MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Archives of Dermatological Research' source. Tue, 16 Mar 2010 14:29:20 +0100 http://www.medworm.com/index.php?rid=3360544&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj543211026700727%2F Abstract  Constant exposure to a wide variety of microbial pathogens represents a major challenge for our skin. Antimicrobial peptides (AMPs) are mediators of cutaneous innate immunity and protect primarily against microbial infections. Cathelicidins were among the first AMPs identified in human skin and recent evidence suggests that they exert a dual role in innate immune defense: At first, due to their antimicrobial activity they kill pathogens directly. In addition, these peptides initiate a potent host response to infection resulting in cytokine release, inflammation and a cellular response. Disturbed cathelicidin expression and function was observed in several common inflammatory skin diseases, such as psoriasis where cathelicidin peptide converts inert self-DNA and se... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3360544 Thu, 11 Mar 2010 02:40:07 +0100 3360544 http://www.medworm.com/index.php?rid=3355887&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F33807g7412863068%2F The objective of this study was to investigate angiogenesis, VEGF and mast cell in vitiligo lesions. Specimens of 30 patients' biopsies, from lesional and perilesional nondepigmented skin were stained for mast cells, CD34 and VEGF. The evaluation was made by image analysis and the measured variables were statistically analyzed. A significantly increased number of CD34 and VEGF positive vessels and mast cells were detected in the centre of the lesion than in the periphery (p < 0.0001, p < 0.0001 and p = 0.001). There was a positive correlation of CD34, VEGF and mast cell number between the centre and the periphery of the lesions (r = 0.877, p < 0.0001; r = 0.946, p < 0.0001 and r = 0.863, p ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3355887 Wed, 10 Mar 2010 15:58:26 +0100 3355887 http://www.medworm.com/index.php?rid=3355889&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj7611677960w0468%2F In this report, we identified a novel mutation of ATP2A2 gene in a Chinese family with DD. It is a novel heterozygous nucleotide G → T transition at position 2,282 in exon 15 of the ATP2A2 gene. Our study expands the database on the ATP2A2 gene mutations in DD. Content Type Journal ArticleCategory Short CommunicationDOI 10.1007/s00403-010-1042-7Authors Jun Song, The Fourth Affiliated Hospital of Soochow University Department of Dermatology, Wuxi No. 4 People’s Hospital Wuxi 214062 Jiangsu ChinaMing Li, Shanghai Jiaotong University School of Medicine Department of Dermatology, Xinhua Hospital 1665, Kongjiang Road Shanghai 200092 ChinaLi-Jia Yang, Nanjing Medical University Department of Dermatology, Affiliated Wuxi No. 2 Hospital Wuxi 214002 Jiangsu ChinaGuo-Long Zhang... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3355889 Wed, 10 Mar 2010 15:58:25 +0100 3355889 http://www.medworm.com/index.php?rid=3355888&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft416p753u40r2702%2F Abstract  Psychological factors, such as stress, anxiety, depression, as well as immuno-inflammatory processes may influence blood rheology. On the other hand, these factors may be involved in triggering, aggravating or maintenance of urticaria symptoms. The present study was designed to compare blood rheological properties of patients with chronic urticaria with positive response to autologous serum skin test (ASST) and negative response to ASST. The study comprised 15 female patients with chronic urticaria showing positive response to ASST, 14 female patients with chronic urticaria showing negative response to ASST and 15 healthy subjects. Rheological properties of erythrocytes, such as deformability and aggregation, were measured. Overall blood viscosity measurements wer... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3355888 Wed, 10 Mar 2010 15:58:25 +0100 3355888 http://www.medworm.com/index.php?rid=3355890&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2256t874w7l13473%2F The objective of the study was to characterize serum imiquimod and metabolite pharmacokinetics. Nineteen subjects with actinic keratoses applied two packets of imiquimod 3.75% cream (18.75 mg imiquimod total) once daily for 21 days to a treatment area approximately 200 cm2 in size on the face and/or balding scalp. Blood samples were obtained prior to application of doses 1, 7, 14 and 21, and at selected timepoints after application of doses 1 and 21. After multiple dosing (day 21) serum imiquimod mean C max was 0.323 (standard deviation 0.159) ng/mL, mean AUC(0-24) 5.974 (3.088) ng h/mL, and mean T½ 29.3 (17.0) h. Steady-state was achieved by day 14; multiple dose accumulation ratios were 2.8 based on imiquimod C max and 3.9 based on AUC. Serum concentrations of i... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3355890 Wed, 10 Mar 2010 15:58:24 +0100 3355890 http://www.medworm.com/index.php?rid=3315502&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa5075un121xn7x53%2F Abstract  Dyschromatosis symmetrica hereditaria (DSH) is a rare autosomal dominant cutaneous disorder characterized by a mixture of hyperpigmented and hypopigmented macules of various sizes on the extremities and caused by the mutations of adenosine deaminase acting on RNA1 (ADAR1) gene. We screened 14 unrelated families or sporadic cases for mutation in the full coding sequence of this gene. Eight novel heterozygous mutations of ADAR1 and four known mutations were identified, including four missense mutations (p.R26K, p.Y1192D, p.R916Q, p.R1155W), six frameshift mutations (p.N205fsX217, p.V211fsX217, p.V404fsX417, p.I914fsX927, p.L1053fsX1076, p.L1070fs1092), and two nonsense mutations (p.R474X, p.R1096X). Interestingly, we failed to detect any mutations of ADAR1 in one fam... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3315502 Thu, 25 Feb 2010 20:27:50 +0100 3315502 http://www.medworm.com/index.php?rid=3289180&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm6m09q487507l7k7%2F Content Type Journal ArticleCategory Letter to the EditorDOI 10.1007/s00403-010-1036-5Authors Masahiro Hayashi, Yamagata University School of Medicine Department of Dermatology Iida-Nishi 2-2-2 Yamagata 990-9585 JapanTamio Suzuki, Yamagata University School of Medicine Department of Dermatology Iida-Nishi 2-2-2 Yamagata 990-9585 Japan Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3289180 Thu, 18 Feb 2010 17:54:20 +0100 3289180 http://www.medworm.com/index.php?rid=3285297&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fvm8m781853w13547%2F Content Type Journal ArticleCategory ErratumDOI 10.1007/s00403-010-1038-3Authors Fu-Xi Wang, Shenzhen Second People’s Hospital Department of Dermatology 3002 Sungang West Road 518039 Shenzhen Guangdong ChinaLi-Jia Yang, Nanjing Medical University Department of Dermatology, Affiliated Wuxi No. 2 Hospital 214002 Wuxi Jiangsu ChinaMing Li, Nanjing Medical University Department of Dermatology, Affiliated Wuxi No. 2 Hospital 214002 Wuxi Jiangsu ChinaShu-Lin Zhang, Shenzhen Second People’s Hospital Department of Dermatology 3002 Sungang West Road 518039 Shenzhen Guangdong ChinaXiao-Hong Zhu, Nanjing Medical University Department of Dermatology, Affiliated Wuxi No. 2 Hospital 214002 Wuxi Jiangsu China Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3285297 Wed, 17 Feb 2010 06:55:39 +0100 3285297 http://www.medworm.com/index.php?rid=3270465&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn575658647022553%2F Abstract  Recently, the important role of T helper 17 (Th17) cells in psoriasis has been clarified; however, the role of IL-17F produced by Th17 cells is still not fully understood. IL-6 exhibits multiple biologic functions, such as regulation of immunological responses including those in psoriatic reactions. Therefore, we examined the production of IL-6 protein in normal human epidermal keratinocytes (NHEKs) stimulated by IL-17F, TNF-α, IL-17A, and IL-17A in combination with TNF-α, and PBS control. We then examined the expression of IL-6 mRNA in mouse skin after intradermal injection of IL-17F. Finally, IL-17F expression in skin biopsy specimens from psoriasis patients was examined by immunohistochemistry. The results showed that IL-17F induced production of IL-6 in NHEKs... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3270465 Thu, 11 Feb 2010 11:47:44 +0100 3270465 http://www.medworm.com/index.php?rid=3256129&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2703k774327773p4%2F Content Type Journal ArticleCategory Letter to the EditorDOI 10.1007/s00403-010-1031-xAuthors Pablo Coto-Segura, Hospital Central Universitario de Asturias Dermatology II Department Oviedo Asturias SpainEliecer Coto, Hospital Central Universitario de Asturias Molecular Genetics Department Oviedo Asturias SpainVictoria Alvarez, Hospital Central Universitario de Asturias Molecular Genetics Department Oviedo Asturias SpainJorge Santos-Juanes, Hospital Central Universitario de Asturias Dermatology II Department Oviedo Asturias Spain Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3256129 Sat, 06 Feb 2010 17:59:44 +0100 3256129 http://www.medworm.com/index.php?rid=3256128&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa1x0233x1r11t852%2F Abstract  Antiphospholipid antibodies, such as anti-β2-glycoprotein I (β2GPI), are present in multibacillary leprosy (MB) patients; however, MB patients do not usually present with antiphospholipid antibody syndrome (APS), which is characterized by thromboembolic phenomena (TEP). Rare cases of TEP occur in leprosy patients, but the physiopathology of this condition remains unclear. In this case–control study, we examined whether single-nucleotide polymorphisms (SNPs) of the β2GPI gene contributed to the risk of leprosy and APS co-morbidity. SNPs Ser88Asn, Leu247Val, Cys306Gly and Trp316Ser were identified in 113 Brazilian leprosy patients. Additionally, anti-β2GPI antibodies and plasma concentrations of β2GPI were quantified. The Ser88Asn, Cys306Gly and Trp316Ser SNP... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3256128 Sat, 06 Feb 2010 17:59:44 +0100 3256128 http://www.medworm.com/index.php?rid=3256127&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft3044787r06r360v%2F Abstract  Mutations in CDH3 gene, encoding P-cadherin, are responsible for hypotrichosis with juvenile macular dystrophy (HJMD), which is a rare autosomal recessive disorder. The HJMD is characterized by congenital sparse hair on scalp and progressive severe degenerative changes of the retinal macula which leads to variable degrees of blindness. The present study reports a large consanguineous Pakistani family with six individuals affected with HJMD. Genotyping using polymorphic microsatellite markers showed linkage of the family to CDH3 gene on chromosome 16q22.1. Sequence analysis of the CDH3 gene revealed a novel splice site mutation (c.IVS10–1 G → A) in intron 10, which leads to skipping of exon 11 and probably synthesizing a non-functional premature trunca... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3256127 Sat, 06 Feb 2010 17:59:44 +0100 3256127 http://www.medworm.com/index.php?rid=3241137&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx000h524655621x7%2F Abstract  Systemic lupus erythematosus (SLE) is an autoimmune disease influenced by genetic and environmental factors. Recently, single nucleotide polymorphisms (SNPs) in the region of B lymphoid tyrosine kinase (BLK) have been shown to be associated with SLE in Caucasian population. In this paper, we genotyped SNP rs2248932 in 1,396 SLE patients of Chinese Han and 4,362 ethnically matched control subjects by using the Sequenom MassArray system. We confirmed that SNP rs2248932 in BLK gene was significantly associated with SLE (P = 1.41 × 10−8 for the allele frequency, Odds ratio [OR] = 0.74, 95% confidence interval (CI): 0.66–0.82).The association of BLK in Chinese SLE patients was consistent with a dominant model (P = 8.88 �... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3241137 Wed, 03 Feb 2010 17:55:41 +0100 3241137 http://www.medworm.com/index.php?rid=3241136&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F777722j607718008%2F Abstract  Staphylococcal skin infections are quite common in human patients. These infections often clear spontaneously, but may also progress locally and/or disseminate to cause serious and sometimes fatal deep infections. The present studies were undertaken to examine the clearance phase of experimental cutaneous Staphylococcus aureus infections in a mouse model system. Previous work in this system has shown that staphylococci applied to the skin rapidly disseminate to the spleen and kidney. In the present experiments the bacteria were found to persist at the skin infection site at a time (8 days after inoculation) when they had disappeared from the spleen and kidney. Examination of the infected skin at earlier times revealed rapid (within 6 h) invasion into the ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3241136 Wed, 03 Feb 2010 17:55:41 +0100 3241136 http://www.medworm.com/index.php?rid=3241138&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6q7rx4735771852k%2F Abstract  Keloid scarring, also known as keloid disease (KD), is a common, abnormally raised fibroproliferative cutaneous lesion that can occur following even minor skin trauma. The aetiopathogenesis of KD has remained an enigma todate compounded by an ill-defined clinical management. There is strong evidence suggesting a genetic susceptibility in individuals affected by KD, including familial heritability, common occurrence in twins and high prevalence in certain ethnic populations. This review aims to address the genetic aspects of KD that have been described in present literature that include inheritance patterns, linkage studies, case–control association studies, whole genome gene expression microarray studies and gene pathways that were significant in KD. In addition... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3241138 Wed, 03 Feb 2010 17:55:39 +0100 3241138 http://www.medworm.com/index.php?rid=3224007&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr66437x0l6821007%2F In this study, we analyzed the complexity of the peripheral blood T cell repertoire with a sensitive b-variable (BV) complementarity-determining region 3 (CDR3) spectratyping analysis and flow cytometry in three-stage IV CTCL/Sezary syndrome patients who achieved complete clinical remission after therapy. The T cell repertoire of peripheral blood T cells before treatment was profoundly abnormal across multiple BV subfamilies. Following treatment, CDR3 spectratype patterns showed dramatic restoration of normal diversity and complexity. However, absolute CD4 counts across multiple BV families remained low for many months, even after identifiable circulating malignant T cell populations were eliminated. These data suggest that the diversity of the T cell repertoire can be recovered afte... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3224007 Thu, 28 Jan 2010 17:59:53 +0100 3224007 http://www.medworm.com/index.php?rid=3205972&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh60537447758t675%2F In conclusion, despite several study limitations, the findings indicate that PGP 9.5 positive nerve fibres could be involved in the pathogenesis of HS. Both regarding the profiles and the cells, further studies remain to show if these differences are primary events, or secondary to e g chronic inflammation, which is considered a major issue of HS. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-010-1028-5Authors Karin Sartorius, Karolinska Institutet, Karolinska University Hospital Huddinge Section of Dermatology, Department of Medicine Stockholm SwedenLennart Emtestam, Karolinska Institutet, Karolinska University Hospital Huddinge Section of Dermatology, Department of Medicine Stockholm SwedenJan Lapins, Karolinska Institutet, Karolinska University Hospital H... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3205972 Fri, 22 Jan 2010 03:00:35 +0100 3205972 http://www.medworm.com/index.php?rid=3168800&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr3867316l2006q14%2F Content Type Journal ArticleCategory Letter to the EditorDOI 10.1007/s00403-009-1027-6Authors Qingqiang Xu, Xi’an Jiaotong University Department of Dermatology, Second Hospital 157 Xiwu Road 710004 Xi’an Shaanxi People’s Republic of ChinaShengxiang Xiao, Xi’an Jiaotong University Department of Dermatology, Second Hospital 157 Xiwu Road 710004 Xi’an Shaanxi People’s Republic of ChinaJia Huo, Xi’an Jiaotong University Department of Dermatology, Second Hospital 157 Xiwu Road 710004 Xi’an Shaanxi People’s Republic of ChinaYingying Dong, Xi’an Jiaotong University Department of Dermatology, Second Hospital 157 Xiwu Road 710004 Xi’an Shaanxi People’s Republic of ChinaJianwen Ren, Xi’an Jiaotong University Department of Dermatology, Second Hospital 157 Xiwu Road 71000... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3168800 Tue, 12 Jan 2010 18:18:04 +0100 3168800 http://www.medworm.com/index.php?rid=3160868&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa6148t322q53lv42%2F In conclusion, if the children with psoriasis show increased ASO titer, the physician should pay attention to the worsening of the psoriasis. Furthermore, early treatment of streptococcal infections might be beneficial in childhood psoriasis. Content Type Journal ArticleCategory Short CommunicationDOI 10.1007/s00403-009-1025-8Authors Sue Kyung Kim, Ajou University School of Medicine Department of Dermatology 5 Wonchon-Dong, Yeongtong-Gu Suwon 443-721 South KoreaHee Young Kang, Ajou University School of Medicine Department of Dermatology 5 Wonchon-Dong, Yeongtong-Gu Suwon 443-721 South KoreaYou Chan Kim, Ajou University School of Medicine Department of Dermatology 5 Wonchon-Dong, Yeongtong-Gu Suwon 443-721 South KoreaEun-So Lee, Ajou University School of Medicine Department of Dermat... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3160868 Fri, 08 Jan 2010 21:37:27 +0100 3160868 http://www.medworm.com/index.php?rid=3160869&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2w12773146816730%2F Abstract  A treatment preparation composed of purified Leishmania (L) antigenic fractions (AS210) induced linear delayed type hypersensitivity (DTH) reactions over a 1–40 μg dose range, in guinea pigs. When a DBA-1 mouse collagen induced arthritis (CIA) model was used to compare AS210 treatment against: a polyvalent vaccine (AS110-1), a monovalent vaccine (AS110-2) and placebo, the AS210 treated mice had the least amount of forepaw inflammation and the lowest mean arthritis scores (MAS). When MAS for day(s) 1–40 were analyzed using one way ANOVA, statistically significant (P < 0.05) differences were seen for the following study groups: PBS versus Dexamethasone and PBS versus AS210. Subsequently, the ANOVA analysis results were corroborated by the Mann�... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3160869 Fri, 08 Jan 2010 21:37:26 +0100 3160869 http://www.medworm.com/index.php?rid=3154451&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb68xq353l5774185%2F Abstract  Immunoadsorption (IA), also termed immunoapheresis, has been established as effective and specific tool advantageous to plasmapheresis to remove immunoglobulin and immune complexes and in cytapheresis, immune cells from the circulation. IA was successfully used in various autoantibody-mediated diseases, e.g. acquired hemophilia, myasthenia gravis, dilated cardiomyopathy, and Guillain–Barré syndrome. In dermatology, IA has been applied as an effective adjuvant treatment for autoimmune bullous diseases. Autoimmune blistering disorders are a heterogeneous group of diseases that are associated with autoantibodies to desmosomal (pemphigus group) and basal membrane zone proteins (pemphigoid group, epidermolysis bullosa acquisita). Because the pathogenic relevance of ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3154451 Mon, 04 Jan 2010 18:17:08 +0100 3154451 http://www.medworm.com/index.php?rid=3154452&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F36818pnu77536113%2F Abstract  Sjögren–Larsson syndrome is a genetic disease characterized by ichthyosis, mental retardation, spasticity and mutations in the ALDH3A2 gene coding for fatty aldehyde dehydrogenase, an enzyme necessary for oxidation of fatty aldehydes and fatty alcohols. We investigated the cutaneous abnormalities in 9 patients with Sjögren–Larsson syndrome to better understand how the enzymatic deficiency results in epidermal dysfunction. Histochemical staining for aldehyde oxidizing activity was profoundly reduced in the epidermis. Colloidal lanthanum perfusion studies showed abnormal movement of tracer into the extracellular spaces of the stratum corneum consistent with a leaky water barrier. The barrier defect could be attributed to the presence of abnormal lamellar bodie... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3154452 Mon, 04 Jan 2010 18:17:07 +0100 3154452 http://www.medworm.com/index.php?rid=3134398&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg73p3kk31410613l%2F This study suggests that DHT may not only be involved in sebum production but also in production of proinflammatory cytokines in acne. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-009-1019-6Authors Weon Ju Lee, Kyungpook National University School of Medicine Department of Dermatology 200, Dongduk-ro, Jung-gu Daegu Republic of KoreaHong Dae Jung, Kyungpook National University School of Medicine Department of Dermatology 200, Dongduk-ro, Jung-gu Daegu Republic of KoreaSeong Geun Chi, Kyungpook National University School of Medicine Department of Dermatology 200, Dongduk-ro, Jung-gu Daegu Republic of KoreaByung Soo Kim, Kyungpook National University School of Medicine Department of Dermatology 200, Dongduk-ro, Jung-gu Daegu Republic of KoreaSeok-Jong Lee, Kyung... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3134398 Wed, 30 Dec 2009 16:44:40 +0100 3134398 http://www.medworm.com/index.php?rid=3134399&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy0g480860308702r%2F In this study, acute chemical irritations were performed on the forearm with application of a 10% sodium lauryl sulphate (SLS) aqueous solution under occlusion for 3 h. Then, glycerol aqueous solutions from 1 to 10% were applied under occlusion for 3 h. After elimination of moist excess consecutive to occlusive condition, in ambient air for 15 and 30 min, skin barrier function was investigated by dual measurement of skin hydration and transepidermal water loss (TEWL). Treatments with SLS solution under occlusion significantly increased TEWL and decreased skin hydration as assessed by capacitance measurements. The SLS irritant property was raised by the occlusion and the water barrier function as well as water content appeared impaired. Recovery with glycerol at low dos... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3134399 Wed, 30 Dec 2009 16:44:38 +0100 3134399 http://www.medworm.com/index.php?rid=3130879&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr32546qwv91034l2%2F Abstract  Platelet activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is known to be present in excess in psoriatic skin, but its exact role is uncertain. In the present study we demonstrate for the first time the role of group VI PLA2 in PAF-induced arachidonic acid release in highly differentiated human keratinocytes. The group IVα PLA2 also participates in the release, while secretory PLA2s play a minor role. Two anti-inflammatory synthetic fatty acids, tetradecylthioacetic acid and tetradecylselenoacetic acid, are shown to interfere with signalling events upstream of group IVα PLA2 activation. In summary, our major novel finding is the involvement of the arachidonyl non-selective group VI PLA2 in PAF-induced inflammatory responses. Content Type Jou... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3130879 Tue, 29 Dec 2009 17:55:31 +0100 3130879 http://www.medworm.com/index.php?rid=3127517&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F51x79x563x334741%2F Abstract  The epidermis is the outermost protection of the organism. As so, defence program has to be initiated in stress situation in order to protect keratinocytes. The EGF receptor (EGFR) controls cell proliferation and migration in keratinocytes, being a major regulator of keratinocyte homeostasis within the epidermis. The EGFR is known to be internalized without addition of ligand under the control of p38 MAPK during stress conditions in HeLa cells, but also following lipid rafts disruption in keratinocytes. This could represent an alternative internalization process that removes the EGFR from cell surface. Here, we investigated whether other stress conditions such as scratch wounding keratinocyte monolayer or incubation with a sensitizer chemical (i.e. DNFB), could al... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3127517 Mon, 28 Dec 2009 19:13:48 +0100 3127517 http://www.medworm.com/index.php?rid=3122773&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy615577774032041%2F In conclusion, our results suggest that a therapeutic approach based on the human skin-derived MSCs may have a positive effect in wound healing conditions, through their ability to provide VEGF and NO to the damaged area. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-009-1018-7Authors Eleonora Salvolini, Polytechnic University of Marche Department of Molecular Pathology and Innovative Therapies, Histology Section Via Tronto 10/A 60020 Ancona ItalyGuendalina Lucarini, Polytechnic University of Marche Department of Molecular Pathology and Innovative Therapies, Histology Section Via Tronto 10/A 60020 Ancona ItalyAntonio Zizzi, Polytechnic University of Marche Department of Molecular Pathology and Innovative Therapies, Histology Section Via Tronto 10/A 60020 Ancon... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3122773 Fri, 25 Dec 2009 00:17:40 +0100 3122773 http://www.medworm.com/index.php?rid=3122774&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp8472p2l28w0321h%2F Content Type Journal ArticleCategory Letter to the EditorDOI 10.1007/s00403-009-1016-9Authors Viroj Wiwanitkit, Wiwanitkit House, Bangkhae Bangkok 10160 Thailand Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3122774 Fri, 25 Dec 2009 00:17:39 +0100 3122774 http://www.medworm.com/index.php?rid=3122775&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft311724784857t31%2F In this study, we ascertained a Spanish family demonstrating the autosomal recessive form of HED. Affected individuals in the family showed the characteristic features of HED, including fine and sparse scalp hair, sparse eyebrows and eyelashes, periorbital hyperpigmentation, prominent lips, hypodontia and conical teeth, reduced sweating, and dry and thin skin. Sequence analysis of the EDAR gene revealed a novel compound heterozygous mutation [c.52-2A>G; c.212G>A (p.Cys71Tyr)]. Our finding extends the body of evidence that supports the significance of the EDAR signalling pathway in the ectodermal morphogenesis. Content Type Journal ArticleCategory Short CommunicationDOI 10.1007/s00403-009-1013-zAuthors M. R. Moya-Quiles, Virgen de la Arrixaca University Hospital Biochemistry ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3122775 Thu, 24 Dec 2009 19:56:40 +0100 3122775 http://www.medworm.com/index.php?rid=3121277&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft722855182222q04%2F Content Type Journal ArticleCategory Letter to the EditorDOI 10.1007/s00403-009-1015-xAuthors Hong-Liang Zhang, The First Hospital of Jilin University Department of Neurology Changchun ChinaJiang Wu, The First Hospital of Jilin University Department of Neurology Changchun China Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3121277 Wed, 23 Dec 2009 22:21:06 +0100 3121277 http://www.medworm.com/index.php?rid=3121278&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fat1113r1p7762500%2F Abstract  Physiological wound repair is a highly regulated, complex process, which leads to formation of new tissue after injury. However, the healing process is not perfect and healing impairments can occur. Delayed healing and formation of chronic wounds has been linked to the excessive production of proteolytic enzymes leading to reduced amounts of growth factors and successive destruction of the extracellular matrix. It has been implied that there is an alteration in the normal control mechanisms regulating the levels of these enzymes. The study presented provides data on the concentration of proteases and cytokines in wound fluid from chronic when compared with acute wounds. Levels of proteases such as PMN elastase, matrix metalloproteinases-2 (MMP-2), and MMP-13 were ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3121278 Wed, 23 Dec 2009 22:21:03 +0100 3121278 http://www.medworm.com/index.php?rid=3121279&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh7554766k8k85177%2F Abstract  Development of vitiligo-like hypopigmentary lesions associated with topical imiquimod has been reported. We hypothesized that mode of action of imiquimod in melanocytes may include triggering of apoptosis resulted in loss of cells, which may be a possible mechanism of imiquimod-induced hypopigmentary lesions. Therefore, we investigated whether imiquimod induces apoptosis of human melanocytes and also whether it modulates expression of apoptosis-related molecules in human melanocytes. Imiquimod treatment induced apoptosis of melanocytes, which was observed by TUNEL assay and Hoechst 33258 staining. Imiquimod-induced apoptosis was further shown by measuring mitochondrial membrane potential in melanocytes. The apoptotic activity of imiquimod was associated with caspa... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3121279 Wed, 23 Dec 2009 22:20:56 +0100 3121279 http://www.medworm.com/index.php?rid=3086146&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fuk70817469116007%2F Abstract  During the morphogenesis of hair follicles, the invasive migration of basal keratinocytes resembles cell’s dissemination of tissue remodeling. The urokinase-type plasminogen activator receptor (uPAR) appears to be a key molecule in the metastasis. In order to elucidate the relationship between uPAR and the invasion of the human hair follicle, immunohistochemistry, RT-PCR, plasmids transfection, and western blot were used. The results showed that uPAR was expressed in the outermost epithelial cells of the hair follicle and the basal keratinocytes of epidermis, and that the expression decreased with the development of the hair follicle. The cells of the outer root sheath (ORS) and interfollicle epidermis, which overexpressed uPAR, acquired increased invasiveness; ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3086146 Sun, 13 Dec 2009 06:41:39 +0100 3086146 http://www.medworm.com/index.php?rid=3086147&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F077605n415558327%2F Abstract  Pathergy is the term used to describe hyper-reactivity of the skin that occurs in response to minimal trauma. A positive skin pathergy test (SPT), characterised by erythematous induration at the site of the needle stick with a small pustule containing sterile pus at its centre, is among the criteria required for a diagnosis of Behçet’s disease (BD) and in certain population has been shown to be highly specific for this condition. Problems with standardising the induction manoeuvre for the SPT as well as the method of assessment of the response have limited the usefulness of the SPT in the clinical setting. Extensive investigation into histopathological and immunological aspects of pathergy has led to a number of hypotheses relating to the aetiology of the skin ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3086147 Sat, 12 Dec 2009 14:16:33 +0100 3086147 http://www.medworm.com/index.php?rid=3086148&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fbxtu533210g4228l%2F Abstract  Laugier–Hunziker syndrome (LHS), a rare, acquired pigmentary disorder of the lips, oral mucosa, and fingers, is known to be an entirely benign disease with no systemic manifestations. In the past, the pigmentation has been treated efficiently in a few patients with the Q-switched neodymium: yttrium–aluminum–garnet (Nd:YAG) laser and the Q-switched alexandrite laser (QSAL). In order to evaluate the efficacy and safety of QSAL on Chinese patients of LHS, we treated 22 patients with QSAL in the past 5 years. Treatments were delivered on a bimonthly or trimonthly basis until the abnormal pigmentation totally disappeared. Patients were evaluated at each visit for evidence of dyspigmentation, scarring, or other untoward effects from the laser treatment. Our 2... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3086148 Thu, 10 Dec 2009 15:16:41 +0100 3086148 http://www.medworm.com/index.php?rid=3064388&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn1h61074221t3638%2F Abstract  Wound infections with multi-drug resistant bacteria increase morbidity and mortality and have considerable socioeconomic impact. They can lead to impaired wound healing, resulting in rising treatment costs. The aim of this study was to investigate an ex vivo human wound infection model. Human full-thickness skin from the operating room (OR) was placed into the Bo-Drum® and cultivated for 7 days in an air–liquid interphase. On day 8, the skin was inoculated with either (1) Pseudomonas aeruginosa, (2) Staphylococcus aureus (105 CFU, n = 3) or (3) carrier control. 1, 3 and 7 days after inoculation colony forming units in the tissue/media were determined and cytokine expression was quantified. A reliable and reproducible wound infection could be es... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3064388 Wed, 02 Dec 2009 18:43:20 +0100 3064388 http://www.medworm.com/index.php?rid=3031820&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F41ht843m6035r278%2F Abstract  The itch–scratch cycle aggravates chronic inflammatory skin diseases. We have previously reported that mice begin to scratch themselves within several minutes after skin-scratching stimulation. This is associated with an increase in release of substance P (SP) from sensory nerve fibers in the skin, and the self-scratching behavior is suppressed by neurokinin-1 receptor (NK-1R) antagonist. Thus, SP may play a pivotal role in self-scratching behavior. The purpose of this study was to investigate the effect of second-generation histamine H1-receptor antagonists on self-scratching behavior in mice. After oral administration of epinastine hydrochloride (epinastine) (total dose 50 ± 5 mg/kg for 7 days) or the vehicle only to ICR mice for 7 day... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3031820 Mon, 23 Nov 2009 16:53:19 +0100 3031820 http://www.medworm.com/index.php?rid=3031819&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc26221vg25x66224%2F Abstract  KIT is an essential receptor that modulates melanocyte function and whose function is disrupted in several pigmentary disorders. However, little is known about the effects of a single UVB exposure on the expression of KIT and two important regulatory transcription factors, MITF and AP-2α, in human melanocytes. We found that a single UVB exposure of human melanocytes induces an early decrease and a subsequent increase in functional KIT expression in concert with up-regulated MITF expression. The increased MITF expression was accompanied by a markedly stimulated and prolonged phosphorylation of p38/CREB. The UVB-stimulated expression of KIT could be completely abolished by a p38 inhibitor, concomitant with a reduced phosphorylation of CREB and a down-regulation of ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3031819 Mon, 23 Nov 2009 16:53:19 +0100 3031819 http://www.medworm.com/index.php?rid=3020214&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F37248g4678771761%2F Abstract  The clinical as well as histological data of 79 mid-dermal elastolysis (MDE) patients reported in the literature were evaluated. MDE is an acquired skin condition of the elastic tissue predominantly manifesting on the trunk and proximal extremities of young women. Most commonly observed skin changes include patches of well-circumscribed fine wrinkles (type I) and perifollicular papular protrusions (type II). Rarely, MDE may also occur with persistent reticular erythema and wrinkling (type III). The critical diagnostic histopathological feature of MDE is the selective loss of elastic fibres in the mid-dermis. Mild lymphohistiocytic infiltrates, elastophagocytosis of elastic fibres by macrophages, and even multinucleate giant cells are occasionally observed in MDE l... