php?rid=5472672&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22113177%26dopt%3DAbstract Authors: Petricevic J, Punda H, Brakus SM, Vukojevic K, Govorko DK, Alfirevic D, Kvesic A, Saraga-Babic M Abstract The spatial and temporal distribution of epithelial membrane antigen (EMA), mesothelin and nestin was immunohistochemically analyzed in developing and adult human serous membranes and mesotheliomas in order to detect possible differences in the course of mesenchymal to epithelial transformation, which is associated with differentiation of mesothelial cells during normal development and tumorigenesis. Pleura and pericardium developing from the visceral mesoderm gradually transform into mesothelial cells and connective tissue. EMA appeared in mesothelium of both serous membranes during the early fetal period, whereas during further development, EMA expression was retaine... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5472672 Mon, 21 Nov 2011 05:00:00 +0100 5472672 http://www.medworm.com/index.php?rid=5472674&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22104449%26dopt%3DAbstract Authors: Kottorou AE, Antonacopoulou AG, Dimitrakopoulos FI, Tsamandas AC, Scopa CD, Petsas T, Kalofonos HP Abstract NFY-C, a subunit of the transcription factor NFY, binds to the promoters of several eukaryotic genes, including cell cycle-related genes. RORA is a steroid hormone receptor implicated in a range of important cellular processes. We evaluated the expression of NFY-C and RORA in colorectal adenocarcinomas and normal colonic tissue. NFY-C expression was elevated in adenocarcinomas. Moreover, NFY-C mRNA levels correlated with time to disease progression, while NFY-C protein expression was significantly higher in metastatic disease. RORA expression was downregulated in CRC adenocarcinomas compared to normal controls and correlated with time to disease progression. The role... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5472674 Fri, 18 Nov 2011 05:00:00 +0100 5472674 http://www.medworm.com/index.php?rid=5414277&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22078489%26dopt%3DAbstract Authors: Roux N, Léveque S, Freguin-Bouilland C, Dautreaux B, Proust F, Thuillez C, Plissonnier D Abstract Vascular rejection is characterized by intimal proliferation and perivascular inflammation. We hypothesize that recipient stem cell therapy could prevent or ameliorate the development of the obliterative lesion. We studied the kinetic expression of three cytokines (SDF-1, MCP-1, VEGF) implicated in mobilization, homing and differentiation of progenitor cells during vascular aggression. An aortic allograft mouse model was used (BALBc donor-C57BL6/j recipient). Ten mice were sacrificed at Day 0, D1, D3, D6, D9, D12, and D20. Cytokine rates were measured in blood and in graft tissue by an ELISA technique. Results showed that in the allograft, SDF-1 and VEGF tissue levels were si... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5414277 Wed, 09 Nov 2011 05:00:00 +0100 5414277 http://www.medworm.com/index.php?rid=5414278&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22075213%26dopt%3DAbstract In conclusion, UV radiation of shorter wavelength (UVB) induced an increase in expression of all MMPs except MMP-14. It is suggested that overexpression of MMPs in the corneal epithelium contributes to the damaging effect of UVB radiation to the cornea. PMID: 22075213 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5414278 Tue, 08 Nov 2011 05:00:00 +0100 5414278 http://www.medworm.com/index.php?rid=5377327&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22036174%26dopt%3DAbstract Authors: Pirone A, Ding BA, Giannessi E, Coli A, Stornelli MR, di Cossato MM, Piano I, Lenzi C Abstract The distribution of Glucagon-like peptide 1 (GLP-1) was investigated in the gastrointestinal tract of the pheasant using immunohistochemistry. GLP-1 immunoreactive cells were common in the small intestine, in the proventriculus and in the pancreas. Immunostained cells were not seen in the crop, in the gizzard and in the large intestine. Double labelling demonstrated that GLP-1 and pituitary adenylate cyclase-activating polypeptide (PACAP) were occasionally co-localized only in the duodenal villi. In contrast to what was previously described in the chicken and ostrich, we noted GLP-1 positive cells in the duodenum. These data were consistent with the presence of proglucagon mRNA i... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5377327 Fri, 28 Oct 2011 04:00:00 +0100 5377327 http://www.medworm.com/index.php?rid=5377328&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22018781%26dopt%3DAbstract Authors: Wei D PMID: 22018781 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5377328 Fri, 21 Oct 2011 04:00:00 +0100 5377328 http://www.medworm.com/index.php?rid=5377332&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22000861%26dopt%3DAbstract Authors: Jardim BV, Moschetta MG, Gelaleti GB, Leonel C, Regiani VR, de Santi Neto D, Bordin-Junior NA, Perea SA, Zuccari DA Abstract Breast cancer is the most frequent cancer in women worldwide. Prognostic markers are important for diagnosis, allowing therapeutic strategies to be defined more efficiently. The expression of the glutathione S-transferase pi isoenzyme (GSTpi) in tumor cells has been evaluated as a predictor of prognosis and in response to cytotoxic treatments. Its immunoexpression was assessed in 63 women diagnosed with invasive ductal carcinoma in a retrospective study. The results were statistically correlated with clinicopathological parameters of patients. The results showed that high GSTpi expression was related to p53-positive tumors, grade III histology, large... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5377332 Thu, 13 Oct 2011 04:00:00 +0100 5377332 http://www.medworm.com/index.php?rid=5377331&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22000862%26dopt%3DAbstract Authors: Lukáčová N, Kisucká A, Pavel J, Hricová L, Kucharíková A, Gálik J, Maršala M, Langfort J, Chalimoniuk M Abstract Using immunohistochemistry, we detected the expression of neuronal nitric oxide synthase (nNOS) in ventral medullary gigantocellular reticular nuclei and in the lumbosacral spinal cord 10 days after thoracic transection in experimental rabbits. We tried to determine whether neurons located below the site of injury are protected by the calcium binding protein parvalbumin (PV). Changes of nNOS immunoreactivity (IR) in spinal cord were correlated with the level of nNOS protein in dorsal and ventral horns. Ten days after transection, nNOS was upregulated predominantly in lateral gigantocellular nuclei. In the spinal cord, we revealed a significant increase ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5377331 Thu, 13 Oct 2011 04:00:00 +0100 5377331 http://www.medworm.com/index.php?rid=5377330&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22000863%26dopt%3DAbstract The objective of our study was to examine in experimental rats how maternal prenatal stress induced by a forced swim test affects offspring afferent spinal responses mediated by stimulation of vaginocervical receptors. The activation of spinal cord neurons showing c-fos expression was examined following vaginocervical mechanical stimulation in adult rats, which were the offspring of dams exposed to gestational stress from E10 until delivery. Vaginocervical stimulation of both prenatal-stressed and non-prenatal-stressed rats induced an increase in immunoreactive protein in the spinal cord ranging from T12 to S1 segmental levels. However, a significantly higher (40%) increase in the expression of Fos-immunoreactive neurons was observed in vaginocervical stimulated prenatally stressed rats th... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5377330 Thu, 13 Oct 2011 04:00:00 +0100 5377330 http://www.medworm.com/index.php?rid=5377329&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22000864%26dopt%3DAbstract Authors: Cheng MW, Wang BC, Weng ZQ, Zhu XW Abstract Polo-like kinase 1 (PLK1), a variety of serine/threonine-protein kinase, has been reported to play important roles in malignant transformation. The purpose of this study was to investigate the clinicopathological significance of PLK1 expression in malignant glioma. A semi-quantitative RT-PCR assay was performed to detect the expression of PLK1 mRNA in 68 cases of glioma tissues and corresponding non-cancerous brain tissues. Additionally, the correlation of PLK1 mRNA expression with clinicopathological factors or prognosis of glioma patients was statistically analyzed. Multivariate analysis of prognostic factors was performed using the Cox proportional hazard model. Small interfering RNA was used to knockdown PLK1 expression in a ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5377329 Thu, 13 Oct 2011 04:00:00 +0100 5377329 http://www.medworm.com/index.php?rid=5294303&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21975054%26dopt%3DAbstract Authors: Choi S, Park C, Ahn M, Lee JH, Shin T Abstract Arginase 1 and arginase 2 catalyze the hydrolysis of arginine to ornithine and urea. The localization of these enzymes was studied in various tissues in Sprague-Dawley rats by immunohistochemistry and Western blotting. Western blot analysis showed that both arginase 1 and 2 were differentially expressed in the various organs examined. Arginase 1 was expressed at high levels in the liver, at moderate levels in the pancreas, and at low levels in the cerebrum, cerebellum, spinal cord, stomach, small and large intestines, kidneys, lungs, and spleen. The levels of arginase 2 immunoreactivity were high in the kidneys and pancreas, and moderate in the cerebrum, spinal cord, stomach, small intestine, large intestine, and lungs; the le... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5294303 Mon, 03 Oct 2011 04:00:00 +0100 5294303 http://www.medworm.com/index.php?rid=5294302&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21975055%26dopt%3DAbstract Authors: Petrušić V, Zivković I, Stojanović M, Stojićević I, Marinković E, Dimitrijević L Abstract Tetanus neurotoxin (TeNT) represents a potent toxin that binds to its receptors on neurons and inhibits the release of neurotransmitters. Additionally, its fragments are used to transport pharmacological substances to neuronal cell bodies. The main objective of this study was the development of a suitable model system to study internalization of the TeNT. We have produced a monoclonal antibody (MoAb) specific for TeNT by hybridoma technology, after immunization of BALB/c mice with tetanus toxoid, and have named it T-62. The immunochemical characteristics of MoAb T-62 were tested using ELISA, PAGE and immunoblotting. Finally, we have used an immunohistochemical method to detect... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5294302 Mon, 03 Oct 2011 04:00:00 +0100 5294302 http://www.medworm.com/index.php?rid=5294304&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21962635%26dopt%3DAbstract Authors: Piras M, Tandler B, Tomassini Barbarossa I, Piludu M Abstract Serous granules in the human parotid gland have a well-defined substructure, consisting of a dense spherule suspended in a moderately dense matrix. Immunogold labeling with an antibody against carbonic anhydrase VI revealed that this enzyme is localized within the matrix and is absent from the spherule. This location matches that of a number of other salivary gland proteins. Cell organelles involved in the secretory pathway are devoid of label. Labeling was not observed in any ductular component of the gland. PMID: 21962635 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5294304 Wed, 28 Sep 2011 04:00:00 +0100 5294304 http://www.medworm.com/index.php?rid=5273609&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21955422%26dopt%3DAbstract Authors: Leonardi R, Rusu MC, Loreto F, Loreto C, Musumeci G Abstract Lubricin, which is a boundary joint lubricant, was investigated immunohistochemically in the bilaminar zone (BZ) of the human temporomandibular joint (TMJ), without any degenerative changes. Immunohistochemistry for lubricin detection was carried out on 33 TMJ discs obtained from 17 cadavers. Sections were incubated with diluted rabbit polyclonal anti-lubricin antibody and scored according to the percentage of lubricin immunopositive cells. Three different TMJ disc tissue compartments were analyzed, namely: the upper lamina, the inferior lamina and the loose connective tissue in the space between the laminae. The Mann-Whitney U test was used to compare protein expression (lubricin) among disc specimens' regions. ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5273609 Mon, 26 Sep 2011 04:00:00 +0100 5273609 http://www.medworm.com/index.php?rid=5273610&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21937094%26dopt%3DAbstract Authors: Aller MA, Martinez V, Corcuera MT, Benito J, Traver E, Gómez-Aguado F, Vergara P, Arias J Abstract Mast cells are involved in various liver diseases and appear to play a broader pathogenic role than originally thought. They may participate in the splanchnic alterations related to a porto-systemic shunt. To verify this hypothesis we studied the serum and hepatic histological changes in rats four weeks after an end-to-side portacaval shunt. In this experimental model of chronic liver insufficiency we also assessed the mucosal mast cells (MMC) and connective tissue mast cells (CTMC) in the liver, mesenteric lymph nodes and small intestine, as well as the serum levels of rat mast cell protease-II (RMCP-II). The results show liver and testes atrophy, with hypoalbuminemia (p=0.... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5273610 Mon, 19 Sep 2011 04:00:00 +0100 5273610 http://www.medworm.com/index.php?rid=5241402&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21925717%26dopt%3DAbstract Authors: Wang Q, Gao Y, Tang Y, Ma L, Zhao M, Wang X Abstract Ras interaction/interference 1 (RIN1), originally identified as a Ras effector protein, has been implicated in tumorigenesis and development of human cancers. The aim of this study was to detect RIN1 expression in human non-small cell lung cancer (NSCLC) and to analyze its association with prognosis of NSCLC patients. Quantitative real-time RT-PCR was performed to examine the expression of RIN1 mRNA in 25 cases of NSCLC and corresponding non-tumor tissue samples. Immunohistochemistry was performed to detect the expression of RIN1 in 90 NSCLC tissues. We found that the expression levels of RIN1 mRNA in NSCLC tissues were significantly higher than those in corresponding non-tumor tissues. High-level RIN1 expression was obs... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5241402 Fri, 16 Sep 2011 04:00:00 +0100 5241402 http://www.medworm.com/index.php?rid=5241401&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21925718%26dopt%3DAbstract Authors: Habata I, Yasui T, Fujimori O, Meyer W, Tsukise A Abstract Saliva is known to protect the oral cavity and contains glycoproteins and antimicrobial substances. The distribution of these salivary secretions was studied in the labial glands of the Japanese miniature (Shiba) goat using lectin histochemical and immunohistochemical methods. The mucous acinar cells of the labial glands exhibited glycoconjugates with different saccharide residues, such as GalNAcα1-3GalNAc, Galβ1-4GalNAc, β-d-GlcNAc and sialic acid linked to α2-6Gal/GalNAc. Furthermore, α-d-Man, α-l-Fuc, α-d-GalNAc, β-d-Gal and sialic acid residues were present, in particular, in the serous demilunar cells. Antimicrobial substances (lysozyme, IgA, lactoferrin and β-defensin) were shown to be mainly immunol... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5241401 Fri, 16 Sep 2011 04:00:00 +0100 5241401 http://www.medworm.com/index.php?rid=5225848&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21920588%26dopt%3DAbstract The objective of the research was to study the changes of the major organelles, endoplasmic reticulum (ER) and mitochondria, in mammary epithelial cells of the Chinese Holstein dairy cow during mammogenesis. For this purpose, a mammary epithelial cell bank was established from 9 selected Chinese Holstein dairy cows using collagenase I digestion and attachment culture biotechniques. This cell bank included 9 samples at stages of pregnancy, lactation and involution. The changes of ER and mitochondria in the mammary cells were observed at the subcellular level using living cell fluorescent labeling and laser confocal microscopy. Subsequently, the area of integrated optical density of each sample was calculated to determine changes of ER and mitochondria in the mammary epithelial cells. The re... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5225848 Tue, 13 Sep 2011 04:00:00 +0100 5225848 http://www.medworm.com/index.php?rid=5215841&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21906788%26dopt%3DAbstract In conclusion, both morphometric and glycohistochemical findings displayed regional species-specific and potentially functional relevant characteristics. PMID: 21906788 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5215841 Wed, 07 Sep 2011 04:00:00 +0100 5215841 http://www.medworm.com/index.php?rid=5215842&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21890179%26dopt%3DAbstract Authors: Zhang XJ, Wen XX, Zhao L, He JP Abstract The transforming growth factor beta (TGF-β) superfamily exerts a wide range of effects on biological events, including spermatogenesis. Smad proteins are downstream signal mediators, which transduce TGF-β signals from the cell surface to the nucleus. Smad4 protein is the common transducer of the TGF-β superfamily that participates in the signaling of all the members of TGF-β superfamily. Smad4 is expressed in the mammalian testis and is believed to play an important role during testicular development and spermatogenesis. Information about Smad4 distribution and function in the testis of birds, including the domestic fowl, is still unclear. In the current study, our objective was to clarify the signal transduction pathway of the ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5215842 Fri, 02 Sep 2011 04:00:00 +0100 5215842 http://www.medworm.com/index.php?rid=5139304&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20598360%26dopt%3DAbstract Authors: Knapczyk-Stwora K, Durlej M, Bilinska B, Slomczynska M Abstract The development of uterine glands is characterized by the proliferation of epithelial cells and by estrogen receptor alpha (ERα) expression in the nascent glandular epithelium. It is known that androgen receptors are present in the porcine uterus during prenatal development and the neonatal window, when adenogenesis occurs. Therefore, the objective of the study was to determine whether the effects of maternal or neonatal administration of the anti-androgen, flutamide, could entail changes in the presence of ERα and proliferation of uterine cells in neonatal and three-month-old pigs. Following prenatal flutamide exposure, morphological differences and the acceleration of uterus differentiation marked by ERα ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5139304 Fri, 19 Aug 2011 11:46:53 +0100 5139304 http://www.medworm.com/index.php?rid=5139300&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21839495%26dopt%3DAbstract In conclusion, our results suggest that IGFBP-4 may play a potential role in pancreatic injury and regeneration in a murine model of acute pancreatitis induced by l-arginine. PMID: 21839495 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5139300 Tue, 09 Aug 2011 23:00:00 +0100 5139300 http://www.medworm.com/index.php?rid=5139302&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21831412%26dopt%3DAbstract Authors: Kong W, Yang Y, Feng L, Zhang Y Abstract A Smad nuclear interacting protein 1 (SNIP1) homologous gene was identified in amphioxus. Phylogenic analysis showed that SNIP1 proteins from different species share a highly conserved FHA domain at the C-terminus, but their N-terminus varies remarkably. The genomic structure of SNIP1 varies in different species, especially at the 5' end. Through in situ hybridization, we studied SNIP1 expression patterns in amphioxus and zebrafish embryos. Amphioxus SNIP1 transcripts were specifically located in the notochord in larval and adult stages. In zebrafish, however, snip1 transcripts were specifically located not only in the notochord, but also in the rhombencephalic ventricle, otic vesicles and pectoral fin buds. This is the first report... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5139302 Sun, 07 Aug 2011 23:00:00 +0100 5139302 http://www.medworm.com/index.php?rid=5139301&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21831413%26dopt%3DAbstract Authors: Zaccone D, Dabrowski K, Lauriano ER, de Pasquale A, Macrì D, Satora L, Lanteri G Abstract Anatomical and functional studies on the autonomic innervation as well as the location of airway receptors in the air-bladder of lepisosteids are very fragmentary. These water-breathing fishes share in common with the bichirs the presence of a glottis (not a ductus pneumaticus) opening into the esophagus. In contrast to a high concentration of neuroepithelial cells (NECs) contained in the furrowed epithelium in the lung of Polypterus, these cells are scattered as solitary cells in the glottal epithelium, and grouped to form neuroepithelial bodies (NEBs) in the mucociliated epithelium investing the main trabeculae in the air-bladder of Lepisosteus osseus and L. oculatus. The present i... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5139301 Sun, 07 Aug 2011 23:00:00 +0100 5139301 http://www.medworm.com/index.php?rid=5139303&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21820160%26dopt%3DAbstract Authors: Roy VK, Krishna A Abstract The aim of this study was to compare the changes in the expression of hormone sensitive lipase (HSL) and organic cation transporter 2 (OCTN2) in different regions of the female reproductive tract of bats (Scotophilus heathii) during the period of sperm storage. Western blot analysis revealed that both HSL and OCTN2 showed elevated expression in the utero-tubal junction, the site of sperm storage, compared to the vagina, cervix and uterus. An immunohistochemical study showed localization of HSL and OCTN2 in the cytoplasm of luminal epithelial cells in the utero-tubal junction of S. heathii, which suggests a role of fatty acids metabolism at the site of sperm storage. Furthermore, this study showed the presence OCTN2 immunostaining in the tail of s... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5139303 Tue, 02 Aug 2011 23:00:00 +0100 5139303 http://www.medworm.com/index.php?rid=5090589&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21803403%26dopt%3DAbstract Authors: Turk N, Dağistanli FK, Sacan O, Yanardag R, Bolkent S The aim of the study was to evaluate the effect of ghrelin treatment on obestatin, insulin gene expression and biochemical parameters in the pancreas of newborn-streptozocin (STZ) diabetic rats. Rats were divided into 4 groups. Group I: control rats treated with physiological saline; group II: control rats treated with 100μg/kg/day ghrelin; group III: two days after birth rats that received 100mg/kg STZ injected as a single dose to induce neonatal diabetes; group IV: neonatal-STZ-diabetic rats treated with ghrelin for four weeks. Sections of the pancreas were examined with immunohistochemistry for the expression of obestatin and insulin and in situ hybridization for the expression of insulin mRNA. The blood glucose levels... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5090589 Thu, 28 Jul 2011 23:00:00 +0100 5090589 http://www.medworm.com/index.php?rid=5090593&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21802714%26dopt%3DAbstract Authors: Nara T, Yasui T, Meyer W, Tsukise A The distribution of sialic acids and antimicrobial products (lysozyme, β-defensin-1, lactoferrin, IgA) in the anal glands of miniature pig was studied by glycoconjugate histochemistry and immunohistochemistry. The glandular acini of these glands exhibited considerable amounts of sialoglycoconjugates that terminated in Siaα2-6Gal/GalNAc or Siaα2-3Gal1-4GlcNAc, including O-acetylated sialic acids. Additionally, all antimicrobial products examined could be demonstrated in the anal glands, especially in the serous cells. The results obtained are discussed with regard to the functional significance of the anal glands. Our observations corroborated the view that sialic acids closely interact with defense cells and antimicrobial substances in th... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5090593 Wed, 27 Jul 2011 23:00:00 +0100 5090593 http://www.medworm.com/index.php?rid=5090616&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21798576%26dopt%3DAbstract Authors: Mahmoud YI Hibiscus sabdariffa extract is a popular beverage in many tropical and sub-tropical countries. Although, Hibiscus tea is known for its medicinal effects for thousands of years, scientific evidence of its systemic safety is very limited. The current study aimed to assess the potential adverse effects of H. sabdariffa extract on sperm morphology and testicular ultrastructure of albino mice. Thirty adult male albino mice were divided into three equal groups and were given: (a) distilled water, (b) cold Hibiscus aqueous extract, and (c) boiled Hibiscus aqueous extract. Hibiscus extract was administered orally daily for 4 weeks in a dose of 200mg/kg body weight/mouse. Twenty-four hours after the last treatment, mice were decapitated and the testes and epididymides were e... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5090616 Mon, 25 Jul 2011 23:00:00 +0100 5090616 http://www.medworm.com/index.php?rid=5090617&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21794900%26dopt%3DAbstract Authors: Franchini A, Bertolotti E A morphofunctional analysis of the thymus from differently aged Xenopus laevis tadpoles during regeneration of the tail is reported. In stage 50 larvae, competent to regenerate, the appendage cut provoked thymic structural modifications that affected the medullary microenvironment cells and changes in TNF-α immunoreactivity. Mucocyte-like cells, multicellular epithelial cysts, myoid cells and cells immunoreactive to TNF-α increased in number. Increased numbers of lymphocytes were also found in regenerating areas and, at the end of regeneration, thymic structural and immunocytochemical patterns were restored to control levels. The observed cellular responses and the induction of molecules critical for thymus constitutive processes suggest a stimulati... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5090617 Sun, 24 Jul 2011 23:00:00 +0100 5090617 http://www.medworm.com/index.php?rid=5090618&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21782216%26dopt%3DAbstract This study investigated whether topical application of PerClot(®) could improve impaired wound healing in Sprague-Dawley (SD) rats. Full-thickness skin wounds were created on the back of the rats. Immediately, PerClot(®) was introduced into the wound bed, while wounds receiving starch or nothing served as controls. Wound closure was monitored using well-recognized wound-healing parameters: histological examination for inflammatory cells and fibroblast infiltration, newly formed capillaries, and collagen deposition. Meanwhile, transforming growth factor (TGF-β1) was measured by immunochemistry. Wound closure was significantly accelerated by local application of PerClot(®). Furthermore, PerClot(®)-treated wounds showed significantly increased fibroblast numbers at 5 days post-wounding, ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5090618 Tue, 19 Jul 2011 23:00:00 +0100 5090618 http://www.medworm.com/index.php?rid=5090619&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21774970%26dopt%3DAbstract Authors: Marini M, Bonaccini L, Thyrion GD, Vichi D, Parretti E, Sgambati E The aim of the study was to investigate the content and distribution of sugar residues in placentas from pregnancies complicated by hypertensive disorders. Placentas from women with uncomplicated pregnancies (group 1), pregnancies complicated by gestational hypertension (group 2), pregnancies complicated by pre-eclampsia (group 3), pregnancies complicated by pre-eclampsia with HELLP syndrome (hemolysis, elevated liver enzymes and low platelets) (group 4) were collected. Lectins: ConA, WGA, PNA, SBA, DBA, UEA I, GNA, DSA, MAA, SNA, in combination with chemical and enzymatic treatments, were used. Data showed a decrease and/or lack of α-d-mannose, α-d-glucose and d-galactose-(β1-4)-N-acetyl-d-glucosamine in pl... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5090619 Sun, 17 Jul 2011 23:00:00 +0100 5090619 http://www.medworm.com/index.php?rid=5040776&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21764105%26dopt%3DAbstract Authors: Takeuchi A, Fukazawa S, Chida K, Taguchi M, Shirataka M, Ikeda N Connexin 32s (Cx32s) were immunolocalized in fetal rat hepatocytes and their distribution was determined qualitatively. We used image analysis using a quantitative index (QI) of Cx32 (QI Cx32) defined as the area of Cx32s/number of cells in cultured fetal rat hepatocytes. Hepatocytes from livers of fetal rats were separated by collagenase digestion and low centrifugation on gestational day 17. Cells were cultured for 3 days in dexamethasone (DEX)-supplemented medium (Dex0). The medium was replaced with fresh medium and cells were continuously cultured for 3 days with DEX or epidermal growth factor supplemented medium (Dex3 or EGF3). After culture termination, cells were fixed and stained using the fluorescein-lab... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5040776 Thu, 14 Jul 2011 23:00:00 +0100 5040776 http://www.medworm.com/index.php?rid=5040775&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21764106%26dopt%3DAbstract Authors: Mosevitsky MI, Snigirevskaya ES, Komissarchik YY Immunoelectron microscopy was used to locate the proteins BASP1 and MARCKS in the post-meiotic spermatids of male rat testis. It was shown that in early spermatids, BASP1 and MARCKS accumulate in chromatoid bodies, which are characteristic organelles for these cells. During spermatogenesis, while the spermatid nucleus is still active, the chromatoid body periodically moves to the cell nucleus and absorbs the precursors of definite mRNAs and small RNAs. mRNAs are preserved in the chromatoid body until the corresponding proteins are needed, but their "fresh" mRNA cannot be formed due to the nucleus inactivation. The chromatoid body (0.5-1.5μm in diameter) has a cloud-like fibrous appearance with many fairly round cavities. In the... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5040775 Thu, 14 Jul 2011 23:00:00 +0100 5040775 http://www.medworm.com/index.php?rid=5040774&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21764425%26dopt%3DAbstract Authors: de la Fuente V, Rodríguez N, Amils R Ferritin is of interest at the structural and functional level not only as storage for iron, a critical element, but also as a means to prevent cell damage produced by oxidative stress. The main objective of this work was to confirm by immunocytochemistry the presence and the subcellular distribution of the ferritin detected by Mösbauer spectroscopy in Imperata cylindrica, a plant which accumulates large amounts of iron. The localization of ferritin was performed in epidermal, parenchymal and vascular tissues of shoots and leaves of I. cylindrica. The highest density of immunolabeling in shoots appeared in the intracellular space of cell tissues, near the cell walls and in the cytoplasm. In leaves, ferritin was detected in the proximity o... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5040774 Wed, 13 Jul 2011 23:00:00 +0100 5040774 http://www.medworm.com/index.php?rid=5040773&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21764426%26dopt%3DAbstract This study demonstrated that vasectomy causes degeneration in the seminiferous tubules with alterations in occludin distribution with a decrease in the number of spermatogenic cells. Moreover, these alterations increase in a time-dependent manner. PMID: 21764426 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5040773 Wed, 13 Jul 2011 23:00:00 +0100 5040773 http://www.medworm.com/index.php?rid=5040777&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21742368%26dopt%3DAbstract Authors: Lunedo SN, Thomaz-Soccol V, de Castro EA, Telles JE A significant increase in cutaneous leishmaniasis (CL) and its geographic expansion has motivated the development of techniques to help with diagnosis of the disease. Here we describe immunocytochemical (ICC) and immunohistochemical (IHC) techniques for the diagnosis of CL in the laboratory. Polyclonal antibodies and a modified avidin-biotin complex (Ultra Streptavidin(®)) for Leishmania (V.) braziliensis or Leishmania (L.) amazonensis were developed for the present study. In vitro culture and histological sections from experimentally infected tissues were submitted to ICC/IHC techniques. The polyclonal antibody specificity, stability and immunostaining were evaluated. The polyclonal antibodies purified by chromatography (Se... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=5040777 Fri, 08 Jul 2011 23:00:00 +0100 5040777 http://www.medworm.com/index.php?rid=4997459&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21719075%26dopt%3DAbstract In this study, we used immunofluorescent staining to assess NR1 or NR2A and PSD-95 expressions and the relationship between them in CA1, CA3, and DG of rat hippocampus on postnatal (P) days: P0, P4, P7, P10, P14, P21, P28, P56. The results showed that from P0 to P56, NR1, NR2A, and PSD-95 expressions increased gradually, and the time points of their expression peak differed in CA1, CA3, and DG during postnatal development. Interestingly, although the expression of PSD-95 was positively correlated to both NR1 and NR2A, the NR1 and PSD-95 coexpressed puncta were greatest in CA3, while NR2A and PSD-95 coexpressed puncta were greatest in CA1, compared to other subregions. Surprisingly, at P21, among different strata of CA1, the area of highest expression of NR2A was dramatically changed from s... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4997459 Tue, 28 Jun 2011 23:00:00 +0100 4997459 http://www.medworm.com/index.php?rid=4997458&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21719076%26dopt%3DAbstract Authors: Tóth S, Jonecová Z, Varga J, Staško P, Kovavalčinová B, Maretta M, Veselá J The progress of jejunal damage and recovery in the course of mesenteric ischemia-reperfusion injury in rats at different time periods was investigated. Mesenteric ischemia lasting 1h followed by 1h of reperfusion caused a significant disintegration of the mucosa, reduction of the muscular layer and diminution of the wall thickness. The loss of epithelium included enterocytes, goblet cells and Paneth cells. Paradoxically, increasing numbers of serotonin-producing cells and the beginning of regenerative processes, expressed by significantly higher proliferation, were recorded in the epithelium during this period. Disintegration of connective tissue and massive degranulation of serotonin-positive ce... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4997458 Tue, 28 Jun 2011 23:00:00 +0100 4997458 http://www.medworm.com/index.php?rid=4997460&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21705042%26dopt%3DAbstract Authors: Yang H, Lei CX, Zhang W Previous investigations on galectin-3 (gal-3) have focused mainly on its role in some malignant tumors. It was believed that gal-3 plays important roles in cell proliferation, apoptosis and adhesion in many cell types. Recently, gal-3 has been recognized as a factor related to endometrial receptivity in the human endometrium and trophoblast during embryo implantation. Human chorionic gonadotropin (hCG) is a specific embryonic hormone providing a signal from the embryo involved in preparing the receptive endometrium for embryo implantation. The current study aimed to determine whether hCG regulates gal-3 expression in endometrial cells. Our results showed that expression of gal-3 in both endometrial epithelial cells (EECs) and endometrial stromal cells (... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4997460 Thu, 23 Jun 2011 23:00:00 +0100 4997460 http://www.medworm.com/index.php?rid=4997462&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21703666%26dopt%3DAbstract Authors: Medigović I, Manojlović-Stojanoski M, Trifunović S, Ristić N, Milošević V, Zikić D, Nestorović N The effects of genistein on pituitary gonadotropic cells of immature female rats were examined and compared to actions of the synthetic estrogen, 17α-ethynylestradiol. Immature female rats received 50mg/kg/bw of genistein in dimethylsulfoxide (DMSO) subcutaneously (s.c.) daily for 3 days at 18, 19 and 20 days of age. A second group was injected with 1μg/kg of 17α-ethynylestradiol in olive oil in the same schedule. The genistein control group received DMSO only, while 17α-ethynylestradiol controls were given sterile olive oil only. Changes in cell number per mm(2), cell volume and volume density of follicle-stimulating (FSH) and luteinizing (LH) immunolabeled cells were ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4997462 Tue, 21 Jun 2011 23:00:00 +0100 4997462 http://www.medworm.com/index.php?rid=4997461&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21703667%26dopt%3DAbstract In conclusion, α-gustducin appeared in the cytoplasm of taste cells during their formation after birth, while NCAM appeared in the epithelium of the circumvallate papilla-forming area. However, these two markers of taste cells were similarly distributed within mature taste cells. PMID: 21703667 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4997461 Tue, 21 Jun 2011 23:00:00 +0100 4997461 http://www.medworm.com/index.php?rid=4997463&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21683430%26dopt%3DAbstract Authors: Serra KP, Sarian LO, Rodrigues-Peres RM, Vassallo J, Soares FA, Pinto GA, da Cunha IW, Shinzato JY, Derchain SF The aim of the study was to assess the relationship between the expression of COX-2 and p53, hormone receptors and HER-2 in the in situ (DCIS) and invasive components of ductal carcinomas (IDC) of the same breast. The expression of COX-2, p53, and hormone receptors was assessed in 87 cases of IDC with contiguous areas of DCIS. Results showed that there was no difference in COX-2 expression comparing the in situ and invasive components of the tumors. In the in situ component, there was a statistically borderline increase in p53 expression in tumors that also expressed COX-2. ER-positive specimens were more common in the group of tumors that expressed COX-2 in the inva... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4997463 Tue, 14 Jun 2011 23:00:00 +0100 4997463 http://www.medworm.com/index.php?rid=4900243&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21621827%26dopt%3DAbstract In this study we detected c-Myc expression pattern in the process of mouse hair follicle development and normal cycle. We found that during hair follicle morphogenesis, the stage-specific expression of c-Myc was detected in mouse skin and was predominantly localized to the hair follicle epithelium. c-Myc expression was also consistently found in mouse skin throughout the hair follicle cycle. Through the in vivo injection of c-Myc inhibitory peptide and c-Myc expression plasmid, we also investigated the direct effects of c-Myc on the hair follicle structures during the hair follicle cycle. Our results showed that c-Myc inhibitory peptide significantly restrained the development of anagen hair follicles, while the injection of plasmid DNA encoding c-Myc in vivo clearly promoted anagen develo... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4900243 Thu, 26 May 2011 23:00:00 +0100 4900243 http://www.medworm.com/index.php?rid=4900242&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21621828%26dopt%3DAbstract In conclusion, these findings suggest that the seminal vesicle and prostate gland could be the possible sources of leptin in the seminal plasma. This leptin might have a direct (paracrine, autocrine or both) effect on epithelial cells of the accessory male genital glands, on the spermatozoa via spermatozoan leptin receptors. PMID: 21621828 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4900242 Thu, 26 May 2011 23:00:00 +0100 4900242 http://www.medworm.com/index.php?rid=4900246&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21620444%26dopt%3DAbstract Authors: Wang XT, Zhou CL, Yan JL, Yan X, Xie HX, Sun CL The aim of the study was to investigate the fate of donor osteocytes in fine particulate bone powders during repair of bone defects in experimental rats. The iliac bone of male inbred DA rats was harvested and used as the larger bone grafts and also prepared as fine particulate (granulated) bone powders (300-500μm size particles) for transplantation into radial defects in female rats. The presence and relative amounts of genes specific to the sex-determining region of the Y-chromosome (Sry) originating from the bone grafts were evaluated by polymerase chain reaction and by in situ hybridization, respectively. Additional samples were evaluated histologically. In the larger bone grafts, the expression of Sry decreased relatively e... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4900246 Wed, 25 May 2011 23:00:00 +0100 4900246 http://www.medworm.com/index.php?rid=4900244&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21621253%26dopt%3DAbstract Authors: Rashed RM, El-Alfy SH Extraocular muscles (EOMs) are unique as they show greater variation in anatomical and physiological properties than any other skeletal muscles. To investigate the muscle fiber types and to understand better the structure-function correlation of the extraocular muscles, the present study examined the ultrastructural characteristics of the superior rectus muscle of rat. The superior rectus muscle is organized into two layers: a central global layer of mainly large-diameter fibers and an outer C-shaped orbital layer of principally small-diameter fibers. Six morphologically distinct fiber types were identified within the superior rectus muscle. Four muscle fiber types, three single innervated fibers (SIFs) and one multiple innervated fiber (MIF), were recogn... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4900244 Wed, 25 May 2011 23:00:00 +0100 4900244 http://www.medworm.com/index.php?rid=4900245&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21620445%26dopt%3DAbstract Authors: Galas J, Słomczyńska M, Knapczyk-Stwora K, Durlej M, Starowicz A, Tabarowski Z, Rutka K, Szołtys M Immunoexpression of 3β-hydroxysteroid dehydrogenase (3β-HSD), cytochrome P450c17 (P450c17), androgen receptor (AR), and steroid contents were studied in the ovaries of immature female Wistar rats killed between postnatal days 1 and 30. During days 1-7, ovarian somatic structures lacked AR, 3β-HSD and P450c17, except for the surface epithelium, which featured the presence of these three proteins, suggestive of its androgen responsiveness and steroidogenic function. On day 10, AR appeared in many somatic structures, including the granulosa layers, which coincided with the P450c17 immunoexpression in some theca/interstitial cells, and an increase in ovarian androgen concentrat... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4900245 Tue, 24 May 2011 23:00:00 +0100 4900245 http://www.medworm.com/index.php?rid=4852177&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21570708%26dopt%3DAbstract We describe a modified PVA-based cryoembedding medium, composed of PVA (10wt% and 15wt%) with the addition of boric acid (from 0 to 5wt%), that can improve the sectioning properties and efficiency of frozen tissue for histological analysis. The amount of load under the same compressive displacement as well as cohesive force increased with increasing boric acid and PVA contents. 15wt% PVA and 3wt% boric acid was determined as an optimal composition for cryoembedding material based on the sectioning efficiency measured by the numbers of unimpaired sectioned slices and the amount of load under the same compressive displacement test. On the basis of the results of routine hematoxylin and eosin staining of cryosections of tissue embedded in a medium with 3wt% boric acid and PVA, it was conclude... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4852177 Wed, 11 May 2011 23:00:00 +0100 4852177 http://www.medworm.com/index.php?rid=4852179&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21565387%26dopt%3DAbstract Authors: Toledo-Corral CM, Banner LR Peripheral neuropathy is a common complication of diabetes mediated by alterations of growth factors. Members of the neuropoietic cytokine family, which include IL-6, LIF, and CNTF among others, have been shown to be important regulators of peripheral nerves and the muscles that they innervate. To investigate their potential role in diabetic nerve and muscle, we studied the expression of the shared receptor subunits, LIFR and gp130 in a mouse model of streptozotocin (STZ)-induced diabetes. The results of Western blotting and densitometric analysis showed that both LIFR and gp130 protein expression were increased in diabetic sciatic nerve compared to control mice at early time points following STZ injection. In diabetic gastrocnemius muscle, LIFR and... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4852179 Mon, 09 May 2011 23:00:00 +0100 4852179 http://www.medworm.com/index.php?rid=4852178&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21565388%26dopt%3DAbstract This study analyzed the morphological localization and abundance of mast cells in the intestine and gills of sea bream, Sparus aurata, after a 12, 24 or 72h exposure to PCB 126, a polychlorinated biphenyl, which is a potent immunotoxic agent. In the organs of fish exposed to PCB 126, it was observed that in addition to congestion of blood vessels, there was extravasation of red blood cells, infiltration of lymphocytes, and a progressive increase in numbers of mast cells. These data confirm the immunotoxic action of PCB, and the involvement of mast cells in the inflammatory response. PMID: 21565388 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4852178 Mon, 09 May 2011 23:00:00 +0100 4852178 http://www.medworm.com/index.php?rid=4852180&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21549414%26dopt%3DAbstract Authors: Yin Z, Zhang Y, Li Y, Lv T, Liu J, Wang X Activation of signal transducer and activator of transcription 3 (STAT3) plays important roles in tumorigenesis and tumor development. Previously, we have reported that overexpression of STAT3 potentiates growth, survival and radioresistance of non-small cell lung cancer (NSCLC) cells. The aim of this study was to investigate the prognostic significance of STAT3 expression and its correlation with chemoresistance of NSCLC cells. Semi-quantitative RT-PCR was performed to detect the expression of STAT3 mRNA in 12 NSCLC and corresponding adjacent lung tissues. Immunohistochemistry was performed to detect the expression of STAT3 protein in 76 NSCLC tissue samples. Additionally, the correlation between STAT3 expression and prognosis of NSCL... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4852180 Tue, 03 May 2011 23:00:00 +0100 4852180 http://www.medworm.com/index.php?rid=4798381&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21531010%26dopt%3DAbstract Authors: Okuthe GE, Fabian BC Progression through mitosis and meiosis during early zebrafish ovarian development is accompanied by highly regulated series of transformations in the architecture of oocytes. These cytoskeletal-dependent membrane events may be assumed to be brought about by deployment of proteins. While the cytoskeleton and its associated proteins play a pivotal role in each of these developmental transitions, it remains unclear how specific cytoskeletal proteins participate in regulating diverse processes of oocyte development in zebrafish. Results from this study show that a pool of spectrin accumulates during oogenesis and parallels an increase in volume of oocytes at pre-vitellogenic stages of development. Spectrin labeling is restricted to the surface of oogonia, the... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4798381 Wed, 27 Apr 2011 23:00:00 +0100 4798381 http://www.medworm.com/index.php?rid=4798382&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21529898%26dopt%3DAbstract Authors: Frisch KE, Duenwald-Kuehl SE, Kobayashi H, Chamberlain CS, Lakes RS, Vanderby R Collagen fibers and fibrils that comprise tendons and ligaments are disrupted or damaged during injury. Fibrillogenesis during healing produces a matrix that is initially quite disorganized, but remodels over time to resemble, but not replicate, the original roughly parallel microstructure. Quantification of these changes is traditionally a laborious and subjective task. In this work we applied two automated techniques, fast Fourier transformation (FFT) and fractal dimension analysis (FA) to quantify the organization of collagen fibers or fibrils. Using multi-photon images of collagen fibers obtained from rat ligament we showed that for healing ligaments, FA differentiates more clearly between the ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4798382 Tue, 26 Apr 2011 23:00:00 +0100 4798382 http://www.medworm.com/index.php?rid=4798383&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21524787%26dopt%3DAbstract Authors: Meyer W, Schmidt J, Busche R, Jacob R, Naim HY The sensitive red fluorescence dye BODIPY® 665/676, and embedding in the water-soluble resin Technovit® 7100 were used to demonstrate free fatty acids in the epidermis of seven semi-aquatic and aquatic mammalian species with a sparse or dense hair coat. The staining generally marked lipid layers of varying thickness between the lamellae of the Stratum corneum, as found particularly in very densely haired species (otter), but also in rather sparsely haired animals (beaver, nutria), and especially in the seal. The very sparsely haired capybara contained no free fatty acids in the corneal layer system, but exhibited an accumulation of such substances in the vital epidermis. All haired species showed a strongly positive reaction sta... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4798383 Sun, 24 Apr 2011 23:00:00 +0100 4798383 http://www.medworm.com/index.php?rid=4798384&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21507466%26dopt%3DAbstract In this study, we demonstrate, by example of Vestimentiferan tubeworms (Siboglinidae, Polychaeta), that the simultaneous fluorescence in situ hybridization (FISH) of both eukaryotic host and bacterial symbiont cells is possible on a single semi-thin (1μm) section. This allows the identification of host specimens to species level as well as offering visualization of bacteria distributed within the host tissue. Previously published 18S rRNA host-specific oligonucleotide probes for Riftia pachyptila, Tevnia jerichonana and a newly designed Oasisia alvinae probe, as well as a 16S rRNA probe targeting symbionts found in all host species, were applied. A number of standard fixation and hybridization parameters were tested and optimized for the best possible signal intensity and cellular resolut... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4798384 Sun, 17 Apr 2011 23:00:00 +0100 4798384 http://www.medworm.com/index.php?rid=4798386&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21501858%26dopt%3DAbstract Authors: Rambaruth ND, Dwek MV The development of secondary cancers, metastases, requires that a multitude of events are completed in an ordered and sequential manner. This review focuses on the role of cell surface glycans and their binding partners in the metastatic process. A common feature of metastasis is that the steps require adhesive interactions; many of these are mediated by cell surface glycans and their interactions with endogenous carbohydrate binding proteins (lectins). Aberrant glycosylation is a key feature of malignant transformation and the glycans involved influence the adhesive interactions of cancer cells often providing favorable conditions for tumor dissemination. This review focuses on glycans on the cancer cell surface and their association with endogenous lect... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4798386 Fri, 15 Apr 2011 23:00:00 +0100 4798386 http://www.medworm.com/index.php?rid=4798385&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21501859%26dopt%3DAbstract Authors: Narita D, Seclaman E, Ursoniu S, Anghel A ADAMs (a desintegrin and metalloprotease) are transmembrane glycoproteins involved in cell growth, differentiation, motility, and respectively, tumor growth and progression. Our aim was to evaluate ADAM12 spliced variants (ADAM12L - long membrane-bound and ADAM12S - secreted-short variant) and ADAM17 genes expression in breast cancers and to correlate their level of expression with clinical and pathological characteristics. Expression of ADAMs was analyzed using quantitative reverse-transcription polymerase chain reaction in laser-capture microdissected specimens of breast cancers and corresponding non-neoplastic breast tissues from 92 patients. The proteins' expression was confirmed by immunohistochemistry. Significantly elevated amou... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4798385 Fri, 15 Apr 2011 23:00:00 +0100 4798385 http://www.medworm.com/index.php?rid=4798387&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21489608%26dopt%3DAbstract This study reveals, for the first time, that prolactin mRNA was expressed in extra-pituitary brain areas in birds. In addition, the diverse expression of prolactin mRNA in the brain areas suggests that prolactin plays various physiological roles in birds. PMID: 21489608 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4798387 Sun, 10 Apr 2011 23:00:00 +0100 4798387 http://www.medworm.com/index.php?rid=4798389&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21477847%26dopt%3DAbstract Authors: Krieg R, Eitner A, Halbhuber KJ Homo-dimers of styryl dyes were chemically tailored in order to become specific cytochemical probes for use in the life sciences. Histochemical applications using fixed cryotome sections are discussed. It is concluded, that homo-dimerization of specific styryl substrates of peroxidase (PO) by way of their covalent linkage, does not necessarily lead to improved detection sensitivity of endogenous and immuno-bound peroxidase (PO) activity. In general, these dimers act less specific towards PO activity than parent monomers. Synergetic interactions of the doubled basic dye compartments with cell constituents cause a pronounced staining of further targets at the cellular level. This behavior depends on the functional groups present in each dye compar... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4798389 Wed, 06 Apr 2011 23:00:00 +0100 4798389 http://www.medworm.com/index.php?rid=4798388&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21477848%26dopt%3DAbstract Authors: Zachar PC, Jonz MG The oropharyngeal cavity in fish supports a range of sensory modalities, including detection of chemical and mechanical stimuli. Taste buds are found throughout this tissue and may participate in both processes. We used confocal microscopy and immunohistochemistry to characterize the morphology of Merkel-like cells and their association with other cell types and nerve fibers of the taste bud in the vertebrate model, the zebrafish. In addition, we document procedures for the observation of these structures in whole-tissue preparations from larvae and adults using zebrafish-specific and monoclonal antibodies. A single microvillus Merkel-like cell was found in each taste bud regardless of age or location. Merkel-like cells were neurosecretory, as indicated by l... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4798388 Tue, 05 Apr 2011 23:00:00 +0100 4798388 http://www.medworm.com/index.php?rid=4693660&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474164%26dopt%3DAbstract This study provides indications that apigenin and lycopene, which are considered as chemopreventive agents, to be effective in vitro on endothelial cells and angiogenesis. PMID: 21474164 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4693660 Mon, 04 Apr 2011 23:00:00 +0100 4693660 http://www.medworm.com/index.php?rid=4693659&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21474165%26dopt%3DAbstract Authors: Jeong C, Shin T In order to investigate the expression of protein kinase C (PKC) beta I in the retinas of pigs during postnatal development, we analyzed retinas sampled from 3-day-old and 6-month-old pigs by Western blotting and immunohistochemistry. Western blot analysis detected the expression of PKC beta I in the retinas of 3-day-old piglets and it was increased significantly in the retinas of 6-month-old adult pigs. Immunohistochemical staining showed PKC beta I in the retinas of both groups. Immunohistochemistry of 3-day-old retinas revealed weak PKC beta I reactivity in the ganglion cell layer, inner plexiform layer, inner nuclear cell layer, outer plexiform layer and rod and cone cell layer. In the 6-month-old pig retina, the cellular localization of PKC beta I immunost... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4693659 Mon, 04 Apr 2011 23:00:00 +0100 4693659 http://www.medworm.com/index.php?rid=4693661&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21466889%26dopt%3DAbstract Authors: Cayli S, Eyibilen A, Gurbuzler L, Koc S, Atay GA, Ekici A, Aladağ I Jab1, which is a fifth component of COP9 signalosome, plays an essential role in cell growth and proliferation. Jab1 is also shown to regulate transforming growth factor-beta (TGF-β) signaling in carcinoma cells. The aim of the present study was to investigate the expression and the correlation of Jab1 and TGF-β1 in chronic rhinosinusitis and nasal polyposis. Here, we show the elevated expression of Jab1 and TGF-β1 in diseased mucosa without nasal polyps and a correlation between Jab1 and TGF-β1 expression. Forty-six samples (26 patients with nasal polyps, 10 patients with chronic rhinosinusitis and 10 control subjects) were included to this study. Immunohistochemistry and Western blotting were performed ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4693661 Sat, 02 Apr 2011 23:00:00 +0100 4693661 http://www.medworm.com/index.php?rid=4693663&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21439611%26dopt%3DAbstract Authors: Wiwanitkit V PMID: 21439611 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4693663 Wed, 23 Mar 2011 00:00:00 +0100 4693663 http://www.medworm.com/index.php?rid=4693662&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21439612%26dopt%3DAbstract Authors: Alkafafy M, Rashed R, Helal A The study aimed to evaluate the validity of immunohistochemistry in the differential labeling of the diverse components of the lactating mammary gland. Paraffin-embedded sections of lactating bovine mammary glands were stained by conventional and histochemical techniques. Primary antibodies against S100, alpha smooth muscle actin (α-SMA), connexin-43 (Cx43), cytokeratin-14 (Ck14), galactosyltransferase (GalTase), angiotensin converting enzyme (ACE) and vascular endothelial growth factor (VEGF) were applied on paraffin sections. Strong cytoplasmic and nuclear S100 immunoreactivity was mainly expressed by alveolar epithelium and to a lesser variable extent by ductal epithelium. The Golgi zone of the epithelial cells expressed strong GalTase immunos... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4693662 Wed, 23 Mar 2011 00:00:00 +0100 4693662 http://www.medworm.com/index.php?rid=4636421&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21414652%26dopt%3DAbstract Authors: Mercati F, Dall'aglio C, Pascucci L, Boiti C, Ceccarelli P In veterinary medicine, there is an increasing interest in the study of the endo-cannabinoid system and the possible use of the cannabinoids for the treatment of several diseases. Cannabinoid receptors (CB) are widely distributed in human and laboratory animal tissues, justifying the involvement of the endo-cannabinoid system in a great number of metabolic ways. Since there are no data regarding cannabinoid receptors in hair follicles of domestic animals, we investigated the presence and localization of CB1 receptor in dog hair follicles. By using a goat anti-CB1 polyclonal antibody, we observed CB1 receptor in the proximal part of both primary and secondary hair follicles. Staining was localized in the inner root shea... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4636421 Tue, 15 Mar 2011 00:00:00 +0100 4636421 http://www.medworm.com/index.php?rid=4636423&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21411123%26dopt%3DAbstract In this study, we show that the unicellular cyanobacterial species Synechococcus sp. IU 625 adapts to growth in the presence of mercuric chloride, recovering from pigmentation and morphological defects. Cells accumulate mercury within 2h of growth and by 3 days, the total mercury concentration is significantly reduced, with all remaining mercury associated with the cells. This suggests that Synechococcus sp. IU 625 can convert mercury to a volatile form. PMID: 21411123 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4636423 Mon, 14 Mar 2011 00:00:00 +0100 4636423 http://www.medworm.com/index.php?rid=4636422&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21411124%26dopt%3DAbstract Authors: Wirakiat W, Udomuksorn W, Vongvatcharanon S, Vongvatcharanon U The study investigated the effects of estrogen on parvalbumin (PV) levels in cardiac myocytes of ovariectomized rats, which is a model system for postmenopausal woman. Parvalbumin acts as a relaxing factor in cardiac myocytes. Adult female Wistar rats, 12 weeks old, were randomly divided into 5 groups of 10: sham-operated (SHAM), ovariectomized (OVX), and OVX receiving estrogen replacement of 10μg/kg (Es10), 20μg/kg (Es20) and 40μg/kg (Es40). After 10 weeks, serum estrogen levels were measured and the α and β estrogen receptors in cardiac myocytes were investigated by immunohistochemistry. PV levels were examined by immunohistochemistry and Western blot analysis. Cardiac myocytes of all animals showed strong s... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4636422 Mon, 14 Mar 2011 00:00:00 +0100 4636422 http://www.medworm.com/index.php?rid=4636427&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21397932%26dopt%3DAbstract Authors: Li Y, Zhang C The ecotropic viral integration site-1 (Evi-1) is a transcription factor with two sets of zinc finger domains. It is an important regulator of the transforming growth factor beta superfamily. In the present study, we investigated the spatiotemporal expression patterns of Evi-1 using immunohistochemistry in ovaries from neonatal mice, gonadotropin-induced immature mice and mice in estrous cycle. Positive staining for Evi-1 was detected in the cytoplasm of oocytes. In postnatal mice, a high level of Evi-1 immunostaining was found from day 1 to 6, an intermediate level from day 10 to 16 and a low level on day 23. After gonadotropin treatment, Evi-1 was mainly expressed in small follicles and exhibited a very low level in large antral follicles. During the estrous cy... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4636427 Fri, 11 Mar 2011 00:00:00 +0100 4636427 http://www.medworm.com/index.php?rid=4636426&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21397933%26dopt%3DAbstract In this study we examined the process and the matrix components of the calcified tissues by means of subcutaneously transplanted dental pulp tissue. The mid-third of the mouse incisor pulp was transplanted into abdominal subcutaneous tissue. Two calcified tissues were independently formed within the implanted pulp at 7days after the implantation, one developed in the peripheral region and the other was formed in the middle region of the pulp. Histological investigation indicated the existence of hypertrophic chondrocytes in the peripheral calcified tissue. Immunohistochemical study indicated the colocalization of types I and II collagen surrounding these cells. RT-PCR analysis indicated the transient expression of type II collagen at 7days and the constant expression of type I collagen, os... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4636426 Fri, 11 Mar 2011 00:00:00 +0100 4636426 http://www.medworm.com/index.php?rid=4636425&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21397934%26dopt%3DAbstract Authors: Dall'aglio C, Pascucci L, Mercati F, Boiti C, Ceccarelli P The aim of the present study was to investigate by immunohistochemistry the presence and distribution of the orexin system in the stomach and gut of fallow deer. Abundant orexin A-positive cells were localized in the middle and basal portions of the mucosal glands of the cardial and fundic regions of the stomach. In the same gastric areas, orexin B-positive cells were also found, mainly localized in the basal portion of glands. In the intestinal tract, orexin-containing cells were occasionally found in the duodenal epithelium and in the rectal intestinal glands. Immunoreactivity for orexin receptors, type 1 and 2 (OX1R and OX2R), was not detected in the same stomach regions. OX1R-immunopositivity was observed in the en... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4636425 Fri, 11 Mar 2011 00:00:00 +0100 4636425 http://www.medworm.com/index.php?rid=4636424&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21397935%26dopt%3DAbstract Authors: Zaccone D, Gopesh A, Anastasi G, Favaloro A, Sfacteria A, Marino F The carotid labyrinth is an enigmatic endocrine structure of unknown chemosensory function lying in the gill region of the catfishes. The carotid body is found at the carotid bifurcation of amphibians and all mammalian vertebrates on the evolutionary tree. It is a vascular expansion comprised of a cluster of glomus cells with associated (afferent and efferent) innervations. In the catfish species studied (Clarias batrachus) a neurosecretory cell system consisting of pseudobranchial neurosecretory cells connect the carotid labyrinth or large vessels (both the efferent branchial artery and dorsal aorta), and is likely akin to the glomus cells, but comparing these structures in widely divergent vertebrate species,... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4636424 Fri, 11 Mar 2011 00:00:00 +0100 4636424 http://www.medworm.com/index.php?rid=4636428&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21397298%26dopt%3DAbstract Authors: Kaneko Y, Fujimaki-Aoba K, Watanabe SI, Hokari S, Takada M Adult amphibian skin has served as a model for the investigation of Na(+)-transporting epithelia, such as mammalian renal tubules. The amiloride-blockable epithelial Na(+) channel (ENaC), which is located in the apical membrane of the outer living cell layer, regulates Na(+) transport across the epithelium. ENaC is thought to develop during the terminal differentiation of epidermal cells, but the details are unclear. Here, we used in situ hybridization to examine the localization of the ENaC subunit mRNAs in skin of adult bullfrogs, to clarify the development of ENaC. We found that α-ENaC mRNA was expressed within the cells of the Stratum granulosum, the Stratum spinosum, and the Stratum germinativum, while β-ENaC mR... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4636428 Thu, 10 Mar 2011 00:00:00 +0100 4636428 http://www.medworm.com/index.php?rid=4636429&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21392819%26dopt%3DAbstract This study was undertaken to investigate the chronic effects of lead exposure on the serotoninergic system in the dorsal raphe nucleus (DRN) and the consequences of its toxicity on rat behavior. Adult male Wistar rats were chronically exposed for 3 months to 0.5% lead acetate in drinking water. The serotoninergic system was evaluated using immunohistochemistry and the anxiety behavior was assessed by the light/dark box test. The results show that chronic lead exposure induces a significant increase of blood and brain lead levels in treated rats compared with controls. The density of the immunoreactive serotoninergic cell bodies was significantly higher in treated rats in all parts of the DRN. Assessment of animal behavior using the light/dark box test showed that lead-treated rats spent si... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4636429 Tue, 08 Mar 2011 00:00:00 +0100 4636429 http://www.medworm.com/index.php?rid=4573880&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21376374%26dopt%3DAbstract This study investigated the developmental changes of glycoconjugate patterns in the porcine vomeronasal organs (VNOs) and associated glands (Jacobson's glands) from prenatal (9 weeks of gestation) and postnatal (2 days after birth) to the sexually mature stage (6 months old). The VNO of pigs (Sus scrofa) was examined using the following: Dolichos biflorus agglutinin (DBA), Bandeiraea simplicifolia agglutinin isolectin B4 (BSI-B4), Triticum vulgaris agglutinin (WGA), Ulex europaeus agglutinin I (UEA-I), and soybean agglutinin (SBA). At the fetal stage, all lectins examined were detected mainly in the free border of the vomeronasal epithelium, but few (WGA and UEA-I) and or absent in the VNO cell bodies. At the postnatal and sexually mature stages, the reactivity of some lectins, including W... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4573880 Wed, 02 Mar 2011 00:00:00 +0100 4573880 http://www.medworm.com/index.php?rid=4573878&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21376375%26dopt%3DAbstract Authors: Allon I, Aballo S, Dayan D, Vered M We conducted a comprehensive study of all lipomatous tumors of the oral mucosa (1996-2008) accessioned at the Department of Oral Pathology, Tel Aviv University, collected demographic data and analyzed multiple histomorphological features. Furthermore, we examined the immunostaining of aP2 (adipocyte lipid binding protein) and the polarization colors of picrosirius red (PSR)-stained collagen fibers in order to test their potential in differentiating between benign and malignant tumors. All cases were immunohistochemically stained with aP2 antibody; only tumors with considerable collagenous stroma were selected for the PSR staining. A total of 77 tumors were included in the study, 91% benign and 9% malignant. Fibrolipoma (37.7%) and lipoma (36... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4573878 Wed, 02 Mar 2011 00:00:00 +0100 4573878 http://www.medworm.com/index.php?rid=4573883&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21371741%26dopt%3DAbstract In conclusion, our data show that the cell populations in the placenta stain for a number of cell cycle related proteins and that these staining patterns change as a function of both gestational age and abnormal placentation. PMID: 21371741 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4573883 Tue, 01 Mar 2011 00:00:00 +0100 4573883 http://www.medworm.com/index.php?rid=4573884&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21367445%26dopt%3DAbstract Re: Erbüyün K, et al. "Effects of levosimendan and dobutamine on experimental acute lung injury in rats" [Acta Histochem 111 (2009) 404-414]. Acta Histochem. 2011 Feb 28; Authors: Erbüyün K PMID: 21367445 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4573884 Mon, 28 Feb 2011 00:00:00 +0100 4573884 http://www.medworm.com/index.php?rid=4518736&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21306758%26dopt%3DAbstract Authors: Zhang XJ, Liu XM PMID: 21306758 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4518736 Mon, 07 Feb 2011 00:00:00 +0100 4518736 http://www.medworm.com/index.php?rid=4518737&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21277008%26dopt%3DAbstract In this study, reverse transcription-PCR assay was performed to detect the expression of Bcl-xL mRNA in CRC and corresponding non-tumor colon tissues. Immunohistochemistry was performed to detect the immunolocalization of Bcl-xL protein in sixty-eight primary CRC tissue samples. The association between Bcl-xL protein expression and clinicopathological factors of CRC patients was analyzed and the survival was assessed by the Kaplan-Meier method and proportional hazards model. The averaged level of Bcl-xL mRNA expression in CRC tissues (0.85±0.13) was significantly higher than that in non-tumor colon tissues (0.08±0.02). Immunohistochemical staining showed that the Bcl-xL protein was mainly located in the cytoplasm of tumor cells. The level of Bcl-xL protein expression was closely correlat... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4518737 Fri, 28 Jan 2011 00:00:00 +0100 4518737 http://www.medworm.com/index.php?rid=4518738&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21272924%26dopt%3DAbstract In conclusion, overexpression of Thy1 may not suppress the development of HCC. Thy1 could provide a clinical prognostic marker for HCC. PMID: 21272924 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4518738 Tue, 25 Jan 2011 00:00:00 +0100 4518738 http://www.medworm.com/index.php?rid=4518739&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21269661%26dopt%3DAbstract Authors: Tunçdemir M, Oztürk M The aim of the study was to determine in a rat model of streptozotocin-induced diabetic nephropathy the expression of: WT-1 (for podocyte loss in the glomerulus), TGF-beta 1 (for tissue damage), caspase-3 and bax (for glomerular apoptosis) and the possible protective effects of an angiotensin II type 1 receptor blocker. Three groups of male Wistar albino rats were used. The first group consisted of non-diabetic control rats. The second group was the untreated diabetic rats. The third group consisted of diabetic rats treated with Irbesartan, which is an angiotensin II receptor antagonist, widely used in treatment for hypertension. Immunohistochemical stainings for TGF-beta 1, bax, caspase-3 and WT-1 were performed. The microalbuminuria levels of the Irbe... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4518739 Mon, 24 Jan 2011 00:00:00 +0100 4518739 http://www.medworm.com/index.php?rid=4317269&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21195462%26dopt%3DAbstract Authors: Mahapatra C, Mahapatra PK Cathepsin D, an aspartyl protease, plays a key role in the metabolic degradation of intracellular proteins in an acidic milieu of lysosomes. Proteolysis plays an essential role in anuran tail regression and a wide variety of thyroid hormone induced proteolytic enzymes have been reported to be involved in the regressing tail. The present study describes the trend of specific activity of cathepsin D in the tail of different developmental stages and immunohistochemical localization of cathepsin D during degradation of various tail tissues in the tadpoles of Polypedates maculatus. Cathepsin D has been found to be involved in the degradation of major tail tissues such as epidermis, muscle, spinal cord, notochord cells and blood cells in the regressing tail... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4317269 Thu, 30 Dec 2010 00:00:00 +0100 4317269 http://www.medworm.com/index.php?rid=4317270&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21194732%26dopt%3DAbstract In this study we have analyzed the ultrastructural distribution of the MUC5B mucin in human palatal and buccal glands by means of post-embedding immunoelectron microscopy. Thin sections of normal human buccal and palatal glands obtained at surgery, were treated with polyclonal antibodies to human salivary MUC5B. Intense MUC5B reactivity was observed in the secretory granules of mucous cells of all glands examined. The present results provide new data regarding the secretory pattern of MUC5B in human buccal and palatal glands, indicating their significant contribution to the maintenance of the mucous biofilm that protects buccal and palatal mucosal areas. PMID: 21194732 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4317270 Wed, 29 Dec 2010 00:00:00 +0100 4317270 http://www.medworm.com/index.php?rid=4317271&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21193226%26dopt%3DAbstract Authors: Gunčová I, Látr I, Mazurová Y Although Huntington's disease (HD) occurs only in humans, the use of animal models is crucial for HD research. New genetic models may provide novel insights into HD pathogenesis, but their relevance to human HD is problematic, particularly owing to a lower number of typically degenerated and dying striatal neurons and consequent insignificant reactive gliosis. Hence, neurotoxin-induced animal models are widely used for histopathological studies. Unlike in humans, the neurodegenerative process (NDP) of the HD phenotype develops very fast after the application of quinolinic acid (QA). For that reason, we compared three groups of rats in more advanced stages (1-12 months) of the QA lesion with 3 representative HD cases of varying length and grade... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4317271 Tue, 28 Dec 2010 00:00:00 +0100 4317271 http://www.medworm.com/index.php?rid=4317272&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21168900%26dopt%3DAbstract In this study, sections of paraffin-embedded tumor adjacent lung tissue and sections of adenocarcinoma of the lung, squamous cell carcinoma of the lung and metastases of colonic adenocarcinoma were investigated for the distribution and expression of Gd using a polyclonal anti-Gd antibody. Glycodelin protein is located in the cytoplasm of bronchial epithelial cells, pneumocytes and alveolar macrophages. Furthermore, Gd is expressed in adenocarcinoma and squamous cell carcinoma of the lung as well as in lung metastases of colonic adenocarcinoma. Densitometric analyses showed a significantly increased expression of glycodelin protein in cancer tissue compared to tumor adjacent lung tissue. The Gd protein level was 1.7-2.6-fold increased in lung carcinoma compared to tumor adjacent lung tissue... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4317272 Fri, 17 Dec 2010 00:00:00 +0100 4317272 http://www.medworm.com/index.php?rid=4317273&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21168191%26dopt%3DAbstract Authors: Azman J, Starcevic Klasan G, Ivanac D, Picard A, Jurisic-Erzen D, Nikolic M, Malnar D, Arbanas J, Jerkovic R The Reg IV gene has been documented in the human colon, small intestine, stomach and pancreas. Expression of the Reg IV in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. Since the distribution of the Reg IV protein in normal rat tissues is unknown, the aim of this study was to reveal the expression of the Reg IV protein in structurally and functionally different rat organs. The expression of Reg IV gene was analyzed by Western blot and reverse transcription-polymerase chain reaction. Immunohistochemistry was used to localize Reg IV protein. Reg IV protein was expressed in pancreas, st... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4317273 Thu, 16 Dec 2010 00:00:00 +0100 4317273 http://www.medworm.com/index.php?rid=4271952&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21156321%26dopt%3DAbstract In conclusion, our study demonstrated that GRP78 may play some important roles in the development and progression of colorectal carcinomas. The expression of GRP78 is associated with the enhanced proliferation of colorectal carcinoma cells and with their resistance to apoptosis. PMID: 21156321 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4271952 Sun, 12 Dec 2010 00:00:00 +0100 4271952 http://www.medworm.com/index.php?rid=4271953&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21146857%26dopt%3DAbstract Authors: Zhao L, He J, Guo Q, Wen X, Zhang X, Dong C Oocyte-secreted growth differentiation factor (GDF) 9 plays an essential role during follicle maturation through actions on granulosa cells. Despite its critical role in female reproduction, GDF9 expression, signalling and function are less well characterized during spermatogenesis. The purpose of this study was to investigate temporal and spatial expression and potential cellular targets of GDF9 in the adult cat testis. Our result confirmed that GDF9 is stage-specifically localized in the cytoplasm of round spermatids and pachytene spermatocytes of the cat seminiferous epithelium. In particular, activin receptor-like kinase (ALK) 5, the type I receptor of GDF9, is principally localized in the cytoplasm of round spermatids. Smad2/3, ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4271953 Fri, 10 Dec 2010 00:00:00 +0100 4271953 http://www.medworm.com/index.php?rid=4271955&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21145575%26dopt%3DAbstract Authors: Capková L, Dávidová A, Pavel J, Kucharíková A, Radoňak J, Kuchárová K, Cigánková V, Maršala M, Lukáčová N Guanylyl cyclase (GC) as the effector molecule for nitric oxide (NO) plays a key role in the NO/cGMP signalling cascade. Based on these observations, our study focused on NO/sGC signalization in the bulbospinal respiratory pathway. The distribution of neuronal nitric oxide synthase (nNOS), β1 subunit of soluble guanylyl cyclase (β1sGC) and synaptophysin (SYN) was explored in the upper part of the respiratory pathway after C2-C3 hemisection of the spinal cord in male Wistar rats. Unilateral injection of Fluorogold into the phrenic nucleus (PN) at C4 level and survival of animals for 2 days revealed many Fluorogold fluorescent neurons in the ventral respirato... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4271955 Wed, 08 Dec 2010 00:00:00 +0100 4271955 http://www.medworm.com/index.php?rid=4271954&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21145576%26dopt%3DAbstract Authors: Henson SE, Morford T, Stein MP, Wall R, Malone CS Mantle cell lymphoma and small lymphocytic lymphoma are lymphocyte cancers that have similar morphologies and a common age of onset. Mantle cell lymphoma is generally an aggressive B cell lymphoma with a short median survival time, whereas small lymphocytic lymphoma is typically an indolent B cell lymphoma with a prolonged median survival time. Using primary tumor samples in bi-directional suppression subtractive hybridization, we identified genes with differential expression in an aggressive mantle cell lymphoma versus an indolent small lymphocytic lymphoma. "Virtual" Northern blot analyses of multiple lymphoma samples confirmed that a set of genes was preferentially expressed in aggressive mantle cell lymphoma compared to ind... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4271954 Wed, 08 Dec 2010 00:00:00 +0100 4271954 http://www.medworm.com/index.php?rid=4247648&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21131025%26dopt%3DAbstract Authors: Bardales JR, Cascallana JL, Villamarín A The cAMP signalling pathway is involved in the regulation of basic physiological processes in bivalve molluscs. We had previously identified and characterized two isoforms of cAMP-dependent protein kinase (PKA) from the sea mussel Mytilus galloprovincialis that differ at their regulatory (R) subunit, namely, R(myt1) or R(myt2). Here we investigated the immunohistochemical expression of both PKA isoforms in various mussel tissues. R(myt1) and R(myt2) displayed a complementary subcellular localization. In general, R(myt1) was found to be uniformly distributed in the cytoplasm of most cell types, whereas R(myt2) appears to be localized only in the cell periphery and associated with certain cellular structures, such as the cilia of labial ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4247648 Fri, 03 Dec 2010 00:00:00 +0100 4247648 http://www.medworm.com/index.php?rid=4247647&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21131026%26dopt%3DAbstract Authors: Simons PJ, Boon L PMID: 21131026 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4247647 Fri, 03 Dec 2010 00:00:00 +0100 4247647 http://www.medworm.com/index.php?rid=4164418&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21055795%26dopt%3DAbstract Authors: Coleman R PMID: 21055795 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4164418 Wed, 03 Nov 2010 00:00:00 +0100 4164418 http://www.medworm.com/index.php?rid=4136058&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21047672%26dopt%3DAbstract Authors: Yang JJ, Ji MH PMID: 21047672 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4136058 Mon, 01 Nov 2010 00:00:00 +0100 4136058 http://www.medworm.com/index.php?rid=4107172&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20950843%26dopt%3DAbstract Authors: Orendáčová J, Orendáč M, Mojžiš M, Labun J, Martončíková M, Saganová K, Lievajová K, Blaško J, Abdiová H, Gálik J, Račeková E The immediate effects of whole body electromagnetic radiation (EMR) were used to study postnatal neurogenesis in the subventricular zone (SVZ) and rostral migratory stream (RMS) of Wistar rats of both sexes. Newborn postnatal day 7 (P7) and young adult rats (P28) were exposed to pulsed electromagnetic fields (EMF) at a frequency of 2.45GHz and mean power density of 2.8mW/cm(2) for 2h. Post-irradiation changes were studied using immunohistochemical localization of Fos and NADPH-d. We found that short-duration exposure induces increased Fos immunoreactivity selectively in cells of the SVZ of P7 and P28 rats. There were no Fos positive cel... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4107172 Wed, 13 Oct 2010 23:00:00 +0100 4107172 http://www.medworm.com/index.php?rid=4107171&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20951411%26dopt%3DAbstract This study provides the first evidence of three morphological types of OSNs within the olfactory epithelium of cartilaginous fish that are similar to those found in the teleosts. In fishes there is evidence that OSNs differ functionally, including their differential olfactory bulb projections and molecular properties. The Substance P positive olfactory neurons in S. canicula may have a separate bulbar projection site that is not known, but may indicate a characteristic found in olfactory neuron subtypes in both lampreys and teleost fish. Numerous Substance P immunopositive nerves are found at the base of and in the olfactory epithelium. Some of them were observed to extend outwards almost reaching the epithelial surface. Their presumptive origin from the trigeminal nerve and their interrel... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4107171 Wed, 13 Oct 2010 23:00:00 +0100 4107171 http://www.medworm.com/index.php?rid=4107173&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20950842%26dopt%3DAbstract Authors: Simons PJ, Kummer JA, Luiken JJ, Boon L CD36 is the receptor for long chain fatty acids (LCFA), and is expressed in lingual taste cells from rodents. In these animals, CD36 has been proposed to play an important role in oral detection of LCFA, and subsequently, determines their dietary fat preference. Humans also seem to detect LCFA in the oral cavity, however, information on the molecular mechanism of this human orosensory LCFA recognition is currently lacking. The aim of our study was to investigate whether CD36 is also expressed in lingual human and porcine taste buds cells. Using fluorescence immunohistochemistry, apical CD36 expression was revealed in human and porcine taste bud cells from circumvallate and foliate papillae. These data suggest CD36 as the putative orosens... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4107173 Tue, 12 Oct 2010 23:00:00 +0100 4107173 http://www.medworm.com/index.php?rid=4075317&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20947139%26dopt%3DAbstract Authors: Kang KY, Kim JN, Chang IY, Park SH, Yoon SP Carbon tetrachloride (CCl(4)) is a potent hepatotoxic and nephrotoxic chemical. Little, however, is known about the association of CCl(4)-induced nephrotoxicity and calretinin. We hypothesized that calretinin might be localized in the proximal tubule cells and play a role against CCl(4)-induced nephrotoxicity, since the target of CCl(4) is the brush border-bearing tubule cells. CCl(4) (1ml/kg) was administrated by oral gavage to 8-week old male Sprague-Dawley rats once a week for 4 weeks. A significant increase in serum blood urea nitrogen and creatinine was confirmed by serum analysis. Calretinin immunolocalization was compared with the calbindin D-28k immunoreactivity in normal and CCl(4)-treated kidneys. Calretinin was clearly imm... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4075317 Mon, 11 Oct 2010 23:00:00 +0100 4075317 http://www.medworm.com/index.php?rid=4075318&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20943257%26dopt%3DAbstract Authors: Petricevic J, Forempoher G, Ostojic L, Mardesic-Brakus S, Andjelinovic S, Vukojevic K, Saraga-Babic M The spatial and temporal pattern of appearance of nestin, epithelial membrane antigen (EMA) and mesothelin proteins was immunohistochemically determined in the cells of normal developing and adult human meninges and meningiomas. Human meninges developed as two mesenchymal condensations in the head region. The simple squamous epithelium on the surface of leptomeninges developed during mesenchymal to epithelial transformation. Nestin appeared for the first time in week 7, EMA in week 8, while mesothelin appeared in week 22 of development. In the late fetal period and after birth, nestin expression decreased, whereas expression of EMA and mesothelin increased. EMA appeared in all... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4075318 Sun, 10 Oct 2010 23:00:00 +0100 4075318 http://www.medworm.com/index.php?rid=4061210&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20932555%26dopt%3DAbstract Acta Histochem. 2010 Oct 5; Authors: Huang S, Xie J, Zhang H PMID: 20932555 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4061210 Mon, 04 Oct 2010 23:00:00 +0100 4061210 http://www.medworm.com/index.php?rid=4061212&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20926121%26dopt%3DAbstract Authors: Tang BL, Lim YS PMID: 20926121 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4061212 Sun, 03 Oct 2010 23:00:00 +0100 4061212 http://www.medworm.com/index.php?rid=4061211&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20926122%26dopt%3DAbstract Authors: Zhang XJ, Zhou LH, Ban X, Liu DX, Jiang W, Liu XM Mammals spontaneously prefer lipid rich foods. Overconsumption of high-fat diet leads to obesity and related diseases. Recent findings indicate that taste may participate in the orosensory perception of dietary lipids and the fatty taste may contribute to a preference for and excessive consumption of dietary fat. CD36, a trans-membrane glycoprotein, which is located in the taste buds of circumvallate papillae of rodents, appears to be a plausible receptor for this fatty taste. Obese subjects present a stronger preference for fatty foods, though the mechanisms involved are complex and are not fully investigated. Our data from immunofluorescence and real-time RT-PCR showed that the expression levels of CD36 in circumvallate taste... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=4061211 Sun, 03 Oct 2010 23:00:00 +0100 4061211 http://www.medworm.com/index.php?rid=3987879&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20850173%26dopt%3DAbstract Authors: Raucci F, Di Fiore MM We performed an immunohistochemical study to determine the immunolocalization of c-kit and stem cell factor (SCF) in ovarian follicles during the reproductive cycle of the lizard, Podarcis s. sicula. Follicles were serially cut and used for histological and histochemical characterization and also for immunohistochemical detection of both c-kit and SCF. C-kit and SCF were localized in the follicles with a differing pattern with regard to the stage of sexual cycle or the cell type forming the follicular epithelium (granulosa). In pre-reproductive follicles, where the granulosa consists of three main different cytotypes, the c-kit receptor was prevalently localized on the plasmalemma of small cells, although some pyriform and intermediate cells also appeared... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3987879 Tue, 14 Sep 2010 23:00:00 +0100 3987879 http://www.medworm.com/index.php?rid=3981418&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20846710%26dopt%3DAbstract Authors: Károly N, Mihály A, Dobó E Pilocarpine-induced epileptic state (Status epilepticus) generates an aberrant sprouting of hippocampal mossy fibers, which alter the intrahippocampal circuits. The mechanisms of the synaptic plasticity remain to be determined. In our studies in mice and rats, pilocarpine-induced seizures were done in order to gain information on the process of synaptogenesis. After a 2-month survival period, changes in the levels of synaptic markers (GAP-43 and Syn-I) were examined in the hippocampus by means of semi-quantitative immunohistochemistry. Mossy fiber sprouting (MFS) was examined in each brain using Timm's sulphide-silver method. Despite the marked behavioral manifestations caused by pilocarpine treatment, only 40% of the rats and 56% of the mic... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3981418 Mon, 13 Sep 2010 23:00:00 +0100 3981418 http://www.medworm.com/index.php?rid=3962056&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20828800%26dopt%3DAbstract Authors: Acosta M, Mohamed F Numerous reports have shown the effect of photoperiod and melatonin administration on the different hormone secreting cell types in the pituitary pars distalis. The viscacha (Lagostomus maximus maximus) is a rodent with photoperiod-dependent seasonal reproduction. The aim of this study was to examine the effect of photoperiod seasonal variations and melatonin administration on the folliculostellate cells in pituitary pars distalis of viscacha. Immunohistochemistry and image analysis were used to measure the percentage of S-100-positive area (total, cellular and colloidal) and the number of folliculostellate cells. The S-100 protein was immunolocalized at intracellular (folliculostellate cells) and extracellular (follicular colloid) levels. The morphometric ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3962056 Tue, 07 Sep 2010 23:00:00 +0100 3962056 http://www.medworm.com/index.php?rid=3954716&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20817268%26dopt%3DAbstract Authors: Du J, Fan Z, Ma X, Gao Y, Wu Y, Liu S, Shen Y, Fan M, Wang S Dipeptidyl-peptidase-like protein 6 (DPP6), a member of the dipeptidyl aminopeptidase family, plays distinct roles in brain development, but its expression in embryonic craniofacial development is unknown. The expression pattern of Dpp6 in the maxillofacial region during mouse embryonic craniofacial development was analyzed by whole-mount in situ hybridization on sections and by real-time PCR analysis. Dpp6 expression was detected during mouse embryonic craniofacial development in embryos 11-13.5 days post-coitum (dpc). Real-time PCR showed high Dpp6 expression present in 11.5-13.5dpc, and this then decreased as development of maxillofacial region progressed. The expression pattern of Dpp6 suggests that Dpp6 may be i... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3954716 Wed, 01 Sep 2010 23:00:00 +0100 3954716 http://www.medworm.com/index.php?rid=3912021&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20728921%26dopt%3DAbstract Authors: Stano J, Siekel P, Neubert K, Mičieta K A synthetic substrate replacing lactose has facilitated application of a simple, rapid and sensitive method for the identification and determination of extracellular and intracellular gherkin lactase. The intracellular enzyme activity was estimated from the cell suspension, while the extracellular enzyme activity was established within the cell free cultivation medium. A suspension of gherkin cells was permeabilized by Tween 20, or Tween 80, or hexadecyltrimethyl ammonium bromide, or hexadecylpyridinium chloride or ethanol added one at a time and then immobilized by glutaraldehyde. The highest lactase activity was at pH 4.8 at a temperature of 55 degrees C. The hydrolysis of substrate was linear for 4.5h and reached 60% conversion. Th... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3912021 Thu, 19 Aug 2010 23:00:00 +0100 3912021 http://www.medworm.com/index.php?rid=3912022&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20727574%26dopt%3DAbstract Authors: Dresch RR, Zanetti GD, Kanan JH, Mothes B, Lerner CB, Trindade VM, Henriques AT, Vozári-Hampe MM The N-acetyl amino-carbohydrate specific lectin (ACL-I) was previously identified and purified by us from the marine sponge Axinella corrugata (phylum Porifera, class Demospongiae). The distribution of the specific lectin within the tissue of the sponge was studied by bright-field optical microscopy immunohistochemistry in order to better understand its physiological role in the sponge. Polyclonal antibodies were raised against purified ACL-I in mice and tested by Western blot technique. The immunohistochemical analysis of ACL-I in cross sections of A. corrugata showed that this lectin is found inside the denominated spherulous cells, which contain vesicles that store the lectin... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3912022 Tue, 17 Aug 2010 23:00:00 +0100 3912022 http://www.medworm.com/index.php?rid=3869458&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20696468%26dopt%3DAbstract Authors: Musumeci G, Loreto C, Clementi G, Fiore CE, Martinez G Osteopenia is a significant problem associated with Diabetes mellitus. Osteopenia may result in an increased delay in healing of bone fractures and subsequently affect the quality of life. We evaluated the immunohistochemical localization of TRAIL and its receptor DR5 in the femoral bone of 10-week-old Sprague-Dawley male rats treated with sesame oil (control, group 1), streptozotocin (STZ), a diabetes inducer (group 2), l-NAME, a general inhibitor of NOS activity (group 3), l-arginine (group 4), (arginine acts as a NO substrate) and iNOS immunostaining in group 1 and group 4. Histological and histochemical findings showed decreased growth of metaphyseal cartilage (which was thinner), decreased osteoid surface, and reduced... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3869458 Fri, 06 Aug 2010 23:00:00 +0100 3869458 http://www.medworm.com/index.php?rid=3854164&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20688360%26dopt%3DAbstract In this study, the immunolocalization of vascular endothelial growth factor (VEGF), VEGF receptor-1/fms-like tyrosine kinase-1 (VEGFR-1/Flt-1), VEGF receptor-2/fetal liver kinase-1 (VEGFR-2/Flk-1), proliferating cell nuclear antigen (PCNA), and CD34 was investigated in 28 cases of JNA and 20 cases of orbital cavernous hemangiomas (OCH). The immunostaining levels of VEGF, Flt-1, and Flk-1 were higher and more frequent in vascular endothelial cells of JNA than those of OCH (p<0.05). The average microvessel density (MVD) marked by CD34 in JNA was (49.3+/-9.1)/HPF (high power field), which was higher than OCH (29.1+/-6.7)/HPF (p<0.05). Immunoreactivity of PCNA was localized in both endothelial and stromal cell components of JNA, but was predominantly seen in the stromal cells. However, n... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3854164 Mon, 02 Aug 2010 23:00:00 +0100 3854164 http://www.medworm.com/index.php?rid=3823658&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20674960%26dopt%3DAbstract In this study we show that in the presence of the three-layered silicate clay montmorillonite, diluted solutions of thionine and toluidine blue develop an immediate metachromatic shift (from blue to reddish violet). Likewise, hypoemission and red shift in the fluorescence spectra of acridine orange, pyronine Y and ethidium bromide appear when montmorillonite is added to the dye solution. Cationic dyes could insert as stacked structures into the negatively charged interlamellar spaces of the clay. These spectral results indicate that on account of its strong aggregating capacity, montmorillonite is a suitable model substrate to study metachromatic reactions. PMID: 20674960 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3823658 Thu, 29 Jul 2010 23:00:00 +0100 3823658 http://www.medworm.com/index.php?rid=3791619&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20656334%26dopt%3DAbstract In this study we evaluated immunohistochemically the glial and dopaminergic systems in adult male Wistar rats. 0.5% (v/v) lead acetate in drinking water was administrated chronically over a 3-month period. Hypertrophic immunoreactive astrocytes were observed in the frontal cortex and other brain structures of the treated animals. Analysis of the astroglial features showed increased number of astrocyte cell bodies and processes in treated rats, an increase confirmed by Western blot. Particular distribution of glial fibrillary acidic protein immunoreactivity was observed within the blood vessel walls in which dense immunoreactive glial processes emanate from astrocytes. Glial changes in the frontal cortex were concomitant with reduced tyrosine hydroxylase immunoreactive neuronal processes, w... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3791619 Thu, 22 Jul 2010 23:00:00 +0100 3791619 http://www.medworm.com/index.php?rid=3791618&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20656335%26dopt%3DAbstract This study investigated the localization of AGEs in degenerated cartilage using newly identified epitope-specific antibodies to determine the linkage between the distribution of AGEs and the development and progression of OA. Osteochondral specimens of the tibial plateau from OA patients were immunostained by specific antibodies against N(varepsilon)-(carboxymethyl)lysine (CML), N(varepsilon)-(carboxyethyl)lysine (CEL), pentosidine, GA-pyridine, and RAGE. The immunohistochemical distribution of these epitopes was evaluated during cartilage degeneration. The immunoreactivity (IR) of AGEs and RAGE was stronger in cells rather than in the extracellular matrix. Higher IR of cellular CML and CEL was observed in both mild and severe OA cartilage in comparison to macroscopically intact cartilage.... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3791618 Thu, 22 Jul 2010 23:00:00 +0100 3791618 http://www.medworm.com/index.php?rid=3791617&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20656336%26dopt%3DAbstract In this study, we investigated the immunolocalization of Wnt5a protein in pelages of the dorsal skin and whisker follicles of mice. We found that in the anagen phase, dermal papilla cells showed the highest staining levels of Wnt5a protein, while in the catagen and the telogen phases the staining levels were lower. During the growth stage, Wnt5a protein was prominently located in the matrix and precortex cells in addition to the inner root sheath, outer root sheath and the dermal papilla. As the hair cycle progresses, the immunostaining of Wnt5a was gradually decreased in the catagen phase and was located in the bulge and secondary hair germ in the telogen phase. This Wnt5a immunostaining profile was consistent between dorsal skin pelages and whisker follicles. Furthermore, in an in vitro ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3791617 Thu, 22 Jul 2010 23:00:00 +0100 3791617 http://www.medworm.com/index.php?rid=3791616&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20656337%26dopt%3DAbstract Authors: Huang S, Yang L, An Y, Ma X, Zhang C, Xie G, Chen ZY, Xie J, Zhang H Abnormal hyperactivation of the hedgehog (Hh) pathway has been reported in many types of human cancers, including lung cancer. However, most reports are based on studies of fewer than three Hh target genes and the data vary between different studies. In the present report, we have determined the expression levels of several important components of the Hh pathway in lung cancers by using RT-PCR, in situ hybridization and immunohistochemistry. These molecules include Smoothened (SMO), Rab23, the downstream target platelet-derived growth factor receptor alpha (PDGFRalpha), hedgehog interacting protein (HIP) and hepatocyte nuclear factor 3-beta (HNF3beta). Our data show that some components of the hedgehog pathwa... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3791616 Thu, 22 Jul 2010 23:00:00 +0100 3791616 http://www.medworm.com/index.php?rid=3791615&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20656338%26dopt%3DAbstract This study reveals that the conus arteriosus of the longnose gar is primarily myocardial in nature, but additionally, large numbers of smooth muscle cells are present in the subendocardium. A well-developed system of adrenergic, cholinergic, substance P (SP) and neuronal nitric oxide synthase (nNOS) positive nerve terminals are found in the wall of the conus arteriosus. Coronary blood vessels running in the adventitia receive a rich supply of nNOS positive nerve fibers, thus suggesting their importance in the nitrergic control of blood flow in the conus arteriosus. The present data show that the patterns of autonomic innervation of the garfish conus arteriosus are more complex than previously appreciated. PMID: 20656338 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3791615 Thu, 22 Jul 2010 23:00:00 +0100 3791615 http://www.medworm.com/index.php?rid=3791614&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20656339%26dopt%3DAbstract Authors: Majewska M, Panasiewicz G, Szafranska B Pregnancy-associated glycoproteins (PAGs) are abundant embryo-originated products expressed in the pre-placental trophoblast and later in the post-implantational chorionic epithelium of some ungulate species. This paper describes the cellular immunolocalization of the chorionic PAG family in the epitheliochorial placenta type of the alpaca (Lama pacos-Lp), in which the PAGs were named 'LpPAGs'. Placental Lp sections (5mum) of different females near mid-pregnancy (150 days post coitum; dpc), advanced pregnancy (244-263 dpc) and late pregnancy (347 dpc) were used for cross-species (heterologous-ht) double fluorescent immunohistochemistry (htdF-IHC). The htdF-IHC was performed with primary rabbit polyvalent anti-porcine PAG polyclonals. The... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3791614 Thu, 22 Jul 2010 23:00:00 +0100 3791614 http://www.medworm.com/index.php?rid=3791613&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20656340%26dopt%3DAbstract Authors: De Jong PT, Tigchelaar W, Van Noorden CJ, Van der Vis HM In vitro and in vivo studies have suggested that polyethylene wear particles are the main cause for osteolysis in prosthetic loosening. Elevated amounts of proteases including gelatinases (or matrix metalloproteinases MMP-2 and MMP-9) have been found in fibrous tissue interfaces of loosened total hip arthroplasties suggesting that proteolysis plays a role in osteolysis. The presence of proteases does not mean that they are active, because activity of proteases is highly regulated at the post-translational level. We investigated whether the activity of two major proteases that are active extracellularly and have been associated with loosening, MMP-2 and MMP-9, is involved in loosening of non-cemented hip implants with pol... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3791613 Thu, 22 Jul 2010 23:00:00 +0100 3791613 http://www.medworm.com/index.php?rid=3758979&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20619444%26dopt%3DAbstract In conclusion, changes in skeletal muscles occur in this mouse model of heart failure and these changes were antagonized by losartan. In contrast to heart failure patients, in the mouse model a shift to the oxidative phenotype of skeletal muscle was noted, possibly due to enhanced calcineurin expression. PMID: 20619444 [PubMed - as supplied by publisher] (Source: Acta Histochemica) Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3758979 Tue, 06 Jul 2010 23:00:00 +0100 3758979 http://www.medworm.com/index.php?rid=3733606&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20599250%26dopt%3DAbstract Authors: Vitanova LA The NMDA receptors are ionotropic glutamate receptors that are involved in a variety of functions in the nervous system and in particular in the retina. They are composed of NR1 and NR2 subunits. The NMDA receptors have been fairly well studied in the retina of mammals, however, there is only limited information concerning these receptors in the retinas of lower vertebrates. The aim of the present study was to investigate immunocytochemically the NR1, NR2A and NR2B subunits of the NMDA receptors in the frog retina. Six primary antibodies were used. Three of them were directed to different splice variants of the NR1 subunit and the remaining three variants directed to NR2 subunits. All antibodies showed well expressed labeling in the frog retina. The labels had a pu... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3733606 Thu, 01 Jul 2010 23:00:00 +0100 3733606 http://www.medworm.com/index.php?rid=3733624&cid=s_31704_60_f&fid=31704&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20598349%26dopt%3DAbstract Authors: Karray-Chouayekh S, Baccouche S, Khabir A, Sellami-Boudawara T, Daoud J, Frikha M, Jlidi R, Gargouri A, Mokdad-Gargouri R Infiltrating ductal carcinoma (IDC) of the breast is a result of genetic alterations that affect the regulation of the cell cycle check-point and apoptosis. The aim of the present study was analysis using immunohistochemical localization of mouse double minute-2 (mdm2), p16INK4a, p53, bax and bcl-2 markers in Tunisian patients with breast IDC and to determine if there was correlation with the major clinico-pathological parameters and with survival of patients. We showed that the expression of p53, p16INK4a, mdm2, bcl-2, and bax was observed in 46.3%, 20.7%, 38%, 50% and 11.9% of cases, respectively. Statistical analysis revealed that positive expression of ... Acta Histochemica journals http://www.medworm.com/rss/comments.php?id=3733624 Tue, 29 Jun 2010 23:00:00 +0100 3733624