MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Analytical and Bioanalytical Chemistry' source. Thu, 09 Feb 2012 13:01:26 +0100 http://www.medworm.com/index.php?rid=5654345&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22293971%26dopt%3DAbstract In conclusion, the developed method is capable of determining all regulated mycotoxins in maize and presuming similar matrix effects and extraction recovery also in other cereal-based foods. PMID: 22293971 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654345 Thu, 02 Feb 2012 05:00:00 +0100 5654345 http://www.medworm.com/index.php?rid=5654325&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297855%26dopt%3DAbstract Authors: Burns DT, Bojović S PMID: 22297855 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654325 Thu, 02 Feb 2012 05:00:00 +0100 5654325 http://www.medworm.com/index.php?rid=5654324&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297856%26dopt%3DAbstract Authors: Gonzalo-Lumbreras R, Sanz-Landaluze J, Guinea J, Cámara C Abstract Triclosan, an antibacterial and antifungal agent, is widely used in household and personal care products, processed foods and food packaging, etc., and thus also released into the environment. Triclosan is acutely and chronically toxic to aquatic organisms and bioaccumulates in fish tissue. Here, we propose a new miniaturized triclosan extraction method for aqueous and fish roe samples, based on the use of a vortex mixer and an ultrasonic probe, respectively, and useful for triclosan determination by gas chromatography coupled to a micro electron capture detector. Different solvents for extraction and sorbents for clean-up purposes were tested. Multivariate optimization of the variables affecting ultrasoni... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654324 Thu, 02 Feb 2012 05:00:00 +0100 5654324 http://www.medworm.com/index.php?rid=5654323&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297857%26dopt%3DAbstract Authors: Zhang YP, Shi SY, Xiong X, Chen XQ, Peng MJ Abstract Traditional activity-guided fractionation of natural products is a time-consuming, labor intensive, and expensive strategy, which cannot compete with high-throughput and rapid screening of natural products. Therefore, more efficient approaches are necessary for searching active compounds from natural products. Three main methods based on high-performance liquid chromatography (HPLC) analysis combined with 2,2'-diphenyl-1-picrylhydrazyl (DPPH) assay, DPPH spiking HPLC analysis, on-line post-column HPLC-DPPH analysis, and HPLC-based DPPH activity profiling, were then developed for the rapid screening of antioxidants from complex mixtures. In the present study, a comparative study of these three methods has been conducted t... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654323 Thu, 02 Feb 2012 05:00:00 +0100 5654323 http://www.medworm.com/index.php?rid=5654321&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297858%26dopt%3DAbstract Authors: Wipawakarn P, Ju H, Wong DK Abstract A label-free electrochemical biosensor for detecting DNA hybridisation was developed by monitoring the change in the voltammetric activity of ferrocenecarboxylic acid at the biosensor-solution interface. The biosensor was constructed by initially immobilising on a glassy carbon electrode an anchoring layer consisting of chitosan, carboxyl group functionalised carbon nanofibres and glutaraldehye. Chitosan acted as an adhering agent and carbon nanofibres were strategically used to provide a large surface area with binding points for DNA immobilisation, while glutaraldehye was a linker for DNA probes on the electrode surface. Based on a two-factorial design, cyclic voltammetry of [Fe(CN)(6)](3-/4-) was performed to optimise the composition... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654321 Thu, 02 Feb 2012 05:00:00 +0100 5654321 http://www.medworm.com/index.php?rid=5654318&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297859%26dopt%3DAbstract Authors: Saleh S, Moreno-Molek S, Perera I, Riga A, Sam-Yellowe T, Bayachou M Abstract The antibody specific for the malaria protein, Rhop-3, and FL-Rhop-3, were immobilized on the surface of a gold electrode modified with cysteamine. Colloidal gold was used to enhance the detection signal for Rhop-3 antigens. The Rhop-3 antibody was also immobilized on gold electrodes preactivated with dithiobis(succinimidyl proprionate) (DSP). Immobilization was performed at room temperature and at 37 °C. Cyclic voltammetry (CV) was used to monitor the interaction between the immobilized antibody and its cognate antigen in solution, using ferricyanide, K(3)Fe(CN)(6), as reporting electroactive probe. Tests indicate recognition of Rhop-3 protein by the immobilized antibody. Antigen recognition w... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654318 Thu, 02 Feb 2012 05:00:00 +0100 5654318 http://www.medworm.com/index.php?rid=5654307&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297860%26dopt%3DAbstract Authors: Briones C, Moreno M Abstract Nucleic acid biosensors have a growing number of applications in genetics and biomedicine. This contribution is a critical review of the current state of the art concerning the use of nucleic acid analogues, in particular peptide nucleic acids (PNA) and locked nucleic acids (LNA), for the development of high-performance affinity biosensors. Both PNA and LNA have outstanding affinity for natural nucleic acids, and the destabilizing effect of base mismatches in PNA- or LNA-containing heterodimers is much higher than in double-stranded DNA or RNA. Therefore, PNA- and LNA-based biosensors have unprecedented sensitivity and specificity, with special applicability in DNA genotyping. Herein, the most relevant PNA- and LNA-based biosensors are presente... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654307 Thu, 02 Feb 2012 05:00:00 +0100 5654307 http://www.medworm.com/index.php?rid=5654254&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22297861%26dopt%3DAbstract Authors: Cordero C, Zebelo SA, Gnavi G, Griglione A, Bicchi C, Maffei ME, Rubiolo P PMID: 22297861 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654254 Thu, 02 Feb 2012 05:00:00 +0100 5654254 http://www.medworm.com/index.php?rid=5654347&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22293969%26dopt%3DAbstract Authors: Mallol R, Rodríguez MA, Heras M, Vinaixa M, Plana N, Masana L, Morris GA, Correig X Abstract The sizes of certain types of lipoprotein particles have been associated with an increased risk of cardiovascular disease. However, there is currently no gold standard technique for the determination of this parameter. Here, we propose an analytical procedure to measure lipoprotein particles sizes using diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY). The method was tested on six lipoprotein fractions, VLDL, IDL, LDL(1), LDL(2), HDL(2), and HDL(3), which were obtained by sequential ultracentrifugation from four patients. We performed a pulsed-field gradient experiment on each fraction to obtain a mean diffusion coefficient, and then determined the apparent hydrody... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654347 Wed, 01 Feb 2012 05:00:00 +0100 5654347 http://www.medworm.com/index.php?rid=5654346&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22293970%26dopt%3DAbstract We report herein a new class of metal ion chemosensors and give the first example of a metal-dependent peptidase chemosensor for metal ions. The chemosensor contains the basic specific Ni(II)-dependent peptide bond hydrolysis sequence (Gly-Ala-Ser-Arg-His-Trp-Lys-Phe-Lys). The substrate was labeled with a fluorophore at the N-terminal and a quencher at the C-terminal Lys side chain. Initially, the MOCAc ((7-methoxycoumarin-4-yl)acetyl-) emission was quenched by the nearby quencher. In the presence of Ni(II), the substrate was irreversibly cleaved at the cleavage site, leading to a 20-fold increase in fluorescence intensity. The chemosensor combines the high selectivity of a peptidase (at least greater than tenfold for Ni(II) over other metal ions) with the high sensitivity of fluorescence ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654346 Wed, 01 Feb 2012 05:00:00 +0100 5654346 http://www.medworm.com/index.php?rid=5654389&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22286125%26dopt%3DAbstract Authors: Iparraguirre A, Navarro P, Prieto A, Rodil R, Olivares M, Fernández LA, Zuloaga O Abstract Membrane-assisted solvent extraction coupled to large volume injection in a programmable temperature vaporisation injector using gas chromatography-mass spectrometry analysis was optimised for the simultaneous determination of a variety of endocrine disrupting compounds in environmental water samples (estuarine, river and wastewater). Among the analytes studied, certain hormones, alkylphenols and bisphenol A were included. The nature of membranes, extraction solvent, extraction temperature, solvent volume, extraction time, ionic strength and methanol addition were evaluated during the optimisation of the extraction. Matrix effects during the extraction step were studied in different... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654389 Tue, 31 Jan 2012 05:00:00 +0100 5654389 http://www.medworm.com/index.php?rid=5654356&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290387%26dopt%3DAbstract Authors: Dittrich P, Günther D, Hopfgartner G, Zenobi R PMID: 22290387 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654356 Tue, 31 Jan 2012 05:00:00 +0100 5654356 http://www.medworm.com/index.php?rid=5654353&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290388%26dopt%3DAbstract Authors: Struss AK, Pasini P, Flomenhoft D, Shashidhar H, Daunert S Abstract Quorum sensing (QS) allows bacteria to communicate with one another by means of QS signaling molecules and control certain behaviors in a group-based manner, including pathogenicity and biofilm formation. Bacterial gut microflora may play a role in inflammatory bowel disease pathogenesis, and antibiotics are one of the available therapeutic options for Crohn's disease. In the present study, we employed genetically engineered bioluminescent bacterial whole-cell sensing systems as a tool to evaluate the ability of antibiotics commonly employed in the treatment of chronic inflammatory conditions to interfere with QS. We investigated the effect of ciprofloxacin, metronidazole, and tinidazole on quorum sensing.... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654353 Tue, 31 Jan 2012 05:00:00 +0100 5654353 http://www.medworm.com/index.php?rid=5654350&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22290389%26dopt%3DAbstract Authors: Esteban-Fernández de Ávila B, Pedrero M, Campuzano S, Escamilla-Gómez V, Pingarrón JM Abstract The preparation and characteristics of a disposable amperometric magnetoimmunosensor, based on the use of functionalized magnetic beads (MBs) and gold screen-printed electrodes (Au/SPEs), for the specific detection and quantification of Staphylococcal protein A (ProtA) and Staphylococcus aureus (S. aureus) is reported. An antiProtA antibody was immobilized onto ProtA-modified MBs, and a competitive immunoassay involving ProtA antigen labelled with HRP was performed. The resulting modified MBs were captured by a magnetic field on the surface of tetrathiafulvalene-modified Au/SPEs and the amperometric response obtained at -0.15 V vs the silver pseudo-reference electrode of the... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654350 Tue, 31 Jan 2012 05:00:00 +0100 5654350 http://www.medworm.com/index.php?rid=5654395&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22286077%26dopt%3DAbstract Authors: Phillips MM Abstract Choline is a quaternary amine that is synthesized in the body or consumed through the diet. Choline is critical for cell membrane structure and function and in synthesis of the neurotransmitter acetylcholine. Although the human body produces this micronutrient, dietary supplementation of choline is necessary for good health. The major challenge in the analysis of choline in foods and dietary supplements is in the extraction and/or hydrolysis approach. In many products, choline is present as choline esters, which can be quantitated individually or treated with acid, base, or enzymes in order to release choline ions for analysis. A critical review of approaches based on extraction and quantitation of each choline ester as well as hydrolysis-based methods... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654395 Mon, 30 Jan 2012 05:00:00 +0100 5654395 http://www.medworm.com/index.php?rid=5654393&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22286079%26dopt%3DAbstract Authors: Serafín V, Ubeda N, Agüí L, Yáñez-Sedeño P, Pingarrón JM Abstract In this paper, an electrochemical magneto-immunosensor for the detection of human growth hormone (hGH) is described for the first time. The immunosensor involves the use of tosyl-activated magnetic microparticles (TsMBs) to covalently immobilize a monoclonal mAbhHG antibody. A sandwich-type immunoassay with a secondary pAbhGH antibody and anti-IgG labelled with alkaline phosphatase (anti-IgG-AP) was employed. TsMBs-mAbhGH-hGH-pAbhGH-anti-IgG-AP conjugates were deposited onto the surface of a screen-printed gold electrode using a small neodymium magnet, and electrochemical detection was performed by square-wave voltammetry upon the addition of 4-aminophenyl phosphate as the AP substrate. All the variab... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654393 Mon, 30 Jan 2012 05:00:00 +0100 5654393 http://www.medworm.com/index.php?rid=5654392&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22286080%26dopt%3DAbstract Authors: Shackman JG, Reid KR, Dugan CE, Kennedy RT Abstract A rapid microfluidic based capillary electrophoresis immunoassay (CEIA) was developed for on-line monitoring of glucagon secretion from pancreatic islets of Langerhans. In the device, a cell chamber containing living islets was perfused with buffers containing either high or low glucose concentration. Perfusate was continuously sampled by electroosmosis through a separate channel on the chip. The perfusate was mixed on-line with fluorescein isothiocyanate-labeled glucagon (FITC-glucagon) and monoclonal anti-glucagon antibody. To minimize sample dilution, the on-chip mixing ratio of sampled perfusate to reagents was maximized by allowing reagents to only be added by diffusion. Every 6 s, the reaction mixture was injected ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654392 Mon, 30 Jan 2012 05:00:00 +0100 5654392 http://www.medworm.com/index.php?rid=5654391&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22286081%26dopt%3DAbstract Authors: Abi Jaoudé M, Randon J, Bordes C, Lanteri P, Bois L Abstract A design of experiement approach is described for the optimization of the microscopic morphology of macro-mesoporous titania monoliths that were elaborated for the chromatographic enrichment of phosphorylated compounds. The monolithic titania gels were formed via an alkoxy-derived sol-gel route in association with a phase separation mechanism. The synthesis was performed at mild temperatures of gelation using starting mixtures of titanium n-propoxide, hydrochloric acid, N-methylformamide, water, and poly (ethylene oxide). The gelation temperature and the chemical compositions of N-methylformamide, water, and poly (ethylene oxide) were chosen as the most relevant experimental factors of the sol-gel process. Using... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654391 Mon, 30 Jan 2012 05:00:00 +0100 5654391 http://www.medworm.com/index.php?rid=5654390&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22286082%26dopt%3DAbstract Authors: Martinotti V, Balordi M, Ciceri G Abstract A flow injection analyser coupled with a gas diffusion membrane and a conductometric microdetector was adapted for the field analysis of natural concentrations of free dissolved CO(2) and dissolved inorganic carbon in natural waters and used in a number of field campaigns for marine water monitoring. The dissolved gaseous CO(2) presents naturally, or that generated by acidification of the sample, is separated by diffusion using a hydrophobic semipermeable gas porous membrane, and the permeating gas is incorporated into a stream of deionised water and measured by means of an electrical conductometric microdetector. In order to make the system suitable and easy to use for in-field measurements aboard oceanographic ships, the single ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654390 Mon, 30 Jan 2012 05:00:00 +0100 5654390 http://www.medworm.com/index.php?rid=5654394&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22286078%26dopt%3DAbstract Authors: Carpinteiro I, Abuin B, Ramil M, Rodríguez I, Cela R Abstract An improved selectivity method for the simultaneous determination of four benzotriazoles (benzotriazole, 4-methylbenzotriazole, 5-methylbenzotriazole, and 5,6-dimethyl-1H-benzotriazole) and six benzothiazoles (benzothiazole, 2-hydroxybenzothiazole, 2-benzothiazolamine, mercaptobenzothiazole, 2-methylbenzothiazole, and 2-methylthiobenzothiazole) in aqueous matrices has been developed. Under optimal conditions, analytes are concentrated using a MAX solid-phase extraction (SPE) cartridge, based on divinylbenzene-N-vinylpyrrolidone functionalized with quaternary amine groups, which allows reversed-phase interactions in combination with ionic exchange. Selected compounds are recovered with methanol-acetone 7:3 (v/v)... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654394 Sun, 29 Jan 2012 05:00:00 +0100 5654394 http://www.medworm.com/index.php?rid=5654358&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22287049%26dopt%3DAbstract We present here a variation of this method using amine-derivatized Fe(3)O(4) nanoparticles, in concert with in situ peptide N-glycosidase F digestion for direct matrix-assisted laser desorption/ionization-mass spectrometry analysis of N-glycosylation sites and the released glycans. Conditions were also optimized for efficient elution of the enriched glycopeptides from the nanoparticles for on-line nanoflow liquid chromatography-MS/MS analysis. Successful applications to single glycoproteins as well as total proteomic mixtures derived from biological fluids established the unrivaled practical versatility of this method, with enrichment efficiency comparable to other HILIC-based methods. PMID: 22287049 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654358 Sun, 29 Jan 2012 05:00:00 +0100 5654358 http://www.medworm.com/index.php?rid=5654396&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22281682%26dopt%3DAbstract Authors: Sorrell CD, Serpe MJ Abstract Thermoresponsive microgels have been shown to be an excellent platform for designing sensor materials. Recently, poly (N-isopropylacrylamide)-co-acrylic acid (pNIPAm-co-AAc) microgel based etalon materials have been described as direct sensing materials that can be designed to have a single, unique color. These color tunable materials show immense promise for sensing due to their spectral sensitivity and bright visual color. Here, we describe a proof-of-concept for etalon sensing of glucose. We found that aminophenylboronic acid (APBA)-functionalized pNIPAm-co-AAc microgels in an etalon respond to 3 mg/mL glucose concentrations by red shifting their reflectance peaks by 110 nm up to 150 nm. Additionally, APBA-functionalized pNIPAm-co-AAc mi... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654396 Sat, 28 Jan 2012 05:00:00 +0100 5654396 http://www.medworm.com/index.php?rid=5654363&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22287048%26dopt%3DAbstract We report the development of a surface plasmon resonance sensor based on the silver ion (Ag(+))-induced conformational change of a cytosine-rich, single-stranded DNA for the detection of Ag(+) and cysteine (Cys) in aqueous solutions. In the free state, single-stranded oligonucleotides fold into double-helical structures through the addition of Ag(+) to cytosine-cytosine (C-C) mismatches. However, in the presence of Cys, which competitively binds to Ag(+), the formation of the C-Ag(+)-C assembly is inhibited, resulting in free-state, single-stranded oligonucleotides. To enhance sensitivity, the DNA intercalator, daunorubicin, was employed to achieve signal enhancement. The detection limit for Ag(+) was 10 nM with a measurement range of 50-2,000 nM, and the detection limit for Cys was 50 ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654363 Sat, 28 Jan 2012 05:00:00 +0100 5654363 http://www.medworm.com/index.php?rid=5654357&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22287050%26dopt%3DAbstract Authors: Moravcova E, Vaclavik L, Lacina O, Hrbek V, Riddellova K, Hajslova J Abstract A sensitive and accurate method utilizing ultra-high performance liquid chromatography (U-HPLC) coupled to high resolution mass spectrometry based on orbitrap technology (orbitrapMS) for the analysis of nine 3-chloropropane-1,2-diol (3-MCPD) diesters in vegetable oils was developed. To remove the interfering triacylglycerols that induce strong matrix effects, a clean-up step on silica gel column was used. The quantitative analysis was performed with the use of deuterium-labeled internal standards. The lowest calibration levels estimated for the respective analytes ranged from 2 to 5 μg kg(-1). Good recovery values (89-120%) and repeatability (RSD 5-9%) was obtained at spiking levels of 2 and 1... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654357 Sat, 28 Jan 2012 05:00:00 +0100 5654357 http://www.medworm.com/index.php?rid=5654399&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22281679%26dopt%3DAbstract Authors: Anthemidis AN, Ioannou KI Abstract A novel, automatic on-line sequential injection dispersive liquid-liquid microextraction (SI-DLLME) method, based on 1-hexyl-3-methylimidazolium hexafluorophosphate ([Hmim][PF(6)]) ionic liquid as an extractant solvent was developed and demonstrated for trace thallium determination by flame atomic absorption spectrometry. The ionic liquid was on-line fully dispersed into the aqueous solution in a continuous flow format while the TlBr (4) (-) complex was easily migrated into the fine droplets of the extractant due to the huge contact area of them with the aqueous phase. Furthermore, the extractant was simply retained onto the surface of polyurethane foam packed into a microcolumn. No specific conditi... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654399 Fri, 27 Jan 2012 05:00:00 +0100 5654399 http://www.medworm.com/index.php?rid=5654398&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22281680%26dopt%3DAbstract Authors: Kokaislová A, Matějka P Abstract Surface-enhanced Raman scattering (SERS) spectroscopy and surface-enhanced infrared absorption (SEIRA) spectroscopy are analytical tools suitable for the detection of small amounts of various analytes adsorbed on metal surfaces. During recent years, these two spectroscopic methods have become increasingly important in the investigation of adsorption of biomolecules and pharmaceuticals on nanostructured metal surfaces. In this work, the adsorption of B-group vitamins pyridoxine, nicotinic acid, folic acid and riboflavin at electrochemically prepared gold and silver substrates was investigated using Fourier transform SERS spectroscopy at an excitation wavelength of 1,064 nm. Gold and silver substrates were prepared by cathodic reduction on... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654398 Fri, 27 Jan 2012 05:00:00 +0100 5654398 http://www.medworm.com/index.php?rid=5654397&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22281681%26dopt%3DAbstract In conclusion, the developed sample preparation method coupled with the nano-HPLC-Chip-MS/MS method was able to reveal the presence of drugs in hairs from the drug abusers, with the enhanced sensitivity, compared with the conventional HPLC-MS/MS. PMID: 22281681 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654397 Fri, 27 Jan 2012 05:00:00 +0100 5654397 http://www.medworm.com/index.php?rid=5654409&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22274282%26dopt%3DAbstract Authors: Van Berkel GJ, Kertesz V Abstract A commercially available chip-based infusion nanoelectrospray ionization system was used to ionize metallo alkylporphyrins for mass spectrometric detection and structure elucidation by mass spectrometry. Different ionic forms of model compounds (nickel (II), vanadyl (II), copper (II), and cobalt (II) octaethylporphyrin) were created by using two different types of conductive pipette tips supplied with the device. These pipette tips provide the conductive contact to solution at which the electrolysis process inherent to electrospray takes places in the device. The original unmodified, bare carbon-impregnated plastic pipette tips were exploited to intentionally electrochemically oxidize (ionize) the porphyrins to form molecular radical catio... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654409 Thu, 26 Jan 2012 05:00:00 +0100 5654409 http://www.medworm.com/index.php?rid=5654406&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22274285%26dopt%3DAbstract Authors: Moreira MM, Morais S, Barros AA, Delerue-Matos C, Guido LF Abstract This paper reports a novel application of microwave-assisted extraction (MAE) of polyphenols from brewer's spent grains (BSG). A 2(4) orthogonal composite design was used to obtain the optimal conditions of MAE. The influence of the MAE operational parameters (extraction time, temperature, solvent volume and stirring speed) on the extraction yield of ferulic acid was investigated through response surface methodology. The results showed that the optimal conditions were 15 min extraction time, 100 °C extraction temperature, 20 mL of solvent, and maximum stirring speed. Under these conditions, the yield of ferulic acid was 1.31 ± 0.04% (w/w), which was fivefold higher than that obtained with conventi... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654406 Thu, 26 Jan 2012 05:00:00 +0100 5654406 http://www.medworm.com/index.php?rid=5654405&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22274286%26dopt%3DAbstract Authors: Tep S, Hincapie M, Hancock WS Abstract The development of a general method for the purification and quantitative glycomic analysis of human plasma samples to characterize global glycosylation changes shall be presented. The method involves multiple steps, including the depletion of plasma via multi-affinity chromatography to remove high abundant proteins, the enrichment of the lower abundant glycoproteins via multi-lectin affinity chromatography, the isotopic derivatization of released glycans, and quantitative analysis by MALDI-TOF MS. Isotopic derivatization of glycans is accomplished using the well-established chemistry of reductive amination to derivatize glycans with either a light analog ((12)C anthranilic acid) or a heavy analog ((13)C(7) anthranilic acid), which al... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654405 Thu, 26 Jan 2012 05:00:00 +0100 5654405 http://www.medworm.com/index.php?rid=5654404&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22278071%26dopt%3DAbstract Authors: Shinton S PMID: 22278071 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654404 Thu, 26 Jan 2012 05:00:00 +0100 5654404 http://www.medworm.com/index.php?rid=5654403&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22278072%26dopt%3DAbstract Authors: O'Donoghue MB, Shi X, Fang X, Tan W Abstract Some researchers have questioned whether synthetic aptamers bind as robustly as natural antibodies. To address this issue, we used single-molecule atomic force microscopy to measure the rupture force between a protein and both its aptamer and its antibody. The rupture force on live cell membranes between the aptamer and protein was 46 ± 26 pN; the force with the antibody was 68 ± 33 pN, we conclude that the binding forces are about equal. PMID: 22278072 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654403 Thu, 26 Jan 2012 05:00:00 +0100 5654403 http://www.medworm.com/index.php?rid=5654402&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22278073%26dopt%3DAbstract This article will provide a brief footing in history before reviewing the latest developments in biosensor applications for analysis of food contaminants (January 2007 to December 2010), focusing on the detection of pathogens, toxins, pesticides and veterinary drug residues by biosensors, with emphasis on articles showing data in food matrices. The main areas of development common to these groups of contaminants include multiplexing, the ability to simultaneously analyse a sample for more than one contaminant and portability. Biosensors currently have an important role in food safety; further advances in the technology, reagents and sample handling will surely reinforce this position. PMID: 22278073 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654402 Thu, 26 Jan 2012 05:00:00 +0100 5654402 http://www.medworm.com/index.php?rid=5654401&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22278074%26dopt%3DAbstract Authors: Latzin JM, Schindler BK, Weiss T, Angerer J, Koch HM Abstract The general population is exposed to acrylamide (AA) mainly through food and tobacco smoke. AA is classified as probably carcinogenic to humans. Glycidamide (GA), as the primary oxidative metabolite, was identified to be the ultimate genotoxic agent. This warrants full investigation of the oxidative pathway in AA metabolism and the share of the oxidative compared to the reductive pathway. 2,3-Dihydroxy-propionamide (OH-PA) as the direct hydrolysis product of GA has been shown to be a major urinary oxidative metabolite in human AA metabolism. We developed an analytical method to reliably quantify OH-PA in urine by GC-MS after a multistep procedure including "stripping" on a solid phase material, lyophilization, s... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654401 Thu, 26 Jan 2012 05:00:00 +0100 5654401 http://www.medworm.com/index.php?rid=5654400&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22278075%26dopt%3DAbstract Authors: Correia PR PMID: 22278075 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654400 Thu, 26 Jan 2012 05:00:00 +0100 5654400 http://www.medworm.com/index.php?rid=5654408&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22274283%26dopt%3DAbstract Authors: Menzel M PMID: 22274283 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654408 Wed, 25 Jan 2012 05:00:00 +0100 5654408 http://www.medworm.com/index.php?rid=5654407&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22274284%26dopt%3DAbstract Authors: Capriotti AL, Caracciolo G, Caruso G, Cavaliere C, Pozzi D, Samperi R, Laganà A Abstract A shotgun proteomics approach was used to compare human plasma protein binding capability with cationic liposomes, DNA-cationic lipid complexes (lipoplexes), and lipid-polycation-DNA (LPD) complexes. Nano-high-performance liquid chromatography coupled with a high-resolution LTQ Orbitrap XL mass spectrometer was used to characterize and compare their protein corona. Spectral counting and area under curve methods were used to perform label-free quantification. Substantial qualitative and quantitative differences were found among proteins bound to the three different systems investigated. Protein variety found on lipoplexes and LPD complexes was richer than that found on cationic liposom... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654407 Wed, 25 Jan 2012 05:00:00 +0100 5654407 http://www.medworm.com/index.php?rid=5654412&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22271099%26dopt%3DAbstract Authors: Bouras G, Nakanishi T, Fujita Y, Tsunemi S, Takubo T, Tanigawa N PMID: 22271099 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654412 Sat, 21 Jan 2012 05:00:00 +0100 5654412 http://www.medworm.com/index.php?rid=5654411&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22271100%26dopt%3DAbstract Authors: Carmona P, Molina M, Calero M, Bermejo-Pareja F, Martínez-Martín P, Alvarez I, Toledano A Abstract Peripheral mononuclear leukocytes from Alzheimer's disease (AD) patients were analyzed by infrared spectroscopy and their spectroscopic properties were compared with those from age-matched healthy controls. Two-dimensional correlation analysis of mean spectra measured at various disease stages shows that the protein secondary structure from AD patients involves β-sheet enrichment and carbonyl intensity increase relative to healthy controls. The area percentages of β-sheets, which were obtained by using a peak ratio second-derivative spectral treatment, were used for receiver operating characteristic (ROC) analysis to distinguish between patients with AD and age-matched he... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654411 Sat, 21 Jan 2012 05:00:00 +0100 5654411 http://www.medworm.com/index.php?rid=5654388&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22287047%26dopt%3DAbstract Authors: Chew G, Walczyk T Abstract Despite the importance of stating the measurement uncertainty in chemical analysis, concepts are still not widely applied by the broader scientific community. The Guide to the expression of uncertainty in measurement approves the use of both the partial derivative approach and the Monte Carlo approach. There are two limitations to the partial derivative approach. Firstly, it involves the computation of first-order derivatives of each component of the output quantity. This requires some mathematical skills and can be tedious if the mathematical model is complex. Secondly, it is not able to predict the probability distribution of the output quantity accurately if the input quantities are not normally distributed. Knowledge of the probability distri... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654388 Sat, 21 Jan 2012 05:00:00 +0100 5654388 http://www.medworm.com/index.php?rid=5654410&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22271101%26dopt%3DAbstract Authors: Qian M, Wu L, Zhang H, Xu M, Li R, Wang X, Sun C Abstract A new sensitive multiresidue liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical method for the determination of 16 insect growth regulator (IGR) residues-RH-5849 (1,2-dibenzoyl-1-tert-butylhydrazine), halofenozide, methoxyfenozide, chromafenozide, fufenozide, tebufenozide, diflubenzuron, chlorbenzuron, triflumuron, hexaflumuron, novaluron, lufenuron, teflubenzuron, flucycloxuron, flufenoxuron, and chlorfluazuron-in herbs (Perilla frutescens, flos chrysanthemi, lily bulbs, and ginger) has been developed. After the herbs had been extracted with acetonitrile, a combined graphitized nonporous carbon/aminopropyl (ENVI-Carb/LC-NH(2)) cartridge and a Florisil cartridge were used to clean up the extracts. ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5654410 Fri, 20 Jan 2012 05:00:00 +0100 5654410 http://www.medworm.com/index.php?rid=5618310&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22262050%26dopt%3DAbstract Authors: Sproß J, Sinz A Abstract A poly(glycidyl methacrylate-co-acrylamide-co-ethylene dimethacrylate) monolith and a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith were prepared in fused silica capillaries (100 μm ID) and modified with monomeric avidin using the glutaraldehyde technique. The biotin binding capacity of monolithic affinity columns with immobilized monomeric avidin (MACMAs) was determined by fluorescence spectroscopy using biotin (5-fluorescein) conjugate, as well as biotin- and fluorescein-labeled bovine serum albumin (BSA). The affinity columns were able to bind 16.4 and 3.7 μmol biotin/mL, respectively. Columns prepared using the poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith retained 7.1 mg BSA/mL, almost six times more t... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618310 Fri, 20 Jan 2012 05:00:00 +0100 5618310 http://www.medworm.com/index.php?rid=5618309&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22262051%26dopt%3DAbstract Authors: Longano D, Ditaranto N, Cioffi N, Di Niso F, Sibillano T, Ancona A, Conte A, Del Nobile MA, Sabbatini L, Torsi L Abstract A new type of nanomaterial has been developed as antibacterial additive for food packaging applications. This nanocomposite is composed of copper nanoparticles embedded in polylactic acid, combining the antibacterial properties of copper nanoparticles with the biodegradability of the polymer matrix. Metal nanoparticles have been synthesised by means of laser ablation, a rising and easy route to prepare nanostructures without any capping agent in a liquid environment. As prepared, nanoparticle suspensions have been easily mixed to a polymer solution. The resulting hybrid solutions have been deposited by drop casting, thus obtaining self-standing antibact... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618309 Fri, 20 Jan 2012 05:00:00 +0100 5618309 http://www.medworm.com/index.php?rid=5618312&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22258206%26dopt%3DAbstract Authors: Adornetto G, Volpe G, De Stefano A, Martini S, Gallucci G, Manzoni A, Bernardini S, Mascini M, Moscone D Abstract Coeliac disease (CD) is a gluten-induced autoimmune enteropathy found in genetically susceptible subjects. Because of the high number of undetected cases, rapid and cheaper screening methods are needed. Currently, the CD diagnosis involves the detection of anti-transglutaminase IgA antibodies (anti-tTG IgA) in blood serum through the use of ELISA systems with confirmation by histology of the intestinal mucosa. A new, rapid magneto-electrochemical immunosensor for CD diagnosis has been developed and applied to serum sample analysis. The system uses magnetic beads coated with tTG antigen to detect anti-tTG antibodies in positive serum samples and an alkaline phos... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618312 Thu, 19 Jan 2012 05:00:00 +0100 5618312 http://www.medworm.com/index.php?rid=5618311&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22258207%26dopt%3DAbstract In this study, we have probed the major cobalt, iron, manganese, and nickel-binding proteins in the marine cyanobacterium Synechococcus sp. WH8102 by using two different fractionation approaches combined with peptide mass fingerprinting. For the identification of intact metalloproteins, multidimensional native chromatography was used to fractionate the proteome, followed by inorganic mass spectrometry to identify metal-enriched fractions. This approach led to the detection of nickel superoxide dismutase together with its predicted cofactor. We also explored the utility of immobilized metal affinity chromatography (IMAC) to isolate subpopulations of proteins that display affinity for a particular metal ion. We conclude that low-resolution 2D liquid chromatography is a viable fractionation t... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618311 Thu, 19 Jan 2012 05:00:00 +0100 5618311 http://www.medworm.com/index.php?rid=5618316&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22252653%26dopt%3DAbstract Letter to the Editor regarding "GC-MS with ethyl chloroformate derivatization for comprehensive analysis of metabolites in serum and its application to human uremia" Anal Bioanal Chem. 2012 Jan 18; Authors: Husek P PMID: 22252653 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618316 Wed, 18 Jan 2012 05:00:00 +0100 5618316 http://www.medworm.com/index.php?rid=5618318&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22249417%26dopt%3DAbstract Authors: Almstetter MF, Oefner PJ, Dettmer K Abstract One of the major objectives in metabolomics is the identification of subtle changes in metabolite profiles as affected by genetic or environmental factors. Comprehensive two-dimensional gas chromatography (GC × GC) hyphenated to a fast-acquisition mass spectrometer is a well-established analytical technique to study the composition of complex samples due to its enhanced separation capacity, sensitivity, peak resolution, and reproducibility. This review reports applications of GC × GC to metabolomics studies of sample of different types (biofluid, cells, tissue, bacteria, yeast, plants), and discusses its advantages and limitations. PMID: 22249417 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalyt... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618318 Tue, 17 Jan 2012 05:00:00 +0100 5618318 http://www.medworm.com/index.php?rid=5618317&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22249418%26dopt%3DAbstract This study evaluates a new method of collecting urine samples on filter paper, dried urine spots (DUS), for simultaneous detection of EtG, EtS and creatinine, having the great advantage of inhibiting bacterial activity. In addition, a method validation for the determination of EtG and EtS in DUS was performed according to the FDA guidelines. Sterile-filtered urine was spiked with EtG and EtS, inoculated with E. coli and incubated. Liquid and dried urine samples were collected after various time intervals up to 96 h. Liquid samples were frozen immediately after collection, whereas aliquots for DUS were pipetted onto filter paper, allowed to dry and stored at RT until analysis 1 week after. The specimens were analyzed by LC-ESI-MS/MS. As expected, degradation of EtG, but not of EtS, was ob... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618317 Tue, 17 Jan 2012 05:00:00 +0100 5618317 http://www.medworm.com/index.php?rid=5618313&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22252656%26dopt%3DAbstract In this study, we determined an optimized BGE of 30 mM lactic acid/histidine plus 3 mM 18-crown-6 for the CIE-C4D system using the PVA capillary, which could simultaneously improve the separation of Mg(2+) and Ca(2+) from Na(+) and that of K(+) from NH (4) (+) . This system obtained highly reproducible separation of cations in human saliva samples within 8 min at 20 kV without deprotonation. The quantifiability of cations in human saliva samples on the CIE-C4D system was demonstrated through identification by ion chromatography with satisfactory results. PMID: 22252656 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618313 Mon, 16 Jan 2012 05:00:00 +0100 5618313 http://www.medworm.com/index.php?rid=5618319&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22246047%26dopt%3DAbstract Authors: Siebold M, Leidich P, Bertini M, Deflorio G, Feldmann J, Krupp EM, Halmschlager E, Woodward S Abstract Element distribution in the bark of two 20-year-old clones of Picea sitchensis following wounding was studied using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Bark was sampled at 0, 3, and 43 days after wounding and analysed using a focused Nd:YAG laser (266 nm). Intensities of (13) C, (25)Mg, (27)Al, (31)P, (32)S, (39)K, (48)Ca, (55)Mn, (57)Fe, (63)Cu and (64)Zn were measured by ICP-MS to study elemental distribution across the bark samples during the wound repair process. A clear accumulation of Mg, P and K at the boundary zone between the lesion and healthy tissue was detected in the wounded samples and was more distinctive at 43 than ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618319 Sun, 15 Jan 2012 05:00:00 +0100 5618319 http://www.medworm.com/index.php?rid=5618315&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22252654%26dopt%3DAbstract Authors: Gholap D, Verhulst J, Ceelen W, Vanhaecke F Abstract Quadrupole-based inductively coupled-mass spectrometry (ICP-MS) with pneumatic nebulization as a means of sample introduction was employed for quantification of platinum in blood and tissue samples of rats with peritoneal carcinomatosis, receiving intraperitoneal treatment with the Pt-containing chemotherapeutic drug oxaliplatin, and in the perfusate solution used for this purpose. The Pt levels were measured for various treatment conditions, i.e., with and without supporting treatment with the drug bevacizumab and at two different temperatures. Limits of detection obtained for platinum in blood and tissue samples were 0.3 and 2.0 pg g (,) (-1) respectively. Evaluation of drug pe... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618315 Sun, 15 Jan 2012 05:00:00 +0100 5618315 http://www.medworm.com/index.php?rid=5618314&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22252655%26dopt%3DAbstract Authors: Burhenne J, Halama B, Maurer M, Riedel KD, Hohmann N, Mikus G, Haefeli WE Abstract The benzodiazepine midazolam is a probe drug used to phenotype cytochrome P450 3A activity. In this situation, effective sedative concentrations are neither needed nor desired, and in fact the use of very low doses is advantageous. We therefore developed and validated an assay for the femtomolar quantification of midazolam and 1'-hydroxymidazolam in human plasma. Plasma (0.25 mL) and 96-well-based solid-phase extraction were used for sample preparation. Extraction recoveries ranged between 75 and 92% for both analytes. Extracts were chromatographed within 2 min on a Waters BEH C18 1.7 μm UPLC® column with a fast gradient consisting of formic acid, ammonia, and acetonitrile. Midazolam an... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618314 Sun, 15 Jan 2012 05:00:00 +0100 5618314 http://www.medworm.com/index.php?rid=5618320&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22245980%26dopt%3DAbstract This article reports on some of the interesting examples available in the literature regarding the use of metal-containing polymers, microgels and nanogels and thermoresponsive polymers. PMID: 22245980 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618320 Sat, 14 Jan 2012 05:00:00 +0100 5618320 http://www.medworm.com/index.php?rid=5618323&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22241580%26dopt%3DAbstract Authors: González-Antuña A, Rodríguez-González P, Lavandera I, Centineo G, Gotor V, García Alonso JI Abstract A novel and fast routine method for the simultaneous determination and confirmation of clenbuterol in bovine and human urine samples by gas chromatography electron ionization mass spectrometry (GC-EI-MS) has been developed. The method employs isotope dilution mass spectrometry (IDMS) and is based on a combination of minimal labeling (a single (13)C label in the molecule) and isotope pattern deconvolution (IPD). This new methodology does not require the construction of a methodological calibration graph, and was compared with the classical IDMS procedure employed in clenbuterol analysis based on the use of a deuterated compound as internal standard (d(9)-clenbuterol) an... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618323 Fri, 13 Jan 2012 05:00:00 +0100 5618323 http://www.medworm.com/index.php?rid=5618322&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22241581%26dopt%3DAbstract Authors: Gao X, Zhang H, Li Y, Su X Abstract In this paper, we report the successful use of non-cadmium-based Mn-doped ZnSe d-dots (Mn/ZnSe) as highly efficient and nontoxic optical probes for human prostate cancer cells imaging. Mn/ZnSe d-dots are directly prepared in aqueous solution. The α-methylacyl-CoA racemase (AMACR) is overexpressed in prostate cancers; the presence of antibodies specific for AMACR is more sensitive and specific than serum prostate specific antigen levels in distinguishing patients with prostate cancers. Mn/ZnSe d-dots were linked to anti-AMACR to form Mn/ZnSe d-dots-anti-AMACR bioconjugates for the direct prostate cancer cell imaging. 3-(4,5-Dimethylthiazol-2-yl)-2 and 5-diphenyl tetrazolium bromide assay demonstrated that Mn/ZnSe d-dots exhibited favorab... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618322 Fri, 13 Jan 2012 05:00:00 +0100 5618322 http://www.medworm.com/index.php?rid=5618321&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22245979%26dopt%3DAbstract Authors: Nguyen TT, Ostergaard J, Stürup S, Gammelgaard B Abstract A capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) method was developed for separation of the free oxaliplatin drug substance from liposome-entrapped oxaliplatin. Simultaneous determination of phosphorous and platinum opened the possibility to simultaneously monitor the liposomes (phospholipids) and platinum-based drug. In order to suppress the interferences, argon gas was used as a collision gas in ICP-MS. A detection limit of 29 ng/mL of platinum and a precision of 2.9% (for 10 μg/mL of oxaliplatin standard) were obtained. Measurement of the total concentration of free and encapsulated oxaliplatin by CE-ICP-MS was compared with total determination by ICP-MS after microwave dig... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618321 Fri, 13 Jan 2012 05:00:00 +0100 5618321 http://www.medworm.com/index.php?rid=5618324&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22237914%26dopt%3DAbstract In this study, the EC/DESI-MS method has been further applied to investigate some aqueous phase redox reactions of biological significance, including the reduction of peptide disulfide bonds and nitroaromatics as well as the oxidation of phenothiazines. It was found that knotted/enclosed disulfide bonds in the peptides apamin and endothelin could be electrochemically cleaved. Subsequent tandem MS analysis of the resulting reduced peptide ions using collision-induced dissociation (CID) and electron-capture dissociation (ECD) gave rise to extensive fragment ions, providing a fast protocol for sequencing peptides with complicated disulfide bond linkages. Flunitrazepam and clonazepam, a class of nitroaromatic drugs, are known to undergo reduction into amines which was proposed to involve nitro... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618324 Thu, 12 Jan 2012 05:00:00 +0100 5618324 http://www.medworm.com/index.php?rid=5618330&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22231509%26dopt%3DAbstract Authors: Borges EM, Euerby MR, Collins CH Abstract A novel stationary phase prepared by the thermal immobilization of poly(dimethylsiloxane) onto the surface of silica (PDMS-SiO(2)) has been described, evaluated and compared with 229 commercially available RP-LC stationary phases using the Tanaka column classification protocol. The phase exhibited many unique chromatographic properties and, based on the phases in the database, was most similar to the fluoroalkylated phases (aside from the obvious lack of fluoro selectivity imposed by the C-F dipole). The phase exhibited classic reversed-phase behaviour in acid mobile phase conditions and mixed-mode reversed-phase/cation-exchange retention behaviour in neutral mobile phase conditions. The phase exhibited acceptable stability at both... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618330 Wed, 11 Jan 2012 05:00:00 +0100 5618330 http://www.medworm.com/index.php?rid=5618326&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22234402%26dopt%3DAbstract Authors: Sander S, Arora N, Smith EA Abstract Cytoplasmic proteins that affect integrin diffusion in the cell membrane are identified using a combination of fluorescence recovery after photobleaching (FRAP) and RNA interference. Integrin receptors are essential for many cellular events, and alterations in lateral diffusion are one mechanism for modulating their function. In cells expressing native cytoplasmic protein concentrations and spread on a slide containing integrin extracellular ligand, 45 ± 2% of the integrin is mobile with a time-dependent 5.2 ± 0.9 × 10(-9) cm(2)/s diffusion coefficient at 1 s. The time exponent is 0.90 ± 0.07, indicating integrin diffusion moderately slows at longer times. The role of a specific cytoplasmic protein in altering inte... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618326 Wed, 11 Jan 2012 05:00:00 +0100 5618326 http://www.medworm.com/index.php?rid=5618325&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22234403%26dopt%3DAbstract Authors: Speltini A, Merli D, Dondi D, Paganini G, Profumo A Abstract Amino-terminated alkyl MWCNTs (MWCNTs-R-NH(2)), synthesized by chemical modification of the nanotube skeleton by nucleophilic substitution with 2,2'-(ethylenedioxy)diethylamine, were successfully used as stationary phases for gas chromatographic separation of esters and chloroaromatics. The presence of alkyl chains with polar embedded groups made the functionalized MWCNTs (f-MWCNTs) a mixed-mode GC separation material able to interact in different ways with the analytes. Compared with non-functionalized MWCNTs (nf-MWCNTs), MWCNTs-R-NH(2) had higher selectivity, enhanced resolution, and optimum retention behaviour, and they were proved to perform better than the commercial stationary phase Porapak QS (PQS), claime... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618325 Wed, 11 Jan 2012 05:00:00 +0100 5618325 http://www.medworm.com/index.php?rid=5618334&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22231505%26dopt%3DAbstract Authors: Bergner S PMID: 22231505 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618334 Tue, 10 Jan 2012 05:00:00 +0100 5618334 http://www.medworm.com/index.php?rid=5618333&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22231506%26dopt%3DAbstract Authors: Delhomme O, Raeppel C, Teigné D, Briand O, Millet M PMID: 22231506 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618333 Tue, 10 Jan 2012 05:00:00 +0100 5618333 http://www.medworm.com/index.php?rid=5618332&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22231507%26dopt%3DAbstract Authors: Thomas A, Geyer H, Schänzer W, Crone C, Kellmann M, Moehring T, Thevis M Abstract In the present study, a new type of mass spectrometer combining a quadrupole mass filter, a higher collision dissociation (HCD) cell and an Orbitrap detector, was evaluated for the analysis of dried blood spots (DBS) in doping controls. DBS analysis is characterized by the necessity to detect prohibited compounds in sub-nanogram-per-milliliter levels with high identification capacity. After extraction of DBS with an organic solvent and liquid chromatographic separation (using a regular C18-RP-analytical UHPLC-column) of target analytes, mass spectrometry is performed with a high-resolution full scan in positive and negative mode by means of electrospray ionisation. Single-product ion mass sp... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618332 Tue, 10 Jan 2012 05:00:00 +0100 5618332 http://www.medworm.com/index.php?rid=5618331&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22231508%26dopt%3DAbstract This study developed an ultraperformance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) method to quantitatively profile the major AN urinary metabolites (CEMA, HEMA, and CHEMA) to assess AN exposure, as well as analyze urinary cotinine (COT) as an indicator for tobacco smoke exposure. The limits of quantitation were 0.1, 0.1, 1.0, and 0.05 μg/L for HEMA, CEMA, CHEMA, and COT, respectively. This method was applied to analyze the three AN-derived MAs in 36 volunteers with no prior occupational AN exposure. Data analysis showed significant correlations between the level of COT and the levels of these MAs, suggesting them as biomarkers for exposure to low levels of AN. The results demonstrate that a highly specific and sensitive UPLC-MS/MS method has been successfu... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618331 Tue, 10 Jan 2012 05:00:00 +0100 5618331 http://www.medworm.com/index.php?rid=5618329&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22231510%26dopt%3DAbstract Authors: Mueller DM, Rentsch KM Abstract Human liver microsomes (HLMs) are used to simulate human xenobiotic metabolism in vitro. In forensic and clinical toxicology, HLMs are popularly used to study the metabolism of new designer drugs for example. In this work, we present an automated online extraction system we developed for HLM experiments, which was compared to a classical offline approach. Furthermore, we present studies on the metabolism of 11 cathinones; for eight of these, the metabolism has not previously been reported. Metabolites were identified based on MS(2) and MS(3) scans. Fifty-three substances encompassing various classes of drugs were employed to compare the established offline and the new online methods. The metabolism of each of the following 11 cathinones was ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618329 Tue, 10 Jan 2012 05:00:00 +0100 5618329 http://www.medworm.com/index.php?rid=5618328&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22231511%26dopt%3DAbstract Authors: Duce C, Ghezzi L, Onor M, Bonaduce I, Colombini MP, Tine' MR, Bramanti E Abstract In this work, we characterized paint reconstructions using ovalbumin and casein as binders, and cinnabar (HgS) as a pigment, before and after artificial ageing. Egg and casein are common paint binders that were used historically in the technique of tempera painting. Despite extensive research on the identification of proteinaceous binders in paintings, there is a substantial lack of knowledge regarding the ageing pathway of their protein content, and their chemical interaction with inorganic pigments. Thermogravimetric analysis, infrared spectroscopy and size-exclusion chromatography (SEC) were used to reveal the physico-chemical processes involved in the ageing of proteins in paintings. Take... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618328 Tue, 10 Jan 2012 05:00:00 +0100 5618328 http://www.medworm.com/index.php?rid=5618327&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22231512%26dopt%3DAbstract In this study, we investigated the metabolism and fragmentation pattern of β-END in rat inflamed tissue, in rat serum and in trypsin solution. β-END (1-31)-rat was incubated at 37 °C in each matrix for different incubation times. The resultant fragments were separated using a C4 column and detected by mass spectrometry using total ion current mode. Structural information for the fragments was elucidated using tandem mass spectrometry. Incubation of β-END (1-31)-rat in trypsin solution and in rat serum resulted in 8 and 13 fragments, respectively. Incubation in inflamed rat paw tissue resulted in 22 fragments at pH 7.4 and 26 fragments at pH 5.5. Some of these fragments were common to both pH values. The degradation of β-END (1-31)-rat in inflamed tissue at pH 5.5 was faster than t... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618327 Tue, 10 Jan 2012 05:00:00 +0100 5618327 http://www.medworm.com/index.php?rid=5618336&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227826%26dopt%3DAbstract Authors: Faber H, Melles D, Brauckmann C, Wehe CA, Wentker K, Karst U Abstract Diclofenac is a frequently prescribed drug for rheumatic diseases and muscle pain. In rare cases, it may be associated with a severe hepatotoxicity. In literature, it is discussed whether this toxicity is related to the oxidative phase I metabolism, resulting in electrophilic quinone imines, which can subsequently react with nucleophiles present in the liver in form of glutathione or proteins. In this work, electrochemistry coupled to mass spectrometry is used as a tool for the simulation of the oxidative pathway of diclofenac. Using this purely instrumental approach, diclofenac was oxidized in a thin layer cell equipped with a boron doped diamond working electrode. Sum formulae of generated oxidation pr... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618336 Mon, 09 Jan 2012 05:00:00 +0100 5618336 http://www.medworm.com/index.php?rid=5618335&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227827%26dopt%3DAbstract Authors: Deflorio G, Horgan G, Jaspars M, Woodward S Abstract Metabolite fingerprinting of Sitka spruce (Picea sitchensis) bark and sapwood was carried out by (1)H nuclear magnetic resonance after wounding and artificial inoculation with the white rot fungus Heterobasidion annosum sensu stricto. The aim was to determine whether metabolites would differ in clones showing differing levels of susceptibility to H. annosum, in the fungal as compared with the control treatment (wounding, no fungus) and the reference (healthy sample collected at 0 days), at two different locations on the host, and at different sampling times (3 and 43 days after treatment). The results suggested that different metabolic processes occur in bark and sapwood after wounding and fungal inoculation, compared ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618335 Mon, 09 Jan 2012 05:00:00 +0100 5618335 http://www.medworm.com/index.php?rid=5618344&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227744%26dopt%3DAbstract Authors: Kundel M, Thorenz UR, Petersen JH, Huang RJ, Bings NH, Hoffmann T Abstract Knowledge of the composition and emission rates of iodine-containing volatiles from major widespread seaweed species is important for modeling the impact of halogens on gas-phase atmospheric chemistry, new particle formation, and climate. In this work, we present the application of mass spectrometric techniques for the quantification of short-lived iodine-containing volatiles emitted by eight different seaweeds from the intertidal zone of Helgoland, Germany. A previously developed online time-of-flight aerosol mass spectrometric method was used to determine I(2) emission rates and investigate temporally resolved emission profiles. Simultaneously, iodocarbons were preconcentrated on solid adsorbent t... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618344 Sun, 08 Jan 2012 05:00:00 +0100 5618344 http://www.medworm.com/index.php?rid=5618342&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227746%26dopt%3DAbstract Authors: Melles D, Vielhaber T, Baumann A, Zazzeroni R, Karst U Abstract Historically, skin sensitization tests are typically based on in vivo animal tests. However, for substances used in cosmetic products, these tests have to be replaced according to the European Commission regulation no. 1223/2009. Modification of skin proteins by electrophilic chemicals is a key process associated with the induction of skin sensitization. The present study investigates the capabilities of a purely instrumental setup to determine the potential of commonly used non-electrophilic chemicals to cause skin sensitization by the generation of electrophilic species from the parent compound. In this work, the electrophiles were generated by the electrochemical oxidation of aniline, a basic industrial che... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618342 Sun, 08 Jan 2012 05:00:00 +0100 5618342 http://www.medworm.com/index.php?rid=5618341&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227747%26dopt%3DAbstract Authors: Sciutto G, Oliveri P, Prati S, Quaranta M, Lanteri S, Mazzeo R Abstract The present research is aimed at introducing a suitable approach for the exploitation of the hyperspectral data obtained by μATR-FTIR analyses of paint cross-sections. The application of principal component analysis for chemical mapping is well-established, even if a very limited number of applications to μFTIR data have been reported so far in the field of analytical chemistry for cultural heritage. Moreover, in many cases, chemometric tools are under-utilized and the outcomes under-interpreted. As a consequence, results and conclusions may be considerably compromised. In an attempt to overcome such drawbacks, the present work is proposing a comprehensive and efficient procedure based on an interact... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618341 Sun, 08 Jan 2012 05:00:00 +0100 5618341 http://www.medworm.com/index.php?rid=5618340&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227748%26dopt%3DAbstract Authors: Chiarandini JP, Escandar GM Abstract The fluorimetric signal produced by bentazone retained in selected solid surfaces was investigated. Among the different tested supports, only a microporous nylon membrane produced the desired signal. The quantitative study was carried out by second-order calibration using parallel factor analysis, allowing the determination in a highly interfering medium. A detection limit of 0.4 ng mL(-1), a prediction relative error of 8%, and a sample frequency of ten samples per hour were obtained in spiked natural waters using green analytical chemistry principles. PMID: 22227748 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618340 Sun, 08 Jan 2012 05:00:00 +0100 5618340 http://www.medworm.com/index.php?rid=5618339&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227811%26dopt%3DAbstract Authors: Watarai H PMID: 22227811 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618339 Sun, 08 Jan 2012 05:00:00 +0100 5618339 http://www.medworm.com/index.php?rid=5618338&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227812%26dopt%3DAbstract In this study, an integrated approach is developed for the formation, identification and biological characterization of electrochemical conversion products of p38α mitogen-activated protein kinase inhibitors. This work demonstrates the hyphenation of an electrochemical reaction cell with a continuous-flow bioaffinity assay and parallel LC-HR-MS. Competition of the formed products with a tracer (SKF-86002) that shows fluorescence enhancement in the orthosteric binding site of the p38α kinase is the readout for bioaffinity. Parallel HR-MS(n) experiments provided information on the identity of binders and non-binders. Finally, the data produced with this on-line system were compared to electrochemical conversion products generated off-line. The electrochemical conversion of 1-{6-chloro-5-[(... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618338 Sun, 08 Jan 2012 05:00:00 +0100 5618338 http://www.medworm.com/index.php?rid=5618337&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227813%26dopt%3DAbstract Authors: Chen Z, Zhang G, Chen X, Gao W Abstract On the basis of the resonance light scattering (RLS) of Ag nanoparticles (AgNPs), an RLS off-on system was developed for studies of the selective interaction between adriamycin (ADM) and DNA. In this strategy, addition of ADM could induce a proportional decrease in the RLS intensity of AgNPs; this could be used to detect trace amounts of ADM with a detection limit of 12.75 ng mL(-1) in the range 0.021-10.0 μg mL(-1). Subsequently, by investigating the ability of different DNA sequences to restore the RLS intensity of the analytical systems, we found that ADM was selective to dsDNA and had an obvious preference for sequences that were rich in guanine and cytosine bases. In order to validate the results of the RLS assay, fluoresce... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618337 Sun, 08 Jan 2012 05:00:00 +0100 5618337 http://www.medworm.com/index.php?rid=5618343&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22227745%26dopt%3DAbstract Authors: Perez F, Llorca M, Farré M, Barceló D Abstract Perfluorinated compounds (PFCs) are ubiquitous contaminants of humans and animals worldwide. PFCs are bioaccumulated because of their affinity for proteins. It has been shown they could have a variety of toxicological effects and cause damage to human health, emphasizing the need for sensitive and robust analytical methods to assess their bioaccumulation in humans. In this paper we report the development and validation of an analytical method for analysis of PFCs in the non-invasive human matrices hair and urine. The method is based on rapid and simple sample pre-treatment followed by online turbulent flow liquid chromatography and tandem mass spectrometry (TFC-LC-MS-MS) for analysis of 21 PFCs. The method was validated for ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5618343 Sat, 07 Jan 2012 05:00:00 +0100 5618343 http://www.medworm.com/index.php?rid=5575735&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22222912%26dopt%3DAbstract Authors: Song KM, Jeong E, Jeon W, Cho M, Ban C Abstract A gold nanoparticle based dual fluorescence-colorimetric method was developed as an aptasensor to detect ampicillin using its single-stranded DNA (ssDNA) aptamer, which was discovered by a magnetic bead-based SELEX technique. The selected aptamers, AMP4 (5'-CACGGCATGGTGGGCGTCGTG-3'), AMP17 (5'-GCGGGCGGTTGTATAGCGG-3'), and AMP18 (5'-TTAGTTGGGGTTCAGTTGG-3'), were confirmed to have high sensitivity and specificity to ampicillin (K (d), AMP7 = 9.4 nM, AMP17 = 13.4 nM, and AMP18 = 9.8 nM, respectively). The 5'-fluorescein amidite (FAM)-modified aptamer was used as a dual probe for observing fluorescence differences and color changes simultaneously. The lower limits of detection for this dual method were a ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575735 Sat, 07 Jan 2012 05:00:00 +0100 5575735 http://www.medworm.com/index.php?rid=5575733&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22222914%26dopt%3DAbstract Authors: Fu SC, Tzing SH, Chen HC, Wang YC, Ding WH Abstract A simple sample pretreatment technique, dispersive micro-solid phase extraction, was applied for the extraction of N-nitrosodimethylamine (NDMA) and other four N-nitrosamines (NAs) from samples of swimming pool water. The parameters affecting the extraction efficiency were systematically investigated. The best extraction conditions involved immersing 75 mg of carbon molecular sieve, Carboxen™ 1003 (as an adsorbent), in a 50-mL water sample (pH 7.0) containing 5% sodium chloride in a sample tube. After 20 min of extraction by vigorous shaking, the adsorbent was collected on a filter and the NAs desorbed by treatment with 150 μL of dichloromethane. A 10-μL aliquot was then directly determined by large-volume injecti... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575733 Sat, 07 Jan 2012 05:00:00 +0100 5575733 http://www.medworm.com/index.php?rid=5575736&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22222911%26dopt%3DAbstract In this study, different flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenases (FADGDHs) were characterized electrochemically after "wiring" them with an osmium redox polymer [Os(4,4'-dimethyl-2,2'-bipyridine)(2)(PVI)(10)Cl](+) on graphite electrodes. One tested FADGDH was that recently discovered in Glomerella cingulata (GcGDH), another was the recombinant form expressed in Pichia pastoris (rGcGDH), and the third was a commercially available glycosylated enzyme from Aspergillus sp. (AspGDH). The performance of the Os-polymer "wired" GDHs on graphite electrodes was tested with glucose as the substrate. Optimal operational conditions and analytical characteristics like sensitivity, linear ranges and current density of the different FADGDHs were determined. The performance of al... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575736 Fri, 06 Jan 2012 05:00:00 +0100 5575736 http://www.medworm.com/index.php?rid=5575734&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22222913%26dopt%3DAbstract This study provides a versatile validated method to determine the total vitamin C content, as the sum of the contents of L-ascorbic acid (L-AA) and dehydroascorbic acid (DHAA), in several fruits and vegetables and its degradability with storage time. Seven horticultural crops from two different origins were analyzed using an ultra-high-performance liquid chromatographic-photodiode array (UHPLC-PDA) system, equipped with a new trifunctional high strength silica (100% silica particle) analytical column (100 mm × 2.1 mm, 1.7 μm particle size) using 0.1% (v/v) formic acid as mobile phase, in isocratic mode. This new stationary phase, specially designed for polar compounds, overcomes the problems normally encountered in HPLC and is suitable for the analysis of large batches of samples ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575734 Fri, 06 Jan 2012 05:00:00 +0100 5575734 http://www.medworm.com/index.php?rid=5575738&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22218462%26dopt%3DAbstract Authors: Chen CZ, Sobczak K, Hoskins J, Southall N, Marugan JJ, Zheng W, Thornton CA, Austin CP Abstract Myotonic dystrophy type 1 (DM1), the most prevalent form of adult muscular dystrophy, is caused by expansion of a CTG repeat in the 3' untranslated region of the DM protein kinase (DMPK) gene. The pathogenic effects of the CTG expansion arise from the deleterious effects of the mutant transcript. RNA with expanded CUG tracts alters the activities of several RNA binding proteins, including muscleblind-like 1 (MBNL1). MBNL1 becomes sequestered in nuclear foci in complex with the expanded CUG-repeat RNA. The resulting loss of MBNL1 activity causes misregulated alternative splicing of multiple genes, leading to symptoms of DM1. The binding interaction between MBNL1 and mutant RNA co... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575738 Thu, 05 Jan 2012 05:00:00 +0100 5575738 http://www.medworm.com/index.php?rid=5575737&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22218463%26dopt%3DAbstract Authors: Dong L, Zang C, Wang J, Li L, Gao Y, Wu L, Li P Abstract Quantification of genomic DNA that is traceable to the SI was performed successfully by measuring the individual nucleotides. Specifically, ultrasound was used to shear lambda genomic DNA into fragments of less than 200 base pairs, followed by deoxyribonuclease Ι and phosphodiesterase Ι digestion and liquid chromatography-isotope dilution mass spectrometry (LC-IDMS) quantification to estimate the mass fraction of the lambda DNA, based on the constituent deoxynucleotide monophosphates (dNMPs) within the molecule. Digital PCR (dPCR) was employed to quantify the same lambda DNA solution to provide independent data for comparing the performance of two quantitative methods. On the basis of the LC-IDMS measurement after ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575737 Thu, 05 Jan 2012 05:00:00 +0100 5575737 http://www.medworm.com/index.php?rid=5575739&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22215246%26dopt%3DAbstract Authors: Du F, Ruan G, Liu H Abstract Plant hormones play important roles in regulating numerous aspects of plant growth, development, and response to stress. In the past decade, more analytical methods for the accurate identification and quantitative determination of trace plant hormones have been developed to better our understanding of the molecular mechanisms of plant hormones. As sample preparation is often the bottleneck in analysis of plant hormones in biological samples, this review firstly discusses sample preparation techniques after a brief introduction to the classes, roles, and methods used in the analysis of plant hormones. The analytical methods, especially chromatographic techniques and immuno-based methods, are reviewed in detail, and their corresponding advantages... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575739 Wed, 04 Jan 2012 05:00:00 +0100 5575739 http://www.medworm.com/index.php?rid=5575742&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22212864%26dopt%3DAbstract Authors: Zanoli LM, Licciardello M, D'Agata R, Lantano C, Calabretta A, Corradini R, Marchelli R, Spoto G Abstract The use of droplet-based microfluidics and peptide nucleic acid molecular beacons for the detection of polymerase chain reaction (PCR)-amplified DNA sequences within nanoliter-sized droplets is described in this work. The nanomolar-attomolar detection capabilities of the method were preliminarily tested by targeting two different single-stranded DNA sequences from the genetically modified Roundup Ready soybean and the Olea europaea genomes and detecting the fluorescence generated by peptide nucleic acid molecular beacons with fluorescence microscopy. Furthermore, the detection of 10 nM solutions of PCR amplicon of DNA extracted from leaves of O. europaea L. encapsulate... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575742 Tue, 03 Jan 2012 05:00:00 +0100 5575742 http://www.medworm.com/index.php?rid=5575741&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22212887%26dopt%3DAbstract Authors: Meija J PMID: 22212887 [PubMed - in process] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575741 Sun, 01 Jan 2012 05:00:00 +0100 5575741 http://www.medworm.com/index.php?rid=5575740&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22212888%26dopt%3DAbstract Authors: Hashemi M, Chasteen TG PMID: 22212888 [PubMed - in process] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5575740 Sun, 01 Jan 2012 05:00:00 +0100 5575740 http://www.medworm.com/index.php?rid=5557719&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209956%26dopt%3DAbstract Authors: Lim CW, Tai SH, Lee LM, Chan SH Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and MS(3) quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were s... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557719 Sat, 31 Dec 2011 05:00:00 +0100 5557719 http://www.medworm.com/index.php?rid=5557718&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22209957%26dopt%3DAbstract Authors: Yan Z, Chen C, Xie X, Fu B, Yang X Abstract A rapid ultra-high-performance liquid chromatographic-tandem mass spectrometric (UHPLC-MS-MS) method has been developed for rapid screening and quantitative analysis of sulfonate derivatives (SDs) in commercial white peony root. Separation was performed on an Agilent Zorbax Eclipse Plus-C18 column by gradient elution with acetonitrile-0.1% (v/v) formic acid as the mobile phase. In-source fragmentation was used to generate the characteristic fragment ion at m/z 259 and to screen for nine SDs. Detection of these SDs was further performed in multiple reaction monitoring (MRM) mode to improve sensitivity and to quantify the two SDs paeoniflorin sulfonate and benzoylpaeoniflorin sulfonate. The method was validated for specificity, lin... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557718 Sat, 31 Dec 2011 05:00:00 +0100 5557718 http://www.medworm.com/index.php?rid=5557721&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207281%26dopt%3DAbstract Authors: Shelver WL, Varel VH Abstract An ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed capable of simultaneously measuring chlortetracycline (CTC), epi-chlortetracycline, and isochlortetracycline (ICTC), as well as other structurally related tetracyclines in swine manure. A simple sample preparation was used consisting of extraction, dilution, centrifugation, and ultrafiltration. The concentrations of analyte were calculated using d(6)-tetracycline as an internal standard in the matrix-matched standard curve. A solvent gradient resolved the compounds in 3.5 min with an additional 1.5 min of re-equilibration allowing the analyses of a large number of samples in a short period of time. MS/MS was used as the detection metho... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557721 Fri, 30 Dec 2011 05:00:00 +0100 5557721 http://www.medworm.com/index.php?rid=5557720&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22207282%26dopt%3DAbstract Authors: Amézqueta S, Galán E, Fuguet E, Carrascal M, Abián J, Torres JL Abstract D: -Fagomine is an iminosugar first found in buckwheat (Fagopyrum esculentum Moench) which if used as a dietary supplement or functional food component may reduce the risks of developing insulin resistance, becoming overweight and suffering from an excess of potentially pathogenic bacteria. As D: -fagomine may become increasingly important to the food industry, a reliable analytical method for its determination in natural plant sources and foodstuffs is desirable. We have devised a method to separate D: -fagomine from its diastereomers 3-epi-fagomine and 3,4-di-epi-fagomine in a single run by cation exchange high-performance liquid chromatography (HPLC) with detection and quantification by mass spe... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557720 Fri, 30 Dec 2011 05:00:00 +0100 5557720 http://www.medworm.com/index.php?rid=5557727&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203370%26dopt%3DAbstract Authors: Shah SS, Kim M, Foster E, Vu T, Patel D, Chen LJ, Verkhoturov SV, Schweikert E, Tae G, Revzin A Abstract This paper describes a novel platform that utilizes micropatterning and electrochemistry to release cells-on-hydrogel microstructures from conductive indium tin oxide (ITO) substrates. In this approach, UV photopolymerization was employed to micropattern heparin-based hydrogels onto glass substrates containing ITO electrodes. ITO/glass substrates were first functionalized with acrylated silane to promote attachment of hydrogel structures. The surfaces containing hydrogel micropatterns were further functionalized with poly(ethylene glycol) thiol, rendering the regions around the hydrogel structures non-fouling to proteins and cells. After incubating surfaces with collage... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557727 Thu, 29 Dec 2011 05:00:00 +0100 5557727 http://www.medworm.com/index.php?rid=5557725&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203372%26dopt%3DAbstract Authors: Favaro M, Guastoni A, Marini F, Bianchin S, Gambirasi A Abstract In this paper, we propose an analytical methodology for attributing provenance to natural lapis lazuli pigments employed in works of art, and for distinguishing whether they are of natural or synthetic origin. A multitechnique characterization of lazurite and accessory phases in lapis lazuli stones from Afghan, Siberian and Chilean quarries, on the pigments obtained by their purification, and on synthetic ultramarine pigments was performed. According to the results obtained, infrared spectroscopy is not a suitable technique for distinguishing the provenance of lapis lazuli, but a particular absorbance band makes it relatively easy to determine whether it is of natural or synthetic origin. On the other hand, E... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557725 Thu, 29 Dec 2011 05:00:00 +0100 5557725 http://www.medworm.com/index.php?rid=5557724&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203373%26dopt%3DAbstract Authors: Cruz MP, Valente IM, Gonçalves LM, Rodrigues JA, Barros AA Abstract In wines, the presence of high levels of acetaldehyde (AA) not only is responsible for undesirable characteristic odours but can also cause health adverse effects. Such sensorial activity of AA can be overcome by adding sulphites during winemaking, due to the formation of adducts between AA and sulphites, which lower the sensorial impact of AA. Nevertheless, bound AA can be released during wine storage; therefore, the knowledge of its total amount can be important to estimate the long-term wine quality. The proposed methodology is based on the extraction of AA from wines using gas-diffusion microextraction and determination by liquid chromatography. Free and bound forms of AA could be differentiated and d... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557724 Thu, 29 Dec 2011 05:00:00 +0100 5557724 http://www.medworm.com/index.php?rid=5557723&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22205182%26dopt%3DAbstract Authors: Cialla D, März A, Böhme R, Theil F, Weber K, Schmitt M, Popp J Abstract Surface-enhanced Raman spectroscopy (SERS) combines molecular fingerprint specificity with potential single-molecule sensitivity. Therefore, the SERS technique is an attractive tool for sensing molecules in trace amounts within the field of chemical and biochemical analytics. Since SERS is an ongoing topic, which can be illustrated by the increased annual number of publications within the last few years, this review reflects the progress and trends in SERS research in approximately the last three years. The main reason why the SERS technique has not been established as a routine analytic technique, despite its high specificity and sensitivity, is due to the low reproducibility of the SERS signal. Thu... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557723 Thu, 29 Dec 2011 05:00:00 +0100 5557723 http://www.medworm.com/index.php?rid=5557722&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22205183%26dopt%3DAbstract Authors: Erger C, Balsaa P, Werres F, Schmidt TC Abstract Solid-phase extraction (SPE) is a widespread and powerful sample preparation technique in many analytical areas. Many of the used methods reduce residual water during sample preparation by drying the phase material. Despite the importance of this step, hardly any study deals specifically with the drying process, and if so, only few aspects are mentioned. The present study is the first systematic investigation of the drying process using SPE disks, including the influence of process parameters on the amount of residual water and its consequences for subsequent elution and gas chromatography-mass spectrometry (GC-MS) analysis. The following points were investigated in detail: (1) the change of pressure and volume flow during t... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557722 Thu, 29 Dec 2011 05:00:00 +0100 5557722 http://www.medworm.com/index.php?rid=5557726&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22203371%26dopt%3DAbstract Authors: Hopfgartner G, Tonoli D, Varesio E Abstract Quantitative and qualitative high-resolution (HR) dependent and independent acquisition schemes on a QqTOF MS (with resolving power 20,000-40,000) were investigated for the analysis of pharmaceutical compounds in biological fluids. High-resolution selected reaction monitoring (HR-SRM) was found to be linear over three orders of magnitude for quantitative analysis of paracetamol in human plasma, offering a real alternative to triple quadrupole LC-SRM/MS. Metabolic stability of talinolol in microsomes was characterized by use of three different acquisition schemes: (i) information-dependent acquisition (IDA) with a TOF MS experiment as survey scan and product-ion scan as dependent scan; (ii) MS(ALL) by collecting TOF mass spectra w... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557726 Wed, 28 Dec 2011 05:00:00 +0100 5557726 http://www.medworm.com/index.php?rid=5557737&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200920%26dopt%3DAbstract Authors: Zeng X, Andrade CA, Oliveira MD, Sun XL Abstract Carbohydrate recognition is clearly present throughout nature, playing a major role in the initial attachment of one biological entity to another. The important question is whether these prevalent interactions could provide a real suitable alternative to the use of antibodies or nucleic acid for detection and identification. Currently, examples of carbohydrates being employed in biological detection systems are limited. The challenges of using carbohydrate recognition for detection mainly come from the weak affinity of carbohydrate-protein interactions, the lack of versatile carbohydrate scaffolds with well-defined structures, and the less developed high-information-content, real-time, and label-free assay technology. In thi... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557737 Tue, 27 Dec 2011 05:00:00 +0100 5557737 http://www.medworm.com/index.php?rid=5557736&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200921%26dopt%3DAbstract This article focuses on the benefits of combining molecular biology and synchrotron-based techniques. By using molecular techniques to probe the location of gene expression and protein production in combination with laterally resolved synchrotron techniques, one can effectively and efficiently assign functional information to specific genes. A review of the state of the art in this field is presented, together with examples as to how synchrotron-based methods can be combined with molecular techniques to facilitate functional characterisation of genes in planta. The article concludes with a summary of the technical challenges still remaining for synchrotron-based hard X-ray plant science research, particularly those relating to subcellular level research. PMID: 22200921 [PubMed - as sup... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557736 Tue, 27 Dec 2011 05:00:00 +0100 5557736 http://www.medworm.com/index.php?rid=5557735&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200922%26dopt%3DAbstract Authors: Fabrizi G, Fioretti M, Mainero Rocca L, Curini R Abstract With the aim of establishing exposure levels for hospital personnel preparing and administering cytostatic drugs (CDs), here, we present an innovative screening method based on the use of the desorption electrospray ionization (DESI) interface coupled with a hybrid quadrupole linear ion trap mass spectrometer. A rapid, simple, and sensitive procedure was developed for the simultaneous surface monitoring of cyclophosphamide, dacarbazine, methotrexate, vincristine, gemcitabine, and cytarabine. Since analytes were in the solid state, a novel approach based on the use of passive samplers was combined with the direct analysis of wipes. A PTFE-printed glass slide was used as a passive sampler, while hydrophobic centers of... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557735 Tue, 27 Dec 2011 05:00:00 +0100 5557735 http://www.medworm.com/index.php?rid=5557734&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200923%26dopt%3DAbstract Authors: Pinto G, Caira S, Cuollo M, Fierro O, Nicolai MA, Chianese L, Addeo F Abstract Casein phosphopeptides (CPP) were identified in small amounts in milks heated at various intensities by using matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. CPP selectively concentrated on hydroxyapatite (HA) were regenerated using phosphoric acid mixed in the matrix. Unphosphorylated peptides not retained by HA were removed by buffer washing. This procedure enhanced the MALDI signals of CPP that are ordinarily suppressed by the co-occurrence of unphosphorylated peptides. CPP, belonging to the β-casein (CN) family, i.e., (f1-29) 4P, (f1-28) 4P, and (f1-27) 4P, and the α(s2)-CN family, i.e., (f1-21) 4P and (f1-24) 4P, were observed in liquid and powder mil... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557734 Tue, 27 Dec 2011 05:00:00 +0100 5557734 http://www.medworm.com/index.php?rid=5557733&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200924%26dopt%3DAbstract Authors: Sabela MI, Gumede NJ, Escuder-Gilabert L, Martín-Biosca Y, Bisetty K, Medina-Hernández MJ, Sagrado S Abstract The stereoselective binding of the frequently ingested nutraceutical (±)-catechin, with demonstrated differential biological activity between enantiomers, to human serum albumin (HSA), with the largest complexation and enantioselectivity potential among the plasmatic proteins, is studied by combining simulations to optimize the experimental design, robust in vitro electrokinetic chromatographic data, and molecular docking-chiral recognition estimates. Methodological and mathematical drawbacks in previous reports on (±)-catechin-HSA are detected and eliminated. Recent and novel direct equations extracted from the classical interaction model allows advantageous u... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557733 Tue, 27 Dec 2011 05:00:00 +0100 5557733 http://www.medworm.com/index.php?rid=5557732&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200925%26dopt%3DAbstract Authors: Kim JS, Song JS, Kim Y, Park SB, Kim HJ Abstract De novo analysis of protein N-terminal sequence is important for identification of N-terminal proteolytic processing such as N-terminal methionine or signal peptide removal, or for the genome annotation of uncharacterized proteins. We introduce a de novo sequencing method of protein N terminus utilizing matrix-assisted laser desorption/ionization (MALDI) signal enhancing picolinamidination with bromine isotopic tag incorporated to the N terminus. The doublet signature of bromine in the tandem mass (MS/MS) spectrum distinguished N-terminal ion series from C-terminal ion series, facilitating de novo N-terminal sequencing of protein. The dual advantage of MALDI signal enhancement by the basic picolinamidine and b-ion selection ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557732 Tue, 27 Dec 2011 05:00:00 +0100 5557732 http://www.medworm.com/index.php?rid=5557731&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200926%26dopt%3DAbstract Authors: Akahoshi N, Ishizaki I, Naya M, Maekawa T, Yamazoe S, Horiuchi T, Kajimura M, Ohashi Y, Suematsu M, Ishii I Abstract In vivo imaging of reactive small molecule metabolites with high spatial resolution and specificity could give clues to understanding pathophysiology of various diseases. We herein applied time of flight-secondary ion mass spectrometry (TOF-SIMS) to newly developed silver-deposited plates that were stamped on mouse tissues, and succeeded in visualization of halide (Cl(-), Br(-), and I(-)) and pseudohalide thiocyanate (SCN(-)) anions, a class of substrates for neutrophils/eosinophil peroxidases to produce hypohalous acids (HOX/OX(-) mixture; X: (pseudo)halides), as well as hydrogen sulfide (H(2)S). Forty-micrometer frozen mouse kidney sections on cover glasse... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557731 Tue, 27 Dec 2011 05:00:00 +0100 5557731 http://www.medworm.com/index.php?rid=5557730&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200927%26dopt%3DAbstract This article details the development of a novel method that overcomes the drawbacks of sandwich ELISA (sELISA) and allows reliable evaluation of simultaneous quantification of the amyloid (Aβ)-peptides, total-Aβ, Aβx-38, Aβx-40, and Aβx-42, in rat brain by optimized sample purification and column-switching liquid chromatographic-tandem mass spectrometry (LC/MS/MS). This method provides accurate analyses of total-Aβ, Aβx-38, Aβx-40, and Aβx-42 with a linear calibration range between 0.05 and 45 ng/mL. Verification for accuracy and precision of biological samples were determined by a standard addition and recovery test, spiked with synthetic Aβ1-38, Aβ1-40, and Aβ1-42 into the rat brain homogenate. This method showed <20% relative error and relative standard deviation, indica... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557730 Tue, 27 Dec 2011 05:00:00 +0100 5557730 http://www.medworm.com/index.php?rid=5557729&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200928%26dopt%3DAbstract Authors: Ma C, Yeung ES, Qi S, Han R Abstract We have developed a new methodology for miRNA assay using chemiluminescence imaging by poly(U) polymerase catalyzed miRNA polymerization. This method is very sensitive with a 50 fM limit of detection, which is comparable to or better than current assay methods. Multiplex detection for miRNA can be easily realized by introducing different capture probes onto the biosensor array, which will make it highly versatile for various research purposes. PMID: 22200928 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557729 Tue, 27 Dec 2011 05:00:00 +0100 5557729 http://www.medworm.com/index.php?rid=5557728&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22200929%26dopt%3DAbstract Authors: Sogawa K, Watanabe M, Sato K, Segawa S, Miyabe A, Murata S, Saito T, Nomura F Abstract Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used as a microbial diagnostic method for species identification of pathogens. However, MALDI-TOF identification of bacteria at the species level remains unsatisfactory, with the major problem being an incomplete database that still needs refinement and expansion. Augmentation of the original MALDI BioTyper 2.0 (Bruker) database by incorporating mass spectra obtained in-house from clinical isolates may increase the identification rate at the species level. We conducted a prospective study to assess whether the augmented database can improve the performance of MALDI-TOF MS for routi... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5557728 Tue, 27 Dec 2011 05:00:00 +0100 5557728 http://www.medworm.com/index.php?rid=5544095&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22193819%26dopt%3DAbstract Authors: Morini L, Colucci M, Ruberto MG, Groppi A Abstract A liquid chromatography tandem mass spectrometry method for ethyl glucuronide (EtG) detection and quantification in nails was developed and fully validated. Nails were extracted in 700 μL double-distilled water. EtG-d (5) was used as an internal standard. Reversed-phase separation was obtained with an isocratic mobile phase composed of 0.1% formic acid and acetonitrile (99:1) for 10 min. Quantification was performed by multiple reaction monitoring of two transitions per compound (EtG and internal standard). The assay was linear from 10 to 500 pg/mg. Validation parameters were studied at three different quality control levels (10, 50, and 300 pg/mg). Intraday, interday, and total imprecision had a coefficient ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5544095 Fri, 23 Dec 2011 05:00:00 +0100 5544095 http://www.medworm.com/index.php?rid=5544096&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22189629%26dopt%3DAbstract Authors: Cotrone S, Cafagna D, Cometa S, De Giglio E, Magliulo M, Torsi L, Sabbatini L Abstract Most of the success of electronic devices fabricated to actively interact with a biological environment relies on the proper choice of materials and efficient engineering of surfaces and interfaces. Organic materials have proved to be among the best candidates for this aim owing to many properties, such as the synthesis tunability, processing, softness and self-assembling ability, which allow them to form surfaces that are compatible with biological tissues. This review reports some research results obtained in the development of devices which exploit organic materials' properties in order to detect biologically significant molecules as well as to trigger/capture signals from the biologi... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5544096 Thu, 22 Dec 2011 05:00:00 +0100 5544096 http://www.medworm.com/index.php?rid=5544098&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22186871%26dopt%3DAbstract Authors: Schocker A, Lißner B Abstract Open-loop stripping analysis (also referred to as dynamic headspace) is a very flexible and robust technology for online monitoring of volatile organic compounds in wastewater or coolant. However, the quality and reliability of the analytical results depend strongly on the temperature during the stripping process. Hence, the careful and constant heating of the liquid phase inside the stripping column is a critical parameter. In addition, this stripping at high temperatures extends the spectrum of traceable organics to less volatile and more polar compounds with detection limits down to the ppm-level. This paper presents a novel and promising approach for fast, efficient, and constant heating by the direct injection of process steam into the s... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5544098 Wed, 21 Dec 2011 05:00:00 +0100 5544098 http://www.medworm.com/index.php?rid=5544097&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22186872%26dopt%3DAbstract Authors: Kondo F, Okada S, Miyachi A, Kurita M, Tsuji K, Harada KI Abstract The reaction of some physiologically active peptides with bacterial strain B-9 has been investigated. Bradykinin, β-endorphin, and [Leu(5)]enkephalin were quickly degraded, with half-lives of <5 min. Somatostatin, substance P, and angiotensin I were degraded relatively smoothly, with half-lives of 10 min to 1 h, whereas oxytocin and insulin were slowly degraded, with half-lives of 1 and 4 days, respectively. Vasopressin was barely degraded, with a half-life of >7 days. Linearized vasopressin, prepared by the reductive cleavage of the disulfide bond followed by alkylation with iodoacetamide, was degraded significantly faster than intact vasopressin, with a half-life of 2.5 h. A loop formed by di... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5544097 Wed, 21 Dec 2011 05:00:00 +0100 5544097 http://www.medworm.com/index.php?rid=5524646&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22173208%26dopt%3DAbstract Authors: Huhn C PMID: 22173208 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5524646 Sat, 17 Dec 2011 05:00:00 +0100 5524646 http://www.medworm.com/index.php?rid=5524639&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179490%26dopt%3DAbstract Authors: Martins A, Daffner LA, Fenech A, McGlinchey C, Strlič M Abstract An innovative approach to date fiber-based gelatin silver prints using near-infrared spectroscopy (NIR) and multivariate analysis is presented. NIR spectra were acquired for 152 film stills printed in the USA between 1914 and 1986, and partial least square (PLS) analysis was used to correlate the spectra with the year the photographs were printed. Principal component analysis and spectral interpretation helped clarify the underlying correlation between the print date and the composition and ageing of the photographic papers. The method was successfully validated with an independent set of 66 film stills printed in the USA, and a prediction error (root mean square error of prediction) of 6 years was achieved... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5524639 Sat, 17 Dec 2011 05:00:00 +0100 5524639 http://www.medworm.com/index.php?rid=5524636&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179491%26dopt%3DAbstract Authors: Kaufmann A Abstract High-resolution mass spectrometry (HRMS), which is used for residue analysis in food, has gained wider acceptance in the last few years. This development is due to the availability of more rugged, sensitive, and selective instrumentation. The benefits provided by HRMS over classical unit-mass-resolution tandem mass spectrometry are considerable. These benefits include the collection of full-scan spectra, which provides greater insight into the composition of a sample. Consequently, the analyst has the freedom to measure compounds without previous compound-specific tuning, the possibility of retrospective data analysis, and the capability of performing structural elucidations of unknown or suspected compounds. HRMS strongly competes with classical tandem... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5524636 Sat, 17 Dec 2011 05:00:00 +0100 5524636 http://www.medworm.com/index.php?rid=5524647&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22173207%26dopt%3DAbstract Authors: Berendsen BJ, Wegh RS, Essers ML, Stolker AA, Weigel S Abstract A liquid chromatography-tandem mass spectrometry method for the analysis of seven antiviral drugs, zanamivir, ribavirin, oseltamivir, oseltamivir carboxylate, amantadine, rimantadine and arbidol, in poultry muscle is reported. The antiviral drugs were extracted from the homogenized poultry muscle sample using methanol. The extract was purified using tandem solid-phase extraction combining a cation exchange cartridge and a phenylboronic acid cartridge. To prevent excessive matrix effects, the analytes were separated from the matrix constituents using a column-switch liquid chromatography system combining a reversed-phase and a Hypercarb analytical column. Detection was carried out using tandem mass spectrometry... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5524647 Fri, 16 Dec 2011 05:00:00 +0100 5524647 http://www.medworm.com/index.php?rid=5524642&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22173209%26dopt%3DAbstract Authors: Lopes Pinheiro SC, Descalzo AB, Raimundo IM, Orellana G, Moreno-Bondi MC Abstract This paper describes the synthesis and characterization of a fluorescent ion-imprinted polymer (IIP) for selective determination of copper ions in aqueous samples. The IIP has been prepared using a novel functional monomer, 4-[(E)-2-(4'-methyl-2,2'-bipyridin-4-yl)vinyl]phenyl methacrylate (abbreviated as BSOMe) that has been spectroscopically characterized in methanolic solution, in the absence and in the presence of several metal ions, including Cd(II), Cu(II), Hg(II), Ni(II), Pb(II), and Zn(II). The stability constant (2.04 × 10(8) mol(-2) l(2)) and stoichiometry (L(2)M) of the BSOMe complex with Cu(II) were extracted thereof. Cu(II)-IIPs were prepared by radical polymerization using... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5524642 Fri, 16 Dec 2011 05:00:00 +0100 5524642 http://www.medworm.com/index.php?rid=5524649&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22169950%26dopt%3DAbstract Authors: Sabbatini L, Torsi L PMID: 22169950 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5524649 Thu, 15 Dec 2011 05:00:00 +0100 5524649 http://www.medworm.com/index.php?rid=5524648&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22169951%26dopt%3DAbstract Authors: Pavan S, Berti F Abstract This review deals with short peptides (up to 50 amino acids) as biomimetic active recognition elements in sensing systems. Peptide-based sensors have been developed in recent years according to different strategies. Synthetic peptides have been designed on the basis of known interactions between single or a few amino acids and targets, with attention being paid to the presence of peptide motifs known to allow intermolecular self-organization of the sensing peptides over the sensor surface. Sensitive and sophisticated sensors have been obtained in this way, but the use of designed peptides is limited by severe difficulties in their in silico design. Short peptides from random phage display have been selected in a random way from large, unfocussed, ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5524648 Thu, 15 Dec 2011 05:00:00 +0100 5524648 http://www.medworm.com/index.php?rid=5505783&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22167314%26dopt%3DAbstract Authors: Oberbeckmann-Winter N PMID: 22167314 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505783 Wed, 14 Dec 2011 05:00:00 +0100 5505783 http://www.medworm.com/index.php?rid=5505807&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159369%26dopt%3DAbstract Authors: Paglia G, Hrafnsdóttir S, Magnúsdóttir M, Fleming RM, Thorlacius S, Palsson BO, Thiele I Abstract Here we present an ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method for extracellular measurements of known and unexpected metabolites in parallel. The method was developed by testing 86 metabolites, including amino acids, organic acids, sugars, purines, pyrimidines, vitamins, and nucleosides, that can be resolved by combining chromatographic and m/z dimensions. Subsequently, a targeted quantitative method was developed for 80 metabolites. The presented method combines a UPLC approach using hydrophilic interaction liquid chromatography (HILIC) and MS detection achieved by a hybrid quadrupole-time of flight (Q-ToF) mass spectrometer. The optimal set... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505807 Tue, 13 Dec 2011 05:00:00 +0100 5505807 http://www.medworm.com/index.php?rid=5505805&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159424%26dopt%3DAbstract Authors: Zeng X, Shen Z, Mernaugh R Abstract Inexpensive, noninvasive immunoassays can be used to quickly detect disease in humans. Immunoassay sensitivity and specificity are decidedly dependent upon high-affinity, antigen-specific antibodies. Antibodies are produced biologically. As such, antibody quality and suitability for use in immunoassays cannot be readily determined or controlled by human intervention. However, the process through which high-quality antibodies can be obtained has been shortened and streamlined by use of genetic engineering and recombinant antibody techniques. Antibodies that traditionally take several months or more to produce when animals are used can now be developed in a few weeks as recombinant antibodies produced in bacteria, yeast, or other cell type... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505805 Tue, 13 Dec 2011 05:00:00 +0100 5505805 http://www.medworm.com/index.php?rid=5505804&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159425%26dopt%3DAbstract In this study, we applied the IMS technique to formalin-fixed paraffin-embedded samples to identify a substance(s) responsible for the intestinal obstruction caused by an unidentified foreign body. In advance of IMS analysis, some pretreatments were applied. After the deparaffinization of sections, samples were subjected to enzyme digestion. The sections co-crystallized with matrix were desorbed and ionized by a laser pulse with scanning. A combination of α-amylase digestion and the 2,5-dihydroxybenzoic acid matrix gave the best mass spectrum. With the IMS Convolution software which we developed, we could automatically extract meaningful signals from the IMS datasets. The representative peak values were m/z 1,013, 1,175, 1,337, 1,499, 1,661, 1,823, and 1,985. Thus, it was revealed that th... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505804 Tue, 13 Dec 2011 05:00:00 +0100 5505804 http://www.medworm.com/index.php?rid=5505806&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159370%26dopt%3DAbstract Authors: Armenta S, Blanco M Abstract The capabilities of ion mobility spectrometry (IMS) as a high throughput and green analytical tool in the occupational health and safety control, using pyrethroids as models has been evidenced. The method used for dermal and inhalation exposure assessment is based on the passive pyrethroid sampling using Teflon membranes, direct thermal extraction of the pyrethroids, and measurement of the vaporized analytes by IMS without reagent and solvent consumption. The IMS signatures of the studied synthetic pyrethroids under atmospheric pressure chemical ionization by investigating the formed negative ion products have been obtained. The main advantages of the proposed procedure are related to the obtained limits of detection, ranging from 0.08 to 5 ng... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505806 Mon, 12 Dec 2011 05:00:00 +0100 5505806 http://www.medworm.com/index.php?rid=5505784&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160237%26dopt%3DAbstract In conclusion, this method exhibits good analytical performances and meets the criteria of sensitivity suitable for HCit concentration assessment in plasma samples. PMID: 22160237 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505784 Mon, 12 Dec 2011 05:00:00 +0100 5505784 http://www.medworm.com/index.php?rid=5505787&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160206%26dopt%3DAbstract Authors: Lavilla M, Moros M, Puertas S, Grazú V, Pérez MD, Calvo M, de la Fuente JM, Sánchez L Abstract Nowadays, the reference method for the detection of Clostridium tyrobutyricum in milk is the most-probable-number method, a very time-consuming and non-specific method. In this work, the suitability of the use of superparamagnetic beads coated with specific antibodies and peptides for bioseparation and concentration of spores of C. tyrobutyricum has been assessed. Peptide or antibody functionalized nanoparticles were able to specifically bind C. tyrobutyricum spores and concentrate them up to detectable levels. Moreover, several factors, such as particle size (200 nm and 1 μm), particle derivatization (aminated and carboxylated beads), coating method, and type of ligand hav... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505787 Sun, 11 Dec 2011 05:00:00 +0100 5505787 http://www.medworm.com/index.php?rid=5505786&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160235%26dopt%3DAbstract We present a microfluidic system that facilitates long-term measurements of single cell response to external stimuli. The difficulty of addressing cells individually was overcome by using a two-layer microfluidic device. The top layer is designed for trapping and culturing of cells while the bottom layer is employed for supplying chemical compounds that can be transported towards the cells in defined concentrations and temporal sequences. A porous polyester membrane that supports transport and diffusion of compounds from below separates the microchannels of both layers. The performance and potential of the device are demonstrated using human embryonic kidney cells (HEK293) transfected with an inducible gene expression system. Expression of a fluorescent protein (ZsGreen1-DR) is observed wh... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505786 Sun, 11 Dec 2011 05:00:00 +0100 5505786 http://www.medworm.com/index.php?rid=5505785&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160236%26dopt%3DAbstract Authors: Pisonero J, Valledor R, Licciardello A, Quirós C, Martín JI, Sanz-Medel A, Bordel N Abstract Direct solid analysis of ultrathin layers is investigated using pulsed radiofrequency (rf) glow discharge (GD) time-of-flight mass spectrometry (TOFMS). In particular, previous studies have always integrated the detected ion signals in the afterglow region of the rf-GD pulse, which is known to be the most sensitive one. Nevertheless, the analytical capabilities of other pulse time regions have not been evaluated in detail. Therefore, in this work, we investigate the analyte prepeak region, which is the pulse region where the analyte ions peak after the initial sputtering process of each GD pulse, aiming at obtaining improved depth profile analysis with high depth resolution and w... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505785 Sun, 11 Dec 2011 05:00:00 +0100 5505785 http://www.medworm.com/index.php?rid=5505791&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160202%26dopt%3DAbstract In this study, S. divinorum was extracted and spiked onto four different plant materials (S. divinorum, Salvia officinalis, Cannabis sativa, and Nicotiana tabacum) to simulate an adulterated sample that might be encountered in a forensic laboratory. The adulterated samples were extracted and analyzed by gas chromatography-mass spectrometry, and the resulting total ion chromatograms were subjected to a series of pretreatment procedures that were used to minimize non-chemical sources of variance in the data set. The data were then analyzed using principal components analysis (PCA) to investigate association of the adulterated extracts to unadulterated S. divinorum. While association was possible based on visual assessment of the PCA scores plot, additional procedures including Euclidean dist... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505791 Sat, 10 Dec 2011 05:00:00 +0100 5505791 http://www.medworm.com/index.php?rid=5505790&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160203%26dopt%3DAbstract Authors: Mazumder A, Kumar A, Purohit AK, Dubey DK Abstract A high-resolution phosphorus-31 nuclear magnetic resonance (NMR) spectroscopic method has been developed for detection, identification and quantification of non-phosphorus markers of toxic nerve agents (soman and V-class), vesicants (HD, HN-2, HN-3), and incapacitating agent (Bz). These analytes were converted to phosphorus-containing derivatives via phosphitylation reaction of their hydroxyl and sulfhydryl functions (using 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane). This was followed by (31)P{(1)H} and (31)P NMR analysis of these derivatives. The chemical shifts (δ) and coupling constants ((3) J (P-H)) of derivatives were used for their specific detection and identification. The method allow... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505790 Sat, 10 Dec 2011 05:00:00 +0100 5505790 http://www.medworm.com/index.php?rid=5505789&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160204%26dopt%3DAbstract Authors: Nichkova MI, Huisman H, Wynveen PM, Marc DT, Olson KL, Kellermann GH Abstract Depression is a common disorder with physical and psychological manifestations often associated with low serotonin. Since noninvasive diagnostic tools for depression are sparse, we evaluated the clinical utility of a novel ELISA for the measurement of serotonin in urine from depressed subjects and from subjects under antidepressant therapy. We developed a competitive ELISA for direct measurement of serotonin in derivatized urine samples. Assay performance was evaluated and applied to clinical samples. The analytical range of the assay was from 6.7 to 425 μg serotonin/g creatinine (Cr). The limit of quantification was 4.7 μg/g Cr. The average recovery for spiked urine samples was 104.4%. Avera... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505789 Sat, 10 Dec 2011 05:00:00 +0100 5505789 http://www.medworm.com/index.php?rid=5505788&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160205%26dopt%3DAbstract Authors: Moser C, Zoderer D, Luef G, Rauchenzauner M, Wildt L, Griesmacher A, Seger C Abstract Co-administration of synthetic progestin containing hormonal contraceptives (HCs) and antiepileptic drugs (AEDs) is a common clinical situation which needs specific considerations due to drug interactions. Several studies have demonstrated that lamotrigine plasma levels are significantly decreased during co-medication with HCs, and that this interaction is associated with increased seizure frequency in most of the cases. Additionally, an increase in contraceptive failure and unintended pregnancy could be observed during co-medication. Hence, monitoring of progestin plasma levels in patients with AED co-medication is of interest. A rapid and reliable online solid-phase extraction-high perf... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505788 Sat, 10 Dec 2011 05:00:00 +0100 5505788 http://www.medworm.com/index.php?rid=5505800&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159464%26dopt%3DAbstract Authors: Hahn O PMID: 22159464 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505800 Fri, 09 Dec 2011 05:00:00 +0100 5505800 http://www.medworm.com/index.php?rid=5505794&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159512%26dopt%3DAbstract Authors: Narukawa T, Inagaki K, Zhu Y, Kuroiwa T, Narushima I, Chiba K, Hioki A Abstract A certified reference material, NMIJ CRM 7405-a, for the determination of trace elements and As(V) in algae was developed from the edible marine hijiki (Hizikia fusiforme) and certified by the National Metrology Institute of Japan (NMIJ), the National Institute of Advanced Industrial Science and Technology (AIST). Hijiki was collected from the Pacific coast in the Kanto area of Japan, and was washed, dried, powdered, and homogenized. The hijiki powder was placed in 400 bottles (ca. 20 g each). The concentrations of 18 trace elements and As(V) were determined by two to four independent analytical techniques, including (ID)ICP-(HR)MS, ICP-OES, GFAAS, and HPLC-ICP-MS using calibration solutions p... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505794 Fri, 09 Dec 2011 05:00:00 +0100 5505794 http://www.medworm.com/index.php?rid=5505793&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159513%26dopt%3DAbstract Authors: Detwiler DA, Dobes NC, Sims CE, Kornegay JN, Allbritton NL Abstract Despite identification of a large number of adult stem cell types, current primary cell isolation and identification techniques yield heterogeneous samples, making detailed biological studies challenging. To identify subsets of isolated cells, technologies capable of simultaneous cell culture and cloning are necessary. Micropallet arrays, a new cloning platform for adherent cell types, hold great potential. However, the microstructures composing these arrays are fabricated from an epoxy photoresist 1002F, a growth surface unsuitable for many cell types. Optimization of the microstructures' surface properties was conducted for the culture of satellite cells, primary muscle cells for which improved cell isol... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505793 Fri, 09 Dec 2011 05:00:00 +0100 5505793 http://www.medworm.com/index.php?rid=5505792&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159514%26dopt%3DAbstract Authors: Marosi A, Szalay Z, Béni S, Szakács Z, Gáti T, Rácz A, Noszál B, Demeter A Abstract Multinuclear one (1D-) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopic investigations of famotidine, the most potent and widely used histamine H(2)-receptor antagonist, were carried out in dimethyl sulfoxide-d(6) (DMSO-d(6)) and water. Previous NMR assignments were either incomplete or full assignment was based only on 1D spectra and quantum-chemical calculations. Our work revealed several literature misassignments of the (1)H, (13)C, and (15)N NMR signals and clarified the acid-base properties of the compound at the site-specific level. The erroneous assignment of Baranska et al. (J. Mol. Struct. 2001, 563) probably originates from an incorrect hypothesis about... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505792 Fri, 09 Dec 2011 05:00:00 +0100 5505792 http://www.medworm.com/index.php?rid=5505803&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159461%26dopt%3DAbstract Authors: Huang K, Martí AA Abstract A molecular beacon (MB) is a hairpin-structured oligonucleotide probe containing a photoluminescent species (PLS) and a quencher at different ends of the strand. In a recognition and detection process, the hybridization of MBs with target DNA sequences restores the strong photoluminescence, which is quenched before hybridization. Making better MBs involves reducing the background photoluminescence and increasing the brightness of the PLS, which therefore involves the development of new PLS and quenchers, as well as innovative PLS-quencher systems. Heavy-metal complexes, nanocrystals, pyrene compounds, and other materials with excellent photophysical properties have been applied as PLS of MBs. Nanoparticles, nanowires, graphene, metal films, and ... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505803 Thu, 08 Dec 2011 05:00:00 +0100 5505803 http://www.medworm.com/index.php?rid=5505802&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159462%26dopt%3DAbstract Authors: Król S, Zabiegała B, Namieśnik J Abstract The concentration of benzene in urban air in the Tri-City area of Poland (Gdańsk-Sopot-Gdynia, and Tczew) was assessed using diffusive passive samplers (Radiello). Samples were collected during a four-year monitoring campaign (2007-2010) at selected monitoring stations managed by the Agency of Regional Air Quality Monitoring in the Gdańsk Metropolitan Area (ARMAAG) Foundation. The performance of the passive samplers was investigated in a field study that measured the benzene concentration in urban air. The results obtained by the Radiello samplers were compared with the results obtained using an on-line monitor (Chrompack CP 7001). Statistical analysis of the results obtained by the two different techniques (passive and on-lin... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505802 Thu, 08 Dec 2011 05:00:00 +0100 5505802 http://www.medworm.com/index.php?rid=5505801&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159463%26dopt%3DAbstract Authors: Carey AM, Lombi E, Donner E, de Jonge MD, Punshon T, Jackson BP, Guerinot ML, Price AH, Meharg AA Abstract Rice is a staple food yet is a significant dietary source of inorganic arsenic, a class 1, nonthreshold carcinogen. Establishing the location and speciation of arsenic within the edible rice grain is essential for understanding the risk and for developing effective strategies to reduce grain arsenic concentrations. Conversely, selenium is an essential micronutrient and up to 1 billion people worldwide are selenium-deficient. Several studies have suggested that selenium supplementation can reduce the risk of some cancers, generating substantial interest in biofortifying rice. Knowledge of selenium location and speciation is important, because the anti-cancer effects of... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505801 Thu, 08 Dec 2011 05:00:00 +0100 5505801 http://www.medworm.com/index.php?rid=5505799&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159465%26dopt%3DAbstract Authors: Wei X, Li H, Li Z, Vuki M, Fan Y, Zhong W, Xu D Abstract In this paper, a novel metal plasmon coupled with an aptamer-nucleotide hybridized probe was fabricated and applied for protein detection. The specific aptamer and single-strand oligonucleotide were chemically bound to silver nanoparticles (AgNPs), and Cy5-labeled, complementary single-strand oligonucleotides were hybridized with the particle-bound oligonucleotides. The hybridized DNA duplexes were regarded as rigid rods that separated the fluorophore Cy5 and the surface of AgNPs to reduce the competitive quenching. Using a model system comprising human immunoglobulin E (IgE) as the analyte and goat antihuman IgE as immobilized capture antibody on glass slides, we demonstrate that the detection performance of the syn... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505799 Thu, 08 Dec 2011 05:00:00 +0100 5505799 http://www.medworm.com/index.php?rid=5505798&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159466%26dopt%3DAbstract Authors: Kolarov F, Niedergall K, Bach M, Tovar GE, Gauglitz G Abstract Molecularly imprinted nanospheres obtained by miniemulsion polymerization have been applied as the sensitive layer for label-free direct optical sensing of small molecules. Using these particles as the sensitive layer allowed for improving response times in comparison to sensors using MIP layers. As a model compound, well-characterized nanospheres imprinted against L: -Boc-phenylalanine anilide (L: -BFA) were chosen. For immobilization, a simple concept based on electrostatic adsorption was used, showing its applicability to different types of surfaces, leading to a good surface coverage. The sensor showed short response times, good selectivity, and high reversibility with a limit of detection down to 60 μM a... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505798 Thu, 08 Dec 2011 05:00:00 +0100 5505798 http://www.medworm.com/index.php?rid=5505797&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159467%26dopt%3DAbstract Authors: Wagner B, Nowak A, Bulska E, Hametner K, Günther D Abstract Corning archeological reference glasses A, B, C, and D have been made to simulate different historic technologies of glass production and are used as standards in historic glass investigations. In this work, nanoseconds (193, 266 nm) and femtosecond (800 nm) laser ablation were used to study the elemental composition of Corning glasses using laser ablation inductively coupled plasma mass spectrometry. The determined concentrations of 26 oxides (Li(2)O, B(2)O(3), Na(2)O, MgO, Al(2)O(3), SiO(2), P(2)O(5), K(2)O, CaO, TiO(2), V(2)O(5), Cr(2)O(3), MnO, Fe(2)O(3), CoO, NiO, CuO, ZnO, Rb(2)O, SrO, ZrO(2), SnO(2), Sb(2)O(5), BaO, PbO, Bi(2)O(3)) are compared with values reported in the literature. Results show variabl... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505797 Thu, 08 Dec 2011 05:00:00 +0100 5505797 http://www.medworm.com/index.php?rid=5505796&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159468%26dopt%3DAbstract Authors: Matsumiya H, Kitakata K, Hiraide M Abstract The analysis of seawater for trace metals is important for pollution monitoring and better understanding of marine systems. The present paper describes an efficient preconcentration method for the determination of trace metals in seawater. Trace metals [Ni(II), Cu(II), Ga(III), Cd(II), Pb(II), and Bi(III)] in 1,000 mL of seawater sample were complexed with ammonium pyrrolidinedithiocarbamate and sorbed onto silica particles covered with cetyltrimethylammonium chloride. After the addition of sodium dodecyl sulfate, the particles were floated to the solution surface by bubbling and then collected by suction. The trace metals were desorbed with dilute nitric acid and determined by inductively coupled plasma-mass spectrometry. The r... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505796 Thu, 08 Dec 2011 05:00:00 +0100 5505796 http://www.medworm.com/index.php?rid=5505795&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22159469%26dopt%3DAbstract Authors: Dahlin AP, Hjort K, Hillered L, Sjödin MO, Bergquist J, Wetterhall M Abstract A simple and straightforward method for discovery and quantification of proteins adsorbed onto delicate and sensitive membrane surfaces is presented. The adsorbed proteins were enzymatically cleaved while still adsorbed onto the membranes using an on-surface enzymatic digestion (oSED). This was followed by isobaric tagging, nanoliquid chromatography, and tandem mass spectrometry. Protein adsorption on tri-block copolymer Poloxamer 407 surface-modified microdialysis (MD) membranes were compared with protein adsorption on unmodified MD membranes. Ventricular cerebrospinal fluid (vCSF) kept at 37 °C was used as sample matrix. In total, 19 proteins were quantified in two biological replicates. The... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505795 Thu, 08 Dec 2011 05:00:00 +0100 5505795 http://www.medworm.com/index.php?rid=5505811&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22147270%26dopt%3DAbstract We report a sample pretreatment approach for the analysis of total cocaine residues in wastewater that eliminates the need for two key assumptions often made in estimating cocaine utilization from measurement of its benzoylecgonine metabolite: that benzoylecgonine is neither degraded nor generated during transport in a sewer system, and that it is excreted as a constant fraction of cocaine ingested. By adding NaOH and incubating samples at 55 °C, cocaine and its principal metabolites are efficiently hydrolyzed into ecgonine, anhydroecgonine, and norecgonine. Ecgonine, estimated to represent between 37% and 90% (on a molar basis) of cocaine residues, can be directly determined (without preconcentration via solid-phase extraction (SPE)) by reversed-phase (RP) or hydrophilic interaction liq... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505811 Wed, 07 Dec 2011 05:00:00 +0100 5505811 http://www.medworm.com/index.php?rid=5505810&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22147271%26dopt%3DAbstract Authors: Walgraeve C, Demeestere K, De Wispelaere P, Dewulf J, Lintelmann J, Fischer K, Van Langenhove H Abstract An innovative analytical method based on high-performance liquid chromatography and atmospheric pressure chemical ionization magnetic sector mass spectrometry was developed and optimized to determine trace concentrations of 11 compounds belonging to the group of the seldom-analyzed oxy-PAHs (phenanthrene-9,10-dione, chrysene-5,6-dione, benzo[a]pyrene-4,5-dione, benzo[a]pyrene-1,6-dione, benzo[a]pyrene-3,6-dione, benzo[a]pyrene-6,12-dione, 4-oxa-benzo[def]chrysene-5-one, pyrene-1-carboxaldehyde, benzo[de]anthracene-7-one, benzo[a]anthracene-7,12-dione, and napthacene-5,12-dione) on airborne particulate matter (PM(10)). The mass spectrometer was operated in multiple ion d... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505810 Wed, 07 Dec 2011 05:00:00 +0100 5505810 http://www.medworm.com/index.php?rid=5505809&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22147272%26dopt%3DAbstract In this study, we examined a simple preparation and highly sensitive analysis of drugs in biological samples such as urine, plasma, and organs using thin-layer chromatography coupled with matrix-assisted laser desorption/ionization mass spectrometry (TLC/MALDI/MS). When the urine containing 3,4-methylenedioxymethamphetamine (MDMA) without sample dilution was spotted on a thin-layer chromatography (TLC) plate and was analyzed by TLC/MALDI/MS, the detection limit of the MDMA spot was 0.05 ng/spot. The value was the same as that in aqueous solution spotted on a stainless steel plate. All the 11 psychotropic compounds tested (MDMA, 4-hydroxy-3-methoxymethamphetamine, 3,4-methylenedioxyamphetamine, methamphetamine, p-hydroxymethamphetamine, amphetamine, ketamine, caffeine, chlorpromazine, tria... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505809 Wed, 07 Dec 2011 05:00:00 +0100 5505809 http://www.medworm.com/index.php?rid=5505808&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22147273%26dopt%3DAbstract In conclusion, the qualitative PCR system was found to be a relatively inexpensive, rapid, and flexible method of identifying the porcine origin of and ruminant contaminants in ICPH, while the quantitative PCR was found suitable to accurately analyze the components and contaminants in detail. Both methods are suitable for routine control assays for the evaluation of ICPH purity and origins of contaminants. PMID: 22147273 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry) Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505808 Wed, 07 Dec 2011 05:00:00 +0100 5505808 http://www.medworm.com/index.php?rid=5505812&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22143743%26dopt%3DAbstract Authors: Cordero C, Zebelo SA, Gnavi G, Griglione A, Bicchi C, Maffei ME, Rubiolo P Abstract Headspace solid-phase microextraction (HS-SPME) comprehensive two-dimensional (2D) gas chromatography combined with quadrupole-mass spectrometry (GC×GC-qMS) with dedicated comparative data elaboration was applied to separate chemical patterns arising from the interaction between some Mentha species and the herbivore Chrysolina herbacea, also known as the mint bug. Upon feeding on different Mentha species (Mentha spicata L., Mentha × piperita L. and Mentha longifolia L.), C. herbacea produced frass (faeces) which were characterized by a typical volatile fraction. HS-SPME GC×GC-qMS analysis of the complex volatile fraction of both mint leaf and C. herbacea frass was submitted to advanc... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505812 Tue, 06 Dec 2011 05:00:00 +0100 5505812 http://www.medworm.com/index.php?rid=5505814&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22139524%26dopt%3DAbstract Authors: Yang CY, Ding WH Abstract A simple and solvent-minimized procedure for the determination of six commonly found synthetic polycyclic musks in aqueous samples using ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) coupled with gas chromatography-mass spectrometry (GC-MS) is described. The parameters affecting the extraction efficiency of analytes from water samples were systematically investigated. The best extraction conditions involved the rapid injection of a mixture of 1.0 mL of isopropyl alcohol (as a dispersant) and 10 μL of carbon tetrachloride (as an extractant) into 10 mL of water containing 0.5 g of sodium chloride in a conical-bottom glass tube. After ultrasonication for 1.0 min and centrifugation at 5,000 rpm (10 min), the sedimente... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505814 Sun, 04 Dec 2011 05:00:00 +0100 5505814 http://www.medworm.com/index.php?rid=5505813&cid=s_37498_59_f&fid=37498&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22139525%26dopt%3DAbstract In this study, a full tertiary structure model of the rProG molecule of 198 amino acid residues containing a signal region, two IgG binding domains, and an anchor region, was computationally generated using the iterative threading assembly refinement (I-Tasser) server. The rProG model having the highest confidence score was subject to docking experiments with varied-length short chains of PMMA polymer via the graphic processing units-based Hex server. A five-residue section of the rProG anchor region, with the sequence TPATP, was identified as a potential interaction site. A complete ternary model (rProG, PMMA, and IgG) was assembled and provides insight into a plausible mechanism for non-covalent antibody orientation by the ALYGNSA system. PMID: 22139525 [PubMed - as supplied by publi... Analytical and Bioanalytical Chemistry journals http://www.medworm.com/rss/comments.php?id=5505813 Sun, 04 Dec 2011 05:00:00 +0100 5505813