MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Archives of Dermatological Research' source. Wed, 08 Feb 2012 13:12:27 +0100 http://www.medworm.com/index.php?rid=5667276&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw50316725406jp84%2F Abstract  Anti-inflammatory therapeutic options for the topical treatment of skin diseases with inflammatory or allergic contribution are mostly limited to topical glucocorticoids and calcineurin inhibitors. Both compound classes induce adverse effects. Elevation of intracellular cyclic adenosine monophosphate (cAMP) by inhibition of phosphodiesterase 4 was shown to induce potent anti-inflammatory effects, but the safety profile of currently available compounds is not sufficient. A different approach to increase intracellular cAMP is the substitution of chemically stabilized cAMP analogues. Bucladesine is a stabilized cAMP analogue with an excellent safety profile which had been marketed as topical treatment of impaired wound healing. In the current study, a novel water fre... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5667276 Thu, 02 Feb 2012 18:09:59 +0100 5667276 http://www.medworm.com/index.php?rid=5659840&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fxt477p2m945x3506%2F Content Type Journal ArticleCategory ErratumPages 1-1DOI 10.1007/s00403-012-1211-yAuthors Genevieve M. Sadler, Department of Dermatology, Sir Charles Gairdner Hospital, Hospital Ave, Nedlands, WA 6009, AustraliaHilary J. Wallace, Burn Injury Research Unit, School of Surgery, University of Western Australia, Crawley, AustraliaMichael C. Stacey, School of Surgery, Fremantle Hospital, University of Western Australia, Fremantle, Australia Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5659840 Tue, 31 Jan 2012 16:45:56 +0100 5659840 http://www.medworm.com/index.php?rid=5638562&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh654178j5878717x%2F In this study, data collected on RHE exposed to well-characterized sensitizing chemicals, such as dinitrofluorobenzene, oxazolone, cinnamaldehyde and isoeugenol, revealed a transient expression of IL-8 mRNA in association with abundant IL-8 cell release. Investigations of keratinocyte signaling illustrate transient activation by tissue exposure to sensitizing chemicals of the epidermal growth factor receptor (EGFR). This activation of EGFR tyrosine kinase is involved in the expression and release of IL-8. The IL-8 release appears also to be partially dependent on p38 and ERK 1/2 MAPK activation. Moreover, data suggest that heparin-binding EGF-like growth factor (HB-EGF) expression and release induced after exposure of RHE to sensitizing chemicals are also under the control of EGFR ty... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5638562 Tue, 24 Jan 2012 07:30:34 +0100 5638562 http://www.medworm.com/index.php?rid=5638563&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F18021h812663841u%2F Abstract  Solar ultraviolet (UV) radiation is an important risk factor in skin carcinogenesis. This has been attributed mainly to the UVB waveband because the high-energetic photons are capable of interacting with DNA and inducing DNA damage. Recently, UVA light has also gained increasing interest in relation to DNA alteration. Although UVA photons are less energetic than UVB, they comprise a major fraction of sunlight UV radiation and penetrate deep into the skin. The study was carried out to compare the acute effects of UVA and UVB light on SKH-1 mice in relation to DNA damage and associated parameters. Mice were exposed to UVA (10 and 20 J/cm2) or UVB (200 and 800 mJ/cm2) radiation. The number of DNA single-strand breaks (SSB) in lymphocytes, amount of phosphory... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5638563 Tue, 24 Jan 2012 07:30:33 +0100 5638563 http://www.medworm.com/index.php?rid=5620188&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F78t12415238g1022%2F Abstract  Autosomal recessive congenital ichthyosis (ARCI) is a group of disorders characterized by abnormal desquamation of the skin and a disrupted epidermal water barrier. Ichthyin/NIPAL4 gene mutations have been identified in a subgroup of ARCI patients, but the role of ichthyin in epidermis remains elusive. In order to obtain new insights concerning the characteristics of ichthyin and the ARCI pathogenesis, we studied the expression and localization of ichthyin and related epidermal components in cultured keratinocytes and skin sections from patients with Ichthyin mutations and healthy controls. We observed an up-regulation of Ichthyin mRNA levels after in vitro differentiation of keratinocytes from both a patient with Ichthyin mutations and controls. Confocal and electr... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5620188 Thu, 19 Jan 2012 06:53:36 +0100 5620188 http://www.medworm.com/index.php?rid=5620189&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F17xj169744297575%2F Abstract  Prolactin (PRL) is well recognised for its role(s) in mammary gland development and function. Moreover, its role in skin biology, including the potent regulation of human hair growth, is becoming clearer. Less widely appreciated, however, is the potential role of PRL in the pathobiology of psoriasis. While the relationship between PRL and psoriasis remains enigmatic, several recent publications on the PRL–psoriasis connection have demonstrated a reawakening of interest in this conundrum. We take the occasion of these reports to underscore the importance of dissecting the role(s) of PRL in the aetiopathology of psoriasis, not least since this may help to identify novel hormonal treatment strategies in its management. Content Type Journal ArticleCategory News ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5620189 Tue, 17 Jan 2012 07:12:28 +0100 5620189 http://www.medworm.com/index.php?rid=5620190&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F0627x250630028wp%2F In this study, we evaluated the association between IgE seropositivity to the recombinant allergens Ani s 1 and Ani s 7 and several A. simplex-associated allergic disorders. Twenty-eight patients with GAA and 40 patients with CU+ were studied and their IgE responses were compared with a control group composed of patients with chronic urticaria not sensitized to A. simplex (CU−) according to the skin prick test, as well as a group of 15 healthy subjects not referring urticaria or currently A. simplex associated symptoms. 82.1% of GAA patients and 42.5% of CU+ patients were positive for Ani s 1 (P < 0.001), while the Ani s 7 allergen was recognized by 92.9 and 92.5% of sera from patients with GAA and CU+, respectively. The combined positivity obtained for both allergens rea... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5620190 Tue, 17 Jan 2012 07:12:25 +0100 5620190 http://www.medworm.com/index.php?rid=5581943&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff36555447846n055%2F Abstract  Autophagy is a tightly regulated physiological process essential for cellular maintenance, differentiation, development, and homeostasis. Aberration of this process associates with the pathogeneses of several diseases in mammals. Hypertrophic scar (HS) is characterized by an abundance of collagenous tissue with hypercellularity. However, the molecular mechanism in HS formation is poorly understood. We compared the autophagic capacity in HS and its normal skin (NS) counterparts and explored the molecular mechanism of autophagy during the formation of HS. Microtubule-associated protein 1 light chain 3 (LC3) proteins in HS and NS were detected by immunohistochemistry, Western blot and quantitative real-time PCR (qPCR). The data showed that LC3 positive staining in HS... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5581943 Wed, 11 Jan 2012 17:53:49 +0100 5581943 http://www.medworm.com/index.php?rid=5581944&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F71461mv652134316%2F Abstract  In recent times, there is widespread use of 2.45-GHz irradiation-emitting devices in industrial, medical, military and domestic application. The aim of the present study was to investigate the effect of 2.45-GHz electromagnetic radiation (EMR) on the oxidant and antioxidant status of skin and to examine the possible protective effects of β-glucans against the oxidative injury. Thirty-two male Wistar albino rats were randomly divided into four equal groups: control; sham exposed; EMR; and EMR + β-glucan. A 2.45-GHz EMR emitted device from the experimental exposure was applied to the EMR group and EMR + β-glucan group for 60 min daily, respectively, for 4 weeks. β-glucan was administered via gavage at a dose of 50 mg/kg/day befo... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5581944 Wed, 11 Jan 2012 17:53:48 +0100 5581944 http://www.medworm.com/index.php?rid=5560245&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh602874j77532354%2F Abstract  To evaluate the association between psoriasis and risk of diabetes, pertinent studies were identified by searching electronic databases and by reviewing the reference lists of retrieved articles. We included observational studies that examined the association between psoriasis and risk of diabetes. Two reviewers independently assessed eligibility and used a standardized form to collect data from published studies. The study quality was assessed by the Newcastle–Ottawa Scale. A total of 22 eligible studies that included 3,307,516 participants fulfilled the inclusion criteria. Compared to individuals without psoriasis, subjects with psoriasis had a 1.42-fold increased risk of diabetes (95% CI, 1.40–1.45). Findings from this meta-analysis suggest that individuals... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5560245 Sat, 31 Dec 2011 16:48:40 +0100 5560245 http://www.medworm.com/index.php?rid=5552407&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr1173q13n88l3336%2F In conclusion, low pseudoallergen diet helps to reduce the urticarial activity in CU. The change in urticarial activity correlates with the change in LT levels. Content Type Journal ArticleCategory Original ArticlePages 1-6DOI 10.1007/s00403-011-1203-3Authors Gulsen Akoglu, Department of Dermatology, Faculty of Medicine, Hacettepe University, P. O. 06100, Sihhiye, Ankara, TurkeyNilgun Atakan, Department of Dermatology, Faculty of Medicine, Hacettepe University, P. O. 06100, Sihhiye, Ankara, TurkeyBanu Çakır, Department of Public Health, Faculty of Medicine, Hacettepe University, Ankara, TurkeyOmer Kalayci, Pediatric Allergy and Asthma Unit, Faculty of Medicine, Hacettepe University, Ankara, TurkeyMutlu Hayran, Research Unit, Faculty of Medicine, Hacettepe University, Ankara, Turk... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5552407 Mon, 26 Dec 2011 16:46:02 +0100 5552407 http://www.medworm.com/index.php?rid=5544643&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F90q174tt022079xx%2F Abstract  The nervous system contributes to allergic contact dermatitis (ACD). Elucidation of the implication of the nervous system during different stages of ACD could be of therapeutic value. Our aim was to study the kinetics and contribution of the nervous system to ACD by investigating innervation and expression of neuropeptides in skin biopsies obtained at 0, 6, 24, 48 and 72 h post-challenge. Biopsies were stained using antisera against protein gene product (PGP) 9.5, growth associated protein (GAP)-43, substance P and its receptor (R) neurokinin (NK)-1, NKA and NK-2R, and calcitonin gene-related peptide (CGRP). GAP-43-immunoreactive (ir) nerves revealed a time-dependent increase that was more pronounced at 48 and 72 h, while PGP 9.5-ir nerves remained unalte... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5544643 Sat, 24 Dec 2011 06:41:41 +0100 5544643 http://www.medworm.com/index.php?rid=5538069&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ftk7754540621k269%2F Abstract  This pilot study investigated oral doxycycline as an adjunct to compression therapy for non-healing venous leg ulcers. Ten patients received doxycycline 20 mg twice daily (low-dose doxycycline) and ten patients received doxycycline 100 mg twice daily (high-dose doxycycline). Utilising a pre-test post-test study design, ulcer area was measured and wound fluid was collected before and after 4 weeks of treatment. In the high-dose doxycycline group, the reduction in median ulcer area was 48% (p = 0.1) and there was a significant reduction in wound fluid total matrix metalloprotease-1 (p = 0.02). These effects were not observed with low-dose doxycycline. There were no significant changes in wound fluid tumour necrosis factor-α or quant... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5538069 Tue, 20 Dec 2011 16:47:49 +0100 5538069 http://www.medworm.com/index.php?rid=5504977&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4276j7j253704252%2F The objective of the present study is to enhance the bioavailability of β-sitosterol by its complexation with phosphatidyl choline and then to formulate it as phyto-vesicles for the treatment of alopecia. The complex of β-sitosterol was prepared with phosphatidyl choline and characterized on the basis of solubility, melting point, TLC, UV, IR and NMR spectroscopy. This complex was then formulated as phyto-vesicles and then characterized. The results revealed that effect on alopecia is better in case of phyto-vesicles as compared to the complex, physical mixture and the β-sitosterol itself. Enhanced bioavailability of the β-sitosterol complex may be due to the amphiphilic nature of the complex, which greatly enhance the water and lipid solubility of the compound. The present study... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5504977 Mon, 12 Dec 2011 17:13:17 +0100 5504977 http://www.medworm.com/index.php?rid=5486946&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc2plu71056k18445%2F The objectives of this study are: evaluation of the association between PTGS2 −765G>C polymorphism and the occurrence of non-melanoma skin cancer, the relationship between this polymorphism and cyclooxygenase-2 activity in skin tissue, as well as the correlation with serum CRP levels in patients with non-melanoma skin cancer. We used PCR–RFLP technique to explore −765G>C PTGS2 gene polymorphism, colorimetric analysis for cyclooxygenase-2 activity in skin tissue and immunoturbidimetric assay for CRP serum levels in 174 patients with non-melanoma skin cancer [54 patients with basal cell carcinoma (BCC) and 120 patients with squamous cell carcinoma (SCC)] and 80 healthy subjects. PTGS2 −765G>C polymorphism failed to show an association with non-melanoma skin cancer risk... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5486946 Wed, 07 Dec 2011 12:54:48 +0100 5486946 http://www.medworm.com/index.php?rid=5486947&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2624k113r8u14x47%2F Abstract  Atopic dermatitis (AD) is a chronic inflammatory disease of the skin for which there are no reliable biomarkers to assess clinical severity. Serum interleukin-18 (IL-18) levels may be associated with AD severity. To identify putative biomarkers associated with clinical severity in adult AD patients, we enrolled 121 adult AD patients (mean age 35.7 years) and 50 healthy controls (mean age 31.7 years). We compared these groups for blood eosinophils and serum levels of IL-18, thymus and activation-regulated chemokine (TARC), total IgE, and lactate dehydrogenase (LDH). We also determined S. aureus enterotoxin B (SEB) specific IgE levels and the SCORingAD (SCORAD) scores for AD patients. For AD patients, stepwise logistic regression was used to estimate odds r... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5486947 Tue, 06 Dec 2011 17:01:17 +0100 5486947 http://www.medworm.com/index.php?rid=5486948&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg7g0408p53381695%2F Abstract  Generalized peeling skin syndrome (PSS) is a rare autosomal recessive dermatosis manifesting with continuous exfoliation of the stratum corneum. The inflammatory (type B) subtype of PSS was recently found to be caused by deleterious mutations in the CDSN gene encoding corneodesmosin, a major component of desmosomal junctions in the uppermost layers of the epidermis. In the present study, we assessed a 10-month-old baby, who presented with generalized superficial peeling of the skin. Using PCR amplification and direct sequencing, we identified the third PSS-associated mutation in CDSN, a homozygous 4 bp duplication in the second exon of the gene (c.164_167dup GCCT; p.Thr57ProfsX6). These data further support the notion that corneodesmosin deficiency impairs cel... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5486948 Tue, 06 Dec 2011 17:01:15 +0100 5486948 http://www.medworm.com/index.php?rid=5476665&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk1451g4025413686%2F In conclusion, these data highlight the possibility of miRNA-146a and miRNA-155 involvement in the regulation of the immune response in OLP. Content Type Journal ArticleCategory Original PaperPages 1-5DOI 10.1007/s00403-011-1197-xAuthors Telma Cristina Arão, Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilAndré Luiz Sena Guimarães, School of Dentistry, Universidade Estadual de Montes Claros, Belo Horizonte, MG, BrazilAlfredo Maurício Batista de Paula, School of Dentistry, Universidade Estadual de Montes Claros, Belo Horizonte, MG, BrazilCarolina Cavaliéri Gomes, Department of Pathology, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilRicardo Santiago Gomez, Department of Oral Surgery... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5476665 Sat, 03 Dec 2011 16:49:42 +0100 5476665 http://www.medworm.com/index.php?rid=5476666&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb162632g5k1160x1%2F Abstract  Platelet-activating factor acetylhydrolase (PAF-AH) has been demonstrated to be one of anti-inflammatory and anti-apoptotic factors, suggesting the potential to be involved in the development of systemic sclerosis (SSc). The aim of this study is to determine serum PAF-AH levels and their clinical associations in patients with SSc. Serum PAF-AH levels were examined by enzyme-linked immunosorbent assay in 57 patients with SSc and 24 healthy individuals. Serum PAF-AH levels were significantly elevated in SSc patients (130.4 ± 69.5 ng/ml) compared with healthy individuals (81.6 ± 34.8 ng/ml; P < 0.001). Among SSc patients, there were no differences in serum PAF-AH levels between those with diffuse cutaneous SSc (135.5 ±... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5476666 Fri, 02 Dec 2011 17:30:49 +0100 5476666 http://www.medworm.com/index.php?rid=5469077&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg874175835051258%2F Abstract  Since most first-generation antihistamines have undesirable sedative effects on the central nervous systems (CNS), newer (second-generation) antihistamines have been developed to improve patients’ quality of life. However, there are few reports that directly compare the antihistaminic efficacy and impairment of psychomotor functions. We designed a double-blind, placebo controlled, crossover study to concurrently compare the clinical effectiveness of promethazine, a first-generation antihistamine, and fexofenadine and olopatadine, second-generation antihistamines, by measuring their potency as peripheral inhibitors of histamine-induced wheal and flare. Further, we investigated their sedative effects on the CNS using a battery of psychomotor tests. When single the... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5469077 Wed, 30 Nov 2011 18:42:23 +0100 5469077 http://www.medworm.com/index.php?rid=5457867&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F05018v2u5rw82744%2F Abstract  The gene ATP2C1 is identified as the defective gene in Hailey–Hailey disease (HHD). The nonsense and missense are two common types of mutations and have ,respectively, been detected in many HHD patients. The aims of our study were to identify the pathogenic ATP2C1 abnormality in Chinese HHD patients, and to compare nonsense and missense mutations in vivo to provide further understanding of the molecular and the physiological basis of HHD. The nucleotide sequencing of the ATP2C1 gene was performed in HHD patients, unaffected family members and 100 unrelated individuals. Meanwhile, we detected and analyzed the clinical manifestations, the expression of ATP2C1 mRNA and hSPCA1 protein in the two types of mutations. Three heterozygous mutations were identified, inclu... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5457867 Mon, 28 Nov 2011 16:49:51 +0100 5457867 http://www.medworm.com/index.php?rid=5457868&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr561u963p88086wv%2F In conclusion, NF1 patients may present a mineral bone involvement, with vitamin D deficiency; calcium and vitamin D supplementation is necessary to restore these bone mineral metabolic alterations. Content Type Journal ArticleCategory Short CommunicationPages 1-7DOI 10.1007/s00403-011-1191-3Authors Luigi Petramala, Department of Internal Medicine and Medical Specialties, Secondary Hypertension Unit, University ‘‘Sapienza’’, Viale del Policlinico 155, 00161 Rome, ItalySandra Giustini, Department of Dermatology, University ‘‘Sapienza’’, Rome, ItalyLaura Zinnamosca, Department of Internal Medicine and Medical Specialties, Secondary Hypertension Unit, University ‘‘Sapienza’’, Viale del Policlinico 155, 00161 Rome, ItalyCristiano Marinelli, Department of Interna... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5457868 Sat, 26 Nov 2011 16:48:45 +0100 5457868 http://www.medworm.com/index.php?rid=5449858&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx13137q056637348%2F Abstract  Pigment epithelium-derived factor (PEDF), first purified from the conditioned medium of human retinal pigment epithelial cells, has been shown to be a highly effective inhibitor of angiogenesis. A recent study showed that PEDF is also a novel adipokine secreted mainly by adipocytes. Psoriasis has been shown to be associated with metabolic syndrome (MS) and some adipokines. Since PEDF levels were reported to be elevated in the serum of patients with MS, we examined PEDF levels in the serum of psoriasis patients and studied the relationships between PEDF levels and other cytokines. Circulating levels of PEDF were significantly elevated in the sera of psoriasis patients compared to normal controls. PEDF levels were highly negatively associated with TNF-α levels in n... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5449858 Fri, 25 Nov 2011 17:59:46 +0100 5449858 http://www.medworm.com/index.php?rid=5408200&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff0t5615k379gmt74%2F Abstract   Xeroderma pigmentosum (XP) is a rare disorder characterized by a high skin sun-sensitivity predisposing to skin cancers at an early age. Among Tunisian XP patients with an intermediate skin phenotype, 92% presented neurological abnormalities related to XPA gene deficiency. Clinical variability of the XP-A phenotype is associated with a mutational heterogeneity. In the present study, two Tunisian families with severe dermatological and neurological XP phenotypes were investigated in order to determine clinical characteristics and genetic basis. Two Tunisian families with four XP affected children were examined in the Dermatology Department. Clinical features showed severe presentation of the disease. Coding regions of the XPA gene were analysed by direct sequencing... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5408200 Fri, 11 Nov 2011 12:49:04 +0100 5408200 http://www.medworm.com/index.php?rid=5386883&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu55532027t4370nn%2F This study has shown some differences in microbiota between psoriasis and normal skin. Whether these are of primary aetiological significance, or secondary to the altered skin of psoriasis remains to be determined. Content Type Journal ArticleCategory Original PaperPages 1-8DOI 10.1007/s00403-011-1189-xAuthors Annika Fahlén, Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Solna, SwedenLars Engstrand, Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Solna, SwedenBarbara S. Baker, London, UKAnne Powles, Faculty of Medicine, Imperial College, Room G3, St Mary’s Campus, London, W2 1PG UKLionel Fry, Faculty of Medicine, Imperial College, Room G3, St Mary’s Campus, London, W2 1PG UK Journal Archives of Dermatological Research... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5386883 Mon, 07 Nov 2011 17:14:44 +0100 5386883 http://www.medworm.com/index.php?rid=5386884&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy043381025977103%2F Content Type Journal ArticleCategory ErratumPages 1-1DOI 10.1007/s00403-011-1188-yAuthors Rikke Zillmer, Department of Dermatology, Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, DenmarkHannah Trøstrup, Department of Dermatology, Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, DenmarkTonny Karlsmark, Department of Dermatology, Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, DenmarkPeter Ifversen, Global R&D, Coloplast A/S, Humlebæk, DenmarkMagnus S. Ågren, Department of Dermatology, Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Bispe... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5386884 Sat, 05 Nov 2011 17:10:06 +0100 5386884 http://www.medworm.com/index.php?rid=5386887&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F173q4401471g734n%2F We report finding a simple method to partially reproduce the characteristic process of molting that takes place in invertebrates using human skin explants in vitro. In this method, human skin explants discarded from regular plastic surgery procedures were kept, submersed, in regular growth medium for 10 days at 4°C. After that period, the skin explants were cultured at the air–liquid interface for another 10 days. Histological analysis of the skin revealed the formation of one full epidermal structure and an additional intermediate epidermal structure containing a putative stratum corneum, superimposed one of top of the other, in which we consider an equivalent model of “molting” or “ecdysis”. Basic analysis of cell proliferation and differentiation of the explan... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5386887 Sat, 29 Oct 2011 05:41:32 +0100 5386887 http://www.medworm.com/index.php?rid=5386886&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Frt0w568575804068%2F Abstract  A neutral cysteine protease, bleomycin hydrolase (BH), is widely expressed in mammalian tissues, with the skin seeming to contain the highest level. Our previous study revealed that BH transcription is modulated both during differentiation and by cytokines. However, BH involvement in keratinization disorder is not well known. In the present study, we performed immunohistochemical studies of BH and other serine/cysteine proteases in human normal skin and lesional skin with keratinization disorders. BH-positive cells were detected in granular layers of orthokeratotic and hyperkeratotic skin diseases, such as erythrokeratoderma and lichen planus. In parakeratotic skin diseases with porokeratosis, pityriasis rubra pilaris and psoriasis, BH staining was decreased in le... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5386886 Sat, 29 Oct 2011 05:41:32 +0100 5386886 http://www.medworm.com/index.php?rid=5386885&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F28q58u232186kn16%2F Abstract  Pemphigus is an autoimmune blistering disease characterized by severe and chronic course, histopathologically characterized by infiltration of a large quantity of eosinophils, neutrophils, and activated Th1 and Th2 cells around the blister. Polarization of Th cells to Th1 or Th2 phenotypes, a critical aspect of cell-mediated immunity, is influenced by production of early cytokines, including osteopontin. To determine the involvement of osteopontin in pemphigus vulgaris patients in active stage of the disease, auto-antibodies to desmoglein-1 and desmoglein-3 and plasmatic osteopontin levels were examined by ELISA tests. In this work, significant plasmatic level of osteopontin in PV patients with active stage of disease were found particularly in those patients with... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5386885 Sat, 29 Oct 2011 05:41:32 +0100 5386885 http://www.medworm.com/index.php?rid=5360477&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F415u78162l611p01%2F In conclusion, these preliminary findings further support the possible contribution of the HLA genes in KD pathogenesis. Content Type Journal ArticleCategory Short CommunicationPages 1-9DOI 10.1007/s00403-011-1182-4Authors Barbara Shih, Plastic and Reconstructive Surgery Research, School of Translational Medicine, The Manchester Interdisciplinary Biocentre (MIB), University of Manchester, 131 Princess Street, Manchester, M17ND UKArdeshir Bayat, Plastic and Reconstructive Surgery Research, School of Translational Medicine, The Manchester Interdisciplinary Biocentre (MIB), University of Manchester, 131 Princess Street, Manchester, M17ND UK Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5360477 Fri, 28 Oct 2011 06:05:33 +0100 5360477 http://www.medworm.com/index.php?rid=5360478&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk357341018812812%2F Abstract  Irradiation by long-term ultraviolet (UV) A initiates the induction of photoaging. However, the mechanisms responsible for the structural changes of skin induced by UVA irradiation of the eye are still unknown. Male hairless mice were used in this study. The eye or dorsal skin was locally exposed to UVA after covering the remaining body surface with aluminum foil at a dose of 110 kJ/m2 using a FL20SBLB-A lamp for 60 days. The plasma α-melanocyte stimulating hormone (α-MSH), nitrogen oxides (NO2/NO3), tumor necrosis factor-α (TNF-α), and the prostaglandin E2 (PGE2) content all increased after UVA irradiation. The levels of NO2/NO3, TNF-α, and PGE2 also increased more after UVA skin irradiation than after UVA eye irradiation. However, the level of α-MSH... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5360478 Fri, 28 Oct 2011 06:05:32 +0100 5360478 http://www.medworm.com/index.php?rid=5360479&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F34586686g1016124%2F Abstract  Keloid is a clinically intractable disease that causes disfigurement, itching, and pain due to abnormal proliferation of fibroblasts and production of collagen. Pirfenidone is a novel anti-fibrotic agent that inhibits the progression of fibrosis occurring in the keloid lesions of the lung and kidney. In order to examine whether pirfenidone has a therapeutic effect on keloid lesions, we prepared an in vitro wound contraction model with keloid fibroblasts. The gel contractility of a mixture of keloid fibroblasts and an acid-soluble collagen solution was examined with/without transforming growth factor (TGF)-β1 in the presence or absence of pirfenidone. Real time RT-PCR was performed to detect mRNA expression of TGFB1, CTGF, aSMA, and Col1A1 quantitatively in keloid ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5360479 Fri, 28 Oct 2011 06:05:31 +0100 5360479 http://www.medworm.com/index.php?rid=5335944&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F966722923j22n787%2F Abstract  Effective delivery of therapeutic agents is the most challenging hurdle in the use of RNA interference for research and in the clinic. Here, we assessed whether a short synthetic peptide, ACSSSPSKHCG (TD-1), could be transported through rat footpad (follicle-free) skin and efficiently deliver small interfering RNA (siRNA) to knock down a target gene. Fluorescence microscopy revealed that topical co-administration of FITC-labeled TD-1 and FAM-labeled siRNA distributed uniformly from the epidermis to the subcutaneous tissue of rat footpad skin. Transmission electron microscopy revealed the absence of cell–cell junctions and enlarged spaces between epithelial cells in the TD-1-treated footpad skin. TD-1 delivery of anti-GAPDH siRNA significantly reduced the level o... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5335944 Tue, 18 Oct 2011 15:59:47 +0100 5335944 http://www.medworm.com/index.php?rid=5324091&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu759485203j47n44%2F Abstract  Botulinum toxins are frequently used in esthetics to improve the appearance of facial wrinkles. In this setting, precise localization of the neurotoxin is required to produce the desired clinical effects. Unwanted effects can occur if the neurotoxin diffuses into untargeted muscle. Therefore, a neurotoxin with low and predictable spread would be preferable for esthetic applications. The aim of this study was to investigate the spread of three approved botulinum toxin type A preparations, with and without complexing proteins, by measuring and comparing the size of the anhidrotic halos they produced following injection of equivalent doses in an identical volume into the forehead of patients. The results showed that incobotulinumtoxinA and onabotulinumtoxinA displaye... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5324091 Sat, 15 Oct 2011 05:48:06 +0100 5324091 http://www.medworm.com/index.php?rid=5297066&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv80vt00061m23n82%2F Abstract  Rosacea is a chronic skin disorder, characterized by persistent painful facial flushing and often accompanied by papules and pustules. To investigate the mechanism of facial flushing in rosacea, acetylcholine was administered by iontophoresis to a 10-mm diameter site in the forehead of 31 patients with rosacea and in 29 controls of similar age and sex distribution. During the iontophoresis, current strengths doubled in eight steps from 2.5 to 320 μA. For each step, skin blood flow was monitored during 60 s of iontophoresis and for 2 min afterwards with laser Doppler flow probes at the site of iontophoresis and 5–8 mm away in the region of axon reflex vasodilatation. Vascular responses to acetylcholine were similar in patients and controls, b... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5297066 Tue, 04 Oct 2011 05:52:14 +0100 5297066 http://www.medworm.com/index.php?rid=5297067&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc854640242m24473%2F This study demonstrated that miR-146a may function as a novel negative regulator to modulate myofibroblast transdifferentiation during TGF-β1 induction by targeting SMAD4. Content Type Journal ArticleCategory Original PaperPages 1-8DOI 10.1007/s00403-011-1178-0Authors Zhen Liu, Center of Plastic Surgery and Burn Repair, The 309th Hospital of PLA, No. 17, Heishanhu Street, Beijing, 100091 ChinaCui-Ling Lu, Department of Critical Care Medicine, The 309th Hospital of PLA, Beijing, 100091 ChinaLi-Ping Cui, Center of Plastic Surgery and Burn Repair, The 309th Hospital of PLA, No. 17, Heishanhu Street, Beijing, 100091 ChinaYong-Liang Hu, Center of Plastic Surgery and Burn Repair, The 309th Hospital of PLA, No. 17, Heishanhu Street, Beijing, 100091 ChinaQi Yu, Center of Plastic Surgery a... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5297067 Tue, 04 Oct 2011 05:52:13 +0100 5297067 http://www.medworm.com/index.php?rid=5285848&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8446602v046h3248%2F Abstract  Atopic dermatitis (AD) is a pruritic, chronic inflammatory skin disease that affects 10–20% of children and 1–3% of adults worldwide. Recent studies have indicated that the ability of Th2 cytokines, such as interleukin-4 (IL-4) to regulate skin barrier function may be a predisposing factor for AD development. The present studies examined the ability of increased Th2 activity to affect cutaneous barrier function in vivo and epidermal thickening. Mice that express a constitutively active Signal Transducer and Activator of Transcription 6 (STAT6VT) have increased Th2 cells and a predisposition to allergic inflammation were used in these studies, they demonstrate that topical treatment with the irritant sodium lauryl sulfate (SLS) caused increased transepidermal w... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5285848 Fri, 30 Sep 2011 05:53:09 +0100 5285848 http://www.medworm.com/index.php?rid=5260888&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl80m9020440841q4%2F Abstract  Photoaging is the premature aging of the skin caused by repeated exposure to sunlight and is characterized by a depletion of the dermal extracellular matrix. This depletion is due to the loss of fibroblast cells and their multiple functions. UVA was revealed as a major inducer of photoaging in various clinical studies. As UVA photons have long wavelength spectra, UVA penetrates deeper into the dermis than UVB and UVC, leading to the induction of cell death, the destruction of the dermal extracellular matrix through the induction of matrix metalloproteinase expression, and the repression of collagen expression. However, the exact effects of UVA on the skin remain a matter of debate. Here, we assess cell cycle stage to demonstrate that NF-κB–p53 crosstalk induces... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5260888 Sat, 24 Sep 2011 05:45:29 +0100 5260888 http://www.medworm.com/index.php?rid=5260889&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv4l1540870867524%2F Abstract  Hepatocyte growth factor (HGF) is a multi-effective molecule, playing important roles in organ growth, tumorigenesis and trauma healing. This experiment aims at studying the promoting function of HGF on the proliferation of human eccrine sweat gland epithelial cells (hESGc) and its relative signal channels. After HGF at different concentrations were added into cells, MTT was adopted to detect the cell proliferations, Annexin-V/PI the cell apoptosis, and Westernblot the expressions of p-AKT, AKT, p-ERK, p-GSK3β, p-IKBα, and β-catenin in hESGc. After adding siRNA c-Met to block HGF or LY294002 to inhibit p-AKT, we used MTT to detect the proliferation of hESGc and Westernblot to detect the expression of β-catenin. As a result, 20–40 ng/mL HGF could promote... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5260889 Tue, 20 Sep 2011 05:50:38 +0100 5260889 http://www.medworm.com/index.php?rid=5237893&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm0p4162156114l52%2F This study aimed to compare the prevalence and risk factors of AGA between policemen and the general population. A cross-sectional survey was conducted by recruiting a total of 758 (78%) of 972 policemen and 740 (80%) of 924 participants in a community-based integrated screening served as a comparison group. The Norwood classification system was used to assess the degree of hair loss. Information on age, family history of androgenetic alopecia, and other possible risk factors was collected with questionnaire interviews. The association analysis between policemen and the general population was limited to participants aged 40–59 years. After controlling for other significant factors, policemen aged 40–59 years had an increased risk of developing AGA compared with the gene... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5237893 Sat, 17 Sep 2011 05:47:34 +0100 5237893 http://www.medworm.com/index.php?rid=5237894&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp7615703612uk681%2F Abstract  Even though psoriasis is a common skin disorder, reports of it involving the oral cavity are exceedingly rare, with less than 100 publications in the literature. Biopsy-proven oral psoriasis has been reported in the oral medical literature, but the commonest oral mucosal findings in most studies are associated non-specific features including fissured and geographic tongue. Case series on this entity have not provided any definitive data to support its existence. From the evidence available to date, it is still unclear if oral psoriasis is a distinct entity or if, indeed, it exists. Content Type Journal ArticleCategory Review ArticlePages 1-5DOI 10.1007/s00403-011-1175-3Authors P. D. Yesudian, Betsi Cadwaladr University Health Board, Bodelwyddan, LL18 5UJ UKR.... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5237894 Fri, 16 Sep 2011 16:44:16 +0100 5237894 http://www.medworm.com/index.php?rid=5237895&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F2r53p65h11274gg0%2F Abstract  Xenobiotic transporters of the ATP-binding cassette (ABC) protein superfamily play important roles in maintaining the biochemical barrier of various tissues, but their precise functions in the skin are not yet known. Screening of the expressions of the known xenobiotic transporter genes in two in vitro keratinocyte differentiation models revealed that the ABCC4 and ABCG2 transporters are highly expressed in proliferating keratinocytes, their expressions decreasing along with differentiation. Abrogation of the ABCC4 and ABCG2 protein functions by siRNA-mediated silencing and chemical inhibition did not affect the proliferation of HaCaT cells. In contrast, disruption of the ABCG2 function had no effect on normal human epidermal keratinocyte proliferation, while the ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5237895 Fri, 16 Sep 2011 05:53:12 +0100 5237895 http://www.medworm.com/index.php?rid=5208952&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F45242841u073w644%2F Abstract  Psoriasis and psoriatic arthritis (PsA) are chronic systemic inflammatory disorders with wide spectrums of cutaneous and musculoskeletal presentations. Management of joint disease in this population can be challenging and often requires the expertise of rheumatology in conjunction with dermatology. A multidisciplinary clinic setting may benefit these patients, and in this study we sought to evaluate the experience of such a model. We performed a retrospective chart review of patients evaluated between October 2003 and October 2009 in the Center for Skin and Related Musculoskeletal Diseases (SARM) at Brigham and Women’s Hospital, Boston, MA, USA, where patients are seen by both an attending rheumatologist and dermatologist. Main outcomes included the presence of ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5208952 Fri, 09 Sep 2011 05:58:54 +0100 5208952 http://www.medworm.com/index.php?rid=5196564&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fhn3p564882w787nl%2F Abstract   Leishmania (Viannia) shawi causes cutaneous lesions in humans. Parasite antigens conferring significant protection against American tegumentar leishmaniosis (ATL) might be important for the development of effective vaccine. Therefore, this work evaluates the protective effect of antigenic fractions released by L. shawi. Antigens released by promastigotes to culture medium were concentrated and isolated by SDS-PAGE. The three main fractions LsPass1 (>75 kDa), LsPass2 (75–50 kDa) and LsPass3 (<50 kDa) were electro-eluted according with their molecular mass. Immunized BALB/c mice were challenged with L. shawi promastigotes and the course of infection monitored during 5 weeks. LsPass1-challenged mice showed no protection, however, a strong d... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5196564 Thu, 01 Sep 2011 06:11:32 +0100 5196564 http://www.medworm.com/index.php?rid=5184143&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4065737613lk7114%2F Abstract  Intercellular adhesion molecule-1 (ICAM-1) expression has been detected in melanocytes around active vitiligo patches as well as in surgically transplanted melanocytes. However, it is unclear whether and how skin injury induces the inappropriate expression of ICAM-1 and other proinflammatory genes in melanocytes. We previously reported that human melanocytes expressed TLR3. We hypothesized that the TLR3 expressed in melanocytes may recognize skin injury by binding to the endogenous ligands secreted by the damaged keratinocytes. Here we showed that RNA released from necrotic keratinocytes induced the upregulation of ICAM-1 protein and mRNA, as shown by FACS and real-time RT-PCR. Use of NF-κB inhibitor prevents upregulation of ICAM-1 in melanocytes indicating a dir... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5184143 Tue, 30 Aug 2011 05:43:15 +0100 5184143 http://www.medworm.com/index.php?rid=5161883&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8h1p655x724k3735%2F Abstract  Psoriasis is a common, immune-mediated inflammatory skin disorder. T helper(h)1 and Th17 lymphocytes contribute to the pathogenesis of psoriasis through the release of inflammatory cytokines that promote further recruitment of immune cells, keratinocyte proliferation and sustained inflammation. The innate immune system is the first line of defence against infection and plays a crucial role in the initiation of the adaptive immune response. The presence of innate immune cells and their products in psoriatic skin plaques suggests a role for innate immunity in this disease. In addition, the innate immune system can direct the development of pathogenic Th cells in psoriasis. In this article, we will summarise the role of the innate immune system in psoriasis with part... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5161883 Tue, 23 Aug 2011 15:53:01 +0100 5161883 http://www.medworm.com/index.php?rid=5161884&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F277058611678x468%2F Abstract  The aim of this study was to determine whether or not promoter polymorphisms of the class I major histocompatibility complex (HLA-E, HLA-F, and HLA-G) are associated with susceptibility to vitiligo. To identify a possible association with vitiligo, 241 patients with non-segmental vitiligo (NSV) and 395 healthy controls were recruited in this study. Three promoter single nucleotide polymorphisms (SNPs; rs1264459 of HLA-E, rs9258170 of HLA-F, and rs1736936 of HLA-G) were analyzed using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and direct sequencing. Multiple logistic regression models (co-dominant 1, co-dominant 2, dominant, recessive, and log-additive models) were applied for odds ratios (ORs), 95% confidence intervals ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5161884 Wed, 17 Aug 2011 05:57:47 +0100 5161884 http://www.medworm.com/index.php?rid=5132233&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7704g8173hu853u1%2F Abstract  Recent advances in the field of nanotechnology have allowed the manufacturing of elaborated nanometer-sized particles for various biomedical applications. A broad spectrum of particles, extending from various lipid nanostructures such as liposomes and solid lipid nanoparticles, to metal, nanocrystalline and polymer particles have already been tested as drug delivery systems in different animal models with remarkable results, promising an extensive commercialization in the coming years. Controlled drug release to skin and skin appendages, targeting of hair follicle-specific cell populations, transcutaneous vaccination and transdermal gene therapy are only a few of these new applications. Carrier systems of the new generation take advantage of improved skin penetrat... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5132233 Fri, 12 Aug 2011 06:16:49 +0100 5132233 http://www.medworm.com/index.php?rid=5126432&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fuw262j701x683232%2F This article reviews the exist documents and provides a molecular mechanism for this negative association. Content Type Journal ArticlePages 1-2DOI 10.1007/s00403-011-1165-5Authors Amir Feily, Department of Dermatology, Jundishapur University of Medical Sciences, Ahvaz, IranNader Pazyar, Department of Dermatology, Jundishapur University of Medical Sciences, Ahvaz, Iran Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5126432 Wed, 10 Aug 2011 15:49:34 +0100 5126432 http://www.medworm.com/index.php?rid=5088603&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ftg86x6188un2100h%2F Abstract  Enhancement strategies are necessary to improve the dermal/transdermal bioavailability of drugs applied to the skin due to its amazing barrier, the stratum corneum. Strategies to overcome this barrier, thus improving drug release to the skin include the use of penetration enhancers, specific delivery systems, supersaturated solutions and physical methods (iontophoresis, electroporation and ultrasound). Delivery of active agents to the skin by liposomal carriers has improved topical therapy in the field of dermatology. The interest in these carriers is based on their potential to enclose various types of biological materials and to deliver them to diverse cell types. Particularly, in recent years liposomes have been shown to be a promising drug-delivery system to t... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5088603 Fri, 29 Jul 2011 16:07:27 +0100 5088603 http://www.medworm.com/index.php?rid=5063898&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw26j86x692217n54%2F Abstract  Wound fluid collected from chronic wounds may be used as a simple gauge of the processes taking place in the tissue. There is lack of information on the optimal conditions for wound fluid procurement. We have studied possible diurnal variations and duration of wound fluid accumulation using retentive hydrophobic foam on the levels of prototypic cytokines [interleukin (IL)-1α, IL-1β], a chemokine (IL-8) and proteinases [matrix metalloproteinase (MMP)-9] in 23 chronic venous leg ulcer patients. Bioactivity of 1 and 24 h wound fluids, and serum was also compared. There were no significant temporal changes in the levels of the above-mentioned four proteins, when comparing three consecutive 8-h intervals starting from 0800 that in turn did not differ significant... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5063898 Sat, 23 Jul 2011 15:44:25 +0100 5063898 http://www.medworm.com/index.php?rid=5030500&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7732126775t1m029%2F Abstract  Psoriasis is a chronic inflammatory skin disease that affects approximately 2–4% of the population. We recently described a novel non-coding RNA, psoriasis susceptibility related RNA gene induced by stress (PRINS), that was overexpressed in non-lesional psoriatic epidermis, and its expression was induced by various stress factors such as serum starvation, contact inhibition, ultraviolet (UV)-B irradiation, viral infection and translational inhibition in HaCaT cells. In the present work we set out to compare the stress and microbial agent-induced PRINS expression in normal human keratinocytes (NHKs) and HaCaT cells. Since nuclear factor-κB (NF-κB) is involved in the cellular stress response, we sought to explore whether there is a connection between the NF-κB ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5030500 Wed, 13 Jul 2011 06:18:29 +0100 5030500 http://www.medworm.com/index.php?rid=5030501&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fux72223410340pk4%2F This study investigated the influence of Hp genetic polymorphism on the development of KS in HIV-positive patients. 661 HIV patients were enrolled in the study with a median age of 35 years and a median follow-up time of 57 months. Hp phenotyping was performed using hemoglobin-supplemented starch gel electrophoresis. In case of low Hp concentration high pressure gel permeation chromatography (HPGPC) was used. The Hp 1-1 phenotype was associated with a significant higher risk of KS compared to the combined group of Hp 2-1 and Hp 2-2 patients (p < 0.0005) which remained significant after adjustment for possible confounding variables (age, gender and AIDS status) (p < 0.001). In contrast, the Hp 2-1 phenotype carried the lowest risk. These findings poin... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=5030501 Tue, 12 Jul 2011 06:04:28 +0100 5030501 http://www.medworm.com/index.php?rid=4946643&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy4j65k06308h761g%2F In this study, we investigated the statistics of circulating Th17-related cytokines and adipokines in psoriasis patients. Our study identified the significant elevation of serum IL-6, IL-21, IL-22, and resistin levels in psoriasis patients. Increased serum levels of IL-22 and adiponectin were positively correlated with Psoriasis Area and Severity Index (PASI). In contrast, serum high molecular weight adiponectin levels were decreased in psoriasis and negatively correlated with PASI. Content Type Journal ArticlePages 1-5DOI 10.1007/s00403-011-1159-3Authors Hideki Nakajima, Department of Dermatology, Kochi Medical School, Kochi University, Kohasu, Oko-cho, Nankoku, 783-8505 JapanKimiko Nakajima, Department of Dermatology, Kochi Medical School, Kochi University, Kohasu, Oko-cho, Na... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4946643 Fri, 17 Jun 2011 11:58:02 +0100 4946643 http://www.medworm.com/index.php?rid=4937041&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu7541704078nv627%2F Abstract  Ascorbic acid, a potential antioxidant, is known to inhibit melanogenesis. However, there are conflicting findings that ascorbic acid has very low stability and acts as a pro-oxidant, eventually increasing proliferation and melanin content in melanoma cells. In the present study, we explored the effects of ascorbic acid on the activity and expression of tyrosinase and melanin pigmentation in the presence and absence of α-melanocyte-stimulating hormone (α-MSH) using B16F10 melanoma cells. The mechanism by which ascorbic acid stimulated the expression of tyrosinase was also investigated. No inhibitory effect on melanin content was observed in ascorbic acid-treated cells, regardless of the presence of α-MSH. Ascorbic acid stimulated the activity and expression of ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4937041 Sat, 11 Jun 2011 06:30:36 +0100 4937041 http://www.medworm.com/index.php?rid=4937043&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F54076320188742nr%2F This study investigated the anti-melanogenic effect of aromatic (ar)-turmerone on alpha-melanocyte stimulating hormone (α-MSH) and 3-isobuty-1-methxlzanthine (IBMX)-induced tyrosinase (Tyr), tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) expression in B16F10 melanoma cells. We demonstrated that ar-turmerone inhibits α-MSH and IBMX-induced melanin synthesis and tyrosinase activity. Data also showed that ar-turmerone inhibits the expression of tyrosinase, TRP-1, and TRP-2 in α-MSH- and IBMX-stimulated B16F10 cells. In addition, ar-turmerone exhibits stronger anti-melanogenic effects than curcumin. Furthermore, ar-turmerone strongly inhibited α-MSH- and IBMX-induced microphthalmia-associated transcription factor by suppressing the activity of cyclic a... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4937043 Fri, 10 Jun 2011 06:33:26 +0100 4937043 http://www.medworm.com/index.php?rid=4937042&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm2n5528482448780%2F Abstract  Patients with moderate-to-severe plaque-type psoriasis exhibit increased cardiovascular mortality. Recent publications point towards psoriasis-induced insulin resistance as an important pathomechanism driving cardiovascular comorbidity in these patients. As the hormonal status in general and sex hormone-binding globulin (SHBG) in particular serve as sensitive indicators for insulin resistance, we analysed these parameters in the context of a set of multiple additional clinical and laboratory measurements in a cohort of male patients. Of 33 consecutively enrolled male patients receiving continuous systemic therapy for their moderate-to-severe plaque-type psoriasis, 23 male patients for whom all parameters could be collected over a 24-week treatment period were inclu... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4937042 Fri, 10 Jun 2011 06:33:26 +0100 4937042 http://www.medworm.com/index.php?rid=4923314&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh800428307825m4v%2F Abstract  The transient receptor potential vanilloid 1 (TRPV1) channel can be activated by vanilloids, exposure to ultraviolet (UV) irradiation, heat, or protons, and conditions that occur during tissue injury. In the present study, we investigated whether or not TRPV1-specific blocker, 5′-iodoresiniferatoxin (I-RTX), can reduce UV-induced matrix metalloproteinases (MMPs), pro-inflammatory cytokines, cyclooxygenase (COX)-2, and p53 expression in the skin of hairless mice. Our results showed that I-RTX inhibited UV-induced skin thickening, as measured by a caliper, or in hematoxylin and eosin (H&E)-stained sections. UV-induced mRNA and protein expression of MMP-13, MMP-9, MMP-3, and MMP-2 was significantly reduced by I-RTX. We also observed the inhibitory effects of I-RTX ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4923314 Wed, 08 Jun 2011 15:46:36 +0100 4923314 http://www.medworm.com/index.php?rid=4913773&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh6655944p09188nq%2F Abstract  Optical coherence tomography (OCT), a fairly new non-invasive optical real-time imaging modality, is an emergent in vivo technique, based on the interference (Michelson interferometry) of infrared radiation and living tissues, that allows high-resolution, 2- or 3-dimensional, cross-sectional visualisation of microstructural morphology of tissues. OCT provides depth-resolved images of tissues with resolution up to a few micrometers and depth up to several millimetres depending on tissue type. The investigations using OCT to assess skin structure in clinical settings started in the past decade and consequently proved that this imaging method is useful in visualizing subsurface structures of normal skin, including the epidermis, dermoepidermal junction, dermis, hair ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4913773 Tue, 07 Jun 2011 05:43:39 +0100 4913773 http://www.medworm.com/index.php?rid=4913774&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk4024402n8q5x207%2F Abstract  Compelling evidences support an autoimmune basis of non-segmental vitiligo, and dysregulation of CD4+CD25+ regulatory T cell (Treg) is assumed to contribute to the pathogenesis of vitiligo. Serum levels of transforming growth factor-β (TGF-β), an important immunoregulatory cytokine produced by Treg cells, has been reported significantly decreased in patients with vitiligo. However, relation between the decrease in TGF-β and the dysfunction of Treg cells in pathogenesis of vitiligo was still undemonstrated. To further reveal the role of TGF-β in vitiligo, 46 patients with non-segmental vitiligo and 25 age- and sex-matched healthy control subjects were enrolled in the study. CD4+CD25+ T cells isolated from peripheral venous blood with a CD4+CD25+ regulatory T cell... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4913774 Sun, 05 Jun 2011 05:46:48 +0100 4913774 http://www.medworm.com/index.php?rid=4913775&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fbr93748644628462%2F Abstract  The scarcity of bone marrow mesenchymal stromal cells (BMSCs) prompts the search for alternative sources for cell-based bone defects repair. Human dermal fibroblasts (FBs) have been shown to have a high proliferative potential and the capacity to differentiate into an osteogenic phenotype. The easy and repeated harvest in large quantities makes this cell source a potential candidate for bone tissue engineering. The aim of our study was to compare directly the immune phenotype, proliferative capacity and osteogenic differentiation potential of FBs with that of “gold standard” BMSCs or adipose-derived mesenchymal stromal cells (ADSCs), another alternative osteoprogenitor cell source. Flow cytometry demonstrated that FBs, ADSCs and BMSCs shared common cell surfac... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4913775 Sun, 05 Jun 2011 05:46:47 +0100 4913775 http://www.medworm.com/index.php?rid=4913776&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fjx2q8323q880vu18%2F The objective of this work was to compare tumor and normal skin microcirculation in patients using the orthogonal polarization (OPS) technique. Forty histologically confirmed basal cell carcinomas of 36 patients were evaluated in order to accomplish our objective. Compared to control skin, basal cell carcinomas had significantly greater capillary diameters (10.6 ± 2.6 and 18.5 ± 6.2 μm, respectively), capillary density (25.0 ± 10.4 and 50.3 ± 16.9 cm/cm2, respectively) and red blood cell velocities (0.06 ± 0.03 and 0.11 ± 0.07 mm/s). Our results have demonstrated significant microcirculatory alterations in skin tumors using the OPS technique that allows its transcutaneous evaluation in vivo, in the abse... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4913776 Sun, 05 Jun 2011 05:46:46 +0100 4913776 http://www.medworm.com/index.php?rid=4904264&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq8180434n25742l5%2F Abstract  Chemokines are involved in the pathogenesis of various vascular inflammations. However, information about chemokines in Henoch-Schonlein purpura (HSP) is limited. Herein, we investigated the serum CCL5, CXCL16, and CX3CL1 levels in HSP patients with controls and the ability of sera from HSP patients on chemokine production in human dermal microvascular endothelial cells. Enzyme-linked immunosorbent assay (ELISA) detected serum CCL5, CXCL16, and CX3CL1 levels in patients with HSP. Human dermal microvascular endothelial cell line (HMEC-1) was treated with sera from patients with HSP at different stages, patients with acute spontaneous urticaria, or controls. Serum levels of CCL5, CXCL16, and CX3CL1 were elevated in HSP patients at acute stage, which correlated with ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4904264 Fri, 03 Jun 2011 06:01:15 +0100 4904264 http://www.medworm.com/index.php?rid=4806050&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl7u641rv28715l00%2F Abstract  Apoptosis of single keratinocytes (KC) is a characteristic feature of spongiosis formation, the histopathologic hallmark of acute eczematous dermatitis. In acute eczema, activated dermis-infiltrating T cells secrete several proinflammatory cytokines which might be decisive for KC apoptosis or survival. We analyzed the role of tumor necrosis factor alpha (TNF-α) in the determination of KC fate during spongiosis formation in acute eczematous dermatitis. Supernatants of activated human CD4+ T cells induced apoptosis in primary KC, which could be fully inhibited by individual blockade of interferon-γ (IFN-γ) and CD95 but not by neutralization of TNF-α activity. As compared to CD95-triggering alone, synchronous CD95 and TNF receptor cross-linking in the presence of ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4806050 Sat, 07 May 2011 15:45:06 +0100 4806050 http://www.medworm.com/index.php?rid=4771979&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn7532x3474801j55%2F Abstract  Darier’s disease (DD, OMIM 124200) is an autosomal dominant inherited genodermatosis characterized by warty papules and plaques in seborrheic areas, and loss of adhesion between suprabasal epidermal keratinocytes (acantholysis) and abnormal keratinisation (dyskeratosis). Till date, more than 150 pathogenic mutations in the ATP2A2 (SERCA2) gene, which encodes the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase isoform 2, have been identified as the genetic basis of DD. Our report of eight DD patients from Austria add seven novel variants (L32P, 149-158del10 each in two different non-consanguineous patients, S72Y, F73S, K460X, 2734delC, T982 M) to the repertoire of ATP2A2 mutations in the DD database which is in line with previous reports that most mutations ar... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4771979 Mon, 25 Apr 2011 16:42:01 +0100 4771979 http://www.medworm.com/index.php?rid=4686532&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm73603h326054502%2F Abstract  The human body has its own innate electrical system that regulates the body’s functions via communications among organs through the well-known neural system. While the effect of low-level electrical stimulation on wound repair has been reported, few studies have examined the effect of electric potential on non-wounded, intact skin. A galvanic couple comprised of elemental zinc and copper was used to determine the effects of low-level electrical stimulation on intact skin physiology using a Dermacorder device. Zn–Cu induced the electrical potential recorded on intact skin, enhanced H2O2 production and activated p38 MAPK and Hsp27 in primary keratinocytes. Treatment with Zn–Cu was also found to reduce pro-inflammatory cytokines, such as IL-1α, IL-2, NO and TNF... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4686532 Tue, 05 Apr 2011 10:01:40 +0100 4686532 http://www.medworm.com/index.php?rid=4686534&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn17r771x180n50h6%2F This study aimed to evaluate the diagnostic usefulness of specific IgE antibody titers for the major buckwheat allergens Fag e 1 and Fag e 2. Specific IgE antibodies were determined using the ImmunoCAP method for native Fag e 1 and Fag e 2, recombinant Fag e 1 and Fag e 2, and crude buckwheat extract (f11) in 10 buckwheat allergy patients, 14 atopic dermatitis patients, and 15 healthy subjects. All buckwheat allergy patients showed positive results for native Fag e 1- and Fag e 2-specific IgE tests and for ImmunoCAP (f11). In contrast, the rates of atopic dermatitis patients with positive results for native Fag e 1- and Fag e 2-specific IgE tests were 64.2% (9/14) and 57.1% (8/14), respectively. The sensitivities of the test using recombinant proteins were lower than those of the tes... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4686534 Sun, 03 Apr 2011 05:44:35 +0100 4686534 http://www.medworm.com/index.php?rid=4686533&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7xx71777m0248278%2F In conclusion, the new undecyl-rhamnoside APRC11 is able to down-regulate the expression of molecules implicated in cutaneous inflammation and whose expression is induced by P. acnes, confirming its potential interest in inflammatory acne. Content Type Journal ArticlePages 1-7DOI 10.1007/s00403-011-1147-7Authors O. Isard, INSERM U892, 9 quai Moncousu, 44093 Nantes Cedex 01, FranceM. Lévêque, Pierre Fabre Dermo-Cosmétique, Cell Pharmacology, 2 rue Viguerie, 31025 Toulouse, FranceA. C. Knol, INSERM U892, 9 quai Moncousu, 44093 Nantes Cedex 01, FranceM. F. Ariès, Pierre Fabre Dermo-Cosmétique, Cell Pharmacology, 2 rue Viguerie, 31025 Toulouse, FranceA. Khammari, INSERM U892, 9 quai Moncousu, 44093 Nantes Cedex 01, FranceJ. M. Nguyen, SEB-PIMESP Saint Jacques Hospital CHU de Nante... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4686533 Sun, 03 Apr 2011 05:44:35 +0100 4686533 http://www.medworm.com/index.php?rid=4665052&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk38gxj68181715gl%2F Abstract  Autologous serum skin test (ASST) is mostly used in chronic spontaneous urticaria (CSU) to show autoreactivity. Interleukin-18 (IL-18) has also been shown to be involved in autoimmune conditions. To investigate the role of autoreactivity assessed by ASST in CSU and respiratory diseases and to investigate whether this autoreactive state is related to IL-18 level or other clinical covariates. Fifty-five patients with CSU (mean age: 40.3 ± 12.3 years), 70 patients with persistent asthma (mean age: 43.7 ± 9.6 years), 21 patients with seasonal allergic rhinitis (SAR) (mean age: 35.5 ± 11.8 years) and 20 normal controls (mean age: 37.7 ± 9.8) were included. All subjects underwent a laboratory examination and skin... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4665052 Tue, 29 Mar 2011 17:35:17 +0100 4665052 http://www.medworm.com/index.php?rid=4665051&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fu643824418522554%2F In this study, the modulation of human keratinocyte response to UVB irradiation by HA (970 kDa) was investigated. Immortalized human keratinocytes (HaCaT) were irradiated by a single dose of UVB and immediately treated with HA for 6 and 24 h. The irradiation induced a significant decrease in the gene expression of CD44 and toll-like receptor 2 6 h after irradiation. The expressions of other HA receptors, including toll-like receptor 4 and the receptor for HA-mediated motility, were not detected in either the control or UVB-irradiated or HA-treated HaCaT cells. UVB irradiation induced a significant decrease in the gene expression of HA synthase-2 and hyaluronidase-2 6 h after irradiation. The expressions of HA synthase-3 and hyaluronidase-3 were not significantly m... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4665051 Tue, 29 Mar 2011 17:35:17 +0100 4665051 http://www.medworm.com/index.php?rid=4659446&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F8310715504304h2t%2F Abstract  To elucidate the effects of redox balance regulation on epidermal pigmentation, we used an antioxidant-rich extract of the herb Melia toosendan (dried mature fruits) to assess its effect on endothelin-1 (EDN1)-stimulated pigmentation in human epidermal equivalents and analyzed its biological mechanism of action. Addition of the Melia toosendan extract elicited a marked depigmenting effect on EDN1-stimulated pigmentation after 14 days of treatment, which was accompanied by a significant decrease in eumelanin content. Real-time RT-PCR and Western blotting revealed that the EDN1-stimulated expression of melanocyte-specific proteins (including tyrosinase) was significantly suppressed at the gene and protein levels by the extract. Signaling analysis with specific in... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4659446 Mon, 28 Mar 2011 05:33:05 +0100 4659446 http://www.medworm.com/index.php?rid=4601534&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fyg73r86r16328651%2F Abstract  Keloid, a fibro-proliferative benign tumor of skin, is characterized by an enriched milieu of growth factors and an abundant accumulation of extracellular matrix (ECM). Transforming growth factor (TGF)-β1 is well known as the crucial fibrogenic cytokine promoting ECM production and tissue fibrosis in keloid forming. Epigenetic modifications have been shown to play a role in the pathogenesis of cancer as well as autoimmune and inflammatory disorders. Recent publication reports epigenetic modifications in keloid fibroblasts that include an altered pattern of DNA methylation and histone acetylation. Therefore, the field of epigenetics may provide a new therapeutic idea for keloid treatment strategies. Currently, there is some evidence from experimental studies that ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4601534 Mon, 14 Mar 2011 06:40:20 +0100 4601534 http://www.medworm.com/index.php?rid=4601535&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy5x143515hv64j94%2F Abstract  Atopic eczema is often worsened by stress. While acute stress is associated with increased turnover of serotonin (5-hydroxytryptamine; 5-HT), chronic stress causes a decrease. In chronic stress, there is a decrease of the 5-HT1A receptor (R)- and an increase in the 5-HT2AR-responsiveness to 5-HT. In the present study, the impact of chronic mild stress on the expression of 5-HT1A and 5-HT2A receptors and serotonin transporter protein (SERT) was investigated in eczematous skin and brain of atopic-like NC/Nga mice. Twenty-four NC/Nga mice were subjected to chronic mild stress for 12 weeks, and eczema was induced by applying a mite antigen (Dermatophagoides pteronyssinus) on the ears for the last 4 weeks. The mice were divided into three groups, eight per gr... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4601535 Mon, 14 Mar 2011 06:40:19 +0100 4601535 http://www.medworm.com/index.php?rid=4595152&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fx5k670023rj5215r%2F Abstract  Human papillomavirus (HPV) infection with low-risk or high-risk subtypes is very common. Infection with HPVs is often a major causative factor for the development of cutaneous benign lesions, cervical cancer, and a number of other tumors. The mechanisms of host immunity to prevent and control HPV infection still remain unclear. The importance of ubiquitination (or ubiquitylation) as an intracellular proteasomal-mediated protein degradation pathway, and as an important modulator for the regulation of many fundamental cellular processes has been valued over the last decade. Although the molecular and cellular mechanisms are not completely established, the critical role of ubiquitination in host immune response to HPV infection has become increasingly apparent. This ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4595152 Sat, 12 Mar 2011 07:01:27 +0100 4595152 http://www.medworm.com/index.php?rid=4595153&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm7m02585p87t6531%2F Abstract  Non-melanoma skin cancers (NMSCs) and psoriasis represent common hyperproliferative skin disorders, with approximately one million new NMSC diagnoses each year in the United States alone and a psoriasis prevalence of about 2% worldwide. We recently demonstrated that the glycerol channel, aquaporin-3 (AQP3) and the enzyme phospholipase D2 (PLD2) interact functionally in epidermal keratinocytes of the skin to inhibit their proliferation. However, others have suggested that AQP3 is pro-proliferative in keratinocytes and is upregulated in the NMSC, squamous cell carcinoma (SCC). To evaluate the AQP3/PLD2 signaling module in skin diseases, we determined their levels in SCC, basal cell carcinoma (BCC) and psoriasis as compared to normal epidermis. Skin biopsies with the... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4595153 Sat, 12 Mar 2011 07:01:26 +0100 4595153 http://www.medworm.com/index.php?rid=4550275&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F46142g080706v161%2F In this report, we investigated the melanogenesis inhibitory and antioxidant effects of Bifidobacterium bifidum culture filtrate. The results revealed that B. bifidum culture filtrate effectively suppresses murine tyrosinase activity and decreases the amount of intracellular melanin in a dose-dependent manner. Additionally, the bacterial culture filtrate-scavenged DPPH and ABTS radicals, and shows potent-reducing power in a dose-dependent pattern. Our results expand the application of B. bifidum culture filtrate in the development and research of skin-whitening ingredients. Content Type Journal ArticlePages 1-5DOI 10.1007/s00403-011-1135-yAuthors Huey-Chun Huang, Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, ROCWen-Ying Hu... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4550275 Wed, 02 Mar 2011 06:58:47 +0100 4550275 http://www.medworm.com/index.php?rid=4550276&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb411712841631372%2F Content Type Journal ArticlePages 1-1DOI 10.1007/s00403-011-1139-7Authors Gennady Rosenblat, Polyol Biotech Ltd, Granot Initiative Center, 38100 D.N. Hefer, IsraelShai Meretski, Polyol Biotech Ltd, Granot Initiative Center, 38100 D.N. Hefer, IsraelJoseph Segal, Polyol Biotech Ltd, Granot Initiative Center, 38100 D.N. Hefer, IsraelMark Tarshis, School of Medicine, Hebrew University, 91120 Jerusalem, IsraelAvi Schroeder, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USAAlexandra Zanin-Zhorov, School of Medicine, New York University, New York, NY 10016, USAGilead Lion, Department of Dermatology, Hebrew University-Hadassah Medical Center, 91120 Jerusalem, IsraelArieh Ingber, Department of Dermatology, Hebrew University-Hadassah M... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4550276 Wed, 02 Mar 2011 06:58:44 +0100 4550276 http://www.medworm.com/index.php?rid=4550277&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F387452hh36384511%2F In this study, we investigated the effects of YKS on the development of AD-like lesions in socially isolated NC/Nga mice compared with the effects of fexofenadine and elucidated the mechanism of the ameliorating effect of YKS on the skin lesions. Ten-week-old male NC/Nga mice were divided into three groups (n = 5/group): the conventional control, the YKS-treated, and the fexofenadine-treated groups, and were kept isolated under conventional conditions for 6 weeks. Measurements were made of dermatitis scores and transepidermal water loss (TEWL), scratching and grooming behaviors. Immunohistochemistry and mRNA levels were also evaluated. We performed similar experiments under specific pathogen free (SPF) conditions that served as a SPF control. YKS and fexofenadine inhibi... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4550277 Wed, 02 Mar 2011 06:58:43 +0100 4550277 http://www.medworm.com/index.php?rid=4550278&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa454156322638725%2F Abstract  The use of dermal substitutes is increasingly widespread but the outcomes of substitute-assisted healing remain functionally deficient. Presently, the most successful scaffolds are acellular polymer matrices, prepared through lyophilization and phase separation techniques, designed to mimic the dermal extracellular matrix. The application of scaffolds containing viable cells has proven to be problematic due to short shelf-life, high cost and death of transplanted cells as a result of immune rejection and apoptosis. Recent advances in biomaterial science have made new techniques available capable of increasing scaffold complexity, allowing the creation of 3D microenvironments that actively control cell behaviour. Importantly, it may be possible through these sophis... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4550278 Wed, 02 Mar 2011 06:58:40 +0100 4550278 http://www.medworm.com/index.php?rid=4522846&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb34q812622157m61%2F Abstract  Autoinflammatory syndromes include an expanding list of diseases characterized by unprovoked recurrent attacks of systemic inflammation with lack of autoantibodies or autoreactive T-cells. This group of conditions encompasses monogenic diseases with Mendelian inheritance which are caused by specific mutations of different genes regulating the innate immunity: familial Mediterranean fever, mevalonate kinase deficiency syndrome, tumor necrosis factor receptor-associated periodic syndrome, cryopyrin-associated periodic syndromes, pyogenic disorders and deficiency of interleukin-1 receptor antagonist: all these diseases can present with dermatological manifestations, which often represent the prominent clinical features or, in some cases, the presenting sign. The purp... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4522846 Tue, 22 Feb 2011 06:58:07 +0100 4522846 http://www.medworm.com/index.php?rid=4502947&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl373651012583877%2F Abstract  In vitiligo, the active melanocytes in the epidermis are totally missing, whereas melanoblast cells in the outer root sheath of hair follicles are not affected. In an attempt to find potent repigmenting agents for vitiligo therapy, pod extracts of Cassia occidentalis was found to be effective in inducing differentiation and migration of mouse melanoblast cell line. Methanolic extract redissolved in DMSO at 12.5 μg/ml was found to cause 3.5- to 3.8-fold melanin induction in melb-a melanoblast cells after 4 days in treatment medium. In addition it induced the tyrosinase activity and altered melb-a cell morphology. Transwell migration assay showed the potential of this herbal candidate to induce direct migration of treated cells. To the best of our knowledg... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4502947 Thu, 17 Feb 2011 08:01:32 +0100 4502947 http://www.medworm.com/index.php?rid=4502948&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F41434l67110348t6%2F Abstract  A first generation vaccine (AS100-1) was manufactured with protein from four cultured Leishmania species, which proved to be effective in the treatment of psoriasis. A single blind trial on 3,132 psoriasis patients revealed 508 (16.2%) subjects with psoriatic arthritis (PsA) that received AS100-1 antigens. The study group was distributed according to percent psoriasis area and severity index (PASI) reduction from PASI 10 to PASI 100. All groups decreased in arthritis score (AS), tender joints counts and nail changes after treatment; the highest decreased in the PASI 100 group. Relapses of psoriasis and PsA had PASI and AS lower than initial values before treatment. Clinical remissions were at lower doses and less time, after the second course of treatment. Peripher... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4502948 Thu, 17 Feb 2011 08:01:31 +0100 4502948 http://www.medworm.com/index.php?rid=4496009&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv011v06824786682%2F In this study we report for the first time the deposition of MBL and ficolins in pemphigus lesions. Eight biopsies of skin lesions of pemphigus vulgaris were studied for in situ deposition of IgG and the complement components MBL, Ficolin 1, Ficolin-2, C1q, C3 and membrane attack complex C5b-9. All biopsies presented deposition of IgG and C3 in the intercellular spaces (ICS) of epidermis. MBL deposition was found in the ICS and basal membrane zone (BMZ) of all specimens, whereas C5b-9 showed deposition only in the ICS, with irregular distribution. Ficolin-2 were positive in 50% (4/8) of biopsies showing deposition in the BMZ. On the other hand, ficolin-1 and C1q were negative in all specimens. Our study suggest that MBL and to a lesser extend Ficolin-2 may bind to altered intercellul... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4496009 Wed, 16 Feb 2011 06:59:40 +0100 4496009 http://www.medworm.com/index.php?rid=4442761&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F6511q3t1h476713n%2F Abstract  Novel drug delivery systems, such as solid lipid nanoparticles (SLN), have been proposed to reduce retinoic acid (RA)-induced skin irritation. However, one question still remains: could it be accomplished without reducing efficacy? To evaluate this question the comedolytic effects and epidermal thickening of RA-loaded SLN were compared to the conventional RA formulations (gel or cream), as well as the potential of these formulations to induce skin irritation. The comedolytic effects and epidermal thickening of these formulations, both containing RA at 0.01 or 0.05%, were investigated in a rhino mouse model, while the studies of RA-induced skin irritation were evaluated through rabbit skin irritation tests and in the rhino mouse model. RA-loaded SLN, as compared to... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4442761 Fri, 04 Feb 2011 17:34:55 +0100 4442761 http://www.medworm.com/index.php?rid=4442762&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd925r49t806k22lv%2F Abstract  Ultraviolet radiation is the major environmental insult to the skin and stimulates the synthesis of melanin in melanocytes, which then distribute it to the neighboring keratinocytes where it confers photo-protection. Skin color results from the paracrine interaction between these two cell types. Recent studies suggest that endocannabinoids are potential mediators in the skin. Here, we investigated whether cannabinoid drugs play a role in melanogenesis and if ultraviolet radiation modifies the cutaneous endocannabinoid system. We used human melanotic melanoma cell line (SK-mel-1) in monoculture or co-culture with human keratinocytes (HaCat). Endocannabinoid levels, cannabinoid receptors expression, and melanin content were evaluated under basal conditions and after... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4442762 Fri, 04 Feb 2011 17:34:54 +0100 4442762 http://www.medworm.com/index.php?rid=4442763&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fb616x1747wnh7580%2F In conclusion, NOS3 gene polymorphism would be risk factors for developing Ps. Content Type Journal ArticlePages 1-5DOI 10.1007/s00403-011-1129-9Authors Pablo Coto-Segura, Dermatology II Department, University Hospital Central of Asturias (HUCA), 33007 Oviedo, SpainEliecer Coto, Molecular Genetics Department, University Hospital Central of Asturias (HUCA), Oviedo, SpainAlbert Mas-Vidal, Dermatology II Department, University Hospital Central of Asturias (HUCA), 33007 Oviedo, SpainBlanca Morales, Molecular Genetics Department, University Hospital Central of Asturias (HUCA), Oviedo, SpainVictoria Alvarez, Molecular Genetics Department, University Hospital Central of Asturias (HUCA), Oviedo, SpainMarta Díaz, Molecular Genetics Department, University Hospital Central of Asturias (HUCA),... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4442763 Fri, 04 Feb 2011 03:33:44 +0100 4442763 http://www.medworm.com/index.php?rid=4442764&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fq318716j01326p46%2F Abstract  Topical 5-aminolevulinic acid (ALA) and methyl aminolevulinate (MAL) photodynamic therapy (PDT) of skin lesions is an accepted treatment for skin tumours but success rates need improvement. The effectiveness of PDT is influenced by availability of oxygen. The aim of this study was to demonstrate, in normal skin, whether a decrease in skin oxygen tension reduces the photodynamic reaction (PDR); and whether the addition of topical hydrogen peroxide can reverse the effect. Topical MAL and red light were administered to the inner forearms of 40 healthy volunteers. Skin oxygen availability was lowered during the illumination phase of the PDT, by applying blanching pressure with a plastic slide. Topical hydrogen peroxide was applied under the pressure slide, immediately... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4442764 Thu, 03 Feb 2011 20:51:18 +0100 4442764 http://www.medworm.com/index.php?rid=4442766&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv11601676q320426%2F In this study, microarray analysis was performed on expanded human skin and normal human skin. Significant difference was observed in 77 genes, which suggest a network of several integrated cascades, including cytokines, extracellular, cytoskeletal, transmembrane molecular systems, ion or ion channels, protein kinases and transcriptional systems, is involved in the skin regeneration during expansion. Among these, the significant expression of some regeneration related genes, such as HOXA5, HOXB2 and AP1, was the first report in tissue expansion. Data in this study suggest a list of candidate genes, which may help to elucidate the fundamental mechanism of skin regeneration during tissue expansion and which may have implications for postnatal skin regeneration and therapeutic intervent... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4442766 Tue, 01 Feb 2011 09:25:12 +0100 4442766 http://www.medworm.com/index.php?rid=4442765&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F571247357211x775%2F Abstract  Bowen’s disease is a neoplastic skin disease, known as squamous cell carcinoma in situ. The treatment options for Bowen’s disease are: cryotherapy, curettage, surgery, topical therapy and radiotherapy. In the past recent years, photodynamic therapy was used as a new treatment method. The purpose of this paper is to summarize the results of clinical and research studies with respect to the photodynamic therapy of Bowen’s disease. A search of three databases was conducted using specific keywords and explicit inclusion and exclusion criteria for the study of photosensitizers, light sources and their efficacy in photodynamic therapy of Bowen’s disease. Two photosensitizers have been used mainly for photodynamic therapy of Bowen’s disease therapy: δ-aminolev... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4442765 Tue, 01 Feb 2011 09:25:12 +0100 4442765 http://www.medworm.com/index.php?rid=4442767&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc1345ptp27322r66%2F In this study we investigated the effect of captopril, an ACE-inhibitor, on acetylcholinesterase and acetylcholine secretion in human keratinocytes. We analyzed the level of acetylcholinesterase in HaCat and NHEK cells by RT-PCR and Western blotting analysis. In addition, the effect of captopril on AChE activity was evaluated. We found that captopril induces a strong AChE up-regulation leading to ACh degradation and reduced secretion. Our results suggest that acantholysis induced by ACE-inhibitors might be linked to altered level of Ach. Content Type Journal ArticlePages 1-7DOI 10.1007/s00403-011-1124-1Authors Adone Baroni, Department of Dermatology, Second University of Naples, Naples, ItalyElisabetta Buommino, Department of Experimental Medicine, Section of Microbiology and Cl... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4442767 Tue, 01 Feb 2011 09:25:10 +0100 4442767 http://www.medworm.com/index.php?rid=4429719&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9065v6r63u0k5678%2F Abstract  TNF-related apoptosis-inducing ligand (TRAIL) is recognized as an important regulator of immune responses during infections and various autoimmune-mediated pathologies. Its role in inflammatory dermatoses is largely unknown. We aimed to investigate the expression of TRAIL and its receptors DR4 and DR5 in psoriasis vulgaris. Immunohistochemistry for TRAIL, DR4 and DR5 was performed on samples of lesional (n = 10) and non-lesional (n = 10) skin of patients with plaque psoriasis and skin of healthy volunteers (n = 10). Expression of TRAIL and its receptors was further examined by means of double immunofluorescence staining and co-localization with CD4, CD8, CD11c, CD68, CD16 and CD56 markers. Immunohistochemical staining for TRAIL was sign... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4429719 Fri, 28 Jan 2011 20:31:10 +0100 4429719 http://www.medworm.com/index.php?rid=4429720&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F385n74pw63423l44%2F The objective of this study was to analyze immunohistochemically whether MCTC mast cells as well as protease inhibitors, squamous cell carcinoma antigens (SCCAs), are present in the uterine cervical SCC. In addition, the effect of tryptase and chymase on uterine cervical SCC cell lines was studied in vitro. Here we report that tryptase- and chymase-positive mast cells are present in significant numbers in the peritumoral stroma of SCC lesions. Also, weak SCCA-2 immunoreactivity is observed in the SCC lesions, but only SCCA-1 in uterine cervical specimens with nonspecific inflammation. In cell cultures, especially chymase, but not tryptase, was shown to induce effective detachment of viable, growing and non-apoptotic SiHa SCC cells from substratum. Chymase also detached viable ME-180 ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4429720 Fri, 28 Jan 2011 06:53:12 +0100 4429720 http://www.medworm.com/index.php?rid=4400950&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv6243443415r52j9%2F In conclusion, both CO2 laser ablation with plume suction and APC treatment seem to have a low risk of HPV contamination of the operation room. Content Type Journal ArticlePages 1-4DOI 10.1007/s00403-010-1119-3Authors Gerhard H. Weyandt, Department of Dermatology, Venerology and Allergology, University of Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, GermanyFranz Tollmann, Institute of Virology and Immunobiology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, GermanyPeter Kristen, Department of Obstetrics and Gynecology, University of Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, GermanyBenedikt Weissbrich, Institute of Virology and Immunobiology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany Journal Archives of Dermatological ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4400950 Wed, 19 Jan 2011 16:57:54 +0100 4400950 http://www.medworm.com/index.php?rid=4378643&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm3404212n53vp058%2F Abstract  Side population (SP) cells have been suggested to be multipotent cancer stem cells. To address whether SP cells exist in epidermal squamous cancer cell line A431, A431 cells dyed with Hoechst 33342 were sorted through flow cytometry. The SP cells were then analyzed by colony-forming and cell proliferation assay. Further, tumorigenicity and microarray analysis were used to compare biological difference between SP and non-SP (NSP) cells. Our results showed that SP cells existed in the A431 cell line, showing higher proliferating and colony-forming ability than NSP cells. Tumors generated from SP cells were larger than those from the NSP cells in NOD/SCID mice. The mRNA microarray profiling revealed that five cancer marker gene expressions were up-regulated and one t... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4378643 Mon, 17 Jan 2011 20:24:46 +0100 4378643 http://www.medworm.com/index.php?rid=4378644&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5r04778546426717%2F Abstract  Asiaticoside (ATS) isolated from the leaves of Centella asiatica possesses strong wound-healing properties and reduces scar formation. However, the specific effects of asiaticoside on the formation of keloidal scars remain unknown. In the present study, we evaluated the in vitro effects of asiaticoside on the proliferation, collagen expression, and transforming growth factor (TGF)-β/Smad signaling of keloid-derived fibroblasts. Fibroblasts isolated from keloid tissue and normal skin tissues were treated with asiaticoside at different concentrations. Afterwards, they were subjected to RT-PCR and Western blot analyses. The inhibitory effects of asiaticoside on fibroblast viability were assayed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (M... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4378644 Mon, 17 Jan 2011 20:24:45 +0100 4378644 http://www.medworm.com/index.php?rid=4378645&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fc74210570r263817%2F Abstract  Breast Tumor Kinase (Brk/PTK6) has a relatively limited expression profile in normal tissue. Its expression is restricted to epithelial cells that are differentiating such as those in the epidermis, and Brk expression appears to be absent from proliferating cells in normal tissue. Also, there is now some evidence to suggest that Brk plays a functional role in the differentiation of the keratinocytes in the epidermis. We have, therefore, investigated the role that Brk/PTK6 plays in normal human primary keratinocytes by suppressing protein levels using RNA interference. We show that as primary human keratinocytes are induced to differentiate in vitro, Brk levels decrease. Decreasing Brk protein levels lead to an increase in the number of cells with a permeable plasm... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4378645 Mon, 17 Jan 2011 20:24:44 +0100 4378645 http://www.medworm.com/index.php?rid=4362825&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Flt4xw236x6h73238%2F Abstract  The stratum corneum (SC) was taken from five atopic dogs by tape stripping (12 strips) of non-lesional areas of the abdomen. The free and protein-bound lipids were extracted and analyzed by thin-layer chromatography after fractionation on aminopropyl-bonded silica gel columns. A very frequent feature was the heterogeneity in the lipid content of consecutive layers. This was even more accentuated for the covalently bound lipids, with variations from one layer to another in the concentrations of cholesterol, omega hydroxylated ceramides and omega hydroxylated long-chain fatty acids. Among the free lipids, large amounts of glucosylceramides were present in canine atopic SC although they are nearly absent from the SC of normal dogs. A heterogeneous distribution of lip... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4362825 Sat, 15 Jan 2011 06:55:20 +0100 4362825 http://www.medworm.com/index.php?rid=4356454&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F77u887475k604221%2F We examined circulating Th17 and Treg in the peripheral blood of PPP patients. PBMC were obtained from healthy volunteer controls (n = 26, mean ± SD age 33.11 ± 9.80 years) and PPP patients (n = 24, age 55.00 ± 12.26 years). The proportion of Th17 among the PBMC was 2.52 ± 0.811% (mean ± SD) in healthy controls and 3.23 ± 1.45% in PPP patients. The proportion of Th17 in the PPP patients was significantly higher than that in the healthy controls (p < 0.05, Student’s t test). PPP patients had significantly fewer Treg (5.69 ± 1.86%) than healthy controls (7.10 ± 1.78%). Th17 was inversely correlated with Treg. Content Type Journal ArticlePages 1-4DOI 10... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4356454 Wed, 12 Jan 2011 17:05:41 +0100 4356454 http://www.medworm.com/index.php?rid=4344250&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj6863685425vk7n1%2F Abstract  Recently, we reported that heparanase plays important roles in barrier-disrupted skin, leading to increased interaction of growth factors between epidermis and dermis and facilitating various cutaneous changes, including epidermal hyperplasia and wrinkle formation. However, the role of heparanase in sun-exposed skin remains unknown. Here, we show that heparanase in human keratinocytes is activated by ultraviolet B (UVB) exposure and that heparan sulfate of perlecan is markedly degraded in UVB-irradiated human skin. The degradation of heparan sulfate resulted in a marked reduction of binding activity of the basement membrane for vascular endothelial growth factor, fibroblast growth factor-2 and -7 at the dermal–epidermal junction. Degradation of heparan sulfate w... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4344250 Mon, 10 Jan 2011 16:09:52 +0100 4344250 http://www.medworm.com/index.php?rid=4344251&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa1383579973981w2%2F Abstract  Psoriasis vulgaris is a chronic inflammatory disorder of the skin, in which activation of keratinocytes and crosstalk between keratinocytes and T cells or dendritic cells are considered to be involved in the pathogenesis of psoriasis vulgaris. Cyclosporin (Cy) A, an immunomodulator, has been used for the treatment of psoriasis vulgaris, but the mechanism of its action on keratinocytes has not been well elucidated as its function on T cells is well known. Previous study indicated that the expression of the unfolded protein response (UPR) markers, GRP78/Bip and HRD1 were poorly expressed in psoriasis vulgaris. To investigate if the UPR in keratinocytes is involved in the pathogenesis of psoriasis vulgaris we assessed immunocytochemistry of normal human skin and psor... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4344251 Mon, 10 Jan 2011 16:09:51 +0100 4344251 http://www.medworm.com/index.php?rid=4302390&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fxr36h725133m1638%2F Abstract  Calcium controls an array of key events in keratinocytes and epidermis: localized changes in Ca2+ concentrations and their regulation are therefore especially important to assess when observing epidermal barrier homeostasis and repair, neonatal barrier establishment, in differentiation, signaling, cell adhesion, and in various pathological states. Yet, tissue- and cellular Ca2+ concentrations in physiologic and diseased states are only partially known, and difficult to measure. Prior observations on the Ca2+ distribution in skin were based on Ca2+ precipitation followed by electron microscopy, or proton-induced X-ray emission. Neither cellular and/or subcellular localization could be determined through these approaches. In cells in vitro, fluorescent dyes have been ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4302390 Thu, 30 Dec 2010 18:03:23 +0100 4302390 http://www.medworm.com/index.php?rid=4301027&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd783663410640l05%2F In this study, we examined the effect of fucoidan on IgE production in human peripheral blood mononuclear cells (PBMC) in vitro. PBMC, obtained from healthy donors or patients with atopic dermatitis (AD) with high levels of serum IgE, were cultured with IL-4 and anti-CD40 antibody in the presence or absence of fucoidan. Fucoidan significantly reduced IgE production in PBMC without affecting cell proliferation and IFN-γ production. Fucoidan also inhibited immunoglobulin germline transcripts of B cells in PBMC, and decreased the number of IgE-secreting cells. The inhibitory effects of fucoidan were similarly observed for both PBMC from patients with AD and those with healthy donors. Our findings indicate that fucoidan suppresses IgE induction by inhibiting immunoglobulin class-switchi... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4301027 Wed, 29 Dec 2010 17:09:29 +0100 4301027 http://www.medworm.com/index.php?rid=4301028&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F50567g33795jl852%2F Abstract  Peoniflorin (PF) extracted from the root of Paeonia lactiflora pall displays anti-inflammation and antioxidant properties in several animal models. Chemokines are vital for directing the movement of circulating leukocytes to the sites of inflammation and are involved in the pathogenesis of various inflammatory skin diseases. Herein, we investigated the effects and potential mechanisms of PF on tumor necrosis factor-α (TNF-α) induced chemokine production in human dermal microvascular endothelial cells. Human dermal microvascular endothelial cell line (HMEC-1) was treated by TNF-α with or without PF. PF markedly attenuated TNF-α-induced chemokines (including CCL2, CCL5, CCL20, CXCL8, CXCL16 and CX3CL1) mRNA expression in HMEC-1. PF also reduced the secretion of t... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4301028 Tue, 28 Dec 2010 15:03:02 +0100 4301028 http://www.medworm.com/index.php?rid=4285859&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fg45053u0583213v1%2F Abstract  The use of botanical compounds such as essential oils has recently become the subject of great interest as a natural means of pest control because of their ovicidal, larvicidal, or adulticidal activity against various insect species including head lice. We tested and compared the efficacy of pure oxygenated monoterpenoids that are main ingredients of essential oils of good biological activity. We used pulegone and citral, components of Aloysia citrodora, and geraniol, citronellol, and linalool, components of Geranium sp. oil. We found that citronellol and geraniol showed the highest knockdown and mortality effect (>60%) on adults of both sexes (50:50%) and third-stage nymphs. Pulegone, linalool, and citral showed knockdown percentages between 42 and 55%, and mor... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4285859 Mon, 20 Dec 2010 21:57:59 +0100 4285859 http://www.medworm.com/index.php?rid=4285860&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy826248170721718%2F This study underlines the importance of dendritic cells, Langerhans cells in particular, in non-segmental vitiligo, in its pathogenesis and in its better therapeutical approach. Content Type Journal ArticleDOI 10.1007/s00403-010-1109-5Authors Francesca Prignano, Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, P.za Indipendenza, 11, 50129 Florence, ItalyF. Ricceri, Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, P.za Indipendenza, 11, 50129 Florence, ItalyB. Bianchi, Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, P.za Indipendenza,... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4285860 Mon, 20 Dec 2010 21:57:55 +0100 4285860 http://www.medworm.com/index.php?rid=4272970&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj7p92783u212n503%2F Abstract  Severe psoriasis is associated with significant cardiovascular mortality. We therefore investigated the effects of systemic therapy on the cardiovascular risk of psoriasis patients. Thirteen consecutive patients receiving fumaric acid esters were included and followed for 24 weeks both clinically and by means of laboratory monitoring, 10 completed the study. Eight of ten patients showed a PASI-50 response. Two of three patients with clinical insulin resistance (Homeostasis Model Assessment of insulin resistance >2.5) showed normal insulin responsiveness at the end of the study. Clinical improvement was paralleled by a reduction of high-sensitive CRP serum levels (median −25%). There was a trend toward reduced serum levels for the vascular endothelial gro... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4272970 Fri, 17 Dec 2010 17:38:47 +0100 4272970 http://www.medworm.com/index.php?rid=4267572&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fa28333315117976r%2F Abstract  Patients with a prosthetic limb report negative skin effects, including irritation, rash and chaffing, which can lead to infection, discomfort and reduced wear time to significantly impact normal activities. The aims were to examine the epidermal integrity (transepidermal water loss, TEWL), stratum corneum (SC) hydration [moisture accumulation rate (MAT)], friction and biomechanical properties in active below the knee amputees and to determine the effects of an inert sock liner on skin condition. The liner reduced hydration, TEWL and friction and increased elasticity versus the amputee’s conventional skin care methods. Residual limb TEWL was increased and MAT was reduced versus the contralateral normal skin. In a second study, we hypothesized that complete occlu... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4267572 Wed, 15 Dec 2010 19:11:50 +0100 4267572 http://www.medworm.com/index.php?rid=4267573&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fjq356346wn56h531%2F Abstract  The aim of this study was to screen the antioxidant, anti-hyaluronidase, and anti-elastase activity of the lyophilized juice of Cucumis sativus fruit (CSLJ). The CSLJ was subjected to DPPH and superoxide radical scavenging assay in reference to butylated hydroxytoluene. The hyaluronidase and elastase inhibitory assay was performed in reference to oleanolic acid. Furthermore, the activities have been rationalized with HPLC analysis of the CSLJ with standard reference compound of ascorbic acid. The CSLJ exhibited DPPH-free radical and superoxide radical scavenging activity, IC50 at a concentration of 14.73 ± 1.42 and 35.29 ± 1.30 μg/mL, respectively. The CSLJ also showed strong anti-hyaluronidase (c P < 0.001) and anti-elastas... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4267573 Tue, 14 Dec 2010 06:39:33 +0100 4267573 http://www.medworm.com/index.php?rid=4242591&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F5w3421h23411k741%2F Abstract  The zinc-finger-type transcriptional factor KLF4 is expressed in a variety of tissues including skin. KLF4 can function as either a tumor suppressor or an oncogene, depending on the type of tissue in which it is expressed, by modulating the expression of various factors. To understand the role of KLF4 in human skin cancer and also to evaluate the expression of cyclin D1, p53, and p21Waf1/Cip1 in relation to the expression of KLF4, we evaluated the pattern of KLF4 expression during UVB-induced skin tumor development in SKH-1 hairless mice and in human skin cancer. We also determined whether there are correlations between the expression of KLF4, cyclin D1, p53, and p21 and non-melanoma skin tumors. KLF4 expression was found in the basal layer of non-irradiated contr... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4242591 Mon, 06 Dec 2010 14:43:55 +0100 4242591 http://www.medworm.com/index.php?rid=4242590&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm6m67k87778j75t2%2F Abstract  The migration and proliferation of keratinocytes is critical to wound re-epithelialization and defects in this function are associated with the clinical phenomenon of chronic non-healing wounds. Advanced glycation end products (AGEs) occur through non-enzymatic glycation of long-lived proteins in diabetes and play important roles in diabetic complications. However, specific roles for AGEs in keratinocyte migration and proliferation, and the underlying molecular mechanisms, have not been fully established. The aim of the current study was to elucidate the interaction between AGE-modified bovine serum albumin (AGE-BSA) and keratinocytes. As a result, we found that AGE-BSA had no effect on the viability of keratinocytes for up to 48 h of incubation with 50 �... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4242590 Mon, 06 Dec 2010 14:43:55 +0100 4242590 http://www.medworm.com/index.php?rid=4203907&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv383452085q70p41%2F Content Type Journal ArticleDOI 10.1007/s00403-010-1098-4Authors Faming Pan, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Hefei, Anhui 230032, ChinaDongqing Ye, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Hefei, Anhui 230032, ChinaKechun Zhang, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Hefei, Anhui 230032, ChinaXiangpei Li, Department of Rheumatology, Anhui Provincial Hospital, Hefei, 230000 ChinaJianhua Xu, Department of Rheumatology, First Affiliated Hospital, Anhui Medical University, Hefei, 230032 ChinaHong Chen, Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical Univers... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4203907 Wed, 24 Nov 2010 18:51:31 +0100 4203907 http://www.medworm.com/index.php?rid=4203908&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9783634t12302251%2F Content Type Journal ArticleDOI 10.1007/s00403-010-1097-5Authors Viroj Wiwanitkit, Wiwanitkit House, Bangkhae, Bangkok, 10160 Thailand Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4203908 Tue, 23 Nov 2010 18:26:23 +0100 4203908 http://www.medworm.com/index.php?rid=4187407&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F35377g71g243453x%2F This study clearly indicates the superiority of CU–PC complex and the phyto-vesicles prepared from CU–PC complex over others in providing enhanced anti-aging, antioxidant and anti-wrinkle effect. Content Type Journal ArticleDOI 10.1007/s00403-010-1096-6Authors Nishant Kumar Gupta, Department of Pharmaceutical Sciences, Dr. H. S. Gour Vishwavidyalaya, Sagar (MP), 470003 IndiaV. K. Dixit, Department of Pharmaceutical Sciences, Dr. H. S. Gour Vishwavidyalaya, Sagar (MP), 470003 India Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4187407 Wed, 17 Nov 2010 21:03:42 +0100 4187407 http://www.medworm.com/index.php?rid=4180129&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv14476w003172046%2F Abstract  Mite antigens play important roles in the pathogenesis of atopic dermatitis (AD). We recently developed a novel air cleaner (KC-850U) using charged plasma cluster ions to eliminate a variety of allergens from house environments. The present work demonstrates the ability of KC-850U to decrease the symptoms of AD induced by mite allergens. Pooled sera from the conventional NC/Nga mice, and AD model animals, were incubated with varying concentrations of the control and KC-850U-pretreated allergens extracted from mite. The incubated mixtures were transferred to wells coated with intact allergens and subjected to ELISA to measure the amounts of immunoglobulin E (IgE) bound to the wells. Kinetic analysis revealed that exposure of mite extracts to plasma cluster ions des... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4180129 Tue, 16 Nov 2010 19:22:32 +0100 4180129 http://www.medworm.com/index.php?rid=4176310&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv30203v175t520g2%2F Abstract  The aim of the present work was to clarify the anti-melanogenic mechanism of non-steroidal anti-inflammatory drugs (NSAIDs). Mefenamic acid, diclofenac, and nimesulide were used in this study, and these drugs inhibit melanin synthesis in B16F1 melanoma cells. To elucidate the anti-melanogenic mechanism of NSAIDs, we performed western blotting analysis for melanogenic proteins, such as tyrosinase, TRP-1, and TRP-2. All NSAIDs used in this study inhibited tyrosinase protein level. Semi-quantitative RT-PCR analysis showed that the depigmentation effect of mefenamic acid and nimesulide might be due to the inhibition of tyrosinase gene transcription. These results indicate that NSAIDs inhibit α-MSH-enhanced melanin synthesis, and are candidate anti-melanogenic agents ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4176310 Mon, 15 Nov 2010 18:14:15 +0100 4176310 http://www.medworm.com/index.php?rid=4176311&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp708482173363g31%2F In conclusion, fibroblastic CD10 expression may down-regulate skin inflammation by degrading SP or reducing its level in the dermal microenvironment. Content Type Journal ArticleDOI 10.1007/s00403-010-1093-9Authors Lining Xie, Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashiku, Fukuoka, 812-8582 JapanMasakazu Takahara, Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashiku, Fukuoka, 812-8582 JapanTakeshi Nakahara, Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashiku, Fukuoka, 812-8582 JapanJunna Oba, Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashiku, Fukuoka,... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4176311 Sat, 13 Nov 2010 06:45:21 +0100 4176311 http://www.medworm.com/index.php?rid=4160216&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F68p738821344175p%2F Abstract  Studies regarding cellular interactions between Langerhans cells and other skin cells are somehow hampered by the difficult cultivation of these cells in vitro. Here, we show that the human MUTZ-3 cell line can be differentiated into Langerhans-like cells in the presence of a cytokine cocktail including GM-CSF, TGF-β1 and TNF-α. We used the expression of langerin, CD1a, CCR6 and the intracellular presence of Birbeck granules to identify the differentiated MUTZ-3 cells (MUTZ-3-LCs). The aim of this study was to integrate MUTZ-3-LCs into a three-dimensional full-thickness skin model. On top of fibroblast-containing collagen matrix a mixture of primary human keratinocytes and MUTZ-3-LCs were seeded and cultured for 24 h. Subsequently, the models were lifted up... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4160216 Wed, 10 Nov 2010 17:23:47 +0100 4160216 http://www.medworm.com/index.php?rid=4135601&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fn754171n52129220%2F Abstract  Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that have a pleiotropic impact on the regulation of differentiation, cell growth, and the metabolism of lipids and glucose. PPARδ agonists display a variety of effects on pro- and anti-tumor processes, and seem to have pro-angiogenic activity at very low concentrations. We analyzed the influence of higher concentrations of PPARδ agonists on angiogenesis and its underlying mechanisms. We found that treatment with PPARδ agonists inhibited the formation of capillary-like structures and endothelial cell migration. Since signaling via the vascular endothelial growth factor receptor-2 (VEGFR2) pathway is critical for angiogenic responses during chronic inflammation and tumo... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4135601 Tue, 02 Nov 2010 17:19:55 +0100 4135601 http://www.medworm.com/index.php?rid=4135602&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fr2u322m1404356j3%2F In conclusions, these findings revealed high prevalence of PHEO in NF1 patients and suggest that, in addition to blood pressure, humoral factors (increased sympathetic activity or neurofibromin), influence the pathogenesis of remodeling of cardiovascular system. Content Type Journal ArticleDOI 10.1007/s00403-010-1090-zAuthors Laura Zinnamosca, Department of Clinical Sciences, Secondary Hypertension Unit, University “Sapienza”, Policlinico Umberto I, Viale del Policlinico 155, 00161 Rome, ItalyLuigi Petramala, Department of Clinical Sciences, Secondary Hypertension Unit, University “Sapienza”, Policlinico Umberto I, Viale del Policlinico 155, 00161 Rome, ItalyDario Cotesta, Department of Clinical Sciences, Secondary Hypertension Unit, University “Sapienza”, Policlinico ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4135602 Tue, 02 Nov 2010 15:22:24 +0100 4135602 http://www.medworm.com/index.php?rid=4127259&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9580281786836311%2F In conclusion, F. deltoidea extract has strong anti-melanogenic activity that is exerted by direct inhibition of tyrosinase enzyme activity and by down-regulation of the expression of genes involved in the melanogenesis pathways. Collectively, data shown in this study strongly suggest that F. deltoidea extract has potential to be used as a novel depigmenting agent for cosmetics. Content Type Journal ArticleDOI 10.1007/s00403-010-1089-5Authors Myoung-Jin Oh, Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul, 100-715 KoreaMariani Abdul Hamid, Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul, 100-715 KoreaSulaiman Ngadiran, Chemical Engineering Pilot Plant, Universiti Teknolog... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4127259 Thu, 28 Oct 2010 07:44:57 +0100 4127259 http://www.medworm.com/index.php?rid=4120539&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl898736032v23383%2F Abstract  Exposing skin to ultraviolet (UV) radiation contributes to photoaging and to the development of skin cancer by DNA lesions and triggering inflammatory and other harmful cellular cascades. The present study tested the ability of unique lipid molecules, polyhydroxylated fatty alcohols (PFA), extracted from avocado, to reduce UVB-induced damage and inflammation in skin. Introducing PFA to keratinocytes prior to their exposure to UVB exerted a protective effect, increasing cell viability, decreasing the secretion of IL-6 and PGE2, and enhancing DNA repair. In human skin explants, treating with PFA reduced significantly UV-induced cellular damage. These results support the idea that PFA can play an important role as a photo-protective agent in UV-induced skin damage. ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4120539 Tue, 26 Oct 2010 16:45:58 +0100 4120539 http://www.medworm.com/index.php?rid=4101277&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fw2717637217p265p%2F Abstract  Technological advances during the last years have enhanced the image quality of the microcirculation. Intravital microscopy (IM) has been considered the “gold standard” for many years, but it can be used mostly in anesthetized animals which is a disadvantage. The nailfold videocapillaroscopy, a non-invasive examination that includes a microscope with an epiillumination system, came afterward, but its major disadvantage is the restricted area available for investigation namely the nailfold capillary bed. The orthogonal polarization spectral (OPS) imaging technique, where reflected light allows the visualization of the microcirculation, was the next non-invasive exam, but it still presents some drawbacks such as suboptimal capillary visualization and image blurr... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4101277 Fri, 22 Oct 2010 18:24:46 +0100 4101277 http://www.medworm.com/index.php?rid=4054039&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm32w2w7668240500%2F Abstract  Many patients elect to have repeat treatments with hyaluronic acid dermal fillers to maintain wrinkle correction, but the clinical performance of these products after repeat treatments has not been formally assessed. The primary objective of this study was to evaluate the effectiveness of Juvéderm injectable gel (Juvéderm Ultra, Juvéderm Ultra Plus, and Juvéderm 30) through 1 year after repeat treatment of nasolabial folds (NLFs) that were previously treated with Juvéderm or Zyplast 6–9 months prior to the repeat treatment. Upon completion of the pivotal IDE clinical trial for Juvéderm, five of the original 11 study sites were selected to participate in an extended follow-up evaluation, and a total of 80 subjects were enrolled. For the Juvéderm-... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4054039 Fri, 08 Oct 2010 17:03:06 +0100 4054039 http://www.medworm.com/index.php?rid=4041578&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F3564856175574298%2F Abstract  Aerobic glycolysis increases in tumor cells and pyruvate kinase (PK) is one of the key enzymes involved; PK exists in different isoforms in various tissues. Tumor M2-PK (TM2-PK) is one of these isoforms and its expression has been observed in various tumor cells, including lymphocytes, and in lymphoproliferative disorders. The present study aimed to compare plasma levels of TM2-PK and serum levels of two established markers of various lymphoproliferative disorders—lactate dehydrogenase (LDH) and β-2 microglobulin, and to evaluate the role of TM2-PK in drug monitorization and disease activity in mycosis fungoides (MF) patients. The study included 27 MF patients and 46 healthy controls. Among the MF patients, 18 were stage IA, 6 were stage IB, 1 was stage IIA, an... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4041578 Wed, 06 Oct 2010 16:44:05 +0100 4041578 http://www.medworm.com/index.php?rid=4005321&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F0800724q34pk6373%2F Abstract  Keloid scars are common benign fibroproliferative reticular dermal lesions with unknown etiology and ill-defined management with high rate of recurrence post surgery. The progression of keloids is characterized by increased deposition of extracellular matrix proteins, invasion into the surrounding healthy skin and inflammation. Fibroblasts are considered to be the key cellular mediators of fibrogenesis in keloid scars. Fibroblast activation protein alpha (FAP-α) and dipeptidyl peptidase IV (DPPIV) are proteases located at the plasma membrane promoting cell invasiveness and tumor growth and have been previously associated with keloid scars. Therefore, in this study we analyzed in further detail the expression of FAP-α in keloid fibroblasts compared to control ski... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=4005321 Sat, 25 Sep 2010 16:57:50 +0100 4005321 http://www.medworm.com/index.php?rid=3999653&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F4355002465377858%2F In conclusion, tea tree oil seems to be a more effective anti-eczematic agent than zinc oxide and clobetasone butyrate, while clobetasone butyrate is superior to both ichthammol and zinc oxide in topical treatment of urticarial reactions. Content Type Journal ArticleDOI 10.1007/s00403-010-1083-yAuthors Joanna Wallengren, Department of Dermatology, Skane University Hospital, 221 85 Lund, Sweden Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological Research) Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3999653 Thu, 23 Sep 2010 17:18:23 +0100 3999653 http://www.medworm.com/index.php?rid=3995282&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fm24808p710577123%2F Abstract  Darier disease (DD; OMIM 124200) is a rare, autosomal dominant hereditary skin disorder characterized by abnormal keratinization and acantholysis. The causes of DD are defects in the ATP2A2 gene, which encodes the sarco/endoplasmic reticulum Ca2+ ATPase isoform 2 (SERCA2). The aim of this study was to report a novel splice-site mutation and to examine the relative quantity expression of ATP2A2 gene in a Chinese family with DD. Polymerase chain reaction (PCR) was carried out to amplify the exons and flanking intron boundaries of the ATP2A2 gene followed by direct sequencing. A novel splice-site mutation (IVS20-6T>A) was found in the family, which was confirmed by creating a novel HinfI (NEB Inc) recognition site and RT-PCR. Real-time quantitative PCR showed approx... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3995282 Mon, 20 Sep 2010 19:41:06 +0100 3995282 http://www.medworm.com/index.php?rid=3995283&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd4318386549m5w01%2F Abstract  Patients with moderate to severe psoriasis are undertreated. To solve this persistent problem, the consensus programme was performed to define goals for treatment of plaque psoriasis with systemic therapy and to improve patient care. An expert consensus meeting and a collaborative Delphi procedure were carried out. Nineteen dermatologists from different European countries met for a face-to-face discussion and defined items through a four-round Delphi process. Severity of plaque psoriasis was graded into mild and moderate to severe disease. Mild disease was defined as body surface area (BSA) ≤10 and psoriasis area and severity index (PASI) ≤10 and dermatology life quality index (DLQI) ≤10 and moderate to severe psoriasis as (BSA > 10 or PASI ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3995283 Mon, 20 Sep 2010 19:41:01 +0100 3995283 http://www.medworm.com/index.php?rid=3978223&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff75h5742126h0521%2F Abstract  To find novel depigmenting agents, a new synthetic compound, N-adamantyl-3,4-dihydroxybenzamide (NADB) was produced and the effects on melanogenesis were investigated. Our results showed that NADB reduced melanin synthesis in a dose-dependent manner in melan-a cells. Tyrosinase activity was also reduced by NADB treatment in melan-a cells. However, NADB did not inhibit tyrosinase activity directly in a cell-free system. Treatment of melan-a cells with NADB caused a marked decrease in protein and mRNA levels of tyrosinase along with tyrosinase-related protein 1 and dopachrome tautomerase. To determine whether NADB reduces skin pigmentation, the dorsal skin of brown guinea pigs was shaved and irradiated with UV for 3 weeks using a solar simulator. Then NADB (2 or... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3978223 Thu, 16 Sep 2010 16:46:36 +0100 3978223 http://www.medworm.com/index.php?rid=3978224&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp8u446h6011721g1%2F Abstract  Chronic inflammatory skin disorders have a major impact on the patients’ health related quality of life. Preliminary studies to date have suggested that additional educational and psychological training programmes may be effective in the management of chronic skin diseases, although more rigid methodology is needed. Our purpose was to investigate the effect on quality of life of a novel multidisciplinary educational programme for patients, 18 years or older, with chronic skin diseases. The 12-week intervention encompasses cognitive education on skin and general health issues, and stress-reducing techniques. Quality of life questionnaires were used to assess the participants at baseline and at the end of the program. These comprehend Dermatology Life Quality... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3978224 Wed, 15 Sep 2010 05:47:20 +0100 3978224 http://www.medworm.com/index.php?rid=3948093&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fy504325827411xr5%2F Abstract  The aim of this study is to determine serum CCL23 levels and their clinical associations in patients with systemic sclerosis (SSc). Serum CCL23 levels were examined by ELISA in 66 patients with SSc, 20 patients with systemic lupus erythematosus, 20 patients with dermatomyositis, and 33 healthy individuals. Serum CCL23 levels were elevated in SSc patients (389.1 ± 199.2 pg/mL) compared with healthy individuals (94.1 ± 85.6 pg/mL; P < 0.001) and patients with systemic lupus erythematosus (43.4 ± 39.3 pg/mL; P < 0.001) or dermatomyositis (132.1 ± 104.5 pg/mL; P < 0.001). Among SSc patients, there were no differences in serum CCL23 levels between those with limited cuta... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3948093 Wed, 08 Sep 2010 05:59:16 +0100 3948093 http://www.medworm.com/index.php?rid=3948094&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj07281tp188v8r2r%2F Abstract  Hematoxylin-stainability of keratohyalin granules (KHG) using biochemical and immunohistochemical techniques is due to the presence of a fibrinogen γ-chain protein. A protein with a molecular weight of 100 kDa was stained with anti-Ted-H-1 monoclonal antibody and hematoxylin solution (hematoxylin-stainable protein). Since the amino acid sequence of the hematoxylin-stainable protein was to that of fibrinogen γ-chain protein, a peptide was synthesized and an antibody against the peptide was produced. This antibody reacted with the hematoxylin-stainable protein and fibrinogen γ-chain protein on immunoblot analysis and with KHG on immunohistochemical examination. Furthermore, a commercial anti-fibrinogen γ-chain protein antibody (Ab) also reacted with the hem... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3948094 Wed, 08 Sep 2010 05:16:24 +0100 3948094 http://www.medworm.com/index.php?rid=3912372&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7x15302x786235u3%2F This study compared the sebum check film with a sebumeter. The skin sebum content of the forehead was measured using a sebum check film and a sebumeter. The findings of the sebum content of healthy controls showed that the sebum dot fields on the sebum check film were significantly correlated with the sebum content measured using the sebumeter (r = 0.774, p < 0.001). In addition, the sebum fields on the sebum check film of AD patients (n = 26) were significantly less than those on the sebum check film of the controls (n = 30; p < 0.05). Furthermore, the analysis of the sebum fields on the sebum check film of the AD patients was significantly correlated with their sebum content findings that were obtained using a sebumeter (r ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3912372 Fri, 27 Aug 2010 17:12:43 +0100 3912372 http://www.medworm.com/index.php?rid=3878251&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fp5m1463w76816841%2F Abstract  Keloid is a fibrotic disease characterized by abnormal accumulation of extracellular matrix (ECM) in the dermis. It is a late spreading skin overgrowth and may be considered a plastic surgeon’s nightmare. In nature, curcuminoid is composed of curcumin, demethoxycurcumin (DMC) and bisdemethoxycurcumin (bDMC). Curcuminoids have been found to inhibit fibrosis. However, their role in the synthesis of ECM in the keloid fibroblasts (KFs) has remained unclear. In this series of studies, a total of seven primary KFs cultures were used as the KFs model for investigating the inhibitory effect of curcuminoids on the expression of ECM and TGF-β1. A sensitive and reproducible HPLC method was developed to provide a quantitative analysis on the cellular uptake of curcuminoids... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3878251 Tue, 17 Aug 2010 17:19:24 +0100 3878251 http://www.medworm.com/index.php?rid=3873892&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fd77kk2805717073p%2F This study aimed to establish subscales of the PBI on the basis of independent and consistent treatment need dimensions. The PBI was used in a cross-sectional study involving n = 500 patients with ten distinct skin diseases, and in a longitudinal acne therapy study (n = 925). PBI dimensions were extracted by factor analysis and varimax rotation in both studies independently, using the longitudinal study data for replication. Factor analysis revealed largely similar need dimensions in both studies. The five-dimensional solution found in the cross-sectional study explained 63.0% of the variance. The need dimensions were named as reducing psychological impairments, reducing social impairments, reducing impairments due to therapy, reducing physical impairments, and buil... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3873892 Mon, 16 Aug 2010 18:02:47 +0100 3873892 http://www.medworm.com/index.php?rid=3865979&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ft5823522h56g425w%2F Abstract  Keloid disease (KD) is a quasineoplastic fibroproliferative tumour of unknown origin causing a progressive, recurrent dermal lesion. KD is not homogeneous in nature and shows phenotypic structural differences between its distinct peripheral margins compared to its centre. The keloid margin is often symptomatically more active with increased dermal cellularity, compared to a symptomatically dormant and hypocellular centre of lesion. The aim of this study was to delineate the morphological components of a keloid scar tissue by measuring the differences between various anatomical locations within the keloid tissue, such as the margin and the centre of the lesion compared to its surrounding normal skin using Fourier transform infrared (FT-IR) microspectroscopy. FT-IR ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3865979 Wed, 11 Aug 2010 06:39:51 +0100 3865979 http://www.medworm.com/index.php?rid=3865980&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Ff1837365mp60601q%2F In this study, we investigated whether proteases from P. acnes could activate PAR-2 on keratinocytes and induce pro-inflammatory cytokines, antimicrobial peptides (AMPs), and matrix metalloproteinases (MMPs) via PAR-2 signaling. We examined PAR-2 expression and protease activity in acne lesions using immunofluorescence staining and in situ zymography. The effect of the culture supernatant of P. acnes on Ca2+ signaling in immortalized keratinocytes (HaCaT) was measured using a fluorescence method. HaCaT cells were treated with P. acnes strain ATCC 6919 culture supernatant, with or without pretreatment with serine protease inhibitor or selective PAR-2 antagonist and the gene expression of pro-inflammatory cytokines, AMPs, and MMPs was detected using real-time reverse transcription-polymer... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3865980 Mon, 09 Aug 2010 19:34:13 +0100 3865980 http://www.medworm.com/index.php?rid=3865981&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F74181581066v5004%2F In conclusion, UVB-induced ROS formation, cell fatality, lipid peroxidation, antioxidant depletion and oxidative DNA damage in HDFa cells is inhibited by sesamol, which, probably through its ROS scavenging activity. Content Type Journal ArticleDOI 10.1007/s00403-010-1072-1Authors S. Ramachandran, Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, 608 002 Tamil Nadu IndiaN. Rajendra Prasad, Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, 608 002 Tamil Nadu IndiaS. Karthikeyan, Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, 608 002 Tamil Nadu India Journal Archives of Dermatological ResearchOnline ISSN 1432-069XPrint ISSN 0340-3696 (Source: Archives of Dermatological R... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3865981 Mon, 09 Aug 2010 19:34:12 +0100 3865981 http://www.medworm.com/index.php?rid=3791929&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fe6362gl176w55ku5%2F Abstract  A case of basal cell carcinoma (BCC) with intratumoural bone formation affecting the eyelid of a 77-year-old lady is described. Bone formation in BCCs is an uncommon event. We revised the 23 cases described in the literature: most of them occurred on the face. The pathophysiological mechanism responsible for ossification in cutaneous lesions remains unclear. The bone often develops adjacent to, or within hair follicles. We evaluated the immunohistochemical expression in the present case of Cbfa1/Runx2, an early marker for osteoblastic progenitor cells. A well-developed lamellar bone with adipose bone marrow was observed both in the superficial stroma and intratumourally. Cbfa1/Runx2 was localised in the osteocytes of the heterotopic bone and in the keratin cysts of... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3791929 Sat, 24 Jul 2010 13:27:46 +0100 3791929 http://www.medworm.com/index.php?rid=3773820&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fv84452tp20088050%2F Abstract  Earlier findings indicate the acrosyringium as the target for the inflammation in the chronic and intensely inflammatory skin disease palmoplantar pustulosis (PPP). The sweat gland apparatus seems to be an immune-competent structure that probably contributes to the defence of the skin. Furthermore, the sweat gland and duct may be a hitherto unrecognized neuroendocrine organ because it expresses cholineacetyl-transferase and acetylcholinesterase, nicotinic receptors, beta-adrenergic and angiotensin receptors. The aim of this study was to obtain further information about neuroendocrine properties of the sweat gland apparatus by examining the expression of common neuroendocrine markers synaptophysin and chromogranins A and B in healthy palmar skin and in PPP skin. Sy... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3773820 Fri, 16 Jul 2010 18:13:45 +0100 3773820 http://www.medworm.com/index.php?rid=3773821&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fl4752211320kv502%2F Abstract  Acute urticaria is defined as evanescent wheals with a duration period of up to 6 weeks. Yet within acute urticaria, IgE-mediated urticaria lasts rarely more than 48 h, whereas longer duration periods are frequently unfruitful with respect to diagnostic work-up. We hypothesize the differences in immunologic features in immediate type urticaria versus prolonged acute urticaria within the model of Anisakis simplex (A. simplex) sensitization-associated urticaria. We included 57 patients with gastro-allergic Anisakiasis (GAA) and urticaria duration of less than 48 h and 17 patients with A. simplex sensitization-associated prolonged acute urticaria (PROL), defined as urticaria duration between 3 days and 6 weeks. As control group served 23 patie... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3773821 Wed, 14 Jul 2010 22:57:14 +0100 3773821 http://www.medworm.com/index.php?rid=3755513&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F7k8g461413163615%2F Abstract  Cutaneous T cell-attracting chemokine (CTACK)/CCL27 and macrophage inflammatory protein (MIP)-3α/CCL20 are the major inflammatory chemokines involved in skin inflammation. The present study showed that roxithromycin (RXM) suppressed the TNFα-induced production of CCL27 and CCL20 in HaCaT keratinocytes and normal human keratinocytes (NHKs) in a dose-dependent manner. The production of CCL20 induced by TNFα was suppressed by the addition of inhibitors of nuclear factor kappa B (NFκB). RXM suppressed NFκB activity induced by TNFα. RXM, by regulating CCL27 and CCL20, may contribute to the modulation of inflammation. Content Type Journal ArticleCategory Short CommunicationDOI 10.1007/s00403-010-1068-xAuthors Masaru Karakawa, University of Tokyo Department of... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3755513 Tue, 13 Jul 2010 14:55:14 +0100 3755513 http://www.medworm.com/index.php?rid=3734845&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F9m462k0424448658%2F Abstract  Evidence exists that microorganisms, particularly in the throat and skin, play a role in the pathogenesis of psoriasis. The aim of this study was to investigate whether evidence for the presence of bacteria, including Streptococcus pyogenes, can be demonstrated in the peripheral blood of patients with guttate and/or chronic plaque psoriasis. Peripheral blood samples from 20 patients with psoriasis, seven guttate, six chronic plaque and seven chronic plaque with associated guttate flare and from 16 control subjects were studied for the presence of bacteria by PCR using universal 16S ribosomal DNA primers and specific primers for S. pyogenes. Sequence analysis of amplified 16S rRNA sequences was used to determine taxonomic identity. Ribosomal bacterial DNA was detect... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3734845 Wed, 07 Jul 2010 06:48:33 +0100 3734845 http://www.medworm.com/index.php?rid=3734846&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fk814315135376500%2F Abstract  PADMA 28 is a multi-component herbal mixture formulated according to an ancient Tibetan recipe. PADMA 28 is known to stimulate collagen production and reduced levels of collagen-degrading matrix metalloproteinases (MMPs). The goal of the present study was to determine whether topical treatment of rat skin with PADMA 28 would improve skin structure/function, and whether subsequently induced abrasion wounds would heal more rapidly in skin that had been pretreated with PADMA 28. Hairless rats were exposed to a potent topical corticosteroid (Temovate) in combination with either DMSO alone or with PADMA 28 given topically. At the end of the treatment period, superficial wounds were created in the skin, and time to wound closure was assessed. Collagen production and mat... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3734846 Tue, 06 Jul 2010 17:00:38 +0100 3734846 http://www.medworm.com/index.php?rid=3708105&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fj845082867t84502%2F Abstract  The possible enantioselectivity in the permeation of the chiral anti-inflammatory drug flurbiprofen across hairless rat skin was studied. The transdermal permeability of individual enantiomers from donor solution containing racemic flurbiprofen (0.1%) and pure enantiomers (0.05%) in isopropyl myristate solution was determined using side-by-side diffusion cells. The permeation profiles of enantiomers (R)- and (S)-flurbiprofen from donor solution containing racemic (RS)-flurbiprofen are comparable. When donor solution contained pure enantiomers, marked differences were observed between the permeation rates of (R)- and (S)-flurbiprofen. The steady-state flux and permeability coefficient were significantly higher for (R)-flurbiprofen in comparison with (S)-flurbiprofen ... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3708105 Sat, 26 Jun 2010 16:18:27 +0100 3708105 http://www.medworm.com/index.php?rid=3700438&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F03l6487801lnj77t%2F Abstract  Generalized vitiligo is an autoimmune disorder characterized by patchy loss of pigmentation due to autoimmune destruction of melanocytes in the involved areas. Vitiligo is a polygenic, multifactorial disorder involving multiple genes and unknown environmental triggers. Recently, genetic variation in NALP1 (also called NLRP1), encoding a key regulator of the innate immune response, has been associated with generalized vitiligo in Caucasians of northern European origin. Here, we have investigated whether NALP1 is also associated with generalized vitiligo in Jordanian Arab patients. We genotyped 8 NALP1 single-nucleotide polymorphisms (SNPs) in 26 generalized vitiligo patients and 61 matched controls unaffected by vitiligo or any other autoimmune disorder. We found tha... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3700438 Fri, 25 Jun 2010 16:52:40 +0100 3700438 http://www.medworm.com/index.php?rid=3692522&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fbr3672042mn25740%2F Abstract  The aim of this study was to evaluate pharmacokinetic parameters of fumaric acid esters (FAE) in psoriasis patients for the first time. For this prupose new HPLC assays were developed. Additionally, physicochemical parameters of FAE were determined, allowing a better interpretation of the in vivo data. In vivo, monomethylfumarate (MMF) and monoethylfumarate (MEF) were detected after t lag = 120 min. T max and c max of MMF were 210 min and 11.2 μM, respectively, 210 min and 5.2 μM for MEF. The half-life of MMF was 38.7 min, and 25.4 min of MEF. The AUC0–∞ of MMF was 172 min μg ml−1 and 63.6 min μg ml−1 of MEF. Data display median of three subjects. No plasma levels of dimethylfu... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3692522 Wed, 23 Jun 2010 18:46:06 +0100 3692522 http://www.medworm.com/index.php?rid=3692523&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2Fh17g661516jn1211%2F In this study, we conducted a histological analysis of facial skin to determine whether potential changes in IGF-1 availability occur in the skin with or without conspicuous pores. Immunohistochemical observations showed that expression of insulin-like growth factor binding protein-3 (IGFBP-3) is limited to the suprapapillary epidermis around facial pores and to basal cells of rete pegs without tips in epidermis with conspicuous pores. In contrast, in basal cells of skin without conspicuous pores, IGFBP-3 expression is very low. Ki-67 and IGF-1 receptor-positive cells are abundant in basal cells in the tips of the rete pegs in skin with typical epidermal architecture around facial pores. No obvious differences were observed in the expression of filaggrin, involucrin, K1, K6 or K17 in... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3692523 Mon, 21 Jun 2010 16:01:27 +0100 3692523 http://www.medworm.com/index.php?rid=3688499&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F206l268706426107%2F Abstract  Drug-induced pemphigus has been reported in patients receiving angiotensin-converting enzyme inhibitors. The aim of this work was to study a group of hypertensive patients without skin diseases treated with angiotensin-converting enzyme (ACE) Inhibitors (I), to verify the presence of serum circulating anti-antibodies. The indirect immunofluorescence showed that 33 sera (52.38%) presented autoantibodies directed to an antigen of the cytoplasm of the superficial epidermal keratinocytes. Two of the 33 positive sera had antibodies to Dsg1 and/or 3 in ELISA. Immunoblot analyses were negative. All the 48 control sera were found to have no circulating antibodies using the three assays. Our results would confirm that ACEI drugs may trigger the production of circulating au... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3688499 Mon, 21 Jun 2010 00:44:49 +0100 3688499 http://www.medworm.com/index.php?rid=3688500&cid=s_33464_12_f&fid=33464&url=http%3A%2F%2Fwww.springerlink.com%2Fcontent%2F754u3680gj368006%2F Abstract  A variety of tight junction (TJ) proteins including claudins, occludin, tricellulin, zonula occludens-proteins and junctional adhesion molecules have been identified in complex localization patterns in mammalian epidermis. Their expression and/or localization is frequently altered in skin diseases including skin tumors. However, our understanding of the function(s) of TJ and TJ proteins in the skin is, even though rapidly increasing, still limited. This review summarizes our current knowledge of the involvement of TJ and TJ proteins in mammalian skin in functions ascribed to TJ in simple epithelia, such as barrier function, polarity, gene expression, proliferation, differentiation, and vesicle transport. Content Type Journal ArticleCategory Mini ReviewDOI 10.1... Archives of Dermatological Research journals http://www.medworm.com/rss/comments.php?id=3688500 Sun, 20 Jun 2010 23:12:17 +0100 3688500