BMC Microbiology - Latest articles via MedWorm.com MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'BMC Microbiology - Latest articles' source. Thu, 09 Feb 2012 21:34:55 +0100 A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection http://www.medworm.com/index.php?rid=5674678&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F22 Conclusion: The transcriptomic profiles of C57BL/6 macrophages, before and after infection, were shown to be involved in the macrophage pathway of activation, which may aid in the control of L. amazonensis infection, in contrast to the profiles of CBA cells. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5674678 Thu, 09 Feb 2012 05:00:00 +0100 5674678 Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction http://www.medworm.com/index.php?rid=5674679&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F21 Conclusions: D. hafniense DCB-2's genome contains genes consistent with its abilities for dehalogenation, metal reduction, N2 and CO2 fixation, anaerobic respiration, oxygen tolerance, spore formation, and biofilm formation which make this organism a potential candidate for bioremediation at contaminated sites. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5674679 Wed, 08 Feb 2012 05:00:00 +0100 5674679 Defining the "core microbiome" of the microbial communities in the tonsils of healthy pigs http://www.medworm.com/index.php?rid=5674680&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F20 Conclusions: We have extended and supported our previous studies with 16S clone libraries, using 16S rRNA gene pyrosequencing to describe the microbial communities in tonsils of healthy pigs. We have defined a core microbiome, dominated by Pasteurellaceae, in tonsil specimens, and have also demonstrated the presence of unique minor components of the tonsillar microbiome present in each herd. We have validated the use of non-invasive tonsil brushes, in comparison to tonsil tissue, which will facilitate future studies. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5674680 Tue, 07 Feb 2012 05:00:00 +0100 5674680 Chemotaxis of Burkholderia sp. strain SJ98 towards chloronitroaromatic compounds that it can metabolise http://www.medworm.com/index.php?rid=5657253&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F19 Conclusions: Burkholderia sp. strain SJ98 exhibits metabolic transformation of, and inducible chemotaxis towards CNACs. Its chemotactic responses towards these compounds are related to its previously demonstrated chemotaxis towards NACs that it can metabolise, but it is independently inducible from its chemotaxis towards succinate or aspartate. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5657253 Wed, 01 Feb 2012 05:00:00 +0100 5657253 Opposing roles of Sigma B and Sigma B-controlled SpoVG in the global regulation of esxA in Staphylococcus aureus http://www.medworm.com/index.php?rid=5625304&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F17 Conclusion: SpoVG is a Sigma B-dependent element that fine-tunes the expression of esxA by counteracting the Sigma B-induced repressing activity of the transcriptional regulator SarA and activates esxA transcription. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5625304 Tue, 24 Jan 2012 05:00:00 +0100 5625304 Lactobacillus rhamnosus GR-1 enhances NF-kappaB activation in Escherichia coli-stimulated urinary bladder cells through TLR4 http://www.medworm.com/index.php?rid=5625306&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F15 Conclusions: The immunological outcome of E. coli challenge of bladder cells was influenced by probiotic L. rhamnosus GR 1, by enhancing the activation of NF-kappaB and TNF release. Thus the urogenital probiotic L. rhamnosus GR-1 modulated the activation of the NF-kappaB through increased levels of TLR4 on the bladder cells and altered subsequent release of cytokines from urothelial cells. By influencing immunological factors such as TLR4, important in the process of fighting pathogens, lactobacilli could facilitate pathogen recognition and infection clearance. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5625306 Sun, 22 Jan 2012 05:00:00 +0100 5625306 Proteomic profiling of Pseudomonas aeruginosa AES-1R, PAO1 and PA14 reveals potential virulence determinants associated with a transmissible cystic fibrosis-associated strain http://www.medworm.com/index.php?rid=5625305&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F16 Conclusions: Our data suggest that AES-1R expresses higher levels of proteins, such as those involved in antibiotic resistance, iron acquisition and virulence that may provide a competitive advantage during early infection in the CF lung. Identification of novel proteins associated with transmissibility and acute infection may aid in deciphering new strategies for intervention to limit P. aeruginosa infections in CF patients. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5625305 Sun, 22 Jan 2012 05:00:00 +0100 5625305 The role of MglA for adaptation to oxidative stress of Francisella tularensis LVS http://www.medworm.com/index.php?rid=5617568&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F14 Conclusions: Altogether the results show that the DeltamglA mutant exhibits all the hallmarks of a bacterium subjected to oxidative stress under aerobic conditions, indicating that MglA is required for normal adaptation of F. tularensis to oxidative stress and oxygen-rich environments. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5617568 Sat, 21 Jan 2012 05:00:00 +0100 5617568 Seroepidemiology of Klebsiella pneumoniae colonizing the intestinal tract of healthy Chinese and overseas Chinese adults in Asian countries http://www.medworm.com/index.php?rid=5617569&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F13 Conclusions: The result showed that Chinese ethnicity itself might be a major factor predisposing to intestinal colonization by serotype K1/K2 K. pneumoniae isolates. The prevalent serotype K1/K2 isolates may partially correspond to the prevalence of K. pneumoniae liver abscess in Asian countries. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5617569 Thu, 19 Jan 2012 05:00:00 +0100 5617569 Immunoproteomics based identification of thioredoxin reductase GliT and novel Aspergillus fumigatus antigens for serologic diagnosis of invasive aspergillosis http://www.medworm.com/index.php?rid=5604547&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F11 Conclusions: The immunoreactive proteins identified in this study may be helpful for the diagnosis of IA in critically ill patients. Our results indicate that TR and other immunodominant antigens have potential as biomarkers for the serologic diagnosis of invasive aspergillosis. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5604547 Wed, 18 Jan 2012 05:00:00 +0100 5604547 Dominance of multidrug resistant CC271 clones in macrolide-resistant streptococcus pneumoniae in Arizona http://www.medworm.com/index.php?rid=5604546&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F12 Conclusions: The dual-positive, multidrug-resistant S. pneumoniae clones have likely expanded by switching to non-vaccine serotypes after the heptavalent pneumococcal conjugate vaccine release, and their success limits therapy options. This upsurge could have a considerable clinical impact in Arizona. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5604546 Wed, 18 Jan 2012 05:00:00 +0100 5604546 Interleukin-8 is the single most up-regulated gene in whole genome profiling of H. pylori exposed gastric epithelial cells http://www.medworm.com/index.php?rid=5604549&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F9 Conclusion: In whole genome analysis of the epithelial response to H. pylori exposure, IL-8 demonstrated the most marked up-regulation, and was involved in many of the most important cellular response processes to the infection. There was dysregulation of apoptosis, tumor suppressor genes and oncogenes as early as in the first 24 h of H. pylori infection, which may represent early signs of gastric tumorigenesis. OMPLA+/- did not affect the acute inflammatory response to H. pylori. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5604549 Tue, 17 Jan 2012 05:00:00 +0100 5604549 Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production http://www.medworm.com/index.php?rid=5604548&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F10 Conclusions: The results of this study confirm that mgoB, mgoC, mgoA and mgoD function as a transcriptional unit and operon. While this operon is composed of four genes, only the last three are directly involved in mangotoxin production. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5604548 Tue, 17 Jan 2012 05:00:00 +0100 5604548 Characterisation of a cell wall-anchored protein of Staphylococcus saprophyticus associated with linoleic acid resistance http://www.medworm.com/index.php?rid=5594072&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F8 Conclusions: Our data indicate that SssF is a newly described and highly prevalent surface-localised protein of S. saprophyticus that contributes to resistance against the antibacterial effects of linoleic acid. SssF is a member of a protein family widely disseminated throughout the staphylococci. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5594072 Sun, 15 Jan 2012 05:00:00 +0100 5594072 Thin-film fixed-bed reactor (TFFBR) for solar photocatalytic inactivation of aquaculture pathogen Aeromonas hydrophila http://www.medworm.com/index.php?rid=5594075&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F5 Aeromonas hydrophila, an important pathogen in aquaculture systems, can be inactivated using a photocatalytic reactor coated with a thin film of titanium dioxide which increases the effectiveness of solar disinfection, demonstrating the application of solar photocatalysis in purification of aquaculture systems. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5594075 Fri, 13 Jan 2012 05:00:00 +0100 5594075 Characterization of the biotin uptake system encoded by the biotin-inducible bioYMN operon of Corynebacterium glutamicum http://www.medworm.com/index.php?rid=5594074&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F6 Conclusions: The operon bioYMN from C. glutamicum was shown to be induced by biotin limitation. Transport assays with radio-labeled biotin revealed that BioYMN functions as a biotin uptake system. Overexpression of bioYMN affected L-glutamate production triggered by biotin limitation. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5594074 Fri, 13 Jan 2012 05:00:00 +0100 5594074 Role of Mycobacterium tuberculosis pknD in the pathogenesis of Central Nervous System Tuberculosis http://www.medworm.com/index.php?rid=5594073&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F7 Conclusions: Our findings demonstrate a novel in vivo role for M. tuberculosis pknD and represent an important mechanism for bacterial invasion and virulence in central nervous system tuberculosis, a devastating and understudied disease primarily affecting young children. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5594073 Fri, 13 Jan 2012 05:00:00 +0100 5594073 Endophytic fungal association via gibberellins and indole acetic acid secretion can improve plant growth potential in abiotic stress: An example of Paecilomyces formosus LHL10 http://www.medworm.com/index.php?rid=5594077&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F3 Conclusion: The results reveal that mutualistic interactions of phytohormones secreting endophytic fungi can ameliorate host plant growth and alleviate adverse effects of salt stress. Such fungal strain could be used for further field trials to improve agricultural productivity under saline conditions. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5594077 Thu, 12 Jan 2012 05:00:00 +0100 5594077 Effect of modeled reduced gravity conditions on bacterial morphology and physiology http://www.medworm.com/index.php?rid=5594076&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F4 Conclusions: Overall, our data provides novel information about E. coli and S. aureus membrane potential and integrity and suggest that bacteria are physiologically more active and a larger percentage are viable under MRG as compared to NG conditions. In addition, these results demonstrate that bacterial physiological responses to MRG conditions vary with growth medium and growth phase demonstrating that nutrient resources are a modulator of response. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5594076 Thu, 12 Jan 2012 05:00:00 +0100 5594076 Bacillus anthracis-derived edema toxin (ET) counter-regulates movement of neutrophils and macromolecules through the endothelial paracellular pathway http://www.medworm.com/index.php?rid=5577124&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F2 Conclusions: We conclude that ET decreases IL-8-driven TEM of PMNs across HMVEC-L monolayers independent of cAMP/PKA activity. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5577124 Mon, 09 Jan 2012 05:00:00 +0100 5577124 Molecular analysis of methanogenic archaea in the forestomach of the alpaca (Vicugna pacos) http://www.medworm.com/index.php?rid=5569749&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F12%2F1 Conclusion: From our analysis, we propose a model to describe the population structure of Methanobrevibacter-related methanogens in the alpaca and in previously reported host species, which may contribute in unraveling the complexity of symbiotic archaeal communities in herbivores. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5569749 Thu, 05 Jan 2012 05:00:00 +0100 5569749 Genesis of a novel Shigella flexneri serotype by sequential infection of serotype-converting bacteriophages SfX and SfI http://www.medworm.com/index.php?rid=5556997&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F269 Conclusions: These findings suggest a new S. flexneri serotype could be created in nature. Such a conversion may be constrained by susceptibility of a strain to infection by a given serotype-converting bacteriophage. This finding has significant implications in the emergence of new S. flexneri serotypes in nature. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5556997 Fri, 30 Dec 2011 05:00:00 +0100 5556997 The promoter of filamentation (POF1) protein from Saccharomyces cerevisiae is an ATPase involved in the protein quality control process. http://www.medworm.com/index.php?rid=5550305&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F268 Conclusions: Taken together, the results strongly suggest that the biological function of Pof1p is related to the regulation of protein degradation. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5550305 Wed, 28 Dec 2011 05:00:00 +0100 5550305 Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS http://www.medworm.com/index.php?rid=5534295&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F267 Conclusions: MALDI-TOF-MS can be developed into a fast and reliable identification method for genetically highly related species when potential taxonomic and genetic inconsistencies are taken into consideration during the generation of the reference library. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5534295 Fri, 23 Dec 2011 05:00:00 +0100 5534295 Transformation of Botrytis cinerea by direct hyphal blasting or by wound-mediated transformation of sclerotia http://www.medworm.com/index.php?rid=5534296&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F266 Conclusions: These transformation methods, which are fast, simple and reproducible, can expedite functional gene analysis of B. cinerea. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5534296 Wed, 21 Dec 2011 05:00:00 +0100 5534296 Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1 http://www.medworm.com/index.php?rid=5520961&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F265 Conclusion: AggL is a new protein belonging to the collagen-binding superfamily of proteins and is sufficient for cell aggregation in lactococci. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5520961 Mon, 19 Dec 2011 05:00:00 +0100 5520961 Protective effect of probiotics on Salmonella infectivity assessed with combined in vitro gut fermentation-cellular models http://www.medworm.com/index.php?rid=5513026&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F264 Conclusions: Our results highlight the benefits of combining suitable cellular and colonic fermentation models to assess strain-specific first-level host protection properties of probiotics during Salmonella infection, providing an efficient system biology tool for preclinical development of new antimicrobials. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5513026 Thu, 15 Dec 2011 05:00:00 +0100 5513026 The streptococcal collagen-like protein-1 (Scl1) is a significant determinant for biofilm formation by group A Streptococcus http://www.medworm.com/index.php?rid=5501818&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F262 Conclusions: Overall, this work (i) identifies variations in biofilm formation capacity among pathogenically different GAS strains, (ii) identifies GAS surface properties that may aid in biofilm stability and, (iii) establishes that the Scl1 surface protein is an important determinant of GAS biofilm, which is sufficient to enable biofilm formation in the heterologous host Lactococcus. In summary, the GAS surface adhesin Scl1 may have an important role in biofilm-associated pathogenicity. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5501818 Wed, 14 Dec 2011 05:00:00 +0100 5501818 Impact of sub-inhibitory antibiotics on fibronectin-mediated host cell adhesion and invasion by Staphylococcus aureus http://www.medworm.com/index.php?rid=5501817&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F263 Conclusion: Our findings demonstrate that several antibiotics at sub-MICs modulate fibronectin binding in S. aureus in a drug-specific fashion. However, hyper- and hypo- adhesive phenotypes observed in controlled in vitro conditions were not fully confirmed in whole cell infection assays. The relevance of adhesion modulation during in vivo infections is thus still uncertain and requires further investigations. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5501817 Wed, 14 Dec 2011 05:00:00 +0100 5501817 Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR http://www.medworm.com/index.php?rid=5501819&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F261 Conclusions: Perturbation of the global regulator FNR and PurR functions as well as increase in purine nucleotide availability could affect the oxidative damage cell death pathway initiated by topoisomerase cleavage complex. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5501819 Mon, 12 Dec 2011 05:00:00 +0100 5501819 Cloning and characterization of a pectin lyase gene from Colletotrichum lindemuthianum and comparative phylogenetic/structural analyses with genes from phytopathogenic and saprophytic/opportunistic microorganisms http://www.medworm.com/index.php?rid=5493037&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F260 Conclusions: The Clpnl2 gene of C. lindemuthianum shares the characteristic elements of genes coding for pectin lyases. A time-course analysis revealed significant differences between the two fungal races in terms of the expression of Clpnl2 encoding for pectin lyase 2. According to the results, pectin lyases from bacteria and fungi separated early during evolution. Likewise, the enzymes from fungi and oomycetes diverged in accordance with their differing lifestyles. It is possible that the diversity and nature of the assimilatory carbon substrates processed by these organisms played a determinant role in this phenomenon. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5493037 Fri, 09 Dec 2011 05:00:00 +0100 5493037 The small heat shock proteins from Acidithiobacillus ferrooxidans: gene expression, phylogenetic analysis and structural modeling http://www.medworm.com/index.php?rid=5483945&cid=s_34035_77_f&fid=34035&url=%24%7Bitem.link%7D Conclusion: We conclude that the sHSPs encoded by the Afe_1437 and Afe_1009 genes are more likely to act as molecular chaperones in the A. ferrooxidans heat shock response. In addition, the three sHSPs from A. ferrooxidans are not recent paralogs, and the Afe_1437 and Afe_1009 genes could be inherited horizontally by A. ferrooxidans. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5483945 Wed, 07 Dec 2011 05:00:00 +0100 5483945 Contribution of bacterial outer membrane vesicles to innate bacterial defense http://www.medworm.com/index.php?rid=5465795&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F258 Conclusions: These data reveal a role for OMVs in contributing to innate bacterial defense by adsorption of antimicrobial peptides and bacteriophage. Given the increase in vesiculation in response to the antimicrobial peptides, and loss in efficiency of infection with the T4-OMV complex, we conclude that OMV production may be an important factor in neutralizing environmental agents that target the outer membrane of Gram-negative bacteria. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5465795 Thu, 01 Dec 2011 05:00:00 +0100 5465795 The alkylation response protein AidB is localized at the new poles and constriction sites in Brucella abortus http://www.medworm.com/index.php?rid=5437782&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F257 Conclusions: Data reported here suggest that AidB is a marker of new poles and constriction sites, that could be considered as sites of preparation of new poles in the sibling cells originating from cell division. The possible role of AidB in the generation or the function of new poles needs further investigation. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5437782 Wed, 23 Nov 2011 05:00:00 +0100 5437782 MLVA genotyping of Chinese human Brucella melitensis biovar 1, 2 and 3 isolates http://www.medworm.com/index.php?rid=5437783&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F256 Conclusion: The MLVA-16 assay can be useful to reveal the predominant genotypes and strain relatedness in endemic or non-endemic regions of brucellosis. However it is not suitable for biovar differentiation of B. melitensis. Genotype 42 is widely distributed throughout China during a long time. Bruce 16 and bruce 30 in panel 2B markers are most useful for typing Chinese isolates. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5437783 Tue, 22 Nov 2011 05:00:00 +0100 5437783 Detection of Legionella by quantitative-Polymerase Chain Reaction (qPCR) for monitoring and risk assessment http://www.medworm.com/index.php?rid=5437785&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F254 Conclusion: Detection by qPCR was suitable for monitoring changes in the concentration of Legionella but the precise determination of bacteria is difficult. Risk assessment by qPCR only on samples without any background information regarding treatment, timing, etc is dubious. However, the rapid detection by qPCR of high concentrations of Legionella - especially Legionella pneumophila - is valuable as an indicator of risk, although it may be false positive compared to culture results. On the other hand, the detection of a low number of bacteria by qPCR is a strong indication for the absence of risk. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5437785 Mon, 21 Nov 2011 05:00:00 +0100 5437785 Life without a Cell Membrane: Challenging the Specificity of Bacterial Endophytes within Bryopsis (Bryopsidales, Chlorophyta) http://www.medworm.com/index.php?rid=5437784&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F255 Conclusions: This suggests that Bryopsis algae seem to master transient stages of life without a cell membrane well as they harbor specific - and possibly ecological significant - endophytic bacteria. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5437784 Mon, 21 Nov 2011 05:00:00 +0100 5437784 Integration of a laterally acquired gene into a cell network important for growth in a strain of Vibrio rotiferianus http://www.medworm.com/index.php?rid=5418593&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F253 Conclusions: Mobile genes that are highly strain specific are generally believed to act in isolation. This is because perturbation of existing cell machinery by the acquisition of a new gene by LGT is highly likely to lower fitness. In contrast, we show here that at least one mobile gene, apparently unique to a strain, encodes a product that has integrated into central cellular metabolic processes such that it greatly lowers fitness when lost under those conditions likely to be commonly encountered for the free living cell. This has ramifications for our understanding of the role mobile gene encoded products play in the cell from a systems biology perspective. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5418593 Fri, 18 Nov 2011 05:00:00 +0100 5418593 The bacteriophage WORiC is the active phage element in wRi of Drosophila simulans and represents a conserved class of WO phages http://www.medworm.com/index.php?rid=5418595&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F251 Conclusions: The results presented here suggest that WORiC is the lytic form of WO in D. simulans, is undergoing extrachromosomal replication in wRi, and belongs to a conserved family of phages in Wolbachia. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5418595 Tue, 15 Nov 2011 05:00:00 +0100 5418595 An alternative physiological role for the EmhABC efflux pump in Pseudomonas fluorescens cLP6a http://www.medworm.com/index.php?rid=5418594&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F252 Conclusions: Combined, these observations suggest that the EmhABC efflux pump may be involved in the management of membrane stress effects such as those due to unfavourable incubation temperatures. Efflux of fatty acids replaced as a result of membrane damage or phospholipid turnover may be the primary physiological role of the EmhABC efflux pump in P. fluorescens cLP6a. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5418594 Tue, 15 Nov 2011 05:00:00 +0100 5418594 Transcriptome and membrane fatty acid analyses reveal different strategies for responding to permeating and non-permeating solutes in the bacterium Sphingomonas wittichii http://www.medworm.com/index.php?rid=5404971&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F250 Conclusions: A combination of growth assays, transcriptome profiling, and membrane fatty acid analyses revealed that permeating and non-permeating solutes trigger different adaptive responses in strain RW1, suggesting these solutes affect cells in fundamentally different ways. Future work is now needed that connects these responses with the responses observed in more realistic scenarios of soil desiccation. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5404971 Mon, 14 Nov 2011 05:00:00 +0100 5404971 Proteome driven re-evaluation and functional annotation of the Streptococcus pyogenes SF370 genome. http://www.medworm.com/index.php?rid=5397565&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F249 Conclusions: The list of amino acid sequences that were annotated by genome analysis contains outdated information and unrecognized protein-coding sequences. We suggest that the six-frame database derived from actual DNA sequences be used for reliable proteomic analysis. In addition, the experimental evidence from functional proteomic analysis is useful for the re-evaluation of previously sequenced genomes. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5397565 Thu, 10 Nov 2011 05:00:00 +0100 5397565 The uncertain consequences of transferring bacterial strains between laboratories - rpoS instability as an example http://www.medworm.com/index.php?rid=5397566&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F248 Conclusions: Given that many E. coli strains contain high RpoS levels similar to MC4100TF, the integrity of such strains during transfers and storage is questionable. Variations in important collections may be due to storage-transfer related issues. These results raise important questions on the integrity of bacterial archives and transferred strains, explain variation like in the ECOR collection between laboratories and indicate a need for the development of better methods of strain transfer. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5397566 Tue, 08 Nov 2011 05:00:00 +0100 5397566 Oral Candida albicans isolates from HIV-positive individuals have similar in vitro biofilm-forming ability and pathogenicity as invasive Candida isolates http://www.medworm.com/index.php?rid=5376624&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F247 Conclusions: We found that on silicone pads as well as in the Galleria model, biofilm formation and virulence depends on the Candida species. Importantly, for C. albicans the pathogenicity of oral Candida isolates was similar to systemic Candida isolates, suggesting that Candida isolates have similar biofilm-forming ability and virulence regardless of the infection site from which it was isolated. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5376624 Fri, 04 Nov 2011 04:00:00 +0100 5376624 Comparing Enterovirus 71 with Coxsackievirus A16 by analyzing nucleotide sequences and antigenicity of recombinant proteins of VP1s and VP4s http://www.medworm.com/index.php?rid=5376625&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F246 Background: Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) are two major etiological agents of Hand, Foot and Mouth Disease (HFMD). EV71 is associated with severe cases but not CA16. The mechanisms contributed to the different pathogenesis of these two viruses are unknown. VP1 and VP4 are two major structural proteins of these viruses, and should be paid close attention to. Results: The sequences of vp1s from 14 EV71 and 14 CA16, and vp4s from 10 EV71 and 1 CA16 isolated in this study during 2007 to 2009 HFMD seasons were analyzed together with the corresponding sequences available in GenBank using DNAStar and MEGA 4.0. Phylogenetic analysis of complete vp1s or vp4s showed that EV71 isolated in Beijing belonged to C4 and CA16 belonged to lineage B2 (lineage C). VP1s and VP4s from 4 st... BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5376625 Thu, 03 Nov 2011 04:00:00 +0100 5376625 Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons http://www.medworm.com/index.php?rid=5376627&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F244 Background: Urine within the urinary tract is commonly regarded as "sterile" in cultivation terms. Here, we present a comprehensive in-depth study of bacterial 16S rDNA sequences associated with urine from healthy females by means of culture-independent high-throughput sequencing techniques. Results: Sequencing of the V1V2 and V6 regions of the 16S ribosomal RNA gene using the 454 GS FLX system was performed to characterize the possible bacterial composition in 8 culture-negative ( (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5376627 Wed, 02 Nov 2011 04:00:00 +0100 5376627 Biofilm and planktonic pneumococci demonstrate disparate immunoreactivity to human convalescent sera http://www.medworm.com/index.php?rid=5376626&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F245 Conclusion: Differential protein production by planktonic and biofilm pneumococci provides a potential explanation for why individuals remain susceptible to invasive disease despite previous colonization events. These findings also strongly suggest that differential protein production during colonization and disease be considered during the selection of antigens for any future protein vaccine. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5376626 Wed, 02 Nov 2011 04:00:00 +0100 5376626 Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters http://www.medworm.com/index.php?rid=5356049&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F243 Conclusions: We have developed a gene cloning and expression system in a fast-growing and moderately thermophilic Streptomyces species. Although just a few plasmids and one antibiotic biosynthetic gene cluster from mesophilic Streptomyces were successfully expressed in thermophilic Streptomyces species, we expect that by utilizing thermophilic Streptomyces-specific promoters, more genes and especially antibiotic genes clusters of mesophilic Streptomyces should be heterologously expressed. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5356049 Fri, 28 Oct 2011 04:00:00 +0100 5356049 Activation of PI3K/AKT and ERK MAPK signal pathways is required for the induction of lytic cycle replication of Kaposi's Sarcoma-associated herpesvirus by herpes simplex virus type 1 http://www.medworm.com/index.php?rid=5356052&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F240 Conclusions: HSV-1 infection stimulated PI3K/AKT and ERK MAPK signaling pathways that in turn contributed to KSHV reactivation, which provided further insights into the molecular mechanism controlling KSHV lytic replication, particularly in the context of HSV-1 and KSHV co-infection. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5356052 Thu, 27 Oct 2011 04:00:00 +0100 5356052 Exploring the contribution of efflux on the resistance to fluoroquinolones in clinical isolates of Staphylococcus aureus http://www.medworm.com/index.php?rid=5356051&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F241 Conclusions: The results obtained in this work do not exclude the importance of mutations in resistance to fluoroquinolones in S. aureus, yet they underline the contribution of efflux systems for the emergence of high-level resistance. All together, the results presented in this study show the potential role played by efflux systems in the development of resistance to fluoroquinolones in clinical isolates of S. aureus. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5356051 Thu, 27 Oct 2011 04:00:00 +0100 5356051 Niche differentiation of two sympatric species of Microdochium colonizing the roots of common reed http://www.medworm.com/index.php?rid=5356050&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F242 Conclusions: The fungal species colonizing common reed investigated in this study seem to exploit niche differences leading to a separation in space and time, which may allow for their coexistence on the same host. A purely neutral model is unlikely to explain the coexistence of closely related endophytes on common reed. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5356050 Thu, 27 Oct 2011 04:00:00 +0100 5356050 Arcanolysin is a cholesterol-dependent cytolysin of the human pathogen Arcanobacterium haemolyticum http://www.medworm.com/index.php?rid=5356053&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F239 Conclusions: ALN is a newly identified CDC with hemolytic activity and unique properties in the CDC family and may be a virulence determinant for A. haemolyticum. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5356053 Wed, 26 Oct 2011 04:00:00 +0100 5356053 Differential regulation of two closely related integrative and conjugative elements from Streptococcus thermophilus. http://www.medworm.com/index.php?rid=5356054&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F238 Conclusions: All together, these results suggest a novel mechanism of regulation shared by ICESt1, ICESt3 and closely related ICEs, which we identified by analysis of recently sequenced genomes of firmicutes. This is the first report of a partial shutdown of the activity of an ICE executed by a strain belonging to its primary host species. The sharp increase of ICESt3 copy number suggests an induction of replication; such conditional intracellular replication may be common among ICEs. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5356054 Mon, 24 Oct 2011 04:00:00 +0100 5356054 Mycobacterium tuberculosis infection induces non-apoptotic cell death of human dendritic cells http://www.medworm.com/index.php?rid=5344044&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F237 Conclusions: Human DCs undergo cell death after infection with live Mtb, in a manner that does not involve executioner caspases, and results in no mycobactericidal effect. Nonetheless, the DC maturation and cytokine profile observed suggests that the infected cells can still contribute to TB immunity. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5344044 Mon, 24 Oct 2011 04:00:00 +0100 5344044 Molecular profiling of fungal communities in moisture damaged buildings before and after remediation - a comparison of culture-dependent and culture-independent methods http://www.medworm.com/index.php?rid=5344046&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F235 Conclusions: We examined a small number of target buildings and found indications of elevated fungal diversity associated with water damage. Some of the fungi in dust were attributable to building growth, but more information on the material-associated communities is needed in order to understand the dynamics of microbial communities between building structures and dust. The sequencing-based method proved indispensable for describing the true fungal diversity in indoor environments. However, making conclusions concerning the effect of building conditions on building mycobiota using this methodology was complicated by the wide natural diversity in the dust samples, the incomplete knowledge of material-associated fungi and the semiquantitative nature of sequencing based methods. (Source: BMC... BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5344046 Fri, 21 Oct 2011 04:00:00 +0100 5344046 The Fur Regulon in Anaerobically Grown Salmonella enterica sv. Typhimurium: Identification of New Fur Targets http://www.medworm.com/index.php?rid=5344045&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F236 Conclusions: This study determined that Fur is a positive factor in ftnB regulation, while serving to repress the expression of hmpA. Furthermore, Fur is required for the proper expression and activation of the antioxidant enzymes, FeSOD and MnSOD. Finally, this work identified twenty-six new targets of Fur regulation, and demonstrates that H-NS repressed genes are down-regulated in [increment]fur. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5344045 Fri, 21 Oct 2011 04:00:00 +0100 5344045 Flavivirus NS3 and NS5 proteins interaction network: A high-throughput yeast two-hybrid screen. http://www.medworm.com/index.php?rid=5344047&cid=s_34035_77_f&fid=34035&url=%24%7Bitem.link%7D Conclusions: We proposed that the selective disruption of these newly identified host/virus interactions could represent a novel and attractive therapeutic strategy in treating flavivirus infections. Our virus-host interaction map provides a basis to unravel fundamental processes about flavivirus subversion of the host replication machinery and/or immune defence strategy. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5344047 Thu, 20 Oct 2011 04:00:00 +0100 5344047 Immunodominant proteins alpha-1 giardin and beta-giardin are expressed in both assemblages A and B of Giardia lamblia http://www.medworm.com/index.php?rid=5331397&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F233 Conclusions: We found differences in localization of the beta-giardin protein between assemblages A and B, but the same pattern of localization of alpha-1 giardin in strains from Assemblages A, B and E. These findings reinforce the need for more studies based on phenotypic characteristics in order to disclose how far one assemblage is from the other. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5331397 Wed, 19 Oct 2011 04:00:00 +0100 5331397 Characterization of a Mycobacterium smegmatis uvrA mutant impaired in dormancy induced by hypoxia and low carbon concentration http://www.medworm.com/index.php?rid=5331399&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F231 Conclusions: Taken together our results confirm that the mycobacterial NER system is involved in adaptation to various stress conditions and suggest that cells with a compromised DNA repair system have an impaired dormancy behavior. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5331399 Tue, 18 Oct 2011 04:00:00 +0100 5331399 A Historical and Proteomic Analysis of Botulinum Neurotoxin Type /G http://www.medworm.com/index.php?rid=5331398&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F232 Conclusions: The in silico protein sequence comparisons established that the /G complex is phenetically related to the other six serotypes of C. botulinum. Proteomic analyses and Endopep-MS confirmed the presence of BoNT and NAPs, along with the activity of the commercial /G complex. The use of data-independent MSE data analysis, coupled to label-free quantification software, suggested that the weight ratio BoNT:NAPs is 1:3, whereas the molar ratio of BoNT:NTNH:HA70:HA17 is 1:1:2:1, within the BoNT /G progenitor toxin. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5331398 Tue, 18 Oct 2011 04:00:00 +0100 5331398 Identification and characterization of PhbF: a DNA binding protein with regulatory role in the PHB metabolism of Herbaspirillum seropedicae SmR1. http://www.medworm.com/index.php?rid=5321965&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F230 Conclusions: Our results indicate that H. seropedicae SmR1 PhbF regulates expression of phb-related genes by acting as a transcriptional repressor. The knowledge of the PHB metabolism of this plant-associated bacterium may contribute to the understanding of the plant-colonizing process and the organism's resistance and survival in planta. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5321965 Fri, 14 Oct 2011 04:00:00 +0100 5321965 Genetic relationships among Italian and Mexican maize-rhizosphere Burkholderia cepacia complex (BCC) populations belonging to Burkholderia cenocepacia IIIB and BCC6 group http://www.medworm.com/index.php?rid=5311615&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F228 Conclusions: Taken together our data demonstrate a wide dispersal of certain B. cenocepacia IIIB and BCC6 isolates in Mexican and Italian maize rhizospheres. Despite the clear relationship found between the geographic origin of isolates and grouping, identical RTs and closely related isolates were observed in geographically distant regions. Ecological factors and selective pressure may preferably promote some genotypes within each local microbial population, favouring the spread of a single clone above the rest of the recombinant population. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5311615 Thu, 13 Oct 2011 04:00:00 +0100 5311615 Regulation of phenylacetic acid uptake is sigma54 dependent in Pseudomonas putida CA-3. http://www.medworm.com/index.php?rid=5311614&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F229 Conclusions: In summary, this is the first study to report RpoN dependent transcriptional activation of the PACoA catabolon paaL gene, encoding a transport protein essential for phenylacetic acid utilisation in P. putida CA-3. Bioinformatic analysis is provided to suggest this regulatory link may be common among styrene degrading Pseudomonads. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5311614 Thu, 13 Oct 2011 04:00:00 +0100 5311614 Na+-stimulated phosphate uptake system in Synechocystis sp. PCC 6803 with Pst1 as a main transporter http://www.medworm.com/index.php?rid=5311618&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F225 Conclusion: The data demonstrate that Pst2 system of Synechocystis has higher affinity toward phosphate with lower Vmax than Pst1 system. The Pst1 system had similar Km and Vmax values to those of the wild type suggesting that Pst1 is the main phosphate transporter in Synechocystis sp. PCC 6803. The Km of Pst1 of Synechocystis is closer to that of Pit system than to that of the Pst system of E. coli, suggesting that Synechocystis Pst1 is rather a medium/low affinity transporter whereas Pst2 is a high affinity transporter. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5311618 Tue, 11 Oct 2011 04:00:00 +0100 5311618 A novel bacteriophage Tail-Associated Muralytic Enzyme (TAME) from Phage K and its development into a potent antistaphylococcal protein http://www.medworm.com/index.php?rid=5311617&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F226 Conclusions: We identified a phage K gene that encodes a protein associated with the phage tail structure. The muralytic activity of the phage K TAME was localized to the C-terminal CHAP domain. This potent antistaphylococcal TAME was combined with an efficient Staphylococcus-specific cell-wall targeting domain SH3b, resulting in the chimeric protein P128. This protein shows bactericidal activity against globally prevalent antibiotic resistant clinical isolates of S. aureus and against the genus Staphylococcus in general. In vivo, P128 was efficacious against methicillin-resistant S. aureus in a rat nasal colonization model. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5311617 Tue, 11 Oct 2011 04:00:00 +0100 5311617 CcpA represses the expression of the divergent cit operons of Enterococcus faecalis through multiple cre sites. http://www.medworm.com/index.php?rid=5311616&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F227 Conclusion: In conclusion, our study establishes that expression of the cit operons in E. faecalis is controlled by CCR via CcpA-dependent and -independent mechanisms. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5311616 Tue, 11 Oct 2011 04:00:00 +0100 5311616 Genome organization of epidemic Acinetobacter baumannii strains. http://www.medworm.com/index.php?rid=5302094&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F224 Conclusion: The definition of the genome components of A. baumannii provides a scaffold to rapidly evaluate the genomic organization of novel clinical A. baumannii isolates. Changes in island profiling will be useful in genomic epidemiology of A. baumannii population. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5302094 Mon, 10 Oct 2011 04:00:00 +0100 5302094 The rgg0182 gene encodes a transcriptional regulator required for the full Streptococcus thermophilus LMG18311 thermal adaptation. http://www.medworm.com/index.php?rid=5294080&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F223 Conclusions: These data showed the importance of the Rgg0182 transcriptional regulator on the survival of S. thermophilus during dairy processes and more specifically during changes in temperature. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5294080 Fri, 07 Oct 2011 04:00:00 +0100 5294080 Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization http://www.medworm.com/index.php?rid=5282112&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F219 Conclusion: As shown by the parallel microbiology and metabolome approach, the gluten-free diet lasting at least two years did not completely restore the microbiota and, consequently, the metabolome of CD children. Some molecules (e.g., ethyl-acetate and octyl-acetate, some short chain fatty acids and free amino acids, and glutamine) seems to be metabolic signatures of CD. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5282112 Tue, 04 Oct 2011 04:00:00 +0100 5282112 Development of an indirect competitive enzyme-linked immunosorbent assay applied to the Botrytis cinerea quantification in tissues of postharvest fruits. http://www.medworm.com/index.php?rid=5282111&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F220 Conclusions: The developed method allowed detects the presence of B. cinerea in asymptomatic fruits and provides the advantages of low cost, easy operation, and short analysis time determination for its possible application in the phytosanitary programs of the fruit industry worldwide. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5282111 Tue, 04 Oct 2011 04:00:00 +0100 5282111 A metagenomic study of methanotrophic microorganisms in Coal Oil Point seep sediments http://www.medworm.com/index.php?rid=5282110&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F221 Conclusions: Most of the taxa detected were present in both metagenomes and differences in community structure and corresponding metabolic potential between the two samples were mainly due to abundance differences.The results suggests that the Tonya Seep sediment is a robust methane filter, where taxa presently dominating this process could be replaced by less abundant methanotrophic taxa in case of changed environmental conditions. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5282110 Tue, 04 Oct 2011 04:00:00 +0100 5282110 Characterization of pyoverdine and achromobactin in Pseudomonas syringae pv. phaseolicola 1448a http://www.medworm.com/index.php?rid=5282113&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F218 Conclusions: This study provides the first evidence of a P. syringae pathovar producing a side chain variant form of pyoverdine. We also describe novel IC50 and liquid CAS assays to quantify the contribution of different siderophores across a range of iron starvation conditions, and show that although achromobactin has potential to contribute to fitness its contribution is masked by the presence of pyoverdine, which is a significantly more effective siderophore. Neither pyoverdine nor achromobactin appear to be required for P. syringae 1448a to cause bean halo blight, indicating that these siderophores are not promising targets for crop protection strategies. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5282113 Mon, 03 Oct 2011 04:00:00 +0100 5282113 Comparison of PCR Ribotyping and Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) for Improved Detection of Clostridium difficile http://www.medworm.com/index.php?rid=5269808&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F217 Conclusions: For the epidemiological investigations of C. difficile, we recommend that MLVA10 be used in coordination with the PCR-ribotype groups to detect epidemic clones, and that the MLVA4 could be used to detect outbreak strains. MLVA10 and MLVA4 could be combined in four multiplex PCR reactions to save time and obtain distinguishable data. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5269808 Fri, 30 Sep 2011 04:00:00 +0100 5269808 Evolution and diversity of community-associated methicillin-resistant Staphylococcus aureus in a geographical region http://www.medworm.com/index.php?rid=5269810&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F215 Conclusions: Although multiple CA-MRSA clones have evolved in the Western Australian community only three clones have successfully adapted to the Western Australian community environment. These data suggest the successful evolution of a CA-MRSA clone may not only depend on the mobility of the SCCmec element but also on other genetic determinants. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5269810 Thu, 29 Sep 2011 04:00:00 +0100 5269810 Experimental annotation of the human pathogen Histoplasma capsulatum transcribed regions using high-resolution tiling arrays http://www.medworm.com/index.php?rid=5269809&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F216 Conclusions: These analyses yielded a high confidence set of validated gene predictions for H. capsulatum. The transcript sets resulting from this study are a valuable resource for further experimental characterization of this ubiquitous fungal pathogen. The data is available for interactive exploration at http://histo.ucsf.edu. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5269809 Thu, 29 Sep 2011 04:00:00 +0100 5269809 The role of Candida albicans homologous recombination factors Rad54 and Rdh54 in DNA damage sensitivity http://www.medworm.com/index.php?rid=5269811&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F214 Conclusions: Together, these results support a role for homologous recombination in genome stability under nondamaging conditions. The nuclear morphology defects in the rad54Delta/rad54Delta mutants show that Rad54 performs an essential role during mitotic growth and that in its absence, cells arrest in G2. The viability of the single mutant rad54Delta/rad54Delta and the inability to construct the double mutant rad54Delta/rad54Delta rdh54Delta/rdh54Delta suggests that Rdh54 can partially compensate for Rad54 during mitotic growth. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5269811 Tue, 27 Sep 2011 04:00:00 +0100 5269811 Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage http://www.medworm.com/index.php?rid=5257738&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F211 Conclusions: Our results suggest that gp9 at the phage tip is suitable for the phage display technology. The modified gp9 can be supplied in trans from a plasmid and fully complements M13 phage with an amber mutation in gene 9. The modified phage tip is very well accessible to antibodies. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5257738 Mon, 26 Sep 2011 04:00:00 +0100 5257738 Prevention of siderophore- mediated gut-derived sepsis due to P. aeruginosa can be achieved without iron provision by maintaining local phosphate abundance: role of pH http://www.medworm.com/index.php?rid=5257737&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F212 In this study we examined the role of pH in the protective effect of [Pi] supplementation as it has been shown to be increased in the distal gut following surgical injury. Surgically injured mice drinking 25 mM [Pi] at pH 7.5 and intestinally inoculated with P. aeruginosa had increased mortality compared to mice drinking 25 mM [Pi] at pH 6.0 (p (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5257737 Mon, 26 Sep 2011 04:00:00 +0100 5257737 The protein interaction map of bacteriophage lambda http://www.medworm.com/index.php?rid=5257736&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F213 Conclusions: Phage lambda serves as a benchmark for future studies of protein interactions among phage, viruses in general, or large protein assemblies. We conclude that we could not find all the known interactions because they require chaperones, post-translational modifications, or multiple proteins for their interactions. The lambda protein network connects 12 proteins of unknown function with well characterized proteins, which should shed light on the functional associations of these uncharacterized proteins. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5257736 Mon, 26 Sep 2011 04:00:00 +0100 5257736 Mobilisation and remobilisation of a large archetypal pathogenicity island of uropathogenic Escherichia coli in vitro support the important role of conjugation for horizontal transfer of genomic islands http://www.medworm.com/index.php?rid=5257739&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F210 Conclusions: Our results corroborate that mobilisation and conjugal transfer may contribute to evolution of bacterial pathogens through horizontal transfer of large chromosomal regions such as PAIs. Stabilisation of these mobile genetic elements in the bacterial chromosome may result from selective loss of mobilisation and transfer functions of genomic islands. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5257739 Sat, 24 Sep 2011 04:00:00 +0100 5257739 The Aspergillus giganteus antifungal protein AFPNN5353 activates the cell wall integrity pathway and perturbs calcium homeostasis http://www.medworm.com/index.php?rid=5246948&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F209 Conclusions: The present study contributes new insights into the molecular mechanisms of action of the A. giganteus antifungal protein AFPNN5353. We identified its antifungal activity, initiated the investigation of pathways that determine protein toxicity, namely the CWIP and the Ca2+ signalling cascade, and studied in detail the cellular uptake mechanism in sensitive target fungi. This knowledge contributes to define new potential targets for the development of novel antifungal strategies to prevent and combat infections of filamentous fungi which have severe negative impact in medicine and agriculture. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5246948 Fri, 23 Sep 2011 04:00:00 +0100 5246948 Roles of RpoN in the Resistance of Campylobacter jejuni under Various Stress Conditions http://www.medworm.com/index.php?rid=5246950&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F207 Conclusions: The results demonstrate that the RpoN sigma factor plays a dynamic role in C. jejuni defense against various stresses which this bacterial pathogen may encounter during transmission to and infection of humans. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5246950 Thu, 22 Sep 2011 04:00:00 +0100 5246950 Expression of the Flp Proteins by Haemophilus ducreyi is necessary for virulence in human volunteers http://www.medworm.com/index.php?rid=5246949&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F208 Conclusion: These data suggest that production and secretion of the Flp proteins contributes to microcolony formation and attachment to HFF cells in vitro. Expression of flp1-3 is also necessary for H. ducreyi to initiate disease and progress to pustule formation in humans. Future studies will focus on how Flp proteins contribute to microcolony formation and attachment in vivo. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5246949 Thu, 22 Sep 2011 04:00:00 +0100 5246949 In Silico Identification of Pathogenic Strains of Cronobacter from Biochemical Data Reveals Association of Inositol Fermentation with Pathogenicity http://www.medworm.com/index.php?rid=5246953&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F204 Conclusions: We demonstrated a computational approach allowing existing diagnostic kits to be used to identify pathogenic strains of Cronobacter. The resulting clusters correlated well with MLST sequence types and revealed new information about the pathogenicity of Cronobacter species. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5246953 Tue, 20 Sep 2011 04:00:00 +0100 5246953 Role of EscU auto-cleavage in promoting type III effector translocation into host cells by Enteropathogenic Escherichia coli http://www.medworm.com/index.php?rid=5246952&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F205 Conclusions: These data provide new insight into the role of EscU auto-cleavage in EPEC. The experimental data suggests that EscU auto-cleavage results in a suitable binding interface at the inner membrane that accommodates protein complexes during type III secretion events. The results also demonstrate that altered EPEC genetic backgrounds that display intermediate levels of effector secretion and translocation can be isolated and studied. These genetic backgrounds should be valuable in deciphering sequential and temporal events involved in EPEC type III secretion. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5246952 Tue, 20 Sep 2011 04:00:00 +0100 5246952 Clonal Diversity of the Glutamate Dehydrogenase Gene in Giardia duodenalis from Thai Isolates: Evidence of Genetic Exchange or Mixed Infections? http://www.medworm.com/index.php?rid=5246951&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F206 Conclusion: This study supports increasing evidence that G. duodenalis has the potential for genetic exchange. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5246951 Tue, 20 Sep 2011 04:00:00 +0100 5246951 A new class of IMP dehydrogenase with a role in self-resistance of mycophenolic acid producing fungi http://www.medworm.com/index.php?rid=5234316&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F202 Conclusions: We identified a novel variant of the IMPDH-encoding gene in six different strains from Penicillium subgenus Penicillium. The novel IMPDH variant from MPA producer P. brevicompactum was shown to confer a high degree of MPA resistance when expressed in a non-producer fungus. Our study provides a basis for understanding the molecular mechanism of MPA resistance and has relevance for biotechnological and pharmaceutical applications. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5234316 Fri, 16 Sep 2011 04:00:00 +0100 5234316 Global population structure of Aspergillus terreus inferred by ISSR typing reveals geographical subclustering http://www.medworm.com/index.php?rid=5234315&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F203 Conclusions: This study provides evidence of a population structure linked to geographical origin in A. terreus. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5234315 Fri, 16 Sep 2011 04:00:00 +0100 5234315 SNP diversity of Enterococcus faecalis and Enterococcus faecium in a South East Queensland Waterway, Australia, and associated antibiotic resistance gene profiles http://www.medworm.com/index.php?rid=5218267&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F201 Conclusions: The distribution of E. faecalis and E. faecium genotypes is highly diverse in the Coomera River. The SNP genotyping method is rapid and robust and can be applied to study the diversity of E. faecalis and E. faecium in waterways. It can also be used to test for human-related and human-specific enterococci in water. The resolving power can be increased by including antibiotic-resistant profiles which can be used as a possible source tracking tool. This warrants further investigation. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5218267 Mon, 12 Sep 2011 04:00:00 +0100 5218267 Serotype and genotype analysis of Dengue Virus by Sequencing followed by Phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan http://www.medworm.com/index.php?rid=5204911&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F200 Conclusions: Our study showed that serotype DEN-2 was dominant in positive samples of dengue virus infection collected during the period of three years (2007-2009). The other serotype present was serotype DEN-3. Genotypes of serotype DEN-2 and serotype DEN-3 were subtype IV and subtype III, respectively. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5204911 Sat, 10 Sep 2011 04:00:00 +0100 5204911 Mutation of L-2,3-diaminopropionic acid synthase genes blocks staphyloferrin B biosynthesis in Staphylococcus aureus http://www.medworm.com/index.php?rid=5204912&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F199 Conclusions: Mutation of either sbnA or sbnB result in abrogation of synthesis of staphyloferrin B, a siderophore that contributes to iron-restricted growth of S. aureus. The loss of staphyloferrin B synthesis is due to an inability to synthesize the unusual amino acid L-2,3-diaminopropionic acid which is an important, iron-liganding component of the siderophore structure. It is proposed that SbnA and SbnB function together as an L-Dap synthase in the S. aureus cell. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5204912 Fri, 09 Sep 2011 04:00:00 +0100 5204912 Carbohydrate-active enzymes from pigmented Bacilli: a genomic approach to assess carbohydrate utilization and degradation http://www.medworm.com/index.php?rid=5194826&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F198 Conclusions: CAZy analyses of the genomes of the two pigmented Bacilli, compared to other Bacillus species and validated by experimental data on carbohydrate utilization, biofilm formation and mucin degradation, suggests that the two pigmented Bacilli are adapted to the intestinal environment and are suited to grow in and colonize the human gut. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5194826 Sun, 04 Sep 2011 23:00:00 +0100 5194826 Lysis-deficient phages as novel therapeutic agents for controlling bacterial infection http://www.medworm.com/index.php?rid=5191691&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F195 Conclusions: A recombinant endolysin-deficient staphylococcal phage has been developed that is lethal to methicillin-resistant S. aureus without causing bacterial cell lysis. The phage was able to multiply in lytic mode utilizing a heterologous endolysin expressed from a plasmid in the propagation host. The recombinant phage effectively rescued mice from fatal S. aureus infection. To our knowledge this is the first report of a lysis-deficient staphylococcal phage. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5191691 Tue, 30 Aug 2011 23:00:00 +0100 5191691 Iron Restriction Induces Preferential Down-Regulation of H2-Consuming over H2-Evolving Reactions During Fermentative Growth of Escherichia coli http://www.medworm.com/index.php?rid=5191690&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F196 Conclusions: Our findings demonstrate that the ferrous iron transport system is the principal route of iron uptake for anaerobic hydrogenase biosynthesis, with a contribution from the ferric-enterobactin system. Hydrogen-oxidizing enzyme function was abolished in a feoB entC double mutant and this appears to be due to post-translational effects. The retention of residual hydrogen-evolving activity, even in the feoB entC double null mutant suggests that sufficient iron can be scavenged to synthesize this key fermentative enzyme complex in preference to the hydrogen-uptake enzymes. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5191690 Tue, 30 Aug 2011 23:00:00 +0100 5191690 Genotypic and phenotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from clinical and environmental sources including High-purity Water. http://www.medworm.com/index.php?rid=5179374&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F194 Conclusions: R. pickettii species isolated from wide geographic and environmental sources appear to be reasonably homogenous based on genotypic and phenotypic characteristics. R. insidiosa can at present only be distinguished from R. pickettii using species specific PCR. R. pickettii and R. insidiosa isolates do not differ significantly phenotypically or genotypically based on environmental or geographical origin. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5179374 Mon, 29 Aug 2011 23:00:00 +0100 5179374 Evaluation of stool microbiota signatures in two cohorts of Asian (Singapore and Indonesia) newborns at risk of atopy http://www.medworm.com/index.php?rid=5169217&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F193 Conclusions: Differences in stool microbiota signatures were observed in relation to various demographic factors. These features may confound studies relating to the association of the structure of fecal microbiota and the predisposition to human modern disease. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5169217 Thu, 25 Aug 2011 23:00:00 +0100 5169217 A rapid in situ procedure for determination of bacterial susceptibility or resistance to antibiotics that inhibit peptidoglycan biosynthesis http://www.medworm.com/index.php?rid=5169219&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F191 Conclusion: The technique to assess cell wall integrity appears to be a rapid and simple procedure to identify resistant and susceptible strains to antibiotics that interfere with peptidoglycan biosynthesis. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5169219 Wed, 24 Aug 2011 23:00:00 +0100 5169219 Transcriptomes of Frankia sp. strain CcI3 in growth transitions http://www.medworm.com/index.php?rid=5169218&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F192 Conclusions: The overall pattern of gene expression in aging cultures of CcI3 suggests significant cell heterogeneity even during normal growth on ammonia. The detection of abundant transcription of nif (nitrogen fixation) genes likely reflects the presence of anaerobic, N-depleted microsites in the growing mycelium of the culture, and the presence of significantly elevated transposase transcription during starvation indicates the continuing evolution of the Frankia sp. strain CcI3 genome, even in culture, especially under stressed conditions. These studies also sound a cautionary note when comparing the transcriptomes of Frankia grown in root nodules, where cell heterogeneity would be expected to be quite high. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5169218 Wed, 24 Aug 2011 23:00:00 +0100 5169218 A bacterial PriB with weak single-stranded DNA binding activity can stimulate the DNA unwinding activity of its cognate PriA helicase http://www.medworm.com/index.php?rid=5156663&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F189 Conclusions: The results of this study demonstrate that a bacterial PriB homolog with weak single-stranded DNA binding activity can stimulate the DNA unwinding activity of its cognate PriA helicase. While it remains unclear if N. gonorrhoeae PriB's weak DNA binding activity is required for PriB stimulation of PriA helicase, the ability of PriB to stimulate PriA-catalyzed ATP hydrolysis could play an important role. Thus, the weak interaction between N. gonorrhoeae PriB and DNA might be compensated for by the strong interaction between PriB and PriA, which could result in allosteric activation of PriA's ATPase activity. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5156663 Mon, 22 Aug 2011 23:00:00 +0100 5156663 Glucose and ethanol-dependent transcriptional regulation of the astaxanthin biosynthesis pathway in Xanthophyllomyces dendrorhous. http://www.medworm.com/index.php?rid=5156662&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F190 Conclusion: For the first time, we demonstrated that carbon source-dependent regulation of astaxanthin biosynthesis in X. dendrorhous involves changes at the transcriptional level. Such a regulatory mechanism provides an explanation for the strong and early inhibitory effect of glucose on the biosynthesis of this carotenoid. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5156662 Mon, 22 Aug 2011 23:00:00 +0100 5156662 The influence of the Cage System and colonisation of Salmonella Enteritidis on the Microbial Gut Flora of laying Hens studied by T-RFLP and 454 pyrosequencing http://www.medworm.com/index.php?rid=5156665&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F187 Conclusions: Hens confined in the same cage group tend to develop similar microbiota in their ileum and caecum possibly due to isolation, while differences in the microbiota between cages may be caused by environmental or individual bird factors. Although the cages type had influence on composition of the microbiota in the layers by promoting higher diversity in furnished and aviary systems, we did not observe differences in colonization and excretion pattern of Salmonella from these groups. We suggest, that differences in group size and exposure to a more faecally contaminated environment in the alternative systems may explain the observed differences in diversity of the caecal microbiota. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5156665 Sun, 21 Aug 2011 23:00:00 +0100 5156665 Identification of one critical amino acid that determines a conformational neutralizing epitope in the capsid protein of porcine circovirus type 2 http://www.medworm.com/index.php?rid=5156664&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F188 Conclusions: It is concluded that the alanine at position 59 in the capsid protein of PCV2a (CL, LG and JF2) strains is a critical amino acid, which determines one neutralizing epitope of PCV2a (CL, LG and JF2) strains. This study provides valuable information for further in-depth mapping of the conformational neutralizing epitope, understanding antigenic difference among PCV2 strains, and development of a useful vaccine for control of PCV2-associated disease. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5156664 Sun, 21 Aug 2011 23:00:00 +0100 5156664 In Mycoplasma hominis the OppA-mediated cytoadhesion depends on its ATPase activity http://www.medworm.com/index.php?rid=5156667&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F185 Conclusions: These findings suggest that the OppA-mediated cytoadherence of Mycoplasma hominis depends on both, the topology of the neighbouring CS1 and ATPase domain regions and the functionality of the ecto-ATPase activity in addition. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5156667 Thu, 18 Aug 2011 23:00:00 +0100 5156667 Identification, structure, and characterization of an exopolysaccharide produced by Histophilus somni during biofilm formation http://www.medworm.com/index.php?rid=5156666&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F186 Conclusions: H. somni is capable of producing a branching, mannose-galactose EPS polymer under growth conditions favorable to the biofilm phase of growth, and the EPS is a component of the biofilm matrix. The EPS can be sialylated in strains with sialyltransferase activity, resulting in enhanced density of the biofilm, and suggesting that EPS and biofilm formation may be important to persistence in the bovine host. The EPS may be critical to virulence if the biofilm state is required for H. somni to persist in systemic sites. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5156666 Thu, 18 Aug 2011 23:00:00 +0100 5156666 Bacterial adaptation during chronic infection revealed by independent component analysis of transcriptomic data http://www.medworm.com/index.php?rid=5156668&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F184 Conclusions: Our results show that P. aeruginosa employs multiple patient-specific adaption strategies during the early stage infections while certain essential adaptations are evolved in parallel during the chronic infections. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5156668 Wed, 17 Aug 2011 23:00:00 +0100 5156668 Expression of urease by Haemophilus influenzae during human respiratory tract infection and role in survival in an acid environment http://www.medworm.com/index.php?rid=5138051&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F183 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5138051 Mon, 15 Aug 2011 23:00:00 +0100 5138051 Physiologic cold shock of Moraxella catarrhalis affects the expression of genes involved in the iron acquisition, serum resistance and immune evasion http://www.medworm.com/index.php?rid=5124712&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F182 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5124712 Thu, 11 Aug 2011 23:00:00 +0100 5124712 Effects of bacteriophage traits on plaque formation http://www.medworm.com/index.php?rid=5117884&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F181 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5117884 Mon, 08 Aug 2011 23:00:00 +0100 5117884 Isolates from hospital environments are the most virulent of the Candida parapsilosis complex http://www.medworm.com/index.php?rid=5117885&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F180 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5117885 Sun, 07 Aug 2011 23:00:00 +0100 5117885 A galU Mutant of Francisella tularensis is Attenuated for Virulence in a Murine Pulmonary Model of Tularemia http://www.medworm.com/index.php?rid=5103044&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F179 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5103044 Thu, 04 Aug 2011 23:00:00 +0100 5103044 Bifidobacterium pseudolongum are efficient indicators of animal fecal contamination in raw milk cheese industry http://www.medworm.com/index.php?rid=5094734&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F178 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5094734 Wed, 03 Aug 2011 23:00:00 +0100 5094734 A simplified and cost-effective enrichment protocol for the isolation of Campylobacter spp. from retail broiler meat without microaerobic incubation http://www.medworm.com/index.php?rid=5094737&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F175 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5094737 Tue, 02 Aug 2011 23:00:00 +0100 5094737 Identification of new IS711 insertion sites in Brucella abortus field isolates http://www.medworm.com/index.php?rid=5094736&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F176 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5094736 Tue, 02 Aug 2011 23:00:00 +0100 5094736 Oral administration of a probiotic Lactobacillus modulates cytokine production and TLR expression improving the immune response against Salmonella enterica serovar Typhimurium infection in mice http://www.medworm.com/index.php?rid=5094735&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F177 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5094735 Tue, 02 Aug 2011 23:00:00 +0100 5094735 Factors influencing lysis time stochasticity in bacteriophage lambda http://www.medworm.com/index.php?rid=5094738&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F174 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5094738 Mon, 01 Aug 2011 23:00:00 +0100 5094738 The Respiratory Molybdo-Selenoprotein Formate Dehydrogenases of Escherichia coli have Hydrogen: Benzyl Viologen Oxidoreductase Activity http://www.medworm.com/index.php?rid=5085472&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F173 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5085472 Sun, 31 Jul 2011 23:00:00 +0100 5085472 Comparative proteomic profiles of Aspergillus fumigatus and Aspergillus lentulus strains by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) http://www.medworm.com/index.php?rid=5076872&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F172 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5076872 Wed, 27 Jul 2011 23:00:00 +0100 5076872 A Multi Locus Variable Number of Tandem Repeat Analysis (MLVA) scheme for Streptococcus agalactiae genotyping http://www.medworm.com/index.php?rid=5068870&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F171 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5068870 Tue, 26 Jul 2011 23:00:00 +0100 5068870 The cross-pathway control system regulates production of the secondary metabolite toxin, sirodesmin PL, in the ascomycete, Leptosphaeria maculans http://www.medworm.com/index.php?rid=5068872&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F169 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5068872 Mon, 25 Jul 2011 23:00:00 +0100 5068872 The ColRS system is essential for the hunger response of glucose-growing Pseudomonas putida http://www.medworm.com/index.php?rid=5068871&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F170 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5068871 Mon, 25 Jul 2011 23:00:00 +0100 5068871 A protocol for enumeration of aquatic viruses by epifluorescence microscopy using AnodiscTM 13 membranes http://www.medworm.com/index.php?rid=5068873&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F168 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5068873 Sun, 24 Jul 2011 23:00:00 +0100 5068873 Campylobacter jejuni dsb gene expression is regulated by iron in a Fur-dependent manner and by a translational coupling mechanism http://www.medworm.com/index.php?rid=5061675&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F166 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5061675 Sun, 24 Jul 2011 23:00:00 +0100 5061675 Identification of a human immunodominant T-cell epitope of Mycobacterium tuberculosis antigen PPE44 http://www.medworm.com/index.php?rid=5061674&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F167 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5061674 Sun, 24 Jul 2011 23:00:00 +0100 5061674 SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes http://www.medworm.com/index.php?rid=5048888&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F164 ${item.shortDescription} (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5048888 Tue, 19 Jul 2011 23:00:00 +0100 5048888 Calcium/calmodulin kinase1 and its relation to thermotolerance and HSP90 in Sporothrix schenckii: an RNAi and yeast two-hybrid study http://www.medworm.com/index.php?rid=5018592&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F162 Conclusions: Using the RNAi technology we silenced the expression of sscmk1 gene in this fungus. RNAi transformants were unable to grow as yeast cells at 35 degrees C showing decreased tolerance to this temperature. The interaction of SSCMK1 with HSP90, observed using the yeast two-hybrid assay suggests that this kinase is involved in thermotolerance through its interaction with HSP90. SSCMK1 interacted with the C terminal domain of HSP90 where effector proteins and co-chaperones interact. These results confirmed SSCMK1 as an important enzyme involved in the dimorphism of S. schenckii, necessary for the development of the yeast phase of this fungus. Also this study constitutes the first report of the transformation of S. schenckii and the use of RNAi to study gene function in this fungus. ... BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5018592 Sun, 10 Jul 2011 23:00:00 +0100 5018592 Specific antibody-receptor interactions trigger InlAB-independent uptake of Listeria monocytogenes into tumor cell lines http://www.medworm.com/index.php?rid=5018591&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F163 Conclusions: Binding of receptor-specific antibodies to SPA-expressing L. monocytogenes may represent a promising approach to target L. monocytogenes to host cells expressing specific receptors triggering internalization. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5018591 Sun, 10 Jul 2011 23:00:00 +0100 5018591 Genetic diversity of Streptococcus suis isolates as determined by comparative genome hybridization http://www.medworm.com/index.php?rid=5007088&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F161 Conclusions: In conclusion, we show that CGH is a valuable method to study distribution of genes or gene clusters among isolates in detail, yielding information on genetic similarity, and virulence traits of S. suis isolates. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5007088 Wed, 06 Jul 2011 23:00:00 +0100 5007088 Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library http://www.medworm.com/index.php?rid=5007089&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F160 Conclusions: We identified TATTEK and VVDGPETKEC as NS1-specific linear B-cell epitopes recognized by the mAbs 3C7 and 4D1, respectively. The knowledge and reagents generated in this study may have potential applications in differential diagnosis and the development of epitope-based marker vaccines against WNV and other viruses of JEV serocomplex. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5007089 Tue, 05 Jul 2011 23:00:00 +0100 5007089 Phenotypic and Genotypic Characterization of Stenotrophomonas maltophilia Isolates from Patients with Cystic Fibrosis: Genome Diversity, Biofilm Formation, and Virulence. http://www.medworm.com/index.php?rid=5007090&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F159 Conclusions: Overall, the phenotypic differences observed between CF and non-CF isolates may imply different selective conditions and persistence (adaptation) mechanisms in a hostile and heterogeneous environment such as CF lung. Molecular elucidation of these mechanisms will be essential to better understand the selective adaptation in CF airways in order to design improved strategies useful to counteract and eradicate S. maltophilia infection. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5007090 Mon, 04 Jul 2011 23:00:00 +0100 5007090 The replication origin of a repABC plasmid http://www.medworm.com/index.php?rid=4992074&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F158 Conclusions: RepC is the only element encoded in the repABC operon of the R. etli p42d plasmid that is necessary and sufficient for plasmid replication and is probably the initiator protein. The oriV of this plasmid resides within the repC gene and is located close to or inside of a large A+T region. RepC can act as an incompatibility factor, and the last 39 amino acid residues of the carboxy-terminal region of this protein are involved in promoting this phenotype. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4992074 Wed, 29 Jun 2011 23:00:00 +0100 4992074 Evaluation of direct inoculation of the BD PHOENIX system from positive BACTEC blood cultures for both Gram-positive cocci and Gram-negative rods http://www.medworm.com/index.php?rid=4983901&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F156 Conclusions: Inoculation of Phoenix panels directly from positive blood cultures can be used to report reliable results of AST of GNR a day earlier, as well as ID-results of Enterobacteriaceae. For Staphylococcus and Enterococcus species, results of AST can also be reported a day earlier for all antibiotics, except for erythromycin and trimethoprim-sulfamethoxazole. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4983901 Wed, 29 Jun 2011 23:00:00 +0100 4983901 Antagonistic effect of Lactobacillus strains against gas-producing coliforms isolated from colicky infants http://www.medworm.com/index.php?rid=4983900&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F157 Conclusions: This study shows that two out of 27 strains of Lactobacillus examined possess an antimicrobial effect against six species of gas-forming coliforms isolated from colicky infants. Our findings may stimulate new researches to identify which Lactobacillus strains can improve colicky symptoms by acting on coliforms gut colonization. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4983900 Wed, 29 Jun 2011 23:00:00 +0100 4983900 In-vitro and in-vivo phenotype of type Asia 1 foot-and-mouth disease viruses utilizing two non-RGD receptor recognition sites http://www.medworm.com/index.php?rid=4983902&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F154 Conclusions: FMDV quasispecies evolving in a different biological environment gained the capability of selecting different receptor recognition site. The RDD-containing FMD viral genome can accommodate substitutions in the receptor binding site without additional changes in the capsid. The viruses expressing non-RGD receptor binding sites can replicate stably in vitro and produce typical FMD clinical disease in susceptible animals. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4983902 Tue, 28 Jun 2011 23:00:00 +0100 4983902 A multiplex real-time PCR assay targeting virulence and resistance genes in Salmonella enterica serotype Typhimurium. http://www.medworm.com/index.php?rid=4975035&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F151 Conclusion: The GeneDisc(R) assay developed in this study made it easier to explore variability within serotype Typhimurium by analyzing ten relevant gene determinants in a large collection of strains. This real-time multiplex method constitutes a valuable tool for strains characterization on epidemiological purposes. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4975035 Sun, 26 Jun 2011 23:00:00 +0100 4975035 Lactobacillus plantarum (VR1) isolated from an Ayurvedic medicine (Kutajarista) ameliorates in vitro cellular damage caused by Aeromonas veronii http://www.medworm.com/index.php?rid=4975034&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F152 Conclusions: This is the first study to report isolation of L. plantarum VR1 from Kutajarista and characterisation for its probiotic attributes. Our study demonstrates the antagonistic property of VR1 to A. veronii and effect of VR1 CFS in reduction of cellular damage caused by A. veronii in both Vero and MDCK cell lines. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4975034 Sun, 26 Jun 2011 23:00:00 +0100 4975034 Molecular and Comparative Analysis of Salmonella enterica Senftenberg from Humans and Animals using PFGE, MLST and NARMS. http://www.medworm.com/index.php?rid=4975033&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F153 Conclusion: The data demonstrates the circulation of at least two strain types in both animal and human health suggesting that S. Senftenberg is relatively homogeneous in its distribution. The data generated in this study could be used towards defining a pathotype for this serovar. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4975033 Sun, 26 Jun 2011 23:00:00 +0100 4975033 Characterization of surface proteins of Cronobacter muytjensii using monoclonal antibodies and MALDI-TOF Mass spectrometry http://www.medworm.com/index.php?rid=4967950&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F148 Conclusions: Results obtained in this study highlight the immunological cross-reactivity among Cronobacter OMPs and their Enterobacteriaceae counterparts. Nevertheless, the identity of the identified proteins appeared to be different as inferred from the MALDI-TOF sequencing and identification. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4967950 Fri, 24 Jun 2011 23:00:00 +0100 4967950 The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium plasmids and the chromosome of a Sinorhizobium strain. http://www.medworm.com/index.php?rid=4967949&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F149 Conclusions: S. fredii GR64 contains a chimeric transmissible plasmid, with segments from two R. etli plasmids and a S. fredii chromosome, and a symbiotic plasmid different from the one usually found in R. etli bv phaseoli. We infer that these plasmids originated through the transfer of a symbiotic-conjugative-plasmid cointegrate from R. etli to a S. fredii strain, and at least two recombination events among the R. etli plasmids and the S. fredii genome. As in R. etli CFN42, the S. fredii GR64 transmissible plasmid is required for the conjugative transfer of the symbiotic plasmid. In spite of the similarity in the conjugation related genes, the transfer process of these plasmids shows a host-specific behaviour. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4967949 Fri, 24 Jun 2011 23:00:00 +0100 4967949 Modulation of the Chlamydia trachomatis in vitro transcriptome response by the sex hormones estradiol and progesterone http://www.medworm.com/index.php?rid=4967948&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F150 Conclusion: Our data shows for the first time, that the treatment of chlamydial host cells with key reproductive hormones such as progesterone and estradiol, results in significantly altered chlamydial gene expression profiles. It is likely that these chlamydial expression patterns are survival responses, evolved by the pathogen to enable it to overcome the host's innate immune response. The induction of chlamydial persistence is probably a key component of this survival response. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4967948 Fri, 24 Jun 2011 23:00:00 +0100 4967948 Global transcriptome response in Lactobacillus sakei during growth on ribose http://www.medworm.com/index.php?rid=4967953&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F145 Conclusions: Our data shows that the ribose uptake and catabolic machinery in L. sakei is highly regulated at the transcription level. A global regulation mechanism seems to permit a fine tuning of the expression of enzymes that control efficient exploitation of available carbon sources. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4967953 Thu, 23 Jun 2011 23:00:00 +0100 4967953 Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation http://www.medworm.com/index.php?rid=4967952&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F146 Conclusions: Deletion of saeRS in S. epidermidis resulted in an increase in biofilm-forming ability, which was associated with increased eDNA release and up-regulated Aap expression. The increased eDNA release from SE1457DeltasaeRS was associated with increased bacterial autolysis and decreased bacterial cell viability in the planktonic/biofilm states. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4967952 Thu, 23 Jun 2011 23:00:00 +0100 4967952 In vivo versus in vitro protein abundance analysis of Shigella dysenteriae type 1 reveals changes in the expression of proteins involved in virulence, stress and energy metabolism http://www.medworm.com/index.php?rid=4967951&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F147 Conclusions: Global proteomic profiling of SD1 comparing in vivo vs. in vitro proteomes revealed differential expression of proteins geared towards survival of the pathogen in the host gut environment, including increased abundance of proteins involved in anaerobic energy respiration, acid resistance and virulence. The immunogenic OspC2, OspC3 and IpgA virulence proteins were detected solely under in vivo conditions, lending credence to their candidacy as potential vaccine targets. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4967951 Thu, 23 Jun 2011 23:00:00 +0100 4967951 Bacterial membrane activity of alpha-peptide/beta-peptoid chimeras: Influence of amino acid composition and chain length on the activity against different bacterial strains http://www.medworm.com/index.php?rid=4959528&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F144 Conclusion: We found that chain length but not type of cationic amino acid influenced the antibacterial activity of a series of synthetic alpha-peptide/beta-peptoid chimeras. The synthetic chimeras exert their killing effect by permeabilization of the bacterial cell envelope, and the outer membrane may act as a barrier in Gram-negative bacteria. The tolerance of S. marcescens to chimeras may be due to differences in the composition of the lipopolysaccharide layer also responsible for its resistance to polymyxin B. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4959528 Tue, 21 Jun 2011 23:00:00 +0100 4959528 Virulence gene profiling of enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains: a basis for molecular risk assessment of typical and atypical EPEC strains http://www.medworm.com/index.php?rid=4951731&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F142 Conclusion: The OI-122 encoded nleB gene was found to be most closely associated with Cluster 1 strains and may serve as a diagnostic tool for the identification of virulent EHEC and EPEC seropathotypes. OI-71 encoded genes nleA, nleF and nleH1-2 are less associated with Cluster 1 strains. EHEC-plasmid, OI-57 and CP-933 associated genes showed only weak similarities with virulent Cluster 1 EHEC and EPEC strains. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4951731 Mon, 20 Jun 2011 23:00:00 +0100 4951731 Staphylococcus aureus Biofilm and Planktonic Cultures Differentially Impact Gene Expression, MAPK Phosphorylation, and Cytokine Production in Human Keratinocytes http://www.medworm.com/index.php?rid=4951730&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F143 Conclusions: Collectively the results indicate that S. aureus biofilms induce a distinct inflammatory response compared to their planktonic counterparts. The differential gene expression and production of inflammatory cytokines by biofilm and planktonic cultures in keratinocytes could have implications for the formation and persistence of chronic wounds. The formation of a biofilm should be considered in any study investigating host response to bacteria. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4951730 Mon, 20 Jun 2011 23:00:00 +0100 4951730 The non-motile phenotype of Salmonella hha ydgT mutants is mediated through PefI-SrgD http://www.medworm.com/index.php?rid=4951732&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F141 Conclusion: These data extend our current understanding of the mechanisms through which Hha and YdgT regulate flagellar biosynthesis and further describe how S. Typhimurium has integrated horizontal gene acquisitions into ancestral regulatory networks. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4951732 Sun, 19 Jun 2011 23:00:00 +0100 4951732 Lytic activity of the virion-associated peptidoglycan hydrolase HydH5 of Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 http://www.medworm.com/index.php?rid=4944407&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F138 Conclusions: The virion-associated PG hydrolase HydH5 has lytic activity against S. aureus, which makes it attractive as antimicrobial for food biopreservation and anti-staphylococcal therapy. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4944407 Thu, 16 Jun 2011 23:00:00 +0100 4944407 Phylogeography of Francisella tularensis subspecies holarctica from the country of Georgia http://www.medworm.com/index.php?rid=4944406&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F139 Conclusion: We identified a new lineage of F. tularensis subsp. holarctica from Georgia that appears to have an older origin than any other diversified lineages previously described from the B.Br.013 group. This finding suggests that additional phylogenetic studies of F. tularensis subsp. holarctica populations in Eastern Europe and Asia have the potential to yield important new insights into the evolutionary history and phylogeography of this broadly dispersed F. tularensis subspecies. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4944406 Thu, 16 Jun 2011 23:00:00 +0100 4944406 High-resolution time series of Pseudomonas aeruginosa gene expression and rhamnolipid secretion through growth curve synchronization http://www.medworm.com/index.php?rid=4944405&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F140 Conclusions: Growth curve synchronization allows integration of rich time-resolved data with endpoint measurements to produce time-resolved quantitative measurements. Such data can be valuable to unveil the dynamic regulation of virulence in P. aeruginosa. More generally, growth curve synchronization can be applied to many biological systems thus helping to overcome a key obstacle in dynamic regulation: the scarceness of quantitative time-resolved data. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4944405 Thu, 16 Jun 2011 23:00:00 +0100 4944405 Enrichment of a microbial community performing anaerobic oxidation of methane in a continuous high-pressure bioreactor http://www.medworm.com/index.php?rid=4932924&cid=s_34035_77_f&fid=34035&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2180%2F11%2F137 Conclusion: This paper reports a net biomass growth of communities involved in SR-AOM, incubated at high-pressure. (Source: BMC Microbiology - Latest articles) BMC Microbiology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4932924 Wed, 15 Jun 2011 23:00:00 +0100 4932924