MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Arch Histol Cytol' source. Sat, 10 Oct 2009 19:28:01 +0100 http://www.medworm.com/index.php?rid=2847484&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789408%26dopt%3DAbstract In this study, we transplanted cell pellets of Meckel's chondrocytes into isogenic mouse spleens and analyzed their phenotypic transformation into osteogenic cells using histological and immunohistochemical methods. With the increasing duration of transplantation, chondrocytes were incorporated into splenic tissues and formed a von Kossa-positive calcified matrix containing calcium and phosphoric acid, similar to that of intact bone. Type I, II, and X collagens, and the bone-marker proteins osteocalcin, osteopontin, osteonectin, and bone morphogenetic protein-2 (BMP-2) were immunolocalized in the matrix formed by the transplanted chondrocytes. Osteopontin and osteonectin were detected in the calcified matrix at earlier stages than osteocalcin and BMP-2. Type II collagen was expressed durin... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2847484 Sun, 01 Mar 2009 00:00:00 +0100 2847484 http://www.medworm.com/index.php?rid=2847483&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789409%26dopt%3DAbstract Authors: Jackowiak H, Trzcielińska-Lorych J, Godynicki S The microstructure of lingual papillae on the dorsal surface of the tongue of adult Egyptian fruit bats was examined by light microscopy (LM) and scanning electron microscopy (SEM). This elongated tongue with a rounded apex is approximately 3 cm long -- including the 1.7cm length of the anterior free part of the tongue -- which facilitates considerable freedom of movement. The surface of the tongue has four types of lingual papillae: two types of mechanical papillae -- filiform and conical papillae, and two types of gustatory papillae -- fungiform and vallate papillae. Most numerous are filiform papillae with well developed keratinized processes represented by four morphological subtypes -- small, giant, elongated, and bifi... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2847483 Sun, 01 Mar 2009 00:00:00 +0100 2847483 http://www.medworm.com/index.php?rid=2847482&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789410%26dopt%3DAbstract Authors: Li Y, Ohashi R, Naito M The nerve growth factor-induced gene B-beta (NGFI-Bbeta, Nurr1) is a member of the nuclear receptor superfamily that is expressed predominantly in the central nervous system. We used an antibody against the human NGFI-Bbeta to observe the protein expression in neuronal cells in the retina, cerebral neocortex, and midbrain of humans and rats. To provide further insight into the role of NGFI-Bbeta in the differentiation of neuronal cells, we also examined the expression of NGFI-Bbeta in rat ontogeny. A few cells in the midbrain showed the expression of NGFIBbeta from 12 days of gestation, and NGFI-Bbeta positive cells increased in the neocortex, claustrum, thalamus and hypothalamus in the subsequent fetal days. NGFI-Bbeta-positive cells appeared in the in... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2847482 Sun, 01 Mar 2009 00:00:00 +0100 2847482 http://www.medworm.com/index.php?rid=2847481&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789411%26dopt%3DAbstract Authors: Tatsuzuki A, Ezaki T, Makino Y, Matsuda Y, Ohta H The general sugar expression pattern was studied in 9 normal full-term human placentas by the use of 21 individual lectins in combination with immunohistochemistry for various markers to understand the function of the placenta as the site of feto-maternal interactions. In mature intermediate and terminal villi, the brush border of the syncytiotrophoblast layer strongly expressed GlcNAc (as stained by WGA, S-WGA, DSL lectins) but weakly expressed sialic acid (Mal II, SNA). The cytoplasm of the syncytiotrophoblast layer showed weak expressions of GlcNAc and Gal/GalNAc with granular patterns. The cytotrophoblast layer, as also recognized by PCNA and HAI-1, typically expressed GlcNAc (LEL etc.) and Gal/GalNAc (MAL I). We found that... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2847481 Sun, 01 Mar 2009 00:00:00 +0100 2847481 http://www.medworm.com/index.php?rid=2847480&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789412%26dopt%3DAbstract Authors: Morito A, Kida Y, Suzuki K, Inoue K, Kuroda N, Gomi K, Arai T, Sato T We isolated adherent fibroblastic cells after collagenase and dispase treatment of human dental pulp. When human dental pulp cells (hDPCs) were cultured in the presence of basic fibroblast growth factor (bFGF), the ratio of hDPCs in the S-phase was significantly higher in comparison with incubation without bFGF. The ratio of hDPCs expressing STRO-1 as a marker of stem cell populations increased approximately eightfold in the presence of bFGF as opposed to that in the absence of bFGF. We demonstrated the characterization and distinctiveness of the hDPCs and showed that, when cultured with the medium containing serum and bFGF, they were highly proliferative and capable of differentiating in vitro into osteobla... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2847480 Sun, 01 Mar 2009 00:00:00 +0100 2847480 http://www.medworm.com/index.php?rid=2847479&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19789413%26dopt%3DAbstract Authors: Park SW, Lee HN, Jeon GS, Sim KB, Cho IH, Cho SS Transferrin binding protein (TfBP) is a cytoplasmic glycoprotein that was originally isolated from the chick oviduct. As we previously demonstrated the constitutive expression of TfBP in the avian nervous system, in this study we examined whether TfBP is expressed in the reptilian nervous system. In accordance with previous findings in the chicken, oligodendrocytes were most prominently labeled by antiserum to TfBP. Great variability was observed between different regions of the central nervous system (CNS) in terms of TfBP-labeled oligodendrocyte numbers. In the retina, TfBP was localized specifically in the cells that are morphologically oligodendrocytes and present in the optic nerve and the ganglion cell layer. TfBP staining... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2847479 Sun, 01 Mar 2009 00:00:00 +0100 2847479 http://www.medworm.com/index.php?rid=2654466&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19556691%26dopt%3DAbstract Authors: Masu K, Saino T, Kuroda T, Matsuura M, Russa AD, Ishikita N, Satoh Y 5-hydroxytriptamine (5-HT) is an important transmitter for vessel constriction. The present study was performed to clarify the effect of 5-HT on smooth muscles in large- and small-sized cerebral and testicular arterioles by confocal microscopy, with special reference to intracellular Ca2+ concentration ([Ca2+]i) dynamics. In cerebral vessels, 5-HT induced a [Ca2+]i increase and the contraction of smooth muscle cells in large- and midsized arterioles (external diameters>50 microm) but not in small-sized arterioles. Conspicuous [Ca2+]i changes by 5-HT were especially observed in the portions close to the cerebral arterial circle, and the 5-HT-induced responses were caused by both Ca2+ influx and mobilization... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2654466 Mon, 01 Dec 2008 00:00:00 +0100 2654466 http://www.medworm.com/index.php?rid=2550514&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19556690%26dopt%3DAbstract Authors: Scillitani G, Liquori GE, Mastrodonato M, Ferri D The morphofunctional organization of the exocrine cells in the foregut of the red-eared slider turtle, Trachemys scripta, was investigated by histochemistry (PAS, AB pH1.0 and pH 2.5, HID-AB, Bowie), lectin-histochemistry (WGA, SBA, UEA, ConA, PNA, DBA, sialidase-SBA, sialidase-PNA, Paradoxical ConA), and immunohistochemistry (antipepsin, anti-alpha-H(+), K(+) ATPase) to detect regional differences and verify the existence of an oro-aboral gradient in gastric juice secretion. Observations showed that pharyngeal goblet cells have mucins with terminal residuals of GalNAc and sialic acid. In the oesophagus, sulphomucins in the goblet cells are progressively substituted by sialomucins and no glands are found. Gastric surface cells ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2550514 Mon, 01 Dec 2008 00:00:00 +0100 2550514 http://www.medworm.com/index.php?rid=2550513&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19556691%26dopt%3DAbstract Authors: Masu K, Saino T, Kuroda T, Matsuura M, Russa AD, Ishikita N, Satoh Y 5-hydroxytriptamine (5-HT) is an important transmitter for vessel constriction. The present study was performed to clarify the effect of 5-HT on smooth muscles in large- and small-sized cerebral and testicular arterioles by confocal microscopy, with special reference to intracellular Ca(2+) concentration ([Ca(2+)](i)) dynamics. In cerebral vessels, 5-HT induced a [Ca(2+)](i) increase and the contraction of smooth muscle cells in large- and midsized arterioles (external diameters >50 mum) but not in small-sized arterioles. Conspicuous [Ca(2+)](i) changes by 5-HT were especially observed in the portions close to the cerebral arterial circle, and the 5-HT-induced responses were caused by both Ca(2+) influx an... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2550513 Mon, 01 Dec 2008 00:00:00 +0100 2550513 http://www.medworm.com/index.php?rid=2550512&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19556692%26dopt%3DAbstract Authors: Hashimoto M, Kurose T, Kawamata S To develop an experimental model and evaluate the effects of the magnitude and duration of pressure, the rat abdominal wall (25 x 20 mm) was subjected to compression either by a weight or by two magnets. In the weight compression tests, a steel plate was inserted under anesthesia into the rat peritoneal cavity, and the abdominal wall was compressed in situ between the underlying steel plate and a weight placed on the abdominal wall. This method resulted in moderate changes in the subcutaneous connective tissue and muscle at 100 mmHg (13.3 kPa) for 4 h, while some muscle damage was observed at 50 mmHg (6.7 kPa) for 4 h and at 100 mmHg for 2 or 3 h. In the magnet pinching tests, a magnet was inserted into the peritoneal cavity, and another magne... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2550512 Mon, 01 Dec 2008 00:00:00 +0100 2550512 http://www.medworm.com/index.php?rid=2550511&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19556693%26dopt%3DAbstract Authors: Hashimoto E, Nakakura-Ohshima K, Kenmotsu S, Suzuki H, Nakasone N, Saito C, Harada H, Ohshima H Continuously growing rodent incisors have a special epithelial structure for maintaining adult stem cells that shows a bulbous epithelial protrusion at the apical end and is referred to as an "apical bud". Guinea pig cheek teeth (premolars and molars), also continuously growing teeth, have a complex crown shape consisting of plural cusps. The present study clarifies the existence of apical buds in guinea pig premolars/molars as it examines the relationship between the crown shape and the orientation of the apical buds by micro-computed tomography (mu-CT) and immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU). One premolar and three molar teeth in each side of the maxillae and m... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2550511 Mon, 01 Dec 2008 00:00:00 +0100 2550511 http://www.medworm.com/index.php?rid=2527365&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19359804%26dopt%3DAbstract In this study, we focused on the cellular and subcellular localizations of nonheme ferric (Fe(III)) and ferrous (Fe(II)) iron in the adult female rat brain using light and electron microscopic histochemistry. Although Fe(II) deposition was much less dominant than Fe(III), the brain contained iron in both forms. Among the cellular elements of the brain, oligodendrocytes were numerically the most prominent and heavily iron-storing cells. Pericapillary astrocytes and sporadic microglial cells also showed dense iron accumulation. Large neurons involved in the motor system were relatively strongly iron-positive. Subcellularly, Fe(III) and Fe(II) were mainly localized in lysosomes, and occasionally in the cytosol and mitochondria. Furthermore, capillary endothelial cells had Fe(III)-positive rea... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2527365 Mon, 01 Dec 2008 00:00:00 +0100 2527365 http://www.medworm.com/index.php?rid=2527363&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19359805%26dopt%3DAbstract Authors: Sawada H, Tanaka H, Ono M The tracheal epithelium can be induced to move as a cellular sheet by heterotopic transplantation, which offers the opportunity to observe migrating cells as a group in an in vivo environment. We therefor investigated the ultrastructural characteristics of migrating tracheal epithelial cells with special reference to the moving front using this transplantation. The migrating epithelial cells underwent squamous metaplasia and lost their differentiated characteristics such as cilia or secretory granules. Several unique observations were made concerning the mechanism of mobility: one is that epithelial cells in the front were elongated in a direction perpendicular to the course of movement, different from previous reports in vitro. The second is that lam... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2527363 Mon, 01 Dec 2008 00:00:00 +0100 2527363 http://www.medworm.com/index.php?rid=2527361&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19359806%26dopt%3DAbstract Authors: Saino T, Misaki T, Matsuura M, Shikanai T, Satoh Y Dipyridamole, an inhibitor of adenosine uptake as well as a cGMP phosphodiesterase inhibitor, is commonly used in prophylactic therapy for patients with angina pectoris. However, the effects of dipyridamole on systemic blood vessels, especially on the peripheral vascular system, are not well understood. Therefore, the effect of dipyridamole on ATP-induced arteriole contraction was examined with special reference to intracellular Ca(2+) concentration ([Ca(2+)](i)) using real-time confocal microscopy. In cases of 0.1-10microM range, dipyridamole induced only slight [Ca(2+)](i) decreases in smooth muscle cells of both testicular and cerebral arterioles. However, 100microM dipyridamole induced substantial [Ca(2+)](i) decreases in ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2527361 Mon, 01 Dec 2008 00:00:00 +0100 2527361 http://www.medworm.com/index.php?rid=2527359&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19359807%26dopt%3DAbstract Authors: Russa AD, Ishikita N, Masu K, Akutsu H, Saino T, Satoh Y Regulation of the intracellular calcium ion concentration ([Ca(2+)](i)) is critical, because calcium signaling controls diverse and vital cellular processes such as secretion, proliferation, division, gene transcription, and apoptosis. Store-operated calcium entry (SOCE) is the main mechanism through which non-excitable cells replenish and thus maintain this delicate balance. There is limited evidence which indicates that SOCE may be inhibited during mitosis, and the mechanisms leading to the presumed inhibition has not been elucidated. In the present study, we examined and compared the [Ca(2+)](i) dynamics of COS-7 cells in mitotic and non-mitotic phases with special reference paid to SOCE. Laser scanning confocal micro... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2527359 Mon, 01 Dec 2008 00:00:00 +0100 2527359 http://www.medworm.com/index.php?rid=2527357&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19359808%26dopt%3DAbstract Authors: Hirata K, Kanemaru T, Minohara M, Togo A, Kira J The expression of stress-responsive proteins, such as nestin and a 27-kDa heat-shock protein (HSP27), was immunohistochemically examined in order to demonstrate glial responses in the rat olfactory bulb following sensory deprivation. At 3 days to 1 week after sensory deprivation, numerous nestin-expressing cells appeared within the glomerulus of the olfactory bulb. These cells were regarded as reactive astrocytes since they were immunoreactive for glial fibrillary acidic protein and showed hypertrophic features. The glomeruli, in which nestin-immunoreactive astrocytes were localized, were filled with degenerating terminals of olfactory receptor neurons and migrated microglia. A small population of nestin-immunoreactive cells was... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2527357 Mon, 01 Dec 2008 00:00:00 +0100 2527357 http://www.medworm.com/index.php?rid=2166689&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194037%26dopt%3DAbstract Authors: Muramatsu T, Uekusa T, Masaoka T, Saitoh M, Hashimoto S, Abiko Y, Jung HS, Shimono M We investigated the expression and localization of connexins (CX) 26 and 43 in the rat gingival epithelium. RT-PCR analysis revealed CX26 gene expression in both the upper and lower layers of the gingival epithelium and in the total epithelial layer, whereas CX43 gene expression was limited to the lower layer and the total epithelial layer. Immunoreactivity for CX43 was observed in the membranes of adjacent cells from the basal layer to the middle of the prickle cell layer, while immunoreactivity for CX26 was observed in the granular cell layer and lower part of the squamous cell layer. Merged images revealed the co-localization of CX26 and CX43 in the middle of the prickle cell layer. By immu... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2166689 Wed, 01 Oct 2008 04:00:00 +0100 2166689 http://www.medworm.com/index.php?rid=2166688&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194038%26dopt%3DAbstract This study used 100-mum thick paraffin sections stained by the ER-HR3 antibody to examine the three-dimensional surface morphology of the central macrophages of erythroblastic islets in the splenic red pulp of aged and pregnant mice. The ER-HR3-positive cells were the macrophages located at the center of the erythroblastic islets, and the number per unit of splenic area was almost constant until 30 days of age, thereafter showing a marked decrease. In pregnant females, the ER-HR3-positive macrophage number significantly increased and became approximately eight times higher than the control value. In aged virgin females, the islet macrophages were generally ovoid in cell profile, and shallow cup-shaped dents were formed on their cell surface. However, in pregnant females, the macrophages be... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2166688 Wed, 01 Oct 2008 04:00:00 +0100 2166688 http://www.medworm.com/index.php?rid=2166687&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194039%26dopt%3DAbstract Authors: Ikeda T, Abe K, Kuroda N, Kida Y, Inoue H, Wake K, Morito M, Sato T The inhibition of apoptosis by glycyrrhizin (GL) in hepatic injury induced by injection of lipopolysaccharide (LPS)/D-galactosamine (D-GalN) was examined in the present study. Morphological and biochemical analyses of LPS/D-GalN-induced mouse liver injury revealed that apoptosis occurred exclusively in injured hepatocytes of the centrilobular area. The degree of hepatic injury was associated with a substantial number of hepatocytes undergoing apoptosis. Transaminase levels were significantly increased at 6 to 8 h after the injection of LPS/D-GalN compared with controls. GL inhibited the elevation of serum transaminase levels when it was given to mice at 30 min before the administration of LPS/D-GalN. Morpholog... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2166687 Wed, 01 Oct 2008 04:00:00 +0100 2166687 http://www.medworm.com/index.php?rid=2166686&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194040%26dopt%3DAbstract In conclusion, size-dependent and/or organ-specific responses of arterioles to proteases are due to the heterogeneous localization of PARs. PMID: 19194040 [PubMed - in process] (Source: Arch Histol Cytol) Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2166686 Wed, 01 Oct 2008 04:00:00 +0100 2166686 http://www.medworm.com/index.php?rid=2166685&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194041%26dopt%3DAbstract Authors: Hinenoya N, Naito I, Momota R, Sado Y, Kumagishi K, Ninomiya Y, Ohtsuka A In the present study, we have analyzed the alpha(IV) chain distribution in the subepithelial basement membrane (BM) of the rat pulmonary airway from the bronchi to alveoli. We have furthermore analyzed the alpha(IV) chain distribution in the subepithelial BM of the bronchioalveolar duct junction (BADJ) using alpha(IV) chain specific monoclonal antibodies. Our results show that the BM of the bronchial and bronchiolar epithelium contains [alpha1(IV)](2)alpha2(IV) and [alpha5(IV)](2)alpha6(IV) molecules and confirmed that the alveolar BM consists of [alpha1(IV)](2)alpha2(IV) and alpha3(IV) alpha4(IV)alpha5(IV) molecules. There are also small regions in BADJ consisting of only [alpha1(IV)](2)alpha2(IV) molec... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2166685 Wed, 01 Oct 2008 04:00:00 +0100 2166685 http://www.medworm.com/index.php?rid=2166684&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19194042%26dopt%3DAbstract Authors: Minaguchi J, Oohashi T, Inagawa K, Ohtsuka A, Ninomiya Y The aim of the current study was to investigate the specific accumulation of the Sialyl Lewis X (SLX) liposome in inflammation in the collagen-antibody induced arthritic (CAIA) model mice. The SLX-liposome encapsulating fluorescent substance (Cy5.5 or Cy3) was prepared for this study. The SLX-liposome was administered intravenously via the mouse caudal vein. After 1 to 24 h, the accumulation of SLX-liposome was observed using in vivo fluorescent imaging equipment (eXplore Optix), or the knee joints were removed for histological analysis. The in vivo fluorescent imaging showed that the signal was confined to the inflammatory site in the CAIA mice in an inflammatory dependent manner. The signal intensity was stronger at 24... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=2166684 Wed, 01 Oct 2008 04:00:00 +0100 2166684 http://www.medworm.com/index.php?rid=1926166&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18974599%26dopt%3DAbstract Authors: Ohtani O, Ohtani Y The lymph node comprises a critical crossroad for encounters between antigen presenting cells, antigens from lymph, and lymphocytes recruited into lymph nodes from the blood. The node consists of spaces lined with lymphatic endothelial cells and parenchyma. The former spaces can be divided into the subcapsular sinuses, lymphatic labyrinths in the deep cortex, intermediate sinuses, and medullary sinuses. The sponge-like framework of the node parenchyma is composed of collagen fibers invested with reticular cells. The parenchyma can be divided into the cortex, deep cortex, and medullary cord. Lymphocytes migrate from the node parenchyma into the lymphatic labyrinths in the deep cortex. Close to the labyrinths are high endothelial venules (HEVs), through which ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1926166 Sun, 01 Jun 2008 04:00:00 +0100 1926166 http://www.medworm.com/index.php?rid=1926165&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18974600%26dopt%3DAbstract Authors: Yamamoto T, Suzuki H, Kubo Y, Matsumoto A, Uemura H We investigated the distribution of endothelin A (ET(A)) receptor-like immunoreactivity in the rat kidney using affinity-purified antibodies against amino acid residues 403-417 of the rat ET(A) receptor modified by the multiple antigen peptide complex system. Western blot analysis using the affinity-purified anti-ET(A) antibody detected bands of approximately 47.3 and 64.5 kDa in the rat kidney. By light microscopy, ET(A) receptor-like immunoreactivity was seen in the basal side of the renal tubules and collecting ducts. The most intense immunoreactivity was present in the distal renal tubules and inner medullary collecting ducts. In addition to the basal infoldings, immunoreactive puncta were scattered in the epithelial cell... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1926165 Sun, 01 Jun 2008 04:00:00 +0100 1926165 http://www.medworm.com/index.php?rid=1926164&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18974601%26dopt%3DAbstract Authors: Tsuchiya M, Akiba Y, Takahashi I, Sasano Y, Kashiwazaki J, Tsuchiya S, Watanabe M Root resorption lacunae are principally formed by odontoclasts. While these cells develop from the same origin as osteoclasts, odontoclasts normally have fewer nuclei and a less clear zone compared with osteoclasts. We therefore, hypothesized that odontoclasts possess less differentiation in matrix resorption characteristics than osteoclasts. To test our hypothesis, we compared the TRAP-positive area and the expression patterns of two important proteolytic enzymes, cathepsin K and matrix metalloproteinase-9 (MMP-9), between odontoclasts and osteoclasts. We focused on physiological root resorption in the rat molar, which is a useful experimental model for estimating odontoclasts and osteoclasts. O... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1926164 Sun, 01 Jun 2008 04:00:00 +0100 1926164 http://www.medworm.com/index.php?rid=1926163&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18974602%26dopt%3DAbstract Authors: Iwatsuki H, Meguro R, Asano Y, Odagiri S, Li C, Shoumura K The perfusion-Perls and -Turnbull methods supplemented by diaminobenzidine intensification demonstrated the generation and localization of chelatable Fe (II) which can catalyze the generation of cytotoxic hydroxyl radicals (OH.) during the Fenton reaction in rat kidneys exposed to 40 min ischemia or 40 min-ischemia followed by 60 min-reperfusion. The kidneys exposed to 40 min-ischemia showed Fe (II)-deposits largely localized in the deeper half of the cortex, where the deposits densely filled the tubular cell nuclei, with a small amount of them in the cytoplasm of the proximal convoluted tubules (PCT). Intraluminally protruded or exfoliated tubular cell nuclei were also filled with the deposits. The kidneys subjected t... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1926163 Sun, 01 Jun 2008 04:00:00 +0100 1926163 http://www.medworm.com/index.php?rid=1926162&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18974603%26dopt%3DAbstract In conclusion, preganglionic nerve endings in the adrenal medulla of the golden hamster show differential morphological changes following PX depending on the intracellular part of A cells. PMID: 18974603 [PubMed - in process] (Source: Arch Histol Cytol) Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1926162 Sun, 01 Jun 2008 04:00:00 +0100 1926162 http://www.medworm.com/index.php?rid=1926161&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18974604%26dopt%3DAbstract Authors: Takayasu N, Yoshikawa M, Watanabe M, Tsukamoto H, Suzuki T, Kobayashi H, Noda S D-Serine, an endogenous and obligatory coagonist for the glycine site of the N-methyl-D-aspartate receptor in mammals, is synthesized from L-serine by serine racemase. Serine racemase and D-serine have long been believed to occur predominantly in astrocytes, according to immunohistochemical studies. Recent studies have demonstrated, however, that both the mRNA and protein levels of serine racemase are considerably higher in neurons than in astrocytes in primary cultures of the rat brain and that the mRNA level of serine racemase predominates in neurons of the adult rat brain. Here we report the application of in situ hybridization based on tyramide signal amplification for the detection of serine r... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1926161 Sun, 01 Jun 2008 04:00:00 +0100 1926161 http://www.medworm.com/index.php?rid=1926160&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18974605%26dopt%3DAbstract We examined the expression of dentin matrix protein genes, Col1a1 and dentin sialophosphoprotein (DSPP), by in situ hybridization, and dentin matrix proteins, osteocalcin, osteopontin, and dentin matrix protein 1 (DMP1) as well as an intermediate filament, nestin, by immunohistochemistry to characterize odontoblasts in Tg(Col1a1-Runx2) mice. Results showed Col1a1 expression was down-regulated, DSPP expression was lost, and nestin expression was severely decreased in the odontoblasts of Tg(Col1a1-Runx2) mice. Further, the expressions of osteocalcin, osteopontin, and DMP1 were up-regulated in odontoblasts, although the up-regulation of osteocalcin expression was transient. These findings indicate that Runx2 inhibits the terminal differentiation of odontoblasts, and that Runx2 induces transdi... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1926160 Sun, 01 Jun 2008 04:00:00 +0100 1926160 http://www.medworm.com/index.php?rid=1704226&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18622090%26dopt%3DAbstract Authors: Ohtani O, Ohtani Y The lymphatic system plays important roles in maintaining tissue fluid homeostasis, immune surveillance of the body, and the taking up dietary fat and fat-soluble vitamins A, D, E and K. The lymphatic system is involved in many pathological conditions, including lymphedema, inflammatory diseases, and tumor dissemination. A clear understanding of the organization of the lymphatic vessels in normal conditions would be critically important to develop new treatments for diseases involving the lymphatic vascular system. Therefore, the present paper reviews the organization of the lymphatic vascular system of a variety of organs, including the thyroid gland, lung and pleura, small intestine, cecum and colon in the rat, the diaphragm in the rat, monkey, and human, ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1704226 Thu, 01 May 2008 04:00:00 +0100 1704226 http://www.medworm.com/index.php?rid=1704225&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18622091%26dopt%3DAbstract Authors: Wakabayashi Y, Tsujimura A, Matsuda K, Yoshimura N, Kawata M In the present study, we found that leucocyte function-associated antigen-1 (LFA-1), and integrin (heterodimer complex of CD11a and CD18), which are abundant in immunological synapse, were expressed in developing hippocampal neurons. The expression of LFA-1 in hippocampal neurons was in the period of synaptogenesis, and synaptogenesis was inhibited by the blocking antibodies of anti-CD11a or anti-CD18 in vitro. Since it is known that LFA-1 has an important role in the immunological response, especially in immunological synapse, LFA-1 is considered to have an important role in neuronal synapse and is highly involved in synaptogenesis in the early developmental stage in vitro. In vivo, we also confirmed that CD18 was e... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1704225 Thu, 01 May 2008 04:00:00 +0100 1704225 http://www.medworm.com/index.php?rid=1704224&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18622092%26dopt%3DAbstract In conclusion, this in vitro study showed that decorin is a regulatory and architecturally small leucine-rich repeat proteoglycan in the process of collagen fibril assembly. PMID: 18622092 [PubMed - in process] (Source: Arch Histol Cytol) Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1704224 Thu, 01 May 2008 04:00:00 +0100 1704224 http://www.medworm.com/index.php?rid=1704223&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18622093%26dopt%3DAbstract In conclusion, capillarity was closely correlated with the muscle fiber radius (which represents the perimeter) during growth and atrophy. This indicates that the capillarity is linked to the muscle fiber surface area (which is determined by perimeter and section thickness), in agreement with the essential role of the cell membrane in the transport of materials by simple diffusion or active transport. PMID: 18622093 [PubMed - in process] (Source: Arch Histol Cytol) Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1704223 Thu, 01 May 2008 04:00:00 +0100 1704223 http://www.medworm.com/index.php?rid=1704222&cid=s_30455_171_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18622094%26dopt%3DAbstract Authors: Ueda K, Ichimori Y, Maruyama H, Murakami Y, Fujii M, Honma S, Wakisaka S The present study employed immunohistochemistry for single-stranded DNA (ssDNA) to detect apoptotic cells in taste buds of the rat circumvallate papilla. Double-labeling of ssDNA and markers for each cell type - phospholipase C beta2 (PLCbeta2) and alpha-gustducin for type II cells, neural cell adhesion molecule (NCAM) for type III cells, and Jacalin for type IV cells - was also performed to reveal which types of cells die by apoptosis. We detected approximately 16.8% and 14.0% of ssDNA-immunoreactive nuclei among PLCbeta2-immunoreactive and alpha-gustducinimmunoreactive cells, respectively, but rarely found ssDNA-immunoreactive cells among NCAM-immunoreactive or Jacalin-labeled cells, indicating that typ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1704222 Thu, 01 May 2008 04:00:00 +0100 1704222 http://www.medworm.com/index.php?rid=1395967&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18431027%26dopt%3DAbstract Authors: Hanata K, Yamaguchi N, Yoshikawa K, Mezaki Y, Miura M, Suzuki S, Senoo H, Ishikawa K The basement membrane functions as a barrier against the invasion of cancer cells. It is therefore important to investigate the mechanism of basement membrane degradation by matrix metalloproteinases (MMPs). Previously, cancer cells were long considered to be the major source of MMPs; however, current evidence indicates that most MMPs in cancer tissue are produced by stromal rather than cancer cells. A glycoprotein highly expressed on the cancer-cell membrane, EMMPRIN (extra-cellular matrix metalloproteinase inducer), exhibits the potential role of the MMP inductor in stromal cells. Depending on the cell type, EMMPRIN can stimulate the production of MMP-1, MMP-2, and MMP-3. We here report that... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1395967 Tue, 01 Apr 2008 04:00:00 +0100 1395967 http://www.medworm.com/index.php?rid=1395966&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18431028%26dopt%3DAbstract In this study, the expression of esRAGE in the hippocampal tissues from AD brains compared with control (non-AD) was examined by immunohistochemistry and Western blot analysis. Semiquantitative immunohistochemical analysis of hippocampal tissues using esRAGE-specific antibody revealed significantly decreased immunoreactivities in pyramidal cells in CA1 and CA3 regions of AD compared with non-AD. On the other hand, immunoreactivities of astrocytes for esRAGE significantly increased in those regions. Dentate granule cells and astrocytes showed essentially invariant immunoreactivities between AD and non-AD. Changes in esRAGE immunoreactivity in CA3 neurons and astrocytes were observed from the early pathological stages. Moreover, the esRAGE-immunoreactive bands of AD samples were weaker than ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1395966 Tue, 01 Apr 2008 04:00:00 +0100 1395966 http://www.medworm.com/index.php?rid=1395965&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18431029%26dopt%3DAbstract Authors: Ohkubo Y, Yokosuka H, Kumakura M, Yoshie S Vallate taste buds in the guinea-pig tongue were immunohistochemically investigated with regard to the colocalization of gustducin with calbindin-D28K (=spot 35 protein) and type III inositol triphosphate receptor (IP(3)R-3) in order to characterize gustducin-immunoreactive cells. Individual taste bud cells ranged from totally immunopositive to totally immunonegative for these three molecules. Among the immunoreactive cells, gustducin-immunoreactive cells were divided into two cell populations: one immunopositive and the other immunonegative for calbindin-D28K. Applying our previous data to the present results, the former cells should belong to Type III cells designated by electron microscopy. This finding provides new evidence regard... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1395965 Tue, 01 Apr 2008 04:00:00 +0100 1395965 http://www.medworm.com/index.php?rid=1395964&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18431030%26dopt%3DAbstract Authors: Shibata-Iwasaki R, Dekimoto H, Katsuyama Y, Kikkawa S, Terashima T Carbocyanine fluorescent dye, DiI, is an excellent anterograde/retrograde neural tracer, but its efficacy for the anterograde labeling of neural circuits in the adult brain tends to decrease with ages. The present study shows that an injection of DiI into the motor cortex of the young adult jimpy mutant mice (Plp1(jp)/+) resulted in successful anterograde labeling of corticospinal tract fibers. Furthermore, an injection of Fast Blue into the lumbar spinal cord of the mutant mice resulted in retrograde labeling of layer 5 corticospinal tract neurons within the motor cortex. Since no abnormality except for myelin deficiency is known in the long descending and ascending tracts of jimpy mutant mouse, this mutant is... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1395964 Tue, 01 Apr 2008 04:00:00 +0100 1395964 http://www.medworm.com/index.php?rid=1395963&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18431031%26dopt%3DAbstract Authors: Hida T, Ikeda H, Kametaka S, Akazawa C, Kohsaka S, Ebisu S, Uchiyama Y, Waguri S Three mammalian GGAs (Golgi-localized, gamma-ear-containing, ARF-binding proteins), GGA1, 2, and 3 have been implicated in the sorting of mannose 6-phosphate receptor (MPR). To investigate the distinct roles of GGA2 in lysosomal enzyme transport, we established two stable cell lines that had a reduced expression of GGA2 by RNA interference. The expression levels of GGA2 were approximately 5% of the control levels, whereas those of non-targeted GGA1 and GGA3 were not apparently reduced. The depletion of GGA2 did not cause changes in the overall distribution of GGA1, GGA3, cation-dependent MPR, or cation-independent MPR. However, the cell lines showed increased secretion of a lysosomal enzyme, cathe... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1395963 Tue, 01 Apr 2008 04:00:00 +0100 1395963 http://www.medworm.com/index.php?rid=1395962&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18431032%26dopt%3DAbstract Authors: Sato H, Naito I, Momota R, Naomoto Y, Yamatsuji T, Sado Y, Ninomiya Y, Ohtsuka A We studied distribution patterns of type IV collagen alpha chains in the subepithelial basement membrane (SBM) of the human gastrointestinal tract - the esophagus through the anal canal - by immunofluorescent microscopy using alpha(IV) chain-specific monoclonal antibodies. The alpha1(IV), alpha2(IV), alpha5(IV), and alpha6(IV) chains were found in the SBM throughout the tract, indicating the localization of [alpha1(IV)](2)alpha2(IV) and [alpha5(IV)](2)alpha6(IV) heterotrimeric molecules. The [alpha1(IV)](2)alpha2(IV) molecule was continuously stained, while the [alpha5(IV)](2)alpha6(IV) molecule was weakly stained in gastric glands and small intestinal crypts. In addition, the SBM at the luminal s... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1395962 Tue, 01 Apr 2008 04:00:00 +0100 1395962 http://www.medworm.com/index.php?rid=1257462&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18296822%26dopt%3DAbstract Authors: Toyoshima K, Seta Y, Toyono T, Kataoka S The present study demonstrated for the first time the localizations and patterns of expression of key enzymes for steroidogenesis, cytochrome P450 side-chain-cleavage (P450scc), and P450 aromatase in the taste buds of rat circumvallate papillae, using immunoblot analyses and immunohistochemistry. Immunoblot analyses showed that proteins with a molecular weight close to that of rat adrenal cytochrome P450scc and a molecular weight close to that of rat ovary cytochrome P450 aromatase were present in the rat circumvallate papillae. In immunohistochemistry, antibodies against cytochrome P450scc and P450 aromatase yielded the labelings of a subset of taste bud cells. In the double immunolabeling of P450scc and alpha-gustducin or phospholipas... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1257462 Sat, 01 Dec 2007 05:00:00 +0100 1257462 http://www.medworm.com/index.php?rid=1257461&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18296823%26dopt%3DAbstract Authors: Omura Y Cell proliferation in the pineal organ of the immature rainbow trout Oncorhynchus mykiss was investigated by immunocytochemical demonstration of the proliferating cell nuclear antigen (PCNA) together with photoreceptor-specific opsin. Numerous PCNA-immunoreactive cells were found throughout the pineal end-vesicle and stalk. Two types of PCNA-immuno-reactive cells were distinguished: intensely stained, large ovoid and round cells, and mildly stained, slender fusiform cells. The ovoid type of the former cell was found often in the apical region and the round type in the basal region of the epithelium, while the latter fusiform cells were scattered through the apical and middle regions. Occasionally, close approaches were found between the opsin-immunoreactive photorecept... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1257461 Sat, 01 Dec 2007 05:00:00 +0100 1257461 http://www.medworm.com/index.php?rid=1257460&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18296824%26dopt%3DAbstract Authors: Kuramoto H, Kadowaki M, Sakamoto H, Yuasa K, Todo A, Shirai R The present study was performed to examine the distribution and distinct morphology of the serotonin-containing enterochromaffin (EC) cells in the rat distal colon by immunohistochemical and electron microscopic methods. Serotonin-immunohistochemistry revealed that most of the serotonin-immunoreactive EC cells possessed extended cytoplasmic processes. In particular, the immunoreactive EC cells with long processes located along the body of the crypt were characterized by their bipolar processes comprising one with the terminal swellings extending vertically down to the basal crypt and the other running up along the luminal side - in many cases, with the apical ends reaching the glandular lumen. Moreover, a few EC cel... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1257460 Sat, 01 Dec 2007 05:00:00 +0100 1257460 http://www.medworm.com/index.php?rid=1257459&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18296825%26dopt%3DAbstract Authors: Nakano K, Naito I, Momota R, Sado Y, Hasegawa H, Ninomiya Y, Ohtsuka A The present study aims to identify alpha chains of type IV collagen in the basement membrane of the mouse ovarian follicle and examine their changes during follicular development using immunofluorescence microscopy with specific monoclonal antibodies. The basement membrane of the serous mesothelium enveloping the ovary contained all alpha chains of type IV collagen, alpha1(IV) through alpha6(IV) chains. Primordial follicles showed a distinct immunoreactivity against all six alpha chains in their basement membranes. Immunolabeling for alpha3(IV) and alpha4(IV) chains was almost eliminated in the primary follicles. In basement membranes of secondary and Graafian follicles, the immunofluorescent reaction of al... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1257459 Sat, 01 Dec 2007 05:00:00 +0100 1257459 http://www.medworm.com/index.php?rid=1257458&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18296826%26dopt%3DAbstract Authors: Hasegawa H, Naito I, Nakano K, Momota R, Nishida K, Taguchi T, Sado Y, Ninomiya Y, Ohtsuka A Distributions of type IV collagen alpha chains in the basement membrane (BM) of human skin and its appendages were analyzed by immunofluorescent microscopy using chain-specific monoclonal antibodies. The basement membrane beneath the epidermis contained [alpha1(IV)](2)alpha2(IV) and [alpha5(IV)](2)alpha6(IV) but no alpha3(IV)alpha4(IV)alpha5(IV); this held true for at the eccrine sweat glands and glandular ducts, sebaceous glands, hair follicles, and arrector muscles of hair. The secretary portion of the eccrine sweat glands was rich in [alpha1(IV)](2) alpha2(IV) and had less [alpha5(IV)](2)alpha6(IV), while [alpha5(IV)](2) alpha6(IV) was abundant in the ductal portion. In the subepide... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1257458 Sat, 01 Dec 2007 05:00:00 +0100 1257458 http://www.medworm.com/index.php?rid=1096955&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18079584%26dopt%3DAbstract In conclusion, the Golgi apparatus in hamster A cells shows markedly different ultrastructural features compared with that in rat A cells. PMID: 18079584 [PubMed - in process] (Source: Arch Histol Cytol) Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1096955 Mon, 01 Oct 2007 04:00:00 +0100 1096955 http://www.medworm.com/index.php?rid=1096954&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18079585%26dopt%3DAbstract Authors: Iino S, Horiguchi S, Horiguchi K, Nojyo Y Interstitial cells of Cajal (ICC) are important regulatory cells generating electrical rhythmicity and transducing neural signals in the gastrointestinal musculature. ICC express the proto-oncogene c-kit, a receptor tyrosine kinase, and can be examined morphologically using the c-Kit antibody. The c-kit gene is allelic with the murine white-spotting locus W, and the c-kit mutation (W mutation) affects various aspects of hematopoietic cells, germ cells, melanocytes, mast cells, and ICC. Heterozygous W/W( v) mutant mice lack a specific type of ICC and have been used to reveal its function. To search for a new model that lacks a specific type of ICC, we examined homozygous W( v)/W( v) black-eyed-white mice that are viable with anemia. Res... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1096954 Mon, 01 Oct 2007 04:00:00 +0100 1096954 http://www.medworm.com/index.php?rid=1096953&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18079586%26dopt%3DAbstract Authors: Ahn HJ, Paik SK, Choi JK, Kim HJ, Ahn DK, Cho YS, Kim YS, Moon C, Bae YC The microarchitecture of collagen fibrils in the articular disc of the temporomandibular joint (TMJ) plays an important role in dissipating the mechanical load during jaw movement. However, little information is available on its adaptations to the biomechanical environment during development. To address this issue, we analyzed the diameter of collagen fibrils of the articular disc of the rat TMJ with quantitative ultrastructural analysis during postnatal development. The mean diameter of the collagen fibrils significantly increased and the arrangement of the collagen fiber networks became compact during development. Articular discs of suckling rat pups were composed of thin, uniformly sized collagen fibri... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1096953 Mon, 01 Oct 2007 04:00:00 +0100 1096953 http://www.medworm.com/index.php?rid=1096952&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18079587%26dopt%3DAbstract Authors: Murabayashi H, Kuramoto H, Kawano H, Sasaki M, Kitamura N, Miyakawa K, Tanaka K, Oomori Y The distribution of substance P (SP) immunoreactivity and the colocalization of SP with other bioactive substances in chromaffin cells and nerve fibers were investigated in the rat adrenal gland at the light microscopic level. In the capsule and cortex, SP immunoreactivity was seen in some nerve fibers around blood vessels and in thick nerve bundles passing through the cortex directly into the medulla. In the medulla, the SP immunoreactivity was observed in a small number of chromaffin cells; these SP-immunoreactive chromaffin cells were either phenylethanolamine N-methyltransferase (PNMT) immunoreactive or immunonegative, indicating that they were either adrenaline cells or noradrenaline... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1096952 Mon, 01 Oct 2007 04:00:00 +0100 1096952 http://www.medworm.com/index.php?rid=1096951&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18079588%26dopt%3DAbstract Authors: Yamashita A, Kunimatsu T, Yamamoto T, Yoshida K We investigated cyclooxygenase-2 (Cox-2) immunoreactive cells in the hippocampus and dentate gyrus of Sprague-Dawley rats at 4 h after the induction of normothermic and hypothermic ischemia and reperfusion. Under the normothermic condition, Cox-2 immunoreactive cells showed more intense staining and clearer proximal dendrite configurations as compared with the control animals, whereas the numbers of immunoreactive cells in the hippocampus and dentate gyrus were not remarkably increased. In contrast to the normothermic condition, long-term (pre- and intra-ischemic) and short-term (exclusively intra-ischemic) hypothermic conditions caused a drastic increase in immunoreactive cells in the dentate gyrus. Nearly all granule cells were... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1096951 Mon, 01 Oct 2007 04:00:00 +0100 1096951 http://www.medworm.com/index.php?rid=1096950&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18079589%26dopt%3DAbstract Authors: Kobayashi K, Hoshino K, Homma S, Takagi S, Norita M The thalamic lateral posterior nucleus (LP) of the hooded rat is regarded as a relay nucleus for the transmission of information from visuomotor-related structures such as the superior colliculus, pedunculopontine tegmental nucleus (PPT) and substantia nigra, pars reticulata, to visual cortical areas as well as the striatum. The aim of the present study was to examine the relationships of the thalamo-striatal projection neurons with the LP afferent fibers derived from the PPT, using injections of the anterograde tracer biotinylated dextran amine (BDA) and the retrograde tracer cholera toxin-B (CTB) into the PPT and the striatum, respectively. Findings showed that the location of terminals derived from PPT and LP neurons proje... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=1096950 Mon, 01 Oct 2007 04:00:00 +0100 1096950 http://www.medworm.com/index.php?rid=873550&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17827666%26dopt%3DAbstract Authors: Kitahara K, Sakai Y, Hosaka M, Hira Y, Kakizaki H, Watanabe T To clarify the acute and chronic effects of GnRH agonists on pituitary gonadotropes, changes in the ultrastructure of male rat gonadotropes were examined immunocytochemically and morphometrically after the administration of a one-month depot formulation of the GnRH agonist, leuprorelin. Immediately after the depot administration, the relative amounts of secretory granules drastically decreased in gonadotropes concomitantly with a marked increase in the plasma LH level. After the acute hyperstimulated phase, secretory granules in gonadotropes were gradually restored although the newly synthesized granules were less densely immunolabeled for LHbeta; their relative amounts and sizes were still significantly smaller tha... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=873550 Sun, 01 Jul 2007 04:00:00 +0100 873550 http://www.medworm.com/index.php?rid=873549&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17827667%26dopt%3DAbstract In this study, we clarify the contribution of vitamin A-storing cells to renal fibrosis by focusing on Cygb/ STAP. Adult mice were subjected to unilateral ureteral obstruction (UUO) and kidneys were harvested 1, 3, 7, and 10 days after UUO. Numbers of Cygb/STAP-immunopositive cells as well as Cygb/STAP mRNA 3 days after UUO (UUO day 3 kidney) increased. Vitamin A-autofluorescence was observed in intertubular spaces of controls but gradually declined in a time-dependent manner after UUO. Cygb/STAP+ cells were not completely identical with alpha-smooth muscle actin (alphaSMA)-positive cells in the control or UUO day 7 kidneys. Immunohistochemical analysis for Cygb/STAP and fibulin-2 (Fib), a specific marker for distinguishing MFs from activated HSCs, revealed that the number of Fib+STAP+ cel... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=873549 Sun, 01 Jul 2007 04:00:00 +0100 873549 http://www.medworm.com/index.php?rid=873548&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17827668%26dopt%3DAbstract Authors: Hosoyamada Y, Sakai T The ultrastructure of the rat intestinal interstitium with regard to the mechanical components was analyzed from a functional viewpoint utilizing serial horizontal as well as longitudinal sections through the lamina propria mucosae, including both villi and crypts. The axial smooth muscle cells in the villi (villus-axial SMs) exhibited different configurations at various levels of the wall. They were separated from the voluminous fluid-filled spaces by sheet-like processes of fibroblasts in the upper part of the intravillous interstitium, formed a sheet around the central lymphatics, and were covered by the sheet-like processes of fibroblasts in the lower part of the intravillous interstitium. These villus-axial SMs were poorly developed and associated wi... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=873548 Sun, 01 Jul 2007 04:00:00 +0100 873548 http://www.medworm.com/index.php?rid=873547&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17827669%26dopt%3DAbstract Authors: Suzuki Y, Mizoguchi I, Uchida N Neurotrophic factors are thought to function in the survival and maintenance of the taste buds and nerve fibers innervating them. Laser capture microdissection (LCM) coupled with the reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the mRNA of neurotrophic factors and their receptors in the taste buds of adult mouse circumvallate papillae. Results showed mRNAs of the ciliary neurotrophic factor (CNTF), its receptor (CNTFR), glial cell line-derived neurotrophic factor (GDNF), GDNF family receptors alpha-1 (GFRalpha-1), GFRalpha-2, and RET tyrosine kinase receptor (RET), neurotrophin (NT)3, NT4/5, tyrosine kinase (Trk) C, nerve growth factor (NGF), and TrkA were expressed in the isolated taste buds. Among these neur... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=873547 Sun, 01 Jul 2007 04:00:00 +0100 873547 http://www.medworm.com/index.php?rid=873546&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17827670%26dopt%3DAbstract Authors: Yoshikawa M, Takayasu N, Hashimoto A, Sato Y, Tamaki R, Tsukamoto H, Kobayashi H, Noda S D-serine is an endogenous and obligatory coagonist for the glycine site of the N-methyl-D-aspartate receptor in the mammalian brain. D-serine is synthesized from L-serine by serine racemase; immunohistochemical studies have long been believed to indicate that serine racemase and D-serine occur predominantly in astrocytes. However, we have recently demonstrated in the primary cultures that both the mRNA and protein levels of serine racemase are higher in neurons than in astrocytes. Here we report the application of in situ hybridization based on tyramide signal amplification for the detection of serine racemase mRNA in sections of the adult rat brain. Serine racemase mRNA could be demonstra... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=873546 Sun, 01 Jul 2007 04:00:00 +0100 873546 http://www.medworm.com/index.php?rid=873545&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17827671%26dopt%3DAbstract Authors: Meng FW, Shimoda H, Kajiwara T, Matsuda M, Kato S The intestinal mucosa is vulnerable to an ischemia-reperfusion (I/R) attendant on some bowel diseases and surgery; thus, the restoration of the mucosal integrity is critical to achieving functional recovery of the intestine injured by I/R. In this histochemical study, we investigated the alteration of the central lacteals--which are essential for the transport of fat, tissue fluid, and immune cells in the intestinal mucosa--in the murine jejunum after I/R. The intestine inflicted with I/R demonstrated mucosal injury involving the inflammatory response, with interstitial edema, disruption of the villous tissue, and subsequent tissue regeneration of the villi. The regenerative villous tissue revealed lymphatic regrowth showing pr... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=873545 Sun, 01 Jul 2007 04:00:00 +0100 873545 http://www.medworm.com/index.php?rid=669258&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17558140%26dopt%3DAbstract Authors: Meguro R, Asano Y, Odagiri S, Li C, Iwatsuki H, Shoumura K We reviewed the methods of nonheme-iron histochemistry with special focus on the underlying chemical principles. The term nonheme-iron includes heterogeneous species of iron complexes where iron is more loosely bound to low-molecular weight organic bases and proteins than that of heme (iron-protoporphyrin complex). Nonheme-iron is liberated in dilute acid solutions and available for conventional histochemistry by the Perls and Turnbull and other methods using iron chelators, which depend on the production of insoluble iron compounds. Treatment with strong oxidative agents is required for the liberation of heme-iron, which therefore is not stained by conventional histochemistry. The Perls method most commonly used in la... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=669258 Sun, 17 Jun 2007 02:41:32 +0100 669258 http://www.medworm.com/index.php?rid=669257&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17558141%26dopt%3DAbstract Authors: Barberini F, Makabe S, Franchitto G, Correr S, Relucenti M, Heyn R, Familiari G In order to elucidate the ultrastructural dynamics of endometrium differentiation, uterine samples of fetuses aged 14 to 22 weeks of gestation (WG) were analyzed. Samples were processed for light (LM), transmission (TEM) and field-emission scanning electron microscopy (FE-SEM). Initial stratification of the uterine wall occurred at 14 WG: endometrial, myometrial, and perimetrial primordia were identified. At this age, the endometrial epithelium was simple columnar to pseudostratified and consisted of microvillous cells. Blood capillaries developed mainly in the stroma and between the myometrium and perimetrium primordia. At 18-20 WG the endometrial epithelium became clearly pseudostratified, with a... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=669257 Sun, 17 Jun 2007 02:41:32 +0100 669257 http://www.medworm.com/index.php?rid=669256&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17558142%26dopt%3DAbstract Authors: Daitoku D, Kurose T, Mori E, Hashimoto M, Kawamata S An experimental design was developed for morphometric analysis of the subcutaneous connective tissue after the subcutaneous injection of 0.1 ml of saline or a histamine solution (0.01, 0.1 or 1% histamine dihydrochloride in saline). The subcutaneous connective tissue of 4-week-old rats, originally 170.0 +/- 13.6 mum in thickness, swelled 5.2-fold at 15 min, 3.0-fold at 2 h, and 1.2-fold at 6 h after the injection of saline. The total cross sectional area of both blood and lymphatic vessels increased when compared to that at pre-injection (0.0186 +/- 0.0030 mm(2) in 6-mm-long specimen), 1.4-fold at 15 min, 2.2-fold at 2 h, and 1.1-fold at 6 h post-injection, while the total number of these vessels increased 1.1-fold at 2 h. T... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=669256 Sun, 17 Jun 2007 02:41:32 +0100 669256 http://www.medworm.com/index.php?rid=669255&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17558143%26dopt%3DAbstract Authors: Inaga S, Katsumoto T, Tanaka K, Kameie T, Nakane H, Naguro T This paper introduces an aqueous solution of platinum blue (Pt-blue) as an alternative to uranyl acetate (UA) for staining in transmission electron microscopy (TEM). Pt-blue was prepared from a reaction of cis-dichlorodiamine-platinum (II) (cis-platin) with thymidine. When Pt-blue was dried on a microgrid and observed by TEM it showed a uniform appearance with tiny particles less than 1 nm in diameter. The effect of Pt-blue as an electron stain was then examined not only for positive staining of conventional ultrathin resin sections and counterstaining of post-embedding immuno-electron microscopy but also for negative staining. In ultrathin sections of the rat liver and renal glomerulus, Pt-blue provided good contras... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=669255 Sun, 17 Jun 2007 02:41:32 +0100 669255 http://www.medworm.com/index.php?rid=669254&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17558144%26dopt%3DAbstract Authors: Tanaka Y, Okado H, Terashima T The reeler mouse is an autosomal recessive mutant mouse caused by mutation of the reelin gene and characterized by cerebellar ataxia. To determine whether the distribution pattern of precerebellar nuclei neurons in the brainstem of the reeler mouse changes, we injected a small volume of a replication-defective recombinant adenovirus carrying E. coli beta-galactosidase (lacZ) into the cerebellar cortex of normal and reeler mice. Five days later, the mice were transcardially perfused by a fixative solution. X-gal staining of coronal or sagittal sections of the brainstem revealed that many origins for reticulocerebellar, cuneocerebellar, trigeminocerebellar, and pontocerebellar projections were retrogradely labeled, but only a few olivocerebellar ne... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=669254 Sun, 17 Jun 2007 02:41:32 +0100 669254 http://www.medworm.com/index.php?rid=669253&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17558145%26dopt%3DAbstract Authors: Miyata A, Baba O, Oda T, Ishikawa I, Takano Y C-src deficiency is characterized by osteopetrosis due to impaired bone resorption by hypofunctional osteoclasts and the resultant failure of tooth eruption. In preliminary observations, we frequently encountered erupted molars in c-src deficient mice unlike in other osteopetrotic animals. Here we examine the effects of c-src deficiency on the development of molar teeth with an emphasis on the spatial relation of growing teeth with the surrounding bones. In c-src deficient mice, the magnitude of tooth impaction differed considerably among the types of molars; all maxillary 1st molars were totally impacted deep in the alveolar sockets, whereas most mandibular 1st molars fully erupted into oral cavity. Distribution of osteoclasts in ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=669253 Sun, 17 Jun 2007 02:41:32 +0100 669253 http://www.medworm.com/index.php?rid=411762&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287575%26dopt%3DAbstract Authors: Mistretta CM, Liu HX The fungiform papilla is a gustatory organ that provides a specific tissue residence for taste buds on the anterior tongue. Thus, during development there must be a progressive differentiation to acquire papilla epithelium, then taste cell progenitor epithelium, and finally taste cells within the papilla apex. Arranged in rows, the patterned distribution of fungiform papillae requires molecular regulation not only to induce papillae, but also to suppress papilla formation in the between-papilla tissue. Intact sensory innervation is not required to initiate papilla development or pattern. However, members of several molecular families have now been identified with specific localization in developing papillae. These may participate in papilla development and... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=411762 Fri, 01 Dec 2006 07:00:00 +0100 411762 http://www.medworm.com/index.php?rid=411761&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287576%26dopt%3DAbstract Authors: Miura H, Kusakabe Y, Harada S Mammalian taste buds are maintained through continuous cell renewal so that taste bud cells are constantly generated from progenitor cells throughout life. Taste bud cells are composed of basal cells and elongated cells. Elongated cells are derived from basal cells and contain taste receptor cells (TRC). Morphologically, elongated cells consist of three distinct types of cells: Types I, II and III. In contrast to the remarkable progress in understanding of the molecular basis for taste reception, the mechanisms of taste bud maintenance have remained a major area of inquiry. In this article, we review the expression of regulatory genes in taste buds and their involvement in taste bud cell differentiation. Three major topics include: 1) the Sonic he... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=411761 Fri, 01 Dec 2006 07:00:00 +0100 411761 http://www.medworm.com/index.php?rid=411760&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287577%26dopt%3DAbstract Authors: Shimada S, Ueda T, Ishida Y, Yamamoto T, Ugawa S Taste receptor cells detect gustatory stimuli using a complex arrangement of ion channels, G protein-coupled receptors, and signaling cascades. Sour and salty tastes are detected by ion channels in the rat. Using a combination of homology screening and functional expression approaches, we screened a rat circumvallate papilla cDNA library and identified acid-sensing ion channel-2a (ASIC2a) and ASIC2b as candidates for the rat sour-sensing channels. In situ hybridization and reverse transcription-polymerase chain reaction experiments revealed that ASIC2a and ASIC2b transcripts were localized in taste bud cells. Immunohistochemistry and immunoprecipitation also revealed that both subunits were expressed in a subset of taste cells a... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=411760 Fri, 01 Dec 2006 07:00:00 +0100 411760 http://www.medworm.com/index.php?rid=411759&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287578%26dopt%3DAbstract Authors: Yoshida R, Yasumatsu K, Shigemura N, Ninomiya Y Taste signals are first detected by the taste receptor cells, which are located in taste buds existing in the tongue, soft palate, larynx and epiglottis. Taste receptor cells contact with the chemical compounds in oral cavity through the apical processes which protrude into the taste pore. Interaction between chemical compounds and the taste receptor produces activation of taste receptor cells directly or indirectly. Then the signals are transmitted to gustatory nerve fibers and higher order neurons. A recent study demonstrated many similarities between response properties of taste receptor cells with action potentials and those of the gustatory nerve fibers innervating them, suggesting information derived from receptor cells gen... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=411759 Fri, 01 Dec 2006 07:00:00 +0100 411759 http://www.medworm.com/index.php?rid=411758&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287579%26dopt%3DAbstract Authors: Yamamoto T Taste is unique among the sensory systems in that, besides its recognition of quality, it is innately associated with hedonic aspects of reward and aversion. This review of the literature will show how taste information is conveyed through the central gustatory pathways to the cortical gustatory area and is processed in terms of qualitative and quantitative aspects. Taste information is also sent to the reward system and feeding center via several brain sites including the prefrontal cortex, insular cortex, and amygdala. The reward system contains the ventral tegmental area, nucleus accumbens, and ventral pallidum; it finally sends information to the lateral hypothalamic area, the feeding center. The dopamine system originating from the ventral tegmental area mediat... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=411758 Fri, 01 Dec 2006 07:00:00 +0100 411758 http://www.medworm.com/index.php?rid=411757&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287580%26dopt%3DAbstract Authors: Sharaby AE, Ueda K, Honma S, Wakisaka S We studied the earliest stages of the palate in rat embryos using scanning electron microscopy and immunohistochemistry of growth-associated protein-43 (GAP-43) to investigate the role of nerves in the development of the palatal taste buds. Chronological sequences of the palatal gustatory structures revealed characteristic several stages: 1) At embryonic day 13.5 (E13.5), the palatal shelves were widely separated, and no nerves could be observed in the vicinity of their epithelium which was formed of an undifferentiated single cell layer. 2) At E14, intraepithelial GAP-43-immunoreactive fine nerves were first observed along the medial border of the palatal shelves which became several layers thick but still separate along their entire le... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=411757 Fri, 01 Dec 2006 07:00:00 +0100 411757 http://www.medworm.com/index.php?rid=411756&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287581%26dopt%3DAbstract We examined the expression of galanin and galanin receptors by using a reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and in situ hybridization. RT-PCR analysis showed that mRNA of galanin and GalR2 were detected in the taste bud-containing epithelium of the circumvallate papilla of rats. Immunohistochemical analyses detected galanin was detected in a subset of taste bud cells of the circumvallate papillae. Double-label studies showed that galanin colocalized with alpha-gustducin, NCAM, and PLCbeta2. Our results of double staining with galanin and taste cell markers indicate that galanin-expressing taste cells are type II and type III cells. Taken together with previous studies, these findings show that galanin may function as a taste bud neurotransmitter. ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=411756 Fri, 01 Dec 2006 07:00:00 +0100 411756 http://www.medworm.com/index.php?rid=411755&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287582%26dopt%3DAbstract Authors: Kataoka S, Toyono T, Seta Y, Toyoshima K It has recently become evident that ATP and other extracellular nucleotides could play an important role in signal transductions. ATP mediates excitatory signaling by means of P2X receptors. P2X(3), one of its subtypes, a membrane ligand-gated ion channel, is strongly expressed in peripheral sensory neurons. The aim of the present study was to examine the distribution of nerve fibers expressing P2X(3) receptors in taste buds in the gustatory papillae and soft palate of rats by immunohistochemistry. We found that the fluorescence ATP marker quinacrine stained subsets of taste bud cells. Numerous nerve fibers innervating taste buds were intensely immunostained with the P2X(3) receptor antibody. These nerve fibers ascended among intragemma... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=411755 Fri, 01 Dec 2006 07:00:00 +0100 411755 http://www.medworm.com/index.php?rid=411754&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287583%26dopt%3DAbstract Authors: Ueda K, Ichimori Y, Okada H, Honma S, Wakisaka S Double immunohistochemistry of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins [synaptosomal-associated protein of 25kDa (SNAP-25), syntaxin and vesicle-associated protein-2 (VAMP-2)], and specific cell markers of taste buds cells [alpha-gustducin and phospholipase Cbeta2 (PLCbeta2) for type II cells; neural cell adhesion molecule (NCAM) for type III cells] was applied to gustatory epithelia of the rat circumvallate papillae. All three SNARE proteins were present in some elongated taste buds cells as well as intra-, peri- and subgemmal nerve fibers. Double immunohisotochemistry revealed that nearly all alpha-gustducin and PLCbeta2 immunoreactive cells expressed SNAP-25, syntaxin, an... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=411754 Fri, 01 Dec 2006 07:00:00 +0100 411754 http://www.medworm.com/index.php?rid=451611&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17287582%26dopt%3DAbstract Authors: Kataoka S, Toyono T, Seta Y, Toyoshima K It has recently become evident that ATP and other extracellular nucleotides could play an important role in signal transductions. ATP mediates excitatory signaling by means of P2X receptors. P2X3, one of its subtypes, a membrane ligand-gated ion channel, is strongly expressed in peripheral sensory neurons. The aim of the present study was to examine the distribution of nerve fibers expressing P2X3 receptors in taste buds in the gustatory papillae and soft palate of rats by immunohistochemistry. We found that the fluorescence ATP marker quinacrine stained subsets of taste bud cells. Numerous nerve fibers innervating taste buds were intensely immunostained with the P2X3 receptor antibody. These nerve fibers ascended among intragemmal cell... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=451611 Fri, 01 Dec 2006 05:00:00 +0100 451611 http://www.medworm.com/index.php?rid=234203&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17031021%26dopt%3DAbstract Authors: Li Z, Ohno N, Terada N, Zhou D, Yoshimura A, Ohno S To identify the distribution of endogenous serum proteins in living mouse renal glomeruli under various hemodynamic conditions, we used the periodic acid-Schiff (PAS) and its fluorescence emission as a marker for the glomerular basement membrane (GBM). The immunostaining for collagen type IV was hardly observed without microwave treatment in specimens prepared by an "in vivo cryotechnique". However, PAS staining and its fluorescence emission could be clearly visualized at the GBM with the "in vivo cryotechnique". Under normotensive conditions, immunoreaction products of albumin and immunoglobulin G heavy and light chains (IgG(H+L)) were localized within glomerular capillary loops (GCL) but not colocalized with the PAS fluores... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=234203 Sat, 14 Oct 2006 01:09:02 +0100 234203 http://www.medworm.com/index.php?rid=234202&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17031022%26dopt%3DAbstract Authors: Ishizeki K, Kagiya T, Fujiwara N, Harada H Chick serum (CKS) contains factors that stimulate adipocytes in Meckel's chondrocytes in vitro. In the present study, we analyzed levels of fatty acids in CKS, and further examined whether these had the potential to convert chondrocytes to adipocytes. Phenotypic changes were evaluated by light and electron microscopies, bromodeoxyuridine (BrdU) incorporation, triglyceride assays, and immunocytochemistry. We showed that CKS contained high levels of fatty acids, and a mixed medium containing 5 particular fatty acids inhibited DNA synthesis and the proliferation of chondrocytes as it facilitated their differentiation into adipocytes. The adipocytes produced were sudan-positive multilocular cells that morphologically and histochemically r... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=234202 Sat, 14 Oct 2006 01:09:02 +0100 234202 http://www.medworm.com/index.php?rid=234201&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17031023%26dopt%3DAbstract Authors: Tsuyoshi H, Zenzai K, Okado H, Endo N, Shibata M, Hirano S Morphological reaction of sensory neurons of dorsal root ganglia after peripheral nerve transection was investigated by a nerve tracing method using E. coli lacZ (beta-galactosidase) gene recombinant adenovirus. The sciatic nerve of the rat was transected and inoculated with the gene recombinant adenovirus from the cutting end of nerve fibers. The fixation was accomplished from one to six weeks after inoculation. A whole mount specimen was observed after the reaction in a X-galactocidase substrate. Newly formed sprouting processes of dorsal root ganglion (DRG) cells appeared, all of them sprouting from the primary segment of DRG cells. Developed branches were morphologically categorized in to two types: one was the "li... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=234201 Sat, 14 Oct 2006 01:09:02 +0100 234201 http://www.medworm.com/index.php?rid=234200&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17031024%26dopt%3DAbstract Authors: Asaka M, Ohta K, Muramatsu T, Kurokawa M, Kizaki H, Hashimoto S, Shimono M The present study investigated the expression and distribution of osteopontin in the mouse major salivary glands. The level of osteopontin expression in the mouse submandibular gland was higher (12.7-fold) than that in parotid and sublingual glands at the mRNA level. By Western blot analysis, intense positive bands were seen at the predicted molecular mass (about 55kDa) in all the major salivary glands, while an approximately 30kDa band of osteopontin was detected only in the submandibular gland. Indirect immunofluorescent and immuno-electron microscopy analyses demonstrated the localization of osteopontin in the luminal (apical) membranes of acinar cells in all the salivary glands. Osteopontin was also... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=234200 Sat, 14 Oct 2006 01:09:02 +0100 234200 http://www.medworm.com/index.php?rid=234199&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17031025%26dopt%3DAbstract Authors: Seta Y, Stoick-Cooper CL, Toyono T, Kataoka S, Toyoshima K, Barlow LA Taste buds are multicellular receptor organs embedded in the lingual epithelium of vertebrates. Taste cells within these buds are modified epithelial cells as they lack axons and turnover rapidly throughout life, yet have neuronal properties enabling them to transduce taste stimuli and transmit this information to the nervous system. Taste cells are heterogeneous, comprising types I, II, III and basal cells, and are continually replaced during adult life, raising the question of how these different cells are generated. The molecular mechanisms governing taste cell differentiation are unknown, but the Notch signaling system has been implicated in this process based upon recent gene expression data. Here we in... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=234199 Sat, 14 Oct 2006 01:09:02 +0100 234199 http://www.medworm.com/index.php?rid=121196&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16819148%26dopt%3DAbstract Authors: Takemura M, Sugiyo S, Moritani M, Kobayashi M, Yonehara N Recent advances in the study of pain have revealed somatotopic- and modality-dependent processing and the integration of nociceptive signals in the brain and spinal cord. This review summarizes the uniqueness of the trigeminal sensory nucleus (TSN) in structure and function as it relates to orofacial pain control. The oral nociceptive signal is primarily processed in the rostral TSN above the obex, the nucleus principalis (Vp), and the subnuclei oralis (SpVo) and interpolaris (SpVi), while secondarily processed in the subnucleus caudalis (SpVc). In contrast, the facial nociceptive signal is primarily processed in the SpVc. The neurons projecting to the thalamus are localized mostly in the Vp, moderately in the SpVi, and... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121196 Wed, 14 Jun 2006 06:00:00 +0100 121196 http://www.medworm.com/index.php?rid=121195&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16819149%26dopt%3DAbstract Authors: Anahara R, Yoshida M, Toyama Y, Maekawa M, Kai M, Ishino F, Toshimori K, Mori C Previous reports have revealed that estrogen agonists or anti-androgenic chemicals induce abnormal spermiogenesis in rodents. In the seminiferous epithelium, the apical ectoplasmic specialization (ES) is an actin-based (cell-cell) junctional structure developing between the Sertoli cells and spermatids as is the basal ES also--although it is located between adjoining Sertoli cells. In the apical and basal ES are several adhesion complex proteins that control the spermatid developing process. Cortactin, an actin-binding protein, is one of the ES adhesion proteins, combining with several cell-cell adhesions associating proteins. In the present study, 17beta-estradiol (E2, 1.2 microg/kg), bisphenol A ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121195 Wed, 14 Jun 2006 06:00:00 +0100 121195 http://www.medworm.com/index.php?rid=121194&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16819150%26dopt%3DAbstract Authors: Yasui T, Tsukise A, Miura T, Fukui K, Meyer W Cytochemistry of glycoconjugates in the apocrine glands in the scrotal skin of the horse was studied using cytochemical methods for electron microscopy, particularly lectin cytochemistry. The secretory cells possessed a variable number of secretory vesicles, a well-developed Golgi apparatus, and abundant cisternae of the rough endoplasmic reticulum. Additionally, the basolateral plasma membrane formed numerous interdigitating folds. Glycoconjugates with vicinal diol groupings were present predominantly in the secretory vesicles, the Golgi apparatus, the surface coat of the plasma membrane, and the majority of the intracellular membranes. With lectin cytochemistry, the secretory vesicles of the glandular cells exhibited glycoprotein... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121194 Wed, 14 Jun 2006 06:00:00 +0100 121194 http://www.medworm.com/index.php?rid=121193&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16819151%26dopt%3DAbstract Authors: Suzuki H, Amizuka N, Noda M, Amano O, Maeda T The submandibular gland (SMG) has been regarded as an age-stable organ in spite of reports on its structural changes with aging. Although the klotho gene is involved in aging, little information is available regarding its effects on morphological changes of SMGs. The present study examined the histological and immunohistochemical features of SMGs in klotho-deficient mice--which are well-established aging models--by immunohistochemical and histochemical techniques. Five kinds of cellular markers--against NGF, EGF, Mnand Cu/Zn-SOD, and RITC-conjugated phalloidin--were used for the identification of cell types. In klotho-deficient mice, the SMGs lost their granular ducts and each lobe diminished. The granular duct showed strong immuno... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121193 Wed, 14 Jun 2006 06:00:00 +0100 121193 http://www.medworm.com/index.php?rid=121192&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16819152%26dopt%3DAbstract We reported that one inclusion body existed in almost all neurons of the locus coeruleus. The present study examined whether similar inclusion bodies are present in the Syrian hamster (weight, about 60 g). Paraffin sections stained with the modified Holmes' method dis played numerous small inclusion bodies in the cytoplasm of cells in the locus coeruleus. Epon sections (1 microm thick) stained using toluidine blue were observed under light microscopy, and numerous small inclusion bodies were again observed. Under electron microscopy observation, inclusion bodies (<1 microm in diameter) predominantly comprised small granular materials, similar to those described by previous investigators. Although inclusion bodies were devoid of a limiting membrane, the relation ship to cytoplasmic organ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121192 Wed, 14 Jun 2006 06:00:00 +0100 121192 http://www.medworm.com/index.php?rid=121191&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16819153%26dopt%3DAbstract Authors: Tsuchiya B, Sato Y, Kameya T, Okayasu I, Mukai K E-cadherin, which expressed in various epithelial tissues, is important for the maintenance of normal epithelial phenotypes. However, the distribution of N-cadherin in normal human tissues has not been defined systemically. In the present study, we employed a sensitive, reliable immunohistochemical detection system for N-cadherin on formalin-fixed, paraffin-embedded tissue sections, and succeeded in demonstrating N- and E-cadherin protein expressions and their distribution in normal human tissues. E-cadherin immunoreactivity was detected in all the epithelial tissues examined, except for the adrenal cortical cells and granulosa cells. N-cadherin was selectively expressed on epithelial cells of the thymus, pituitary, pancreas, li... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121191 Wed, 14 Jun 2006 06:00:00 +0100 121191 http://www.medworm.com/index.php?rid=121203&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16609265%26dopt%3DAbstract Authors: Ablimit A, Matsuzaki T, Tajika Y, Aoki T, Hagiwara H, Takata K Aquaporins (AQPs), membrane water channel proteins expressed in various tissues and organs, serve in the transfer of water and small solutes across the membrane. We raised antibodies to AQPs using isoform-specific synthetic peptides and surveyed their expression in the rat nasal olfactory and respiratory mucosae. AQP1, AQP3, AQP4, and AQP5 were detected by immunohistochemical and immunoblotting analyses. AQP1 was expressed in the endothelial cells of blood vessels and the surrounding connective tissue cells in the olfactory and respiratory mucosae. AQP1 may be involved in water transfer across the blood vessel wall. In the olfactory epithelium, no AQP was detected in the olfactory sensory cells. Instead, AQP3 was a... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121203 Tue, 14 Mar 2006 07:00:00 +0100 121203 http://www.medworm.com/index.php?rid=121202&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16609266%26dopt%3DAbstract Authors: Rodella LF, Ricci F, Borsani E, Rezzani R, Stacchiotti A, Mariani C, Bianchi R Aluminium (Al) impairs the glutamate-nitric oxide-cGMP pathway and reduces the number of nitroxidergic neurons in the rat somatosensory cortex. To understand better the effect of the time of exposure, we monitored the effect of aluminium administration on nitroxidergic neurons, identified by NADPH-diaphorase (NADPH-d) or by nitric oxide synthase (NOS) staining, after 0.5, 1, 2, 3, 6 and 12 months of aluminium administration. Since neuropeptide Y (NPY) is known to be colocalised with nitric oxide synthase in cortical neurons, the aim of this work was to study the effects of Al administration on the cortical expression of NADPH-d, nNOS, and NPY. NADPH-d or NOS positive neurons were found scattered in ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121202 Tue, 14 Mar 2006 07:00:00 +0100 121202 http://www.medworm.com/index.php?rid=121201&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16609267%26dopt%3DAbstract Authors: Martinovic S, Borovecki F, Miljavac V, Kisic V, Maticic D, Francetic I, Vukicevic S The requirement of a bone morphogenetic protein for the maintenance and stimulation of an osteoblast phenotype was examined using mouse MC3T3-E1 cell cultures. Cells expressed BMP-4 mRNA, which correlated with the stimulation of the osteoblast phenotype. The addition of a BMP-4 specific antibody reduced bone nodules, suggesting that BMP-4 is required for the osteogenic activity of osteoblasts in an autocrine manner. Exogenously added BMP-7 gradually decreased the expression of BMP-4 with a concurrent stimulation of the osteoblast phenotype. Exogenous BMP-7 can therefore substitute for endogenously produced BMP-4 acting as a paracrine factor on osteoblasts.The addition of 17beta estradiol decrea... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121201 Tue, 14 Mar 2006 07:00:00 +0100 121201 http://www.medworm.com/index.php?rid=121200&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16609268%26dopt%3DAbstract Authors: Walter BA, Valera VA, Takahashi S, Matsuno K, Ushiki T We previously showed histologically that, in the rat, the cerebrospinal fluid drains from the subarachnoid space along the olfactory nerves to the nasal lymphatics and empties into the superficial and deep cervical lymph nodes. The present study was performed to investigate whether these lymph nodes play a role in the immune response of the central nervous system. For this purpose, keyhole limpet hemocyanin conjugated with fluorescein isothiocyanate (KLH-FITC) was administered into the subarachnoid space of the rat brain, and the time-kinetics and location of FITC and anti-FITC antibody forming cells in the cervical lymph nodes were studied histologically and immunohistochemically. FITC fluorescence was detected in superfi... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121200 Tue, 14 Mar 2006 07:00:00 +0100 121200 http://www.medworm.com/index.php?rid=121199&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16609269%26dopt%3DAbstract Authors: Misaki T, Satoh Y, Saino T, Ogawa A Protease-activated receptors (PARs) mediate cellular responses to various proteases in numerous cell types, including smooth muscles and the endothelium of blood vessels. To clarify whether the stimulation of PARs induces responses in smooth muscle cells of cerebral arterioles, intracellular Ca2+([Ca2+]i) dynamics and nitric oxide (NO) production during PARs stimulation were investigated in the rat cerebral arterioles by real-time confocal microscopy, since [Ca2+]i and NO are both key factors in the maintenance of strain in blood vessels. Testicular arterioles were also investigated for comparison. In smooth muscle cells of small cerebral arterioles (< 50 microm in diameter), thrombin and PAR1-activating peptide (AP) induced an increase i... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121199 Tue, 14 Mar 2006 07:00:00 +0100 121199 http://www.medworm.com/index.php?rid=121198&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16609270%26dopt%3DAbstract Authors: Nakatani K, Tanaka H, Ikeda K, Sakabe M, Kadoya H, Seki S, Kaneda K, Nakajima Y In the portal tract of the regenerating liver after partial hepatectomy, vascular and bile ductular remodeling takes place in response to the portal hyperdynamic state and parenchymal hyperplasia. In order to reveal phenotypical changes in the portal fibroblasts, we immunohistochemically investigated neural cell adhesion molecules (NCAM) and alpha smooth muscle actin (alphaSMA) expression and the ultrastructural changes in them during liver regeneration. In the control rat liver, portal fibroblasts were negative for both NCAM and alphaSMA. They became positive for both markers two days after partial hepatectomy, increased in staining intensity, reached a maximum at three to four days, then decrease... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121198 Tue, 14 Mar 2006 07:00:00 +0100 121198 http://www.medworm.com/index.php?rid=121197&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16609271%26dopt%3DAbstract Authors: Hoshi O, Shigeno M, Ushiki T The present study introduces a method for obtaining three-dimensional images of native (i.e., unfixed) chromosomes by atomic force microscopy (AFM) in a liquid. Human metaphase chromosomes were isolated from a human lymphoblast-like cell line, K562, by the hexylene glycol procedure according to Wray and Stubble- field (1970), adsorbed on a silane-coated glass slide, and observed in a dynamic force mode (i.e., intermittent contact mode) of AFM in a hexylene buffer solution. In adequate operating conditions, the shape of chromosomes with paired chromatids was clearly and three-dimensionally observed by AFM. At high magnification, globular or fibrous structures about 50 nm thick could be found on the surface of each chromaid, implying that chromatin f... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121197 Tue, 14 Mar 2006 07:00:00 +0100 121197 http://www.medworm.com/index.php?rid=121219&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477142%26dopt%3DAbstract Authors: De S, Turman JE Krox-20 is a C(2)H(2)-type zinc-finger transcription factor that plays an essential role in hindbrain development. The Krox-20 null mutation results in hindbrain anomalies that result in neonatal death due to respiratory and feeding deficits. Here we review our studies of how the Krox- 20 null mutation impacts the development of motor and sensory systems critical for the production of consummatory behaviors (suckling/chewing). First, we demonstrated that Krox-20 null mutants suffer a selective loss of primary jaw-opening muscles during prenatal development. In vivo and in vitro studies are reviewed that highlight intrinsic defects in mutant jaw-opener muscles that contribute to muscle degeneration. Next we focus on the impact of the mutation on proprioceptive n... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121219 Wed, 14 Dec 2005 07:00:00 +0100 121219 http://www.medworm.com/index.php?rid=121218&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477143%26dopt%3DAbstract Authors: Kohno R, Toyono T, Seta Y, Kataoka S, Yamaguchi K, Toyoshima K Synapses between taste receptor cells and primary sensory afferent fibers transmit the output signal from taste buds to the central nervous system. The synaptic vesicle cycle at the synapses involves vesicle docking, priming, fusion, endocytosis, and recycling. Many kinds of synaptic vesicle proteins participate in synaptic vesicle cycles. One of these, synaptotagmin 1, binds Ca(2+) phospholipids with high affinity and plays a role in Ca(2+) regulated neurotransmitter release in the central and peripheral nervous systems. However, the expression patterns of synaptotagmin 1 in rat taste tissues have not been determined. We therefore examined the expression patterns of synaptotagmin 1 and several cell specific marker... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121218 Wed, 14 Dec 2005 07:00:00 +0100 121218 http://www.medworm.com/index.php?rid=121217&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477144%26dopt%3DAbstract Authors: Taniguchi R, Shi L, Fujii M, Ueda K, Honma S, Wakisaka S Lectin histochemistry of Jacalin (Artocarpus integrifolia) and peanut agglutinin (PNA), specific lectins for galactosyl (beta-1, 3) N-acetylgalactosamine (galactosyl (beta-1, 3) GalNAc), was applied to the gustatory epithelium of the adult rat. In the ordinary lingual epithelium, Jacalin and PNA labeled the cell membrane from the basal to granular cell layer. They also bound membranes of rounded-cells at the basal portion of taste buds, but the number of PNA labeled cells was smaller than that of Jacalin labeled cells. There was no apparent difference in the binding patterns of Jacalin and PNA among the taste buds of the lingual papillae and those of the palatal epithelium. Occasionally, a few spindle-shaped cells were l... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121217 Wed, 14 Dec 2005 07:00:00 +0100 121217 http://www.medworm.com/index.php?rid=121216&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477145%26dopt%3DAbstract Authors: Okumura R, Shima K, Muramatsu T, Nakagawa K, Shimono M, Suzuki T, Magloire H, Shibukawa Y Previous reports have shown the expression of several mechanosensitive ionic channels on the plasma membrane in odontoblasts, which are the cells responsible for dentin formation. The membrane characteristics of odontoblasts imply that they could play critical roles in the mechano-transduction of fluid displacement within dentinal tubules into the electrical cell signals, to carry dentin sensation to the central nervous system. However, the direct ionic mechanism underlying such a dentin nociceptive function remains unclear. In the present study, we investigated the expression of the transient receptor potential vanilloid subfamily member 1 (TRPV1) channel - which essentially contributes ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121216 Wed, 14 Dec 2005 07:00:00 +0100 121216 http://www.medworm.com/index.php?rid=121215&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477146%26dopt%3DAbstract Authors: Kido MA, Ibuki T, Danjo A, Kondo T, Zhang JQ, Yamaza T, Yamashita Y, Higuchi Y, Tanaka T The dentin-pulp complex is a peripheral end-organ supplied by dense sensory nerve fibers. Substance P, a representative neuropeptide widely distributed in the dental pulp, has been reported to play roles in pain transmission and the amplification of inflammation. We analyzed here the expression of the neurokinin 1 (NK1) receptor, preferentially activated by substance P, using immunocytochemistry in rat dental pulp at both the light and electron microscopic levels. Conspicuous NK1 receptor immunoreactivity was found in the odontoblasts; immunolabelings were present at their plasma membrane and endosomal structures, especially in their cytoplasmic processes. Immunoreactions for NK1 receptor ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121215 Wed, 14 Dec 2005 07:00:00 +0100 121215 http://www.medworm.com/index.php?rid=121214&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477147%26dopt%3DAbstract Authors: Maruyama Y, Harada F, Jabbar S, Saito I, Aita M, Kawano Y, Suzuki A, Nozawa-Inoue K, Maeda T Neurotrophin-4/5 (NT-4/5) - a member of the neurotrophic factors - is a ligand for TrkB, which has been reported to be expressed in the mechanoreceptive Ruffini endings of the periodontal ligament. The present study examined developmental changes in the terminal morphology and neural density in homozygous mice with a targeted disruption of the nt-4/5 gene and wild-type mice by immunohistochemistry for protein gene product 9.5 (PGP 9.5), a general neuronal marker, and by quantitative analysis using an image analyzer. Postnatal development of terminal Schwann cells was also investigated by enzymatic histochemistry for non-specific cholinesterase activity (ChE). Furthermore, the immuno-ex... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121214 Wed, 14 Dec 2005 07:00:00 +0100 121214 http://www.medworm.com/index.php?rid=121213&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477148%26dopt%3DAbstract Authors: Shi L, Kodama Y, Atsumi Y, Honma S, Wakisaka S The present study examined whether mechanical stimulation is required for morphological maintenance of the Ruffini endings - primary mechanoreceptors in the periodontal ligament of the rat incisors, using a hypofunctional model by immunohistochemistry for protein gene product 9.5. The periodontal Ruffini endings of adult rats were observed to be restricted to the alveolar half of the lingual ligament where they displayed a dendritic arborization of expanded axon terminals with threadlike microprojections. In the experimental group, the tips of the upper and lower incisors were unilaterally ground to reduce mechanical stimulation of the ligament, i.e. occlusal force. A reduction in the occlusal force induced morphological changes i... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121213 Wed, 14 Dec 2005 07:00:00 +0100 121213 http://www.medworm.com/index.php?rid=121212&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477149%26dopt%3DAbstract Authors: Jin HW, Ichikawa H, Nomura K, Mukae K, Terayama R, Yamaai T, Sugimoto T The systemic administration of capsaicin is known to cause a massive loss of sensory primary neurons in newborn rats. Here we examined the trigeminal ganglion neurons immunohistochemically for the possible induction of activated forms of caspases-9 and -3 following a subcutaneous injection of capsaicin in newborn rats. The DNA fragmentation signal was labeled by a TUNEL method. TUNEL-positive neurons were rare (< 0.5%) at 24 h after injection of the vehicle without capsaicin. After the capsaicin injection, TUNEL-positive neurons began to increase by 12 h, reached a peak at 24 h (11.4%), and returned to the control level by 120 h. Vehicle control levels of caspase- 9-immunoreactive (ir) and caspase-3-ir ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121212 Wed, 14 Dec 2005 07:00:00 +0100 121212 http://www.medworm.com/index.php?rid=121211&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477150%26dopt%3DAbstract Authors: Aita M, Maeda T, Takagi R, Seo K The trigeminal subnucleus caudalis (Vc) is a critical relay site for processing nociceptive afferent input from the orofacial area in addition to its modulation by neuroplastic change. Although an administration of capsaicin in neonates induces a selective destruction of substance P (SP)-immunoreactive nerve fibers, little information is available regarding its detailed effects on the Vc, particularly during postnatal development. The present study examined postnatal changes in the distribution of SP in the Vc and trigeminal ganglion (TG) by immunohistochemical techniques in naïve (NV) and neonatally capsaicin-treated (CP) mice, combined with a quantitative analysis. The neonatal mice received a single subcutaneous injection of capsaicin (50 m... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121211 Wed, 14 Dec 2005 07:00:00 +0100 121211 http://www.medworm.com/index.php?rid=121210&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16477151%26dopt%3DAbstract Authors: Ishihama K, Kogo M, Wakisaka S, Turman JE The prenatal development of neural circuits for rhythmical oral-motor behaviors used for feeding is essential for the survival of the newborn mammal. The N-methyl-D-aspartate (NMDA) receptor plays a critical role in brainstem circuits underlying postnatal oral-motor behaviors. To understand a role for the NMDA receptor in the emergence of sucking behavior we conducted physiological and immunohistochemical experiments using fetal rats. Physiology experiments examined the development of the NMDA dose response of the brainstem circuit responsible for generating rhythmical trigeminal activity by recording trigeminal motor outputs using an in vitro preparation. The high dose of NMDA agonist bath application affected the mean cycle duration ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121210 Wed, 14 Dec 2005 07:00:00 +0100 121210 http://www.medworm.com/index.php?rid=121209&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16505580%26dopt%3DAbstract Authors: Sakai Y, Hosaka M, Hira Y, Watanabe T Although hydrophilic acrylic resins including LR White have been widely utilized as embedding media for immunocytochemical use, the constituents of tissues are often extracted by the resin monomer during the infiltration process of the embedment, resulting in a discernible impairment of the ultrastructure when the tissue is weakly fixed only with aldehydes. To minimize the extraction by the resin monomer, the embedding procedure with LR White resin was reexamined in the present study. Among the treatments tested, a partial dehydration with 70% ethanol containing 2% phosphotungstic acid (PTA) well preserved the ultrastructure of the pituitary tissue without spoiling the antigenicity of LHbeta and other representative markers for the Golgi a... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121209 Wed, 14 Dec 2005 07:00:00 +0100 121209 http://www.medworm.com/index.php?rid=121208&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16505581%26dopt%3DAbstract Authors: Inai T, Sengoku A, Guan X, Hirose E, Iida H, Shibata Y Tight junctions regulate paracellular permeability, create the luminal fluid microenvironment of blood vessels and the digestive tract, and also form the protective barrier in the stratified epithelium including the epidermis. Claudins are the integral membrane proteins at tight junctions and form a multigene family composed of at least 24 members, but knowledge of the subcellular localization of each claudin is still fragmentary. We performed RT-PCR for fifteen claudin species to examine the mRNA expression in various mouse tissues, and focused on investigating the subcellular localization of claudin-10 and -15 by immunofluorescence microscopy in various rat tissues. Neither claudin-10 nor -15 was detected in vascular end... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121208 Wed, 14 Dec 2005 07:00:00 +0100 121208 http://www.medworm.com/index.php?rid=121207&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16505582%26dopt%3DAbstract Authors: Moreels M, Vandenabeele F, Deryck L, Lambrichts I Radial glial cells are transiently bipolar cells in the developing central nervous system, best known for their role in guiding migrating neurons. The aim of the present study was to investigate phenotypic characteristics of these bipolar precursor cells in a mixed glial cell culture system derived from the rat neonatal spinal cord. Morphological characterization was assessed by cell-specific immunocytochemical markers (nestin, vimentin, 3CB2) and transmission electron microscopy. Our study yielded substantial evidence showing that the bipolar cells exhibit immunocytochemical and ultrastructural features of radial glial cells. Immunohistochemistry of the neonatal rat spinal cord using the same cell-specific markers suggested th... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121207 Wed, 14 Dec 2005 07:00:00 +0100 121207 http://www.medworm.com/index.php?rid=121206&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16505583%26dopt%3DAbstract Authors: Suzuki H, Amizuka N, Oda K, Li M, Yoshie H, Ohshima H, Noda M, Maeda T Mice homozygous for klotho gene deletion are well established aging models as they mimic certain aspects of human senescence e.g. osteoporosis. Induced senescence may affect cellular functions and alter the histological properties of the extracellular matrices. The present study examined the histological and ultrastructural features of osteocytes and the surrounding bone matrix in klotho-deficient mice. As expected, osteoblasts showed a flattened shape with a weak immunoreactivity for alkaline phosphatase, and the bone matrix contained many empty osteocytic lacunae. The walls of both normal and empty lacunae were intensely immunopositive for osteopontin and dentin matrix protein-1, but featured an inconsist... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121206 Wed, 14 Dec 2005 07:00:00 +0100 121206 http://www.medworm.com/index.php?rid=121205&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16505584%26dopt%3DAbstract Authors: Tachibana T, Nawa T The role of Merkel cells in type I cutaneous mechanoreceptors remains enigmatic though mechanical transduction or neuromodulation function has been proposed. It has been shown that mammalian Merkel cells express immunohistochemical reactions of met-enkephalin, VIP, substance P, and CGRP, though the reactivity differs between species. If any one of these peptides acts as a transmitter or modulator for Merkel nerve terminals, these structures must have a specific receptor for the substance. We therefore studied the immunohistochemical localization of the above-mentioned neuropeptides and their receptors in Merkel cell-nerve endings in rat whisker pads. Specimens were doubly stained with polyclonal antibodies to neuropeptides and their receptors combined with ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121205 Wed, 14 Dec 2005 07:00:00 +0100 121205 http://www.medworm.com/index.php?rid=121204&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16505585%26dopt%3DAbstract Authors: Takeda M, Suzuki Y, Obara N, Tsunekawa H Neurotrophin-3 (NT3) and neurotrophin-4 (NT4) affect the survival and maintenance of central and peripheral neurons. Using an immunohistochemical method, we examined whether the taste bud cells in the circumvallate papillae of normal mice expressed NT3, NT4, and their respective receptors TrkC and TrkB, and if so, what type of cells in the taste buds expressed them. Double immunostaining for either of them and PGP 9.5, NCAM, or gustducin was used to determine which cell types expressed which neurotrophins and receptors. Normal taste bud cells expressed NT3, NT4, and the TrkB receptor, but not TrkC. The percentage of NT3-immunoreactive cells among all taste bud cells was 89.0%, that of NT4-immunoreactive cells, 58.6%, and that of TrkB-im... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121204 Wed, 14 Dec 2005 07:00:00 +0100 121204 http://www.medworm.com/index.php?rid=121226&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16276021%26dopt%3DAbstract In this study, acid secretion of the parietal cell was stimulated with histamine or inhibited with cimetidine, and the samples were cryofixed by plunge freezing for light microscopy or high-pressure freezing for electron microscopy. As a result, the organization of glandular cells was well-preserved and quite similar to freshly excised rat gastric mucosa for at least 2 h after isolation. Immunohistochemistry of H+/K+-ATPase demonstrated a translocation of H+/K+-ATPase from the cytoplasm to the apical membrane associated with histamine-stimulation. In cimetidine-treated mucosa, most of the parietal cells were morphologically in the resting state, showing numerous tubulovesicles in their cytoplasm. In contrast, histamine-stimulated parietal cells exhibited well-developed intracellular canali... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121226 Wed, 14 Sep 2005 06:00:00 +0100 121226 http://www.medworm.com/index.php?rid=121225&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16276022%26dopt%3DAbstract In this study, energy dispersive X-ray microanalysis and an iron staining method were used to determine the localization of Fe in cells of the equine superficial digital flexor tendon. In inflamed tendons, Fe was detected in tendinocytes as well as macrophages. In contrast, negative or weak reactions were observed in many cells in the normal tendon. Although the accepted theory states that the reticuloendothelial system (macrophages and reticuloendothelial cells) is mainly responsible for the uptake and decomposition of erythrocytes, and tendon cells under conditions of inflammation show a functional ability to take up Fe substances. It has been reported that tendinocytes have multiple functions, including synthesis and secretion of collagen, cytokines, and matrix metalloproteinases. Takin... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121225 Wed, 14 Sep 2005 06:00:00 +0100 121225 http://www.medworm.com/index.php?rid=121224&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16276023%26dopt%3DAbstract Authors: Meguro R, Asano Y, Odagiri S, Li C, Iwatsuki H, Shoumura K We previously developed the highly sensitive perfusion-Perls and -Turnbull methods to visualize nonheme ferric (Fe (III)) and ferrous (Fe (II)) iron, respectively. The present study used these methods to investigate the possible presence of nonheme iron in the redox (ferric/ferrous) state in the noneheme iron store (phagolysosomes and siderosomes) of resident macrophages in the rat. The perfusion-Perls and -Turnbull methods at pH 0.6 supplemented by DAB intensification intensely stained resident macrophages of different tissues and organs of normal and iron-overloaded rats. The perfusion-Turnbull method, which is specific for nonheme Fe (II), partly stained hemosiderin at pH 5.3, but hardly stained it at the physiologi... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121224 Wed, 14 Sep 2005 06:00:00 +0100 121224 http://www.medworm.com/index.php?rid=121223&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16276024%26dopt%3DAbstract Authors: Yoshimura K, Dissanayake UB, Nanayakkara D, Kageyama I, Kobayashi K Oral submucous fibrosis (OSF) is a chronic disease of the oral cavity characterized by an inflammatory reaction followed by severe fibro-elastic changes. The aim of the present study was to investigate the three-dimensional morphological changes in the connective tissue cores (CTCs) of the oral mucosa in OSF. The sample consisted of buccal mucosal biopsies from ten human subjects ranging in age from 40-45 years; five of them were clinically diagnosed as having moderate to severe OSF, and the remaining five served as unaffected controls. Half of each biopsy was formalin-fixed and paraffin-embedded for light microscopy, while the other half was fixed in a Karnovsky's solution, treated with HCl to exfoliate the e... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121223 Wed, 14 Sep 2005 06:00:00 +0100 121223 http://www.medworm.com/index.php?rid=121222&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16276025%26dopt%3DAbstract Authors: Sakairi A, Tsukise A, Meyer W The present study thoroughly examined the localization and characterization of glycoconjugates in the secretory epithelium lining the seminal vesicles of the miniature pig, employing light and electron microscopic histochemical procedures, including lectin methods. The present results showed the epithelial cells and luminal secretions to contain glycoconjugates with abundant neutral saccharides and a small amount of acidic saccharides, containing varying types of terminal sugar residues. At ultrastructural levels, the free surface coat of the plasma membrane was rich in alpha-D-Man, alpha-D-Glc, beta-D-Gal, GlcNAc, and sialic acid. The flocculent contents of the secretory vesicles indicated the localization of alpha-D-Man, alpha-D-Glc, alpha-L-Fuc... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121222 Wed, 14 Sep 2005 06:00:00 +0100 121222 http://www.medworm.com/index.php?rid=121221&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16276026%26dopt%3DAbstract Authors: Arudchelvan Y, Tokuda N, Adachi Y, Sawada T, Fukumoto T To understand the roles of cortical thymic epithelial cells (CTECs) in T-lymphocyte development, we analyzed rat thymi recovering from irradiation (6Gy), at the ultrastructural level. The morphological alterations in the CTECs were most prominent during the third to fifth day of recovery, when proliferating thymocytes were observed in the vicinity of the CTECs. The most striking finding among the alterations in the CTECs after irradiation was a cytoplasmic vacuolization with an increased amount of granular and membranous content. The granular content was observed as loosely aggregated structures or finely dispersed granules and dense bodies. The membranous content appeared in various forms including vesicular, tubular, an... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121221 Wed, 14 Sep 2005 06:00:00 +0100 121221 http://www.medworm.com/index.php?rid=121220&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16276027%26dopt%3DAbstract We examined the expression of claudins that regulate the paracellular permeability of ions, solutes, and water through tight junctions in the rat epididymis. RT-PCR analysis showed that claudin-2 and -10 were expressed in a segment-specific manner among fifteen claudins examined. Immunofluorescence microscopy demonstrated that both claudins showed developmental stage- and segment-specific subcellular localizations in the epididymal epithelium. Claudin-2 was detected principally in the apical junctional region in the initial segment at postnatal day 7 and week 10 but lost its expression in all the distal segments including the caput, corpus, and cauda. Claudin-10 was detected principally in the apical junctional region in all segments on postnatal day 7 but only in the initial segment--espe... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121220 Wed, 14 Sep 2005 06:00:00 +0100 121220 http://www.medworm.com/index.php?rid=121232&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16079455%26dopt%3DAbstract In this study, we have used histochemistry to examine the regeneration process of tissue engineered teeth in order to determine the cell types that give rise to these engineered tooth structures. Porcine third molar tooth buds were dissociated into single-cell suspensions and seeded onto a biodegradable polyglycolic acid polymer scaffold. Following varying periods of growth in rat hosts, the specimens were evaluated by histology and immunohistochemistry. Aggregates of epithelial cells were first observed 4-6 weeks after implantation. These aggregates assumed three different shapes: a natural tooth germ-like shape, a circular shape, or a bilayer-bundle. Based on the structure of the stellate reticulum in the dental epithelium, the circular and bilayer-bundle aggregates could be clearly clas... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121232 Tue, 14 Jun 2005 06:00:00 +0100 121232 http://www.medworm.com/index.php?rid=121231&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16079456%26dopt%3DAbstract Authors: Ren S, Takano H, Abe K To understand the bone resorption process on the basis of the morphology of bone resorption lacunae, the inner surface of parietal bones in juvenile mice was exposed with a treatment of ultrasonic waves or NaOCl treatment and examined by scanning electron microscopy (SEM). The bone resorption lacunae were divided into two types (I and II) according to differences in morphological features of their walls; the wall of type I lacunae was covered with loose collagen fibrils, while that of type II lacunae was smooth with almost no fibrillar structures. Collagen fibrils in type I lacunae treated with ultrasonic waves differed in appearance from those treated with NaOCl; the collagen fibrils were thin and displayed a smooth surface in type I lacunae treated wit... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121231 Tue, 14 Jun 2005 06:00:00 +0100 121231 http://www.medworm.com/index.php?rid=121230&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16079457%26dopt%3DAbstract Authors: Reguzzoni M, Protasoni M, Maserati M, Pressato B, Manelli A, Raspanti M The hybridization site of a DNA probe was detected using a scanning electron microscope (SEM), modifying the standard in situ hybridization (ISH) method. The experiments were performed on human metaphases obtained from lymphocyte cultures of human peripheral blood. The libraries and probes used were: 1-chromosome library for the painting of chromosome 1 (wcp 1), an alphoid centromere-specific probe of chromosome 8 (pZ8.4), and the yeast artificial chromosome (YAC) 964-C10 mapped at band p13 on chromosome 12. These probes were labeled by nick translation with biotin and displayed with a gold-conjugated anti biotin goat antibody. The gold signal was amplified by silver enhancement. The chromatides appeared a... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121230 Tue, 14 Jun 2005 06:00:00 +0100 121230 http://www.medworm.com/index.php?rid=121229&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16079458%26dopt%3DAbstract Authors: Zhang M, Shimojo H, Ehara T, Shigematsu H To better understand the relationship between innervation in the sphincter of Oddi and pancreatobiliary diseases, nerve cells which possess nitric oxide synthase (NOS) and/or vasoactive intestinal polypeptide (VIP) were studied immunohistochemically in the sphincter of Oddi and duodenum of humans. Specimens from autopsies included 11 cases with pancreatobiliary diseases and 7 cases without such diseases. An elaborate nerve network was revealed with an anti-S-100 antibody in the sphincter of Oddi and duodenum of all specimens. In the sphincter of Oddi of the control group, approximately 47% of the myenteric nerve cells were NOS positive, whereas 54% were VIP positive. Of the NOS positive nerve cells, 21% were also VIP positive. In contr... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121229 Tue, 14 Jun 2005 06:00:00 +0100 121229 http://www.medworm.com/index.php?rid=121228&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16079459%26dopt%3DAbstract In conclusion, viable blastocysts presented a normal ZP outer surface ultrastructure, whereas unhealthy blastocysts showed an altered ZP outer surface, comparable to that of immature/atretic oocytes. Such alterations could reflect sub-optimal culture conditions and/or could be related to blastocyst degenerative processes. The blastocyst ZP surface ultrastructure was unaffected by the fertilization technique (cIVF or ICSI). These data suggest that blastocyst survival in vitro is related to ZP ultrastructure maintenance. PMID: 16079459 [PubMed - indexed for MEDLINE] (Source: Arch Histol Cytol) Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121228 Tue, 14 Jun 2005 06:00:00 +0100 121228 http://www.medworm.com/index.php?rid=121227&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16079460%26dopt%3DAbstract Authors: Nakamura H, Nagaoka N, Hirata A, Inoue M, Ozawa H, Yamamoto T Scanning electron microscopy (SEM) was employed to study the effect of calcitonin on the distribution of actin filaments in osteoclasts obtained from rat tibiae. Fluorescent microscopy was also applied to examine calcitonin-induced changes in the distribution of actin filaments, non-muscle myosin, M-Ras, and extracellular signal-regulated kinase (ERK) to clarify the role of ERK in the cytoskeleton of osteoclasts. SEM of control osteoclasts revealed a ring-like structure in the peripheral region. Labeled actin filaments and non-muscle myosin were detected in the peripheral region and exhibited a ring-like pattern. Immunoreactivity indicating M-Ras and ERK was also detected in the vicinity of the actin ring. After cal... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121227 Tue, 14 Jun 2005 06:00:00 +0100 121227 http://www.medworm.com/index.php?rid=121240&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15827374%26dopt%3DAbstract Authors: Hasui K, Murata F We investigated non-specific staining in a catalyzed reporter deposition (CARD) reaction and improved its blocking methods in supersensitive immunohistochemistry, based on our simplified catalyzed signal amplification (CSA) system (Hasui et al. 2002). In the CARD reaction using biotinyl tyramide, non-specific staining could be reduced by pretreatment with a casein solution or 3% bovine serum albumin (BSA)-phosphate buffer saline (PBS) with 0.1% Tween 20. In the CARD reaction using FITC-labeled tyramide, non-specific staining could be blocked by pretreatment with 0.3% BSA-PBS with 0.1% Tween 20 or 3% polyethylene glycol-PBS with 01% Tween 20. Thus, our new simplified CSA system features: 1) destruction of the endogenous peroxidase activity; 2) blocking of the ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121240 Sat, 01 Jan 2005 07:00:00 +0100 121240 http://www.medworm.com/index.php?rid=121239&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15827375%26dopt%3DAbstract Authors: Tachibana T, Endoh M, Fujiwara N, Nawa T Serotonin (5-HT) has been a candidate for neurotransmitters in cutaneous type I mechanoreceptors (i.e., Merkel cell-nerve endings). Although recent electrophysiological studies have suggested the presence of the 5-HT2 and 3 receptors in the Merkel cell-nerve endings, the histological localization of these receptors are obscure. We thus immunohistochemically examined the presence of 5-HT1, 2, 3 receptors in Merkel cell-nerve endings in sinus hair follicles of the rat whisker pad. We also studied the immunohistochemical localization of the 5-HT transporter to confirm the site of 5-HT secretion. For this purpose, we used antibodies for the 5-HT1A, 5-HT1B, 5-HT2A, 5-HT2C and 5-HT3 receptors, and for the 5-HT transporter, as well as antibodi... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121239 Sat, 01 Jan 2005 07:00:00 +0100 121239 http://www.medworm.com/index.php?rid=121238&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15827376%26dopt%3DAbstract Authors: Liu W, Hirata K, Kawabuchi M To clarify the possible role of nitric oxide (NO) induced in primary sensory neurons after peripheral axotomy, NO synthase (NOS) immunohistochemistry was carried out on rat L5 dorsal root ganglia after sciatic nerve ligation. The results were compared with the expression of 27-kDa heat shock protein (HSP27), a neuroprotective molecule. In intact animals, NOS-immunoreactive neurons represented about 2% of all dorsal root ganglion (DRG) neurons, whereas HSP27-immunoreactive neurons comprised about 14%. After sciatic nerve ligation, both neurons increased, in number and immunoreactivity, reaching a maximum at 2 weeks, when NOS- and HSP27-immunoreactive neurons represented about 33 and 66%, respectively. NOS-immunoreactive neurons then remained unchang... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121238 Sat, 01 Jan 2005 07:00:00 +0100 121238 http://www.medworm.com/index.php?rid=121237&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15827377%26dopt%3DAbstract Authors: Saito M, Satoh S, Kojima N, Tada H, Sato M, Suzuki T, Senoo H, Habuchi T Recent studies have suggested that an ischemia/reperfusion (I/R) injury enhances the expression of costimulatory adhesion molecules on the vascular endothelium. In the present study, we investigated the protective effects of resveratrol, a phenolic product, on the renal function and expression of CD86 in rat kidneys with I/R injury. Wistar rats were divided into four groups; 1) an I/R group with right nephrectomy and 1-hour clamping of the left renal pedicle; 2) a vehicle group, I/R plus 10% ethanol (0.1 ml/kg/day) administered by intra-peritoneal injection from day -1 through to 7; 3) a resveratrol group, I/R plus 4 mg/kg/day of resveratrol; and 4) a sham group. Blood samples were obtained via the tail v... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121237 Sat, 01 Jan 2005 07:00:00 +0100 121237 http://www.medworm.com/index.php?rid=121236&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15827378%26dopt%3DAbstract Authors: Horiuchi K, Naito I, Nakano K, Nakatani S, Nishida K, Taguchi T, Ohtsuka A The three-dimensional ultrastructure of the filamentous glycocalyx of the brush border in the mouse small intestine was successfully demonstrated by high resolution scanning electron microscopy (SEM). The specimens were fixed with 2% glutaraldehyde in a 0.1M phosphate buffer (pH 7.4), and rinsed with buffered solutions with differently adjusted pH values (pH 3.0, 7.0 or 11.0). They were then osmicated, dried, spatter-coated with gold (1.0-1.5 nm), and observed under a high resolution SEM. The glycocalyx on the luminal surface of the intestinal villi covered the top of the microvilli of the epithelial cells and were well preserved in the specimens treated with an alkaline buffer (pH 11.0). The glycocalyx... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121236 Sat, 01 Jan 2005 07:00:00 +0100 121236 http://www.medworm.com/index.php?rid=121235&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15827379%26dopt%3DAbstract Authors: Li YN, Sakamoto H, Kawate T, Cheng CX, Li YC, Shimada O, Atsumi S The localization of calbindin-D28K (CB) was studied immunocytochemically in laminae I and II of the dorsal horn and in spinal ganglia in the chicken, and compared with the distribution of substance P (SP) using double immunolabeling. At the light microscopic level, CB immunoreactivity was observed most intensely in the lamina II using the avidin-biotinylated peroxidase complex (ABC) and immunofluorescence methods. At the electron microscopic level using the ABC method, CB immunoreactivity was observed in the following three neuronal elements: 1) the scalloped central terminal with many dense-cored vesicles (DCVs) in the synaptic glomerulus; 2) some vesicle-containing dendrites (VCDs) inside or outside the synapt... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121235 Sat, 01 Jan 2005 07:00:00 +0100 121235 http://www.medworm.com/index.php?rid=121234&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15827380%26dopt%3DAbstract Authors: Lu Z, Dono K, Gotoh K, Shibata M, Koike M, Marubashi S, Miyamoto A, Takeda Y, Nagano H, Umeshita K, Uchiyama Y, Monden M Cold ischemia-warm reperfusion injury of liver grafts has been investigated thoroughly, but its underlying mechanism remains poorly understood. Here we show that autophagy is involved not only during cold preservation but also during warm reperfusion following transplantation. Immunohistochemistry using an antibody against LC3, a microtubule associated protein 1 light chain 3 and a marker of autophagosomes, showed dot-like weak staining in hepatocytes of rat liver grafts during cold preservation. Since University of Wisconsin solution for graft preservation lacks amino acids, the induction of autophagy in hepatocytes was similar to that under starvation cond... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121234 Sat, 01 Jan 2005 07:00:00 +0100 121234 http://www.medworm.com/index.php?rid=121233&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15827381%26dopt%3DAbstract Authors: Fukushi D, Ushiki T The ultrastructure of C-banded human metaphase chromosomes was studied by the combined use of light microscopy and atomic force microscopy (AFM). Light microscopy of the C-banded chromosomes showed that the centromeric regions of all chromosomes except the Y chromosome were positively stained. AFM further revealed that the C-positive region was higher than the C-negative region. The area of the C-positive region was specific depending on each chromosome; it ranged from the centromere to the proximal end of the long arm in chromosome 1, while it was restricted to the centromere in chromosomes 2 and 3. At higher magnification, chromatin fibers about 50 nm thick were clearly shown in the entire length of the chromosomes. In the C-positive region, these chromat... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121233 Sat, 01 Jan 2005 07:00:00 +0100 121233 http://www.medworm.com/index.php?rid=121248&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15781980%26dopt%3DAbstract Authors: Kim HJ, Kang SS, Cho GJ, Choi WS Neurosteroids are synthesized de novo and involved in a variety of physiological functions in the central and peripheral nervous systems. Although the steroidogenic acute regulatory protein (StAR) plays an essential role in the steroidogenesis of peripheral endocrine glands, its presence and role in the brain had been previously questioned because of difficulties in detecting it. However, a number of recent studies have confirmed the presence of StAR in rodent and human brains. Moreover, there is evidence suggesting that StAR plays a role in steroidogenesis in the brain, as it does in peripheral endocrine organs. The present review presents data regarding the presence and role of StAR in brain steroidogenesis, demonstrating the essential charac... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121248 Tue, 14 Dec 2004 07:00:00 +0100 121248 http://www.medworm.com/index.php?rid=121247&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15781981%26dopt%3DAbstract This article reviews the effects of estradiol (E(2)), progesterone (P) and P receptor antagonists (PA) on the rhesus macaque endometrium. Ovariectomized macaques can be treated with implants of estradiol (E(2)) and P to induce precisely controlled, artificial menstrual cycles. During these cycles, treatment with E(2) alone induces an artificial proliferative phase marked by extensive endometrial epithelial cell proliferation and increased expression of stromal and epithelial estrogen receptor (ER) and P receptor (PR). Androgen receptor (AR) is also upregulated by E(2) but is expressed only by the endometrial stroma. Progesterone acts on the E(2) primed endometrium to induce secretory differentiation and causes suppression of epithelial and stromal ER, epithelial PR, and stromal AR in the f... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121247 Tue, 14 Dec 2004 07:00:00 +0100 121247 http://www.medworm.com/index.php?rid=121246&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15781982%26dopt%3DAbstract Authors: Brenner RM, Slayden OD Estradiol (E) and progesterone (P) act on the primate endometrium to induce dramatic changes in the vascular system during the menstrual cycle. These changes include vessel breakdown and bleeding during menses, heightened angiogenesis during the early proliferative phase, and extensive growth of the spiral arteries in the luteal phase of the cycle. Because steroid hormone action is dependent upon the presence of specific nuclear receptors in target tissues, we used immunocytochemistry with receptor-specific monoclonal antibodies to characterize the spatial and temporal expression of estrogen receptor alpha (ERalpha), estrogen receptor beta (ERbeta), progesterone receptor PR and androgen (A) receptor (AR) in the endometrial vessels of rhesus macaques (Mac... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121246 Tue, 14 Dec 2004 07:00:00 +0100 121246 http://www.medworm.com/index.php?rid=121245&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15781983%26dopt%3DAbstract Authors: Cunha GR, Cooke PS, Kurita T Steroid sex hormones (17beta-estradiol, testosterone, dihydrotestosterone, and progesterone) and aryl hydrocarbons such as the dioxins regulate epithelial proliferation and secretory protein production and differentiation in their respective target organs in male and female urogenital tracts and mammary glands. Recent evidence has demonstrated that stromal-epithelial interactions are critical for mediating the effects of these molecules on epithelial cells. Our results have indicated that estradiol, testosterone, progesterone, and dioxin regulate epithelial proliferation (stimulation or inhibition) via paracrine mechanisms requiring the appropriate receptor in the stroma. The androgen receptor (AR), estrogen receptor alpha (ERalpha), progesterone r... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121245 Tue, 14 Dec 2004 07:00:00 +0100 121245 http://www.medworm.com/index.php?rid=121244&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15781984%26dopt%3DAbstract Authors: Ikeda K, Inoue S Estrogen has crucial roles in the proliferation of cancer cells in reproductive organs such as the breast and uterus. Estrogen-stimulated growth requires two estrogen receptors (ERalpha and ERbeta) which are ligand-dependent transcription factors. High expression of ERs is observed in a large population of breast tumors. In addition, the positive expression of ERs correlates with well-differentiated tumors, a favorable prognosis, and responsiveness to an endocrine therapy with anti-estrogen drugs in patients with breast cancer. Transcription activities of ERs can be regulated by interacting proteins such as coactivators and kinases as well as ligand-binding. Moreover, ER isoforms lacking an ability to transactivate are involved in breast cancer. Downstream tar... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121244 Tue, 14 Dec 2004 07:00:00 +0100 121244 http://www.medworm.com/index.php?rid=121243&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15781985%26dopt%3DAbstract Authors: Izumi S, Abe K, Hayashi T, Nakane PK, Koji T The adrenocorticotropic hormone (ACTH) acts on adrenocortical cells and promotes steroidogenesis by specific binding to the ACTH (MC-2) receptor (ACTHR). To gain an insight into ACTH action on local steroidogenic organs, we examined the immunohistochemical expression of ACTHR in rat adrenal glands and placentas during the mid-late gestation period. Antibodies against synthetic ACTHR peptides were raised in rabbits, and Western blot analysis showed that the antibody reacted with specific proteins in the rat adrenal glands and placentas. The peroxidase-labeled antibody method revealed that ACTHR was distributed in the plasma membrane and cytoplasm of the parenchymal cells of the adrenocortical zona fasciculata. In the placenta, ACTHR ... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121243 Tue, 14 Dec 2004 07:00:00 +0100 121243 http://www.medworm.com/index.php?rid=121242&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15781986%26dopt%3DAbstract Authors: Hishikawa Y, Tamaru N, Ejima K, Hayashi T, Koji T Keratinocyte growth factor (KGF), a mesenchymal cell derived paracrine growth factor that regulates normal epithelial cell proliferation, appears to be an essential mediator of steroids in various reproductive organs. The present study was designed to determine the expression and role of KGF and its receptor (KGFR) in human breast carcinoma tissues by immunohistochemistry. We also compared the results with the expression of estrogen receptor alpha(ERalpha), ERbeta, the proliferative activity assessed by the labeling index (LI) for the Ki-67 antigen, apoptotic frequency assessed by terminal dUTP nick end-labeling (TUNEL) index, and the expression of Bcl-2. All of KGF-positive cases were ERalpha- positive (p<0.05), but not tha... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121242 Tue, 14 Dec 2004 07:00:00 +0100 121242 http://www.medworm.com/index.php?rid=121241&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15781987%26dopt%3DAbstract Authors: Kitajima M, Khan KN, Fujishita A, Masuzaki H, Koji T, Ishimaru T The arylhydrocarbon receptor (AhR) is a nuclear transcription factor mediating toxic effects of chemicals such as dioxins. The 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a member of polyhalogenated aromatic hydrocarbons family, exerts a wide-variety of toxic effects in a tissue- and species-specific manner including the reproduction process. Recently, AhR-mediated direct effects of TCDD on a cell-specific interaction with ovarian steroids have been shown. However, information regarding the effects of TCDD on the mouse implantation is limited. We therefore examined the expression and localization of AhR in the pregnant mouse uterus from 4 to 10 days of gestation (day 4 to day 10) using immunohistochemistry to inv... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121241 Tue, 14 Dec 2004 07:00:00 +0100 121241 http://www.medworm.com/index.php?rid=121258&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700535%26dopt%3DAbstract Authors: Kierszenbaum AL, Tres LL A combination of exogenous contractile forces generated by a stack of F-actin-containing hoops embracing the apical region of the elongating spermatid nucleus and an endogenous modulating mechanism dependent on the spermatid-containing acrosome-acroplaxome-manchette complex may play a cooperative role in the shaping of the spermatid head. In addition, the manchette is a key element in the transport of vesicles and macromolecules to the centrosome and developing spermatid tails as well as in nucleocytoplasmic transport. The proposed model of spermatid head shaping is based on: 1) currently known structural and molecular components of the F-actin hoops, the main cytoskeletal element of the Sertoli cell ectoplasmic specializations; 2) the molecular featur... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121258 Sun, 14 Nov 2004 07:00:00 +0100 121258 http://www.medworm.com/index.php?rid=121257&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700536%26dopt%3DAbstract This study shows that GFP was specifically expressed in prespermatogonia, spermatogonia and spermatids that faithfully reproduce the endogenous expression of Oct-4. Histochemical analysis revealed that most of the TRA98-positive gonocytes are also positive for GFP. However, the frequency of GFP expressing cells out of TRA98 expressing cells decreased together with the maturation of gonocytes in the first week after birth. To compare the stem cell activity between GFP-positive and -negative populations, we performed a transplantation of sorted cells into testes from an individual population. Colonization efficiency of germ cells from a GFP-positive population resulted in a 30-fold increase in colonization compared with a GFP-negative population. Since the expression of Oct-4 in prespermtogo... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121257 Sun, 14 Nov 2004 07:00:00 +0100 121257 http://www.medworm.com/index.php?rid=121256&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700537%26dopt%3DAbstract Authors: Ogawa T, Ohmura M, Tamura Y, Kita K, Ohbo K, Suda T, Kubota Y Spermatogonial stem cells (SSCs), having yet to possess decisive markers, can only be detected retrospectively by transplantation assay. It was reported recently that mouse gonocytes collected from DBA/2 and ICR neonates propagated in vitro. This cultured germ cell, named the germline stem cell (GS cell), produced functional sperm to make progeny when transplanted into recipient mouse testes. Here we show that GS cell lines can be established not only from neonatal testes but also from the testis of adult mice. We also confirmed that GS cells once transplanted into a host testis can be recovered to resume in vitro expansion, indicating that they are convertible mutually with SSCs in adult testes. Confocal laser micr... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121256 Sun, 14 Nov 2004 07:00:00 +0100 121256 http://www.medworm.com/index.php?rid=121255&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700538%26dopt%3DAbstract This study also showed that Cnot7-/- mouse germ cells transplanted as donors could colonize in recipient wild mouse testes to develop normal spermatogenesis by spermatogonial transplantation assay, suggesting that the abnormal spermatogenesis observed in the Cnot7-/- testes was induced by the impaired testicular microenvironment rather than a germ cell defect. In the present study, we have carried out reciprocal germ cell transplantation in which wild type germ cells were transplanted as donors into the recipient Cnot7-/- testes to evaluate the recipient microenvironment for supporting the spermatogenesis of donor cells. We noticed that donor cell colonization was less efficient in Cnot7-/- than in Cnot7+/- testes, and that the donor derived spermatids in the recipient Cnot7-/- testes show... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121255 Sun, 14 Nov 2004 07:00:00 +0100 121255 http://www.medworm.com/index.php?rid=121254&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700539%26dopt%3DAbstract Authors: Tres LL, Rosselot C, Kierszenbaum AL Programmed cell death or apoptosis was analyzed in rat Sertoli-spermatogonial cell cocultures prepared from 2-9 day old rats using time-lapse video microscopy, a cell viability fluorescence microscopy assay, immunocytochemical markers, and cell-permeable caspase inhibitory peptides with reversible and irreversible effects. We show that apoptosis can initially affect a single member of a spermatogonial cell cohort and that single non-viable spermatogonial cells can remain conjoined to viable spermatogonial cells. The integrity of the cytoskeletal F-actin network and the presence on Bcl-2 immunoreactivity are valuable markers of spermatogonial cell viability. Apoptotic bodies released into the culture medium are generally eliminated after cul... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121254 Sun, 14 Nov 2004 07:00:00 +0100 121254 http://www.medworm.com/index.php?rid=121253&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700540%26dopt%3DAbstract Authors: Kurihara Y, Watanabe H, Kawaguchi A, Hori T, Mishiro K, Ono M, Sawada H, Uesugi S Mouse Prrp (mPrrp)/DAZAP1 is a mouse ortholog of Xenopus Prrp, which is involved in vegetal pole localization of Vg1 mRNA in oocytes and is highly expressed in the testis. The mouse protein has been reported to be a shuttling protein which localizes in the nucleus of pre-meiotic spermatogenic cells and round spermatids, and shifts its location into the cytoplasm in elongating spermatids, suggesting that mPrrp may be involved in mRNA transport as well as that of the Xenopus ortholog. We reexamined immunohistochemical analyses of mPrrp/DAZAP1 during spermatogenesis utilizing a newly established monoclonal antibody and reconfirmed it to be a shuttling protein. We also carried out new observations th... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121253 Sun, 14 Nov 2004 07:00:00 +0100 121253 http://www.medworm.com/index.php?rid=121252&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700541%26dopt%3DAbstract Authors: Maekawa M, Ito C, Toyama Y, Suzuki-Toyota F, Kimura T, Nakano T, Toshimori K A mouse homologue of Drosophila germ cell-less, mouse germ cell-less-1 (mgcl-1), is highly expressed in the testis. Previous report revealed that the fertility of the mgcl-1(-/-) male mice is reduced significantly as a result of various morphological abnormalities in the sperm (Kimura et al., 2003). To elucidate the function of mgcl-1 in spermatogenesis, the expression of mGCL-1 in the wild-type testis was examined. Immunohistochemical studies demonstrated that mGCL-1 first appeared in the nuclei of the pachytene spermatocytes at stage VI of the seminiferous epithelium, and existed in those of spermatids until step 8 during spermatogenesis. mGCL-1 was not detectable after step 9 spermatids. The testic... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121252 Sun, 14 Nov 2004 07:00:00 +0100 121252 http://www.medworm.com/index.php?rid=121251&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700542%26dopt%3DAbstract This study investigated how GOPC deficient spermatids fail to assemble the peri-nuclear structures in round-headed spermatozoa during spermiogenesis in GOPC knockout mouse testes. In step 1-8 spermatids, Golgi-derived proacrosomal vesicles that are transported to the perinuclear region formed acrosome-like vesicles of various sizes, called pseudoacrosomes. The marginal ring of the acroplaxome, which is generally formed between the descending edge of a developing acrosome and nuclear envelope in a wild spermatid, was poorly formed between the pseudoacrosome and nuclear envelope. In step 9-11 elongating spermatids, a majority of pseudoacrosomes were detached from the nucleus and disappeared from the perinuclear region by spermiation. Concomitantly, several failures occurred on the nucleus, m... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121251 Sun, 14 Nov 2004 07:00:00 +0100 121251 http://www.medworm.com/index.php?rid=121250&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700543%26dopt%3DAbstract Authors: Suzuki-Toyota F, Ito C, Toyama Y, Maekawa M, Yao R, Noda T, Toshimori K Male mice deleting the gene encoding GOPC (Golgi-associated PDZ- and coiled-coil motif-containing protein) are infertile, showing globozoospermia with a coiled tail (Yao et al., 2002). We confirmed how and where tail anomalies were produced in spermatids and epididymal spermatozoa by light and electron microscopy. During spermiogenesis, tail formation occurred normally, but a defect was found at the posterior ring. Thereafter, remarkable sperm tail deformations were induced during epididymal passage. In the proximal caput epidiymidis, the tails remained normal and straight, but most of them coiled around the nucleus in the cauda epididymidis. Coiling is presumed to occur with the migration of the cytoplasm... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121250 Sun, 14 Nov 2004 07:00:00 +0100 121250 http://www.medworm.com/index.php?rid=121249&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15700544%26dopt%3DAbstract Authors: Toyama Y, Suzuki-Toyota F, Maekawa M, Ito C, Toshimori K Either a 20 or 200 microg/kg body weight/injection of bisphenol A (BPA) was subcutaneously administered to adult mice and rats for 6 days, and the effects on the testes were investigated by electron and light microscopy. Abnormalities were observed in the spermatids: acrosomal vesicles, acrosomal caps, acrosomes and nuclei of the spermatids were severely deformed. The ectoplasmic specialization between the Sertoli cell and spermatids were also affected: incomplete specialization, redundant ectopic specialization and aplasia were observed. Rats and mice responded similarly to BPA. There were no dose dependencies between the 20- and 200 microg/kg body weight/injection groups. The ectoplasmic specialization between adjoinin... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121249 Sun, 14 Nov 2004 07:00:00 +0100 121249 http://www.medworm.com/index.php?rid=121266&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15570884%26dopt%3DAbstract Authors: Taniguchi R, Shi L, Honma S, Fujii M, Ueda K, El-Sharaby A, Wakisaka S To understand the development of the gustatory structures necessitates a reliable marker for both immature and mature taste buds. It has been reported that the intragemmal cells within the taste buds of adult rats were bound to Ulex europaeus agglutinin-I (UEA-I), a specific lectin for alpha-linked fucose, but it has not been determined whether immature taste buds, i.e. taste buds without an apparent taste pore, are labeled with UEA-I. The present study was conducted to examine the UEA-I binding pattern during the development of the rat gustatory epithelium. In adult animals, UEA-I bound to the membrane of taste buds in all examined regions of the gustatory epithelium. Within the individual taste buds, UEA-... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121266 Tue, 14 Sep 2004 06:00:00 +0100 121266 http://www.medworm.com/index.php?rid=121265&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15570885%26dopt%3DAbstract Authors: Nishijima K, Atoji Y We investigated the rat laryngeal taste buds and their innervation by electron microscopy and immunohistochemical methods. Taste buds were densely arranged in the surface facing the laryngeal cavity of the epiglottis, the aryepiglottic fold, and the cuneiform process of the arytenoid cartilages. The cells of the buds were classified into types I, II, III, and basal cells, the ultrastucture of which was almost the same as that previously reported in lingual taste buds. The type III cells that had synaptic contacts with nerve fibers were considered to be sensory cells. Immunohistochemical analysis revealed thick calbindin D28k-immunoreactive fibers and thin varicose fibers immunoreactive for calcitonin gene-related peptide or substance P in and around the ta... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121265 Tue, 14 Sep 2004 06:00:00 +0100 121265 http://www.medworm.com/index.php?rid=121264&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15570886%26dopt%3DAbstract Authors: Rezzani R, Rodella L, Tartaglia GM, Paganelli C, Sapelli P, Bianchi R The biocompatibility of dental materials is a topic of increasing importance for dentists. We investigated the effects of alloys for ceramic crowns and removable prostheses on the skin and lymphoid organs of rats. We used three groups of Sprague Dawley rats: group I as the control, with groups II and III implanted with different biomaterials into subcutaneous pockets. After sacrifice, the rat skin around the implant and the lymph nodes was studied. The tissues of implanted rats were morphologically different from the control animals as they showed a number of mast cells that increased 7 days after the implant with both alloys. These cells decreased 14 days after the implant with alloy for ceramic crowns wher... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121264 Tue, 14 Sep 2004 06:00:00 +0100 121264 http://www.medworm.com/index.php?rid=121263&cid=s_30455_170_f&fid=30455&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D15570887%26dopt%3DAbstract Authors: Yasui T, Tsukise A, Meyer W The localization of the epidermal hyaluronan in the digital foot pads of the North American raccoon (Procyon lotor) was examined by light and electron microscopic histochemical methods. The thick epidermis contained five epidermal layers composed of typical keratinocytes. In the stratum basale and stratum spinosum, hyaluronic acid was clearly detectable at cytoplasmic processes, in close vicinity to the surface coat of the plasma membrane, and/or in the intercellular space between the basal and spinous cells. The results suggest that epidermal hyaluronan that fills the intercellular space may particularly control water maintenance in the epidermis of the raccoon digital pads to guarantee the optimal mechanoreceptive function of the very specific and... Arch Histol Cytol journals http://www.medworm.com/rss/comments.php?id=121263 Tue, 14 Sep 2004 06:00:00 +0100 121263