MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'American Journal of Clinical Pathology' source. Sat, 20 Mar 2010 14:59:27 +0100 http://www.medworm.com/index.php?rid=3376881&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231603%26dopt%3DAbstract Authors: Wilson ML PMID: 20231603 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376881 Thu, 18 Mar 2010 15:41:17 +0100 3376881 http://www.medworm.com/index.php?rid=3376880&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231604%26dopt%3DAbstract Authors: Upton MP PMID: 20231604 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376880 Thu, 18 Mar 2010 15:41:14 +0100 3376880 http://www.medworm.com/index.php?rid=3376878&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231605%26dopt%3DAbstract Authors: Smith BR, Aguero-Rosenfeld M, Anastasi J, Baron B, Berg A, Bock JL, Campbell S, Crookston KP, Fitzgerald R, Fung M, Haspel R, Howe JG, Jhang J, Kamoun M, Koethe S, Krasowski MD, Landry ML, Marques MB, Rinder HM, Roberts W, Schreiber WE, Spitalnik SL, Tormey CA, Wolf P, Wu YY, As the 100th anniversary of the Flexner report nears, medical student education is being reviewed at many levels. One area of concern, expressed in recent reports from some national health care organizations, is the adequacy of training in the discipline of laboratory medicine (also termed clinical pathology). The Academy of Clinical Laboratory Physicians and Scientists appointed an ad hoc committee to review this topic and to develop a suggested curriculum, which was subsequently forwarded to the entire... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376878 Thu, 18 Mar 2010 15:41:11 +0100 3376878 http://www.medworm.com/index.php?rid=3376862&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231606%26dopt%3DAbstract Authors: George E The association of prolonged microscope use with the development of chronic pain syndromes has been recognized for nearly 3 decades; yet most pathologists are not well-informed about this hazard until after they develop a problem. The purpose of this article is to make pathologists aware of this risk, discuss current pathogenetic models, and encourage them to proactively integrate prevention strategies into their daily lives. PMID: 20231606 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376862 Thu, 18 Mar 2010 15:41:08 +0100 3376862 http://www.medworm.com/index.php?rid=3376856&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231607%26dopt%3DAbstract Authors: Amukele TK, Ferrell C, Chandler WL We compared plasma with whole blood (WB) international normalized ratio (INR) and fibrinogen using the same instrument and reagents. WBINRs were 50% higher than plasma INRs. After increasing the WB sample volume 40% and adjusting the International Sensitivity Index, WBINRs were similar to plasma INRs [adjusted WBINR = 0.99(plasma INR) - 0.02; r(2) = 0.98; n = 155], but the average difference in WB vs plasma INR was 4-fold higher than duplicate plasma INRs. Variation in hematocrit was a major determinant of the accuracy of the WBINR, with increased error at high INRs. The WB fibrinogen assay was highly dependent on the sample hematocrit (r(2) = 0.83), even after the sample volume was adjusted. Accurate WB fibrinogen measurements required a mat... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376856 Thu, 18 Mar 2010 15:41:03 +0100 3376856 http://www.medworm.com/index.php?rid=3376838&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231608%26dopt%3DAbstract This study demonstrates the feasibility of using 2 different extraction techniques to yield results within 0.5 log(10) copies/mL of the mean value, a level that would allow for clinical comparison between different laboratory assays. PMID: 20231608 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376838 Thu, 18 Mar 2010 15:40:56 +0100 3376838 http://www.medworm.com/index.php?rid=3376837&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231609%26dopt%3DAbstract Authors: Yazer MH, Stapor D, Triulzi DJ We compare our experience using a new rapid qualitative immunoassay (RQI) test (platelet Pan Genera Detection, Verax, Worcester, MA) for bacterial screening of whole blood platelet (WBP) pools with our previous WBP bacterial screen, pH testing. All WBP pools were RQI tested at the time of issue. All RQI+ pools were cultured in an automated culture system, with subsequent bacterial identification if the culture was positive. During approximately 5.5 months, 7,733 WBP pools were RQI tested. There were 14 positive RQI tests; 12 WBP pools were sterile when cultured and considered false-positive RQI tests. One pool was positive for coagulase-negative Staphylococcus, while another was positive for group B Streptococcus. The specificity and positive pre... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376837 Thu, 18 Mar 2010 15:40:53 +0100 3376837 http://www.medworm.com/index.php?rid=3376836&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231610%26dopt%3DAbstract Authors: Lee SH, Vigliotti VS, Vigliotti JS, Jones W, Pappu S The DNA of Borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (PCR) amplification of the species-specific 16S ribosomal DNA (rDNA). The primers were those well known to be specific for signature sequence amplification of the B burgdorferi sensu lato 16S ribosomal RNA gene. The positive 293-base-pair nested PCR amplicon was subjected to routine direct automated Sanger sequencing. A 50-base sequence excised randomly from the sequencing electrophoretogram between the 2 nested PCR primer binding sites was sufficient for the Basic Local Alignment Search Tool (BLAST) analysis to validate the B burgdorferi sensu lato 16S rDNA without a reasonable doubt. Nes... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376836 Thu, 18 Mar 2010 15:40:44 +0100 3376836 http://www.medworm.com/index.php?rid=3376835&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231611%26dopt%3DAbstract Authors: Wang J, Zhang Y, Xu D, Shao W, Lu Y Diagnosis of urinary tract infection (UTI) is primarily done by microbiologic culture, which is time-consuming and can produce false-positives and false-negatives. Flow cytometry allows for rapid screening of many samples and eliminates culturing. We analyzed the Sysmex UF-1000i (TOA Medical Electronics, Kobe, Japan) for accuracy in identifying RBCs and WBCs, casts, bacteria, and epithelia. We also evaluated its precision, linear estimation of results, carryover contamination rate, and anti-interference. UF-1000i agreement with manual counting was approximately 95% for RBCs and WBCs, epithelia, and casts. Its coefficient of variation for bacteria ranged from 4.7% to 15.2%. UF-1000i screening for UTIs exhibited great sensitivity (97%), specif... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376835 Thu, 18 Mar 2010 15:40:41 +0100 3376835 http://www.medworm.com/index.php?rid=3376834&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231612%26dopt%3DAbstract We present a case of a spleen revealing histologic features of follicular lymphoma, without any indication of a second lymphoma. Immunohistochemical stains supported this diagnosis and showed the follicular lymphoma to be BCL2-. However, these studies revealed 2 additional unexpected findings: cyclin D1+ mantle zone cells surrounding neoplastic and reactive follicles (indicative of in situ mantle cell lymphoma) and BCL2-bright, histologically nonneoplastic follicles (indicative of in situ follicular lymphoma). ImmunoFISH and microdissection and polymerase chain reaction analysis documented the clonal nature of the cyclin D1+ mantle zones and illustrated clonal independence from the follicular lymphoma. This case illustrates an uncommon and unusual composite follicular and mantle cell lymph... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376834 Thu, 18 Mar 2010 15:40:32 +0100 3376834 http://www.medworm.com/index.php?rid=3376833&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231613%26dopt%3DAbstract Authors: Shao H, Yuan CM, Xi L, Raffeld M, Morris JC, Janik JE, Stetler-Stevenson M Little information exists regarding the detection of minimal residual disease (MRD) in adult T-cell leukemia/lymphoma (ATLL). We evaluated 75 peripheral blood samples from 17 ATLL cases using flow cytometry (FC); 50 of the samples were concurrently evaluated by polymerase chain reaction (PCR) for clonal T-cell receptor gamma chain (TRG) gene rearrangement and the presence of human T-cell lymphotropic virus-1 proviral sequences. Residual ATLL cells were identified using a multiparametric approach to identify aberrant T-cell immunophenotypes. Malignant T cells were CD4+, CD3 dim+, CD26-, CD25 bright, CD7+, and CD27+, with occasional dim expression of CD2 or CD5. FC exhibited a high sensitivity, detecting ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376833 Thu, 18 Mar 2010 15:40:28 +0100 3376833 http://www.medworm.com/index.php?rid=3376832&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231614%26dopt%3DAbstract Authors: Klco JM, Vij R, Kreisel FH, Hassan A, Frater JL Myeloproliferative neoplasms (MPNs; formerly chronic myeloproliferative disorders) are a class of myeloid hematologic malignancies that represent a stem cell-derived expansion of 1 or more hematopoietic cell lineages. The current 2008 World Health Organization system recognizes 8 types of MPN: chronic myelogenous leukemia, chronic neutrophilic leukemia, polycythemia vera, primary myelofibrosis, essential thrombocythemia, chronic eosinophilic leukemia, mastocytosis, and myeloproliferative neoplasm, unclassifiable. This review summarizes the salient characteristics of the MPNs, with emphasis on recent developments in the molecular pathophysiology and therapeutic monitoring of these disorders. PMID: 20231614 [PubMed - in process... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376832 Thu, 18 Mar 2010 15:40:24 +0100 3376832 http://www.medworm.com/index.php?rid=3376831&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231615%26dopt%3DAbstract We describe 10 cases of primary well-differentiated neuroendocrine carcinomas (carcinoid tumor) of the lung with extensive sclerotic changes. The patients were 6 women and 4 men from 20 to 69 years of age. Clinically, patients had symptoms of bronchial obstruction such as cough, dyspnea, and chest pain. Surgical resection of the tumors was accomplished in all the cases. Histologically, all tumors corresponded to the well-differentiated type; however, in 4 cases, lymph node metastases were present. Immunohistochemically, all tumors showed positive staining for neuroendocrine markers, including chromogranin, synaptophysin, CD56, and broad-spectrum keratin. Follow-up information showed that 8 patients were alive after a period ranging from 1 to 5 years. The cases presented highlight an import... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376831 Thu, 18 Mar 2010 15:40:21 +0100 3376831 http://www.medworm.com/index.php?rid=3376830&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231616%26dopt%3DAbstract Authors: Bellizzi AM, Rock J, Marsh WL, Frankel WL We performed a histologic and immunohistochemical assessment of 53 noninvasive appendiceal epithelial proliferations, appropriating terminology and using markers shown useful in differentiating serrated colorectal polyps. These were classified as hyperplastic polyp (HP), sessile serrated adenoma (SSA), mixed serrated and adenomatous lesion (MSAL), mucinous cystadenoma (MCA), or conventional adenoma (CAD). Immunohistochemical analysis for cytokeratin (CK) 20, Ki-67, MUC6, and beta-catenin was performed. Diagnoses were as follows: HP, 6; SSA, 12; HP vs SSA, 3; MSAL, 16; MCA, 14; and CAD, 2. All HPs showed expanded (beyond surface) CK20 and expanded or normal (base) Ki-67; 1 was MUC6+. Most SSAs and MSALs were CK20-expanded or expanded wi... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376830 Thu, 18 Mar 2010 15:40:17 +0100 3376830 http://www.medworm.com/index.php?rid=3376829&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231617%26dopt%3DAbstract Authors: Gautam U, Srinivasan R, Rajwanshi A, Bansal D, Marwaha RK, Vasishtha RK We evaluated the feasibility and usefulness of reverse transcriptase-polymerase chain reaction (RT-PCR) on fine-needle aspirates for categorization of small blue round cell tumors (SBRCTs). A total of 51 cases, including 25 Ewing sarcoma/peripheral primitive neuroectodermal tumors (PNETs), 11 rhabdomyosarcomas, 13 neuroblastomas, and 2 desmoplastic small round cell tumors (DSRCTs) were analyzed. The detection of the EWS-FLI1 (20/25) and EWS-ERG (4/25) fusion transcripts resolved 24 of 25 cases of Ewing sarcoma/PNET. The PAX3/7-FKHR fusion transcript was detected in 2 of 4 cases of alveolar rhabdomyosarcoma and the EWS-WT1 transcript in both cases of DSRCT. Tyrosine hydroxylase and 3,4-dihydroxyphenylalanin... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376829 Thu, 18 Mar 2010 15:40:14 +0100 3376829 http://www.medworm.com/index.php?rid=3376828&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231618%26dopt%3DAbstract This study confirmed the importance of tumor ulceration and the number of positive lymph nodes on outcome. PMID: 20231618 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376828 Thu, 18 Mar 2010 15:40:11 +0100 3376828 http://www.medworm.com/index.php?rid=3376827&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231619%26dopt%3DAbstract Authors: O'Brien C, True LD, Higano CS, Rademacher BL, Garzotto M, Beer TM Clinical trials are evaluating the effect of neoadjuvant chemotherapy on men with high-risk prostate cancer. Little is known about the clinical significance of postchemotherapy tumor histopathologic features. We assessed the prognostic and predictive value of histologic features (intraductal carcinoma, vacuolated cell morphologic features, inconspicuous glands, cribriform architecture, and inconspicuous cancer cells) observed in 50 high-risk prostate cancers treated with preprostatectomy docetaxel and mitoxantrone. At a median follow-up of 65 months, the overall relapse-free survival (RFS) rates at 2 and 5 years were 65% and 49%, respectively. In univariate analyses (using the Kaplan-Meier method and log-rank te... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376827 Thu, 18 Mar 2010 15:40:08 +0100 3376827 http://www.medworm.com/index.php?rid=3376826&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20231620%26dopt%3DAbstract Authors: Davidson DD, Koch MO, Lin H, Jones TD, Biermann K, Cheng L Previous studies suggest that low prostate weight is a significant negative prognostic factor for prostate cancer. In the current study, the data for 431 men who underwent radical retropubic prostatectomy between 1990 and 1998 were analyzed for association between prostate weight and various clinical and pathologic parameters. These included age, preoperative prostate-specific antigen (PSA) level, PSA recurrence, pathologic stage, Gleason grade, extraprostatic extension, positive surgical margins, tumor volume, associated high-grade prostatic intraepithelial neoplasia, perineural invasion, and lymph node metastasis. Potential associations were probed by using Cox regression model analysis. A significant positive correl... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3376826 Thu, 18 Mar 2010 15:40:06 +0100 3376826 http://www.medworm.com/index.php?rid=3275674&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154272%26dopt%3DAbstract Authors: Cin PD, Aster JC, Deangelo DJ PMID: 20154272 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275674 Tue, 16 Feb 2010 16:43:14 +0100 3275674 http://www.medworm.com/index.php?rid=3275673&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154273%26dopt%3DAbstract Authors: Torlakovic EE, Riddell R, Banerjee D, El-Zimaity H, Pilavdzic D, Dawe P, Magliocco A, Barnes P, Berendt R, Cook D, Gilks B, Williams G, Perez-Ordonez B, Wehrli B, Swanson PE, Otis CN, Nielsen S, Vyberg M, Butany J Immunohistochemical and immunocytochemical assays are highly complex diagnostic analyses used to aid in the accurate identification and biologic characterization of tissue types in neoplastic and nonneoplastic diseases. Immunohistochemical tests are applied mainly to the diagnosis of neoplasms. Some immunohistochemical tests provide information of important prognostic and predictive value in selected human neoplasms and, as such, are often critical for the appropriate and effective treatment of patients. This document provides recommendations and opinions of the Cana... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275673 Tue, 16 Feb 2010 16:43:10 +0100 3275673 http://www.medworm.com/index.php?rid=3275672&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154274%26dopt%3DAbstract Authors: Marchevsky AM, Wick MR PMID: 20154274 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275672 Tue, 16 Feb 2010 16:43:07 +0100 3275672 http://www.medworm.com/index.php?rid=3275671&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154275%26dopt%3DAbstract Authors: George E, Polissar NL, Wick M We evaluated the usefulness of immunohistochemical examination for E-cadherin, p16, and cyclin D1 in discriminating melanoma from Spitz tumors. Immunoperoxidase staining was performed on formalin-fixed tissue specimens from 46 Spitz tumors and 42 concurrent melanoma specimens. The percentages of immunoreactive melanocytes in the epidermis and dermis were estimated semiquantitatively. Qualitatively abnormal immunoreactivity patterns were also tabulated. Dermal p16 immunoreactivity was the best quantitative discriminator: decreased nuclear immunoreactivity (<25% of dermal melanocytes) was 3-fold more likely in melanoma than in Spitz tumors (P = .004). Loss of both nuclear and cytoplasmic dermal p16 immunoreactivity was 8-fold more likely in melan... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275671 Tue, 16 Feb 2010 16:43:04 +0100 3275671 http://www.medworm.com/index.php?rid=3275670&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154276%26dopt%3DAbstract Authors: Mukhopadhyay S, Philip AT, Stoppacher R Although novel influenza A (H1N1) virus infection has assumed pandemic proportions, there are few reports of the pathologic findings. Herein we describe the pathologic findings of novel influenza A (H1N1) infection based on findings in 2 autopsy cases. The first patient, a 36-year-old man, had flu-like symptoms; oseltamivir (Tamiflu) therapy was started 8 days after onset of symptoms, and he died on day 15 of his illness. At autopsy, the main finding was diffuse alveolar damage with extensive fresh intra-alveolar hemorrhage. The second patient, a 46-year-old woman with alcoholism, was found unresponsive in a basement and brought to the hospital intoxicated and confused. Her condition deteriorated rapidly, and she died 4 days after admiss... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275670 Tue, 16 Feb 2010 16:43:00 +0100 3275670 http://www.medworm.com/index.php?rid=3275669&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154277%26dopt%3DAbstract Authors: Wünsch K, Müller J, Jähnig H, Herrmann RA, Arnholdt HM, Märkl B Pericolonic tumor deposits (PTDs) are associated with an adverse outcome in colorectal cancer. According to the International Union Against Cancer they are classified as N1 or V1/V2 depending on their shape. This recommendation, however, is not well supported by the literature. To elucidate the origin of PTDs, we performed a histomorphologic study of 69 PTDs, which were found in 7 of 21 colorectal specimens using the whole-mount step-section technique. Depending on the origin, the nodules were classified as venous invasions, lymphatic invasions, nerve sheath infiltrations, free PTDs, and continuous growth in 18 (26%), 3 (4%), 6 (9%), 34 (49%), and 8 (12%) of 69 PTDs, respectively. Polycyclic an... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275669 Tue, 16 Feb 2010 16:42:57 +0100 3275669 http://www.medworm.com/index.php?rid=3275668&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154278%26dopt%3DAbstract Authors: Bergeron C, Ordi J, Schmidt D, Trunk MJ, Keller T, Ridder R, The histopathologic interpretation of cervical intraepithelial neoplasia (CIN) is subject to a high level of interobserver variability and a substantial number of false-positive and false-negative results. We assessed the impact of the conjunctive interpretation of p16(INK4a)-immunostained slides on the accuracy of community-based pathologists in diagnosing high-grade cervical intraepithelial neoplasia (CIN; CIN 2 and CIN 3) in biopsy specimens. Twelve pathologists rendered independent diagnoses on a set of 500 H&E-stained cervical punch and conization specimens. Results were compared with a dichotomized "gold standard" established by consensus of 3 gynecopathology experts. When p16(INK4a)-immunostained slides w... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275668 Tue, 16 Feb 2010 16:42:55 +0100 3275668 http://www.medworm.com/index.php?rid=3275667&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154279%26dopt%3DAbstract Authors: Morgenstern DA, Hasan F, Gibson S, Winyard P, Sebire NJ, Anderson J The Pax gene family encodes transcription factors with similar structures but distinctive roles in development and with limited expression in adult tissues. Reexpression of PAX proteins is frequently observed in human cancers, reflecting recapitulation of embryologic or developmental function. Defining expression of PAX family members is important in the immunohistochemical differential diagnosis of cancer, understanding oncogenesis, and defining targets for therapy. Immunostaining for PAX5 has become a commonly used technique in differential diagnosis of B-lineage hematologic malignancies. In seeking to define the range and degree of expression of PAX5 in nonhematologic pediatric cancers by immunohistochemica... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275667 Tue, 16 Feb 2010 16:42:52 +0100 3275667 http://www.medworm.com/index.php?rid=3275666&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154280%26dopt%3DAbstract Authors: Idirisinghe PK, Thike AA, Cheok PY, Tse GM, Lui PC, Fook-Chong S, Wong NS, Tan PH Estrogen receptor (ER), progesterone receptor (PR), and c-ERBB2 (HER2/neu) are therapeutically and prognostically important markers in the management of breast carcinoma. They are not always analyzed in distant metastatic and locally recurrent breast cancers. We compared immunohistochemical expression in a series of primary breast carcinomas with their distant metastases (n = 72) and local recurrences (n = 45) and analyzed the impact of any changes on survival. Discordance rates between primary and metastatic and between primary and locally recurrent lesions, respectively, were 18% (13/72) and 13% (6/45) for ER, 42% (30/72) and 33% (15/45) for PR, and 7% (5/72) and 2% (1/45) for c-ERBB2. There wa... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275666 Tue, 16 Feb 2010 16:42:49 +0100 3275666 http://www.medworm.com/index.php?rid=3275653&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154281%26dopt%3DAbstract Authors: Lee H, Stapp RT, Ormsby AH, Shah VV Autoimmune hepatitis (AIH)-primary biliary cirrhosis (PBC) overlap syndrome (OS) is a vaguely defined entity demonstrating features of AIH and PBC. We investigated the usefulness of IgG and IgM immunostaining for the distinction of AIH and PBC and their staining pattern in cases of possible OS. The approximate quantity of IgG+ and IgM+ periportal inflammatory cells in immunohistochemical analysis were compared in cases of AIH, PBC, and OS. AIH cases showed predominant IgG immunostaining of periportal inflammatory cells. A significant number of PBC cases also demonstrated IgG predominance rather than IgM. Six OS cases had IgG predominance, 4 had IgM predominance, and 1 was equivocal. The usefulness of IgG and IgM immunostaining is limited in ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275653 Tue, 16 Feb 2010 16:42:46 +0100 3275653 http://www.medworm.com/index.php?rid=3275652&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154282%26dopt%3DAbstract This report uses 2 case studies to illustrate some of the unique challenges facing laboratory managers confronted with a large-scale testing error. We identify 9 distinct constituencies that may be impacted by large-scale testing errors, each of which requires laboratory management's thoughtful and timely attention. PMID: 20154282 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275652 Tue, 16 Feb 2010 16:42:43 +0100 3275652 http://www.medworm.com/index.php?rid=3275651&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154283%26dopt%3DAbstract Authors: Olson KN, Luckenbill K, Thompson J, Middleton O, Geiselhart R, Mills KM, Kloss J, Apple FS Fentanyl concentrations were measured in postmortem specimens collected in 20 medical examiner cases from femoral blood (FB), heart blood (HB), heart tissue, liver tissue, and skeletal muscle. Unique was a subset of 7 cases in which FB was obtained at 2 postmortem intervals, shortly after death (FB1) and at autopsy (FB2). The mean collection times of FB1 and FB2 after death were 4.0 and 21.6 hours, respectively. Fentanyl concentrations for FB1 and FB2 ranged from undetectable to 14.6 mug/L (mean, 4.6 mug/L) and 2.0 to 52.5 mug/L (mean, 17.3 mug/L), respectively. Corresponding mean HB, liver tissue, and heart tissue fentanyl concentrations were 29.8 mug/L, 109.7 mg/kg, and 103.4 mg/kg, re... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275651 Tue, 16 Feb 2010 16:42:40 +0100 3275651 http://www.medworm.com/index.php?rid=3275650&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154284%26dopt%3DAbstract Authors: Sobhani K, Garrett DA, Liu DP, Rainey PM Leflunomide (Arava), a drug with immunosuppressive and antiviral effects, is being used in renal transplant recipients, primarily for its action against BK polyomavirus (BKV), which affects 1% to 10% of renal transplant recipients and often causes failure of grafted kidneys. Leflunomide effects are solely due to an active metabolite, teriflunomide (formerly A77 1726). Trough blood concentrations of teriflunomide exceeding 40 mug/mL (148 mumol/L) are associated with progressive clearance of BKV. Toxic effects become increasingly apparent at higher concentrations. We have developed a rapid, simple, and robust high-performance liquid chromatography (HPLC) method for therapeutic monitoring of teriflunomide in renal transplant recipients. Sa... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275650 Tue, 16 Feb 2010 16:42:37 +0100 3275650 http://www.medworm.com/index.php?rid=3275649&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154285%26dopt%3DAbstract Authors: Park YA, Waldrum MR, Marques MB Thrombotic thrombocytopenic purpura-hemolytic uremic syndrome (TTP-HUS) and disseminated intravascular coagulation (DIC) may have identical manifestations in adults. Because TTP-HUS is 90% fatal without plasma exchange, prompt diagnosis is essential. To test the hypothesis that routine laboratory assays can discriminate between the 2 entities, we retrospectively identified adult patients with TTP-HUS and matched each with 2 patients with DIC. Although the platelet count, prothrombin time (PT), and partial thromboplastin time were different (P < .05) between the 2 patient groups, after regression analysis, only PT and profound thrombocytopenia remained associated with TTP-HUS (P = .001 and P = .003, respectively). A platelet count of less than... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275649 Tue, 16 Feb 2010 16:42:31 +0100 3275649 http://www.medworm.com/index.php?rid=3275648&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154286%26dopt%3DAbstract Authors: Czuchlewski DR, Brackney M, Ewers C, Manna J, Fekrazad MH, Martinez A, Nolte KB, Hjelle B, Rabinowitz I, Curtis BR, McFarland JG, Baumbach J, Foucar K Levamisole is a known contaminant of cocaine and, via this route, has been associated with otherwise unexplained agranulocytosis. Levamisole is currently present in the majority of cocaine samples seized by the US Drug Enforcement Agency. We identified 20 cases of unexplained agranulocytosis in our practice locations of Albuquerque, NM, and Vancouver, Canada. Epidemiologic investigation revealed recent or ongoing cocaine use in 14 cases (70%). Certain morphologic features, including circulating plasmacytoid lymphocytes, increased bone marrow plasma cells, and mild megakaryocytic hyperplasia, were associated with the cocaine-expo... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275648 Tue, 16 Feb 2010 16:42:28 +0100 3275648 http://www.medworm.com/index.php?rid=3275647&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154287%26dopt%3DAbstract Authors: Anderson MW, Zhao S, Ai WZ, Tibshirani R, Levy R, Lossos IS, Natkunam Y Chemokine receptor 1 (CCR1) is a G protein-coupled receptor that binds to members of the C-C chemokine family. Recently, CCL3 (MIP-1alpha), a high-affinity CCR1 ligand, was identified as part of a model that independently predicts survival in patients with diffuse large B-cell lymphoma (DLBCL). However, the role of chemokine signaling in the pathogenesis of human lymphomas is unclear. In normal human hematopoietic tissues, we found CCR1 expression in intraepithelial B cells of human tonsil and granulocytic/monocytic cells in the bone marrow. Immunohistochemical analysis of 944 cases of hematolymphoid neoplasia identified CCR1 expression in a subset of B- and T-cell lymphomas, plasma cell myeloma, acute mye... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275647 Tue, 16 Feb 2010 16:42:26 +0100 3275647 http://www.medworm.com/index.php?rid=3275646&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154288%26dopt%3DAbstract Authors: Dimov ND, Medeiros LJ, Ravandi F, Bueso-Ramos CE Despite the success of the current therapy for patients with acute promyelocytic leukemia (APL), relapse occurs in up to 30% of patients. The characteristics of relapsed APL are not well described. We evaluated a group of APL cases at relapse and compared the clinicopathologic, immunophenotypic, molecular, and cytogenetic findings with those at initial diagnosis. From a group of 207 patients with APL, in 38 patients morphologic evidence of relapse developed. In 30 patients relapse was isolated to bone marrow, and 8 had extramedullary disease. Blasts were morphologically stable in 37 patients. Changes in the immunophenotypic profile were common, the most frequent being gain of CD34, HLA-DR, or CD33 and attenuation or loss of CD13... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275646 Tue, 16 Feb 2010 16:42:20 +0100 3275646 http://www.medworm.com/index.php?rid=3275645&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154289%26dopt%3DAbstract This study was undertaken to evaluate the variable clinical expression of hemoglobin (Hb) H disease in India. For the study, alpha genotyping was done in 8 patients with Hb H disease using multiplex polymerase chain reaction and DNA sequencing. The study revealed that 4 genotypes (- -(SEA)/ -alpha(3.7), - -(SA)/-alpha(3.7), - -(SEA)/-alpha(3.7 Sallanches), - -alpha(3.7)/-alpha(3.7 Sallanches)) were responsible for Hb H disease, the alpha+ thalassemia mutation (-alpha(3.7) deletion) being the most common defect. The nondeletional mutation Hb Sallanches (alpha 2 codon 104 G --> A) was seen in 3 cases. Two unique and novel genotypes leading to Hb H disease were characterized (- -(SEA)/-alpha(3.7 Sallanches) and -alpha(3.7)/-alpha(3.7 Sallanches)). Because a majority of patients with Hb H d... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275645 Tue, 16 Feb 2010 16:42:16 +0100 3275645 http://www.medworm.com/index.php?rid=3275642&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154290%26dopt%3DAbstract Authors: Hoda SA, Besanceney C PMID: 20154290 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275642 Tue, 16 Feb 2010 16:42:12 +0100 3275642 http://www.medworm.com/index.php?rid=3275641&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20154291%26dopt%3DAbstract Authors: Hsu JD, Yao CC, Han LW, Han CP PMID: 20154291 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3275641 Tue, 16 Feb 2010 16:42:05 +0100 3275641 http://www.medworm.com/index.php?rid=3202209&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093225%26dopt%3DAbstract Authors: Horowitz GL PMID: 20093225 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202209 Mon, 25 Jan 2010 06:45:08 +0100 3202209 http://www.medworm.com/index.php?rid=3202208&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093226%26dopt%3DAbstract Authors: Katayev A, Balciza C, Seccombe DW Reference intervals are essential for clinical laboratory test interpretation and patient care. Methods for estimating them are expensive, difficult to perform, often inaccurate, and nonreproducible. A computerized indirect Hoffmann method was studied for accuracy and reproducibility. The study used data collected retrospectively for 5 analytes without exclusions and filtering from a nationwide chain of clinical reference laboratories in the United States. The accuracy was assessed by the comparability of reference intervals as calculated by the new method with published peer-reviewed studies, and reproducibility was assessed by the comparability of 2 sets of reference intervals derived from 2 different data sets. There was no statistically si... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202208 Mon, 25 Jan 2010 06:45:05 +0100 3202208 http://www.medworm.com/index.php?rid=3202207&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093227%26dopt%3DAbstract Authors: Vollmer RT I have examined more than 800 values of serum prostate-specific antigen (PSA) in 119 American Veterans during the time before their diagnosis of prostate cancer. These values appear to follow an exponential model with respect to time. Specifically, the model comprises a sum of 2 exponential functions: one for an early, slowly rising component of PSA and a second for a later, faster rising component. The parameters of each component comprise an amplitude and a relative velocity. Whereas the relative velocity of the slow component is significantly associated with the volume of benign tissue, both the amplitude and relative velocity of the fast component are significantly associated with the volume of tumor. The results suggest that at the time of diagnosis of prostate... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202207 Mon, 25 Jan 2010 06:45:03 +0100 3202207 http://www.medworm.com/index.php?rid=3202206&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093228%26dopt%3DAbstract This study evaluated 66 POC specimens by flow cytometry, digital image analysis, p57 immunohistochemical analysis, and fluorescence in situ hybridization (FISH). The final diagnosis, based on the combined analysis of all test results, included 33 HAs, 24 PMs, and 9 CMs. The p57 immunostain identified 9 CMs that were evaluated as nontriploid by all other techniques. FISH seems to have the best accuracy (100%) for determining whether a specimen contains a triploid chromosome complement. These data suggest that the combination of p57 and FISH seems to be the best ancillary testing strategy to aid pathologists in the appropriate identification of CM, PM, and HA in POC specimens. PMID: 20093228 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202206 Mon, 25 Jan 2010 06:45:00 +0100 3202206 http://www.medworm.com/index.php?rid=3202205&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093229%26dopt%3DAbstract Authors: Hoff K, Jørgensen JT, Müller S, Røngaard E, Rasmussen O, Schønau A The overall purpose of the study was to demonstrate applicability of the DAKO dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup) with respect to 4 fluorescence in situ hybridization (FISH) probes: MYC (c-MYC), EGFR, ERBB2 (HER2), and TOP2A. The study showed that the dual-color CISH assay can convert Texas red and fluorescein isothiocyanate (FITC) signals into chromogenic signals with an almost complete 1:1 conversion ratio. Agreement studies between the FISH assays for HER2 and TOP2A and the corresponding CISH conversion assays showed 100% concordance (kappa values of 1.0) between the CISH and FISH methods for HER2 and TOP2A status. The correlations of the gen... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202205 Mon, 25 Jan 2010 06:44:57 +0100 3202205 http://www.medworm.com/index.php?rid=3202204&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093230%26dopt%3DAbstract Authors: Sasaki M, Ikeda H, Yamaguchi J, Miyakoshi M, Sato Y, Nakanuma Y We investigated the pathologic significance of ductular reactions in chronic liver diseases with respect to cellular senescence. The expression of senescence-associated markers (p16(INK4a) and p21(WAF1/Cip1)), cell proliferation, cell cycle markers (cyclin D and cyclin A), and neural cell adhesion molecule (NCAM) was examined immunohistochemically in primary biliary cirrhosis (PBC, n = 37), chronic viral hepatitis (n = 39), nonalcoholic steatohepatitis (n = 25), and control normal livers (n = 12). The expression of p16(INK4a) and p21(WAF1/Cip1) was frequently found in ductular cells in the advanced stage of chronic liver diseases, especially in PBC (P < .05). Double immunostaining disclosed that most senescent ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202204 Mon, 25 Jan 2010 06:44:53 +0100 3202204 http://www.medworm.com/index.php?rid=3202203&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093231%26dopt%3DAbstract Authors: Jakowski JD, Mayerson J, Wakely PE We reviewed our cytopathology databases for an 11-year period to identify all fine-needle aspiration biopsy (FNAB) cases of palpable masses of the hand, wrist, ankle, or foot. Cases were included only if there was a subsequent tissue biopsy or a minimum 1-year clinical follow-up. Of 141 aspirates, 41, 23, 34, and 43 were from the hand, wrist, ankle, and foot, respectively. Specific benign or malignant diagnoses were achievable in 71.6% of cases, whereas the remaining cases were given a descriptive diagnosis (26.2%) or, infrequently, a "suspicious for" diagnosis (2.1%). Overall sensitivity and specificity for distinguishing a benign from malignant entity from all 4 sites were 100% and 96%, respectively, whereas positive and negative predictive... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202203 Mon, 25 Jan 2010 06:44:50 +0100 3202203 http://www.medworm.com/index.php?rid=3202202&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093232%26dopt%3DAbstract Authors: Syrjänen S, Naud P, Sarian L, Derchain S, Roteli-Martins C, Longatto-Filho A, Tatti S, Branca M, Erzen M, Hammes LS, Costa S, Syrjänen K To assess whether the potentially high-risk (HR) human papillomavirus (HPV)-related up-regulation of 14-3-3sigma (stratifin) has implications in the outcome of HPV infections or cervical intraepithelial neoplasia (CIN) lesions, cervical biopsy specimens from 225 women in the Latin American Screening Study were analyzed for 14-3-3sigma expression using immunohistochemical analysis. We assessed its associations with CIN grade and HR HPV at baseline and value in predicting outcomes of HR-HPV infections and the development of incident CIN 1+ and CIN 2+. Expression of 14-3-3sigma increased in parallel with the lesion grade. Up-regulation... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202202 Mon, 25 Jan 2010 06:44:47 +0100 3202202 http://www.medworm.com/index.php?rid=3202201&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093233%26dopt%3DAbstract Authors: Bechert CJ, Schnadig VJ, Payne DA, Dong J BK virus (BKV) is a nonenveloped, double-stranded DNA virus of the polyomavirus family that primarily affects immunocompromised people. BKV may cause nephropathy in renal transplant recipients receiving immunosuppressive therapy, resulting in renal dysfunction and, possibly, graft loss. Monitoring of BK viral load in urine and blood has been used as a surrogate marker of BKV nephropathy (BKVN). Although real-time polymerase chain reaction (PCR) is the method of choice, currently there is no US Food and Drug Administration-approved or standardized BK viral load assay. Different PCR assays vary significantly in sample types, DNA extraction method, PCR primers and probes, and reference materials used to generate a standard curve. These di... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202201 Mon, 25 Jan 2010 06:44:44 +0100 3202201 http://www.medworm.com/index.php?rid=3202200&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093234%26dopt%3DAbstract Authors: Vassalos CM, Spanakos G, Vassalou E, Papadopoulou C, Vakalis N Blastocystis is a polymorphic intestinal parasite that is common in humans. A total of 51 asymptomatic and symptomatic patients positive for Blastocystis only were included in the study. Symptoms were mainly nonspecific gastrointestinal symptoms. Blastocystis isolates were xenically cultured and subtyped. Blastocystis species subtype 3 was the predominant subtype. Intrasubtype differences (vacuolar/amoeboid presence) in subtype 3 morphotypes were observed in 32 asymptomatic and symptomatic subtype 3 cases and could possibly be related to Blastocystis pathogenic potential. Diverse morphologic features (vacuolar transiting to amoeboid), probably reflecting the progression from an asymptomatic to a symptomatic state, ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202200 Mon, 25 Jan 2010 06:44:42 +0100 3202200 http://www.medworm.com/index.php?rid=3202199&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093235%26dopt%3DAbstract Authors: Pitchford CW, Hettinga AC, Reichard KK Multiple studies, with differing results, have compared the added sensitivity of fluorescence in situ hybridization (FISH) with conventional cytogenetics (CC) to detect genetic abnormalities in myelodysplastic syndrome (MDS). We hypothesized that in the setting of an adequate CC study, FISH would correlate with microscopic genetic abnormalities involving chromosomes 5, 7, 8, and 20. We performed FISH for -5/5q, -7/7q, +8, and del(20q) on 102 MDS cases with normal CC (>/=20 consecutive metaphases) and on 35 MDS cases with abnormal CC. Of the 102 MDS cases with normal CC, only 1 was discrepant between FISH (showing +8) and CC (<1% of total cases). Of the 35 MDS cases with abnormal CC, 1 showed a minor discrepancy (-5 by CC vs del(5q) ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202199 Mon, 25 Jan 2010 06:44:39 +0100 3202199 http://www.medworm.com/index.php?rid=3202198&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093236%26dopt%3DAbstract Authors: Heerema-McKenney A, Waldron J, Hughes S, Zhan F, Sawyer J, Barlogie B, Shaughnessy JD We reviewed bone marrow studies from 351 multiple myeloma (MM) cases, selecting 12 cases (3.4%) with predominantly small lymphocyte-like morphologic features resembling B-cell lymphoma, and correlated their genetic and clinical features. All exhibited a diffuse interstitial pattern of marrow involvement. Small lymphocyte-like plasma cells were all CD45- with bright CD38 and CD138 expression and CD20 expression in 5 cases. No case had an increase in bone marrow B lymphocytes by flow cytometry. Of 12 cases, 9 were classified as the CD-2 molecular class by gene expression profiling (GEP). The 29 CD-2 class cases with (n = 9) and without (n = 20) small lymphocyte-like features could not be discer... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202198 Mon, 25 Jan 2010 06:44:36 +0100 3202198 http://www.medworm.com/index.php?rid=3202197&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093237%26dopt%3DAbstract Authors: Birgersdotter A, Baumforth KR, Wei W, Murray PG, Sjöberg J, Björkholm M, Porwit A, Ernberg I Connective tissue growth factor (CTGF) has a major role in development of fibrosis and in the wound-healing process. Microarray analysis of 44 classical Hodgkin lymphoma (cHL) samples showed higher CTGF messenger RNA expression in the nodular sclerosis (NS) than in the mixed cellularity (MC) subtype. When analyzed by immunohistochemical analysis, Hodgkin-Reed-Sternberg (H-RS) cells and macrophages in 23 cHLs and "popcorn" cells in 2 nodular lymphocyte predominant Hodgkin lymphomas showed expression of CTGF protein correlating with the extent of fibrosis. In NS, CTGF was also expressed in fibroblasts and occasional lymphocytes. Malignant cells in 32 samples of various non-Hodg... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202197 Mon, 25 Jan 2010 06:44:34 +0100 3202197 http://www.medworm.com/index.php?rid=3202196&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093238%26dopt%3DAbstract Authors: Matsumoto Y, Horiike S, Ohshiro M, Yamamoto M, Sasaki N, Tsutsumi Y, Kobayashi T, Shimizu D, Uchiyama H, Kuroda J, Nomura K, Shimazaki C, Taniwaki M The normal counterparts of peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) have not been accurately identified. We immunohistochemically analyzed 10 PTCL-NOS cases to examine the expression of the master regulators of T-cell differentiation and of surface antigens, including chemokine receptors. All cases were positive for the master regulator of helper T cells (Th-POK) and the marker of effector T cells (CD45RO). Three cases each were positive for T-Bet and GATA3, which are master regulators of helper T cells (T(h) ) type 1 (T(h)1) and 2 (T(h)2), respectively. Two cases were positive for the surface antigens of cen... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202196 Mon, 25 Jan 2010 06:44:31 +0100 3202196 http://www.medworm.com/index.php?rid=3202195&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093239%26dopt%3DAbstract Authors: De Smet D, Van Moer G, Martens GA, Nanos N, Smet L, Jochmans K, De Waele M The enumeration and identification of blood cells in body fluids offers important information for the diagnosis and treatment of various medical conditions. Manual microscopic methods (hemacytometer total cell count and cytocentrifuged differential count) have inherent analytic and economic disadvantages but are still considered the "gold standard" methods. We evaluated the analytic and clinical performance of the Cell-Dyn Sapphire hematology analyzer (Abbott Diagnostics Division, Santa Clara, CA) for automated blood cell counting and leukocyte differential counting in cerebrospinal fluid, serous fluid (peritoneal and pleural fluid), and continuous ambulatory peritoneal dialysis fluid, and we compared t... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202195 Mon, 25 Jan 2010 06:44:28 +0100 3202195 http://www.medworm.com/index.php?rid=3202194&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093240%26dopt%3DAbstract Authors: Chung HJ, Chi HS, Jang S, Park CJ We previously reported 24 cases of marrow fibrin-ring granuloma (FRG) encountered in 1 institution and concluded that, contrary to previous studies showing marrow FRG as a diagnostic marker for Q fever, Epstein-Barr virus (EBV) was the most common proven cause of such FRG. The present study characterized patients with EBV-associated marrow FRG. We retrospectively reviewed 17 cases of EBV-associated FRG (43% of 40 cases with marrow FRG) diagnosed by bone marrow biopsy. Patients with EBV-associated hemophagocytic lymphohistiocytosis (5/17 patients) and chronic active EBV infection (4/17) constituted 53% of patients with EBV-associated FRG. Three patients had lymphoma without marrow involvement. All patients except 1 presented first with fever; s... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202194 Mon, 25 Jan 2010 06:44:25 +0100 3202194 http://www.medworm.com/index.php?rid=3202193&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093241%26dopt%3DAbstract Authors: Chen Y, Han T, Iqbal J, Irons R, Chan WC, Zhu X, Fu K In diffuse large B-cell lymphoma (DLBCL), BCL2 expression usually correlates with the t(14;18) (q32;q21) in germinal center B-cell (GCB) subtype and with gain/amplification of chromosome 18q21 in the activated B cell-like subtype. Studies have suggested that the GCB subtype is less common in Chinese than in Western populations. We studied 124 Chinese DLBCL cases using immunohistochemical, conventional cytogenetics, and interphase fluorescence in situ hybridization analyses. A cohort of 114 well-characterized DLBCL cases from Western populations was also analyzed for comparison. Lower incidences of the GCB subtype (P = .0001) and the t(14;18) translocation (P = .0001) were present in Chinese cases. However, BCL2 overexpressi... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202193 Mon, 25 Jan 2010 06:44:22 +0100 3202193 http://www.medworm.com/index.php?rid=3202192&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093242%26dopt%3DAbstract Authors: Feng B, Verstovsek S, Jorgensen JL, Lin P Primary myelofibrosis (PMF) is a myeloproliferative neoplasm that may display a variable degree of cytopenia and dysplasia sometimes difficult to distinguish from myelodysplastic syndrome with myelofibrosis (MDS-MF). We reviewed flow cytometric features of bone marrow from 70 cases of PMF and compared them with those from 17 cases of MDS-MF and 20 nonneoplastic control cases. The results were correlated with JAK2(V617F) and cytogenetic findings. Granulocytes and monocytes from PMF cases exhibited multiple dysplastic features overlapping with those of MDS-MF at a comparable or higher frequency: low side scattering, aberrant CD56 expression in granulocytes and monocytes, and an abnormal CD13/CD16 maturation pattern. Unique to PMF was the... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202192 Mon, 25 Jan 2010 06:44:20 +0100 3202192 http://www.medworm.com/index.php?rid=3202191&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093243%26dopt%3DAbstract Authors: Ott HW, Griesmacher A, Schnapka-Koepf M, Golderer G, Sieberer A, Spannagl M, Scheibe B, Perkhofer S, Will K, Budde U Analysis of von Willebrand factor (vWF) multimers allows classification of the subtypes of von Willebrand disease (vWD) in human serum and platelet lysates. A novel method for multimer analysis of vWF by 2-chamber, vertical (sodium dodecyl sulfate), agarose gel electrophoresis, designed for comparing discontinuous high- and low-resolving gels for plasma and platelets, followed by Western blotting and high-sensitivity fluorescence detection (HSFD) of cyanine (Cy)5-labeled vWF multimers is presented. HSFD shows that this method has high discriminatory power for visualization and densitometric analysis of platelets and plasma vWF multimers in various types of vWD a... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202191 Mon, 25 Jan 2010 06:44:17 +0100 3202191 http://www.medworm.com/index.php?rid=3202190&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093244%26dopt%3DAbstract Authors: Raijmakers MT, Menting CH, Vader HL, van der Graaf F The Clinical and Laboratory Standards Institute (CLSI) recently abandoned its recommendation for drawing a discard tube when performing a prothrombin time (PT)/international normalized ratio (INR) or an activated partial thromboplastin time (APTT). Because there is currently no evidence that a discard tube is necessary for more specialized coagulation assays, we studied the need for a discard tube for some of these tests. Blood was obtained from 88 subjects in 2 subsequent citrate tubes. Platelet-free plasma was tested for PT, APTT, antithrombin, protein C, and factors II, V, VIII, IX, and X. Difference and bias between tubes were tested using the Wilcoxon signed rank test and Bland-Altman plots. For only APTT, antithrombin,... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202190 Mon, 25 Jan 2010 06:44:14 +0100 3202190 http://www.medworm.com/index.php?rid=3202189&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093245%26dopt%3DAbstract Authors: Lee SH, Moriarty AT, Cox JT, Castle PE PMID: 20093245 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202189 Mon, 25 Jan 2010 06:44:11 +0100 3202189 http://www.medworm.com/index.php?rid=3202188&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093246%26dopt%3DAbstract Authors: Garfield D PMID: 20093246 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202188 Mon, 25 Jan 2010 06:44:09 +0100 3202188 http://www.medworm.com/index.php?rid=3202187&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20093247%26dopt%3DAbstract Authors: Slater L PMID: 20093247 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3202187 Mon, 25 Jan 2010 06:44:06 +0100 3202187 http://www.medworm.com/index.php?rid=3111913&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023253%26dopt%3DAbstract Authors: Hernandez JS, Dale JC, Bennet KE, Varkey P Because the practice of pathology and laboratory medicine evolves rapidly, laboratory medical directors must constantly introduce new tests and services and continue to provide consistent, reliable results for existing tests. Innovations in laboratory medicine are frequently introduced, and the number of commercial vendors of test kits and reagents increases yearly. These innovations, however, may pose barriers to standardization and integration of laboratories and to interpretation of results generated by different laboratories. We propose a practical framework for medical directors to address the seemingly contradictory challenges of standardizing and integrating while simultaneously providing the flexibility to introduce innovation... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111913 Tue, 22 Dec 2009 20:08:57 +0100 3111913 http://www.medworm.com/index.php?rid=3111912&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023254%26dopt%3DAbstract We examined the effect of renal dysfunction on B-natriuretic peptide (BNP), N-terminal (NT)-proBNP, and their molar ratio at varying severities of cardiac function in 94 Thai patients with chest pain (52 men; 32 women), also measuring creatinine and left ventricular ejection fraction (LVEF). Renal function was classified into 5 stages by estimated glomerular filtration rate. The molar NT-proBNP/BNP ratio was calculated. Cardiac status was classified by LVEF (normal, >50%; moderate, 35%-50%; severe, <35%). BNP, NT-proBNP, and their ratio corresponded to renal disease stage exponential (0.51, 1.05, and 0.54, respectively; correlation coefficients, >/=0.95). BNP and the ratio are affected less than NT-proBNP by renal dysfunction, starting in stage III; NT-proBNP expresses effects sta... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111912 Tue, 22 Dec 2009 20:08:54 +0100 3111912 http://www.medworm.com/index.php?rid=3111911&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023255%26dopt%3DAbstract Authors: Rutledge J, Xu M, Simpson J To meet the increased clinical demands of our hospital expansion, improve quality, and reduce costs, our tertiary care, pediatric core laboratory used the Toyota Production System lean processing to reorganize our 24-hour, 7 d/wk core laboratory. A 4-month, consultant-driven process removed waste, led to a physical reset of the space to match the work flow, and developed a work cell for our random access analyzers. In addition, visual controls, single piece flow, standard work, and "5S" were instituted. The new design met our goals as reflected by achieving and maintaining improved turnaround time (TAT; mean for creatinine reduced from 54 to 23 minutes) with increased testing volume (20%), monetary savings (4 full-time equivalents), decreased variab... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111911 Tue, 22 Dec 2009 20:08:52 +0100 3111911 http://www.medworm.com/index.php?rid=3111910&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023256%26dopt%3DAbstract Authors: Luo J, Qi C, Xu W, Kamel-Reid S, Brandwein J, Chang H Mutations in the nucleophosmin (NPM1) exon 12 resulting in delocalization of NPM1 into the cytoplasm occur in 50% to 60% of acute myeloid leukemia cases with a normal karyotype (AML-NK). As recent studies suggest such patients have a favorable prognosis and there are discordant reports of the immunohistochemical detection of cytoplasmic NPM1 (NPMc+) for predicting NPM1 gene mutations, we correlated the immunohistochemical detection of NPMc+, NPM1 gene mutations, and prognosis in 57 cases of AML-NK. All 31 NPMc+ cases (54% of total) had NPM1 mutations, but none of the 26 nucleus-restricted (NPMc-) cases (46% of total) had NPM1 mutations (P < .0001). NPM1 mutations were correlated with FLT3-internal tandem duplication (ITD... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111910 Tue, 22 Dec 2009 20:08:49 +0100 3111910 http://www.medworm.com/index.php?rid=3111909&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023257%26dopt%3DAbstract Authors: Nasr MR, Rosenthal N, Syrbu S The optimal use of transcription factors to determine B-lineage specificity in B-acute lymphoblastic leukemia/lymphoma (B-ALL) has not been fully investigated. We undertook an extensive immunohistochemical study of a panel of B-cell transcription factors in B- and T-ALL and Burkitt lymphoma to evaluate those with the best specificity and sensitivity. Tissue microarrays were constructed from 34 B-ALL, 19 T-ALL, and 30 Burkitt lymphoma samples. All 34 (100%) cases of B-ALL expressed PAX5; 32 (94%), BOB.1; 33 (97%), PU.1; 29 (85%), CD79a; 27 (79%), CD22; 2 (6%), CD20; 9 (26%), OCT-2; and 3 (9%), MUM1. Burkitt lymphoma cases were positive for PAX5 (30/30 [100%]), BOB.1 (27/30 [90%]), PU.1 (23/30 [77%]), CD79a (29/30 [97%]), CD22 (14/30 [47%]), CD20 (3... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111909 Tue, 22 Dec 2009 20:08:46 +0100 3111909 http://www.medworm.com/index.php?rid=3111908&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023258%26dopt%3DAbstract We report 4 cases of extranodal FDCS, 2 cases in the nasopharynx that were diagnosed as undifferentiated carcinomas because they were characterized by syncytial epithelial cells with sheet or nest-like distribution and 2 cases in the tonsil and soft palate that were characterized by vaguely concentric whorls consisting of spindle to ovoid cells. The latter case was diagnosed as ectopic meningioma. The analysis of all cases from the literature and ours shows that 58% (21/36) of the cases are misdiagnosed initially, often as undifferentiated carcinoma or meningioma, which the differential diagnoses should be mostly focused on. With a median follow-up of 27 months, the recurrence, metastasis, and mortality rates are 23%, 21%, and 3%, respectively, suggesting that extranodal FDCS of the pharyn... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111908 Tue, 22 Dec 2009 20:08:44 +0100 3111908 http://www.medworm.com/index.php?rid=3111907&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023259%26dopt%3DAbstract Authors: Geyer JT, Ferry JA, Longtine JA, Flotte TJ, Harris NL, Zukerberg LR Primary cutaneous marginal zone lymphoma (MZL) is a common B-cell lymphoma of skin and is characterized by an infiltrate of neoplastic marginal zone B cells typically within the marginal zones of reactive lymphoid follicles and the interfollicular region. However, in our experience, many cases have underemphasized features such as marked plasmacytic differentiation and/or a prominent T-cell component, which may obscure the neoplastic B cells and lead to misdiagnosis. We wanted to draw attention to these features and have studied 15 cases of MZL with marked plasmacytic differentiation, 10 of which had numerous T cells, some with cytologic atypia, and few B cells in the interfollicular region. Plasma cells were ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111907 Tue, 22 Dec 2009 20:08:41 +0100 3111907 http://www.medworm.com/index.php?rid=3111906&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023260%26dopt%3DAbstract Authors: Chang H, Jiang AM, Qi CX Hemizygous TP53 gene deletion is the most important adverse risk factor in chronic lymphocytic leukemia (CLL), but its relationship with p53 protein expression is unclear. We investigated 110 CLL cases and correlated nuclear p53 protein immunoreactivity with TP53 gene deletion status and other CLL-associated genetic risk factors. Fluorescence in situ hybridization detected hemizygous TP53 deletions in 15 cases (13.6%), whereas immunohistochemical analysis detected nuclear p53 protein expression in 14 (12.7%). All cases expressing nuclear p53 protein had hemizygous TP53 deletions. Hemizygous TP53 gene deletion and p53 protein expression were strongly correlated (P < .001). There was no association between p53 expression and del(13q), del(11q) or tris... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111906 Tue, 22 Dec 2009 20:08:38 +0100 3111906 http://www.medworm.com/index.php?rid=3111905&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023261%26dopt%3DAbstract We describe 6 adults with secondary t-ALL without 11q23 abnormalities following various treatment regimens for primary malignancies. We also reviewed 48 t-ALL cases, with complete chromosomal karyotyping, reported in the literature from 1992 to 2007. In the 48 cases, an 11q23 abnormality involving the MLL gene locus was the predominant chromosomal aberration (32 [67%]), followed by t(9;22) (6 [13%]) and a normal karyotype (4 [8%]). Compared with t-ALL cases with an 11q23 abnormality, cases without an 11q23 abnormality had a relatively longer latency period (median, 36 vs 19 months) and a different primary malignancy spectrum. No major difference was observed between groups in regard to age, sex, or receipt of a topoisomerase II inhibitor. The t(8;14)(q11.2;q32), a rare, nonrandom, balanced... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111905 Tue, 22 Dec 2009 20:08:36 +0100 3111905 http://www.medworm.com/index.php?rid=3111904&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023262%26dopt%3DAbstract Authors: Colorado M, Cuadrado MA, Insunza A, Mazorra F, Acinas O, Iriondo A We evaluated the validity and accuracy of cytomorphology and multiparametric flow cytometry (C-FCM) in diagnosing oncohematologic disease in 223 consecutive lymph node biopsy specimens from patients with lymphadenopathy, from 2004 to 2007. C-FCM and histopathologic studies were interpreted independently by hematologists and pathologists, respectively. C-FCM detected neoplastic disorders in 133 samples (59.6%): 92 non-Hodgkin lymphomas (NHLs; 41.3%), 21 Hodgkin lymphomas (HLs; 9.4%), 19 malignant nonhematologic neoplasms (8.5%), and 1 multiple myeloma (0.4%). Sensitivity and specificity were 87.25% and 95.95%, respectively. Positive predictive value and negative predictive value (NPV) were 97.74% and 78.89%, res... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111904 Tue, 22 Dec 2009 20:08:33 +0100 3111904 http://www.medworm.com/index.php?rid=3111903&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023263%26dopt%3DAbstract Authors: Cioc AM, Wagner JE, Macmillan ML, Defor T, Hirsch B Predisposition to myelodysplastic syndrome (MDS) and acute leukemia is a hallmark of Fanconi anemia (FA). Morphologic criteria for MDS in FA are not well established, nor is the significance of clonal chromosomal abnormalities. We reviewed bone marrow samples of 119 FA patients: 23 had MDS, with the most common subtype refractory cytopenia with multilineage dysplasia. The presence of MDS was highly correlated with the presence of clonal abnormalities. Neutrophil dysplasia and increased blasts were always associated with the presence of a clone, in contrast with dyserythropoiesis. The most frequent clones had gains of 1q and 3q and/or loss of 7. Karyotype complexity also correlated with MDS. One third of patients with 3q as a ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111903 Tue, 22 Dec 2009 20:08:31 +0100 3111903 http://www.medworm.com/index.php?rid=3111902&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023264%26dopt%3DAbstract Authors: Monajemzadeh M, Shahsiah R, Zarei A, Alamooti AA, Mahjoub F, Mamishi S, Khotai G, Pazira R, Eram N Vaccination of all newborns with bacille Calmette-Guérin (BCG) vaccine is a standard practice in developing countries. Disseminated mycobacterial infection in an immunocompromised child can be caused by BCG and other mycobacteria. A total of 21 patients with a histopathologic diagnosis of mycobacterial infection were studied in a period of 4 years. DNA was extracted from formalin-fixed, paraffin-embedded tissues. Real-time polymerase chain reaction was performed to determine the mycobacterial species. The overall sensitivity of the assay was 71.5%. The prevalence rates of BCG, Mycobacterium tuberculosis, and other mycobacteria in the positive results were 80% (12/15), 13% (2... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111902 Tue, 22 Dec 2009 20:08:28 +0100 3111902 http://www.medworm.com/index.php?rid=3111901&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023265%26dopt%3DAbstract Authors: Reller ME, Alcabasa RC, Lema CA, Carroll KC We compared 3 rapid assays for Clostridium difficile with a cell culture cytotoxicity neutralization assay (CCNA). Of 600 stool samples, 46 were positive for toxigenic C difficile. Both rapid common antigen assays were highly sensitive (91.3%-100%) and, therefore, were appropriate screening tests. The rapid toxin assay had poor sensitivity (61%) but excellent specificity (99.3%). Testing stools for glutamate dehydrogenase (step 1) and those positive with a rapid toxin assay (step 2) would correctly classify 81% of submitted specimens within 2 hours, including during periods of limited staffing (evenings, nights, and weekends). CCNA could then be used as a third step to test rapid toxin-negative samples, thereby providing a final resu... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111901 Tue, 22 Dec 2009 20:08:26 +0100 3111901 http://www.medworm.com/index.php?rid=3111900&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023266%26dopt%3DAbstract Authors: Cavalieri SJ, Hrabovsky S, Jorgensen T The new MONOLISA Bio-Rad kits were compared to DiaSorin kits for the detection of hepatitis B virus (HBV) total core (HBcTAb) and surface (HBsAb) antibodies on the Bio-Rad Evolis immunoanalyzer. The resolved sensitivities, specificities, positive and negative predictive values, and overall accuracy were 91%, 100%, 100%, 96%, and 97%, respectively, for HBcTAb and 99%, 95%, 96%, 99%, and 97%, respectively, for HBsAb. Whereas accuracy and reagent cost were comparable between the kits, Bio-Rad kits required less specimen volume and less instrument processing time to results than the DiaSorin kits. PMID: 20023266 [PubMed - as supplied by publisher] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111900 Tue, 22 Dec 2009 20:08:23 +0100 3111900 http://www.medworm.com/index.php?rid=3111899&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023267%26dopt%3DAbstract Authors: Brunelli M, Delahunt B, Gobbo S, Tardanico R, Eccher A, Bersani S, Cossu-Rocca P, Parolini C, Balzarini P, Menestrina F, Cheng L, Eble JN, Martignoni G We investigated the usefulness of interphase fluorescence in situ hybridization (FISH) analysis to differentiate between 11 chromophobe renal carcinomas and 12 renal oncocytomas, showing different clinical outcomes, when compared with conventional metaphase cytogenetics by karyotyping. Karyotypically, 3 chromophobe renal cell carcinomas showed losses of chromosomes, 3 were polyploid, 1 was normal, and 4 failed to grow. Of 12 oncocytomas, 5 showed a normal numeric karyotype and 6 additional structural rearrangements. FISH on chromophobe renal cell carcinomas showed a high percentage of cases (10/11 [91%]) with multiple numeric l... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111899 Tue, 22 Dec 2009 20:08:21 +0100 3111899 http://www.medworm.com/index.php?rid=3111898&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023268%26dopt%3DAbstract We describe our experience in a 6-year period and discuss the pathologist's role. Liver biopsies of 1,842 patients with hepatitis B or C, for the 2001-2007 period at the University Health Network, Toronto, Canada, were reviewed; 410 other diagnoses were documented in 377 patients (20.5%; mean age, 25.4 years; range, 15-80 years). These diagnoses included 58 hepatocellular carcinomas and 16 dysplastic nodules, which are recognized complications of chronic viral hepatitis. The remaining findings included the following: steatosis/steatohepatitis, 251; hemosiderosis, 62; granulomatous disease, 7; drug-induced hepatitis, 4; primary biliary cirrhosis, 3; Wilson disease, 2; metastases, 2; and cholangiocarcinoma, atypical lymphoid proliferation, alpha(1)-antitrypsin deficiency, cystic fibrosis, an... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111898 Tue, 22 Dec 2009 20:08:18 +0100 3111898 http://www.medworm.com/index.php?rid=3111897&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023269%26dopt%3DAbstract Authors: Solis LM, Raso MG, Kalhor N, Behrens C, Wistuba II, Moran CA Sixteen cases of primary oncocytic adenocarcinomas of the lung are reported. The patients were 11 women and 5 men between the ages of 47 and 81 years (median, 75 years) with symptoms of cough, chest pain, and shortness of breath. Surgical staging disclosed 14 patients (88%) with stage I disease, 1 (6%) with stage II, and 1 (6%) with stage III. Histologically, all the cases displayed prominent oncocytic features with conventional growth patterns, including acinar, papillary, and bronchioloalveolar. Immunohistochemically, the tumors displayed positive staining for keratin 7, thyroid transcription factor-1, and mitochondrial antibody. Molecular studies showed 3 (20%) of 15 tumors with EGFR mutations and 3 additional cas... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111897 Tue, 22 Dec 2009 20:08:16 +0100 3111897 http://www.medworm.com/index.php?rid=3111896&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023270%26dopt%3DAbstract The objectives of this study were to evaluate BRAF mutations in GISTs and then to correlate BRAF mutational status in the tumor with clinical parameters, with B-raf expression, and with activation of some cellular pathways. BRAF mutation was screened in 321 GISTs with 70 wild-type GISTs. BRAF V600E was detected in 9 (13%) of 70 wild-type GISTs. No mutations were detected in GISTs bearing KIT or PDGFRA mutations. BRAF V600E detection in the tumor does not induce a higher expression of the B-raf protein or the preferential activation of the p42/44 mitogen-activated protein kinase (MAPK) signaling pathway compared with GISTs without the BRAF mutation. In comparison with the GIST group with KIT or PDGFRA mutation or the wild-type GIST group without BRAF mutation, the wild-type GIST group with ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111896 Tue, 22 Dec 2009 20:08:13 +0100 3111896 http://www.medworm.com/index.php?rid=3111895&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023271%26dopt%3DAbstract Authors: Battochio A, Mohammed S, Winthrop D, Lefresne S, Mulder K, Chu Q, O'Hara C, Lai R Mutational analysis of c-KIT or PDGFRA has become an important laboratory assay for patients with gastrointestinal stromal tumors (GISTs) because the results are useful in predicting the responsiveness to imatinib. To assess the diagnostic usefulness of denaturing high-pressure liquid chromatography (DHPLC) in this setting, we performed DHPLC and DNA sequencing to study exons 9, 11, 13, and 17 of c-KIT and exons 12 and 18 of PDGFRA in 54 consecutive cases of GIST collected from a single population. Most (40/54 [74%]) carried c-KIT mutations, and 7 (13%) carried PDGFRA mutations. These results were similar to those described in the literature. It is important to note that DHPLC was found to be hig... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111895 Tue, 22 Dec 2009 20:08:10 +0100 3111895 http://www.medworm.com/index.php?rid=3111894&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023272%26dopt%3DAbstract We describe our experience with voice recognition-integrated synoptic-like dictation, associating templates with key spoken phrases, that we have used in gross examination of common specimens and as a major component of our workflow since 2001. The primary application is VoiceOver Enterprise (Voicebrook, Lake Success, NY), which uses Dragon NaturallySpeaking Medical Edition (Nuance Communications, Burlington, MA) as its speech engine. This integrates with the anatomic pathology laboratory information system (APLIS) and other applications, such as Microsoft Office (Microsoft, Redmond, WA). The largest user group, pathology assistants, mainly dictates biopsy reports, numbering approximately 210,000 specimens since 2001. The technology has been useful in our anatomic pathology workflow and pr... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111894 Tue, 22 Dec 2009 20:08:08 +0100 3111894 http://www.medworm.com/index.php?rid=3111893&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20023273%26dopt%3DAbstract Authors: Hemker HC, De Smedt E, Chandler WL, Roshal M PMID: 20023273 [PubMed - as supplied by publisher] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3111893 Tue, 22 Dec 2009 20:08:05 +0100 3111893 http://www.medworm.com/index.php?rid=3017438&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926570%26dopt%3DAbstract Authors: Wick MR PMID: 19926570 [PubMed - as supplied by publisher] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017438 Mon, 23 Nov 2009 04:51:04 +0100 3017438 http://www.medworm.com/index.php?rid=3017437&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926571%26dopt%3DAbstract Authors: Shirts BH, Gundlapalli AV, Jackson B Electronic medical records have the ability to link to reference material, providing clinicians with immediate access to information relevant to patient care. Adding relevant links to laboratory test results could add value while minimizing the volume of ancillary text presented. We provided Web-based universal resource locator (URL) links with all results of 7 laboratory tests ordered at ARUP Laboratories (Salt Lake City, UT). URL links provided were modified 7 months later, and use between initial and subsequent URLs was tracked to establish frequency and duration of access to supplemental Web information. Monthly Web-site hit rates for individual tests varied from 0.00% to 3.00% (median, 0.12%). Rare and specialty tests averaged higher h... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017437 Mon, 23 Nov 2009 04:51:01 +0100 3017437 http://www.medworm.com/index.php?rid=3017436&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926572%26dopt%3DAbstract Authors: Schreiber WE, Giustini DM In the past few years, the term Web 2.0 has become a descriptor for the increased functionality of Web sites, including those with medical content. Most physicians do not know what Web 2.0 means or how it can impact their work lives. This review provides some background on the evolution of Web 2.0 and describes how its features are being incorporated into medical Web sites. Some potential applications of Web 2.0 in pathology and laboratory medicine are discussed, as are the issues that must be considered when adopting this new technology. PMID: 19926572 [PubMed - as supplied by publisher] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017436 Mon, 23 Nov 2009 04:50:58 +0100 3017436 http://www.medworm.com/index.php?rid=3017435&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926573%26dopt%3DAbstract Authors: Burford H, Baloch Z, Liu X, Jhala D, Siegal GP, Jhala N Pancreatic endocrine neoplasm (PEN) and solid pseudopapillary neoplasm of the pancreas (SPN) frequently pose diagnostic challenges. We sought to determine which markers could provide the best immunophenotypic characterization of PEN and SPN, allowing separation on limited cytology samples. We retrieved 22 resected PEN (n = 12) and SPN (n = 10) tumors to serve as a training set for the performance of extensive immunohistochemical staining. Based on these results, we selected a subset of antibodies for application to 25 fine-needle aspiration (FNA) samples from PEN (n = 16) and SPN (n = 9). Chromogranin A, synaptophysin, CD56, and progesterone receptor (PR) highlighted PEN cases in the training set; E-cadherin was noted in ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017435 Mon, 23 Nov 2009 04:50:55 +0100 3017435 http://www.medworm.com/index.php?rid=3017434&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926574%26dopt%3DAbstract We examined the influence of human papillomavirus (HPV) L1 capsid protein detection in a randomized, prospective study of 187 high-risk HPV+ early dysplastic lesions during 36 to 46 months. The difference in the clinical outcome of the HPV L1- cases and the HPV L1+ cases was highly statistically significant (P < .0001) and independent of the classification of low-grade squamous intraepithelial lesion (mild dysplasia) and high-grade squamous intraepithelial lesion of the moderate dysplastic type. L1+ mild and moderate dysplasias, reflecting productive HPV infection, showed low malignant potential, justifying a wait-and-watch strategy to prevent overtreatment, especially in young women. L1- early dysplastic lesions, as nonproductive infections or precancerous lesions, have a high malignan... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017434 Mon, 23 Nov 2009 04:50:52 +0100 3017434 http://www.medworm.com/index.php?rid=3017433&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926575%26dopt%3DAbstract Authors: Derycke MS, Andersen JD, Harrington KM, Pambuccian SE, Kalloger SE, Boylan KL, Argenta PA, Skubitz AP We sought to investigate the expression levels of S100A1 in ovarian cancer cell lines and tissues to correlate S100A1 with subtype, stage, grade, and relapse-free survival. S100A1 messenger RNA and protein were up-regulated in ovarian cancer cell lines and tumors compared with normal ovarian cell lines and tissues by gene microarray analysis, reverse transcriptase-polymerase chain reaction, quantitative reverse transcriptase-polymerase chain reaction, and Western immunoblotting. In the study, 63.7% of serous, 21.2% of clear cell, 11.2% of endometrioid, and 3% of mucinous ovarian (1/31) cancers were S100A1+ by immunohistochemical staining of tissue microarrays (n = 500). S100A1... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017433 Mon, 23 Nov 2009 04:50:50 +0100 3017433 http://www.medworm.com/index.php?rid=3017432&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926576%26dopt%3DAbstract Authors: Wong NL, Di F Nodular fasciitis (NF), proliferative fasciitis (PF), and proliferative myositis (PM) are pseudosarcomatous lesions that typically resolve spontaneously. We previously reported the feasibility of diagnosing this family of lesions by fine-needle aspiration cytology (FNAC) based on 17 cases. The present study included 52 new cases (NF, 46; PF, 3; PM, 3) diagnosed by FNAC at Kiang Wu Hospital, Macau, 2001 to 2007, to validate the diagnostic features. All lesions appeared as recent-onset, small, superficially located, rapidly growing nodules. In 88% of cases (46/52: NF, 41; PF, 2; PM, 3), spontaneous resolution occurred in 1 to 16 weeks (median, 2 weeks) after FNAC diagnosis. FNAC smears were characterized by a hypercellular and polymorphic pattern of lesional cells ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017432 Mon, 23 Nov 2009 04:50:47 +0100 3017432 http://www.medworm.com/index.php?rid=3017431&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926577%26dopt%3DAbstract Authors: Montone KT Fusarium and Aspergillus are 2 genera of fungal pathogens that can result in devastating disease particularly in immunosuppressed hosts. In tissue sections, these organisms can be extremely difficult to distinguish from one another. To differentiate between these 2 pathogens, a rapid (<3 hours) ribosomal RNA (rRNA) in situ hybridization (ISH) protocol using dual fluorogenic-labeled oligonucleotide probes composed of a mixture of DNA and locked nucleic acids (LNAs) was developed. This assay was able to differentiate between Aspergillus and Fusarium in formalin-fixed, paraffin-embedded tissue sections. ISH targeting rRNA can be used to identify the species of fungal pathogens in surgical pathology material and may be useful when pathogens are histologically observe... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017431 Mon, 23 Nov 2009 04:50:44 +0100 3017431 http://www.medworm.com/index.php?rid=3017430&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926578%26dopt%3DAbstract In this study, we analyzed loss of heterozygosity (LOH) in 10 cases of coexistent ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS), and invasive carcinoma. DNA from the separate components of each lesion was subjected to LOH analysis using 13 markers on 7 chromosomes. In 7 cases, the DCIS and LCIS shared loss of a common allele, suggesting a clonal relationship. The invasive component shared loss of the same allele in 5 tumors. This finding indicates that coexistent lobular and ductal carcinomas exhibit shared genetic abnormalities, contradicting the conventional concept that these lesions represent separate, exclusive pathways of breast neoplasia. Instead, these traditionally segregated classes of breast cancer may, in fact, share common precursor lesions. PMID: 19926... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017430 Mon, 23 Nov 2009 04:50:41 +0100 3017430 http://www.medworm.com/index.php?rid=3017429&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926579%26dopt%3DAbstract Authors: Cardona DM, Zhang X, Liu C Carbamoyl phosphate synthetase I (CPS1), normally found in hepatocytes and small-intestine (SI) enterocytes, is the antigen of Hep Par 1 antibody. Expression of CPS1 in invasive SI adenocarcinoma seems to be lost. We retrospectively collected 36 total specimens, which included 31 SI adenomas and 21 adenocarcinomas. We used 34 cases of duodenitis as a control group. Immunohistochemical and Western blot analyses were performed to determine CPS1 expression. The normal SI mucosa, all 34 cases of duodenitis, and all 29 adenomas with low-grade dysplasia demonstrated diffuse Hep Par 1 expression. Of the 21 invasive adenocarcinomas, 15 lost antigen expression (71%). These data are statistically significant (P < .05). Western blot analysis confirmed the im... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017429 Mon, 23 Nov 2009 04:50:38 +0100 3017429 http://www.medworm.com/index.php?rid=3017428&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926580%26dopt%3DAbstract Authors: Syrjänen S, Naud P, Sarian L, Derchain S, Roteli-Martins C, Longatto-Filho A, Tatti S, Branca M, Erzen M, Hammes LS, Costa S, Syrjänen K Protease inhibitor serpin-B2 (plasminogen activator inhibitor-2 [PAI-2]) protects pRb from degradation in human papillomavirus (HPV)-18+ HeLa cells. Our objective was to assess whether the pRb-mediated HPV-suppressive effect of PAI-2 in cancer cell lines has implications in the outcome of HPV infections. Cervical biopsy specimens from 225 women were analyzed for PAI-2 expression to assess its value as a predictor of cervical intraepithelial neoplasia (CIN) grade, high-risk (HR) HPV at baseline, outcomes of HR-HPV infections, and the development of incident CIN. PAI-2 expression increased in parallel with lesion grade. Nuclear PAI-2 ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017428 Mon, 23 Nov 2009 04:50:35 +0100 3017428 http://www.medworm.com/index.php?rid=3017427&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926581%26dopt%3DAbstract Authors: Sasaki K, Bastacky SI, Zynger DL, Parwani AV CD10 has recently been described as a marker that can distinguish wolffian duct derivatives from müllerian remnants but has yet to be tested in vasectomy specimens. We sought to determine if CD10 and pankeratin could corroborate the presence of vas deferens (VD). For the study, 103 consecutive vasectomy specimens were immunohistochemically analyzed for CD10, pankeratin, and CD31 expression in luminal and basal layer cells. In all cases with optimal epithelial histologic features (92/103), CD10 demonstrated intense apical membranous expression in all VD and weak basal cytoplasmic staining in about 98% of cases. Pankeratin demonstrated cytoplasmic and membranous expression in apical and basal layers in 99% of VD. In cases with su... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017427 Mon, 23 Nov 2009 04:50:32 +0100 3017427 http://www.medworm.com/index.php?rid=3017426&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926582%26dopt%3DAbstract This study evaluated TMA assessment of breast biomarkers using immunohistochemical analysis in a clinical histopathology laboratory. Performance parameters, interobserver variability, and concordance between TMA and whole section results were assessed. The arrays had few lost or noninformative cores. A loss of stain intensity occurred in the arrays compared with the whole sections with some but not all antibodies, highlighting the need to validate the staining protocol for each antibody used on TMA sections. With recommended guidelines for specimen selection and reporting, TMA was found to be an economical replacement for whole section analysis for breast biomarkers. PMID: 19926582 [PubMed - as supplied by publisher] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017426 Mon, 23 Nov 2009 04:50:29 +0100 3017426 http://www.medworm.com/index.php?rid=3017425&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926583%26dopt%3DAbstract Authors: Nasr MR, Mukhopadhyay S, Zhang S, Katzenstein AL An association between Hashimoto thyroiditis and papillary thyroid carcinoma has been postulated for decades. We undertook this study to identify potential precursors of papillary thyroid carcinoma in Hashimoto thyroiditis using a combination of morphologic, immunohistochemical, and molecular techniques. For the study, samples from 59 cases of Hashimoto thyroiditis were stained with antibodies to HBME1 and cytokeratin (CK)19. Tiny HBME1+ and CK19+ atypical cell clusters were identified and analyzed for the BRAF mutation by the colorimetric Mutector assay and allele-specific polymerase chain reaction. HBME1+ and CK19+ atypical cell clusters were identified in 12 (20%) of 59 cases. The minute size (<1 mm) of the clusters and th... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017425 Mon, 23 Nov 2009 04:50:26 +0100 3017425 http://www.medworm.com/index.php?rid=3017424&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926584%26dopt%3DAbstract Authors: Melanson SE, Goonan EM, Lobo MM, Baum JM, Paredes JD, Santos KS, Gustafson ML, Tanasijevic MJ Our goals were to improve the overall patient experience and optimize the blood collection process in outpatient phlebotomy using Lean principles. Elimination of non-value-added steps and modifications to operational processes resulted in increased capacity to handle workload during peak times without adding staff. The result was a reduction of average patient wait time from 21 to 5 minutes, with the goal of drawing blood samples within 10 minutes of arrival at the phlebotomy station met for 90% of patients. In addition, patient satisfaction increased noticeably as assessed by a 5-question survey. The results have been sustained for 10 months with staff continuing to make process impr... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017424 Mon, 23 Nov 2009 04:50:23 +0100 3017424 http://www.medworm.com/index.php?rid=3017423&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926585%26dopt%3DAbstract Authors: Korpi-Steiner NL, Williamson EE, Karon BS We compared the clinical concordance of estimated glomerular filtration rate (eGFR) based on 3 whole blood creatinine assays with the eGFR calculated from a reference plasma creatinine assay. Whole blood creatinine on the Radiometer ABL800 FLEX (Radiometer A/S, Bronshoj, Denmark) demonstrated the best correlation and concordance to plasma creatinine/eGFR compared with the i-STAT (i-STAT, East Windsor, NJ) and StatSensor (Nova Biomedical, Waltham, MA). The i-STAT had better sensitivity (compared with Radiometer) but poorer specificity for prediction of plasma eGFR less than 60 mL/min/1.73 m(2). The StatSensor demonstrated lower concordance of whole blood to plasma eGFR but offered a slope and an intercept offset feature that partially c... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017423 Mon, 23 Nov 2009 04:50:20 +0100 3017423 http://www.medworm.com/index.php?rid=3017422&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926586%26dopt%3DAbstract We present a detailed case analysis of a CLL/SLL with metachronous IDCS and demonstrate that these 2 neoplasms are clonally related. The IDCS and CLL cells had trisomy 12 and identical monoclonal immunoglobulin heavy chain gene rearrangements. Analysis of transcription factors with a role in myeloid differentiation demonstrated PU.1 up-regulation and C/EBPalpha down-regulation in IDCS compared with CLL. High-density array comparative genomic hybridization also identified gains in part of chromosome 16q in IDCS. Our study demonstrates for the first time clonal transformation of CLL/SLL into IDCS. This phenomenon may be triggered by alterations in lineage-determining transcription programs, which result in transdifferentiation, coupled with additional oncogenic stimuli caused by chromosomal ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017422 Mon, 23 Nov 2009 04:50:17 +0100 3017422 http://www.medworm.com/index.php?rid=3017421&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926587%26dopt%3DAbstract Authors: Seegmiller AC, Kroft SH, Karandikar NJ, McKenna RW Morphologic distinction of leukemic lymphoblasts in B acute lymphoblastic leukemia (B-ALL) from their nonneoplastic counterparts in bone marrow (hematogones) can be difficult. Thus, the presence of aberrant antigen expression detectable by flow cytometry may be critical for diagnosis of B-ALL and detection of minimal residual disease. The current study examined the immunophenotype of B-lineage leukemic lymphoblasts in 200 consecutive, unique, pretreatment patient specimens. We found that all cases of B-ALL exhibited multiple immunophenotypic aberrancies by which they can be distinguished from hematogones. The most frequent aberrancies were uniform or a spectrum of expression of terminal deoxynucleotidyl transferase and CD34, u... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017421 Mon, 23 Nov 2009 04:50:14 +0100 3017421 http://www.medworm.com/index.php?rid=3017420&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926588%26dopt%3DAbstract Authors: Hoda SA, Ross D PMID: 19926588 [PubMed - as supplied by publisher] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017420 Mon, 23 Nov 2009 04:50:11 +0100 3017420 http://www.medworm.com/index.php?rid=3017419&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926589%26dopt%3DAbstract Authors: Dimenstein IB, Zarbo RJ PMID: 19926589 [PubMed - as supplied by publisher] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017419 Mon, 23 Nov 2009 04:50:08 +0100 3017419 http://www.medworm.com/index.php?rid=3017418&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19926590%26dopt%3DAbstract Authors: Ankarberg-Lindgren C, Norjavaara E PMID: 19926590 [PubMed - as supplied by publisher] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=3017418 Mon, 23 Nov 2009 04:50:05 +0100 3017418 http://www.medworm.com/index.php?rid=2922452&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846804%26dopt%3DAbstract Authors: Stanley MW PMID: 19846804 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922452 Sat, 24 Oct 2009 14:01:10 +0100 2922452 http://www.medworm.com/index.php?rid=2922451&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846805%26dopt%3DAbstract Authors: Cibas ES, Ali SZ To address terminology and other issues related to thyroid fine-needle aspiration (FNA), the National Cancer Institute (NCI) hosted the NCI Thyroid FNA State of the Science Conference. The conclusions regarding terminology and morphologic criteria from the NCI meeting led to the Bethesda Thyroid Atlas Project and form the framework for The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC). For clarity of communication, TBSRTC recommends that each report begin with 1 of 6 general diagnostic categories. The project participants hope that the adoption of this flexible framework will facilitate communication among cytopathologists, endocrinologists, surgeons, radiologists, and other health care providers; facilitate cytologic-histologic correlation for ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922451 Sat, 24 Oct 2009 14:01:07 +0100 2922451 http://www.medworm.com/index.php?rid=2922449&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846806%26dopt%3DAbstract This report sheds further light on the rapid spread of MRSA across Northeastern Ohio, stressing the need for better education in preventive measures and infection control at the level of community and health care settings. PMID: 19846806 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922449 Sat, 24 Oct 2009 14:01:05 +0100 2922449 http://www.medworm.com/index.php?rid=2922448&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846807%26dopt%3DAbstract We report a pilot study comparing the QFT-GIT and TST results for screening health care workers (HCWs) at Berkshire Medical Center (BMC; Pittsfield, MA), the second hospital in Massachusetts to use QFT-GIT. For the study, 40 BMC HCWs, 20 TST+ and 20 TST-, were screened with the QFT-GIT test. All 20 TST- subjects were also QFT-GIT-, while only 10 of 20 TST+ subjects were QFT-GIT+. The overall agreement between the QFT-GIT and TST results was 75% (kappa = 0.5; 95% confidence interval, 0.268-0.732). The suboptimal agreement was partially due to a higher specificity of QFT-GIT. Confounding factors (eg, bacille Calmette-Guérin vaccination status and birthplace) are discussed, and literature regarding IGRAs and their comparison with TST is reviewed. PMID: 19846807 [PubMed - in process] ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922448 Sat, 24 Oct 2009 14:01:02 +0100 2922448 http://www.medworm.com/index.php?rid=2922447&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846808%26dopt%3DAbstract The objective of this study was to assess differences in semiquantitative PCR values, based on the crossing threshold (Ct) value for cases of HSV meningitis and encephalitis. A retrospective chart review was performed for 29 patients with a documented diagnosis of HSV meningitis or encephalitis during the course of 5 years from a large urban academic hospital. Demographic information, cerebrospinal fluid (CSF) laboratory findings, and PCR data were obtained for each patient. There was no statistically significant difference in the Ct values in patients with meningitis or encephalitis. In addition, no differences were found in CSF chemistry and hematology parameters. There is a broad range in relative concentrations of HSV DNA in the CSF in cases of meningitis and encephalitis. PMID: 19... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922447 Sat, 24 Oct 2009 14:00:59 +0100 2922447 http://www.medworm.com/index.php?rid=2922446&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846809%26dopt%3DAbstract Authors: Muzzafar T, Medeiros LJ, Wang SA, Brahmandam A, Thomas DA, Jorgensen JL We studied CD81 expression by flow cytometry (FC) on benign precursor B cells (hematogones) and leukemic blasts in precursor B-cell acute lymphoblastic leukemia (pre-B-ALL) and established its usefulness in minimal residual disease (MRD) assays. Hematogones showed uniformly bright CD81 expression. In 98 pre-B-ALLs at diagnosis or overt relapse, 80 (82%) showed aberrantly decreased CD81 intensity. We used hematogones in 139 MRD- specimens to set a lower threshold for normal CD81 expression. In 133 specimens positive for residual pre-B-ALL, 87.2% showed increased CD81-dim immature B cells (>10%) and/or a discrete cluster of CD81-dim cells in a background of hematogones. Only 1 of 139 MRD- specimens showed... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922446 Sat, 24 Oct 2009 14:00:57 +0100 2922446 http://www.medworm.com/index.php?rid=2922445&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846810%26dopt%3DAbstract In this study, we evaluated 52 patients with confirmed diagnoses of MCL and identified variant immunophenotypes in 21 patients (19/48 classical and 2/4 variant MCLs), including CD5- in 6 (12%) of 52, CD10+ in 4 (8%) of 50, CD23+ in 10 (21%) of 48, and FMC7- in 4 (11%) of 37 cases. Three cases showed variations in 2 antigens, including CD5-/CD23+, CD10+/FMC7-, and CD23+/FMC7-; they were all classical MCLs. One blastoid variant MCL was CD23+, and one was FMC7-. Evaluation for proliferation index by immunohistochemical analysis for Ki-67 demonstrated no significant difference between MCLs with variant immunophenotypes and MCLs with typical immunophenotypes. The high proliferation index (>60%) was exclusively seen in the blastoid and pleomorphic variants. Our results indicate that immunophe... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922445 Sat, 24 Oct 2009 14:00:54 +0100 2922445 http://www.medworm.com/index.php?rid=2922444&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846811%26dopt%3DAbstract We report 3 cases of lymphocyte-poor thymic remnants identified in central cervical (level VI) lymph node dissections for unrelated benign and malignant pathology. In each, the rests were nearly completely composed of bland epithelial cells with rare admixed lymphocytes. These cells were immunophenotypically similar to thymic epithelial cells, although they differed in the paucity of associated thymic lymphocytes and lack of Hassall corpuscles. Lymphocyte-depleted thymic remnants in the central cervical compartment are not well described in the literature. It is important to be aware of these and other benign inclusions to avoid making incorrect diagnoses of malignancy. PMID: 19846811 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922444 Sat, 24 Oct 2009 14:00:52 +0100 2922444 http://www.medworm.com/index.php?rid=2922443&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846812%26dopt%3DAbstract Authors: Cankovic M, Whiteley L, Hawley RC, Zarbo RJ, Chitale D The presence of the JAK2 V617F mutation is now part of clinical diagnostic algorithms, and JAK2 status is routinely assessed when BCR/ABL- chronic myeloproliferative neoplasms (MPNs) are suspected. The aim of this study was to evaluate performance of 3 screening and 1 quantitative method for JAK2 V617F detection. For the study, 43 samples (27 bone marrow aspirates and 16 peripheral blood samples) were selected. The screening assays were the JAK2 Activating Mutation Assay (InVivoScribe, San Diego, CA), JAK2 MutaScreen kit (Ipsogen, Luminy Biotech, Marseille, France), and a home-brew melting curve analysis method. Ipsogen's JAK2 MutaQuant assay was used for quantification of mutant and wild-type alleles. The limit of detecti... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922443 Sat, 24 Oct 2009 14:00:50 +0100 2922443 http://www.medworm.com/index.php?rid=2922442&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846813%26dopt%3DAbstract We examined the immunoreactivity for CD30 and T-cell receptor (TCR) gamma gene rearrangement in 37 TUGSE cases. Clonal TCR gene rearrangements were demonstrated in 7 (24%) of 29 cases with amplifiable DNA, and the morphologic features and CD30 immunoreactivity of these cases did not differ from those with polyclonal TCR gene rearrangements. Clinical follow-up was available for 5 of 7 TUGSE cases with clonal TCR gene rearrangement for an average period of 1.75 years after the initial biopsy or excision, and there was no evidence of local recurrence or development of systemic T-cell lymphoproliferative disorder. Without morphologic and/or clinical evidence of lymphoma, T-cell clonality and/or CD30 positivity in these lesions is not indicative of malignancy and should be interpreted with caut... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922442 Sat, 24 Oct 2009 14:00:47 +0100 2922442 http://www.medworm.com/index.php?rid=2922441&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846814%26dopt%3DAbstract Authors: Gupta R, Bhaskar A, Kumar L, Sharma A, Jain P Presence of normal plasma cells (PCs), hemodilution of bone marrow aspirate, and changes in the immunophenotype are important considerations in minimal residual disease (MRD) assessment in multiple myeloma (MM). We evaluated 124 subjects-107 with MM, 11 with Hodgkin lymphoma, and 6 allogeneic stem cell transplantation donors-for the immunophenotype of neoplastic, reactive, and normal PCs respectively. Of the patients with MM, 36 were evaluated for MRD and 23 for a change in immunophenotype after chemotherapy. The immunophenotype of normal and reactive PCs was similar and differed from that of neoplastic PCs with respect to CD19, CD45, CD56, CD52, CD20, and CD117. At least 2 antigens were aberrantly expressed in all cases and 3 in 9... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922441 Sat, 24 Oct 2009 14:00:44 +0100 2922441 http://www.medworm.com/index.php?rid=2922440&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846815%26dopt%3DAbstract Authors: Fuda FS, Karandikar NJ, Chen W B-cell maturation from hematogones to mature B cells in bone marrow exhibits a consistent, complex spectrum of sequential antigen expression. CD5 expression, however, has not been characterized. We studied the dynamics of CD5 expression on developing B cells by 4-color flow cytometry in 32 patients, aged 9 months to 63 years, with hematogone hyperplasia (>3.5% of total events). The mean percentage of hematogones was 8.1%. We demonstrate consistent CD5 expression on normal, polytypic B cells in a continuum, predominantly at later stages of maturation, specifically on stage 3 hematogones and mature B cells. Awareness of this normal pattern of CD5 expression on B-cell subsets has implications in the analysis of minimal residual disease of CD5+ B-... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922440 Sat, 24 Oct 2009 14:00:42 +0100 2922440 http://www.medworm.com/index.php?rid=2922439&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846816%26dopt%3DAbstract We describe the cytologic features of chondromyxoid fibroma in fine-needle aspiration biopsy specimens in 4 patients. We analyzed rapid Romanowsky- and Papanicolaou-stained slides with a respect to overall cellularity, the presence of hypercellular chondromyxoid fragments (HCFs), nuclear atypia, cytoplasmic features, and the presence of giant cells. The most consistent and diagnostically useful feature was the presence of HCFs, which were present in all 4 cases. Mild to moderate nuclear atypia was additionally present in all 4 cases. Most cases were hypocellular (3/4). Giant cells were present in 2 cases, with 1 case exhibiting a hypercellular smear with numerous giant cells. All 4 cases were confirmed in subsequent histologic biopsy material. Strict attention to clinical, radiographic, an... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922439 Sat, 24 Oct 2009 14:00:39 +0100 2922439 http://www.medworm.com/index.php?rid=2922438&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846817%26dopt%3DAbstract Authors: Alsharif M, Cameron SE, Young JA, Savik K, Henriksen JC, Gulbahce HE, Pambuccian SE Infectious complications remain an important cause of death in hematopoietic stem cell transplant (HSCT) recipients. We undertook a 20-year (1988-2007) retrospective review of all autopsies performed on HSCT recipients in our institution, with emphasis on infections, especially fungal infections, as the cause of death. Of the 2,943 autopsies performed in our institution from 1988 to 2007, 395 (13.4%) involved HSCT recipients (117 pediatric; 278 adult). Of the patients, 298 had received allogeneic, 46 autologous, 41 umbilical cord blood, and 3 autologous plus allogeneic types, and 7 were unknown HSCT types. The most common causes of death were pulmonary complications, occurring in 247 (62.5%) of... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922438 Sat, 24 Oct 2009 14:00:33 +0100 2922438 http://www.medworm.com/index.php?rid=2922437&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846818%26dopt%3DAbstract We present the first evidence of annexin-V expression in ovarian carcinoma effusions. The higher annexin-V expression compared with other apoptosis parameters and its association with high-grade disease and poor survival in postchemotherapy patients suggest a role in cell survival rather than apoptosis in effusions. PMID: 19846818 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922437 Sat, 24 Oct 2009 14:00:30 +0100 2922437 http://www.medworm.com/index.php?rid=2922436&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846819%26dopt%3DAbstract Authors: Renshaw MA, Renshaw AA, Gould EW For interlaboratory second opinions requested by patients or clinicians, whether all or selected slides should be sent is unknown. We compared the disagreement rate for requested second opinions for 4 years of sending selected slides with the rate for 1 year of sending all slides. Disagreements were identified in 81 (13.6%) of 596 cases (468 selected slides, 128 all slides). The disagreement rate was less when sending selected slides (n = 58 [12%]) vs all slides (n = 23 [18%]; P = .03). Only 5 cases were identified with disagreement related to whether all or selected slides were sent (selected slides, 1 case [slide of interest inadvertently not chosen]; all slides, 4 cases; P = .03). When all slides were sent (disagreement in number of positive... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922436 Sat, 24 Oct 2009 14:00:27 +0100 2922436 http://www.medworm.com/index.php?rid=2922435&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846820%26dopt%3DAbstract Authors: Wilbur DC, Black-Schaffer WS, Luff RD, Abraham KP, Kemper C, Molina JT, Tench WD Location-guided screening in cervical cytology offers a potentially significant advance over routine manual screening. A prospective, 2-armed, masked clinical trial of the BD FocalPoint GS Imaging System using SurePath slides (BD Diagnostics-TriPath, Burlington, NC) compared routine manual screening and quality control rescreening with computer-assisted, field-of-view screening and device-directed quality control rescreening. The results obtained in the 2 arms were compared with adjudicated reference diagnoses for each slide. Sensitivity, specificity, and negative predictive value were calculated for the detection of atypical squamous cells of undetermined significance and greater (ASC-US+), low-g... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922435 Sat, 24 Oct 2009 14:00:24 +0100 2922435 http://www.medworm.com/index.php?rid=2922434&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846821%26dopt%3DAbstract Authors: Nakazawa K, Murata S, Yuminamochi T, Ishii Y, Ohno S, Nakazawa T, Kondo T, Katoh R We immunochemically studied p16(INK4a) expression in 116 urine cytologic samples and compared results with 190 histologic samples. The cytologic samples were classified into 4 groups: 1, mild cellular atypia; 2, moderate cellular atypia; 3, severe cellular atypia; and 4, malignancy. Overexpression of p16(INK4a) was detected in none of 32 cases in group 1, 8 (16%) of 50 cases in group 2, 5 (42%) of 12 cases in group 3, and 11 (50%) of 22 cases in group 4. In addition, by histologic analysis, p16(INK4a) overexpression was not detected in nonneoplastic urothelium, except for a few cases of reactive atypia, but it was detected in about 50% of urothelial carcinomas. In particular, a high incidence (1... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922434 Sat, 24 Oct 2009 14:00:21 +0100 2922434 http://www.medworm.com/index.php?rid=2922433&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846822%26dopt%3DAbstract Authors: Brimo F, Vollmer RT, Case B, Aprikian A, Kassouf W, Auger M The "atypical urothelial cell" cytologic category is nonstandardized. We subclassify atypical cases to "atypical, favor a reactive process" or "atypical, unclear if reactive or neoplastic." We evaluated the predictive significance of atypical cases by looking at their histologic follow-up. Among the 1,114 patients and 3,261 specimens included, 282 specimens had histologic follow-up. An atypical diagnosis did not carry a significant increased risk of urothelial neoplasia compared with the benign category. Although an "atypical unclear" diagnosis carried a higher rate of detection of high-grade cancer on follow-up biopsy in comparison with "atypical reactive" or "negative" diagnoses (26/58 [45%] vs 15/52 [29%] and 16/10... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922433 Sat, 24 Oct 2009 14:00:18 +0100 2922433 http://www.medworm.com/index.php?rid=2922432&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846823%26dopt%3DAbstract We report a case of embolization of hydrophilic coating of a central venous catheter to the lung that resulted in cavitary lung nodules in a 34-year-old woman. The microscopic features of this unusual complication warrant emphasis so that pathologists will not overlook the embolic foreign material and make a faulty diagnosis of noniatrogenic granulomatous vasculitis. PMID: 19846823 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922432 Sat, 24 Oct 2009 14:00:15 +0100 2922432 http://www.medworm.com/index.php?rid=2922431&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846825%26dopt%3DAbstract Authors: Braun S, von Beckerath N, Ellert J, Kastrati A, Schömig A, Vogt W, Sibbing D, Paniccia R, Gori AM, Marcucci R, Prisco D, Abbate R, Antonucci E, Maggini N, Romano E PMID: 19846825 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922431 Sat, 24 Oct 2009 14:00:12 +0100 2922431 http://www.medworm.com/index.php?rid=2922430&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846826%26dopt%3DAbstract Authors: Lee WS, Kim TY PMID: 19846826 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922430 Sat, 24 Oct 2009 14:00:09 +0100 2922430 http://www.medworm.com/index.php?rid=2922429&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19846827%26dopt%3DAbstract Authors: Fiorucci G PMID: 19846827 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2922429 Sat, 24 Oct 2009 14:00:06 +0100 2922429 http://www.medworm.com/index.php?rid=2812804&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762525%26dopt%3DAbstract This study supports the presumed vascular etiology of DH, confirms the rarity of the lesion, and supports the suggestion that it is usually accompanied by other end-organ damage. PMID: 19762525 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812804 Mon, 21 Sep 2009 05:12:48 +0100 2812804 http://www.medworm.com/index.php?rid=2812803&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762526%26dopt%3DAbstract Authors: Wagner PL, Perner S, Rickman DS, LaFargue CJ, Kitabayashi N, Johnstone SF, Weir BA, Meyerson M, Altorki NK, Rubin MA Recent advances in the characterization of the lung cancer genome have suggested that KRAS may frequently be amplified, although little is known regarding the significance of this finding. This is in contrast with activating mutations of KRAS, which occur in approximately 20% of non-small cell lung carcinomas (NSCLCs). We used fluorescence in situ hybridization to provide direct evidence of KRAS amplification for the first time in clinical specimens. We detected amplification in 7 of 100 consecutive NSCLCs, with a concurrent activating KRAS mutation in 4 cases. KRAS amplification was associated with greater expression of p21 as assessed by quantitative immunohis... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812803 Mon, 21 Sep 2009 05:12:44 +0100 2812803 http://www.medworm.com/index.php?rid=2812802&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762527%26dopt%3DAbstract Authors: Bellizzi AM, Kahaleh M, Stelow EB Endoscopic ampullectomy (EA) is increasingly used in the management of ampullary neoplasia. Although studies on the safety and efficacy of this procedure exist, no study has specifically addressed the histopathologic features of the specimens. We review our experience with 45 EA specimens assessed for the following: diagnosis, high-grade dysplasia (HGD), submucosal ampullary gland/ductule involvement, specimen integrity, and margin status. Familial adenomatous polyposis (FAP) status and the endoscopist's impression of completeness of removal were also ascertained. Previous biopsy diagnoses were compared with ampullectomy diagnoses, and histologic and clinical features were correlated with disease persistence. The histologic features of the amp... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812802 Mon, 21 Sep 2009 05:12:41 +0100 2812802 http://www.medworm.com/index.php?rid=2812801&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762528%26dopt%3DAbstract Authors: Bartlett JM, Campbell FM, Ibrahim M, Wencyk P, Ellis I, Kay E, Connolly Y, O'Grady A, Di Palma S, Starczynski J, Morgan JM, Jasani B, Miller K Our purposes were to perform a robust assessment of a new HER2 chromogenic in situ hybridization test and report on concordance of silver in situ hybridization (SISH) data with fluorescence in situ hybridization (FISH) data and on intraobserver and interlaboratory scoring consistency. HER2 results were scored from 45 breast cancers in 7 laboratories using the Ventana (Tucson, AZ) INFORM HER-2 SISH assay and in 1 central laboratory using a standard FISH assay. Overall, 94.8% of cases were successfully analyzed by SISH across the 6 participating laboratories that reported data. Concordance for diagnosis of HER2 amplification by SISH compa... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812801 Mon, 21 Sep 2009 05:12:38 +0100 2812801 http://www.medworm.com/index.php?rid=2812800&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762529%26dopt%3DAbstract We report our experience with synoptic reporting on head and neck neoplasms, demonstrating, in particular, how this can be customized according to needs of each institution. PMID: 19762529 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812800 Mon, 21 Sep 2009 05:12:35 +0100 2812800 http://www.medworm.com/index.php?rid=2812799&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762530%26dopt%3DAbstract Authors: Saad RS, Ismiil N, Dubé V, Nofech-Mozes S, Khalifa MA We studied the expression of cytokeratin (CK) 7, CK20, CDX-2, and p16 in 119 cervical adenocarcinomas (65 usual type [50 invasive; 15 in situ], 37 intestinal type [21 invasive; 16 in situ], 10 endometrioid, 5 adenosquamous, and 2 signet-ring carcinomas) in comparison with 55 cases of rectal adenocarcinomas. The percentage of cells staining was considered negative if 0% to 5% stained; more than 5% was considered positive. For p16, staining of more than 50% was considered positive. CK7 was expressed in all cervical cases and in 12 rectal adenocarcinomas (22%). CK20 was expressed in 17 cervical adenocarcinomas (14.3%) and in 48 rectal adenocarcinomas (87%). CK20 immunostaining was diffuse in the majority of rectal tumors ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812799 Mon, 21 Sep 2009 05:12:33 +0100 2812799 http://www.medworm.com/index.php?rid=2812798&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762531%26dopt%3DAbstract Authors: Penault-Llorca F, Bilous M, Dowsett M, Hanna W, Osamura RY, Rüschoff J, van de Vijver M Patients with human epidermal growth factor receptor-2 (HER2)+ breast cancer are eligible for trastuzumab treatment; therefore, accurate assessment of HER2 status is essential. Until recently, only 2 methods were validated for determining the HER2 status of breast tumors in the routine diagnostic setting: immunohistochemical analysis and fluorescence in situ hybridization (FISH). Recently, bright-field in situ hybridization techniques such as chromogenic in situ hybridization (CISH) and silver-enhanced in situ hybridization (SISH), which combine features of immunohistochemical analysis and FISH, have been introduced for the determination of HER2 status. These new techniques use a perox... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812798 Mon, 21 Sep 2009 05:12:30 +0100 2812798 http://www.medworm.com/index.php?rid=2812797&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762532%26dopt%3DAbstract Authors: Delost MD, Miller WG, Chang GA, Korzun WJ, Nadder TS The influence of the training, experience, and credentials of testing personnel in the increasingly complex laboratory is a significant concern. A retrospective study compared the relationship of proficiency testing (PT) performance with the credentials of the testing personnel using logistic regression analyses. Predictor variables included the practitioner's college major, degree, certification, and years of laboratory experience. There were 14,326 valid PT results and 359 practitioners of whom 245 (68.2%) had a clinical laboratory science major. Logistic regression analysis revealed that participants without a clinical laboratory science major were more likely (adjusted odds ratio, 1.849; P = .035) to produce unacceptable... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812797 Mon, 21 Sep 2009 05:12:28 +0100 2812797 http://www.medworm.com/index.php?rid=2812796&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762533%26dopt%3DAbstract Authors: Holland L, Blick K Noninvasive, transcutaneous bilirubin (TcB) measurement is an attractive option for neonates, but opinions about its usefulness vary among studies. We collected paired measurements of TcB and serum bilirubin (SB) in 343 term neonates using the BiliCheck meter (SpectRx, Norcross, GA) and 3 different SB methods. Correlations between SB and TcB were similar for all laboratory methods and TcB measurement sites. However, TcB bias varied depending on the comparison SB method and TcB measurement site. TcB bias also varied with race when measurements were done on the forehead but not when they were done on the sternum. Several factors must be considered before implementing TcB measurement: (1) Each laboratory instrument has its own unique relationship to TcB. (2) Th... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812796 Mon, 21 Sep 2009 05:12:25 +0100 2812796 http://www.medworm.com/index.php?rid=2812795&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762534%26dopt%3DAbstract Authors: Sutherland DR, Kuek N, Azcona-Olivera J, Anderson T, Acton E, Barth D, Keeney M Diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) with flow cytometry traditionally involves the analysis of CD55 and CD59 on RBCs and neutrophils. However, the ability to accurately detect PNH RBCs is compromised by prior hemolysis and/or transfused RBCs. Patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS) can also produce PNH clones. We recently described a multiparameter fluorescent aerolysin (FLAER)-based flow assay using CD45, CD33, and CD14 that accurately identified PNH monocyte and neutrophil clones in PNH, AA, and MDS. Here, we compared the efficiency of this WBC assay with a CD59-based assay on RBCs during a 3-year period. PNH clones were detected with the FLAER ass... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812795 Mon, 21 Sep 2009 05:12:22 +0100 2812795 http://www.medworm.com/index.php?rid=2812794&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762535%26dopt%3DAbstract Authors: Hassanein NM, Alcancia F, Perkinson KR, Buckley PJ, Lagoo AS We compared the expression of CD123, the alpha chain of the interleukin-3 receptor, on normal B-cell precursors in bone marrow ("hematogones") from 75 specimens and on leukemic blasts in 45 newly diagnosed B-acute lymphoblastic leukemias (B-ALL) cases. We found that the less mature hematogones (dim CD45+) that express CD34 lack CD123 expression, whereas the more mature hematogones (moderate CD45+) lack CD34 but always express CD123. In contrast with this discordant pattern of CD34 and CD123 expression in hematogones, blasts in 41 (91%) of 45 cases of B-ALL showed concordant expression of the 2 antigens: 80% (36 of 45) cases expressed both antigens, whereas 11% (5 of 45) expressed neither. We found that these distinct... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812794 Mon, 21 Sep 2009 05:12:20 +0100 2812794 http://www.medworm.com/index.php?rid=2812793&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762536%26dopt%3DAbstract This report describes the clinical, histologic, phenotypic, and genotypic findings in 4 cases of plasmacytoma-like PTLD (2 nodal, 1 adenoidal, and 1 cutaneous) and compares the findings with extramedullary involvement by plasma cell neoplasms arising in immunocompetent patients. Plasmacytoma-like PTLDs characteristically arise late after transplantation (mean, 7.0 years), show a variable association with Epstein-Barr virus (2/4 cases positive), and demonstrate histologic and phenotypic findings that overlap with immunocompetent extramedullary plasma cell neoplasms. None of the patients with plasmacytoma-like PTLD developed lytic bone lesions, and 3 of 4 patients had complete responses (>2 years) to reduction of immunosuppression, confirming the role of immunosuppression in the pathogene... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812793 Mon, 21 Sep 2009 05:12:17 +0100 2812793 http://www.medworm.com/index.php?rid=2812792&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762537%26dopt%3DAbstract Authors: M'Hidi H, Thibult ML, Chetaille B, Rey F, Bouadallah R, Nicollas R, Olive D, Xerri L B- and T-lymphocyte attenuator (BTLA) is a lymphoid receptor that inhibits lymphocyte activation on interaction with its ligand, herpesvirus entry mediator (HVEM). We developed monoclonal antibodies against BTLA and HVEM to study their expression using immunohistochemical and flow cytometric analyses in human tissues. In reactive lymph nodes, they were both expressed in interfollicular T cells and in B cells from mantle and marginal zones. Within germinal centers, B cells were negative, whereas T follicular helper (TFH) cells were BTLA+ and follicular dendritic cells were HVEM+. BTLA was strongly expressed in chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL, 19 of 19 positive... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812792 Mon, 21 Sep 2009 05:12:14 +0100 2812792 http://www.medworm.com/index.php?rid=2812791&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762538%26dopt%3DAbstract This study brings the total number of reported cases in the literature to 6. All patients were HIV+ with very low CD4 counts at diagnosis. The involved locations were mediastinum, anus, and bone marrow. Tumors exhibited predominantly immunoblastic/plasmablastic morphologic features and had a plasma cell-like immunophenotype. Bright CD38 expression by flow cytometry had a tendency to be more common in these cases compared with PBL without MYC rearrangement. All cases were positive for Epstein-Barr virus-encoded RNA but lacked human herpesvirus-8 latent nuclear antigen. The 2 patients with follow-up died within 3 months. These findings show that PBL is often associated with MYC/IgH rearrangements and that this finding may portend an aggressive clinical course, suggesting that cytogenetic stu... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812791 Mon, 21 Sep 2009 05:12:12 +0100 2812791 http://www.medworm.com/index.php?rid=2812790&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762539%26dopt%3DAbstract Authors: Sanfelippo MJ, Tillema VB An assay for fondaparinux (Arixtra) is described based on a modified commercial assay for heparin. The assay is automated on a Dade Behring BCS XP (Siemens Healthcare Diagnostics, Deerfield, IL) and uses the inhibition of activated factor X to quantitate the drug. The assay was unaffected by platelet contamination or the presence of warfarin. The assay was affected by the antithrombin level, and the value obtained in the assay decreased significantly when the antithrombin level was less than 60%. The assay is, however, not specific for fondaparinux. Specimens containing unfractionated or low-molecular-weight heparin will yield results by this assay that will not be an accurate estimation of concentration. PMID: 19762539 [PubMed - in process] (Sour... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812790 Mon, 21 Sep 2009 05:12:10 +0100 2812790 http://www.medworm.com/index.php?rid=2812789&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762540%26dopt%3DAbstract Authors: Greco TP, Conti-Kelly AM, Greco T, Doyle R, Matsuura E, Anthony JR, Lopez LR Antiphospholipid antibodies (aPLs) have been implicated in atherogenesis. We studied 344 patients with acute coronary syndromes; approximately 40% were aPL+ in 1 or more tests and 60% aPL-. In 215 patients, coronary artery disease (CAD) was angiographically documented, with 43.7% positive for aPL vs 34.9% of patients without CAD positive for aPLs. Anti-beta(2)-glycoprotein I (beta2GPI; 54%) and anti-oxidized low-density lipoprotein (oxLDL)/beta2GPI (48%) were most frequent, accounting for 87% of all aPL+ CAD cases. aPLs correlated with severity of CAD (P = .012). Adverse events occurred in 16.7% of patients with CAD, more frequently in patients who were aPL+ (P = .0006; relative risk, 2.9; 95% confide... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812789 Mon, 21 Sep 2009 05:12:07 +0100 2812789 http://www.medworm.com/index.php?rid=2812788&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19762541%26dopt%3DAbstract Authors: Gibbs G PMID: 19762541 [PubMed - in process] (Source: American Journal of Clinical Pathology) American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2812788 Mon, 21 Sep 2009 05:12:05 +0100 2812788 http://www.medworm.com/index.php?rid=2716989&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19687307%26dopt%3DAbstract Authors: Lee-Lewandrowski E, Nichols J, Van Cott E, Grisson R, Louissaint A, Benzer T, Lewandrowski K Overcrowding and prolonged patient length-of-stay (LOS) in emergency departments (EDs) are growing problems. We evaluated the impact of implementing a rapid whole blood quantitative D-dimer test (Biosite Triage, Biosite Diagnostics, San Diego, CA) in our ED satellite laboratory on 252 patients before vs 211 patients after implementation. All patients also underwent testing with the existing central laboratory method (VIDAS D-dimer, bioMérieux, Durham, NC). D-dimer turnaround time (from blood draw to result) decreased approximately 79% (approximately 2 hours vs 25 minutes). The mean ED LOS declined from 8.46 to 7.14 hours (P = .016). Hospital admissions decreased 13.8%, ED discharg... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2716989 Thu, 20 Aug 2009 23:52:36 +0100 2716989 http://www.medworm.com/index.php?rid=2716988&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19687308%26dopt%3DAbstract Authors: García-Alcalá H, Ruiz-Argüelles A, Cedillo-Carvallo B Our objective was to compare in a prospective study the clinical performance of the reference and an alternative method to measure blood levels of glycated hemoglobin. A total of 178 samples from patients with diabetes were tested by both methods, and results were analyzed for correlation and comparison of sensitivity, specificity, and positive and negative predictive values to classify patients according to glycemic control. There was a significant linear correlation between methods (r = 0.645; P < .0001); the sensitivity, specificity, and positive and negative predictive values of the alternative method to identify patients with controlled and uncontrolled status were as follows: controlled, 88%, 78%, 77... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2716988 Thu, 20 Aug 2009 23:52:34 +0100 2716988 http://www.medworm.com/index.php?rid=2716987&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19687309%26dopt%3DAbstract Authors: Siegel AJ, d'Hemecourt P, Adner MM, Shirey T, Brown JL, Lewandrowski KB Dysnatremia may cause life-threatening encephalopathy in marathon runners. Hypernatremia and exercise-associated hyponatremia (EAH) may manifest with mental status changes and, if untreated, progress to coma and death. We reviewed the on-site blood sodium testing and treatment in collapsed runners at the finish-line medical tent at the Boston marathons from 2001 through 2008. Dysnatremia was diagnosed in 429 (32.5%) of 1,319 collapsed runners. Hypernatremia was present in 366 (27.7%) and hyponatremia in 63 (4.8%). Hypernatremic runners unable to drink fluids were treated with intravenous normal (0.9%) saline. Hyponatremic runners with seizures or coma received intravenous hypertonic (3%) saline. Sixteen ru... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2716987 Thu, 20 Aug 2009 23:52:31 +0100 2716987 http://www.medworm.com/index.php?rid=2716986&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19687310%26dopt%3DAbstract We report 2 cases of splenic postchemotherapy histiocyte-rich pseudotumor. Each patient had a history of diffuse large B-cell lymphoma, treated with multiagent chemotherapy. Computed tomography scans performed on both patients showed splenic masses. A positron emission tomography scan performed on 1 patient showed increased metabolic activity. The preoperative diagnosis in both patients was recurrent lymphoma, prompting splenectomy. The splenectomy specimens showed multiple, tan-white, firm nodules, up to 3.5 cm in diameter, that were histologically composed of central necrotic B cells (CD20+/CD3-), consistent with necrotic lymphoma, surrounded by numerous lipid-laden (xanthomatous) histiocytes. Clinical staging studies at the time of splenectomy showed no other sites of disease. We conclu... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2716986 Thu, 20 Aug 2009 23:52:27 +0100 2716986 http://www.medworm.com/index.php?rid=2716985&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19687311%26dopt%3DAbstract We present validation data for the Agilent Human Genome Microarray Kit 244K for clinical application. The platform contains approximately 240,000 distinct 60-mer oligonucleotide probes spanning the entire human genome. We studied 45 previously characterized samples (43 abnormal, 2 normal), 32 with knowledge of prior results and 13 in a blinded manner with 11 performed in a reference laboratory providing microarray testing. Array analysis confirmed known aberrations in 43 samples and a normal result in 2. The array analysis corrected 1 karyotype and clarified 2 additional cases. Array data from 6 patients with 22q11.2 deletion found an average of 2.56 megabases (Mb; range, 2.49-2.62 Mb) with a common 2.43-Mb deleted region. Approximately 7 copy number variants from 400 base pairs to 1.6 Mb ... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2716985 Thu, 20 Aug 2009 23:52:25 +0100 2716985 http://www.medworm.com/index.php?rid=2716984&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19687312%26dopt%3DAbstract Authors: Natkunam Y, Tedoldi S, Paterson JC, Zhao S, Rodriguez-Justo M, Beck AH, Siebert R, Mason DY, Marafioti T c-Maf, a leucine zipper-containing transcription factor, is involved in the t(14;16)(q32;q23) translocation found in 5% of myelomas. A causal role for c-Maf in myeloma pathogenesis has been proposed, but data on c-Maf protein expression are lacking. We therefore studied the expression of c-Maf protein by immunohistochemical analysis in myelomas and in a wide variety of hematopoietic tissue. c-Maf protein was detected in a small minority (4.3%) of myelomas, including a t(14;16)(q32;q22-23)/IgH-Maf+ case, suggesting that c-Maf protein is not expressed in the absence of c-Maf rearrangement. In contrast, c-Maf was strongly expressed in hairy cell leukemia (4/4) and in a signifi... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2716984 Thu, 20 Aug 2009 23:52:23 +0100 2716984 http://www.medworm.com/index.php?rid=2716983&cid=s_37382_32_f&fid=37382&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19687313%26dopt%3DAbstract Authors: Deshmukh-Rane SA, Wu ML Pseudolipomatosis refers to optically clear vacuoles that artifactually contaminate specimens, and it most commonly affects the gastrointestinal tract. Pseudolipomatosis closely resembles adult white fat and is of group "A" or "B" when vacuoles have mild or marked variation in size, respectively. Pseudolipomatosis has yet to be reported to occur in the endometrium. Pseudolipomatosis in the endometrium might be easily mistaken for extrauterine adipocytes and misdiagnosed as perforation. We retrospectively reviewed 50 consecutive specimens from endometrial biopsies to determine the prevalence of endometrial pseudolipomatosis and whether pseudolipomatosis was related to perforation. All 50 specimens contained pseudolipomatosis of group "B," and lacked extr... American Journal of Clinical Pathology journals http://www.medworm.com/rss/comments.php?id=2716983 Thu, 20 Aug 2009 23:52:20 +0100 2716983