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3020214 Sat, 21 Nov 2009 09:08:36 +0100 3020214 http://www.medworm.com/index.php?rid=2990490&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr5t1516882451228%2F Abstract  Many reports provided strong evidence of the influence of genetic factors in the pathogenesis of psoriasis (Ps). A higher prevalence of lipid disorders in psoriatic patients has been reported. Because apolipoprotein E (apoE) is involved in lipid metabolism, APOE gene variants could be candidates to influence Ps-risk. However, data about the potential influence of the APOE genotypes in Ps are inconclusive. Our objective was to investigate the relationship between the common APOE-ε2/ε3/ε4 variation and Ps in a Caucasian population. Our study involved 331 unrelated Ps-patients and 400 healthy controls. Patients and controls were genotyped for the APOE-ε2/ε3/ε4 polymorphism, and allele and genotype frequencies were statistically compared between the two groups and ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2990490 Thu, 12 Nov 2009 18:38:38 +0100 2990490 http://www.medworm.com/index.php?rid=2990491&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh70r616031420085%2F In this report, we identified a novel mutation of CYLD gene in a Chinese family with MFT. It is a novel heterozygous nucleotide G→A transition at position 2,317 in exon 17 of the CYLD gene. Our study expands the database on the CYLD gene mutations in MFT. Content Type Journal ArticleCategory Short CommunicationDOI 10.1007/s00403-009-1003-1Authors Fu-Xi Wang, Shenzhen Second People’s Hospital Department of Dermatology 3002 Sungang West Road 518039 Shenzhen Guangdong ChinaLi-Jia Yang, Nanjing Medical University Department of Dermatology, Affiliated Wuxi No.2 Hospital 214002 Wuxi Jiangsu ChinaMing Li, Nanjing Medical University Department of Dermatology, Affiliated Wuxi No.2 Hospital 214002 Wuxi Jiangsu ChinaShu-Lin Zhang, Shenzhen Second People’s Hospital Department of Dermatolog... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2990491 Thu, 12 Nov 2009 18:38:34 +0100 2990491 http://www.medworm.com/index.php?rid=2982585&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhn15t18121482h33%2F Abstract  Myotonic muscular dystrophy (MMD) is caused by an abnormal function of RNA-binding proteins (RBP) resulting in DNA spliceopathy. A case of a patient, with MMD multiple basal and squamous cell carcinomas and dysplastic nevi, is described. The association between MMD and non-melanoma skin cancer has been reported before; however, this association was described before the genetic defect of myotonic dystrophy has been fully elucidated. The author proposes a genetic mechanism on how abnormal function of RBP can result or contribute to the development of human skin cancer and propose an explanation for this association between MMD and cutaneous carcinogenesis. Content Type Journal ArticleCategory News & ViewsDOI 10.1007/s00403-009-0997-8Authors Alexander Zemtsov, In... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2982585 Tue, 10 Nov 2009 07:55:00 +0100 2982585 http://www.medworm.com/index.php?rid=2971478&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh25k3410146r7rkn%2F Abstract  Epidemiological, clinical and laboratory studies have implicated solar ultraviolet (UV) radiation in various skin diseases including, premature aging of the skin and melanoma and non-melanoma skin cancers. Chronic UV radiation exposure-induced skin diseases or skin disorders are caused by the excessive induction of inflammation, oxidative stress and DNA damage, etc. The use of chemopreventive agents, such as plant polyphenols, to inhibit these events in UV-exposed skin is gaining attention. Chemoprevention refers to the use of agents that can inhibit, reverse or retard the process of these harmful events in the UV-exposed skin. A wide variety of polyphenols or phytochemicals, most of which are dietary supplements, have been reported to possess substantial skin pho... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2971478 Fri, 06 Nov 2009 18:56:30 +0100 2971478 http://www.medworm.com/index.php?rid=2971479&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg026825833q21252%2F Abstract  Epidermal thickness (ET) has been suggested as a surrogate measure of psoriasis severity. Optical coherence tomography (OCT) is a recent imaging technology that provides real-time skin images to a depth of 1.8 mm with a micrometre resolution. OCT may provide an accurate in vivo measure of ET. It is, therefore, speculated that OCT may be used in the assessment of psoriasis vulgaris. A total of 23 patients with psoriasis vulgaris were systematically evaluated by OCT imaging and skin biopsy during treatment. Biopsies were graded for disease severity, and additional evaluation was done by the physician via psoriasis area and severity index (PASI) score, and by the patient through measures such as self-administered PASI, psoriasis life stress inventory index and d... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2971479 Fri, 06 Nov 2009 08:49:01 +0100 2971479 http://www.medworm.com/index.php?rid=2940951&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Feq2x767m03w37x66%2F Abstract  Solar radiation is a very important exogenous factor in skin pathogenesis and can lead to the development of a number of skin disorders. UVB irradiation is known to induce oxidative stress, inflammation and especially DNA lesions in exposed cells. It is important, therefore, to identify agents that can offer protection against UVB-caused skin damage. Natural compounds have been studied for their possible ability to control/modulate various lifestyle-related diseases. The application of plant compounds/extracts with screening, antioxidant and anti-inflammatory activities may also successfully protect the skin against UV-caused injury. We assessed the potency of Prunella vulgaris extract (PVE) and its main phenolic acid component, rosmarinic acid (RA), to suppress UV... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2940951 Wed, 28 Oct 2009 07:10:12 +0100 2940951 http://www.medworm.com/index.php?rid=2924235&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd64m70368m47p7n1%2F In conclusion, ECS exhibits a notable activity in protecting against oxidative stress and interrupting of ROS-ERK1/2-Ha-Ras signal loop in SSc, suggesting its potential protective effects against skin sclerosis. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-009-0998-7Authors Yue-lan Cao, Zhejiang University Department of Dermatology, Second Affiliated Hospital, School of Medicine 88 Jiefang Road 310009 Hangzhou ChinaXin Li, Hangzhou Center for Disease Control and Prevention Department of STD and AIDS Prevention 310006 Hangzhou ChinaMin Zheng, Zhejiang University Department of Dermatology, Second Affiliated Hospital, School of Medicine 88 Jiefang Road 310009 Hangzhou China Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2924235 Thu, 22 Oct 2009 18:56:05 +0100 2924235 http://www.medworm.com/index.php?rid=2917652&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy540167x76g67143%2F Abstract  Systemic sclerosis (SSc) is a connective tissue disease characterized by fibrosis and vascular changes in the skin and internal organs with autoimmune background. It has been suggested that oxidative stress plays an important role in the development of SSc. To determine the prevalence and clinical correlation of autoantibody to methionine sulfoxide reductase A (MSRA), one of the antioxidant repair enzymes, in SSc, serum anti-MSRA autoantibody levels were examined in patients with SSc by enzyme-linked immunosorbent assay using recombinant MSRA. The presence of anti-MSRA antibody was evaluated by immunoblotting. To determine the functional relevance of anti-MSRA antibody in vivo, we assessed whether anti-MSRA antibody was able to inhibit MSRA enzymatic activity. Ser... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2917652 Wed, 21 Oct 2009 09:05:34 +0100 2917652 http://www.medworm.com/index.php?rid=2848146&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5233884387nx1571%2F In this study, the early transcriptional response of human epidermal keratinocytes to EFs is examined using microarrays. Increased expression of various chemokines, interleukins, and other inflammatory response genes indicates that EFs stimulate keratinocyte activation and immune stimulatory activity. Gene expression activity further suggests that interleukin 1 is either released or activated in EFs. Expression of the chemokine CCL20 steadily increases at 100 mV/mm over time until around 8 h after exposure. This chemokine is also expressed at field strengths of 300 mV/mm—above the level of endogenous wound fields. The early effects of EFs on epithelial gene expression activity identified in these studies suggest the importance of naturally occurring EFs both in repa... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2848146 Mon, 28 Sep 2009 14:40:30 +0100 2848146 http://www.medworm.com/index.php?rid=2810771&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F03365701658w1280%2F Abstract  The purpose of this study was to evaluate in vivo the effects of micropuncture injections of stabilized hyaluronic acid-based gel of non-animal origin (NASHA™, Restylane Vital™) on skin elasticity, a major aspect of skin ageing. Patients (n = 19) underwent a series of three treatment sessions, spaced 4 weeks apart, with NASHA injected into the lower facial cheeks. Using the suction principle, 12 parameters describing the viscoelastic properties of the skin were assessed, before each treatment session and at follow-up visits 4 and 16 weeks after the last treatment. Treatment with NASHA significantly increased skin firmness and improved its viscoelastic recovery capacities. The most significant differences from baseline were noted at the end o... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2810771 Thu, 17 Sep 2009 12:07:25 +0100 2810771 http://www.medworm.com/index.php?rid=2810772&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7112782626433051%2F Abstract  Skin keratinocytes constitute a protective mechanical barrier against invading microorganisms. Stimulated keratinocytes produce endogenous peptides such as the β-defensins that have direct antimicrobial activity against a broad spectrum of pathogens, including most bacteria, certain fungi and enveloped viruses. In particular, human β-defensin 2 (HBD-2) is virtually absent in normal skin and its expression in human keratinocytes requires stimulation by cytokines or bacteria. AV119, a patented avocado sugar, triggers the up-regulation of HBD-2, but the signalling mechanisms involved in this up-regulation in stimulated keratinocytes are not fully understood. In the present study, we examined the intracellular signalling pathways and nuclear responses in skin kerati... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2810772 Thu, 17 Sep 2009 12:07:23 +0100 2810772 http://www.medworm.com/index.php?rid=2810774&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx528w62026747k67%2F Abstract  Thioredoxin-1 is a ubiquitous protein involved in phenotypical and functional changes in dendritic cells (DC). We investigated the effect of lipopolysaccharide (LPS), skin sensitizers, and irritants on thioredoxin-1 by Western blot and immunofluorescence and on mRNA by real-time PCR. As DC models, we used a skin DC line and DC derived from human blood monocytes. We observed that all tested chemicals increased thioredoxin-1 expression, which is only transient for irritants, being the strongest effect observed for LPS (63 ± 15%). To address the involvement of thioredoxin-1 in DC maturation, we analysed the effect of an activator of thioredoxin-1 expression, hydrogen peroxide, on CD86 expression, a marker of DC maturation. We found that hydrogen peroxide i... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2810774 Wed, 16 Sep 2009 18:49:13 +0100 2810774 http://www.medworm.com/index.php?rid=2810773&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx4057k3528763131%2F Abstract  The sun emits different types of ultraviolet (UV) light. Our skin is a natural target of UV radiation which is involved in vitamin D3 production in our body. UV radiation at high doses is an environmental carcinogen which can elicit skin damage as well as inducing skin cancer. It can mediate inflammatory and immunological reactions through activation of receptors, DNA/RNA damage and production of reactive oxygen species. It is also involved in the release of pro-inflammatory cytokines, of which TNFα has been implicated in tumorigenic activities. In order to mediate its effects, UV radiation is known to activate multiple signalling cascades such as the p38 MAPK, Jun N-terminal kinase, extracellular signal-regulated kinase 1/2 and NFκB pathways in skin cells. The ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2810773 Wed, 16 Sep 2009 18:49:13 +0100 2810773 http://www.medworm.com/index.php?rid=2735662&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2248g26113448084%2F Abstract  Peripheral blood mononuclear cells (PBMC) collected from subjects prior to treatment and post-treatment with a vaccine composed of leishmania antigens were analyzed by flow cytometry. Upon analysis, it was noticed that lymphocyte subsets (LS) varied with psoriasis area and severity index (PASI) range (1–10, 11–20 and 21–72). Pre-treatment absolute values of gated LS were as follows. CD4+CD8−, CD3+CD8−, CD8+CD3+, CD8+CD4− and CD8+HLA− decreased in PBMC as PASI increased, suggesting migration from the blood to the skin. Contrary to the previous finding, the following LS, CD8+HLA+ and HLA+CD8−, and membrane surface immunoglobulin IgA+, IgD+ and IgM+ increased in PBMC as PASI increased, suggesting activation and proliferation by unknown antigens. After... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2735662 Tue, 25 Aug 2009 16:45:49 +0100 2735662 http://www.medworm.com/index.php?rid=2735664&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq1316j475314jk23%2F Abstract  A recent genome-wide association analysis of psoriasis identified IL12B and IL23R as significantly associated with psoriasis. Here we report association test results of a Thai cohort consisting of 206 psoriasis cases and 114 controls. The IL23R SNPs rs7530511 and rs11209026, and IL12B SNPs rs3212227 and rs6887695 were genotyped using Taqman assays. Data were analyzed using a logistic regression model for linear trend of association. One of the IL23R markers, rs7530511, was marginally significant (P = 0.017). The other IL23R marker, rs11209026, was not polymorphic. One of the IL12B markers, rs3212227, showed significant association with psoriasis (OR = 1.64, P = 0.0058) while the other, rs6887695, did not (OR = 1.29, P =&nbs... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2735664 Tue, 25 Aug 2009 06:54:14 +0100 2735664 http://www.medworm.com/index.php?rid=2735663&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq2633167u3542046%2F Abstract  Keratinocytes are cells strongly exposed to oxidative stress, but normally good equipped for antioxidant responses. However, it has long been suggested that exogenous antioxidants could play a useful role in minimizing the adverse skin responses associated with such oxidant species. In this work it was paid attention to the extract of Rhodiola rosea L. roots by using the phytocomplex as a whole because of the important activity of its composition and mutual distribution of its components. We have measured the protection afforded by the extract to reduced glutathione levels, glyceraldehyde-3-phosphate dehydrogenase activity, and thiobarbituric acid reactive substances levels in cultured human keratinocytes (NCTC 2544) exposed to different oxidative insults: Fe(II)/a... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2735663 Tue, 25 Aug 2009 06:54:14 +0100 2735663 http://www.medworm.com/index.php?rid=2731755&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn0hg305377102306%2F Abstract  β-Catenin is involved in the hair follicle morphogenesis and stem cell differentiation, and inhibition of glycogen synthase kinase-3 (GSK-3) increases β-catenin concentration in the cytoplasm. To examine the effects of GSK-3 inhibition on the hair follicle epithelium, we first examined the expression of GSK-3 in plucked human hair follicles by RT-PCR and found GSK-3 expression in hair follicles. Western blotting with a GSK-3β-specific antibody, Y174, also demonstrated GSK-3β expression in the follicles. Moreover, GSK-3β immunostaining with Y174 showed that GSK-3β colocalized with hair follicle bulge markers. Contrary to GSK-3β, GSK-3α was widely expressed throughout the follicles when immunostained with a specific antibody, EP793Y. We then investigated the... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2731755 Mon, 24 Aug 2009 16:51:51 +0100 2731755 http://www.medworm.com/index.php?rid=2728132&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Flv415257322x8247%2F This study suggests that, especially, dermal components are important in fetal scarless healing. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-009-0989-8Authors Neeltje A. Coolen, Association of Dutch Burn Centres P.O. Box 1015 1940 EA Beverwijk The NetherlandsKelly C. W. M. Schouten, Association of Dutch Burn Centres P.O. Box 1015 1940 EA Beverwijk The NetherlandsEsther Middelkoop, Association of Dutch Burn Centres P.O. Box 1015 1940 EA Beverwijk The NetherlandsMagda M. W. Ulrich, Association of Dutch Burn Centres P.O. Box 1015 1940 EA Beverwijk The Netherlands Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2728132 Sat, 22 Aug 2009 18:53:05 +0100 2728132 http://www.medworm.com/index.php?rid=2723668&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F26201235138k1145%2F Abstract  Behçet’s disease (BD) is a chronic, relapsing, systemic vasculitis of unknown etiology with the clinical features of mucocutaneous lesions, ocular, vascular, articular, gastrointestinal, urogenital, pulmonary, and neurologic involvement. Mucocutaneous lesions figure prominently in the presentation and diagnosis, and may be considered the hallmarks of BD. Therefore, their recognition may permit earlier diagnosis and treatment. Although, the treatment has become much more effective in recent years, BD is still associated with severe morbidity and considerable mortality. The main aim of the treatment should be the prevention of irreversible organ damage. Therefore, close monitoring, early and appropriate treatment is mandatory to reduce morbidity and mortality. We... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2723668 Fri, 21 Aug 2009 15:27:32 +0100 2723668 http://www.medworm.com/index.php?rid=2679839&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fgw5152h1l7251657%2F Abstract  In many skin diseases such as Demodex folliculitis, rosacea- or steroid-induced rosacea Demodex mites are present in abundance and are at least partially held responsible for causing these disorders. Although it is known that these diseases respond well to tetracyclines, it is unclear if this is due to the antiinflammatory effects of the antibiotics or to an antibacterial effect on so far unknown bacteria within the Demodex mites. As in filariasis, where the response to doxycycline can be explained by the presence of Wolbachia within the filarial nematodes, this study was performed to see whether Wolbachia also use Demodex mites as their hosts. Human and canine Demodex mite samples were taken by skin scrapings and tested by PCR for the presence of Wolbachia DNA. Wolba... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2679839 Mon, 03 Aug 2009 20:43:28 +0100 2679839 http://www.medworm.com/index.php?rid=2651435&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa29441wx35n147h1%2F Abstract  As acute burn patients have experienced increasing survival rates, the number of patients who need specific care due to aberrant scarring is also increasing. The burned skin often responds with fibrotic tissue proliferation, which can lead to a hypertrophic scar or a keloid. Non-physiologic scars are mostly not acceptable for the burn patient. Intradermal and topical therapy in burns comprise the treatment of the skin injury and its possible texture, elasticity and color alterations with the aid of active substances that result in fibroblastic modulation. An alteration of cytokine levels may mediate these effects, and evidences suggest that keloid scar formation may be mediated, in part, by deranged growth factor activity, including that of transforming growth fac... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2651435 Mon, 27 Jul 2009 21:49:15 +0100 2651435 http://www.medworm.com/index.php?rid=2638788&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F0415j42h781t7163%2F Abstract  Psoriatic arthritis (PsA) is an inflammatory arthritis associated with irreversible joint damage in a subset of individuals. There is a need to screen early for this condition to prevent damage. To meet this need, we have developed the psoriatic arthritis screening and evaluation (PASE) questionnaire. The 15-item PASE questionnaire was administered to 190 individuals with either psoriasis or PsA. The PASE questionnaire was readministered to a subset of individuals with PsA in order to assess test–retest reliability and sensitivity-to-change. Receiver operator curves were constructed to optimize sensitivity and specificity for the diagnosis of PsA. Of the 190 participating in the study, 19.5% (37/191) participants were diagnosed with PsA. PASE total scores ranged... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2638788 Thu, 23 Jul 2009 08:59:55 +0100 2638788 http://www.medworm.com/index.php?rid=2617913&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl6722u160p671371%2F Abstract  Tuberculosis has emerged as a major concern in patients with immuno-mediated diseases, including psoriasis, undergoing treatment with biologicals. However, it is not known whether the chronically activated immune system of psoriasis patients interferes with their Mycobacterium tuberculosis (Mtb)-specific immunity, especially in tuberculosis-endemic areas like Brazil. We evaluated T-cell responses to a Mtb lysate and to the recombinant Mtb proteins ESAT-6 and Ag85B of tuberculin skin test (TST) positive and TST negative patients with severe or mild/moderate, untreated psoriasis in three different assays: lymphocyte proliferation, enzyme immunoassay for interferon (IFN)-γ and interleukin (IL)-10 production by peripheral blood mononuclear cells and overnight enzyme ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2617913 Sat, 18 Jul 2009 08:26:03 +0100 2617913 http://www.medworm.com/index.php?rid=2617914&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc7807485j871wg17%2F Abstract  Evidence-based psoriasis treatment guidelines may help physicians overcome uncertainties in initiating and monitoring systemic treatment and have thus been suggested as a tool for improving the care provided to psoriasis patients. A prospective cohort study of dermatologists’ prescription behaviour was performed with two consecutive documentation periods (before and after the publication of the guidelines). 49 dermatologists were asked to provide continuous documentation of their treatment choices for patients with psoriasis by filling in a standardised documentation form. In addition, a questionnaire consisting of eight items was used to assess familiarity with, attitudes towards, and the impact of the psoriasis guidelines on the participating dermatologists. 4... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2617914 Sat, 18 Jul 2009 08:26:00 +0100 2617914 http://www.medworm.com/index.php?rid=2603770&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F52355m8737g65435%2F Abstract  In skin inflammation, vascular endothelial growth factor (VEGF) and CXCL-8/IL-8 play an important role and are produced by activated keratinocytes. Extracts from Ginkgo biloba leaves (GBE), widely used in phytotherapy, have been reported to exert antioxidant and anti-inflammatory properties in the skin. We therefore evaluated the effects of GBE on the release of VEGF and CXCL8/IL-8 by normal human keratinocytes (NHKs) activated by tumor necrosis factor α (TNFα). Moreover, as we previously showed that epigallocatechin-3-gallate (EGCG) reduces VEGF and CXCL8/IL-8 secretion in TNFα-activated NHKs, we also tested its effect in association with GBE. Our results showed that GBE exerted a potent inhibition on VEGF and CXCL8/IL-8 levels in activated cells. In associati... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2603770 Tue, 14 Jul 2009 09:56:44 +0100 2603770 http://www.medworm.com/index.php?rid=2589542&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu3l01486414535t5%2F This study could not verify significant damage to skin barrier after 7 days of repeated occlusion 4 h daily in healthy volunteers. These findings require further discussion regarding the definitions in the current German ordinance on hazardous substances and in the TRGS 401. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-009-0980-4Authors Ulrike Wetzky, University of Osnabrueck Department of Dermatology, Environmental Medicine and Health Theory Sedanstrasse 115 49090 Osnabrueck GermanyMeike Bock, University of Osnabrueck Department of Dermatology, Environmental Medicine and Health Theory Sedanstrasse 115 49090 Osnabrueck GermanyBritta Wulfhorst, University of Osnabrueck Department of Dermatology, Environmental Medicine and Health Theory Sedanstrasse ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2589542 Tue, 07 Jul 2009 15:24:28 +0100 2589542 http://www.medworm.com/index.php?rid=2589543&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fug0135l518316858%2F In this study, we investigated the effects of erythromycin A (EMA) and its new derivative EM201 on type I collagen production by cultured dermal fibroblasts. Dermal fibroblasts were cultured with 10−9 M-10−5 M EMA or EM201, and collagen production was measured by incubation with radioactive proline, SDS-polyacrylamide gel electrophoresis and fluorography. mRNA levels were measured by Northern blots analysis, and to investigate transcriptional levels luciferase assays were also performed. The results showed that both EMA and EM201 increased collagen production and type I collagen mRNA level (to a maximum of 200% with EMA and 250% with EM201) in a dose-dependent manner in cultured dermal fibroblasts. Transcription of the type I collagen gene was also increased by both macrolides. Th... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2589543 Mon, 06 Jul 2009 20:15:08 +0100 2589543 http://www.medworm.com/index.php?rid=2589544&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft7q5gu4v839500t7%2F Abstract  Impaired local cellular immunity is one of the mechanisms responsible for condyloma acuminatum (CA) recurrence. The activation of dendritic cells (DCs) is important in vaccine development. We investigated the effect of different toll like receptor (TLR) agonists including LPS (TLR4 agonist), polyinosinic acid-polycytidylic acid (PIC, TLR3 agonist), CpG oligonucleotide (TLR9 agonist), and imiquimod (TLR7 agonist) on human monocyte-derived dendritic cells (mdDCs) loading of human papillomavirus (HPV) type 11 E7 epitope. As a result, we found that mdDCs loading HLA-A*0201-restricted HPV 11 E7 CTL epitope peptide could respond to the TLR agonists, especially LPS and PIC. This was characterized by an enhanced expression of CD40, CD80, CD86, CD83 and HLA-DR, and a high ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2589544 Mon, 06 Jul 2009 20:15:07 +0100 2589544 http://www.medworm.com/index.php?rid=2515934&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr4l0u5537620052j%2F Abstract  Mutations in three functionally related genes EDA, EDAR and EDARDD have been reported to cause hypohidrotic ectodermal dysplasia (HED), which is characterized by sparse hair, reduced ability to sweat, and hypodontia. In few cases mutations in the EDA gene have been found to result in X-linked recessive isolated hypodontia. In the study, presented here, we have ascertained two large Pakistani families (A and B) with autosomal recessive form of hypohidrotic ectodermal dysplasia and X-linked recessive isolated hypodontia. Genetic mapping showed linkage of family A to EDAR gene on chromosome 2q11-q13 and family B to EDA gene on chromosome Xq12-q13.1. Subsequently, DNA sequencing of the coding regions of EDAR and EDA genes revealed previously described mutations. Sequence... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2515934 Wed, 24 Jun 2009 06:05:38 +0100 2515934 http://www.medworm.com/index.php?rid=2488455&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F62857v4411747p86%2F Abstract  New topical immunomodulators have been reported to cause repigmentation of vitiligo lesions. However, time-kinetics of such repigmentation in different anatomic locations is not well known. We performed a randomized double-blind placebo control study with tacrolimus versus the vehicle and a nonrandomized control study with pimecrolimus to evaluate the time to reach significant pigmentation, its duration and extent in treated areas. Antioxidant status of serum was also assessed. Twenty patients, in the tacrolimus study, had one pair of lesions on different localizations, and 20 on face and/or upper limbs for pimecrolimus. The extent of repigmentation was evaluated by slides and mapmakings at baseline and every 4 weeks during 7 months. Adverse events were ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2488455 Tue, 23 Jun 2009 06:02:41 +0100 2488455 http://www.medworm.com/index.php?rid=2488456&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd03326389p75x1m7%2F Abstract  Examining the patients with a new variant of endemic pemphigus foliaceus (EPF) in El Bagre, Colombia, (El Bagre-EPF), we noted several polymorphic clinical lesions around their axillary areas. Based on our clinical findings and on previous histopathological studies on the skin of these patients that showed abnormalities in their sweat glands, and the presence of mercuric selenides and iodines by autometallography assay, we decided to investigate immunoreactivity to the sweat glands in these patients. We tested for autoreactivity utilizing direct and indirect immunofluorescence (DIF, IIF). To be able to distinguish between non-specific immune deposits and real autoimmune response, and knowing that sweat glands have some intrinsic autofluorescence for the presence o... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2488456 Tue, 23 Jun 2009 06:02:40 +0100 2488456 http://www.medworm.com/index.php?rid=2488457&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F573298563k42t704%2F Abstract  The goal of this study is to investigate commercially available cosmetics (foundations, skin care creams) which also claim to include a sun protection factor (SPF). Are these products, which are not considered sunscreen products, helpful or could they be harmful? Using an in vitro method, we tested the effectiveness of 35 commercially available products against UVB and UVA radiation. For each product, our testing focused on determining the following four values in terms of current legal recommendations: SPF, UVA protection factor (PF-UVA), UVB/UVA ratio and critical wavelength (λc). We also tested each product’s level of photostability. Effectively, when considering instructions for use (skincare products are applied once, in the morning) any product displaying ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2488457 Sat, 20 Jun 2009 06:01:16 +0100 2488457 http://www.medworm.com/index.php?rid=2488459&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc4375602w38073w4%2F In conclusion, lacking TNFα potentiates Smad-mediated fibrogenic reaction in healing dermis and retards reepithelialization in a healing mouse cutaneous wound. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-009-0969-zAuthors Maki Shinozaki, Wakayama Medical University School of Medicine Department of Critical Care Medicine 811-1 Kimiidera Wakayama 641-0012 JapanYuka Okada, Wakayama Medical University School of Medicine Department of Ophthalmology 811-1 Kimiidera Wakayama 641-0012 JapanAi Kitano, Wakayama Medical University School of Medicine Department of Ophthalmology 811-1 Kimiidera Wakayama 641-0012 JapanKazuo Ikeda, Nagoya City University Department of Functional Anatomy 1, Sumikawa, Mizuho Nagoya 467-8601 JapanShizuya Saika, Wakayama Medical University Sc... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2488459 Sat, 20 Jun 2009 06:01:15 +0100 2488459 http://www.medworm.com/index.php?rid=2488458&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv77529008k2w6n63%2F We report a novel point mutation at donor splice-site in intron 42 of type III collagen gene resulting in the inclusion of 30 nucleotides into the mature mRNA in a case of vascular type of EDS. Since the age of approximately 8 months, the patient had had repeated episodes of purpura and gradually developed thin, translucent skin. She had a past history of pneumothorax. At the initial examination, she was found to have the characteristic facies, i.e., bird-like face, of the vascular type of EDS, thinning of skin over the limbs and trunk, and scattered purpura. The blood vessels under the skin could be clearly visualized. She showed hypermobility of the small joints of all the four limbs and acrogeric changes of the hands and feet. Analysis of the amount of collagen synthesized fr... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2488458 Sat, 20 Jun 2009 06:01:15 +0100 2488458 http://www.medworm.com/index.php?rid=2488460&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd86406r5568w8u20%2F Abstract  Hypotrichosis simplex comprises a group of non-syndromic human alopecias. Diffuse loss of hair typically starts in early childhood and progresses throughout adolescence. We and others have previously reported mutations in the P2RY5 gene and the LIPH gene as being causal factors of autosomal recessive hypotrichosis simplex with or without woolly hair. In the present study, we analyzed one Turkish family and two non-related girls of Indian ethnicity affected with hypotrichosis and woolly hair for mutations in these genes. We identified as yet unreported mutations in the P2RY5 gene: a 1-base pair deletion (c.472delC) and a 4-base pair duplication (c.64_67dupTGCA), both of which lead to frameshifts resulting in truncated proteins. Our study increases the spectrum of kno... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2488460 Tue, 16 Jun 2009 05:50:07 +0100 2488460 http://www.medworm.com/index.php?rid=2476342&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw9626246121x3234%2F This study characterized, for the first time, the deterioration of right-handed ds-B-DNA and the increase in denatured ss-DNA during epidermal maturation. For the first time, this approach also allowed for the quantitative and qualitative characterization of DNA content and structure in all epidermal strata, using anti-ds-B-DNA and anti-ss-DNA antibodies. In order to improve the retention and quality of DNA, a novel histotechnological processing procedure was used. The results indicate that the largest decline in DNA occurred within the stratum granulosum, followed by the EPTZ, and the stratum spinosum. Not all epidermal nuclei lost DNA, indicating two differentiating keratinocyte pathways, viz., apoptotic and non-apoptotic. Both pathways united in the stratum granulosum. These resul... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2476342 Thu, 11 Jun 2009 13:07:48 +0100 2476342 http://www.medworm.com/index.php?rid=2476341&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F651552t7700070j0%2F Abstract  In psoriasis, leukocytes that infiltrate skin lesions have been shown to be involved in the pathogenesis of this disease. Previous investigations reporting the presence of CXCR3+ T lymphocytes in psoriatic lesional skin have suggested a role of this receptor in the recruitment of T cells into the lesion. The purpose of this study was to quantify the mRNA levels of CXCR3 and to perform a systematic analysis of the cell populations that express CXCR3 in human lesional and non-lesional psoriatic biopsies. We showed by real-time reverse transcriptase-polymerase chain reaction that the mRNA levels of CXCR3 and its ligands, CXCL9-11, were significantly elevated in psoriatic lesions, as compared to non-lesional samples. Serial cryostat sections of psoriasis skin biopsies ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2476341 Thu, 11 Jun 2009 13:07:48 +0100 2476341 http://www.medworm.com/index.php?rid=2459621&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe3503j0655p4447m%2F Abstract  Polymorphism in the apolipoprotein E gene has been associated with several neurological, cardiological and ophthalmological diseases. Apolipoprotein E is involved in psoriasis by modifying mitogen-activated T lymphocyte proliferation and by assuring protection against some infections. We evaluated the apolipoprotein E gene polymorphism in patients suffering from psoriasis (compared to matched controls) in Thrace, Northern Greece. One hundred and forty patients suffering from psoriasis vulgaris and 155 matched controls were included in this study and genotyped by semi-nested polymerase chain reaction RFLPs; the results were evaluated by conditional logistic regression. In psoriasis vulgaris patients, the e2 allele showed higher frequency (6.1%, P = 0.021) ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2459621 Fri, 05 Jun 2009 09:47:16 +0100 2459621 http://www.medworm.com/index.php?rid=2459622&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd08342346w84r7v3%2F Abstract  The case of a 44-year-old man with a primary cutaneous large B-cell non-Hodgkin’s lymphoma of the scalp is reported. His mother died of gastric lymphoma and his sib brother is in a 20-year remission of T-cell lymphoma. The patient presented with a 16-year history of occipital and parietal alopecia and a recently worsening scalp rash. The histopathology and immunohistochemistry performed in April 2006 indicated a bcl-6+, MUM− and bcl-2−, primary cutaneous follicle center B-cell non-Hodgkin’s lymphoma, with an aggressive transformation to a diffuse large B-cell lymphoma. Bone marrow biopsy and CT chest, abdomen, and pelvis were negative for systemic lymphoma. The patient had an excellent clinical and histological resolution following 8 cycles of rituximab an... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2459622 Thu, 04 Jun 2009 10:05:34 +0100 2459622 http://www.medworm.com/index.php?rid=2459623&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq58602w412656x24%2F Abstract  Darier’s disease (DD, MIM 124200) also known as Darier-White disease and keratosis follicularis, is a rare autosomal dominant skin disorder characterized by warty papules and plaques in the seborrheic area (central trunk, flexures, scalp, and forehead). Pathogenic mutations in the ATP2A2 gene encoding the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA) 2 gene underlie the disease. In the present study, we performed genetic investigation of three unrelated Tunisian families affected by DD. Mutation screening was performed by direct sequencing of the coding region and exon/intron boundaries of the ATP2A2 gene. Patients in the 3 studied families exhibited classical DD phenotype. DD was associated with neurological and cardiac disorders in one family. Two nove... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2459623 Tue, 02 Jun 2009 07:52:32 +0100 2459623 http://www.medworm.com/index.php?rid=2459624&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8645jg3446414x01%2F Abstract  Vitiligo is an acquired skin disease, characterized by white areas on the skin due to loss of functional melanocytes. The pathogenesis of the disease is still unclear. Published data show the involvement of oxidative stress in the pathophysiology of vitiligo. A total of 30 vitiligo patients and 30 healthy controls were included in this study. We estimated serum levels of malondialdehyde (MDA), vitamins E and C, total antioxidant activity and whole blood levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in vitiligo patients and controls. We found significantly higher levels of MDA and significantly lower levels of SOD, GPx, vitamins C and E and total antioxidant activity in vitiligo patients compared with controls. This study is a maiden attempt... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2459624 Tue, 02 Jun 2009 07:52:31 +0100 2459624 http://www.medworm.com/index.php?rid=2459625&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F0212p8227014178k%2F Abstract  The aim of the study was to investigate the expression of PGP 9.5 in cutaneous keratoacanthomas (KAs) and squamous cell carcinomas (SCCs). Thirty-one cases of KA (10 in the growth stage, 9 in the mature phase and 12 in the involution stage) and 36 SCCs including 13 well differentiated cases, 12 moderately differentiated tumors, 7 poorly differentiated lesions and 4 pseudoadenoid entities were investigated. PGP 9.5 expression was positively correlated with tumor stage (P < 0.001) and potential perineural invasion (P < 0.001). There was no significant difference in the distribution of patients presenting variable levels of PGP 9.5 staining with regard to maximal tumor size and the extent and degree of stromal invasion. PGP 9.5 expression prov... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2459625 Tue, 02 Jun 2009 07:52:30 +0100 2459625 http://www.medworm.com/index.php?rid=2443607&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F16u130867525778u%2F Abstract  New biologic therapies focused primarily on cytokine pathways, some targeting T cell-mediated immune responses, are being developed for the treatment of psoriasis. Siplizumab is a humanized anti-CD2 monoclonal antibody that interferes with costimulation necessary for T cell activation and proliferation. We assessed the biological activity, serum concentrations, and pharmacodynamic effects of siplizumab in patients with plaque psoriasis. Two multicenter, phase II randomized, double-blind, placebo-controlled studies were conducted: one study randomized 124 patients to one of two intravenous (IV) doses (0.012 and 0.04 mg/kg) of siplizumab, given every 2 weeks × 8 doses; the other study randomized 420 patients to one of three subcutaneous (SC) dose... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2443607 Wed, 27 May 2009 06:03:28 +0100 2443607 http://www.medworm.com/index.php?rid=2433730&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff453q47631h4224r%2F Abstract  Peroxisome proliferator-activated receptor-γ (PPAR-γ) ligands have been recently reported to have beneficial effects on organ fibrosis. However, their effects on extracellular matrix (ECM) turnover in hypertrophic scar fibroblasts (HSFs), and the related molecular mechanisms are unknown. HSFs were cultured and exposed to different concentration PPAR-γ ligands in the presence of transforming growth factor-β1 (TGF-β1). In growth-arrested HSFs, a PPAR-γ natural ligand (15-deoxy-D12,14-prostaglandin J2, 15d-PGJ2) and a synthetic ligand (GW7845) dose-dependently attenuated TGFβ1-induced expression of Connective tissue growth factor (CTGF), collagens and fibronectin. Furthermore, the suppression of CTGF mRNA and protein expression are relieved by pretreatment wit... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2433730 Sun, 24 May 2009 06:09:12 +0100 2433730 http://www.medworm.com/index.php?rid=2433731&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7q484078565m6w13%2F Abstract  In a previous study, 7-week treatment of normal human skin with two test moisturizers, Complex cream and Hydrocarbon cream, was shown to affect mRNA expression of certain genes involved in keratinocyte differentiation. Moreover, the treatment altered transepidermal water loss (TEWL) in opposite directions. In the present study, the mRNA expression of genes important for formation of barrier lipids, i.e., cholesterol, free fatty acids and ceramides, was examined. Treatment with Hydrocarbon cream, which increased TEWL, also elevated the gene expression of GBA, SPTLC2, SMPD1, ALOX12B, ALOXE3, and HMGCS1. In addition, the expression of PPARG was decreased. On the other hand, Complex cream, which decreased TEWL, induced only the expression of PPARG, although not confir... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2433731 Sun, 24 May 2009 06:09:11 +0100 2433731 http://www.medworm.com/index.php?rid=2433732&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm76725q2k2629r67%2F In this study, we investigated the changes in S100A8 mRNA and protein following acute UV irradiation to human buttock skin and by intrinsic aging and photoaging in human sun-protected (upper-inner arm) and sun-exposed (forearm) skin of elderly subjects. Real-time PCR, western blot and immunohistochemical staining analyses of UV-irradiated young buttock skin revealed that S100A8 protein expression was increased at 24 h (3.0-fold) and 48 h (4.4-fold) after UV irradiation. S100A8 mRNA and protein were more highly expressed by 2.3- and 4.0-fold, respectively, in the sun-protected skin of elderly people than in that of young people. In addition, the sun-exposed skin of elderly expressed more S100A8 mRNA and protein than the sun-protected skin of the same individuals. In immunohi... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2433732 Sun, 24 May 2009 06:09:10 +0100 2433732 http://www.medworm.com/index.php?rid=2407374&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg441737176652250%2F This study contributes to the database on KIT in piebaldism and enriches the knowledge about the genotype/phenotype correlation. Content Type Journal ArticleCategory Short CommunicationDOI 10.1007/s00403-009-0955-5Authors Xian-Yong Yin, Anhui Medical University Institute of Dermatology 81 Meishan Road 230032 Hefei Anhui People’s Republic of ChinaYun-Qing Ren, Anhui Medical University Institute of Dermatology 81 Meishan Road 230032 Hefei Anhui People’s Republic of ChinaSen Yang, Anhui Medical University Institute of Dermatology 81 Meishan Road 230032 Hefei Anhui People’s Republic of ChinaSheng-Xin Xu, Anhui Medical University Institute of Dermatology 81 Meishan Road 230032 Hefei Anhui People’s Republic of ChinaFu-Sheng Zhou, Anhui Medical University Institute of Dermatology 81... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2407374 Sat, 09 May 2009 05:46:52 +0100 2407374 http://www.medworm.com/index.php?rid=2391478&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fxj2tt67940426p58%2F Abstract  Numerous studies have examined the effect of local and global heating of the body on skin blood flow. However, the effect of the moisture content of the heat source on the skin blood flow response has not been examined. Thirty-three subjects, without diabetes or cardiovascular disease, between the ages of 22 and 32 were examined to determine the relationship between the effects of dry vs. moist heat applied for the same length of time and with the skin clamped at the same skin temperature on the blood flow response of the skin. The skin, heated with an infrared heat lamp (skin temperature monitored with a thermocouple) to 40°C for 15 min, was either kept moist with wet towels or, in a separate experiment, kept dry with Drierite (a desiccant) between the towe... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2391478 Tue, 05 May 2009 06:07:22 +0100 2391478 http://www.medworm.com/index.php?rid=2391479&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fmwk85j5r96803p28%2F Abstract  Heme oxygenase-1 (HO-1) has been shown to exert immunosuppressive, anti-inflammatory, anti-apoptotic and anti-proliferative effects. Its unique positive effects indicate that this enzyme might be a potential therapeutic target for psoriasis. To determine the effect of pharmacologic up-regulation of HO-1 on psoriasis, we generated a guinea pig model of psoriasiform skin lesions using propranolol induction. In this in vivo model, the 3-week propranolol challenge generated the psoriasiform pathological changes on the ears of guinea pigs. And then, guinea pigs were intraperitoneally injected with 5 mg/kg cobalt protoporphyrin (CoPP), a potent HO-1 inducer, 20 mg/kg zinc protoporphyrin, an inhibitor of HO-1, or PBS as controls, once a week from 9 weeks o... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2391479 Tue, 05 May 2009 06:07:20 +0100 2391479 http://www.medworm.com/index.php?rid=2370759&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe762788n55337611%2F Abstract  As individuals age, the skin undergoes changes, such as irregular pigmentation, thinning and loss of elasticity, that are due to both genetic and environmental factors. These changes may worsen, progressing to precancerous and cancerous diseases. Various medical treatments and topical cosmeceuticals have been used to treat some symptoms of photoaging, however, the results have been less than satisfactory. Mesenchymal stem cells within the stromal-vascular fraction of subcutaneous adipose tissue, adipose-derived stem cells (ADSCs), display multi-lineage developmental plasticity and secrete various growth factors that control and manage the damaged neighboring cells. Recently, the production and secretion of growth factors has been reported as an essential function ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2370759 Sun, 26 Apr 2009 05:46:15 +0100 2370759 http://www.medworm.com/index.php?rid=2366047&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn85347034028u151%2F In conclusion, despite the skin represent a reservoir for genotype-1, parvovirus infection is not involved in the etiopathogenesis of CTCL. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-009-0954-6Authors Francesca Sidoti, Turin University Virology Unit, Department of Public Health and Microbiology Turin ItalyMaria Teresa Fierro, Turin University Dermatology Section, Department of Biomedical Science and Human Oncology Via Cherasco 23 10126 Turin ItalyCristina Costa, Turin University Virology Unit, Department of Public Health and Microbiology Turin ItalyRenata Ponti, Turin University Dermatology Section, Department of Biomedical Science and Human Oncology Via Cherasco 23 10126 Turin ItalyMassimiliano Bergallo, Turin University Virology Unit, Department of Public... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2366047 Thu, 23 Apr 2009 08:26:32 +0100 2366047 http://www.medworm.com/index.php?rid=2354683&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F41871vj410953526%2F Abstract  Tattooing is an ancient art and is still widely practiced all over the world. Since the biocompatibility of tattoo dyes has not been well researched, we studied the toxicity of a commercial tattoo ink, commonly used in tattoo lab and esthetic centers, on human fibroblasts. To test cell viability, MTT assays were carried out and scanning electron microscopy to visualize changes in the cell surface after the dye exposure was performed. A possible influence of the pigment on the expression of procollagen α1 type I protein was visualized by western blotting analysis. The results showed a reduction in cell viability, and electron microscopy demonstrated an unmodified cell surface completely covered by pigment particles. Western blotting analysis demonstrated a clear i... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2354683 Fri, 17 Apr 2009 07:28:57 +0100 2354683 http://www.medworm.com/index.php?rid=2354684&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj68lu7552147846j%2F Abstract  The extracellular matrix (ECM) that gives tissue its structural integrity is remodeled in skin aging/photoaging and cancer via the increased expression/activities of matrixmetalloproteinases (MMP), inhibition of the tissue inhibitors of matrix metalloproteinases (TIMP), or inhibition of collagen synthesis. Transforming growth factor-β (TGF-β), a predominant regulator of the ECM, is inhibited in aging/photoaging and stimulated in carcinogenesis. P. leucotomos (fern) extract has potential to counteract these alterations via its antioxidant, anti-inflammatory and photoprotective properties. The goal of this research was to determine the efficacy of P. leucotomos to (a) directly inhibit MMP-1, 2, 3, and 9 activities, (b) inhibit MMP-2, and stimulate TIMPs, fibrillar c... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2354684 Fri, 17 Apr 2009 07:28:55 +0100 2354684 http://www.medworm.com/index.php?rid=2332433&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fxp6tl14156q2l1u2%2F Abstract  The expression of matrix metalloproteinase-2 was observed to be significantly upregulated in psoriasis. The aim of this study was to associate the DNA polymorphic variants in MMP-2 promoter gene with psoriasis and/or with psoriasis phenotypes related to psoriasis and comorbid heredity. In the total of 582 Czech Caucasian individuals (386 patients with psoriasis and 196 controls of similar age and sex distribution without personal or family history of chronic disease of the skin), four MMP-2 promoter polymorphisms (−1575G/A, −1306C/T, −790T/G and −735C/T) were detected by PCR methods. A significant association of GG genotype of −790 MMP-2 polymorphism with psoriasis was observed (Pcorr = 0.04). Although no significant case–control differences i... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2332433 Fri, 10 Apr 2009 05:57:38 +0100 2332433 http://www.medworm.com/index.php?rid=2332434&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm5806tq963m337u3%2F Abstract  Wound healing is a fundamental complex-tissue reaction leading to skin reconstitution and thereby ensuring survival. While, fetal wounds heal without scarring, a normal “fine line” scar is the clinical outcome of an undisturbed wound healing in adults. Alterations in the orchestrated wound healing process result in hypertrophic or keloid scarring. Research in the past decades attempted to identify genetic, cellular, and molecular factors responsible for these alterations. These attempts lead to several new developments in treatments for keloids, such as, imiquimod, inhibition of transforming growth factor beta, and recombinant interleukin-10. The urgent need for better therapeutics is underlined by recent data substantiating an impaired quality of life in kelo... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2332434 Fri, 10 Apr 2009 05:57:37 +0100 2332434 http://www.medworm.com/index.php?rid=2332435&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F43387820r5g7080w%2F Abstract  Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at the wound margin. When human epidermal keratinocytes were incubated on polymerized type I collagen, they rapidly attached and spread. The cells underwent a proliferative response and, over the subsequent 6-day period, covered the collagen surface with a monolayer of cells. When keratinocytes were plated on collagen that had been fragmented by exposure to matrix metalloproteinase-1 (MMP-1, collagenase-1), the cells attached as readily as to intact collagen but spread more slowly and less completely. Growth was reduced by approximately 50%. Instead of covering the collagen surface, the keratinocytes remained localized to the site of attachment. Keratinocytes on fragment... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2332435 Wed, 08 Apr 2009 08:19:49 +0100 2332435 http://www.medworm.com/index.php?rid=2332436&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg2g345580u770214%2F In conclusion, our studies demonstrate that barrier disruption due to acetone treatment is mainly due to removal of corneocytes. Content Type Journal ArticleCategory Short CommunicationDOI 10.1007/s00403-009-0946-6Authors Robert Rissmann, Leiden University Department of Drug Delivery Technology, Leiden/Amsterdam Center for Drug Research (LACDR) P.O. Box 9502 2300 RA Leiden The NetherlandsMarion H. M. Oudshoorn, Utrecht University Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS) Utrecht The NetherlandsWim E. Hennink, Utrecht University Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS) Utrecht The NetherlandsMaria Ponec, Leiden University Department of Drug Delivery Technology, Leiden/Amsterdam Center for Drug Research (L... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2332436 Tue, 07 Apr 2009 05:47:57 +0100 2332436 http://www.medworm.com/index.php?rid=2306420&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe5uq1r1108w68457%2F Abstract  Recent studies have demonstrated the efficacy of PUVA (psoralen plus ultraviolet A irradiation) therapy against sclerotic skin lesions in scleroderma, although the mechanisms underlying the improvement of the skin sclerosis by this therapy remain unknown. We investigated the effects of ultraviolet A (UVA) irradiation following the treatment with 8-methoxypsoralen on types I and III collagen synthesis and the gene expression of collagenase in cultured normal and scleroderma fibroblasts. The treatment reduced types I and III collagen synthesis and consequently, the types I and III collagen mRNA levels, in a UVA dose-dependent manner in both the normal and SSc fibroblasts, whereas the mRNA levels of collagenase remained almost unaltered. These results ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2306420 Fri, 03 Apr 2009 07:02:12 +0100 2306420 http://www.medworm.com/index.php?rid=2306421&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv86p81v6qr24l098%2F Abstract  We have examined coordinated integrin and growth factor regulation of primary keratinocyte migration mediated by phosphoinositide 3-kinase (PI3K) and mitogen-activated extracellular-regulated kinase (MEK)/extracellular signal-regulated kinase (ERK). On collagen I and fibronectin substrates, both epidermal growth factor (EGF) and hepatocyte growth factor (HGF) stimulated chemokinetic (random) and chemotactic (directional) migration. On provisional matrix, a combination of fibronectin and fibrin found in the early phase of wound healing, EGF and HGF-stimulated significant chemotactic but little or no chemokinetic cell movement. Blocking mAbs to integrin α2β1 and α5β1 effectively inhibited EGF- and HGF-stimulated chemokinetic and chemotactic cell movement on coll... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2306421 Sat, 28 Mar 2009 08:55:25 +0100 2306421 http://www.medworm.com/index.php?rid=2306422&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw8101q4x025697t7%2F This study was designed to chart the importance of these escape mechanisms, and to assess the relationship between gene expression and the clinical profile (especially survival data) of patients with melanoma. We studied the expression of certain melanoma genes in tissue biopsies from 202 patients using reverse transcriptase-polymerase chain reaction (RT-PCR). The evaluated genes were Melan-A, tyrosinase, Na-17A, MAGE-1, MAGE-3 and Ny-ESO-1. We then correlated the results to the patients’ survival data. 202 samples (cutaneous, nodal and visceral biopsies) were analysed by RT-PCR. No relationship was found between clinical data and gene expression. No relationship was found between survival data and gene expression, when samples of all stages were combined in the analysis. However, ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2306422 Fri, 27 Mar 2009 06:53:29 +0100 2306422 http://www.medworm.com/index.php?rid=2306423&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd87573018x45385j%2F This study aimed at developing and validating an instrument on patient-defined needs and benefits in dermatology. The questionnaire was developed according to international guidelines. The benefit assessment consists of two steps: before treatment, every patient defines his treatment needs according to a standardized list. After treatment, the patient rates the degree of benefits achieved. A “patient benefit index” (PBI) is calculated by averaging the preference-weighed results of all items. The PBI questionnaire was validated in a sample of 500 patients with ten skin diseases and in a treatment study on 906 patients with acne. The patients defined a broad spectrum of needs and treatment benefits, indicating disease-specific patterns. The PBI showed good feasibility, reliability ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2306423 Fri, 27 Mar 2009 06:53:28 +0100 2306423 http://www.medworm.com/index.php?rid=2306424&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx00306unv0167226%2F This study was conducted to investigate the effects of copper–GHK on keratinocytes. Proliferative effects were analyzed and hematoxylin and eosin staining and immunohistochemistry were conducted to evaluate the effects of copper–GHK in skin equivalent (SE) models. In addition, western blotting was performed. In monolayer cultured keratinocytes, copper–GHK increased the proliferation of keratinocytes. When the SE models were evaluated, basal cells became cuboidal when copper–GHK was added. Immunohistochemical analysis revealed that copper–GHK increased proliferating cell nuclear antigen (PCNA) and p63 positivity. Furthermore, the expression of integrin α6 and β1 increased in SE models, and these results were confirmed by Western blotting. The results of this study indicate... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2306424 Wed, 25 Mar 2009 06:49:33 +0100 2306424 http://www.medworm.com/index.php?rid=2306425&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft66476l287602777%2F Abstract  T cells are present in normal adult human skin, but their occurrence in fetal skin is unknown. T cell and Langerhans cell (LC) populations were studied using single or double immunohistochemical staining on cryostat-section. Skin samples taken from different body regions of 17 fetuses ranging from 18 to 30 weeks estimated gestational-age (w-EGA), were examined. In all specimens but one, we did not find any epidermal T cell. In contrast, dermal CD3+ T cells occurred at all w-EGA. The density of these cells increased with increasing age. Double staining showed that CD3+ T cells were predominantly CD4+/CD45RA+. On the other hand, LC, as assessed by CD1a expression, was evenly distributed within the interfollicular epidermis and papillary dermis at all gestational ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2306425 Tue, 24 Mar 2009 07:08:09 +0100 2306425 http://www.medworm.com/index.php?rid=2287648&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhw42530376110411%2F Abstract  Cell–matrix interactions are of significant importance for tissue homeostasis of the skin and, if disturbed, may lead to ageing and hyperplastic scar formation. We have studied fibroblasts stably overexpressing manganese superoxide dismutase (MnSOD) with a defined capacity for the removal of superoxide anions and concomitant accumulation of hydrogen peroxide to evaluate the role of enhanced MnSOD activity on the dynamics of cell–matrix interactions in the three-dimensional collagen lattice contraction assay. MnSOD overexpressing fibroblast populated collagen lattices revealed a significantly enhanced contraction compared to collagen lattices populated with vector control cells. The enhanced collagen lattice contraction was in part due to an increase in active ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2287648 Sun, 22 Mar 2009 06:11:35 +0100 2287648 http://www.medworm.com/index.php?rid=2287650&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4626008477p67585%2F In conclusion, we showed that IAA/IPL induces melanoma regression in B16F10 melanoma-implanted nude mice. These results suggest the potential use of IAA/IPL in the treatment of malignant melanoma. Content Type Journal ArticleCategory Short CommunicationDOI 10.1007/s00403-009-0938-6Authors So-Young Kim, Chung-Ang University Department of Biochemistry, College of Medicine 221 Heukseok-Dong Dongjak-Gu Seoul 156-756 Republic of KoreaMyo-Kyoung Kim, University of the Pacific Thomas J. Long School of Pharmacy Stockton CA USASun-Bang Kwon, Seoul National University College of Medicine Department of Dermatology 28 Yongon-Dong, Chongno-Gu Seoul 110-744 Republic of KoreaJung-Im Na, Seoul National University College of Medicine Department of Dermatology 28 Yongon-Dong, Chongno-Gu Seoul 110-744 ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2287650 Fri, 20 Mar 2009 10:41:22 +0100 2287650 http://www.medworm.com/index.php?rid=2287651&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb7v36u767564085j%2F Abstract  Alopecia areata (AA) is regarded as a tissue-specific autoimmune disease for which several therapies have been suggested to modify the immune reaction against HFs, such as contact immunotherapy, psoralen plus ultraviolet A (PUVA), corticosteroids, cyclosporine, minoxidil, and dithranol. However, severe type AA, such as alopecia totalis (AT) and alopecia universalis (AU), often show resistance against these therapies. We applied a combination therapy with oral corticosteroid and oral PUVA for intractable cases of AT and AU. These patients took 20 mg/day corticosteroid and were irradiated with UVA on the whole body 2 h after taking methoxsalen for 1 month. In all patients, the terminal hair on the whole scalp regrew after 2 months. Two patients h... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2287651 Fri, 20 Mar 2009 10:41:20 +0100 2287651 http://www.medworm.com/index.php?rid=2287652&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fap80273x07851u60%2F This study offers an opportunity for understanding inflammation in psoriasis and supports the hypothesis that OPN could represent a potential target for therapeutic intervention in psoriatic patients. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-009-0939-5Authors Elisabetta Buommino, Second University of Naples Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology Naples ItalyMaria Antonietta Tufano, Second University of Naples Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology Naples ItalyNicola Balato, University of Naples Federico II Dermatology Clinic, Department of Systematic Pathology via Pansini 5 80131 Naples ItalyNunzia Canozo, Second University of Naples Department of Experimental... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2287652 Fri, 20 Mar 2009 10:41:19 +0100 2287652 http://www.medworm.com/index.php?rid=2276794&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn634135l28h34323%2F Abstract  The biosynthesis of retinoic acid (RA) from retinol is controlled by several enzymes, e.g. dehydrogenases (RalDH2, RoDH-4) and retinol-esterifying enzyme (LRAT), whereas its degradation mainly involves CYP26 enzymes. In keratinocytes, RA activates the nuclear retinoid-receptors inducing the transcription of many genes. Here, we examined the effects of RA and the CYP26 inhibitors, liarozole and talarozole, on retinoid metabolism and RA-regulated genes in organotypic epidermis. RA induced the expression of CYP26 enzymes already after 8 h, whereas LRAT exhibited a later response and peaked at 48 h, indicating a feedback induction of retinol esterification. In line with a reduced biosynthesis of RA from retinol after exogenous RA, the expression of RDH16 redu... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2276794 Wed, 18 Mar 2009 07:04:17 +0100 2276794 http://www.medworm.com/index.php?rid=2276795&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm028704mg4450h15%2F Abstract  The essential role played by CD25+CD4+ regulatory T (Treg) cells in the control of immunity against some pathogens such as Helicobacter pylori is now well established. But their role in cutaneous fungal infections is still unknown. Onychomycosis is the chronic fungal infection of the nails, which is very common. The aim of this study was to evaluate possible relationship of CD4+CD25+ Treg cells and onychomycosis. Peripheral blood samples were investigated for CD4+CD25+ Treg cells using flow cytometry analysis in 43 toenail onychomycosis patients and in 30 healthy controls. We have found that onychomycosis patients had a higher expression of CD25+CD4+ Treg cells than controls, with values of 8.45 ± 4.47% versus 4.64 ± 1.59%,... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2276795 Wed, 18 Mar 2009 07:04:16 +0100 2276795 http://www.medworm.com/index.php?rid=2276796&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd656284u42523607%2F Abstract  A first generation polyvalent vaccine (AS1001) was manufactured with protein from several cultured leishmania species, which proved to be effective in the treatment of psoriasis. To determine the effective factor, a single blind trial with four monovalent second generation vaccines (AS1002) was done in 26 subjects, which also resulted in remission of psoriasis. AS1002 vaccines were further purified, resulting in seven chromatography fractions (AS200) per species. In vitro testing of the fractions on blood lymphocytes resulted in subjects being categorized as low or high responders before treatment. Both responder groups had no statistical difference in clinical outcome after AS1001 treatment. Subsequently, a single-blind trial in 55 subjects treated with AS200 fracti... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2276796 Wed, 18 Mar 2009 07:04:15 +0100 2276796 http://www.medworm.com/index.php?rid=2263293&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F73126r7731841552%2F In this study, a cell-based assay system was developed to monitor TGF-β1 promoter activity and then used to evaluate the effects of activated TGF-β1 promoter in human epidermal keratinocytes (HaCaT). To accomplish this, a pMetLuc-TGF-β1 promoter plasmid that expresses the luciferase reporter gene in response to TGF-β1 promoter activity was constructed. Treatment of HaCaT with dihydrotestosterone, which is known to be a primary factor of AGA, resulted in a concentration-dependent increase in TGF-β1 promoter activity. However, treatment of HaCaT with the TGF-β1 inhibitor, curcumin, resulted in a concentration-dependant decrease in TGF-β1 expression. Subsequent use of this assay system to screen TGF-β1 revealed that HaCaT that were treated with apigenin showed decreased levels o... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2263293 Wed, 11 Mar 2009 15:12:06 +0100 2263293 http://www.medworm.com/index.php?rid=2248601&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw37j443mr6818255%2F Abstract  Since the symptoms of psoriasis may be changed by treatment with selective serotonin reuptake inhibitors (SSRIs), the expression of serotonin (5-HT) and its transporter protein (SERT) in the skin of patients with psoriasis were examined employing a biotinylated-streptavidine procedure. In biopsies of such skin staining for 5-HT was limited to platelets; the expression of SERT in the keratinocytes of involved regions was redistributed; the numbers of SERT-positive dendritic or round mononuclear cells in the epidermis of involved psoriatic skin were higher than in normal healthy control skin; and the dermis of the involved skin contained higher numbers of round inflammatory cells immunostained for SERT than either non-involved psoriatic skin or normal skin. Double-i... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2248601 Thu, 05 Mar 2009 14:59:37 +0100 2248601 http://www.medworm.com/index.php?rid=2226562&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa31936710666q213%2F Abstract  Application of hyaluronan (HA) containing cosmetic products to the skin is reported to moisturize and restore elasticity thereby achieving an antiwrinkle effect. In the skin, HA can be synthesized by dermal fibroblasts and N-acetylglucosamine (NAG) is a precursor for HA biosynthesis in the body. To study the effects of exogenous NAG on HA production in human dermal fibroblasts, HA production and HA-synthesizing enzymes 1, 2 and 3 mRNA expression in cultured human dermal fibroblasts were measured by ELISA and RT-PCR, respectively. The results showed that NAG promoted HA production while had no effect on the expression of HA-synthesizing enzymes 1, 2 and 3 mRNA in human dermal fibroblasts. Content Type Journal ArticleCategory Short CommunicationDOI 10.1007/s00403-0... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2226562 Fri, 27 Feb 2009 13:52:34 +0100 2226562 http://www.medworm.com/index.php?rid=2221426&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fur2ku7v2p6h123r8%2F Content Type Journal ArticleCategory ErratumDOI 10.1007/s00403-009-0934-xAuthors Jianyong Fan, Forth Military Medical University Dermatology Center of Chinese PLA, XiJing Hospital 710032 Xi’an ChinaZhu Shen, Third Military Medical University Department of Dermatology, Southwest Hospital 400038 Chongqing ChinaGang Wang, Forth Military Medical University Dermatology Center of Chinese PLA, XiJing Hospital 710032 Xi’an ChinaHuilan Yang, The General Hospital of Guangzhou Military Command Department of Dermatology 510010 Guangzhou ChinaYufeng Liu, Forth Military Medical University Dermatology Center of Chinese PLA, XiJing Hospital 710032 Xi’an China Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2221426 Thu, 26 Feb 2009 10:33:22 +0100 2221426 http://www.medworm.com/index.php?rid=2215575&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fmk6415326363g556%2F Abstract  Dermal papilla (DP) at the hair follicle base is important for hair growth. Recent studies demonstrated that mouse vibrissa DP cells can be cultured in the presence of fibroblast growth factor-2 (FGF-2), but lose expression of versican and their follicle-inducing activity during the culture, and that activation of the Wnt signal, which is inhibited by glycogen synthase kinase-3 (GSK-3), in the DP cells promotes hair growth activity. We therefore investigated the influence of a GSK-3 inhibitor, (2′Z,3′E)-6-bromoindirubin-3′-oxime (BIO), on the growth of human DP cells and mouse vibrissa follicles in culture. We first demonstrated that, similarly to mouse DP cells, human DP cells were able to be cultured up to 15 passages in the presence of FGF-2, and lost the... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2215575 Tue, 24 Feb 2009 09:41:58 +0100 2215575 http://www.medworm.com/index.php?rid=2152437&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk3lu77l716712151%2F Abstract  Aquaporin-3 (AQP3) is a water/glycerol transporting protein expressed strongly at the plasma membrane of keratinocytes. There is evidence for involvement of AQP3-facilitated water and glycerol transport in keratinocyte migration and proliferation, respectively. Here, we investigated the involvement of AQP3 in keratinocyte differentiation. Studies were done using AQP3 knockout mice, primary cultures of mouse keratinocytes (AQP3 knockout), neonatal human keratinocytes (AQP3 knockdown), and human skin. Cells were cultured with high Ca2+ or 1α,25-dihydroxyvitamin D3 (VD3) to induce differentiation. The expression of differentiation marker proteins and differentiating responses were comparable in control and AQP3-knockout or knockdown keratinocytes. Topical application... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2152437 Sat, 31 Jan 2009 07:19:41 +0100 2152437 http://www.medworm.com/index.php?rid=2149219&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk192812180098700%2F Abstract  Irradiation by ultraviolet A (UVA) initiates the suppression of skin contact hypersensitivity. However, the change in the whole body immunity by UVA irradiation of the eye is still unknown. The mice used in this study were separated into four groups namely: a control, UVA irradiation of the eye, UVA irradiation of the ear, UVA irradiation of the eye + a glucocorticoid receptor antagonist (RU-486) administrated, UVA irradiation of the eye with an adrenalectomy and non-irradiation + cortisol administrated groups. The eye or ear was locally exposed to UVA after covering the remaining body surface with aluminum foil at a dose of 110 kJ/m2 using a FL20SBLB-A lamp. Plasma adrenocorticotropic hormone, cortisol, and interleukin-10 (IL-10) content i... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2149219 Fri, 30 Jan 2009 07:12:12 +0100 2149219 http://www.medworm.com/index.php?rid=2107291&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr17155466280u465%2F Abstract  The conclusions that may be drawn by interpreting the current literature on the efficacy of photo(chemo)therapy in the treatment of atopic dermatitis (AD) are limited by several factors including publication bias, small sample sizes, high variability of parameters used in different studies, and in particular the lack of randomized controlled trials comparing different photo(chemo)therapeutic modalities. The newer ultraviolet (UV) modalities, such as medium-dose UVA1 and narrowband (NB) UVB, with a high output and a narrow emission spectrum may be considered the probably most efficacious regimens for treating acute and chronic AD, respectively, in particular when compared to conventional broadband UV regimens. There are no prospective trials on AD patients comparing... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2107291 Wed, 14 Jan 2009 07:08:44 +0100 2107291 http://www.medworm.com/index.php?rid=2086460&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu1x3348763261718%2F Content Type Journal ArticleCategory Letter to the EditorDOI 10.1007/s00403-008-0925-3Authors Larisa Prpić-Massari, Clinical Hospital Center Rijeka Department of Dermatovenerology Krešimirova 42 Rijeka CroatiaMarija Kaštelan, Clinical Hospital Center Rijeka Department of Dermatovenerology Krešimirova 42 Rijeka Croatia Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2086460 Sat, 03 Jan 2009 06:52:45 +0100 2086460 http://www.medworm.com/index.php?rid=2086459&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4p178468t631t23g%2F Abstract  Melanogenesis is a physiological process that results in the synthesis of melanin pigments, which play a crucial protective role against skin photocarcinogenesis. The present study was designed to determine the effects of 6-benzylaminopurine (6-BAP) on melanogenesis and elucidate the molecular events of melanogenesis induced by 6-BAP. To elucidate the pigmenting effect of 6-BAP and its mechanism, several experiments were performed in B16 melanoma cells. Melanin content, tyrosinase activity, cAMP production, and Western blots for proteins which are involved in melanogenesis were introduced in this study. Melanin content and tyrosinase activity increased in response to treatment with 6-BAP in a concentration-dependent manner. The tyrosinase, TRP-1, TRP-2 and MITF pr... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2086459 Sat, 03 Jan 2009 06:52:45 +0100 2086459 http://www.medworm.com/index.php?rid=2072830&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw521416773502356%2F Abstract  This investigation is a follow-up to our previous in vivo studies revealing that rapid stretch increases tissue insulin in murine skin flaps, coincident with the up-regulation of key angiogenic effectors and enhanced vascularization. In the present study, we used human umbilical vein endothelial cells (HUVECs) as an in vitro model system to determine the role of insulin in the chemical signals regulating the processes of proliferation and viability (survival). MTT-based colorimetric methods demonstrated that insulin enhances proliferation and survival of HUVECs. Western blot analysis revealed that protein kinase B (pAkt [Thr308]) and vascular endothelial growth factor (VEGF) were the insulin-responsive intermediates in proliferating endothelial cells (ECs). In ins... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2072830 Tue, 30 Dec 2008 07:07:56 +0100 2072830 http://www.medworm.com/index.php?rid=2072831&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F15kr4470450h2462%2F In conclusion, this COMT gene polymorphism may have contributed to the etiology of vitiligo in our Chinese population. Larger population-based studies are required to verify our findings. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-008-0920-8Authors Kai Li, Fourth Military Medical University Department of Dermatology, Xijing Hospital Xi’an 710032 ChinaChunying Li, Fourth Military Medical University Department of Dermatology, Xijing Hospital Xi’an 710032 ChinaLin Gao, Fourth Military Medical University Department of Dermatology, Xijing Hospital Xi’an 710032 ChinaLi Yang, Fourth Military Medical University Department of Dermatology, Xijing Hospital Xi’an 710032 ChinaMiao Li, Fourth Military Medical University Department of Dermatology, Xijing Hospital ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2072831 Sun, 28 Dec 2008 07:07:49 +0100 2072831 http://www.medworm.com/index.php?rid=2064723&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh8473wx441752423%2F Abstract  A growing body of evidence suggests that optimal induction of systemic anti-tumor immunity requires priming of both the CD4+ and CD8+ T cells that are specific for tumor-associated antigens (TAA). Recently, it was shown that MHC class II positive tumor cells are able to induce tumor-specific CD4+ T cells, and that this event may improve clinical outcome. This has rekindled the interest in modulating MHC class II expression in nonprofessional antigen presenting tumor cells. The class II transactivator (CIITA) is a major regulator of MHC class I and class II expression. We compared, in head and neck cancer cell lines, the effect of stable overexpression of CIITA to treatment with IFN-γ on the cell surface expression profile of MHC class I and II molecules. Here, we... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2064723 Tue, 23 Dec 2008 07:31:44 +0100 2064723 http://www.medworm.com/index.php?rid=2013095&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa88kq7w22324n1t1%2F Abstract  Chronic wounds are a major secondary complication for many people with impaired mobility. Electrical stimulation (ES) has been recommended as a adjunctive therapy, however optimal treatment paradigms have not been established. Our group seeks to determine the basic mechanisms underlying ES wound therapy, an area where understanding is currently limited. A feasibility study was carried out to develop the Ahn/Mustoe lapine wound model for systematic investigation of the effects of electrical stimulation on ischemic wound therapy. A standardized surgical procedure incorporated a hybrid stimulation system comprising an implantable mini-stimulator and surface electrodes, with creation of repeatable ischemic wounds. Twenty mature male New Zealand white rabbits (3 k... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2013095 Wed, 03 Dec 2008 08:26:32 +0100 2013095 http://www.medworm.com/index.php?rid=2004439&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm173773702v06485%2F Abstract  Leprosy is a disease caused by Mycobacterium leprae that initially affects the peripheral nervous system with patients exhibiting contrasting clinical, immunological, and pathological manifestations despite minimal genetic variation among bacilli isolates. Its clinical manifestations are related to M. leprae survival, innate and acquired immune responses, and interactions between host and bacterial proteins, preventing their invasion and infection, or promoting their development and pathogenesis. The complex molecular interactions in affected individuals influenced by the pathogenetic background will be explored in this review. However, the great genetic diversity imposes difficulty for understanding disease development, and it is likely that many factors and metabo... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=2004439 Sun, 30 Nov 2008 07:07:38 +0100 2004439 http://www.medworm.com/index.php?rid=1995599&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm615074273336332%2F In conclusion, the +49G allele is associated with increased risk of PTM in patients with GO. Studies with larger sample sizes are needed to confirm the results of the present study. Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00403-008-0919-1Authors Omid Khalilzadeh, Medical Sciences/University of Tehran Molecular Immunology and Immunogenetics Research Center Tehran IranHoda Mojazi Amiri, Medical Sciences/University of Tehran Molecular Immunology and Immunogenetics Research Center Tehran IranMaryam Tahvildari, Medical Sciences/University of Tehran Molecular Immunology and Immunogenetics Research Center Tehran IranMehdi Anvari, Medical Sciences/University of Tehran Molecular Immunology and Immunogenetics Research Center Tehran IranAlireza Esteghamati, Medical Sci... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1995599 Thu, 27 Nov 2008 09:20:57 +0100 1995599 http://www.medworm.com/index.php?rid=1963564&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa53331842178313t%2F Content Type Journal ArticleDOI 10.1007/s00403-008-0916-4 Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1963564 Fri, 14 Nov 2008 07:59:45 +0100 1963564 http://www.medworm.com/index.php?rid=1963565&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr1v2n6572528060j%2F Abstract  Gene therapy for a variety of human cancers containing the mutant p53 (mt-p53) gene has been performed by direct injection of a retroviral or adenoviral vector containing the wild-type p53 (wt-p53) gene. Because many individuals with skin squamous cell carcinoma (SCC) have been shown to carry the p53 gene mutation, these patients are candidates for p53 gene therapy. For this reason, we established ponasterone A-inducible the wild-type p53 (wt-p53) protein-expressing clones by transfecting a ponasterone-inducible vector containing the wt-p53 gene into HSC-1 cells, which harbor the mutated p53 m/w at codon 173 (GTG → TTG in one allele). Upon the induction of the wt-p53 protein, severe growth suppression was observed. Based on the results of the expression p... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1963565 Fri, 14 Nov 2008 07:59:41 +0100 1963565 http://www.medworm.com/index.php?rid=1944728&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fbt57715x23rt743t%2F Abstract  Natural CD4+CD25+ regulatory T cells (Treg) show a potent immunosuppressive function and contribute to immunologic self-tolerance by suppressing potentially auto-reactive T cells. Depletion of these cells leads to the induction of severe autoimmune diseases in animal models; more recently, several studies have also reported an impairment of Treg number and/or function in various human autoimmune diseases. For example, aberrant numbers of circulating CD4+CD25+ Treg have been seen in patients with type I diabetes, mycosis fungoides, graft-versus-host-reaction, and rheumatoid arthritis. Moreover, increased numbers of functionally active CD4+CD25+ Treg have been detected in the synovial fluid of patients with rheumatoid arthritis. In systemic lupus erythematosus (SLE), c... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1944728 Thu, 06 Nov 2008 00:34:06 +0100 1944728 http://www.medworm.com/index.php?rid=1944729&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft231140x7k307h71%2F This study suggests that the risk of certain cancers, particularly that of the digestive tract, in first-degree relatives of BCC-patients is increased. These findings may indicate a genetic predisposition to both skin and visceral malignancies in this patient group. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00403-008-0913-7Authors M. M. van Rossum, Radboud University Nijmegen Medical Centre Department of Dermatology P.O. Box 9101 6500 HB Nijmegen The NetherlandsD. Wopereis, Radboud University Nijmegen Medical Centre Department of Dermatology P.O. Box 9101 6500 HB Nijmegen The NetherlandsT. Hoyer, Radboud University Nijmegen Medical Centre Department of Dermatology P.O. Box 9101 6500 HB Nijmegen The NetherlandsI. Soerjomataram, Erasmus Medical Centre Department ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1944729 Thu, 06 Nov 2008 00:34:05 +0100 1944729 http://www.medworm.com/index.php?rid=1929669&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2311481qg45r3367%2F Abstract  Serotonin is a monoamine acting as a neuromediator in the central and peripheral nervous system. Recently, serotonin has also been shown to influence T- and B-cell function. The serotonin transporter is central in the regulation of the serotonergic system and widely expressed on cells of the immune system. A functional length polymorphism in the promoter of the serotonin transporter gene (5-HTTLPR) has been implicated in the genetic background of depression. Psoriasis is a complex disease with a polygenetic inheritance. In light of the role of T-cell mediated inflammation in psoriasis and the increased prevalence of depression in psoriatic patients, we analyzed the 5-HTTLPR polymorphism in 309 patients with psoriasis vulgaris and 315 healthy control individuals. N... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1929669 Sat, 01 Nov 2008 07:00:05 +0100 1929669 http://www.medworm.com/index.php?rid=1929672&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fgn81217313621714%2F Abstract  Recently, we have reported that the pathophysiological features of dermatitis induced by the repeated application with Dermatophagoides farinae (Df) extract ointment in NC/Nga mice were similar to those observed in the patients with atopic dermatitis. In the present study, we first examined whether the application of Df in other mouse strains could induce dermatitis. The repeated application of Df body (Dfb) ointment to the barrier-disrupted back of ICR, C57BL/6, and Balb/c mice did not cause any apparent skin lesions, although transient increase in serum immunoglobulin E (IgE) levels during antigen application was observed. On the other hand, in NC/Nga mice, dermatitis scores and serum IgE levels increased remarkably, and then these changes sustained for at least ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1929672 Sat, 01 Nov 2008 07:00:04 +0100 1929672 http://www.medworm.com/index.php?rid=1929671&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F126m56j313183542%2F Abstract  Fluorescence diagnosis (FD) seems to be a promising method for the non-invasive detection of tumour boundaries. We aimed to investigate the diagnostic performance of conventional FD of basal cell carcinoma (BCC). Patients were included in this study when they had a skin lesion on the head which was clinically consistent with the diagnosis of BCC. FD was performed using a 20% 5-aminolaevulinic acid (ALA) cream. The FD tumour area was determined 3.5 h after the ALA application using the naked eye and a Wood lamp. The tumour margins were marked on the basis of FD. The lesions were then excised according to the FD-defined tumour margin. On histology, the tumour margins were checked whether the lesion was completely excised in accordance with FD. Twenty-eight BCCs... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1929671 Sat, 01 Nov 2008 07:00:04 +0100 1929671 http://www.medworm.com/index.php?rid=1929670&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhx0m4210w4375g0n%2F Abstract  Darier’s disease (DD) is an autosomal dominant genodermatology. Mutations in the ATP2A2 gene encoding sarco-endoplasmic reticulum calcium pumping ATPase type 2 (SERCA2) have been identified as the molecular basis of DD. The aim of this study was to report two Chinese pedigree of DD and to explore the genetic mutations. Polymerase chain reaction was carried out to amplify the exons and flanking intron boundaries of the ATP2A2 gene followed by direct sequencing. Two novel missense mutations were identified, a change of C203 to A (A68E) in exon 3 was found in one family and a change of C2759 to T (S920F) in exon 19 in the other, which were located within the transmembrane domain of SERCA2, highly conserved during evolution. The A68E and S920F mutations might be regar... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1929670 Sat, 01 Nov 2008 07:00:04 +0100 1929670 http://www.medworm.com/index.php?rid=1929673&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F05n27590n7682717%2F Abstract  The influences of chronic UVB exposure on epidermal differentiation have been poorly studied compared to dermal photo-aging although those effects are very important in terms of photo-damage to the skin. The purpose of this study was to investigate the effects of chronic UVB exposure on keratin expression in the epidermis. The effects on murine skin of chronic exposure to weak UVB (below 1 MED) was examined by immunoblotting for keratins K10, K5, K6, and K16, by immunohistochemistry using antibodies to K6, K16, and Ki67 as well as by conventional HE staining of skin sections. Alterations of keratin expression induced by the chronic UVB exposure were distinct from those elicited by a single acute UVB exposure. The expression of keratins K6 and K16 was quite long-la... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1929673 Sat, 01 Nov 2008 07:00:03 +0100 1929673 http://www.medworm.com/index.php?rid=1900348&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx73k1717wk7337l3%2F Abstract  Human malignant melanoma is notoriously resistant to currently available pharmacological modulation. Our aim was to evaluate the anti-tumor effect of a novel synthetic retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carbo-xylic acid (CD437) on melanoma cell line A375. Analysis of cell morphology showed that CD437 promoted marked apoptosis in A375 cells. To explore the mechanisms of CD437-induced apoptosis, an NF-κB-luciferase reporter assay was performed, demonstrating that apoptosis induction by CD437 required activation of transcription factor NF-κB. Importantly, based on the findings that RIG-I (retinoic acid inducible gene I) can be induced by retinotic acid and can activate NF-κB through a CARD-containing adaptor protein VISA, we proposed a hypo... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1900348 Tue, 21 Oct 2008 06:12:07 +0100 1900348 http://www.medworm.com/index.php?rid=1900347&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7017276255u70361%2F Abstract  We have got the humanized antibody with high affinity and specificity against keratin by phage antibody library technology. To improve protein yields and get high affinity and specific anti-keratin antibody, we chose to express it in Pichia pastoris. Anti-keratin ScFv gene from plasmid p3MH/ScFv was subcloned into vector pPIC9K. After confirmed by DNA sequence analysis, the recombinant plasmid pPIC9K/ScFv was transduced into the genome of GS115 P. pastoris. Muts multiple insert transformants were screened by G418 and induced by 5 mL/L methanol to express soluble ScFv. After 6 days of methanol induction, anti-keratin ScFv was efficiently secreted into the medium. Western blot and ELISA assay proved the expressed protein had specific keratin-binding activity... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1900347 Tue, 21 Oct 2008 06:12:07 +0100 1900347 http://www.medworm.com/index.php?rid=1900349&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe686554342h84784%2F Abstract  The perifollicular vasculature undergoes hair-cycle dependent expansion and degeneration. Multiple soluble factors derived from dermal papilla cells (DPCs) may act on surrounding blood vessels to influence angiogenesis, growth and differentiation, and thereby regulate cyclic hair growth. The goal of this study was to examine the expression of angiogenin, a potent angiogenic factor, in human DPCs, and to determine its role in hair growth. Reverse transcription polymerase chain reaction (RT-PCR), western blotting, immunofluorescence and ELISA analyzes were used to investigate the expression of angiogenin in human DPCs, while semi-quantitative RT-PCR was used to assess angiogenin mRNA expression in murine skin phased at different stages of the hair cycle. We detected... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1900349 Tue, 21 Oct 2008 06:12:06 +0100 1900349 http://www.medworm.com/index.php?rid=1875534&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu44752g7846r2883%2F Abstract  In a recent study, we showed that long-term treatment with two different moisturizers affected TEWL in opposite directions. Therefore, we decided to examine the effect of these moisturizers on the cellular and molecular level. In a randomized controlled study on 20 volunteers, epidermal mRNA expression of genes essential for keratinocyte differentiation and desquamation after a 7-week treatment with two moisturizers was analyzed. Treatment with one test moisturizer increased gene expression of involucrin, transglutaminase 1, kallikrein 5, and kallikrein 7, while the other moisturizer affected only expression of cyclin-dependent kinase inhibitor 1A. Thus, moisturizers are able to modify the skin barrier function and change the mRNA expression of certain epidermal g... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1875534 Sat, 11 Oct 2008 11:17:09 +0100 1875534 http://www.medworm.com/index.php?rid=1867797&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy51gh8756364x74w%2F Abstract  Vitiligo is a common skin and hair depigmentary disorder that results from selective destruction of melanocytes. It occurs in a typical multifactorial, polygenic inheritance. Several studies have indicated that vitiligo is associated with some autoimmune diseases. In this paper we examined 6,516 vitiligo patients including clinical characteristics, familial involvement, and their association with other autoimmune diseases. Compared with sporadic vitiligo probands, familial vitiligo probands have earlier age onset and longer disease duration. The prevalences of four autoimmune diseases namely rheumatoid arthritis, chronic urticaria, alopecia areata and psoriasis, were significantly elevated in generalized vitiligo probands and their first-degree relatives. The prev... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1867797 Wed, 08 Oct 2008 09:50:19 +0100 1867797 http://www.medworm.com/index.php?rid=1843982&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp5331m28291n61uh%2F Abstract  Upregulation of TNF-α is a key early response to ultraviolet B (UVB) by keratinocytes (KCs), and represents an important component of the inflammatory cascade in skin. UVB irradiation induces TNF-α expression in both KCs and dermal fibroblasts, with TNF-α mRNA induction seen as early as 1.5 h after UVB. We previously reported that the effects are wavelength-specific: TNF-α expression and secretion are induced by UVB (290–320 nm), but not by UVA (320–400 nm). Moreover, we found that IL-1α, a cytokine also present in irradiated skin, substantially and synergistically enhances the induction of TNF-α by UVB, and the induction of TNF-α by this combination of UVB with IL-1α is mediated through increased TNF-α gene transcription. We investigate... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1843982 Tue, 30 Sep 2008 09:04:00 +0100 1843982 http://www.medworm.com/index.php?rid=1843981&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F010g964237m10000%2F Content Type Journal ArticleCategory AbstractsDOI 10.1007/s00403-008-0905-7 Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1843981 Tue, 30 Sep 2008 09:04:00 +0100 1843981 http://www.medworm.com/index.php?rid=1843984&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft1271t3x238740mw%2F In this study, human and murine hair follicles were treated with RXM in order to find the possibility to cure hair loss disease such as androgenetic alopecia (AGA). In AGA, dihydrotestosterone signals apoptosis in dermal papilla cells in susceptible individuals, resting in premature termination of anagen and early entry into catagen. Therefore, anti-apoptotitic drug has a possibility of new candidate for AGA. This study revealed RXM antagonized the in vitro inhibitory effect of IFN-γ on proliferation of keratinocytes and induction of apoptosis in murine and human hair bulb. RXM increases hair elongation and inhibits catagen-like changes induced in vitro with IFN-γ in murine and human hair follicles. Furthermore, topical 5% RXM solution effectively restores hair growth in about hal... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1843984 Mon, 29 Sep 2008 20:19:05 +0100 1843984 http://www.medworm.com/index.php?rid=1843983&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb714125h10r6445r%2F Abstract  Vitiligo is a common skin disorder that is caused by selective destruction of melanocytes, resulting in disfiguring loss of pigment. There are convincing evidences that cytokines and T cell mediated immunity may have a role in its pathogenesis. Given the fact that cytokine production is under genetic control, in this study, we have investigated IFN-γ +874 T/A and TNF-α −308 G/A gene polymorphisms in a total of 176 vitiligo patients and 545 controls. IFN-γ +874 T/A and TNF-α −308 G/A gene polymorphisms were genotyped via Allele Specific Oligonucleotide PCR (ASO-PCR) method. The results showed that the TNF-α −308 G/A polymorphism was more common in vitiligo patients than controls (P = 0.0004). This difference was only significant between female p... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1843983 Mon, 29 Sep 2008 20:19:05 +0100 1843983 http://www.medworm.com/index.php?rid=1843986&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2826082637434551%2F Abstract  Paraneoplastic pemphigus patients (PNP) develop a group of autoantibodies, among which those against envoplakin and periplakin are almost always found. Epitope mapping has indicated that the linker subdomains of the proteins harbor the major antigenic sites recognized by PNP sera. In order to detect specific autoantibodies for the diagnosis of PNP, we expressed recombinant proteins containing linker subdomains of human periplakin and envoplakin in a human kidney cell line, and used them as the antigens for ELISAs. We found that all of the sera from 16 PNP patients recognized these two recombinant proteins by ELISA, and sera from 20 pemphigus vulgaris (PV), 12 pemphigus foliaceus (PF), 20 bullous pemphigoid (BP), 2 Castleman’s tumor without PNP and 20 normal cont... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1843986 Mon, 29 Sep 2008 20:19:04 +0100 1843986 http://www.medworm.com/index.php?rid=1843985&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F24221771568j879r%2F Abstract  Autosomal recessive hypotrichosis simplex (ARHS) manifests with paucity of hair appearing during early childhood. We assessed four affected families. We initially genotyped three of these families for a panel of microsatellite markers spanning all ARHS-associated loci and obtained data suggesting linkage to 3q27, encompassing LIPH, which had previously been shown to be associated with ARHS. Accordingly, a homozygous duplication mutation in exon 2 of this gene (c.280_369dup; p.Gly94_Lys123dup) was found to segregate with the disease in all the families. Through the identification of the first duplication mutation in the human LIPH gene, we provide further evidence supporting a role for the phospholipase signalling pathway in hair growth and differentiation. Conten... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1843985 Mon, 29 Sep 2008 20:19:04 +0100 1843985 http://www.medworm.com/index.php?rid=1832509&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F00274475m13681u6%2F Abstract  Autoimmune diseases can be preceded by a symptom-free phase which is defined by the presence of autoantibodies, and may last for many years. These autoantibodies may have a high positive predictive value for disease onset, severity and organ-specific complications, especially in genetically prone individuals. Characteristic autoantibodies and susceptible genes have been identified in many autoimmune systemic and mucocutaneous diseases such as systemic lupus erythematosus, pemphigus, vitiligo, dermatitis hepretiformis and even psoriasis. Prevention of overt disease may be achieved once high-risk individuals are identified and triggering factors are avoided. Numerous environmental factors, such as vitamin D deficiency, ultraviolet light, smoking, drugs, etc., that m... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1832509 Thu, 25 Sep 2008 07:21:23 +0100 1832509 http://www.medworm.com/index.php?rid=1810974&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft3017010p725t028%2F In this study, CPN cases were retrospectively collected from multiple Japanese clinics, and analyzed for detailed clinical and histopathological manifestations, in order to redefine the clinical entity of CPN and to propose appropriate diagnostic criteria for CPN and PN. According to the CPN description in Rook’s Textbook of Dermatology, we collected 22 cases with appropriate histopathological findings. Of the 22 cases, none progressed to PN or death during the follow-up period, 32% had peripheral neuropathy and 27% had myalgia. Regarding extracutaneous symptoms with CPN, 17 dermatological specialists in vasculitis sustained the opinion that CPN can be accompanied by peripheral neuropathy and myalgia but these symptoms are limited to the same area as skin lesions. Based on these r... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1810974 Fri, 19 Sep 2008 06:30:33 +0100 1810974 http://www.medworm.com/index.php?rid=1805639&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh3762881w4578552%2F Abstract  Psoriasis is a disease presenting cutaneous, immunological and vascular abnormalities. Oral cyclosporin therapy has been shown to be effective for the disease. Clinical and laboratory findings affecting the response of oral cyclosporin therapy in patients with psoriasis were studied. Forty-seven patients with psoriasis (male:female = 27:20, age 56.7 + 12.6 years) were studied. The response to oral cyclosporin therapy was categorized as excellent, good, fair and poor according to decrease of PASI score and decrease of cyclosporin dose. Clinical and laboratory findings including cyclosporin trough level and high sensitivity-CRP were statistically analyzed. Nine patients showed excellent response, 17 good response, 19 fair response and 2 poor ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1805639 Wed, 17 Sep 2008 06:55:14 +0100 1805639 http://www.medworm.com/index.php?rid=1805638&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr324hv28274h762v%2F Abstract  Genetic differences are involved in the development of lupus erythematosus (LE). Skin lesions are influenced by environmental triggers such as ultraviolet light, temperature, and chemical stresses, and the patterns of skin lesion are variable in cutaneous LE such as systemic LE (SLE), chronic discoid LE (CDLE), subacute cutaneous LE (SCLE), and LE tumidus (LET). Although there are a few conflicting reports, many Japanese dermatologists feel there are photosensitivity differences in lupus erythematosus between Asian and Caucasian subjects with SCLE and LET. HLA studies in Japanese subjects revealed that HLA-DRB1*1501 association was with both CDLE and SLE. The association between HLA-Cw6 and CDLE was first reported in Japanese population, and a HLA-A33-B44-DRB1*1302... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1805638 Wed, 17 Sep 2008 06:55:14 +0100 1805638 http://www.medworm.com/index.php?rid=1805637&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F21186n68687m2223%2F Abstract  At least 71 patients have been reported in which their otherwise typical subacute cutaneous lupus erythematosus (SCLE) skin lesions were felt to have been temporally associated with the systemic administration of a drug. The mean age of this cohort of drug-induced SCLE (DI-SCLE) patients was 59 years of age which is somewhat older than the mean age of previously reported idiopathic SCLE patient cohorts. Patients had been taking the suspected triggering drug for weeks to years before the onset of SCLE skin lesions. In addition, it was not unusual for 2–3 months to be required for resolution of the SCLE skin lesions following discontinuation of the triggering drug. A relatively large number of drugs representing different pharmacological classes have bee... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=1805637 Wed, 17 Sep 2008 06:55:14 +0100 1805637