MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'BMC Molecular Biology - Latest articles' source. Thu, 09 Feb 2012 16:56:38 +0100 http://www.medworm.com/index.php?rid=5656507&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F13%2F4 Conclusions: Our work indicates a novel mechanism of miR-34a regulation that NF-kappaB could elevate miR-34a expression levels through directly binding to its promoter. And wildtype p53 is responsible for NF-kappaB-mediated miR-34a transcriptional activity but not for NF-kappaB binding. These findings might be helpful in understanding miR-34a abnormality in human malignancies and open new perspectives for the roles of miR-34a and NF-kappaB in tumor progression. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5656507 Tue, 31 Jan 2012 05:00:00 +0100 5656507 http://www.medworm.com/index.php?rid=5624810&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F13%2F3 Conclusions: Normal development of zebrafish embryos requires ZNF143. Furthermore, the pax2a gene is probably one example of many protein-coding gene targets of ZNF143 during zebrafish development. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5624810 Mon, 23 Jan 2012 05:00:00 +0100 5624810 http://www.medworm.com/index.php?rid=5604037&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F13%2F2 Conclusions: Our study is the first characterization of the possible role of AREs and other suppressive regions in the 3'-UTR in regulating the amounts of GDNF mRNA in C6 cells. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5604037 Tue, 17 Jan 2012 05:00:00 +0100 5604037 http://www.medworm.com/index.php?rid=5604038&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F13%2F1 Conclusions: The Drosophila bioassay system successfully demonstrated that some plant SPO11 orthologues have intrinsic DSB activities. Furthermore, we identified a novel SPO11 homologue, OsSPO11D, with robust DSB activity and a possible meiotic function. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5604038 Mon, 16 Jan 2012 05:00:00 +0100 5604038 http://www.medworm.com/index.php?rid=5533460&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F52 Conclusions: Our results provide the first characterization of Dlk2 transcripts, map the location of the Dlk2 core promoter, and show the role of Sp1 as a key regulator of Dlk2 transcription, providing new insights into the molecular mechanisms that contribute to the expression of the Dlk2 gene. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5533460 Tue, 20 Dec 2011 05:00:00 +0100 5533460 http://www.medworm.com/index.php?rid=5447663&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F51 Conclusions: The data suggest Ste11p regulates the FKS2 gene through all its known and putative downstream kinase substrates (Pbs2p, Ste7p, Cmk2p, and Ptk2p) and separately through Mpk1p MAPK. The patterns of control by Ste11p targets revealed novel functional linkages, cross-regulation, redundancy and compensation. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5447663 Thu, 24 Nov 2011 05:00:00 +0100 5447663 http://www.medworm.com/index.php?rid=5437290&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F50 Conclusions: The results strongly suggest that most Francisella RNAP contains alpha heterodimer with a minor subfraction possibly containing alpha1 homodimer. Comparative sequence analysis suggests that this heterodimer is oriented, in a sense that only one monomer, alpha1, interacts with the beta subunit during the (alpha)2/beta RNAP subassembly formation. Most likely the two rpoA copies in Francisella have emerged through a lineage-specific duplication followed by subfunctionalization of interacting paralogs. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5437290 Tue, 22 Nov 2011 05:00:00 +0100 5437290 http://www.medworm.com/index.php?rid=5428088&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F49 Conclusion: The high throughput capacity of Matrix ChIP-MeDIP to profile tens and potentially hundreds of different genomic events at the same time as DNA methylation represents a powerful platform to explore complex genomic mechanism at selected genes in cultured cells and in whole tissues. In this regard, Matrix ChIP-MeDIP should be useful to complement genome-wide studies where the rich chromatin and transcription database resources provide fruitful foundation to pursue mechanistic, functional and diagnostic information at genes of interest in health and disease. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5428088 Fri, 18 Nov 2011 05:00:00 +0100 5428088 http://www.medworm.com/index.php?rid=5417657&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F48 Conclusions: These results indicate that the recruitment of Stau2 alone or in combination with Upf1 differentially affects the fate of mRNAs. Moreover, the results suggest that Stau2-mediated fate determination could be executed in a cell type-specific manner. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5417657 Wed, 16 Nov 2011 05:00:00 +0100 5417657 http://www.medworm.com/index.php?rid=5396828&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F47 Conclusion: Our results highlight an important immune role of the defensin gene in Spodoptera littoralis by cooperating with other AMPs to control bacterial infection. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5396828 Wed, 09 Nov 2011 05:00:00 +0100 5396828 http://www.medworm.com/index.php?rid=5375444&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F46 Conclusions: We have shown that SWI/SNF can modulate alternative pre-mRNA processing, not only in cultured cells but also in vivo. The effect is restricted to and specific for a subset of transcripts. Our results provide novel insights into the mechanisms by which SWI/SNF regulates transcript diversity and proteomic diversity in higher eukaryotes. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5375444 Wed, 02 Nov 2011 04:00:00 +0100 5375444 http://www.medworm.com/index.php?rid=5330678&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F45 Conclusions: These results identify a domain encompassing the TPR of hEST1A as an hTERT interaction module. The TPR of S. cerevisiae Est1 is required for telomerase-mediated telomere length maintenance in a manner that appears separable from telomere recruitment. Discrete residues in or adjacent to the TPR of Est1 also regulate telomere length homeostasis. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5330678 Tue, 18 Oct 2011 04:00:00 +0100 5330678 http://www.medworm.com/index.php?rid=5321532&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F44 Conclusions: Determination of the optimal promoter region and transcription factors governing CD164 expression is useful in understanding CD164 functions. These results suggest that cis-regulatory elements of CD164 overlapping HIF-1alpha/E-box / AP-1-like sequences may play important regulatory roles. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5321532 Fri, 14 Oct 2011 04:00:00 +0100 5321532 http://www.medworm.com/index.php?rid=5268664&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F43 Conclusions: The binding to DNA, otherwise never reported for any other psi protein, enhances the affinity of P. aeruginosa chi psi towards the SSB/ssDNA complex and very likely contributes to the higher stimulatory effect of P. aeruginosa chi psi on the clamp loader. We also observed DNA-binding activity for P. putida chi psi, making this activity most probably a characteristic of the psi proteins from the Pseudomonadaceae. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5268664 Wed, 28 Sep 2011 04:00:00 +0100 5268664 http://www.medworm.com/index.php?rid=5233504&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F42 Conclusions: The activation of the bop promoter was shown to be dependent not only on two major factors, Bat and Brz, but is also tuned by the small basic protein, Brb. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5233504 Mon, 19 Sep 2011 04:00:00 +0100 5233504 http://www.medworm.com/index.php?rid=5233505&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F41 Conclusion: We have identified a critical role of LIMK1-mediated CFL1 pathway and actin dynamics in modulating retinoid receptor mediated function and shown that LIMK1-mediated phosphocycling of CFL1 plays a crucial role in maintaining actin homeostasis and receptor activity. We suggest that T cell activation-induced repression of nuclear receptor-dependent transactivation is in part through the modification of actin dynamics. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5233505 Fri, 16 Sep 2011 04:00:00 +0100 5233505 http://www.medworm.com/index.php?rid=5155677&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F40 Conclusions: This is the first report on the profile of sRNAs in a plant infected with a virus from the family Luteoviridae. Our sequence data strongly suggest that virus-derived double-stranded RNA functions as one of the main precursors of vsRNAs. Judging by the profiled size classes, all cotton DCLs might be working to silence the virus. The possible causes for the unexpectedly high accumulation of 22-nt vsRNAs are discussed. CLRDV is the causal agent of Cotton blue disease, which occurs worldwide. Our results are an important contribution for understanding the molecular mechanisms involved in this and related diseases. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5155677 Tue, 23 Aug 2011 23:00:00 +0100 5155677 http://www.medworm.com/index.php?rid=5155679&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F38 Conclusion. The PHMB treatment of the yeast cells activates the PKC1/Slt2 (CWI) pathway. In addition, it is suggested that HOG1 and YAP1 can play a role in the regulation of CWI genes (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5155679 Thu, 18 Aug 2011 23:00:00 +0100 5155679 http://www.medworm.com/index.php?rid=5155678&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F39 The tammar wallaby transient receptor potential channel, subfamily C, member 2 (TRPC2) locus, previously defined as a single gene in mouse, in fact consists of two distinct genes and this arrangement is conserved in all known vertebrates. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5155678 Thu, 18 Aug 2011 23:00:00 +0100 5155678 http://www.medworm.com/index.php?rid=5155680&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F37 Conclusions: Our results indicate that the inducible ES cell line system serves as a suitable in vitro system to identify the mRNA targets of DND1. The RIP-RT results hint at the broad spectrum of mRNA targets that interact with DND1 in ES cells. Based on what is known about DND1 function, our results suggest that DND1 may impose another level of translational regulation to modulate expression of critical factors in ES cells. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5155680 Wed, 17 Aug 2011 23:00:00 +0100 5155680 http://www.medworm.com/index.php?rid=5137855&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F36 ${item.shortDescription} (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5137855 Tue, 16 Aug 2011 23:00:00 +0100 5137855 http://www.medworm.com/index.php?rid=5117130&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F35 ${item.shortDescription} (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5117130 Sun, 07 Aug 2011 23:00:00 +0100 5117130 http://www.medworm.com/index.php?rid=5106118&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F34 ${item.shortDescription} (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5106118 Sun, 07 Aug 2011 23:00:00 +0100 5106118 http://www.medworm.com/index.php?rid=5102769&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F33 ${item.shortDescription} (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5102769 Thu, 04 Aug 2011 23:00:00 +0100 5102769 http://www.medworm.com/index.php?rid=5085273&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F32 ${item.shortDescription} (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5085273 Sun, 31 Jul 2011 23:00:00 +0100 5085273 http://www.medworm.com/index.php?rid=5048327&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F31 ${item.shortDescription} (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5048327 Mon, 18 Jul 2011 23:00:00 +0100 5048327 http://www.medworm.com/index.php?rid=5017847&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F30 Conclusions: The data suggest: (i) extensive NMD-sensitive transcripts of TRPC1; (ii) inefficient clearance of aberrant transcripts and enhanced proliferation of vascular smooth muscle cells in part because of low UPF1 expression. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5017847 Mon, 11 Jul 2011 23:00:00 +0100 5017847 http://www.medworm.com/index.php?rid=5017848&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F29 Conclusions: Our study establishes a mechanistic link between GRPR activation and enhanced COX-2 expression in prostate cancer cell lines, and suggests that inhibiting GRPR may, in the future, provide an effective therapeutic alternative to non-steroidal anti-inflammatory drugs for inhibiting COX-2 in patients with recurrent prostate cancer. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5017848 Sun, 10 Jul 2011 23:00:00 +0100 5017848 http://www.medworm.com/index.php?rid=5006358&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F28 Conclusions: Our results show that CGGBP1 expression is important for cell cycle progression through multiple parallel mechanisms including the regulation of CDKN1A and GAS1 levels. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=5006358 Wed, 06 Jul 2011 23:00:00 +0100 5006358 http://www.medworm.com/index.php?rid=4920019&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F27 Conclusions: Our observations suggest that i) some cationic liposomes may not be suitable for functional studies on hsp promoters, ii) lipofection may cause unintended changes in global gene expression in the transfected cells. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4920019 Thu, 09 Jun 2011 23:00:00 +0100 4920019 http://www.medworm.com/index.php?rid=4861247&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F25 Conclusions: It was first found that the UPR mechanisms and the function of pXbp1 in the porcine system. These results indicate that pXbp1 plays an important role during the ER stress response like other animal systems and open a new opportunity for examining the UPR pathway in the porcine model system. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4861247 Mon, 23 May 2011 23:00:00 +0100 4861247 http://www.medworm.com/index.php?rid=4861246&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F26 Conclusions: Previous evidence on VAMP7 suggests inhibitory functions for the Longin domain and fusion/growth promoting activity for the Delta-longin molecule. Thus, non-SNARE isoforms with Longin domain and non-longin SNARE isoforms might have somehow opposite regulatory functions. When considering splice variants as "natural mutants", evidence on modulation of subcellular localization by variation in domain combination can shed further light on targeting determinants. Although further work will be needed to characterize identified variants, our data might open the route to unravel novel molecular partners and mechanisms, accounting for the multiplicity of functions carried out by the different members of the Longin proteins family. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4861246 Mon, 23 May 2011 23:00:00 +0100 4861246 http://www.medworm.com/index.php?rid=4861248&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F24 Conclusions: The excision in vivo of the RmInt1 intron is a favored process, with very few constraints for sequence recognition in both the 5' and 3'-exons. Our results contribute to understand how group II introns spread in nature, and might facilitate the use of RmInt1 in gene targeting. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4861248 Sun, 22 May 2011 23:00:00 +0100 4861248 http://www.medworm.com/index.php?rid=4839732&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F23 Conclusions: :Gene cluster instability, sensitivity to hmdUrd and sister chromatid exchange are mechanistically distinct phenomena. Genomic instability in the NCI-60 appears to involve only one mechanism of instability for each individual cell line. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4839732 Mon, 16 May 2011 23:00:00 +0100 4839732 http://www.medworm.com/index.php?rid=4827622&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F22 Conclusion: These results suggest that GhMPK16 might be involved in multiple signal transduction pathways, including biotic and abiotic stress signaling pathways. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4827622 Sun, 15 May 2011 23:00:00 +0100 4827622 http://www.medworm.com/index.php?rid=4827623&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F21 Conclusions: The results suggest that the open complex formation of the tetracycline inducible promoter, in the regime of strong induction, affects significantly the dynamics of RNA production due to the variability of its duration from one event to the next. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4827623 Fri, 13 May 2011 23:00:00 +0100 4827623 http://www.medworm.com/index.php?rid=4794214&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F20 Conclusions: These results suggest that MBNL1 is highly flexible and able to adopt different conformations to recognize RNAs with varying sequence configurations. Although MBNL1 contains four ZnFs, only two ZnF - GC dinucleotide interactions are necessary for high affinity binding. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4794214 Thu, 05 May 2011 23:00:00 +0100 4794214 http://www.medworm.com/index.php?rid=4744639&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F18 Conclusion: This study identified cysG/hcaT/idnT to be reliable novel reference genes for transcription analysis in recombinant protein producing E. coli. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4744639 Fri, 22 Apr 2011 23:00:00 +0100 4744639 http://www.medworm.com/index.php?rid=4741772&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F17 Conclusions: The characterization of DgeRecA and DmuRecA proteins made in this study indicates that the unique properties of D. radiodurans RecA are probably not common among RecA proteins from Deinococcus sp. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4741772 Thu, 21 Apr 2011 23:00:00 +0100 4741772 http://www.medworm.com/index.php?rid=4722969&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F16 Conclusions: The activities of the SXT-Exo protein are consistent with it having the ability to resect the ends of linearized dsDNA molecules, forming partially ssDNA substrates for the partnering SXT-Bet single strand annealing protein. As such, SXT-Exo and SXT-Bet may function together to repair or process SXT genetic elements within infected V. cholerae cells, through facilitating homologous DNA recombination events. The results presented here significantly extend our general understanding of the properties and activities of alkaline exonuclease and single strand annealing proteins of viral/bacteriophage origin, and will assist the rational development of bacterial recombineering systems. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4722969 Sun, 17 Apr 2011 23:00:00 +0100 4722969 http://www.medworm.com/index.php?rid=4703588&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F15 Conclusions: This study is the first report to employ cDNA microarray to study testicular development in the black tiger shrimp. We show that there are obvious differences between the wild and domesticated shrimp at the transcriptomic level. Furthermore, our study is the first to investigate the feasibility that the CSN gene family might have involved in reproduction and development of this economically important species. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4703588 Mon, 11 Apr 2011 23:00:00 +0100 4703588 http://www.medworm.com/index.php?rid=4703589&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F14 Conclusions: The conventional northern blotting enhanced to high resolution may be a useful adjunct to other miRNA discovery, detection and characterization methods. It provides quantitative data on distribution of major length variants of abundant endogenous miRNAs, as well as on length heterogeneity of RNAi technology reagents expressed in cells. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4703589 Sun, 10 Apr 2011 23:00:00 +0100 4703589 http://www.medworm.com/index.php?rid=4682876&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F13 Conclusions: Ctf4 remains a central player in DNA replication. Our findings are consistent with what has been previously reported for CTF4 function in yeast, Xenopus extracts, and human tissue culture. We show that Ctf4 function is conserved and that Drosophila can be effectively used as a model to further probe the precise function of Ctf4 as a member of the replication fork and possible roles in development. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4682876 Tue, 05 Apr 2011 23:00:00 +0100 4682876 http://www.medworm.com/index.php?rid=4663250&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F12 Conclusion: These observations provide new evidence for a role of GreA in resolving conflicts between replication and transcription. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4663250 Wed, 30 Mar 2011 23:00:00 +0100 4663250 http://www.medworm.com/index.php?rid=4535475&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F11 Conclusions: For the application of molecular bio-indication in environmental assessments, multivariate analyses obviously have an added value over univariate methods. Our results suggest that compound discrimination can be achieved by PLS-DA, based on a hard classification of the within-class rankings of samples from a test set. This study clearly shows that the use of high throughput RT-qPCR could be a valuable tool in ecotoxicology combining high throughput with analytical sensitivity. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4535475 Tue, 01 Mar 2011 00:00:00 +0100 4535475 http://www.medworm.com/index.php?rid=4527443&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F10 Conclusions: Smtnl1 transcript was identified in all smooth muscle tissues examined to date, albeit at much lower levels than found in skeletal muscle. It is unlikely that multiple SMTNL1 isoforms exist since a single Smtnl1 transcription start site was identified in both skeletal and intestinal smooth muscle. Promoter studies suggest restrictive control of Smtnl1 expression in non-muscle cells. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4527443 Sun, 27 Feb 2011 00:00:00 +0100 4527443 http://www.medworm.com/index.php?rid=4488796&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F8 Conclusion: The data herein provide evidence that ACTB and TUBB1 are suitable reference genes in human skin fibroblasts irradiated by UVB, whereas VIM and TUBA1A are not and should therefore be excluded as reference genes in any gene expression studies involving UVB-irradiated human skin fibroblasts. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4488796 Thu, 17 Feb 2011 00:00:00 +0100 4488796 http://www.medworm.com/index.php?rid=4488797&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F7 Conclusion: Arm and EF1a is the most stably expressed combination of two reference genes of the eight examined for reliable normalization of RT-qPCR assays studying density-dependent behavioural change in the Australian plague locust. Such normalization allowed us to show that C. terminifera crowding did not change the neuronal expression of the SPARC gene, a gregarious phase-specific gene identified in brains of the desert locust, S. gregaria. Such comparative results on density-dependent gene regulation provide insights into the evolution of gregarious behaviour and mass migration of locusts. The eight identified genes we evaluated are also candidates as normalization genes for use in experiments involving other Oedipodinae species, but the rank order of gene stability must necessarily b... BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4488797 Wed, 16 Feb 2011 00:00:00 +0100 4488797 http://www.medworm.com/index.php?rid=4411092&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F5 Conclusion: The geometric mean of GAPDH, HPRT, SDHA and RPL32 is recommended for accurate normalization of quantitative PCR data in BAL cells of horses with IAD treated with corticosteroids. If only one reference gene can be used, then GAPDH is recommended. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4411092 Fri, 28 Jan 2011 00:00:00 +0100 4411092 http://www.medworm.com/index.php?rid=4370427&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F4 Conclusions: Our study provides evidence for physical and functional interactions between Rasd1 and Ear2. The results suggest that their interactions are involved in renin transcriptional regulation. These findings not only reveal a novel role for Rasd1-medated signaling but also provide the basis for potential intervention of renin expression. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4370427 Wed, 19 Jan 2011 00:00:00 +0100 4370427 http://www.medworm.com/index.php?rid=4342292&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F2 Conclusion: The high variability between strains supports the concept of E. siliculosus as a complex of cryptic species. Moreover, our data suggest that several parts of the Ectocarpus genome may have evolved at different rates: high variability was detected particularly in transposable elements and fucoxanthin chlorophyll a/c binding proteins. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4342292 Thu, 13 Jan 2011 00:00:00 +0100 4342292 http://www.medworm.com/index.php?rid=4317872&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F12%2F1 Conclusions: Our present data suggests that TEAD1 plays an important role in the regulation of gene expression and different signaling pathways may co-operate with each other mediated by TEAD1. We have preliminarily concluded that TEAD1 may regulate FoxO3a expression through calcineurin/MEF2/NFAT and IGF-1/PI3K/AKT signaling pathways in skeletal muscles. These findings provide important clues for further analysis of the role of FoxO3a gene in the formation and transformation of skeletal muscle fiber types. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4317872 Fri, 07 Jan 2011 00:00:00 +0100 4317872 http://www.medworm.com/index.php?rid=4301969&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F103 Conclusions: Our data demonstrates that transfected Rluc is the most appropriate normalization reference gene for transient transfection qPCR analysis; it significantly reduces the standard deviation within biological experiments as it takes into account the transfection efficiencies and has easily controllable expression levels. This improves reproducibility, data validity and most importantly, enables accurate interpretation of qPCR data. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4301969 Fri, 31 Dec 2010 00:00:00 +0100 4301969 http://www.medworm.com/index.php?rid=4301968&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F104 Conclusion: In summary, a daily oscillation of circadian clock genes and inflammatory cytokines as well as the ability of melatonin to function as a daily mediator of inflammation provides valuable information to aid in deciphering how the circadian timing system regulates immune function at the molecular level. However, further research is needed to clarify the precise mechanisms by which the circadian clock and melatonin have an impact upon daily immune functions in the periphery. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4301968 Fri, 31 Dec 2010 00:00:00 +0100 4301968 http://www.medworm.com/index.php?rid=4298313&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F102 Conclusions: Our results suggest that a single cleft in the homodimeric E. coli beta sliding clamp protein is sufficient to support both cell viability, as well as Pol III, Pol IV, and Pol V function in vivo. These findings provide further support for a model in which different Pols switch places with each other on DNA using a single cleft in the clamp. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4298313 Wed, 29 Dec 2010 00:00:00 +0100 4298313 http://www.medworm.com/index.php?rid=4277361&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F101 Conclusions: CTCF is able to form an unusual DNA structure through the zinc finger domain of the protein. This unusual DNA structure is formed in a directional manner by the CTCF protein. The findings described in this paper suggest mechanisms by which CTCF is able to form DNA loops, organize the mammalian genome and to function as an insulator protein. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4277361 Tue, 21 Dec 2010 00:00:00 +0100 4277361 http://www.medworm.com/index.php?rid=4274900&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F100 Conclusions: As the variability of expression levels for the reference genes is very high comparing fresh frozen and formalin-fixed tissue there is a strong need for validation in each tissue type. None of the alternative receptor tyrosine kinases analyzed is associated with the pathogenesis of wild-type or mutated GIST. It remains to be clarified whether an autocrine or paracrine mechanism by overexpression of receptor tyrosine kinase ligands is responsible for the tumorigenesis of wt-GIST. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4274900 Mon, 20 Dec 2010 00:00:00 +0100 4274900 http://www.medworm.com/index.php?rid=4264323&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F99 Conclusions: This study identified Thy-ncR1 ncRNA to be specifically expressed in stage III immature T cells in which the neighbouring CD1 gene cluster was activated. Complex alternative splicing produces multiple Thy-ncR1 isoforms. Two major Thy-ncR1 isoforms are cytoplasmic riboregulators that suppress the expression of MFAP4 mRNA, which is degraded by an uncharacterized hUPF1-dependent pathway. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4264323 Thu, 16 Dec 2010 00:00:00 +0100 4264323 http://www.medworm.com/index.php?rid=4250906&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F95 Conclusions: Our results show that the regulation of NTRK3 by microRNAs is isoform-specific and suggest that neurotrophin-mediated processes are strongly linked to microRNA-dependent mechanisms. In addition, these findings open new perspectives for the study of the physiological role of miR-128 and its possible involvement in cell death/survival processes. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4250906 Fri, 10 Dec 2010 00:00:00 +0100 4250906 http://www.medworm.com/index.php?rid=4250905&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F97 Conclusions: Our results strongly suggest that the variant nuclear receptor isoform, TR-alpha-2, is not expressed in marsupials and that the antisense overlap between TR-alpha-2 and Rev-erb-alpha thus is unique to eutherian mammals. Further investigation of the TR-alpha and Rev-erb-alpha genes in marsupial and eutherian species promises to yield additional insight into the physiological function of TR-alpha-2 and the role of the associated antisense overlap in regulating expression of these genes. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4250905 Fri, 10 Dec 2010 00:00:00 +0100 4250905 http://www.medworm.com/index.php?rid=4236860&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F94 Conclusions: The model provides a rationale for the observed products of the RAM reaction, and the molecular yield among those products. Experimental results are consistent with the model. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4236860 Tue, 07 Dec 2010 00:00:00 +0100 4236860 http://www.medworm.com/index.php?rid=4232702&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F93 Conclusions The results from the presented experiments demonstrated that selective gene knock-down in subsets of brain cells is achievable. However, there are some presently unknown regional factors which affect either the processing of miRNA-based cassettes or their potency for gene silencing. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4232702 Mon, 06 Dec 2010 00:00:00 +0100 4232702 http://www.medworm.com/index.php?rid=4228918&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F92 Conclusion: We propose as a mechanism, that upon rapamycin exposure Rrd1 isomerizes Rpb1 to promote its dissociation from the chromatin in order to modulate transcription. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4228918 Fri, 03 Dec 2010 00:00:00 +0100 4228918 http://www.medworm.com/index.php?rid=4218947&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F90 Conclusion: Consequently, it is recommended to normalize any real-time PCR-based expression data obtained during THP-1 monocyte differentiation using ACTB and RPL37A. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4218947 Wed, 01 Dec 2010 00:00:00 +0100 4218947 http://www.medworm.com/index.php?rid=4189333&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F89 Conclusions: This study demonstrates the capacity of PML to form macromolecular protein assemblies at several different subcellular sites. Further, it emphasizes a role of the variable C-terminus in subcellular target selection and a general role of the N-terminal TRIM domain in promoting protein clustering. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4189333 Sun, 21 Nov 2010 00:00:00 +0100 4189333 http://www.medworm.com/index.php?rid=4182559&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F87 Conclusions: The initiation of translation of pvuIIR appears to be independent of that for pvuIIC. Direct tests failed to detect regulatory rules for either gene overlap or the putative hairpins. Thus, at least during balanced growth, transcriptional control appears to be sufficiently robust for proper regulation of this RM system. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4182559 Fri, 19 Nov 2010 00:00:00 +0100 4182559 http://www.medworm.com/index.php?rid=4171469&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F86 Conclusions: We have performed stable germline transformation of a stalk-eyed fly, T. dalmanni. This is the first transgenic protocol to be developed in an insect species that exhibits an exaggerated male sexual trait. Transgenesis will enable the development of a range of techniques for analysing gene function in this species and so provide insight into the mechanisms underlying the development of a morphological trait subject to sexual selection. Our X-linked insertion line will permit the sex of live larvae to be determined. This will greatly facilitate the identification of genes which are differentially expressed during eye-stalk development in males and females. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4171469 Tue, 16 Nov 2010 00:00:00 +0100 4171469 http://www.medworm.com/index.php?rid=4167120&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F84 In this study we designed Group I introns to target and catalyze trans-splicing within the conserved sequences of the 5' CS region of DENV. These introns cleave either single stranded or homologously paired double stranded RNA at defined uracils and covalently join a 3' exon tag to the end of the cleavage product. We evaluated these introns for activity in both transfected and transformed cell cultures to determine their effectiveness in targeting DENV sequences. Two of these introns, designed 9v1 and 96v4 gave the greatest number of trans-splice product compared to the other Anti-DENV Group I trans-splicing introns (alphaDENV-GrpI) in each respective series, as judged by luciferase assays. The success of this approach against both subgenomic DENV sequences and infecting DENV genomes provi... BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4167120 Mon, 15 Nov 2010 00:00:00 +0100 4167120 http://www.medworm.com/index.php?rid=4162577&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F82 Conclusion: Depending on the cell type and preparation, validation of the harvesting technique is extremely important as contaminations may give false positives. Here we present an optimized protocol allowing secure harvesting of RNA from single cells in primary pituitary cell culture following perforated whole cell patch clamp experiments. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4162577 Fri, 12 Nov 2010 00:00:00 +0100 4162577 http://www.medworm.com/index.php?rid=4149828&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F80 Conclusions: Targeting MOF to reporter genes led to transcription enhancement and acetylation of histone H4 at lysine 16. Histone acetyltransferase activity was required for the full transcriptional response. Incorporation of Gal4-MOF into the MSL complex in males led to a lower transcription enhancement of UAS-DsRed but not UAS-arm-lacZ genes. We discuss how association of Gal4-MOF with the MSL or NSL proteins could explain our results. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4149828 Tue, 09 Nov 2010 00:00:00 +0100 4149828 http://www.medworm.com/index.php?rid=4149827&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F81 Conclusions: Results provide evidence for a novel mechanism of transcriptional control by multiple promoters used differently in various tissues and cells. Viral infection alters not only the use of PURA promoters but also the generation of different non-coding RNAs from 5'-UTRs of the resulting transcripts. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4149827 Tue, 09 Nov 2010 00:00:00 +0100 4149827 http://www.medworm.com/index.php?rid=4132527&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F79 Conclusions: Our data suggest that transcription activators USF1 and IRF1 interact to modulate CEACAM1 expression and that the chromatin structure of the promoter is likely maintained in a poised state that can promote rapid induction under appropriate conditions. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4132527 Thu, 04 Nov 2010 00:00:00 +0100 4132527 http://www.medworm.com/index.php?rid=4132528&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F78 Conclusions: We thus identified a new variant form of LYP and conducted a comprehensive analysis of LYP tissue expressions. Considering the pathogenesis of LYP R620W, we believe that the expression of LYP3 may have an important role in regulating activity and function of LYP and may be implicated in autoimmune diseases. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4132528 Tue, 02 Nov 2010 00:00:00 +0100 4132528 http://www.medworm.com/index.php?rid=4055337&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F77 Conclusions: By directly comparing the ability of shRNAs delivered from different co-RNA platforms to initiate knockdown of the same gene targets, we found that multiple U6/shRNA cassettes offered the most reliable and predictable suppression of both single and multiple-gene targets. These results highlight some important strengths and pitfalls of the currently used methods for multiple shRNA delivery, and provide valuable insights for the design and application of reliable co-RNAi. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4055337 Sun, 10 Oct 2010 23:00:00 +0100 4055337 http://www.medworm.com/index.php?rid=4035773&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F76 Conclusions: In this study, we validated the suitable reference genes for gene expression profiling in different tissues of B. dorsalis. Moreover, appropriate reference genes were selected out for gene expression profiling of the same tissues taking the sexual differences into consideration. This work not only formed a solid basis for future gene expression study in B. dorsalis, but also will serve as a resource to screen reference genes for gene expression studies in any other insects. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=4035773 Tue, 05 Oct 2010 23:00:00 +0100 4035773 http://www.medworm.com/index.php?rid=3992647&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F74 The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols is leading to the publication of papers that may not be technically appropriate. We take the view that this problem requires the delineation of a more transparent and comprehensive reporting policy from scientific journals. This editorial aims to provide practical guidance for the incorporation of absolute minimum standards encompassing the key assay parameters for accurate design, documentation and reporting of qPCR experiments (MIQE precis) and guidance on the publication of pure 'reference gene' articles. (Source: BMC Molecular Biology -... BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3992647 Mon, 20 Sep 2010 23:00:00 +0100 3992647 http://www.medworm.com/index.php?rid=3986429&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F73 Conclusion: Our study showed that expression stability varied between putative reference genes tested in E. globulus. Based on the AGO1 relative expression profile obtained using the genes suggested by the algorithms, H2B and TUA were considered as the most suitable reference genes for expression studies in E. globulus adventitious rooting. UBI and 18S were unsuitable for use as controls in qPCR related to this process. These findings will enable more accurate and reliable normalization of qPCR results for gene expression studies in this economically important woody plant, particularly related to rooting and clonal propagation. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3986429 Sun, 19 Sep 2010 23:00:00 +0100 3986429 http://www.medworm.com/index.php?rid=3976057&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F71 Conclusions: Evidence provided in the present report supports a role of Es-FABP in lipid transport during the period of rapid ovarian growth in E. sinensis, and indirectly confirms the participation of the hepatopancreas, ovary, and hemolymph in lipid nutrient absorption and utilization processes. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3976057 Wed, 15 Sep 2010 23:00:00 +0100 3976057 http://www.medworm.com/index.php?rid=3976056&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F72 Conclusions: : mTERF protein levels materially affect the amount of readthrough transcription on the antisense strand of mtDNA, whilst the effects on sense-strand transcripts are complex, and suggest the influence of compensatory mechanisms. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3976056 Wed, 15 Sep 2010 23:00:00 +0100 3976056 http://www.medworm.com/index.php?rid=3964958&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F69 Conclusions: Our results identify mCRY2 amino acid residues that interact with the mPER2 binding region and suggest the potential for rational drug design to inhibit CRYs for specific therapeutic approaches. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3964958 Mon, 13 Sep 2010 23:00:00 +0100 3964958 http://www.medworm.com/index.php?rid=3957363&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F68 Conclusions: Identification of Elf5-targets is an essential first step in elucidating the transcriptional landscape that is shaped by this important regulator. Our studies offer new toolbox in examining the biological role of Elf5 in mammary gland development and differentiation. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3957363 Thu, 09 Sep 2010 23:00:00 +0100 3957363 http://www.medworm.com/index.php?rid=3928529&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F67 Conclusion: Our study of the avian MYB/gga-miR-150 interaction shows a conservation of miR-150 target site functionality between chicken and zebrafish that does not extend to mammals. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3928529 Wed, 01 Sep 2010 23:00:00 +0100 3928529 http://www.medworm.com/index.php?rid=3920107&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F65 Conclusions: These findings provide new data on the tissue distribution, expression patterns and the function in starvation of storage proteins. RNA interference results revealed that storage protein genes are key in metamorphosis, reproduction and insect development. The results for SeHex and SeSP1 interference reveal that a potential method to control this pest is to disrupt the regulation of storage proteins. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3920107 Mon, 30 Aug 2010 23:00:00 +0100 3920107 http://www.medworm.com/index.php?rid=3920106&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F66 Conclusions: This study defines a novel mechanism of survivin regulation by EGF through the Raf-1/MEK/ERK pathway in pancreatic beta-cells, via prolongation of survivin protein half-life and inhibition of the ubiquitin-mediated proteasomal degradation pathway. This mechanism may be important for regulating beta-cell expansion after birth. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3920106 Mon, 30 Aug 2010 23:00:00 +0100 3920106 http://www.medworm.com/index.php?rid=3911206&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F64 Conclusions: Our results, for the first time, provide evidence that the L1CAM gene has two functionally active promoter sites that are used in a cell-type specific manner. Slug and beta-catenin are involved L1CAM transcriptional regulation. Nevertheless, Slug rather than beta-catenin levels are correlated with L1CAM expression in EC cell lines. Our findings suggest that the L1CAM transcriptional regulation is more complex than anticipated and this study provides the basis for a better understanding of L1CAM regulation in non-neuronal/tumor cells. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3911206 Thu, 26 Aug 2010 23:00:00 +0100 3911206 http://www.medworm.com/index.php?rid=3902464&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F63 Conclusion: Our results indicate that transcription factor activity and subcellular localization signals reside in two separate domains of the protein and both are essential for the correct functionality of RAI1. The pathogenic outcome of some of the mutated forms can be explained by the dissociation of these two domains. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3902464 Tue, 24 Aug 2010 23:00:00 +0100 3902464 http://www.medworm.com/index.php?rid=3876102&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F61 Conclusions: In order to make comparisons between heterogeneous MSC populations, as well as adult stem cell like MSC which are used in different laboratories throughout the world, it is important to have a standardized, reproducible set of housekeeping genes for RT-qPCR analysis. In this study we demonstrate that EF1alpha, RPL13a and YWHAZ are suitable genes for the RT-qPCR analysis and comparison of several sources of human MSC during in vitro characterization as well as in an in vivo animal model. This will allow for the comparative RT-qPCR analysis of multiple MSC populations with the goal of future use in animal models of disease as well as tissue repair. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3876102 Mon, 16 Aug 2010 23:00:00 +0100 3876102 http://www.medworm.com/index.php?rid=3871930&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F60 Conclusions: Genetic background and circadian time influence the expression stability of reference genes. Differences between mouse strains and tissues should be taken into consideration to avoid false interpretations. We show that the use of a single reference gene can lead to false biological conclusions. This manuscript provides a useful reference point for researchers that search for stable reference genes in the field of circadian biology. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3871930 Sun, 15 Aug 2010 23:00:00 +0100 3871930 http://www.medworm.com/index.php?rid=3864851&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F58 Background: It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the fad2 gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integration. Results: We confirmed that one fad2 line had a sole integration site on the X chromosome according to the inheritance patterns. Mapping of insertion sequences with thermal asymmetric interlaced and conventional PCR revealed that the foreign DNA was inserted into the XC1 region of the X chromosome by a homologous illegitimate replacement of an entire 45,556-bp endogenous genomic region, including the ovarian granulosa cell tumourigenesis-4 allele. For 5' and 3' junction se... BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3864851 Thu, 12 Aug 2010 23:00:00 +0100 3864851 http://www.medworm.com/index.php?rid=3857685&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F57 Conclusions: As we could show that gene expression results can vary depending on the approach used for quantification, we recommend to carefully evaluate different quantification approaches before using them in studies analysing gene expression. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3857685 Tue, 10 Aug 2010 23:00:00 +0100 3857685 http://www.medworm.com/index.php?rid=3853899&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F56 Conclusions: Several selected peptides showed ligand-specific changes in amino acid frequencies, but no consensus motif could be identified. This is consistent with previous observations on natural cis-acting peptides, showing that it is often impossible to demonstrate a consensus. Applying the currently developed method on a larger scale, by selecting and comparing an extended set of sequences, might allow the sequence rules underlying the activity of cis-acting regulatory peptides to be identified. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3853899 Mon, 09 Aug 2010 23:00:00 +0100 3853899 http://www.medworm.com/index.php?rid=3801859&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F54 Conclusions: Hence we propose a new model for dsDNA recombination, termed 'beta' recombination, based on the formation of ssDNA heteroduplexes at the replication fork. Implications of the model were tested using (i) an in situ assay for recombination, which showed that recombination generated mixed wild type and recombinant colonies; and (ii) the predicted asymmetries of the homology arms, which showed that recombination is more sensitive to non-homologies attached to 5' than 3' ends. Whereas beta recombination can generate deletions in target BACs of at least 50 kb at about the same efficiency as small deletions, the converse event of insertion is very sensitive to increasing size. Insertions up to 3 kb are most efficiently achieved using beta recombination, however at greater sizes, an a... BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3801859 Wed, 28 Jul 2010 23:00:00 +0100 3801859 http://www.medworm.com/index.php?rid=3743401&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F53 Conclusions: Our results demonstrate binding between endogenous GAS41 and the endogenous TFIIF subunits (RAP30 and RAP74). Since we did not find evidence for a binding of GAS41 to TBP or RNA polymerase II, GAS41 seems to preferentially bind to TFIIF. GAS41 that does not contain a DNA-binding domain appears to be a co-factor of TFIIF. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3743401 Sun, 11 Jul 2010 23:00:00 +0100 3743401 http://www.medworm.com/index.php?rid=3710001&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F50 Conclusions: Our results suggest that the genes Xbp1, Tbp, CAPON and Stx16 are suitable reference genes for accurate normalization in qRT-PCR studies using Chaoborus-induced D. pulex specimens. Furthermore, our study underscores the importance of verifying the suitability of reference genes identified by microarray analyses for normalization of expression levels. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3710001 Mon, 28 Jun 2010 23:00:00 +0100 3710001 http://www.medworm.com/index.php?rid=3710000&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F51 Conclusions: Our results describe two new transcriptional start sites in ACVRL1 gene, and indicate that Sp1 is a key regulator of ACVRL1 transcription, providing new insights into the molecular mechanisms that contribute to the expression of ACVRL1 gene. Moreover, our data show that the methylation status of CpG islands markedly modulates the Sp1 regulation of ACVRL1 gene transcriptional activity. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3710000 Mon, 28 Jun 2010 23:00:00 +0100 3710000 http://www.medworm.com/index.php?rid=3709999&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F52 Conclusions: Because of their utilisation in the in vivo modelling of disease, there is a requirement to develop molecular methods that accurately differentiate the different MHC mRNAs in the guinea pig to enable rapid profiling of muscle composition in appropriate disease models. The methods developed here are suitable for the characterisation of muscle MHC expression at the molecular level from animal tissue samples and biopsy material. The publication of these specific oligonucleotide primers for the guinea pig MHC variants will enable researchers to rapidly and accurately quantify acute changes in MHC mRNA expression in either developmental or in guinea pig disease models where a marker of altered skeletal muscle function is required. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3709999 Mon, 28 Jun 2010 23:00:00 +0100 3709999 http://www.medworm.com/index.php?rid=3694808&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F49 Conclusion: The study presents evidence that cell sensitization towards cisplatin by sodium butyrate is due to hyperacetylation of histone H4 in specific chromatin regions, which temporarily abrogates the cisplatin imposed cell cycle arrest. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3694808 Wed, 23 Jun 2010 23:00:00 +0100 3694808 http://www.medworm.com/index.php?rid=3690398&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F48 Conclusions: The results presented here indicate that a single hexameric consensus is sufficient for high-affinity RNA binding by STAR proteins, and that upstream or downstream partial consensus elements may alter binding affinities depending on the sequence and spacing. The general requirements determined for high-affinity RNA binding by STAR proteins will help facilitate the identification of novel regulatory targets in vivo. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3690398 Tue, 22 Jun 2010 23:00:00 +0100 3690398 http://www.medworm.com/index.php?rid=3683120&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F47 Conclusions: Using gene microarray analysis, we identified genes showing high expression stability under various sex-steroid environments in different regions of the rhesus macaque brain. Our approach of using quantile normalized microarray gene expression values represents an improvement over traditional methods of selecting internal reference genes for PCR analysis. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3683120 Sun, 20 Jun 2010 23:00:00 +0100 3683120 http://www.medworm.com/index.php?rid=3670886&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F45 Conclusions: We propose a more uniform nomenclature for alternative exons in Enah. We provide an overview of distinct expression profiles of mouse Enah splice variants during mouse development, in adult mouse tissues and in a subset of mouse cell lines. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3670886 Wed, 16 Jun 2010 23:00:00 +0100 3670886 http://www.medworm.com/index.php?rid=3670885&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F46 Conclusion: The alternative trans-splicing of sma-9 may contribute to the diversity of functions necessary to mediate tissue-specific outputs of BMP signaling. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3670885 Wed, 16 Jun 2010 23:00:00 +0100 3670885 http://www.medworm.com/index.php?rid=3665482&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F44 Conclusions: We demonstrate that the measure of individual miRNA by the bead-based method is feasible, high speed, sensitive and low cost. The Luminex xMAP technology also provides flexibility, since the central reaction can be scaled up with additional miRNA capturing beads, allowing validation of many differentially expressed miRNAs obtained from microarrays in a single experiment. We propose this technology as an alternative method to qRT-PCR for validating miRNAs expression data obtained with high-throughput technologies. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3665482 Tue, 15 Jun 2010 23:00:00 +0100 3665482 http://www.medworm.com/index.php?rid=3624845&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F43 Conclusions: Alternatively spliced RNA variants are prevalent in the human DHRS4 gene cluster. Based on the analysis of gene transcripts and bioinformatic prediction, we propose here that antisense transcription may be involved in the transcriptional initiation regulation of DHRS4 and in the posttranscriptional splicing of DHRS4L2 and DRHS4L1 for the homologous identity of DHRS4 gene cluster. Beside the alternative transcriptional start sites, the antisense RNA is novel possible factor serving to remove exon 1 from the transcripts initiated from exon a1 and exon a2. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3624845 Wed, 02 Jun 2010 23:00:00 +0100 3624845 http://www.medworm.com/index.php?rid=3617441&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F42 Conclusions: We conclude that pIREShyg2, which sensitizes its bicistronic transcripts to NMD, may be useful for studying NMD but should be avoided when maximum expressions of PTC-harboring genes are required. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3617441 Mon, 31 May 2010 23:00:00 +0100 3617441 http://www.medworm.com/index.php?rid=3609868&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F41 Background: Accurate amino acid insertion during peptide elongation requires tRNAs loaded by cognate amino acids and that anticodons match codons. However, tRNA misloading does not necessarily cause misinsertions: misinsertion is avoided when anticodons mismatch codons coding for misloaded amino acids. Presentation of the hypothesis: Occasional compensation of misacylation by codon-anticodon mismatch necessarily occurs. Putatively, occasional error compensation may be enhanced beyond the random combination of independent errors in tRNA loading and codon-anticodon interactions: tRNA misacylation might alter potentials for codon-anticodon mismatches, perhaps specifically increasing potentials for mismatching those codons coding for the misacylated non-cognate amino acid. This hypothetical ph... BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3609868 Thu, 27 May 2010 23:00:00 +0100 3609868 http://www.medworm.com/index.php?rid=3607036&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F40 Conclusion: The gene expression profiles of two archaeal Lrp-homologues report in detail their role inH. salinarum R1. LrpA1 and Lrp show similar functions to those already described in bacteria, but in addition they play a key role in regulatory networks, such as controlling the transcription of other regulators. In a more detailed analysis ligand dependent binding of LrpA1 was demonstrated to its target gene aspB3. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3607036 Thu, 27 May 2010 23:00:00 +0100 3607036 http://www.medworm.com/index.php?rid=3588560&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F39 Conclusions: We recommend ACTB and RPII as stable reference genes most suitable for gene expression studies of human visceral adipose tissue. The use of these genes as a reference pair may further enhance the robustness of qRT-PCR in this model system. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3588560 Thu, 20 May 2010 23:00:00 +0100 3588560 http://www.medworm.com/index.php?rid=3581136&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F38 Conclusions: We demonstrate that the GATA-1 transcription factor binds and transactivates the ETO proximal promoter in an erythroid/megakaryocytic-specific manner. Thus, trans-acting factors that are essential in erythroid/megakaryocytic differentiation govern ETO expression. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3581136 Wed, 19 May 2010 23:00:00 +0100 3581136 http://www.medworm.com/index.php?rid=3555539&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F37 Conclusion: Our findings provide valuable insights about genomic information and nutritional regulation of TGF-beta6 which will aid the further investigation of the S. aurata TGF-beta6 gene in association with muscle growth. The finding of a novel TGF-beta6 molecule, unique to fish, will contribute to the understanding of the evolution of the TGF-beta family of cytokines in vertebrates. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3555539 Tue, 11 May 2010 23:00:00 +0100 3555539 http://www.medworm.com/index.php?rid=3551719&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F36 Conclusion: Generally, it was found that EF1A1 and RPL7 were the genes that showed least variation, with HPRT1 and UbcE as intermediate genes, and ACTB1 and Tubb2C as the least stable ones. None of the six reference genes can be recommended as reference gene candidates in ConA-PMA stimulated leucocytes. However, UbcE can be a good candidate in other experimental setups. This study emphasizes the need for reference gene evaluation, as universal reference genes have not been identified. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3551719 Mon, 10 May 2010 23:00:00 +0100 3551719 http://www.medworm.com/index.php?rid=3548817&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F35 Conclusions: The demonstration that ZFN expression, in addition to stimulating specific gene targeting by HR, leads to increased rates of illegitimate integration emphasizes the importance of careful characterization of ZFN treated cells. In order to reduce off-target events, reversible cell cycle arrest of the target cells in the G2/M phase is an efficient way for increasing the ratio between specific HR and illegitimate integration. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3548817 Sun, 09 May 2010 23:00:00 +0100 3548817 http://www.medworm.com/index.php?rid=3518786&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F32 Conclusions: Our findings implicate novel, genome-based mechanisms as regulators of the terminal events in the mouse HH signaling cascade. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3518786 Thu, 29 Apr 2010 23:00:00 +0100 3518786 http://www.medworm.com/index.php?rid=3518787&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F31 Conclusion: These results indicate a unique role for Topors in the maintenance of genomic stability and pericentric heterochromatin, as well as in cellular sensitivity to histone deacetylase inhibitors. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3518787 Wed, 28 Apr 2010 23:00:00 +0100 3518787 http://www.medworm.com/index.php?rid=3514848&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F30 Conclusions: This method of qPCR analysis does not use any explicit or implicit measure of efficiency, and may therefore be immune to problems inherent in other qPCR approaches. It yields an estimate of absolute initial copy number of template, and controlled tests show it generates accurate results. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3514848 Wed, 28 Apr 2010 23:00:00 +0100 3514848 http://www.medworm.com/index.php?rid=3507618&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F29 Conclusions: MiRNA expression patterns differed significantly in response to host genetic components. Approximately 20% of the miRNAs in this study were identified as being correlated with backfat thickness. This result suggests that miRNAs may play a regulatory role in white adipose tissue development in beef animals. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3507618 Mon, 26 Apr 2010 23:00:00 +0100 3507618 http://www.medworm.com/index.php?rid=3498541&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F27 Conclusions: Our results indicate that eef1b and ubq are generally the most suitable reference genes for the conditions and tissues in the present epaulette shark studies. These genes could also be potential reference gene candidates for other physiological studies examining stress in elasmobranchs. The results emphasise the importance of inter-group variation in reference gene evaluation. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3498541 Thu, 22 Apr 2010 23:00:00 +0100 3498541 http://www.medworm.com/index.php?rid=3498540&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F28 Conclusions: This research provides new insights into the impacts that socio-environmental factors (JH, soldiers, primer pheromones) can have on termite gene expression and caste differentiation, and reveals a number of socially-relevant genes that will be investigated in subsequent caste differentiation research. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3498540 Thu, 22 Apr 2010 23:00:00 +0100 3498540 http://www.medworm.com/index.php?rid=3456408&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F26 Conclusions: The combination of beta-actin and glyceraldehyde-3-phosphate dehydrogenase appeared to be the most suitable reference for studying alterations in gene expression in peripheral blood mononuclear cells related to vulnerability and resilience to PTSD, as well as to trauma-provoked developing of this disorder and recovery from it. Using glyceraldehyde-3-phosphate dehydrogenase, beta-actin and beta2-microglobulin as individual endogenous controls would provide satisfactory data, while RNA polymerase II polypeptide A could not be recommended. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3456408 Thu, 08 Apr 2010 23:00:00 +0100 3456408 http://www.medworm.com/index.php?rid=3410870&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F23 Conclusions: Striking differences were found in the structure and developmental expression of the four hexamerin genes in the honey bee. The presence of a potential binding site for Usp in the respective 5' UCRs, and the results of experiments on JH level manipulation in vivo support the hypothesis of regulation by JH. Transcript levels and patterns in the fat body and gonads suggest that, in addition to their primary role in supplying amino acids for metamorphosis, hexamerins serve as storage proteins for gonad development, egg production, and to support foraging activity. A phylogenetic analysis including the four deduced hexamerins and related proteins revealed a complex pattern of evolution, with independent radiation in insect orders. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3410870 Fri, 26 Mar 2010 00:00:00 +0100 3410870 http://www.medworm.com/index.php?rid=3394578&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F22 Conclusions: The most stable reference genes were Rpl32, Gapdh and Polr2a in mouse post-infarction heart failure, Polr2a, Rpl32 and Tbp in rat post-infarction heart failure and Rpl32 and Pgk1 in human heart failure (ischemic disease and cardiomyopathy). The overall most stable reference genes across all three species was Rpl32 and Polr2a. In rat myocardium, all reference genes tested showed substantial variation with developmental stage, with Rpl4 as was most stable among the tested genes. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3394578 Tue, 23 Mar 2010 00:00:00 +0100 3394578 http://www.medworm.com/index.php?rid=3358375&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F21 Conclusion: The low activity of TFIIIB in PE can be attributed to a combination of changes, any one of which could be sufficient to inhibit pol III transcription. Declining levels of essential TFIIIB subunits and of activators that are required for maximal TFIIIB activity are accompanied by an increase in a potent repressor of TFIIIB. These events provide fail-safe guarantees to ensure that pol III output is appropriate to the diminished metabolic requirements of terminally differentiated cells. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3358375 Fri, 12 Mar 2010 00:00:00 +0100 3358375 http://www.medworm.com/index.php?rid=3345980&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F20 Conclusions: The lack of regulatory elements corresponding to human TSIX lends further support to the argument that TSIX is not a regulator of XIST in humans. The upstream hypersensitive sites we identify show sequence conservation with other eutheria, but not with mice. Therefore the regulation of XIST seems to be different between mice and man, and regulatory sequences upstream of XIST may be important regulators of XIST in non-rodent eutheria instead of Tsix which is critical for Xist regulation in rodents. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3345980 Mon, 08 Mar 2010 00:00:00 +0100 3345980 http://www.medworm.com/index.php?rid=3342007&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F19 Conclusions: Taken together, we have demonstrated that octamer-containing regions are associated with excretory cell expression of several genes that have putative roles in osmoregulation. Moreover, our analysis of the octamer sequence and its sequence variants could aid in the identification of additional genes that are expressed in the excretory cell and that may also be regulated by CEH-6. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3342007 Mon, 08 Mar 2010 00:00:00 +0100 3342007 http://www.medworm.com/index.php?rid=3337863&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F18 Conclusion: DNA-PKcs plays a dominant role in regulating the phosphorylation of H2AX in response to both DNA damage and cell cycle progression. It can directly phosphorylate H2AX independent of ATM and indirectly modulate the phosphorylation level of gamma-H2AX via the Akt/GSK3 beta signal pathway. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3337863 Sat, 06 Mar 2010 00:00:00 +0100 3337863 http://www.medworm.com/index.php?rid=3283049&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F17 Conclusions: The data obtained suggest that alteration of rpsO, rpsT and rpsB-tsf transcript levels observed under conditions of Hfq deficiency is not caused by the post-transcriptional events, such as mRNA destabilization or changes in translation control, and may rather result from changes in transcriptional activity. So far, how Hfq affects transcription remains unclear. We propose that one of the likely mechanisms of Hfq-mediated modulation of transcription might operate early in the elongation step, when interaction of Hfq with a nascent transcript would help to overcome transcription pauses and to prevent preliminary transcript release. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3283049 Thu, 18 Feb 2010 00:00:00 +0100 3283049 http://www.medworm.com/index.php?rid=3279468&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F16 Conclusions: The approach used provided us a quantitative representation of the PLMVd-derived siRNAs retrieved from infected peach trees. These siRNAs account for a relatively large proportion of the small non-coding RNAs. Surprisingly, the siRNAs from some regions of the PLMVd genome appear over-represented, although these regions are not necessarily forming sufficiently long double-stranded structures to satisfy Dicer-like criteria for substrate specificity. Importantly, this large library of siRNAs gave several hints as to the components of the involved silencing machinery. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3279468 Tue, 16 Feb 2010 00:00:00 +0100 3279468 http://www.medworm.com/index.php?rid=3275411&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F15 Conclusions: geNorm, NormFinder, and BestKeeper analyses indicated that ACT, EF and rRNA had the highest expression stability across leaf and root tissues, while GAPDH and NADHD showed relatively low expression stability. The results of this study emphasise the importance of validating reference genes for qRT-PCR analysis in chicory. The use of the most stable reference genes such as ACT and EF allows accurate normalisation of gene expression in chicory leaf and root tissues. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3275411 Mon, 15 Feb 2010 00:00:00 +0100 3275411 http://www.medworm.com/index.php?rid=3253955&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2Fbmcmolbiol%2F Conclusion: These studies provide, for the first time, the evidence that SLUG expression is correlated with osteogenic commitment, and is positively regulated by Lef-1 signal in normal human osteoblasts. These findings will help to further understand the regulation of the human SLUG gene and reveal the biological functions of SLUG in the context of bone tissue. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3253955 Wed, 03 Feb 2010 00:00:00 +0100 3253955 http://www.medworm.com/index.php?rid=3207836&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F11 Conclusions: NrdB is present in nucleoid-associated clusters during the replication period. These clusters disappear after replication ends. The number of these clusters is closely related to the number of replication forks and the number of three replication protein clusters in any cell cycle period. Therefore we conclude that NrdB protein, and most likely RNR protein, is closely linked to the replication proteins or replisome at the replication fork. These results clearly support the replication hyperstructure model. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3207836 Tue, 26 Jan 2010 00:00:00 +0100 3207836 http://www.medworm.com/index.php?rid=3200032&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F10 Conclusion: Together our data define a TAF6delta-driven apoptotic gene expression program and show crosstalk between the p53 and TAF6delta pathways. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3200032 Fri, 22 Jan 2010 00:00:00 +0100 3200032 http://www.medworm.com/index.php?rid=3193158&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F7 Conclusion: The panel of reference genes identified in the present study will enable more accurate normalisation of gene expression data in the acute phase of experimental ICH. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3193158 Wed, 20 Jan 2010 00:00:00 +0100 3193158 http://www.medworm.com/index.php?rid=3193157&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F8 Conclusions: This study is unique in the magnitude of samples tested with the inclusion of numerous field-grown samples, helping pave the way to conduct gene expression studies in perennial biomass crops under field-conditions. From our study several stably expressed reference genes have been validated. This provides useful candidates for reference gene selection in perennial ryegrass under conditions other than those tested here. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3193157 Wed, 20 Jan 2010 00:00:00 +0100 3193157 http://www.medworm.com/index.php?rid=3188606&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F6 Conclusions: This study has identified and characterised the expression profiles of members of the anhydrobiotic gene set in A. avenae. It also demonstrates the potential of RNAi for the analysis of anhydrobiosis and provides the first genetic data to underline the importance of effective antioxidant systems in metazoan desiccation tolerance. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3188606 Tue, 19 Jan 2010 00:00:00 +0100 3188606 http://www.medworm.com/index.php?rid=3181828&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F5 Conclusion: From these results we conclude that USF1 and USF2 are activators of the bovine placenta-specific promoter P1.1 and thus act in the opposite mode as in the case of the non-orthologous human placenta-specific promoter. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3181828 Mon, 18 Jan 2010 00:00:00 +0100 3181828 http://www.medworm.com/index.php?rid=3178447&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F4 Conclusions: Our approach revealed that small molecules derived from halogenated imidazole compounds may decrease cell proliferation, in part, by inhibiting pathways that regulate transcription elongation . (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3178447 Fri, 15 Jan 2010 00:00:00 +0100 3178447 http://www.medworm.com/index.php?rid=3175257&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F3 Conclusions: From these experimental studies we propose that TACC1 might be a scaffold protein building up a transcriptional complex around the NRs we studied. This function of TACC1 might account for its involvement in several forms of tumour development. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3175257 Fri, 15 Jan 2010 00:00:00 +0100 3175257 http://www.medworm.com/index.php?rid=3171304&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F11%2F2 Conclusions: Variant GLI binding sites with relatively low affinity can within natural promoters lead to strong transcriptional activation. This may facilitate the identification of additional direct GLI target genes. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3171304 Wed, 13 Jan 2010 00:00:00 +0100 3171304 http://www.medworm.com/index.php?rid=3133222&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F113 Conclusions: The need for an accurate and sensitive technique to quantify mRNA expression levels makes the described modified primer technology a promising tool for use in multiplex one-step RT-PCR. A more accurate representation of the abundances in initial template sample is feasible with modified primers, as artifacts of biased PCR are reduced because of greater improvements in reaction specificity. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3133222 Wed, 30 Dec 2009 00:00:00 +0100 3133222 http://www.medworm.com/index.php?rid=3128756&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F112 Conclusion: Based on these results, ELF1A can be proposed as an initial reference gene for normalization of quantitative RT-PCR data in whole R. microplus and R. appendiculatus ticks. The observed differences in Bm86 expression profile between the two species alone can not adequately explain the lack of a Bm86 vaccination effect in R. appendiculatus. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3128756 Tue, 29 Dec 2009 00:00:00 +0100 3128756 http://www.medworm.com/index.php?rid=3118414&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F111 Conclusion: We conclude that this SCOTS method has a key importance for the transcriptomic analysis of ER and can be potentially used for other Rickettsiales. This study constitutes the first step for further gene expression analyses that will lead to a better understanding of both ER pathogenicity and the adaptation of obligate intracellular bacteria to their environment. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3118414 Thu, 24 Dec 2009 00:00:00 +0100 3118414 http://www.medworm.com/index.php?rid=3106615&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F110 Conclusions: TLKs play important roles in DNA repair, not only by modulation of chromatin assembly via Asf1, but also by a more direct function in processing the ends of DSBs via interaction with Rad9. Inhibition of Rad9 phosphorylation in KD-overexpressing cells may have consequences in signaling completion of the repair and cell cycle re-entry, and could explain a loss of viability from DSBs in these cells. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3106615 Sun, 20 Dec 2009 00:00:00 +0100 3106615 http://www.medworm.com/index.php?rid=3145362&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2Fbmcmolbiol%2F Conclusion: Our findings suggest that NFI-A, in particular its brain-specific isoform, represses L1 gene expression, and might act as a second silencer of L1 in addition to the neural restrictive silencer factor (NRSF). (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3145362 Mon, 14 Dec 2009 00:00:00 +0100 3145362 http://www.medworm.com/index.php?rid=3088732&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F109 Conclusion: The level of CPEB3 was up-regulated in the retina during development. The presence of multiple CPEB3 isoforms indicates remarkable complexity in the regulation and function of CPEB3. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3088732 Mon, 14 Dec 2009 00:00:00 +0100 3088732 http://www.medworm.com/index.php?rid=3084432&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F107 Conclusion: Our findings suggest that NFI-A, in particular its brain-specific isoform, represses L1 gene expression, and might act as a second silencer of L1 in addition to the neural restrictive silencer factor (NRSF). (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3084432 Mon, 14 Dec 2009 00:00:00 +0100 3084432 http://www.medworm.com/index.php?rid=3084431&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F108 Conclusion: Episomal hobo/Hermes elements are common products of element excision and can be maternally transmitted. Episomal forms of Hermes are capable of integration and also of influencing the transposition of canonical elements suggesting biological roles for these extrachromosomal elements in element transmission and regulation. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3084431 Mon, 14 Dec 2009 00:00:00 +0100 3084431 http://www.medworm.com/index.php?rid=3082044&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F106 Conclusion: The reference gene expression stability varied considerably among the feline tissues investigated. No tested gene was optimal for normalisation in all tissues. For the majority of the tissues, two to three reference genes were necessary for accurate normalisation. The present study yields essential information on the correct choice of feline reference genes depending on the tissues analysed. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3082044 Fri, 11 Dec 2009 00:00:00 +0100 3082044 http://www.medworm.com/index.php?rid=3033335&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F105 Conclusions: Curves related to this symmetric distribution along the DNA sequence identify preferential sites for positioning of the dyad axis, which we termed palinstases. The comparison of our data with known nucleosome positions in archaeal and eukaryotic sequences shows many coincidences of location. Sequences that impair nucleosome formation and DNase I hypersensitive sites yield curves with a lower degree of symmetry. Analysis performed on DNA tracts of promoters close to the transcription start and termination sites identified peculiar patterns: in particular low affinity for nucleosome binding at the transcription start site and a high affinity exactly at the transcription termination site, suggesting a major role of nucleosomes in the termination of transcription. (Source: BMC Mol... BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3033335 Fri, 27 Nov 2009 00:00:00 +0100 3033335 http://www.medworm.com/index.php?rid=3029309&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F104 Conclusion: This is the first molecular identification of fish arsenite transport systems and we propose that the extensive expression of the fish aquaglyceroporins and their ability to transport metalloids suggests that aquaglyceroporins are the major pathways for arsenic accumulation in a variety of zebrafish tissues. Uptake is one important step of arsenic metabolism. Our results will contribute to a new understanding of aquatic arsenic metabolism and will support the use of zebrafish as a new model system to study arsenic associated human diseases. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3029309 Wed, 25 Nov 2009 00:00:00 +0100 3029309 http://www.medworm.com/index.php?rid=3003237&cid=s_34036_67_f&fid=34036&url=http%3A%2F%2Fwww.biomedcentral.com%2F1471-2199%2F10%2F103 Conclusion: This work establishes the identity of cis sequences that regulate alternative VEGFsplicing and dictate the relative expression levels of VEGF isoforms. (Source: BMC Molecular Biology - Latest articles) BMC Molecular Biology - Latest articles journals http://www.medworm.com/rss/comments.php?id=3003237 Tue, 17 Nov 2009 00:00:00 +0100 3003237