MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Acta Microbiologica et Immunologica Hungarica' source. Fri, 27 Jan 2012 23:58:37 +0100 http://www.medworm.com/index.php?rid=5560004&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207283%26dopt%3DAbstract Authors: Naganandini S, Balachandar D, Kumar K Abstract This investigation was carried out based on the hypothesis that there may be some pseudomonad strains, which could exist in rhizosphere of plant species contributing multifaceted beneficial activities. For this purpose, 21 pseudomonad isolates from the rhizosphere of rice, cultivated in western parts of Tamil Nadu were screened. All the 21 isolates were authenticated as pseudomonads by a genus-specific PCR screening. The molecular diversity of these isolates was investigated by Amplified Ribosomal DNA Restriction Analysis (ARDRA) and the dendrogram obtained from the analysis revealed that all the 21 isolates clustered into seven groups. Further, these isolates were screened for plant growth promoting activities such as diazotr... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5560004 Thu, 01 Dec 2011 05:00:00 +0100 5560004 http://www.medworm.com/index.php?rid=5560003&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207284%26dopt%3DAbstract Authors: Bagherpour G, Fooladi AA, Mehrabadi JF, Nourani MR, Einollahi B Abstract Uropathogenic Escherichia coli (UPEC) bacteria are the principal cause of urinary tract infections (UTI). Because these bacteria propagate intracellularly, the cellular immune response is an important factor in UTIs. Therefore, we designed a genetic construct to induce a cellular immune response. In order to develop a genetic construct that induces strong cellular immunity against this pathogen, we used the fimH synthetic gene according to mammalian codon usage, and the gene expression was compared with wild type codon usage. Initially, we designed two constructs, pVAX/fimH mam and pVAX/fimH wt, which contain mammalian and wild type codon usage, respectively. The Cos-7 cell line was transfected separa... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5560003 Thu, 01 Dec 2011 05:00:00 +0100 5560003 http://www.medworm.com/index.php?rid=5560002&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207285%26dopt%3DAbstract In conclusion, despite the advances in antibiotic therapy and vaccine development, bacterial meningitis still is a health problem. S. pneumoniae, H. influenzae, and N. meningitidis are the main sources of bacterial meningitis, but other organisms such as E. coli should also be suspected, when a case is admitted to a referral pediatric hospital. PMID: 22207285 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5560002 Thu, 01 Dec 2011 05:00:00 +0100 5560002 http://www.medworm.com/index.php?rid=5560001&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207286%26dopt%3DAbstract Authors: Kaya EG, Ozbilge H, Ustundag MB, Torun YA Abstract The aim of this study was to investigate the effects on the immune response of levamisole alone and in conjunction with Candida albicans stimulation in human macrophage cell culture by determining the alterations in the levels of cytokine release.Levamisole treatment was performed before, during and after infecting U-937 human macrophage cells with C. albicans. In cell supernatants, interleukin (IL)-1b, IL-12, IL-18, tumour necrosis factor alpha (TNF-α) levels were measured by ELISA.In vitro levamisole treatment accompanied by C. albicans stimulation significantly increased IL-12, IL-1β and IL-18 production in macrophage cells (p < 0.05). It was observed that when administered before C. albicans infection, levamisole ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5560001 Thu, 01 Dec 2011 05:00:00 +0100 5560001 http://www.medworm.com/index.php?rid=5560000&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207287%26dopt%3DAbstract Authors: Kahbazi M, Fahmizad A, Armin S, Ghanaee RM, Fallah F, Shiva F, Golnabi A, Jahromy MH, Arjomandzadegan M, Karimi A Abstract Viruses are frequent causes of upper respiratory tract infections in children. We investigated the viral aetiology of community-acquired upper respiratory tract infections (URIs) in young children treated as outpatients in community settings.During November 2008, nasal swab specimens were taken from children with recent onset of upper respiratory tract infections. The patients attended day care or primary schools; the specimens were randomly obtained by pediatricians from schools and childcare institutions and sent for identification by PCR method.A total of 300 specimens were collected. From all samples, 40.67% were positive for at least 1 virus, viz.... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5560000 Thu, 01 Dec 2011 05:00:00 +0100 5560000 http://www.medworm.com/index.php?rid=5559999&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207288%26dopt%3DAbstract Authors: Mamishi S, Ahmadi F, Ahmadi M, Rezaei N Abstract Chronic granulomatous disease (CGD) is a rare primary immunodeficiency disease, affecting phagocytic blood cells, which predispose patients to recurrent infectious complications. Herein, an 11-year-old girl is described who presented with liver abscess at the age of 9 years. Positive dihydrorhodamine (DHR) and nitrobluetetrazolium (NBT) tests confirmed the diagnosis of CGD for the patient. Anti-tuberculosis drugs and parenteral antibiotic therapy were started. Unusual visceral abscess and recurrent infections should be considered as an alarm for primary immunodeficiency diseases, while early diagnosis and appropriate treatment could prevent severe complications and even death in this group of patients. PMID: 22207288 [Pu... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5559999 Thu, 01 Dec 2011 05:00:00 +0100 5559999 http://www.medworm.com/index.php?rid=5559998&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207289%26dopt%3DAbstract In this study, we performed an oligonucleotide microarray screening of 780 inflammation-associated genes in HCMV-infected aortic SMC (AoSMC). The expression of 31 genes was stimulated and 24 genes were down-regulated following infection with HCMV strain DC-134. Following infection with HCMV strain AD-169 infection, we found 24 genes to be stimulated and 32 genes to be down-regulated. Among these were primarily genes encoding for CC and CXC chemokines, adhesion molecules, and tumor necrosis factor (TNF) receptor superfamily members, apoptosis-related factors, signal transduction molecules and transcription regulators. The up-regulated genes included matrix metalloproteinase (MMP)-1 and MMP-3 in HCMV infected cells. Using RT-PCR and enzyme immunoassay we found stimulated expression of MMP-1 ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5559998 Thu, 01 Dec 2011 05:00:00 +0100 5559998 http://www.medworm.com/index.php?rid=5559997&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207290%26dopt%3DAbstract In conclusion, genotype and even subtype distribution may be important in association with diseases, therefore using this classification for characterization of intraspecies diversity of TTV1 is proposed. PMID: 22207290 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5559997 Thu, 01 Dec 2011 05:00:00 +0100 5559997 http://www.medworm.com/index.php?rid=5559996&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207291%26dopt%3DAbstract Authors: Gupta R, Kalpana Abstract In the present study, the optimization of production and reaction conditions of polygalacturonase produced by a fungus Byssochlamys fulva MTCC 505 was achieved. The production of polygalacturonase with a considerable activity of 1.28 IU/ml was found when the culture was shaken at 30°C for 5 days in 100 ml of medium containing (w/v) 10 g/l pectin, 2 g/l NaNO3, 1 g/l KH2PO4, 0.5 g/l KCl, 0.5 g/l MgSO4. 7H2O, 0.001 g/l FeSO4. 7H2O, 0.001 g/l CaCl2. The best carbon and nitrogen source for this enzyme were pectin (1%) and Ca(NO3)2 (0.1%), respectively. The enzyme gave maximum activity at incubation time of 72 h, temperature of 30°C and pH 4.5. During the optimization of reaction conditions, the enzyme showed maximum activity in sodium citrate buffer... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5559996 Thu, 01 Dec 2011 05:00:00 +0100 5559996 http://www.medworm.com/index.php?rid=5559995&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207292%26dopt%3DAbstract Authors: Vilibic-Cavlek T, Ljubin-Sternak S, Kos L, Mlinaric-Galinovic G Abstract There is a high degree of variability in the serologic response to Epstein-Barr virus (EBV) infection, especially in viral capsid antigen (VCA)-IgM antibodies. Therefore, additional tests are needed to confirm primary infection.We evaluated the value of IgG avidity determination in diagnosis of EBV infection in immunocompetent and immunocompromised patients.A total of 236 serum samples from immunocompetent patients with symptoms suggestive of EBV infection were tested for the presence of VCA-IgM/IgG antibodies and IgG avidity. Using IgG avidity, acute primary infection was confirmed in 56.7% of the immunocompetent patients with positive and in 1.8% of patients with negative VCA-IgM. Recent primary inf... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5559995 Thu, 01 Dec 2011 05:00:00 +0100 5559995 http://www.medworm.com/index.php?rid=5559994&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207293%26dopt%3DAbstract Authors: Asadollahi K, Taherikalani M, Maleki A, Alizadeh E, Valadbaigi H, Soroush S, Maleki H, Asadollahi P, Emaneini M Abstract The aim of the present study was to investigate, for the first time, the diversity of the genes encoding aminoglycoside-modifying enzymes (AME) and their association with class 1 integrons in Iranian Acinetobacter baumannii strains.A total of 100 multidrug resistant A. baumannii, isolated from eight distinct hospitals in Tehran, were enrolled in this study. Susceptibility of these isolates to antimicrobial agents including gentamicin and amikacin was determined by E-test. Aminoglycoside resistant isolates were then tested by PCR for AME genes, including aphA6, aacC1, aacC2, aacA4, aadB, aadA1, classes 1 integron, 5'-CS-3' and typed by RAPD PCR.The rate o... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5559994 Thu, 01 Dec 2011 05:00:00 +0100 5559994 http://www.medworm.com/index.php?rid=5559993&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207294%26dopt%3DAbstract Authors: Bohus V, Kéki Z, Márialigeti K, Baranyi K, Patek G, Schunk J, Tóth EM Abstract Ultrapure waters (UPWs) containing low levels of organic and inorganic compounds provide extreme environment. On contrary to that microbes occur in such waters and form biofilms on surfaces, thus may induce corrosion processes in many industrial applications. In our study, refined saltless water (UPW) produced for the boiler of a Hungarian power plant was examined before and after storage (sampling the inlet [TKE] and outlet [TKU] waters of a storage tank) with cultivation and culture independent methods. Our results showed increased CFU and direct cell counts after the storage. Cultivation results showed the dominance of aerobic, chemoorganotrophic α-Proteobacteria in both samples. In case ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5559993 Thu, 01 Dec 2011 05:00:00 +0100 5559993 http://www.medworm.com/index.php?rid=5312244&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21983319%26dopt%3DAbstract This study revealed an additional aspect of the toxicological mechanisms of the pesticides through which they suppress the plant growth. PMID: 21983319 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5312244 Thu, 01 Sep 2011 04:00:00 +0100 5312244 http://www.medworm.com/index.php?rid=5312243&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21983320%26dopt%3DAbstract Authors: Azmi W, Thakur M, Kumar A Abstract The cheese whey, a by-product of dairy industry proved to be an attractive substrate for production of β-carotene. The β-carotene production from Mucor azygosporus MTCC 414 by using deproteinized waste whey filtrate under submerged fermentation was investigated. Various fermentation variables, such as lactose content in whey, initial pH, production temperature, incubation time, and carbon and nitrogen sources played significant role on β-carotene production. Maximum β-carotene production (385 μg/g dcw) was obtained with the whey (pH 5.5) containing 3.5% (w/v) lactose supplemented with soluble starch at (1.0%, w/v) at 30°C after a 5 days incubation. Moreover, unlike other microorganisms which utilize pre-hydrolyzed lactose, this Muco... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5312243 Thu, 01 Sep 2011 04:00:00 +0100 5312243 http://www.medworm.com/index.php?rid=5312242&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21983321%26dopt%3DAbstract Authors: Baintner K, Kocsis B, Kovács K, Péterfi Z, Kökény G, Hamar P Abstract Binding of fluorescein isothiocyanate-labeled concanavalin A to a series of molecular species of lipopolysaccharide (LPS), purified from pathogenic bacteria, was studied via agarose gel precipitation experiments and the results were compared with available structural data.The LPS species could be divided into ConA-reactive and non-reactive ones. Reactivity resided in the O-specific chain of LPS, and binding to the lipid A or core moieties of LPS could not be demonstrated by the present methods. The α-D-glucose or α-D-mannose residues of the repeating O-specific oligosaccharide units appeared to be recognized by ConA, except when blocked by steric hindrance. Specificity of the reaction was verified ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5312242 Thu, 01 Sep 2011 04:00:00 +0100 5312242 http://www.medworm.com/index.php?rid=5312241&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21983322%26dopt%3DAbstract Authors: Darban-Sarokhalil D, Fooladi AA, Bameri Z, Nasiri MJ, Feizabadi MM Abstract Cytochrome P450 CYP141 is an intermediary metabolic and respiratory protein that interferes with oxidation reduction in Mycobacterium tuberculosis. This conserved protein has also been debated as a hypothetical target for therapeutics. We used the sequences of CYP141 gene to develop a PCR for rapid detection of Mycobacterium tuberculosis from respiratory specimens. The sensitivity of this PCR for culture positive-smear positive and culture positive-smear negative samples were 92% and 62.5%, respectively. The overall sensitivity and specificity of this PCR was 85.7% and 97.8%. As compared with other studies, it appears that the CYP141 gene is a good target for direct detection of M. tuberculosis fro... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5312241 Thu, 01 Sep 2011 04:00:00 +0100 5312241 http://www.medworm.com/index.php?rid=5312240&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21983323%26dopt%3DAbstract Authors: Aligholi M, Emaneini M, Taherikalani M, Shahsavan S, Jabalameli F, Asadollahi P, Khoramian B, Eslampour MA Abstract The synergy between gentamicin and vancomycin, teicoplanin, ampicillin and linezolid was studied by time-kill method. Two clinical vancomycin resistant enterococci (VRE) and two vancomycin susceptible enterococci (VSE) isolates were used. Different concentrations of antibiotics were combined. Two VSE strains and the control strain exhibited synergism with the combination of gentamicin, vancomycin, teicoplanin, ampicillin and linezolid. Two VRE strains exhibited synergism with the combination of gentamicin and ampicillin. Synergy between gentamicin and vancomycin, teicoplanin and linezolid was not observed against these isolates. The VRE isolates were positive... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5312240 Thu, 01 Sep 2011 04:00:00 +0100 5312240 http://www.medworm.com/index.php?rid=5312239&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21983324%26dopt%3DAbstract In this study we investigated the frequency of MRSA colonization and their antibiotic susceptibility patterns in 1-6 years old children of day-care centers in Hamadan, West of Iran.Five hundred nasal swabs were collected from children of 27 day-care centers that had no risk factors for colonization by S. aureus. The specimens were cultured for isolation of S. aureus by standard methods. Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. For evaluation of the frequency of erythromycin induced clindamycin resistance, disk approximation test (D-test) was applied.Totally, 148 (29.6%) children were colonized by S. aureus. Out of 260 male, 94 (36.2%) and of 240 female, 54 (22.5%) cases were nasal carriers of S. aureu... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5312239 Thu, 01 Sep 2011 04:00:00 +0100 5312239 http://www.medworm.com/index.php?rid=5312238&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21983325%26dopt%3DAbstract Authors: Gul M, Dogan E, Kirecci E, Ucmak H, Dirican E, Karadag A Abstract Serratia ficaria was first described in 1979 as a Gram-negative facultative anaerobic rod. S. ficaria was found in figs, but also isolated from human specimens in a few cases. We now report an isolate of S. ficaria from sputum specimen.A 46-year-old man was suffering from a chronic renal failure of five years, four months of peritoneal dialysis and one week of fever due to respiratory tract infection, accompanied by cough. Sputum culture yielded a Gram-negative rod. It was identified as S. ficaria and the antibiotic susceptibility test was performed by automated Vitek II (bioMerieux). The tested S. ficaria strain was susceptible to amikacin, gentamicin, cefepime, trimethoprim-sulfamethoxazole, imipenem, mero... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5312238 Thu, 01 Sep 2011 04:00:00 +0100 5312238 http://www.medworm.com/index.php?rid=5312237&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21983326%26dopt%3DAbstract In this study we compared the nasal carriage of healthy children attending DCCs in Szeged, Hungary in 2003/2004, when nobody was vaccinated, and in 2010, when already 1/5 of the children received PCV-7. Significant differences were observed in the serotype distribution, representing a marked shift from the previously widespread vaccine-types (mostly 6A or 14) to others (11A and 23F). The new serotypes showed higher antibiotic susceptibility. The bacterium exchange between children was clear from the pulsed-field gel electrophoresis (PFGE) patterns, and the circulation of certain international clones plays also a role in these dynamic changes. PMID: 21983326 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5312237 Thu, 01 Sep 2011 04:00:00 +0100 5312237 http://www.medworm.com/index.php?rid=5157221&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21778135%26dopt%3DAbstract Authors: PMID: 21778135 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5157221 Thu, 25 Aug 2011 16:24:02 +0100 5157221 http://www.medworm.com/index.php?rid=5157220&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21778136%26dopt%3DAbstract Authors: PMID: 21778136 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5157220 Thu, 25 Aug 2011 16:24:02 +0100 5157220 http://www.medworm.com/index.php?rid=5051972&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21715278%26dopt%3DAbstract Authors: Lajkó E, Pállinger E, Csaba G In a previous experiment thyrotropin (TSH) increased the triiodothyronine (T3) production of Tetrahymena and chorionic gonadotropin (HCG) moderately overlapped the effect. At present the production of three amino acid type (histamine, serotonin, epinephrine) and one peptide (endorphin) hormones were studied under the effect of TSH or HCG, in tryptone-yeast (TY) or salt (Losina-Losinsky) medium. The duration of the effect was 10 min. TSH significantly (with almost 20%) decreased epinephrine production in TY medium and HCG similarly decreased epinephrine and increased histamine level. In salt solution TSH as well as HCG decreased the level of serotonin. The results show that at this low level of phylogeny TSH effect is not completely thyroxine-spe... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5051972 Tue, 31 May 2011 23:00:00 +0100 5051972 http://www.medworm.com/index.php?rid=5051971&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21715279%26dopt%3DAbstract Authors: Sayed WF Spores of four Frankia strains, the nitrogen-fixing actinomycete, were exposed to short wavelength UV-C radiation of 254 nm at 1 lux cm2 (0.24 mw cm2 of energy) for 10 min. The used strains were HFP020203, UGL020604, UGL020602q and ORS021001. Exposure to UV was followed by reactivation with visible white light at 327.4 lux cm2 for the same period of time. Spore germination percentage, spore protein content, and cell growth were damaged by this treatment. The lower and higher percentages of reduction in spore germination were 32 and 63% and, for the same strains, the recovery by white light was 7.2 and 37%. The lower percentages of UV damage and subsequent low recovery were recorded for strain ORS021001 that is considered more resistant to UV than the other strains. Th... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5051971 Tue, 31 May 2011 23:00:00 +0100 5051971 http://www.medworm.com/index.php?rid=5051970&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21715280%26dopt%3DAbstract Authors: Gholami-Parizad E, Taherikalani M, Mozaffar-Sabet NA, Asmar M, Gholami-Parizad S, Khosravi A, Emaneini M, Asadollahi P Hepatitis B virus (HBV) transmission via blood and other body fluids from infected individuals to healthy people has been largely demonstrated. However, in the current literature, there is little information available on the potential role of cerumen in HBV transmission.Cerumen and blood were collected from 70 patients infected with HBV and 70 volunteer healthy people were selected as the control group, and the samples were evaluated by ELISA and Real-time PCR.All the patients proved positive for HBsAg and anti HBc total. Sixty-one of the 70 cerumen samples of cases (82.1%) and 5 (7%) of controls were positive for HBV DNA with ranges from 1.53 × 102 to 2.9 ×... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5051970 Tue, 31 May 2011 23:00:00 +0100 5051970 http://www.medworm.com/index.php?rid=5051969&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21715281%26dopt%3DAbstract Authors: Sahebekhtiari N, Nochi Z, Eslampour MA, Dabiri H, Bolfion M, Taherikalani M, Khoramian B, Zali MR, Emaneini M Staphylococcus aureus is considered one of the most important food borne pathogens.A total of 111 isolates of S. aureus were cultured from raw milk samples during January 2009 to June 2009 from Tehran and Mashhad. The coagulase gene polymorphism and the prevalence of classical enterotoxin genes of S. aureus strains were determined by PCR-RFLP (restriction fragment length polymorphism) and Multiplex-PCR. Disk diffusion method was used to determine the susceptibility of isolates to antimicrobial agents as instructed by Clinical and Laboratory Standards Institute.Sixty-seven % of the isolates harboured one or more enterotoxin genes. The most prevalent gene was sec, found ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5051969 Tue, 31 May 2011 23:00:00 +0100 5051969 http://www.medworm.com/index.php?rid=5051968&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21715282%26dopt%3DAbstract Authors: Faludi I, Szabó AM Chlamydophila pneumoniae is an obligate intracellular human pathogen, which causes acute respiratory tract infections and can also cause chronic infections.C. pneumoniae possess type III secretion system (TTSS), which allows them to secrete effector molecules into the inclusion membrane and the host cell cytosol. Low calcium response protein E (LcrE) is a part of TTSS. The gene of LcrE in a 6His-tagged form was cloned from C. pneumoniae CWL029, expressed and purified from Escherichia coli using the HIS-select TALON CellThru Resin, this gene was also cloned into a eukaryotic expression vector (pΔRC). One group of BALB/c mice received an intramuscular pΔRC inoculation then the mice were immunized with purified LcrE protein; the second group of mice was immu... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5051968 Tue, 31 May 2011 23:00:00 +0100 5051968 http://www.medworm.com/index.php?rid=5051967&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21715283%26dopt%3DAbstract Authors: Patyi M, Varga E, Kristóf K Methicillin-resistant Staphylococcus aureus (MRSA) is currently one of the most prevalent antibiotic-resistant pathogens in hospitals, but it is also emerging as a community-acquired pathogen. We analysed the clinical and microbiological data of the patients in a county teaching hospital regarding MRSA. During the examination period (1996-2010), four outbreaks and one pseudo-outbreak occurred. It also became evident that health care workers and their families are possibly at risk of becoming carriers of MRSA. The importance of the molecular epidemiological investigation (pulsed-field gel-electrophoresis (PFGE)) typing and hygienic measures in order to detect and control MRSA outbreaks must be emphasised. Following infection control guidelines seems... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5051967 Tue, 31 May 2011 23:00:00 +0100 5051967 http://www.medworm.com/index.php?rid=5051966&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21715284%26dopt%3DAbstract Authors: Horváth A, Neubrandt DM, Ghidán A, Nagy K Demodex mites are ectoparasites often found in follicles of facial skin. Their role in human diseases is under investigation, and a growing number of studies indicated that they contribute to chronic inflammatory conditions of the skin, such as rosacea, blepharitis, otitis externa, alopecia and folliculitis. In our study we tested 96 healthy adults for the presence of Demodex mites. Risk factors influencing presence of mites and skin types of the tested individuals were evaluated. We found Demodex folliculorum or Demodex brevis in 17.7% of the samples, more frequently in males (21.9%) and in older adults (20%). Use of make-up seems to reduce the likelihood of Demodex carriage, while pet ownership, use of shared items and living in cl... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5051966 Tue, 31 May 2011 23:00:00 +0100 5051966 http://www.medworm.com/index.php?rid=5006598&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21715285%26dopt%3DAbstract Authors: Szomor KN, Rigó Z, Bán E, Nagy L, Szalkai T, Balogh Z, Ferenczi E, Takács M The presence of WNV in Europe has been well known for decades, although the first human infections and avian outbreaks were diagnosed in Hungary only in 2003. An annual average of 6-8 cases of the neuroinvasive form of WNV infection has been detected in the region since then, but a higher number (17) of WNV associated neuroinvasive disease occurred in 2008.In 2004, a surveillance system was established for monitoring WNV-associated meningo-encephalitis cases in Hungary, but a milder type of illness (with fever, rash and/or influenza like symptoms) is not followed. Fifty-two sera of 45 patients with mild clinical symptoms (fever, exanthema) were tested for anti-WNV antibodies in 2008 in a retrospecti... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=5006598 Tue, 31 May 2011 23:00:00 +0100 5006598 http://www.medworm.com/index.php?rid=4699751&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450550%26dopt%3DAbstract Authors: Czövek P, Király I Morchella conica Pers. strains of the study were isolated from fruit bodies collected in ash-mixed forests. At first, the strains were cultured on potato dextrose agar (PDA), then on modified Murashige and Skoog (MS*) solid agar media. A normal-growing strain was chosen for the trehalase induction experiments. During the trehalase induction treatment, mycelia were grown in liquid culture containing different concentrations of trehalose. After the induction period of trehalase enzymes, physiological state of the mycelium and the oxidative stress were monitored in the vegetative mycelia by measuring the change of the malondialdehyde content, superoxide dismutase enzyme activity, the fresh and dry weight. The examined Morchella conica strain utilized the treh... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4699751 Tue, 01 Mar 2011 00:00:00 +0100 4699751 http://www.medworm.com/index.php?rid=4699750&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450551%26dopt%3DAbstract Authors: Faludi I, Szabó AM, Burián K, Endrész V, Miczák A Mycobacterium smegmatis is a species of rapidly growing saprophytes with a number of properties that make it an effective vaccine vector. Recombinant M. smegmatis expressing protective antigens of different pathogens and molecules modulating the immune responses offers some potential for reduction of the burden of tuberculosis, HIV and hepatitis B infections. This paper discusses the molecular methods used to generate recombinant M. smegmatis and the results obtained with some of these recombinants. PMID: 21450551 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4699750 Tue, 01 Mar 2011 00:00:00 +0100 4699750 http://www.medworm.com/index.php?rid=4699744&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450552%26dopt%3DAbstract Authors: Dencs A, Farkas A, Gyugos M, Kurcz A, Puskás E, Tresó B, Rusvai E, Barcsay E, Takács M A nosocomial Hepatitis B virus (HBV) outbreak at a paediatric onco-haematology unit was investigated using molecular biological methods to determine the origin of the infections. The National Reference Laboratory of Hepatitis Viruses received seven HBsAg positive sera from patients and one from the brother of a patient. A fragment of the preS1/preS2/S genes from all samples was amplified, the PCR products were sequenced and a rooted phylogenetic tree was constructed. All nucleotide sequences from the different patients were very similar and 6 of the 8 sequences were identical, suggesting a common origin of the infections. These sequences were closely related to those amplified from a noso... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4699744 Tue, 01 Mar 2011 00:00:00 +0100 4699744 http://www.medworm.com/index.php?rid=4699742&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450553%26dopt%3DAbstract Authors: Shahsavan S, Jabalameli L, Maleknejad P, Aligholi M, Imaneini H, Jabalameli F, Halimi S, Taherikalani M, Khoramian B, Eslampour MA, Feizabadi MM, Emaneini M Methicillin-resistant Staphylococcus aureus (MRSA), particularly the multidrug-resistant clones, is an increasing worldwide problem. The average incidence rate of MRSA in Tehran was found to be over 40%. A total of 140 MRSA isolates obtained from patients attending a teaching hospital in Tehran, from May 2009 to December 2009, were included in this study. The antimicrobial susceptibility profile of MRSA isolates was determined by the agar disk diffusion method. Molecular analysis of MRSA strains was accomplished by Pulsed-Field Gel Electrophoresis (PFGE) and Multi-locus sequence typing (MLST). Detection of mecA gene was us... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4699742 Tue, 01 Mar 2011 00:00:00 +0100 4699742 http://www.medworm.com/index.php?rid=4699741&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450554%26dopt%3DAbstract Authors: Pállinger E, Horváth Z, Csóka M, Kovács GT, Csaba G Histamine, serotonin and triiodothyronine (T3) content of different circulating lymphocyte subsets of leukemic (acute lymphocytic leukemia, ALL) and non-leukemic (control) children were investigated by multicolor flow cytometry. The hormone contents of the cells were followed from the time of diagnosis till the end of treatment. Each hormone could be detected in every time in the investigated cell types, although the amounts of them changed during the treatment.T lymphocytes: Significantly lower amount of serotonin was found in each T cell subsets (Th, Tc and activated T lymphocytes) of leukemic children compared to the healthy control group at the time of diagnosis and it was permanently low during the maintenance therap... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4699741 Tue, 01 Mar 2011 00:00:00 +0100 4699741 http://www.medworm.com/index.php?rid=4699738&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450555%26dopt%3DAbstract In this study a collection of 145 M. tuberculosis isolates was used to evaluate the conferring mutations in nucleotide 1388 of katG gene (KatG463) in resistance to isoniazid. A PCR-RFLP method was applied in comparison with DNA sequencing and anti-mycobacterial susceptibility testing. From all studied patients, 98 (67.6%) were men, 47 (32.4%) were women, 3% were <15 and 9% were >65 years old; male to female ratio was 1:2.4. PCR result of katG for a 620-bp amplicon was successful for all purified M. tuberculosis isolates and there was no positive M. tuberculosis culture with PCR negative results (100% specificity). Subsequent PCR RFLP of the katG identified mutation at KatG463 in 33.3%, 57.8% and 59.2% of our clinically susceptible, multidrug resistant TB (MDR) and extensively drug re... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4699738 Tue, 01 Mar 2011 00:00:00 +0100 4699738 http://www.medworm.com/index.php?rid=4699723&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450556%26dopt%3DAbstract We describe a PCR assay for fast detection of M. tuberculosis and M. bovis.As a BLAST and BLASTP search we selected regulatory gene whiB7 that encodes multi-drug resistance in this bacterium. Thirty clinical isolates of M. tuberculosis were sequenced and all the mutations in gene whiB7 were detected. The best set of several pairs of primers was selected and used in comparison by rpoB gene for differentiation of M. bovis, M. avium, M. kansasii, M. phlei, M. fortuitum, M. terrae, seven non-pathogenic Mycobacterium isolates and 30 clinical isolates of M. tuberculosis.It was proved that only clinical isolates of M. tuberculosis and M. bovis have positive bands of 667 bp whiB7. Other non-tuberculous and non-pathogenic isolates did not show any positive sign. Furthermore, 667-bp PCR products of ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4699723 Tue, 01 Mar 2011 00:00:00 +0100 4699723 http://www.medworm.com/index.php?rid=4699708&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21450557%26dopt%3DAbstract In this study, we have surveyed the nasal carriage rate of students of the Semmelweis University, by screening 300 volunteers. We have determined the antibiotic sensitivity of the isolates by Etest, and their genetic relatedness by pulsed-fieled gel electrophoresis. The nasal carriage rate of S. aureus was found to be 29.3%, and that of MRSA only 0.67% (2/300). The isolates were generally sensitive to antibiotics, except for macrolides. We could observe a noticeably great genetic diversity, even among strains deriving from students of the same university group. PMID: 21450557 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4699708 Tue, 01 Mar 2011 00:00:00 +0100 4699708 http://www.medworm.com/index.php?rid=4338941&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21183420%26dopt%3DAbstract Authors: PMID: 21183420 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4338941 Wed, 01 Dec 2010 00:00:00 +0100 4338941 http://www.medworm.com/index.php?rid=4338940&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21183421%26dopt%3DAbstract Authors: Sinkovics JG In leukemic mice, the native host's explicit and well-defined immune reactions to the leukemia virus (a strong exogenous antigen) and to leukemia cells (pretending in their native hosts to be protected "self" elements) are extinguished and replaced in GvHD (graft-versus-host disease) by those of the immunocompetent donor cells. In many cases, the GvHD-inducer donors display genetically encoded resistance to the leukemia virus. In human patients only antileukemia and anti-tumor cell immune reactions are mobilized; thus, patients are deprived of immune reactions to a strong exogenous antigen (the elusive human leukemia-sarcoma retroviruses). The innate and adaptive immune systems of mice have to sustain the immunosuppressive effects of leukemia-inducing retroviruses... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4338940 Wed, 01 Dec 2010 00:00:00 +0100 4338940 http://www.medworm.com/index.php?rid=4338939&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21183422%26dopt%3DAbstract Authors: Rajčáni J, Asványi-Molnár N, Szathmary S Lymphomas are solid tumors consisting of lymphoid cells; they form a heterogeneous group of less or more malignant disorders. A portion of lymphomas develop due to latent herpesvirus infections established in B and/or T-lymphocytes. The basis for latency is a lifelong presence of the circularized covalently linked viral genome within nuclei of carrier lymphocytes. In certain cases, however, the essential event leading to tumor formation is the integration of a portion(s) of viral DNA into the host cell DNA. This leads to rearrangements within the host cell genome on one hand, and, on other hand, to unregulated expression of oncoproteins encoded by the integrated fragment. Our review deals with mechanisms of lymphoma formation regard... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4338939 Wed, 01 Dec 2010 00:00:00 +0100 4338939 http://www.medworm.com/index.php?rid=4338938&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21183423%26dopt%3DAbstract Authors: Kaur H, Kaur A, Saini HS, Chadha BS A wild type Aspergillus terreus GD13 strain, chosen after extensive screening, was optimized for lovastatin production using statistical Box-Behnken design of experiments. The interactive effect of four process parameters, i.e. lactose and soybean meal, inoculum size (spore concentration) and age of the spore culture, on the production of lovastatin was evaluated employing response surface methodology (RSM). The model highlighted the positive effect of soybean meal concentration and inoculum level for achieving maximal level of lovastatin (1342 mg/l). The optimal fermentation conditions improved the lovastatin titre by 7.0-folds when compared to the titres obtained under unoptimized conditions. PMID: 21183423 [PubMed - in process] (Sourc... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4338938 Wed, 01 Dec 2010 00:00:00 +0100 4338938 http://www.medworm.com/index.php?rid=4338937&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21183424%26dopt%3DAbstract Authors: Csaba G, Lajkó E, Pállinger E In order to approach their natural conditions, populations of Tetrahymena were kept in Losina-Losinky's salt solution for 1 h, than in the tryptone+yeast medium. During this time they were treated with histamine, serotonin or insulin, or with the combinations of these hormones. Effect of the combined treatments on the production of serotonin (5HT), or adrenocorticotropic hormone (ACTH) or triiodothyronine (T3) by the cells was compared to the effect of single-hormone treatments. Significant differences were seen between the results obtained following the single or combined treatments. There was no summation of the effects, however an elevation or diminution of the hormone production was observed after the combined treatment, as compared with the... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4338937 Wed, 01 Dec 2010 00:00:00 +0100 4338937 http://www.medworm.com/index.php?rid=4338936&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21183425%26dopt%3DAbstract We report a patient with community acquired Legionella pneumonia, complicated with pulmonary embolism. The patient was cured by levofloxacin and anticoagulant therapy. We performed laboratorial control and we excluded the thrombophilia and antiphospholipid syndrome. Our case report draws attention to the possible association between Legionella infection and pulmonary embolism. PMID: 21183425 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4338936 Wed, 01 Dec 2010 00:00:00 +0100 4338936 http://www.medworm.com/index.php?rid=4338935&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21183426%26dopt%3DAbstract Authors: Kristóf K, Janik L, Komka K, Harmath A, Hajdú J, Nobilis A, Rozgonyi F, Nagy K, Rigó J, Szabó D The occurrence of Candida spp. was investigated during a three-year period in two neonatal intensive care units, Budapest, Hungary. The species distribution among the 41 analysed cases was the following: C. albicans (30/41, 73%), C. parapsilosis (10/41, 24%) and C. glabrata (1/41, 3%). All of the isolates were susceptible to the tested drugs. There was a significant difference in the birth weight, the gestational age <30 weeks and the occurrence of caesarean section between the C. albicans and the C. parapsilosis groups of the cases. Respiratory tract colonization was the same (76-77%) in the extremely low birth weight (ELBW) and the very low birth weight (VLBW) groups. Compa... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4338935 Wed, 01 Dec 2010 00:00:00 +0100 4338935 http://www.medworm.com/index.php?rid=4338934&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21183427%26dopt%3DAbstract This study indicates that ASPP-2 has a specific distribution pattern within tissues and cells in a way that appears to be related to differentiation. However, the patterns are neither simplistic nor straightforward and will require further investigation in order to appreciate fully their physiological/pathological significance. PMID: 21183427 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4338934 Wed, 01 Dec 2010 00:00:00 +0100 4338934 http://www.medworm.com/index.php?rid=4338933&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21183428%26dopt%3DAbstract Authors: Mahdi A, Mohammad N, Morovat T, Mohammad MF, Namam AA, Setareh S, Mohammad E, Amir A, Abbas M, Ali H PMID: 21183428 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4338933 Wed, 01 Dec 2010 00:00:00 +0100 4338933 http://www.medworm.com/index.php?rid=4004200&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20870588%26dopt%3DAbstract Authors: Dombrádi Z, Bihari Z, Horváth KI, Szabó J The performance of the VITEK 2 System (bioMérieux) version 3.01 software was compared to that of the E-test (AB Biodisk, Solna, Sweden) for antibiotic susceptibility testing of 17 clinical isolates of vancomycin resistant enterococcus (VRE). Antibiotic Susceptibility Testing (AST) by VITEK 2 produced 10 minor (59%) errors, resulting in false phenotypes. Reporting of vancomycin resistance in enterococcal strains has enormous therapeutic and epidemiological consequences. Therefore, at laboratories using automated systems (e.g. VITEK 2) for routine microbiological susceptibility testing data must be confirmed by independent validated methods, e.g. E-test, or microdilution. PMID: 20870588 [PubMed - in process] (Source: Acta... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4004200 Tue, 31 Aug 2010 23:00:00 +0100 4004200 http://www.medworm.com/index.php?rid=4004163&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20870589%26dopt%3DAbstract In this study, the occurrence of Pseudomonas aeruginosa and Legionella spp. was examined with taxon-specific PCRs in samples taken at ten points of a municipal drinking water supply system in three months. Sequence analysis confirmed the positivity of samples and revealed a diverse community of legionellae. The results showed that chlorination was an important and effective disinfection method against pathogenic bacteria in drinking water, but pathogenic bacteria could reoccur in the system farther away from the chlorination point. No strong correlation was found between the presence of the investigated potentially pathogenic bacteria and the measured abiotic and biotic parameters within the investigated range. It is hypothesized that instead of physicochemical parameters, the main factors... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4004163 Tue, 31 Aug 2010 23:00:00 +0100 4004163 http://www.medworm.com/index.php?rid=4004121&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20870590%26dopt%3DAbstract Authors: Niller HH It is proposed that hemophagocytic lymphohistiocytosis (HLH) and myelodysplastic syndromes (MDS) may be temporally distinct phases of pathophysiologically related disease processes. A significant subgroup of MDS may develop from subclinical HLH. In that case, HLH-like disease would chronically proceed with little disease activity or under occasional flares only, until it first becomes clinically apparent at the MDS stage. At the MDS stage, however, HLH activity may be easily overlooked by histological or cytogenetic means, since hemophagocytosis has fallen already largely silent. Current treatment options for HLH, like high-dose intravenous immunoglobulins (IVIG), may turn out to be helpful in MDS patients as well.In rare and extreme cases, Leptospira infection cause... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4004121 Tue, 31 Aug 2010 23:00:00 +0100 4004121 http://www.medworm.com/index.php?rid=4004079&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20870591%26dopt%3DAbstract Authors: Verma ML, Kanwar SS A low molecular mass alkaliphilic extra-cellular lipase of Bacillus cereus MTCC 8372 was purified 35-fold by hydrophobic interaction (Octyl-Sepharose) chromatography. The purified enzyme was found to be electrophoretically pure by denaturing gel electrophoresis and possessed a molecular mass of approximately 8 kDa. It is a homopentamer of 40 kDa as revealed by native-PAGE. The lipase was optimally active at 55 °C and retained approximately half of its original activity after 40 min incubation at 55 °C. The enzyme was maximally active at pH 8.5. Mg 2+ , Cu 2+ , Ca 2+ , Hg 2+ , Al 3+ and Fe 3+ at 1 mM enhanced hydrolytic activity of the lipase. Interestingly, Hg 2+ ions synergized and Zn 2+ and Co 2+ ions antagonized the lipase activity. Among surfactan... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4004079 Tue, 31 Aug 2010 23:00:00 +0100 4004079 http://www.medworm.com/index.php?rid=4004026&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20870592%26dopt%3DAbstract Authors: Nweze EI, Okafor JI In vitro susceptibilities of Scedosporium apiospermum isolates recovered from clinical and environmental samples in Nigeria were tested against ten conventional antifungal agents, namely: amphotericin B, nystatin, flucytosine, itraconazole, posaconazole, fluconazole, voriconazole, micafungin, ketoconazole, and terbinafine using the CLSI M38-A broth dilution reference method. The isolates showed varied response/sensitivities to the antifungal agents tested. This is probably the first documented testing of Scedosporium apiospermum isolates from Africa in general and Nigeria in particular against a broad range of conventional antifungal agents. PMID: 20870592 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4004026 Tue, 31 Aug 2010 23:00:00 +0100 4004026 http://www.medworm.com/index.php?rid=4003969&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20870593%26dopt%3DAbstract Authors: Mahapatra S, Banerjee D Endophytic fungi of three tissues (petiole, bark and leaf) of Alstonia scholaris were assessed. A total number of 1,152 endophytic fungi were isolated from 1,002 different plant segments of seven different localities of Paschim Medinipur, West Bengal, India. The isolated fungi belong to nineteen genera, including four unidentified fungi and yeast. Colletotrichum sp. (20.39%) and Sordaria sp. (29.68%) were most commonly isolated from this plant. Hyalopus sp., Fusarium sp. and Curvularia sp. were also isolated. The colonization frequency of endophytic fungi is much higher in leaves (44.66%) in comparison to petioles (32.16%) and barks (23.17%). The study provided evidence for tissue specificity of endophytic fungi. The endophytic fungal species diversity ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4003969 Tue, 31 Aug 2010 23:00:00 +0100 4003969 http://www.medworm.com/index.php?rid=4003908&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20870594%26dopt%3DAbstract In this study, 210 positive blood cultures were tested with FISH method and the results were evaluated comparing to the traditional cultivation based results. Overall agreement between FISH and conventional identification was 91.4%, with better results for Gram-negative bacteria than for Gram-positives (100% and 89.5%, respectively). FISH results were obtained within 1 hour. FISH may serve as a useful tool to supplement traditional microbiological methods for rapid, provisional identification of sepsis pathogens. PMID: 20870594 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4003908 Tue, 31 Aug 2010 23:00:00 +0100 4003908 http://www.medworm.com/index.php?rid=4003836&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20870595%26dopt%3DAbstract Authors: Kádár B, Szász M, Kristóf K, Pesti N, Krizsán G, Szentandrássy J, Rókusz L, Nagy K, Szabó D The aim of the study was to investigate the biofilm-production of 60 Pseudomonas aeruginosa strains isolated from clinical samples and to examine the effect of different antimicrobials and their combinations with clarithromycin on biofilm-formation.The minimal inhibitory concentrations (MICs), minimal biofilm inhibitory concentrations (MBICs), and antibiotic synergy by calculating the fractional inhibitory concentration (FIC) index were determined for the following antibiotics: ceftazidime, cefepime, piperacillin/tazobactam, imipenem, meropenem, levofloxacin, ciprofloxacin, gentamicin, amikacin, tobramycin, netilmicin and clarithromycin.A total of 14 (23.3%) isol... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4003836 Tue, 31 Aug 2010 23:00:00 +0100 4003836 http://www.medworm.com/index.php?rid=4003624&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20870596%26dopt%3DAbstract Authors: Füzi M PMID: 20870596 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=4003624 Tue, 31 Aug 2010 23:00:00 +0100 4003624 http://www.medworm.com/index.php?rid=3720402&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20587381%26dopt%3DAbstract Authors: Berencsi G, Kapusinszky B, Rigó Z, Szomor K Viral interference was discovered about 60 years ago. Molecular epidemiology revealed that this phenomenon possesses important biological implications, it can reduce the epidemic spread of certain viruses from time to time (influenza and enteroviruses) and the efficiency of live vaccination can be impaired, too. Phenomena observed during the last 80 years in Hungary are analyzed. It is suggested to concentrate the distribution of MMR vaccines to seasons of limited influenza and enterovirus circulation. Interference seems to impair the progress of wild poliovirus eradication in the endemic tropical countries. It is recommended to enhance enterovirus surveillance in the region of European countries, since the exchange of the oral po... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3720402 Mon, 31 May 2010 23:00:00 +0100 3720402 http://www.medworm.com/index.php?rid=3720401&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20587382%26dopt%3DAbstract This study shows that the demonstration of the bla OXA-51-like gene is a reliable and rapid way for the presumptive identification of A. baumannii and reveals that the rate of antibiotic resistance is high in Iranian A. baumannii isolates to a variety of antibiotics. PMID: 20587382 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3720401 Mon, 31 May 2010 23:00:00 +0100 3720401 http://www.medworm.com/index.php?rid=3720400&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20587383%26dopt%3DAbstract This study investigated the effect of combination of low pH and some preservatives (e.g. sodium chloride, potassium sorbate or Thymus vulgaris extract) on the growth and caseinase production of Pseudomonas aeruginosa and E. coli at 31 degrees C and 7 degrees C. At 31 degrees C, although growth of the strains was not affected by low pH (5.7) alone, caseinase production by both strains was decreased. A combination of low pH and NaCl, potassium sorbate or thyme extract significantly reduced (p < 0.05) growth and caseinase production by E. coli 28 at 31 degrees C. For P. aeruginosa , addition of NaCl or thyme extract to nutrient broth (low pH) did not affect the growth but reduced caseinase production of the tested strain. Combination of low temperature (7 degrees C) and growth in nutrient ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3720400 Mon, 31 May 2010 23:00:00 +0100 3720400 http://www.medworm.com/index.php?rid=3720399&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20587384%26dopt%3DAbstract Authors: Máthé I, Táncsics A, György E, Pohner Z, Vladár P, Székely AJ, Márialigeti K Water samples of ten mineral water springs at Miercurea Ciuc (Csíkszereda) region (Romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. The results of standard hygienic bacteriological tests showed that the Hargita Spring had perfect and five other springs had microbiologically acceptable water quality (Zsögöd-, Nagy-borvíz-, Taploca-, Szentegyháza- and Lobogó springs). The water of Borsáros Spring was exceptionable (high germ count, presence of Enterococcus spp.).Both standard bacteriological and molecular microbiological methods indicated that the microbiological water quality of the Szeltersz-, Nádasszék- and D�... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3720399 Mon, 31 May 2010 23:00:00 +0100 3720399 http://www.medworm.com/index.php?rid=3720398&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20587385%26dopt%3DAbstract Authors: El-Masry S, Lotfy M, Samy M, Moawia S, El-Sayed IH, Khamees IM Matrix metalloproteinases (MMPs) constitute a large family of enzymes that degrade extracellular matrix proteins (ECM). MMPs are implicated in different pathological conditions such as cancer. Bcl-2 and P53 are key controllers of programmed cell death (PCD) or apoptosis. The aim of the present study was to determine the MMP-9, P53 and Bcl-2 levels in Egyptian patients with Mycobacterium tuberculosis (MTB) (Group I) compared with healthy control individuals (Group II). The concentrations of serum MMP-9 were determined quantitatively using enzyme immunoassay (EIA). P53 and Bcl-2 levels were assayed by flow cytometric analysis using specific monoclones. MMP-9 level was significantly higher in MTB patients compared wit... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3720398 Mon, 31 May 2010 23:00:00 +0100 3720398 http://www.medworm.com/index.php?rid=3720397&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20587386%26dopt%3DAbstract Authors: Pratush A, Seth A, Bhalla TC Rhodococcus rhodochrous PA-34 has been reported to produce nitrile hydratase enzyme that converts 3-cyanopyridine to nicotinamide. A mutant of R. rhodochrous PA-34 was generated through chemical mutagenesis using N-methyl-N-nitro-N-nitrosoguanidine (MNNG) that exhibited 2 times higher nitrile hydratase activity as compared to wild strain. The reaction conditions using resting cells of this mutant strain for the conversion of nicotinamide were optimized. Under the optimized reaction conditions the mutant strain exhibited maximum nitrile hydratase activity [7.8 U/mgdcm (milligram dry cell mass)] at 55 degrees C in 0.3 M potassium phosphate buffer (pH 5.5). PMID: 20587386 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3720397 Mon, 31 May 2010 23:00:00 +0100 3720397 http://www.medworm.com/index.php?rid=3720396&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20587387%26dopt%3DAbstract Authors: Prochnau D, Rödel J, Prager K, Kuersten D, Heller R, Straube E, Figulla HR Microorganisms such as Chlamydia pneumoniae have been shown to infect vascular cells and are believed to contribute to vascular inflammation and atherosclerotic plaque development. Plasma levels of oxidized low density lipoprotein (oxLDL) have received considerable attention as potential predictors of prognosis in atherosclerotic diseases. Lectin-like oxidized LDL receptor-1 (LOX-1) is one of the major receptors for oxidized LDL. It was investigated whether C. pneumoniae infection can stimulate expression of LOX-1 in vascular smooth muscle cells. Expression of LOX-1 in VSMC was measured by RT-PCR and immunoblotting following C. pneumoniae infection. To examine the pharmacological effect of a HMG-CoA ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3720396 Mon, 31 May 2010 23:00:00 +0100 3720396 http://www.medworm.com/index.php?rid=3427595&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20350875%26dopt%3DAbstract Authors: Ivády B Lower respiratory tract infections are among the most important causes of childhood mortality worldwide, more than 2 million children die due to pneumonia every year. A number of infections caused by the main pathogens related to pneumonia can be prevented through vaccination ( S. pneumoniae, H. influenzae type-b, morbilli, pertussis, influenza). In the last decade, after the introduction of the 7-valent pneumococcal conjugated vaccine (PCV), the epidemiological background of childhood pneumonia has changed. Recently, several studies have been performed to collect data and evidences about the efficacy of PCV against noninvasive pneumococcal diseases (e.g. pneumonia, otitis media). These investigations showed 10-50% decrease of all pneumonia cases, 10-30% decrease ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3427595 Mon, 01 Mar 2010 00:00:00 +0100 3427595 http://www.medworm.com/index.php?rid=3427590&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20350876%26dopt%3DAbstract Authors: Yang EJ, Kim SS, Moon JY, Oh TH, Baik JS, Lee NH, Hyun CG A number of essential oils from citrus peels are claimed to have biological activities. Citrus peel, called 'Jin-Pi', is used in traditional medicine for digestion, severe cold, and fever. However, the antibacterial activities against skin pathogens and anti-inflammatory effects of the essential oils of Citrus sunki (JinGyul) and Fortunella japonica var. margarita (GumGyul) have not yet been described. Therefore, in this study, the essential oils of the citrus species C. sunki (CSE) and F. japonica var. margarita (FJE), both native to the island of Jeju, Korea, were examined for their anti-inflammatory and antimicrobial activities against skin pathogens. Four human skin pathogenic microorganisms, Staphylococcus epidermi... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3427590 Mon, 01 Mar 2010 00:00:00 +0100 3427590 http://www.medworm.com/index.php?rid=3427589&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20350877%26dopt%3DAbstract In conclusion, these findings raise the possibility of using serum TIMP-1 as a non-invasive assay in liver fibrosis. Further, the altered balance between circulating MMP-9 and TIMP-1 during HCV infection may play an important role in aggravating liver injury progression in chronic liver diseases. PMID: 20350877 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3427589 Mon, 01 Mar 2010 00:00:00 +0100 3427589 http://www.medworm.com/index.php?rid=3427588&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20350878%26dopt%3DAbstract Authors: Szilágyi E, Füzi M, Damjanova I, Böröcz K, Szonyi K, Tóth A, Nagy K Fourteen outbreaks in Hungary between 2005 and 2008 caused by extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBL-KP) were epidemiologically investigated and the isolated pathogens were characterized by molecular techniques. Ten of the fourteen outbreaks occurred in adult wards and four in neonatal units affecting a total number of 73 patients. The 54% [40] of the patients developed bloodstream infections and 21.9%-21.9% [16] pneumonia and surgical site infections, respectively. The overall rate of mortality proved high: 36.9% [27]. Outbreaks in adults affected more patients, had higher attack rates, were more prolonged in duration and had a 6.9-fold higher mortality... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3427588 Mon, 01 Mar 2010 00:00:00 +0100 3427588 http://www.medworm.com/index.php?rid=3427587&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20350879%26dopt%3DAbstract Monitoring of drug resistance in therapy-naïve HIV infected patients and detection of African HIV subtypes in Hungary. Acta Microbiol Immunol Hung. 2010 Mar;57(1):55-68 Authors: Kanizsai S, Ghidán A, Ujhelyi E, Bánhegyi D, Nagy K Mutations in the HIV-1 pol gene associated with resistance to antiretroviral drugs in therapy-naïve Hungarian individuals transmitted as primary infection by their foreign sexual partners originated from African, Asian and other European countries had been analyzed. Drug resistance genotyping of HIV RT and PR genes were performed where mutations of 72 codons - among them 64 specific resistance codons representing 6 nucleoside reverse transcriptase inhibitor (NRTIs), 2 non-nucleoside reverse transcriptase inhibitor (NNRTIs) and 6 pro... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3427587 Mon, 01 Mar 2010 00:00:00 +0100 3427587 http://www.medworm.com/index.php?rid=3427586&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20350880%26dopt%3DAbstract Authors: Jargin SV PMID: 20350880 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3427586 Mon, 01 Mar 2010 00:00:00 +0100 3427586 http://www.medworm.com/index.php?rid=3427585&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20350881%26dopt%3DAbstract Joseph G. Sinkovics Cytolytic Immune Lymphocytes In the Armamentarium of the Human Host Products of the Evolving Universal Immune System Schenk Verlag - Dialóg Campus - ISBN 978-3-939337-57-7. Acta Microbiol Immunol Hung. 2010 Mar;57(1):71-2 Authors: Nagy K PMID: 20350881 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3427585 Mon, 01 Mar 2010 00:00:00 +0100 3427585 http://www.medworm.com/index.php?rid=3061704&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19957405%26dopt%3DAbstract Authors: PMID: 19957405 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=3061704 Mon, 07 Dec 2009 00:54:03 +0100 3061704 http://www.medworm.com/index.php?rid=2902146&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19828428%26dopt%3DAbstract Authors: PMID: 19828428 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2902146 Wed, 30 Sep 2009 23:00:00 +0100 2902146 http://www.medworm.com/index.php?rid=2856142&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789136%26dopt%3DAbstract Authors: Ongrádi J, Stercz B, Kövesdi V, Vértes L The sharp increase of life expectancy and the increasing ratio of ageing population pose new challenges for the public health system. The elderly suffer from more frequent and severe infections than young people. Theoretically, vaccination could protect the elderly against several infectious diseases, but due to their age-related immune impairment, vaccination might fail in many cases. Instead of ineffective vaccination campaigns, exploration and restoration of age-dependent dysregulation of their immune functions have to be placed into the focus of recent research. Frequent comorbidities in these people augment immune defects. Immunosenescence affects both the innate and adaptive immunity. Disturbances in macrophage-deri... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2856142 Mon, 31 Aug 2009 23:00:00 +0100 2856142 http://www.medworm.com/index.php?rid=2856141&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789137%26dopt%3DAbstract Authors: Abo-Amer AE, Altalhi AD The dcuSR operon of Escherichia coli encodes a two-component sensor/kinase-response/regulator system. This system regulates gene expression in response to external C 4 -dicarboxylates. During entry into stationary phase Gram-negative bacteria express genes that impart cellular resistance to environmental stresses. In E. coli , 50 or more genes are triggered by sigma factor ( sigma s ) during entry into stationary phase. Multi-copy dcuS-lacZ and chromosomally integrated dcuS-lacZ fusions analysis showed that the expression of dcuSR is positively regulated during growth phase. Many genes that are required for stationary-phase adaptation are controlled by RpoS, a conserved alternative sigma factor, whose expression is, in turn, controlled by many factors. ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2856141 Mon, 31 Aug 2009 23:00:00 +0100 2856141 http://www.medworm.com/index.php?rid=2856140&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789138%26dopt%3DAbstract Authors: Verma ML, Azmi W, Kanwar SS A wide range of fatty acid esters can be synthesized by esterification and transesterification reactions catalyzed by lipases in non-aqueous systems. In the present study, immobilization of a purified alkaline extra-cellular lipase of Bacillus cereus MTCC 8372 by adsorption on diatomaceous earth (celite) for synthesis of ethyl acetate via transesterification route was investigated. B. cereus lipase was deposited on celite (77% protein binding efficiency) by direct binding from aqueous solution. Immobilized lipase was used to synthesis of ethyl acetate from vinyl acetate and ethanol in n -nonane. Various reaction conditions, such as biocatalyst concentration, substrates concentration, choices of solvents ( n -alkanes), incubation time, temperature, m... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2856140 Mon, 31 Aug 2009 23:00:00 +0100 2856140 http://www.medworm.com/index.php?rid=2856139&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789139%26dopt%3DAbstract Authors: Banerjee D, Manna S, Mahapatra S, Pati BR Three medicinal plants Ocimum sanctum, Ocimum bacilicum and Leucas aspera were screened to study endophytic diversity of the plants. Altogether 103 fungal endophytes belonging to fourteen genera were isolated. Leaves of all three medicinal plants were colonized by a great number of endophytic fungi. Leaves of O. sanctum were colonized by the most, that is, eleven endophytes. Highest Shannon-Wiener index (2.256) was exhibited by O. sanctum with the highest Simpson's diversity (0.8654) indicating great species specificity. O. bacilicum and L. aspera showed the highest similarity coefficient. Some fungal genera have been showed to be host specific. In the present study Curvularia sp., Hymenula sp., Tricoderma sp. and Tubercularia sp. excl... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2856139 Mon, 31 Aug 2009 23:00:00 +0100 2856139 http://www.medworm.com/index.php?rid=2856138&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789140%26dopt%3DAbstract Authors: Szilágyi E, Füzi M, Böröcz K, Kurcz A, Tóth A, Nagy K Risk factors for and outcomes of bloodstream infections (BSIs) caused by ESBL-producing and by ESBL-non-producing Klebsiella pneumoniae were compared in a four-year multicenter study in Hungary. One hundred ESBL-positive and one hundred ESBL-negative patients were included as cases and controls. Investigated risk factors were related to demographics, comorbid conditions, treatments, invasive procedures, surgery prior bacteremia, presence of additional nosocomial infections and preceding hospital admission within a year. Measured outcomes were crude mortality, mortality related to infection and delay in introducing appropriate therapy (DAT). Though some risk factors for infection (admission to intens... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2856138 Mon, 31 Aug 2009 23:00:00 +0100 2856138 http://www.medworm.com/index.php?rid=2856137&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789141%26dopt%3DAbstract Authors: Zaidi A, Khan MS, Ahemad M, Oves M Most agronomic soils contain large reserves of total phosphorus [P], but the fixation and precipitation of P cause P deficiency, and in turn, restrict the growth of crops severely. Phosphorus replenishment, especially in sustainable production systems, remains a major challenge as it is mainly fertilizer-dependent. Though the use of chemical P fertilizers is obviously the best means to circumvent P deficiency in different agro-ecosystems, their use is always limited due to its spiralling cost. A greater interest has, therefore, been generated to find an alternative yet inexpensive technology that could provide sufficient P to plants while reducing the dependence on expensive chemical P fertilizers. Among the heterogeneous and naturally abunda... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2856137 Mon, 31 Aug 2009 23:00:00 +0100 2856137 http://www.medworm.com/index.php?rid=2856136&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789142%26dopt%3DAbstract Authors: Kacániová M, Pavlicová S, Hascík P, Kociubinski G, Kńazovická V, Sudzina M, Sudzinová J, Fikselová M As the honey-bee gastrointestinal tract microflora and pollen are the primary sources for the honey microbial community, the aim of this work was to study and characterize the microbial transit among them. Therefore, an exhaustive microbial analysis of honey, adult honey-bee gastrointestinal tract, and pollen from different Slovakian regions and different seasons, was conducted. Microbial screening revealed that the primary sources of microbial community present in Slovakian honey are pollen and the honey-bees' digestive tract microflora, containing microorganisms normally present in dust, air and flowers. We found that the digestive tra... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2856136 Mon, 31 Aug 2009 23:00:00 +0100 2856136 http://www.medworm.com/index.php?rid=2856135&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789143%26dopt%3DAbstract Authors: Rajcáni J PMID: 19789143 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2856135 Mon, 31 Aug 2009 23:00:00 +0100 2856135 http://www.medworm.com/index.php?rid=2635116&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19621764%26dopt%3DAbstract Authors: Nász I PMID: 19621764 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2635116 Sun, 31 May 2009 23:00:00 +0100 2635116 http://www.medworm.com/index.php?rid=2635115&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19621765%26dopt%3DAbstract This study is an attempt to optimize the cultural conditions for efficient production of P(3HB) by H. marina in batch cultivation. Growth of the organism under shake-flask culture using 2% (w/v) glucose resulted in P(3HB) accumulation accounting for more than 59% of cell dry weight after 50 h of incubation. The optimum P(3HB) production was attained with a combined supply of NH4Cl and yeast extract as N-source, 0.01% (w/v) phosphate, 1.5% (w/v) sulphate and 10% (w/v) NaCl. Qualitative and quantitative 1HNMR and FT-IR analysis of cells grown in alkanoic acids (C3-C6) as sole source of carbon and co-substrates revealed synthesis of PHA co-polymers composed of 3-hydroxybutyric acid and 3-hydroxyvaleric acid [P(3HB-co-3HV)]. In two-step cultivation, accumulation of the co-polymer was significa... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2635115 Sun, 31 May 2009 23:00:00 +0100 2635115 http://www.medworm.com/index.php?rid=2635114&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19621766%26dopt%3DAbstract Authors: Okewole EA, Adejinmi JO The aim of this study was to compare a clinic-based enzyme-linked immunosorbent assay (immunocomb-Eliza, Biogal, Israel) and a field-based dot-blot enzyme-linked immunoassay (DBELIA) with the indirect immunofluoresence antibody test (IFAT) used for the field diagnostics of canine monocytic ehrlichiosis (CME). The total test samples was 60; out of them 52 serum samples came from naturally exposed police German shepherd dogs and 8 from adult mongrels infected with viable Ehrlichia canis propagated in a dog macrophage laboratory cell line (DH82). The DBELIA test was positive in 86.7% (56/60) and negative in 13.3% (8/60), while the immunocomb-Eliza was positive in 91.7% (55/60) and negative in 8.3% (5/60). IFAT revealed 90.0% (54/60) of DBELIA samples as po... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2635114 Sun, 31 May 2009 23:00:00 +0100 2635114 http://www.medworm.com/index.php?rid=2635113&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19621767%26dopt%3DAbstract Authors: Mandal SM, Mandal M, Pati BR, Das AK, Ghosh AK The genus Rhizobium colonizes in leguminous plants by symbiotic relationship and enriches soil nitrogen through the formation of the root nodule. Legumes are the appropriate crops for the recovery of marginal lands and they can easily grow in adverse climatic condition. Rhizobium sp. VMA301 was isolated from the root nodules of V. mungo, grown in arsenic contaminated field. The LC50 value of arsenite for VMA301 was found to be 1.8 mM. Sixteen differentially expressed proteins were identified using RP-HPLC and MALDI ToF mass spectrometry from arsenite induced whole cell lysate soluble proteins. It is also found that nine proteins were up-regulated and seven proteins were down-regulated in comparison to the control group (cells grow... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2635113 Sun, 31 May 2009 23:00:00 +0100 2635113 http://www.medworm.com/index.php?rid=2635112&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19621768%26dopt%3DAbstract Authors: Kaur H, Kaur A, Saini HS, Chadha BS 134 fungal cultures isolated from different soil samples were screened for lovastatin production. Of these, 38 isolates produced different levels of lovastatin. An Aspergillus terreus strain GD13, producing 190 mg/l of lovastatin was selected and subjected to a rational mutation-selection programme based on the resistance to lovastatin and fatty acid synthase (FAS) inhibitors, viz., iodoacetamide and N-ethylmaleimide. After three cycles of mutagenesis, a hyper-producing mutant (EM19) exhibiting 7.5-fold (1424 mg/l) higher levels of lovastatin when compared to wild type parent strain was obtained. PMID: 19621768 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2635112 Sun, 31 May 2009 23:00:00 +0100 2635112 http://www.medworm.com/index.php?rid=2635111&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19621769%26dopt%3DAbstract Authors: Talha E, Juhász E, Kanizsai S, Nagy K For the molecular detection of Treponema pallidum authors introduced and used a nested PCR amplifying a conservative portion of the gene coding for the Tp 47 kDa membrane protein. PCR verified the presence of T. pallidum specific DNA in 5.7 per cent of syphilis seronegative 105 MSM belonging to HIV risk group. Treponema DNA was also detected in HIV infected, syphilis seronegative cases. Specificity of the method was demonstrated in rabbit inoculation test and also in clinically positive syphilis cases. PMID: 19621769 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2635111 Sun, 31 May 2009 23:00:00 +0100 2635111 http://www.medworm.com/index.php?rid=2635110&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19621770%26dopt%3DAbstract Authors: Suyarsono , Budyani DR, Sosroseno W The aim of the present study was to determine the expression ofgranzyme B in non-perforated appendicitis. Appendix biopsies were obtained from the patients with clinically diagnosed as acute appendicitis and subjects admitted for elective abdominal surgery. All biopsies from the patients were non-perforated and histologically divided into acute and non-acute appendicitis. Granzyme B expression was assessed immunohistochemically. The results showed that granzyme B expression in both acute and non-acute appendicitis was significantly lower than that in the control appendix tissues (P < 0.05). The expression of this cytotoxic protein in acute and non-acute appendicitis was comparable (P > 0.05). Therefore, the results of the present study... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2635110 Sun, 31 May 2009 23:00:00 +0100 2635110 http://www.medworm.com/index.php?rid=2473847&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19388554%26dopt%3DAbstract Authors: Minárovits J It is well documented that the double-stranded DNA (dsDNA) genomes of certain viruses and the proviral genomes of retroviruses are regularly targeted by epigenetic regulatory mechanisms (DNA methylation, histone modifications, binding of regulatory proteins) in infected cells. In parallel, proteins encoded by viral genomes may affect the activity of a set of cellular promoters by interacting with the very same epigenetic regulatory machinery. This may result in epigenetic dysregulation and subsequent cellular dysfunctions that may manifest in or contribute to the development of pathological changes (e.g. initiation and progression of malignant neoplasms; immunodeficiency). Bacteria infecting mammals may cause diseases in a similar manner, by causing hypermeth... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2473847 Sun, 01 Mar 2009 05:00:00 +0100 2473847 http://www.medworm.com/index.php?rid=2473846&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19388555%26dopt%3DAbstract Authors: Kristóf K, Kocsis E, Nagy K Prematurity has got special challenge for clinicians and also other medical staff, such as microbiologists. Immature host defense mechanisms support early-onset sepsis, which can be very serious with very high mortality. While the past decade has been marked by a significant decline in early-onset group B streptococcal (GBS) sepsis in both term and preterm neonates, the overall incidence of early-onset sepsis has not decreased in many centers, and several studies have found an increase in sepsis due to gram-negative organisms. With increasing survival of these more fastidious preterm infants, late-onset sepsis or specially nosocomial bloodstream infection (BSI) will continue to be a challenging complication that affects other morbidities, lengt... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2473846 Sun, 01 Mar 2009 05:00:00 +0100 2473846 http://www.medworm.com/index.php?rid=2473845&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19388556%26dopt%3DAbstract Authors: Füzi M, Horváth P Until recently the etiology of bovine spongiform encephalopathy (BSE) was considered uniform. The infectious agent was thought to be a single strain of prion (posttranslationally altered form of normal prion protein: PrPSc) retaining its biochemical and biological characteristics during interspecies transmission. However, alternate PrPSc signatures through large-scale screening have recently been detected. In addition, genetic alterations governing susceptibility to prion infection and a mutation (E211K) capable of eliciting spontaneous BSE have been demonstrated. Thus, the spectrum of BSEs have broadened and three PrPSc variants (BSE-C, BSE-H and BSE-L) are now defined. Moreover, a new condition resembling BSE, idiopathic brainstem neuronal chromat... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2473845 Sun, 01 Mar 2009 05:00:00 +0100 2473845 http://www.medworm.com/index.php?rid=2473844&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19388557%26dopt%3DAbstract Authors: Essien JP, Umoh AA, Akpan EJ, Eduok SI, Umoiyoho A Mycological research was conducted on the mycelial growth, keratinolytic proteinase activity and thermotolerance ofdermatophytes associated with alopecia patients in Uyo, Nigeria. The results revealed that Microsporum sp. - AP1, Epidermophyton sp. - AP2, Trichophyton rubrum - AP4, Trichophyton mentagrophytes - AP5 and a yeast Candida albicans - AP3 isolated exhibited variable growth and keratinase activity at different temperatures. Microsporum sp. - AP1 and T. mentagrophytes - AP5 survived heat treatment at 90 degrees C but exhibited best mycelial growth at 30 degrees C (with 53.41 mg/50 ml biomass dry weight) and 40 degrees C (with 61.32 mg/50 ml biomass dry weight) respectively, after incubation for 2 weeks. Trichophyton ru... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2473844 Sun, 01 Mar 2009 05:00:00 +0100 2473844 http://www.medworm.com/index.php?rid=2473843&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19388558%26dopt%3DAbstract Authors: Essien JP, Jonah I, Umoh AA, Eduok SI, Akpan EJ, Umoiyoho A The incidence and heat resistance of conidiospores produced by dermatophytes isolated from athlete's kits (canvasses, stockings and spike shoes) stored in Nigerian University Sport's Centre were investigated. Epidermophyton floccosum, Microsporum oudouinii, Microsporum canis, Trichophyton concentricum, Trichophyton mentagrophytes and Trichophyton rubrum were isolated and their incidence on the athlete's kits varied with the species and type of kits. Among the isolates T. mentagrophytes, T. rubrum and E. floccosum with 25%, 23% and 20% prevalence rates respectively, were the most common isolates, and are often associated with tinea pedis (athletes foot). Canvasses with the highest incidence of dermatophytes (25 out of ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2473843 Sun, 01 Mar 2009 05:00:00 +0100 2473843 http://www.medworm.com/index.php?rid=2473842&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19388559%26dopt%3DAbstract Authors: Ali S, Hovenden JL, Symon DN Sixty-nine children were identified and evaluable. Forty-one (60%) presented with hypotension and/or abnormal neurological signs. In 34 (49%) a petechial rash was present on admission. Of note, 13 (19%) had a non-petechial rash, and rash was absent in 19 (28%). Twenty-one (30%) presented with meningism or meningitis. In one child the illness was so mild that the child was discharged prior to making a diagnosis. Five children died (7%). Sixty-three cases (91%) were diagnosed by blood or cerebrospinal fluid culture; these investigations remain the mainstay of diagnosis. PMID: 19388559 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2473842 Sun, 01 Mar 2009 05:00:00 +0100 2473842 http://www.medworm.com/index.php?rid=2473833&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19388560%26dopt%3DAbstract This study was conducted at a 900+ bed general teaching hospital, from May to September 2007, in Iran. The aim of this study was to determine the prevalence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae and their antimicrobial pattern. The Kirby-Bauer disk diffusion method and the phenotypic disk confirmatory test were performed for each isolate. The total of 206 isolates including 106 E. coli and 100 K. pneumoniae were collected of which 122 isolates (59.2%) were ESBL positive. The prevalence of ESBL-producing strains was 59.2% (122/206). All the isolates were susceptible to imipenem. Among the ESBL-producing isolates, the sensitivity was from 3.3% to 61.5% for ampicillin to aztreonam. From female isolates (136), 59.5% and from male isolat... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2473833 Sun, 01 Mar 2009 05:00:00 +0100 2473833 http://www.medworm.com/index.php?rid=2473832&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19388561%26dopt%3DAbstract Authors: Thakur M, Azmi W The potential of pyridoxal-5-phosphate dependent tyrosine decarboxylase (E.C. 4.1.1.25) of Lactococcus lactis was explored for the biotransformation of L-tyrosine to tyramine. Maximum bioconversion of L-tyrosine to tyramine was achieved in tyramine production medium (pH -5.5) at 30 degrees C after 16 h of incubation with 0.2% L-tyrosine. The yield oftyramine was found to be 11.8 microg/mL by the growing cells of L. lactis at shake flask level. Growth medium and different physico-chemical parameters to maximize the biotransformation of L-tyrosine to tyramine were optimized and yielded 1.9-fold increased synthesis of tyramine. PMID: 19388561 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2473832 Sun, 01 Mar 2009 05:00:00 +0100 2473832 http://www.medworm.com/index.php?rid=2095396&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130744%26dopt%3DAbstract Authors: Nagy K PMID: 19130744 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095396 Mon, 01 Dec 2008 05:00:00 +0100 2095396 http://www.medworm.com/index.php?rid=2095395&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130745%26dopt%3DAbstract Authors: Sinkovics JG The author of the monograph "Cytolytic Immune Lymphocytes..." (published in 2008 by Schenk Buchverlag Campus Dialog, Budapest, Passau, Pécs) proposed several research projects and described certain clinical events that require further elaboration and documentation. In this article the author provides what is required and has since become available. The first subject matter in question concerns the fusogenic viruses. The ancient fusogenic viruses might have created the first eukaryotic cell(s) by uniting archaeabacterial and prokaryotic/protobacterial protospheroplasts. Extant fusogenic viruses either produce tumor cell syncytia and lyse them, thus practicing viral oncolysis. Or, create chimaeric fusion products, the so-called "natural hybridomas", of lymphoma... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095395 Mon, 01 Dec 2008 05:00:00 +0100 2095395 http://www.medworm.com/index.php?rid=2095394&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130746%26dopt%3DAbstract Authors: Juhász E, Ghidán A, Kemény B, Nagy K Mutations in the HIV-1 genes associated with resistance to antiretroviral drugs were detected also in primary HIV infected individuals who did not receive antiretroviral treatment. Drug resistance genotyping of HIV pol gene was done by in situ DNA hybridization using a Line Probe Assay and by direct sequencing. Viral variants harbouring resistance mutations such as: M41, T69R, K70R, M184V, T215Y in the pol gene were detected in 14% of the subjects. HIV mutants resistant to NRT inhibitors were found in 10 and 20% of patients infected before and after the year 2000, respectively. Multiple drug resistant viruses (2-3 drug classes) were present in 3.5% of the mainly recently infected patients. In protease gene only minor resistan... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095394 Mon, 01 Dec 2008 05:00:00 +0100 2095394 http://www.medworm.com/index.php?rid=2095393&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130747%26dopt%3DAbstract In this study we summarise the results of the first major epidemiological survey of Streptococcus pneumoniae isolates from Hungary. Neither the incidence nor the level of resistance to penicillin of the strains was high, we observed, however, 40% macrolide resistance. The main underlying mechanism was the presence of the erm(B) gene, but we could find also strains with the mef gene, some of them with unusually high resistance levels. The main serogroups in ranking order were 6, 9, 19, 23 and 14, accounting together for 88.2% of all typed strains. We found a close correlation between serotypes and resistance. As a result of genotyping the penicillin non-susceptible strains, we found that the penicillin resistant isolates all belonged to the same PFGE clone, while in the intermediate group t... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095393 Mon, 01 Dec 2008 05:00:00 +0100 2095393 http://www.medworm.com/index.php?rid=2095392&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130748%26dopt%3DAbstract In this report we examined the glycopeptide susceptibility of enterococci, isolated in 2005, from slaughtered animals, within the confines of Hungarian Antibiotic Resistance Monitoring System. We determined the presence of the van genes as well as their genetic relatedness in enterococci from poultry. Enterococcus sp. strains (n=175) were collected from intestinal samples of slaughtered poultry in 2005. The origin of the samples was registered at county level. After screening the strains with 30 mg vancomycin disc 19 (86%) intermediate resistant and 4 (3%) fully resistant strains were found. The distribution of minimum inhibitory concentration (MIC)-values among 23 enterococcus strains which were intermediate or resistant to vancomycin were 0.25 mg/L (4.4%), 2 mg/L (8.6%), 4 mg/L (8.6%), 8... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095392 Mon, 01 Dec 2008 05:00:00 +0100 2095392 http://www.medworm.com/index.php?rid=2095391&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130749%26dopt%3DAbstract Authors: Herczegh A, Ghidán A, Deseo K, Kamotsay K, Tarján I A study was conducted to compare the DNA structure of Streptococcus mutans strains in children with caries-active, caries-free, and gingivitis clinical diagnosis. Twenty-eight Streptococcus mutans strains from 100 children's plaques were examined by pulsed-field gel electrophoresis (PFGE) method. The classified strains were closely related to one another, though the strains originated from different disease groups. Three identical pairs were found, but the pairs in two cases belonged to different disease groups. The results of the PFGE experiments suggest that there is no correlation between the different DNA patterns ofS. mutans strains and their cariogenecity. So the different DNA strains ofS. mutans are not the o... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095391 Mon, 01 Dec 2008 05:00:00 +0100 2095391 http://www.medworm.com/index.php?rid=2095390&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130750%26dopt%3DAbstract Authors: Hettmann A, Gerle B, Barcsay E, Csiszár C, Takács M Herpes simplex virus type 2 infection is a quite common but frequently asymptomatic, therefore undiagnosed condition. Genital HSV-2 infection may cause neonatal herpes, enhances HIV transmission and may play a role in infertility. To evaluate the prevalence of HSV-2 in Hungary we tested 2500 serum samples for the presence of anti-HSV-2 IgG by ELISA method. According to our results Hungary belongs to the low-infected countries, the HSV-2 seroprevalence grows with age and is significantly higher among women than in men. We also examined the serostatus of 512 pregnant women and 539 women attending infertility clinics. Results show that the HSV-2 prevalence is significantly higher among women attending infertility clini... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095390 Mon, 01 Dec 2008 05:00:00 +0100 2095390 http://www.medworm.com/index.php?rid=2095389&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130751%26dopt%3DAbstract Authors: Sasvári Z, Posta K, Hornok L Expression patterns of cel5A and cel5B, two endoglucanase encoding genes of Thermobifida fusca were compared by quantitative real-time PCR. With Avicel as carbon source the transcript level of cel5A continuously increased until the 10th hour of incubation and then a sharp decrease was observed, whereas cel5B presented a slow constitutive expression on this substrate. When the microcrystalline cellulose powder MN300 was used as the inducing carbon source, the expression patterns of the two genes were similar. A low initial level of expression was followed by a rapid increase at the 5th hour of incubation; a transient repression was then observed at the 10th hour but after this sampling time, the expression levels started to increase again. The ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095389 Mon, 01 Dec 2008 05:00:00 +0100 2095389 http://www.medworm.com/index.php?rid=2095388&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130752%26dopt%3DAbstract In conclusion, this is the first report of VIM MBL-producing P. aeruginosa from Serbia and also of the occurrence of such isolates belonging to the international clonal complex CC11 in this country. PMID: 19130752 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095388 Mon, 01 Dec 2008 05:00:00 +0100 2095388 http://www.medworm.com/index.php?rid=2095387&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19130753%26dopt%3DAbstract In this study, the relationship between five water-quality parameters (water temperature, pH, conductivity, water-soluble oxygen (WSO) and organic matter content (chemical oxygen demand-COD)) and avian botulism cases were examined using statistical methods. Five-five sampling points, located in high and low avian botulism risk (HR and LR) areas of Lake 'Kis-Balaton' were chosen, respectively. Data from 5 HR and 5 LR years were processed. In some cases, significant (P<0.05) differences were discovered in water temperature, pH, WSO and COD values between HR and LR sites and years. Discriminant analysis verified positive relationship between the occurrence of avian botulism outbreaks and characteristics of the sampling sites in 16/20 cases (80.0%). In the remaining instances, the role of o... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=2095387 Mon, 01 Dec 2008 05:00:00 +0100 2095387 http://www.medworm.com/index.php?rid=1810312&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18800594%26dopt%3DAbstract Authors: Verma ML, Azmi W, Kanwar SS In recent times, biotechnological applications of microbial lipases in synthesis of many organic molecules have rapidly increased in non-aqueous media. Microbial lipases are the 'working horses' in biocatalysis and have been extensively studied when their exceptionally high stability in non-aqueous media has been discovered. Stability of lipases in organic solvents makes them commercially feasibile in the enzymatic esterification reactions. Their stability is affected by temperature, reaction medium, water concentration and by the biocatalyst's preparation. An optimization process for ester synthesis from pilot scale to industrial scale in the reaction medium is discussed. The water released during the esterification process can be controlled over a... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1810312 Mon, 01 Sep 2008 04:00:00 +0100 1810312 http://www.medworm.com/index.php?rid=1810311&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18800595%26dopt%3DAbstract Authors: Ndaw A, Zinedine A, Faid M, Bouseta A Lactic acid bacteria (LAB) strains were assayed for the conservation of fresh sardine "Sardina pilchardus". Lactobacillus delbrueckii subsp. delbrueckii was used for inoculation of sardine fillets in a solution of NaCl (5%, w/w) and glucose (4%, w/w) concentration in water. Microbial counts including Standard Plate Count (SPC), LAB, yeasts, coliforms, Salmonella, staphylococci and Clostridium were followed during two weeks of storage at 30 degrees C. Determinations of chemical parameters including pH, dry matter, fat, ash, total nitrogen (NT), total volatile basic nitrogen (TVBN) and trimethylamine (TMA) were carried out under the same conditions. Chemical determinations showed a net pH decrease from an initial value of 6.05 in raw sardine... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1810311 Mon, 01 Sep 2008 04:00:00 +0100 1810311 http://www.medworm.com/index.php?rid=1810310&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18800596%26dopt%3DAbstract This study indicated good quality of tap water and bottled water. The untreated water samples (hand pumps) were, however, slightly contaminated by faecal coliforms, faecal enterococci, Clostridium perfringens, Salmonella and Shigella. Consequently, the consumers in the villages receiving water through hand pumps are often exposed to the risk of water-borne diseases due to inadequate treatment of the raw water. Therefore, there are guidelines necessary to protect groundwater quality. Moreover, PCR-amplified by some functional gene fragments such as dctA, dcuB, frdA, dcuS and dcuR genes of the E. coli was adapted for use as a non-cultivation-based molecular approach for detection of E. coli populations from water samples without the need for pure and identified cultures. PMID: 18800596 [... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1810310 Mon, 01 Sep 2008 04:00:00 +0100 1810310 http://www.medworm.com/index.php?rid=1810309&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18800597%26dopt%3DAbstract Authors: Verma ML, Chauhan GS, Kanwar SS A purified alkaline thermo-tolerant bacterial lipase from Bacillus cereus MTCC 8372 was immobilized on a Poly (MAc-co-DMA-cl-MBAm) hydrogel. The hydrogel showed approximately 94% binding capacity for lipase. The immobilized lipase (2.36 IU) was used to achieve esterification ofmyristic acid and isopropanol in n-heptane at 65 degrees C under continuous shaking. The myristic acid and isopropanol when used at a concentration of 100 mM each in n-heptane resulted in formation of isopropyl myristate (66.0 +/- 0.3 mM) in 15 h. The reaction temperature below or higher than 65 degrees C markedly reduced the formation of isopropyl myristate. Addition of a molecular sieve (3 A x 1.5 mm) to the reaction mixture drastically reduced the ester formation. The h... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1810309 Mon, 01 Sep 2008 04:00:00 +0100 1810309 http://www.medworm.com/index.php?rid=1810308&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18800598%26dopt%3DAbstract In conclusion OPV appeared to be effective in the management of RAS. PMID: 18800598 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1810308 Mon, 01 Sep 2008 04:00:00 +0100 1810308 http://www.medworm.com/index.php?rid=1810307&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18800599%26dopt%3DAbstract Authors: Erdei E, Pusztahelyi T, Miskei M, Barna T, Pócsi I Under carbon starvation, Aspergillus nidulans produced a fungal/bacterial type chitinase, ChiB. The chiB gene was cloned and subcloned into pJC40 expression vector containing a 10XHis fusion tag, and the ChiB protein was expressed heterologously in Escherichia coli. Recombinant and native ChiB enzymes shared the same optimal pH ranges and showed similar substrate specificities with endo-acting cleavage patterns. PMID: 18800599 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1810307 Mon, 01 Sep 2008 04:00:00 +0100 1810307 http://www.medworm.com/index.php?rid=1578328&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18595314%26dopt%3DAbstract Authors: Thevelein JM, Bonini BM, Castermans D, Haesendonckx S, Kriel J, Louwet W, Thayumanavan P, Popova Y, Rubio-Texeira M, Schepers W, Vandormael P, Van Zeebroeck G, Verhaert P, Versele M, Voordeckers K In yeast the Protein Kinase A (PKA) pathway can be activated by a variety of nutrients. Fermentable sugars, like glucose and sucrose, trigger a spike in the cAMP level, followed by activation of PKA and phosphorylation of target proteins causing a.o. mobilization of reserve carbohydrates, repression of stress-related genes and induction of growth-related genes. Glucose and sucrose are sensed by a G-protein coupled receptor system that activates adenylate cyclase and also activates a bypass pathway causing direct activation of PKA. Addition of other essential nutrients, like nitrogen ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1578328 Sun, 01 Jun 2008 04:00:00 +0100 1578328 http://www.medworm.com/index.php?rid=1578327&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18595315%26dopt%3DAbstract Authors: Vánky K After defining the meaning of the term 'smut fungus', their current taxonomic classification is presented. There are to date, 1640 'true' species of smut fungi that are classified into 2 phyla, 2 subphyla, 4 classes, 8 orders, 24 families and 90 genera. Recent changes to the classification of the smut fungi have produced some surprises when compared to their traditional classification. The variability of the symptoms produced by the smut fungi on their host plants, and the great morphological diversity of the spores is illustrated by selected pictures. Trends and perspectives in the research of the taxonomy of smut fungi are discussed. On the occasion of the imminent world monograph of smut fungi the necessity for urgent measures ensuring their global conservation... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1578327 Sun, 01 Jun 2008 04:00:00 +0100 1578327 http://www.medworm.com/index.php?rid=1578326&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18595316%26dopt%3DAbstract Authors: Emri T, Szilágyi M, Justyák A, Pócsi I Extracellular proteinase production induced by carbon starvation was studied in a series of heterotrimeric G protein signaling pathway mutants of Aspergillus nidulans. All the mutants tested--including deltafadA (Galpha), deltasfaD (Gbeta), deltagpgA (Ggamma) and deltasfgA (regulator of FadA signaling)--showed an elevated proteinase production after glucose depletion. Our results strongly support the view that during growth, FadA/SfaD/GpgA G protein signaling inhibits proteinase production via both Galpha and Gbetagamma subunits, and all conditions, which are not sufficient to support vegetative growth and, hence, inhibit this type of G protein signaling, elevate extracellular proteinase activities. PMID: 18595316 [PubM... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1578326 Sun, 01 Jun 2008 04:00:00 +0100 1578326 http://www.medworm.com/index.php?rid=1578325&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18595317%26dopt%3DAbstract Authors: Fekete E, Padra J, Szentirmai A, Karaffa L The disaccharide lactose is a byproduct of cheese production accumulating to amounts of 800,000 tons per year worldwide, of which 15% is used as a carbon source for various microbial fermentations. Nevertheless, little is known about the regulation of its metabolism in filamentous fungi. Lactose is metabolized slowly, and some important fungi such as A. niger cannot use it at all. A more detailed knowledge on the rate-limiting steps would be helpful to improve its industrial application. We have chosen A. nidulans as an object for investigating how lactose and galactose metabolism are regulated because it has long become a model system for biochemical and genetic research on fungi, and mutants in the lactose-metabolizing pathway of A.... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1578325 Sun, 01 Jun 2008 04:00:00 +0100 1578325 http://www.medworm.com/index.php?rid=1578324&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18595318%26dopt%3DAbstract Authors: Kothe E Pheromones have been detected in all fungal phylogenetic lineages. This came as a surprise, as the general role of pheromones in mate attraction was not envisioned for some fungi. Pheromones and pheromone receptor genes have been identified, however, in members of all true fungal lineages, and even for mycelia forming organisms of plant and amoeba lineages, like oomycetes and myxomycetes. The mating systems and genes governing the mating type are different in fungi, ranging from bipolar with two opposite mating types to tetrapolar mating systems (with four possible mating outcomes, only one of which leads to fertile sexual development) in homobasidioymcetes with more than 23,000 mating types occurring in nature. Pheromones and receptors specifically recognizing these p... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1578324 Sun, 01 Jun 2008 04:00:00 +0100 1578324 http://www.medworm.com/index.php?rid=1578323&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18595319%26dopt%3DAbstract Authors: Pap K, Tornai-Lehoczki J, Syposs Z Microbiological challenge study was carried out to verify the microbial stability of bottled waters against four different mold species isolated from bottled water (Fusarium sp.; Cladosporium sp.; Penicillium chrysogenum and Aspergillus fumigatus) and to follow the growth of the molds in bottled water. Twelve types of bottled water with different mineralization and CO2 level in PET and glass packages were collected from 4 European countries. Three different inoculation levels of spore suspensions were used to contaminate bottled water samples. The surviving colony forming unit (CFU) numbers and visual growth were monitored during the investigation period (26 weeks). The results of surviving CFU showed that the fungal growth is mostly determin... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1578323 Sun, 01 Jun 2008 04:00:00 +0100 1578323 http://www.medworm.com/index.php?rid=1578322&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18595320%26dopt%3DAbstract Authors: Elisashvili V, Kachlishvili E, Penninckx MJ Cultivation of two commercial Pleurotus ostreatus (oyster mushroom) strains was performed in plastic bags. Tree leaves appeared to be an excellent growth substrate for the conversion into fruiting bodies with biological efficiency of 108-118%. The level of enzyme activity was strongly regulated during the life cycle of mushrooms. However, despite the quantitative variations, each strain had a similar pattern of enzyme accumulation in fermentation of both substrates. Laccase and MnP activities were high during substrate colonization and declined rapidly during fruiting body development. On the contrary, in substrate colonization P. ostreatus expressed comparatively low activity of hydrolases. When primordia appeared, the activity of t... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1578322 Sun, 01 Jun 2008 04:00:00 +0100 1578322 http://www.medworm.com/index.php?rid=1571839&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18507148%26dopt%3DAbstract Authors: Székely E, Lorinczi L, Bilca D, Fodor E, Sóki J, Sabau M Our aim was to estimate the frequency and characteristics ofmethicillin-resistant Staphylococcus aureus (MRSA) strains occurring in a Romanian teaching hospital. We retrospectively studied isolates from infected or colonized patients treated at the intensive care and surgical units during January 2004-December 2005. The antibiotic susceptibility of MRSA strains and the presence of mecA gene were determined. Consecutively occurring strains isolated through a three-month period were typed using pulsed field gel electrophoresis. A total of 423 S. aureus strains were identified, methicillin-resistance was detected in 211 (49.9%) strains. Most of them were multiresistant. One of the MRSA genotypes identified by PFGE... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571839 Sat, 01 Mar 2008 05:00:00 +0100 1571839 http://www.medworm.com/index.php?rid=1571838&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18507149%26dopt%3DAbstract DISCUSSION: These findings suggest that MCS-18 could be a potent, non-toxic antagonist or a down-regulator of TLR signalling pathway. Investigations on further models are needed to establish ifMCS-18 may influence particularly the production of RA-specific auto-antibodies, too. PMID: 18507149 [PubMed - indexed for MEDLINE] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571838 Sat, 01 Mar 2008 05:00:00 +0100 1571838 http://www.medworm.com/index.php?rid=1571837&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18507150%26dopt%3DAbstract Authors: Saxena S, Shukla S, Thakur A, Gupta R The present work is focused on efficient immobilization of polygalacturonase on polyethylene matrix, followed by its application in apple juice clarification. Immobilization of polygalacturonase on activated polyethylene and its use in apple juice clarification was not reported so far. Aspergillus niger Van Tieghem (MTCC 3323) produced polygalacturonase when grown in modified Riviere's medium containing pectin as single carbon source by fed-batch culture. The enzyme was precipitated with ethanol and purified by gel filtration chromatography (Sephacryl S-100) and immobilized onto glutaraldehyde-activated polyethylene. The method is very simple and time saving for enzyme immobilization. Various characteristics of immobilized enzyme such as o... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571837 Sat, 01 Mar 2008 05:00:00 +0100 1571837 http://www.medworm.com/index.php?rid=1571836&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18507151%26dopt%3DAbstract Authors: Szabó Z, Sóczó G, Miszti C, Hermann P, Rozgonyi F Candida inconspicua is an emerging pathogen in immunocompromised patients possessing inherently decreased susceptibility to fluconazole. We determined the MICs and killing activity of fluconazole and amphotericin B against C. inconspicua clinical isolates as well as reference strain C. inconspicua ATCC 16783 for comparison. MICs were determined using the standard broth microdilution method. Killing rates were determined using time-kill methodology at 0.5-16 x MIC fluconazole and amphotericin B concentrations. Fluconazole and amphotericin B MIC values varied between 16-128 mg/l and 0.5-1 mg/l, respectively. In time kill-assays fluconazole showed fungistatic effect at 1-16 x MIC concentrations against all tested st... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571836 Sat, 01 Mar 2008 05:00:00 +0100 1571836 http://www.medworm.com/index.php?rid=1571835&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18507152%26dopt%3DAbstract Authors: Kristóf K, Barcs I, Cziniel M, Ghidán A, Nagy K The third most frequent agent of perinatal bacterial meningitis is Listeria monocytogenes, in Hungary, its occurrence is, however, uncommon. This raises the possibility of diagnostical mistakes. A connatal listeriosis case validated microbiologically referred to in this report calls attention to Listeria, as a rare but relevant pathogen of neonatal infections. If clinical background suggests infection, the pathogenic role of L. monocytogenes should be taken into consideration. The etiological significance of the agent has to be verified by a competent clinical microbiology laboratory, since maternal listeriosis should be treated and the serious connatal manifestations should be prevented. Epidemiology of perinatal infec... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571835 Sat, 01 Mar 2008 05:00:00 +0100 1571835 http://www.medworm.com/index.php?rid=1571847&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18088007%26dopt%3DAbstract CONCLUSION: Our pilot experiments draw attention the to HMGB-1-inducing ability of Mycobacterium bovis. Assessment of the pathophysiological role of this late cytokine in mycobacterial infections demands further in vitro and in vivo examinations. PMID: 18088007 [PubMed - indexed for MEDLINE] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571847 Sat, 01 Dec 2007 05:00:00 +0100 1571847 http://www.medworm.com/index.php?rid=1571846&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18088008%26dopt%3DAbstract Authors: Rusznyák A, Szabó G, Pollák B, Vágány V, Palatinszky M From reed biofilm samples of Kelemen-szék (Kiskunság National Park, KNP) and Nagy-Vadas (Hortobágy National Park, HNP) altogether 260 bacterial isolates were gained after serial dilutions and plating onto different media. Following a primary selection 164 strains were investigated by "traditional" phenotypic tests and clustered by numerical analysis. Fifty-six representative strains were selected to ARDRA and 16S rDNA sequence analysis for identification. Strains were identified as members of genera Agrobacterium, Paracoccus, Halomonas, Pseudomonas, Bacillus, Planococcus and Nesterenkonia. The species diversity was also investigated by a cultivation independent method. A clone librar... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571846 Sat, 01 Dec 2007 05:00:00 +0100 1571846 http://www.medworm.com/index.php?rid=1571845&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18088009%26dopt%3DAbstract Authors: Okigbo RN, Igwe DI Two pathogens were employed, Streptococcus faecalis and Candida albicans for the study of the antimicrobial effects of Piper guineense and Phyllantus amarus using agar-well diffusion and disc-diffusion methods. Phytochemical screening of ethanol, cold and hot water extracts detected the presence of tannins, saponins, flavonoids, terpenoids and phlobatannins. Antimicrobial effect of the plant extracts showed that the organic solvent and aqueous solvents of P. amarus were inhibitory to S. faecalis while the extracts of Phyllantus amarus were not inhibitory to Candida albicans. Agar-well determined Minimum Inhibitory Concentration (MIC) values ranged between 3.125 mg/ml and 6.25 mg/ml while the disc diffusion determined MIC values ranged between 6.25 mg/ml and ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571845 Sat, 01 Dec 2007 05:00:00 +0100 1571845 http://www.medworm.com/index.php?rid=1571844&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18088010%26dopt%3DAbstract In this study, we describe the membrane lipid composition of eight clinical isolates (azole resistant and sensitive strains) of Candida albicans isolated from AIDS/ HIV patients. Interestingly, fluorescence polarization measurements of the clinical isolates displayed enhanced membrane fluidity in fluconazole resistant strains as compared to the sensitive ones. The increase in fluidity was reflected in the change of membrane order, which was considerably decreased (decrease in fluorescence polarization "p" value denotes higher membrane fluidity) in the resistant strains. The ergosterol content in azole susceptible isolates was greater, almost twice as compared to the resistant isolates. However, no significant alteration was observed in phospholipid and fatty acid composition of these isola... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571844 Sat, 01 Dec 2007 05:00:00 +0100 1571844 http://www.medworm.com/index.php?rid=1571843&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18088011%26dopt%3DAbstract Authors: Mukherjee S, Chakraborty R Plasmid content was investigated in hundred copiotrophic Gram-negative river water isolates that exhibited resistance to four or more antibiotics. A total of seventy-seven isolates were found to carry plasmids of varying sizes. These isolates were primarily grouped as Pseudomonads and members of Enterobacteriaceae on the basis of physiological and biochemical tests. Fifty-six isolates that were rifampicin-sensitive and belonged to Enterobacteriaceae family were chosen as donors for the conjugal transfer assay. Eighteen of the isolates successfully transferred conjugable plasmids to the E. coli DH5alpha recipient. Countable multiple antibiotic resistant transconjugants arose readily and conjugal transfer frequency was in the range of 3.75 x 10(-6) to ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571843 Sat, 01 Dec 2007 05:00:00 +0100 1571843 http://www.medworm.com/index.php?rid=1571842&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18088012%26dopt%3DAbstract This study indicates that heat resistance of strain EC1 was enhanced after acid or thermal adaptation. Heat resistance of strain EC2 was, however, enhanced only after thermal adaptation. For both strains no relationship was found between salt adaptation and the ability to resist thermal stress. PMID: 18088012 [PubMed - indexed for MEDLINE] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571842 Sat, 01 Dec 2007 05:00:00 +0100 1571842 http://www.medworm.com/index.php?rid=1571841&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18088013%26dopt%3DAbstract Authors: Amani , Elahwany MD Cloning of the oriT region derived from Lactococcus lactis subsp. lactis into plasmids is expected to convert them into mobilizable plasmids in a suitable genetic background. In some cases, however, the cloning of oriT causes deleterious DNA rearrangements. In the work presented, oriT was cloned into two temperature sensitive plasmids, pJRS290 and pUCB3522. The plasmids were transformed into E. coli and electroporated into L. lactis. Restriction digestion of the plasmids derived from the cloned transformed cells showed fragment sizes different from those expected, indicating that rearrangements had occurred. Furthermore, the inability to successfully transform E. coli with one of the plasmids supports this conclusion. PMID: 18088013 [PubMed - indexed fo... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571841 Sat, 01 Dec 2007 05:00:00 +0100 1571841 http://www.medworm.com/index.php?rid=1571840&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18088014%26dopt%3DAbstract In conclusions, further studies should be conducted regularly to monitor drug resistance in Turkey in order to manage effectively national tuberculosis control efforts. PMID: 18088014 [PubMed - indexed for MEDLINE] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571840 Sat, 01 Dec 2007 05:00:00 +0100 1571840 http://www.medworm.com/index.php?rid=1571863&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17896473%26dopt%3DAbstract Authors: Mishra NN, Prasad T, Sharma N, Payasi A, Prasad R, Gupta DK, Singh R Pathogenic yeasts from the genus Candida can cause serious infection in humans particularly, in immunocompromised patients and are now recognized as major agents of hospital acquired (nosocomial) infections. In the recent years, there has been a marked increase in the incidence of treatment failures in candidiasis patients receiving long-term antifungal therapy, which has posed a serious problem in its successful use in chemotherapy. Candida cells acquire drug resistance (MDR) during the course of the treatment. The mechanisms of resistance to azole antifungal agents have been elucidated in Candida species and can be mainly categorized as (i) changes in the cell wall or plasma membrane, which lead to impaired... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571863 Sat, 01 Sep 2007 04:00:00 +0100 1571863 http://www.medworm.com/index.php?rid=1571862&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17896474%26dopt%3DAbstract Authors: Puri SC, Amna T, Khajuria A, Gupta A, Arora R, Spiteller M, Qazi GN A novel camptothecin-producing endophytic fungus viz., Entrophospora infrequens was isolated from an important Indian medicinal plant Nothapodytes foetida. The present study reports evaluation ofbioactivities of two novel extracts viz., chloroform (CEEI) and methanolic (MEEI) extracts of Entrophospora infrequens with respect to their immunomodulatory potential in vitro and in vivo (in Balb/c mice). The endophyte E. infrequens was found to synthesize camptothecin, which tested positive in CEEI. The immunomodulatory potential of CEEI and MEEI was compared with standard camptothecin (CPT). Doses of the chloroform extract (CEEI) ranging from 12.5-100 mg/kg body weight, significantly (p < 0.05) stimulated the hu... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571862 Sat, 01 Sep 2007 04:00:00 +0100 1571862 http://www.medworm.com/index.php?rid=1571861&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17896475%26dopt%3DAbstract In this study, Pseudomonas aeruginosa AA 112 which was isolated from soil using enrichment technique could utilize the malathion as a sole carbon source and a source of energy. Pseudomonas aeruginosa AA112 was able to grow in MSMPY medium containing 42.75 mg/ml malathion. However, the optimum concentration of malathion which supported the maximum bacterial growth was found to be 22. 8 mg/ml. Malathion was used as an initial source of energy and carbon when it was found without additional carbon sources (in MSM medium) while it was utilized as second source of energy and carbon in a nutrient-supplemented medium (in MSMPY medium). Moreover, lead acetate test indicated that malathion was first attacked at a sulphur site 1-2 hours after the start of incubation. TLC and IR analysis indicated th... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571861 Sat, 01 Sep 2007 04:00:00 +0100 1571861 http://www.medworm.com/index.php?rid=1571860&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17896476%26dopt%3DAbstract Authors: Younes SA, Csire M, Palyi B, Mikala G, Vályi-Nagy I, Cseh I, Benczik M, Jeney C, Takács T, Simon E, Fülöp V, Berencsi G, Fekete G, Visy M Pregnant women were examined following healthy pregnancies at term. Amniotic fluids were sampled before arteficial rupture of membranes using closed vacutainer system. Blood samples were also taken from the pregnants simultaneously. Endotoxin concentrations of amniotic fluids were tested by the semiquantitative Limulus amebocyte lysate. Both amniotic fluids and blood samples were tested for the presence of DNA of lymphotropic human herpesviruses. The DNA of human papillomaviruses were tested only in the amniotic fluid samples. One-third of the amniotic fluids tested were found to contain measurable amounts of endotoxin. L... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571860 Sat, 01 Sep 2007 04:00:00 +0100 1571860 http://www.medworm.com/index.php?rid=1571859&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17896477%26dopt%3DAbstract Authors: Dencs A, Sebestyén A HGV/GBV-C is a mainly parenterally transmitted Flavivirus that causes a persistent infection. So far no disease has been associated with HGV/GBV-C infection, but its beneficial role in co-infection with the human immunodeficiency virus has been shown in many recent studies. The aim of our study was to determine the frequency of ongoing HGV/GBV-C infections among a sociologically unique group of the Hungarian population, who are at great risk for parenterally transmitted diseases. Viral RNA was detected in 75 serum samples by an RT-PCR method specific for the NS5 region. Nine (12%) samples were positive for HGV/GBV-C RNA. All nine PCR products were sequenced and a phylogenetic analysis was performed to identify the genotypes and subtypes of the detecte... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571859 Sat, 01 Sep 2007 04:00:00 +0100 1571859 http://www.medworm.com/index.php?rid=1571858&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17896478%26dopt%3DAbstract Authors: Gul M, Ciragil P, Bulbuloglu E, Aral M, Alkis S, Ezberci F Comamonas testosteroni is an uncommon isolate in the clinical laboratory as a human pathogen. C. testosteroni most commonly emerges in abdominal pathologies especially in perforated appendicitis. In Turkey we report first time a case of bacteremia due to this organism, in a 22-year-old man with perforated acute appendicitis. The organism was shown to be susceptible to routine antibiotics so it was easily eliminated even after having caused a bacteremia. PMID: 17896478 [PubMed - indexed for MEDLINE] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571858 Sat, 01 Sep 2007 04:00:00 +0100 1571858 http://www.medworm.com/index.php?rid=1571857&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899790%26dopt%3DAbstract Authors: Benedek O, Schubert S Horizontal gene transfer (HGT) plays a key role in the evolution of bacterial pathogens. The exchange of genetic material supplies prokaryotes with several fitness traits enhancing their adaptive response to environmental changes. Pathogenicity islands (PAIs) represent an important and in most cases already immobilized subset of the different vehicles for HGT. Encoding several virulence factors PAls represent a major contribution to bacterial pathogenicity. Nonetheless, the transfer mechanisms of PAIs still remain elusive. We summarise the currently available data regarding the major ways of genetic mobilisation with a focus on the transfer of the Yersinia High-Pathogenicity Island (HPI). PMID: 17899790 [PubMed - indexed for MEDLINE] (Source: Acta Mic... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571857 Fri, 01 Jun 2007 04:00:00 +0100 1571857 http://www.medworm.com/index.php?rid=1571856&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899791%26dopt%3DAbstract Authors: Abo-Amer AE Approximately 63 strains of Lactobacillus acidophilus were isolated from Egyptian home-made cheese and examined for production of antagonism. Only eight strains demonstrated inhibitory activity against spoilage microorganisms (i.e. Staphylococcus aureus and Bacillus cereus) and pathogens (i.e. E. coli, Salmonella sp. and Shigella sp.). Lactobacillus acidophilus AA11 produced higher antimicrobial activity with a wide range of inhibition. The agent AA11 was sensitive to proteolytic enzymes and retained full activity after 30 min at 100 degrees C. Activity against sensitive cells was bactericidal but not bacteriolytic. The compound was produced during growth phase and could be extracted from the culture supernatant fluids with n-butanol. 12% SDS-PAGE analysis of 40% a... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571856 Fri, 01 Jun 2007 04:00:00 +0100 1571856 http://www.medworm.com/index.php?rid=1571855&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899792%26dopt%3DAbstract Authors: Gulati HK, Chadha BS, Saini HS Ten different strains of Thermomyces lanuginosus, isolated from composting soils were found to produce phytase when grown on PSM medium. The wild type strain CM was found to produce maximum amount ofphytase (4.33 units/g DW substrate). Culturing T. lanuginosus strain CM on medium containing wheat bran and optimizing other culture conditions (carbon source, media type, nitrogen source, level of nitrogen, temperature, pH, inoculum age, inoculum level and moisture), increased the phytase yield to 13.26 units/g substrate. This culture was further subjected to UV mutagenesis for developing phytase hyperproducing mutants. The mutant (TL-7) showed 2.29-fold increase in phytase activity as compared to the parental strain. Employing Box-Behnken factor fac... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571855 Fri, 01 Jun 2007 04:00:00 +0100 1571855 http://www.medworm.com/index.php?rid=1571854&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899793%26dopt%3DAbstract Authors: Al-Shaikh SA, Senok AC, Ismaeel AY, Botta GA The association between putative virulence genes in Campylobacter jejuni clinical isolates, in vitro invasive capability and severity of infection is yet to be clearly described. We have characterized three virulence genes and correlated their presence with the severity of infection and in vitro invasiveness. We studied eight C. jejuni strains isolated from patients whose clinical data were scored to determine severity of infection. Cytolethal distending toxin (cdtB), invasion associated marker (iam) and Campylobacter invasion antigen (ciaB) genes were detected by PCR and INT407 cells used for invasion assays. Two strains positive for all three genes were the most invasive and isolated from patients with the most severe infection. F... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571854 Fri, 01 Jun 2007 04:00:00 +0100 1571854 http://www.medworm.com/index.php?rid=1571853&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899794%26dopt%3DAbstract We report an investigation on a collection of multidrug-resistant Enterobacter cloacae isolates from a Hungarian neonatal intensive care unit. All the isolates (n=142) were examined by antimicrobial susceptibility testing and ERIC-PCR. The seven ESBL positive isolates (derived from six patients) made up a separated group with regard to their patterns by antimicrobial susceptibility testing and ERIC-PCR and were further tested by class-1-integron PCR and plasmid electrophoresis. The ESBL isolates were found indistinguishable in each of these laboratory tests, one genetic clone were revealed in the background of ESBL cases by PFGE. The ESBL positive isolates were proven to harbour a approximately 62 Md plasmid and two class-1 integrons (0.9 kb, 1.875 kb). With respect to their clinical relat... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571853 Fri, 01 Jun 2007 04:00:00 +0100 1571853 http://www.medworm.com/index.php?rid=1571852&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899795%26dopt%3DAbstract Authors: Banerjee D, Mondal KC, Pati BR Tannase an industrially important enzyme was produced by Aspergillus aculeatus DBF9 through a solid-state fermentation (SSF). The organism produced good amount of enzyme and gallic acid in wheat bran among the solid substrate used in SSF. Maximum enzyme and gallic acid production occurred in 5% tannic acid after 72 h. Eighty percent initial substrate moisture and 30 degrees C temperature was found suitable for tannase production. PMID: 17899795 [PubMed - indexed for MEDLINE] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571852 Fri, 01 Jun 2007 04:00:00 +0100 1571852 http://www.medworm.com/index.php?rid=1571851&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899796%26dopt%3DAbstract Authors: Vendan RT, Thangaraju M Azospirillum bioinoculant is well known for its high nitrogen fixing and plant growth promoting characters. The carrier based bioinoculants generally suffer from shorter shelf life, poor quality, high contamination and low field performance. Therefore, it is necessary to develop alternative new formulation of inoculants where cyst based inoculants can play significant role. The cyst based liquid formulation was developed by inoculating Azospirillum into the cyst inducing minimal salts medium (MSM). One hundred per cent conversion of vegetative cells into cyst cells was noticed in 96 h. The survival of cyst cells in the MSM was observed up to one year and two months and interestingly, the population level of 10(8) was maintained till the final observatio... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571851 Fri, 01 Jun 2007 04:00:00 +0100 1571851 http://www.medworm.com/index.php?rid=1571850&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899797%26dopt%3DAbstract Authors: Horváth KM, Seregély Z, Dalmadi I, Andrássy E, Farkas J The utility of chemosensor array (EN) signals of head-space volatiles of aerobically stored pork cutlets as a non-invasive technique for monitoring their microbiological load was studied during storage at 4, 8 and 12 degrees C, respectively. The bacteriological quality of the meat samples was determined by standard total aerobic plate counts (TAPC) and colony count of selectively estimated Pseudomonas (PS) spp., the predominant aerobic spoilage bacteria. Statistical analysis of the electronic nose measurements were principal component analysis (PCA), and canonical discriminant analysis (CDA). Partial least squares (PLS) regression was used to model correlation between microbial loads and EN signal responses... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571850 Fri, 01 Jun 2007 04:00:00 +0100 1571850 http://www.medworm.com/index.php?rid=1571849&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17899798%26dopt%3DAbstract Authors: Stepanović S, Vuković D, Djukić S, Cirković I, Svabić-Vlahović M In order to gain insight into the frequency of L. monocytogenes vaginal carriage among women of reproductive age in Belgrade, Serbia, we conducted the long-term investigation (January 1992 through August 2006) described herein. The study population of 958 women included 799 patients with spontaneous abortion and 159 patients with infertility. A low rate of L. monocytogenes vaginal carriage of 0.1% was established, since only one strain was recovered from vaginal swab of a woman with spontaneous abortion. PMID: 17899798 [PubMed - indexed for MEDLINE] (Source: Acta Microbiologica et Immunologica Hungarica) Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571849 Fri, 01 Jun 2007 04:00:00 +0100 1571849 http://www.medworm.com/index.php?rid=1571870&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17523388%26dopt%3DAbstract Authors: Budai I Chlamydia trachomatis an obligate intracellular, Gram-negative bacterium is the causative agent of several acute or chronic, local and systemic human diseases such as trachoma, oculogenital and neonatal infections. It was discovered in 1907 by Halberstaedter and von Prowazek who observed it in conjunctival scrapings from an experimentally infected orangutan. In the last hundred years the detection and study of the intracellular pathogens, including chlamydiae, passed through an enormous evolution. This memorial review is dedicated to these important research and diagnostic discoveries and to the scientists who significantly contributed to this evolution starting from the application of simple light microscopy through the cell culture technique, antibiotic susceptibilit... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571870 Thu, 01 Mar 2007 05:00:00 +0100 1571870 http://www.medworm.com/index.php?rid=1571869&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17523389%26dopt%3DAbstract Authors: Szekeres A, Hatvani L, Leitgeb B A total of 36 UV-induced mutants with altered colony morphology were isolated from strain Trichoderma harzianum T334, a potential biocontrol agent against plant pathogenic fungi with the ability to produce constitutively low levels of chitinases. The level of constitutive beta-1,4-N-acetyl-glucosaminidase production in standing and shaken cultures under non-inductive conditions was tested in mutants and compared to that of the parental strain. About 30% of the mutants showed significantly increased levels of enzyme production, with strain T334 col26a being the best producer. This mutant and the parental strain were subjected to in vitro confrontation assays with plant pathogenic Fusarium culmorum, Pythium debaryanum and Rhizoctonia solani strai... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571869 Thu, 01 Mar 2007 05:00:00 +0100 1571869 http://www.medworm.com/index.php?rid=1571868&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17523390%26dopt%3DAbstract Authors: Geleta A, Kristóf Z, Maráz A Scanning and transmission electron microscopic studies revealed the presence of slime-like, amorphous material on the surface of Schizosaccahromyces pombe RIVE 4-2-1 cells, independently, whether they were in flocculated or in non-flocculated state. Close contact of the adjacent cells via the merging outermost cell wall layers was found, however, only in the case of floc formation, which was induced by cultivating the cells in the presence of 6% (v/v) ethanol. Irreversible loss of the flocculation ability of the cells by treatment with proteinases suggests that proteinaceous cell surface molecules as lectins contribute to the cell-to-cell interaction during flocculation. Both proteinase K and pronase treatments removed a distinct outer la... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571868 Thu, 01 Mar 2007 05:00:00 +0100 1571868 http://www.medworm.com/index.php?rid=1571867&cid=s_37348_77_f&fid=37348&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17523391%26dopt%3DAbstract Authors: Tauber T, Berta B, Székely AJ, Gyarmati I, Kékesi K, Márialigeti K, Tóth EM The aim of the present work was to compare the microbial communities of a mesophilic and a thermophilic pilot scale anaerobe sludge digester. For studying the communities cultivation independent chemotaxonomical methods (RQ and PLFA analyses) and T-RFLP were applied. Microbial communities of the mesophilic and thermophilic pilot digesters showed considerable differences, both concerning the species present, and their abundance. A Methanosarcina sp. dominated the thermophilic, while a Methanosaeta sp. the mesophilic digester among Archaea. Species diversity of Bacteria was reduced in the thermophilic digester. Based on the quinone patterns in both digesters the dominance of sulphate ... Acta Microbiologica et Immunologica Hungarica journals http://www.medworm.com/rss/comments.php?id=1571867 Thu, 01 Mar 2007 05:00:00 +0100 1571867