MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Biochemical Genetics' source. Thu, 09 Feb 2012 09:43:28 +0100 http://www.medworm.com/index.php?rid=5635672&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22273846%26dopt%3DAbstract Authors: Huan HV, Wang Y, Zhang S Abstract The coumarate:coenzyme A ligase 1 gene (4CL1) was isolated from secondary developing xylem tissues of Pinus massoniana Lamb (Pm4CL1). The Pm4CL1 gene was inserted into a pQE30 expression vector in both sense and antisense orientations to form a pQE30-4CL1-anti4CL1. The expression level of the Pm4CL1 gene in Escherichia coli cells was inhibited by the antisense mRNA of the Pm4CL1 gene. The results of reverse transcriptase polymerase chain reaction and sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the average mRNA and protein expression levels of the Pm4CL1 gene in E. coli cells were decreased by 43 and 46%, respectively. These results are an important basis for further investigation. PMID: 22273846 [PubMed - as... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5635672 Wed, 25 Jan 2012 05:00:00 +0100 5635672 http://www.medworm.com/index.php?rid=5635673&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22270475%26dopt%3DAbstract Authors: Wan Y, Yang S, Sun F, Wang J, Chen Q, Hong A Abstract The alkaline phosphatase (ALP) gene is an important marker of osteoblast differentiation and bone formation. Although the molecular mechanisms of increased ALP expression in response to all-trans retinoic acid (ATRA) have been reported, the role of ATRA in chromatin structure changes remains unknown. Our results show that the expression of mouse liver, bone, and kidney ALP (mL/B/K-ALP) induced by ATRA in C3H10T 1/2 cells was related to the retinoic acid nuclear receptors, RARα and RARβ, which are not involved in the MAPK pathway. DNase I hypersensitivity analysis revealed an inducible hypersensitive site in the mL/B/K-ALP promoter at ~520 bp upstream of the transcription start site. Chromatin immunoprecipitation expe... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5635673 Sun, 22 Jan 2012 05:00:00 +0100 5635673 http://www.medworm.com/index.php?rid=5635674&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22270474%26dopt%3DAbstract Authors: Saha S, Basu T Abstract When leu (-) auxotrophs of Escherichia coli, after UV irradiation, were grown at temperatures between 30 and 47°C, the frequency of UV-induced mutation from leu (-) to leu (+) revertant increased as the UV dose and the temperature increased. For cells exposed to a UV dose of 45 J/m(2), the mutation frequency at 47°C was 1.9 times that at 30°C; for a dose of 90 J/m(2), it was 3.25 times; and for 135 J/m(2), it was 4.8 times. Similar enhancement of reversion frequency was observed when the irradiated cells were grown at 30°C in the presence of a heat shock inducer, ethanol (8% v/v). Heat shock-mediated enhancement of UV mutagenesis did not occur in an E. coli mutant sigma 32 (heat shock regulator protein), but sigm... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5635674 Sat, 21 Jan 2012 05:00:00 +0100 5635674 http://www.medworm.com/index.php?rid=5617473&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22258312%26dopt%3DAbstract Authors: Strychalski J, Czarnik U, Zabolewicz T Abstract Allele and haplotype segregation at the polymorphic sites within the promoter (23indel) and intron 1 (12indel) regions of the PRNP gene was analyzed in Polish Holstein-Friesian cattle. More 23del/del homozygotes and fewer 23ins/ins homozygotes than expected were observed in the offspring of ♂ 23ins/del × ♀ 23ins/del parents. In the offspring of ♂ 23ins/del × ♀ 23del/del parents and ♂ 23del/del × ♀ 23ins/del parents, a trend toward more 23del/del animals and fewer 23ins/del animals than expected was noted. At the 12indel polymorphic site, the only trend found was one toward fewer 12ins/ins genotypes and more 12ins/del and 12del/del genotypes than expected in the offspring of ♂ 12ins/del × ♀ 12ins/de... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5617473 Thu, 19 Jan 2012 05:00:00 +0100 5617473 http://www.medworm.com/index.php?rid=5573580&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22212460%26dopt%3DAbstract Analysis of p15 ( INK4b ) and p16 ( INK4a ) Gene Methylation in Patients with Oral Squamous Cell Carcinoma. Biochem Genet. 2012 Jan 3; Authors: Kordi-Tamandani DM, Ladies MA, Hashemi M, Moazeni-Roodi AK, Krishna S, Torkamanzehi A PMID: 22212460 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5573580 Tue, 03 Jan 2012 05:00:00 +0100 5573580 http://www.medworm.com/index.php?rid=5573582&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22210543%26dopt%3DAbstract Authors: Zhou TH, Dong SS, Li S, Zhao GF Abstract The endangered perennial plant Saruma henryi Oliv. is endemic to China and has phylogenetic, ecological, and medicinal value. We used 10 microsatellite (SSR) loci to investigate genetic diversity and differentiation in 16 natural populations. Genetic diversity was high at the species level (H (E) = 0.9427, h = 0.9410, I = 3.0213) and low at the population level (H (E) = 0.4441, h = 0.4307, I = 0.6822). Pronounced genetic differentiation was detected among populations (G (ST) = 0.5428, F (ST) = 0.5524), in line with the limited among-population gene flow (N (m) = 0.21). The significant isolation-by-distance pattern revealed by a Mantel test (r = 0.311, P = 0.001... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5573582 Sun, 01 Jan 2012 05:00:00 +0100 5573582 http://www.medworm.com/index.php?rid=5573581&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22210544%26dopt%3DAbstract Authors: Wang L, Wei Y Abstract Ferritin is a ubiquitous and conserved iron storage protein that plays a central role in iron metabolism. The ferritin heavy chain subunit (FerH) homolog was isolated from yellow grouper (Epinephelus awoara) spleen using suppression subtractive hybridization and RACE-PCR. The nucleotide sequence of FerH full-length cDNA was 1173 bp and contained an open reading frame of 534 bp, encoding a putative protein of 177 amino acids. The encoded protein shows 78-94% identity with homologs. Based on phylogenetic analysis, yellow grouper FerH is highly conserved throughout evolution and is closer to European seabass than to other species. RT-PCR analysis demonstrated that FerH was widely expressed in various healthy tissues and significantly up-regulated in l... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5573581 Sun, 01 Jan 2012 05:00:00 +0100 5573581 http://www.medworm.com/index.php?rid=5557543&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22205502%26dopt%3DAbstract Authors: Larsen PF, Nielsen EE, Meier K, Olsvik PA, Hansen MM, Loeschcke V Abstract Populations of marine fish, even from contrasting habitats, generally show low genetic differentiation at neutral genetic markers. Nevertheless, there is increasing evidence for differences in gene expression among populations that may be ascribed to adaptive divergence. Studying variation in salinity tolerance and gene expression among Atlantic cod (Gadus morhua) from two populations distributed across a steep salinity gradient, we observed high mortality (45% North Sea cod and 80% Baltic Sea cod) in a reciprocal common garden setup. Quantitative RT-PCR assays for expression of hsp70 and Na/K-ATPase α genes demonstrated significant differences in gene regulation within and between populations and ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5557543 Thu, 29 Dec 2011 05:00:00 +0100 5557543 http://www.medworm.com/index.php?rid=5543580&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22193288%26dopt%3DAbstract Authors: Schlegel M, Ali HS, Stieger N, Groschup MH, Wolf R, Ulrich RG PMID: 22193288 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5543580 Fri, 23 Dec 2011 05:00:00 +0100 5543580 http://www.medworm.com/index.php?rid=5523352&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179844%26dopt%3DAbstract Authors: Arias-Pérez A, Fernández-Tajes J, Gaspar MB, Méndez J Abstract The sword razor shell Ensis siliqua (Linnaeus, 1758) is found mainly from Norway to the Atlantic coast of the Iberian Peninsula. It is intensively caught in Europe, being highly appreciated as seafood. To help in its conservation and management, five microsatellite markers were isolated and genetic variation was analyzed in samples from Ireland, Spain, and Portugal. The highly significant differentiation (θ = 0.287, P < 0.001) observed was mainly due to differences between samples from Irish and Iberian Peninsula localities, except Aveiro (its sample resembled the Irish samples, and it may be predominantly self-recruiting). These groups of samples showed significant differences in allelic richness th... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523352 Sat, 17 Dec 2011 05:00:00 +0100 5523352 http://www.medworm.com/index.php?rid=5523351&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22179845%26dopt%3DAbstract This study supported that prediction, as a RAPD-based analysis of molecular variance revealed a high degree of genetic structure (Φ (st) = 0.157, P = 0.001) and low gene flow (N (m) = 1.0) among four central populations of Pinguicula moranensis in Mexico, with a maximum geographic separation of about 140 km. The four populations also exhibited high levels of genetic diversity (mean Nei's genetic diversity = 0.3716; % polymorphism = 95.45%). The evolutionary implications of the genetic structure found in P. moranensis for other species in the genus are discussed in the context of the naturally fragmented distribution and a set of life history traits shared by most Pinguicula species that could promote geographic isolation and limited gene flow. PMID: 2217... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523351 Sat, 17 Dec 2011 05:00:00 +0100 5523351 http://www.medworm.com/index.php?rid=5523353&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22173629%26dopt%3DAbstract Authors: Palabiyik B, Kig C, Pekmez M, Dalyan L, Arda N, Temizkan G Abstract The invertase mutant defective in the glucose signaling pathway of Schizosaccharomyces pombe (ird11) is resistant to glucose repression. This mutant is able to consume sucrose alongside glucose and grows in glucose-containing media with a generation time close to that of the wild type. Intracellular oxidation, protein carbonyl, and reduced glutathione levels and catalase, superoxide dismutase, and glutathione peroxidase activity were investigated in ird11, to determine the relationship between oxidative stress response and glucose signaling. The expression profiles of some genes involved in regulation of glucose repression (fbp1, fructose-1,6-bis-phosphatase; hxk2, hexokinase) and stress response (atf1 and... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523353 Fri, 16 Dec 2011 05:00:00 +0100 5523353 http://www.medworm.com/index.php?rid=5523354&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22170480%26dopt%3DAbstract In this study, we identified porcine single nucleotide polymorphisms (SNPs) by aligning eight sequences generated with two approaches: amplification of 665 intronic regions using one sample from each of eight breeds, including three East Asian pigs, and amplification of 289 3'-UTR regions using two samples from each of four major commercial breeds. The 1,760 and 599 SNPs were validated using two 384-sample DNA panels by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The phylogenetic tree and Structure analyses classified the pigs into two large clusters: Euro-American and East Asian populations. The membership proportions, however, differed between inferred clusters for K = 2 generated by the two approaches. With intronic SNPs, Euro-American breeds constitu... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523354 Thu, 15 Dec 2011 05:00:00 +0100 5523354 http://www.medworm.com/index.php?rid=5523355&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22161014%26dopt%3DAbstract Authors: Du M, Liang Z, Xu X, Li J, Ai H, Zhang J, Zhang S PMID: 22161014 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523355 Sat, 10 Dec 2011 05:00:00 +0100 5523355 http://www.medworm.com/index.php?rid=5523357&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160248%26dopt%3DAbstract Authors: Xie Z, Neigel J, Chlan C Abstract Two different but related sequences that encode Vigna luteola 7S vicilins were isolated and characterized. The sequences differ by two nucleotide substitutions, each of which results in an amino acid replacement. This low level of divergence suggests that a recent gene duplication has occurred. Both variants are expressed in cDNA populations; therefore, neither gene is a pseudogene. Both copies were present in all individuals (72) analyzed using real-time PCR and TaqMan probes. Segregation was not observed. The two sequences are not independent alleles. Vicilin genomic sequences of 11 specimens from six geographic locations were determined. No polymorphic sites were identified in either of the two gene copies. This lack of polymorphism sug... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523357 Fri, 09 Dec 2011 05:00:00 +0100 5523357 http://www.medworm.com/index.php?rid=5523356&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22160249%26dopt%3DAbstract This study investigates aromatase gene polymorphism, which might influence bone strength in terms of mineral density and quality. We explored the relationship between CYP19 polymorphisms and vertebral fractures in postmenopausal Japanese women. In addition, we compared estrogen and testosterone levels in Japanese postmenopausal women with and without fractures. Osteoporotic postmenopausal women showed higher incidences of vertebral fractures than osteopenic women or women with normal lumbar bone mineral density (L2-4 BMD). Estrogen concentrations in postmenopausal women were associated with BMD; however, no association was found between sex hormone levels and the presence of fractures. The C allele rs2470152 was significantly associated with increased risk of vertebral fractures (P = 0.0... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523356 Fri, 09 Dec 2011 05:00:00 +0100 5523356 http://www.medworm.com/index.php?rid=5523358&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22139460%26dopt%3DAbstract Authors: Zhou R, Li Y, Li JQ, Liu NF Abstract Seasonal changes in genetic diversity of Meriones meridianus and Dipus sagitta populations in the Ningxia Hui Autonomous Region were monitored using inter-simple sequence repeats. There were 45 discernible DNA fragments isolated from 33 samples of M. meridianus, and 25 from 40 samples of D. sagitta. The differences in gene frequencies are statistically significant for some alleles. Nei's index revealed that the greatest genetic differentiation was distributed within the seasonal groups. Nei's and Shannon's indexes exhibited similar seasonal trends, indicating that the spring group has the highest level of genetic diversity and that genetic diversity changes with the seasons. Correlation analysis found no statistically significant correl... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523358 Sun, 04 Dec 2011 05:00:00 +0100 5523358 http://www.medworm.com/index.php?rid=5523359&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22119863%26dopt%3DAbstract Authors: Sin O, Mabiala P, Liu Y, Sun Y, Hu T, Liu Q, Guo D Abstract Artificial microRNA (miRNA) expression vectors have been developed and used for RNA interference. The secondary structure of artificial miRNA is important for RNA interference efficacy. We designed two groups of six artificial splicing miRNA 155-based miRNAs (SM155-based miRNAs) with the same target in the coding region or 3' UTR of a target gene and studied their RNA silencing efficiency and interferon β (IFN-β) induction effects. SM155-based miRNA with a mismatch at the +1 position and a bulge at the +11, +12 positions in a miRNA precursor stem-loop structure showed the highest gene silencing efficiency and lowest IFN-β induction effect (increased IFN-β mRNA level by 10% in both target cases), regardless of ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523359 Sun, 27 Nov 2011 05:00:00 +0100 5523359 http://www.medworm.com/index.php?rid=5523360&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22113854%26dopt%3DAbstract This study investigates potential associations between CD36 gene variants and the presence of risk factors in Caucasians with coronary artery disease (CAD) manifested at a young age. The study group consisted of 90 patients; the men were ≤ 50 years old and the women were ≤ 55 years old. Amplicons of exons 4 and 5 including fragments of introns were analyzed by DHPLC. Two polymorphisms were found: IVS3-6 T/C (rs3173798) and IVS4-10 G/A (rs3211892). The C allele of the IVS3-6 T/C polymorphism was associated with higher prevalence of obesity and diabetes, higher hsCRP, lower Lp(a) serum concentrations, and younger age at myocardial infarction. The A allele of the IVS4-10 G/A polymorphism was associated with older age of myocardial infarction and higher white blood cell count. The fu... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5523360 Thu, 24 Nov 2011 05:00:00 +0100 5523360 http://www.medworm.com/index.php?rid=5433023&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22089542%26dopt%3DAbstract Authors: Yang Y, Wang LL, Li YH, Gao XN, Liu Y, Yu L Abstract To test the hypothesis that methylation of a CpG island is associated with regulation of microRNA expression, we investigated CpG islands in the upstream sequences of microRNA precursors (pre-miRNAs) through bioinformatic analysis and determined whether the CpG islands were methylated by methylation-specific PCR in the k-562 cell line. We used 5-azacytidine for DNA demethylation, and changes in microRNA expression were detected by microarray assay, RT-PCR, and real-time PCR after 5-azacytidine induction. We showed that the CpG islands in the upstream regions of 18 pre-miRNAs were methylated, including miR-663, miR-369, miR-615, and miR-410, and promoter activity was detected in the upstream region of pre-miR-663. We foun... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5433023 Thu, 17 Nov 2011 05:00:00 +0100 5433023 http://www.medworm.com/index.php?rid=5433022&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22089543%26dopt%3DAbstract Authors: Keong BP, Harikrishna JA Abstract A preliminary screening was conducted on BC(3)F(1) and BC(4)F(1) backcross families developed from crossing Oryza sativa (MR219) and O. rufipogon (IRGC105491). Despite earlier results showing that O. rufipogon alleles (wild introgression) contributed to both number of panicles (qPPL-2) and tillers (qTPL-2) at loci RM250, RM208, and RM48 in line A20 of the BC(2)F(2) population, we observed that wild introgression was lost at loci RM250 and RM208 but retained at locus RM48 in BC(3)F(1) and BC(4)F(1). Progeny tests conducted utilizing genotype and phenotype data on both BC(4)F(1) and a reference population, BC(2)F(7) (A20 line), did not show significant differences between groups having the MR219 allele and wild introgression at locus RM48. T... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5433022 Thu, 17 Nov 2011 05:00:00 +0100 5433022 http://www.medworm.com/index.php?rid=5338089&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21983932%26dopt%3DAbstract This study investigated the association of hepatocyte nuclear factor 4 (HNF4) alpha single nucleotide polymorphisms (SNPs) with type 2 diabetes with or without metabolic syndrome in Malaysia. Nine HNF4 alpha SNPs were genotyped in 390 type 2 diabetic subjects with metabolic syndrome, 135 type 2 diabetic subjects without metabolic syndrome, and 160 control subjects. The SNPs rs4810424, rs1884613, and rs2144908 were associated with protection against type 2 diabetes without metabolic syndrome (recessive P = 0.018, OR 0.32; P = 0.004, OR 0.25; P = 0.005, OR 0.24, respectively). The 6-SNP haplotype2 CCCGTC containing the risk genotype of these SNPs was associated with higher risk for type 2 diabetes with or without metabolic syndrome (P = 0.002, OR 2.2; P = 0.004, OR 3.1). These data... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5338089 Sun, 09 Oct 2011 04:00:00 +0100 5338089 http://www.medworm.com/index.php?rid=5338090&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21979450%26dopt%3DAbstract Authors: Lan XY, Liu JB, Zhu JL, Liu TG, Zhang LZ, Zhang Y, Lei CZ, Chen H PMID: 21979450 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5338090 Fri, 07 Oct 2011 04:00:00 +0100 5338090 http://www.medworm.com/index.php?rid=5293075&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21964518%26dopt%3DAbstract Authors: Miyamoto H Abstract Carbonic anhydrases are conserved in vertebrates and invertebrates, and a noncatalytic carbonic anhydrase-related protein VIII (CARP VIII) has been found in deuterostomes and the phylum Placozoa. I isolated a cDNA encoding a noncatalytic CARP from the mantle of the pearl oyster Pinctada fucata. The polypeptide (CARP-1) predicted from the nucleotide sequence shares 44-60% identity with known CARP VIII sequences, and its phylogenetic analysis showed that P. fucata formed a single group with deuterostome invertebrates. However, since CARP VIII sequences are not identified in protostomes, these results suggest that CARP-1 may have originated in molluscs independently from deuterostome CARP VIII sequences. PMID: 21964518 [PubMed - as supplied by publishe... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5293075 Sat, 01 Oct 2011 04:00:00 +0100 5293075 http://www.medworm.com/index.php?rid=5293074&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21964519%26dopt%3DAbstract This study determined the full-length sequence of CYP2E1, one of six cytochrome P450 genes previously examined in camel tissues by western blotting and semi-quantitative PCR. The Camelus dromedarius CYP2E1 has an open reading frame of 1,473 bp, and the cDNA encodes a protein of 490 amino acid residues with a molecular weight of 54.8 kDa. The deduced amino acid sequence showed the highest identity with Bos taurus (88%), Sus scrofa (87%), and Homo sapiens (83%). In a phylogenetic analysis, the C. dromedarius CYP2E1 isoform was located beside cattle and pigs. The deduced amino acid sequence of camel CYP2E1 showed the conserved proline-rich amino terminus and the heme-binding signature localized near the carboxy terminus of the protein. PMID: 21964519 [PubMed - as supplied by publisher] ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5293074 Sat, 01 Oct 2011 04:00:00 +0100 5293074 http://www.medworm.com/index.php?rid=5293073&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21964520%26dopt%3DAbstract Authors: Kurosaki T, Gojobori J, Ueda S Abstract Human PQBP-1 is known to interact with triplet repeat disease gene products such as ataxin and huntingtin through their poly-glutamine (poly-Q) tracts. The poly-Q tracts show extensive variation in both the number and the configuration of repeats among species. A surface plasmon resonance assay showed clear interaction between human PQBP-1 and Q(11), representative of the poly-Q tract of the ataxin-1 of Old World monkeys. No response was observed using Q(2)PQ(2)P(4)Q(2), representative of the poly-Q tract of the ataxin-1 of New World monkeys. This implies that the interaction of human PQBP-1 with ataxin-1 is limited to humans and closely related species. Comparison of the human and mouse PQBP-1 sequences showed an elevated amino acid... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5293073 Sat, 01 Oct 2011 04:00:00 +0100 5293073 http://www.medworm.com/index.php?rid=5271498&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21956797%26dopt%3DAbstract In this study, we screened mutations in the complete coding region of the porcine B3GNT5 gene and identified four SNPs in the 3' untranslated regions. We genotyped the four SNPs across a large-scale White Duroc × Chinese Erhualian F (2) resource population (total F (2) = 755) and 292 purebred piglets representing 15 Chinese and Western breeds. We found that the g.1476G→A locus and haplotypes [A;T;G;T] and [A;G;G;T] had significant association with susceptibility to ETEC F4ac in the resource population. None of the B3GNT5 polymorphisms and haplotypes was associated with susceptibility to ETEC F4ab/ac in outbred piglets. This result, together with other reports, supports the conclusion that B3GNT5 is not the responsible gene encoding the ETEC F4ab/ac receptors. ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5271498 Thu, 29 Sep 2011 04:00:00 +0100 5271498 http://www.medworm.com/index.php?rid=5271503&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21952874%26dopt%3DAbstract Na(+)/H (+) Exchanger 1 Gene Expression in Tissues of Yellow Chicken. Biochem Genet. 2011 Sep 28; Authors: Ning ZY, An YF, Qi WB, Wang H, Pan JQ, Wu XT, Liao M Abstract The Na(+)/H(+) exchanger 1 (NHE1) transmembrane protein regulates intracellular pH, cell survival, cell growth, cell differentiation and plays a critical role in the progression of some diseases, including the pathogenesis of J avian leukosis. The chicken is an ideal model to study the function of NHE1 because it has developed highly efficient Na(+)-absorptive mechanisms in its small and large intestines. To date, there has been no detailed expression analysis to determine NHE1 expression in various tissues of the chicken. We determined the mRNA and protein expression levels of avian NHE1 by real-time qu... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5271503 Wed, 28 Sep 2011 04:00:00 +0100 5271503 http://www.medworm.com/index.php?rid=5271502&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21952875%26dopt%3DAbstract Authors: Dong C, Zhang J, Qiao J, He G Abstract A newly discovered melanopsin gene (Opn4) encodes a member of the opsins, melanopsin. Two melanopsin genes, mammalian-like Opn4m and Xenopus-like Opn4x, have been described in nonmammalian vertebrates, but the underlying evolutionary mechanisms behind the duplication of melanopsin genes remain unclear. We conducted a comprehensive evolutionary analysis within a phylogenetic framework. In our phylogenetic tree, the duplication of Opn4m and Opn4x probably occurred prior to the emergence of vertebrates, and subsequently Opn4x disappeared in the lineages leading to mammalian species. Evolutionary analyses show strong purifying selection during melanopsin evolution. We also provide evidence that Opn4x underwent positive selection after the... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5271502 Wed, 28 Sep 2011 04:00:00 +0100 5271502 http://www.medworm.com/index.php?rid=5271501&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21952876%26dopt%3DAbstract In this study, we attempt to evaluate the etiological role of eight MLH1 missense variants. The variants were analyzed for their ability to affect MLH1 protein interaction with its partner PMS2 in vivo employing a yeast two-hybrid system. In addition, a SIFT (sorting intolerant from tolerant) algorithm was adopted to predict the effects of amino acid substitutions. Finally, scanning of mutations in a normal Chinese population and assay of the clinical characteristics have all been taken into account. Our results demonstrated that the MLH1 variants D485E and L653R cause functional alterations of the human MutLα complex significantly. The R265C, D304V, A586P, and R755S variants affect partial interaction. The remaining two variants, N38D and L559R, could be nonfunctional polymorphisms or mi... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5271501 Wed, 28 Sep 2011 04:00:00 +0100 5271501 http://www.medworm.com/index.php?rid=5271500&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21952877%26dopt%3DAbstract Authors: Tsunoda K, Harihara S, Tanabe Y, Dashnyam B Abstract Allele frequencies at six RFLP sites (Ins/Del, ApaLI, AluI, XbaI, MspI, and EcoRI) of the apolipoprotein B gene (APOB) and the relationship of genotypes with plasma lipid and lipoprotein levels in the Mongolian Buryat were investigated. Common alleles at these sites in 110 Buryat subjects were I, G, A-, X-, M+, and E+; the frequencies of 0.809-0.991 differed strikingly from those of a few Asians and most Europeans. Five unambiguous haplotypes of all sites were revealed at 74%; haplotype IGA-X-M+E+ (000000) was the most frequent (67%), followed by IGA+X-M+E+ (001000) (19%). The frequency constitution differed significantly from the Chinese, Malaysians, and Caucasians but resembled the Indians. No APOB polymorphi... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5271500 Wed, 28 Sep 2011 04:00:00 +0100 5271500 http://www.medworm.com/index.php?rid=5271499&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21952878%26dopt%3DAbstract This study was designed to analyze variation of ovine prion protein in sheep breeds in Iran and Turkey. A competitive approach was used to analyze the open reading frame (ORF) of the ovine PRNP gene using a total of 186 samples from five indigenous sheep breeds. The ARQ allele was found to be the predominant allele in five breeds. The ARR allele was not observed in homozygous combination among the 11 genotypes found in the study. In addition, six other polymorphisms were indicated. These findings have great significance for estimating genetic variability in the PRNP gene with regard to Iranian and Turkish sheep. Since no information on the susceptibility of some genotypes identified in this study has been reported, no grouping was made on the basis of susceptibility. PMID: 21952878 [Pu... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5271499 Wed, 28 Sep 2011 04:00:00 +0100 5271499 http://www.medworm.com/index.php?rid=5271504&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21948269%26dopt%3DAbstract This study was undertaken to detect polymorphisms in the goat and sheep mannose-binding lectin encoding gene (MBL2) and to explore allelic variability of this gene in these two species. The analysis and comparison of the sequences obtained from sheep showed 13 polymorphic sites, six in the promoter and seven in exon 1, four of which were of the missense type. In the goats, 12 polymorphic sites were detected, five intronic, five in the promoter, and one exonic. The exon site was responsible for an amino acid change. Mutations detected at the MBL2 locus in the sheep are of particular interest, being potentially responsible for the alterations of gene expression. A population survey involved 102 ewes of the Sardinian breed and 218 goats of the Nicastrese breed, all reared in southern Italy. ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5271504 Sat, 24 Sep 2011 04:00:00 +0100 5271504 http://www.medworm.com/index.php?rid=5271505&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21948254%26dopt%3DAbstract Authors: Ru B, Han N, He G, Brayer K, Zhang S, Wang Z Abstract GJB6 plays a crucial role in hearing. In mammals, bats use ultrasonic echolocation for orientation and locating prey. To investigate the evolution of GJB6 in mammals, we cloned the full-length coding region of GJB6 from 16 species of bats and 4 other mammal species and compared them with orthologous sequences in 11 other mammals. The results show purifying selection on GJB6 in mammals, as well as in the bat lineage, which indicates an important role for GJB6 in mammal hearing. We also found one unique amino acid substitution shared by 16 species of bats and 10 shared by two species of artiodactyls. This positioned the artiodactyls at an abnormal location in the gene tree. In addition, the cytoplasmic loop and carboxy te... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5271505 Fri, 23 Sep 2011 04:00:00 +0100 5271505 http://www.medworm.com/index.php?rid=5255721&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21938561%26dopt%3DAbstract Authors: Fernandes CR, Martins CF, Ferreira KM, Del Lama MA Abstract Gene variation and the differentiation of two populations of Partamona seridoensis (Hymenoptera: Apidae: Meliponini) from the Caatinga biome, a semiarid ecosystem unique to Brazil, were estimated through allozymic and microsatellite analyses. These populations exhibited similar low degrees of enzyme gene variation. Observed genotype frequencies at the allozyme and microsatellite loci were in accordance with Hardy-Weinberg equilibrium in the two populations. Both markers demonstrated that the two populations are not genetically homogeneous and must be considered distinct populations. The occurrence of private alleles at the allozyme and microsatellite loci corroborates this differentiation, sustaining the hypothesi... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5255721 Thu, 22 Sep 2011 04:00:00 +0100 5255721 http://www.medworm.com/index.php?rid=5255720&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21938562%26dopt%3DAbstract Authors: Abdul Muneer PM, Gopalakrishnan A, Musammilu KK, Basheer VS, Mohindra V, Lal KK, Padmakumar KG, Ponniah AG Abstract The comparative assessment of genetic diversity using allozymes, random amplified polymorphic DNA (RAPD), and microsatellite markers was conducted in endemic and endangered yellow catfish (Horabagrus brachysoma) sampled from three locations in Western Ghats river systems of India. Among the three markers, microsatellites show more polymorphism, having 100% polymorphic loci, whereas allozymes show the least (56%). In RAPD, 60.5% of fragments were polymorphic. Observed heterozygosity and F (ST) values were very high in microsatellites, compared with the other markers. Microsatellite and RAPD markers reported a higher degree of genetic differentiation than alloz... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5255720 Thu, 22 Sep 2011 04:00:00 +0100 5255720 http://www.medworm.com/index.php?rid=5237710&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21927814%26dopt%3DAbstract In this study, genetic diversity and population genetic structure of flathead grey mullet, Mugil cephalus, among four China Sea populations were investigated by COI sequences. All the populations studied had high values of haplotype and nucleotide diversity, except for the Yellow Sea population. In the phylogenetic tree, these haplotypes clustered in two groups, one for the populations from the Bohai and East China seas, and the other from the Yellow and South China seas. Analysis of molecular variance indicated that the northern populations (Bohai and East China) had lower genetic divergence (0.0725, P > 0.05) than that of the southern population (South China) (0.4530-0.6827, P < 0.001), suggesting that two distinct genetic groups exist in Chinese waters. Tests of neutral evolut... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5237710 Sat, 17 Sep 2011 04:00:00 +0100 5237710 http://www.medworm.com/index.php?rid=5237709&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21927815%26dopt%3DAbstract We report here for the first time the presence of a novel allele 3, which was absent in all other populations studied to date, in 1.7% each of Singaporean Indians and Malays. We also detected the presence of a novel genotype 3/5 in 3.4% each of Singaporean Indians and Malays. Allele 6, which was absent in Han Chinese from northern China and Taiwan and was also absent in Indians from the Indian subcontinent, was found in 2.1% of Singaporean Chinese and in 0.3% of Singaporean Indians. PMID: 21927815 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5237709 Sat, 17 Sep 2011 04:00:00 +0100 5237709 http://www.medworm.com/index.php?rid=5237711&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21922295%26dopt%3DAbstract Authors: Chen CF, Gan YY Abstract Interleukin-18 (IL-18) plays a key role in autoimmune, inflammatory, and infectious diseases. The IL-18 gene contains a C to A single nucleotide polymorphism (SNP) at position -607 (C-607A) within the promoter region, which was found to affect the promoter activity and subsequently the protein level of IL-18. We investigated this SNP in a group of healthy Singaporeans and found that CA was the most common genotype and the C allele was more prevalent than the A allele, which was not always the case in other ethnic groups. In addition, Singaporean Chinese were significantly different from Singaporean Indians in both allelic and genotypic distributions. Furthermore, significant deviations from Hardy-Weinberg equilibrium of this SNP were found in all t... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5237711 Fri, 16 Sep 2011 04:00:00 +0100 5237711 http://www.medworm.com/index.php?rid=5212979&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21898192%26dopt%3DAbstract This study characterizes the global distribution of type II diabetes-associated TCF7L2 SNPs utilizing HapMap, HGDP-CEPH, and Alfred databases and the literature. High frequencies of rs7903146(T), rs12255372(T), and rs7901695(C) SNPs are observed in Africa, Europe, and the Middle East, but they are reduced and almost absent in Southeast Asian and Native American populations. In contrast, rs11196218(A) has the highest frequency in Eurasia but is reduced in sub-Saharan African and Native American populations. Regional variations in rs7903146(T) follow a gradient of decreasing frequency from southern into northeastern Europe. These findings demonstrate extensive global and regional variations in the frequencies of TCF7L2 SNPs, which may contribute to differences in the incidence of type II dia... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5212979 Wed, 07 Sep 2011 04:00:00 +0100 5212979 http://www.medworm.com/index.php?rid=5212980&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21892776%26dopt%3DAbstract Authors: Chatti N, Zitari-Chatti R, Attia MH, Khadra YB, Said K PMID: 21892776 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5212980 Sun, 04 Sep 2011 04:00:00 +0100 5212980 http://www.medworm.com/index.php?rid=5182954&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21882044%26dopt%3DAbstract In this study, we sequenced the complete coding sequence of the canine NOG gene and characterized the nucleotide polymorphisms. The sequence length varied from 717 to 729 bp, depending on the number of a 6-bp tandem repeat unit (GGCGCG), an insertion that has not been observed in other mammalian NOG genes investigated to date. It results in extensions of (Gly-Ala)(3-5) in the putative NOG protein. To survey the distribution of these tandem repeat polymorphisms, we analyzed 126 individuals in seven dog breeds. We identified only three alleles: (GGCGCG)(3), (GGCGCG)(4), and (GGCGCG)(5). Although the allele frequencies were remarkably different among the breeds, the three alleles were present in all seven of the breeds and did not show any deviation from Hardy-Weinberg equilibrium. PMID:... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5182954 Wed, 31 Aug 2011 23:00:00 +0100 5182954 http://www.medworm.com/index.php?rid=5182956&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21874587%26dopt%3DAbstract Authors: Jiang WY, Zhou BY, Yu GL, Liu H, Zeng JB, Lin QD, Xi HL, Liang H Abstract Knowledge of the G6PD genotype and its associated enzyme activity is significant for population genetics, diagnosis of disease, and management of patients. We tested 2,872 unrelated subjects from a Hakka population in China for G6PD activity by the WHO standard method and for genotype by DHPLC and DNA sequencing. Among female heterozygotes, 78.5% had relatively normal enzyme activity. The phenotype frequency of G6PD deficiency is 0.028, and the causal allele frequency is 0.060 in females. The accuracy, sensitivity, and specificity of DHPLC are more than 98% for detecting G6PD-deficient hemizygotes, heterozygotes, and homozygotes. Measuring enzyme activity alone is not sufficient for the diagnosis of ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5182956 Sat, 27 Aug 2011 23:00:00 +0100 5182956 http://www.medworm.com/index.php?rid=5182955&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21874588%26dopt%3DAbstract This study investigates the role of miRNAs in regeneration. A scrDNA library was constructed, and 17 noncoding RNAs from Eisenia fetida (an optimal model for the study of earthworm regeneration) were cloned and characterized. In addition, reverse transcription polymerase chain reaction was performed to analyze the expression of four small RNAs during different developmental stages. The expression levels of these RNAs in regenerating tissue were higher than in normal tissue, and the expression patterns of these small RNAs were unique during development. PMID: 21874588 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5182955 Sat, 27 Aug 2011 23:00:00 +0100 5182955 http://www.medworm.com/index.php?rid=5170161&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21863358%26dopt%3DAbstract In this study allozyme markers were utilized to assess genetic variations and relationships among 12 distinct populations of this species from the West Himalaya of India. A high level of genetic diversity was found among the populations. Of the 13 loci generated using eight enzymes, 12 (92%) were polymorphic. F-statistics suggested a prevalence of a high heterozygote excess among populations (F (IS) = -0.51). Gene flow estimates and geographic distances between populations did not correlate significantly (r = -0.0258, P < 0.3550). The excess heterozygosity may be attributed to high pollinator mobility and inbreeding coefficient within the subpopulation, relative to the total F (IS) value. High frequencies of several alleles at different loci probably reflect the breeding pattern, ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5170161 Tue, 23 Aug 2011 23:00:00 +0100 5170161 http://www.medworm.com/index.php?rid=5146936&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21858611%26dopt%3DAbstract This study reports on the polymorphism of 15 allozyme loci in Ruditapes decussatus clams collected from 11 locations along the Tunisian coasts. We concentrated our sampling effort around the Siculo-Tunisian region to verify if any population structuring exists in this region and to identify the factors that have shaped this structure. Measurements of genetic diversity were quantified both within and between populations, and the geographic variability of gene frequencies was analyzed. Our study shows that the Siculo-Tunisian Strait is an important genetic boundary between eastern and western regions, which agrees with findings for a variety of other species. We suggest that vicariance is a predominant factor shaping the current distribution of genetic diversity of R. decussatus, and the mix... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5146936 Fri, 19 Aug 2011 23:00:00 +0100 5146936 http://www.medworm.com/index.php?rid=5085386&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21786184%26dopt%3DAbstract Authors: Wan X, Li J, Kim MJ, Park HC, Kim SS, Kim I In an effort to gain greater insight into the nature of the population genetic structure of the pest insect Spodoptera litura (Lepidoptera: Noctuidae), tobacco cutworms were collected from six Korean and five Chinese localities, and their mitochondrial A+T-rich region and nuclear internal transcribed spacer 2 (ITS2) were cloned and sequenced. The A+T-rich region and nuclear ITS2 provided a maximum sequence divergence of 2.88 and 1.82%, respectively. Overall, a low level of genetic fixation (F (ST) = 0-0.02965 in the A+T-rich region and 0-0.34491 in ITS2) and no discernible isolated population were noted among most S. litura populations. Along with these results, the absence of genetic variance between Korea and China indicates a pr... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=5085386 Sat, 23 Jul 2011 23:00:00 +0100 5085386 http://www.medworm.com/index.php?rid=4941104&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21681576%26dopt%3DAbstract Authors: Rajkumar S, Singh SK, Nag A, Ahuja PS Valeriana jatamansi Jones is a natural tetraploid species indigenous to the Indian Himalaya. To assess its genetic diversity and population structure, we analyzed six natural populations from the western Himalayan region using amplified fragment length polymorphism. An analysis of molecular variance found that 93% of the genetic variation of V. jatamansi was within populations and 7% among populations. The correlation between genetic and geographic distances (r = 0.14) was not significant. Though the populations are well separated, the lack of distinct genetic variation between populations may be due to either recent rapid fragmentation from a wide and continuous area resulting in genetically similar populations or wide dispersal of seed... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4941104 Thu, 16 Jun 2011 23:00:00 +0100 4941104 http://www.medworm.com/index.php?rid=4941103&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21681577%26dopt%3DAbstract Authors: Wang ML, Barkley NA, Chen Z, Pittman RN A panel of 55 peanut lines was analyzed for fatty acid composition with gas chromatography and also genotyped with SNP markers from the FAD2 genes by real-time PCR. Significant variation in fatty acid composition was identified, and the ratio of oleic acid to linoleic acid (O/L) ranged from 1.23 to 56.45. In terms of the FAD2 gene mutation, the assayed lines were classified into four genotypes: wild type (Ol(1)Ol(1)Ol(2)Ol(2)), single functional homozygous mutation on the A genome (ol(1)ol(1)Ol(2)Ol(2)), single functional homozygous mutation on the B genome (Ol(1)Ol(1)ol(2)ol(2)), and a double mutation on both A and B genomes (ol(1)ol(1)ol(2)ol(2)). Each genotype has a significantly different fatty acid profile. Both FAD2A and FAD2B are ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4941103 Thu, 16 Jun 2011 23:00:00 +0100 4941103 http://www.medworm.com/index.php?rid=4941102&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21681578%26dopt%3DAbstract Authors: Abdellaoui R, Gouja H, Sayah A, Neffati M Genetic conservation programs in arid environments rely on molecular methods for diversity assessments. DNA-based molecular profiling will aid in conservation and protection of species from genetic erosion. Obtaining intact genomic DNA from Calligonum species, of sufficiently high-quality that is readily amplifiable using PCR, is challenging because of the presence of the exceptionally large amount of oxidized polyphenolic compounds, polysaccharides, and other secondary metabolites. The present method involves a modification of the available CTAB method employing higher concentrations of NaCl and CTAB, and incorporating PEG 6000 (1%) and glucose. The yield of DNA was 60-670 μg g(-1) of fresh tissue. The protocol has been tested with... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4941102 Thu, 16 Jun 2011 23:00:00 +0100 4941102 http://www.medworm.com/index.php?rid=4941105&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21671122%26dopt%3DAbstract Authors: Bouga M, Evangelou V, Lykoudis D, Cakmak I, Hatjina F The scale insect Marchalina hellenica (Gennadius) (Hemiptera: Margarodidae) contributes to the production of pine honey in Turkey and Greece via the honeydew excreted when it feeds on pine trees. Although it is an insect of prime economic importance, there is no information on its genetic structure. Preliminary data were obtained based on sequencing analysis of 12s rDNA and COI mtDNA gene segments from samples from four areas of Turkey. Sequences of the 12s rDNA gene segment from Greek samples available in GenBank were also included. No variability was detected concerning the COI mtDNA gene segment analysis, although 13 haplotypes were revealed based on the 12s rDNA gene segment. The most distant population was from Mudanya... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4941105 Mon, 13 Jun 2011 23:00:00 +0100 4941105 http://www.medworm.com/index.php?rid=4941106&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21660455%26dopt%3DAbstract Authors: Marín S, Gil-Humanes J, Hernando A, Barro F In this work, gliadin composition has been analyzed in 33 accessions of durum wheat using MALDI-TOF MS and compared with A-PAGE results. The MALDI-TOF MS spectra were 29,900-42,500 Da, which corresponds to the α/β- and γ-gliadin regions in A-PAGE. The average of gliadin peaks per line was 23 for MALDI-TOF MS and only 14.8 bands for A-PAGE. MALDI-TOF MS identified 33 gliadin peaks in the durum wheat collection, 20 of which were unique peaks present in 7 lines. A-PAGE analysis identified 30 bands, of which only 4 were unique. Thus, the MALDI-TOF MS method was more sensitive than A-PAGE for identifying α/β- and γ-gliadins in the 33 durum wheat lines studied. Phylogenetic analyses performed using MALDI-TOF MS data assigned the du... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4941106 Thu, 09 Jun 2011 23:00:00 +0100 4941106 http://www.medworm.com/index.php?rid=4941108&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21647824%26dopt%3DAbstract Authors: Li Y, Yang P, Wu S, Yuan J, Shen C, Wu Y, Zhao D, Ren Q, Feng F, Guan W To evaluate the interaction between the angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and gender with individual blood pressure response to hydrochlorothiazide (HCTZ) in hypertensives, we enrolled 829 mild-moderate hypertensive patients. All subjects were given HCTZ (12.5 mg) orally each day for 6 weeks. A total of 776 patients completed the study. There was statistically significant interaction between the effects of genotype and gender on systolic (P = 0.002) and diastolic (P = 0.048) response after adjusting for covariables. Moreover, in each gender, the genotype that was associated with the greatest blood pressure response to HCTZ (DD homozygotes in men and II hom... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4941108 Tue, 07 Jun 2011 23:00:00 +0100 4941108 http://www.medworm.com/index.php?rid=4941107&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21655927%26dopt%3DAbstract Authors: Silva L, Elias RB, Moura M, Meimberg H, Dias E The Azorean endemic gymnosperm Juniperus brevifolia (Seub.) Antoine is a top priority species for conservation in Macaronesia, based on its ecological significance in natural plant communities. To evaluate genetic variability and differentiation among J. brevifolia populations from the Azorean archipelago, we studied 15 ISSR and 15 RAPD markers in 178 individuals from 18 populations. The average number of polymorphic bands per population was 65 for both ISSR and RAPD. The majority of genetic variability was found within populations and among populations within islands, and this partitioning of variability was confirmed by AMOVA. The large majority of population pairwise F (ST) values were above 0.3 and below 0.6. The degree of pop... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4941107 Tue, 07 Jun 2011 23:00:00 +0100 4941107 http://www.medworm.com/index.php?rid=4894010&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21626436%26dopt%3DAbstract Authors: Bao WB, Ye L, Zhu J, Pan ZY, Zhu GQ, Huang XG, Wu SL The alpha (1,2)fucosyltransferase (FUT1) gene has been identified as a candidate gene for controlling the expression of the enterotoxigenic Escherichia coli (ETEC) F18 receptor. Polymorphisms were detected at the M307 position in FUT1 of a breeding base population of Sutai pigs and their correlations to immune parameters analyzed. After digestion by Hin6I, three genotypes were identified at M307, AA (frequency 0.235), AG (0.609), and GG (0.156), with significant deviation from Hardy-Weinberg equilibrium (P < 0.01). The hemoglobin and white blood cell count of the AA genotype pigs were significantly higher than those of AG and GG pigs (P < 0.05). The results indicated that AA pigs not only are resistant to edema dis... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4894010 Mon, 30 May 2011 23:00:00 +0100 4894010 http://www.medworm.com/index.php?rid=4894013&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21607664%26dopt%3DAbstract Authors: Dong C, Zheng X, Li G, Pan C, Zhou M, Hu Z A novel ascorbate peroxidase (APX) cDNA was obtained from Nelumbo nucifera (Elian). The phylogenetic analysis indicated that N. nucifera APX grouped together with chloroplastic APX of high plants. The recombinant protein expressed by PET-30a vector showed APX activity (0.04 mM ascorbate min(-1) mg(-1) protein). The APX mRNA was expressed in young leaves, roots, terminal buds, and leafstalks. Synergistic expression of N. nucifera APX and MnSOD mRNA was indicated in the short-term response to mechanical wounding. PMID: 21607664 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4894013 Mon, 23 May 2011 23:00:00 +0100 4894013 http://www.medworm.com/index.php?rid=4894012&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21607665%26dopt%3DAbstract Authors: Li P, Zha J, Sun H, Song D, Zhou K Expressed sequence tags (ESTs) obtained from two-directional suppression subtractive hybridization (SSH) of cDNA libraries derived from the pleon of the Chinese mitten crab were analyzed using mRNA subtractive hybridization. After cDNA cloning and sequencing, gene expression profiles were examined, focusing on brachyurization. We assembled 211 non-redundant ESTs from SSH library 1 (yielding 130 unique transcripts: 25 consensi, 105 singletons) and 669 from SSH library 2 (195: 51 consensi, 144 singletons). Functional analysis shows that these genes are putatively involved in various cellular processes (33%), ribosomal RNA/proteins (30%), molting (12%), signal transduction (6%), immune factor and stress proteins (6%), development (4%), energy me... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4894012 Mon, 23 May 2011 23:00:00 +0100 4894012 http://www.medworm.com/index.php?rid=4894011&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21607666%26dopt%3DAbstract Authors: Prakash V, Bhattacharya TK, Jyotsana B, Pandey OP PMID: 21607666 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4894011 Mon, 23 May 2011 23:00:00 +0100 4894011 http://www.medworm.com/index.php?rid=4740563&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21509472%26dopt%3DAbstract Authors: Liang YS, Zhang QJ, Wang SQ, Cao LY, Gao ZQ, Li P, Cheng SH Progression to homozygosity of heterozygous genotypes was studied in a cross of the rice subspecies Pei'ai64s and Nipponbare, using a set of 157 polymorphic microsatellite (SSR) markers. The segregation of heterozygous genotypes ranged from 49.13% in the F(2) population to 4.52% in the F(6) population (progression value 11.15%). The heterozygous genotypes were widely distributed in 180 F(2) plants, 330 F(6) lines, and 157 SSR markers. Homozygosity progression showed a wide distribution in plants and SSR markers but not in chromosomes. The segregation of heterozygous genotypes was not significant between populations but varied greatly in F(2) plants, F(6) lines, and SSR markers. The correlation between the progression ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4740563 Wed, 20 Apr 2011 23:00:00 +0100 4740563 http://www.medworm.com/index.php?rid=4740564&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21499900%26dopt%3DAbstract Authors: Gu H, Gong J, Qiu W, Cao H, Xu J, Chen S, Chen Y The nuclear factor of activated T lymphocytes (NFATc1) plays a critical role during valvular and septal development. Genetic variants may influence the biological function of the protein and thus play a role in susceptibility to valvuloseptal defects. Tandem repeat polymorphisms and a common nonsynonymous polymorphism (Cys751Gly) of NFATc1 were genotyped in a hospital-based case-control study of 241 patients with valvuloseptal cardiac defects and 557 controls. The risk of valvuloseptal defect associated with the variant homozygote (LL) was significantly greater than that of the wild-type homozygote. Based on stratification analyses by congenital heart disease types, individuals with the LL genotype were postulated to have a high... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4740564 Sat, 16 Apr 2011 23:00:00 +0100 4740564 http://www.medworm.com/index.php?rid=4740565&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21487739%26dopt%3DAbstract Authors: Zhang Z, Xu BJ, Zhang LB, Sun H, He QG, Wang SW, Sun XW, Liu X PMID: 21487739 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4740565 Tue, 12 Apr 2011 23:00:00 +0100 4740565 http://www.medworm.com/index.php?rid=4740566&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21476017%26dopt%3DAbstract Authors: Ercisli S, Ipek A, Barut E Four well-known commercial olive cultivars (Domat, Edremit, Gemlik, and Memecik) and six local cultivars (Ziraat, Isrange, Tuz, Patos, Yag, and Marantelli) from northeastern Turkey were analyzed for genetic diversity and relationships using seven SSR primers (DCA-4, DCA-09, DCA-11, DCA-16, DCA-17, GAPU-89, UDO-14). The number of markers ranged from 3 (DCA-04 and DCA-17) to 6 (DCA-11, DCA-16, GAPU-89), with an average of 4.57 alleles per primer. UPGMA cluster analysis based on a simple matching similarity matrix grouped cultivars into two main clusters. Three pairs of cultivars (Ziraat and Gemlik, Isrange and Tuz, and Patos and Yag) were thought to be different cultivars although they produced identical SSR profiles. The results indicate the efficienc... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4740566 Thu, 07 Apr 2011 23:00:00 +0100 4740566 http://www.medworm.com/index.php?rid=4682772&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21461907%26dopt%3DAbstract This study describes the organization of the repetitive pattern in the mtDNA control region of Tomistoma schlegelii. Using newly designed primers, we detected length variations of approximately 50-100 bp among individuals, and only one individual showed a heteroplasmic band. Sequencing the region after CSB III revealed two main patterns: a repeat motif and a variable number tandem repeat (VNTR) pattern. The VNTR region, with a core unit of 104 bp, consisting of four motifs and a short AT chain, is implicated in the length variation seen among individuals of Tomistoma. A conserved motif seen in a family unit indicated that the repeat pattern was stably inherited from the maternal parent to all offspring. A combination of VNTR patterns specific to different crocodilians was seen in Tomisto... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4682772 Sat, 02 Apr 2011 23:00:00 +0100 4682772 http://www.medworm.com/index.php?rid=4682773&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21461667%26dopt%3DAbstract This study examined a possible association between the mutations related to Factor V Leiden and Factor II (prothrombin) and stroke in Saudi neonates. A multiplex PCR was established to detect Factor V Leiden G1691A and prothrombin G20210A mutations in 72 neonatal stroke subjects and 70 healthy adult controls with no family history of thromboembolic diseases. The frequency of the homozygous normal genotype (GG) of both genes was found to be significantly lower in the stroke subjects than in the controls (P < 0.0001). The stroke cases also had higher frequencies of the combined Factor II heterozygous mutant form (GA) and the homozygous normal Factor V (GG) (P < 0.0001) and of the combined heterozygous Factor V and the homozygous normal Factor II genotypes (GG) (P = 0.0) than cont... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4682773 Fri, 01 Apr 2011 23:00:00 +0100 4682773 http://www.medworm.com/index.php?rid=4682774&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21448664%26dopt%3DAbstract Authors: Tripathi R, Agarwal S, Ramesh V PMID: 21448664 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4682774 Tue, 29 Mar 2011 23:00:00 +0100 4682774 http://www.medworm.com/index.php?rid=4627169&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21424702%26dopt%3DAbstract Authors: Dos Santos LF, de Oliveira EJ, Dos Santos Silva A, de Carvalho FM, Costa JL, Pádua JG Genetic variation among sweet, purple, and yellow passion fruit accessions was assessed using inter-simple sequence repeat (ISSR) markers. Eighteen ISSR primers were used to evaluate 45 accessions. The number of polymorphic bands per primer varied from 4 to 22, with 12.4 bands per primer on average. Nei's genetic distance between accessions ranged from 0.04 to 0.35. Clustering using the neighbor-joining method resulted in the formation of 11 major clusters. It was not possible to classify the accessions according to their geographic origin, showing that there is no structure in the gene bank. The overall mean Shannon-Weaver diversity index was 0.32, indicating good resolution of genetic dive... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4627169 Tue, 22 Mar 2011 00:00:00 +0100 4627169 http://www.medworm.com/index.php?rid=4627170&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21394610%26dopt%3DAbstract Authors: Altunok V, Yüksek N, Berkman CC, Ağaoğlu ZT, Togan I To determine the genetic structure and variation of Van cats and some other cats, seven enzyme loci were examined using horizontal starch gel electrophoresis. ME bands were observed for the first time in cats. For the enzyme loci CA ( 1 ), SOD, GPI, and GOT, neither the individual Van cats nor the specimens of other cat species exhibited any variation. These enzymes presented identical bands, all of which were homozygous. With respect to the PGD, ME, and ESD loci, however, genetic variation was observed in all of the cats. Hence, three of the seven gene-enzyme systems (43%) were polymorphic with two alleles, contributing to an estimate of average heterozygosity of 0.33-0.49 for the Van cats. PGD was the most discriminator... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4627170 Fri, 11 Mar 2011 00:00:00 +0100 4627170 http://www.medworm.com/index.php?rid=4565134&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21373989%26dopt%3DAbstract Authors: Badaoui B, D'Andrea M, Pilla F, Capote J, Zidi A, Jordana J, Ferrando A, Delgado JV, Martínez A, Vidal O, Amills M Agouti signaling protein (ASIP) is one of the key players in the modulation of hair pigmentation in mammals. Binding to the melanocortin 1 receptor, ASIP induces the synthesis of phaeomelanin, associated with reddish brown, red, tan, and yellow coats. We have sequenced 2.8 kb of the goat ASIP gene in 48 individuals and identified two missense (Cys126Gly and Val128Gly) and two intronic polymorphisms. In silico analysis revealed that the Cys126Gly substitution may cause a structural change by disrupting a highly conserved disulfide bond. We studied its segregation in 12 Spanish and Italian goat breeds (N = 360) with different pigmentation patterns and found stri... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4565134 Sat, 05 Mar 2011 00:00:00 +0100 4565134 http://www.medworm.com/index.php?rid=4565135&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21369822%26dopt%3DAbstract In this study, polymorphism of the Acrp30 gene was detected by PCR-SSCP and DNA sequencing methods in 321 individuals from three goat breeds, and the association of Acrp30 gene polymorphism with growth traits in the three goat breeds was analyzed. A novel insert/deletion (GT)(5) microsatellite sequence was detected in the 5' flanking region of the gene. Three genotypes (AA, AB, and BB) were found in three breeds. There was moderate genetic diversity in the locus in the analyzed populations. Significant associations were observed between the genotypes of the locus and growth traits in the Boer goat population. The chest circumference of individuals with genotype BB was significantly greater than that of individuals with genotype AA. PMID: 21369822 [PubMed - as supplied by publisher] (So... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4565135 Thu, 03 Mar 2011 00:00:00 +0100 4565135 http://www.medworm.com/index.php?rid=4565137&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21360051%26dopt%3DAbstract Authors: Thummajitsakul S, Klinbunga S, Sittipraneed S Genetic diversity and population differentiation of the stingless bee Tetragonula pagdeni (Schwarz) was assessed using single-strand conformational polymorphism (SSCP) analysis of a large subunit of the ribosomal RNA gene (16S rRNA). High levels of genetic variation among individuals within each population (North, Northeast, Central, Prachuap Khiri Khan, Chumphon, and Peninsular Thailand) of T. pagdeni were observed. Analysis of molecular variance indicated significant genetic differentiation among the six geographic populations (Φ (PT) = 0.28, P < 0.001) and between samples collected from north and south of the Isthmus of Kra (Φ (PT) = 0.18, P < 0.001). In addition, Φ (PT) values between all pairwise comparisons w... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4565137 Tue, 01 Mar 2011 00:00:00 +0100 4565137 http://www.medworm.com/index.php?rid=4565136&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21360052%26dopt%3DAbstract Authors: Hu SP, Li H, Zhang FH, Huang LQ, Lu Y We investigated the Y chromosome of various Chinese populations to determine the patrilineal origin of the Chaoshanese population. Admixture analysis of six specific Y short tandem repeat (STR) loci in 6,292 individual samples taken from 51 populations, including Chaoshanese and Minnanese of our earlier studies, showed that over 85% of the Chaoshanese Y chromosomes were derived from the Central China Han (M (RH): 0.8614; M (BE): 1.1868 ± 0.2054), and a very small portion were from the southern aborigines. These results support a Central China Han origin of the Chaoshanese and additionally reveal that males from the Central China Han were the predominant contributor to the patrilineal genetics of the Chaoshanese. A phylogenetic tree and ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4565136 Tue, 01 Mar 2011 00:00:00 +0100 4565136 http://www.medworm.com/index.php?rid=4508892&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21312059%26dopt%3DAbstract Authors: Gopi Chand M, Srinath J, Rao RS, Lakkakula BV, Kumar S, Rao VR Essential hypertension is a complex multifactorial disease caused by interactions between genetic and environmental factors. It is an independent determinant of cardiovascular risk. The main aim of this study was to investigate the possible influence of angiotensinogen M268T polymorphisms on hypertension in two endogamous caste populations of South India. Systolic and diastolic blood pressure, anthropometric variables, and lipid profiles were assessed. Direct sequencing of PCR products was adopted for genotyping. This polymorphism was found to be in Hardy-Weinberg equilibrium in the patients and controls of both populations. Binary odds ratios showed significant association between the M268T polymorphism and hypert... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4508892 Fri, 11 Feb 2011 00:00:00 +0100 4508892 http://www.medworm.com/index.php?rid=4451356&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21279680%26dopt%3DAbstract Authors: Kleinhesselink K, Conway C, Sholer D, Huang I, Kimbrell DA A major category of mutant hematopoietic phenotypes in Drosophila is melanotic tumors or nodules, which consist of abnormal and overproliferated blood cells, similar to granulomas. Our analyses of the melanotic mutant dappled have revealed a novel type of gene involved in blood cell regulation. The dappled gene is an essential gene that encodes cytochrome b5, a conserved hemoprotein that participates in electron transfer in multiple biochemical reactions and pathways. Viable mutations of dappled cause melanotic nodules and hemocyte misregulation during both hematopoietic waves of development. The sexes are similarly affected, but hemocyte number is different in females and males of both mutants and wild type. Additiona... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4451356 Sun, 30 Jan 2011 00:00:00 +0100 4451356 http://www.medworm.com/index.php?rid=4451358&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21274613%26dopt%3DAbstract Authors: Matsunami M, Yoshioka T, Minoura T, Okano Y, Muto Y Human PRTB encodes a proline-rich protein of 168 amino acids (PRTB). We analyzed the evolutionary patterns of PRTB from various vertebrate species. Maximum likelihood analyses indicated that while mammalian PRTB has been very well conserved and underwent a significantly slower rate of evolution, only the branch leading to fish PRTB has undergone adaptive evolution. We generated several mutant PRTBs fused to the GFP variant, Venus, and found that the degradation of PRTB was enhanced by the transfection of an E2, UbcH5. Since mutation of the K153 site in PRTB was refractory to its degradation, proteolysis was suggested to be mediated by ubiquitination of K153. The subcellular localization of PRTB was also investigated, which sh... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4451358 Fri, 28 Jan 2011 00:00:00 +0100 4451358 http://www.medworm.com/index.php?rid=4451357&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21274614%26dopt%3DAbstract Authors: Gauniyal M, Aggarwal A, Kshatriya GK The Siddis are a tribal group of African origin living in Karnataka, India. They have undergone considerable cultural change due to their proximity to neighboring population groups. To understand the biological consequences of these changes, we describe the genomic structure of the Siddis and the contribution from putative ancestral populations using 20 autosomal DNA markers. The distribution of Alu indel markers and a genetic distance analysis reveals their closer affinities with Africans. The levels of genomic diversity and heterozygosity are high in all the populations of southern India. Genetic admixture analysis reveals a predominant contribution from Africans, a lesser contribution from south Indians, and a slight one from Europeans. ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4451357 Fri, 28 Jan 2011 00:00:00 +0100 4451357 http://www.medworm.com/index.php?rid=4451359&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21267645%26dopt%3DAbstract Authors: Yu D, Peng J, Hu S, Wang L, Xin C, Zhang Y, Hu H PMID: 21267645 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4451359 Wed, 26 Jan 2011 00:00:00 +0100 4451359 http://www.medworm.com/index.php?rid=4451361&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21264504%26dopt%3DAbstract This study illustrates the genetic diversity and relationships within and among six Indian sheep breeds of the northwestern arid region of Rajasthan, based on microsatellite markers. The range of allele diversity was 7.72-9.56, and gene diversity was 0.686-0.766, revealing that these breeds possessed substantial amounts of genetic diversity. Positive F (IS) values suggested a deficit of heterozygotes in all six breeds. Despite the declining status of the Marwari, Chokla, Jaisalmeri, Magra and Pugal breeds, an absence of a recent genetic bottleneck was evident from the data. The genetic differentiation estimate (F (ST) = 6.1%) suggested low levels of differentiation between the breeds. Genetic distance estimates revealed a close relationship between the Magra-Pugal and Nali-Jaisalmeri bre... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4451361 Tue, 25 Jan 2011 00:00:00 +0100 4451361 http://www.medworm.com/index.php?rid=4451360&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21264505%26dopt%3DAbstract Authors: Xiong F, Jiang J, Han Z, Zhong R, He L, Zhuang W, Tang R A novel method is introduced for producing molecular markers in plants using single 15- to 18-mer PCR primers designed from the short conserved consensus branch point signal sequences and standard agarose gel electrophoresis. This method was tested on cultivated peanut and verified to give good fingerprinting results in other plant species (mango, banana, and longan). These single primers, designed from relatively conserved branch point signal sequences within gene introns, should be universal across other plant species. The method is rapid, simple, and efficient, and it requires no sequence information of the plant genome of interest. It could be used in conjunction with, or as a substitute for, conventional RAPD or ISS... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4451360 Tue, 25 Jan 2011 00:00:00 +0100 4451360 http://www.medworm.com/index.php?rid=4451362&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21259045%26dopt%3DAbstract Authors: Tao F, Fan M, Zhao W, Lin Q, Ma R A novel ribonuclease (RNase) A superfamily gene (Rdronc) has been cloned from the frog Rana dybowskii. The deduced amino acid sequence shows that it belongs to the ribonuclease A superfamily, with the highest identity, 73%, to Rana pipiens onconase. Adaptive evolution analysis based on maximum likelihood models of codon substitution has been conducted on 10 members of the Rana RNases of subcluster B. Rapid adaptive evolution and multiple positive selection sites have been detected, which indicates that these genes may be evolving under positive selection pressure. Functional assay demonstrates that the recombinant Rdronc protein possesses antimicrobial activity against Gram-negative Escherichia coli and Pseudomonas aeruginosa and weaker antimi... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4451362 Sun, 23 Jan 2011 00:00:00 +0100 4451362 http://www.medworm.com/index.php?rid=4451363&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21258965%26dopt%3DAbstract Authors: Sirivarasai J, Kaojarern S, Sura T, Yoovathaworn K PMID: 21258965 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4451363 Sat, 22 Jan 2011 00:00:00 +0100 4451363 http://www.medworm.com/index.php?rid=4387068&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21253823%26dopt%3DAbstract Authors: Pandith AA, Khan NP, Shah ZA, Shah AM, Wani SM, Siddiqi MA NQO1 gene polymorphism at nucleotide 609 (Pro187Ser) results in a lowering of NQO1 detoxifying activity and is associated with susceptibility to various cancers. The NQO1 genotypes were identified by RFLP in 104 bladder cancer cases and 120 control subjects in an ethnic Kashmiri population. The frequency of the variant NQO1 alleles (CT/TT) was 23.3% for controls and 32.2% for cases (P < 0.05). Overall, the variant alleles were associated with a higher risk of bladder cancer in cases than in the control group (OR = 1.90; 95% CI 1.17-3.04; P < 0.01). In addition, the variant allele genotypes (CT/TT) were associated with a risk of bladder cancer that was more than threefold higher in smokers (OR = 3.47; 95% ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387068 Fri, 21 Jan 2011 00:00:00 +0100 4387068 http://www.medworm.com/index.php?rid=4387067&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21253824%26dopt%3DAbstract Authors: Yang J, Wang Y, Guo ML PMID: 21253824 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387067 Fri, 21 Jan 2011 00:00:00 +0100 4387067 http://www.medworm.com/index.php?rid=4387066&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21253825%26dopt%3DAbstract The objectives of the present study were to identify polymorphisms in the prolactin promoter and estimate their effects on growth traits in White Leghorn chickens. Among 28 haplotypes found, the h1 haplotype was predominant. Body weight at 16 and 64 weeks and age at sexual maturity were significantly associated with haplotype combinations (P < 0.05). The h1/h1 haplogroup showed the highest body weight at 16 weeks of age, and h1/h7 was the highest at 64 weeks. The lowest age at sexual maturity was found in birds with the h1/h6 haplotype combination, and mRNA expression of prolactin was lowest in h1/h4 birds and highest in h1/h5 birds. The prolactin promoter was highly polymorphic and had significant associations with growth traits in White Leghorn chickens. PMID: 21253825 [PubMe... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387066 Fri, 21 Jan 2011 00:00:00 +0100 4387066 http://www.medworm.com/index.php?rid=4387069&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21234669%26dopt%3DAbstract This study presents a new algorithm, SIMLD, to simulate genome populations with real LD structures. SIMLD begins from an initial population with possibly the highest LD level, and then the LD decays to fit the desired level through processes of mating and recombination over generations. SIMLD can produce case-control samples according to various disease models. Using empirical SNP marker information from three populations of HapMap data, we implement the proposed algorithm and demonstrate a set of experimental results. PMID: 21234669 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387069 Fri, 14 Jan 2011 00:00:00 +0100 4387069 http://www.medworm.com/index.php?rid=4387070&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21229300%26dopt%3DAbstract Authors: Akrami H, Soheili ZS, Sadeghizadeh M, Khalooghi K, Ahmadieh H, Kanavi MR, Samiei S, Pakravesh J The retinal pigment epithelium (RPE) plays a key role in the maintenance of the normal functions of the retina. Tissue engineering using amniotic membrane as a substrate to culture RPE cells may provide a promising new strategy to replace damaged RPE. We established a method of culturing RPE cells over the amniotic membrane as a support for their growth and transplantation. The transcription of specific genes involved in cellular function of native RPE, including RPE65, CRALBP, VEGF, CD68, and tyrosinase, were then measured using quantitative real-time PCR. Data showed a considerable increase in transcription of RPE65, CD68, and VEGF in RPE cells cultured on amniotic membrane. The a... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387070 Thu, 13 Jan 2011 00:00:00 +0100 4387070 http://www.medworm.com/index.php?rid=4387073&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21221759%26dopt%3DAbstract Authors: Bani M, Kaabi H, Sellami MH, Chaabane M, Cherif G, Bellali H, Midouni B, Chalfouh N, Dridi A, Jeridi A, Hmida S PMID: 21221759 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387073 Tue, 11 Jan 2011 00:00:00 +0100 4387073 http://www.medworm.com/index.php?rid=4387072&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21221760%26dopt%3DAbstract Authors: Czarnik U, Strychalski J, Zabolewicz T, Pareek CS The allele, genotype, and haplotype frequencies among 837 Polish Holstein-Friesian cattle were determined at two regulatory indel polymorphisms of the PRNP gene. Allele frequencies at the 23 bp indel promoter polymorphism were 0.622 (del) and 0.378 (ins), with 0.613 and 0.387 in sires and 0.633 and 0.366 in dams. Allele frequencies at the 12 bp indel intron polymorphism were 0.527 (del) and 0.473 (ins), with 0.529 and 0.471 in sires and 0.543 and 0.456 in dams. Four haplotypes were identified in this population (23-12del, 23-12ins, 23del-12ins, and 23ins-12del). Haplotype 23-12del occurred most frequently in both sire and dam groups. Comparative analysis of Polish Holstein-Friesian and German Holstein populations revealed a s... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387072 Tue, 11 Jan 2011 00:00:00 +0100 4387072 http://www.medworm.com/index.php?rid=4387071&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21221761%26dopt%3DAbstract Authors: Sala J, Mangolin CA, Franzoni J, de Fátima Pires da Silva Machado M The genetic structure of Cereus peruvianus populations descended from cultivated plants (F(1) populations) and from plants regenerated in vitro (R(1) populations) was analyzed using α- and β-esterase isozymes in native PAGE. The estimated proportion of polymorphic loci was higher (50%) in the R(1) populations than the F(1) populations (42.85%). The mean observed (0.5599) and expected (0.5620) heterozygosity in R(1) descendents was also higher than the rates in F(1) descendents (H (o) = 0.4142; H (e) = 0.4977). A low level of population differentiation was detected in R(1) descendents (F (st) = 0.05). In contrast, population differentiation was high in F(1) descendents (0.2583). Esterase analysis using... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387071 Tue, 11 Jan 2011 00:00:00 +0100 4387071 http://www.medworm.com/index.php?rid=4387076&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21188497%26dopt%3DAbstract Authors: Hong KW, Shin YB, Jin HS, Lim JE, Choi JY, Chang KT, Kim HS, Oh B Recently, genome-wide association studies have identified a strong association between the ZBTB38 locus and human height. In a functional study, we detected two RT-PCR products of ZBTB38, amplified with primers in exons 7 and 8 from a chondrocyte cell line, C-28/I2. Sequencing revealed that the longer product contained an Alu segment in intron 7 of ZBTB38, which contained a potential splicing acceptor site that likely was used to generate the alternative transcript. Insertion of the Alu segment changed the consensus Kozak sequence of the ZBTB38 transcript, potentially altering translational efficiency. We performed RT-PCR using 16 tissue samples from humans and 8 tissue samples from primates to determine any tis... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387076 Wed, 29 Dec 2010 00:00:00 +0100 4387076 http://www.medworm.com/index.php?rid=4387075&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21188498%26dopt%3DAbstract In this study involving subjects from eastern Turkey, the frequencies of HLA-B35, HLA-CW4, HLA-DQ2, and HLA-DQ8 were markedly higher in the chronic HBV group than those in the spontaneously recovered group; the frequencies of HLA-A11 and HLA-A24 in the nonresponsive HBV vaccine group were markedly higher than those in the responsive HBV vaccine group; and the frequency of HLA-CW6 in the nonresponsive HBV vaccine group was significantly lower than in the responsive group. A complete understanding of HLA types associated with the progression to chronic HBV infection and their effects within the cell at the molecular level will be an important contribution in the development of new HBV vaccines and new treatment strategies for chronic HBV infection. PMID: 21188498 [PubMed - as supplied by... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387075 Wed, 29 Dec 2010 00:00:00 +0100 4387075 http://www.medworm.com/index.php?rid=4387074&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21188499%26dopt%3DAbstract This study isolated and characterized 11 novel microsatellite markers for the commercially important swimming crab species, Portunus trituberculatus. Genetic diversity and population structure of two populations of P. trituberculatus in the East China Sea were assessed using these loci. The microsatellite markers produced 242 alleles, varying from 17 to 26 alleles per locus. In all the samples, the range of heterozygosity was 0.6324-0.9403 (observed) and 0.8998-0.9547 (expected). An F-statistic analysis revealed low genetic differentiation between the populations (mean F (ST) = 0.0197), with 98% of the variation resulting from the within-population component. In addition, cross-amplification was tested in two other portunid species, and we found that many loci yielded useful information.... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387074 Wed, 29 Dec 2010 00:00:00 +0100 4387074 http://www.medworm.com/index.php?rid=4387077&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21188496%26dopt%3DAbstract Authors: Scarel-Caminaga RM, Kim YJ, Viana AC, Curtis KM, Corbi SC, Sogumo PM, Orrico SR, Cirelli JA Interleukin-8 (IL-8), which is responsible for the migration and activation of neutrophils, is an important inflammatory mediator involved in the initiation and amplification of acute inflammatory reactions and chronic inflammatory processes. IL-8 plays an important role in periodontitis, an inflammatory disease characterized by the loss of connective tissue and alveolar bone. The aim of this study was to investigate whether the SNPs rs2227307 (+396) and rs2227306 (+781), and the haplotypes they formed together with the previously investigated rs4073 (-251), were associated with chronic periodontitis susceptibility. Clinical periodontal exams were performed and DNA samples were collecte... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4387077 Sat, 25 Dec 2010 00:00:00 +0100 4387077 http://www.medworm.com/index.php?rid=4294046&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21170579%26dopt%3DAbstract Authors: Bagis H, Aktoprakligil D, Gunes C, Arat S, Akkoc T, Cetinkaya G, Kankavi O, Taskin AC, Arslan K, Dundar M, Tsoncheva VL, Ivanov IG PMID: 21170579 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4294046 Sat, 18 Dec 2010 00:00:00 +0100 4294046 http://www.medworm.com/index.php?rid=4294049&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21165766%26dopt%3DAbstract This study provides useful information for further research using zebra finch as a model system. PMID: 21165766 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4294049 Fri, 17 Dec 2010 00:00:00 +0100 4294049 http://www.medworm.com/index.php?rid=4294048&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21165767%26dopt%3DAbstract Authors: Wu Q, Zhan X, Dou T, Chen H, Fan W, Zhou K, Zhang H, Zheng H, Cai Y, Zhao Y, Huang F, Zhou L, Mao Y, Lu D Cytotoxic T lymphocyte-associated antigen-4 (CTLA4) A49G is a polymorphism that is extensively studied in various cancers. To investigate whether it is associated with the occurrence of glioma in Chinese patients, we performed a case-control research study with 670 patients and 680 controls. In this group, we found that the genotype at this locus is significantly associated with glioma risk (GG vs. AA: P = 0.045; GG + AG vs. AA: P = 0.013). In some subgroups, G allele carriers are significantly less represented. We also observed significant correlations between the polymorphism genotype and glioma risk in patients with WHO histologic stages. We conclude that CTLA4 A4... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4294048 Fri, 17 Dec 2010 00:00:00 +0100 4294048 http://www.medworm.com/index.php?rid=4294047&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21165768%26dopt%3DAbstract Authors: Lü A, Hu X, Chen H, Dong Y, Zhang Y, Wang X The single nucleotide polymorphisms (SNPs) within exon 10 of the prolactin receptor gene (PRLR) were detected in Chinese Holstein cows using polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods, and their genetic effects on milk production traits were evaluated in this study. Two newly detected SNPs (g.9206G→A and g.9681C→T) caused amino acid variations E378K and A536V, respectively, which were then preliminarily predicted at the topological level. Statistical results indicated that the two SNPs were significantly associated with milk yields, and cows with the combined genotype GGCC showed superior milk performance. A putative phosphorylation site was identified at residue 378K ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4294047 Fri, 17 Dec 2010 00:00:00 +0100 4294047 http://www.medworm.com/index.php?rid=4294051&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21161366%26dopt%3DAbstract Authors: Oklü R, Hesketh R, Wicky S, Metcalfe JC Latent transforming growth factor-β binding protein-1 (LTBP1) has been implicated in the control of secretion, localization, and activation of TGFβ (transforming growth factor-β). We developed a quantitative reverse-transcriptase polymerase chain reaction (Q-RT-PCR) assay using an RNA internal standard to examine the expression of three alternatively spliced isoforms of LTBP1 (LTBP1Δ41, LTBP1Δ53, and LTBP1Δ55) in a variety of human tissues. The assays were also used to determine the expression of LTBP1L and LTBP1S isoforms and total LTBP1. The Q-RT-PCR assays were highly reproducible and showed that in most tissues LTBP1Δ55 and LTBP1L were minor components of LTBP1. The proportion of LTBP1Δ41 ranged from 2% of total LTBP1 mRNA i... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4294051 Wed, 15 Dec 2010 00:00:00 +0100 4294051 http://www.medworm.com/index.php?rid=4294050&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21161367%26dopt%3DAbstract Authors: Xia X, Zhao J, Du Q, Chang Z To investigate the function and evolutionary origin of the SOXE subgroup, we amplified the genomic DNA of Misgurnus anguillicaudatus using a pair of degenerate primers. Using RACE, we obtained two versions of Sox8 (MaSox8a and MaSox8b) from M. anguillicaudatus. The overall sequence identity of the deduced amino acids from the two genes was 54.38%, with only one amino acid change in the high-mobility group domain. Southern blotting and evidence from the phylogenetic tree provided further proof for the existence of two Sox8 genes at the genomic level. This is the first evidence of two distinct Sox8 genes in Cypriniformes. Semi-quantitative and real-time quantitative PCR assays showed the expression trend of the genes was opposite in early embryonic d... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4294050 Wed, 15 Dec 2010 00:00:00 +0100 4294050 http://www.medworm.com/index.php?rid=4242365&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21132562%26dopt%3DAbstract Authors: Koh HS, Yang BK, Heo SW, Jang KH, In ST To examine the taxonomic status of the Korean red-backed vole (Myodes regulus), the full cytochrome b sequences of 21 red-backed voles from Korea and northeast China were compared with the corresponding haplotypes from 12 species of Myodes and Eothenomys from GenBank. We identified five red-backed voles from Mount Changbai and Harbin as Myodes rufocanus and three from Harbin as M. rutilus, and we confirmed that the red-backed voles from Korea are M. regulus and not Eothenomys regulus. We found that M. regulus from Korea differed from the other five species of Myodes and that the interspecific distances between M. regulus and each of the two species from northeast China were 4.55% (M. rufocanus) and 11.1% (M. rutilus). We concluded that M... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4242365 Sun, 05 Dec 2010 00:00:00 +0100 4242365 http://www.medworm.com/index.php?rid=4242367&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21127960%26dopt%3DAbstract Authors: Chen B, Nie S, Yue Z, Shou W, Xiao C This haplotype-based case-control study investigated whether the aldosterone synthase gene (CYP11B2) might be implicated in the pathogenesis of essential hypertension in Yi (226 individuals) and Hani (296 individuals) minorities of China. Four tag SNPs (rs4536, rs4545, rs3097, and rs3802230) and the K173R polymorphism were genotyped using the PCR-RFLP method. In the Hani minority, rs4536 was significantly associated with hypertension, after Bonferroni correction. H9 AGGC constructed by tag SNPs was significantly higher in hypertensives than in controls (P = 0.001). Further, we observed that haplotype AGGC remained significantly associated with male hypertension after adjustment for covariates (OR = 3.76, P = 0.002). In the Yi minority... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4242367 Fri, 03 Dec 2010 00:00:00 +0100 4242367 http://www.medworm.com/index.php?rid=4242366&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21127961%26dopt%3DAbstract Authors: Urzúa B, Ortega-Pinto A, Morales-Bozo I, Rojas-Alcayaga G, Cifuentes V Amelogenesis imperfecta is a group of genetic conditions that affect the structure and clinical appearance of tooth enamel. The types (hypoplastic, hypocalcified, and hypomature) are correlated with defects in different stages of the process of enamel synthesis. Autosomal dominant, recessive, and X-linked types have been previously described. These disorders are considered clinically and genetically heterogeneous in etiology, involving a variety of genes, such as AMELX, ENAM, DLX3, FAM83H, MMP-20, KLK4, and WDR72. The mutations identified within these causal genes explain less than half of all cases of amelogenesis imperfecta. Most of the candidate and causal genes currently identified encode proteins invo... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4242366 Fri, 03 Dec 2010 00:00:00 +0100 4242366 http://www.medworm.com/index.php?rid=4211444&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21110081%26dopt%3DAbstract Authors: Arias A, Freire R, De La Roche JP, Román G, Méndez J, Insua A Five polymorphic microsatellite loci were identified in the black scallop Mimachlamys varia after construction of a genomic library enriched for (GT)n. To examine the transmission pattern of microsatellite alleles, several families were created and genotypes scored for three loci. The expected Mendelian ratios were found in 12 of 14 segregations examined. Unexpected segregations may be explained by a genotyping error (allelic dropout), given that when a specific allele was treated as dominant, the phenotypic ratios conformed to Mendelian expectations. The five loci were also examined in two samples from the Spanish coast. The two localities displayed similar mean values for the number of alleles per locus (7.2-8.4... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4211444 Fri, 26 Nov 2010 00:00:00 +0100 4211444 http://www.medworm.com/index.php?rid=4163563&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21069449%26dopt%3DAbstract Authors: Gururajan P, Gurumurthy P, Victor D, Srinivasa Nageswara Rao G, Sai Babu R, Sarasa Bharati A, Cherian KM In an analysis of the possible association of endothelial constitutive nitric oxide synthase (ecNOS) gene polymorphism and plasma nitric oxide levels in patients with acute coronary syndrome, we investigated 106 patients with the syndrome and 100 healthy controls. Genotype was determined using the polymerase chain reaction; plasma nitric oxide levels were found using ELISA. The genotype frequencies for the a/b polymorphism in the control group were 77% for bb, 19% for ab, and 4% for aa. In the patients, genotype frequencies were 55% bb, 34% ab, and 11% aa. The allele frequencies were 28% a and 72% b among the patients and 13% a and 87% b among control subjects. Our findings... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4163563 Thu, 11 Nov 2010 00:00:00 +0100 4163563 http://www.medworm.com/index.php?rid=4120841&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20978836%26dopt%3DAbstract Authors: Koundal V, Haq QM, Praveen S The genome of Cucumber mosaic virus New Delhi strain (CMV-ND) from India, obtained from tomato, was completely sequenced and compared with full genome sequences of 14 known CMV strains from subgroups I and II, for their genetic diversity. Sequence analysis suggests CMV-ND shares maximum sequence identity at the nucleotide level with a CMV strain from Taiwan. Among all 15 strains of CMV, the encoded protein 2b is least conserved, whereas the coat protein (CP) is most conserved. Sequence identity values and phylogram results indicate that CMV-ND belongs to subgroup I. Based on the recombination detection program result, it appears that CMV is prone to recombination, and different RNA components of CMV-ND have evolved differently. Recombinational anal... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4120841 Wed, 27 Oct 2010 00:00:00 +0100 4120841 http://www.medworm.com/index.php?rid=4120842&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20976539%26dopt%3DAbstract Authors: Mukherjee AK, Ratha S, Dhar S, Debata AK, Acharya PK, Mandal S, Panda PC, Mahapatra AK Genetic relationships among 22 taxa of bamboo were evaluated using 12 inter simple sequence repeats (ISSR) and four expressed sequence tag (EST)-based random primers, resulting in amplification of 220 loci. The grouping of species based on Jaccard's similarity matrix using UPGMA and principal coordinate analysis agreed with earlier published reports on molecular phylogenetic studies in bamboos with few deviations. In the dendrogram, however, species of one genus were placed in different clusters along with members of other genera. This calls for correct taxonomic delineation at the genus and species level using both vegetative and reproductive characters and correlation of molecular data wit... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4120842 Tue, 26 Oct 2010 00:00:00 +0100 4120842 http://www.medworm.com/index.php?rid=4106482&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20972704%26dopt%3DAbstract Authors: Muneer PM, Sivanandan R, Gopalakrishnan A, Basheer VS, Musammilu KK, Ponniah AG Random-amplified polymorphic DNA (RAPD) and microsatellite markers were developed and used for the analysis of genetic variability in the critically endangered yellow catfish Horabagrus nigricollaris, sampled from the Chalakkudy River, Kerala, India. Eight RAPD and five microsatellite markers were detected to genotype the species. In RAPD, the 73 fragments were 20.55% polymorphic, whereas 4 polymorphic loci (80%) were obtained in microsatellites. In microsatellites, the number of alleles across the 5 loci was 1-5, and the range of heterozygosity was 0.25-0.5. The mean observed number of alleles was 2.4, and the effective number was 1.775 per locus. The average heterozygosity across all investigated... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4106482 Fri, 22 Oct 2010 23:00:00 +0100 4106482 http://www.medworm.com/index.php?rid=4106483&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20963631%26dopt%3DAbstract Authors: Arslan S, Bardakci F This present study investigated micro- and macro-geographic microsatellite DNA variations using five polymorphic microsatellite loci from 27 brown trout populations in Turkey. Average number of alleles and average observed heterozygosity were 7.4 and 0.254, respectively. Even populations from the same sea basin and river system (the so called micro-geographic regions) had unique alleles. Genetic variation among the populations from macro-geographic regions (different sea basins and river systems) was 45.78%. The mtDNA lineages of brown trout that have previously been identified by mtDNA analyses were supported by the analysis of the microsatellite DNA data in general. The Çatak population, which belongs to the Tigris lineage, was clustered together with t... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4106483 Wed, 20 Oct 2010 23:00:00 +0100 4106483 http://www.medworm.com/index.php?rid=4056889&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20936502%26dopt%3DAbstract This study investigated SULT1A1 and SULT1A2 gene polymorphism using a PCR-RFLP method (n = 303). The frequency of the SULT1A1*1 allele was 76.2% and SULT1A1*2 was 23.8%. The SULT1A1*3 allele could not be identified. The SULT1A2 frequencies were 69.2% (SULT1A2*1), 18.3% (SULT1A2*2), and 12.5% (SULT1A2*3). The SULT1A1 and SULT1A2 loci were in Hardy-Weinberg equilibrium (SULT1A1 χ(2) = 0.58, P = 0.44; SULT1A2 χ(2) = 7.28, P = 0.06). Linkage analysis indicated a close linkage between these two genes (χ(2) = 5.31, P < 0.01); therefore, the statistical hypothesis that SULT1A1 and SULT1A2 alleles are independently distributed was rejected. Additionally, a strongly positive linkage was detected between SULT1A1*2 and SULT1A2*2 alleles in this population (D' = 0.79, χ(2) = 3... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4056889 Sat, 09 Oct 2010 23:00:00 +0100 4056889 http://www.medworm.com/index.php?rid=4056891&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20931357%26dopt%3DAbstract Authors: Kidd LR, Hein DW, Woodson K, Taylor PR, Albanes D, Virtamo J, Tangrea JA Genetic variations in xenobiotic metabolizing genes can influence susceptibility to many environmentally induced cancers. Inheritance of the N-acetyltransferase 1 allele (NAT1*10), linked with increased metabolic activation of pro-carcinogens, is associated with an increased susceptibility to many cancers in which cigarette- or meat-derived carcinogens have been implicated in their etiology. The role of NAT1*10 in prostate cancer is under studied. Although cigarette smoking is not considered a risk factor for prostate cancer, a recent review suggests it may play a role in disease progression. Consequently, we examined the association of NAT1*10 with prostate cancer risk, grade, and stage among 400 Finnish... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4056891 Thu, 07 Oct 2010 23:00:00 +0100 4056891 http://www.medworm.com/index.php?rid=4056890&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20931358%26dopt%3DAbstract Authors: Zhang G, Ding F, Wang J, Dai G, Xie K, Zhang L, Wang W, Zhou S In our research, single nucleotide polymorphisms (SNPs) of exon regions of the myostatin gene were detected by PCR-SSCP in the Bian chicken and three reference chicken populations (Jinghai, Youxi, and Arbor Acre). Four novel SNPs (G2283A, C7552T, C7638T, and T7661A) were detected. The findings from the least square means showed that Bian chickens with EE and DE genotypes had significantly higher body weight, at 6-18 weeks of age, than those of the DD genotype (P < 0.05). The results suggest that the mutation G2283A, detected in exon 1, has potential as a genetic marker for body weight traits in the Bian chicken. PMID: 20931358 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4056890 Thu, 07 Oct 2010 23:00:00 +0100 4056890 http://www.medworm.com/index.php?rid=4037239&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20924784%26dopt%3DAbstract Authors: Kang JF, Li XL, Zheng HQ, Zhou RY, Li LH, Zhao HY Much attention has been focused on the study of lactoferrin at the protein or nucleotide level in mice, humans, and cattle, but little is known about it in goats. The goat LF gene from 5' UTR to exon 17 was amplified, and the variation of g.7605C→T in 10 Chinese indigenous goat breeds was analyzed. Among the three ruminant species (cattle, sheep, and goats), the intron-exon distribution pattern was similar, and all the exons had the same length, but the length of introns varied greatly due to insertions or deletions. The frequency of allele T at g.7605C→T (50.12%) was a little higher than that of allele C (49.88%), and the genotype distribution differed greatly between goat populations. The g.7605C→T site showed higher ge... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4037239 Tue, 05 Oct 2010 23:00:00 +0100 4037239 http://www.medworm.com/index.php?rid=4037238&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20924785%26dopt%3DAbstract Authors: Pugliesi C, Fambrini M, Salvini M ent-Kaurene, a key precursor of gibberellins, is formed by the action of two diterpene synthases (diTPSs), ent-copalyl diphosphate synthase (CPS), and ent-kaurene synthase (KS). The full-length cDNAs of CPS- (HaCPS1L) and KS-like (HaKS2L and HaKS3L) genes were isolated from sunflower. The amino acid sequences of HaCPS1L, HaKS2L, and HaKS3L exhibit structural features and homology to diTPSs of several plant species involved in gibberellin biosynthesis. RT-PCR analysis indicates that the expression of all genes (HaCPS1L, HaKS2L, and HaKS3L) is highly regulated during growth and development. All three diTPSs are preferentially expressed in rapidly growing tissues. HaKS2L is expressed at a much lower level than the other two diTPS genes. During se... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4037238 Tue, 05 Oct 2010 23:00:00 +0100 4037238 http://www.medworm.com/index.php?rid=4023854&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20878545%26dopt%3DAbstract This study assessed the distribution pattern of allelic variants at the prolactin-RsaI locus in 23 Indian native cattle breeds (Bos indicus). PCR-RFLP genotyping of a 156 bp fragment of prolactin (PRL) in exon 3 revealed the predominance of the heterozygous AB genotype (mean frequency 0.58) irrespective of utility type (dairy, dual, draft), geographic region (northern, central, southern), and coat color (red, gray) of the breeds analyzed. The overall frequencies of homozygous AA (0.22) and BB (0.20) genotypes were in a similar range. The PRL (A) and PRL (B) alleles exhibited similar gene frequencies (means 0.52 and 0.48, respectively). The existing profile of the PRL-RsaI gene locus in a large set of Indian native cattle breeds was different from that of Bos taurus and cattle breeds of ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4023854 Tue, 28 Sep 2010 23:00:00 +0100 4023854 http://www.medworm.com/index.php?rid=4023855&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20862607%26dopt%3DAbstract Authors: Ohsaka Y, Yogosawa S, Nakanishi R, Sakai T, Nishino H Gene promoter regions of p15(INK4B), a cyclin-dependent kinase inhibitor, and phosphatase and tensin homolog (PTEN), a dual-function protein and lipid phosphatase, interact with regulatory factors for gene transcription and methylation. Normal individuals exhibit sequence polymorphisms in these regulatory genes. We isolated genomic DNA from whole blood of healthy Japanese individuals and sequenced promoter regions of the p15 ( INK4B ) and PTEN genes. We also examined the influence of polymorphisms on promoter activity in several cell lines. We identified polymorphisms at positions -699, -394, and -242 and an insertion at position -320 in the p15 ( INK4B ) gene and a polymorphism at position -1142 in the PTEN gene. Reporter ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4023855 Wed, 22 Sep 2010 23:00:00 +0100 4023855 http://www.medworm.com/index.php?rid=4023856&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20848177%26dopt%3DAbstract Authors: Zhu J, Zhong LQ, Zhang CF, Liu H, Li B PMID: 20848177 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=4023856 Thu, 16 Sep 2010 23:00:00 +0100 4023856 http://www.medworm.com/index.php?rid=3969708&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20839046%26dopt%3DAbstract Authors: Zhou R, Li X, Zheng H, Li L, Quan W, Shen Z, Tong J, Yang Q, Fu Q Scrapie is a prion disease in sheep and goats. Ribosomal protein SA (RPSA), also called 37 kDa laminin receptor precursor/67 kDa laminin receptor has been demonstrated to be a putative cell surface receptor for prion. To investigate the caprine RPSA, we cloned the full-length coding sequence of the gene of goat and submitted it to GenBank. The length of the open reading frame is 888 bp, encoding 295 amino acids. The putative amino acid sequence is highly similar to that of other mammals. The caprine amino acid sequence of RPSA is shown to be identical to the sequence of species susceptible to scrapie at positions 241, 272, and 291. The phylogenetic tree analysis revealed that the genetic distance betwee... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3969708 Mon, 13 Sep 2010 23:00:00 +0100 3969708 http://www.medworm.com/index.php?rid=3960636&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20820904%26dopt%3DAbstract This study thus helps to outline a genotype-phenotype relation for the PON1 gene in a Turkish population. PMID: 20820904 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3960636 Mon, 06 Sep 2010 23:00:00 +0100 3960636 http://www.medworm.com/index.php?rid=3960637&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20820903%26dopt%3DAbstract Authors: Abbasi H, Tahmoorespour M, Nassiri MR, Ghovvati S RAPD markers were used to investigate population genetic parameters of an endangered partridge, Alectoris chukar, in four areas of Iran, as a part of a genetic conservation program. The aim of this study was to analyze the genetic similarity among these populations. Blood samples from 75 birds were used for DNA extraction and RAPD-PCR analysis of 67 loci, with 28 polymorphic bands (41.79%). The populations of Kalat-e-Nader and Mashhad were found to be closely related, as were the Torbat-e-Jaam and the Quchan populations. Mean heterozygosity for all populations was 0.4405 +/- 0.0755. The results indicate that chukar partridge genetic diversity in Khorasan-e-Razavi province is sufficient and the amount of gene flow among populati... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3960637 Sat, 04 Sep 2010 23:00:00 +0100 3960637 http://www.medworm.com/index.php?rid=3960635&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20820905%26dopt%3DAbstract Authors: Huang Y, Wang Y, He D, Liu Y Melanocortin 4 receptor (MC4R) plays a crucial part in regulating feeding behavior in humans and rodents. We detected two single nucleotide polymorphisms (SNPs; c.108G --> A and c.627C --> T) in the goose MC4R gene and genotyped 94 Landes geese for association analysis with several carcass traits. Significant associations (P < 0.05) were obtained for c.108G --> A with carcass weight, breast muscle percentage, and leg muscle percentage, and for c.627C --> T with body weight, carcass weight, semi-eviscerated weight, and eviscerated weight. We re-constructed haplotypes based on the two SNPs and analyzed diplotypes in association with carcass traits, obtaining significant associations with several of the traits. These results suggest tha... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3960635 Sat, 04 Sep 2010 23:00:00 +0100 3960635 http://www.medworm.com/index.php?rid=3960634&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20820906%26dopt%3DAbstract Authors: Wang CM, Way TD, Chang YC, Yen NT, Hu CL, Nien PC, Jea YS, Chen LR, Kao JY In order to avoid interference from nuclear copies of mitochondrial DNA (numts), mtDNA of the white Roman goose (domestic goose) was extracted from liver mitochondria. The mtDNA control region was amplified using a long PCR strategy and then sequenced. Neighbor-joining, maximum parsimony, and maximum-likelihood approaches were implemented using the 1,177 bp mtDNA control region sequences to compute the phylogenetic relationships of the domestic goose with other geese. The resulting identity values for the white Roman geese were 99.1% (1,166/1,177) with western graylag geese and 98.8% (1,163/1,177) with eastern graylag geese. In molecular phylogenetic trees, the white Roman goose was grouped in the grayl... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3960634 Sat, 04 Sep 2010 23:00:00 +0100 3960634 http://www.medworm.com/index.php?rid=3960638&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20811773%26dopt%3DAbstract We report a Tunisian family of three sisters with moderate mental retardation, facial dysmorphism, cardiopathy, and bilateral clinodactyly of the third and fourth toes, explored by MLPA, showing the same associated microduplications, 15q and Xq, without a concurrent deletion. PMID: 20811773 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3960638 Wed, 01 Sep 2010 23:00:00 +0100 3960638 http://www.medworm.com/index.php?rid=3960639&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20809087%26dopt%3DAbstract Authors: Biramijamal F, Basatvat S, Hossein-Nezhad A, Soltani MS, Akbari Noghabi K, Irvanloo G, Shamimi K PMID: 20809087 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3960639 Mon, 30 Aug 2010 23:00:00 +0100 3960639 http://www.medworm.com/index.php?rid=3914525&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20798985%26dopt%3DAbstract Authors: Wang M, Sheng Z, Wu X, Wang Q, Pan Y There are extensive debates concerning the origin and the necessity of conservation of Pudong White pigs in the Taihu region of China. To elucidate their genetic ancestry, we sequenced the complete mtDNA D-loop sequences of 25 Pudong White pigs and 9 Shawutou pigs from the Taihu region and reanalyzed 158 published sequences of other breeds in GenBank. Haplotypes of all pigs were classified into two clades, A and E, but Pudong White pigs were found only in clade A. The results of population differentiation demonstrated that there were few genetic exchanges among the eight populations. Furthermore, divergence time estimates suggested that the Pudong White pigs split from four types of Taihu pigs at about 0.26-0.75 million years ago. Overall, ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3914525 Thu, 26 Aug 2010 23:00:00 +0100 3914525 http://www.medworm.com/index.php?rid=3817850&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20677020%26dopt%3DAbstract Authors: Wagner S, McCracken J, Cole S, Laible G Site-specific gene modifications in cells are initiated by the introduction of exogenous DNA. We used a recently established cell assay to compare the ability of DNA donors to induce a single point mutation that converts a target gene encoding blue fluorescent protein (BFP) into expressing green fluorescent protein (GFP). In a chromosomal assay with cells stably expressing BFP, we showed that fluorescently labeled single-stranded oligonucleotides and a donor plasmid cotranscribing a red fluorescent protein provide similar efficiencies in triggering BFP-GFP conversions. In transient cotransfections, an isogenic donor plasmid comprising a nonfunctional GFP gene yielded a greater efficiency for the conversion of the BFP target gene than a n... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3817850 Fri, 30 Jul 2010 23:00:00 +0100 3817850 http://www.medworm.com/index.php?rid=3808455&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20668929%26dopt%3DAbstract Authors: Siqueira MV, Pinheiro TT, Borges A, Valle TL, Zatarim M, Veasey EA Using nine microsatellite loci, we investigated genetic structure and diversity in 83 Brazilian cassava accessions, including several landraces, in the Cerrado biome in Mato Grosso do Sul, Brazil. All nine loci were polymorphic, averaging 6.00 alleles per locus. Treating each of seven municipalities as a cassava group or population, they averaged 3.5 alleles per locus, with 97% polymorphic loci, high values for observed heterozygosity (0.32) and gene diversity (0.56). Total genetic variability was high (0.668), and most of this genetic variability was concentrated within municipalities (0.577). Cluster and structure analyses divided accessions into two major clusters or populations (K = 2). Also, a significant ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3808455 Wed, 28 Jul 2010 23:00:00 +0100 3808455 http://www.medworm.com/index.php?rid=3808456&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20658315%26dopt%3DAbstract This study analyzed polymorphism of the caprine GHSR gene as a genetic marker candidate for growth traits in goats. Two single nucleotide polymorphisms (GU014697:g.165G-->A and GU014697:g.548T-->C) were identified in exon 2 of the caprine GHSR gene by PCR-single-strand conformation polymorphism and DNA sequencing methods. Their associations with growth traits were analyzed in 313 Xuhuai goats. The results indicated that GU014697:g.548T-->C had significant effects on growth traits. Body length and body length index were significantly higher in individuals with genotype TT than CC and CT in (P < 0.05). TT individuals also tended to have better performance in other traits, such as body height and chest circumference, although there were no statistical differences (P > 0.05). Th... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3808456 Sat, 24 Jul 2010 23:00:00 +0100 3808456 http://www.medworm.com/index.php?rid=3784795&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20652395%26dopt%3DAbstract Authors: Boussaid M, Benito C, Harche MK, Naranjo T, Zedek M Intermicrosatellite PCR [inter-simple sequence repeat (ISSR)-PCR] markers and cytogenetics criteria were used to assess the level of genetic diversity and genetic structure in 17 populations of Stipa tenacissima (Gramineae) from Algeria. All populations sampled in the steppe area were diploids (2n = 2x = 24), and those sampled in the dry area were hexaploids (2n = 6x = 72). The dendrogram based on ISSR-PCR showed homogeneity within populations and large variability among populations. All individuals of the same population were gathered and formed groups clearly separated in all populations. These groups were separated into two clusters related to biotope, one from the steppe area and the other from the dry area. AMOVA indicat... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3784795 Tue, 20 Jul 2010 23:00:00 +0100 3784795 http://www.medworm.com/index.php?rid=3773351&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20640884%26dopt%3DAbstract Authors: Yilmaz KU, Yanar M, Ercisli S, Sahiner H, Taskin T, Zengin Y The genus Crataegus is well distributed in Turkey as a wild plant, with numerous, inherently variable species and genotypes. RAPD markers were used to study 17 hawthorn genotypes belonging to Crataegus monogyna ssp. monogyna Jacq (2 genotypes), C. monogyna ssp. azarella Jacq (1), Crataegus pontica K.Koch (3), Crataegus orientalis var. orientalis Pallas Ex Bieb (3), Crataegus pseudoheterophylla Pojark (1), Crataegus aronia var. dentata Browicz (1), C. aronia var. aronia Browicz (4), and Crateagus x bornmuelleri Zabel (2). The 10 RAPD primers produced 72 polymorphic bands (88% polymorphism). A dendrogram based on Jaccard's index included four major groups and one outgroup according to taxa. The lowest genetic variabili... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3773351 Sat, 17 Jul 2010 23:00:00 +0100 3773351 http://www.medworm.com/index.php?rid=3758284&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20628805%26dopt%3DAbstract Authors: He G, He B, Racey PA, Cui J Type I interferons (IFNs) are produced by leukocytes in reaction to pathogenic infection and function as positive mediators in antiviral pathways. Among IFNs, IFN alpha (IFNA) has the largest number of family members and plays an important role against the invasion of pathogens. Bats are putative and proven vectors for numerous viruses; however, the evolution of the IFNA family in bats has not been addressed. Here, we construct a phylogeny of IFNA families, including one fruit bat (Dobsonia viridis), with other vertebrates as references. Site-model estimation reveals that positive selection has shaped bat IFNA genes, showing that positive selection drives the evolution of bat IFNA genes. PMID: 20628805 [PubMed - as supplied by publisher] (Source... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3758284 Tue, 13 Jul 2010 23:00:00 +0100 3758284 http://www.medworm.com/index.php?rid=3758283&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20628806%26dopt%3DAbstract Authors: Zhang CL, Gao XY, Shao RY, Wang YH, Fang XT, Chen H There is evidence that stearoyl-CoA desaturase (SCD) is a key enzyme for lipid metabolism. Changes in enzyme activity that depends on SCD gene polymorphism and regulation could cause variations of fatty acid composition of meat and milk. We investigated genetic variability in caprine SCD, analyzing 335 animals belonging to three goat breeds by single-strand conformation polymorphism and DNA sequencing. Six single nucleotide polymorphism were identified, in intron 3 (585T-->A and 601A-->G; Ref. AF422168), intron 4 (719T-->A; AF422169), and exon 6 (690 A-->G, 718 C-->G, and 802 A-->C; AF422171). The less polymorphic SNP 601A-->G displayed variability only in Xuhuai and Boer breeds. Across breeds, the averag... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3758283 Tue, 13 Jul 2010 23:00:00 +0100 3758283 http://www.medworm.com/index.php?rid=3758282&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20628807%26dopt%3DAbstract Authors: Satoh N, Watanabe N, Kanda A, Sugaya-Fukasawa M, Hisatomi H PMID: 20628807 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3758282 Tue, 13 Jul 2010 23:00:00 +0100 3758282 http://www.medworm.com/index.php?rid=3758281&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20628808%26dopt%3DAbstract Authors: Sinha E, Walia GK, Gupta BP, Ghosh PK, Saraswathy KN AvaII and NcoI polymorphisms in the low-density lipoprotein receptor (LDL-R) gene are reported to alter cholesterol levels. Although found to be highly polymorphic worldwide, these mutations have not been validated in any Indian population. This case-control association study was conducted in an endogamous business community of Delhi. Blood samples from 100 cases and 100 age- and sex-matched controls belonging to the same ethnic group were subjected to biochemical and molecular analyses. Medical history and anthropometric measurements were taken from all the enrolled subjects. Linkage disequilibrium between the two polymorphisms was found to be significant (P = 0.0016). Significant variability was observed for the AvaII poly... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3758281 Tue, 13 Jul 2010 23:00:00 +0100 3758281 http://www.medworm.com/index.php?rid=3758280&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20628809%26dopt%3DAbstract In this study, RAPD (60), ISSR (48), RAMPO (63), and SSR (34) markers were compared to detect polymorphism and to establish genetic relationships among Tunisian fig tree cultivars. The statistical procedures conducted on the combined data show considerable genetic diversity, and the tested markers discriminated all fig genotypes studied. The identification key established on the basis of SSR permitted the unambiguous discrimination of cultivars and confirmed the reliability of SSR for fingerprinting fig genotypes. The study findings are discussed in relation to the establishment of a national reference collection that will aid in the conservation of Tunisian fig resources. PMID: 20628809 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3758280 Tue, 13 Jul 2010 23:00:00 +0100 3758280 http://www.medworm.com/index.php?rid=3758285&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20623331%26dopt%3DAbstract Authors: Shimogiri T, Msalya G, Myint SL, Okamoto S, Kawabe K, Tanaka K, Mannen H, Minezawa M, Namikawa T, Amano T, Yamamoto Y, Maeda Y Six polymorphic sites of the bovine prion protein gene (PRNP) were genotyped in 569 animals of Asian native cattle, Japanese breeds, purebred mythun (Bos frontalis), and mythun x cattle composite animals. At the 23-bp indel site, a deletion (23-) allele was a major allele in all populations except mythun. At the 12-bp indel site, an insertion (12+) allele was a major allele in all populations. The 14-bp indel site was polymorphic in all Asian native cattle. In the octapeptide repeat region, a six-repeat allele was a major allele in all populations, and 5/5 and 4/6 genotypes were detected in Japanese Black and Mongolian cattle and in mythun, respectivel... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3758285 Sat, 10 Jul 2010 23:00:00 +0100 3758285 http://www.medworm.com/index.php?rid=3741211&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20607386%26dopt%3DAbstract In this study, based on PCR-SSCP and DNA sequencing methods, polymorphisms of the PC1 gene were detected in 447 individuals from three breeds. Only the P(1), P(2), P(3), P(9), and P(10) loci showed polymorphisms, and 12 SNPs in the PC1 gene were identified. Additionally, an association analysis between mutations in the 5'-UTR and four growth traits indicated that the polymorphisms were significantly associated with caprine body height (P < 0.05) and chest circumference (P < 0.01). PMID: 20607386 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3741211 Tue, 06 Jul 2010 23:00:00 +0100 3741211 http://www.medworm.com/index.php?rid=3688111&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20549331%26dopt%3DAbstract Authors: Singh RK, Lal KK, Mohindra V, Punia P, Sah RS, Mishra AK, Kumar R, Mishra BN, Lakra WS The population structure of Labeo calbasu from 11 rivers belonging to the Indus, Ganges, Bhima, Mahanadi, and Godavari basins was investigated using allozyme marker systems. Seven out of 20 allozyme loci (35%) were polymorphic (P < 0.99). Both probability and score tests indicated significant deviation of genotype proportions from Hardy-Weinberg expectations at two loci, XDH* (Mahanadi, Bhima, and Godavari) and G6PDH* (Mahanadi). A pairwise genetic homogeneity test and F (ST) values indicated a low-to-moderate level (0.0515) of genetic structuring in the wild population of L. calbasu. AMOVA analysis also indicated moderate differentiation among the samples from different river basins. Ana... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3688111 Sat, 12 Jun 2010 23:00:00 +0100 3688111 http://www.medworm.com/index.php?rid=3688110&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20549332%26dopt%3DAbstract The objective of this study was to identify genetic polymorphisms of the CACNA2D1 gene and to analyze associations between SNPs and carcass and meat quality traits in cattle. Through PCR-RFLP and DNA sequencing methods, a new allelic variant corresponding to the A --> G mutation (aspartic to glycine amino acid replacement) of the bovine CACNA2D1 gene was detected. Two alleles and three genotypes (AA, AG, and GG) were defined. Genetic character indicated that the A526745G locus showed moderate polymorphism and was in Hardy-Weinberg equilibrium. Gene-specific SNP marker association analysis showed that the A526745G mutant was significantly associated with carcass weight, dressing percentage, meat percentage, and backfat thickness. The results add new evidence that CACNA2D1 is an important... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3688110 Sat, 12 Jun 2010 23:00:00 +0100 3688110 http://www.medworm.com/index.php?rid=3688109&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20549333%26dopt%3DAbstract This study will improve understanding of the relationship between structural and functional diversity of SUMO paralogs during vertebrate evolution. PMID: 20549333 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3688109 Sat, 12 Jun 2010 23:00:00 +0100 3688109 http://www.medworm.com/index.php?rid=3688112&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20544268%26dopt%3DAbstract Authors: Chatterjee RN, Bhattacharya TK, Dange M, Rajkumar U To measure genetic relatedness between populations, for breeding purposes, we analyzed 170 birds from six crossbred populations of three pure lines of White Leghorn chickens, using 14 microsatellite markers. All the microsatellites were polymorphic, with 2-6 alleles. The mean number of alleles per locus was 3.21. The effective number of alleles varied from 1.14 to 3.94. The observed heterozygosity varied from 0.133 to 1.00, with a mean of 0.748. The F (IS) values were mostly negative, with an average of -0.345. The mean F (ST) value was 0.056. The Nm values ranged from 1.91 to 42.17. The highest genetic identity was observed between IWI x IWK and IWK x IWI. The relation between any two groups of crosses was more than 85%. The... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3688112 Thu, 10 Jun 2010 23:00:00 +0100 3688112 http://www.medworm.com/index.php?rid=3641624&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20526734%26dopt%3DAbstract Authors: Solorzano CD, Szalanski AL, Owens CB, Dayton Steelman C The floodwater mosquito Aedes vexans, a potential vector of West Nile virus, has a worldwide distribution that includes the continental United States and southern Canada. In order to determine the effect that Hurricane Katrina had on the temporal genetic variation of Ae. vexans from New Orleans, we compared genetic diversity of a portion of the mtDNA ND5 gene of mosquito specimens collected during 2005 (n = 99) with specimens from 2006 (n = 103), after the hurricane. Average haplotype diversity (Hd) was high (>0.88) in 2005 and 2006 for both the parishes studied. It does not appear that Hurricane Katrina had any impact on genetic diversity, and despite the intense efforts to control mosquitoes in New Orleans, Ae. vexan... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3641624 Fri, 04 Jun 2010 23:00:00 +0100 3641624 http://www.medworm.com/index.php?rid=3641623&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20526735%26dopt%3DAbstract Authors: Koh HS, Jang KH PMID: 20526735 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3641623 Fri, 04 Jun 2010 23:00:00 +0100 3641623 http://www.medworm.com/index.php?rid=3641622&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20526736%26dopt%3DAbstract Authors: Koh HS, Zhang M, Bayarlkhagva D, Ham EJ, Kim JS, Jang KH, Park NJ We reexamined Tamias sibiricus barberi from Korea by sequencing c-myc exon 2 and the mtDNA control region. In the c-myc exon, the monogenic T. s. barberi differed from the monogenic T. s. orientalis (nucleotide distance 0.48%; 3 variable sites at 168, 306, and 552), whereas T. s. orientalis was identical to T. s. sibiricus. In the control region, T. s. barberi differed from T. s. orientalis (distance 6.84%) and T. s. sibiricus (9.35%). We considered the concordant, extensive gaps between the phylogroup of T. s. barberi and other subspecies of T. sibiricus in the c-myc gene, control region, and cytochrome b gene to be evidence of a lack of intergradation through North Korea from T. s. barberi to T. s. orientalis.... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3641622 Fri, 04 Jun 2010 23:00:00 +0100 3641622 http://www.medworm.com/index.php?rid=3632836&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20524055%26dopt%3DAbstract Authors: Jia Z, Chi X, Li C, Shi L The major histocompatibility complex (MHC) has an important role in immune response and is known as the most polymorphic locus in vertebrates. We developed three pairs of polymerase chain reaction primers of the alpha-2 domain (exon 3) of MHC class I and the beta-2 (exon 3) and beta-3 domains (exon 4) of MHC class II B gene in the German mirror common carp (Cyprinus carpio L.). We analyzed the three loci in a population of 65 individuals that had suffered the serious disease of gill rot. Five to six variable nucleotide sites and two to six variable amino acid sites (71.43%) were detected in the exon sequence of the sampled populations, indicating that many of them corresponded to amino acids involved in antigen recognition. Deviation from Hardy-Weinbe... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3632836 Thu, 03 Jun 2010 23:00:00 +0100 3632836 http://www.medworm.com/index.php?rid=3632835&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20524056%26dopt%3DAbstract Authors: Xu TJ, Quan XQ, Sun YN, Zhao KC, Wang RX PMID: 20524056 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3632835 Thu, 03 Jun 2010 23:00:00 +0100 3632835 http://www.medworm.com/index.php?rid=3553147&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20454948%26dopt%3DAbstract This study investigated a possible role of the RET proto-oncogene in sporadic HSCR patients in the Han Chinese population. Our results indicated that rs1800858, rs1800860, rs1800863, and rs2075912, located in exons 2, 7, 15, and intron 19 of RET, are strongly associated with the disease (P < 0.01), with rs1800860 and rs1800863 playing a protective role in the pathogenesis of HSCR in the Chinese population. We also showed that the haplotype consisting of four SNPs is significantly associated with HSCR. We did not find a significant difference in the CA-repeat in intron 5 of RET between cases and controls. Our study provided further evidence that the RET gene is involved in the susceptibility to HSCR in the Han Chinese population. PMID: 20454948 [PubMed - in process] (Source: Biochemi... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3553147 Wed, 12 May 2010 08:24:08 +0100 3553147 http://www.medworm.com/index.php?rid=3553146&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20454949%26dopt%3DAbstract In this study, an Atlantic halibut (Hippoglossus hippoglossus) homolog of the p8 gene was cloned, sequenced, and characterized. The full-length p8 cDNA consists of 601 bp and encodes 76 amino acids with a molecular mass of 9 kD. The bHLH region is well conserved between Atlantic halibut and other animals. Analysis by RT-PCR showed that the p8 transcript is constitutively expressed in 9 of the 12 tissues tested: pancreas, intestine, stomach, gill, head kidney, heart, liver, ovary, and spleen. A predicted microRNA target site was found in the 3'UTR of Atlantic halibut p8 mRNA. We speculate that the target site may pair to microRNA molecules because the target site resides in a big loop, a space large enough for the binding of microRNA molecules. PMID: 20454949 [PubMed - in process] (Sour... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3553146 Wed, 12 May 2010 08:24:05 +0100 3553146 http://www.medworm.com/index.php?rid=3490884&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20405317%26dopt%3DAbstract Authors: Wang MB, Hao ZZ Thirteen natural populations from throughout the range of the Chinese pine (Pinus tabulaeformis Carr.) were examined using inter-simple sequence repeat markers to characterize the genetic structure at the species level and to compare the extent and distribution of genetic variation among central, intermediate, and marginal populations. Although the total genetic variation in the Chinese pine was mainly maintained within populations, the genetic differentiation among populations was significant (P < 0.001). The genetic divergence was significantly correlated with geographic distance (P < 0.05). Genetic diversity tended to decrease from the central to intermediate and marginal populations. The marginal populations had significantly lower intrapopulation gen... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3490884 Mon, 19 Apr 2010 23:00:00 +0100 3490884 http://www.medworm.com/index.php?rid=3472392&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20390337%26dopt%3DAbstract Authors: Sharma RK, Negi MS, Sharma S, Bhardwaj P, Kumar R, Bhattachrya E, Tripathi SB, Vijayan D, Baruah AR, Das SC, Bera B, Rajkumar R, Thomas J, Sud RK, Muraleedharan N, Hazarika M, Lakshmikumaran M, Raina SN, Ahuja PS India has a large repository of important tea accessions and, therefore, plays a major role in improving production and quality of tea across the world. Using seven AFLP primer combinations, we analyzed 123 commercially important tea accessions representing major populations in India. The overall genetic similarity recorded was 51%. No significant differences were recorded in average genetic similarity among tea populations cultivated in various geographic regions (northwest 0.60, northeast and south both 0.59). UPGMA cluster analysis grouped the tea accessions accord... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3472392 Tue, 13 Apr 2010 23:00:00 +0100 3472392 http://www.medworm.com/index.php?rid=3472391&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20390338%26dopt%3DAbstract This study examined whether adipocyte volume is under the influence of genetic factors and evaluated its genetic correlations with AST. Fasting plasma levels of 344 pedigreed baboons from the Southwest National Primate Research Center in San Antonio, TX, USA, were assayed for AST. Adipocyte volume was measured using biopsies of omental adipose tissue. Adipocyte volume, body weight, and plasma AST were heritable. Genetic correlations between the measured adiposity-related phenotypes and AST were significant. A quantitative trait locus (LOD score 3.2) for adipocyte volume was identified on the baboon homolog of human chromosome 6 near marker D6S1028. These results suggest that omental adipocyte volume is under genetic regulation and that shared genetic factors influence adiposity-associated ... Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3472391 Tue, 13 Apr 2010 23:00:00 +0100 3472391 http://www.medworm.com/index.php?rid=3472390&cid=s_37583_50_f&fid=37583&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20390339%26dopt%3DAbstract This study is the first to characterize eggplant F. oxysporum species using ISSR and RAPD. PMID: 20390339 [PubMed - as supplied by publisher] (Source: Biochemical Genetics) Biochemical Genetics journals http://www.medworm.com/rss/comments.php?id=3472390 Tue, 13 Apr 2010 23:00:00 +0100 3472390