MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'BMC Biochemistry' source. Mon, 06 Feb 2012 13:31:36 +0100 http://www.medworm.com/index.php?rid=5656178&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F13%2F3 Conclusions: We present the first protein-level characterization of a group 4 allergen from storage mites. Due to its high production and IgE reactivity, Aca s 4 is potentially relevant to allergic hypersensitivity. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5656178 Tue, 31 Jan 2012 05:00:00 +0100 5656178 http://www.medworm.com/index.php?rid=5635149&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F13%2F2 Conclusions: The results indicate the presence of an intraprotein pore in HAS and support a model in which HA is translocated to the exterior by HAS itself. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5635149 Wed, 25 Jan 2012 05:00:00 +0100 5635149 http://www.medworm.com/index.php?rid=5603655&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F13%2F1 Conclusions: These results provide the first evidence that lipid-bound apolipoproteins on the surface of spherical HDL particles can behave like lipid-free apoA-I to increase ABCA1 protein levels and activity. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5603655 Mon, 16 Jan 2012 05:00:00 +0100 5603655 http://www.medworm.com/index.php?rid=5532952&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F67 Conclusion: The current study provides the basis for elucidating the structure and mechanism of action of this important O-antigen modifying glucosyltransferase. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5532952 Thu, 22 Dec 2011 05:00:00 +0100 5532952 http://www.medworm.com/index.php?rid=5532953&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F66 Conclusions: These results indicate that PEGylated hemoglobins, provided that a suitable starting hemoglobin variant is chosen, can cover a wide range of oxygen-binding properties, potentially meeting the functional requirements of blood substitutes in terms of oxygen affinity, tetramer stability and NO dioxygenase reactivity. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5532953 Tue, 20 Dec 2011 05:00:00 +0100 5532953 http://www.medworm.com/index.php?rid=5512064&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F65 Conclusions: Substrate recognition by NPP7 includes several important contributions, ranging from cation-pi interactions between F275 and the choline headgroup of all substrates, to tail-group binding pockets that accommodate the inherent flexibility of the lipid hydrophobic tails. Two contributions to the unique ability of NPP7 to hydrolyze SM were identified. First, the second hydrophobic tail of SM occupies a second hydrophobic binding pocket. Second, the leucine residue present at position 107 contrasts with a conserved phenylalanine in NPP enzymes that do not utilize SM as a substrate, consistent with the observed reduction in SM hydrolysis by the NPP7-L107F mutant. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5512064 Fri, 16 Dec 2011 05:00:00 +0100 5512064 http://www.medworm.com/index.php?rid=5500907&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F64 Conclusion: The data of our study suggest that the identified and characterized avian FAR isozymes, FAR1 and FAR2, can be involved in wax ester biosynthesis and in other pathways like ether lipid synthesis. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5500907 Mon, 12 Dec 2011 05:00:00 +0100 5500907 http://www.medworm.com/index.php?rid=5492376&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F63 Conclusions: These data suggest that SR is most active as a noncovalent dimer containing one or more free sulfhydryls in the enzyme's active center or a modulatory site. Buffer composition and reduction/oxidation status during preparation can dramatically impact interpretations of SR activity. These findings also highlight the possibility that SR is sensitive to oxidative stress in vivo. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5492376 Thu, 08 Dec 2011 05:00:00 +0100 5492376 http://www.medworm.com/index.php?rid=5482754&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F62 Conclusions: The DNA recognition sequence of the bifunctional prototype TaqII endonuclease-methyltransferase from Thermus aquaticus has been redefined as recognizing only 5' -GACCGA-3' cognate sites. The reaction conditions (pH and salt concentrations) were designed either to minimize (pH = 8.0 and 10 mM ammonium sulphate) or to enhance star activity (pH = 6.0 and no salt). Redefinition of the recognition site and reaction conditions makes this prototype endonuclease a useful tool for DNA manipulation; as yet, this enzyme has no practical applications. The extension of the Fidelity Index will be helpful for DNA manipulation with enzymes only partially cleaving DNA. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5482754 Mon, 05 Dec 2011 05:00:00 +0100 5482754 http://www.medworm.com/index.php?rid=5464915&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F61 Conclusion: Despite the conservation of a Ser protease catalytic triad in their primary sequences, mycobacterial ClpP1 and ClpP2 do not have conventional peptidase activity toward peptide models and display an unusual mechanism of self-assembly. Therefore, the mechanism underlying their peptidase and proteolytic activities might differ from that of other ClpP proteolytic complexes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5464915 Thu, 01 Dec 2011 05:00:00 +0100 5464915 http://www.medworm.com/index.php?rid=5447399&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F60 Conclusions: The data presented here suggest that among proteins of fibrinolytic systems, the fibrinogen is one of the most sensitive proteins to the action of ultrasound. It has been shown in vitro that ultrasound induced fibrinogen aggregates formation, characterized by the loss of clotting ability and a greater rate of plasminolysis than native fibrinogen in different model systems and under different mode of ultrasound treatment. Under ultrasound treatment of plasminogen and/or t-PA in the presence of fibrin(ogen) the stabilizing effect fibrin(ogen) on given proteins was shown. On the other hand, an increase in the rate of fibrin(ogen) lysis was observed due to both the change in the substrate structure and promoting of the protein-protein complexes formation. (Source: BMC Biochemistry... BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5447399 Wed, 23 Nov 2011 05:00:00 +0100 5447399 http://www.medworm.com/index.php?rid=5417160&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F59 Conclusions: Taken together, our studies provide for the first time a collective functional look on a novel, Pasteurellaceae-specific, SBP subfamily of glutathione binding proteins, which we now term GbpA proteins. Our studies strongly implicate GbpA family SBPs in the priming step of ABC-transporter-mediated translocation of useful forms of glutathione across the inner membrane, and rule out a general role for GbpA proteins in heme acquisition. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5417160 Wed, 16 Nov 2011 05:00:00 +0100 5417160 http://www.medworm.com/index.php?rid=5417161&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F58 Conclusions: In addition to determining in vitro inhibition abilities of a panel of mAbs against BoNT/B1-/B5, this work has determined B12.2 and 2B18.2 to be the best mAbs for sample preparation prior to Endopep-MS. These mAb characterizations also have the potential to assist with mechanistic studies of BoNT/B protection and treatment, which is important for studying alternative therapeutics for botulism. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5417161 Tue, 15 Nov 2011 05:00:00 +0100 5417161 http://www.medworm.com/index.php?rid=5374719&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F57 Conclusions: PPP1R6 exerts a powerful glycogenic effect in cultured muscle cells, more than GM and less than PTG. PPP1R6 protein translocates from a Golgi to cytosolic location in response to glucose. The molecular size and subcellular location of myotube glycogen particles is determined by the PPP1R6, PTG and GM scaffolding. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5374719 Fri, 04 Nov 2011 04:00:00 +0100 5374719 http://www.medworm.com/index.php?rid=5374720&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F56 Conclusion: Collectively, these results provide insight into the unique ability of AdsA to produce adenosine through the consecutive hydrolysis of ATP, ADP and AMP, thereby endowing S. aureus with the ability to modulate host immune responses. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5374720 Fri, 28 Oct 2011 04:00:00 +0100 5374720 http://www.medworm.com/index.php?rid=5310473&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F55 Conclusions: The similar organization, chromosomal location and site of expression, suggests that the novel genes are homologous with the genes of WFDC-type protease inhibitors and semen coagulum proteins, despite the lack of similarity in primary structure of their protein products. Their restricted expression to the epididymis suggests that they could be important for male reproduction. The recombinantly produced proteins are presumably bioactive, as demonstrated with similarly made BPTI, but may have a narrower spectrum of inhibition, as indicated by the lacking activity against eight proteases with differing specificity. Another possibility is that they have lost the protease inhibiting properties, which is typical of Kunitz domains, in favor of hitherto unknown functions. (Source: BMC... BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5310473 Tue, 11 Oct 2011 04:00:00 +0100 5310473 http://www.medworm.com/index.php?rid=5310474&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F54 Conclusions: Striatin orchestrates the regulation of Mst3 by PP2A. It binds Mst3 likely as a dimer with CCM3 via residues lying between striatin's calmodulin-binding and WD-domains and recruits the PP2A A/C heterodimer to its coiled-coil/oligomerization domain. Residues outside the previously reported coiled-coil domain of striatin are necessary for its oligomerization. Striatin-associated PP2A is critical for Mst3 dephosphorylation and inactivation. Upon inhibition of PP2A, Mst3 activation appears to involve autophosphorylation of multiple activation loop phosphorylation sites. Mob3 can associate with striatin sequences C-terminal to the Mst3 binding site but also with sequences proximal to striatin-associated PP2A, consistent with a possible role for Mob 3 in the regulation of Mst3 by PP... BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5310474 Mon, 10 Oct 2011 04:00:00 +0100 5310474 http://www.medworm.com/index.php?rid=5256981&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F53 Conclusions: The developed model suggests a hybrid rapid-equilibrium ping-pong random mechanism for the kinetics of OGDHC, consistent with previously reported mechanisms, and accurately describes the experimentally observed regulatory effects of cofactors on the OGDHC activity. This analysis provides a single consistent theoretical explanation for a number of apparently contradictory results on the roles of phosphorylation potential, NAD(H) oxidation-reduction state ratio, as well as the regulatory effects of metal ions on ODGHC function. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5256981 Mon, 26 Sep 2011 04:00:00 +0100 5256981 http://www.medworm.com/index.php?rid=5246004&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F52 Conclusion: Our results indicate that different oligosaccharides are attached to liver and muscle HJV peptides, which may contribute to different functions of HJV in the two tissues. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5246004 Wed, 21 Sep 2011 04:00:00 +0100 5246004 http://www.medworm.com/index.php?rid=5233055&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F51 Conclusions: Collectively, these results suggest that PGC2 is the first exoPG reported from the pathogen FOC, and we have shown that fully functional PGC2 can be produced in the P. pastoris expression system. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5233055 Thu, 15 Sep 2011 04:00:00 +0100 5233055 http://www.medworm.com/index.php?rid=5233056&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F50 Conclusions: Our results suggest that both 53BP1 and SRC3 have a common function that converge at the BRCA1 promoter and possibly other genes important for DNA repair and genomic stability. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5233056 Tue, 13 Sep 2011 04:00:00 +0100 5233056 http://www.medworm.com/index.php?rid=5190956&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F49 Conclusions: Optimization of the growth conditions and gene arrangement resulted in improved responses to nitro-aromatic inducers. The data also suggests the presence of a previously unknown secondary binding site in DntR, analogous to that of BenM. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5190956 Wed, 31 Aug 2011 23:00:00 +0100 5190956 http://www.medworm.com/index.php?rid=5190957&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F48 Conclusions: We conclude that Celluspots peptide arrays are powerful screening tools for studying the specificity of putative reading domains binding to modified histone peptides. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5190957 Tue, 30 Aug 2011 23:00:00 +0100 5190957 http://www.medworm.com/index.php?rid=5155301&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F47 Conclusions: The long, precisely labeled DNA duplexes obtained behave in very much the same manner as natural DNA and are beyond the range of chemical synthesis. Moreover, the conditions of synthesis closely resemble the natural ones, and all the artifacts accompanying the chemical synthesis of DNA are thus eliminated. The approach proposed seems to be completely general and could be used to label DNA at multiple pre-determined sites and with halogen derivatives of any nucleobase. Access to DNAs labeled with proper models of Hal-NBs at specific position is an -labeled DNA is an indispensable condition for the understanding and optimization of DNA photo- and radio-degradation, which are prerequisites for clinical trials of Hal-NBs in anticancer therapy. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5155301 Tue, 23 Aug 2011 23:00:00 +0100 5155301 http://www.medworm.com/index.php?rid=5155303&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F45 Conclusion: In this study we demonstrated that sialidase protein structure contains a surface loop, highly variable both in sequence and size, connecting two conserved beta-sheets and emerging on the opposite site of the catalytic crevice. These data confirm that sialidase family can serve as suitable model for the study of the evolutionary process based on rapid evolving loops, which may had occurred in sialidases. Giving the peculiar organization of the loop region identified in Gg NEU3, this protein can be considered of particular interest in such evolutionary studies and to get deeper insights in sialidase evolution. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5155303 Mon, 22 Aug 2011 23:00:00 +0100 5155303 http://www.medworm.com/index.php?rid=5155302&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F46 The major leucyl aminopeptidase of the protozoan parasite Trypanosoma cruzi is a homohexameric metalloproteinase belonging to the M17 peptidase family, and could have a function in the nutritional supply of essential amino acids (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5155302 Mon, 22 Aug 2011 23:00:00 +0100 5155302 http://www.medworm.com/index.php?rid=5116866&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F44 ${item.shortDescription} (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5116866 Tue, 09 Aug 2011 23:00:00 +0100 5116866 http://www.medworm.com/index.php?rid=5116867&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F43 ${item.shortDescription} (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5116867 Mon, 08 Aug 2011 23:00:00 +0100 5116867 http://www.medworm.com/index.php?rid=5105921&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F42 ${item.shortDescription} (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5105921 Sun, 07 Aug 2011 23:00:00 +0100 5105921 http://www.medworm.com/index.php?rid=5102618&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F41 ${item.shortDescription} (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5102618 Thu, 04 Aug 2011 23:00:00 +0100 5102618 http://www.medworm.com/index.php?rid=5094079&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F40 ${item.shortDescription} (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5094079 Tue, 02 Aug 2011 23:00:00 +0100 5094079 http://www.medworm.com/index.php?rid=5085115&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F39 ${item.shortDescription} (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5085115 Fri, 29 Jul 2011 23:00:00 +0100 5085115 http://www.medworm.com/index.php?rid=5068465&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F38 ${item.shortDescription} (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5068465 Sun, 24 Jul 2011 23:00:00 +0100 5068465 http://www.medworm.com/index.php?rid=5026675&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F36 Conclusions: Kinase and synthetase enzymes utilise C8D-ATP in preference to non-deuterated ATP. The KIE obtained at low ATP concentrations is clearly a primary KIE demonstrating strong evidence that the bond to the isotopically substituted hydrogen is being broken. The effect of the ATP concentration profile on the KIE was used to develop a model whereby the C8H of ATP plays a role in the overall regulation of phosphoryl transfer. This role of the C8-H of ATP in the regulation appears to have been conserved in all kinase and synthetase enzymes as one of the mechanisms associated with binding of ATP. The induction of the C8-H to be labile by active site residues coordinated to the ATP purine ring may play a significant role in explaining the broad range of Km associated with kinase enzymes.... BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5026675 Tue, 12 Jul 2011 23:00:00 +0100 5026675 http://www.medworm.com/index.php?rid=5026674&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F37 After the publication of the work entitled "Dermatan sulfate in tunicate phylogeny: Order-specific sulfation pattern and the effect of [->4IdoA(2-Sulfate)beta-1->3GalNAc(4-Sulfate)beta-1->] motifs in dermatan sulfate on heparin cofactor II activity", by Kozlowski et al., BMC Biochemistry 2011, 12:29, we found that the legends to Figures 2 to 5 contain serious mistakes that compromise the comprehension of the work. This correction article contains the correct text of the legends to Figures 2 to 5. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5026674 Tue, 12 Jul 2011 23:00:00 +0100 5026674 http://www.medworm.com/index.php?rid=5006150&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F35 Conclusion: Our findings unequivocally emphasized that N-terminal extension of MtMetAP1c contributes towards the functionality of the enzyme presumably by regulating active site residues through "action-at-a-distance" mechanism and we for the first time are reporting this unique function of the enzyme. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=5006150 Mon, 04 Jul 2011 23:00:00 +0100 5006150 http://www.medworm.com/index.php?rid=4991377&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F33 Conclusions: Combined with published functional evidence from targeted knock-out mice, these data support a conserved role for Dact proteins in kinase-regulated biochemistry involving Vangl and Dvl. This strongly suggests that a principal role for all Dact family members is in the PCP pathway or a molecularly related signaling cascade in vertebrates. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4991377 Wed, 29 Jun 2011 23:00:00 +0100 4991377 http://www.medworm.com/index.php?rid=4974494&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F32 Conclusions: Collectively, these results suggest that a function of Ixosc Sult 1 and Sult 2 in Ixodid tick salivary glands may include inactivation of the salivation signal via sulfonation of dopamine or octopamine. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4974494 Sun, 26 Jun 2011 23:00:00 +0100 4974494 http://www.medworm.com/index.php?rid=4891400&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F31 Conclusions: We show that OASS from three different pathogenic bacteria bind substrate and product through two different mechanisms. Results indicate that predominantly entropy driven methionine binding is not mediated through classical hydrophobic binding, instead, may involve desolvation of the polar active site. We speculate that OASS in general, may exhibit two different binding mechanisms for recognizing substrates and products. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4891400 Wed, 01 Jun 2011 23:00:00 +0100 4891400 http://www.medworm.com/index.php?rid=4882601&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F30 Conclusion: The Glu residue at position 106 appears not to be the only factor affecting the copper binding, and it may also play a role in maintaining enzyme conformation. The alpha-helix (L351-G378) may not only block access to the type I copper site but also play a role in substrate specificities of bacterial MCOs. The alpha351-380M mutant catalyzing oxidation of the phenolic substrate DMP effectively would be very useful in green chemistry. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4882601 Mon, 30 May 2011 23:00:00 +0100 4882601 http://www.medworm.com/index.php?rid=4882602&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F29 Conclusions: Our results indicate that in ascidian dermatan sulfates the position of sulfation on the GalNAc in the disaccharide [->4IdoA(2S)beta-1->3GalNAcbeta-1->] is directly related to the taxon and that the 6-O sulfation is a novelty apparently restricted to the Phlebobranchia. We also show that the increased content of [->4IdoA(2S)beta-1->3GalNAc(4S)beta-1->] disaccharide units in dermatan sulfates from Stolidobranchia accounts for the increased heparin cofactor II stimulating activity. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4882602 Sat, 28 May 2011 23:00:00 +0100 4882602 http://www.medworm.com/index.php?rid=4870490&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F28 Conclusions: We propose that proliferation of endothelial cells is induced by a VEGF-A165-ATP complex, rather than VEGF-A165 alone. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4870490 Thu, 26 May 2011 23:00:00 +0100 4870490 http://www.medworm.com/index.php?rid=4870492&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F26 Conclusion: Fibin is functionally relevant during embryogenesis and adult life. Its expression is regulated by a number of cellular signalling pathways and the protein is routed via the secretory pathway. However, proper secretion presumably requires an unknown covalently-linked or associated co-factor (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4870492 Wed, 25 May 2011 23:00:00 +0100 4870492 http://www.medworm.com/index.php?rid=4870491&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F27 Conclusions: A cleaved signal peptide site in LRRC32 is necessary for surface localization of native LRRC32 following activation of naturally-occurring freshly-isolated regulatory T cells. LRRC32 expression appears to alter the surface expression of activation markers of T cells such as CD62L. LRRC32 surface expression may be useful as a marker that selects for more potent Treg populations. In summary, understanding the processing and expression of LRRC32 may provide insight into the mechanism of action of Tregs and the refinement of immunotherapeutic strategies aimed at targeting these cells. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4870491 Wed, 25 May 2011 23:00:00 +0100 4870491 http://www.medworm.com/index.php?rid=4852167&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F24 Conclusions: Based upon these observations, we propose oxidative assembly of multi-subunit adiponectin complexes in a defined and stable redox environment is favored under oxidizing conditions coupled with high rates of disulfide rearrangement. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4852167 Sun, 22 May 2011 23:00:00 +0100 4852167 http://www.medworm.com/index.php?rid=4852166&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F25 Conclusions: The ratio of insulin-stimulated glucose versus AA clearance was decreased 5.4-fold in diabetic pigs, which was caused by a 3.6-fold decrease in glucose clearance and a 2.0-fold increase in non-essential AA clearance. In parallel with the Randle concept (glucose - fatty acid cycle), the present data suggest the existence of a glucose and non-essential AA substrate interaction in diabetic pigs whereby reduced insulin-stimulated glucose clearance seems to be partly compensated by an increase in non-essential AA clearance whereas essential AA are preferentially spared from an increase in clearance. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4852166 Sun, 22 May 2011 23:00:00 +0100 4852166 http://www.medworm.com/index.php?rid=4847148&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F23 Conclusions: We show how the CND alone, and in combination with other regions of non-core RAG1, functions in nuclear localization, zinc coordination, and interactions with nucleic acid. Together, these results demonstrate the multiple roles that the non-core region can play in the function of the full length protein. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4847148 Thu, 19 May 2011 23:00:00 +0100 4847148 http://www.medworm.com/index.php?rid=4839425&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F21 Conclusions: The results suggest that the overall structure of the Pi-transporting unit of the PiT family proteins has remained unchanged during evolution. Moreover, in combination, our studies of the gene structure of the human PiT1 and PiT2 genes (SLC20A1 and SLC20A2, respectively) and alignment of protein sequences of PiT family members from all kingdoms, along with the studies of the dual functions of the human PiT paralogs show that these proteins are excellent as models for studying the evolution of a protein's structure-function relationship. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4839425 Mon, 16 May 2011 23:00:00 +0100 4839425 http://www.medworm.com/index.php?rid=4839424&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F22 Background: Two isoforms of the enzyme adenosine kinase (AdK), which differ at their N-terminal ends, are found in mammalian cells. However, there is no information available regarding the unique functional aspects or regulation of these isoforms. Results: We show that the two AdK isoforms differ only in their first exons and the promoter regions; hence they arise via differential splicing of their first exons with the other exons common to both isoforms. The expression of these isoforms also varied greatly in different rat tissues and cell lines with some tissues expressing both isoforms and others expressing only one of the isoforms. To gain insights into cellular functions of these isoforms, mutants resistant to toxic adenosine analogs formycin A and tubercidin were selected from Chines... BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4839424 Mon, 16 May 2011 23:00:00 +0100 4839424 http://www.medworm.com/index.php?rid=4819450&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F20 Conclusion: In this work, we explore how single amino acid propensities for these nucleotides play a role in the affinity and specificity of this set of nucleotides. This is expected to be helpful in identifying novel binding sites for adenine and guanine phosphates, especially when a known binding motif is not detectable. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4819450 Thu, 12 May 2011 23:00:00 +0100 4819450 http://www.medworm.com/index.php?rid=4794134&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F19 Conclusion: Our data indicate that insulin-stimulated tyrosine phosphorylation of Munc18c impairs the ability of Munc18c to bind its cognate SNARE proteins, and may therefore represent a regulatory step in GLUT4 traffic. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4794134 Thu, 05 May 2011 23:00:00 +0100 4794134 http://www.medworm.com/index.php?rid=4741627&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F16 Conclusions: Since Dnnmt3a has been associated with development, cancer and brain function, these data suggest a biochemical mechanism for the beneficial health effect of black tea and coffee and a possible molecular mechanism for the improvement of brain performance and mental health by dietary polyphenols. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4741627 Wed, 20 Apr 2011 23:00:00 +0100 4741627 http://www.medworm.com/index.php?rid=4718879&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F15 Conclusions: These obtained results clearly indicate that bergapten and citropten are strong inhibitors of IL-8 expression and could be proposed for further studies to verify possible anti-inflammatory properties to reduce lung inflammation in CF patients. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4718879 Thu, 14 Apr 2011 23:00:00 +0100 4718879 http://www.medworm.com/index.php?rid=4651842&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F14 Conclusions: Taken together, our data suggest an important role of ATP in FGF2-stabilization beside the well known-role of heparin and heparan sulphate. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4651842 Mon, 28 Mar 2011 23:00:00 +0100 4651842 http://www.medworm.com/index.php?rid=4635801&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F13 Conclusions: CD98 and intercellular adhesion molecule-1 are major carriers of CD15/Lewis-x on Reed-Sternberg cells. Binding of DC-SIGN and other glycan-specific receptors to the Lewis-x epitopes on CD98 and intercellular adhesion molecule-1 may facilitate interaction of the lymphoma cells with lymphocytes and myeloid cells in lymph nodes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4635801 Thu, 24 Mar 2011 00:00:00 +0100 4635801 http://www.medworm.com/index.php?rid=4549212&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F12 Conclusions: Our data therefore suggest that at least in the case of sterol carrier protein 2 the contribution of the second binding site is not essential for peroxisomal import. At this stage, however, we cannot rule out that other cargo proteins may require this ancillary binding site. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4549212 Fri, 04 Mar 2011 00:00:00 +0100 4549212 http://www.medworm.com/index.php?rid=4511548&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F11 Conclusion: These results show that residues at the +2 subsite are interesting targets for mutagenesis and mutations at these positions can directly or indirectly affect both KM and turnover. An affinity change, leading to a decreased KM, can be explained by an altered position of N291, while the changes in turnover are more difficult to explain and may be the result of smaller conformational changes in the active site. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4511548 Wed, 23 Feb 2011 00:00:00 +0100 4511548 http://www.medworm.com/index.php?rid=4488652&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F9 Conclusions; MCRA protein in B. breve can be classified as a FAD-containing double bond hydratase, within the carbon-oxygen lyase family, which may be catalysing the first step in conjugated linoleic acid (CLA) production, and this protein has an additional function in bacterial stress protection. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4488652 Thu, 17 Feb 2011 00:00:00 +0100 4488652 http://www.medworm.com/index.php?rid=4449627&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F7 Conclusion: We suggest that differential effects of PKAI and PKAII in inducing Cre-luciferace activity depend on R and not C subunit identity. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4449627 Tue, 08 Feb 2011 00:00:00 +0100 4449627 http://www.medworm.com/index.php?rid=4432586&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F6 Conclusions: The distribution of porcine Dsp posttranslational modifications indicate that porcine Dsp has an N-terminal domain with at least six N-glycosylations and a C-terminal domain with two GAG attachments and at least two O-glycosylations. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4432586 Thu, 03 Feb 2011 00:00:00 +0100 4432586 http://www.medworm.com/index.php?rid=4423505&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F4 Conclusions: SUMO-1 increases the enzymatic turnover of TDG by overcoming the product-inhibition of TDG on apurinic sites. The mechanism involves a competitive DNA binding activity of SUMO-1 towards the regulatory domain of TDG. This mechanism might be a general feature of SUMO-1 regulation of other DNA-bound factors such as transcription regulatory proteins. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4423505 Tue, 01 Feb 2011 00:00:00 +0100 4423505 http://www.medworm.com/index.php?rid=4404841&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F3 Conclusions: Our results show that the residues of HscB most important for strong interaction with IscU include three hydrophobic residues (L92, L96, and F153); in addition, we identified a number of other residues whose side chains contribute to a lesser extent to the interaction. Our results suggest that the triple alanine substitution at HscB positions 92, 96, and 153 will destabilize the HscB-IscU complex by 5.7 kcal/mol, equivalent to an approximately 15000-fold reduction in the affinity of HscB for IscU. We propose that this triple mutant could provide a more definitive test of the functional importance of the HscB-IscU interaction in vivo than those used previously that yielded inconclusive results. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4404841 Wed, 26 Jan 2011 00:00:00 +0100 4404841 http://www.medworm.com/index.php?rid=4308861&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F12%2F1 Conclusions: These results indicate that the B-box 2, coiled-coil and linker 2 regions of TRIM5alpha form a core dimerization motif that exhibits a high level of alpha-helical content. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4308861 Tue, 04 Jan 2011 00:00:00 +0100 4308861 http://www.medworm.com/index.php?rid=4301928&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F50 Conclusions: The small 38-amino acid synthetic SBP offers the potential to successfully perform all four critical analytical procedures as a single step and should have a general utility for the study of many proteins and protein complexes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4301928 Fri, 31 Dec 2010 00:00:00 +0100 4301928 http://www.medworm.com/index.php?rid=4292903&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F49 Conclusions: In yeast GAPDH activity is largely unresponsive to low to moderate H2O2 doses. This points to a scenario where (a) cellular redoxins efficiently cope with levels of GAPDH oxidation induced by a vast range of sub-lethal H2O2 concentrations, (b) inactivation of GAPDH cannot be considered a sensitive biomarker of H2O2-induced oxidation in vivo. Since GAPDH inactivation only occurs at cell death-inducing high H2O2 doses, GAPDH-dependent rerouting of carbohydrate flux is probably important merely in pathophysiological situations. This work highlights the importance of studying H2O2-induced oxidative stress using concentrations closer to the physiological for determining the importance of protein oxidation phenomena in the regulation of cellular metabolism. (Source: BMC Biochemistry... BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4292903 Tue, 28 Dec 2010 00:00:00 +0100 4292903 http://www.medworm.com/index.php?rid=4218746&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F47 Conclusions: Based on the obtained results, we suggest that pepG1 can be added to the MEROPS family G1 as the first characterized bacterial member. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4218746 Wed, 01 Dec 2010 00:00:00 +0100 4218746 http://www.medworm.com/index.php?rid=4189163&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F46 Conclusions: We have confirmed the activity of CpFAS1-R by directly assaying its substrate preference and kinetic parameters, which is for the first time for a Type I FAS, PKS or non-ribosomal peptide synthase (NRPS) reductase domain. The restricted substrate preference towards very long chain fatty acyl thioesters may be an important feature for this megasynthase to avoid the release of product(s) with undesired lengths. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4189163 Mon, 22 Nov 2010 00:00:00 +0100 4189163 http://www.medworm.com/index.php?rid=4174964&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F45 Conclusions: The specificities of the enzyme indicate that this novel Tetrahymena exonuclease is distinct from Exo1 and has properties required for 3'-overhang formation at telomeres. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4174964 Tue, 16 Nov 2010 00:00:00 +0100 4174964 http://www.medworm.com/index.php?rid=4149643&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F44 Conclusions: Intact insulin only weakly interacts with the hsp70 chaperone DnaK whereas monomeric proinsulin and peptides from 3 distinct proinsulin regions show substantial chaperone binding. Strongest binding was seen for the B-chain peptide B 11-23. Interestingly, peptide B11-23 represents a dominant autoantigen in type 1 diabetes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4149643 Mon, 08 Nov 2010 00:00:00 +0100 4149643 http://www.medworm.com/index.php?rid=4132298&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F43 Conclusions: Our observations suggest soluble forms of PlnDI are biologically active. Moreover, PlnDI heparan sulfate chains alone or together with VEGF165 can enhance VEGFR-2 signaling and angiogenic events, in vitro. We propose PlnDI liberated during basement membrane or extracellular matrix turnover may have similar activities, in vivo. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4132298 Wed, 03 Nov 2010 00:00:00 +0100 4132298 http://www.medworm.com/index.php?rid=4116790&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F42 Conclusions: Collectively, the data suggest that the N-terminus of Ack1 promotes membrane localization and dimerization to allow for autophosphorylation. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4116790 Tue, 26 Oct 2010 23:00:00 +0100 4116790 http://www.medworm.com/index.php?rid=4011307&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F40 Conclusions: Two novel GlcNAcases were identified as exolytic enzymes that degraded chitin oligosaccharides, releasing GlcNAc as the end product. In living cells, these intracellular enzymes may work after endolytic chitinases to complete chitin degradation. The availability of the two GlcNAcases, together with the previously-reported chitinase A from the same organism, suggests that a systematic development of the chitin-degrading enzymes may provide a valuable tool in commercial chitin bioconversion. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=4011307 Tue, 28 Sep 2010 23:00:00 +0100 4011307 http://www.medworm.com/index.php?rid=3997813&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F38 Conclusions: Taken together, males had higher ratios of insulin to glucagon levels, higher levels of glycogen, lower degree of AMPK phosphorylation, higher expression of gluconeogenic genes and higher hepatic glucose output. Possibly these sex-differences reflect a higher ability for the healthy male rat liver to respond to increased energy demands. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3997813 Wed, 22 Sep 2010 23:00:00 +0100 3997813 http://www.medworm.com/index.php?rid=3992427&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F37 Conclusions: The minimal functional unit of Mcm4/6/7 is a hexamer. One of the roles of ATP binding by Mcm4/6/7 may be to stabilize formation of hexamers. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3992427 Tue, 21 Sep 2010 23:00:00 +0100 3992427 http://www.medworm.com/index.php?rid=3992428&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F36 Conclusions: Studies have shown that decreased AMPK activity is associated with obesity and Type II Diabetes, while AMPK activity is increased with metabolically favorable conditions and cholesterol lowering drugs. These results suggest a role for beta-arrestin in the inhibition of AMPK signaling, raising the possibility that beta-arrestin-dependent PAR2 signaling may act as a molecular switch turning a positive signal to AMPK into an inhibitory one. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3992428 Mon, 20 Sep 2010 23:00:00 +0100 3992428 http://www.medworm.com/index.php?rid=3986282&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F35 Conclusions: The Rv1694 has an unusual dual activity. It appears to contain two diverse functions such as haemolytic activity and ribosomal RNA methylation activity. It is possible that the haemolytic activity might be relevant to intra-cellular compartments such as phagosomes rather than cell lysis of erythrocytes and the self-assembly trait may have a potential role after successful entry into macrophages by Mycobacterium tuberculosis. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3986282 Sun, 19 Sep 2010 23:00:00 +0100 3986282 http://www.medworm.com/index.php?rid=3898765&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F32 Conclusions: Homologous production and evolutionary studies of the first LMCO from the biomass-degrading fungus T. reesei gives new insights into the physicochemical parameters and biodiversity in this family. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3898765 Mon, 23 Aug 2010 23:00:00 +0100 3898765 http://www.medworm.com/index.php?rid=3888491&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F31 Conclusions: Eighteen potentially secreted sulfhydryl oxidases were detected in the publicly available fungal genomes analysed and a novel proline-tryptophan dipeptide in the characteristic motif CXXC, where X is any amino acid, were found. A representative protein, AoSOX1 from A. oryzae, was produced in T. reesei in an active form and had the characteristics of sulfhydryl oxidases. Further testing of the activity on thiol groups within larger peptides and on protein level will be needed to assess the application potential of this enzyme. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3888491 Thu, 19 Aug 2010 23:00:00 +0100 3888491 http://www.medworm.com/index.php?rid=3822174&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F29 Conclusion: Under mild stress conditions the basal activity of Gcn2p is sufficient to allow for proper adaptation to amino acid depletion. This study highlights the importance of the GCN2 eIF2 kinase pathway for maintaining metabolic homeostasis, contributing to appropriate tRNA charging and growth adaptation in response to culture conditions deficient for the central amino acids, trypotphan and arginine. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3822174 Tue, 03 Aug 2010 23:00:00 +0100 3822174 http://www.medworm.com/index.php?rid=3790078&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F28 Conclusions: The Drosophila beta-CA represents a highly active mitochondrial enzyme that is a potential model enzyme for anti-parasitic drug development. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3790078 Sun, 25 Jul 2010 23:00:00 +0100 3790078 http://www.medworm.com/index.php?rid=3740073&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F26 Conclusions: The findings that both yeast and Drosophila SIN3 affect mitochondrial activity suggest an evolutionarily conserved role for SIN3 in the control of cellular energy production. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3740073 Thu, 08 Jul 2010 23:00:00 +0100 3740073 http://www.medworm.com/index.php?rid=3717809&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F25 Conclusions: The relative quantification of Cys-SOH within specific proteins in a complex biological setting can be accomplished, but several analytical precautions related to trapping, detecting, and quantifying Cys-SOH must be taken into account prior to pursuing its study in such matrices. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3717809 Wed, 30 Jun 2010 23:00:00 +0100 3717809 http://www.medworm.com/index.php?rid=3709917&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F24 Conclusions: SHP is not as rare as generally believed and has a role to play in the photosynthetic bacteria. Furthermore, the two companion cytochromes along with SHP are likely to function as an electron transfer pathway that results in the reduction of SHP by quinol and formation of the oxygen complex, which may function as an oxygenase. The three distinct sensors suggest at least as many separate functional roles for SHP. Two of the sensors are not well characterized, but the third is homologous to the QseC quorum sensor, which is present in a number of pathogens and typically appears to regulate genes involved in virulence. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3709917 Mon, 28 Jun 2010 23:00:00 +0100 3709917 http://www.medworm.com/index.php?rid=3665395&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F23 Conclusions: Our results for the first time link the stimulatory effect of flavonoids on CYP expression to their inhibitory effect on Cdks, through a PXR-mediated mechanism. These results may have important implications on the pharmacokinetics of drugs co-administered with herbal remedy and herbal-drug interactions. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3665395 Tue, 15 Jun 2010 23:00:00 +0100 3665395 http://www.medworm.com/index.php?rid=3606866&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F21 Conclusions: The unknown role of maltokinases in mycobacterial metabolism and the lack of biochemical data led us to express the mak gene from M. bovis BCG for biochemical characterization. This is the first mycobacterial Mak to be characterized and its properties represent essential knowledge towards deeper understanding of mycobacterial physiology. Since Mak may be a potential drug target in M. tuberculosis, its high-level production and purification in bioactive form provide important tools for further functional and structural studies. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3606866 Wed, 26 May 2010 23:00:00 +0100 3606866 http://www.medworm.com/index.php?rid=3606865&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F22 Conclusions. Given the high structural homology between the archaeal and eukaryotic proteins, the effect of archaeal Nip7 and SBDS on the exosome provides a model for an evolutionarily conserved exosome control mechanism. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3606865 Wed, 26 May 2010 23:00:00 +0100 3606865 http://www.medworm.com/index.php?rid=3581008&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F20 Conclusion: We present a non-radioactive and highly sensitive method for the measurement of endogenous activities of DYRKs in biological samples. Xenopus laevis oocytes contain an active DYRK1-related protein kinase more similar to mammalian DYRK1B than DYRK1A. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3581008 Wed, 19 May 2010 23:00:00 +0100 3581008 http://www.medworm.com/index.php?rid=3576723&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F19 Conclusion: The characteristics reported here could be used to develop specific assay methods for each of the four murine KATs. These specific assays could be used to identify which KAT is affected in mouse models for research and to develop small molecule drugs for prevention and treatment of KAT-involved human diseases. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3576723 Tue, 18 May 2010 23:00:00 +0100 3576723 http://www.medworm.com/index.php?rid=3518690&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F18 Conclusion: The alternative fatty acid Gate-domain motifs are essential determinants for the activity of the human ACSL6 isoforms, which appear to act as homodimeric enzyme as well as in complex with other spliced forms. These findings provide evidences that the diversity of these enzyme species could produce the variety of acyl-CoA synthetase activities that are necessary to generate and repair the hundreds of lipid species present in membranes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3518690 Wed, 28 Apr 2010 23:00:00 +0100 3518690 http://www.medworm.com/index.php?rid=3459664&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F16 Conclusions: These analyses of UCB binding reinforce the evidence from UCB in simple systems that the pKa values of UCB are much higher than those of simple carboxylic acids. The insights derived from these analyses should be helpful to explore and interpret UCB binding to more complex, pH-sensitive, physiological moieties, such as proteins or membranes, in order to understand its functions. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3459664 Sun, 28 Mar 2010 23:00:00 +0100 3459664 http://www.medworm.com/index.php?rid=3459663&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F15 Conclusions: No reliable aqueous pKa values of UCB are available for comparison with our partition-derived results. A companion paper shows that only the high pKa values can explain the pH-dependence of UCB binding to phospholipids, cyclodextrins, and alkyl-glycoside and bile salt micelles. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3459663 Sun, 28 Mar 2010 23:00:00 +0100 3459663 http://www.medworm.com/index.php?rid=3369595&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F13 Conclusion: The biochemical and structural properties of AzoB from P. kullae K24 revealed its preference for NADPH over NADH and it is a member of the monomeric flavin-free azoreductase group. Our studies show the substrate specificity of AzoB based on structure and cofactor requirement and the phylogenetic relationship among azoreductase groups. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3369595 Tue, 16 Mar 2010 00:00:00 +0100 3369595 http://www.medworm.com/index.php?rid=3337835&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F12 Conclusions: RA muscle has some particularities in comparison to the LT and TB muscles, especially concerning the unusual large cross-section surface of SO fibres and the very low oxidative activity of intermediate IIA fibres. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3337835 Fri, 05 Mar 2010 00:00:00 +0100 3337835 http://www.medworm.com/index.php?rid=3287155&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F11 Conclusions: T. koksaghyz rubber particles are similar to those described for H. brasiliensis. They contain very pure, high molecular mass poly(cis-1,4-isoprene) and the chain elongation process can be studied ex vivo. Because of their localization on rubber particles and their activity in yeast, we propose that the recently described T. koksaghyz CPTs are the major rubber chain elongating enzymes in this species. T. koksaghyz is amenable to genetic analysis and modification, and therefore could be used as a model species for the investigation and comparison of rubber biosynthesis. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3287155 Fri, 19 Feb 2010 00:00:00 +0100 3287155 http://www.medworm.com/index.php?rid=3258174&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F10 Conclusions: This is the first biochemical characterization of conjugative transport ATPases encoded by a conjugative plasmid from Aeromonas. Our study demonstrated that the three ATPases of a newly reported TFSS of A. veronii plasmid pAc3249A are functional in both ATP hydrolysis and ATP binding. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3258174 Tue, 09 Feb 2010 00:00:00 +0100 3258174 http://www.medworm.com/index.php?rid=3230762&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F9 Conclusions: The online accessible version of the TEED has been designed to serve as a navigation and analysis tool for the large and diverse family of ThDP-dependent enzymes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3230762 Mon, 01 Feb 2010 00:00:00 +0100 3230762 http://www.medworm.com/index.php?rid=3219769&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F8 Conclusions: The phosphate sites at the subunit-subunit interface of C. callunae starch phosphorylase appear to be cooperatively functional in conferring extra kinetic stability to the native dimer structure of the active enzyme. The molecular strategy exploited for quaternary structure stabilization is to our knowledge novel among dimeric proteins. It can be distinguished clearly from the co-solute effect of orthophosphate on protein thermostability resulting from (relatively weak) interactions of the ligand with protein surface residues. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3219769 Fri, 29 Jan 2010 00:00:00 +0100 3219769 http://www.medworm.com/index.php?rid=3215739&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F5 Conclusion: This set of assays is rapid and simple to use and can immediately detect even partial defects, as the activity of each enzyme can be readily compared with one or more other activities measured in the same sample. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3215739 Thu, 28 Jan 2010 00:00:00 +0100 3215739 http://www.medworm.com/index.php?rid=3215738&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F7 Conclusions: These changes in the interaction derived from the phosphorylation state of NuMA1 suggest that phosphorylation can act as subtle mechanism of temporal and spatial regulation of the complex 4.1R-NuMA1 and therefore of the processes where both proteins play a role. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3215738 Thu, 28 Jan 2010 00:00:00 +0100 3215738 http://www.medworm.com/index.php?rid=3175162&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F3 Conclusions: There is increasing evidence that the thioredoxin and glutathione redox systems have overlapping functions and these present data indicate that the thioredoxin system plays a key role in regulating the modification of proteins by the glutathione system. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3175162 Thu, 14 Jan 2010 00:00:00 +0100 3175162 http://www.medworm.com/index.php?rid=3152815&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F2 Conclusions: Both Tyr985 and Tyr1077 contribute to the negative regulation of LEPRb signaling. The inhibitory effects of SOCS1 and SOCS3 differ in the dependence on the tyrosine residues in the intracellular domain of LEPRb. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3152815 Fri, 08 Jan 2010 00:00:00 +0100 3152815 http://www.medworm.com/index.php?rid=3138708&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F11%2F1 Conclusion: This paper represents the first report of a bifunctional regulatory enzyme catalysing an ADP-dependent phosphorylation and a Pi-dependent pyrophosphorylation reaction in bacteria. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3138708 Sun, 03 Jan 2010 00:00:00 +0100 3138708 http://www.medworm.com/index.php?rid=3135223&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F36 Conclusion: These novel ERalpha phosphorylation sites represent new means for modulation of ERalpha activity. S559 represents the first phosphorylation site identified in the extreme C-terminus (F domain) of a steroid receptor. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3135223 Thu, 31 Dec 2009 00:00:00 +0100 3135223 http://www.medworm.com/index.php?rid=3133150&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F35 Conclusions: Identification of the A-domains as IDPs will be important for determining their precise function(s), and suggests a role in protein-protein interactions, which may explain how these proteins serve as receptors for such a wide variety of preprotein substrates. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3133150 Wed, 30 Dec 2009 00:00:00 +0100 3133150 http://www.medworm.com/index.php?rid=3104471&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F33 Conclusion: The FGFRL1 gene has evolved much earlier than previously assumed. A comparison of the intracellular domain between sea urchin and human FGFRL1 provides interesting insights into the shaping of a novel zinc binding domain. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3104471 Fri, 18 Dec 2009 00:00:00 +0100 3104471 http://www.medworm.com/index.php?rid=3096007&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F32 Conclusions: Considering different scenarios of MetAP activation by one or two metal ions, it is concluded that E. coli MetAP is fully active as a monometalated enzyme. Our approach can be of value in proper determination of the number of cations needed for catalysis by metalloenzymes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3096007 Thu, 17 Dec 2009 00:00:00 +0100 3096007 http://www.medworm.com/index.php?rid=3088654&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F31 Conclusions: Our data are consistent with the formation of a substrate carbocation intermediate and cation-pi stabilization of this intermediate by two aromatic residues in the substrate-binding cleft of BCMO1. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3088654 Mon, 14 Dec 2009 00:00:00 +0100 3088654 http://www.medworm.com/index.php?rid=3084301&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F30 Conclusions: This study reveals the structural diversity of GRLN and GRLN-LP in vertebrates. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3084301 Mon, 14 Dec 2009 00:00:00 +0100 3084301 http://www.medworm.com/index.php?rid=3039135&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F29 Conclusions: This study identified the putative PrP core binding domain that facilitates the PrPC-PrPSc interaction (not conversion), likely important in the species-barrier and/or scrapie susceptibility. The octarepeats can be involved in PrPC-PrPSc stabilization, whereas the N-terminal glycosaminoglycan binding motif and the amyloidogenic motif indirectly affected conversion. Binding domain 2 and the C-terminal domain are directly implicated in PrPC self-interaction during the conversion process and may prove to be prime targets in new therapeutic strategy development, potentially retaining PrPC function. These results emphasize the importance of probable PrPC-PrPC and required PrPC-PrPSc interactions during PrP conversion. All interactions are probably part of the complex process in whi... BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=3039135 Mon, 30 Nov 2009 00:00:00 +0100 3039135 http://www.medworm.com/index.php?rid=2988329&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F27 Conclusions: The online accessible version of the CYPED at http://www.cyped.uni-stuttgart.de provides a valuable tool for the analysis of sequences, structures and their relationships to biochemical properties. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2988329 Thu, 12 Nov 2009 00:00:00 +0100 2988329 http://www.medworm.com/index.php?rid=2934078&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F26 Conclusions: The results show that no additional syringolin A-specific genes were needed for the biosynthesis of the enigmatic ureido group joining two amino acids. They reveal the source of the ureido carbonyl group to be bicarbonate/carbon dioxide, which we hypothesize is incorporated by carbamylation of valine mediated by the sylC gene product(s). A similar mechanism may also play a role in the biosynthesis of other ureido-group-containing NRPS products known largely from cyanobacteria. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2934078 Wed, 28 Oct 2009 00:00:00 +0100 2934078 http://www.medworm.com/index.php?rid=2908024&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F25 Conclusions: Ion transport data for L157, S170, and S176, along with the mobility of the spin-labels, structural characterization of the resulting proteins, and toxicity assays against a target insect, suggest that the toxin undergoes conformational changes upon protein translocation into the midgut membrane. These conformational changes result in the midregion of the alpha-helix 5 being exposed to a hydrophobic-like environment. The location of these three residues in the toxin suggests that the entire alpha-helix becomes inserted in the insect midgut membrane. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2908024 Sun, 18 Oct 2009 23:00:00 +0100 2908024 http://www.medworm.com/index.php?rid=2901360&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F24 Conclusions: The conserved SAPS domain in PP6R3 forms helical repeats similar to those in golgin p115 and negatively charged residues in interhelical loops are used to associate specifically with PP6. The results advance understanding of how distinctive helical repeat subunits uniquely distribute and differentially regulate closely related Ser/Thr phosphatases. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2901360 Thu, 15 Oct 2009 23:00:00 +0100 2901360 http://www.medworm.com/index.php?rid=2818904&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F23 Conclusions: The results revealed distinct cleavage patterns at all conditions analyzed, indicating compartment-specific processing of thyroglobulin by cysteine cathepsins. In particular, proteolytic activity of cathepsin S towards the substrate thyroglobulin can now be understood as instrumental for extracellular thyroid hormone liberation. Our study emphasizes that the proteolytic functions of cysteine cathepsins in the thyroid are not restricted to endo-lysosomes but include pivotal roles in extracellular substrate utilization. We conclude that understanding of the interplay and fine adjustment of protease networks in vivo is better approachable by simulating physiological conditions in protease activity assays. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2818904 Mon, 21 Sep 2009 23:00:00 +0100 2818904 http://www.medworm.com/index.php?rid=2729450&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F22 Conclusions: This study demonstrated that the 318PKKGDKYDKTD328 peptides localized in the C-terminal conserved domain of PSRPK with the Omega-loop structure could play a crucial role in the NLS function of PSRPK. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2729450 Mon, 24 Aug 2009 23:00:00 +0100 2729450 http://www.medworm.com/index.php?rid=2503489&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F20 Conclusions: The implication of the glyoxylate bypass in the pathology of various microorganisms makes malate synthase G an attractive new target for antibacterial therapy. The purification procedure and biochemical characterization assist in the development of antibacterial components directed against this target in P. aeruginosa. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2503489 Tue, 23 Jun 2009 23:00:00 +0100 2503489 http://www.medworm.com/index.php?rid=2503490&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F19 Conclusion: The substrate specificities are altered in V45A, Q182L, and V45A/Q182L EcMutY mutants. V45A and Q182L mutants had reduced binding and glycosylase activities for A/G and A/8-oxoG mismatches and increased affinities towards T/G mismatch. However, in contrast to a previous report that Mig.MthI thymine DNA glycosylase can be converted to a MutY-like adenine glycosylase by replacing two residues (A50V and L187Q), both V45A and Q182L EcMutY mutants did not exhibit any T/G or T/8-oxoG glycosylase activity. The dominant negative phenotype of V45A EcMutY mutant protein is probably caused by its increased binding affinity to T/G mismatch and thus inhibiting other repair pathways. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2503490 Thu, 11 Jun 2009 23:00:00 +0100 2503490 http://www.medworm.com/index.php?rid=2503492&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F17 Conclusion: We have characterised the DNA binding kinetics of the two regulators PlnC and PlnD from the bacteriocin locus in L. plantarum C11. Our data show that PlnC and PlnD, despite their strong homology to each other, differ greatly from each other in terms of binding affinity and cooperativity to the different promoters of the pln regulon. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2503492 Wed, 10 Jun 2009 23:00:00 +0100 2503492 http://www.medworm.com/index.php?rid=2503491&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F18 Conclusions: Recombinant M. tuberculosis DNA topoisomerase I can be expressed as a soluble protein and purified in high yield from E. coli host with a new protocol. Analysis of DNA cleavage with M. tuberculosis DNA substrate showed that the preferred DNA cleavage sites have a C nucleotide in the -4 position. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2503491 Wed, 10 Jun 2009 23:00:00 +0100 2503491 http://www.medworm.com/index.php?rid=2521662&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F16 Conclusion: Together, our study introduces a novel transcriptional regulator and its function in cooperation with chromatin associated factors and the basal transcription machinery. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2521662 Tue, 09 Jun 2009 23:00:00 +0100 2521662 http://www.medworm.com/index.php?rid=2446197&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F15 Conclusions: At least two distinct NatA protein N-alpha-terminal acetyltransferases coexist in human cells potentially creating a more complex and flexible system for N-alpha-terminal acetylation as compared to lower eukaryotes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2446197 Fri, 29 May 2009 04:00:00 +0100 2446197 http://www.medworm.com/index.php?rid=2426893&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F14 Conclusions: These data support the hypothesis that the signaling lipid NAGly is a metabolic product of AEA by both oxidative metabolism of the AEA ethanolamine moiety and through the conjugation of glycine to AA that is released during AEA hydrolysis by FAAH. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2426893 Thu, 21 May 2009 04:00:00 +0100 2426893 http://www.medworm.com/index.php?rid=2422660&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F13 Conclusion: The findings presented here suggest that the properties of these two orthologous proteins are similar and that conclusions reached using the bovine TAFI may be extrapolated to the human protein. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2422660 Tue, 05 May 2009 04:00:00 +0100 2422660 http://www.medworm.com/index.php?rid=2396692&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F13 Conclusions: These findings suggest that the properties of these two orthologous proteins are similar and that conclusions reached using the bovine TAFI may be extrapolated to the human protein. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2396692 Tue, 05 May 2009 04:00:00 +0100 2396692 http://www.medworm.com/index.php?rid=2363615&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F12 Conclusions: Thus, the cumulative results identified ALIX as an ubiquitination substrate of POSH and indicate that POSH and ALIX cooperate to facilitate efficient virus release. However, while ALIX is obligatory for the release of YPXnL-dependent HIV-1, POSH, albeit rate-limiting, may be functionally interchangeable. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2363615 Fri, 24 Apr 2009 04:00:00 +0100 2363615 http://www.medworm.com/index.php?rid=2325205&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F10 Conclusions: This type of analysis allowed us to assess the relative affinities of SET/TAF-Ibeta for different histones and identify the domains of the protein required for effective histone recognition. Our findings are consistent with recent structural studies of SET/TAF-Ibeta and can be valuable to understand the role of SET/TAF-Ibeta in chromatin function. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2325205 Thu, 09 Apr 2009 04:00:00 +0100 2325205 http://www.medworm.com/index.php?rid=2325207&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F9 Conclusions: We conclude that although protein synthesis inhibition likely contributes to cell death, it is not required. Thus, our results suggest that alpha-sarcin can promote cell death through a previously unappreciated mechanism that is independent of rRNA cleavage and JNK activation. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2325207 Fri, 03 Apr 2009 04:00:00 +0100 2325207 http://www.medworm.com/index.php?rid=2325209&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F8 Conclusions: The PST biosynthesis gene cluster presents a mosaic structure, whereby genes have apparently transposed in segments of varying size, resulting in different gene arrangements in all three sxt clusters sequenced so far. The gene cluster organizational structure and sequence similarity seems to reflect the phylogeny of the producer organisms, indicating that the gene clusters have an ancient origin, or that their lateral transfer was also an ancient event. The knowledge we gain from the characterisation of the PST biosynthesis gene clusters, including the identity and sequence of the genes involved in the biosynthesis, may also afford the identification of these gene clusters in dinoflagellates, the cause of human mortalities and significant financial loss to the tourism and shel... BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2325209 Mon, 30 Mar 2009 04:00:00 +0100 2325209 http://www.medworm.com/index.php?rid=2296321&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F7 Conclusions: The PDH bypass is active in sporophytic tissue of plants. Blocking this pathway via triple ALDH mutants does not uncover obvious visible phenotypes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2296321 Wed, 25 Mar 2009 04:00:00 +0100 2296321 http://www.medworm.com/index.php?rid=2268507&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F6 Conclusion: It appears that the F2 peptide can be used as a new potent specific GST inhibitor. It is proposed that the novel method, described in this report, might be useful for screening the inhibitors of not only GST but also other enzymes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2268507 Mon, 16 Mar 2009 04:00:00 +0100 2268507 http://www.medworm.com/index.php?rid=2089555&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F10%2F2 Conclusions: RAD52 itself being a highly aggregated protein perhaps acts as scaffold to bring together RAD51 and DNA molecules into large co-aggregates of RAD52-RAD51-DNA complexes to promote RAD51 mediated DNA unwinding reaction, when appropriate nucleotide cofactors are available, presumably through macromolecular crowding effects. Our work highlights the functional link between aggregation of protein-DNA complexes and DNA unwinding in RAD51 system. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=2089555 Fri, 09 Jan 2009 05:00:00 +0100 2089555 http://www.medworm.com/index.php?rid=1989714&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F32 Conclusions: The acetaldehyde-derived modifications in CA II molecule may have physiological consequences in alcoholic patients. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1989714 Thu, 27 Nov 2008 05:00:00 +0100 1989714 http://www.medworm.com/index.php?rid=1989715&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F31 Conclusions These findings show that the physical state of the membrane modifies drug transport and that substrate translocation is dependent on in vitro lipid composition. Multidrug transport activity seems to respond to alterations in the lateral forces exerted upon the transport proteins by the bilayer. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1989715 Tue, 25 Nov 2008 05:00:00 +0100 1989715 http://www.medworm.com/index.php?rid=1951338&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F29 Conclusions: Prp40, Luc7, and Snu71 appear to form a subcomplex within the yeast U1snRNP. Our data suggests that the N-terminal FF domains are critical for these interactions. Crystallization of Prp40, Luc7, and Snu71 have failed so far but co-crystallization of pairs or the whole tri-complex may facilitate crystallographic and further functional analysis. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1951338 Tue, 11 Nov 2008 05:00:00 +0100 1951338 http://www.medworm.com/index.php?rid=1946790&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F28 Conclusions: This modification of the microcystin-Sepharose technique offers an effective means of purifying novel PP1 regulatory subunits and provides a simple method to uncover a link between PP1 and additional cellular processes. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1946790 Mon, 10 Nov 2008 05:00:00 +0100 1946790 http://www.medworm.com/index.php?rid=1900844&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F26 Conclusion: This study applies immunoaffinity chromatography to purify BDH, the membrane-bound and lipid-dependent enzyme, as a 31 kDa single polypeptide chain. In addition, bacterial BDH isolation was achieved in a two-step purification procedure, improving the knowledge of an enzyme involved in the lipid metabolism of a unique hibernating mammal. Sequence alignment revealed conserved putative amino acids for possible NAD+ interaction. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1900844 Tue, 30 Sep 2008 04:00:00 +0100 1900844 http://www.medworm.com/index.php?rid=1840533&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F26 Conclusions: This study applies immunoaffinity chromatography to purify BDH, the membrane-bound and lipid-dependent enzyme, as a 31 kDa single polypeptide chain. In addition, bacterial BDH isolation was achieved in a two-step purification procedure, improving the knowledge of an enzyme involved in the lipid metabolism of a unique hibernating mammal. Sequence alignment revealed conserved putative amino acids for possible NAD+ interaction. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1840533 Tue, 30 Sep 2008 04:00:00 +0100 1840533 http://www.medworm.com/index.php?rid=1824354&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F25 Conclusions: We hypothesize that Stk33 is involved in the in vivo dynamics of the intermediate filament cytoskeleton by phosphorylating vimentin. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1824354 Tue, 23 Sep 2008 04:00:00 +0100 1824354 http://www.medworm.com/index.php?rid=1794016&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F23 Conclusions: These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1794016 Mon, 15 Sep 2008 04:00:00 +0100 1794016 http://www.medworm.com/index.php?rid=1679186&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F21 Conclusion: Our findings are relevant to the characterization of the molecular mechanism underlying the involvement of POB1/REPS2, SH3 and 14-3-3 proteins in receptor endocytosis, suggesting that 14-3-3 could work by bridging the EGF receptor and the scaffold protein POB1/REPS2. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1679186 Tue, 22 Jul 2008 04:00:00 +0100 1679186 http://www.medworm.com/index.php?rid=1642235&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F20 Conclusion: Two alpha-subunits (alpha1 and alpha7) of the 20S proteasome in the rpt6-1 mutant differed from their wild-type counterparts and peptidase activities were found to be lower in the mutant than in the wild-type strain. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1642235 Mon, 21 Jul 2008 04:00:00 +0100 1642235 http://www.medworm.com/index.php?rid=1575157&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F16 Conclusion: The unexpected results observed for the PLA2-BTL-2 complex strongly suggest that the pharmacological activity of this PLA2 is not solely dependent on the presence of enzymatic activity, and that other pharmacological regions may also be involved. In addition, we describe for the first time an interaction between two different molecules, which form a stable complex with significant changes in their original biological action. This opens new possibilities for understanding the function and action of crude venom, an extremely complex mixture of different molecules. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1575157 Fri, 06 Jun 2008 04:00:00 +0100 1575157 http://www.medworm.com/index.php?rid=1460746&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F14 Conclusion: We describe a new DSN from Kamchatka crab hepatopancreas, determining its primary structure and developing a preparative method for its purification. We found that DSN had unique substrate specificity, cleaving only DNA duplexes longer than 8 base pairs, or DNA in DNA-RNA hybrids. Interestingly, the DSN primary structure is homologous to well-known Serratia-like non-specific nucleases structures, but the properties of DSN are distinct. The unique substrate specificity of DSN should prove valuable in certain molecular biology applications. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1460746 Wed, 21 May 2008 04:00:00 +0100 1460746 http://www.medworm.com/index.php?rid=1406626&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F13 Conclusions: This is the first report showing that a bacterial CS is bifunctional. Primary deuterium kinetic isotope effects show that C4-proS hydrogen is being transferred during the reduction of FMNox by NADH and that hydride transfer contributes significantly to the rate-limiting step of FMN reduction reaction. Solvent kinetic isotope effects and proton inventory results indicate that proton transfer from solvent partially limits the rate of FMN reduction and that a single proton transfer gives rise to the observed solvent isotope effect. Multiple isotope effects suggest a stepwise mechanism for the reduction of FMNox. The results on enzyme kinetics described here provide evidence for the mode of action of MtCS and should thus pave the way for the rational design of antitubercular agent... BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1406626 Tue, 29 Apr 2008 04:00:00 +0100 1406626 http://www.medworm.com/index.php?rid=1390806&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F12 Conclusion: Maintenance of long term viability under the ischemic conditions in frozen muscle requires attention to the control of cellular energetics. Differential regulatory controls on AMPD by mechanisms including binding to muscle proteins, actions of allosteric effectors, glucose and temperature effects, and reversible phosphorylation adjust enzyme function for an optimal role in controlling cellular adenylate levels in ischemic frozen muscle. Stable modification of AMPD properties via freeze-responsive phosphorylation may contribute both to AMPD control and to coordinating AMPD function with other enzymes of energy metabolism in cold ischemic muscle. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1390806 Tue, 22 Apr 2008 04:00:00 +0100 1390806 http://www.medworm.com/index.php?rid=1349277&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F11 Conclusion: The new classification system provides the following features. First, the new classification system analyzes RHO enzymes as a whole. Second, the new classification system is not static but responds dynamically to the growing pool of RHO enzymes. Third, our classification can be applied reliably to the classification of incomplete RHOs. Fourth, the classification has direct applicability to experimental work. Fifth, the system provides new insights into the evolution of RHO systems based on enzyme interaction. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1349277 Thu, 03 Apr 2008 04:00:00 +0100 1349277 http://www.medworm.com/index.php?rid=1346087&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F9 Conclusions: Our results support a key role for PCs in maturation of RGMc that has implications for the physiological actions of this critical iron-regulatory protein. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1346087 Wed, 02 Apr 2008 04:00:00 +0100 1346087 http://www.medworm.com/index.php?rid=1331236&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F8 Conclusion: PQQ is derived from the two amino acids glutamate and tyrosine encoded in the precursor peptide PqqA. Five reactions are necessary to form this quinone cofactor. The PqqA peptide is recognised by PqqE, which links the C9 and C9a, afterwards it is accepted by PqqF which cuts out the linked amino acids. The next reaction (Schiff base) is spontaneous, the following dioxygenation is catalysed by an unknown enzyme. The last cyclization and oxidation steps are catalysed by PqqC. Taken together the known facts of the different proteins we assign a putative function to all six proteins in PQQ biosynthesis pathway. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1331236 Thu, 27 Mar 2008 04:00:00 +0100 1331236 http://www.medworm.com/index.php?rid=1191160&cid=s_34019_60_f&fid=34019&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2091%2F9%2F6 Conclusion: The C-terminal domain may have been acquired to anchor inulosucrase to the cell surface. Furthermore, the acquired domains in IslA interact with the catalytic core resulting in a new conformation that renders the enzyme more stable and switch the specificity from a hydrolytic to a transglycosylase mechanism. Based on these results, chimeric constructions may become a strategy to stabilize and modulate biocatalysts based on FTF activity. (Source: BMC Biochemistry) BMC Biochemistry journals http://www.medworm.com/rss/comments.php?id=1191160 Thu, 31 Jan 2008 05:00:00 +0100 1191160