MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Angiogenesis' source. Mon, 06 Feb 2012 16:52:00 +0100 http://www.medworm.com/index.php?rid=5664290&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fdq1451519r56l403%2F Abstract  Pharmacological inhibition of VEGF-A has proven to be effective in inhibiting angiogenesis and vascular leak associated with cancers and various eye diseases. However, little information is currently available on the binding kinetics and relative biological activity of various VEGF inhibitors. Therefore, we have evaluated the binding kinetics of two anti-VEGF antibodies, ranibizumab and bevacizumab, and VEGF Trap (also known as aflibercept), a novel type of soluble decoy receptor, with substantially higher affinity than conventional soluble VEGF receptors. VEGF Trap bound to all isoforms of human VEGF-A tested with subpicomolar affinity. Ranibizumab and bevacizumab also bound human VEGF-A, but with markedly lower affinity. The association rate for VEGF Trap bindin... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5664290 Thu, 02 Feb 2012 18:09:18 +0100 5664290 http://www.medworm.com/index.php?rid=5617048&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr022156022976826%2F Abstract  The functional shift of quiescent endothelial cells into tip cells that migrate and stalk cells that proliferate is a key event during sprouting angiogenesis. We previously showed that the sialomucin CD34 is expressed in a small subset of cultured endothelial cells and that these cells extend filopodia: a hallmark of tip cells in vivo. In the present study, we characterized endothelial cells expressing CD34 in endothelial monolayers in vitro. We found that CD34-positive human umbilical vein endothelial cells show low proliferation activity and increased mRNA expression of all known tip cell markers, as compared to CD34-negative cells. Genome-wide mRNA profiling analysis of CD34-positive endothelial cells demonstrated enrichment for biological functions related to ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5617048 Tue, 17 Jan 2012 07:18:44 +0100 5617048 http://www.medworm.com/index.php?rid=5568910&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6820n43h52080430%2F Conclusions  The schedule of neoadjuvant therapy tested was safe and active. Pre-treatment vessel density by the panendothelial marker anti CD-34 antibody, post-treatment Ki-67 labeling index and VEGFR-2 expression were significantly associated to residual tumor area. The biomarkers correlations warrant further evaluation in prospective clinical trials. Content Type Journal ArticleCategory Original PaperPages 1-10DOI 10.1007/s10456-011-9250-0Authors Giampietro Gasparini, Unità Operativa Complessa di Oncologia Medica, Azienda Complesso Ospedaliero di Rilevanza Nazionale “S. Filippo Neri”, Via G. Martinotti, 20, 00135 Rome, ItalyFrancesco Torino, Unità Operativa Complessa di Oncologia Medica, Azienda Complesso Ospedaliero di Rilevanza Nazionale “S. Filippo Neri... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5568910 Mon, 02 Jan 2012 16:46:32 +0100 5568910 http://www.medworm.com/index.php?rid=5568911&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx2u55j722087632u%2F Abstract  Persistent inflammation and neovascularization are critical to cancer development. In addition to upregulation of positive control mechanisms such as overexpression of angiogenic and inflammatory factors in the cancer microenvironment, loss of otherwise normally functioning negative control mechanisms is likely to be an important attribute. Insights into the down-modulation of such negative control mechanisms remain largely unclear, however. We show here that tumor necrosis factor superfamily-15 (TNFSF15), an endogenous inhibitor of neovascularization, is a critical component of the negative control mechanism that operates in normal ovary but is missing in ovarian cancer. We show in clinical settings that TNFSF15 is present prominently in the vasculature of normal... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5568911 Fri, 30 Dec 2011 16:50:14 +0100 5568911 http://www.medworm.com/index.php?rid=5556789&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx6134j4p07500m32%2F Abstract  Exosomes, microvesicles of endocytic origin released by normal and tumor cells, play an important role in cell-to-cell communication. Angiogenesis has been shown to regulate progression of chronic myeloid leukemia (CML). The mechanism through which this happens has not been elucidated. We isolated and characterized exosomes from K562 CML cells and evaluated their effects on human umbilical endothelial cells (HUVECs). Fluorescent-labeled exosomes were internalized by HUVECs during tubular differentiation on Matrigel. Exosome localization was perinuclear early in differentiation, moving peripherally in cells undergoing elongation and connection. Exosomes move within and between nanotubular structures connecting the remodeling endothelial cells. They stimulated angiot... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5556789 Tue, 27 Dec 2011 17:01:16 +0100 5556789 http://www.medworm.com/index.php?rid=5556790&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6w6k12782252l156%2F Abstract  Angiogenesis is important for tumor growth and metastasis. CLT1 (CGLIIQKNEC), a peptide that binds to tumor interstitial spaces in the presence of fibrin-fibronectin, has structural similarity to the anti-angiogenic β-sheet peptides anastellin and anginex. This similarity is reflected in the ability of CLT1 to form co-aggregates with fibronectin that induce an unfolded protein response and cause autophagic cell death in proliferating endothelial cells. CLT1 cytotoxicity is mediated at least in parts by a novel CLT1 binding protein, Chloride Intracellular Channel 1 (CLIC1), which promotes internalization of CLT1-fibronectin co-aggregates in a mechanism that depends on the LIIQK amino acid sequence of CLT1. LIIQK encompasses amino acid residues relevant for CLT1 bi... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5556790 Tue, 27 Dec 2011 17:01:14 +0100 5556790 http://www.medworm.com/index.php?rid=5549804&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn52061352244545w%2F Abstract  Small cell lung cancer (SCLC) demonstrates a strong etiological association with smoking. Although cigarette smoke is a mixture of about 4,000 compounds, nicotine is the addictive component of cigarette smoke. Several convergent studies have shown that nicotine promotes angiogenesis in lung cancers via the α7-nicotinic acetylcholine receptor (α7-nAChR) on endothelial cells. Therefore, we conjectured that α7-nAChR antagonists may attenuate nicotine-induced angiogenesis and be useful for the treatment of human SCLC. For the first time, our study explores the anti-angiogenic activity of MG624, a small-molecule α7-nAChR antagonist, in several experimental models of angiogenesis. We observed that MG624 potently suppressed the proliferation of primary human microvas... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5549804 Sat, 24 Dec 2011 16:45:49 +0100 5549804 http://www.medworm.com/index.php?rid=5549805&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F65002252t6111418%2F Conclusion  Our observations reveal possible mechanisms for the inhibitory effects of bleomycin on hemangiomagenesis, and raise the possibility that bcl-2 might be an important therapeutic target in the treatment of hemangiomas. Content Type Journal ArticleCategory Original PaperPages 1-9DOI 10.1007/s10456-011-9248-7Authors Peace Mabeta, Department of Physiology, Faculty of Health Sciences, University of Pretoria, P.O. Box 2034, Pretoria, 0001 South AfricaMichael S. Pepper, Department of Immunology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5549805 Sat, 24 Dec 2011 16:45:48 +0100 5549805 http://www.medworm.com/index.php?rid=5542220&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn4n12426103857m8%2F In this study, we demonstrate the utility of LSCI for characterizing microvascular remodeling and hemodynamic changes during wound healing angiogenesis in vivo. A 2 mm diameter hole was made in the mouse ear and the periphery of the wound imaged in vivo using LSCI over 12 days. We were able to visualize and quantify the vascular and perfusion changes that accompanied wound healing in the microenvironment proximal to the wound, and validated these changes with histology. We found that consistent with the stages of wound healing, microvessel density increased during the initial inflammatory phase (i.e., day 0–3), stayed elevated through the tissue formation phase (i.e., until day 7) and returned to baseline during the tissue remodeling phase (i.e., by day 12). Concomitant �... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5542220 Fri, 23 Dec 2011 16:54:18 +0100 5542220 http://www.medworm.com/index.php?rid=5542221&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx75374nx11414886%2F This study provides a compelling rationale for clinical investigation in patients with advanced HCC. Content Type Journal ArticleCategory Original PaperPages 1-12DOI 10.1007/s10456-011-9243-zAuthors Hung Huynh, Laboratory of Molecular Endocrinology, Division of Molecular and Cellular Research, National Cancer Centre, 11 Hospital Drive, Singapore, 169610 SingaporeRichard Ong, Laboratory of Molecular Endocrinology, Division of Molecular and Cellular Research, National Cancer Centre, 11 Hospital Drive, Singapore, 169610 SingaporeKhee Chee Soo, Laboratory of Molecular Endocrinology, Division of Molecular and Cellular Research, National Cancer Centre, 11 Hospital Drive, Singapore, 169610 Singapore Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogen... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5542221 Tue, 20 Dec 2011 16:45:06 +0100 5542221 http://www.medworm.com/index.php?rid=5533470&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk137081217458v22%2F Abstract  Evidence has accumulated to suggest that magnesium might play a role in controlling angiogenesis. Since microvascular endothelial cells are protagonists in this process, we investigated the behavior of these cells cultured in low extracellular magnesium or silenced for its transporter Transient Receptor Potential Melastatin (TRPM)7, essential for cellular magnesium homeostasis. In particular, we focused on some crucial steps of the angiogenic process, i.e. proliferation, migration, protease production and organization in tridimensional structures. Silencing TRPM7 mimics the effects of low extracellular magnesium on human microvascular endothelial cells (HMEC). Indeed, while no effects were observed on the production of metalloproteases and on tridimensional organi... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5533470 Mon, 19 Dec 2011 16:53:33 +0100 5533470 http://www.medworm.com/index.php?rid=5465217&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F35042201m466234r%2F Abstract  Therapeutic angiogenesis is based on the premise that the development of new blood vessels can be augmented by exogenous administration of the appropriate growth factors. Over the last years, successful preclinical studies and promising results of early clinical trials have created great excitement about the potential of therapeutic angiogenesis for patients with advanced ischemic heart disease. The authors provide an overview of the biology of angiogenesis, the basic characteristics of angiogenic factors, and the different routes of their delivery. They discuss experimental studies in animal models of myocardial ischemia and outline available clinical studies on therapeutic angiogenesis for myocardial ischemia. Related safety issues are also addressed followed by... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5465217 Sat, 26 Nov 2011 16:48:04 +0100 5465217 http://www.medworm.com/index.php?rid=5396835&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp74655801g250054%2F Abstract  There has been a long controversy as to whether interleukin-17 (IL17) has an impact on tumor growth. In order to assess whether IL17 may affect tumor growth, it would be convenient to achieve high levels of this pro-inflammatory cytokine at the tumor neo-vasculature, since IL17 is known to promote angiogenesis. Here, we have generated and tested in vivo a fusion protein, consisting of the F8 antibody (specific to the alternatively spliced EDA domain of fibronectin, a marker of angiogenesis) and of murine IL17 (mIL17). The resulting immunocytokine (termed F8-mIL17) was shown to selectively localize at the tumor neo-vasculature and to vigorously promote tumor angiogenesis, without however reducing or enhancing tumor growth rate both in immunocompetent and in immunod... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5396835 Thu, 03 Nov 2011 16:56:26 +0100 5396835 http://www.medworm.com/index.php?rid=5375466&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm425638237731533%2F Abstract  The G protein-coupled receptor GPR4 is activated by acidic pH and recent evidence indicates that it is expressed in endothelial cells. In agreement with these reports, we observe a high correlation of GPR4 mRNA expression with endothelial marker genes, and we confirm expression and acidic pH dependent function of GPR4 in primary human vascular endothelial cells. GPR4-deficient mice were generated; these are viable and fertile and show no gross abnormalities. However, these animals show a significantly reduced angiogenic response to VEGF (vascular endothelial growth factor), but not to bFGF (basic fibroblast growth factor), in a growth factor implant model. Accordingly, in two different orthotopic models, tumor growth is strongly reduced in mice lacking GPR4. Histo... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5375466 Tue, 01 Nov 2011 16:52:51 +0100 5375466 http://www.medworm.com/index.php?rid=5310786&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4240213j201r70q1%2F Conclusions  The effect of prednisolone therapy for some women with RM may be due to altered endometrial angiogenic growth factor expression and reduced blood vessel maturation. Content Type Journal ArticleCategory Original PaperPages 1-10DOI 10.1007/s10456-011-9237-xAuthors Gendie E. Lash, Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, 3rd Floor, William Leech Building, Newcastle upon Tyne, NE2 4HH UKJudith N. Bulmer, Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, 3rd Floor, William Leech Building, Newcastle upon Tyne, NE2 4HH UKBarbara A. Innes, Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, 3rd Floor, William Leech Building... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5310786 Sat, 08 Oct 2011 15:42:15 +0100 5310786 http://www.medworm.com/index.php?rid=5301843&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr762472600g21107%2F Abstract  Growth of the remaining lung after pneumonectomy has been observed in many mammalian species; nonetheless, the pattern and morphology of alveolar angiogenesis during compensatory growth is unknown. Here, we investigated alveolar angiogenesis in a murine model of post-pneumonectomy lung growth. As expected, the volume and weight of the remaining lung returned to near-baseline levels within 21 days of pneumonectomy. The percentage increase in lobar weight was greatest in the cardiac lobe (P < 0.001). Cell cycle flow cytometry demonstrated a peak of lung cell proliferation (12.02 ± 1.48%) 6 days after pneumonectomy. Spatial autocorrelation analysis of the cardiac lobe demonstrated clustering of similar vascular densities (positive auto... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5301843 Tue, 04 Oct 2011 05:52:09 +0100 5301843 http://www.medworm.com/index.php?rid=5292959&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fbr61v73784831252%2F Abstract  Hypoxia-inducible factor (HIF-1) is the key transcription regulator for multiple angiogenic factors and is an appealing target. Ginsenoside-Rg1, a nontoxic saponin isolated from the rhizome of Panax ginseng, exhibits potent proangiogenic activity and has the potential to be developed as a new angiotherapeutic agent. However, the mechanisms by which Rg1 promotes angiogenesis are not fully understood. Here, we show that Rg1 is an effective stimulator of HIF-1α under normal cellular oxygen conditions in human umbilical vein endothelial cells. HIF-1α steady-state mRNA was not affected by Rg1. Rather, HIF-1α protein synthesis was stimulated by Rg1. This effect was associated with constitutive activation of phosphatidylinositol 3-kinase (PI3K)/Akt and its effector p70... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5292959 Sun, 02 Oct 2011 05:47:51 +0100 5292959 http://www.medworm.com/index.php?rid=5233512&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F050325n312040070%2F Abstract  Vascular remodeling plays a pivotal role in a variety of pathophysiological conditions where hypoxia and inflammation are prominent features. Intravascular ATP, ADP and adenosine are known as important regulators of vascular tone, permeability and homeostasis, however contribution of purinergic signalling to endothelial cell growth and angiogenesis remains poorly understood. By using vasa vasorum endothelial cells (VVEC) isolated from pulmonary artery adventitia of control and chronically hypoxic neonatal calves, these studies were aimed to evaluate the effect of hypoxia on biochemical and functional properties of microvascular endothelial network at the sites of angiogenesis. In comparison with normoxic controls, VVEC from hypoxic animals are characterized by (1)... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5233512 Fri, 16 Sep 2011 05:44:55 +0100 5233512 http://www.medworm.com/index.php?rid=5217700&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl712884q48793474%2F Conclusions  These findings demonstrate that both CE-MRI and CE-US allow monitoring of early changes in vascular function following VDA therapy. The results also demonstrate, for the first time, potent vascular disruptive and antitumor activity of DMXAA against patient tumor-derived head and neck carcinoma xenografts. Content Type Journal ArticleCategory Original PaperPages 1-11DOI 10.1007/s10456-011-9233-1Authors Mukund Seshadri, Department of Pharmacology and Therapeutics, Preclinical Imaging Laboratory, Roswell Park Cancer Institute, Buffalo, NY, USANuno T. Sacadura, VisualSonics Inc., Toronto, ON, CanadaTonya Coulthard, VisualSonics Inc., Toronto, ON, Canada Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5217700 Thu, 08 Sep 2011 05:57:58 +0100 5217700 http://www.medworm.com/index.php?rid=5155693&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fgj01736433737378%2F Abstract  Angiogenesis is a process that is integral to the pathogenesis of high-grade gliomas. Bevacizumab, a humanized monoclonal antibody against vascular endothelial growth factor has emerged as an important therapeutic agent. Data from clinical trials in both recurrent and newly-diagnosed gliomas have shown improved radiological responses and quality of life with acceptable morbidity. However, an improvement in overall survival has not yet been seen and there are concerns on possible change in the pattern of relapse following therapy. Several unanswered questions remain including the dose, timing and sequencing that warrant further research. Content Type Journal ArticleCategory Review PaperPages 1-8DOI 10.1007/s10456-011-9232-2Authors Saroj Kunnakkat, Department ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5155693 Sat, 20 Aug 2011 15:50:57 +0100 5155693 http://www.medworm.com/index.php?rid=5129947&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx3642085365r6568%2F Abstract  Tumor hypoxia is a common determinant of resistance to cytotoxic therapies and metastatic behavior. In rectal cancer patients receiving preoperative chemoradiotherapy, tyrosine kinase activities in tumors with poor and good treatment responses were found to differ. Given that tyrosine kinase signaling mediates hypoxic tissue adaptation, the present study examined whether tumor kinase activity might also correlate with systemic dissemination of rectal cancer. Immunomagnetic selection of disseminated tumor cells (DTC) from bone marrow aspirates was undertaken in 55 patients with locally advanced rectal cancer. Using peptide arrays with 144 tyrosine kinase substrates, phosphopeptide signatures were generated from patients’ baseline tumor biopsies, to study associat... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5129947 Thu, 11 Aug 2011 06:56:11 +0100 5129947 http://www.medworm.com/index.php?rid=5129946&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F700r508826324433%2F Abstract  Prostate cancer (PCa) is the second leading cause of cancer-associated death in men. Once a tumor is established it may attain further characteristics via mutations or hypoxia, which stimulate new blood vessels. Angiogenesis is a hallmark in the pathogenesis of cancer and inflammatory diseases that may predispose to cancer. Heme oxygenase-1 (HO-1) counteracts oxidative and inflammatory damage and was previously reported to play a key role in prostate carcinogenesis. To gain insight into the anti-tumoral properties of HO-1, we investigated its capability to modulate PCa associated-angiogenesis. In the present study, we identified in PC3 cells a set of inflammatory and pro-angiogenic genes down-regulated in response to HO-1 overexpression, in particular VEGFA, VEGFC... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5129946 Thu, 11 Aug 2011 06:56:11 +0100 5129946 http://www.medworm.com/index.php?rid=5076404&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb887716mj14m1114%2F Abstract  Dysregulation of angiogenesis is a common feature of many disease processes. Vascular remodeling is believed to depend on the participation of endothelial progenitor cells, but the identification of endothelial progenitors in postnatal neovascularization remains elusive. Current understanding posits a role for circulating pro-angiogenic hematopoietic cells that interact with local endothelial cells to establish an environment that favors angiogenesis in physiologic and pathophysiologic responses. In the lung, increased and dysregulated angiogenesis is a hallmark of diseases of the bronchial and pulmonary circulations, manifested by asthma and pulmonary arterial hypertension (PAH), respectively. In asthma, THelper-2 immune cells produce angiogenic factors that mobil... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5076404 Wed, 27 Jul 2011 15:48:35 +0100 5076404 http://www.medworm.com/index.php?rid=5076405&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhj472324341q3w17%2F Abstract  Angiogenesis, the sprouting of new blood vessels from the pre-existing vasculature, is a well established target in anti-cancer therapy. It is thought that the Rho GTPase Rac1 is required during vascular endothelial growth factor (VEGF)-mediated angiogenesis. In the present study, we have used a clinically relevant RNA interference approach to silence Rac1 expression. Human umbilical vein endothelial cells were transiently transfected with non-specific control siRNA (siNS) or Rac1 siRNA (siRac1) using electroporation or Lipofectamine 2000. Functional assays with transfected endothelial cells were performed to determine the effect of Rac1 knockdown on angiogenesis in vitro. Silencing of Rac1 inhibited VEGF-mediated tube formation, cell migration, invasion and proli... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5076405 Tue, 26 Jul 2011 05:48:37 +0100 5076405 http://www.medworm.com/index.php?rid=5061506&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq3k2057340042226%2F Abstract  IL-17-induced joint inflammation is associated with increased angiogenesis. However, the mechanism by which IL-17 mediates angiogenesis is undefined. Therefore, the pathologic role of CXCL1 and CXCL5 was investigated in arthritis mediated by local expression of IL-17, employing a neutralizing antibody to each chemokine. Next, endothelial chemotaxis was utilized to examine whether endothelial migration was differentially mediated by CXCL1 and CXCL5. Our results demonstrate that IL-17-mediated disease activity was not affected by anti-CXCL1 treatment alone. In contrast, mice receiving anti-CXCL5 demonstrated significantly reduced clinical signs of arthritis, compared to the mice treated with IgG control. Consistently, while inflammation, synovial lining thickness, b... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5061506 Thu, 21 Jul 2011 18:06:41 +0100 5061506 http://www.medworm.com/index.php?rid=5061507&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F90x6hu16618222u1%2F Content Type Journal ArticlePages 1-2DOI 10.1007/s10456-011-9225-1Authors Kalimuthu Kalishwaralal, Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, Krishnankoil, Tamilnadu 626 190, IndiaSardarpasha Sheikpranbabu, Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, Krishnankoil, Tamilnadu 626 190, IndiaSelvaraj BarathManiKanth, Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, Krishnankoil, Tamilnadu 626 190, IndiaRavinarayanan Haribalaganesh, Department of Biotec... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5061507 Tue, 19 Jul 2011 23:38:59 +0100 5061507 http://www.medworm.com/index.php?rid=5057259&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F17217t45p815w111%2F Content Type Journal ArticlePages 1-2DOI 10.1007/s10456-011-9224-2Authors Banumathi Elayappan, Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, Krishnankoil, Madurai, Tamil Nadu 626190, IndiaHaribalaganesh Ravinarayannan, Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, Krishnankoil, Madurai, Tamil Nadu 626190, IndiaSheik Pran Babu Sardar Pasha, Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University (Kalasalingam Academy of Research and Education), Anand Nagar, Krishnankoil, Madurai, Tamil Nadu 626190, IndiaKyung-jin Lee, Depa... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5057259 Mon, 18 Jul 2011 15:53:27 +0100 5057259 http://www.medworm.com/index.php?rid=5035421&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F483581834u514r8v%2F In conclusion, our data suggests that mini-PEGylated allD is more beneficial in the treatment of RA than unmodified anti-flt-1 peptides, since it has increased stability and the possibility of oral delivery, and could be applied to treat angiogenesis-dependent human diseases, including RA. Content Type Journal ArticlePages 1-12DOI 10.1007/s10456-011-9226-0Authors Jin-Sun Kong, Research Institute of Immunobiology, Catholic Research Institute of Medical Science, Seoul, KoreaSeung-Ah Yoo, Research Institute of Immunobiology, Catholic Research Institute of Medical Science, Seoul, KoreaJong-Hoon Kang, Postech-Catholic Biomedical Engineering Institute, Catholic University of Korea, Seoul, KoreaWooree Ko, Department of Chemical Engineering, Pohang University of Science and Technology, Po... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5035421 Wed, 13 Jul 2011 06:17:04 +0100 5035421 http://www.medworm.com/index.php?rid=5026856&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Frp301667287x1686%2F In this study, we introduce a technique for repeated, microscopic observation of single regressing capillaries in vivo in inflamed murine corneas. Natural capillary regression was initiated by removal of inflammatory stimulus during an active pro-angiogenic phase, while the additional impact of anti-angiogenic treatment with triamcinolone or bevazicumab was investigated. Capillaries regressed naturally within 1 week and treatments did not further enhance the natural regression. Morphologically, early-phase regression was characterized by significant lumen narrowing and a significant reduction in CD11b+ myeloid cell infiltration of the extracellular matrix. By 1 week, vascular remodeling occurred concomitant with CD11b+CD68+KiM2R+ mature macrophage localization on capillary ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=5026856 Sun, 10 Jul 2011 05:50:54 +0100 5026856 http://www.medworm.com/index.php?rid=4997157&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F842v6618gp7l182m%2F Abstract  As umbilical cord blood (UCB) is a rich source of endothelial colony-forming cells (ECFC), our aim was twofold: (1) to examine potential obstetric selection criteria for achieving the highest ECFC yields from UCB units, and (2) to determine whether transient storage temperatures of fresh UCB and cryopreservation of UCB units affected ECFC yield and function. ECFC quality was assessed before and after cryopreservation by their clonogenic proliferative potential. Of the 20 factors examined, placental weight was the only statistically significant obstetric factor that predicted ECFC frequency in UCB. Studies on the effects of storage revealed that transient storage of fresh UCB at 4°C reduced ECFC yield compared with storage at 22°C, while cryopreservation of UCB M... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4997157 Thu, 30 Jun 2011 17:49:19 +0100 4997157 http://www.medworm.com/index.php?rid=4974679&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F28t6873342k01885%2F Abstract  Vascular angiogenesis is regulated by a number of cytokines of which vascular endothelial growth factor (VEGF)-A/and its receptor vascular endothelial growth factor receptor 2 (VEGFR2) play an indisputable role. Similarly lymphangiogenesis is regulated by VEGF-C and its receptor VEGFR3. Currently for treating vasculogenesis diseases such as proliferative retinopathies and cancer, a number of anti-VEGF-A therapies are approved for clinical use. Although clinical efficacies achieved are remarkable, they are found to be transitory in nature, followed by restoration of anti-VEGF therapy resistant angiogenesis. Recently the regulatory role of VEGF-C in initiating and potentiating neo-angiogenesis has been uncovered. Although the interactive nature of VEGF-A and C is kn... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4974679 Thu, 23 Jun 2011 06:00:35 +0100 4974679 http://www.medworm.com/index.php?rid=4932314&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa13111604247j67k%2F Abstract  Amino acids 50–77 (p28) of azurin, a 128 aa cupredoxin isolated from Pseudomonas aeruginosa, is essentially responsible for azurin’s preferential penetration of cancer cells. We now report that p28 also preferentially penetrates human umbilical vein endothelial cells (HUVEC), co-localized with caveolin-1 and VEGFR-2, and inhibits VEGF- and bFGF-induced migration, capillary tube formation and neoangiogenesis in multiple xenograft models. The antiangiogenic effect of p28 in HUVEC is associated with a dose-related non-competitive inhibition of VEGFR-2 kinase activity. However, unlike other antiangiogenic agents that inhibit the VEGFR-2 kinase, p28 decreased the downstream phosphorylation of FAK and Akt that normally precedes cellular repositioning of the cytoskele... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4932314 Sat, 11 Jun 2011 06:31:47 +0100 4932314 http://www.medworm.com/index.php?rid=4901305&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd072212290k51534%2F Abstract  Von Hippel-Lindau (VHL) disease results from the inactivation of the VHL gene and is characterized by highly vascular tumors. A consequence of VHL loss is the stabilization of hypoxia-inducible factor (HIF) alpha subunits and increased expression of HIF target genes, which include pro-angiogenic growth factors such as vascular endothelial growth factor (VEGF). In mice, homozygous deletion of VHL is embryonic lethal due to vascular abnormalities in the placenta; and, VHL+/− mice develop proliferative vascular lesions in several major organs, most prominently the liver. Loss of ELL-associated factor (EAF2) in murine models has also been shown to induce increased vascular density in the liver as well as the prostate. Previously, EAF2 was determined to be a binding p... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4901305 Fri, 03 Jun 2011 05:55:25 +0100 4901305 http://www.medworm.com/index.php?rid=4901306&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh046672622247l25%2F Abstract  Transmembrane-4-L-six-family-1 (TM4SF1) is a tetraspanin-like membrane protein that is highly and selectively expressed by cultured endothelial cells (EC) and, in vivo, by EC lining angiogenic tumor blood vessels. TM4SF1 is necessary for the formation of unusually long (up to a 50 μm), thin (~100–300 nm wide), F-actin-poor EC cell projections that we term ‘nanopodia’. Immunostaining of nanopodia at both the light and electron microsopic levels localized TM4SF1 in a regularly spaced, banded pattern, forming TM4FS1-enriched domains. Live cell imaging of GFP-transduced HUVEC demonstrated that EC project nanopodia as they migrate and interact with neighboring cells. When TM4SF1 mRNA levels in EC were increased from the normal ~90 mRNA copies/cell to ~... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4901306 Mon, 30 May 2011 17:13:42 +0100 4901306 http://www.medworm.com/index.php?rid=4852319&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb1h3878066731hrq%2F Content Type Journal ArticlePages 1-2DOI 10.1007/s10456-011-9216-2Authors Joyce Bischoff, Boston, MA, USAArjan W. Griffioen, Angiogenesis Laboratory, Department of Medical Oncology, VUMC – Cancer Center Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4852319 Wed, 18 May 2011 18:16:54 +0100 4852319 http://www.medworm.com/index.php?rid=4811969&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F222379238126635j%2F Conclusion  It is suggested that assessment of taurine level in sera of patients with high risk for breast cancer are of great value in the early diagnosis of malignant changes in the breast. Content Type Journal ArticlePages 1-10DOI 10.1007/s10456-011-9215-3Authors I. M. El Agouza, Faculty of Science, Cairo University, Cairo, EgyptS. S. Eissa, National Cancer Institute, Cairo University, Cairo, EgyptM. M. El Houseini, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, EgyptDalia E. El-Nashar, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, EgyptO. M. Abd El Hameed, BMES Department, High Institute of Engineering, Shorouk-Academy, Cairo, Egypt Journal AngiogenesisOnline ISSN 1573-7209Prin... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4811969 Sat, 07 May 2011 15:45:02 +0100 4811969 http://www.medworm.com/index.php?rid=4794216&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp740132w01x24035%2F Abstract  Phosphatidylinositol 4-phosphate 5-kinase (PI(4)P5K) is a type I lipid kinase that generates the lipid second messenger phospholipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and functions downstream of RhoA in actin organization. It is known to play an essential role in neurite remodeling, yielding a phenotype identical to that seen in cells treated with Semaphorin 4D (Sema4D), a protein that regulates proliferation, adhesion and migration in many different cell types. Plexin-B1, the receptor for Sema4D, activates RhoA in order to generate a pro-angiogenic signal in endothelial cells. Therefore, we looked in human umbilical vein endothelial cells (HUVEC) to determine if Plexin-B1 exerted control over the cytoskeleton by regulation of PI(4)P5K activity. He... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4794216 Tue, 03 May 2011 05:57:16 +0100 4794216 http://www.medworm.com/index.php?rid=4770689&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl7775155015m7052%2F Abstract  Cancer cells produce galectin-1 as a tumor promoting protein. Thiodigalactoside (TDG) as a non-metabolised small drug, is shown to suppress tumor growth by inhibiting multiple cancer enhancing activities of galectin-1, including immune cell dysregulation, angiogenesis and protection against oxidative stress. Thus, using B16F10 melanoma and 4T1 orthotopic breast cancer models, intratumoral injection of TDG significantly raised the levels of tumor-infiltrating CD8+ lymphocytes and reduced CD31+ endothelial cell content, reducing tumor growth. TDG treatment of tumors in Balb/c nude mice (defective in T cell immunity) reduced angiogenesis and slowed tumor growth by a third less than in immunocompetent mice. Knocking down galectin-1 expression (G1KD) in both cancer cell... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4770689 Tue, 26 Apr 2011 15:46:54 +0100 4770689 http://www.medworm.com/index.php?rid=4744640&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5467181q75q88014%2F Abstract  Endothelial progenitor cells (EPCs) play a critical role in the repair of damaged blood vessels and/or in the growth of new ones in ischemic tissues. Elevated levels of oxygen radicals, which accumulate in the ischemic tissue, could compromise the angiogenic potential of EPCs. To determine if oxidative stress alters the angiogenic response of EPCs and to identify possible cellular targets that protect EPCs from the damaging effects of oxidative stress, we have investigated vascular development in embryonic bodies (EBs) under hyperoxic conditions. Murine EBs at differentiaton day 2 were cultured for 3 days under normoxic (21% O2) or hyperoxic (60% O2) conditions. Hyperoxic EBs showed a moderate reduction in Pecam-1, Vegfr-2, eNOS and Tie2 mRNA levels compared t... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4744640 Sat, 16 Apr 2011 15:57:35 +0100 4744640 http://www.medworm.com/index.php?rid=4722975&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F88340n2h2702x68m%2F This study provides first information on how to modify roscovitine to develop Cdk inhibitors with increased antiangiogenic activity and suggests the application of existing and the development of new Cdk inhibitors to inhibit both, cancer cell proliferation and angiogenesis. Content Type Journal ArticlePages 1-11DOI 10.1007/s10456-011-9212-6Authors Johanna Liebl, Department of Pharmacy, Center for Drug Research, Ludwig Maximilians University, Butenandtstr. 5-13, 81377 Munich, GermanyVladimir Krystof, Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, 78371 Olomouc, Czech RepublicGyörgy Vereb, Medical and Health Science Centre, Department of Biophysics and Cell Biology, University of Debrecen, 4032 Debrecen, HungaryL... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4722975 Tue, 12 Apr 2011 21:37:16 +0100 4722975 http://www.medworm.com/index.php?rid=4722976&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn78213x4g7334824%2F Abstract  Neuroblastoma is the most common pediatric abdominal tumor and principally a p53 wild-type, highly vascular, aggressive tumor, with limited response to anti-VEGF therapies alone. MDM2 is a key inhibitor of p53 and a positive activator of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) activity with an important role in neuroblastoma pathogenesis. We hypothesized that concurrent inhibition of both MDM2 and VEGF signaling would have cooperative anti-tumor effects, potentiating anti-angiogenic strategies for neuroblastoma and other p53 wild-type tumors. We orthotopically implanted SH-SY5Y neuroblastoma cells into nude mice (n = 40) and treated as follows: control, bevacizumab, Nutlin-3a, combination of bevacizumab plus Nu... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4722976 Tue, 12 Apr 2011 06:54:49 +0100 4722976 http://www.medworm.com/index.php?rid=4669564&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft68151n6145q23w3%2F In this report, the ability of PEG-SN38 to down-regulate HIF-1α and its downstream targets, in a more potent, sustained manner compared with CPT-11 was examined. To do so, U251 glioma xenografts that stably expressed a hypoxia response element-dependent luciferase reporter gene were implanted in mice. After treatment it was found that EZN-2208 induced potent, sustained HIF-1α down-regulation (37% at 48 h and 83% at 120 h) in the tumors, whereas CPT-11 caused only minor, transient HIF-1α down-regulation. In addition, EZN-2208 down-regulated mRNA levels of HIF-1α targeted genes (MMP2, VEGF1, Glut1, Glut3 and TGFβ1). Further, western blot analyses of xenograft tumors demonstrated that EZN-2208 had significantly more effect than CPT-11 in down-regulating HIF-1α, VEGF, Glu... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4669564 Thu, 31 Mar 2011 07:21:27 +0100 4669564 http://www.medworm.com/index.php?rid=4641703&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr32r858648133183%2F Conclusions/significance  PMN derived MMP-9 acts as a potent, direct and VEGF independent angiogenic factor in the context of PDAC. MMP-9 inhibition is as effective as VEGF inhibition. Targeting MMP-9 in addition to VEGF is therefore likely to be important for successful anti-angiogenic treatment in pancreatic cancer. Content Type Journal ArticlePages 1-9DOI 10.1007/s10456-011-9207-3Authors Dirk Bausch, Department of General and Visceral Surgery, University of Freiburg, Hugstetter Str. 55, 79106 Freiburg, GermanyThomas Pausch, Klinik für Allgemein, Viszeral- und Transplantationschirurgie, Universität Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, GermanyTobias Krauss, Radiologische Universitätsklinik Freiburg i. Br., Hugstetter Str. 55, 79106 Freiburg i. B... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4641703 Fri, 25 Mar 2011 18:51:57 +0100 4641703 http://www.medworm.com/index.php?rid=4635892&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7230518910204753%2F Abstract  The paradigm of a therapy aimed at inhibiting the formation of blood vessels, which would consequentially deprive cells and tissues of oxygen and nutrients, was born from the concept pioneered by the late Judah Folkman that blood vessel formation is central to the progression and maintenance of diseases which involve cellular metabolism and tissue expansion, and cancer in particular. The prototype targeted angiogenesis inhibitor anti-vascular endothelial growth factor (VEGF) antibody bevacizumab was approved in 2004 for colorectal cancer, and has since been approved for other cancers. Rheumatoid arthritis (RA) is a chronic inflammatory disease, during which inflamed tissue invades and destroys cartilage and bone. The tissue expansion, invasion, expression of cytok... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4635892 Tue, 22 Mar 2011 18:09:22 +0100 4635892 http://www.medworm.com/index.php?rid=4610209&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ftu0m67h86237m711%2F Abstract  We have recently shown that aldose reductase (AR, EC 1.1.1.21) a nicotinamide adenine dinucleotide phosphate-dependent aldo–keto reductase, known to be involved in oxidative stress-signaling, prevents human colon cancer cell growth in culture as well as in nude mice xenografts. Inhibition of AR also prevents azoxymethane-induced aberrant crypt foci formation in mice. In order to understand the chemopreventive mechanism(s) of AR inhibition in colon cancer, we have investigated the role of AR in the mediation of angiogenic signals in vitro and in vivo models. Our results show that inhibition of AR significantly prevented the VEGF- and FGF -induced proliferation and expression of proliferative marker Ki67 in the human umbilical vein endothelial cells (HUVEC). Furth... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4610209 Wed, 16 Mar 2011 12:02:14 +0100 4610209 http://www.medworm.com/index.php?rid=4494445&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj158172p170782w2%2F Abstract  Tissues require an adequate supply of oxygen in order to maintain normal cell function. Low oxygen tension (hypoxia) is characteristic of a number of conditions, including cancer, atherosclerosis, rheumatoid arthritis, critical limb ischaemia, peripheral vascular disease, and ischaemic heart disease. Tissue hypoxia is found in tumours, atherosclerotic plaque, and ischaemic myocardium. There is a growing interest in methods to detect and assess hypoxia, given that hypoxia is important in the progression of these diseases. Hypoxia can be assessed at the level of the whole organ, tissue, or cell, using both invasive and non-invasive methods, and by a range of immunohistochemical, biochemical, or imaging techniques. This review describes and critiques current methods ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4494445 Wed, 16 Feb 2011 06:55:14 +0100 4494445 http://www.medworm.com/index.php?rid=4494444&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F34824m1636842330%2F Abstract   Radix Angelica sinensis is a Chinese medicinal herb that has been used extensively in the East for the treatment of cardiovascular diseases (CVDs). Angiogenesis plays an important role in the pathogenesis of CVDs. We hypothesized that Radix A. sinensis may contain angiogenesis modulators. In the current study, we investigated the effects of a volatile oil of Radix A. sinensis (VOAS) and n-butylidenephthalide (BP), one of the bioactive components in VOAS, on angiogenesis in vitro and in vivo. The results suggested that VOAS exerted anti-angiogenic effects by inhibiting human umbilical vein endothelial cell proliferation, migration and capillary-like tube formation on Matrigel. BP was also shown to be anti-angiogenic and its mechanisms were through inhibition of cel... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4494444 Wed, 16 Feb 2011 06:55:14 +0100 4494444 http://www.medworm.com/index.php?rid=4488798&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa287656531w32112%2F Content Type Journal ArticlePages 1-47DOI 10.1007/s10456-011-9203-7 Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4488798 Mon, 14 Feb 2011 07:10:22 +0100 4488798 http://www.medworm.com/index.php?rid=4488799&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F0h08l81144007q31%2F Abstract  Hypoxia results in the apoptotic death of myocytes, neurons, and epithelial cells, through the actions of Bcl-2 and Nineteen kilodalton Interacting Protein-3 (BNIP3). On the contrary, endothelial cells are especially adept at surviving conditions of oxygen deprivation via up-regulation of vascular endothelial growth factor (VEGF) the most potent endothelial survival factor. Both VEGF and BNIP3 expression are transcriptionally regulated by hypoxia inducible factor and may antagonize each other’s affects in endothelial cells (ECs). Since factors that promote and inhibit apoptosis may be expressed at the same time in endothelial cells under hypoxic conditions, we decided to investigate whether VEGF and BNIP3 have opposing actions in endothelial cells. Human microva... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4488799 Mon, 14 Feb 2011 06:50:04 +0100 4488799 http://www.medworm.com/index.php?rid=4404935&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F121432n5l72m1183%2F In this report we demonstrate GSK-3β’s role as a signaling node in two mouse strains (C57BL/6, which have been found to respond to and recover from a hypoxic insult from P3 to P11 poorly and CD-1, which have been found to respond to and recover from a hypoxic insult from P3 to P11 well both in vivo and in vitro) which mimic the wide range of responsiveness to hypoxic insult observed in the very low birth weight premature infant population. Differences in levels of neural stem cell and microvascular endothelial cell GSK-3β activation, β-catenin serine phosphorylation, HIF-1α and 2α, BDNF, SDF-1 and VEGF, β-III-tubulin and cleaved notch-1 expression in C57BL/6 and CD-1 subventricular zone tissues, and cultured NSC and BEC were noted. Specifically, CD1 pups, SVZ tissues and isol... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4404935 Fri, 21 Jan 2011 15:24:35 +0100 4404935 http://www.medworm.com/index.php?rid=4355305&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh84q173885123077%2F Abstract  Recently, we isolated and characterized resident endothelial progenitor cells from the lungs of adult mice. These cells have a high proliferation potential, are not transformed and can differentiate into blood- and lymph-vascular endothelial cells under in vitro and in vivo conditions. Here we studied the secretome of these cells by nanoflow liquid chromatographic mass spectrometry (LC–MS). For analysis, 3-day conditioned serum-free media were used. We found 133 proteins belonging to the categories of membrane-bound or secreted proteins. Thereby, several of the membrane-bound proteins also existed as released variants. Thirty-five proteins from this group are well known as endothelial cell- or angiogenesis-related proteins. The MS analysis of the secretome was s... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4355305 Fri, 14 Jan 2011 14:17:49 +0100 4355305 http://www.medworm.com/index.php?rid=4342293&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh81375l6112j468h%2F In this study, we determined the anti-angiogenic activity of a single dose of liposomal prednisolone phosphate (PLP-L), by monitoring tumor vascular function and viability over a period of one week. C57BL/6 mice were inoculated subcutaneously with B16F10 melanoma cells. Six animals were PLP-L-treated and six served as control. Tumor tissue and vascular function were probed using MRI before and at three timepoints after treatment. DCE-MRI was used to determine Ktrans, ve, time-to-peak, initial slope and the fraction of non-enhancing pixels, complemented with immunohistochemistry. The apparent diffusion coefficient (ADC), T2 and tumor size were assessed with MRI as well. PLP-L treatment resulted in smaller tumors and caused a significant drop in Ktrans 48 h post-treatment, which wa... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4342293 Tue, 11 Jan 2011 18:03:20 +0100 4342293 http://www.medworm.com/index.php?rid=4342294&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fjxj74616k8q27k85%2F Conclusion  We demonstrated that tumours of patients with platinum resistant EOC exhibit higher levels of VEGF expression compared to the platinum sensitive group. VEGF in EOC, may be of clinical and therapeutic relevance and suggests a role for first line anti-angiogenic therapy. Content Type Journal ArticlePages 1-7DOI 10.1007/s10456-010-9199-4Authors G. K. Siddiqui, Department of Obstetrivs and Gynaecology, Royal Free and University College Medical School, London, UKA. B. Maclean, Department of Obstetrivs and Gynaecology, Royal Free and University College Medical School, London, UKK. Elmasry, Division of Gynaecological Oncology, Tawam Hospital in affiliation with John Hopkins, Al Ain, United Arab EmiratesA. Wong te Fong, Department of Obstetrivs and Gynaecology, R... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4342294 Mon, 10 Jan 2011 16:15:16 +0100 4342294 http://www.medworm.com/index.php?rid=4301973&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr0u4v3463670664h%2F We report here that treatment of tumor bearing mice with recombinant VEGI leads to a significantly decreased population of BM-derived EPC in the tumors. We transplanted whole bone marrow from green fluorescent protein (GFP) transgenic mice into C57BL/6 recipient mice, which were then inoculated with Lewis lung carcinoma (LLC) cells. Intraperitoneal injection of recombinant VEGI led to significant inhibition of tumor growth and decrease of vasculature density compared to vehicle-treated mice. Tumor implantation yielded a decrease of BM-derived EPC in the peripheral blood, while VEGI-treatment resulted in an initial delay of such decrease. Analysis of the whole bone marrow showed a decrease of Lin−-c-Kit+-Sca-1+ hematopoietic stem cell (HSC) population in tumor bearing mice; however,... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4301973 Tue, 28 Dec 2010 15:34:51 +0100 4301973 http://www.medworm.com/index.php?rid=4301974&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F1352800165087174%2F In conclusion, while clinical awareness that antiangiogenic treatment can cause GI perforations is critical for current medical practice, it is also very important to get more insight in its underlying mechanisms so that this life-threatening complication may be prevented in the near future. Content Type Journal ArticleDOI 10.1007/s10456-010-9197-6Authors Maudy Walraven, Department of Medical Oncology, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The NetherlandsPetronella O. Witteveen, Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The NetherlandsMartijn P. J. Lolkema, Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The NetherlandsR. van Hillegersberg, Department of Surgery, University Medical Center ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4301974 Tue, 28 Dec 2010 15:34:49 +0100 4301974 http://www.medworm.com/index.php?rid=4288347&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx016v64281886064%2F Abstract  An MHC-mismatch bone marrow (BM) transplant Ewing’s sarcoma mouse model was used to investigate whether BM cells participate in the vessel formation that support Ewing’s sarcoma lung metastasis. BM cells from H-2Kb/d donor mice were transplanted into sublethally irradiated H-2Kd recipient mice. Donor BM cells were identified using the H-2Kb marker. Engraftment was confirmed by identifying the H-2Kb IL-1β-type specific polymorphism. After engraftment highly lung metastatic TC71-PM4 cells were injected intravenously. Mice were sacrificed 10 weeks after tumor cell injection. Hematoxylin-and-eosin staining was performed to identify lung metastatic foci. These tumors were then evaluated using immunohistochemical analysis. H-2Kb-positive cells were found in lung ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4288347 Thu, 23 Dec 2010 17:49:42 +0100 4288347 http://www.medworm.com/index.php?rid=4196284&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx545161327v62076%2F Abstract  Angiogenesis is one of the major processes controlling growth and metastasis of tumors. Angiogenesis inhibitors have been targeted for the treatment of various cancers for more than 2 decades. We have developed a novel class of steroidal compounds aimed at blocking the angiogenic process in cancerous tissues. Our lead compound, SR16388, is a potent antiangiogenic agent with binding affinity to estrogen receptor-α (ER-α) and -β (ER-β) at the nanomolar range. This compound inhibited the proliferation of human microvascular endothelial cells (HMVEC) and various types of human cancer cells in vitro. SR16388 inhibited embryonic angiogenesis as measured in the chick chorioallantoic membrane (CAM) assay. The blood vessel density in the CAM was greatly reduced after t... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4196284 Sat, 20 Nov 2010 17:51:01 +0100 4196284 http://www.medworm.com/index.php?rid=4196283&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7p62p154p378474u%2F Abstract  Using a fibrin-based angiogenesis model, we have established that there is no canonical mechanism used by endothelial cells (ECs) to degrade the surrounding extracellular matrix (ECM), but rather the set of proteases used is dependent on the mural cells providing the angiogenic cues. Mesenchymal stem cells (MSCs) originating from different tissues, which are thought to be phenotypically similar, promote angiogenesis through distinct mechanisms. Specifically, adipose-derived stem cells (ASCs) promote utilization of the plasminogen activator-plasmin axis by ECs as the primary means of vessel invasion and elongation in fibrin. Matrix metalloproteinases (MMPs) serve a purpose in regulating capillary diameter and possibly in stabilizing the nascent vessels. These prote... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4196283 Sat, 20 Nov 2010 17:51:01 +0100 4196283 http://www.medworm.com/index.php?rid=4149835&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F36070m6m480t0533%2F Abstract  The purpose of this study was to investigate the effect of gold nanoparticles on the signaling cascade related to angiogenesis and vascular permeability induced by Vascular Endothelial Growth Factor (VEGF) in Bovine retinal endothelial cells (BRECs). The effect of VEGF and gold nanoparticles on cell viability, migration and tubule formation was assessed. PP2 (Src Tyrosine Kinase inhibitor) was used as the positive control and the inhibitor assay was performed to compare the effect of AuNPs on VEGF induced angiogenesis. The transient transfection assay was performed to study the VEGFR2/Src activity during experimental conditions and was confirmed using western blot analysis. Treatment of BRECs with VEGF significantly increased the cell proliferation, migration and ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4149835 Mon, 08 Nov 2010 19:18:42 +0100 4149835 http://www.medworm.com/index.php?rid=4142466&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd64645v722626531%2F Conclusions  ECFC may partly mediate the clinical benefits of prostanoids in pulmonary arterial hypertension. Content Type Journal ArticleDOI 10.1007/s10456-010-9192-yAuthors David M. Smadja, Université Paris Descartes, Paris, FranceLaetitia Mauge, Université Paris Descartes, Paris, FrancePascale Gaussem, Université Paris Descartes, Paris, FranceClément d’Audigier, Université Paris Descartes, Paris, FranceDominique Israel-Biet, Université Paris Descartes, Paris, FranceDavid S. Celermajer, Sydney Medical School, University of Sydney, Sydney, NSW 2006, AustraliaDamien Bonnet, Université Paris Descartes, Paris, FranceMarilyne Lévy, Université Paris Descartes, Paris, France Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4142466 Wed, 03 Nov 2010 17:03:42 +0100 4142466 http://www.medworm.com/index.php?rid=4096024&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9032442741223864%2F Abstract  Vascular Endothelial Growth Factor Receptor (VEGFR) mediated signalling drives angiogenesis. This is predominantly attributed to the activity of VEGFR-2 following binding of VEGF-A. Whether other members of the VEGFR and ligand families such as VEGFR-1 and its ligand Placental Growth Factor (PlGF) can also contribute to developmental and pathological angiogenesis is less clear. We explored the function of PlGF in VEGF-A dependent angiogenesis using an in vitro co-culture assay in which endothelial cells are cultured on a fibroblast feeder layer. In the presence of 2% FS MCDB media (containing limited growth factors) in vitro endothelial tube formation is driven by endogenous angiogenic stimuli which are produced by the fibroblast and endothelial cells. Under these... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4096024 Sat, 16 Oct 2010 09:04:52 +0100 4096024 http://www.medworm.com/index.php?rid=4044365&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn1162128202m0756%2F Abstract  Resveratrol, a naturally occurring polyphenol, has been reported to be an anti-tumor and chemopreventive agent. Recent data show that it may also exert anti-angiogenic effects. We hypothesized that the anti-angiogenic activity of resveratrol may be caused by modulation of tumor cell release of thrombospondin-1 (TSP1) and vascular endothelial growth factor (VEGF) into the extracellular matrix, leading to vascular endothelial cell (VEC) apoptosis. We therefore evaluated the effects of resveratrol on melanoma cell lines co-cultured with vascular endothelial cells in monolayer and in three dimensional spheroids. We found that resveratrol stimulated isolated VEC proliferation, while it caused growth inhibition of VECs grown with melanoma cells in three-dimensional co-c... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4044365 Wed, 06 Oct 2010 16:45:19 +0100 4044365 http://www.medworm.com/index.php?rid=4002662&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F34544g6260r25038%2F Conclusions  We demonstrate expression of NF-κB target genes in proliferating IH. In addition, we show that the expression of several pro-angiogenic factors in HemSCs, and in particular VEGF–A, is regulated by NF-B activity. Content Type Journal ArticleDOI 10.1007/s10456-010-9189-6Authors Shoshana Greenberger, Vascular Biology Program and Department of Surgery, Children’s Hospital Boston and Harvard Medical School, Boston, MA 02115, USAIrit Adini, Vascular Biology Program and Department of Surgery, Children’s Hospital Boston and Harvard Medical School, Boston, MA 02115, USAElisa Boscolo, Vascular Biology Program and Department of Surgery, Children’s Hospital Boston and Harvard Medical School, Boston, MA 02115, USAJohn B. Mulliken, Department of Plastic S... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=4002662 Fri, 24 Sep 2010 17:07:27 +0100 4002662 http://www.medworm.com/index.php?rid=3981887&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw83h46n6449810q0%2F Abstract  Impaired wound healing is a persistent clinical problem which has been treated with mixed results. Studies aimed at elucidating the mechanism of impaired wound healing have focused on small cohorts of genes which leave an incomplete picture of the wound healing process. We aimed to investigate impaired wound healing via a comprehensive panel of angiogenic/inflammation-related genes and wound closure kinetics with and without the application of extracorporeal shock wave therapy (ESWT), which has been demonstrated to improve wound healing. Full-thickness skin from the dorsal surface of “normal” (BALB/c) and “impaired” (db +/db +) mice was excised, and wound margin tissue was harvested 2, 7, and 10 days post injury. A separate, but identical wound model ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3981887 Fri, 17 Sep 2010 06:47:10 +0100 3981887 http://www.medworm.com/index.php?rid=3981888&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv7k0382272781u15%2F Conclusions  EPLINα over-expression can regulate HECV cell motility, matrix adhesion and tubule formation in vitro and slow in vivo tumour formation, suggesting an anti-angiogenic role for EPLINα. Extracellular signal-regulated kinase (ERK) also appears to be linked to the ability of EPLINα to inhibit tubule formation in vitro. Content Type Journal ArticleDOI 10.1007/s10456-010-9188-7Authors Andrew J. Sanders, Metastasis and Angiogenesis Research Group, Cardiff University School of Medicine, Cardiff, CF14 4XN UKLin Ye, Metastasis and Angiogenesis Research Group, Cardiff University School of Medicine, Cardiff, CF14 4XN UKMalcolm D. Mason, Department of Clinical Oncology, Cardiff University School of Medicine, Cardiff, UKWen G. Jiang, Metastasis and Angiogenesis Res... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3981888 Fri, 17 Sep 2010 06:47:09 +0100 3981888 http://www.medworm.com/index.php?rid=3980188&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6347135760502201%2F Abstract  Photodynamic therapy (PDT) induces damage to the endothelium, which can lead to increased vascular permeability and, under intensive PDT conditions, even to platelet aggregation, vasoconstriction, and blood flow stasis. Eventually, ischemia, hypoxia, and inflammation can occur, resulting in angiogenesis. We studied the sequence of the vascular events after Visudyne®-PDT in the chicken chorioallantoic membrane (CAM) at day 11 of development. Using epi-fluorescence microscopy, we monitored the regrowth of capillaries in the PDT treated area. Immediately after irradiation, the treatment resulted in blood flow arrest. And 24 h post PDT, sprouting of new blood vessels was observed at the edge of the PDT zone. Neovessels looping out from the edge of the PDT zone ga... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3980188 Tue, 14 Sep 2010 18:10:26 +0100 3980188 http://www.medworm.com/index.php?rid=3932633&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh0l577287321g082%2F Abstract  In ischemic retinopathies, unrelieved hypoxia induces the formation of architecturally abnormal, leaky blood vessels that damage retina and ultimately can cause blindness. Because these newly formed blood vessels are functionally defective, they fail to alleviate underlying hypoxia, resulting in more pathological neovascularization and more damage to retina. With an established model of ischemic retinopathy, we investigated inhibition of glycogen synthase kinase-3β (GSK-3β) as a means for improving the architecture and functionality of pathological blood vessels in retina. In vitro, hypoxia increased GSK-3β activity in retinal endothelial cells, reduced β-catenin, and correspondingly impaired integrity of cell/cell junctions. Conversely, GSK-3β inhibitors res... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3932633 Thu, 02 Sep 2010 06:53:55 +0100 3932633 http://www.medworm.com/index.php?rid=3920111&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhw2k177057756700%2F Abstract  EphB4 and its cognitive ligand ephrinB2 play an important role in embryonic vessel development and vascular remodeling. In addition, several reports suggest that this receptor ligand pair is also involved in pathologic vessel formation in adults including tumor angiogenesis. Eph/ephrin signaling is a complex phenomena characterized by receptor forward signaling through the tyrosine kinase of the receptor and ephrin reverse signaling through various protein–protein interaction domains and phosphorylation motifs of the ephrin ligands. Therefore, interfering with EphR/ephrin signaling by the means of targeted gene ablation, soluble receptors, dominant negative mutants or antisense molecules often does not allow to discriminate between inhibition of Eph/ephrin forwa... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3920111 Sat, 28 Aug 2010 17:05:22 +0100 3920111 http://www.medworm.com/index.php?rid=3898852&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn6815m5325l0441t%2F Abstract  The development of a new, less invasive, and more rapidly implemented method of quantifying endothelial cell density in tumors could facilitate experimental and clinical studies of angiogenesis. Therefore, we evaluated the utility of tumor fine needle aspiration (FNA) coupled with flow cytometry for assessment of tumor angiogenesis. Samples were obtained from cutaneous tumors of mice using FNA, then immunostained and assessed by flow cytometry to determine the number of CD31+ endothelial cells. Results of the FNA/flow cytometry technique were compared with quantification of tumor microvessel density using immunohistochemistry. The ability of the FNA/cytometry technique to quantify the effects of anti-angiogenic therapy and to monitor changes in tumor angiogenesis o... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3898852 Mon, 23 Aug 2010 17:58:48 +0100 3898852 http://www.medworm.com/index.php?rid=3866756&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F35017772l68250p0%2F Abstract  Small molecular kinase inhibitors are promising novel drugs. Initially, they were designed for the highest possible specificity. Recently, this concept has been challenged by multikinase inhibitors, which are clinically more potent. This change of paradigm calls for re-examination of already known compounds in different functional contexts. We have compared 6 reported structurally different inhibitors of cyclin-dependent kinases (Cdks) regarding their functional effects on endothelial cells (proliferation, cell cycle, apoptosis, migration, tube formation), as well as their actions on some kinases (AKT, p38, ERK1/2, c-src, GSK3β). Only some of these compounds had anti-angiogenic effects in concentrations up to 10 μM (aminopurvalanol, indirubin-3′-monoxime, ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3866756 Thu, 12 Aug 2010 18:44:16 +0100 3866756 http://www.medworm.com/index.php?rid=3861426&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fch00277v42637773%2F Conclusion  Similar to cancer cells, ECs induced autophagy in response to the pro-apoptotic agent, SUL, and the inhibition of autophagy potentiated the pro-apoptotic effect. These findings open premises for the use of autophagy inhibitors in combination with anti–angiogenic agents. Content Type Journal ArticleDOI 10.1007/s10456-010-9180-2Authors Takeshi Nishikawa, Department of Surgical Oncology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655 JapanNelson H. Tsuno, Department of Surgical Oncology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655 JapanYurai Okaji, Department of Transfusion Medicine, Faculty of Medicine, The University of Tokyo, Tokyo, JapanEiji Sunami, Department of Surgical ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3861426 Sat, 07 Aug 2010 16:49:01 +0100 3861426 http://www.medworm.com/index.php?rid=3749670&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm008rl6161366210%2F Abstract  Solid tumor growth is heavily dependant on angiogenesis. Tumor angiogenesis is the result of a complex interplay between tumor cells, endothelial cells, and other stromal cells. It has been found to be under strict control of a plethora of molecular factors that function as angiogenic up- and down-regulators; nevertheless, the identification of molecular and cellular players and their roles in angiogenesis is still ongoing. The microvasculature resulting from tumor angiogenesis lacks hierarchy and has a high permeability for macromolecules and nanoparticles, which offers significant potential for nanoparticulate tumor imaging and drug delivery platforms. However, improvements in the delivery to poorly vascularized regions and the distribution throughout the tumor ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3749670 Sat, 10 Jul 2010 06:46:12 +0100 3749670 http://www.medworm.com/index.php?rid=3694811&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft453841g6348287j%2F Abstract  Rings of rat or mouse aorta embedded in collagen gels produce angiogenic outgrowths in response to the injury of the dissection procedure. Aortic outgrowths are composed of branching endothelial tubes and surrounding mural cells. Mural cells emerge following endothelial sprouting and gradually increase during the maturation of the neovessels. Treatment of aortic cultures with angiopoietin-1 (Ang-1), an angiogenic factor implicated in vascular maturation and remodeling, stimulates the mural cell recruitment process. Ang-1 induces expression of many cytokines and chemokines including monocyte chemotactic protein-1 (MCP-1). Inhibition of p38 MAP kinase, a signaling molecule required for mural cell recruitment, blocks Ang1-induced MCP-1 expression. Recombinant MCP-1 d... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3694811 Tue, 22 Jun 2010 18:35:11 +0100 3694811 http://www.medworm.com/index.php?rid=3678424&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5483424821303vv2%2F Content Type Journal ArticleCategory Editorial NoteDOI 10.1007/s10456-010-9178-9Authors Willem J. M. Mulder, Mount Sinai School of Medicine Translational and Molecular Imaging Institute One Gustave L. Levy Place Box 1234 New York NY USAArjan W. Griffioen, VU University Medical Center Angiogenesis Laboratory, Department of Medical Oncology, VUmc-Cancer Center Amsterdam Amsterdam The Netherlands Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3678424 Fri, 18 Jun 2010 10:36:42 +0100 3678424 http://www.medworm.com/index.php?rid=3670891&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F0m5216655p322160%2F Abstract  Our group has previously reported that SNPs in the VEGF promoter are strongly associated with efficacy and toxicity to the anti-VEGF antibody bevacizumab in breast cancer. In order to better understand the biologic mechanism for our previously reported biomarkers, we embarked on a comprehensive evaluation of genetic variation in the VEGF promoter coupled with a study of its intrinsic function. We resequenced 48 Caucasians and 48 African-Americans for the VEGF promoter to identify SNPs and elucidate its haplotype structure. We further cloned the haplotypes into reporter constructs and assessed the role of SNPs on promoter function in breast cancer cell lines. SNPs that were identified included twenty previously reported SNPs/insertions/deletions, one novel SNP, and... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3670891 Wed, 16 Jun 2010 07:26:17 +0100 3670891 http://www.medworm.com/index.php?rid=3670890&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8kxw5v0008679517%2F Abstract  Quantum dots exhibit unique optical properties for bioimaging purposes. We have previously developed quantum dots with a paramagnetic and functionalized coating and have shown their potential for molecular imaging purposes. In the current mini-review we summarize the synthesis procedure, the in vitro testing and, importantly, the in vivo application for multimodal molecular imaging of tumor angiogenesis. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10456-010-9177-xAuthors Willem J. M. Mulder, Mount Sinai School of Medicine Translational and Molecular Imaging Institute One Gustave L. Levy Place Box 1234 New York NY 10029 USAGustav J. Strijkers, Eindhoven University of Technology Biomedical NMR, Department of Biomedical Engineering Eindho... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3670890 Wed, 16 Jun 2010 07:26:17 +0100 3670890 http://www.medworm.com/index.php?rid=3670892&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff6222g8745743511%2F Abstract  Stroke is a major cause of mortality and long-term disability worldwide. The initial changes in local perfusion and tissue status underlying loss of brain function are increasingly investigated with noninvasive imaging methods. In addition, there is a growing interest in imaging of processes that contribute to post-stroke recovery. In this review, we discuss the application of magnetic resonance imaging (MRI) to assess the formation of new vessels by angiogenesis, which is hypothesized to participate in brain plasticity and functional recovery after stroke. The excellent soft tissue contrast, high spatial and temporal resolution, and versatility render MRI particularly suitable to monitor the dynamic processes involved in vascular remodeling after stroke. Here we ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3670892 Wed, 16 Jun 2010 07:26:16 +0100 3670892 http://www.medworm.com/index.php?rid=3670893&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc124333480042043%2F Abstract  Angiogenesis, the growth of new blood vessels, plays a critical role in progression of tumor growth and metastasis, making it an attractive target for both cancer imaging and therapy. Several molecular markers, including those that are involved in the angiogenesis signaling pathway and those unique to tumor angiogenic vessels, have been identified and can be used as targets for molecular imaging of cancer. With the introduction of ultrasound contrast agents that can be targeted to those molecular markers, targeted contrast-enhanced ultrasound (molecular ultrasound) imaging has become an attractive imaging modality to non-invasively assess tumor angiogenesis at the molecular level. The advantages of molecular ultrasound imaging such as high temporal and spatial res... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3670893 Mon, 14 Jun 2010 14:08:02 +0100 3670893 http://www.medworm.com/index.php?rid=3655288&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fym7352243j2r498t%2F Content Type Journal ArticleCategory ErratumDOI 10.1007/s10456-010-9171-3Authors Jennifer Delli Carpini, Memorial Sloan-Kettering Cancer Center Breast Service, Department of Surgery 1275 York Avenue New York NY 10065 USAAmer K. Karam, Memorial Sloan-Kettering Cancer Center Breast Service, Department of Surgery 1275 York Avenue New York NY 10065 USALeslie Montgomery, Memorial Sloan-Kettering Cancer Center Breast Service, Department of Surgery 1275 York Avenue New York NY 10065 USA Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3655288 Thu, 10 Jun 2010 17:40:45 +0100 3655288 http://www.medworm.com/index.php?rid=3651910&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fwu17108730x56187%2F Abstract  Atherosclerosis is a progressive systemic disease of the large arteries characterized by the formation of plaques in the vessel wall. Despite our knowledge of its pathogenesis, many vulnerable plaques still remain undiagnosed while in their asymptomatic phase and manifest for the first time with dramatic clinical events, such as stroke or myocardial infarction. In recent years, it is becoming clearer that sudden clinical events do not necessarily correlate with the degree of luminal obstruction caused by lesions, but rather with plaque composition. In particular, the degree of plaque inflammation is important in the pathogenesis of atherosclerosis and is considered a good marker of high-risk/vulnerable plaques. The presence of inflammatory infiltrate and plaque ne... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3651910 Mon, 07 Jun 2010 16:14:00 +0100 3651910 http://www.medworm.com/index.php?rid=3628998&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq27u43t243gh6444%2F Abstract  During the early stages of angiogenesis, following stimulation of endothelial cells by vascular endothelial growth factor (VEGF), the vascular wall is breached, allowing high molecular weight proteins to leak from the vessels to the interstitial space. This hallmark of angiogenesis results in deposition of a provisional matrix, elevation of the interstitial pressure and induction of interstitial convection. Albumin, the major plasma protein appears to be an innocent bystander that is significantly affected by these changes, and thus can be used as a biomarker for vascular permeability associated with angiogenesis. Traditionally, albumin leak in superficial organs was followed by colorimetry or morphometry with the use of albumin binding vital dyes. Over the last y... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3628998 Fri, 28 May 2010 18:02:48 +0100 3628998 http://www.medworm.com/index.php?rid=3563354&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy33j2508467q5062%2F In this study, we infected SeV to the MSCs in vitro; and injected MSCs modified with SeV harboring human angiopoietin-1 gene (SeVhAng-1) into the ischemic limb of rats in vivo. We found SeV had high transductive efficiency to the MSCs. Both MSCs and SeVhAng-1-modified MSCs improved the blood flow recovery and increased the capillary density of the ischemic limb, compared with the control. However, in contrast to MSCs, SeVhAng-1-modified MSCs had a better improvement of blood flow recovery in the ischemic limb. We further found the ischemic limb injected with SeVhAng-1-modified MSCs had strong expression of p-Akt, which improved survival of MSCs injected into the ischemic limb. This indicated SeVhAng-1 modification enhanced angiogenetic effect of MSCs by both angiogenesis and cell pr... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3563354 Tue, 11 May 2010 08:12:35 +0100 3563354 http://www.medworm.com/index.php?rid=3546781&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F693t526x27t42rtk%2F Abstract  In recent years, molecular imaging gained significant importance in biomedical research. Optical imaging developed into a modality which enables the visualization and quantification of all kinds of cellular processes and cancerous cell growth in small animals. Novel gene reporter mice and cell lines and the development of targeted and cleavable fluorescent “smart” probes form a powerful imaging toolbox. The development of systems collecting tomographic bioluminescence and fluorescence data enabled even more spatial accuracy and more quantitative measurements. Here we describe various bioluminescent and fluorescent gene reporter models and probes that can be used to specifically image and quantify neovascularization or the angiogenic process itself. Conten... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3546781 Fri, 07 May 2010 08:45:33 +0100 3546781 http://www.medworm.com/index.php?rid=3502693&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F00712680170w0r2r%2F Abstract  Molecular imaging agents are extending the potential of noninvasive medical diagnosis from basic gross anatomical descriptions to complicated phenotypic characterizations based upon the recognition of unique cell-surface biochemical signatures. Although originally the purview of nuclear medicine, “molecular imaging” is now studied in conjunction with all clinically relevant imaging modalities. Of the myriad of particles that have emerged as prospective candidates for clinical translation, perfluorocarbon nanoparticles offer great potential for combining targeted imaging with drug delivery, much like the “magic bullet” envisioned by Paul Ehrlich 100 years ago. Perfluorocarbon nanoparticles, once studied in Phase III clinical trials as blood substitutes,... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3502693 Thu, 22 Apr 2010 09:12:04 +0100 3502693 http://www.medworm.com/index.php?rid=3482509&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq4l502811342182k%2F Abstract  Angiogenesis is essential for tumor growth and metastatic potential and for that reason considered an important target for tumor treatment. Noninvasive imaging technologies, capable of visualizing tumor angiogenesis and evaluating the efficacy of angiostatic therapies, are therefore becoming increasingly important. Among the various imaging modalities, magnetic resonance imaging (MRI) is characterized by a superb spatial resolution and anatomical soft-tissue contrast. Revolutionary advances in contrast agent chemistry have delivered versatile angiogenesis-specific molecular MRI contrast agents. In this paper, we review recent advances in the preclinical application of paramagnetic and fluorescent liposomes for noninvasive visualization of the molecular processes i... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3482509 Wed, 14 Apr 2010 06:54:39 +0100 3482509 http://www.medworm.com/index.php?rid=3467201&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc50k6812k30371hx%2F Abstract  Squamous cell carcinoma of the head and neck (HNSCC) is the seventh most common cancer in the United States. Angiogenesis, the process by which new blood vessels are formed, is an essential element at the basis of both tumor growth and metastases. This review discusses pertinent aspects of the role of imaging modalities in assessing angiogenesis and anti-angiogenic therapy in advanced HNSCC. Content Type Journal ArticleDOI 10.1007/s10456-010-9167-zAuthors Jacobus F. A. Jansen, Memorial Sloan-Kettering Cancer Center Department of Medical Physics 1275 York Avenue New York NY 10065 USAJason A. Koutcher, Memorial Sloan-Kettering Cancer Center Department of Medical Physics 1275 York Avenue New York NY 10065 USAAmita Shukla-Dave, Memorial Sloan-Kettering Cancer Cent... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3467201 Sun, 11 Apr 2010 05:50:59 +0100 3467201 http://www.medworm.com/index.php?rid=3369765&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4t2977k2t7267280%2F Abstract  Tumor neovascularization is a complex process that plays a crucial role in the development of many different types of cancer. Vascular endothelial growth factor (VEGF) is a potent mitogen that is involved with mitogenesis, angiogenesis, endothelial survival, and the induction of hematopoiesis. By increasing vascular permeability in endothelial cells, it helps tumors recruit wound-healing proteins fibrin and fibrinogen from the plasma, suggesting that tumor formation is a process of abnormal wound healing dependent on the ability to generate a blood supply. The human female reproductive tract is highly dependent on VEGF for normal functions such as endometrial proliferation and development of the corpus luteum. The unique influence of female sex steroid hormones on... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3369765 Sun, 14 Mar 2010 12:26:49 +0100 3369765 http://www.medworm.com/index.php?rid=3362077&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3qw4314488701m74%2F Abstract  Von Hippel-Lindau (VHL) inactivation develops a tumor syndrome characterized by highly vascularized tumors as a result of hypoxia inducible factors (HIF) stabilization. The most common manifestation is the development of hemangioblastomas typically located in the central nervous system and other organs including the liver. PTEN (Phosphatase and tension homologue deleted on chromosome 10) inactivation also upregulates HIF-1α and may take part in promoting vascular lesions in tumors. The coordinate effect of loss of these tumor suppressors on HIF levels, and the subsequent effect on vascular lesion formation would elucidate the potential for mechanisms to modify HIF dosage supplementally and impact tumor phenotype. We therefore employed models of somatic conditional... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3362077 Thu, 11 Mar 2010 02:45:21 +0100 3362077 http://www.medworm.com/index.php?rid=3287231&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb12425g72521524p%2F Abstract  Vascular endothelial cells undergo many molecular changes during pathological processes such as inflammation and tumour development. Tumours such as malignant lymphomas affecting bone marrow are dependent on interactions with endothelial cells for (1) site-specific homing and (2) tumour-induced angiogenesis. Modifications in glycosylation are responsible for fine-tuning of distinct endothelial surface receptors. In order to gain a comprehensive insight into the regulation of the endothelial glycome, comprising genes encoding for sugar transporters (sugar s/t), glycosyltransferases (GT), glycan-degrading enzymes (GD) and lectins (GBP), we performed gene profiling analysis of the human bone marrow–derived microvascular endothelial cell line HBMEC-60 that resembles ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3287231 Wed, 17 Feb 2010 06:59:05 +0100 3287231 http://www.medworm.com/index.php?rid=3171306&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc88351j340j25120%2F Conclusion  HemSCs have distinct Notch expression patterns from differentiated HemECs and from normal human endothelial cells. Notch3 is expressed in HemSCs, while Notch1, Notch4, and Jagged-1 have higher expression levels in HemECs. Notch3 was localized to the interstitial cells outside of the nascent vascular channels in proliferating IH tissue sections, but became more apparent in the perivascular cells in involuting IH. In summary, the pattern of Notch gene expression mirrors the progression from immature cells to endothelial-lined vascular channels (i.e., endothelial differentiation) that characterizes the growth and involution of IH. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10456-009-9161-5Authors June K. Wu, Columbia University, College... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3171306 Tue, 12 Jan 2010 18:20:49 +0100 3171306 http://www.medworm.com/index.php?rid=3088735&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe75714u240323749%2F Abstract  Tyrosine kinases are important cellular signaling proteins that have a variety of biological activities including cell proliferation and migration. Multiple kinases are involved in angiogenesis, including receptor tyrosine kinases such as the vascular endothelial growth factor receptor. Inhibition of angiogenic tyrosine kinases has been developed as a systemic treatment strategy for cancer. Three anti-angiogenic tyrosine kinase inhibitors (TKIs), sunitinib, sorafenib and pazopanib, with differential binding capacities to angiogenic kinases were recently approved for treatment of patients with advanced cancer (renal cell cancer, gastro-intestinal stromal tumors, and hepatocellular cancer). Many other anti-angiogenic TKIs are being studied in phase I-III clinical tr... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=3088735 Fri, 11 Dec 2009 06:53:53 +0100 3088735 http://www.medworm.com/index.php?rid=2988478&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft230706g24vt3493%2F Abstract  Pigment epithelium–derived factor (PEDF) is a well-known protease inhibitor for angiogenesis in the eye, suggesting that loss of PEDF in eye is implicated in the pathogenesis of proliferative diabetic retinopathy. Since the role of PEDF in diabetic retinopathy is unclear, the effect of PEDF on different types of cells constituting the blood vessel has to be checked. Here, we have investigated the effects of PEDF under hyperglycemic conditions in retinal pericytes, isolated from goat’s eye and used to analyze the signaling pathway involved. High glucose increased the apoptotic cell death and intracellular reactive oxygen species generation, which was blocked on the addition of PEDF. PEDF was found to inhibit the apoptotic cell death and protect the cells via ac... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2988478 Wed, 11 Nov 2009 07:18:31 +0100 2988478 http://www.medworm.com/index.php?rid=2954000&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Faq7245rj36430672%2F In this study, we tested the anti-angiogenic properties of pyrrolidine dithiocarbamate (PDTC), a NF-κB inhibitor, using established in vitro and ex vivo assays in human umbilical vein endothelial cells (HUVEC) and human metastatic RCC cell lines (ACHN and SN12K1). In vitro, PDTC inhibited proliferation, capillary tube formation, invasion and trans-differentiation of HUVEC. Ex vivo, PDTC blocked vessel sprouting from aortic explants and disrupted the integrity of pre-formed vessels. PDTC also inhibited the adhesion of HUVEC and RCC cells to substratum and inhibited their invasion. PDTC inhibited RCC-induced proliferation of HUVEC. Protein microarray demonstrated heterogenic actions in each cell line: in HUVEC, epidermal growth factor was significantly decreased; in ACHN, basic fibrob... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2954000 Fri, 30 Oct 2009 18:32:00 +0100 2954000 http://www.medworm.com/index.php?rid=2836053&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft425005286131382%2F Abstract  Chemokine (C-C motif) receptor-2 (CCR2) regulates arteriogenesis and angiogenesis, facilitating the MCP-1-dependent recruitment of growth factor-secreting bone marrow-derived cells (BMCs). Here, we tested the hypothesis that the BMC-specific expression of CCR2 is also required for new arteriole formation via capillary arterialization. Following non-ischemic saphenous artery occlusion, we measured the following in gracilis muscles: monocyte chemotactic protein-1 (MCP-1) in wild-type (WT) C57Bl/6J mice by ELISA, and capillary arterialization in WT–WT and CCR2−/−–WT (donor–host) bone marrow chimeric mice, as well as BMC transdifferentiation in EGFP+–WT mice, by smooth muscle (SM) α-actin immunochemistry. MCP-1 levels were significantly elevated 1 day ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2836053 Thu, 24 Sep 2009 05:53:44 +0100 2836053 http://www.medworm.com/index.php?rid=2812148&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F17042830560x0un2%2F Abstract  Intermittent Hypoxia (IH) that develops in neovascularized solid tumours has been described to positively influence the tumour growth by modulating the behaviour of cancer cells as well as of endothelial cells. However, the molecular mechanisms regulated by IH still remain poorly understood. In this work, the effects of IH were investigated on endothelial cells by a proteomic approach. Protein abundance variations were studied using fluorescent 2D-Differential in Gel Electrophoresis (2D-DIGE). Amongst the proteins of which the abundance varied under IH, NDRG1 and CRK-I/II were identified by mass spectrometry. These proteins have already been described to influence cancer cell migration as well as the angiogenic processes in solid tumours. Since an increase in endo... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2812148 Thu, 17 Sep 2009 12:32:08 +0100 2812148 http://www.medworm.com/index.php?rid=2812149&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy61t41w9v6457436%2F Abstract  Rodent models of retinal angiogenesis play a pivotal role in angiogenesis research. These models are a window to developmental angiogenesis, to pathological retinopathy, and are also in vivo tools for anti-angiogenic drug screening in cancer and ophthalmic research. The mouse model of oxygen-induced retinopathy (OIR) has emerged as one of the leading in vivo models for these purposes. Many of the animal studies that laid the foundation for the recent breakthrough of anti-angiogenic treatments into clinical practice were performed in the OIR model. However, readouts from the OIR model have been time-consuming and can vary depending on user experience. Here, we present a computer-aided quantification method that is characterized by (i) significantly improve... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2812149 Wed, 16 Sep 2009 13:08:57 +0100 2812149 http://www.medworm.com/index.php?rid=2708457&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq328344378m7t333%2F Conclusion  Our study, for the first time, shows that targeting of the PI3K pathway using nanoparticles can offer an attractive strategy for inhibiting tumor angiogenesis. Content Type Journal ArticleDOI 10.1007/s10456-009-9154-4Authors Rania Harfouche, Brigham and Women’s Hospital, Harvard Medical School Harvard-MIT Division of Health Sciences and Technology Boston MA 02115 USASudipta Basu, Brigham and Women’s Hospital, Harvard Medical School Harvard-MIT Division of Health Sciences and Technology Boston MA 02115 USAShivani Soni, Brigham and Women’s Hospital, Harvard Medical School Harvard-MIT Division of Health Sciences and Technology Boston MA 02115 USADirk M. Hentschel, Brigham and Women’s Hospital, Harvard Medical School Department of Medicine Boston MA 0211... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2708457 Fri, 14 Aug 2009 16:54:33 +0100 2708457 http://www.medworm.com/index.php?rid=2681416&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk920553u72606238%2F Abstract  Retinal angiogenesis in diabetes may lead to visual impairment and even irreversible blindness in people of working age group worldwide. The main pathological feature of proliferative diabetic retinopathy (PDR) is hypoxia, and overproduction of growth factors like vascular endothelial growth factor (VEGF) and erythropoietin (Epo). This results in pathological proliferation of retinal endothelial cells (RECs), leading to new vessel formation (angiogenesis). Inhibition of angiogenesis is a promising strategy for treatment of PDR and other retinal neovascular disorders. Pigment epithelium-derived factor (PEDF), a 50-kDa protein secreted by retinal pigment epithelium, inhibits the growth of new blood vessel induced in the eye in a variety of ways with a yet elusive me... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2681416 Thu, 06 Aug 2009 08:21:45 +0100 2681416 http://www.medworm.com/index.php?rid=2504195&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fbq294607p3455221%2F Abstract  Therapeutic angiogenesis is a promising strategy to promote the formation of new or collateral vessels for tissue regeneration and repair. Since changes in tissue oxygen concentrations are known to stimulate numerous cell functions, these studies have focused on the oxygen microenvironment and its role on the angiogenic potential of endothelial cells. We analyzed the proangiogenic potential of human endothelial colony-forming cells (hECFCs), a highly proliferative population of circulating endothelial progenitor cells, and compared outcomes to human dermal microvascular cells (HMVECs) under oxygen tensions ranging from 1% to 21% O2, representative of ischemic or healthy tissues and standard culture conditions. Compared to HMVECs, hECFCs (1) exhibited significantly... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2504195 Mon, 22 Jun 2009 06:38:23 +0100 2504195 http://www.medworm.com/index.php?rid=2504196&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj513lj6h2550wg71%2F Abstract  Angiogenesis is a complex process that relies on a variety of growth factors and signaling pathways to stimulate endothelial cell responses and establish functional blood vessels. Signaling through the vascular endothelial growth factor (VEGF) receptors is an important mediator of angiogenesis, a hallmark of tumor growth and metastasis. Inhibition of signaling through VEGF has been clinically validated with FDA-approvals of bevacizumab, sorafenib, and suntinib. Our goal was to discover an orally available, selective VEGFR-2 inhibitor. A novel oxime, 1-{4-[6-amino-5-(methoxyimino-methyl)-pyrimidin-4-yloxy]-2-chloro-phenyl}-3-ethyl-urea (JNJ-38158471), was identified as a potent and selective inhibitor of VEGFR-2. While JNJ-38158471 shares some structure features wi... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2504196 Mon, 22 Jun 2009 06:38:21 +0100 2504196 http://www.medworm.com/index.php?rid=2439869&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fwj4282u523117211%2F Content Type Journal ArticleCategory ErratumDOI 10.1007/s10456-009-9149-1Authors Guido Serini, Institute for Cancer Research and Treatment 10060 Torino Candiolo ItalyFederica Maione, Institute for Cancer Research and Treatment 10060 Torino Candiolo ItalyEnrico Giraudo, Institute for Cancer Research and Treatment 10060 Torino Candiolo ItalyFederico Bussolino, Institute for Cancer Research and Treatment 10060 Torino Candiolo Italy Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2439869 Wed, 27 May 2009 06:16:08 +0100 2439869 http://www.medworm.com/index.php?rid=2422787&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw0u608217817u225%2F Abstract  The fibrinous exudate of a wound or tumor stroma facilitates angiogenesis. We studied the involvement of RGD-binding integrins during tube formation in human plasma-derived fibrin clots and human purified fibrin matrices. Capillary-like tube formation by human microvascular endothelial cells in a 3D plasma-derived fibrinous matrix was induced by FGF-2 and TNF-α and depended largely on cell-bound u-PA and plasmin activities. While tube formation was minimally affected by the addition of either the αvβ3-integrin inhibiting mAb LM609 or the α5-integrin inhibiting mAb IIA1, the general RGD-antagonist echistatin completely inhibited this process. Remarkably, when αvβ3- and α5β1-integrins were inhibited simultaneously, tube formation was reduced by 78%. It was a... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2422787 Sat, 16 May 2009 05:59:23 +0100 2422787 http://www.medworm.com/index.php?rid=2422786&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F66767lt232287588%2F Abstracts  The Angiopoietin/Tie system acts as a vascular specific ligand/receptor system to control endothelial cell survival and vascular maturation. The Angiopoietin family includes four ligands (Angiopoietin-1, Angiopoietin-2 and Angiopoietin-3/4) and two corresponding tyrosine kinase receptors (Tie1 and Tie2). Ang-1 and Ang-2 are specific ligands of Tie2 binding the receptor with similar affinity. Tie2 activation promotes vessel assembly and maturation by mediating survival signals for endothelial cells and regulating the recruitment of mural cells. Ang-1 acts in a paracrine agonistic manner inducing Tie2 phosphorylation and subsequent vessel stabilization. In contrast, Ang-2 is produced by endothelial cells and acts as an autocrine antagonist of Ang-1-mediated Tie2 ac... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2422786 Sat, 16 May 2009 05:59:23 +0100 2422786 http://www.medworm.com/index.php?rid=2422788&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ful720150m88445q7%2F In conclusion, we provide evidence that Wnt2 acts as an angiogenic factor for non-sinusoidal endothelium in vitro and in vivo whose target genes undergo complex regulation by the tissue microenvironment. Content Type Journal ArticleCategory Original paperDOI 10.1007/s10456-009-9145-5Authors Diana Klein, University Medical Center Mannheim, Ruprecht-Karl University Heidelberg Department of Dermatology, Venereology and Allergology Theodor-Kutzer-Ufer 1-3 68167 Mannheim GermanyAlexandra Demory, University Medical Center Mannheim, Ruprecht-Karl University Heidelberg Department of Dermatology, Venereology and Allergology Theodor-Kutzer-Ufer 1-3 68167 Mannheim GermanyFrancis Peyre, University Medical Center Mannheim, Ruprecht-Karl University Heidelberg Department of Dermatology, Venereology... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2422788 Fri, 15 May 2009 06:13:10 +0100 2422788 http://www.medworm.com/index.php?rid=2413373&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fnt753l0722607p7h%2F Abstract  Infantile hemangioma is a vascular tumor that occurs in 5–10% of infants of European descent. A defining feature of infantile hemangioma is the dramatic growth and development into a disorganized mass of blood vessels. Subsequently, a slow spontaneous involution begins around 1 year of age and continues for 4–6 years. The growth and involution of infantile hemangioma is very different from other vascular tumors and vascular malformations, which do not regress and can occur at any time during childhood or adult life. Much has been learned from careful study of the tissue morphology and gene expression patterns during the life-cycle of hemangioma. Tissue explants and tumor-derived cell populations have provided further insight to unravel the cellular an... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2413373 Sun, 10 May 2009 05:46:44 +0100 2413373 http://www.medworm.com/index.php?rid=2386555&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F513mq8v754287618%2F Abstract  It has been more than 20 years since it was first demonstrated that endothelial cells will rapidly form capillary-like structures in vitro when plated on top of a reconstituted basement membrane extracellular matrix (BME, Matrigel, EHS matrix, etc.). Subsequently, this morphological differentiation has been demonstrated with a variety of endothelial cells; with endothelial progenitor cells; and with transformed/immortalized endothelial cells. The differentiation process involves several steps in blood vessel formation, including cell adhesion, migration, alignment, protease secretion, and tubule formation. Because the formation of vessel structures is rapid and quantifiable, endothelial cell differentiation on basement membrane has found numerous applications ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2386555 Tue, 28 Apr 2009 13:37:33 +0100 2386555 http://www.medworm.com/index.php?rid=2340211&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj7478700r74057q7%2F Abstract  Fenofibrate is a synthetic ligand for the nuclear receptor peroxisome proliferator-activated receptor (PPAR) alpha and has been widely used in the treatment of metabolic disorders, especially hyperlipemia, due to its lipid-lowering effect. The molecular mechanism of lipid-lowering is relatively well defined: an activated PPARα forms a PPAR–RXR heterodimer and this regulates the transcription of genes involved in energy metabolism by binding to PPAR response elements in their promoter regions, so-called “trans-activation”. In addition, fenofibrate also has anti-inflammatory and anti-athrogenic effects in vascular endothelial and smooth muscle cells. We have limited information about the anti-inflammatory mechanism of fenofibrate; however, “trans-repression�... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2340211 Thu, 09 Apr 2009 06:49:38 +0100 2340211 http://www.medworm.com/index.php?rid=2295664&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy2143075l961222n%2F Abstract  Among the key effects of fluid shear stress on vascular endothelial cells is modulation of gene expression. Promoter sequences termed shear stress response elements (SSREs) mediate the responsiveness of endothelial genes to shear stress. While previous studies showed that shear stress responsiveness is mediated by a single SSRE, these endogenous promoters often encode for multiple SSREs. Moreover, hybrid promoters encoding a single SSRE rarely respond to shear stress at the same magnitude as the endogenous promoter. Thus, to better understand the interplay between the various SSREs, and between SSREs and endothelial-specific sequences (ESS), we generated a series of constructs regulated by SSREs cassettes alone, or in combination with ESS, and tested their respons... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2295664 Thu, 26 Mar 2009 08:29:45 +0100 2295664 http://www.medworm.com/index.php?rid=2295667&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx1082003157673p4%2F In this study, we constructed a human Ang-1-expressing Sendai viral vector (SeVhAng-1) and injected it into the ischemic limb of rats. We found that SeVhAng-1 improved the blood flow recovery and increased the capillary density of the ischemic limb, compared with the controls. We also found that SeVhAng-1 increased p-Akt during the early period of limb ischemia, and decreased apoptosis in ischemic limb. It suggests that SeVhAng-1 may serve as a potential therapeutic tool in ischemic limb disease. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10456-009-9144-6Authors Jianhua Huang, Sapporo Medical University Department of Molecular Medicine South 1, West 17, Chuo-ku Sapporo 060-8556 JapanMakoto Inoue, DNAVEC Research Corporation Tsukuba, Ibaraki JapanMamoru Hasegaw... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2295667 Thu, 26 Mar 2009 08:29:43 +0100 2295667 http://www.medworm.com/index.php?rid=2258301&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv5nw351u42548312%2F This article discusses several possible mechanisms reported by different labs which mediate formation of the complex between VEGFR-2 and αvβ3 on endothelial cells. The pathological consequences and regulatory events involved in this receptor cross-talk are also presented. Content Type Journal ArticleCategory Review PaperDOI 10.1007/s10456-009-9141-9Authors Payaningal R. Somanath, The Cleveland Clinic Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, NB50, Lerner Research Institute 9500 Euclid Avenue Cleveland OH 44195 USANikolay L. Malinin, The Cleveland Clinic Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, NB50, Lerner Research Institute 9500 Euclid Avenue Cleveland OH 44195 USATatiana... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2258301 Sun, 08 Mar 2009 09:55:23 +0100 2258301 http://www.medworm.com/index.php?rid=2249050&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fqg718822141qv334%2F Abstract  Semaphorins belong to a large family of proteins well-conserved along evolution from viruses to mammalians. Secreted and membrane-bound semaphorins participate in a wide range of biological phenomena including development and regeneration of nervous system, cardiovascular development, and immune system activities. Different classes of semaphorins are bifunctional and often exert opposite effects (i.e., repellent or attractive) by acting through the plexin receptor family. However, some classes use other membrane receptors and the same plexin-mediated signals may be modulated by co-receptors, in particular neuropilins or some tyrosine kinase receptors. In cancer, semaphorins have both tumor-suppressor and tumor-promoting functions, by acting on both tumor and strom... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2249050 Fri, 06 Mar 2009 11:59:41 +0100 2249050 http://www.medworm.com/index.php?rid=2227092&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd726h2p31x7446uu%2F We report the effects of inhibiting or inducing CLIC4 expression on distinct aspects of endothelial cell behavior in vitro. Our experiments utilized RNA interference to establish cultured human endothelial cell lines with significant reduction of CLIC4 expression, and a CLIC4-expressing lentiviral plasmid was used to establish CLIC4 overexpression in endothelial cells. We observed no effect on cell migration and a modest effect on cell survival. Reduced CLIC4 expression decreased cell proliferation, capillary network formation, capillary-like sprouting, and lumen formation. This suggests that normal endogenous CLIC4 expression is required for angiogenesis and tubulogenesis. Accordingly, increased CLIC4 expression promoted proliferation, network formation, capillary-like sprouting, an... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2227092 Fri, 27 Feb 2009 10:45:48 +0100 2227092 http://www.medworm.com/index.php?rid=2204925&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5442l6k7385l012l%2F Abstract  Nucleolin is specifically transported to the surface of proliferating endothelial cells in vitro and in vivo. In contrast to its well defined functions in the nucleus and cytoplasm, the function of cell surface nucleolin is poorly defined. We have previously identified the nucleolin-binding antibody NCL3 that specifically binds to cell surface nucleolin on angiogenic blood vessels in vivo and is internalized into the cell. Here, we show that NCL3 inhibits endothelial tube formation in vitro as well as angiogenesis in the matrigel plaque assay and subcutaneous tumor models in vivo. Intriguingly, the specific targeting of proliferating endothelial cells by NCL3 in subcutaneous tumor models leads to the normalization of the tumor vasculature and as a result to an inc... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2204925 Thu, 19 Feb 2009 14:23:40 +0100 2204925 http://www.medworm.com/index.php?rid=2200336&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm702j81958567662%2F Abstract  Increasing evidence suggests the importance of bone marrow-derived cells for blood vessel formation (neovascularization) in tumors, which can occur in two mechanisms: angiogenesis and vasculogenesis. Angiogenesis results from proliferation and sprouting of existing blood vessels close to the tumor, while vasculogenesis is believed to arise from recruitment of circulating cells, largely derived from the bone marrow, and de novo clonal formation of blood vessels from these cells. Although bone marrow-derived cells are crucial for neovascularization, current evidence suggests a promotional role of these cells on the existing blood vessels rather than de novo neovascularization in tumors. This is believed to be due to the highly proangiogenic features of these cells. ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2200336 Tue, 17 Feb 2009 13:01:50 +0100 2200336 http://www.medworm.com/index.php?rid=2190451&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk704v1j1788u2600%2F Abstract  Fibronectin is an extracellular matrix protein found only in vertebrate organisms containing endothelium-lined vasculature and is required for cardiovascular development in fish and mice. Fibronectin and its splice variants containing EIIIA and EIIIB domains are highly upregulated around newly developing vasculature during embryogenesis and in pathological conditions including atherosclerosis, cardiac hypertrophy, and tumorigenesis. However, their molecular roles in these processes are not entirely understood. We review genetic studies examining functions of fibronectin and its splice variants during embryonic cardiovascular development, and discuss potential roles of fibronectin in vascular disease and tumor angiogenesis. Content Type Journal ArticleCategory Re... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2190451 Sat, 14 Feb 2009 07:58:29 +0100 2190451 http://www.medworm.com/index.php?rid=2187639&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv2091mm1x6615380%2F We report that bleomycin, mitomycin C and cytoskeletal-disrupting drugs (2-methoxyestradiol, taxol, vincristine, vinblastine, colchicine, nocodazole, and cytochalasin D) exhibit anti-angiogenic activities of varying potency. Bleomycin and the various cytoskeletal-disrupting drugs inhibited endothelial cell migration, while mitomycin C had a marginal effect. Both DNA-damaging and cytoskeletal-disrupting drugs decreased endothelial cell growth in a dose-dependent manner, and this was accompanied by the induction of apoptosis. The growth inhibitory and apoptotic effects of cytoskeletal-disrupting drugs were the most pronounced. We also show that both classes of drugs inhibited capillary-like tube formation in an assay of in vitro angiogenesis, with cytoskeletal-disrupting agents inhibit... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2187639 Fri, 13 Feb 2009 08:29:06 +0100 2187639 http://www.medworm.com/index.php?rid=2187640&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F675l2723r6154006%2F In conclusion, opposite kinetics of two circulating CD34bright cell populations, HPCs and small CECs, were observed in sunitinib-treated RCC patients. The increase in CECs is likely caused by sunitinib targeting of immature tumor vessels. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10456-009-9133-9Authors Laura Vroling, VU University Medical Center Department of Medical Oncology CCA 2-54, De Boelelaan 1117 1081 HV Amsterdam The NetherlandsAstrid A. M. van der Veldt, VU University Medical Center Department of Medical Oncology CCA 2-54, De Boelelaan 1117 1081 HV Amsterdam The NetherlandsRichard R. de Haas, VU University Medical Center Department of Medical Oncology CCA 2-54, De Boelelaan 1117 1081 HV Amsterdam The NetherlandsJohn B. A. G. Haanen, The Netherlands Ca... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2187640 Thu, 12 Feb 2009 07:24:30 +0100 2187640 http://www.medworm.com/index.php?rid=2187641&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fdu2853200h124h27%2F Abstract  Transport in the large and complex bodies of vertebrate organisms is mediated by extensive and highly branched tubular networks that are formed by endothelial cells. Blood vessels are responsible for systemic circulation, while the lymphatic vasculature drains extravasated plasma, proteins, particles, and cells from the interstitium. Endothelial cells of blood vessels and lymphatic vessels can be distinguished by the expression of certain molecular markers, which accompany or even contribute to functional and morphological differences. Even within the blood vessel network, some molecules and pathways selectively mark the endothelium of arteries, veins and capillaries and are thought to contribute to the differentiation of these vessels. Moreover, microvessels can ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2187641 Thu, 12 Feb 2009 07:24:28 +0100 2187641 http://www.medworm.com/index.php?rid=2167455&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd4814g17200h61hv%2F Abstract  New blood vessels arise initially as blood islands in the process known as vasculogenesis or as new capillary segments produced through angiogenesis. Angiogenesis itself encompasses two broad processes, namely sprouting (SA) and intussusceptive (IA) angiogenesis. Primordial capillary plexuses expand through both SA and IA, but subsequent growth and remodeling are achieved through IA. The latter process proceeds through transluminal tissue pillar formation and subsequent vascular splitting, and the direction taken by the pillars delineates IA into overt phases, namely: intussusceptive microvascular growth, intussusceptive arborization, and intussusceptive branching remodeling. Intussusceptive microvascular growth circumscribes the process of initiation of pillar fo... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2167455 Thu, 05 Feb 2009 07:07:23 +0100 2167455 http://www.medworm.com/index.php?rid=2147491&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3122081816744413%2F Abstract  The presence of hypoxia in tumor and its role in promoting angiogenesis are well-established. Recently, in addition to chronic hypoxia, cycling or intermittent hypoxia has also been demonstrated. However, its role in inducing new blood vessel formation is less clear. This work is aimed to investigate whether intermittent hypoxia can induce a pro-angiogenic phenotype in endothelial cells, in vitro. We studied changes in the expression of genes involved in inflammation and angiogenesis under intermittent and chronic hypoxia. We evidenced genes specifically expressed under intermittent hypoxia, suggesting different cell responses induced by intermittent versus chronic hypoxia. An increase in the expression of pro-angiogenic and pro-inflammatory genes under intermitte... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2147491 Thu, 29 Jan 2009 11:09:47 +0100 2147491 http://www.medworm.com/index.php?rid=2141268&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft86k8318227wx0pp%2F Abstract  The function of the endothelium is to provide a network to allow delivery of oxygen and nutrients to tissues throughout the body. This network comprises adjacent endothelial cells that utilize adherens junction proteins such as vascular endothelial cadherin (VE-cadherin) to maintain the appropriate level of vascular permeability. The disruption of VE-cadherin interactions during pathologic settings can lead to excessive vascular leak with adverse effects. Endogenous cell signaling systems have been defined, which help to maintain the proper level of vascular stability. Perhaps the best described system is Angiopoietin-1 (Ang-1). Ang-1 acting through its receptor Tie2 generates a well-described set of signaling events ultimately leading to enhanced vascular stabili... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2141268 Tue, 27 Jan 2009 07:55:03 +0100 2141268 http://www.medworm.com/index.php?rid=2094699&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9451452810082t8v%2F Abstract  The cardiovascular system plays a crucial role in vertebrate development and homeostasis. Several genetic and epigenetic mechanisms are involved in the early development of the vascular system. During embryonal life, blood vessels first appear as the result of vasculogenesis, whereas remodeling of the primary vascular plexus occurs by angiogenesis. Many tissue-derived factors are involved in blood vessel formation and evidence is emerging that endothelial cells themselves represent a source of instructive signals to non-vascular tissue cells during organ development. This review article summarizes our knowledge concerning the principal factors involved in the regulation of vascular morphogenesis. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2094699 Thu, 08 Jan 2009 09:04:38 +0100 2094699 http://www.medworm.com/index.php?rid=2073736&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fqw041617144706t3%2F Conclusions  These results reveal a novel role for HPTPβ in modulating Ang-1-Tie2 signaling and endothelial cell survival. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10456-008-9126-0Authors Orisha K. Yacyshyn, St Michael’s Hospital Terrence Donnelly Heart Centre and Division of Cardiology 30 Bond St. Toronto ON M5B 1W8 CanadaPatrick F. H. Lai, St Michael’s Hospital Terrence Donnelly Heart Centre and Division of Cardiology 30 Bond St. Toronto ON M5B 1W8 CanadaKelly Forse, University of Toronto Human Biology Program Toronto ON M5S 1A8 CanadaKrystyna Teichert-Kuliszewska, St Michael’s Hospital Terrence Donnelly Heart Centre and Division of Cardiology 30 Bond St. Toronto ON M5B 1W8 CanadaPaul Jurasz, St Michael’s Hospital Terrence Donnelly Heart... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2073736 Wed, 31 Dec 2008 10:00:43 +0100 2073736 http://www.medworm.com/index.php?rid=2065168&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6367m64033502553%2F In this study, we assessed the role of OPG and its ligands, receptor activator of nuclear factor-κB ligand (RANKL) and tumor necrosis factor-related apoptosis inducing ligand (TRAIL), in microvessel formation using the rat aortic ring model of angiogenesis. OPG was found to promote a twofold increase in angiogenic sprouting in the aortic ring model, and this effect was inhibited by pre-incubation with a fivefold molar excess of either RANKL or TRAIL. While TRAIL had no effect upon angiogenesis on its own, RANKL was found to potently inhibit basal and vascular endothelial growth factor-induced angiogenesis. OPG increased the rate of endothelial cell proliferation in sprouting microvessels; in contrast, RANKL inhibited proliferation. RANKL was found to induce endothelial apoptosis at ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2065168 Tue, 23 Dec 2008 07:15:55 +0100 2065168 http://www.medworm.com/index.php?rid=2052526&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9t61505m02126v51%2F Abstract  The α subunit of heterotrimeric G13 protein is required for the embryonic angiogenesis (Offermanns et al., Science 275:533–536, 1997). However, the molecular mechanism of Gα13-dependent angiogenesis is not understood. Here, we show that myocyte-specific enhancer factor-2 (MEF2) mediates Gα13-dependent angiogenesis. Our data showed that constitutively activated Gα13Q226L stimulated MEF2-dependent gene transcription. In addition, downregulation of endogenous Gα13 inhibited thrombin-stimulated MEF2-dependent gene transcription in endothelial cells. Both Ca2+/calmodulin-dependent kinase IV (CaMKIV) and histone deacetylase 5 (HDAC5) were involved in Gα13-mediated MEF2-dependent gene transcription. Gα13Q226L also increased Ca2+/calmodulin-independent CaMKIV acti... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2052526 Thu, 18 Dec 2008 07:03:46 +0100 2052526 http://www.medworm.com/index.php?rid=2032962&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2n5745074877u611%2F Abstract  Molecular imaging of angiogenesis is urgently needed for diagnostic purposes such as early detection, monitoring of (angiostatic) therapy and individualized therapy. Multimodality molecular imaging is a promising and refined technique to study tumor angiogenesis, which has so far been largely unexplored due to the lack of suitable multimodal contrast agents. Here, we report on the application of a novel αvβ3-specific quantum dot-based nanoparticle, which has been optimized for both optical and magnetic resonance detection of tumor angiogenesis. Upon intravenous injection of RGD-pQDs in tumor-bearing mice, intravital microscopy allowed the detection of angiogenically activated endothelium at cellular resolution with a small scanning window and limited penetration ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=2032962 Wed, 10 Dec 2008 08:04:05 +0100 2032962 http://www.medworm.com/index.php?rid=1996165&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy3x646277266466l%2F Abstract  Tie2 is a receptor tyrosine kinase that is expressed predominantly in the endothelium and plays key roles in both physiological and pathological angiogenesis. The ligands for Tie2, the angiopoietins (Ang), perform opposing functions in vascular maintenance and angiogenesis; Ang1 regulates vascular quiescence, while Ang2 is thought to promote vascular destabilization and facilitate angiogenesis. However, the mechanisms responsible for these differences are not understood. To begin to elucidate the molecular differences between the angiopoietins, we previously developed a specific RNA aptamer inhibitor of Ang2. Here, we used the same iterative in vitro selection process, termed SELEX (Systematic Evolution of Ligands by EXponential enrichment), to screen a librar... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1996165 Thu, 27 Nov 2008 09:47:10 +0100 1996165 http://www.medworm.com/index.php?rid=1996166&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff25511052g180204%2F Abstract  Following the clinical success of Bevacizumab, a humanized monoclonal antibody that affects the interaction between vascular endothelial growth factor (VEGF) and its receptors, blocking tumor-induced angiogenesis has become one of the most important targets for the development of new cancer therapeutic drugs and procedures. Among the latter, therapeutic vaccination using VEGF as antigen presents itself as very attractive, with the potential of generating not only a growth factor blocking antibody response but also a cellular response against tumor cells and stromal elements, which appear to be a major source of tumor VEGF. In this paper, we report the development of a protein vaccine candidate, based on a human modified VEGF antigen that is expressed at high level... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1996166 Wed, 26 Nov 2008 19:52:23 +0100 1996166 http://www.medworm.com/index.php?rid=1946982&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft6245j7850q0r2m6%2F Abstract  Skin grafts are commonly utilized and proven effective methods of open wound coverage. Revascularization through neoangiogenesis is a pivotal mechanism for skin graft integration and durability. Extracorporeal shock-wave treatment (ESWT) has been demonstrated to accelerate wound repair; however, its mechanism-of-action is unclear. We investigated the role of ESWT in early revascularization of full-thickness skin isografts in a murine model. Cohorts of mice were euthanized and skin grafts were harvested 6 h, 2, 4, and 7 days post grafting ± ESWT. Various aspects of graft neovascularization were measured including gross morphology, quantitative microscopy (vessel number, density), immunohistochemistry (CD31), cDNA SuperArrays for 84 angiogenesis-... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1946982 Sun, 09 Nov 2008 10:05:22 +0100 1946982 http://www.medworm.com/index.php?rid=1888051&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8111753830j41383%2F Abstract  Abundant preclinical and indirect clinical data have for several decades convincingly supported the notion that anti-angiogenesis is an effective strategy for the inhibition of tumor growth. The recent success achieved in patients with metastatic colon carcinoma using a neutralizing antibody directed against vascular endothelial growth factor (VEGF) has translated preclinical optimism into a clinical reality.With this transformation in the field of angiogenesis has come a need for reliable surrogate markers. A surrogate marker by definition serves as a substitute for the underlying process in question, and in the case of angiogenesis, microvessel density (usually in so-called “hot-spots”) has until now been the most widely used parameter. However, this paramet... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1888051 Fri, 17 Oct 2008 09:49:22 +0100 1888051 http://www.medworm.com/index.php?rid=1825371&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg88mk73g02q64081%2F Conclusions  These results suggest that PPARγ ligands, pioglitazone and rosiglitazone, in addition to their important regulatory role in adipogenesis and inflammation, possess anti-angiogenic properties. Thus, PPARγ ligands may be useful in the treatment of diabetic retinopathy, macular degeneration, and other ocular disorders and may lower the risk to develop cancer in diabetic patients. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10456-008-9118-0Authors Ahmad Aljada, Long Island University, C.W. Post. Department of Biomedical Sciences Brookville NY USALaura O’Connor, The Pharmaceutical Research Institute (PRI), Albany College of Pharmacy Albany NY USAYu-Yen Fu, Long Island University, C.W. Post. Department of Biomedical Sciences Brookville NY ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1825371 Tue, 23 Sep 2008 10:06:57 +0100 1825371 http://www.medworm.com/index.php?rid=1806135&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F61t534742m331500%2F Abstract  The planar cell polarity (PCP) pathway is a highly conserved signaling cascade that coordinates both epithelial and axonal morphogenic movements during development. Angiogenesis also involves the growth and migration of polarized cells, although the mechanisms underlying their intercellular communication are poorly understood. Here, using cell culture assays, we demonstrate that inhibition of PCP signaling disrupts endothelial cell growth, polarity, and migration, all of which can be rescued through downstream activation of this pathway by expression of either Daam-1, Diversin or Inversin. Silencing of either Dvl2 or Prickle suppressed endothelial cell proliferation. Moreover, loss of p53 rescues endothelial cell growth arrest but not the migration inhibition caus... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1806135 Wed, 17 Sep 2008 08:33:12 +0100 1806135 http://www.medworm.com/index.php?rid=1782516&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd5534w557130x29x%2F Abstract  Tumor metastasis represents a major problem in the treatment of patients with different cancers. Specific phenotype and behavior of metastatic cells derive from specific molecular mechanisms involved in consecutive steps of the metastatic process. Several in vitro and in vivo experimental models have been utilized, but they cannot completely reproduce and characterize each step of the metastatic process. This review article is focused on the chick embryo chorioallantoic membrane as an in vivo model to study the metastatic process. Content Type Journal ArticleCategory Review PaperDOI 10.1007/s10456-008-9117-1Authors Anca Maria Cimpean, “Victor Babes” University of Medicine and Pharmacy Timisoara Department of Histology Timisoara RomaniaDomenic... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1782516 Tue, 09 Sep 2008 11:17:24 +0100 1782516 http://www.medworm.com/index.php?rid=1749980&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy544t24p2816qmn2%2F We examined the effects of FTI treatment on a range of assays designed to pick apart the individual functions of endothelial cells during angiogenesis. We found that FTIs inhibit endothelial cell proliferation, causing a failure of mitosis and accumulation of binucleate cells. FTIs also block the directional migration of endothelial cells toward VEGF, the major pro-angiogenic factor in adult tissues. In a co-culture assay of angiogenesis, FTI treatment significantly inhibits tube formation, but has no effect on pre-existing structures. Defects in tube formation could be replicated by specific targeting of endothelial cell farnesyltransferase using RNA interference. Our data show that FTIs directly target endothelial cells in angiogenesis, explaining previous in vivo findings. Im... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1749980 Sat, 30 Aug 2008 10:22:59 +0100 1749980 http://www.medworm.com/index.php?rid=1537803&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd515336763l87708%2F Abstract  The placental growth factor (PlGF) is an angiogenic protein belonging to the vascular endothelial growth factor (VEGF) family, which was discovered in 1991 by an Italian scientist, Maria Graziella Persico. Dr Persico cloned and purified PlGF and determined its structure by crystallography resolution. Furthermore, she identified VEGF receptor-1 (VEGFR-1) as the receptor for PlGF, and in collaboration with Dr Peter Carmeliet in Leuven, she generated evidence that loss of PlGF does not affect development, reproduction, or postnatal life. PlGF is expressed primarily in the placenta and is up-regulated in several pathological conditions, although its role is still controversial. Some data in literature reported that PlGF enhances pathological angiogenesis by initiating... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1537803 Sat, 21 Jun 2008 07:06:53 +0100 1537803 http://www.medworm.com/index.php?rid=1475057&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F11j5177662767176%2F Abstract  Lysophosphatidic acid (LPA) is a simple lipid with many important biological functions such as the regulation of cellular proliferation, cellular migration, differentiation, and suppression of apoptosis. Although a direct angiogenic effect of LPA has not been reported to date, there are indications that LPA promotes angiogenesis. In addition, LPA is a chemoattractant for cultured endothelial cells and promotes barrier function in such cultures [1]. To test the hypothesis that LPA is angiogenic, we used the chicken chorio-allantoic membrane (CAM) assay. Sequence analysis of the cloned, full-length chicken LPA receptor cDNAs revealed three receptor types that are orthologous to the mammalian LPA1, LPA2, and LPA3 receptors. We document herein that LPA is angiogenic in... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1475057 Tue, 27 May 2008 05:52:45 +0100 1475057 http://www.medworm.com/index.php?rid=1463642&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw3338h4101026803%2F Abstract  Akt is a pivotal signaling molecule involved in the regulation of angiogenesis. In order to further elucidate the role of Akt1 in blood vessel development, a tetracycline-regulated transgenic system was utilized to conditionally activate Akt1 signaling in endothelial cells to examine transcript expression changes associated with angiogenesis in the heart. Induction of Akt1 over the course of 6 weeks led to a 33% increase in capillary density without affecting overall heart growth. Transcript expression profiles in the hearts were analyzed with an Affymetrix GeneChip Mouse Expression Set 430 2.0, which represents approximately 45,000 cDNAs and ESTs. A total of 248 transcripts were differentially expressed between transgenic and control mice (fold change &... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1463642 Tue, 20 May 2008 06:00:04 +0100 1463642 http://www.medworm.com/index.php?rid=1380474&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F754611072274711t%2F Abstract  Previous in vivo and in vitro studies have shown that Akt1 serves as a crucial regulator of vascular maturation, extracellular matrix composition, and angiogenesis in tumors. Hence, we hypothesized that Akt1 may be necessary for other angiogenesis-dependent processes, including wound healing. Using Akt1 −/− and Akt2 −/− mice, we demonstrate that deficiency of Akt1, but not Akt2, results in impaired assembly of collagen in skin wounds and around the blood vessels. Wounds in Akt1 −/− mice, but not in Akt2 −/− mice, were characterized by reduced vascular area as well as impaired vascular maturation as evidenced by reduced smooth muscle cell recruitment. Expression level of a major angiogenic growth factor, VEGF, was significantly lower in ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1380474 Wed, 16 Apr 2008 14:55:13 +0100 1380474 http://www.medworm.com/index.php?rid=1351917&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm2l008740n6u124l%2F Abstract  Previous studies from our laboratory have demonstrated that thyroid hormones play a key role in cancer progression. In addition, a deaminated form, tetraiodothyroacetic acid (tetrac), that antagonizes the proliferative action of these hormones was found to possess anti-cancer functions through its ability to inhibit cellular proliferation and angiogenesis. The present study was undertaken to investigate whether tetrac could also suppress the development of drug resistance, known as a causative factor of disease relapse. Tetrac was shown to enhance cellular response in vitro to doxorubicin, etoposide, cisplatin, and trichostatin A in resistant tumor cell lines derived from neuroblastoma, osteosarcoma, and breast cancer. The mechanism of action of tetrac did not inv... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1351917 Thu, 03 Apr 2008 08:16:23 +0100 1351917 http://www.medworm.com/index.php?rid=1310918&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu7603176423130h0%2F Abstract  We previously demonstrated that bone marrow (BM) cells migrate to Ewing’s tumors and differentiate into endothelial cells within the tumor vasculature. Recent evidence suggests that the roles of BM cells in tumors are more diverse. We investigated whether non-endothelial cell types critical for tumor vessel development are also derived from migrated BM cells. We utilized BM transplantation with GFP+ transgenic mice as BM donors and nude mice as recipients to track the fate of migrated BM cells. After engraftment, we injected recipient mice either subcutaneously or intramuscularly with Ewing’s sarcoma cells. We labeled functional tumor vessels using intravascular perfusion staining with tomato lectin. We assessed BM cell recruitment/differentiation within the tu... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1310918 Sun, 16 Mar 2008 05:43:45 +0100 1310918 http://www.medworm.com/index.php?rid=1300223&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv41v55k7175282qg%2F Abstract  Angiogenesis, the growth of new blood vessels from the pre-existing vasculature is of physiological and pathological importance. We have investigated the anti-angiogenic potential of two naturally occurring sesterterpenes, leucosesterterpenone (compound 1) and leucosterlactone (compound 2) isolated from the Himalayan plant Leucosceptrum canum and identified as having biological activity in preliminary screening. Compound 1 inhibited fibroblast growth factor-2-induced proliferation, migration in a wounding assay, chemotaxis and tube formation with small vessel (human dermal) and large vessel (bovine aortic) endothelial cells while compound 2 was largely inactive. Both compounds were also active in an in vivo angiogenic model using the chick chorioallantoic membra... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1300223 Tue, 11 Mar 2008 08:29:26 +0100 1300223 http://www.medworm.com/index.php?rid=1294251&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp817440xv0722022%2F Abstract  Angiogenesis is mostly an adaptive response to tissue hypoxia, which occurs under a wide variety of situations ranging from embryonic development to tumor growth. In general, angiogenesis is dependent on the accumulation of hypoxia inducible factors (HIFs), which are heterodimeric transcription factors of α and β subunits. Under normoxia, HIF heterodimers are not abundantly present due to oxygen dependent hydroxylation, polyubiquitination, and proteasomal degradation of α subunits. Under hypoxia, however, α subunits are stabilized and form heterodimers with HIF-1β which is not subject to oxygen dependent regulation. The accumulation of HIFs under hypoxia allows them to activate the expression of many angiogenic genes and therefore initiates the angiogenic pro... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1294251 Mon, 10 Mar 2008 05:33:21 +0100 1294251 http://www.medworm.com/index.php?rid=1290935&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk9301027022w2487%2F Abstract  The aim of the present study was to monitor photodynamic angioocclusion with verteporfin in capillaries. Details of this process were recorded under a microscope in real-time using a high-sensitivity video camera. A procedure was developed based on intravenous (i.v.) injection of a light-activated drug, Visudyne®, into the chorioallantoic membrane (CAM) of a 12-day-old chicken embryo. The effect of light activation was probed after 24 h by i.v. injection of a fluorescent dye (FITC dextran), and analysis of its fluorescence distribution. The angioocclusive effect was graded based on the size of the occluded vessels, and these results were compared with clinical observations. The time-resolved thrombus formation taking place in a fraction of the field of view w... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1290935 Fri, 07 Mar 2008 06:56:59 +0100 1290935 http://www.medworm.com/index.php?rid=1282176&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff5l298m230072627%2F Content Type Journal ArticleCategory ObituaryDOI 10.1007/s10456-008-9103-7Authors Joyce Bischoff, Karp Family Research Laboratories Vascular Biology Program, Children’s Hospital Boston 300 Longwood Ave. Boston MA 02115 USAArjan W. Griffioen, University Hospital Maastricht Department of Pathology 6202 AZ Maastricht The Netherlands Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1282176 Tue, 04 Mar 2008 08:31:59 +0100 1282176 http://www.medworm.com/index.php?rid=1265076&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ffq168r8408267215%2F This article reviews the role of Hedgehog signalling in both embryonic and postnatal vascular development. The temporal importance of a window of hedgehog dependent angiogenesis during development is emphasised and illustrated using a whole mouse embryo culture system. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10456-008-9105-5Authors Takashi Nagase, National Hospital Organisation Murayama Medical Centre Clinical Research Centre Musashimurayam-shi Tokyo 208–0011 JapanMiki Nagase, University of Tokyo Graduate School of Medicine Department of Nephrology and Endocrinology 7-3-1 Hongo Bunkyo-ku, Tokyo 113-8655 JapanMasafumi Machida, National Hospital Organisation Murayama Medical Centre Clinical Research Centre Musashimurayam-shi Tokyo 208–0011 JapanToshiro Fuji... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1265076 Wed, 27 Feb 2008 15:56:11 +0100 1265076 http://www.medworm.com/index.php?rid=1258045&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fpur514283w75up8r%2F Abstract  The vascular system has the critical function of supplying tissues with nutrients and clearing waste products. To accomplish these goals, the vasculature must be sufficiently permeable to allow the free, bidirectional passage of small molecules and gases and, to a lesser extent, of plasma proteins. Physiologists and many vascular biologists differ as to the definition of vascular permeability and the proper methodology for its measurement. We review these conflicting views, finding that both provide useful but complementary information. Vascular permeability by any measure is dramatically increased in acute and chronic inflammation, cancer, and wound healing. This hyperpermeability is mediated by acute or chronic exposure to vascular permeabilizing agents, particu... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1258045 Fri, 22 Feb 2008 15:47:39 +0100 1258045 http://www.medworm.com/index.php?rid=1246556&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj64298670681r017%2F Abstract  Endoglin is a transmembrane auxillary receptor for transforming growth factor-β (TGF-β) that is predominantly expressed on proliferating endothelial cells. Endoglin deficient mice die during midgestation due to cardiovascular defects. Mutations in endoglin and activin receptor-like kinase 1 (ALK1), an endothelial specific TGF-β type I receptor, have been linked to hereditary hemorrhagic telangiectasia (HHT), an autosomal dominant vascular dysplasia characterized by telangiectases and arteriovenous malformations. Endoglin heterozygote mice develop HHT-like vascular abnormalities, have impaired tumor and post-ischemic angiogenesis and demonstrate an endothelial nitric oxide synthase-dependent deterioration in the regulation of vascular tone. In pre-eclampsia, pla... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1246556 Tue, 19 Feb 2008 15:45:08 +0100 1246556 http://www.medworm.com/index.php?rid=1246555&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb16g218546265107%2F This article reviews the latest information of NRP structure and how structure influences angiogenesis. In addition, the role of NRPs in human cancer is addressed. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s10456-008-9097-1Authors Elena Geretti, Children’s Hospital and Harvard Medical School Department of Surgery, Vascular Biology Program Karp Building 12210, 300 Longwood Ave. Boston MA 02115 USAAkio Shimizu, Children’s Hospital and Harvard Medical School Department of Surgery, Vascular Biology Program Karp Building 12210, 300 Longwood Ave. Boston MA 02115 USAMichael Klagsbrun, Children’s Hospital and Harvard Medical School Department of Surgery, Vascular Biology Program Karp Building 12210, 300 Longwood Ave. Boston MA 02115 USA Journal AngiogenesisO... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1246555 Tue, 19 Feb 2008 15:45:08 +0100 1246555 http://www.medworm.com/index.php?rid=1246554&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F317007063822n441%2F Abstract  Angiogenesis is considered a promising target in the treatment of cancer. Most of the angiogenesis inhibitors in late-stage clinical testing or approved for the treatment of cancer act indirectly on endothelial cells. They either neutralize angiogenic growth factors from the circulation or block the signaling pathways activated by these growth factors. Another group of angiogenesis inhibitors are the direct angiostatic compounds. These agents have a direct effect on the endothelium, affecting cellular regulatory pathways, independently of the tumor cells. The reason that this category of agents is lagging behind regarding their translation to the clinic may be the lack of sufficient knowledge on the mechanism of action of these compounds. The transcription factor ... Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1246554 Tue, 19 Feb 2008 15:45:08 +0100 1246554 http://www.medworm.com/index.php?rid=1236871&cid=s_35902_67_f&fid=35902&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk167632ju50266p7%2F Content Type Journal ArticleCategory Editorial notesDOI 10.1007/s10456-008-9104-6Authors Roy Bicknell, Institute of Biomedical Research, University of Birmingham Centre for Cardiovascular Sciences Wolfson Drive Edgbaston Birmingham B15 2TT UKJudy R. van Beijnum, Research Institute for Growth and Development (GROW) Maastricht University Hospital Angiogenesis Laboratory, Department of Pathology PO Box 5800 6202AZ Maastricht The Netherlands Journal AngiogenesisOnline ISSN 1573-7209Print ISSN 0969-6970 (Source: Angiogenesis) Angiogenesis journals http://www.medworm.com/rss/comments.php?id=1236871 Fri, 15 Feb 2008 18:05:59 +0100 1236871