MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Annals of Clinical and Laboratory Science' source. Sun, 21 Mar 2010 17:30:10 +0100 http://www.medworm.com/index.php?rid=3238489&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124323%26dopt%3DAbstract Authors: Pandolfi F, Cianci R, Cammarota G, Pagliari D, Landolfi R, Conti P, Theoharides TC In recent years, the field of primary immunodeficienciency diseases (PID) has experienced remarkable progress with the identification of a number of new genes associated with specific diseases. Yet the diagnosis of PID remains difficult. In fact, this field requires continuous updating because once a novel molecule related to the immune function is discovered, the corresponding PID will soon be described. Since comprehensive reviews on the classification of PID are available, we concentrate here on reviewing some controversial and new issues, mainly those related to the role of T-cells and innate immunity. We will consider common variable immunodeficiency as an example of a PID where several imm... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238489 Thu, 04 Feb 2010 15:58:03 +0100 3238489 http://www.medworm.com/index.php?rid=3238488&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124324%26dopt%3DAbstract In conclusion, serum homocysteine and Lp(a) concentrations are often increased in FMF patients during attack-free periods. The elevated homocysteine and Lp(a) levels, which are markers of sub-clinical inflammation, may be mediators of atherosclerotic disease in FMF patients. PMID: 20124324 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238488 Thu, 04 Feb 2010 15:58:03 +0100 3238488 http://www.medworm.com/index.php?rid=3238487&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124325%26dopt%3DAbstract Authors: Park HD, Park HK, Chung HS, Lee SY, Kim JW, Ki CS Wilson disease (WD) is an autosomal recessive disorder caused by mutations in the ATP7B gene, yet many patients have either one mutation, or no mutation. We investigated whether the mutation detection rate is associated with any biochemical characteristics of WD. In a study of 71 patients, we used PCR-sequencing to screen for ATP7B mutations in 7 exons (exons 8, 10, 11, 14, 15, 16, and 18) covering 95% of known mutations in Korean patients with WD. We also investigated serum concentrations of various biochemical analytes. Data were analyzed by linear association test and one-way ANOVA. Based on the number of detected ATP7B mutations, a significant difference in serum ceruloplasmin concentration was found among the 3 groups (p &... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238487 Thu, 04 Feb 2010 15:58:03 +0100 3238487 http://www.medworm.com/index.php?rid=3238486&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124326%26dopt%3DAbstract Authors: Park HD, Ki CS, Kim JW, Kim WT, Kim JK Cornelia de Lange syndrome (CdLS; OMIM #122470) is a multiple congenital anomaly with characteristic facial features, growth delay, mental retardation, limb defects, behavioral problems, ocular and hearing impairments, and gastrointestinal or cardiac abnormalities. Although the NIPBL gene has been identified as a causative gene for CdLS, there has hitherto been no genetically confirmed case of CdLS in Korea. Herein, we report a clinical and genetic analysis of three Korean patients with clinical features consistent with CdLS. A male neonate had distinctive facial features, malformations of the upper extremities, genital abnormalities, and bilateral hearing loss, while a 6-yr-old boy and a 10-yr-old girl had distinctive facial features, sh... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238486 Thu, 04 Feb 2010 15:58:03 +0100 3238486 http://www.medworm.com/index.php?rid=3238485&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124327%26dopt%3DAbstract Authors: Rice KM, Walker EM, Kakarla SK, Paturi S, Wu M, Narula S, Blough ER The factors that regulate vascular mechanotransduction and how this process may be altered with aging are poorly understood and have not been widely studied. Recent data suggest that increased tissue loading can result in the release of prostaglandin F2 alpha (PGF2alpha) and other reports indicate that aging diminishes the ability of the aged aorta to activate mitogen activated protein kinase (MAPK) signaling in response to increased loading. Using ex vivo incubations, here we investigate whether aging affects the ability of the aorta to induce phosphorylation of extracellular signal-regulated kinase 1/2 (ERK(1/2)-MAPK), p38-MAPK, and Jun N-terminal kinase (JNK-MAPK) activation following stimulation with a PGF... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238485 Thu, 04 Feb 2010 15:58:03 +0100 3238485 http://www.medworm.com/index.php?rid=3238484&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124328%26dopt%3DAbstract In conclusion, simvastatin administration attenuates cerebral vasospasm and alleviates microthrombosis in the late phase of SAH in this prechiasmatic blood injection model. PMID: 20124328 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238484 Thu, 04 Feb 2010 15:58:03 +0100 3238484 http://www.medworm.com/index.php?rid=3238483&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124329%26dopt%3DAbstract This study was performed to determine the mechanisms for acquiring carbapenem resistance in six clinical isolates of Acinetobacter baumannii. All isolates showed similar SmaI-macrorestriction patterns with less than 3 band differences by PFGE. The isolates showed a high level resistance (>32 mg/L) to both imipenem and meropenem by Etest. Phe-Arg-beta-naphthylamide lowered the MICs of carbapenems. Real-time PCR experiments showed that expression levels of the adeB gene in the six A. baumannii isolates were 10- to 40-times higher than those of imipenem-susceptible strains. Direct sequencing of PCR products showed that all isolates carried the bla(OXA-23) gene, which was preceded by ISAba1. The bla(OXA-23) probe hybridized with approximately 500-kb I-CeuI chromosomal fragments, but not wit... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238483 Thu, 04 Feb 2010 15:58:03 +0100 3238483 http://www.medworm.com/index.php?rid=3238482&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124330%26dopt%3DAbstract This study compared the assay results of HBV markers obtained with two automated immunoassay systems in serum samples with concomitant HBV antigens and antibodies. Eighty-nine samples of concomitant HBsAg and anti-HBs and 74 samples of concomitant HBeAg and anti-HBe were analyzed by the Abbott Architect i2000 and the Roche Elecsys 2010 immunoassay systems. The overall concordance rates of the Architect and Elecsys results were 78.6% for samples with concomitant HBsAg and anti-HBs and 77% for samples with concomitant HBeAg and anti-HBe. The data show that substantial differences exist between the results obtained by these two systems, especially in determining anti-HBs and HBeAg at low concentrations. PMID: 20124330 [PubMed - in process] (Source: Annals of Clinical and Laboratory Scienc... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238482 Thu, 04 Feb 2010 15:58:03 +0100 3238482 http://www.medworm.com/index.php?rid=3238481&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124331%26dopt%3DAbstract Authors: Park J, Chun S, Kim JQ, Song J, Kim JJ, Min WK It is recommended that clinical laboratories keep the bias of serum total cholesterol analysis at </=3.0% compared to a reference method. In Korea, national cholesterol proficiency testing has long been available, but there has been little information about the magnitude of analytical bias. The authors calculated the bias of the peer group mean for Korea's national cholesterol proficiency test through an indirect approach that overcomes the potential matrix effect of proficiency test materials. One laboratory was selected among the proficiency test participants to represent Korean laboratories. Total cholesterol levels of six fresh serums spanning a wide range of concentrations were measured by the representative laboratory and... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238481 Thu, 04 Feb 2010 15:58:03 +0100 3238481 http://www.medworm.com/index.php?rid=3238480&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124332%26dopt%3DAbstract This study examined the effect of storage temperature on the protein profile of human serum. Serum samples were stored for >/=7 days at -80 degrees C, -20 degrees C, 4 degrees C, and room temperature prior to proteomic analysis. Serum protein fractionations by SDS-PAGE, including high and low molecular weight (MW) proteins, showed that several bands had different intensities after storage at higher temperatures. Fractionations by 2-dimensional gel electrophoresis (2-DE) indicated that approximately 60 protein spots had changed significantly after storage at higher temperatures. These proteins included C3/C4, alpha2-macroglobulin, and alpha1B glycoprotein, based on identification by MS and MS/MS. The profile of the low MW serum proteins, analyzed using SELDI ProteinChip Arrays (IMAC3 and... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238480 Thu, 04 Feb 2010 15:58:03 +0100 3238480 http://www.medworm.com/index.php?rid=3238479&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124333%26dopt%3DAbstract Authors: Moody P, Bui MM, Vrionis F, Setzer M, Rojiani AM Primary synovial chondromatosis is an uncommon, benign entity involving the synovial lining of larger joints. In this metaplastic process, islands of chondrocytes are clustered throughout the synovium, forming nodules that jut into the joint cavity. Clinical manifestations include pain, swelling, and decreased range of motion of associated extremities. Synovial chondromatosis may be confused with other degenerative joint diseases as well as chondrosarcoma. The occurrence of synovial chondromatosis in the spine is rare. Ten cases of this spinal variant have previously been published and this report represents the eleventh case. Here we briefly review the literature and discuss the histopathologic pitfalls and differential diagnos... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238479 Thu, 04 Feb 2010 15:58:03 +0100 3238479 http://www.medworm.com/index.php?rid=3238478&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124334%26dopt%3DAbstract We report a testicular lymphoma consistent with diffuse large B-cell lymphoma (DLBCL) in an AIDS patient and we review the literature on primary testicular lymphoma in AIDS patients. PMID: 20124334 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238478 Thu, 04 Feb 2010 15:58:03 +0100 3238478 http://www.medworm.com/index.php?rid=3238477&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124335%26dopt%3DAbstract Authors: Jang JH, Yoo EH, Kim HJ, Kim DH, Jung CW, Kim SH A 57-yr-old woman was diagnosed with acute myeloid leukemia (AML) with maturation, based on morphological and cytochemical/immunophenotypic findings on bone marrow studies. Conventional cytogenetic analysis using bone marrow cells revealed terminal deletion of the short arm of an X chromosome as 46,X,del(X)(p21)[8]/46,XX[12]. On the other hand, fluorescence in situ hybridization (FISH) for the RUNX1/RUNX1T1 (formerly AML1/ETO) rearrangement revealed 86% interphase nuclei with one fusion signal, which was found to be on the long arm of chromosome 8 on metaphase FISH, indicating the RUNX1/RUNX1T1 rearrangement by cryptic insertion of the RUNX1 gene. Molecular genetic study by reverse transcriptase polymerase chain reaction (RT-PCR... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238477 Thu, 04 Feb 2010 15:58:03 +0100 3238477 http://www.medworm.com/index.php?rid=3238476&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124336%26dopt%3DAbstract Authors: Picarelli A, Libanori V, De Nitto D, Saponara A, Di Tola M, Donato G Anti-endomysial and anti-transglutaminase antibodies can be produced in vitro by the intestinal mucosa of celiac disease (CD) patients in clinical remission, when the culture is performed in the presence of gliadin peptides. Our aim was to use this organ culture system as a means to detect the pathognomonic antibodies of celiac disease (CD) in the culture supernatants. Organ culture was performed in the presence of three different activators to evaluate which one induced the strongest antibody response in intestinal mucosa from patients in clinical remission of CD. Our data confirm the high efficiency of synthetic peptide 31-43 as a specific immunological activator in CD and demonstrate its capability to stim... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238476 Thu, 04 Feb 2010 15:58:03 +0100 3238476 http://www.medworm.com/index.php?rid=3238475&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20124337%26dopt%3DAbstract Authors: Hajdu SI PMID: 20124337 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=3238475 Thu, 04 Feb 2010 15:58:03 +0100 3238475 http://www.medworm.com/index.php?rid=2955112&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880759%26dopt%3DAbstract Authors: Chai H, Brown RE The concept "field effect in cancer" originated in 1953 from the histopathological observations of Slaughter and colleagues [1] regarding the occurrence of multiple primary oral squamous cell carcinomas and their local recurrences. The development of modern molecular technologies has extended the field effect concept by exploring the molecular abnormalities in tissues that appear histologically normal. To date, such field effect biomarkers have been reported in several sites and organs, eg, head and neck, colon and rectum, prostate, breast, lung, esophagus, stomach, and skin. Two popular hypotheses have been proposed. One hypothesis implicates genetic alterations that occur in a stepwise fashion (initiation, promotion, and progression); a clone gains growth ad... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955112 Tue, 03 Nov 2009 20:26:02 +0100 2955112 http://www.medworm.com/index.php?rid=2955111&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880760%26dopt%3DAbstract This study shows the dearth of physician blood donors and a strong cohort of institution-affiliated repeat donors. Physicians represent a potential, stable, and sustainable donor pool; further studies are needed to establish physician recruitment programs. PMID: 19880760 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955111 Tue, 03 Nov 2009 20:26:02 +0100 2955111 http://www.medworm.com/index.php?rid=2955110&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880761%26dopt%3DAbstract Authors: Malysz J, Crisan D Activating JAK2 V617F mutation is present in many patients with chronic myeloproliferative neoplasms. We evaluated, retrospectively, clinical and laboratory data from 70 patients with BCR-ABL1 negative, JAK2 positive, chronic myeloproliferative disease. Quantity of the JAK2 mutant allele was tested for correlation with the clinical presentation, type of chronic myeloproliferative disease, hemoglobin level, white blood cell and platelet counts, spleen size, and/or cardiovascular complications. RealTime-PCR was used for amplification of DNA from marrow or peripheral blood. Polycythemia vera was more frequently diagnosed among patients with >or=50% mutational load than among those with <50% mutational load (71% vs 25%; p = 0.003). Patients with >or=50%... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955110 Tue, 03 Nov 2009 20:26:02 +0100 2955110 http://www.medworm.com/index.php?rid=2955109&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880762%26dopt%3DAbstract Authors: Deng L, Boujdir M, Tremontano A, Pincus MR, El-Sherif N PNC-2 is a peptide corresponding to an effector domain (residues 96-110) of ras-p21 that strongly and specifically blocks mitogenic signal transduction by oncogenic but not activated, normally-expressed wild-type ras-p21 protein. Since myocardial hypertrophy can be induced both by oncogenic and overexpressed wild-type ras-p21, we investigated whether PNC-2 can block norepinephrine (NE)-induced, ras-dependent myocardial hypertrophy in cardiac myocytes. Since PNC-2 blocks oncogenic ras-p21-induced activation of JNK and ERK, we further determined whether this peptide blocks activation of these kinases in NE-treated myocytes. Using cultured neonatal rat ventricular myocytes (NRVM), we found that NE alone significantly increas... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955109 Tue, 03 Nov 2009 20:26:02 +0100 2955109 http://www.medworm.com/index.php?rid=2955108&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880763%26dopt%3DAbstract Authors: Lee YK, Kim HS, Kang HJ Transcobalamin-bound vitamin B(12), or holotranscobalamin (HoloTC), a biologically active form, is believed to be a sensitive marker of vitamin B(12) deficiency. We investigated the prevalence of vitamin B(12) deficiency in gastrectomized patients using HoloTC and total vitamin B(12) to determine the diagnostic utility of HoloTC. We enrolled 128 gastrectomized patients and measured serum HoloTC, total vitamin B(12), homocysteine (Hcy), and complete blood count (CBC). HoloTC values were also obtained from 100 healthy controls. The precision of HoloTC measurement was good and the normal range of HoloTC was set at >or=42.48 pmol/L. Among the 128 gastrectomized subjects, HoloTC was low (<42.48 pmol/L) in 32 patients (25.0%) and total vitamin B(12) was... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955108 Tue, 03 Nov 2009 20:26:02 +0100 2955108 http://www.medworm.com/index.php?rid=2955107&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880764%26dopt%3DAbstract Authors: Zappacosta R, Caraceni D, Stura S, Zappacosta B, Rosini S Although gastrointestinal stromal tumors (GISTs) are uncommon, they represent the most frequent mesenchymal neoplasms of the gastrointestinal tract. During recent years, considerable information has been published about the pathogenesis, molecular biology, histological criteria, surgery, and adjuvant pharmacological treatment of GISTs, but there have been few reports about the cytologic diagnosis of GISTs, particularly in effusions; in such specimens these neoplasms cause a wide range of potential pitfalls. In this case report, we show that by combining morphological and immunocytochemical studies on thin layer slide preparations, the cytologic diagnosis of GISTs can be both accurate and efficient. PMID: 19880764 [P... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955107 Tue, 03 Nov 2009 20:26:02 +0100 2955107 http://www.medworm.com/index.php?rid=2955106&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880765%26dopt%3DAbstract Authors: Chandrasena LG, Peiris H, Waikar HD A prospective study was performed to monitor the postoperative changes in biochemical markers associated with reperfusion injury following (i) cardiopulmonary bypass (CPB) with aortic cross-clamping and cardioplagia (CABG); (ii) CPB with a tissue stabilizing device (SUP.CPB); or (iii) surgery on beating heart (off-pump CABG or OPCABG). Of the 48 patients, 16 were subjected to CABG, 16 to SUP.CPB, and 16 to OPCABG. Arterial and venous blood samples drawn 10 min preoperatively and 0.2, 4, 24, and 48 hr after surgery were assayed for plasma lactate, total calcium, and ionized calcium and erythrocyte glutathione peroxidase (GPX) and superoxide dismutase (SOD). Results revealed that ionized calcium, SOD, and GPX levels of all patients increased a... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955106 Tue, 03 Nov 2009 20:26:02 +0100 2955106 http://www.medworm.com/index.php?rid=2955105&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880766%26dopt%3DAbstract This study tested if acetaminophen, N-methyl-D-glucamine dithiocarbamate (NMGDTC), deferoxamine, and combinations of these agents reduce excess iron content, prevent iron-induced pathology, reduce cardiac arrhythmias, and reduce mortality in iron-overloaded gerbils. Eight groups of 16 gerbils received iron dextran injections (ferric hydroxide dextran complex, 120 mg/kg, ip) or saline solution (controls) twice/wk for 8 wk. The 8 groups were treated every Monday, Wednesday, and Friday with one of the following: saline control, acetaminophen, 150 mg/kg, ip), acetaminophen (150 mg/kg, po), deferoxamine, 83 mg/kg, ip), NMGDTC (200 mg/kg, ip), or combinations of acetaminophen (75 mg/kg) with deferoxamine (42 mg/kg, each ip, separately) or acetaminophen (75 mg/kg) with NMGDTC (100 mg/kg, each ip,... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955105 Tue, 03 Nov 2009 20:26:02 +0100 2955105 http://www.medworm.com/index.php?rid=2955104&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880767%26dopt%3DAbstract This study investigated in rabbits whether a high methionine (HM) diet influences oxidative stress parameters in serum, apo-B containing lipoproteins (LDL+VLDL), heart, and aorta. Rabbits received a normal commercial chow supplemented with 2% L-methionine (w/w) for 6 mo (approximately 1 g/kg body wt/day). Serum homocysteine (HCys), malondialdehyde (MDA), diene conjugate (DC), and cholesterol levels were found to be increased, but protein carbonyl (PC) and triglyceride levels remained unchanged in the HM group as compared to controls. Cholesterol, endogenous DC, and copper-induced MDA levels were significantly higher in the LDL+VLDL fraction of plasma lipoproteins in the HM group. MDA and DC levels were found to be increased in homogenates of heart and aorta in the HM group. The HM diet cau... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955104 Tue, 03 Nov 2009 20:26:02 +0100 2955104 http://www.medworm.com/index.php?rid=2955103&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880768%26dopt%3DAbstract Authors: Kim SJ, Park TS, Lee ST, Song J, Suh B, Kim SH, Jang SJ, Lee CH, Choi JR Therapy-related myelodysplastic syndrome and acute leukemia after treatment with temozolomide have rarely been described in the literature. Only 10 cases in association with temozolomide have been documented. The cases included anaplastic astrocytoma (4 cases), anaplastic oligodendroglioma (2 cases), low grade astrocytoma (2 cases), low grade oligodendroglioma (1 case), and one case of secondary Philadelphia-positive acute lymphoblastic leukemia in a patient with glioblastoma multiforme. Here we report a novel case of therapy-related myelodysplastic syndrome/acute myeloid leukemia associated with der(1;7)(q10;p10) in a glioblastoma multiforme patient treated with temozolomide. Results of bone marrow morph... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955103 Tue, 03 Nov 2009 20:26:02 +0100 2955103 http://www.medworm.com/index.php?rid=2955102&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880769%26dopt%3DAbstract Authors: Park HD, Kim SR, Ki CS, Lee SY, Chang YS, Jin DK, Park WS Mitochondrial trifunctional protein (MTP) is a heterocomplex composed of 4 alpha-subunits containing LCEH (long-chain 2,3-enoyl-CoA hydratase) and LCHAD (long-chain 3-hydroxyacyl CoA dehydrogenase) activity, and 4 beta-subunits that harbor LCKT (long-chain 3-ketoacyl-CoA thiolase) activity. MTP deficiency is an autosomal recessive disorder that causes a clinical spectrum of diseases ranging from severe infantile cardiomyopathy to mild chronic progressive polyneuropathy. Here, we report the case of a Korean male newborn who presented with severe lactic acidosis, seizures, and heart failure. A newborn screening test and plasma acylcarnitine profile analysis by tandem mass spectrometry showed an increase of 3-hydroxy speci... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955102 Tue, 03 Nov 2009 20:26:02 +0100 2955102 http://www.medworm.com/index.php?rid=2955101&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880770%26dopt%3DAbstract Authors: de Pádua RA, de Lima Scodro RB, Ghiraldi LD, Siqueira VL, Yamashita YK, Helbel C, Cardoso RF This work describes a case of Haemophilus influenzae serotype a meningitis in Brazil, after almost a decade since the introduction of Haemophilus influenzae serotype b conjugate vaccine. Uncertainty about the replacement of H. influenzae serotypes as a cause of invasive diseases justifies continuous surveillance, coupled with investigations of carriage rates and requirements of chemoprophylaxis in contact persons. PMID: 19880770 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955101 Tue, 03 Nov 2009 20:26:02 +0100 2955101 http://www.medworm.com/index.php?rid=2955100&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880771%26dopt%3DAbstract We report the identification of Histoplasma capsulatum var. capsulatum from whole blood in a renal transplant patient with disseminated histoplasmosis using colorimetric microtiter-plate PCR. This modality demonstrated utility in reaching a definitive diagnosis in a timely manner. Blood fungal cultures in this case remained negative, suggesting that molecular assays may facilitate the laboratory diagnosis of disseminated histoplasmosis. PMID: 19880771 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955100 Tue, 03 Nov 2009 20:26:02 +0100 2955100 http://www.medworm.com/index.php?rid=2955099&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19880772%26dopt%3DAbstract Authors: Hajdu SI, Tang P PMID: 19880772 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2955099 Tue, 03 Nov 2009 20:26:02 +0100 2955099 http://www.medworm.com/index.php?rid=2692234&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667406%26dopt%3DAbstract Authors: McCully KS This review considers recent advances in the chemical pathology of homocysteine in atherogenesis, oxidative metabolism, and carcinogenesis. Homocysteine is a potent excitatory neurotransmitter that binds to the N-methyl-D-aspartate (NMDA) receptor and leads to oxidative stress, cytoplasmic calcium influx, cellular apoptosis, and endothelial dysfunction. According to the adsorption-induction theory, cytoplasmic calcium influx leads to depletion of cellular adenosine triphosphate (ATP) by reaction with cytoplasmic phosphate, leading to calcium apatite deposition. Oxidative stress is caused by failure of ATP synthesis and accumulation of reactive oxygen radicals, theoretically because of inhibition of thioretinaco ozonide function within mitochondria and endoplasmic re... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692234 Wed, 12 Aug 2009 19:02:03 +0100 2692234 http://www.medworm.com/index.php?rid=2692233&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667407%26dopt%3DAbstract This study was conducted to evaluate the analytical performance of a new colorimetric assay for plasma homocysteine (Hcy) and to determine the influence of the method used on the frequency of hyperhomocysteinemia and on the association between Hcy and biological parameters in diabetic patients. Plasma Hcy was measured in 160 diabetic patients by high performance liquid chromatography (HPLC) in parallel with an automated colorimetric assay (DiaSys Diagnostic Systems GmbH) using the Olympus AU640 and Abbott C8000 analyzers. Plasma glucose, creatinine, folates, vitamin B(12), high-sensitivity C-reactive protein (hs-CRP), and blood hemoglobin A1c (HbA1c) concentrations were measured by routine methods. The Hcy concentrations obtained with the HPLC (x) and colorimetric methods (y) were highly c... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692233 Wed, 12 Aug 2009 19:02:03 +0100 2692233 http://www.medworm.com/index.php?rid=2692232&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667408%26dopt%3DAbstract Authors: Lee JY, Prineas RJ, Eaton JW Individuals genetically susceptible to hypertension may have preexisting membrane defects influencing cell sodium permeability. Fourteen Minnesotan families of Northern European descent were selected as having one or both progenitors with either high (HP) or low (LP) erythrocyte sodium permeability. We earlier found that over one-half of the (22)Na(+) influx into HP erythrocytes can be inhibited by micromolar amounts of furosemide, which has no apparent effect on LP erythrocytes. In these families, we find a significant midpoint parent/offspring correlation in the furosemide-sensitive component of erythrocyte (22)Na(+) flux rates (p <0.001). The relationship between parents and children in this metric trait is most consistent with a single locus... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692232 Wed, 12 Aug 2009 19:02:03 +0100 2692232 http://www.medworm.com/index.php?rid=2692231&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667409%26dopt%3DAbstract Authors: Karaczyn AA, Cheng RY, Buzard GS, Hartley J, Esposito D, Kasprzak KS Nickel(II), capable of transforming cells and causing tumors in humans and animals, has been previously shown by us to mediate hydrolytic truncation of histone H2A's C-terminal tail by 8 amino acids in both cell-free and cell culture systems. Since H2A's C-tail is involved in maintaining chromatin structure, such truncation might alter this structure and affect gene expression. To test the latter possibility, we transfected cultured T-REx 293 human embryonic kidney cells with plasmids expressing either wild type (wt) or truncated (q) histone H2A proteins, which were either untagged or N-terminally tagged with fluorescent proteins. Each histone variant was found to be incorporated into chromatin at 24 and 48 h... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692231 Wed, 12 Aug 2009 19:02:03 +0100 2692231 http://www.medworm.com/index.php?rid=2692230&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667410%26dopt%3DAbstract Authors: Wu PP, Jin YL, Shang YF, Jin Z, Wu P, Huang PL DLC1 (deleted in liver cancer-1) is a new candidate tumor suppressor gene, which is inactive in various types of human cancers including colon cancer. To study the function of DLC1, we constructed a pcDNA3.1 vector containing the DLC1 gene and transfected it into HT29 colon cancer cells that were deficient in DLC1 expression. The restoration of DLC1 expression in HT29 cells significantly inhibited cell proliferation and migration. Flow cytometry showed that DLC1 transfection into HT29 cells induced apoptosis and that the cell cycle was arrested at S-phase. Additionally, cyclinD1 mRNA and protein expression were down-regulated while p21 expression was increased in pcDNA3.1-DLC1-HT29 cells compared to wild HT29 cells. These results ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692230 Wed, 12 Aug 2009 19:02:03 +0100 2692230 http://www.medworm.com/index.php?rid=2692229&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667411%26dopt%3DAbstract This study analyzes the interobserver variability in interpreting atypia on breast core needle biopsies and in each category of atypia calculates the upgrade risk of carcinoma in the subsequent surgical excision. We identified 51 cases of atypia on breast core needle biopsies performed at our institution from January 2003 to August 2006. The atypia was classified into 4 categories: atypical ductal hyperplasia (ADH), atypical lobular hyperplasia (ALH), flat epithelial atypia (FEA), and atypia of undetermined significance (AUS). After a tutorial session, these cases were independently reviewed by four pathologists, whose overall multi-rater kappa value for agreement on different categories of atypia was 0.79 (95% CI, 0.69-0.89), which is within the substantial agreement range. The upgrade ri... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692229 Wed, 12 Aug 2009 19:02:03 +0100 2692229 http://www.medworm.com/index.php?rid=2692228&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667412%26dopt%3DAbstract We report 5 cases of adrenocortical neoplasms in young children and review their clinical presentations, pathology, and follow-up data. Pathologic evaluations included histology and immunostains with p53 and Ki-67. The patients were 3 girls and 2 boys, 13-28 mo of age. The patients all presented with virilization and other hormone-related symptoms for an average duration of 6 mo. Serum testosterone levels were elevated in 3 cases. Imaging studies revealed neoplasms in the left adrenal gland in 3 cases and the right adrenal gland in 2 cases. No evidence of disease was identified at any other site of the body. The tumors were grossly confined to the adrenal glands and ranged in diameter from 3 to 6 cm (mean 4.3 cm). Microscopically, the tumors had histological and immunophenotypic features c... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692228 Wed, 12 Aug 2009 19:02:03 +0100 2692228 http://www.medworm.com/index.php?rid=2692227&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667413%26dopt%3DAbstract This study demonstrates that flow cytometric detection of PAIg combined with ROC curve analysis is a convenient, sensitive, and specific test, compared to previous methods, and it is useful for the differential diagnosis of thrombocytopenic patients. PMID: 19667413 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692227 Wed, 12 Aug 2009 19:02:03 +0100 2692227 http://www.medworm.com/index.php?rid=2692226&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667414%26dopt%3DAbstract The objective of this study was to identify possible risk factors associated with a lack of response to aspirin and clopidogrel treatments in patients with coronary or cerebral ischemic artery disease. A point-of-care analyzer, VerifyNow (Accumetrics, San Diego, CA), was used to measure adenosine-5-diphosphate and platelet P2YI2 receptor blockage to investigate the responses of a group of 197 patients to aspirin and/ or clopidogrel therapies (aspirin therapy, 178; clopidogrel therapy, 139; both drugs, 144). Of these 197 patients, 135 (68.5%) had coronary artery disease and 72 (31.5%) had ischemic cerebrovascular disease. Aspirin resistance was defined as an ARU (aspirin reaction units) > or =550, and clopidogrel resistance was defined as platelet inhibition <20%. Twenty-five of 178 a... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692226 Wed, 12 Aug 2009 19:02:03 +0100 2692226 http://www.medworm.com/index.php?rid=2692225&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667415%26dopt%3DAbstract We report a case of a primitive neuroectodermal tumor (PNET) arising in the cerebellum and pons 7 yr after intracranial radiation and chemotherapy for leukemia involving the CNS. This case suggests a possible link between radiation, chemotherapy, and the formation of these tumors, with a potential new pathogenetic role for somatic inactivation of the protooncogene RET. PMID: 19667415 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692225 Wed, 12 Aug 2009 19:02:03 +0100 2692225 http://www.medworm.com/index.php?rid=2692224&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667416%26dopt%3DAbstract In this study, we found a Korean family showing clinical features of the disease. Mutation analysis of the EYA1 gene revealed a novel one-base-pair deletion resulting in truncated protein (c.321delT; p.Ala107fs). This is the first report of BOR syndrome caused by deletion mutation of the EYA1 gene in Korea. PMID: 19667416 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692224 Wed, 12 Aug 2009 19:02:03 +0100 2692224 http://www.medworm.com/index.php?rid=2692223&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667417%26dopt%3DAbstract Authors: Suh B, Park TS, Kim JS, Song J, Kim J, Yoo JH, Choi JR The chromosomal abnormality der(9)t(1;9)(q11;q34) is a rare occurrence in patients with hematologic malignancies. As far as we know, only 3 cases of acute myeloid leukemia, 1 case of polycythemia vera, and 1 case of multiple myeloma with this derivative chromosome have been reported in the literature. Here we report the first case of der(9)t(1;9)(q11;q34) in a patient with chronic myelomonocytic leukemia (CMML). A 45-yr-old man was brought to our hospital for evaluation of pancytopenia and monocytosis. The patient's persistent monocytosis in peripheral blood and his bone marrow findings were consistent with the diagnosis of CMML. Chromosome study results repeatedly showed 46,XY,der(9)t(1;9)(q11;q34). In addition, the BCR/A... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692223 Wed, 12 Aug 2009 19:02:03 +0100 2692223 http://www.medworm.com/index.php?rid=2692222&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667418%26dopt%3DAbstract Authors: DeBari VA It has been demonstrated that decision levels (DL) and their confidence intervals (CI) can be estimated from the second derivative, f '' (P), of the logistic regression probability curve (LRPC). Although this method generally provides smooth curves from which DL and CI can be obtained, there are datasets that generate "noisy" curves making these measurements difficult. The purpose of this study was to develop a procedure to obviate this noise, thus allowing the more facile estimation of DL and CI. Data from two clinical studies were examined. Logistic regression analysis was performed and the first derivatives, f ' (P), were fitted to Gaussian models. The derivatives of these surrogate f ' (P) were generated to provide f '' (P) and were compared with data from receiv... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692222 Wed, 12 Aug 2009 19:02:03 +0100 2692222 http://www.medworm.com/index.php?rid=2692221&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19667419%26dopt%3DAbstract Authors: Hajdu SI PMID: 19667419 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2692221 Wed, 12 Aug 2009 19:02:03 +0100 2692221 http://www.medworm.com/index.php?rid=2525204&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429794%26dopt%3DAbstract Authors: Tantibhaedhyangkul U, Tatevian N, Gilger MA, Major AM, Davis CM Eosinophilic esophagitis (EE) and gastroesophageal reflux disease (GERD) have similar clinical presentations. The immunoregulatory mechanisms involved in both diseases are not clearly defined. We studied cellular inflammation in pediatric patients with EE and GERD compared to normal controls (NC). Pathology records were reviewed of 10 EE, 8 GERD, and 10 NC children who were seen at Texas Children's Hospital in the past 3 yr. FOXP3, CD4, CD8, CD25, eotaxin-3, and IL-5 immunohistochemical stains were performed on formalin-fixed, paraffin-embedded esophageal tissue sections and assessed by a blinded observer. The numbers of FOXP3(+), CD25(+), and CD8(+) cells were significantly increased in both EE and GERD compared ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525204 Sat, 27 Jun 2009 11:00:09 +0100 2525204 http://www.medworm.com/index.php?rid=2525202&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429795%26dopt%3DAbstract In conclusion, this pilot study suggests that CD5 staining may be used to differentiate benign muscle tissue from malignancy. Albeit our case series is limited, this study indicates potential utility of CD5 staining in diagnosis of muscle tumors. PMID: 19429795 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525202 Sat, 27 Jun 2009 11:00:09 +0100 2525202 http://www.medworm.com/index.php?rid=2525201&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429796%26dopt%3DAbstract In conclusion, measurement of FAD fluorescence intensity in neutrophils by flow cytometry after incubation with bis(Zn(2+)-dipicolylamine) complex is an easy, objective, and reliable method of detecting neutrophils with toxic granulation. PMID: 19429796 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525201 Sat, 27 Jun 2009 11:00:09 +0100 2525201 http://www.medworm.com/index.php?rid=2525199&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429797%26dopt%3DAbstract Authors: Kolovou G, Damaskos D, Anagnostopoulou K, Cokkinos DV Many studies have shown that the prevalence and onset of coronary heart disease (CHD) is sex-dependent. CHD prevalence is lower in women than in men at all ages. Furthermore, women's age of CHD onset seems to be 10 yr later. This is widely attributed to the fact that men have less favorable CHD risk factors (eg, plasma lipid profile) compared to women. Mean levels of protective high density lipoprotein cholesterol are lower, while triglyceride levels are higher in men than in women. It is possible that many of the genes involved in lipid metabolism, such as Apolipoprotein (Apo) E, as well as their polymorphisms, may be expressed in a sexually dimorphic manner. The human Apo E gene is polymorphic, encoding one of 3 common ep... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525199 Sat, 27 Jun 2009 11:00:09 +0100 2525199 http://www.medworm.com/index.php?rid=2525197&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429798%26dopt%3DAbstract This study utilized a radiolabeled monoclonal antibody specific to human apolipoprotein E fragments and human apolipoprotein E-knock-in mice, in which AA amyloidosis was induced. Accumulation of the injected radiolabeled antibody was significantly higher in the organs of amyloidotic mice than in those of non-amyloidotic mice. Plasma clearance of the antibody did not differ between the amyloidotic and non-amyloidotic mice. The antibody was given to mice during amyloid induction but failed to prevent amyloidogenesis. The results of this initial study are encouraging, but considerable improvement is necessary, particularly in regard to development of a high affinity antibody. PMID: 19429798 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525197 Sat, 27 Jun 2009 11:00:09 +0100 2525197 http://www.medworm.com/index.php?rid=2525195&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429799%26dopt%3DAbstract Authors: Reiss RF, Harkin R, Lessig M, Mascari J Given the paucity of published data regarding reaction rates in younger teenaged donors, we evaluated the reaction rates in all of our first time teenaged donors after New York Blood Center lowered the minimum permissible age for blood donations from 17 to 16 yr in 2005. The overall rates of vaso-vagal reactions in donors aged 16 to 19, and those resulting in syncope, occurring in 72,769 consecutive first time whole blood, 3,822 double red cell, and 777 platelet apheresis donations were calculated. They were correlated with age and compared to those found in donors aged 20-29. Separate rates were calculated by gender, age in yr, and donation type, and then compared to each other. The overall reaction rate among first time teenaged whole ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525195 Sat, 27 Jun 2009 11:00:09 +0100 2525195 http://www.medworm.com/index.php?rid=2525193&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429800%26dopt%3DAbstract Authors: Chung HJ, Lee W, Chun S, Park HI, Min WK The Department of Laboratory Medicine at Asan Medical Center provides a special one-stop service to allow both doctors and outpatients to receive routine outpatient test results in a timely manner within 1 hr. We developed a new Laboratory Information System (LIS)-based monitoring system that records the laboratory turnaround time (TAT) in 3 phases and analyzes the time to complete each phase with relevant specimens. TAT is subdivided into preanalytical, analytical, and postanalytical phases based on the 4 time points when data are entered automatically into the LIS. The average TAT for 13,594 outpatient routine chemistry specimens with the one-stop service was 43.6+/-7.7 min. Completion times of the preanalytical, analytical, and posta... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525193 Sat, 27 Jun 2009 11:00:09 +0100 2525193 http://www.medworm.com/index.php?rid=2525191&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429801%26dopt%3DAbstract Authors: Chung HJ, Lee W, Chun S, Kang SY, Lee WI, Park HI, Min WK The quality control (QC) failure rate in the serum total carbon dioxide (TCO(2)) test increases at a higher rate than in other tests over time after calibration. The causes of the increased QC failure rate in the TCO(2) test were examined. Using a TBA200RF analyzer (Toshiba Medical Systems), the TCO(2) of the QC material was measured at 2-hr intervals and was found to decrease by up to 16.5% at 10 hr after calibration. In contrast, using the P-module and D-module analyzers (Roche Diagnostics), the TCO(2) of the QC material did not change significantly during 10 hr after calibration. When the TCO(2) level of the QC material was measured hourly over 5 hr with the TBA200FR analyzer while the reagent bottle was rotated at 0... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525191 Sat, 27 Jun 2009 11:00:09 +0100 2525191 http://www.medworm.com/index.php?rid=2525189&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429802%26dopt%3DAbstract This study describes the validation of the TaqMan Influenza A Detection Kit v2.0 combined with an automated nucleic acid extraction method. The limit of detection of this assay was determined by probit regression (95% confidence interval) to be 2 influenza A/PR/8/34 (H1N1) virus particles per microlitre. One hundred and eleven specimens previously tested using the Seeplex RV assay and viral culture methods were tested using the TaqMan Influenza A Detection Kit. Compared to the aggregate gold-standard, the sensitivity and specificity of the TaqMan Influenza A Detection Kit were 100% (35/35) and 97% (74/76), respectively. Because of its accuracy, quick turn-around-time and lyophilized bead form, the TaqMan Influenza A Detection Kit, combined with the NucliSense easyMAG automated extraction m... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525189 Sat, 27 Jun 2009 11:00:09 +0100 2525189 http://www.medworm.com/index.php?rid=2525187&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429803%26dopt%3DAbstract This study examines the cytogenetic and molecular correlates and provides insight into therapeutic strategies relevant to this morphoproteomic profile. Based on a literature review, these observations appear to be the first morphoproteomic study of such pathways of convergence in tumors from EFT patients. PMID: 19429803 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525187 Sat, 27 Jun 2009 11:00:09 +0100 2525187 http://www.medworm.com/index.php?rid=2525185&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429804%26dopt%3DAbstract Authors: Shakov R, DePasquale JR, Elfarra H, Spira RS Autoimmune pancreatitis (AuP) is a chronic pancreatic inflammation secondary to an underlying autoimmune mechanism. After early reports of a particular type of pancreatitis associated with hypergammaglobulinemia, others asserted that there is an autoimmune mechanism involved in some patients with chronic pancreatitis. In 1995 AuP was first described as a distinct clinical entity. Since then, there have been many documented cases of AuP in Japan, and now, perhaps due to increased awareness, more cases are being reported in Europe and the United States. Herein we present our experience with 3 cases of AuP and we review the relevant literature. These 3 cases demonstrate the difficulties that exist in making the diagnosis of AuP and the... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525185 Sat, 27 Jun 2009 11:00:09 +0100 2525185 http://www.medworm.com/index.php?rid=2525183&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429805%26dopt%3DAbstract We describe a novel case of simultaneous karyotypic abnormalities of isochromosome 17q and trisomy 14 in a patient with myelodysplastic syndrome (MDS) in leukemic transformation. A 66-yr-old Korean man was admitted to Severance Hospital for evaluation of pancytopenia. On the basis of bone marrow studies at 3 different stages, he was diagnosed with MDS in leukemic transformation. Chromosome karyotyping repeatedly showed the same main clonal abnormalities, including isochromosome 17q and trisomy 14. Isochromosome 17q and trisomy 14 have each been reported as rare, nonrandom recurrent chromosomal abnormalities in patients with MDS showing a poor prognosis. To our knowledge, this is the first report of concurrent i(17)(q10) and trisomy 14 in a patient with MDS in leukemic transformation. P... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525183 Sat, 27 Jun 2009 11:00:09 +0100 2525183 http://www.medworm.com/index.php?rid=2525181&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429806%26dopt%3DAbstract Authors: Kim J, Yang KH, Nam JS, Choi JR, Song J, Chang M, Lee KA Mutations including nonsense mutations, missense mutations, splicing-site mutations, insertions, and deletions in phosphate regulating genes on the X-chromosome (PHEX) are known to be responsible for X-linked hypophosphatemic rickets. The PHEX gene encodes an endopeptidase that is involved in phosphate regulation. Herein we present a female patient with sporadic hypophosphatemic rickets harboring a novel deletion mutation (c.1586_1586+1delAG; p.Glu529GlyfsX41) at exon 14 and intron 14 junction, which caused a premature termination at codon 569 and possibly produced a truncated PHEX protein. The laboratory and radiologic findings of the patient are reviewed to correlate the impact of the two-base deletion mutations at the... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525181 Sat, 27 Jun 2009 11:00:09 +0100 2525181 http://www.medworm.com/index.php?rid=2525179&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429807%26dopt%3DAbstract Authors: Park HD, Moon HK, Lee J, Lee M, Lee SY, Kim JW, Ki CS Menkes disease (MD, MIM 309400) is a fatal X-linked recessive disorder that is caused by mutations in the gene encoding ATP7A, a copper-transporting, P-type ATPase. Patients with MD are characterized by progressive hypotonia, seizures, failure to thrive, and death in early childhood. Two Korean patients were diagnosed with Menkes disease by clinical and biochemical findings. We found one missense mutation and one gross deletion in the ATP7A gene in the patients. The missense mutation in Patient 1, c.3943G>A (p.G1315R) in exon 20, was identified in a previous report. Patient 2 had a gross deletion of c.1544-?_2916+?, which was a novel mutation. The patients' mothers were shown to be carriers of the respective mutations. P... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525179 Sat, 27 Jun 2009 11:00:09 +0100 2525179 http://www.medworm.com/index.php?rid=2525177&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429808%26dopt%3DAbstract Authors: Franklin G, Greenspan J, Chen S It has been reported that anti-tumor necrosis factor-alpha therapy increases the risk of opportunistic infections including rare case reports of leishmaniasis. Here we report a case of latent cutaneous leishmaniasis, which was provoked by anti-tumor necrosis factor-alpha therapy in a patient with rheumatoid arthritis. PMID: 19429808 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525177 Sat, 27 Jun 2009 11:00:09 +0100 2525177 http://www.medworm.com/index.php?rid=2525175&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19429809%26dopt%3DAbstract Authors: Hajdu SI PMID: 19429809 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2525175 Sat, 27 Jun 2009 11:00:09 +0100 2525175 http://www.medworm.com/index.php?rid=2174117&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201735%26dopt%3DAbstract Authors: Ravnskov U, McCully KS Little attention has been paid to the function of lipoproteins as part of a nonspecific immune defense system that binds and inactivates microbes and their toxins effectively by complex formation. Because of high extra-capillary tissue pressure, aggregates of such complexes may be trapped in vasa vasorum of the major arteries. This complex formation and aggregation may be enhanced by hyperhomocysteinemia, because homocysteine thiolactone reacts with the free amino groups of apo-B to form homocysteinylated low-density lipoprotein (LDL), which is subject to spontaneous precipitation in vitro. Obstruction of the circulation in vasa vasorum, caused by the aggregated complexes, may result in local ischemia in the arterial wall, intramural cell death, bursting... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174117 Wed, 11 Feb 2009 01:15:01 +0100 2174117 http://www.medworm.com/index.php?rid=2174116&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201736%26dopt%3DAbstract Authors: Lester T, Wang J, Bourne P, Yang Q, Fu L, Tang P Mammary Paget's disease (MPD) is a rare manifestation of breast carcinoma involving the nipple. Our objective was to identify molecular markers and molecular subtypes that may predict patients at high risk of developing MPD. Immunohistochemical (IHC) analyses were performed with antibodies to estrogen receptor (ER), progesterone receptor (PR), androgen receptor (AR), HER2, epidermal growth factor receptor (EGFR), and several cytokeratins (CK5/6, CK14, CK17, CK8, CK18) on representative sections of 121 cases of ductal carcinoma of the breast, including 28 cases with MPD, 81 cases with neither MPD nor nipple involvement, and 12 cases of non-MPD with nipple involvement. The rates of receptor expression and subtype distributions of ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174116 Wed, 11 Feb 2009 01:15:01 +0100 2174116 http://www.medworm.com/index.php?rid=2174115&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201737%26dopt%3DAbstract Authors: Stevens E, Kimler BF, Davis MK, Fan F, Thomas P, Wang XY, Damjanov I, Tawfik OW Invasive ductal carcinoma (IDC) of the breast is currently graded according to the Nottingham modification of the Scarff-Bloom-Richardson system (SBR). This system involves subjective evaluation of 3 morphologic features: tubule formation, nuclear pleomorphism, and mitosis. Our recently proposed semi-automated Nuclear and Proliferation Index [N+P] grading system for IDC has demonstrated agreement among grades and prognostic markers with better prediction of patient survival than the SBR system. Our present objective is to expand the utilization of the N+P system to grading invasive lobular carcinoma (ILC). Fifty-eight ILC cases were evaluated by the SBR and N+P systems. The 2 systems were compared ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174115 Wed, 11 Feb 2009 01:15:01 +0100 2174115 http://www.medworm.com/index.php?rid=2174114&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201738%26dopt%3DAbstract Authors: Edwards HD, Sabio H, Shihabi ZK A rapid ( approximately 90 sec), fully automated method is described for quantifying hemoglobin S (HbS) by high performance liquid chromatography (HPLC) using the Bio-Rad Variant II Turbo analyzer. Although this instrument is designed to quantify only blood hemoglobin A1c (HbA1c), we show that it can also quantify accurately, without modification, HbS levels in sickle cell patients, provided the blood samples meet certain conditions. The samples should contain detectable hemoglobin F (HbF), but should not contain hemoglobin C (HbC). Under these conditions, blood HbS levels obtained by this method correlate well with those obtained by agarose electrophoresis (r(2) = 0.97, n = 81 patients). We also show that quantitation of blood HbF in sickle cel... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174114 Wed, 11 Feb 2009 01:15:01 +0100 2174114 http://www.medworm.com/index.php?rid=2174113&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201739%26dopt%3DAbstract In this study, we performed genetic polymorphism analyses by molecular techniques of the Lutheran blood group system in Chinese subjects. Blood samples were collected from randomly-selected healthy donors and analyzed by PCR-RFLP or SBT methods. LU1/2(Lu(a)/Lu(b)), LU6/9, LU8/14, and LU18/LU19(Au(a)/Au(b)) antigen polymorphisms were detected as follows: 1102 individuals were diagnosed as Lu(a-b+) type; 117 individuals were all LU(6+9-) genotypes; 119 individuals were all LU(8+14-) genotypes. Among 368 individuals, 278 showed homozygous nt1615A, 6 showed homozygous nt1615G, and 84 showed nt1615A/G heterozygosity. The gene frequencies of Au(a) and Au(b) in Chinese subjects were 0.8695 and 0.1304 respectively. A novel allele was identified in 4 Lu(18+19-) phenotype cases from 3 families. ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174113 Wed, 11 Feb 2009 01:15:01 +0100 2174113 http://www.medworm.com/index.php?rid=2174112&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201740%26dopt%3DAbstract Authors: Smith-Norowitz TA, Josekutty J, Lev-Tov H, Kohlhoff S, Norowitz KB, Silverberg JI, Chice S, Durkin HG, Bluth MH Blood lymphocyte distributions, serum immunoglobulin and cytokine levels, and serum IgE and IgG anti-varicella zoster virus (VZV) levels were measured in an atopic girl (age 15 yr) who developed shingles 10 yr after infection with chicken pox. Before, during, and 5 months after the shingles episode, the child's distributions of blood lymphocytes (excluding CD23+) and serum immunoglobulin levels (excluding IgE) were within the normal ranges. Her blood level of CD23+ lymphocytes decreased >50% during the shingles episode and remained low thereafter. Her serum level of IgE was elevated before and during shingles (154 and 168 IU/ml, respectively), but was reduced afte... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174112 Wed, 11 Feb 2009 01:15:01 +0100 2174112 http://www.medworm.com/index.php?rid=2174111&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201741%26dopt%3DAbstract This study shows that 94% of the strains preserved at -70 degrees C on glass beads could be recovered within 30 days. The recovery rates for drug-susceptible and drug-resistant strains showed no significant differences. The growth rates and the number of strains that showed abundant growth before the 30th day of incubation represent important features, since the subculture of a strain preserved for future use ought to quickly produce abundant growth in order to avoid misinterpretation of the tests. Our experience indicates that storage of M. tuberculosis on glass beads at -70 degrees C is a suitable procedure for an active culture collection in a public health laboratory like ours, where maintenance of M. tuberculosis cultures is a complementary activity and must be quick, practical, effec... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174111 Wed, 11 Feb 2009 01:15:01 +0100 2174111 http://www.medworm.com/index.php?rid=2174110&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201742%26dopt%3DAbstract Authors: Park YJ, Yu JK, Kim SI, Lee K, Arakawa Y A new plasmid-mediated fluoroquinolone efflux pump gene, qepA, is known to be associated with the rmtB gene, which confers high-level resistance to aminoglycosides. We investigated the qepA gene in 573 AmpC-producing Enterobacteriaceae including one Citrobacter freundii known to harbor rmtB. Of them, two clonally unrelated E. aerogenes harbored qepA. Both isolates co-harbored rmtB, qnrS1, qepA, and bla(LAP-1) on an IncFI type plasmid. The qepA was flanked by two copies of IS26 containing ISCR3C, tnpA, tnpR, bla(TEM), and rmtB. The qnrS1 and bla(LAP-1) were located upstream of qepA. All the resistance determinants (qepA, qnrS1, rmtB, and bla(LAP-1)) were co-transferred to E. coli J53 by filter mating from both isolates. Although the prev... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174110 Wed, 11 Feb 2009 01:15:01 +0100 2174110 http://www.medworm.com/index.php?rid=2174109&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201743%26dopt%3DAbstract Authors: Sleiman JN, Kohlhoff SA, Roblin PM, Wallner S, Gross R, Hammerschlag MR, Zenilman ME, Bluth MH Sophorolipids (SLs), glycolipids produced by yeasts, have been reported to have immunomodulating activity and to reduce the mortality rate in animal models of sepsis. In the present study, the antibacterial activities of SLs and several derivatives were tested against a selection of standard bacterial isolates using the broth microdilution method. The SL derivatives tested did not show any significant antibacterial activity in vitro when tested at clinically relevant concentrations. Most likely the reported decrease of mortality rate in the rat septic shock model was not secondary to antibacterial activity of SLs. The SLs may be used as anti-inflammatory agents or immunomodulators wi... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174109 Wed, 11 Feb 2009 01:15:01 +0100 2174109 http://www.medworm.com/index.php?rid=2174108&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201744%26dopt%3DAbstract Authors: Chowdhury P, Soulsby ME, Scott JL We investigated the effects of hindlimb unloading (HLU) on malondialdehyde (MDA), a biomarker for oxidative stress, and glutathione (GSH) levels in tissues of rats. Aminoguanidine (AG), a nucleophilic hydralazine compound and an in vivo antioxidant against reactive oxygen species (ROS) and lipid peroxidation, was used to confirm the HLU-induced oxidative response. Three groups of rats were used: Group 1 was a loaded control group that was maintained on drinking water only; Groups 2 and 3 were hindlimb unloaded (HLU) groups that were maintained on drinking water and on AG in drinking water, respectively. The hindlimb unloaded rats maintained on tap water had significantly elevated MDA levels in 7 tissues (brain, lung, pancreas, kidney, intestin... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174108 Wed, 11 Feb 2009 01:15:01 +0100 2174108 http://www.medworm.com/index.php?rid=2174107&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201745%26dopt%3DAbstract In this study, we explored the early expression of Homer1 gene in the spinal dorsal horn by using the neuropathic pain model of chronic compression of dorsal root ganglion (CCD). The levels of Homer1a mRNA in the ipsilateral dorsal horn of CCD rats were markedly increased at 4 hr, remained elevated at 8 hr, and then returned to baseline values by 24 hr after CCD treatment. In contrast, there were no significant changes of Homer1a expression in the Sham-operated or Control groups. Significant thermal hyperalgesia appeared at 24 hr post-operation in the CCD rats, but not in the Sham-operated or Control groups. These data show that CCD induces a transient and rapid increase in Homer1a expression in the spinal dorsal horn. These data also suggest that the transient and rapid increase in Homer1... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174107 Wed, 11 Feb 2009 01:15:01 +0100 2174107 http://www.medworm.com/index.php?rid=2174106&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201746%26dopt%3DAbstract Authors: Hu ZG, Wang HD, Jin W, Yin HX Ketone bodies have been shown to be favorable alternative metabolic substrates and are protective under neuropathologies. At the same time, cytochrome c release has been reported following traumatic brain injury (TBI) and precipitates apoptosis via the mitochondrial pathway. The present study investigated the effects of a ketogenic diet (KD) on TBI. TBI was produced using the Feeney weight-drop model and the animals were fed either normal diet (ND) or KD. Brain edema was estimated by wet/dry weight ratio; cytochrome c was detected by Western blotting; cellular apoptosis in the penumbra area was examined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and active caspase-3 immunohistochemical staining. The results sh... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174106 Wed, 11 Feb 2009 01:15:01 +0100 2174106 http://www.medworm.com/index.php?rid=2174105&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201747%26dopt%3DAbstract Authors: Jia H, Feng X, Li W, Hu Y, Zeng Q, Liu J, Xu J Neuropathic pain is a complex syndrome resulting from damage to the peripheral nervous system. Central neuroimmune activation contributes to the generation and maintenance of chronic pain after nerve injury. The current study determined the effects of recombinant human erythropoietin (rhEPO) on behavioral hyperalgesia and neuroimmune activation in a rat model of neuropathic pain induced by L5 spinal nerve transection. Animals were randomly assigned into 3 groups: sham-operation with saline; L5 spinal nerve transection with rhEPO (5000 units/kg); or L5 transection with saline. The rhEPO or saline was given ip on the day before surgery and continued daily to day 7 post-transection. The paw pressure threshold and paw withdrawal laten... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174105 Wed, 11 Feb 2009 01:15:01 +0100 2174105 http://www.medworm.com/index.php?rid=2174104&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201748%26dopt%3DAbstract Authors: Hajdu SI PMID: 19201748 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174104 Wed, 11 Feb 2009 01:15:01 +0100 2174104 http://www.medworm.com/index.php?rid=2174103&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19201749%26dopt%3DAbstract Annals of clinical & laboratory science information for authors. Ann Clin Lab Sci. 2009;39(1):95-6 Authors: PMID: 19201749 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=2174103 Wed, 11 Feb 2009 01:15:01 +0100 2174103 http://www.medworm.com/index.php?rid=1943962&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988924%26dopt%3DAbstract The objective of this paper is to clarify the role of mitochondrial dysfunction in cancer morphogenesis and to elucidate how faulty morphogen gradient signaling and inflammatory mediators that regulate OXPHOS can cause cancer-induced morphogenesis. Developmental morphogenesis and cancer morphogenesis are regulated by morphogenetic fields. The importance of morphogenetic fields is illustrated by transplantation of metastatic melanoma cells into the chick-embryo; the tumor cells adapt morphologies that resemble normal cells and function normally in the host. A morphogen gradient is a simple form of morphogenetic field. Morphogens such as those of the transforming growth factor (TGF)-beta family inhibit and stimulate basic cell proliferation at high and low concentrations respectively. Along ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943962 Sat, 08 Nov 2008 15:19:08 +0100 1943962 http://www.medworm.com/index.php?rid=1943961&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988925%26dopt%3DAbstract Authors: Lee J, Kim Y, Lim J, Kim M, Han K Granulocyte-colony stimulating factor (G-CSF) and granulocyte-macrophage-CSF (GM-CSF) are the principal cytokines in granulopoiesis and differentiation of granulocytic precursors. Their physiologic effects are mediated by binding to specific cell surface receptors (G-CSFr and GM-CSFr, respectively), which are widely expressed from immature bone marrow cells to mature peripheral granulocytes. The fact that concentrations of plasma G-CSF and GM-CSF and their receptors are changed in infectious diseases showing neutrophilia is known, but such changes in patients with chronic myelogenous leukemia (CML) have not been studied. Based on quantitative assays of plasma G-CSF and GM-CSF and their receptors on the peripheral granulocytes of CML patients a... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943961 Sat, 08 Nov 2008 15:19:08 +0100 1943961 http://www.medworm.com/index.php?rid=1943960&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988926%26dopt%3DAbstract We describe a SYBR-Green real-time multiplex PCR assay to screen for transcripts TEL-AML1, E2A-PBX1, MLL-AF4, and the two breakpoints of BCR-ABL (p190 and p210). Validation of the assay was based on conventional karyotyping results. This new assay provides a rapid, sensitive, and accurate detection method for prognostically important transcripts in childhood ALL. PMID: 18988926 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943960 Sat, 08 Nov 2008 15:19:08 +0100 1943960 http://www.medworm.com/index.php?rid=1943959&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988927%26dopt%3DAbstract Authors: Ding YS, Wang HD, Tang K, Hu ZG, Jin W, Yan W Vascular endothelial growth factor (VEGF) is a regulator of angiogenesis, vasculogenesis, and vascular permeability. Recent reports suggest that VEGF may play a critical role in formation of peritumoral brain edema (PTBE) associated with meningiomas. While VEGF expression has been shown in meningiomas, studies have not focused on VEGF in the adjacent peritumoral brain regions. The present study examined the protein and gene expression of VEGF in human meningiomas and peritumoral brain areas. Biopsies were obtained from 37 patients. Immunohistochemical staining and immunoblotting were performed to detect the expression of VEGF protein. Reverse-transcriptase polymerase chain reaction (RT-PCR) was used to analyze the presence and quan... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943959 Sat, 08 Nov 2008 15:19:08 +0100 1943959 http://www.medworm.com/index.php?rid=1943958&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988928%26dopt%3DAbstract Authors: Rodriguez-Paris J, Ballay C, Inserra M, Stidham K, Colen T, Roberson J, Gardner P, Schrijver I Using the Hereditary Hearing Loss arrayed primer extension (APEX) array, which contains 198 mutations across 8 hearing loss-associated genes (GJB2, GJB6, GJB3, GJA1, SLC26A4, SLC26A5, 12S-rRNA, and tRNA Ser), we compared the frequency of sequence variants in 94 individuals with early presbycusis to 50 unaffected controls and aimed to identify possible genetic contributors. This cross-sectional study was performed at Stanford University with presbycusis samples from the California Ear Institute. The patients were between ages 20 and 65 yr, with adult-onset sensorineural hearing loss of unknown etiology, and carried a clinical diagnosis of early presbycusis. Exclusion criteria comprise... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943958 Sat, 08 Nov 2008 15:19:08 +0100 1943958 http://www.medworm.com/index.php?rid=1943957&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988929%26dopt%3DAbstract Authors: Lee JH, Lee W, Kwon OH, Kim JH, Kwon OW, Kim KH, Lim JB To evaluate the usefulness of cytokine levels of peripheral blood in diabetic retinopathy (DR), demographic and biochemical parameters including low-density lipoprotein (LDL) diameter as well as cytokine profiles were analyzed in 74 patients with type 2 diabetes mellitus (DM), with DR (n=46) or without DR (n=28). DM duration was longer in the patients with DR than without (p<0.001). Serum glucose (p=0.005) and total cholesterol (p=0.029) levels were higher in DM patients with DR than DM patients without DR. Plasma LDL diameter, interleukin-6 (IL-6), and interleukin-8 (IL-8) showed significant differences among the different degrees of DR severity in analysis of variance (ANOVA) with no definite trend. The risk of DR in... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943957 Sat, 08 Nov 2008 15:19:08 +0100 1943957 http://www.medworm.com/index.php?rid=1943956&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988930%26dopt%3DAbstract In conclusion, we found that the decrease of ADADMTS13 activity in consumptive coagulopathy has stronger relationship to platelet dynamics than has generally been recognized. PMID: 18988930 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943956 Sat, 08 Nov 2008 15:19:08 +0100 1943956 http://www.medworm.com/index.php?rid=1943955&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988931%26dopt%3DAbstract Authors: Drews SJ, Blair J, Lombos E, DeLima C, Burton L, Mazzulli T, Low DE The Seeplex RV Detection kit was used to identify specific respiratory viruses from specimens collected during respiratory outbreaks in the Greater Toronto Area from 1 September 2007 to 1 February 2008. Two hundred-thirty-one patient samples (nasopharyngeal swabs) were collected from 63 respiratory outbreaks. The distribution of outbreaks characterized by molecular means was: 30% (n=19) no identification; 52.5% (n=33) one pathogen; 14.5% (n=9) two pathogens; and 3% (n=2) three pathogens. In contrast, culture-based protocols identified pathogens in fewer outbreaks: 63 % (n=40) no identification; 35% (n=22) 1 pathogen; and 2% (n=1) 2 pathogens (p<0.05). Compared to virus isolation, molecular testing identifie... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943955 Sat, 08 Nov 2008 15:19:08 +0100 1943955 http://www.medworm.com/index.php?rid=1943954&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988932%26dopt%3DAbstract We describe one of these rare cases and review the literature on clinicopathologic sequelae of lay attempts at cosmetic procedures using commercially available silicone preparations. PMID: 18988932 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943954 Sat, 08 Nov 2008 15:19:08 +0100 1943954 http://www.medworm.com/index.php?rid=1943953&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988933%26dopt%3DAbstract We describe a patient with congenital hyperinsulinism with previously unreported pathological findings including normal to decreased number of insulin-positive cells with very few enlarged nuclei, aberrant distribution of glucagon-positive cells, and a non-insulin producing adenomatous focus of unusual morphology. Molecular analysis showed that the patient was a compound heterozygote for two mutations of the ABCC8 gene: a previously unreported nonsense mutation (R841X) and a missense mutation (D1471N) that has been previously described. This case suggests that abnormal function of ABCC8 may result in aberrant pancreatic development. PMID: 18988933 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943953 Sat, 08 Nov 2008 15:19:08 +0100 1943953 http://www.medworm.com/index.php?rid=1943952&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988934%26dopt%3DAbstract We report a case of spontaneous splenic rupture of a 56-yr-old man with acute Babesia microti infection. PMID: 18988934 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943952 Sat, 08 Nov 2008 15:19:08 +0100 1943952 http://www.medworm.com/index.php?rid=1943951&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988935%26dopt%3DAbstract We report a human infection in a patient with a permacath for chronic hemodialysis who was found to have bacteremia caused by P. thiaminolyticus, which is an environmental bacterium that has never been found to cause human disease. We identified this bacterium by biochemical tests, cloning, sequencing the genomic DNA encoding its 16S rRNA, growth characteristics, and electron microscopic studies. This constitutes the first report of a human infection caused by this organism. PMID: 18988935 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943951 Sat, 08 Nov 2008 15:19:08 +0100 1943951 http://www.medworm.com/index.php?rid=1943950&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988936%26dopt%3DAbstract Authors: Yu D, Chen Y, Pan Y, Li H, McCormac MA, Tang YW An unusual staphylococcal isolate was recovered from blood cultures in a patient with chronic hepatitis B virus infection, who presented with low grade fever accompanied by increased upper abdominal pain, nausea and weakness. The isolate was identified as Staphylococcus gallinarum based on biochemical characteristics and 16S rRNA gene sequence analyses. PMID: 18988936 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943950 Sat, 08 Nov 2008 15:19:08 +0100 1943950 http://www.medworm.com/index.php?rid=1943949&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988937%26dopt%3DAbstract We describe the morphologic, immunophenotypic, cytogenetic, and molecular findings in this patient. PMID: 18988937 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943949 Sat, 08 Nov 2008 15:19:08 +0100 1943949 http://www.medworm.com/index.php?rid=1943948&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988938%26dopt%3DAbstract Authors: Park HI, Oh EJ, Park YJ, Han K, Yang CW PMID: 18988938 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943948 Sat, 08 Nov 2008 15:19:08 +0100 1943948 http://www.medworm.com/index.php?rid=1943947&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18988939%26dopt%3DAbstract Authors: Hajdu SI, Tang P PMID: 18988939 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1943947 Sat, 08 Nov 2008 15:19:08 +0100 1943947 http://www.medworm.com/index.php?rid=1724163&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715846%26dopt%3DAbstract We examined the state of activation of components of mTOR, Ras/Raf kinase/ERK, and nuclear factor (NF)-kappaB signal transduction pathways, as well as cell cycle protein analyte correlates in GAC cases. Formalin-fixed paraffin-embedded tissue microarray blocks containing samples from 210 cases of GAC were examined. Immunohistochemistry was utilized to detect the following antigens: S100P, upstream stimulator of ERK, and NF-kappaB pathways; phosphorylated (p)-mTOR (Ser 2448), p-ERK-1/2 (Thr 202/Tyr 204), and one of their common down-stream effectors, p-p70S6K(Thr 389); p-NF-kappaBp65(Ser 536); and cell cycle associated proteins, Ki-67, and S phase kinase-associated protein (Skp)2. Immunoreactivity (0 to 4+) of protein expression and compartmentalization were assessed by bright-field microsc... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724163 Fri, 22 Aug 2008 14:25:39 +0100 1724163 http://www.medworm.com/index.php?rid=1724162&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715847%26dopt%3DAbstract We report one case of acute urate nephropathy and one case of chronic urate nephropathy in two pediatric patients. In their renal biopsies, we demonstrate upregulation of kidney injury molecule-1 (KIM-1), a specific injury marker, in proximal tubular cells. This finding implicates a role for proximal tubular injury in urate nephropathy. PMID: 18715847 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724162 Fri, 22 Aug 2008 14:25:39 +0100 1724162 http://www.medworm.com/index.php?rid=1724161&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715848%26dopt%3DAbstract Authors: Moon Y, Park G, Han K, Kang CS, Lee W Immunohistochemistry (IHC) is widely used in diagnostic practice and research, but it is limited due to its subjective nature and weakness in reproducibility. For successful interpretation, IHC requires an internal reference system that controls for procedural variables and provides a staining intensity reference. We investigated the feasibility of using mouse spleen tissue as an intensity reference in conventional IHC. Formalin-fixed, paraffin-embedded mouse (BALB/c) spleen tissue was stained with variable procedural conditions including primary antibody (Ab) types, antigen retrieval methods, chromogen exposure times, and secondary Ab concentrations. Mouse spleen tissue showed identical staining intensity regardless of primary Ab types, e... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724161 Fri, 22 Aug 2008 14:25:39 +0100 1724161 http://www.medworm.com/index.php?rid=1724160&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715849%26dopt%3DAbstract Authors: Jin W, Zhu L, Guan Q, Chen G, Wang QF, Yin HX, Hang CH, Shi JX, Wang HD Inflammatory response plays an important role in the pathogenesis of acute lung injury (ALI) after traumatic brain injury (TBI). Nuclear factor erythroid 2-related factor 2 (Nrf2) is a key transcription factor that plays a crucial role in cytoprotection against inflammation. The present study explored the influence of Nrf2 genotype on the production of cytokines and on activation of transcription factors in a murine TBI model. Wild-type Nrf2 (+/+) and Nrf2 (-/-) deficient mice were subjected to a moderately severe weight-drop impact-acceleration head injury. Lung wet/dry weight ratio, tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), intercellular adhesion molecul... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724160 Fri, 22 Aug 2008 14:25:39 +0100 1724160 http://www.medworm.com/index.php?rid=1724159&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715850%26dopt%3DAbstract The objective of this study was to evaluate the prevalence of NAG acidosis, characterize the laboratory findings, and determine its impact on clinical outcomes. Acid-base and electrolyte status and clinical outcomes were examined over a 1-yr (2005 calendar yr) period in 109 adult patients (38 males, 71 females; age range 21 to 91 yr) hospitalized for asthma exacerbation. The cohort was divided into 3 groups: I. No metabolic acidosis (n = 66), II. Anion gap (AG) acidosis (n = 11), and III. NAG acidosis (n = 32). For each of the groups, laboratory findings were consistent, demonstrating a tendency to hyperchloremia in the NAG acidosis group. One subject in the NAG acidosis group died. NAG acidosis was associated with a statistically significant (p = 0.028) risk of requirement for mechanical ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724159 Fri, 22 Aug 2008 14:25:39 +0100 1724159 http://www.medworm.com/index.php?rid=1724158&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715851%26dopt%3DAbstract This study investigated the use of corrected 17-alpha-hydroxyprogesterone (17-OHP) values to detect congenital adrenal hyperplasia (CAH) in newborn infants. 