MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Biochimica et Biophysica Acta' source. Thu, 09 Feb 2012 13:38:27 +0100 http://www.medworm.com/index.php?rid=5637016&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22265024%26dopt%3DAbstract Authors: Gold BT, Keller JN PMID: 22265024 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5637016 Sat, 28 Jan 2012 21:06:18 +0100 5637016 http://www.medworm.com/index.php?rid=5595518&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22225970%26dopt%3DAbstract Authors: Allen TW, Separovic F PMID: 22225970 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595518 Tue, 17 Jan 2012 00:21:06 +0100 5595518 http://www.medworm.com/index.php?rid=5595485&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240168%26dopt%3DAbstract CONCLUSIONS: Cumulative frontal affinity chromatography (FAC) data demonstrated that the intracellular lectins exhibit distinct sugar-binding specificity profiles. The glycotopes recognized by these lectins as fate determinants are embedded in the triantennary structures of the high-mannose-type oligosaccharides and are exposed upon trimming of the outer glucose and mannose residues during the N-glycan processing pathway. Furthermore, recently emerged 3D structural data offer mechanistic insights into functional interplay between an intracellular lectin and its binding partner in the early secretory pathway. GENERAL SIGNIFICANCE: Structural biology approaches in conjunction with FAC methods provide atomic pictures of the mechanisms behind the glycoprotein-fate determination in cells. This ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595485 Thu, 05 Jan 2012 05:00:00 +0100 5595485 http://www.medworm.com/index.php?rid=5595513&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22236806%26dopt%3DAbstract This article is part of a Special Issue entitled: Respiratory Oxidases. PMID: 22236806 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595513 Wed, 04 Jan 2012 05:00:00 +0100 5595513 http://www.medworm.com/index.php?rid=5595512&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22236836%26dopt%3DAbstract This article is part of a Special Issue entitled: VDAC structure, function, and regulation of mitochondrial metabolism. PMID: 22236836 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595512 Wed, 04 Jan 2012 05:00:00 +0100 5595512 http://www.medworm.com/index.php?rid=5595511&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22236837%26dopt%3DAbstract This article is part of a Special Issue entitled: Protein Folding in Membranes. PMID: 22236837 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595511 Wed, 04 Jan 2012 05:00:00 +0100 5595511 http://www.medworm.com/index.php?rid=5595510&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240008%26dopt%3DAbstract We present here a comparative study of two members of the family, PepFect3 (PF3) and PepFect6 (PF6), together with their parental CPP transportan-10 (TP10), and their interactions with lipid membranes. We show that the addition of a stearyl moiety to TP10 increases the amphipathicity of these molecules and their ability to insert into a lipid monolayer composed of zwitterionic phospholipids. The addition of negatively charged phospholipids into the monolayer results in decreased binding and insertion of the stearylated peptides, indicating modification in the balance of hydrophobic versus electrostatic interactions of peptides with lipid bilayer, thus revealing some clues for the selective interaction of these CPPs with different lipids. The trifluoromethylquinoline moieties, in PF6 make n... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595510 Wed, 04 Jan 2012 05:00:00 +0100 5595510 http://www.medworm.com/index.php?rid=5595509&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240009%26dopt%3DAbstract Authors: Lee J, Glover KJ Abstract Caveolin is an integral membrane protein that is found in high abundance in caveolae. Both the N- and C- termini lie on the same side of the membrane, and the transmembrane domain has been postulated to form an unusual intra-membrane horseshoe configuration. To probe the structure of the transmembrane domain, we have prepared a construct of caveolin-1 that encompasses residues 96-136 (the entire intact transmembrane domain). Caveolin-1(96-136) was over-expressed and isotopically labeled in E. coli, purified to homogeneity, and incorporated into lyso-myristoylphosphatidylglycerol micelles. Circular dichroism and NMR spectroscopy reveal that the transmembrane domain of caveolin-1 is primarily α-helical (57-65%). Furthermore, chemical shift indexing... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595509 Wed, 04 Jan 2012 05:00:00 +0100 5595509 http://www.medworm.com/index.php?rid=5595507&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240167%26dopt%3DAbstract CONCLUSION: The low relative concentration of galectins in BAL makes it likely that functional interactions with glycoproteins occur at sites rich in galectin, such as cells of the airways, rather than the extracellular fluid itself. The profile of galectin bound proteins differed between samples from asthma patients and healthy subjects and correlated with the presence of fibroblast or eosinophils. This included appearance of a specific galectin-8-binding glycoform of haptoglobin, previously shown to be increased in serum in other inflammatory conditions. GENERAL SIGNIFICANCE: It is technically feasible to identify galectin-binding glycoproteins in low concentration patient samples such as BAL-fluid, to generate biomedically interesting results. This article is part of a Special Issue ent... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595507 Wed, 04 Jan 2012 05:00:00 +0100 5595507 http://www.medworm.com/index.php?rid=5595461&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240386%26dopt%3DAbstract This article is part of a Special Issue entitled: Chromatin in time and space. PMID: 22240386 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595461 Wed, 04 Jan 2012 05:00:00 +0100 5595461 http://www.medworm.com/index.php?rid=5595516&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22233557%26dopt%3DAbstract This article is part of a Special Issue entitled: Chromatin in time and space. PMID: 22233557 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595516 Tue, 03 Jan 2012 05:00:00 +0100 5595516 http://www.medworm.com/index.php?rid=5595514&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22234330%26dopt%3DAbstract Authors: Costa TF, Reis FC, Lima AP Abstract The cysteine protease brucipain is an important drug target in the protozoan Trypanosoma brucei, the causative agent of both Human African trypanosomiasis and Animal African trypanosomiasis. Brucipain is closely related to mammalian cathepsin L and currently used as a framework for the development of inhibitors that display anti-parasitic activity. We show that recombinant brucipain lacking the C-terminal extension undergoes inhibition by the substrate benzyloxycarbonyl-FR-7-amino-4-methylcoumarin at concentrations above the K(m), but not by benzyloxycarbonyl-VLR-7-amino-4-methylcoumarin. The allosteric modulation exerted by the substrate is controlled by temperature, being apparent at 25°C but concealed at 37°C. The behavior of the en... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595514 Tue, 03 Jan 2012 05:00:00 +0100 5595514 http://www.medworm.com/index.php?rid=5595508&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240010%26dopt%3DAbstract This article is part of a Special Issue entitled: VDAC structure, function, and regulation of mitochondrial metabolism. PMID: 22240010 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595508 Tue, 03 Jan 2012 05:00:00 +0100 5595508 http://www.medworm.com/index.php?rid=5595464&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240200%26dopt%3DAbstract Authors: Mercher T, Quivoron C, Couronné L, Bastard C, Vainchenker W, Bernard OA Abstract The TET family of proteins has been described a few years ago. Only recently, their roles in DNA modification, through the oxidation of methyl-cytosine, and in normal and malignant development, through the description of TET2 as a tumor suppressor have been documented. The conjunction of these findings has prompted large efforts to understand the biology of these novel entities. Here, we summarize the recent results implicating TET2 in hematological malignancies suggesting that further studies are required to fully understand the role of DNA methylation alterations during transformation. PMID: 22240200 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595464 Tue, 03 Jan 2012 05:00:00 +0100 5595464 http://www.medworm.com/index.php?rid=5595462&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240201%26dopt%3DAbstract Authors: Kim J, Yu J Abstract Major breakthroughs at the beginning of this century in high-throughput technologies have profoundly transformed biological research. Significant knowledge has been gained regarding our biological system and its disease such as malignant transformation. In this review, we summarize leading discoveries in prostate cancer research derived from the use of high-throughput approaches powered by microarrays and massively parallel next-generation sequencing (NGS). These include the seminal discovery of chromosomal translocations such as TMPRSS2-ERG gene fusions as well as the identification of critical oncogenes exemplified by the polycomb group protein EZH2. We then demonstrate the power of interrogating genomic and epigenomic data in understanding the pleth... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595462 Tue, 03 Jan 2012 05:00:00 +0100 5595462 http://www.medworm.com/index.php?rid=5595460&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22240387%26dopt%3DAbstract This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing. PMID: 22240387 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595460 Tue, 03 Jan 2012 05:00:00 +0100 5595460 http://www.medworm.com/index.php?rid=5595517&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22230192%26dopt%3DAbstract In conclusion, 8-Br-cAMP stimulated α-MG uptake via Epac and PKA-dependent SGLTs expression and trafficking through cav-1 and F-actin in PTCs. PMID: 22230192 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595517 Mon, 02 Jan 2012 05:00:00 +0100 5595517 http://www.medworm.com/index.php?rid=5595515&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22234285%26dopt%3DAbstract Authors: Wang W, Linsdell P Abstract Opening and closing of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel are controlled by ATP binding and hydrolysis by its nucleotide binding domains (NBDs). This is presumed to control opening of a single "gate" within the permeation pathway, however, the location of such a gate has not been described. We used patch clamp recording to monitor access of cytosolic cysteine reactive reagents to cysteines introduced into different transmembrane (TM) regions in a cysteine-less form of CFTR. The rate of modification of Q98C (TM1) and I344C (TM6) by both [2-sulfonatoethyl] methanethiosulfonate (MTSES) and permeant Au(CN)(2)(-) ions was reduced when ATP concentration was reduced from 1mM to 10μM, and modification by MTS... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5595515 Mon, 02 Jan 2012 05:00:00 +0100 5595515 http://www.medworm.com/index.php?rid=5576771&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22208916%26dopt%3DAbstract Authors: Erdmann R PMID: 22208916 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5576771 Sun, 01 Jan 2012 05:00:00 +0100 5576771 http://www.medworm.com/index.php?rid=5489304&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22137373%26dopt%3DAbstract Authors: de Jonge WJ, van den Wijngaard RM PMID: 22137373 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5489304 Sat, 10 Dec 2011 20:12:22 +0100 5489304 http://www.medworm.com/index.php?rid=5489303&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22142840%26dopt%3DAbstract Authors: Turk V PMID: 22142840 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5489303 Sat, 10 Dec 2011 20:12:12 +0100 5489303 http://www.medworm.com/index.php?rid=5414194&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22082451%26dopt%3DAbstract Authors: Rich PR PMID: 22082451 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5414194 Thu, 17 Nov 2011 12:54:50 +0100 5414194 http://www.medworm.com/index.php?rid=5414193&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22082452%26dopt%3DAbstract Authors: Mitchell P Abstract 50years ago Peter Mitchell proposed the chemiosmotic hypothesis for which he was awarded the Nobel Prize for Chemistry in 1978. His comprehensive review on chemiosmotic coupling known as the first "Grey Book", has been reprinted here with permission, to offer an electronic record and easy access to this important contribution to the biochemical