MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Bio-Medical Materials and Engineering' source. Fri, 30 Oct 2009 16:25:47 +0100 http://www.medworm.com/index.php?rid=2745936&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19714791%26dopt%3DAbstract Authors: PMID: 19714791 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2745936 Mon, 31 Aug 2009 06:56:03 +0100 2745936 http://www.medworm.com/index.php?rid=2581027&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581701%26dopt%3DAbstract Authors: Yada K, Abe T, Haga N Samples of Paramecium caudatum are observed by means of a scanning electron microscope (SEM) and a projection X-ray microscope (XRM) with computer tomography (CT) function. The samples are fixed with two kinds of fixatives, glutaraldehyde and osmium-tetra oxide acid. After the fixation and replacement procedure with t-buthyl alcohol, the samples followed by a freeze drying, well retain their structures. Surface structures, cilia and microfibrillar systems including infraciliary lattice structures, are clearly depicted by SEM observation. On the other hand, XRM images give quite different information, namely, in the case of osmium oxide fixation, the structures of internal organelles like the macronucleus placed in the central part of cell body and trichoc... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581027 Wed, 08 Jul 2009 20:04:03 +0100 2581027 http://www.medworm.com/index.php?rid=2581026&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581702%26dopt%3DAbstract Authors: Sakaguchi N, Watari F, Yokoyama A, Nodasaka Y, Ichinose H In the present study, we focused on the optimal conditions for observation of morphology and atomic structure of carbon nanotube (CNT) in vivo by transmission electron microscopy (TEM). Either low-voltage or high-voltage TEMs was chosen for the high-contrast or high-resolution imaging of subcutaneous tissue and the multi-wall CNT (MWCNT). The morphology and structure of each cell organelle were well recognized using the low-voltage TEM at 75 kV. Individual MWCNTs forming the cluster were also visible by the low-voltage TEM. On the contrary, the high-voltage TEM image at 1250 kV shows poor contrast on both the cell organelles and MWCNTs. However, graphene layers of MWCNT were clearly visible in the HRTEM image using the ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581026 Wed, 08 Jul 2009 20:04:03 +0100 2581026 http://www.medworm.com/index.php?rid=2581025&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581703%26dopt%3DAbstract Authors: Watanabe Y, Sato K, Yukumi S, Yoshida M, Yamamoto Y, Doi T, Sugishita H, Naohara T, Maehara T, Aono H, Kawachi K Magnetic metal particles are known to induce heat energy under an alternating magnetic field (AMF). We developed a local tumor-heating device incorporating an MgFe2O4 needle for the purpose of mild ablation for cancer treatment. A needle made from sintered MgFe2O4 particles was embedded in the hepatic or breast tumors. Tumors were then heated by the energy dissipated from the needle exposed to an AMF. We sequentially evaluated histological changes, cellular activity of tumors, and the extent of thermal effect using nicotinamide adenine dinucleotide (NADH) diaphorase and terminal deoxynucleotidyl tranferase-mediated digoxigenin-DUTP nick-end labeling (TUNEL) staining... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581025 Wed, 08 Jul 2009 20:04:03 +0100 2581025 http://www.medworm.com/index.php?rid=2581024&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581704%26dopt%3DAbstract Authors: Ueno S, Shimabayashi S Certain molecules, which are able to directly translocate across phospholipid bilayer membranes (cell or endosormal membrane), can be useful as carriers (vectors) for drags (especially polymeric drags). We have studied the translocationability of the hydroxyapatite nanoparticle-poly-L-arginine complex through the negatively charged phospholipid bilayer membranes by using several instruments. It was confirmed by means of a confocal laser scanning microscopy (CLSM) not only the fact that the complex can translocate through the membranes but also the fact that the complexes were still retained in the inner water layer of the liposome even after the translocation. PMID: 19581704 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581024 Wed, 08 Jul 2009 20:04:03 +0100 2581024 http://www.medworm.com/index.php?rid=2581023&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581705%26dopt%3DAbstract In this study, calcium nitrate solutions of various concentrations were used for the conversion of hopeite crystals into CP. The products after the solution treatment were analyzed by X-ray diffractometry (XRD), Fourier transform infrared spectrometry (FTIR), and scanning electron microscope (SEM) observation. These results indicated that the converted scholzite crystals could be partially detected. Several types of set zinc phosphate cement with different P/L ratios were arranged. The surface products of the set cement after the solution treatment were analyzed by XRD. However, the crystal phase such as hopeite was not detected except for zinc oxide. The set cement, which was treated with the calcium nitrate solution, was immersed in simulated body fluid (SBF). HAP-like crystals on the se... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581023 Wed, 08 Jul 2009 20:04:03 +0100 2581023 http://www.medworm.com/index.php?rid=2581022&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581706%26dopt%3DAbstract Authors: Kashiwazaki H, Kishiya Y, Matsuda A, Yamaguchi K, Iizuka T, Tanaka J, Inoue N We fabricated novel chitosan/hydroxyapatite (HAp) nanocomposites with porous structure by the co-precipitation and porogen leaching method. SEM observation confirmed that the porous chitosan/HAp nanocomposites with 60.6% and 87.1% porosity showed the interconnective pores with pore diameters in the range of 100-200 mum. The composites were found to be mechanically flexible and could be easily formed into any desired shape. The mechanical strength was enhanced by the heat treatment with saturated steam, which was ascribed to the formation of hydrogen bonds between chitosan molecules. The composites subcutaneously implanted in the backs of SD rats for 3 weeks caused little inflammation, and new blood v... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581022 Wed, 08 Jul 2009 20:04:03 +0100 2581022 http://www.medworm.com/index.php?rid=2581021&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581707%26dopt%3DAbstract Authors: Tazaki J, Murata M, Akazawa T, Yamamoto M, Ito K, Arisue M, Shibata T, Tabata Y The purpose of this study is to compare in vivo retention of BMP-2 and bone induction in HAp (porosity: 60-80%, pore size: 100-600 mum, sintering temperature: 800 degrees C, surface area: 1 m2/g) and beta-TCP (porosity: 75%, pore size: 100-400 mum, sintering temperature: 1050 degrees C, surface area: 4 m2/g). We estimated the in vivo release profile of 125I-labeled BMP-2 and bone induction of hard tissues histologically. The amount of BMP-2 remaining in the beta-TCP at 1 day after implantation was 49.6%, while the amount was 34.0% in the HAp. Furthermore, the HAp and beta-TCP containing 0.0, 0.05, 0.1, 0.3, 0.5, 1.0, 5.0 mug of BMP-2 were implanted into the back subcutis of 4-week old Wistar rats. ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581021 Wed, 08 Jul 2009 20:04:03 +0100 2581021 http://www.medworm.com/index.php?rid=2581020&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581708%26dopt%3DAbstract In this study, we investigated adhesion of osteoblast-like cells to single-walled carbon nanotube (SWNT) and multi-walled carbon nanotube (MWNT) sheets and compared these adhesions with that on a cell culture polystyrene dish by using a cell adhesion test and a scanning electron microscope. The MWNT sheets exhibited faster adhesion of cells at an initial stage than SWNT sheets and cell culture polystyrene dish. The number of attached cells on the MWNT sheets seemed to be greater than on SWNT sheets and cell culture polystyrene. Moreover, the MWNT sheets exhibited both high speed and good capacity for cell adhesion. However, the surface of the MWNT sheets was such that it facilitated cell adherence but hindered the spreading of the attached cells. Interestingly, cell adhesion to CNT sheets ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581020 Wed, 08 Jul 2009 20:04:03 +0100 2581020 http://www.medworm.com/index.php?rid=2581019&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581709%26dopt%3DAbstract In this study, a coating of carbon nanotubes (CNTs) was applied to silicone rubber to improve its cellular adhesiveness. Scanning electron micrograph of this coating revealed that CNTs had formed a densely packed meshwork; the Ra values and protein adsorption capacity were enhanced. Although the contact angle did not change after coating, it decreased after immersion into a culture medium. After cultivation for 6 d, while Saos-2 cells were hardly observed on untreated silicone, the cells proliferated on CNT-coated silicone. Thus, CNT coating might be a simple and effective solution to problems associated with silicone implants. PMID: 19581709 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581019 Wed, 08 Jul 2009 20:04:03 +0100 2581019 http://www.medworm.com/index.php?rid=2581018&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581710%26dopt%3DAbstract Authors: Nikaido T, Weerasinghe DD, Waidyasekera K, Inoue G, Foxton RM, Tagami J An acid-base resistant zone (ABRZ) has been shown to be created under a hybrid layer in a self-etching adhesive system at the adhesive/dentin interface. The purpose of this study was to assess the nanostructure of the ABRZ by applying all-in-one adhesive systems. Human premolar dentin was treated with one of two all-in-one adhesive systems; Clearfil Tri-S Bond and G-Bond according to the manufacturers' instructions. After placement of a resin composite, the bonded interface was vertically sectioned and subjected to an acid-base challenge. Following this, the nanostructure of the ABRZ was examined by SEM and TEM. The SEM observations of the adhesive-dentin interface after the acid-base challenge indicated t... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581018 Wed, 08 Jul 2009 20:04:03 +0100 2581018 http://www.medworm.com/index.php?rid=2581017&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581711%26dopt%3DAbstract Authors: Endo K, Ohno H Finely powdered enamel was used to develop a prosthetic resin composite that has good mechanical properties and no potential to abrade opposing tooth structure. Bovine teeth were ground into powder and then the enamel particles were separated from the powder by centrifugation in bromoform-ethanol solution. The resin matrix consisted of UDMA (60 mole %) and Tri-EDMA (40 mole %). Camphorquinone (0.5 mass %) was added to the monomer as a photo-initiator. Fillers were incorporated directly into the resin matrix in amounts of 80 or 85 mass %. The flexural strength and Vickers hardness (Hv) were measured. The average flexural strength and Hv values for specimens having 85 mass % filler that had been subjected to heat treatment at 100 degrees C after light-curing were ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581017 Wed, 08 Jul 2009 20:04:03 +0100 2581017 http://www.medworm.com/index.php?rid=2581016&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581712%26dopt%3DAbstract In this study, we investigated the changes induced in the surfaces of tooth slices by the application of a coating of CNTs by observing CNT-coated tooth slices both macroscopically as well as under a scanning electron microscope. Further, we investigated the effect of CNT coating on the tensile bond strength of dentin adhesives. CNTs adhered easily to the tooth surfaces when tooth slices were suspended in a CNT-dispersed solution. Interestingly, it was observed that CNTs selectively adhered to the surfaces of dentin and cementum, possibly by adhering to their exposed collagen fibers. In addition, the CNT coating did not affect the tensile bond strength of dentin adhesives. These results indicate that coating of the teeth with CNTs can be a possible application of CNTs as dental materials. ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581016 Wed, 08 Jul 2009 20:04:03 +0100 2581016 http://www.medworm.com/index.php?rid=2581015&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581713%26dopt%3DAbstract Authors: Takada T, Furusaki A, Tanaka Y The volatile organic compound (VOC) reduction activity of scallop shell powders fired at 300, 600 and 900 degrees C was examined using formaldehyde (HCHO). Raw shells as well as fired shells immediately after firing at several temperatures, except for 600 degrees C, were found to gradually remove HCHO from the air. In the case of shell powders stored for 3 months after firing, the HCHO reduction activity of the powder fired at 900 degrees C was obviously improved, with the HCHO concentrations rapidly reaching zero within 20 min. It has been found by X-ray diffraction measurements that shell powder stored for 3 months after firing at 900 degrees C contains a small amount of calcium hydroxide (Ca(OH)2) generated from calcium oxide (CaO). Our result... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581015 Wed, 08 Jul 2009 20:04:03 +0100 2581015 http://www.medworm.com/index.php?rid=2581014&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581714%26dopt%3DAbstract Authors: Sultana R, Hamada K, Ichikawa T, Asaoka K The purpose of this study was to determine the effects of heat treatment on the bioactivity of hydrothermal-modified titanium in CaO solution for improved bioactivity by immersion in simulated body fluid (SBF). The hydrothermal treatment of titanium in CaO solution was performed at 121 degrees C at 0.2 MPa for 1 h in an autoclave followed by 1 h heat treatments at 200, 400, 600 and 800 degrees C simultaneously. The bioactivity of titanium was evaluated by hydroxyapatite precipitation during immersion in SBF. Surface microstructure changes after the heat treatments and immersion in SBF were determined by X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM). Heat treatments at high te... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581014 Wed, 08 Jul 2009 20:04:03 +0100 2581014 http://www.medworm.com/index.php?rid=2581013&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581715%26dopt%3DAbstract Authors: Takeuchi A, Munar ML, Wakae H, Maruta M, Matsuya S, Tsuru K, Ishikawa K The effect of temperature on crystallinity of carbonate apatite (CAp) foam prepared from alpha-tricalcium phosphate (alpha-TCP) foam by hydrothermal treatment was investigated in the present study. The alpha-TCP foams were prepared through a conventional sintering method using polyurethane foam as template. Then, the resultant alpha-TCP foams were hydrothermally treated with Na2CO3 aqueous solution at 100 degrees C, 150 degrees C and 200 degrees C for 72 h. After hydrothermal treatment, the cancellous bone-like macroporous structure of the alpha-TCP foams was maintained. However, microscopic morphology of the foams' frame significantly changed after the 72 h treatment period. The smooth surface of alpha-TC... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581013 Wed, 08 Jul 2009 20:04:03 +0100 2581013 http://www.medworm.com/index.php?rid=2581012&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581716%26dopt%3DAbstract In this study, we visualized the distribution of administered magnetic nanoparticles in mice using both X-ray scanning analytical microscopy (XSAM) and magnetic resonance imaging (MRI). After administration, the nanoparticles were rapidly dispersed via the blood circulation, and reached the liver, kidney and spleen. Using the XSAM and MRI methods in a complementary fashion, the biodistribution of nano-sized magnetite particles was successfully visualized. PMID: 19581716 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581012 Wed, 08 Jul 2009 20:04:03 +0100 2581012 http://www.medworm.com/index.php?rid=2581011&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581717%26dopt%3DAbstract Authors: Abe S, Koyama C, Esaki M, Akasaka T, Uo M, Kuboki Y, Morita M, Watari F We observed the internal diffusion behavior of inorganic micro/nano particles through oral administration. By oral exposure, the fed particles were absorbed through the digestive system then reached some organs after internal diffusion in the body. For example, TiO2 particles fed to mice were detected in the lung, liver, and spleen after 10 days of feeding. Whereas, the absorption efficiency was extremely low compared with intravenous injection. In a comparison of the simple amount of administration, oral exposure required 102 times or more amount by intravenous injection for detection by an X-ray scanning analytical microscope. During dental treatment, micro/nano particles from tooth or dental materials w... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581011 Wed, 08 Jul 2009 20:04:03 +0100 2581011 http://www.medworm.com/index.php?rid=2581010&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581718%26dopt%3DAbstract Authors: Yada K, Kohyama N Asbestos minerals are thin fiber type of minerals and honorably said as "the minerals of the miracle" because of their valuable natures even in the strategic field. On the other hand, the relation between asbestos exposure and diseases such as lung cancer and malignant mesothelioma was proved around 1970 by epidemiology and an animal experiment in relation to their microstructures. Here, microstructures of chrysotile asbestos, a mainstream of asbestos substances, are shown. It is also shown that in what kinds of environment people are exposed to asbestos and what kinds of biological or epidemical things happen after asbestos exposure. Many kinds of fibrous materials as the substitutes of asbestos are described in relation to their carcinogenicity. PMID: 1... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581010 Wed, 08 Jul 2009 20:04:03 +0100 2581010 http://www.medworm.com/index.php?rid=2581009&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581719%26dopt%3DAbstract Authors: Kovtun A, Heumann R, Epple M Transfection is a widely used method in molecular biology for the introduction of foreign nucleic acids (DNA or RNA) into eukaryotic cells that permits to control intracellular processes, i.e. the induction or inhibition of protein expression. Nucleic acids alone cannot penetrate the cell membrane, therefore special carriers like cationic polymers or inorganic nanoparticles are required. Single-shell and multi-shell calcium phosphate nanoparticles were prepared and functionalized with DNA and siRNA. Thereby, the expression of enhanced green fluorescing protein (EGFP) can be induced (by using pcDNA3-EGFP) or silenced (by using siRNA). The single-shell nanoparticles were prepared by rapid mixing of aqueous solutions of calcium nitrate and diammonium ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581009 Wed, 08 Jul 2009 20:04:03 +0100 2581009 http://www.medworm.com/index.php?rid=2581008&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581720%26dopt%3DAbstract Authors: Nagano F, Selimovic D, Noda M, Ikeda T, Tanaka T, Miyamoto Y, Koshiro K, Sano H Since adhesive technology was introduced into dental field, metal-based restoration has been gradually replaced by metal-free restoration. Using the adhesive technology, minimum invasive technique has been possible in daily clinical practice as well as esthetic tooth-colored restorations have become very popular all over the world.One of the current issues of the dental adhesive is durability of bond between tooth structure and adhesive resin. Several approaches to overcome the issues have been carried out. Self-etching approach is believed to create durable bond because demineralization of superficial tooth surface is very shallow. Other approach is to utilize the inhibitor of enzymes which are su... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581008 Wed, 08 Jul 2009 20:04:03 +0100 2581008 http://www.medworm.com/index.php?rid=2581007&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19581721%26dopt%3DAbstract Authors: Matsuda M, Hunt G We initiate some comparisons between Japan, Europe and USA on how far there is governmental support for the ethical, legal, social and environmental dimensions of nanotechnology development. It is evident that in the USA and Europe nanotechnology is now firmly embedded in the consideration of ELSI. Yet Japan has not yet adequately recognized the importance of these dimensions. The history of bioethics in Japan is short. In Europe, as early as 2004, a nanotechnology report by the UK's Royal Society referred to the possibility of some nanotubes and fibres having asbestos-like toxicity. The negative history of asbestos in Europe and USA is not yet fully identified as a Japanese problem. Japan is therefore in the process of seeking how best to address societal as... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2581007 Wed, 08 Jul 2009 20:04:03 +0100 2581007 http://www.medworm.com/index.php?rid=2527618&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458439%26dopt%3DAbstract Authors: PMID: 19458439 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527618 Sat, 27 Jun 2009 12:05:03 +0100 2527618 http://www.medworm.com/index.php?rid=2527617&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458440%26dopt%3DAbstract Authors: Kuboki Y, Terada M, Kitagawa Y, Abe S, Uo M, Watari F The interactions between carbon nanotubes and important biomolecules, above all collagen molecules, have not been studied in detail. This situation is partly due to the fact that CNT are solid entities, while most of the biomolecules can be prepared in solution. We used turbidity as a means of evaluating the interaction between CNT and collagen molecules. To a stable suspension of CNT (10 ppm in 0.1% Triton), collagen solution was added to obtain a final concentration of 25 ppm. The degree of aggregation was evaluated by measuring the turbidity of the suspension at 660 nm. It was found that native collagen induced distinct aggregation with CNT, while denaturation of this protein at 60 degrees C for 1 hr deprived the molecul... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527617 Sat, 27 Jun 2009 12:05:03 +0100 2527617 http://www.medworm.com/index.php?rid=2527616&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458441%26dopt%3DAbstract Authors: Uo M, Hasegawa T, Akasaka T, Tanaka I, Munekane F, Omori M, Kimura H, Nakatomi R, Soga K, Kogo Y, Watari F Three types of multiwalled carbon nanotube (MWCNT) monoliths without any binders were obtained by spark plasma sintering (SPS) treatment at 2000 degrees C under 80 MPa sintering pressure. Three MWCNTs with different diameters: thin (slashed circle20-30 nm, CNT Co., Ltd., Korea), thick (slashed circle100 nm, Nano Carbon Technologies Co., Ltd., Japan) and spherical thin (slashed circle20-30 nm, granulated diameter = 1-3 microm, Shimizu Corporation, Japan) were employed for SPS. SEM observation confirmed that these materials maintained the nanosized tube microstructure of raw CNT powder after SPS treatment. The densest monolith was prepared with the spherical MWCNTs. The mec... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527616 Sat, 27 Jun 2009 12:05:03 +0100 2527616 http://www.medworm.com/index.php?rid=2527615&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458442%26dopt%3DAbstract Authors: Matsuoka A, Onfelt A, Matsuda Y, Nakaoka R, Haishima Y, Yudasaka M, Iijima S, Tsuchiya T To evaluate the role of particle size in cytotoxicity tests of nanomaterials (NMs), we exposed Chinese hamster cells to polystyrene (PS) spheres with defined diameters ranging from 0.1 to 9.2 microm. We found that the 4.45-microm PS particles were most cytotoxic while sizes 0.1 and 0.2 microm showed no cytotoxicity up to 1000 microg/ml. In the chromosome aberration test, the 4.45-microm PS particles induced polyploidy in a mass concentration-dependent manner in 24- and 48-h treatments. The 5.26-microm PS particles induced polyploidy only at 1000 microg/ml for 48 h. Next, we performed the cytotoxicity test with as-grown single walled carbon nanohorns (NHas). These were suspended in DMSO and... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527615 Sat, 27 Jun 2009 12:05:03 +0100 2527615 http://www.medworm.com/index.php?rid=2527614&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458443%26dopt%3DAbstract Authors: Yonezawa T In-situ TEM (transmission electron microscopy) of silver nanoparticle ink at high temperature was carried out. Into a carbon film tube coating the spiral tungsten filament which was attached to the TEM specimen holder, a silver nanoparticle ink was injected. After evaporation, silver nanoparticles with an aggregated structure could be observed. The temperature of the tungsten wire can be controlled by DC current supplied by dry batteries. Fusion of smaller nanoparticles was observed at a higher temperature and some particles were incorporated to larger particles. This technique can be generally used to consider the sintering temperature of such metal nanoparticles. PMID: 19458443 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527614 Sat, 27 Jun 2009 12:05:03 +0100 2527614 http://www.medworm.com/index.php?rid=2527613&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458444%26dopt%3DAbstract Authors: Koehne JE, Chen H, Cassell A, Liu GY, Li J, Meyyappan M Arrays of Carbon nanofibers (CNFs) harness the advantages of individual CNF as well the collective property of assemblies, which made them promising materials in biosensing and tissue engineering or implantation. Here, we report two studies to explore the applications of vertically aligned CNFs. First, a nanoelectrode array (NEA) based on vertically aligned CNFs embedded in SiO(2) is used for ultrasensitive DNA detection. Oligonucleotide probes are selectively functionalized at the open ends of the CNFs and specifically hybridized with oligonucleotide targets. The guanine groups are employed as the signal moieties in the electrochemical measurements. Ru(bpy)(3)(2+) mediator is used to further amplify the guanine oxidation... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527613 Sat, 27 Jun 2009 12:05:03 +0100 2527613 http://www.medworm.com/index.php?rid=2527612&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458445%26dopt%3DAbstract Authors: Terada M, Abe S, Akasaka T, Uo M, Kitagawa Y, Watari F Carbon nanotubes (CNTs) have excellent chemical durability, mechanical strength and electrical properties. Therefore, there is interest in CNTs for not only electrical and mechanical applications, but also biological and medical applications. We coated titanium, a common material for dental implants, with multiwalled carbon nanotubes (MWCNTs). First, titanium was aminated and covered with collagen. Then, the carboxylated MWCNTs were coated onto the collagen attached to the titanium plate. The collagen-coated titanium plate had a homogeneous MWCNT coating, which showed strong attachment to the titanium surface as a thin layer. The surface roughness was significantly increased with the MWCNT coating. MC3T3-E1 cells were cult... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527612 Sat, 27 Jun 2009 12:05:03 +0100 2527612 http://www.medworm.com/index.php?rid=2527611&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458446%26dopt%3DAbstract This study indicates that dentists and dental researchers should be concerned about the allergenic potential of dental metal materials. PMID: 19458446 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527611 Sat, 27 Jun 2009 12:05:03 +0100 2527611 http://www.medworm.com/index.php?rid=2527610&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458447%26dopt%3DAbstract Authors: Taira M, Nezu T, Sasaki M, Kimura S, Kagiya T, Harada H, Narushima T, Araki Y The purpose of this study was to examine gene expressions of macrophage phagocytizing sub-micro Ti particles by a DNA chip. Human monocytic cell line THP-1 was differentiated into macrophages by culturing for two days in medium supplemented with 200 nM phorbol ester (PMA). The macrophages were then cultured in four media: medium without PMA (control); medium with suspended sub-micro Ti particles (0.5 wt%); medium with 1.0 microg/ml lipopolysaccharide (LPS); and medium with LPS and Ti particles. After 6 hours' culture, total RNA were extracted and gene expressions were evaluated by DNA allergy chip with 205 allergy and inflammation related gene spots. We found that phagocytosis of sub-micro Ti particl... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527610 Sat, 27 Jun 2009 12:05:03 +0100 2527610 http://www.medworm.com/index.php?rid=2527609&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458448%26dopt%3DAbstract Authors: Baroth S, Bourges X, Goyenvalle E, Aguado E, Daculsi G A new biphasic calcium phosphate ceramic material has been developed in our laboratory. It is composed of 60% of hydroxyapatite and 40% of beta-tricalcium phosphate, based on three granulometries (submicron, round microporous 80-200 mum and macro microporous 0.5-1 mm particles) and hydrated with water leading the formation of a putty filler for bone repair. Biocompatibility and osteogenicity were tested by filling femoral epiphyses critical size bone defect and lumbar muscles in rabbit. After 3, 6 and 12 weeks of implantation, explants were treated for histology. Results revealed the biocompatibility of the material and intensive resorption of the submicron particle fraction followed by important bone ingrowth whereas oste... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527609 Sat, 27 Jun 2009 12:05:03 +0100 2527609 http://www.medworm.com/index.php?rid=2527608&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19458449%26dopt%3DAbstract Authors: Neupane MP, Park IS, Lee MH, Bae TS, Watari F TiO(2) nanotube array (TN) on titanium plate was fabricated by using an electrochemical method. The crystal structure and surface morphology of TN array was examined by X-ray diffraction (XRD) and Field Emission Scanning Electronic Microscopy (FE-SEM), respectively. The stability of the nanotube structure and crystal phase transition was studied at different temperatures in dry oxygen ambient. The as-deposited films were found to be amorphous. The tubes crystallized in the anatase phase at a temperature of 450 degrees C. Anatase crystallites formed inside the tubes walls was transformed completely to rutile at 500 degrees C in dry environment. With the heating temperature increased the intensity of rutile peak increased with decrea... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2527608 Sat, 27 Jun 2009 12:05:03 +0100 2527608 http://www.medworm.com/index.php?rid=2029337&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065017%26dopt%3DAbstract Authors: Sensebé L, Bourin P PMID: 19065017 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029337 Thu, 11 Dec 2008 18:04:41 +0100 2029337 http://www.medworm.com/index.php?rid=2029336&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065018%26dopt%3DAbstract Authors: McDevitt TC, Carpenedo RL, Sargent CY, Berguig GY, Marklein RA, Seaman S PMID: 19065018 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029336 Thu, 11 Dec 2008 18:04:41 +0100 2029336 http://www.medworm.com/index.php?rid=2029335&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065019%26dopt%3DAbstract Authors: Montemurro T, Bossolasco P, Cova L, Zangrossi S, Calzarossa C, Buiatiotis S, Soligo D, Silani V, Lambertenghi Deliliers G, Rebulla P, Lazzari L PMID: 19065019 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029335 Thu, 11 Dec 2008 18:04:41 +0100 2029335 http://www.medworm.com/index.php?rid=2029334&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065020%26dopt%3DAbstract Conclusion: This experimental study showed that adipose tissue engineering is feasible and gives comparable results to fat grafting. It allows a better understanding of the sequence of events following the transfer of adipose tissue. It provides not only volume but also undeniable stimulation, leading to significant thickening of the extracellular matrix. PMID: 19065020 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029334 Thu, 11 Dec 2008 18:04:41 +0100 2029334 http://www.medworm.com/index.php?rid=2029333&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065021%26dopt%3DAbstract Authors: Mojallal A, Auxenfans C, Lequeux C, Braye F, Damour O Adipose tissue is the standard autologous filling material used in plastic surgery today. At the same time it is also a source of mesenchymal stem cells, situated in the Stromal-Vascular Fraction (SVF) and easy to obtain in large quantities. The method of harvesting adipose tissue is an important stage for cell survival. So far, comparative studies on harvesting techniques have only concerned MTT cell viability of mature adipocytes. The aim of our study was to determine the influence of pressure on the yield of SVF cells in relation to the syringe aspiration technique which is the standard technique in plastic surgery. For this, six different harvesting conditions were tested on 3 patients. For each condition, a sample was ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029333 Thu, 11 Dec 2008 18:04:41 +0100 2029333 http://www.medworm.com/index.php?rid=2029332&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065022%26dopt%3DAbstract In conclusion, PAH-(PSS-PAH)(3) polyelectrolyte multilayer film seems to constitute an excellent material for MSC seeding. PMID: 19065022 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029332 Thu, 11 Dec 2008 18:04:41 +0100 2029332 http://www.medworm.com/index.php?rid=2029331&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065023%26dopt%3DAbstract Authors: Zhang L, Tran N, Chen HQ, Wang X Recent studies have shown effects of mechanical environment on bone marrow mesenchymal stem cells (BMSC). In order to examine how BMSC and their cytoskeleton respond to mechanical stimulation, we investigated their collagen synthesis and F-actin expression. Rat BMSC were harvested from adult rats and cultured to passage 4. Then the cells were seeded onto a silicone membrane loaded with an uniaxial cyclic stretching (10%, 1 Hz) during 3, 6, 12, 24 and 36 h. The levels of collagen type I and III before and after stretching were analyzed by immunocytochemistry, and the F-actin in cytoplasm was observed by confocal microscopy. Immunocytochemistry results showed that the stretching enhanced the synthesis of collagen types I and III in BMSC after 24 ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029331 Thu, 11 Dec 2008 18:04:41 +0100 2029331 http://www.medworm.com/index.php?rid=2029330&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065024%26dopt%3DAbstract Authors: Huselstein C, Netter P, de Isla N, Wang Y, Gillet P, Decot V, Muller S, Bensoussan D, Stoltz JF PMID: 19065024 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029330 Thu, 11 Dec 2008 18:04:41 +0100 2029330 http://www.medworm.com/index.php?rid=2029329&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065025%26dopt%3DAbstract Authors: Sanchez C, Gabay O, Henrotin YE, Berenbaum F PMID: 19065025 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029329 Thu, 11 Dec 2008 18:04:41 +0100 2029329 http://www.medworm.com/index.php?rid=2029328&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065026%26dopt%3DAbstract Authors: Tiitu V, Pulkkinen HJ, Valonen P, Kiviranta O, Kiekara T, Kiviranta I, Lammi MJ PMID: 19065026 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029328 Thu, 11 Dec 2008 18:04:41 +0100 2029328 http://www.medworm.com/index.php?rid=2029327&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065027%26dopt%3DAbstract We report here that it is possible (i) to build an alginate gel by alternate spraying of alginate and Ca(2+); (ii) to spray active alginate gel and cells; (iii) to build layer-by-layer an active reservoir under and on the top of this sprayed gel and cells; (iv) to follow the activity of these sprayed cells with time; (v) to propose a three-dimensional sprayed structure for tissue engineering application. PMID: 19065027 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029327 Thu, 11 Dec 2008 18:04:41 +0100 2029327 http://www.medworm.com/index.php?rid=2029326&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065028%26dopt%3DAbstract Authors: Hélary G, Poussard L, Zreiqat H, Migonney V PMID: 19065028 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029326 Thu, 11 Dec 2008 18:04:41 +0100 2029326 http://www.medworm.com/index.php?rid=2029325&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065029%26dopt%3DAbstract Authors: Peister A, Porter BD, Kolambkar YM, Hutmacher DW, Guldberg RE PMID: 19065029 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029325 Thu, 11 Dec 2008 18:04:41 +0100 2029325 http://www.medworm.com/index.php?rid=2029324&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065030%26dopt%3DAbstract Conclusion: the image quality obtained at 7 Teslas with our dedicated coil allowed segmentation of the cartilage compartments with good reproducibility. This study demonstrated that MRI is a useful technology to provide a non-invasive and reliable assessment of rat knee cartilage volume. PMID: 19065030 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029324 Thu, 11 Dec 2008 18:04:41 +0100 2029324 http://www.medworm.com/index.php?rid=2029323&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065031%26dopt%3DAbstract Conclusions: Intra-articular induction of Hsp70 by MG132 could be a safe and interesting tool in chondrocytes protection from cellular injuries and thus might be a novel chondroprotective modality in rat OA. PMID: 19065031 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029323 Thu, 11 Dec 2008 18:04:41 +0100 2029323 http://www.medworm.com/index.php?rid=2029322&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065032%26dopt%3DAbstract Authors: Watrin-Pinzano A, Loeuille D, Goebel JC, Lapicque F, Walter F, Robert P, Netter P, Corot C, Gillet P, Blum A The purpose of this study was to assess 2 Gd-based macromolecular intravascular contrast agents (P792, rapid clearance blood pool agent (rBPA) and P717, slow clearance blood pool agent (sBPA)) compared to Gd-DOTA (representative extracellular non specific agent) in MR imaging of knee rabbit experimental synovitis. Quantitative dynamic contrast enhanced MRI (qDCE-MRI) after intravascular injection of a low molecular weight contrast agent of 0.56 kDa (Gd-DOTA) and 2 high-molecular-weight contrast agents of 6.47 kDa (P792) and 52 kDa (P717) was performed in rabbits with carrageenan-induced synovitis of the right knee. P792 and P717 provided a progressive and persistent enh... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029322 Thu, 11 Dec 2008 18:04:41 +0100 2029322 http://www.medworm.com/index.php?rid=2029321&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065033%26dopt%3DAbstract Authors: Moretti M, Freed LE, Padera RF, Laganà K, Boschetti F, Raimondi MT PMID: 19065033 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029321 Thu, 11 Dec 2008 18:04:41 +0100 2029321 http://www.medworm.com/index.php?rid=2029320&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065034%26dopt%3DAbstract Authors: Das R, Kreukniet M, Oostra J, van Osch G, Weinans H, Jahr H PMID: 19065034 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029320 Thu, 11 Dec 2008 18:04:41 +0100 2029320 http://www.medworm.com/index.php?rid=2029319&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065036%26dopt%3DAbstract Authors: Crouchley CM, Barron V, Punchard M, O'Cearbhaill E, Smith T PMID: 19065036 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029319 Thu, 11 Dec 2008 18:04:41 +0100 2029319 http://www.medworm.com/index.php?rid=2029318&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065037%26dopt%3DAbstract Authors: Paternotte E, Gaucher C, Labrude P, Stoltz JF, Menu P Hypoxia is a diminution of oxygen quantity delivered to tissue for cellular need to product energy. Hypoxia derives from two major conditions in health diseases: anemia and ischemia. Anemic hypoxia comes from damage to O(2) transport like red blood cells diminution or disease. Ischemic hypoxia is a diminution of blood flow following a diminution of blood volume after a hemorrhagic shock. After hypoxia, vessels dilate to increase blood flow allowing a better oxygenation of peripheral tissues. This vasodilation appears immediately after the beginning of hypoxia and can be maintained during several hours. Today, the molecular mechanisms of this vasodilation stay unclear. But it seems that potassic channels, ATP concentration a... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029318 Thu, 11 Dec 2008 18:04:41 +0100 2029318 http://www.medworm.com/index.php?rid=2029317&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065038%26dopt%3DAbstract Authors: O'Brien T PMID: 19065038 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029317 Thu, 11 Dec 2008 18:04:41 +0100 2029317 http://www.medworm.com/index.php?rid=2029316&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065039%26dopt%3DAbstract Authors: Kadi A, Moby V, de Isla N, Lacolley P, Menu P, Stoltz JF PMID: 19065039 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029316 Thu, 11 Dec 2008 18:04:41 +0100 2029316 http://www.medworm.com/index.php?rid=2029315&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065040%26dopt%3DAbstract Authors: Shapira K, Dikovsky D, Habib M, Gepstein L, Seliktar D PMID: 19065040 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029315 Thu, 11 Dec 2008 18:04:41 +0100 2029315 http://www.medworm.com/index.php?rid=2029314&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065041%26dopt%3DAbstract Authors: Hornebeck W PMID: 19065041 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029314 Thu, 11 Dec 2008 18:04:41 +0100 2029314 http://www.medworm.com/index.php?rid=2029313&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065042%26dopt%3DAbstract Authors: Silva JN, Filipe P, Morlière P, Mazière JC, Freitas JP, Gomes MM, Santus R Photodynamic therapy (PDT) of skin tumors or pre-cancerous lesions and of age-related macular degeneration combines the administration of porphyrins or porphyrin precursors and illumination with red light at the diseased sites. Photosensitizers absorbing light beyond 630 nm where tissues have the highest transmittance produce singlet oxygen, a highly reactive activated oxygen species and a major cytotoxin. The PDT of age-related macular degeneration is performed with red laser light after i.v. injection of verteporfin (Visudyne((R))) a hydrophobic porphyrin carried by serum lipoproteins whose endocytosis leads to accumulation of the porphyrin in endothelial cells of choroidal neo-vessels. In t... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029313 Thu, 11 Dec 2008 18:04:41 +0100 2029313 http://www.medworm.com/index.php?rid=2029312&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065043%26dopt%3DAbstract Authors: Werkmeister E, Dumas D, de Isla N, Marchal L, Stoltz JF PMID: 19065043 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029312 Thu, 11 Dec 2008 18:04:41 +0100 2029312 http://www.medworm.com/index.php?rid=2029311&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065044%26dopt%3DAbstract Authors: Lu J, Laudinet J, Williams S PMID: 19065044 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029311 Thu, 11 Dec 2008 18:04:41 +0100 2029311 http://www.medworm.com/index.php?rid=2029310&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19065045%26dopt%3DAbstract Authors: Amouroux M, Blondel WC, Granel-Brocard F, Marchal F, Guillemin F PMID: 19065045 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=2029310 Thu, 11 Dec 2008 18:04:41 +0100 2029310 http://www.medworm.com/index.php?rid=1744249&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18725691%26dopt%3DAbstract The objective of the present study is to investigate the peak forces for a tracheal smooth muscle tissue subjected to an applied longitudinal vibration following isotonic shortening. A non-linear finite element analysis was carried out to simulate the vibratory response under experimental conditions that corresponds to forced length oscillations at 33 Hz for 1 second. The stiffness change and hysteresis estimated from the experimental data was used in the analysis. The finite element results of peak forces are compared to the experimental data obtained. The comparison of results indicate that the approach and the vibratory response obtained may be useful for describing the cross-bridge de-attachements within the cells as well as connective tissue connections characteristic of tracheal smoo... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1744249 Sat, 30 Aug 2008 15:06:19 +0100 1744249 http://www.medworm.com/index.php?rid=1744248&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18725692%26dopt%3DAbstract Authors: Saşmazel HT, Gümüşderelioğlu M, Gürpınar A, Onur MA In this contribution, PCL (poly-epsilon caprolactone) scaffolds were prepared by solvent-casting/particle-leaching technique in the presence of two pore formers, PEG(4000) or sucrose molecules in different quantities (0, 10, 20, 30, 40, 50, 55 w/w% PEG(4000)/PCL; 10, 20 w/w% Sucrose/PCL). The surface and bulk properties of the resulting scaffolds were studied by SEM, DSC and FTIR. SEM photographs showed that, macroporosity was obtained in the PCL structures prepared with sucrose crystals while microporous structure was obtained in the presence of PEG(4000) molecules. Average pore diameters calculated from SEM photographs were 40.1 and 191.2 mum for 40% PEG(4000)/PCL and 10% Sucrose/PCL s... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1744248 Sat, 30 Aug 2008 15:06:19 +0100 1744248 http://www.medworm.com/index.php?rid=1744247&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18725693%26dopt%3DAbstract In conclusion, D-MEM might be a suitable basal medium for the cultivation of MSCs for clinical applications. PMID: 18725693 [PubMed - in process] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1744247 Sat, 30 Aug 2008 15:06:19 +0100 1744247 http://www.medworm.com/index.php?rid=1744246&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18725694%26dopt%3DAbstract Authors: Visco AM, Campo N, Torrisi L, Cristani M, Trombetta D, Saija A Ultra high molecular weight polyethylene, both pure and electron beam irradiated, was exposed to air and hyaluronic acid. Physical and mechanical analyses were performed in order to check the polymeric change due to the treatment. Pure UHMWPE, in fact, was modified by the hyaluronic acid that plasticizes the polymer. The electron irradiation cross links the chains and prevents their sliding in acid presence. A low irradiation dose is preferable rather than an high one. Low doses induce low amount of free radicals, witch react with oxygen and hyaluronic acid inducing low polymer degrade maintaining the initial mechanical performance. High doses degrade significantly the polymer properties.100 kGy irradiation in air ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1744246 Sat, 30 Aug 2008 15:06:19 +0100 1744246 http://www.medworm.com/index.php?rid=1744245&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18725695%26dopt%3DAbstract Authors: Crowley J, Chalivendra VB An experimental study of Ultra-High Molecular Weight Polyethylene (UHMWPE) and hydroxyapatite (HAP) nanocomposites for the purpose of joint prosthesis is investigated under quasi-static and dynamic loading conditions. By employing compression mold process, five different volume fractions of hydroxyapatite nanoparticle reinforced nanocomposites are made. Quasi-static tension and compression tests are performed using ASTM test methods. Dynamic compression characterization is performed using split Hopkinson pressure bar technique. The nanocomposite performance as a function of volume fraction under a typical loading rate is investigated. The results of the fabricated nanocomposites are compared with currently employed UHMWPE in the joint implants. For co... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1744245 Sat, 30 Aug 2008 15:06:19 +0100 1744245 http://www.medworm.com/index.php?rid=1744244&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18725696%26dopt%3DAbstract Authors: Asano T, Tsuru K, Hayakawa S, Osaka A Among the clinical treatments of Familial Hyper cholesterolemia patients to reduce the concentration of low density lipoprotein (LDL), blood purification therapy is most suitable in which a blood-compatible adsorbent is employed. In the present study, alumina powders were prepared via a sol-gel route to develop a LDL-adsorbent Aluminum tri2-propoxide was hydrolyzed and subsequently calcined up to 1200 degrees C. Surface charge density and pore size distribution were measured, and the phases were identified. The alumina calcined above 400 degrees C had excellent blood compatibility in terms of endogenous clotting parameters, i.e., partial thromboplastin time: (PTT), prothrombin time: (PT), and the amount of fibrinogen: (Fib). The amount of ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1744244 Sat, 30 Aug 2008 15:06:19 +0100 1744244 http://www.medworm.com/index.php?rid=1590597&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18198402%26dopt%3DAbstract Authors: Shabalovskaya S, Anderegg J, Rondelli G, Vanderlinden W, De Feyter S Comparative fibrinogen adsorption and platelet morphology were evaluated on a wide array of well-characterized Nitinol surfaces (polished, chemically etched, boiled in water, electropolished in different electrolytes and heat treated). XPS, SEM, AFM, atomic adsorption spectroscopy and electrochemistry were employed to acquire information on surface chemistry, topography and Ni release. Obtained surfaces, of various topographies and crystallinity from mostly amorphous to nano-crystalline with Ni concentration from 1 to 8%, induced Ni release into biological medium in a subtoxic range (0-11 ng/ml/cm(2)). Fibrinogen adsorption to Nitinol surfaces ranged from that characteristic to pure Ni (130 ng/cm(2)) to pure ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590597 Mon, 07 Jul 2008 19:54:20 +0100 1590597 http://www.medworm.com/index.php?rid=1590596&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18198403%26dopt%3DAbstract Authors: Brochhausen C, Zehbe R, Gross U, Libera J, Schubert H, Nüsing RM, Klaus G, Kirkpatrick CJ Tissue engineering of articular cartilage remains an ongoing challenge. Since tissue regeneration recapitulates ontogenetic processes the growth plate can be regarded as an innovative model to target suitable signalling molecules and growth factors for the tissue engineering of cartilage. In the present study we analysed the expression of cyclooxygenases (COX) in a short-term chondrocyte culture in gelatin-based scaffolds and in articular cartilage of rats and compared it with that in the growth plate. Our results demonstrate the strong cellular expression of COX-1 but only a focal weak expression of COX-2 in the seeded scaffolds. Articular cartilage of rats expresses homogeneously C... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590596 Mon, 07 Jul 2008 19:54:20 +0100 1590596 http://www.medworm.com/index.php?rid=1590595&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18198404%26dopt%3DAbstract Authors: Fukui N, Sato T, Kuboki Y, Aoki H The purpose of this study was to develop a new biodegradable bone substitute materials consisting of synthesized nano-size hydroxyapatite (nano-HAp) and Type I biodegradable honeycomb collagen sponge (HCS) composites. Bone defects in rabbit mandibles were prepared by a drill, and the composites were implanted into the bone defects. The HCS only and the HCS/calcined hydroxyapatite (HAp) composite were used as comparative materials. The bone tissues reaction at the early stage within 3 weeks after implantaion was investigated histologically. Amounts of new bone formation were determined by NIH-image analysis software using the histological sections. The amounts of the new bone formation were largest in the nano HAp/HCS compared to the comparativ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590595 Mon, 07 Jul 2008 19:54:20 +0100 1590595 http://www.medworm.com/index.php?rid=1590594&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18198405%26dopt%3DAbstract Authors: John LC Sternal dehiscence is an important complication of cardiac surgery. A simple mathematical analysis is described for determining the relative strength (S) of different types of sternal closure. This is based upon resolving the tensions within the segments of a sternal wire to derive a dehiscence force. The strength of the closure (S) is the ratio of the volume of sternum which the dehiscence force acts upon to the dehiscence force itself. The derived S values varied from 4.3 for a closure using 6 transverse wires to 11.4 for a closure using the Modified Technique. The analysis suggested that closures which redistribute the transverse force to act obliquely upon a greater volume of sternum, such as the Modified Technique, are less likely to fail. PMID: 18198405 [PubM... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590594 Mon, 07 Jul 2008 19:54:20 +0100 1590594 http://www.medworm.com/index.php?rid=1590593&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18198406%26dopt%3DAbstract Authors: Joyce TJ The key joint of the forefoot during gait is the first metatarsophalangeal joint. It plays an important role in propelling the human form but can be subject to a number of diseases which can lead to its replacement with an artificial joint. Some of these designs of prosthesis employ a two-piece ball and socket arrangement and are available with a range of biomaterial couples including ceramic-on-ceramic, metal-on-metal and metal-on-polymer. Calculation of predicted lubrication regimes applicable to these implant designs was undertaken. Modelling the ball and socket implant as an equivalent ball-on-plane model and employing elastohydrodynamic theory allowed the minimum film thickness to be calculated and in turn the lambda ratio to indicate the lubrication regime. The ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590593 Mon, 07 Jul 2008 19:54:20 +0100 1590593 http://www.medworm.com/index.php?rid=1590592&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18408256%26dopt%3DAbstract This study aimed to show whether screw design altered the effects of stress relaxation on the mechanical performance of the pedicle screw during pullout. Twelve calf vertebrae were obtained: six vertebrae were instrumented with 7.5x40 mm conical pedicle screws and the other six with 5.0x40 mm cylindrical pedicle screws. The screws with two different designs were pulled out using either a standard pullout or a stress relaxation pullout protocol. Both bone-screw interfaces had lower stiffness in the stress relaxation pullout model than in the standard pullout model, but it was significant in only the cylindrical design group (P<0.05). However, the stress relaxation and standard pullout models did not yield any difference in peak loads in either screw type. Although stress relaxation at th... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590592 Mon, 07 Jul 2008 19:54:20 +0100 1590592 http://www.medworm.com/index.php?rid=1590591&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18408257%26dopt%3DAbstract Authors: Ubeyli ED The automated diagnostic systems employing diverse and composite features for electrocardiogram (ECG) signals were analyzed and their accuracies were determined. Because of the importance of making the right decision, classification procedures classifying the ECG signals with high accuracy were investigated. The classification accuracies of multilayer perceptron neural network (MLPNN), recurrent neural network (RNN), and mixture of experts (ME) trained on composite features and modified mixture of experts (MME) trained on diverse features were compared. The inputs of these automated diagnostic systems were composed of diverse or composite features (wavelet coefficients and power levels of the power spectral density estimates obtained by the eigenvector methods) and w... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590591 Mon, 07 Jul 2008 19:54:20 +0100 1590591 http://www.medworm.com/index.php?rid=1590590&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18408258%26dopt%3DAbstract Authors: Pylios T, Shepherd DE The metacarpophalangeal (MCP) joint is crucial for hand function, but the joints are frequently affected by arthritis, leading to pain and disability. Joint replacement implants are used to replace the diseased MCP joint. This paper presents an investigation of applying the soft layered concept in the design of a new MCP joint replacement implant. Analytical methods were used to investigate the minimum film thickness for a novel MCP joint with a soft layer. The effect of load, entraining velocity, radial clearance, radius of the metacarpal head, elastic modulus and thickness of the soft layer were investigated. The soft layered joints show an enhanced predicted film thickness and some evidence of fluid film lubrication that should help to reduce wear rate... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590590 Mon, 07 Jul 2008 19:54:20 +0100 1590590 http://www.medworm.com/index.php?rid=1590589&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18408259%26dopt%3DAbstract Authors: Lewis G, Son Y Heat-conduction isothermal microcalorimetry was used to measure the exothermic heat flow rate (Q) from the powder of a widely used commercially-available acrylic bone cement, Palacos R, when it interacted with ambient laboratory air, as a function of time, t, in the calorimeter, for up to 200 h. Four variants of the powder were used, these being unsterilized, sterilized using ethylene oxide gas, gamma-irradiated, in ambient air, with a minimum dosage of 2.5 Mrad, and gamma-irradiated, in ambient air, with a minimum dosage of 4.5 Mrad. In each case, the powder variant was tested after being stored on the shelf, under ambient conditions, for 2 days, 3 weeks and 9 months immediately following sterilization. Best-fit correlations between Q and t for each powder vari... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590589 Mon, 07 Jul 2008 19:54:20 +0100 1590589 http://www.medworm.com/index.php?rid=1590588&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18408260%26dopt%3DAbstract Authors: Palmieri A, Pezzetti F, Brunelli G, Martinelli M, Scapoli L, Arlotti M, Masiero E, Carinci F Porous polyethylene (PP or Medpor) is an alloplastic material worldwide used for craniofacial reconstruction. Although several clinical studies are available, there is a lack as regard the genetic effects. Because PP is always fixed on bone and the mechanism by which PP acts on osteoblasts is unknown, we therefore attempted to address this question by using microRNA microarray techniques to investigate the translation regulation in osteoblasts exposed to PP. The miRNA oligonucleotide microarray provides a novel method to carry out genome-wide microRNA profiling in human samples.By using miRNA microarrays containing 329 probe designed from Human miRNA sequence, we identified in osteobla... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590588 Mon, 07 Jul 2008 19:54:20 +0100 1590588 http://www.medworm.com/index.php?rid=1590587&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18408261%26dopt%3DAbstract In this study, breath ammonia concentration was continuously measured using a crystal oscillator QCM (quartz crystal microbalance) during the expiration of patients undergoing dialysis treatment. The results show that NH3 (ammonia) decreased gradually as the treatment proceeded. A strong correlation was observed between changes in the frequency of the QCM gas sensor and both the pre-dialysis BUN level (r=0.71, p<0.05) and the post-dialysis BUN level (r=0.90, p<0.05). NH3 was found to fall precipitously during dialysis. The differences were statistically significant. In addition, we found a statistically significant correlation between BUN and NH3 in expired gas. These results suggest that continuous measurement of NH3 is useful to assess the status of solute removal during hemodialys... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590587 Mon, 07 Jul 2008 19:54:20 +0100 1590587 http://www.medworm.com/index.php?rid=1590586&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334717%26dopt%3DAbstract Authors: Bieback K, Kern S, Kocaömer A, Ferlik K, Bugert P Mesenchymal stromal cells (MSC) are currently in focus because of their clinical potential in cell therapy and tissue engineering. As yet bone marrow represents the main source of MSC for both experimental and clinical studies. However, it is speculated that the clinical value might be diminished as both the number of MSC and their differentiation capacity decline with age. Alternatively, MSC have been successfully isolated from nearly every tissue attempted so far. Our work is focused on comparing MSC derived from human adult bone marrow, lipoaspirate as well as cord blood in terms of being alternatives containing high precursor frequencies and youngest adult cells. Applying identical culture conditions, major differences... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590586 Mon, 07 Jul 2008 19:54:20 +0100 1590586 http://www.medworm.com/index.php?rid=1590585&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334718%26dopt%3DAbstract Authors: Sensebé L Mesenchymal Stem Cells (MSCs) are multipotent adult stem cells having an immunosuppressive effect. These characteristics lead to an increasing use of MSC in graft process or for regenerative medicine. For the clinical uses of MSCs, standards are needed. The clinical grade production necessitates adhering to good manufacturing practices (GMP) to insure the delivery of a "cell drug" that is safe, reproducible and efficient. All parts of the process must be defined: the starting material (tissue origin, separation or enrichment procedures), cell density in culture, and medium (fetal calf serum (FCS) or human serum, cytokines with serum-free medium for target). But to reach the GMP goal, cells have to be cultured in as close to a closed system as possible. Analytica... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590585 Mon, 07 Jul 2008 19:54:20 +0100 1590585 http://www.medworm.com/index.php?rid=1590584&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334719%26dopt%3DAbstract Authors: De Bari C, Dell'accio F Cell therapies are unique in that the active component consists of living cells, which are difficult to define in their pharmacologic characteristics, and which produce variable and largely unknown amounts of bioactive molecules. Thus, the definition of the composition of a cellular product, mechanisms of action, pharmacokinetics, toxicity and efficacy assessment represent challenges never previously faced by traditional pharmacology. A pressing need for a routine use of cell therapies in the clinic is the development of quality controls for efficacy on the basis of clinically relevant potency assays, with prospective validation in human clinical trials. This review will focus on cell-based protocols for joint surface repair. In particular, we will pres... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590584 Mon, 07 Jul 2008 19:54:20 +0100 1590584 http://www.medworm.com/index.php?rid=1590583&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334720%26dopt%3DAbstract Authors: Decot V, Latger-Cannard V, Lecompte T, Clément L, Salmon A, Bordigoni P, Stoltz JF, Bensoussan D Chimerism analysis has become an important tool to manage patients in the peri-transplant period of allogenic stem cell transplantation. During this period, cells of donor and host origin can coexist and increasing proportion of cells of host origin is considered as a recurrence of the underlying disease. We currently performed chimerism analysis on separate peripheral blood cell subsets, lymphocytes and granulocytes. To improve our isolation method, a new automated device from Stem Cell Technology Roboseptrade mark was tested and compared to our manual separation technique. The results obtained on T cell purification showed an improvement of the purity (98.42% with Robosep vs... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590583 Mon, 07 Jul 2008 19:54:20 +0100 1590583 http://www.medworm.com/index.php?rid=1590582&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334721%26dopt%3DAbstract In conclusion, CTX may improve cardiac deformation and MR during exercise in patients with severe chronic heart failure when viable areas are targeted. PMID: 18334721 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590582 Mon, 07 Jul 2008 19:54:20 +0100 1590582 http://www.medworm.com/index.php?rid=1590581&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334722%26dopt%3DAbstract Authors: Chajra H, Rousseau CF, Cortial D, Ronzière MC, Herbage D, Mallein-Gerin F, Freyria AM Articular cartilage has a limited capacity for self-repair after trauma. Besides the conventional surgical techniques for repairing such defects, treatments involve implantation of autologous cells in suspension or within a variety of cell carrying scaffolds such as hyaluronic acid, alginate, agarose/alginate, fibrin or collagen. For the repair of full-thickness osteochondral defects, tissue engineers started to design single- or bi-phased scaffold constructs often containing hydroxyapatite-collagen composites, usually used as a bone substitute. The purpose of this study was to compare the behavior of bovine chondrocytes cultured in collagen-based scaffolds containing or not hydroxyapati... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590581 Mon, 07 Jul 2008 19:54:20 +0100 1590581 http://www.medworm.com/index.php?rid=1590580&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334723%26dopt%3DAbstract Authors: Wang Y, de Isla N, Huselstein C, Wang B, Netter P, Stoltz JF, Muller S To investigate whether the application of alginate culture and mechanical stimulation will improve the synthesis of cartilaginous matrix in dedifferentiated chondrocytes, rat chondrocytes underwent dedifferentiation upon serial monolayer culture up to passage 6, and then were encapsulated in 2% alginate gel and subject to static culture. After 28 days culture in static, the beads were exposed to 48 h of mechanical stimulation with continuous agitation. The sGAG content in alginate bead was measured by alcian blue staining. The expression of collagen protein was detected using immunofluorescence. After 28 days culture in alginate bead, the dedifferentiated chondrocytes remained round in shape and re-synthesi... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590580 Mon, 07 Jul 2008 19:54:20 +0100 1590580 http://www.medworm.com/index.php?rid=1590579&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334724%26dopt%3DAbstract In this study we compared the in vitro formation of cartilaginous grafts composed of collagen type I hydrogel with both ovine primary articular chondrocytes (AC) and bone marrow derived mesenchymal stem cells (MSC). During 4 weeks of culture, aggregate properties were quantitatively verified, cell viability and the expression of cartilage markers were assayed. Different microscopic techniques indicated a subdivision of MSC based scaffolds into a central construct region with uniformly distributed stem cells with low levels of apoptosis, and peripheral layers of proliferative cells, which undergo differentiation. Immunohistochemical staining and quantitative measurements of sulfated glycosaminoglycans (s-GAG) of MSC hydrogels showed a significant increase in matrix deposition, mainly in out... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590579 Mon, 07 Jul 2008 19:54:20 +0100 1590579 http://www.medworm.com/index.php?rid=1590578&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334725%26dopt%3DAbstract Authors: Kadi A, Fawzi-Grancher S, Lakisic G, Stoltz JF, Muller S Tissue engineering requires the response of the cells to different stimuli inducing the synthesis of the extracellular matrix (ECM). It was been shown that mechanical and biochemical stimuli acted on the synthesis of ECM, particularly type I and III collagens. Growth factors implied in transduction pathways are multiple, but the main is TGF-beta. Member of the transforming growth factor-beta (TGF-beta) family bind to type II and type I serine/threonine kinase receptors, which initiate intracellular signals through activation of SMADs proteins. Nevertheless, the effects of mechanical stress of this pathway remain unknown. The aim of this work was to study the pathway of TGF-beta via the SMADs proteins under mechanical (st... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590578 Mon, 07 Jul 2008 19:54:20 +0100 1590578 http://www.medworm.com/index.php?rid=1590577&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334726%26dopt%3DAbstract Authors: Builles N, Bechetoille N, Justin V, André V, Burillon C, Damour O To reconstruct artificial stroma close to corneal stroma, it is necessary to use keratocytes with high proliferative potential that maintain the keratocyte phenotype as characterised by CD34. To select such cells, we tested the proliferative potential and characterised the keratocytes isolated from 4 different areas of the human cornea: superior perilimbal, inferior perilimbal, superior central and inferior central. Keratocytes isolated from these different areas had significantly different growth rates (p<0.05), as measured by population doublings: superior perilimbal (42.59+/-11.78) > inferior perilimbal (38.23+/-12.67) > superior central (35.69+/-8.07) > inferior central (25.35+/-7.63). Their... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590577 Mon, 07 Jul 2008 19:54:20 +0100 1590577 http://www.medworm.com/index.php?rid=1590576&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334727%26dopt%3DAbstract Authors: de Isla NG, Mainard D, Muller S, Stoltz JF Osteoarthritis (OA) is a progressive joint disease which represents a combination of several disorders leading to cartilage degradation. The production of nitric oxide (NO) by iNOS, which is stimulated by proinflammatory mediators, is involved in cartilage catabolism and should therefore be considered a potential target for therapeutic interventions. Diacerein is a slow-acting drug that may slow down the breakdown of cartilage and relieve pain and swelling. Diacerhein, in contrast to an NSAID, is a potent inhibitor of IL-1beta induced NO production by chondrocytes and cartilage. This effect appeared to result from iNOS transcriptional and/or post-transcriptional events, maybe by the inhibition of the NF-kappaB transcription factor. Th... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590576 Mon, 07 Jul 2008 19:54:20 +0100 1590576 http://www.medworm.com/index.php?rid=1590575&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18334728%26dopt%3DAbstract Authors: Féréol S, Fodil R, Laurent VM, Planus E, Louis B, Pelle G, Isabey D The sensitivity of alveolar macrophages to substrate properties has been described in a recent paper (Féréol et al., Cell Motil. Cytoskel. 63 (2006), 321-340). It is presently re-analyzed in terms of F-actin structure (assessed from 3D-reconstructions in fixed cells) and mechanical properties (assessed by Magnetic Twisting Cytometry experiments in living cells) of cortical and deep cytoskeleton structures for rigid plastic (Young Modulus: 3 MPa) or glass (70 MPa) substrates and a soft (approximately 0.1 kPa) confluent monolayer of alveolar epithelial cells. The cortical cytoskeleton component (lowest F-actin density) is represented by the rapid and softer viscoelastic compartment while the ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590575 Mon, 07 Jul 2008 19:54:20 +0100 1590575 http://www.medworm.com/index.php?rid=1590574&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18533324%26dopt%3DAbstract Authors: PMID: 18533324 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590574 Mon, 07 Jul 2008 19:54:20 +0100 1590574 http://www.medworm.com/index.php?rid=1590638&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17264382%26dopt%3DAbstract In this study, CPC scaffolds with a single central channel were fabricated indirectly using a stereolithography rapid prototyping (RP) technology. The diameters of the central channels ranged from 402 microm to 1988 microm. These scaffolds were seeded with rabbit marrow stem cells (MSCs) labeling DiI and cultured for 5 days. Cell observation on the wall of the central channels was performed. The data of the experimental point revealed that cell coverage was from approximately 18% (1988 microm) to approximately 35% (592 microm). There was a significant increase from day 1 to day 5 in cell coverage in the same channel. The cell area coverage increased lineally with the central channel diameter when the channel diameter was less than approximately 789 microm. Afterwards (from 789 to 1988 micr... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590638 Mon, 01 Jan 2007 05:00:00 +0100 1590638 http://www.medworm.com/index.php?rid=1590637&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17264383%26dopt%3DAbstract Authors: Gagnieu CH, Forest PO Oxidized glycogen is used as a collagen crosslinker to prepare materials with defined crosslinking rates. Thus, films are prepared from native or denatured porcine type I atelocollagen crosslinked with three crosslinking levels defined by the ratios between the aldehyde groups of the glycogen and the amino groups of the collagen. The remaining free aldehyde groups and the imine bonds formed in the reaction are subsequently reduced or not. All the materials are subjected to in vivo biocompatibility and biodegradability evaluations by subcutaneous implantation in mice, while immunogenicity is evaluated by rabbit immunizations. As a result, cellular reactions on the implantation site are more important with nonreduced materials, and biodegradability is corre... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590637 Mon, 01 Jan 2007 05:00:00 +0100 1590637 http://www.medworm.com/index.php?rid=1590636&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17264384%26dopt%3DAbstract Authors: Sarathi Kopparti P, Lewis G A finite element analysis of the stresses in a three-dimensional model of the proximal human tibia containing the tibial components (insert, tray, and stem) of a cemented total knee joint replacement was conducted. 12 cases of this model, covering three variables (composition of applied loading, interface conditions, and assignment of elastic properties to the cortical and cancellous bones) were analyzed. It was found that the stresses in various parts of the model were markedly lower when (a) the applied load comprised a compressive joint reaction force (F), of 2 kN, only (quadriceps inactive) versus F+ a patellar ligament force of 1.46 kN (P), representing the situation when the quadriceps are active; and (b) the interfaces were considered fully b... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590636 Mon, 01 Jan 2007 05:00:00 +0100 1590636 http://www.medworm.com/index.php?rid=1590635&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17264385%26dopt%3DAbstract Authors: Hesaraki S, Sharifi D Calcium phosphate cements (CPCs) are biocompatible and osteoconductive materials used in dental, craniofacial and orthopaedic applications. One of the most important advantages of these materials is their replacement with bone followed by resorption. Already several attempts have been made to improve the resorption behaviour of calcium phosphate cements by increasing the porosity of the material. In this investigation a mixture of NaHCO(3) and citric acid monohydrate was added to the apatite cement component as an effervescent additive for producing interconnected macropores into the cement matrix. Mercury intrusion porosimetry was employed to determine pore volume and pore size distribution in the calcium phosphate cement (CPC) samples. Results showed th... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590635 Mon, 01 Jan 2007 05:00:00 +0100 1590635 http://www.medworm.com/index.php?rid=1590634&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17264386%26dopt%3DAbstract Authors: Luis AL, Rodrigues JM, Lobato JV, Lopes MA, Amado S, Veloso AP, Armada-da-Silva PA, Raimondo S, Geuna S, Ferreira AJ, Varejão AS, Santos JD, Maurício AC The purpose of this study was to test in vivo two different nerve guides, one of PLGA made of a novel proportion (90:10) of the two polymers, Poly(L-lactide):Poly(glycolide), with (DL-lactide-epsilon-caprolactone) copolyester (Neurolac) tube, in promoting nerve regeneration across a 10 mm-gap of the rat sciatic nerve. Finally, end-to-end coaptation was performed. Motor and sensory functional recovery was assessed throughout the healing period of 20 weeks and the repaired nerves were processed for morphological analysis. Both motor and sensory functions improved significantly in all experimental nerve repair groups, a... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590634 Mon, 01 Jan 2007 05:00:00 +0100 1590634 http://www.medworm.com/index.php?rid=1590633&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17264387%26dopt%3DAbstract In this study, the cp-Ti surfaces were prepared to enable machined surfaces (TM) to be compared to the machined, sandblasted, laser irradiated and dual-acid etched surfaces (TA). The surface elements and roughness were characterized. The biocompatibility was evaluated by cell and organ culture in vitro. The removal torque was measured in rabbit implantation. Surface characterization revealed that TA surface was more oxidized than TM surface. The TA surface had micrometric, beehive-like coarse concaves. The average roughness (2.28 mum) was larger than that typical of acid-etched surfaces. Extracts of both materials were not cytotoxic to bone cells. The morphology of cells attached on the TA surface was superior to that on the TM surface. TA promoted cell migration and repaired damaged bones... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590633 Mon, 01 Jan 2007 05:00:00 +0100 1590633 http://www.medworm.com/index.php?rid=1590632&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17377215%26dopt%3DAbstract Authors: Mohd Janurudin J, Ozeki K, Aoki H, Fukui Y Hydroxyapatite (HA)/Hydrogen Peroxide (H(2)O(2)) solutions was prepared for a tooth whitening application. HA powder was immersed in the 100, 1000 and 10,000 ppm of the H(2)O(2) solutions. The HA/H(2)O(2), HA and H(2)O(2) solutions were applied to HA discs and extracted teeth for weight and whiteness change evaluation. After 24 hours, each HA disc was measured for the weight changes and each tooth was measured for the whitening changes (L(*)) using a Spectro Color Meter. The topology of the teeth surfaces were also observed using Scanning Electron Microscope (SEM). The weight of the HA disc applied with H(2)O(2) at all concentrations was lower than that applied with HA and HA/H(2)O(2) solutions. The whiteness of the teeth treated with... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590632 Mon, 01 Jan 2007 05:00:00 +0100 1590632 http://www.medworm.com/index.php?rid=1590631&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17377216%26dopt%3DAbstract Authors: Maeda E, Noguchi H, Tohyama H, Yasuda K, Hayashi K The central one-third portion of the patellar tendon (PT) is commonly used for the reconstruction of the anterior cruciate ligament. For better understanding of the healing mechanisms of the PT, tensile properties of collagen fascicles harvested from the healing PT were studied. A rectangular defect was made at the central third portion in each right PT in the skeletally mature rabbit. At 6 and 12 weeks, tensile tests were performed on fascicles from the tissue regenerated in the defect and the non-resected, residual tissue. The elastic modulus and tensile strength of fascicles from the regenerated tissue gradually increased in a fashion similar to the bulk regenerated tissue. The properties of fascicles from the residual tiss... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590631 Mon, 01 Jan 2007 05:00:00 +0100 1590631 http://www.medworm.com/index.php?rid=1590630&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17377217%26dopt%3DAbstract Authors: Kobayashi K, Kakinoki T, Sakamoto M, Tanabe Y The effects of strain rate and low-gamma irradiation on the compressive properties of ultra-high molecular-weight polyethylene (UHMWPE) were investigated. Gamma irradiation was performed at 30 kGy in nitrogen or at 29 kGy in air without post-irradiation treatment. Quasi-static and impact compressive tests using the split-Hopkinson pressure-bar technique were performed to measure stress-strain relations up to a true strain of 8% at strain rates of between 0.0004 and 260 s(-1). For both unirradiated and gamma-irradiated UHMWPE specimens, an increase in the strain rate significantly increased the Young's modulus and the 0.5% yield stress. Gamma irradiation in air significantly increased the Young's modulus, as determined by quasi-stat... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590630 Mon, 01 Jan 2007 05:00:00 +0100 1590630 http://www.medworm.com/index.php?rid=1590629&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17377218%26dopt%3DAbstract The objective of this study was to test the hypothesis that there is one model for the interface between the passive layer on the surface of an orthopaedic alloy and an electrolyte that has clinical relevance. To this end, electrochemical impedance spectroscopy measurements were made on mechanically-polished test specimens fabricated from three orthopaedic alloys [two Ti-based ones (Ti-6Al-4V and Ti-13Nb-13Zr) and a very-low-nickel austenitic stainless steel, Biodur 108] while they were immersed in phosphate buffered saline (PBS) solution, at 37+/-1 degrees C, over a period of 25 d. Through a statistical comparison between the experimental results (Nyquist and Bode plots) and those computed using seven different equivalent electrical circuits (each representing a different model of the afo... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590629 Mon, 01 Jan 2007 05:00:00 +0100 1590629 http://www.medworm.com/index.php?rid=1590628&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17377219%26dopt%3DAbstract This study examines the effect of poison (2-deoxy-D-glucose) and strain on fibroblast viability and stress-relaxation in skin samples from the backs of 20 same-age male rats. Skin samples were either soaked in Kreb's solution or in poison and was then either strained or left unstrained, for a total of four different treatment groups. All samples were fixed and processed for apoptosis assay and light microscopy. The viability study showed that strained tissues soaked in Kreb's solution had significantly more apoptotic cells compared to unstrained tissues soaked in the same solution. For tissues soaked and strained in poison the increase in apoptotic cells was negligible. Samples strained in Kreb's solution were found to have greater stress relaxation compared to samples strained in Kreb's s... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590628 Mon, 01 Jan 2007 05:00:00 +0100 1590628 http://www.medworm.com/index.php?rid=1590627&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17377220%26dopt%3DAbstract Authors: Farmanzad F, Najarian S, Eslamee MR, Seddighi AS To obtain an appropriate model for the simulation of the biomechanical behavior of brain tissue and the deformation of ventricles, in particular, we have developed a novel computerized plain strain finite element model. For optimum results, a multiple loading solutions approach using various tissue parameters for the simulation of epidural hematoma have been tested. For this purpose, CT-Scan of a patient with traumatic epidural hematoma has been modeled. By changing the tissue parameters (E and nu) and increasing intraventricular pressure gradient, the displacement of similar points in the modeled ventricle was compared with the true values obtained from patient's CT-Scan taken 3 months later after the resolution of hematoma. Th... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590627 Mon, 01 Jan 2007 05:00:00 +0100 1590627 http://www.medworm.com/index.php?rid=1590626&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17377221%26dopt%3DAbstract In this study we analyzed the effects of the alloy, in comparison with those of other dental metals, on the adhesion, spreading, and proliferation of human gingival fibroblasts (Gin-1 cells) in vitro. The Gin-1 cells adhered and spread well on the Ti-Ta as well as on commercially pure titanium (Ti) and commercial Ti-6Al-7Nb alloy (Ti-Al), forming long processes showing a typical fibroblastic morphology that was close to that on glass. The proliferation of Gin-1 cells was significantly suppressed on Au-Pd-Ag alloy (Au-Pd) and commercially pure copper (Cu); however, the cells proliferated as well on Ti-Ta as they did on Ti, Ti-Al, and glass. Though most of the Gin-1 cells on Cu and about half of them on Au-Pd died after 1 day and 5 days of culture, respectively, the cells on Ti-Ta, Ti, Ti-Al... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590626 Mon, 01 Jan 2007 05:00:00 +0100 1590626 http://www.medworm.com/index.php?rid=1590625&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502690%26dopt%3DAbstract Authors: Chueh SK, Tachibana Y, Uyama H, Kobayashi S, Tomita N Poly(N-substituted alpha/beta-asparagine) was evaluated as a thermoresponsible and an injectable scaffold for cartilage regeneration. Solutions of this polymer are liquid state below 25 degrees C and nonfluid hydrogel above 35 degrees C, allowing an aqueous solution containing cells at room temperature to form a hydrogel with encapsulated cells at physiological body temperature. Chondrocytes were isolated from joint of 4-week-old Japanese white rabbits, dispersed within the thermoresponsive polymer solution and maintained for up to 72 hours in vitro. The polymer solutions demonstrated concentration-dependent inhibitory effect on chondrocytes multiplication. After the three-day cultivation, the survival rate of the chondrocy... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590625 Mon, 01 Jan 2007 05:00:00 +0100 1590625 http://www.medworm.com/index.php?rid=1590624&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502691%26dopt%3DAbstract Authors: Leong KF, Wiria FE, Chua CK, Li SH Selective Laser Sintering (SLS), an established Rapid Prototyping (RP) process, is investigated for building controlled drug delivery devices (DDD). The drug and its matrix in a powder form were first mixed mechanically before being sintered on the SLS. Each cylindrical DDD is designed with a number of concentric rings separated from each other by a characteristic 'wall' created by the laser of the SLS. These rings act as diffusion obstacles to control the rate of release. Poly-epsilon-caprolactone (PCL) was used as the matrix and Methylene Blue (MB) as the drug model. Samples were built, characterized and tested for homogeneity using Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), and Fourier Transform Infrared Spe... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590624 Mon, 01 Jan 2007 05:00:00 +0100 1590624 http://www.medworm.com/index.php?rid=1590623&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502692%26dopt%3DAbstract Authors: Sethumadhavan R, Taguchi T, Tanaka J, Tateishi T A novel tissue adhesive consisting of human serum albumin (HSA) and tartaric acid derivative (TAD) was developed by us. Four different concentrations of TAD namely, 0.05 mmol, 0.1 mmol, 0.2 mmol and 0.3 mmol were mixed with 40%, 42% and 44% HSA individually and were made in the form of disks. J774A.1 mouse macrophage cells were seeded on top of these disks. The disks were pre-treated with sterile water and Eagle's medium before every seeding. All the seeding was incubated from 1 day to 3 days before making any investigations on it. SEM images were recorded and it was observed that these cells adhered to these materials very well. Nitric oxide activity was studied using nitrate/nitrite colorimetric assay method. It was observed t... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590623 Mon, 01 Jan 2007 05:00:00 +0100 1590623 http://www.medworm.com/index.php?rid=1590622&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502693%26dopt%3DAbstract Authors: Wenzel SA, Shepherd DE Total disc arthroplasty is gaining in popularity as an alternative to spinal fusion. A total disc consists of articulating bearing surfaces with one made from a metal and the other made from either a metal or a polymer. The aim of this study was to determine the contact stresses in lumbar total disc arthroplasty devices. The total disc was modelled as a ball and socket joint and Hertzian contact theory was used to determine the maximum contact stresses. The effect of material combination and implant size on contact stress was investigated. For a typical disc arthroplasty with a ball radius of 14 mm, the contact stresses for metal against polymer and metal against metal material combinations were 3 to 6 MPa and 63-130 MPa, respectively, and were below the... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590622 Mon, 01 Jan 2007 05:00:00 +0100 1590622 http://www.medworm.com/index.php?rid=1590621&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502694%26dopt%3DAbstract Authors: Ozeki K, Kobayashi S, Hirakuri KK, Aoki H, Fukui Y Diamond-like carbon (DLC) films were deposited on ultra-high molecular weight polyethylene (UHMWPE) and polymethylmethacrylate (PMMA) with oxygen plasma pre-treatment using a radiofrequency plasma chemical vapour deposition method. A ball-on-disc test was carried out to evaluate the wear properties of the DLC-coated UHMWPE and PMMA. After testing, the surface of the polymers was observed using an atomic force microscope and an optical microscope. The adhesive strength of the DLC films deposited on the polymers was measured using a scratch test. After the ball-on-disc test, many cracks were observed in the films on the surface of both the DLC-coated UHMWPE and PMMA without the oxygen plasma pre-treatment, whereas the DLC-coated... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590621 Mon, 01 Jan 2007 05:00:00 +0100 1590621 http://www.medworm.com/index.php?rid=1590620&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502695%26dopt%3DAbstract Authors: Ubeyli ED This paper intends to an integrated view of the spectral analysis techniques in the detection of arterial disorders. The paper includes illustrative information about feature extraction from signals recorded from arteries. Short-time Fourier transform (STFT) and wavelet transform (WT) were used for spectral analysis of ophthalmic arterial (OA) Doppler signals. Using these spectral analysis methods, the variations in the shape of the Doppler spectra as a function of time were presented in the form of sonograms in order to obtain medical information. These sonograms were then used to compare the applied methods in terms of their frequency resolution and the effects in determination of OA stenosis. The author suggest that the content of the paper will assist to the peop... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590620 Mon, 01 Jan 2007 05:00:00 +0100 1590620 http://www.medworm.com/index.php?rid=1590619&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17502696%26dopt%3DAbstract Authors: Sakai Y, Matsuyama Y, Takahashi K, Sato T, Hattori T, Nakashima S, Ishiguro N For peripheral nerve regeneration, three-dimensional distribution and growth of cells within the porous scaffold are of clinical significance. We demonstrate that cultured rat Schwann cells and neurospheres grow in vitro on new artificial nerve conduits made with photocrosslinked hyaluronic acid (HA). HA tubular conduits have an inner diameter of 1.2 mm with porous nano-structure of 50 microm. After 3 weeks of cultivation, HA conduits remained circular with a round lumen, and construct of cell-conduits maintained the size and shape of the original architecture of the tube. HA itself has the function to facilitate a pathway for cellular and axonal ingrowth during peripheral nerve regeneration. These f... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590619 Mon, 01 Jan 2007 05:00:00 +0100 1590619 http://www.medworm.com/index.php?rid=1590618&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17611295%26dopt%3DAbstract Authors: Forest P, Morfin F, Bergeron E, Dore J, Bensa S, Wittmann C, Picot S, Renaud FN, Freney J, Gagnieu C In the last few years, regulations for biomolecule production, and especially for extraction and purification of animal molecules such as collagen, have been reinforced to ensure the sanitary safety of the materials. To be authorized to market biomaterials based on collagen, manufacturers now have to prove that at least one step of their process is described in guidelines to inactivate prion, viruses, and bacteria. The present study focuses on the inactivation step performed during the extraction and purification of porcine type I atelocollagen. We chose to determine the reduction factor of a 1 M NaOH step on porcine parvovirus and four bacterial strains inactivation. During th... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590618 Mon, 01 Jan 2007 05:00:00 +0100 1590618 http://www.medworm.com/index.php?rid=1590617&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17611296%26dopt%3DAbstract Authors: Sethumadhavan R, Taguchi T, Tanaka J, Tateishi T We developed a novel tissue adhesive consisting of human serum albumin (HSA) and tartaric acid derivative (TAD). Four different concentrations of TAD namely, 0.05 mM, 0.1 mM, 0.2 mM and 0.3 mM were mixed with 40%, 42% and 44% HSA individually and were made in the form of disks. J774A.1 mouse macrophage cells were seeded on top of these disks. The disks were pre-treated with sterile water and Eagle's medium before every seeding. All the seeding was incubated from 1 day to 3 days before making any investigations on it. SEM images were recorded and it was observed that these cells adhered to these materials very well. Mouse IL-6 cytokine expressions were studied using ELISA. It was seen from the cytokine expression results that the... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590617 Mon, 01 Jan 2007 05:00:00 +0100 1590617 http://www.medworm.com/index.php?rid=1590616&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17611297%26dopt%3DAbstract CONCLUSIONS: SUV in DHC images could be obtained in our method; the value in DHC images was lower than that in PET image; and many factors, such as system performance, lesion's size, and reconstruction algorithm could influence the SUV accuracy in both DHC and PET. PMID: 17611297 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590616 Mon, 01 Jan 2007 05:00:00 +0100 1590616 http://www.medworm.com/index.php?rid=1590615&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17611298%26dopt%3DAbstract In this study and for the first time, three different models of ST-LVAD are analyzed numerically. In the first model, the motion of the elastic membrane wall is simplified, while in the second model, the motion is assumed to be wavy. The pressure boundary conditions are added to the second model to allocate for the effect of pressure on the flow pattern, and hence, form the third model. The simulation results of the analyzed models show that in this particular type of LVAD, the viscous term of the applied stress from the fluid on the moving wall is negligible, compared with the pressure term. Additionally, it can be concluded that the motion pattern of the moving wall does not affect the blood flow pattern in a great deal. Furthermore, the inclusion of the fluid pressure in the boundary co... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590615 Mon, 01 Jan 2007 05:00:00 +0100 1590615 http://www.medworm.com/index.php?rid=1590614&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17611299%26dopt%3DAbstract Authors: Najarian S, Dargahi J, Farmanzad F In this work, a finite element formulation for the analysis of the elastodynamic behavior of the human aorta is presented. In this formulation, a one-dimensional approach was adopted and a comprehensive computer program was written and employed in the mathematical analysis. All the necessary material and geometrical parameters were appropriately incorporated in the simulation. A comparison was made between the simplified elasticity theory and the one proposed in this study using the poroelasticity theory. The effects of certain parameters including the fluid density and the material permeability of the matrix on the behavior of the aortic tissue were investigated. According to these findings, the higher the density of the liquid in the tissue... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590614 Mon, 01 Jan 2007 05:00:00 +0100 1590614 http://www.medworm.com/index.php?rid=1590613&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17611300%26dopt%3DAbstract Authors: Wang Y, Wei HW, Yu TC, Cheng CK Few studies have stressed on the sensitivity of stress distribution in different mechanical properties of the articular cartilage and subchondral bone. The purpose of this study was to establish parametric variations of mechanical factors individually and examine how these biomechanical effects influenced the cartilage and subchondral bone plate stress fields in the hip joint. A finite element model including acetabulum and proximal femur was established to study the stress change associated with the thinning of cartilage, the increasing of subchondral bone modulus and the thickening of subchondral bone plate individually. The stress distributions in bone/cartilage interface were evaluated. Sensitivity of the stress magnitudes to the parametric ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590613 Mon, 01 Jan 2007 05:00:00 +0100 1590613 http://www.medworm.com/index.php?rid=1590612&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17611301%26dopt%3DAbstract Authors: Paruchuru SP, Wang X, Agrawal CM The current methods to measure bone fracture toughness were initially developed for engineering materials and use specimen configurations that demand large samples. However, in many cases it is hard if not impossible to obtain such specimens from limited bone stock. Therefore, a new compact sandwich (CS) test method was formulated to measure the critical strain energy release rate (a measure of fracture toughness) of bone requiring only small samples. This technique may be used to assess bone fracture toughness and subsequently the bone quality. PMID: 17611301 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590612 Mon, 01 Jan 2007 05:00:00 +0100 1590612 http://www.medworm.com/index.php?rid=1590611&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17851168%26dopt%3DAbstract Authors: Viano AM, Spence KE, Shanks MA, Scott MA, Redfearn RD, Carlson CW, Holm TA, Ray AK Ultra-High-Molecular-Weight-Polyethylene (UHMWPE) is the material of choice for one of the articulating surfaces in many total joint replacements, notably hip and knee prostheses. The various methods used by the orthopaedic biomaterials industry to sterilize and anneal UHMWPE components, and the resulting oxidation and crosslinking, affect the mechanical wear resistance properties in ways still unknown at the microscopic and molecular levels. Transmission electron microscopy and chemical pyrolysis were used to quantify crosslinking induced by gamma irradiation and annealing in air. Changes in lamellar stacking and the amount of crosslinking suggest two types of crosslinking: relatively unstable ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590611 Mon, 01 Jan 2007 05:00:00 +0100 1590611 http://www.medworm.com/index.php?rid=1590610&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17851169%26dopt%3DAbstract This study investigated whether chondrocytes cultured through various passages maintain their potential to reexpress a chondrogenic phenotype in three-dimensional scaffolds and form cartilage tissue in vitro and in vivo. The growth rate, viability, synthesis of collagen type I and II, and apoptotic activity of chondrocytes with passage number of 1, 2 and 5 were compared during in vitro culture. As the passage number increased, the cell growth rate and viability decreased and apoptotic cell increased. Passage 2 chondrocytes exhibited a high expression of collagen type II and a low expression of collagen type I. In contrast, passage 5 chondrocytes exhibited a low expression of collagen type II and a high expression of collagen type I, indicating chondrocyte dedifferentiation. To examine the ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590610 Mon, 01 Jan 2007 05:00:00 +0100 1590610 http://www.medworm.com/index.php?rid=1590609&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17851170%26dopt%3DAbstract Authors: Santoni BG, Pluhar GE, Motta T, Wheeler DL Synthetic grafting materials, such as calcium phosphates (hydroxyapatite, HA; tricalcium phosphate, TCP), polymers, or composites thereof, can be used as osteoconductive scaffolds and delivery vehicles for osteoinductive growth factors. Carrier materials must be engineered to deliver these factors in a controlled fashion at a rate and dose consistent with the biological need and responsiveness of the system to optimize bone formation and ingrowth. They should also simultaneously provide mechanical support and slowly resorb as new bone is formed. This investigation assessed the elution characteristics of BMP-7 (OP-1) from hollow calcium phosphate spheres of varying chemical composition (HA/beta-TCP) and porosity (dense/porous). The pha... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590609 Mon, 01 Jan 2007 05:00:00 +0100 1590609 http://www.medworm.com/index.php?rid=1590608&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17851171%26dopt%3DAbstract Authors: Wan T, Aoki H, Hikawa J, Lee JH A sputtering technique is an effective method for surface modification of materials, but there are many complex process parameters to influence on the physical and chemical properties of the sputtered coating films. In this paper the process parameters were investigated when the hydroxyapatite (HA) was coated onto various substrates including titanium (Ti), alumina ceramic (Al(2)O(3)) and stainless steel (SUS) plates under various sputtering conditions, target type, Ar gas pressure, and discharge power. The deposition rate of HA was much higher in a solid plate target than in a powder lump target owing to the difference of apparent density 75%, 18%, respectively. Ar gas pressure little influenced on the deposition rate. The HA coating thickness ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590608 Mon, 01 Jan 2007 05:00:00 +0100 1590608 http://www.medworm.com/index.php?rid=1590607&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17851172%26dopt%3DAbstract CONCLUSION: Fibre-adhesive composite is a promising technique for fixating cervical vertebral fractures. PMID: 17851172 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590607 Mon, 01 Jan 2007 05:00:00 +0100 1590607 http://www.medworm.com/index.php?rid=1590606&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17851173%26dopt%3DAbstract This study shows that the maturation mode of the samples directly affects the mechanical properties of the obtained composites. The sterilization of the cement with chitosan has been studied. PMID: 17851173 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590606 Mon, 01 Jan 2007 05:00:00 +0100 1590606 http://www.medworm.com/index.php?rid=1590605&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17851174%26dopt%3DAbstract Authors: Li G, Huang J, Li Y, Zhang R, Deng B, Zhang J, Aoki H Influence of a discrete nano-hydroxyapatite crystal (nano-HAp) on lymphatic leukemia P388 cell behavior was investigated by an in vitro technique using an MTT test and FITC analysis. The discrete nano-HAp was prepared by decanting upside clear layer of the nano-HAp suspension which was synthesized by a wet method using calcium hydroxide suspension and phosphoric acid solutions. The nano-HAp was identified as hydroxyapatite by the X-ray powder diffraction pattern and an infrared spectroscopy. The nano-HAp with concentrations of 8-40 microg/ml was dispersed into PRMI 1640 media with leukemia cells derived from BALB/C mice. The survival ratio of the cells decreased with the amounts of the nano-HAp increasing. Apoptosis rates o... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590605 Mon, 01 Jan 2007 05:00:00 +0100 1590605 http://www.medworm.com/index.php?rid=1590604&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D17851175%26dopt%3DAbstract Authors: Meek MF PMID: 17851175 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590604 Mon, 01 Jan 2007 05:00:00 +0100 1590604 http://www.medworm.com/index.php?rid=1590603&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18032815%26dopt%3DAbstract Authors: Li X, Huang J, Ahmad Z, Edirisinghe M Electrohydrodynamic spray deposition of a hydroxyapatite (HA) suspension consisting of nano-particles has been used to create a hydroxyapatite coating comprising of nanostructured surface topography. Preliminary coating experiments were carried out on an Al substrate and 30 s was found to be the most appropriate coating time. HA coating on titanium for this duration was found to be well-bonded to the substrate after heat-treatment. A thickness of 2 mum was achieved in 30 s and formation of a bone-like apatite on the surface was detected after incubation of the heat-treated coated Ti in simulated body fluid. Therefore, we have uncovered a new procedure by which nano-biomaterials can be deposited on real orthopedic substrates to prepare bioa... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590603 Mon, 01 Jan 2007 05:00:00 +0100 1590603 http://www.medworm.com/index.php?rid=1590602&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18032816%26dopt%3DAbstract In conclusion degradation and replacement of di-/tri-calciumphosphate seems to be less accelerated in patients than prior animal studies indicated. Nevertheless, it shows excellent biocompatibility, good osteoconductive characteristics and may represents a useful alternative to autogenous graft. PMID: 18032816 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590602 Mon, 01 Jan 2007 05:00:00 +0100 1590602 http://www.medworm.com/index.php?rid=1590601&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18032817%26dopt%3DAbstract Authors: Mueller-Rath R, Gavénis K, Gravius S, Andereya S, Mumme T, Schneider U The nude mouse model is an established method to cultivate and investigate tissue engineered cartilage analogues under in vivo conditions. One limitation of this common approach is the lack of appropriate surrounding articular tissues. Thus the bonding capacity of cartilage repair tissue cannot be evaluated. Widely applied surgical techniques in cartilage repair such as conventional and three-dimensional autologous chondrocyte implantation (ACI) based on a collagen gel matrix cannot be included into nude mouse studies, since their application require a contained defect. The aim of this study is to apply an organ culture defect model for the in vivo cultivation of different cell-matrix-constructs.Cartil... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590601 Mon, 01 Jan 2007 05:00:00 +0100 1590601 http://www.medworm.com/index.php?rid=1590600&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18032818%26dopt%3DAbstract Authors: Kuraishi K, Ishida F, Kubo Y, Niwa S, Mizuno M, Umeda Y, Waga S, Taki W In an attempt to study bone remodeling by noninvasive methods, spinal bone radiodensity was assessed in five patients treated with anterior cervical decompression and fusion (ACDF) using cylindrical titanium cages. Plain radiographs were used to study specific areas of vertebral bone interposed in two-level cages with the two cephalad vertebrae for controls. Measurements were made immediately after surgery and 1, 3, 6, 12 and 18 months postoperatively. The data were analyzed quantitatively with a contrast-comparing method (CCM) using "Scion image". There were two cyclical changes in vertebral remodeling. First, in all patients there were gradual increases in bone density at the ventral part compared to the... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590600 Mon, 01 Jan 2007 05:00:00 +0100 1590600 http://www.medworm.com/index.php?rid=1590599&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18032819%26dopt%3DAbstract Authors: Guerin SR, MacNiochaill R, O'Reilly P, O'Byrne J, Kelly DJ Hydrogen peroxide has been used for decades as an effervescent haemostatic agent in arthroplasty. Recently it has been shown to adversely affect the material properties of PMMA. We aim to assess whether any such deleterious effects are demonstrated in an experimental model which mimics the clinical use of hydrogen peroxide. Matched pairs of cancellous bone samples were treated with a swab soaked in either saline or a 3% solution of hydrogen peroxide, prior to manufacture of cement-bone constructs using Palacos or Simplex cement. Thirty pairs were then compared by subjecting them to a torsional shear force until failure and a further thirty pairs were tested to failure in tension. There was no significant difference bet... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590599 Mon, 01 Jan 2007 05:00:00 +0100 1590599 http://www.medworm.com/index.php?rid=1590598&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D18032820%26dopt%3DAbstract Conclusions: nHAP films favored cell attachment and proliferation but not osteogenic differentiation of BMSCs compared with cHAP films. PMID: 18032820 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590598 Mon, 01 Jan 2007 05:00:00 +0100 1590598 http://www.medworm.com/index.php?rid=1590673&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823105%26dopt%3DAbstract Authors: Shalaw FG, Slimani S, Kolopp-Sarda MN, Marchand M, Faure G, Stoltz JF, Muller S It is well known today that mechanical forces are one of the important factors that induce a variety of cellular responses including morphological changes, protein synthesis, and gene expression and which are involve in tissue remodelling. We studied the effect of uniaxial cyclic stretching on the proliferation, collagens, and tenascin C mRNA expression of fibroblasts under different concentrations of foetal bovine serum. Proliferation was studied by cell cycle analysis, mRNA expression of collagen and tenascin C was studied by RT-PCR. Human fibroblasts were grown in silicon sheet coated with 1% gelatin. Cyclic stretching (5% elongation) was applied at 0.5 Hz (30 cycle/min), for 24 h with two conce... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590673 Sun, 01 Jan 2006 05:00:00 +0100 1590673 http://www.medworm.com/index.php?rid=1590672&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823106%26dopt%3DAbstract Authors: Silva JN, Filipe P, Morlière P, Mazière JC, Freitas JP, Cirne de Castro JL, Santus R Photodynamic therapy (PDT) by porphyrins and related tetrapyrrole derivatives is an emerging new treatment modality of tumors of lung, eosophagus and skin and of age-related macular degeneration. Phase III clinical trials for other applications such as re-stenosis after angioplasty are also underway. Under systemic conditions, the transport of porphyrin photosensitizers by serum low density lipoproteins and their specific delivery to tumor cells and vasculature is a determinant of treatment effectiveness. However, this effectiveness can be improved by increasing the selectivity of the photosensitizer uptake by tumors and by using photosensitizers absorbing light in the 660-800 nm ran... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590672 Sun, 01 Jan 2006 05:00:00 +0100 1590672 http://www.medworm.com/index.php?rid=1590671&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823107%26dopt%3DAbstract This article summarizes recent theories on the mechanisms contributing to arterial stiffness involving extracellular matrix proteins, endothelial and smooth muscle cells, cell-matrix interactions, and genetic background. Despite the important role of genetic factors in essential hypertension, little is known about the genetic of arterial stiffness. In the future, candidate genes approaches will allow to determine the cellular and molecular mechanisms of arterial stiffness. A review of different strategies aimed at lowering arterial stiffness and potentially reducing cardiovascular risk are presented. Lifestyle changes and antihypertensive drugs have showed beneficial effects in reversing stiffness. New emerging tools as gene therapy and molecules involved in matrix attachments or crosslink... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590671 Sun, 01 Jan 2006 05:00:00 +0100 1590671 http://www.medworm.com/index.php?rid=1590670&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823108%26dopt%3DAbstract Authors: Li YP, Latger-Canard V, Marchal L, Li N, Ou-Yang JP, Stoltz JF Immune homeostasis is important for the protection of a host from pathogen aggression, as well as for preventing autoimmunity. Dendritic cells (DCs), the most potent antigen presenting cells, are critical in innate, adaptive immunity and in central tolerance. Recently, their involvement in peripheral tolerance has been shown. Whether DCs induce immunity or tolerance depends on their state of maturation. Different subsets of tolerogenic DCs have been identified in vivo, either in physiological, or pathological conditions, such as tumors, or GVHD. Moreover, tolerogenic DCs can be generated in vitro, by using different culture conditions, such as IL-10 or TGF-beta. In our study, we obtained tolerogenic DCs, by culturi... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590670 Sun, 01 Jan 2006 05:00:00 +0100 1590670 http://www.medworm.com/index.php?rid=1590669&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823109%26dopt%3DAbstract Authors: Auger FA PMID: 16823109 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590669 Sun, 01 Jan 2006 05:00:00 +0100 1590669 http://www.medworm.com/index.php?rid=1590668&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823110%26dopt%3DAbstract Authors: Pulkkinen H, Tiitu V, Lammentausta E, Laasanen MS, Hämäläinen ER, Kiviranta I, Lammi MJ One goal of functional tissue engineering is to manufacture scaffolds infiltrated with chondrocytes which are suitable for transplantation into the lesion areas of articular cartilage. Various research strategies are used to fabricate cartilage transplants which would have the correct phenotype, contain enough extracellular matrix components, and have structural and biomechanical properties equivalent to normal articular cartilage. We have investigated the suitability of viscose cellulose sponges as a scaffold for cartilage tissue engineering. The sponges were tested alone, or with recombinant human type II collagen cross-linked inside the material. Scanning electron microsco... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590668 Sun, 01 Jan 2006 05:00:00 +0100 1590668 http://www.medworm.com/index.php?rid=1590667&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823111%26dopt%3DAbstract Authors: Stoltz JF, Bensoussan D, Decot V, Ciree A, Netter P, Gillet P Most human tissues do not regenerate spontaneously; this is why cell therapies and tissue engineering are promising alternatives. The principle is simple: cells are collected in a patient and introduced in the damaged tissue or in a tridimentional porous support and harvested in a bioreactor in which the physico-chemical and mechanical parameters are controlled. Once the tissues (or the cells) are mature they may be implanted. In parallel, the development of biotherapies with stem cells is a field of research in turmoil given the hopes for clinical applications that it brings up. Embryonic stem cells are potentially more interesting since they are totipotent, but they can only be obtained at the very early stages of... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590667 Sun, 01 Jan 2006 05:00:00 +0100 1590667 http://www.medworm.com/index.php?rid=1590666&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823112%26dopt%3DAbstract Authors: Schumann D, Kujat R, Nerlich M, Angele P Articular cartilage possesses little capacity for endogenous repair after having been damaged by disease or trauma. Various surgical procedures depending on ingrowth of mesenchymal stem cells into the defects showed repair with fibrocartilage which is of minor quality and less resistant against physical forces. New treatment options using Tissue Engineering strategies for cartilage repair showed intriguing results. Human mesenchymal stem cells (MSC) isolated from bone marrow are becoming increasingly recognized for their potential to generate different cell types and thereby function effectively in vitro or in vivo in tissue repair. Incorporation of MSCs in suitable tissue engineering scaffolds and culture in chondrogenic medium can pro... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590666 Sun, 01 Jan 2006 05:00:00 +0100 1590666 http://www.medworm.com/index.php?rid=1590665&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823113%26dopt%3DAbstract Authors: Amédée J, Guillotin B, Pallu S, Bareille R, Guignandon A, Bourget Ch, Durrieu MC, Bordenave L In the field of osseous substitution, the possibilities being offered to the surgeons prove sometimes difficult to apply in particular in the case of great losses of osseous substance. For these reasons, it is necessary to develop innovative techniques to satisfy the request increasing for substitutes and to see appearing on the market solutions combining availability, perenniality and biosecurity of the implants. The implantation of stem cells in a biomaterial opens a way of development of therapeutic substitute. Moreover, in order to optimize the rehabitation of the biomaterials by the cells and the host tissues, the second approach consists in modifying the surface of mat... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590665 Sun, 01 Jan 2006 05:00:00 +0100 1590665 http://www.medworm.com/index.php?rid=1590664&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823114%26dopt%3DAbstract Authors: Matousková E, Broz L, Stolbová V, Klein L, Konigová R, Veselý P The tissue engineered skin should be composed of both dermal and epidermal layers. We combined cultured human allogeneic keratinocytes with acellular xenodermis prepared from pig xenografts. The resulting composite skin was termed recombined human/pig skin (RHPS), and could be cultured in both, undifferentiated and differentiated phenotype. The undifferentiated RHPS was grown submerged and formed 1-2 layers of keratinocytes. The differentiated phenotype (D-RHPS) was grown at the air-liquid interface and formed 5-20 cell layers similar to the normal epidermis, including the granular and horny layers. Undifferentiated RHPS has skin-like consistency and has been successfully used for treatment of ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590664 Sun, 01 Jan 2006 05:00:00 +0100 1590664 http://www.medworm.com/index.php?rid=1590663&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823115%26dopt%3DAbstract Authors: Auxenfans C, Colloud M, Debard AL, Braye FM, Amini M, Allombert-Blaise V, Builles N, Claudy A, Damour O The clinical interest of using allogenic epidermal sheets (AES) has largely been shown [1,2,3]. As well as covering, they also stimulate healing, by simultaneously secreting numerous growth factors (GFs), although little is known on their mechanism of action. Our objectives were to: (a) devise a test for the efficacy of AES release, (b) select keratinocyte-secreting strains and optimal culture conditions. Three GFs were selected: IL-1alpha, IL-8 and VEGF. Three different keratinocyte strains were cultured for 3 and 6 days after confluence for 3 passages. Assays were performed after 3 h and 24 h+3 h after dispase treatment (AES conservation for 24 h then change of medium and ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590663 Sun, 01 Jan 2006 05:00:00 +0100 1590663 http://www.medworm.com/index.php?rid=1590662&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823116%26dopt%3DAbstract Authors: Dezutter-Dambuyant C, Black A, Bechetoille N, Bouez C, Maréchal S, Auxenfans C, Cenizo V, Pascal P, Perrier E, Damour O The development of human skin models that have the same properties as genuine human skin is of particular significance. Very promising skin models are the three-dimensional artificial skin constructs, which, similar to genuine skin, consist of an epidermis of differentiated keratinocytes and a dermis. A skin equivalent based on a collagen-glycosaminoglycan-chitosan dermal substrate has been developed to meet the growing demand in tissue engineered skin equivalents. We used this model to investigate whether CD34-generated Langerhans/dendritic cell precursors could be integrated into this skin equivalent model and pursue their differentiation without addit... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590662 Sun, 01 Jan 2006 05:00:00 +0100 1590662 http://www.medworm.com/index.php?rid=1590661&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16823117%26dopt%3DAbstract Conclusion: We selected the following medium, which provides optimal growth and preservation of the CD34+ phenotype: DMEM/HAM-F12 + 10% NCS + 5 ng/ml b-FGF + antibiotics. PMID: 16823117 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590661 Sun, 01 Jan 2006 05:00:00 +0100 1590661 http://www.medworm.com/index.php?rid=1590660&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16971740%26dopt%3DAbstract Authors: Du RL, Chang J To evaluate the influence of Zn on the formation of hydroxyapatite (HA), zinc-containing glass 58S4Z was prepared by substitution of CaO with 4 wt% ZnO in 58S bioactive glass, and Zn-containing simulated body fluid (ZnSBF) was prepared as soaking fluid. During the soaking, all samples could induce the deposition of HA, but 58S4Z in SBF and 58S in ZnSBF showed lower formation rate of HA than 58S in SBF, which suggests that both the Zn in glass and the Zn2+ in soaking fluid retarded the deposition of HA. The ICP and EDS analysis results indicated that the main factor was the preferential reaction of Zn2+ with PO4(3-), which resulted in a decrease of the PO4(3-) concentration in soaking fluid and the nucleation of HA. Besides the inhibitory effect of Zn2+ in the so... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590660 Sun, 01 Jan 2006 05:00:00 +0100 1590660 http://www.medworm.com/index.php?rid=1590659&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16971741%26dopt%3DAbstract Authors: Gray GB, Kataria V, McManus S, Jagger DC The aim of this study was to investigate the effect of one step adhesives on the shear bond strength of a compomer restorative material to both enamel and dentine. Human extracted teeth were used for the study. Ten samples were prepared for both enamel and dentine specimens for each of the five groups: Tooth, no etch, Prime and Bond NT (P+B NT); tooth, Non-Rinse Conditioner (NRC), P+B NT; tooth, NRC, Prime and Bond 2.1 (P+B 2.1); tooth, etch, P+B NT; tooth, etch, P+B 2.1. The specimens were subjected to bond testing. The shear bond strength was measured using an Inston 1193 testing machine using a cross head speed of 1 mm/minute. The specimens were tested to destruction. The results show that for the enamel specimens the highest bond st... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590659 Sun, 01 Jan 2006 05:00:00 +0100 1590659 http://www.medworm.com/index.php?rid=1590658&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16971742%26dopt%3DAbstract Authors: Ozeki K, Okuyama Y, Fukui Y, Aoki H Hydroxyapatite (HA) was coated onto titanium implants using radio frequency magnetron sputtering. The HA films were crystallized in an autoclave tube using low temperature hydrothermal treatment. The average film thickness on the implant was 1.1 microm. HA-coated and pure-titanium implants were inserted into canine mandibles for up to 24 weeks. Forty-eight implants were placed in eight beagles. After 2, 4, 12 and 24 weeks, implants were retrieved and prepared for histological observation, and the HA film thickness was determined using energy-dispersive X-ray spectroscopy. Light microscopy revealed that, after two weeks, the bone response to the HA-coated implants was much better than to the pure titanium implants, and osteoblasts were observ... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590658 Sun, 01 Jan 2006 05:00:00 +0100 1590658 http://www.medworm.com/index.php?rid=1590657&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16971743%26dopt%3DAbstract Authors: Beaupied H, Dupuis A, Arlettaz A, Brunet-Imbault B, Bonnet N, Jaffré C, Benhamou CL, Courteix D The bone samples used in clinical and experimental trials must be the less damaged as possible to avoid alterations of their properties. However, the mode of storage might possibly alter the bone properties, particularly microarchitecture and strength. The aim of our study was to analyze the effects of deep-freezing and alcohol conservation techniques on the densitometric, microarchitectural and biomechanical parameters of rat femurs. The left femurs were elongated in uniaxial tension up to breakdown in order to calculate biomechanical parameters. The densitometric and microarchitectural properties of right femurs were evaluated using dual-energy X-ray absorptiometry and microc... Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590657 Sun, 01 Jan 2006 05:00:00 +0100 1590657 http://www.medworm.com/index.php?rid=1590656&cid=s_37582_173_f&fid=37582&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D16971744%26dopt%3DAbstract This study implies that an instrumented spine with integrity of posterior complex is less likely to develop adjacent instability than a spine with destruction of the anchoring point for supraspinous ligament. PMID: 16971744 [PubMed - indexed for MEDLINE] (Source: Bio-Medical Materials and Engineering) Bio-Medical Materials and Engineering journals http://www.medworm.com/rss/comments.php?id=1590656 Sun, 01 Jan 2006 05:00:00 +0100 1590656