BMC Cell Biology - Latest articles via MedWorm.com MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'BMC Cell Biology - Latest articles' source. Tue, 07 Feb 2012 08:48:51 +0100 Characterization of the C. elegans erlin homologue http://www.medworm.com/index.php?rid=5626290&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F13%2F2 Conclusions: Although loss of erlin function apparently causes a strong phenotype in humans, no such effect is seen in C. elegans. C. elegans erlin does not appear to be a ubiquitous major modulator of IP3 receptor activity nor does erlin appear to play a major role in ERAD. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5626290 Mon, 23 Jan 2012 05:00:00 +0100 5626290 Depletion of the actin bundling protein SM22/transgelin increases actin dynamics and enhances the tumourigenic phenotypes of cells http://www.medworm.com/index.php?rid=5605832&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F13%2F1 Conclusions: These findings suggest that depletion of SM22 could contribute to tumourigenic properties of cells. Reduction in SM22 levels would tend to promote cell survival when cells are under stress, such as in a hypoxic tumour environment, and may also contribute to increases in actin dynamics that favour metastatic potential. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5605832 Wed, 18 Jan 2012 05:00:00 +0100 5605832 Unfertilized frog eggs die by apoptosis following meiotic exit http://www.medworm.com/index.php?rid=5535498&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F56 Conclusions: The study reveals that naturally laid intact frog eggs die by apoptosis if they are not fertilized. A maternal apoptotic program is evoked in frog oocytes upon maturation and executed after meiotic exit in unfertilized eggs. The meiotic exit is required for execution of the apoptotic program in eggs. The emerging anti-apoptotic role of meiotic metaphase arrest needs further investigation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5535498 Fri, 23 Dec 2011 05:00:00 +0100 5535498 The MEK2-binding tumor suppressor hDlg is recruited by E-cadherin to the midbody ring http://www.medworm.com/index.php?rid=5521969&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F55 Conclusion: Our results suggest that like at other cell-cell junction sites, hDlg is part of a macromolecular complex of structural and signaling proteins at the midbody. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5521969 Tue, 20 Dec 2011 05:00:00 +0100 5521969 A potential role of the JNK pathway in hyperoxia-induced cell death, myofibroblast transdifferentiation and TGF-beta1-mediated injury in the developing murine lung http://www.medworm.com/index.php?rid=5513686&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F54 Conclusions: When viewed in combination, these studies demonstrate that hyperoxia-induced cell death, myofibroblast transdifferentiation, TGF-beta1- and hyperoxia-mediated pulmonary responses are mediated, at least in part, via signaling through the JNK pathway. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5513686 Thu, 15 Dec 2011 05:00:00 +0100 5513686 Impact of stirred suspension bioreactor culture on the differentiation of murine embryonic stem cells into cardiomyocytes http://www.medworm.com/index.php?rid=5502654&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F53 Conclusions: This study demonstrates that although cardiomyocyte differentiation can be achieved in stirred suspension bioreactors, the addition of medium enhancers is not adequate to force complete differentiation as fluid shear forces appear to maintain a subpopulation of cells in a transient pluripotent state. The development of successful ESC differentiation protocols within suspension bioreactors demands a more complete understanding of the impacts of shear forces on the regulation of pluripotency and differentiation in pluripotent stem cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5502654 Wed, 14 Dec 2011 05:00:00 +0100 5502654 Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells http://www.medworm.com/index.php?rid=5466820&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F52 Conclusions: These studies suggest that inhibitory phosphorylation of RLC is not a substantial regulatory mechanism, although we cannot rule out its role in other cellular processes or perhaps other types of cells or tissues in vivo. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5466820 Fri, 02 Dec 2011 05:00:00 +0100 5466820 Dynamic expression of synemin isoforms in mouse embryonic stem cells and neural derivatives http://www.medworm.com/index.php?rid=5438946&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F51 Conclusions: Our study shows a developmental stage specific regulation of synemin isoforms in ES cells and its neural derivatives. These findings represent the first evidence that synemins could potentially be useful markers for distinguishing multipotent ES cells from undifferentiated neural stem cells and more committed progenitor cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5438946 Wed, 23 Nov 2011 05:00:00 +0100 5438946 SOX4 interacts with Plakoglobin in a Wnt3a-dependent manner in prostate cancer cells http://www.medworm.com/index.php?rid=5429572&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F50 Conclusions: These data suggest that this newly identified interaction between SOX4 and plakoglobin is inhibitory and provides new insights into the role of SOX4 in key pathways in cell proliferation, development, and cancer progression. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5429572 Sat, 19 Nov 2011 05:00:00 +0100 5429572 Tyrosine phosphorylation of cortactin by the FAK-Src complex at focal adhesions regulates cell motility http://www.medworm.com/index.php?rid=5405211&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F49 Conclusions: Our results suggest that cortactin acts as a bridging molecule between actin filaments and focal adhesions. The cortactin N-terminus associates with F-actin, while its C-terminus interacts with focal adhesions. The tyrosine phosphorylation of cortactin by the FAK-Src complex modulates its interaction with FAK and increases its turnover at focal adhesions to promote cell motility. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5405211 Sun, 13 Nov 2011 05:00:00 +0100 5405211 Myosin II activity dependent and independent vinculin recruitment to the sites of E-cadherin mediated cell-cell adhesion http://www.medworm.com/index.php?rid=5378359&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F48 Conclusion: These results demonstrate that vinculin plays an active role at adherens junctions under increased tension at cell-cell contacts where vinculin recruitment occurs in a myosin II activity-dependent manner, whereas vinculin recruitment to the quiescent cell-cell junctions depends on F-actin stabilization. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5378359 Thu, 03 Nov 2011 04:00:00 +0100 5378359 Musashi1 expression cells derived from mouse embryonic stem cells can be enriched in side population isolated by fluorescence activated cell sorter http://www.medworm.com/index.php?rid=5356975&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F47 This study was to explore whether sorting SP could enrich for the Musashi1 (Msi1) positive cells from Msi1 high expression cells (Msi1high cells) derived from mouse embryonic stem cells (ESCs) in vitro. Results: In this study, Msi1high cell population derived from ESCs were stained by Hoechst 33342, and then the SP and non-SP (NSP) fractions were analyzed and sorted by fluorescence activated cell sorter. Subsequently, the expressions of Msi1 and other markers for neural and intestinal stem cells in SP and NSP were respectively detected. SP and NSP cells were hypodermically engrafted into the backs of NOD/SCID mice to form grafts. The developments of neural and intestinal epithelial cells in these grafts were investigated. SP fraction was identified and isolated from Msi1high cell populatio... BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5356975 Wed, 26 Oct 2011 04:00:00 +0100 5356975 Protein release through nonlethal oncotic pores as an alternative nonclassical secretory pathway http://www.medworm.com/index.php?rid=5332900&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F46 Conclusions: These results are consistent with a novel model of protein release termed oncotic release, in which a change in the colloidal osmotic pressure (oncotic pressure) upon serum withdrawal creates nonlethal oncotic pores in the plasma membrane through which PGK1 - and likely other nearby proteins - are released before the pores are rapidly resealed. These findings identify an alternative mechanism of release for FGF1, HIV-Tat, and galectin 1 whose reported nonclassical secretion is induced by serum withdrawal. Oncotic release may occur in routine cell biological experiments during which cells are washed with serum-free buffers or media and in pathophysiological conditions, such as edema, during which extracellular protein concentrations change. (Source: BMC Cell Biology - Latest ar... BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5332900 Tue, 18 Oct 2011 04:00:00 +0100 5332900 Masked mRNA is stored with aggregated nuclear speckles and its asymmetric redistribution requires a homolog of Mago nashi http://www.medworm.com/index.php?rid=5312308&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F45 Conclusions: A subset of masked mRNA is stored in association with nuclear speckles during the dormant phase of microspore development in M. vestita. The asymmetric distribution of specific mRNAs to spermatogenous but not sterile cells mirrors their translational activities and appears to require the EJC or EJC components. This suggests a novel role for nuclear speckles in the post-transcriptional regulation of transcripts. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5312308 Thu, 13 Oct 2011 04:00:00 +0100 5312308 Age-related changes in rat bone-marrow mesenchymal stem cell plasticity http://www.medworm.com/index.php?rid=5312309&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F44 Conclusion: The results suggest age related differences in the differentiation capacity of BM-MSCs. These changes may affect the efficacy of BM-MSCs for use in stem cell therapy. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5312309 Wed, 12 Oct 2011 04:00:00 +0100 5312309 Different Modes of State Transitions Determine Pattern in the Phosphatidylinositide-Actin System http://www.medworm.com/index.php?rid=5294853&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F42 Conclusions: The state transitions in actin organization and the reversible transition from cytoplasmic to membrane-bound PTEN are synchronized but their patterns differ. The transitions in actin organization are independent of PTEN, but when PTEN is present, they are coupled to periodic changes in the membrane-binding of this PIP3-degrading phosphatase. The PTEN oscillations are related to motility patterns of chemotaxing cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5294853 Fri, 07 Oct 2011 04:00:00 +0100 5294853 The MDCK variety pack: choosing the right strain http://www.medworm.com/index.php?rid=5294852&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F43 The MDCK cell line provides a tractable model for studying protein trafficking, polarity and junctions (tight, adherens, desmosome and gap) in epithelial cells. However, there are many different strains of MDCK cells available, including the parental line, MDCK I, MDCK II, MDCK.1, MDCK.2, superdome and supertube, making it difficult for new researchers to decide which strain to use. Furthermore, there is often inadequate reporting of strain types and where cells were obtained from in the literature. This review aims to provide new researchers with a guide to the different MDCK strains and a directory of where they can be obtained. We also hope to encourage experienced researchers to report the stain and origin of their MDCK cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5294852 Fri, 07 Oct 2011 04:00:00 +0100 5294852 Adhesion molecule periplakin is involved in cellular movement and attachment in pharyngeal squamous cancer cells http://www.medworm.com/index.php?rid=5271176&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F41 Conclusions: PPL knockdown is related to reduced cellular movement and attachment activity in association with PI3K/Akt axis suppression, rather than malignant progression in pharyngeal cancer cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5271176 Tue, 27 Sep 2011 04:00:00 +0100 5271176 Sirtuin-mediated nuclear differentiation and programmed degradation in Tetrahymena http://www.medworm.com/index.php?rid=5247824&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F40 Conclusions: Sirtuin activity is important for several functionally distinct events requiring global chromatin condensation. Our findings suggest a novel role for sirtuins in promoting programmed pycnosis by acting on chromatin destined for degradation. The sirtuin Thd14, which displays physiological-dependent differential localization within the nucleus, is a candidate for a chromatin condensation enzyme that is coupled to nuclear degradation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5247824 Wed, 21 Sep 2011 04:00:00 +0100 5247824 Characterization of Dental Pulp Stem/Stromal Cells of Huntington Monkey Tooth Germs http://www.medworm.com/index.php?rid=5220816&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F39 Conclusions: Our results suggest that DPSCs from Huntington monkeys retain ASC properties. Thus DPSCs derived from individuals with genetic disorders such as HD could be a potential source of personal stem cells for therapeutic purposes. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5220816 Mon, 12 Sep 2011 04:00:00 +0100 5220816 Repression of GW/P body components and the RNAi microprocessor impacts primary ciliogenesis in human astrocytes http://www.medworm.com/index.php?rid=5181268&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F37 Conclusions: This study confirms and extends a previously published report that GW/P bodies reside at the centrosome in U2OS cells and documents that GW/P bodies are resident at the centrosome in diverse non-malignant cells. Further, our study demonstrates that repression of key effector proteins in the post-transcriptional miRNA pathway impairs primary cilium formation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5181268 Tue, 30 Aug 2011 23:00:00 +0100 5181268 Intracellular Trafficking as a Determinant of AS-DACA Cytotoxicity in Rhabdomyosarcoma Cells http://www.medworm.com/index.php?rid=5158306&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F36 Conclusion: Taking the evidence as a whole, suggests that endosomal vesicle trafficking influences the toxicity of AS-DACA in RMS cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5158306 Tue, 23 Aug 2011 23:00:00 +0100 5158306 Differentiation of mouse bone marrow derived stem cells toward microglia-like cells http://www.medworm.com/index.php?rid=5158307&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F35 Conclusion: We conclude that in vitro-derived microglia are the phenotypic and functional equivalents to primary microglia and could be used in cell therapy. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5158307 Thu, 18 Aug 2011 23:00:00 +0100 5158307 Localization of chondromodulin-I at the feto-maternal interface and its inhibitory actions on trophoblast invasion in vitro http://www.medworm.com/index.php?rid=5158308&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F34 Conclusions: This study demonstrates the inhibitory action of ChM-I on trophoblast migration and invasion, implying the potential role of the ChM-I expression in decidual cells for the regulated tissue remodeling and angiogenesis at feto-maternal interface. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5158308 Wed, 17 Aug 2011 23:00:00 +0100 5158308 Quantitative nucleolar proteomics reveals nuclear re-organization during stress-induced senescence in mouse fibroblast http://www.medworm.com/index.php?rid=5118341&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F33 ${item.shortDescription} (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5118341 Wed, 10 Aug 2011 23:00:00 +0100 5118341 Hypoxia-mimetic agents inhibit proliferation and alter the morphology of human umbilical cord-derived mesenchymal stem cells http://www.medworm.com/index.php?rid=5118342&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F32 ${item.shortDescription} (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5118342 Mon, 08 Aug 2011 23:00:00 +0100 5118342 Polydatin up-regulates Clara cell secretory protein to suppress phospholipase A2 of lung induced by LPS in vivo and in vitro http://www.medworm.com/index.php?rid=5061950&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F31 ${item.shortDescription} (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5061950 Sun, 24 Jul 2011 23:00:00 +0100 5061950 Isolation of mouse mesenchymal stem cells with normal ploidy from bone marrows by reducing oxidative stress in combination with extracellular matrix http://www.medworm.com/index.php?rid=5007766&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F30 Conclusions: We have developed a technique that allows to reduce the number of karyotypic abnormalities for isolation of primary mMSCs and for limited culture period by combination of low oxygen, MEF-ECM, antioxidants and low density plating strategy. The effectiveness of the new combination method is demonstrated by successful generation of iPS cells from the isolated mMSCs. However, a culture system for mMSCs still is needed to prevent all the anomalies, especially after a long-term culture period. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5007766 Tue, 05 Jul 2011 23:00:00 +0100 5007766 Dynamic reorganization of flotillins in chemokine-stimulated human T-lymphocytes http://www.medworm.com/index.php?rid=4959842&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F28 Conclusions: Our data suggest that stable flotillin cap formation in the rear of polarized T-lymphocytes requires flotillin heterooligomer formation, as well as direct F-actin interactions of flotillin-2 and raft/membrane association of flotillin-2, but not -1. Our data also implicate flotillin-rich actin-dependent membrane microdomains in T-lymphocyte uropod formation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4959842 Tue, 21 Jun 2011 23:00:00 +0100 4959842 Translocation of signalling proteins to the plasma membrane revealed by a new bioluminescent procedure http://www.medworm.com/index.php?rid=4920946&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F27 Conclusion: This approach, that keeps unaffected the translocation properties of the signalling protein, can in principle be applied to any protein that, upon activation, moves from the cytosol to the plasma membrane.Thus, not only the modulation of GPCRs and RTKs can be investigated in this way, but that of all other proteins that can be recruited to the plasma membrane also independently of receptor activation.Moreover, its automated version, which can provide information about the kinetics and concentration-dependence of the process, is also applicable to high throughput screening of drugs affecting the translocation process. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4920946 Wed, 08 Jun 2011 23:00:00 +0100 4920946 Bmp-signaling balances proliferation and differentiation of muscle satellite cell descendants http://www.medworm.com/index.php?rid=4902063&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F26 Conclusion: Our data indicate that BMP signaling plays a critical role in balancing proliferation and differentiation of activated satellite cells and their descendants. Initially, BMP signals maintain satellite cells descendants in a proliferating state thereby expanding cell numbers. After cells are committed to differentiate they upregulate the expression of the BMP inhibitor Chordin thereby supporting terminal differentiation and myotube formation in a negative feedback mechanism. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4902063 Sun, 05 Jun 2011 23:00:00 +0100 4902063 Computer-based fluorescence quantification: a novel approach to study nucleolar biology. http://www.medworm.com/index.php?rid=4892377&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F25 Conclusions: Our protocols set the stage to analyze in a quantitative fashion the kinetics of shuttling nucleolar proteins, both at the single cell level as well as for a large number of cells. Moreover, the procedures described here are compatible with high throughput image acquisition and analysis using HTS automated platforms, thereby providing the basis to quantify nucleolar components and activities for numerous samples and experimental conditions. Together with the growing amount of information obtained for the nucleolar proteome, improvements in quantitative microscopy as they are described here can be expected to produce new insights into the complex biological functions that are orchestrated by the nucleolus. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4892377 Thu, 02 Jun 2011 23:00:00 +0100 4892377 The nuclear envelope localization of DYT1 dystonia torsinA-ΔE requires the SUN1 LINC complex component http://www.medworm.com/index.php?rid=5007767&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F24 Conclusions: These data suggest that the disease causing ΔE mutation promotes an association between torA and SUN1 that is distinct to the interaction between LAP1 and ATP-bound torA. This evidence for two NE-localized binding partners suggests that torA may act on multiple substrates and/or possesses regulatory co-factor partners. In addition, finding that the DYT1 mutation causes abnormal association with SUN1 implicates LINC complex dysfunction in DYT1 dystonia pathogenesis, and suggests a gain-of-function activity contributes to this dominantly inherited disease. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5007767 Mon, 30 May 2011 23:00:00 +0100 5007767 The nuclear envelope localization of DYT1 dystonia torsinA-deltaE requires the SUN1 LINC complex component http://www.medworm.com/index.php?rid=4883265&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F24 Conclusions: These data suggest that the disease causing DeltaE mutation promotes an association between torA and SUN1 that is distinct to the interaction between LAP1 and ATP-bound torA. This evidence for two NE-localized binding partners suggests that torA may act on multiple substrates and/ or possesses interacting co-factor partners that regulate torA activity. In addition, finding that the DYT1 mutation causes abnormal association with SUN1 implicates LINC complex dysfunction in DYT1 dystonia pathogenesis, and suggests a gain-of-function activity contributes to this dominantly inherited disease. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4883265 Mon, 30 May 2011 23:00:00 +0100 4883265 Immortalized cells and one oncogene in malignant transformation: old insights on new explanation http://www.medworm.com/index.php?rid=4862233&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F23 Conclusions: Thus, the experiments with established cell lines reinforce the notion that immortality is an essential requirement for malignant transformation that cooperates with other oncogenic changes to program the neoplastic state and substances under such investigation should be interpreted as factors which do not malignantly transform normal cells alone, but possess the ability to enhance the tumorigenic potential of already immortalized cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4862233 Sun, 22 May 2011 23:00:00 +0100 4862233 A Potential Role for the Clathrin Adaptor GGA in Drosophila Spermatogenesis http://www.medworm.com/index.php?rid=4847751&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F22 Background: GGAs (Golgi-localised, gamma-ear containing, ADP ribosylation factor-binding) are a family of clathrin adaptors that sort a number of biologically important transmembrane proteins into clathrin-coated vesicles. Knockout and knockdown studies to determine GGA function are confounded by the fact that there are 3 GGA genes in mammalian cells. Thus Drosophila melanogaster is a useful model system to study tissue expression profiles and knockdown phenotypes as there is a single GGA ortholog. Results: Here we have quantified protein expression in Drosophila and show that there is >3-fold higher expression of GGA in male flies relative to female flies. In female flies the majority of GGA expression is in the head. In male flies GGA is not only expressed at high levels in the head but ... BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4847751 Thu, 19 May 2011 23:00:00 +0100 4847751 u-Slide Chemotaxis: A new chamber for long-term chemotaxis studies http://www.medworm.com/index.php?rid=4841100&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F21 Conclusions: Compared to the Boyden chamber assay, this innovative system allows for the generation of a stable gradient for a much longer time period as well as for the tracking of cell locomotion along this gradient and over long distances. Finally, random migration can be distinguished from primed and directed migration along chemotactic gradients in the same experiment, a feature, which can be qualified via cell morphology imaging. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4841100 Tue, 17 May 2011 23:00:00 +0100 4841100 Surface expression and limited proteolysis of ADAM10 are increased by a dominant negative inhibitor of dynamin http://www.medworm.com/index.php?rid=4841101&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F20 Conclusions: Surface expression and limited proteolysis of ADAM10 are regulated by dynamin-dependent endocytosis, but are unaffected by activation of signaling pathways that upregulate shedding of ADAM substrates such as APP. Modulation of ADAM10 internalization could affect cellular behavior in two ways: by altering the putative signaling activity of the ADAM10 C-terminal fragment, and by regulating the biological function of ADAM10 substrates such as APP and N-cadherin. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4841101 Mon, 16 May 2011 23:00:00 +0100 4841101 AGE-BSA down-regulates endothelial connexin43 gap junctions http://www.medworm.com/index.php?rid=4828632&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F19 Conclusions: AGE-BSA down-regulated Cx43 expression in HAEC, mainly through reduced Cx43 transcription, and the process involved activation of ERK and p38 MAPK. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4828632 Sun, 15 May 2011 23:00:00 +0100 4828632 Myomegalin is a novel A-kinase anchoring protein involved in the phosphorylation of cardiac myosin binding protein C http://www.medworm.com/index.php?rid=4812964&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F18 Conclusions: This study ascribes a novel function to MMGL isoform 4: it meets all criteria for classification as an AKAP, and we show that is involved in the phosphorylation of cMyBPC as well as cTNI, hence MMGL is an important regulator of cardiac contractility. This has further implications for understanding the patho-aetiology of HCM-causing mutations in the genes encoding cMyBPC and cTNI, and raises the question of whether MMGL might itself be considered a candidate HCM-causing or modifying factor. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4812964 Mon, 09 May 2011 23:00:00 +0100 4812964 Stanniocalcin 2 alters PERK signaling and reduces cellular injury during cerulein induced pancreatitis in mice http://www.medworm.com/index.php?rid=4788899&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F17 Conclusions: This study links STC2 to the pathological activation of PERK in vivo, and suggests involvement of STC2 in responding to pancreatic acinar cell injury. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4788899 Wed, 04 May 2011 23:00:00 +0100 4788899 Quantitative Digital In Situ Senescence-Associated beta-Galactosidase Assay. http://www.medworm.com/index.php?rid=4719717&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F16 Conclusions: We conclude that quantitative in situ SABG assay, is feasible and reproducible and that the pH at which the reaction is performed should be tailored and chosen, depending on the research question and experimental system of interest. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4719717 Thu, 14 Apr 2011 23:00:00 +0100 4719717 Mitotic phosphorylation activates hepatoma-derived growth factor as a mitogen http://www.medworm.com/index.php?rid=4709047&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F15 Conclusions: Thus, HDGF is a phosphoprotein and phosphorylation of S103 is mitosis related and required for its function as a mitogen. We speculate that cell cycle regulated phosphorylation of HDGF may play an important role in vascular cell proliferation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4709047 Tue, 12 Apr 2011 23:00:00 +0100 4709047 Occurrence of multipolar mitoses and association with Aurora-A/-B kinases and p53 mutations in aneuploid esophageal carcinoma cells http://www.medworm.com/index.php?rid=4683992&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F13 Conclusions High Aurora-A expression alone is not associated with overt multipolar mitoses in aneuploid ESCC and BAC cancer cells, as specifically shown here for OE21 and OE33 cells, respectively. Additional p53 loss of function mutations are necessary for this to occur, at least for invasive esophageal cancer cells. Further assessment of Aurora kinases and p53 interactions in cells or tissue specimens derived from non-invasive dysplasia (ESCC) or intestinal metaplasia (BAC) are necessary to disclose a potential causative role of Aurora kinases and p53 for development of aneuploid, invasive esophageal cancers. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4683992 Tue, 05 Apr 2011 23:00:00 +0100 4683992 Long term culture of mesenchymal stem cells in hypoxia promotes a genetic program maintaining their undifferentiated and multipotent status. http://www.medworm.com/index.php?rid=4658180&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F12 Conclusion: The duration of hypoxemia is a critical parameter in the differentiation capacity of MSC. Even in growth promoting conditions, hypoxia enhanced a genetic program that maintained the cells undifferentiated and multipotent. This condition may better reflect the in vivo gene signature of MSC, with potential implications in regenerative medicine. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4658180 Tue, 29 Mar 2011 23:00:00 +0100 4658180 Requirement of Osteopontin in migration and the protection against Taxol-induced apoptosis via ATX-LPA axis in SGC7901 cells http://www.medworm.com/index.php?rid=4600378&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F11 Conclusions: The study provides the first evidence to show that expression of OPN induced by ATX-LPA axis was mediated by the activation of Akt and MAPK/ERK pathways through LPA2 receptor. In addition, OPN was required for the protective effect of ATX-LPA against Taxol-induced apoptosis and ATX-LPA-induced migration of SGC7901 cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4600378 Wed, 16 Mar 2011 00:00:00 +0100 4600378 Carbon monoxide prevents hepatic mitochondrial membrane permeabilization http://www.medworm.com/index.php?rid=4565746&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F10 Conclusion: CO directly prevents mitochondrial membrane permeabilization, which might be implicated in the hepatic apoptosis inhibition by this gaseoustransmitter. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4565746 Wed, 09 Mar 2011 00:00:00 +0100 4565746 Targeting the motor regulator Klar to lipid droplets http://www.medworm.com/index.php?rid=4517019&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F9 Conclusions: Our analysis demonstrates that droplet targeting of Klar occurs via a cis-acting sequence and generates a new tool for monitoring lipid droplets in living tissues of Drosophila. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4517019 Thu, 24 Feb 2011 00:00:00 +0100 4517019 Auxin-inducible protein depletion system in fission yeast http://www.medworm.com/index.php?rid=4464372&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F8 Conclusion: Improvement of the AID system allowed us to construct conditional null mutants of S. pombe. We propose that the off-AID system is the powerful method for in vivo protein-depletion in fission yeast. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4464372 Fri, 11 Feb 2011 00:00:00 +0100 4464372 Interaction of annexin A6 with alpha actinin in cardiomyocytes http://www.medworm.com/index.php?rid=4411409&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F7 Conclusions: In overall, the present study demonstrated for the first time that annexin A6 physically interacts with sarcomeric alpha actinin and alters contractility of cardiomyocytes suggesting that it might play important role in excitation and contraction process. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4411409 Fri, 28 Jan 2011 00:00:00 +0100 4411409 A role for p38 MAPK in the regulation of ciliary motion in a eukaryote http://www.medworm.com/index.php?rid=4399341&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F6 Conclusions: This study supports a role for p38 MAPK in the regulation of ciliary-beat. Given the evolutionary conservation of signalling processes and cilia structure, we hypothesize that p38 MAPK may regulate ciliary beat and beat-frequency in a variety of eukaryotes. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4399341 Wed, 26 Jan 2011 00:00:00 +0100 4399341 Macrophage colony-stimulating factor and its receptor signaling augment glycated albumin-induced retinal microglial inflammation in vitro http://www.medworm.com/index.php?rid=4394177&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F5 Conclusions: We conclude that GA induces microglial activation via the release of proinflammatory cytokines, which may contribute to the inflammatory pathogenesis of diabetic retinopathy. The increased microglial expression of M-CSF/CSF-1R not only is a response to microglial activation in diabetic retinopathy but also augments the microglial inflammation responsible for the diabetic microenvironment. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4394177 Tue, 25 Jan 2011 00:00:00 +0100 4394177 DNA moves sequentially towards the nuclear matrix during DNA replication in vivo. http://www.medworm.com/index.php?rid=4371795&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F3 Conclusions: Looped DNA moves in a sequential fashion, as if reeled in, towards the NM during DNA replication in vivo thus supporting the notion that the DNA template is pulled progressively towards the replication factories on the NM so as to be replicated. These results provide further evidence that the structural DNA loops correspond to the actual replicons in vivo. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4371795 Wed, 19 Jan 2011 00:00:00 +0100 4371795 Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication http://www.medworm.com/index.php?rid=4337751&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F12%2F2 Conclusions: Drug-induced cell cycle modulation varied not only between different cell types or following treatment with different drugs, but also between cells treated with different concentrations of the same drug or following drug addition during different phases of the cell cycle. By combining cytometry analysis with the Fucci probe, we have developed a novel assay that fully integrates the complexity of cell cycle regulation into drug discovery screens. This assay system will represent a powerful drug-discovery tool for the development of the next generation of anti-cancer therapies. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4337751 Thu, 13 Jan 2011 00:00:00 +0100 4337751 The chromosomal association/dissociation of the chromatin insulator protein Cp190 of Drosophila melanogaster is mediated by the BTB/POZ domain and two acidic regions http://www.medworm.com/index.php?rid=4302059&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F101 Conclusions: The N-terminal fragment of Cp190 containing the BTB/POZ domain and the D-rich region mediates association of Cp190 with all three types of insulator complexes and that the E-rich region of Cp190 is required for dissociation of Cp190 from chromosomes during heat-shock. The heat-shock-induced dissociation is strong evidence indicating that dissociation of the essential insulator protein Cp190 from chromosomes is regulated. Our results provide a mechanism through which activities of an insulator can be modulated by internal and external cues. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4302059 Fri, 31 Dec 2010 00:00:00 +0100 4302059 DNA damage-induced translocation of S100A11 into the nucleus regulates cell proliferation http://www.medworm.com/index.php?rid=4265308&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F100 Conclusions: These observations suggest that regulation of the subcellular distribution of S100A11 plays an important role in the DNA damage response and p21-mediated cell cycle control. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4265308 Fri, 17 Dec 2010 00:00:00 +0100 4265308 The role of hRev7, the accessory subunit of hPolzeta, in translesion synthesis past DNA damage induced by benzo[a]pyrene diol epoxide (BPDE). http://www.medworm.com/index.php?rid=4248937&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F97 Conclusions: Taken together, our results which show that hRev7 is required for TLS past BPDE-induced DNA lesions but that it is not essential for inserting nucleotides opposite such lesions suggest a role for hPolzeta in the extension step of translesion synthesis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4248937 Fri, 10 Dec 2010 00:00:00 +0100 4248937 Ligands specify estrogen receptor alpha nuclear localization and degradation. http://www.medworm.com/index.php?rid=4248936&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F98 Conclusions: Our results indicate that chemical structure of ligands directly affect the nuclear fate and protein turnover of the estrogen receptor alpha independently of their impact on transcription. These findings provide a molecular basis for the selection of antiestrogen compounds issue from pharmacological studies aimed at improving treatment of breast cancer. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4248936 Fri, 10 Dec 2010 00:00:00 +0100 4248936 Lipid droplets as ubiquitous fat storage organelles in C. elegans http://www.medworm.com/index.php?rid=4241536&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F96 Conclusions: Our results demonstrate lipid droplets as ubiquitous fat storage organelles and provide a unified explanation for previous studies on fat labelling methods in C. elegans. These results have important applications to the studies of fat storage and lipid droplet regulation in the powerful genetic system, C. elegans. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4241536 Wed, 08 Dec 2010 00:00:00 +0100 4241536 Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection http://www.medworm.com/index.php?rid=4232964&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F95 Conclusions: RNAs isolated from MEC in this manner were of very good quality for subsequent linear amplification, thus making it possible to establish a referential gene expression profile of the healthy MEC, a useful platform for tumor biomarker discovery. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4232964 Mon, 06 Dec 2010 00:00:00 +0100 4232964 Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells http://www.medworm.com/index.php?rid=4224884&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F94 Conclusion: These results provide evidence that hypoxia promotes the differentiation of human fetal neural progenitor cells, and identifies the involvement of erythropoietin during differentiation as well as different cellular mechanisms underlying the induction of differentiation mediated by lowered oxygen levels. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4224884 Thu, 02 Dec 2010 00:00:00 +0100 4224884 Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation http://www.medworm.com/index.php?rid=4219541&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F93 Conclusion: Our results reveal some new links between anoikis and cell transformation and they provide a reproducible model system which can potentially be useful to study multistage cancer and to identify new targets for drug development. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4219541 Thu, 02 Dec 2010 00:00:00 +0100 4219541 cAMP/PKA signaling balances respiratory activity with mitochondria dependent apoptosis via transcriptional regulation http://www.medworm.com/index.php?rid=4200463&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F92 Conclusions: We propose that in yeast, as is the case in mammalian cells, mitochondrial function and biogenesis are controlled in response to environmental change by the concerted regulation of multiple transcription factors. The visualization of cAMP/TPK3 induced cell death within yeast colonies supports a model that PKA regulation plays a physiological role in coordinating respiratory function and cell death with nutritional status in budding yeast. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4200463 Thu, 25 Nov 2010 00:00:00 +0100 4200463 The role of HIF-1 in up-regulating MICA expression on human renal proximal tubular epithelial cells during hypoxia/ reoxygenation http://www.medworm.com/index.php?rid=4189954&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F91 Conclusions: These results demonstrate that hypoxia/reoxygenation-promoted MICA expression on HK-2 cells is through a HIF-1 pathway. The increased IFNgamma secretion and enhanced NK cell cytotoxicity was mainly due to the surface expression of MICA induced by over-expression of HIF-1alpha. This study enhances our understanding of MICA activation mechanisms during kidney transplantation and provides insights into how IRI can influence transplant outcome. Moreover, these findings might be also important for developing strategies to reduce the effect of MICA in kidney transplant outcomes in the future. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4189954 Tue, 23 Nov 2010 00:00:00 +0100 4189954 Acetylated microtubules are required for fusion of autophagosomes with lysosomes http://www.medworm.com/index.php?rid=4189956&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F89 Conclusions: Acetylated microtubules are required for fusion of autophagosomes with lysosomes to form autolysosomes. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4189956 Mon, 22 Nov 2010 00:00:00 +0100 4189956 Structural determinants of PINK1 topology and dual subcellular distribution http://www.medworm.com/index.php?rid=4189955&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F90 Conclusion: Together, we believe that once PINK1 enters the mitochondria, PINK1 adopts a tethered topology because the transmembrane domain and the kinase domain prevent PINK1 forward movement into the mitochondria. Subsequent proteolysis downstream of the transmembrane domain then releases PINK1 for retrograde movement while PINK1 kinase interacts with Hsp90 chaperone. The significance of this dual localization could mean that PINK1 has compartmental-specific functions. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4189955 Mon, 22 Nov 2010 00:00:00 +0100 4189955 A non-BRICHOS surfactant protein C mutation disrupts epithelial cell function and intercellular signaling http://www.medworm.com/index.php?rid=4186847&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F88 Conclusion: We show that I73T mutation leads to impaired processing of proSP-C in alveolar type II cells, alters their stress tolerance and surfactant lipid composition, and activates cells of the immune system. In addition, we show that some of the mentioned cellular aspects behind the disease can be modulated by application of pharmaceutical drugs commonly applied in the ILD therapy. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4186847 Sat, 20 Nov 2010 00:00:00 +0100 4186847 The effects of low frequency electrical stimulation on satellite cell activity in rat skeletal muscle during hindlimb suspension http://www.medworm.com/index.php?rid=4179109&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F87 Conclusion: This study shows that electrical stimulation partially attenuated the decrease in muscle size and satellite cells during hindlimb unloading. The causal relationship between satellite cell activation and electrical stimulation remain to be established. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4179109 Thu, 18 Nov 2010 00:00:00 +0100 4179109 Robust patterns in the stochastic organization of filopodia http://www.medworm.com/index.php?rid=4175477&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F86 Conclusions: We find that the lognormal distribution of length and separation is robust and highly resistant to perturbation. We also find that length and separation are independent variables. Most importantly, our empirical data is not entirely in agreement with predictions made based on existing theoretical models and that filopodial size and separation are an order of magnitude larger than what existing models suggest. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4175477 Wed, 17 Nov 2010 00:00:00 +0100 4175477 Human ASPM participates in spindle organisation, spindle orientation and cytokinesis http://www.medworm.com/index.php?rid=4133351&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F85 Conclusions: These observations indicate that ASPM participates in spindle organisation, spindle positioning and cytokinesis in all dividing cells and that the extreme C-terminus of the protein is required for ASPM localisation and function. Our data supports the hypothesis that the MCPH phenotype caused by ASPM mutation is a consequence of mitotic aberrations during neurogenesis. We propose the effects of ASPM mutation are tolerated in somatic cells but have profound consequences for the symmetrical division of NPCs, due to the unusual morphology of these cells. This antagonises the early expansion of the progenitor pool that underpins cortical neurogenesis, causing the MCPH phenotype. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4133351 Tue, 02 Nov 2010 00:00:00 +0100 4133351 Mcm10 proteolysis initiates before the onset of M-phase http://www.medworm.com/index.php?rid=4118290&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F84 Conclusions: It is believed that the proteolysis of Mcm10 during mitosis is a vital mechanism to prevent aberrant initiation of replication and the present study describes the regulation of Mcm10 during this phase of the cell-cycle. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4118290 Wed, 27 Oct 2010 23:00:00 +0100 4118290 The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. II: Functional activity of cryopreserved cells http://www.medworm.com/index.php?rid=4105471&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F83 Conclusions: The means and methodologies described here enable the freezing and thawing of spatially identifiable cells, as well as the efficient detection of viable, specific, highly biologically active cells for future applications. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4105471 Sun, 24 Oct 2010 23:00:00 +0100 4105471 Spontaneously Immortalised Bovine Mammary Epithelial Cells Exhibit a Distinct Gene Expression Pattern from the Breast Cancer Cells http://www.medworm.com/index.php?rid=4097453&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F82 Conclusions: Spontaneously immortalised BME65Cs cells maintain many characteristics of normal BMEC cells and exhibit non-malignant transformation. Although this cell line displays altered patterns of gene expression, it is clearly distinct from malignant breast cancer cell line. It showed that co-inhibition of cellular senescence and mitochondrial apoptosis pathways coordinates BME65Cs cells immortalisation. Additionally, mechanisms other than gene mutation are likely to be involved in regulation of cellular functions. This study provides an insight into the relationship between cell senescence and immortalisation. BME65Cs cells will be useful in future studies of cellular senescence and tumorigenesis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4097453 Thu, 21 Oct 2010 23:00:00 +0100 4097453 A novel method to derive amniotic fluid stem cells for therapeutic purposes http://www.medworm.com/index.php?rid=4081911&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F79 Conclusions: We have created a novel hAFS cell derivation method that can produce a vast amount of high quality stem cells within a short period of time. Our technique makes possibility for providing autogenic fetal stem cells and allogeneic cells for future cell-based therapy. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4081911 Mon, 18 Oct 2010 23:00:00 +0100 4081911 PIST regulates the intracellular trafficking and plasma membrane expression of Cadherin 23. http://www.medworm.com/index.php?rid=4081910&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F80 Conclusions: PIST binds cadherin 23 via its PDZ domain and retains cadherin 23 in trans-Golgi network. MAGI-1 and harmonin can compete with PIST for binding cadherin 23 and release cadherin 23 from PIST's retention. Our finding suggests that PIST, MAGI-1 and harmonin collaborate in intracellular trafficking of cadherin 23 and regulate the plasma membrane expression of cadherin 23. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4081910 Mon, 18 Oct 2010 23:00:00 +0100 4081910 GDNF stimulates the proliferation of cultured mouse immature Sertoli cells via its receptor subunit NCAM and ERK1/2 signaling pathway http://www.medworm.com/index.php?rid=4077027&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F78 Conclusions: GDNF stimulates the proliferation of ISCs via its receptor subunit NCAM and the consequent activation of the ERK1/2 signaling pathway. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4077027 Sun, 17 Oct 2010 23:00:00 +0100 4077027 A feeder-free culture using autogeneic conditioned medium for undifferentiated growth of human embryonic stem cells: Comparative expression profiles of mRNAs, microRNAs and proteins among different feeders and conditioned media http://www.medworm.com/index.php?rid=4060360&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F76 Conclusion: The T3HDF feeder and T3HDF-conditioned medium were able to support the undifferentiated growth of hES cells, and they would be useful for drug development and toxicity testing in addition to the reduced risks of xenogeneic pathogens when used for medical applications such as cell therapies. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4060360 Mon, 11 Oct 2010 23:00:00 +0100 4060360 LKB1 tumor suppressor protein regulates actin filament assembly through Rho and its exchange factor Dbl independently of kinase activity http://www.medworm.com/index.php?rid=4060359&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F77 Conclusions: This study reveals that LKB1 regulates the actin cytoskeleton through a Dbl/Rho pathway. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4060359 Mon, 11 Oct 2010 23:00:00 +0100 4060359 CTD small phosphatase like 2 (CTDSPL2) can increase epsilon- and gamma-globin gene expression in K562 cells and CD34+ cells derived from umbilical cord blood http://www.medworm.com/index.php?rid=4053230&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F75 Conclusion: CTDSPL2 gene can obviously improve the expression of epsilon- and gamma-globin genes in K562 cells and CD34+ cells derived from UCB. Our study provides a new candidate target for effective treatment of SCD and beta-thalassemia. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4053230 Fri, 08 Oct 2010 23:00:00 +0100 4053230 Identification and functional analysis of NOL7 nuclear and nucleolar localization signals http://www.medworm.com/index.php?rid=4012815&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F74 Conclusions: These results identify the minimal sequences required for the active targeting of NOL7 to the nucleus and nucleolus. Further, this work characterizes the relative contribution of each sequence to NOL7 nuclear and nucleolar dynamics, the subnuclear constituents that participate in this targeting, and suggests a functional role for NOL7 in both compartments. Taken together, these results identify the requisite protein domains for NOL7 localization, the kinetics that drive this targeting, and suggest NOL7 may function in both the nucleus and nucleolus. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4012815 Sun, 26 Sep 2010 23:00:00 +0100 4012815 Virally and physically transgenized equine adipose-derived stromal cells as a cargo for paracrine secreted factors. http://www.medworm.com/index.php?rid=3998772&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F73 Conclusion: This study provides the methods to successfully transgenize Adipose-Derived Stromal Cells both by lentiviral vector and by transfection using optimized constructs with suitable promoters and reporter genes. In conclusion these findings provide a working platform for the delivery of potentially therapeutic proteins to the site of cells injection via transgenized Equine Adipose-Derived Stromal Cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3998772 Wed, 22 Sep 2010 23:00:00 +0100 3998772 Therapeutic angiogenesis by transplantation of induced pluripotent stem cell-derived Flk-1 positive cells http://www.medworm.com/index.php?rid=3993458&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F72 Conclusions: Direct local implantation of iPS cell-derived Flk-1+ cells would salvage tissues from ischemia. These data indicate that iPS cells could be valuable in the therapeutic induction of angiogenesis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3993458 Tue, 21 Sep 2010 23:00:00 +0100 3993458 Spindle assembly checkpoint genes reveal distinct as well as overlapping expression that implicates MDF-2/Mad2 in postembryonic seam cell proliferation in Caenorhabditis elegans http://www.medworm.com/index.php?rid=3993459&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F71 Conclusion: Our results indicate that SAC genes display different tissue-specific expression patterns during postembryonic development in C. elegans with significant co-expression in hypodermal seam cells and gut cells, suggesting that these genes have distinct as well as overlapping roles in postembryonic development that may or may not be related to their established roles in mitosis. Furthermore, we provide evidence, by monitoring seam cell lineage, that one of the checkpoint genes is required for proper postembryonic cell proliferation. Importantly, our research provides the first evidence that postembryonic cell division is more sensitive to SAC loss, in particular MDF-2 loss, than embryonic cell division. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3993459 Mon, 20 Sep 2010 23:00:00 +0100 3993459 Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos http://www.medworm.com/index.php?rid=3980583&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F70 Conclusion: Collectively, these studies indicate that the expression levels of Unc-45b must be precisely regulated to ensure normal myofibril organization. Loss or overexpression of Unc-45b leads to defective myofibril organization. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3980583 Thu, 16 Sep 2010 23:00:00 +0100 3980583 Intracellular localization and interaction of mRNA binding proteins as detected by FRET. http://www.medworm.com/index.php?rid=3972721&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F69 Conclusions: Biochemical methodologies, such as co-immunoprecipitation, and cell biological approaches such as standard confocal microscopy are useful in demonstrating the possibility of proteins and/or proteins and RNAs interacting. However, as demonstrated herein, colocalization of proteins and proteins and RNA is not always indicative of interaction. To this point, using FRET and immuno-FRET, we have demonstrated that RNA-BPs can visually colocalize without producing a FRET signal. In contrast, proteins that appear to be delimited to one or another intracellular compartment can be shown to interact when those compartments are juxtaposed. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3972721 Tue, 14 Sep 2010 23:00:00 +0100 3972721 Effect of cAMP derivates on assembly and maintenance of tight junctions in human umbilical vein endothelial cells http://www.medworm.com/index.php?rid=3942645&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F68 Conclusions: pCPT-cAMP and, to a lesser extend, 8-Br-cAMP/Na improved formation of continuous tight junction strands and decreased paracellular permeability in primary HUVEC. We concluded that under these conditions HUVEC represent a feasible in vitro model to study formation and disassembly of endothelial tight junctions and to characterize tight junction-associated proteins. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3942645 Mon, 06 Sep 2010 23:00:00 +0100 3942645 Functional characterization of the protein C A267T mutation: evidence for impaired secretion due to defective intracellular transport http://www.medworm.com/index.php?rid=3938220&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F67 Conclusions: In contrast to what has been reported for other PC mutants, intracellular degradation of A267T PC was not the main/dominant mechanism underlying the reduced intracellular and secretion levels of PC. Our results indicate that the A267T mutation most likely caused misfolding of PC, which might lead to increased retention of the mutated PC in ER. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3938220 Sun, 05 Sep 2010 23:00:00 +0100 3938220 Hypoxia stimulates the expression of macrophage migration inhibitory factor in human vascular smooth muscle cells via HIF-1alpha dependent pathway http://www.medworm.com/index.php?rid=3888928&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F66 Conclusion: Our results demonstrated that in VSMCs, hypoxia increased MIF gene expression and protein production. The hypoxia-induced HIF-1 alpha activation, reactive oxygen species (ROS) generation and ERK activation might be involved in this response. Both MIF and HIF-1alpha mediated the hypoxia response of vascular smooth muscle cells, including cell migration and proliferation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3888928 Thu, 19 Aug 2010 23:00:00 +0100 3888928 AlphaS1-casein, which is essential for efficient ER-to-Golgi casein transport, is also present in a tightly membrane-associated form http://www.medworm.com/index.php?rid=3861820&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F65 Conclusions: These experiments reveal for the first time the existence of a membrane-associated form of alphaS1-casein in the endoplasmic reticulum and in more distal compartments of the secretory pathway of mammary epithelial cells. Our data suggest that alphaS1-casein, which is required for efficient export of the other caseins from the endoplasmic reticulum, plays a key role in early steps of casein micelle biogenesis and casein transport in the secretory pathway. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3861820 Wed, 11 Aug 2010 23:00:00 +0100 3861820 HMGA1 down-regulation is crucial for chromatin composition and a gene expression profile permitting myogenic differentiation http://www.medworm.com/index.php?rid=3857979&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F64 Conclusions: Our data demonstrate that HMGA1 down-regulation after induction is required to initiate the myogenic program in C2C12 cells. Sustained HMGA1a expression after induction prevents expression of key myogenic factors. This may be due to specific gene regulation and/or global effects on chromatin. Our data further corroborate that altered HMGA1 levels influence the expression of other chromatin proteins. Thus, HMGA1 is able to establish a specific chromatin composition. This work contributes to the understanding of how differential HMGA1 expression is involved in chromatin organization during cellular differentiation processes and it may help to comprehend effects of HMGA1 over-expression occurring in malign or benign tumours. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3857979 Tue, 10 Aug 2010 23:00:00 +0100 3857979 Karyopherin alpha7 (KPNA7), a divergent member of the importin alpha family of nuclear import receptors http://www.medworm.com/index.php?rid=3854424&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F63 Conclusion: KPNA7 is a novel importin alpha family member in humans that belongs to the importin alpha 2 subfamily. KPNA7 shows different subcellular localization and NLS binding characteristics compared to other members of the importin alpha family. These properties suggest that KPNA7 could be specialized for interactions with select NLS-containing proteins, potentially impacting developmental regulation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3854424 Tue, 10 Aug 2010 23:00:00 +0100 3854424 Lamellipodium extension and membrane ruffling require different SNARE-mediated trafficking pathways http://www.medworm.com/index.php?rid=3854425&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F62 Conclusions: Our findings suggest that different SNARE-mediated trafficking pathways support membrane remodeling during ECM-induced lamellipodium extension and PMA-induced ruffle formation, pointing to important mechanistic differences between these processes. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3854425 Mon, 09 Aug 2010 23:00:00 +0100 3854425 Octamer-binding factor 6 (Oct-6/Pou3f1) is induced by interferon and contributes to dsRNA-mediated transcriptional responses http://www.medworm.com/index.php?rid=3826830&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F61 Conclusion: Our data show that Oct-6 expression is not as restricted as previously assumed. Induction of Oct-6 by IFNs and viruses in at least two different cell types, and involvement of Oct-6 in gene regulation after dsRNA treatment, suggest novel functions of Oct-6 in innate immune responses. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3826830 Wed, 04 Aug 2010 23:00:00 +0100 3826830 Multiple domains in the Crumbs Homolog 2a (Crb2a) protein are required for regulating rod photoreceptor size http://www.medworm.com/index.php?rid=3802284&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F60 Conclusions: Our observations suggest that particular domains in Crb2a regulate its localization and thus may regulate its regionalized function. Our results also suggest that the PDZ-binding domain in Crb2a might bring a protein(s) into the Crb complex that alters the function of the FERM-binding domain. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3802284 Wed, 28 Jul 2010 23:00:00 +0100 3802284 Endoplasmic reticulum stress response in an INS-1 pancreatic beta-cell line with inducible expression of a folding-deficient proinsulin http://www.medworm.com/index.php?rid=3790481&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F59 Conclusions: The inducible mutant insulin expressing cell model has allowed for the identification of the ER stress response in beta-cells and the repertoire of genes/proteins induced is unique to this cell type. ER-associated degradation is essential in maintaining cell survival in cells expressing mutant insulin. This cell model will be useful for the molecular characterization of ER stress-induced genes. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3790481 Sun, 25 Jul 2010 23:00:00 +0100 3790481 Peptide aptamers as new tools to modulate clathrin-mediated internalisation - inhibition of MT1-MMP internalisation http://www.medworm.com/index.php?rid=3784068&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F58 Conclusions: Taken together, our results provide further evidence that peptide aptamers can be used to dissect molecular events mediated by individual protein domains, in contrast to the pleiotropic effects of RNA interference techniques. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3784068 Thu, 22 Jul 2010 23:00:00 +0100 3784068 Maged1, a new regulator of skeletal myogenic differentiation and muscle regeneration http://www.medworm.com/index.php?rid=3772060&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F57 Conclusions: These data demonstrate for the first time that Maged1 is an important factor required for proper skeletal myoblast differentiation and muscle healing. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3772060 Mon, 19 Jul 2010 23:00:00 +0100 3772060 Hydrogen peroxide stimulates nuclear import of the POU homeodomain protein Oct-1 and its repressive effect on the expression of Cdx-2 http://www.medworm.com/index.php?rid=3761305&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F56 Conclusion: These observations suggest that Oct-1 functions as a sensor for both metabolic and stress/survival signaling pathways via altering its nuclear-cytoplasmic shuttling. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3761305 Thu, 15 Jul 2010 23:00:00 +0100 3761305 Characterization of Diaphanous-related formin FMNL2 in human tissues http://www.medworm.com/index.php?rid=3757742&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F55 Conclusion: FMNL2 is expressed in multiple human tissues, not only in the central nervous system. The expression is especially strong in gastrointestinal and mammary epithelia, lymphatic tissues, placenta, and in the reproductive tract. In cultured melanoma cells, FMNL2 co-localizes with F-actin dots at the tips of cellular protrusions. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3757742 Wed, 14 Jul 2010 23:00:00 +0100 3757742 The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. I: Methodology http://www.medworm.com/index.php?rid=3732970&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F54 Conclusions: The results of the present study offer new opportunities for cryopreservation. Using the present methodology, the cryopreservation of individual identifiable cells, and their observation and retrieval, at an individual cell resolution become possible for the first time. This approach facilitates the correlation between cell characteristics before and after the freezing - thawing cycle. Thus, it is expected to significantly enhance current cryopreservation procedures for successful regenerative and reproductive medicine. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3732970 Tue, 06 Jul 2010 23:00:00 +0100 3732970 Arecoline induced disruption of expression and localization of the tight junctional protein ZO-1 is dependent on the HER 2 expression in human endometrial Ishikawa cells http://www.medworm.com/index.php?rid=3729741&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F53 Conclusion: Arecoline is known to induce precancerous lesions and cancer in the oral cavity of betel nut users. The arecoline down-regulation of ZO-1 expression and subcellular distribution suggests that arecoline potentially disrupts cell-cell interactions mediated by ZO-1, which may play a role in arecoline-mediated carcinogenesis. Furthermore, our study has uncovered the dependency of ZO-1 localization and expression on HER2 expression, which has therefore established a new cellular link between HER2 mediated signaling and apical junction formation involving ZO-1. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3729741 Mon, 05 Jul 2010 23:00:00 +0100 3729741 Monoclonal antibody 4C5 prevents activation of MMP2 and MMP9 by disrupting their interaction with extracellular HSP90 and inhibits formation of metastatic breast cancer cell deposits http://www.medworm.com/index.php?rid=3726464&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F51 Conclusion: Both isoforms of HSP90 are secreted by MDAMB453 cells and interact with the MMP2 and MMP9. MAb 4C5 prevents MMP2 and MMP9 activation, by disrupting their interaction with HSP90. Finally mAb 4C5 significantly inhibits the metastatic deposit formation of MDAMB453 cells, by preventing their extravasation and infiltration in the lung tissue and therefore it could be used as a potential therapeutic agent for cancer metastasis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3726464 Sun, 04 Jul 2010 23:00:00 +0100 3726464 Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels http://www.medworm.com/index.php?rid=3718211&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F50 Conclusion: The lung contains large numbers of EPCs that display commitment for both types of vessels, suggesting that lung blood and lymphatic endothelial cells are derived from a single progenitor cell. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3718211 Wed, 30 Jun 2010 23:00:00 +0100 3718211 Transdifferentiation-inducing HCCR-1 oncogene http://www.medworm.com/index.php?rid=3713951&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F49 Conclusion: Therefore, we propose that HCCR-1 may be a regulatory factor that stimulates morphogenesis of epithelia or mesenchyme during neoplastic transformation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3713951 Tue, 29 Jun 2010 23:00:00 +0100 3713951 Yeast karyopherin Kap95 is required for cell cycle progression at Start http://www.medworm.com/index.php?rid=3710406&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F47 Conclusions: Kap95 plays an essential role at the initiation of the cell cycle by driving the nuclear import of Swi4, Swi6 and Mbp1, the three transcription factors responsible for the gene expression at Start. This transport depends on the specific nuclear localization signals present in cargo proteins. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3710406 Mon, 28 Jun 2010 23:00:00 +0100 3710406 Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters http://www.medworm.com/index.php?rid=3699386&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F46 Conclusions: This is the first study to demonstrate that dermal fibroblasts can differentiate into insulin-producing cells without genetic manipulation. This may offer a safer cell source for future stem cell-based therapies. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3699386 Thu, 24 Jun 2010 23:00:00 +0100 3699386 An autocrine sphingosine-1-phosphate signaling loop enhances NF-kappaB-activation and survival http://www.medworm.com/index.php?rid=3690713&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F45 Conclusions: We propose a self-amplifying loop of autocrine S1P with capacity to enhance cell survival. The mechanism provides increased understanding of the multifaceted roles of S1P in regulating cell fate during normal development and carcinogenesis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3690713 Wed, 23 Jun 2010 23:00:00 +0100 3690713 Crosstalks between integrin alpha 5 and IGF2/IGFBP2 signalling trigger human bone marrow-derived mesenchymal stromal osteogenic differentiation http://www.medworm.com/index.php?rid=3690714&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F44 Conclusion: The results show that ITGA5 is a key mediator of IGF2 and IGFBP2 expression that promotes osteoblast differentiation in human MSCs, and reveal that crosstalks between ITGA5 and IGF2/IGFBP2 signalling are important mechanisms that trigger osteogenic differentiation in human bone marrow derived mesenchymal stromal cells. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3690714 Tue, 22 Jun 2010 23:00:00 +0100 3690714 The methylated N-terminal tail of RCC1 is required for stabilisation of its interaction with chromatin by Ran in live cells http://www.medworm.com/index.php?rid=3683416&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F43 Conclusions: These results show that the stabilisation of the dynamic interaction of RCC1alpha with chromatin by Ran in live cells requires the N-terminal tail of RCC1alpha. alpha-N-methylation is not regulated by formation of the binary complex with Ran, but it promotes chromatin binding through the tail. This work supports a model in which the association of RCC1alpha with chromatin is promoted by a conformational change in the alpha-N-terminal methylated tail that is induced allosterically in the binary complex with Ran. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3683416 Sun, 20 Jun 2010 23:00:00 +0100 3683416 Multiple mesodermal lineage differentiation of Apodemus sylvaticus embryonic stem cells in vitro http://www.medworm.com/index.php?rid=3676403&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F42 Conclusions: The appropriate reagents and culture milieu used in mesodermal differentiation of mouse ES cells also guide the differentiation of in vitro AS-ES1 cells into distinct mesoderm-derived cells. This study provides a better understanding of the characteristics of AS-ES1 cells, a new species ES cell line and promotes the use of Apodemus ES cells as a complement to mouse ES cells in future studies. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3676403 Fri, 18 Jun 2010 23:00:00 +0100 3676403 RNF122: A novel ubiquitin ligase associated with calcium-modulating cyclophilin ligand http://www.medworm.com/index.php?rid=3671494&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F41 Conclusions: RNF122 can be characterized as a C3H2C3-type RING finger-containing E3 ubiquitin ligase localized to the ER. RNF122 promotes its own degradation in a RING finger- and proteasome-dependent manner. RNF122 interacts with CAML, and its E3 ubiquitin ligase activity was noted to be dependent on the RING finger domain. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3671494 Wed, 16 Jun 2010 23:00:00 +0100 3671494 Heme and non-heme iron transporters in non-polarized and polarized cells http://www.medworm.com/index.php?rid=3629415&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F39 Conclusions: HCP1 and DMT1A-I are localized to the apical membrane, and HRG-1 to the basolateral membrane and lysosome. These findings suggest that HCP1 and DMT1A-I have functions in the uptake of dietary heme and non-heme iron. HRG-1 can transport endocytosed heme from the lysosome into the cytosol. These localization studies support a model in which cytosolic heme can be degraded by HOs, and the resulting iron is exported into tissue fluids via the iron transporter ferroportin 1, which is expressed in the basolateral membrane in enterocytes or in the plasma membrane in macrophages. The liberated iron is transported by transferrin and reutilized for hemoglobin synthesis in the erythroid system. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3629415 Thu, 03 Jun 2010 23:00:00 +0100 3629415 Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopy http://www.medworm.com/index.php?rid=3625305&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F38 Conclusions: LD in pre-implantation mouse embryos show a previously unappreciated complexity of behaviour that is dependent not only on microtubules, but also microfilaments. Unlike LD in other systems, LD in the mouse embryo do not fuse but form aggregates. This study establishes HGM with adaptive optics as a powerful tool for the study of LD biology and provides insights into the photo-toxic effects of imaging embryos. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3625305 Wed, 02 Jun 2010 23:00:00 +0100 3625305 Angiotensin II upregulates the expression of placental growth factor in human vascular endothelial cells and smooth muscle cells http://www.medworm.com/index.php?rid=3599263&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F36 Conclusion: Our results showed for the first time that Ang II could induce the gene expression and protein production of PlGF in VECs and VSMCs, which might play an important role in the pathogenesis of vascular inflammation and atherosclerosis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3599263 Tue, 25 May 2010 23:00:00 +0100 3599263 Connective Tissue Growth Factor (CTGF/CCN2) enhances lactogenic differentiation of mammary epithelial cells via integrin-mediated cell adhesion http://www.medworm.com/index.php?rid=3595476&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F35 Conclusions: CTGF/CCN2 expression in HC11 cells led to an increase in multiple markers of lactogenic differentiation. The mechanisms by which CTGF/CCN2 contributed to lactogenic differentiation include direct binding of CTGF/CCN2 to integrin complexes and CTGF/CCN2-induced matrix protein expression resulting in elevated integrin functionality. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3595476 Sun, 23 May 2010 23:00:00 +0100 3595476 Centriole movements in mammalian epithelial cells during cytokinesis http://www.medworm.com/index.php?rid=3585362&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F34 Conclusions: These findings show that centrioles are highly mobile during cytokinesis and suggest that the repositioning of a centriole to the intercellular bridge is not essential for controlling abscission. We suggest that centriole movements are microtubule dependent and that abscission is more dependent on other mechanisms than positioning of centrioles. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3585362 Thu, 20 May 2010 23:00:00 +0100 3585362 The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein http://www.medworm.com/index.php?rid=3563848&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F33 Conclusions: Taken together our data indicate that galactosylation of collagenous proteins by the soluble GLT25D1 occurs in the early secretory pathway. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3563848 Thu, 13 May 2010 23:00:00 +0100 3563848 Salvianolic acid B prevents epithelial-to-mesenchymal transition through the TGF-beta1 signal transduction pathway in vivo and in vitro http://www.medworm.com/index.php?rid=3533696&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F31 Conclusions: Sal B can prevent tubular EMT in the fibrotic kidney induced by HgCl2 as well as HK-2 cells triggered by TGF-b1, the mechanism of Sal B is closely related to the regulation of TGF-b1/Smads pathway, manifested as the inhibition of TGF-b1 expression, suppression of TbR-I expression and function, down-regulation of Smad2/3 phosphorylation, and restoration of the down-regulation of Smad7, as well as inhibition of MMP-2 activity. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3533696 Tue, 04 May 2010 23:00:00 +0100 3533696 Mesenchymal stem cells rescue cardiomyoblasts from cell death in an in vitro ischemia model via direct cell-to-cell connections http://www.medworm.com/index.php?rid=3486824&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F29 Conclusion: Based on these results we hypothesize that mesenchymal stem cells may reduce the number of dead cardiomyoblasts after ischemic damage via direct cell-to-cell interactions and intercellular tubular connections may play an important role in these processes (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3486824 Mon, 19 Apr 2010 23:00:00 +0100 3486824 HAb18G/CD147 cell-cell contacts confer resistance of a HEK293 subpopulation to anoikis in an E-cadherin-dependent manner http://www.medworm.com/index.php?rid=3479560&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F27 Conclusions: Our results provide new evidence that HAb18G/CD147-mediated cell-cell contact confers anoikis resistance in an E-cadherin-dependent manner; and cell-cell contact mediated resistance to anoikis implicates PI3K pathway in a highly relevant cell model (HEK293ar). Understanding of the role of HAb18G/CD147 cell-cell contacts in anoikis resistance may help in understanding the survival of cells in anchorage-independent growth, such as cells in tumor metastasis and suspension culture produced for biomedical engineering. Our results also contribute to a better understanding of the biology of HEK293 cell spheroids, a major workhorse for producing human therapeutic agents and viral vaccines. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3479560 Fri, 16 Apr 2010 23:00:00 +0100 3479560 Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction point http://www.medworm.com/index.php?rid=3479561&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F26 Conclusions: A multivariate assay for Mcm6*, PCNA*, DNA content, and a mitotic marker provides analysis capable of estimating the fraction of pre and post-restriction point G1 cells and supports the idea that there are at least two states in G1 defined by levels of chromatin bound Mcm proteins. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3479561 Thu, 15 Apr 2010 23:00:00 +0100 3479561 Functional neural differentiation of human adipose tissue-derived stem cells using bFGF and forskolin http://www.medworm.com/index.php?rid=3475562&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F25 Conclusions: These combined results indicate that hADSCs have the same self-renewing capacity and multipotency as stem cells, and can be differentiated into functional neurons using bFGF and forskolin. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3475562 Thu, 15 Apr 2010 23:00:00 +0100 3475562 Significantly improved precision of cell migration analysis in time-lapse video microscopy through use of a fully automated tracking system http://www.medworm.com/index.php?rid=3453666&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F24 Conclusion: We demonstrate that automatic multi target tracking systems identify cell objects, follow individual cells and compute migration rates with high precision, clearly outperforming manual procedures. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3453666 Wed, 07 Apr 2010 23:00:00 +0100 3453666 CC3/TIP30 affects DNA damage repair http://www.medworm.com/index.php?rid=3448633&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F23 Conclusions: Manipulation of the cellular levels of CC3 alters expression levels and/or subcellular localization of proteins that exhibit nucleocytoplasmic shuttling. This results in altered responses to genotoxic stress and adversely affects DNA damage repair by affecting the recruitment of adequate amounts of required proteins to proper cellular compartments. Excess of cellular CC3 has a significant negative effect on DNA repair after UV and oxidant exposure, while silencing of endogenous CC3 slightly delays repair of UV-induced damage. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3448633 Tue, 06 Apr 2010 23:00:00 +0100 3448633 Functional investigations on human mesenchymal stem cells exposed to magnetic fields and labeled with clinically approved iron nanoparticles http://www.medworm.com/index.php?rid=3440621&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F22 Conclusions: In our study an innovative labeling protocol for tracking MSCs by MRI using SPIO in combination with magnetic fields was established. Both, SPIO and the static magnetic field were identified as independent factors which affect the functional biology of human MSCs. Further in vivo investigations are needed to elucidate the molecular mechanisms of the interaction of magnetic fields with stem cell biology. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3440621 Mon, 05 Apr 2010 23:00:00 +0100 3440621 Nuclear variants of bone morphogenetic proteins http://www.medworm.com/index.php?rid=3370266&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F20 Conclusions: The observation that nBmp2 localization varies throughout the cell cycle, as well as the conservation of a nuclear localization mechanism among three different BMP family members, suggests that these novel nuclear variants of BMP family proteins play an important functional role in the cell. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3370266 Mon, 15 Mar 2010 00:00:00 +0100 3370266 A novel link between Sus1 and the cytoplasmic mRNA decay machinery suggests a broad role in mRNA metabolism http://www.medworm.com/index.php?rid=3366025&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F19 Conclusions: In this study, we found genetic and biochemical association between Sus1 and components responsible for cytoplasmic mRNA metabolism. Moreover, Sus1 accumulates in discrete cytoplasmic granules, which partially co-localise with P-bodies and stress granules under specific conditions. These interactions suggest a role for Sus1 in gene expression during cytoplasmic mRNA metabolism in addition to its nuclear function. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3366025 Mon, 15 Mar 2010 00:00:00 +0100 3366025 Role of casein kinase 1 in the glucose sensor-mediated signaling pathway in yeast http://www.medworm.com/index.php?rid=3342510&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F17 Conclusion: Glucose-induced, glucose sensor-regulated degradation of Mth1 occurs in the nucleus and does not require direct interaction of the glucose sensors with Yck1/2. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3342510 Sun, 07 Mar 2010 00:00:00 +0100 3342510 Integrin-linked kinase functions as a downstream signal of platelet-derived growth factor to regulate actin polymerization and vascular smooth muscle cell migration http://www.medworm.com/index.php?rid=3302180&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F16 Conclusion: This study will provide new insights into the potential therapeutic value of modulating integrin signaling in an attempt to block or delay smooth muscle cell migration and the progression of vascular diseases. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3302180 Tue, 23 Feb 2010 00:00:00 +0100 3302180 Green-to-red photoconvertible fluorescent proteins: tracking cell and protein dynamics on standard wide-field mercury arc-based microscopes http://www.medworm.com/index.php?rid=3298169&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F15 Conclusions: We demonstrate the practicability of this technique using Dendra2 and mEos2 as monomeric, photoconvertible PAFP representatives fused to proteins with low (histone H2B), medium (gap junction channel protein connexin 43), and high (alpha-tubulin; clathrin light chain) dynamic cellular mobility as examples. Comparable efficient, irreversible green-to-red photoconversion of selected portions of cell nuclei, gap junctions, microtubules and clathrin-coated vesicles was achieved. Tracking over time allowed elucidation of the dynamic live-cycle of these subcellular structures. The advantage of this technique is that it can be performed on a standard, relatively inexpensive wide-field fluorescence microscope with mercury arc illumination. Together with previously described laser scann... BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3298169 Mon, 22 Feb 2010 00:00:00 +0100 3298169 R-Ras regulates beta1-integrin trafficking via effects on membrane ruffling and endocytosis http://www.medworm.com/index.php?rid=3287554&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F14 Conclusions: Our results suggest that integrin function involves integrin trafficking via a cycle of membrane protrusion, ruffling, and endocytosis regulated by R-Ras, providing a novel mechanism by which integrins are linked to R-Ras through control of membrane dynamics. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3287554 Thu, 18 Feb 2010 00:00:00 +0100 3287554 Role of apoptosis-inducing factor (AIF) in programmed nuclear death during conjugation in Tetrahymena thermophila http://www.medworm.com/index.php?rid=3266802&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F13 Conclusion: These observations suggest that Tetrahymena AIF plays an important role in the degradation of DNA at an early stage of PND, leading to the idea that the mitochondria-initiated apoptotic DNA degradation pathway is widely conserved in eukaryotes. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3266802 Thu, 11 Feb 2010 00:00:00 +0100 3266802 Monkey hybrid stem cells develop cellular features of Huntington's disease http://www.medworm.com/index.php?rid=3243557&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F12 Conclusions: Huntington's disease cellular features is influenced by neural developmental events.These results are the first to demonstrate that a pluripotent stem cell line is able to mimic Huntington's disease progression that parallels neural development, which could be a useful cell model for investigating the developmental impact on Huntington's disease pathogenesis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3243557 Fri, 05 Feb 2010 00:00:00 +0100 3243557 Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells http://www.medworm.com/index.php?rid=3220295&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F11 Conclusion: Physiologic oxygen tension during in vitro culture of human MSC slows down cell cycle progression and differentiation. Under physiological conditions this may keep a proportion of MSC in a resting state. Further studies are needed to analyze these aspects of MSC in tissue regeneration. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3220295 Thu, 28 Jan 2010 00:00:00 +0100 3220295 A genetic screen for modifiers of Drosophila caspase Dcp-1 reveals caspase involvement in autophagy and novel caspase-related genes http://www.medworm.com/index.php?rid=3204696&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F9 Conclusions: We identified a number of Dcp-1 modifiers that genetically interact with Dcp-1-induced cell death. Our results showing that Dcp-1 and autophagy-related genes influence each other will aid future investigations of the complicated relationships between apoptosis and autophagy. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3204696 Mon, 25 Jan 2010 00:00:00 +0100 3204696 F-Prostaglandin receptor regulates endothelial cell function via fibroblast growth factor-2 http://www.medworm.com/index.php?rid=3193592&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F8 Conclusions: Taken together, we have shown a novel mechanism whereby epithelial prostaglandin F2alpha-FP signaling regulates endothelial cell network formation and proliferation. In addition we provide novel in vitro evidence to suggest that prostaglandin F2alpha can directly regulate endothelial cell network formation but not endothelial cell proliferation. These findings have relevance for pathologies where the FP receptor is aberrantly expressed, such as endometrial adenocarcinoma, and provide in vitro evidence to suggest that targeting the FP receptor could provide an anti-angiogenic approach to reducing tumour vasculature and growth. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3193592 Thu, 21 Jan 2010 00:00:00 +0100 3193592 Nutritional control of gene expression in Drosophila larvae via TOR, Myc and a novel cis-regulatory element. http://www.medworm.com/index.php?rid=3193593&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F7 Conclusions: Our data suggest that many of the transcriptional effects of amino acids are mediated via signaling through the TOR pathway in Drosophila larvae. We also find that these transcriptional effects are mediated through at least two mechanisms: via the transcription factor Myc, and via the Motif 1 cis-regulatory element. These studies begin to elucidate a nutrient-responsive signaling network that controls metabolic gene transcription in Drosophila. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3193593 Wed, 20 Jan 2010 00:00:00 +0100 3193593 TNF-alpha Mediates Eosinophil Cationic Protein-induced Apoptosis in BEAS-2B Cells http://www.medworm.com/index.php?rid=3189006&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F6 Conclusion: In conclusion, our results have demonstrated that ECP increased TNF-alpha production in BEAS-2B cells and triggered apoptosis by caspase-8 activation through mitochondria-independent pathway. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3189006 Wed, 20 Jan 2010 00:00:00 +0100 3189006 Regulation of endocytic trafficking of transferrin receptor by optineurin and its impairment by a glaucoma-associated mutant http://www.medworm.com/index.php?rid=3185191&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F4 Conclusions: Our results suggest that optineurin regulates endocytic trafficking of transferrin receptor to the juxtanuclear region. The E50K mutant impairs trafficking at the recycling endosomes due to altered interactions with Rab8 and transferrin receptor. These results also have implications for the pathogenesis of glaucoma caused by the E50K mutation because endocytic recycling is vital for maintaining homeostasis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3185191 Tue, 19 Jan 2010 00:00:00 +0100 3185191 Modulatory effects of cAMP and PKC activation on gap junctional intercellular communication among thymic epithelial cells http://www.medworm.com/index.php?rid=3175728&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F3 Conclusions: Overall, our data indicate that cAMP and PKC intracellular pathways are involved in the homeostatic control of the gap junction-mediated communication in the thymic epithelium, exerting respectively a positive and negative role upon cell coupling. This control is phylogenetically conserved in the thymus, since it was seen in both mouse and human TEC preparations. Lastly, our work provides new clues for a better understanding of how the thymic epithelial network can work as a physiological syncytium. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3175728 Fri, 15 Jan 2010 00:00:00 +0100 3175728 Elevated endogenous expression of the dominant negative basic helix-loop-helix protein ID1 correlates with significant centrosome abnormalities in human tumor cells http://www.medworm.com/index.php?rid=3171701&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F11%2F2 Conclusions: This is the first report that shows that not only ectopic expression in tissue culture but endogenous levels of ID1 modulate centrosome numbers. Thus, our findings support the hypothesis that ID1 interferes with centrosome homeostasis, most likely contributing to genomic instability and associated tumor aggressiveness. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3171701 Thu, 14 Jan 2010 00:00:00 +0100 3171701 Model system for the analysis of cell surface expression of human ABCA1 http://www.medworm.com/index.php?rid=3111122&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F10%2F93 Conclusions: Our model system allows a quantitative detection of cell surface expression of ABCA1, screening of substrates or specific inhibitors, and investigating transport regulation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3111122 Mon, 21 Dec 2009 00:00:00 +0100 3111122 Complete reversal of epithelial to mesenchymal transition requires inhibition of both ZEB expression and the Rho pathway http://www.medworm.com/index.php?rid=3111121&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F10%2F94 Conclusions: We demonstrate that reversal of EMT requires re-establishing both epithelial transcription and structural components by sustained and independent signaling through TbetaRI and ROCK. These findings indicate that combination small molecule therapy targeting multiple kinases may be necessary to reverse disease conditions. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3111121 Mon, 21 Dec 2009 00:00:00 +0100 3111121 Oxidative stress promotes autophagic cell death in human neuroblastoma cells with ectopic transfer of mitochondrial PPP2R2B (Bbeta2) http://www.medworm.com/index.php?rid=3100617&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F10%2F91 Conclusions: Cells with ectopically expressed mitochondria-targeted regulatory subunit PPP2R2B of the holoenzyme PP2A were predisposed to autophagy and oxidative stress induced cell death showing features of apoptosis. The results promised a model for studying the mechanism and function of aberrant PPP2R2B expression in neuronal cells. The work provided a new target for understanding and prevention of neuropathogenesis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3100617 Fri, 18 Dec 2009 00:00:00 +0100 3100617 DDIT3/CHOP and the sarcoma fusion oncoprotein FUS-DDIT3/TLS-CHOP bind cyclin-dependent kinase 2 http://www.medworm.com/index.php?rid=3096659&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F10%2F89 Conclusions: We conclude that DDIT3 binds CDK2 and that many of the observed biological effects of DDIT3 may involve interaction with CDK2. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3096659 Thu, 17 Dec 2009 00:00:00 +0100 3096659 Intracellular diffusion restrictions in isolated cardiomyocytes from rainbow trout http://www.medworm.com/index.php?rid=3096658&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F10%2F90 Conclusions: The difference between fibers and cardiomyocytes suggest that results from trout heart fibers were affected by incomplete separation of the cells. However, the lower ADP-affinity of cardiomyocytes compared to isolated mitochondria indicate that intracellular diffusion restrictions are still present in trout cardiomyocytes despite their lower density of intracellular membrane structures. The lack of a creatine effect indicates that trout heart lacks mitochondrial creatine kinase tightly coupled to respiration. This argues against diffusion restriction by the outer mitochondrial membrane. These results from rainbow trout cardiomyocytes resemble those from other low-performance hearts such as neonatal rat and rabbit hearts. Thus, it seems that metabolic regulation is related to c... BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3096658 Thu, 17 Dec 2009 00:00:00 +0100 3096658 Global transcriptional response after exposure of fission yeast cells to ultraviolet light http://www.medworm.com/index.php?rid=3092537&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F10%2F87 Conclusion: There is little response in fission yeast to ultraviolet light at the transcriptional level. Amongst the genes induced or repressed after ultraviolet irradiation we found none that are likely to be involved in the G1/S checkpoint mechanism, suggesting that the checkpoint is not dependent upon transcriptional regulation. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3092537 Wed, 16 Dec 2009 00:00:00 +0100 3092537 The Golgi alpha 1,6 mannosyltransferase KlOch1p of Kluyveromyces lactis is required for Ca2+/calmodulin-based signaling and for proper mitochondrial functionality. http://www.medworm.com/index.php?rid=3089108&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F10%2F86 Conclusions: Proper functioning of the alpha-1,6-mannosyltransferase in the N-glycosylation pathway of K. lactis is required for maintaining normal calcium homeostasis; this is necessary for physiological mitochondria dynamics and functionality. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3089108 Mon, 14 Dec 2009 00:00:00 +0100 3089108 Disruption of occludin function in polarized epithelial cells activates the extrinsic pathway of apoptosis leading to cell extrusion from the monolayer without loss of transepithelial resistance http://www.medworm.com/index.php?rid=3075351&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F10%2F85 Conclusions: These observations provide strong evidence that, following disruption, occludin forms a complex with the extrinsic death receptor leading to extrusion of apoptotic cells from the epithelial monolayer. They suggest that occludin has a protective as well as a barrier forming role in epithelia; pathogenic agents which utilize this protein as an entry point into the cell might set off an apoptotic reaction allowing extrusion of the infected cell before the pathogen can gain entry to the interstitial space. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3075351 Wed, 09 Dec 2009 00:00:00 +0100 3075351 Aberrant trafficking of NSCLC-associated EGFR mutants through the endocytic recycling pathway promotes interaction with Src http://www.medworm.com/index.php?rid=3039633&cid=s_34023_171_f&fid=34023&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2121%2F10%2F84 Conclusions: The findings presented in this study show that mutant EGFRs undergo aberrant traffic into the endocytic recycling compartment which allows mutant EGFRs to engage in a preferential interaction with Src, a critical partner for EGFR-mediated oncogenesis. (Source: BMC Cell Biology - Latest articles) BMC Cell Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3039633 Mon, 30 Nov 2009 00:00:00 +0100 3039633