17-OHP concentrations in blood spots from 913 neonates were measured using a neonatal screening test. A prematurity index was calculated using a scoring system based on gestational age and birth weight. Blood spot 17-OHP concentrations divided by the sum of prematurity scores were defined as the corrected 17-OHP values. Preterm infants (<30 wk) and low birth weight infants (<1.0 kg) showed 3.9- and 3.8-fold higher blood spot 17-OHP concentrations than normal full term infants. However, no significant differences were observed in the corrected 17-OHP values between the groups. Blood spot 17-OHP levels yielded significant correlati... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724158 Fri, 22 Aug 2008 14:25:39 +0100 1724158 http://www.medworm.com/index.php?rid=1724157&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715852%26dopt%3DAbstract This study investigated the relationship between calculated immature granulocyte (IG) counts and the severity of sepsis. Coagulation parameters, fibrinolytic indices, bacterial isolation rates in blood cultures, and mortality were observed in 237 patients with suspected sepsis. The difference in leukocyte subfractions (delta neutrophil index; DN) identified by a cytochemical myeloperoxidase reaction and by a nuclear lobularity assay was determined with a blood cell analyzer (ADVIA 120, Siemens, Inc.). DN was strongly correlated with manual IG counts (r = 0.75, p <0.05). Patients with high DN (>40%) averaged 40% lower in platelet count, 26% prolongation of prothrombin time (PT), and 35% diminution of antithrombin III (AT III) activity, vs those with low DN (5-20%). Overt disseminated ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724157 Fri, 22 Aug 2008 14:25:39 +0100 1724157 http://www.medworm.com/index.php?rid=1724156&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715853%26dopt%3DAbstract Authors: Cho YU, Jang S, Park CJ, Chi HS The PFA-100 is an in vitro system for measurement of platelet function in whole blood. To establish reference intervals in the Korean population, we investigated the relationships between PFA-100 closure times and gender, ABO blood group, citrate concentration, and time of blood collection in 120 well-characterized healthy Korean adults. Gender did not affect closure time values. Blood group O was associated with longer collagen/epinephrine (CEPI) and collagen/ADP (CADP) closure times than non-O groups (p <0.0001 for both). Closure times were shorter in samples obtained in the morning vs the afternoon. Regression analysis showed an association between CEPI and CADP closure times (p <0.0001), but there were no associations between PFA-100 r... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724156 Fri, 22 Aug 2008 14:25:39 +0100 1724156 http://www.medworm.com/index.php?rid=1724155&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715854%26dopt%3DAbstract Authors: Lee S, Park YJ, Yoo JH, Kahng J, Jeong IH, Kwon YM, Han K To compare the culture screening protocols for methicillin-resistant Staphylococcus aureus (MRSA), a total of 300 duplicate nasal swabs (233 initial cultures and 67 weekly follow-up cultures) were collected consecutively from 233 patients in the Intensive Care Unit (ICU). One swab was plated directly on MRSA-Select agar (D-MRSA-Select) and observed at 24 hr. The duplicate swab was incubated in tryptic soy broth (TSB) with 6.5% NaCl for 24 hr, and then subcultured on MRSA-Select (B-MRSA-Select), BAP (B-BAP), and mannitol salt agar with 4 mg/L oxacillin (B-MSA(OXA)), and observed at 24 hr. MRSA was detected in 13.7% (32/233) of the initial and 22.4% (15/67) of the follow-up specimens. A patient was classified as MRSA-posi... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724155 Fri, 22 Aug 2008 14:25:39 +0100 1724155 http://www.medworm.com/index.php?rid=1724154&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715855%26dopt%3DAbstract In this study, real-time PCR-melting curve analysis at nucleotide 1227 of RHD exon 9 was performed on 990 leftover blood samples. PCR analysis identified 22 samples with the 1227G+A pattern, 965 samples with the 1227G pattern, and 3 with negative real-time results. The RHDEL allele frequency is 0.0116 (22/1980) among Taiwanese. These real-time PCR patterns were validated through DNA sequencing analyses of RHD exon 9 on 22 samples with the 1227G+A pattern and on 50 randomly selected samples from 1227G individuals. The real-time PCR test was then analyzed in 118 apparently RhD-negative Taiwanese donors, including 38 D(el) and 80 true RhD-negative donors, for efficiency studies. All of the D(el) samples (38, 100%) were found to have the 1227A pattern. Among the 80 serologic true RhD-negative ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724154 Fri, 22 Aug 2008 14:25:39 +0100 1724154 http://www.medworm.com/index.php?rid=1724153&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715856%26dopt%3DAbstract Authors: Chen DP, Tseng CP, Tsai SH, Wu TL, Chang PY, Sun CF Post-transplantation chimerism testing is important to monitor the engraftment of donor stem cells and for the diagnosis of relapse. Detecting the presence of donor/recipient-specific short tandem repeats (STRs) is a frequently used method for engraftment study. Unfortunately, the interpretation of the STR-based chimerism tests is often subject to interference by the presence of a stutter peak, which is one 4-base repeat unit smaller than an authentic allele. The aim of this study was to systematically analyze and resolve the effect of stutter peaks on the interpretation of STR-based chimerism tests. The AmpFlSTR Identifiler Amplification kit (Applied Biosystems)was used to amplify 15 STR loci using genomic DNA from 30 random... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724153 Fri, 22 Aug 2008 14:25:39 +0100 1724153 http://www.medworm.com/index.php?rid=1724152&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715857%26dopt%3DAbstract We report a case of profound hemolytic anemia in a liver-transplant eligible patient who was diagnosed with cirrhosis secondary to non-alcoholic steatohepatitis (NASH). The patient initially was treated with red cell transfusion, iv immunoglobulin, and steroids. He developed acute renal failure that required dialysis. Subsequent management included plasmapheresis and rituximab therapy. The patient developed hepatorenal syndrome and died from progressive hepatic failure. To our knowledge, this is the first report of an association between NASH and WAIHA. PMID: 18715857 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724152 Fri, 22 Aug 2008 14:25:39 +0100 1724152 http://www.medworm.com/index.php?rid=1724151&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715858%26dopt%3DAbstract We report a patient with Budd-Chiari syndrome and a history of heparin-induced thrombocytopenia who presented for orthotopic liver transplant and required postoperative anticoagulation with bivalirudin. During the post-transplant graft function improvement, we observed a significant dose-effect alteration manifested by an increased bivalirudin dose requirement as factor V activity increased. This observation is an important consideration in the attempt to maintain an optimal balance between effective anticoagulation and a reduced risk of postoperative bleeding. PMID: 18715858 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724151 Fri, 22 Aug 2008 14:25:39 +0100 1724151 http://www.medworm.com/index.php?rid=1724150&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715859%26dopt%3DAbstract Authors: Chung HJ, Chi HS, Cho YU, Park CJ, Seo EJ, Kim KH, Lee JH A 32-yr-old man with the chronic phase of chronic myeloid leukemia (CML-CP) was treated with imatinib mesylate for 6 mo. The real-time quantitative reverse transcription PCR ratio for BCR/ABL in blood mRNA (BCR/ABL RT-QPCR) decreased from an initial value of 0.0159 to a low value of 0.0012 after 3 mo, indicating complete hematologic response. During the next 3 mo, the patient progressed to a promyelocytic blast crisis, displaying leukemic cells containing both BCR/ABL and PML/RARalpha chimeric mRNAs. Complete remission was achieved by therapy with all-trans retinoic acid (ATRA) and high-dose imatinib mesylate. Using retrospective PML/RARalpha RT-QPCR with a bone marrow specimen obtained at the initial diagnosis of CML-C... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724150 Fri, 22 Aug 2008 14:25:39 +0100 1724150 http://www.medworm.com/index.php?rid=1724149&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715860%26dopt%3DAbstract We report a papillary glioneuronal tumor occurring in the right frontal lobe of a 26-yr-old woman and we review the pertinent literature. Papillary glioneuronal tumor (PGNT) is a rare cerebral neoplasm, identified in approximately 37 cases to date. In 2007, the World Health Organization (WHO) classified the PGNT as a grade I neuronal-glial tumor because of its biphasic neurocytic and glial components and indolent clinical course. Patients commonly present with headaches or seizures, but may be asymptomatic with the mass discovered incidentally upon neuroimaging. Histology demonstrates a pseudopapillary architecture with a single or a pseudostratified layer of glial cells overlying hyalinized vasculature with interpapillary regions of neurocytic or ganglion cells. Peripheral eosinophilic gr... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724149 Fri, 22 Aug 2008 14:25:39 +0100 1724149 http://www.medworm.com/index.php?rid=1724148&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715861%26dopt%3DAbstract Authors: Zhang Y, Wang J, Wilson GJ, Tang YW, Lu HZ In a 16-mo-old infant born to an HIV-infected mother, repeatedly negative results of a HIV rapid antibody test had been reported during the past 6 mo. The infant presented with several HIV-defining illnesses and HIV RT-PCR testing confirmed the presence of HIV infection. There are at least 2 possible explanations for the child's false-negative rapid HIV test results: First, his primary antibody production may have been suppressed by the presence of maternal IgG antibodies. Second, his mother was highly immunosuppressed, so that the low level of maternally derived IgG was only detected by HIV-EIA and Western blot. Our data suggest that the HIV rapid antibody test may not be sufficiently sensitive to detect HIV antibodies in infants age... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724148 Fri, 22 Aug 2008 14:25:39 +0100 1724148 http://www.medworm.com/index.php?rid=1724147&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715862%26dopt%3DAbstract Authors: Hajdu SI, Ehya H PMID: 18715862 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724147 Fri, 22 Aug 2008 14:25:39 +0100 1724147 http://www.medworm.com/index.php?rid=1724146&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18715863%26dopt%3DAbstract Authors: Hawker CD, Knight JA, Sunderman FW PMID: 18715863 [PubMed - as supplied by publisher] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1724146 Fri, 22 Aug 2008 14:25:39 +0100 1724146 http://www.medworm.com/index.php?rid=1447069&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316774%26dopt%3DAbstract Authors: Hajdu SI, Ali SZ PMID: 18316774 [PubMed - indexed for MEDLINE] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447069 Fri, 16 May 2008 17:02:04 +0100 1447069 http://www.medworm.com/index.php?rid=1447068&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316775%26dopt%3DAbstract Authors: Kahng J, Park HH, Han K, Choi BY, Lee W The numbers of antibody-binding sites of platelet glycoprotein (GP) IIb/IIIa on circulating platelets were analyzed using 4 kinds of antibodies in 34 aplastic anemia (AA) patients, 20 idiopathic thrombocytopenic purpura (ITP) patients, and 14 normal controls. The numbers of antibody-binding sites of CD41, CD41a, CD41b, and CD61 on platelets of the AA patients were less than in the normal controls (p <0.001). In the ITP patients, the numbers of sites for CD41 and CD41a were less than in normal controls (p <0.05). There were significant positive correlations between CD41 and CD41a, CD41b, and CD61 in the 3 groups. There were significant negative correlations between CD41 and CD41b and between CD41a and CD41b in the normal controls, b... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447068 Fri, 16 May 2008 17:02:04 +0100 1447068 http://www.medworm.com/index.php?rid=1447067&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316776%26dopt%3DAbstract We report a Korean kindred in whom FNDI is associated with a novel deletion mutation in exon 2 of the AVP-NPII gene encoding the neurophysin II moiety. An 18-yr-old man with polyuria and polydipsia was shown to have central diabetes insipidus by using the water deprivation test. Four family members were suspected to have symptomatic vasopressin-deficient diabetes insipidus. Direct sequencing of the AVP-NPII gene showed a heterozygous GAG deletion mutation in exon 2, which results in in-frame deletion of glutamic acid (c.232_234delGAG; p.Glu78del). The mutation was predicted to yield an abnormal AVP precursor lacking Glu78 (E78) in its neurophysin II moiety. Because Glu78 is essential for neurophysin II molecules to form a salt bridge with AVP, the function of neurophysin as a carrier prote... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447067 Fri, 16 May 2008 17:02:04 +0100 1447067 http://www.medworm.com/index.php?rid=1447066&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316777%26dopt%3DAbstract Authors: Xuan D, Li S, Zhang X, Hu F, Lin L, Wang C, Zhang J Cleidocranial dysplasia (CCD) is an autosomal dominant inheritable skeletal disease caused by heterozygous mutations in an osteoblast-specific transcription factor, RUNX2. Mutational analyses of RUNX2 were done on 4 unrelated Chinese patients with CCD. One nonsense and 3 missense mutations were detected, including one novel mutation, a heterozygous G to C transition mutation at nucleotide 475 in exon 2, which converts glycine to arginine at codon 159 (G159R). Two mutations, R225W and R391X, were reported in Chinese patients with CCD for the first time. Our findings show that R225 mutations interfere with nuclear accumulation of RUNX2 protein, and that a lack of nuclear RUNX2 protein accumulation is at least one of the causes ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447066 Fri, 16 May 2008 17:02:04 +0100 1447066 http://www.medworm.com/index.php?rid=1447065&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316778%26dopt%3DAbstract Authors: Chen H, Qiu Y, Chen L, Li L, Chen J, Zhang C, Wang B, Yu Y, Zhu Z, Zhu F, Qian B, Ma W The goal of this study was to determine if neurofibromin is expressed in cultured human osteoblasts and chondrocytes that were isolated from ilia and ilial growth plate. Purity of the osteoblast and chondrocyte cultures was confirmed by alkaline phosphatase and toluidine blue staining, respectively. The reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect neurofibromin mRNA. Indirect immunofluorescence and Western blot studies were done to delineate the cellular distribution and expression levels of neurofibromin. These experiments show that neurofibromin is expressed at low levels in human osteoblasts and chondrocytes and is located mainly in the cytoplasm. Only ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447065 Fri, 16 May 2008 17:02:04 +0100 1447065 http://www.medworm.com/index.php?rid=1447064&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316779%26dopt%3DAbstract In conclusion, this study validates the accuracy of Roche's 2nd generation assay, which is substantially improved over Roche's 1st generation HbA1c assay. PMID: 18316779 [PubMed - indexed for MEDLINE] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447064 Fri, 16 May 2008 17:02:04 +0100 1447064 http://www.medworm.com/index.php?rid=1447063&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316780%26dopt%3DAbstract This study evaluated the performance of a handheld coagulation analyzer for measurements of capillary blood specimens of 93 outpatient cardiology patients with atrial fibrillation who were receiving oral anti-coagulant therapy. The international normalized ratio (INR) results of the CoaguChek XS system (Roche Diagnostics) were compared with those obtained in the central laboratory with citrated venous blood specimens using the ACL9000 coagulation analyzer (Instrumentation Laboratory). The INR results for prothrombin time by the CoaguChek XS analyzer were closely correlated with the central laboratory's results in the INR range of 0.96 approximately 8.53 (r = 0.964). A statistically significant difference was noted between 2 lots of test strips, but the difference was miniscule (mean +/- 95... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447063 Fri, 16 May 2008 17:02:04 +0100 1447063 http://www.medworm.com/index.php?rid=1447062&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316781%26dopt%3DAbstract In conclusion, S-RV is reliable, rapid, relatively easy to perform, and able to detect more than 1 virus simultaneously. Therefore, implementation of the S-RV assay in clinical laboratories will aid rapid diagnosis and treatment of major viral infections of the respiratory tract. PMID: 18316781 [PubMed - indexed for MEDLINE] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447062 Fri, 16 May 2008 17:02:04 +0100 1447062 http://www.medworm.com/index.php?rid=1447061&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316782%26dopt%3DAbstract In this study, we investigate the nature of the JNK-induced phosphorylation of raf. In in vitro experiments in which immunobead-bound raf is phosphorylated by activated JNK, we find strong phosphorylation signals at raf-Ser259 and Ser338. The Ser259 phosphorylation is surprising since it is associated with inhibition of migration of raf to the cell membrane where it can interact with ras-p21. We also find that in oocytes induced to mature with oncogenic ras-p21, which induces high levels of phosphorylated JNK and MAPK, the same pattern of phosphorylation of raf occurs. In contrast, in oocytes induced to mature with insulin, which requires activation of wild-type ras-p21, phosphorylation of raf-Ser338 but not raf-Ser259 occurs. In oncogenic ras-transformed human pancreatic cancer MIA-PaCa-2... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447061 Fri, 16 May 2008 17:02:04 +0100 1447061 http://www.medworm.com/index.php?rid=1447060&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316783%26dopt%3DAbstract Authors: Zhang PL, Lun M, Schworer CM, Blasick TM, Masker KK, Jones JB, Carey DJ Renal injury is known to trigger upregulation of many intracellular signal proteins, but those most sensitive in responding to renal injury remain debatable. We used gene microarray analysis to compare gene expression in rat kidneys subjected to early ischemia-reperfusion injury (30 min of renal ischemia and 3 hr of reperfusion) with non-ischemic kidneys as controls. Among 31,100 genes analyzed, microarray analysis revealed 21 genes with >3-fold increase in expression in ischemic kidneys compared to control non-ischemic kidneys. These upregulated genes included heat shock protein 70 (43-fold), heat shock protein 27 (12-fold), heme oxygenase-1 (10-fold), kidney injury molecule-1 (8-fold), and several sub... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447060 Fri, 16 May 2008 17:02:04 +0100 1447060 http://www.medworm.com/index.php?rid=1447059&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316784%26dopt%3DAbstract This study investigated whether progesterone administration modulates toll-like receptors (TLRs) and the nuclear factor-kappa B (NF-kappaB) signaling pathway in the injured rat brain following traumatic brain injury (TBI). Right parietal cortical contusion was made by a weight-dropping method. Male rats were given 0 or 16 mg/kg injections of progesterone at postinjury hr 1 and 6 and on days 1, 2, 3, 4, and 5. Brain samples were extracted at 5 days after trauma. We measured mRNA expression of TLR2 and TLR4 by reverse-transcriptase polymerase chain reaction (RT-PCR), NF-kappaB binding activity by electrophoretic mobility shift assay (EMSA), concentrations of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6) by enzyme-linked immunosorbent assay ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447059 Fri, 16 May 2008 17:02:04 +0100 1447059 http://www.medworm.com/index.php?rid=1447058&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316785%26dopt%3DAbstract Authors: Moon Y, Kim M, Park G Burkitt lymphomas (BLs) frequently show secondary chromosomal abnormalities. Here, the authors describe a case of BL with an unusual dual translocation of chromosome 14, t(8;14) and t(14;15), and partial duplication of 1q. This 5-yr-old female patient had several unfavorable prognostic factors including elevated serum lactate dehydrogenase activity and involvement of the central nervous system and bone marrow. Despite receiving CCG-106A chemotherapy, she was resistant to therapy and died on the 70th hospital day. To our knowledge, this is the first documented case report of BL harboring dual translocation of chromosome 14 involving chromosomes 8 and 15, which may be a factor associated with unfavorable clinical course. PMID: 18316785 [PubMed - indexed... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447058 Fri, 16 May 2008 17:02:04 +0100 1447058 http://www.medworm.com/index.php?rid=1447057&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316786%26dopt%3DAbstract We report a Korean neonate with severe hemolytic disease of the newborn caused by anti-Di(b). The phenotype and genotype of the Diego blood group system of the patient and his mother were Di(a+b+) and Di(a+b-), respectively. The mother's serum and eluate from the neonate's erythrocytes contained anti-Di(b). This case was successfully managed with phototherapy and high dose iv immunoglobulin. Since most commercial antibody detection panels do not contain Di(b-) red cells, it is important to consider anti-Di(b) in cases of hemolytic disease of the newborn caused by an antibody against a high frequency antigen. PMID: 18316786 [PubMed - indexed for MEDLINE] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447057 Fri, 16 May 2008 17:02:04 +0100 1447057 http://www.medworm.com/index.php?rid=1447056&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316787%26dopt%3DAbstract Authors: HooKim K, deRoux S, Igbokwe A, Stanek A, Koo J, Hsu J, Pincus MR, Bluth MH Giant cell myocarditis, a rare, fatal, and poorly understood cause of myocarditis, requires pathological examination for diagnosis. It is considered to be an autoimmune disease and is frequently associated with other conditions, in particular thymoma and myasthenia gravis. The typical patient with giant cell myocarditis is young and has severe, progressive congestive cardiac failure that is unresponsive to standard medical therapy and ultimately requires cardiac transplantation. Hence giant cell myocarditis is the most dangerous form of myocarditis. Here we report an unusual presentation of giant cell myocarditis, which mimicked acute myocardial infarction in an elderly woman with myasthenia gravis and ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447056 Fri, 16 May 2008 17:02:04 +0100 1447056 http://www.medworm.com/index.php?rid=1447055&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18316788%26dopt%3DAbstract Authors: Jun YH, Nahm CH, Choi JW PMID: 18316788 [PubMed - indexed for MEDLINE] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447055 Fri, 16 May 2008 17:02:04 +0100 1447055 http://www.medworm.com/index.php?rid=1447054&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469353%26dopt%3DAbstract In conclusion, D2-40 antibody staining may be useful as an adjunct in detecting LVI in invasive lobular carcinoma, especially in node-negative patients with the classic variant of invasive lobular carcinoma. PMID: 18469353 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447054 Fri, 16 May 2008 17:02:04 +0100 1447054 http://www.medworm.com/index.php?rid=1447053&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469354%26dopt%3DAbstract Authors: Yang J, Li Y, Khoury T, Alrawi S, Goodrich DW, Tan D Nuclear matrix proteins (NMPs) are important diagnostic and prognostic markers in various human cancers. The hHpr1/p84/Thoc1 protein, a key NMP, resides in the nuclear matrix and is involved in the human TREX complex, which is required for regulation of transcription elongation, pre-RNA splicing, and mRNA export of a subset of human genes. Depletion of hHpr1/p84/Thoc1 decreases growth rates in multiple cancer cell lines, and the expression levels of hHpr1/p84/Thoc1 are strongly associated with tumor size and aggressiveness of several human cancers. Little is known about the expression of this protein in human non-small cell lung cancer (NSCLC) and its association with patients' clinicopathologic characteristics and prognosis... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447053 Fri, 16 May 2008 17:02:04 +0100 1447053 http://www.medworm.com/index.php?rid=1447052&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469355%26dopt%3DAbstract Authors: Zhang D, Zhou Y, Wu L, Wang S, Zheng H, Yu B, Li J The purpose of this study was to determine the predictive value of IL-6-572, -190, and -634 gene polymorphisms on the cachexia susceptibility and survival time of patients with pancreatic cancer. Genotypes were determined using restriction fragment length polymorphism analysis of polymerase chain reaction products. All of the subjects were homozygous to the C allele of IL-6-190. No significant differences were found in the IL-6-572G and -190C distributions between the cachexia group and the non-cachexia group. Patients with cachexia showed significantly higher prevalence of common bile duct obstruction (CBD) than those without (p = 0.026). A significantly increased frequency of the IL-6-634G allele was noted in the cachexia gr... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447052 Fri, 16 May 2008 17:02:04 +0100 1447052 http://www.medworm.com/index.php?rid=1447051&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469356%26dopt%3DAbstract This study documents a significant disturbance of pro-oxidative/antioxidative balance in the plasma of patients undergoing allogeneic HSCT regardless of the intensity of the conditioning regimen. PMID: 18469356 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447051 Fri, 16 May 2008 17:02:04 +0100 1447051 http://www.medworm.com/index.php?rid=1447050&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469357%26dopt%3DAbstract This study shows that the optical platelet aggregation test using arachidonic acid gave an accurate assessment of the response to aspirin, and that results of the PFA-100 test using the CEPI cartridge correlated well with results of the optical platelet aggregation test. PMID: 18469357 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447050 Fri, 16 May 2008 17:02:04 +0100 1447050 http://www.medworm.com/index.php?rid=1447049&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469358%26dopt%3DAbstract Authors: Talwalkar SS, Bon-Homme M, Miller JJ, Elin RJ Ischemia modified albumin (IMA) is a relatively new marker for evaluating patients with cardiac ischemia. Data are emerging on its potential role in non-cardiac ischemic events. In this pilot study we evaluated the utility of IMA in diagnosing acute coronary syndromes (ACS), assessed its role in the diagnosis of non-cardiac ischemia, and correlated its efficacy with troponin T (TnT). Serum levels of IMA were measured in 89 sequential patients who presented to the emergency room with chest pain for which serum TnT was ordered. The patients were classified into 4 groups based on their IMA and TnT results and discharge diagnoses. The data were analyzed with Fischer's exact test. Multivariate logistic regression analysis relating acute... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447049 Fri, 16 May 2008 17:02:04 +0100 1447049 http://www.medworm.com/index.php?rid=1447048&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469359%26dopt%3DAbstract Authors: Smith FB, Elnawawi A Plasma cells are readily identified microscopically after immunostaining for the CD138 antigen, and CD138-stained bone marrow core biopsy sections have proved superior to Giemsa-stained aspirate smears and hematoxylineosin (H&E) sections for determining plasma cell percentages in marrow. The CD138-stained plasma cell percentage is generally obtained by visual estimation, after viewing the entire section. We propose an alternative method, in which the microscopist initially views the immunostained section under low- and medium-power magnification, then selects a single high-power field in which the relative concentration of plasma cells appears most representative of the section as a whole, and performs a manual differential count of plasma cells in tha... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447048 Fri, 16 May 2008 17:02:04 +0100 1447048 http://www.medworm.com/index.php?rid=1447047&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469360%26dopt%3DAbstract We report a case of visceral neuropathy with secondary muscle changes in a 7-yr-old ventilator dependent, mentally retarded child who presented with a history of chronic constipation and symptoms of intestinal obstruction. The muscle layer of the colectomy specimen showed extensive infiltration of vacuolated cells that were positive for S-100 and synaptophysin but negative for glial fibrillary acidic protein (GFAP) and neural filament protein (NFP). Calretinin positivity was preserved in submucosal ganglion cells but was absent in vacuolated nerve branches. Masson's trichrome stain showed evidence of fibrosis, indicative of muscle damage. There was a reduced number of intestinal cells of Cajal in the muscularis propria, as indicated by CD117 (c-kit) immunostaining. This disorder is most li... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447047 Fri, 16 May 2008 17:02:04 +0100 1447047 http://www.medworm.com/index.php?rid=1447046&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469361%26dopt%3DAbstract Authors: Park HI, Min WK, Lee W, Park H, Park CJ, Chi HS, Chun S Physicians need to respond to critical value alerts in a timely and appropriate manner. We evaluated the clinical usefulness of a short message service (SMS) system for notifying physicians of critical values by sending a text message to their personal data assistant (PDA) phones. The clinical response times and clinical response rates to notifications of critical hyperkalemia in inpatients in 2001, when the callback system alone was used to alert physicians, were compared to those in 2005, when the SMS alert system was used in addition to the callback system. The clinical response time to SMS alerts of critical hyperkalemia sent to PDA phones in 2005 was remarkably shorter than the response time to alerts sent using the ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447046 Fri, 16 May 2008 17:02:04 +0100 1447046 http://www.medworm.com/index.php?rid=1447045&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469362%26dopt%3DAbstract We report 2 cases of collision tumors involving amelanotic malignant melanoma of the back. One patient is a 79-yr-old male with an 8.7 x 5.5 x 4.5 cm polypoid lesion that on shave biopsy was diagnosed as basal cell carcinoma. Subsequent excision showed that the lesion was largely composed of amelanotic melanoma underlying a relatively small and thin basal cell carcinoma, and this probably would have been demonstrated in a punch (rather than shave) biopsy. The other patient is a 71-yr-old male with a 1 cm exophytic lesion on the back, which was determined microscopically to be melanoma, and a 0.6 cm papule on the back. This lesion was composed of 2 distinct contiguous neoplastic infiltrates, the predominant component being an atypical fibroxanthoma and the smaller component an amelanotic me... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447045 Fri, 16 May 2008 17:02:04 +0100 1447045 http://www.medworm.com/index.php?rid=1447044&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469363%26dopt%3DAbstract Authors: Nepal M, Mainali R, Schworer CM, Difilippo W, Zhang PL, Schultz MF The nephrotic range of proteinuria is uncommon in scleroderma renal crisis. This 46-yr-old woman with a medical history of scleroderma presented with very high blood pressure, a sudden elevation of serum creatinine, and proteinuria in the nephrotic range. Renal biopsy revealed onion-skin type of arterial changes with necrosis, confirming the presence of scleroderma nephropathy. Electron microscopy showed diffuse fusion of foot processes. Immunohistochemical staining (IHC) revealed increased expression in glomeruli of phosphorylated mammalian target of rapamycin (p-mTOR). These findings suggest that fusion of foot processes and activation of mammalian target of rapamycin-dependent pathways in podocytes are most ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447044 Fri, 16 May 2008 17:02:04 +0100 1447044 http://www.medworm.com/index.php?rid=1447043&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469364%26dopt%3DAbstract Authors: Smith-Norowitz TA, Drew H, Norowitz HM, Nowakowski M, Bluth EF, Durkin HG, Bluth MH Breast milk is a complex fluid, rich in nutrients and non-nutritional bioactive components, including antimicrobial factors, immunoglobulins, cytokines, and anti-inflammatory substances. Although IgE is implicated in viral immunity, its role in breast milk in parvovirus B19 immunity has not been studied. Total immunoglobulin levels of IgE, IgG, and IgE anti-parvovirus B19 antibodies were determined by ELISA and Western blot analysis in breast milk and in sera from a mother and her nursing infant (female, 10 mo). For specific IgE protein determination, breast milk was fractionated by chromatography on G-100 Sephadex; 3 peaks were collected and separated by SDS PAGE. The levels of total IgE in br... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447043 Fri, 16 May 2008 17:02:04 +0100 1447043 http://www.medworm.com/index.php?rid=1447042&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18469365%26dopt%3DAbstract Authors: Hajdu SI PMID: 18469365 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447042 Fri, 16 May 2008 17:02:04 +0100 1447042 http://www.medworm.com/index.php?rid=1447126&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311864%26dopt%3DAbstract Authors: Abo SM, DeBari VA The antiphospholipid syndrome (APS) was first described in 1986. The original association of this hypercoagulable state with anticardiolipin antibodies (aCL) resulted from the synthesis of evidence stemming from laboratory findings in systemic lupus erythematosus (SLE), ie, the frequent occurrence of false-positive VDRL tests and the paradoxical observation of the so-called "lupus anticoagulant" (LA), an increase in phospholipid (PL)-dependent clotting times. By the early 1990s, it was clear that a co-factor was involved in the reaction of antibodies to PL (aPL) in SLE patients with secondary APS and that this was a hitherto-obscure protein, beta-2 glycoprotein I (beta2GPI). In the intervening years, it has been established that beta2GPI and other PL-binding ... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447126 Mon, 01 Jan 2007 05:00:00 +0100 1447126 http://www.medworm.com/index.php?rid=1447125&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311865%26dopt%3DAbstract In conclusion, the prognosis is related to the tumor size and the number of mitoses seen on histological examination. Positive detection of CD117, combined with other markers and pathological features, is of great importance in the differential diagnosis of gastric GISTs. Complete resection with negative margins remains the fundamental objective in the surgical management of gastric GISTs. PMID: 17311865 [PubMed - indexed for MEDLINE] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447125 Mon, 01 Jan 2007 05:00:00 +0100 1447125 http://www.medworm.com/index.php?rid=1447124&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311866%26dopt%3DAbstract Authors: Walker EM, Epling CP, Parris C, Cansino S, Ghosh P, Desai DH, Morrison RG, Wright GL, Wehner P, Mangiarua EI, Walker SM, Blough ER There are few effective agents that safely remove excess iron from iron-overloaded individuals. Our goal was to evaluate the iron-removing effectiveness of acetaminophen given ip or orally in the gerbil iron-overload model. Male gerbils were divided into 5 groups: saline controls, iron-overloaded controls, iron-overloaded treated with ip acetaminophen, iron-overloaded treated with oral acetaminophen, and iron-overloaded treated with ipdeferoxamine. Iron dextran was injected iptwice/wk for 8 wk. Acetaminophen and deferoxamine treatments were given on Mondays, Wednesdays, and Fridays during the same 8 wk and continued for 4 wk after completion of iro... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447124 Mon, 01 Jan 2007 05:00:00 +0100 1447124 http://www.medworm.com/index.php?rid=1447123&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311867%26dopt%3DAbstract Authors: Zhang PL, Quiery AT, Blasick TM, Brown RE Bisphosphonates have been used to treat lytic lesions of multiple myeloma because of their inhibitory effects on osteoclasts. However, their effects on myeloma cells, per se, are not known to be correlated with specific markers. The goal of this study was to assess molecular concomitants of myeloma that might serve as markers for predicting the pharmacologic impact of bisphosphonates on malignant plasma cells. We tested the correlation of serum monoclonal immunoglobulin (Ig) level (IgG and IgA classes) with therapies utilizing two aminobisphosphonates, pamidronate (Aredia) and/or zoledronate (Zometa), in 19 patients with multiple myeloma. Myeloma cells from bone marrow biopsies were immunohistochemically stained for H-ras (p21 ras), N-... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447123 Mon, 01 Jan 2007 05:00:00 +0100 1447123 http://www.medworm.com/index.php?rid=1447122&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311868%26dopt%3DAbstract In this study, the clinicopathological significance of tumor microvessel density (MVD) was assessed in 105 patients with HCC by immunohistochemical staining of CD105, CD34, and vascular endothelial growth factor (VEGF). Moreover, the use of the tissue microarray technique in evaluating angiogenesis of HCC was appraised. The MVD by CD105 immunostaining (MVD-CD105) was significantly lower in larger tumors (5 cm diameter as a cutoff point, p=0.001), more aggressive tumors, as indicated by venous infiltration (present vs absent, p=0.001), and tumors with advanced TNM stage (stage I & II vs stage III, p=0.011). A lower score of MVD by CD34 immunostaining (MVD-CD34) showed significant association only with venous invasion (p<0.001), whereas the MVD by CD105 immunostaining in tissue microa... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447122 Mon, 01 Jan 2007 05:00:00 +0100 1447122 http://www.medworm.com/index.php?rid=1447121&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311869%26dopt%3DAbstract This study investigated the temporal profile of effects of hydroxyethyl starch (HES) 130/0.4 on pulmonary capillary leakage in a rat sepsis model induced by cecal ligation and puncture (CLP). Arterial blood pressure and heart rate were monitored during the experiment. Pulmonary capillary leakage was evaluated at 6, 12, 18, and 24 hr after CLP, and HES 130/0.4 was infused iv 2 hr prior to each time point. Myeloperoxidase (MPO) activity of lung homogenates and pulmonary levels of tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6, IL-10, and nuclear factor-kappaB (NF-kappaB) activity were measured. Infusion of HES 130/0.4 attenuated the pulmonary capillary leakage, reduced the elevations of MPO, TNF-alpha, IL-6, and NF-kappaB levels, and further increased the IL-10 level. Infusion o... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447121 Mon, 01 Jan 2007 05:00:00 +0100 1447121 http://www.medworm.com/index.php?rid=1447120&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311870%26dopt%3DAbstract Authors: Knox-Macaulay H, Gravell D, Elender F Several studies have provided reference ranges for the concentration of serum transferrin receptor (sTfR) in various white populations, but there is a dearth of relevant reference sTfR data in non-whites. The aim of this investigation was to establish sTfR reference ranges and mean values for a healthy non-white Arab population that could be used also for Arabs worldwide. sTfR and serum ferritin concentrations were estimated by immunoassays and blood counts were determined by conventional methods. Analysis of the data of 114 volunteer Arab blood donors (91 male, 23 female) revealed a higher mean sTfR concentration in males of 22.6+/-8.1 nmol/L (range 10.9-38.7 nmol/L) compared to that in females of 18.7+/-4.4 nmol/L (range 10.7-25.8 nmol/L... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447120 Mon, 01 Jan 2007 05:00:00 +0100 1447120 http://www.medworm.com/index.php?rid=1447119&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311871%26dopt%3DAbstract Authors: Lee JY To purify human erythropoietin-binding protein (Epo-bp), the recombinant vector JYL26 was constructed by inserting human Epo-receptor cDNA by PCR into a pGEX-2T plasmid vector with a thrombin cleavage site. EpoRex-th fusion protein, containing glutathione-S-transferase (GST) and Epo-bp, was purified by glutathione-affinity chromatography. Biologically active pure human Epo-bp (MW=29 kDa) was then purified by Epo-agarose chromatography after cleaving off the GST by thrombin. Purified Epo-bp has a strong binding affinity to 125I-Epo, and unlabeled Epo eliminates the binding (p<0.0001). Trypsin digested Epo-bp to approximately 20 kDa and completely eliminated the binding. Thus, Epo-bp ligand binding is specific and it may require an intact Epo-bp. Ligand-binding sites w... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447119 Mon, 01 Jan 2007 05:00:00 +0100 1447119 http://www.medworm.com/index.php?rid=1447118&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311872%26dopt%3DAbstract Authors: Yang BC, Zeng JQ, Yu Q, Liang YL, Su YQ, Deng ZH The ABO blood group system is the most important in transfusion medicine. O blood group is common in Chinese Han people, but the distribution of various O alleles is unknown. Sequences of exon6 and exon7 of the O allele at the ABO gene locus were studied in 100 individuals of the O phenotype randomly selected from the Chinese Han population. Some samples, when required, were cloned and sequenced spanning exon6 and exon7. Eight O alleles were found in the Chinese population. Most have the 2 common O01 or O02 alleles. The allele frequency of ABO*O01 was 0.47, and that of ABO*O02 was 0.495. One individual was found to have O05 allele. Five alleles were found to differ from all alleles reported to date. Four of these alleles differe... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447118 Mon, 01 Jan 2007 05:00:00 +0100 1447118 http://www.medworm.com/index.php?rid=1447117&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311873%26dopt%3DAbstract In this study, the Phoenix system was highly specific (76/77, 98.7%), and it identified 3 additional ESBL-producers that were not detected by DDST. However, the Phoenix system's sensitivity was very low (15/72, 20.8%). Considering the increasing prevalence of ESBL production among AmpC-producers, the BD Phoenix system could not be considered a reliable stand-alone ESBL detection method for the strains tested in our study. PMID: 17311873 [PubMed - indexed for MEDLINE] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447117 Mon, 01 Jan 2007 05:00:00 +0100 1447117 http://www.medworm.com/index.php?rid=1447116&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311874%26dopt%3DAbstract This study shows that (a) serum and nasal HNE concentrations were significantly higher in RSV bronchiolitis patients than in controls, (b) they did not return to normal after the respiratory symptoms had improved, and (c) they showed no significant correlations with clinical score of severity. The results indicate that neutrophils contribute significantly to airway inflammation in these subjects and HNE levels in serum and nasal lavage may be useful markers of inflammation in acute RSV bronchiolitis. PMID: 17311874 [PubMed - indexed for MEDLINE] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447116 Mon, 01 Jan 2007 05:00:00 +0100 1447116 http://www.medworm.com/index.php?rid=1447115&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311875%26dopt%3DAbstract In conclusion, interphase FISH is feasible using frozen-thawed buffy coat; the technique will be useful for retrospective molecular cytogenetic analysis of hematologic malignancies. PMID: 17311875 [PubMed - indexed for MEDLINE] (Source: Annals of Clinical and Laboratory Science) Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447115 Mon, 01 Jan 2007 05:00:00 +0100 1447115 http://www.medworm.com/index.php?rid=1447114&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311876%26dopt%3DAbstract Authors: Riccioni G, Bucciarelli T, Mancini B, Corradi F, Di Ilio C, Mattei PA, D'Orazio N Cardiovascular disease is the most important adult health problem in wealthy countries, where biological factors such as obesity, hypertension, dyslipidemia, diabetes, inappropriate diet, cigarette smoking, and sedentary life-style have contributed to its dissemination. Research concerning nutritional regimens has shown that persons who consume large amounts of fruit and vegetables have lower incidences of cardiovascular diseases, stroke, and tumors, although the precise mechanisms for this protective effect are elusive. Possible explanations include (a) increased consumption of dietary fiber, (b) reduced consumption of dietary cholesterol and other lipids, and (c) increased intake of the antioxi... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447114 Mon, 01 Jan 2007 05:00:00 +0100 1447114 http://www.medworm.com/index.php?rid=1447113&cid=s_36963_166_f&fid=36963&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17311877%26dopt%3DAbstract Authors: Riccioni G, Barbara M, Bucciarelli T, di Ilio C, D'Orazio N The influence of nutrition on chronic bronchial asthma has an important place in the management of this disease. Evidence suggests that specific inflammatory abnormalities exist in the airways of subjects suffering from mild-to-moderate persistent asthma, in whom an inflammatory state is often associated with increased generation of reactive oxygen species and the damaging effects of free radicals. For this reason oxidant stress may be an important pathogenic factor in the progress of the disease. The role of nutrition in bronchial asthma is related to antioxidant vitamins A, C, and E. By counteracting oxidants and reducing external attacks (bacteria, virus, toxins, xenobiotics) in the lung, antioxidant vitamins modul... Annals of Clinical and Laboratory Science journals http://www.medworm.com/rss/comments.php?id=1447113 Mon, 01 Jan 2007 05:00:00 +0100 1447113