literature. This remarkable account of Peter Mitchell's ideas originally published in 1966 is a landmark and must-read publication for any scientist in the field of bioenergetics. As far as was possible, the wording and format of the original publication have been retained. Some changes were required for consistency with BBA formats though these do not affect scientific meaning. A scanned version of the original ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5414193 Thu, 17 Nov 2011 12:54:40 +0100 5414193 http://www.medworm.com/index.php?rid=5414192&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22085541%26dopt%3DAbstract Authors: Cenacchi L, Busch M, Schleidt PG, Müller FG, Stumpp TV, Mäntele W, Trost P, Lancaster CR Abstract Cytochrome (cyt) b(561) proteins are dihaem-containing membrane proteins, belonging to the CYBASC (cytochrome-b(561)-ascorbate-reducible) family, and are proposed to be involved in ascorbate recycling and/or the facilitation of iron absorption. Here, we present the heterologous production of two cyt b(561) paralogs from Arabidopsis thaliana (Acytb(561)-A, Acytb(561)-B) in Escherichia coli and Pichia pastoris, their purification, and initial characterisation. Spectra indicated that Acytb(561)-A resembles the best characterised member of the CYBASC family, the cytochrome b(561) from adrenomedullary chromaffin vesicles, and that Acytb(561)-B is atypical compared to other CYBASC... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5414192 Mon, 07 Nov 2011 05:00:00 +0100 5414192 http://www.medworm.com/index.php?rid=5414191&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22085605%26dopt%3DAbstract Authors: Biancini GB, Vanzin CS, Rodrigues DB, Deon M, Ribas G, Barschak AG, Manfredini V, Netto CB, Jardim LB, Giugliani R, Vargas CR Abstract Fabry disease is an X-linked inborn error of glycosphingolipid catabolism due to deficient activity of α-galactosidase A that leads to accumulation of the enzyme substrates, mainly globotriaosylceramide (Gb3), in body fluids and lysosomes of many cell types. Some pathophysiology hypotheses are intimately linked to reactive species production and inflammation, but until this moment there is no in vivo study about it. Hence, the aim of this study was to investigate oxidative stress parameters, pro-inflammatory cytokines and Gb3 levels in Fabry patients under treatment with enzyme replacement therapy (ERT) and finally to establish a possible ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5414191 Sun, 06 Nov 2011 04:00:00 +0100 5414191 http://www.medworm.com/index.php?rid=5414190&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22086149%26dopt%3DAbstract This article is part of a Special Issue entitled: Respiratory oxidases. PMID: 22086149 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5414190 Sun, 06 Nov 2011 04:00:00 +0100 5414190 http://www.medworm.com/index.php?rid=5377278&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F10%252F07%252022.14%2522%255BMHDA%255D%253A%25222011%252F11%252F05%252006.35%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F10%252F07%252022.14%2522%255BEDAT%255D%29%29 302 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/11/05PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5377278 Sat, 05 Nov 2011 10:35:04 +0100 5377278 http://www.medworm.com/index.php?rid=5294287&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21554952%26dopt%3DAbstract Authors: Jian B, Yang S, Chen D, Chaudry I, Raju R Abstract Trauma-hemorrhage (T-H) causes hypoxia and organ dysfunction. Mitochondrial dysfunction is a major factor for cellular injury due to T-H. Aging also has been known to cause progressive mitochondrial dysfunction. In order to study the effect of aging on T-H-induced mitochondrial dysfunction, we recently developed a rodent mitochondrial genechip with probesets representing mitochondrial and nuclear genes contributing to mitochondrial structure and function. Using this chip we recently identified signature mitochondrial genes altered following T-H in 6 and 22month old rats; augmented expression of the transcription factor c-myc was the most pronounced. Based on reports of c-myc-IL6 collaboration and c-myc-Sirt1 negative regul... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294287 Sat, 08 Oct 2011 02:19:18 +0100 5294287 http://www.medworm.com/index.php?rid=5294286&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21558001%26dopt%3DAbstract Authors: Nur E, Verwijs M, de Waart DR, Schnog JJ, Otten HM, Brandjes DP, Biemond BJ, Elferink RP, Abstract Erythrocytes are both an important source and target of reactive oxygen species in sickle cell disease. Levels of glutathione, a major antioxidant, have been shown to be decreased in sickle erythrocytes and the mechanism leading to this deficiency is not known yet. Detoxification of reactive oxygen species involves the oxidation of reduced glutathione (GSH) into glutathione-disulfide (GSSG) which is actively transported out of erythrocyte. We questioned whether under oxidative conditions, GSSG efflux is increased in sickle erythrocytes. Erythrocytes of 18 homozygous sickle cell patients and 9 race-matched healthy controls were treated with 2,3-dimethoxy-l,4-naphthoquinone, w... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294286 Sat, 08 Oct 2011 02:19:07 +0100 5294286 http://www.medworm.com/index.php?rid=5294285&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21683140%26dopt%3DAbstract Authors: Fletcher JL, Kondagari GS, Wright AL, Thomson PC, Williamson P, Taylor RM Abstract The processes regulating the complex neurodegenerative cascade of vacuolation, neuroinflammation, neuronal loss and myelin deficits in fucosidosis, a neurological lysosomal storage disorder, remain unclear. To elucidate these processes the gene expression profile of the cerebral cortex from untreated and intrathecal enzyme replacement therapy treated fucosidosis pups and age-matched unaffected controls were examined. Neuroinflammation and cell death processes were identified to have a major role in fucosidosis pathophysiology with 37% of differentially expressed (DE) genes involved in these processes. Critical, specific, early decreases in expression levels of key genes in myelin assembly we... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294285 Sat, 08 Oct 2011 02:18:56 +0100 5294285 http://www.medworm.com/index.php?rid=5294284&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21749920%26dopt%3DAbstract Authors: Dolinsky VW, Dyck JR Abstract Calorie restriction is one of the most effective nutritional interventions that reproducibly protects against obesity, diabetes and cardiovascular disease. Recent evidence suggests that even when implemented over a short period, calorie restriction is a safe and effective treatment for cardiovascular disease. Herein, we review the effects of calorie restriction on the cardiovascular system as well as the biological effects of resveratrol, the most widely studied molecule that appears to mimic calorie restriction. An overview of microarray data reveals that the myocardial transcriptional effects of calorie restriction overlap with the transcriptional responses to resveratrol treatment. In addition, calorie restriction and resveratrol modulate s... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294284 Sat, 08 Oct 2011 02:18:45 +0100 5294284 http://www.medworm.com/index.php?rid=5294283&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21757002%26dopt%3DAbstract In conclusion, FXR activation regulates glutamine and glutamate metabolism and FXR ligands might have utility in the treatment of hyperammonemia states. PMID: 21757002 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294283 Sat, 08 Oct 2011 02:18:34 +0100 5294283 http://www.medworm.com/index.php?rid=5294282&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21763422%26dopt%3DAbstract CONCLUSIONS: Pro-fibrotic transformation of HSCs through the loss of Sept4 is, in part, due to reduced expression of Dkk2 and its homologues, and the resulting disinhibition of the canonical Wnt pathway. PMID: 21763422 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294282 Sat, 08 Oct 2011 02:18:23 +0100 5294282 http://www.medworm.com/index.php?rid=5294281&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21763423%26dopt%3DAbstract Authors: Samuni Y, Baum BJ Abstract In vivo gene delivery has long been seen as providing opportunities for the development of novel treatments for disorders refractory to existing therapies. Over the last two decades, salivary glands have proven to be a useful, if somewhat unconventional, target tissue for studying several potential clinical applications of therapeutic gene delivery. Herein, we follow the progress, address some problems and assess the outlook for clinical applications of salivary gland gene delivery. Our experience with these tissues provides a roadmap for the process of moving an idea from the laboratory bench to patients. PMID: 21763423 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294281 Sat, 08 Oct 2011 02:18:12 +0100 5294281 http://www.medworm.com/index.php?rid=5294280&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21767639%26dopt%3DAbstract Authors: Remor AP, de Matos FJ, Ghisoni K, da Silva TL, Eidt G, Búrigo M, de Bem AF, Silveira PC, de León A, Sanchez MC, Hohl A, Glaser V, Gonçalves CA, Quincozes-Santos A, Borba Rosa R, Latini A Abstract Large scale clinical trials have demonstrated that an intensive antihyperglycemic treatment in diabetes mellitus (DM) in individuals reduces the incidence of micro- and macrovascular complications, e.g. nephropathy, retinopathy, DM-accelerated atherosclerosis, myocardial infarction, or limb amputations. Here, we investigated the effect of short- and long-term insulin administration on mitochondrial function in peripheral tissues of streptozotocin (STZ)-induced hyperglycemic rats. In addition, the in vitro effect of methylglyoxal (MG), advanced glycation end products (AGEs) and ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294280 Sat, 08 Oct 2011 02:18:01 +0100 5294280 http://www.medworm.com/index.php?rid=5294279&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21784149%26dopt%3DAbstract Authors: Kokjohn TA, Van Vickle GD, Maarouf CL, Kalback WM, Hunter JM, Daugs ID, Luehrs DC, Lopez J, Brune D, Sue LI, Beach TG, Castaño EM, Roher AE Abstract Amyloid-β (Aβ) peptides are intimately involved in the inflammatory pathology of atherosclerotic vascular disease (AVD) and Alzheimer's disease (AD). Although substantial amounts of these peptides are produced in the periphery, their role and significance to vascular disease outside the brain requires further investigation. Amyloid-β peptides present in the walls of human aorta atherosclerotic lesions as well as activated and non-activated human platelets were isolated using sequential size-exclusion columns and HPLC reverse-phase methods. The Aβ peptide isolates were quantified by ELISA and structurally analyzed using MA... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294279 Sat, 08 Oct 2011 02:17:49 +0100 5294279 http://www.medworm.com/index.php?rid=5294278&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21787863%26dopt%3DAbstract Authors: Wong AS, Cheung ZH, Ip NY Abstract Macroautophagy maintains cellular homeostasis through targeting cytoplasmic contents and organelles into autophagosomes for degradation. This process begins with the assembly of protein complexes on isolation membrane to initiate the formation of autophagosome, followed by its nucleation, elongation and maturation. Fusion of autophagosomes with lysosomes then leads to degradation of the cargo. In the past decade, significant advances have been made on the identification of molecular players that are implicated in various stages of macroautophagy. Post-translational modifications of macroautophagy regulators have also been demonstrated to be critical for the selective targeting of cytoplasmic contents into autophagosomes. In addition, rece... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294278 Sat, 08 Oct 2011 02:17:39 +0100 5294278 http://www.medworm.com/index.php?rid=5294277&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21787864%26dopt%3DAbstract Authors: Haller JF, Krawczyk SA, Gostilovitch L, Corkey BE, Zoeller RA Abstract Inherited glucose-6-phosphate isomerase (GPI) deficiency is the second most frequent glycolytic erythroenzymopathy in humans. Patients present with non-spherocytic anemia of variable severity and with neuromuscular dysfunction. We previously described Chinese hamster (CHO) cell lines with mutations in GPI and loss of GPI activity. This resulted in a temperature sensitivity and severe reduction in the synthesis of glycerolipids due to a reduction in phosphatidate phosphatase (PAP). In the current article we attempt to describe the nature of this pleiotropic effect. We cloned and sequenced the CHO lipin 1 cDNA, a gene that codes for PAP activity. Overexpression of lipin 1 in the GPI-deficient cell line, G... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294277 Sat, 08 Oct 2011 02:17:28 +0100 5294277 http://www.medworm.com/index.php?rid=5294276&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21791244%26dopt%3DAbstract Authors: Wang XT, Mion B, Aherne A, Engel PC Abstract Retinitis pigmentosa, causing progressive blindness, is genetically heterogeneous. RP10, due to a defect in inosine monophosphate dehydrogenase 1 (IMPDH1), shows autosomal dominant inheritance. Recombinantly expressed clinical mutants show unaltered kinetic behaviour. It is unclear why reportedly impaired DNA binding is important and how it would explain negative dominance. An alternative view relates to the mutant proteins' tendency to aggregate. Regarding negative dominance, a key question is whether the defective protein can subvert the function of its normal counterpart in the same cell. Potentially, the homotetrameric structure of IMPDH1 might offer a vehicle for such an effect. We have established a reliable protocol for r... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294276 Sat, 08 Oct 2011 02:17:17 +0100 5294276 http://www.medworm.com/index.php?rid=5294275&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21810471%26dopt%3DAbstract Conclusion. Here we report a novel splicing mutation IVS7 +3A>G, identified in a family with mild form LQT1 phenotypes, and examined functional outcome in comparison with three other variants around the exon 7-intron 7 junction. In addition to c.1032G>A mutation, IVS7 +3A>G generates exon-skipping mRNAs, and thereby causing LQT1 phenotype. The severity of clinical phenotypes appeared to differ between the two splicing-related mutations and to result from the amount of resultant mRNAs and their functional consequences. PMID: 21810471 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294275 Sat, 08 Oct 2011 02:17:06 +0100 5294275 http://www.medworm.com/index.php?rid=5294274&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21820508%26dopt%3DAbstract In conclusion, we show that the oligomerization process and conformational stability are altered by mutations that could affect the physiological behavior of the enzyme. This endorses the hypothesis that oligomerization and folding defects of PAH variants are the most common causes of HPAs, particularly as regards mild human phenotypes. PMID: 21820508 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294274 Sat, 08 Oct 2011 02:16:56 +0100 5294274 http://www.medworm.com/index.php?rid=5294273&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21827850%26dopt%3DAbstract Authors: Oussoren E, Brands MM, Ruijter GJ, der Ploeg AT, Reuser AJ Abstract The mucopolysaccharidoses (MPS) are prominent among the lysosomal storage diseases. The intra-lysosomal accumulation of glycosaminoglycans (GAGs) in this group of diseases, which are caused by several different enzyme deficiencies, induces a cascade of responses that affect cellular functions and maintenance of the extra-cellular matrix. Against the background of normal tissue-specific processes, this review summarizes and discusses the histological and biochemical abnormalities reported in the bones, joints, teeth and extracellular matrix of MPS patients and animal models. With an eye to the possibilities and limitations of reversing the pathological changes in the various tissues, we address therapeutic ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294273 Sat, 08 Oct 2011 02:16:45 +0100 5294273 http://www.medworm.com/index.php?rid=5294272&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21839169%26dopt%3DAbstract In conclusion, the interaction of p-radixin with Mrp2 was decreased by the activation of PKC and PP-1 under oxidative stress conditions which subsequently led to Mrp2 internalization, whereas the interaction of p-radixin and Mrp2 was increased by the activation of PKA during recovery from oxidative stress. PMID: 21839169 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294272 Sat, 08 Oct 2011 02:16:33 +0100 5294272 http://www.medworm.com/index.php?rid=5294271&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21839170%26dopt%3DAbstract Authors: Wang W, Guo Y, Xu M, Huang HH, Novikova L, Larade K, Jiang ZG, Thayer TC, Frontera JR, Aires D, Ding H, Turk J, Mathews CE, Bunn HF, Stehno-Bittel L, Zhu H Abstract NADH-cytochrome b5 oxidoreductase (Ncb5or) is an endoplasmic reticulum (ER)-associated redox enzyme involved in fatty acid metabolism, and phenotypic abnormalities of Ncb5or(-/-) mice include diabetes and lipoatrophy. These mice are lean and insulin-sensitive but become hyperglycemic at age 7weeks as a result of β-cell dysfunction and loss. Here we examine early cellular and molecular events associated with manifestations of β-cell defects in Ncb5or(-/-) mice. We observe lower islet β-cell content in pancreata at age 4weeks and prominent ER distention in β-cells by age 5weeks. Ultrastructural changes progre... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294271 Sat, 08 Oct 2011 02:16:21 +0100 5294271 http://www.medworm.com/index.php?rid=5294270&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21840395%26dopt%3DAbstract Authors: Anstee QM, Daly AK, Day CP Abstract Non-alcoholic fatty liver disease (NAFLD) is now recognised as the most common cause of liver dysfunction worldwide. However, whilst the majority of individuals who exhibit features of the metabolic syndrome including obesity and insulin resistance will develop steatosis, only a minority progress to steatohepatitis, fibrosis and cirrhosis. Subtle inter-patient genetic variations and environment interact to determine disease phenotype and influence progression. A decade after the sequencing of the human genome, the comprehensive study of genomic variation offers new insights into the modifier genes, pathogenic mechanisms and is beginning to suggest novel therapeutic targets. We review the current status of the field with particular focus ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294270 Sat, 08 Oct 2011 02:16:09 +0100 5294270 http://www.medworm.com/index.php?rid=5294269&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21856415%26dopt%3DAbstract Authors: Guergnon J, Godet AN, Galioot A, Falanga PB, Colle JH, Cayla X, Garcia A Abstract Protein phosphatase 2A (PP2A) is a large family of holoenzymes that comprises 1% of total cellular proteins and accounts for the majority of Ser/Thr phosphatase activity in eukaryotic cells. Although initially viewed as constitutive housekeeping enzymes, it is now well established that PP2A proteins represent a family of highly and sophistically regulated phosphatases. The past decade, multiple complementary studies have improved our knowledge about structural and functional regulation of PP2A holoenzymes. In this regard, after summarizing major cellular regulation, this review will mainly focus on discussing a particulate biological strategy, used by various viruses, which is based on the ta... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294269 Sat, 08 Oct 2011 02:15:58 +0100 5294269 http://www.medworm.com/index.php?rid=5294268&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21867751%26dopt%3DAbstract Authors: Verwaest KA, Vu TN, Laukens K, Clemens LE, Nguyen HP, Van Gasse B, Martins JC, Van Der Linden A, Dommisse R Abstract Huntington disease (HD) is a hereditary brain disease. Although the causative gene has been found, the exact mechanisms of the pathogenesis are still unknown. Recent investigations point to metabolic and energetic dysfunctions in HD neurons. Both univariate and multivariate analyses were used to compare proton nuclear magnetic resonance spectra of serum and cerebrospinal fluid (CSF) taken from presymptomatic HD transgenic rats and their wild-type littermates. N-acetylaspartate (NAA), was found to be significantly decreased in the serum of HD rats compared to wild-type littermates. Moreover, in the serum their levels of glutamine, succinic acid, glucose and l... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294268 Sat, 08 Oct 2011 02:15:46 +0100 5294268 http://www.medworm.com/index.php?rid=5294267&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21871956%26dopt%3DAbstract Authors: Mao P, Reddy PH Abstract Alzheimer's disease (AD) is an age-related progressive neurodegenerative disease affecting thousands of people in the world and effective treatment is still not available. Over two decades of intense research using AD postmortem brains, transgenic mouse and cell models of amyloid precursor protein and tau revealed that amyloid beta (Aβ) and hyperphosphorylated tau are synergistically involved in triggering disease progression. Accumulating evidence also revealed that aging and amyloid beta-induced oxidative DNA damage and mitochondrial dysfunction initiate and contributes to the development and progression of the disease. The purpose of this article is to summarize the latest progress in aging and AD, with a special emphasis on the mitochondria, o... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294267 Sat, 08 Oct 2011 02:15:35 +0100 5294267 http://www.medworm.com/index.php?rid=5294266&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21889589%26dopt%3DAbstract Authors: Kranendijk M, Salomons GS, Gibson KM, Van Schaftingen E, Jakobs C, Struys EA Abstract The recent discovery of heterozygous isocitrate dehydrogenase 2 (IDH2) mutations of residue Arg(140) to Gln(140) or Gly(140) (IDH2(wt/R140Q), IDH2(wt/R140G)) in d-2-hydroxyglutaric aciduria (D-2-HGA) has defined the primary genetic lesion in 50% of D-2-HGA patients, denoted type II. Overexpression studies with IDH1(R132H) and IDH2(R172K) mutations demonstrated that the enzymes acquired a new function, converting 2-ketoglutarate (2-KG) to d-2-hydroxyglutarate (D-2-HG), in lieu of the normal IDH reaction which reversibly converts isocitrate to 2-KG. To confirm the IDH2(wt/R140Q) gain-of-function in D-2-HGA type II, and to evaluate potential therapeutic strategies, we developed a specific an... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294266 Sat, 08 Oct 2011 02:15:25 +0100 5294266 http://www.medworm.com/index.php?rid=5294265&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21907281%26dopt%3DAbstract Authors: Martins JR, Kongsuphol P, Sammels E, Dahimène S, Aldehni F, Clarke LA, Schreiber R, de Smedt H, Amaral MD, Kunzelmann K Abstract In many cells, increase in intracellular calcium ([Ca(2+)](i)) activates a Ca(2+)-dependent chloride (Cl(-)) conductance (CaCC). CaCC is enhanced in cystic fibrosis (CF) epithelial cells lacking Cl(-) transport by the CF transmembrane conductance regulator (CFTR). Here, we show that in freshly isolated nasal epithelial cells of F508del-homozygous CF patients, expression of TMEM16A and bestrophin 1 was unchanged. However, calcium signaling was strongly enhanced after induction of expression of F508del-CFTR, which is unable to exit the endoplasmic reticulum (ER). Since receptor-mediated [Ca(2+)](i) increase is Cl(-) dependent, we suggested that F5... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5294265 Sat, 08 Oct 2011 02:15:14 +0100 5294265 http://www.medworm.com/index.php?rid=5241113&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21924992%26dopt%3DAbstract Authors: Tzivion G, Hay N PMID: 21924992 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5241113 Thu, 22 Sep 2011 18:56:14 +0100 5241113 http://www.medworm.com/index.php?rid=5189612&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21884966%26dopt%3DAbstract Authors: Wilson PD PMID: 21884966 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5189612 Sat, 03 Sep 2011 23:36:09 +0100 5189612 http://www.medworm.com/index.php?rid=5189613&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21870359%26dopt%3DAbstract Retraction notice to "Statin blocks Rho/Rho-kinase signalling and disrupts the actin cytoskeleton: relationship to enhancement of LPS-mediated nitric oxide synthesis in vascular smooth muscle cells" [Biochem.Biophys.Acta 1689(2004)267-72]. Biochim Biophys Acta. 2011 Sep;1812(9):1200 Authors: PMID: 21870359 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5189613 Wed, 31 Aug 2011 23:00:00 +0100 5189613 http://www.medworm.com/index.php?rid=5171336&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21530691%26dopt%3DAbstract Authors: Falahati M, Ma'mani L, Saboury AA, Shafiee A, Foroumadi A, Badiei AR Abstract In this research, the immobilization of superoxide dismutase (SOD) onto aminopropyl-functionalized KIT-6 [n-PrNH(2)-KIT-6] was investigated. This organo-functionalized mesoporous silica nanoparticle was prepared using a non-ionic surfactant and was fully characterized by XRD, nitrogen adsorption-desorption isotherm assay, IR and TGA techniques. An activity assay demonstrated that the immobilized SOD had a higher activity than the free enzyme. Further investigations using FT-IR, circular dichroism (CD), and probe 1-anilino-8-naphthalene sulfonate (ANS) fluorescence intensity measurements indicated that the structure of the enzyme did not change upon binding to the mesoporous silica, and that immob... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171336 Mon, 29 Aug 2011 19:09:32 +0100 5171336 http://www.medworm.com/index.php?rid=5171335&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21570497%26dopt%3DAbstract Authors: Oppezzo P, Obal G, Baraibar MA, Pritsch O, Alzari PM, Buschiazzo A Abstract Trans-sialidases are surface-located proteins in Trypanosoma cruzi that participate in key parasite-host interactions and parasite virulence. These proteins are encoded by a large multigenic family, with tandem-repeated and individual genes dispersed throughout the genome. While a large number of genes encode for catalytically active enzyme isoforms, many others display mutations that involve catalytic residues. The latter ultimately code for catalytically inactive proteins with very high similarity to their active paralogs. These inactive members have been shown to be lectins, able to bind sialic acid and galactose in vitro, although their cellular functions are yet to be fully established. We now... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171335 Mon, 29 Aug 2011 19:09:26 +0100 5171335 http://www.medworm.com/index.php?rid=5171334&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21586349%26dopt%3DAbstract Authors: Falero A, Caballero A, Trigueros S, Pérez C, Campos J, Marrero K, Fando R Abstract pV(VGJΦ), a single-stranded DNA binding protein of the vibriophage VGJΦ was subject to biochemical analysis. Here, we show that this protein has a general affinity for single-stranded DNA (ssDNA) as documented by Electrophoretic Mobility Shift Assay (EMSA). The apparent molecular weight of the monomer is about 12.7kDa as measured by HPLC-SEC. Moreover, isoelectrofocusing showed an isoelectric point for pV(VGJΦ) of 6.82 pH units. Size exclusion chromatography in 150mM NaCl, 50mM sodium phosphate buffer, pH 7.0 revealed a major protein species of 27.0kDa, suggesting homodimeric protein architecture. Furthermore, pV(VGJΦ) binds ssDNA at extreme temperatures and the complex was stable after... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171334 Mon, 29 Aug 2011 19:09:21 +0100 5171334 http://www.medworm.com/index.php?rid=5171333&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21600316%26dopt%3DAbstract Authors: Juillard S, Chevance S, Bondon A, Simonneaux G Abstract The asymmetric 3-ethyl-2-methylporphyrin iron complex was synthetized and inserted into apomyoglobin. UV-visible spectroscopic studies demonstrated the capacity of iron to coordinate different exogenous axial ligands in ferrous and ferric forms. The position of synthetic heme into the hydrophobic pocket of the reconstituted myoglobin was investigated by ((1))H NMR spectroscopy. In absence of exogenous ligand, signals of the synthetic prosthetic group were not detected, suggesting a rotational disorder of the synthetic porphyrin into the heme pocket. This direct interconversion behavior is favored since site-specific interactions between the poorly substituted heme and protein in the chiral hydrophobic cavity were weak... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171333 Mon, 29 Aug 2011 19:09:15 +0100 5171333 http://www.medworm.com/index.php?rid=5171332&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21600318%26dopt%3DAbstract Authors: Dergunov AD Abstract GuHCl-induced denaturation of human plasma apoA-I, apoA-II, apoA-IV, apoE3 and three recombinant apoE isoforms in solution and discoidal complexes with phosphatidylcholine (only plasma proteins) was studied. The protein conformational stability (ΔG(H(2)O)) and a slope of linear dependence of free energy of unfolding on GuHCl concentration (m-value) were estimated with the three equilibrium schemes. The data for all proteins, except apoA-II, fit with the three-state model, thus evidencing two-domain structure. The predicted folding rate of the four apoE in solution correlated with conformational stability. The dependence disappeared at the inclusion of apoA-I and apoA-IV into analysis and the m-values, adjusted for residue number in helices (m(rh)), di... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171332 Mon, 29 Aug 2011 19:09:09 +0100 5171332 http://www.medworm.com/index.php?rid=5171331&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21601016%26dopt%3DAbstract Authors: Kumagai Y, Usuki H, Yamamoto Y, Yamasato A, Arima J, Mukaihara T, Hatanaka T Abstract Despite the widespread industrial applications of β-mannanase, the relations between the enzymatic properties and metal ions remain poorly understood. To elucidate the effects of metal ions on β-mannanase, thermal stability and hydrolysis activity were characterized. The stman and tfman genes encoding β-mannanase (EC.3.2.1.78) from Streptomyces thermolilacinus NBRC14274 and Thermobifida fusca NBRC14071 were cloned and expressed in Escherichia coli. The thermal stability of each enzyme shifted to the 7-9°C high temperature in the presence of Ca(2+) compared with that in the absence of Ca(2+). These results show that the thermal stability of StMan and TfMan was enhanced by the presence ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171331 Mon, 29 Aug 2011 19:09:03 +0100 5171331 http://www.medworm.com/index.php?rid=5171330&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21609790%26dopt%3DAbstract This study shows that protein kinase A (PKA) phosphorylates SMN both in vitro and in vivo. Bioinformatic analysis predicts 12 potential PKA phosphorylation sites in human SMN. Mass spectrometric analysis of a tryptic digest of SMN after PKA phosphorylation identified five distinct phosphorylation sites in SMN (serines 4, 5, 8, 187 and threonine 85). Mutagenesis of this subset of PKA-phosphorylated sites in SMN affects association of SMN with Gemin2 and Gemin8. This result indicates that phosphorylation of SMN by PKA may play a role in regulation of the in vivo function of SMN. PMID: 21609790 [PubMed - indexed for MEDLINE] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171330 Mon, 29 Aug 2011 19:08:57 +0100 5171330 http://www.medworm.com/index.php?rid=5171329&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21616177%26dopt%3DAbstract Authors: Hudson AO, Klartag A, Gilvarg C, Dobson RC, Marques FG, Leustek T Abstract Bacteroides fragilis and Clostridium thermocellum were recently found to synthesize diaminopimelate (DAP) by way of LL-DAP aminotransferase. Both species also contain an ortholog of meso-diaminopimelate dehydrogenase (Ddh), suggesting that they may have redundant pathways for DAP biosynthesis. The B. fragilis Ddh ortholog shows low homology with other examples of Ddh and this species belongs to a phylum, the Bacteriodetes, not previously known to contain this enzyme. By contrast, the C. thermocellum ortholog is well conserved with known examples of Ddh. Using in vitro and in vivo assays both the B. fragilis and C. thermocellum enzymes were found to be authentic examples of Ddh, displaying kinetic pr... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171329 Mon, 29 Aug 2011 19:08:51 +0100 5171329 http://www.medworm.com/index.php?rid=5171328&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21616178%26dopt%3DAbstract In this study, a combined two-dimensional gel electrophoresis and mass spectrometry approach was utilized to determine the proteome profile of soybean seeds (Glycine max L.; Eunhakong). Comparative analysis showed that the temporal profiles of protein expression are dramatically changed during the seed germination and seedling growth. More than 80% of the proteins identified were subunits of glycinin and β-conglycinin, two major storage proteins. Most subunits of these proteins were degraded almost completely at a different rate by 120h, and the degradation products were accumulated or degraded further. Interestingly, the acidic subunits of glycinin were rapidly degraded, but no obvious change in the basic chains. Of the five acidic subunits, the degradation of G2 subunit was not apparent... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171328 Mon, 29 Aug 2011 19:08:46 +0100 5171328 http://www.medworm.com/index.php?rid=5171327&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21621013%26dopt%3DAbstract Authors: Wezner-Ptasińska M, Krowarsch D, Otlewski J Abstract Variable lymphocyte receptors (VLRs) serve as antigen binding proteins in jawless vertebrates. Their relatively low molecular weight makes VLRs an interesting alternative to antibodies in biotechnological applications. A typical VLR comprises several unique motifs called leucine-rich repeats (LRRs). Using consensus approach we designed a novel VLR protein (called dVLR) containing six LRR repeats based on a sea lamprey receptor sequence. The designed protein was expressed in Escherichia coli in a soluble, native form and showed very favorable biophysical properties. Recombinant dVLR is monomeric in solution and preserves its secondary structure within the pH range 3.0 to 11.0 and tertiary structure between pH 4.0 and 10.... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171327 Mon, 29 Aug 2011 19:08:41 +0100 5171327 http://www.medworm.com/index.php?rid=5171326&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21621014%26dopt%3DAbstract Authors: Seneviratne AM, Burroughs M, Giralt E, Smrcka AV Abstract Heterotrimeric guanine nucleotide-binding proteins (G proteins) composed of three subunits α, β, γ mediate activation of multiple intracellular signaling cascades initiated by G protein-coupled receptors (GPCRs). Previously our laboratory identified small molecules that bind to Gβγ and interfere with or enhance binding of select effectors with Gβγ. To understand the molecular mechanisms of selectivity and assess binding of compounds to Gβγ, we used biophysical and biochemical approaches to directly monitor small molecule binding to Gβγ. Surface plasmon resonance (SPR) analysis indicated that multiple compounds bound directly to Gβγ with affinities in the high nanomolar to low micromolar range but with s... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171326 Mon, 29 Aug 2011 19:08:36 +0100 5171326 http://www.medworm.com/index.php?rid=5171325&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21621654%26dopt%3DAbstract Authors: Vandevenne M, Gaspard G, Belgsir el M, Ramnath M, Cenatiempo Y, Marechal D, Dumoulin M, Frere JM, Matagne A, Galleni M, Filee P Abstract Irreversible accumulation of protein aggregates represents an important problem both in vivo and in vitro. The aggregation of proteins is of critical importance in a wide variety of biomedical situations, ranging from diseases (such as Alzheimer's and Parkinson's diseases) to the production (e.g. inclusion bodies), stability, storage and delivery of protein drugs. β-Cyclodextrin (β-CD) is a circular heptasaccharide characterized by a hydrophilic exterior and a hydrophobic interior ring structure. In this research, we studied the effects of a chemically modified β-CD (BCD07056), on the aggregating and refolding properties of BlaPChBD, a... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171325 Mon, 29 Aug 2011 19:08:30 +0100 5171325 http://www.medworm.com/index.php?rid=5171324&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21621655%26dopt%3DAbstract Authors: Bertoldo JB, Razzera G, Vernal J, Brod FC, Arisi AC, Terenzi H Abstract Lipases are well-known enzymes extensively used in industrial biotransformation processes. Besides, their structural and catalytic characteristics have attracted increasing attention of several industries in the last years. In this work, we used biophysical and molecular modeling tools to assess structural properties of Staphylococcus xylosus lipase (SXL). We studied the thermal unfolding of this protein and its zinc-dependent thermotolerance. We demonstrated that SXL is able to be active and stable at moderate temperatures, but this feature is only acquired in the presence of Zn(2+). Such characteristic indicates SXL as a zinc-dependent metallolipase. PMID: 21621655 [PubMed - indexed for MEDLINE] ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171324 Mon, 29 Aug 2011 19:08:25 +0100 5171324 http://www.medworm.com/index.php?rid=5171323&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21640851%26dopt%3DAbstract Authors: Fogle EJ, Toney MD Abstract Diaminopimelate decarboxylase (DAPDC) and ornithine decarboxylase (ODC) are pyridoxal 5'-phosphate dependent enzymes that are critical to microbial growth and pathogenicity. The latter is the target of drugs that cure African sleeping sickness, while the former is an attractive target for antibacterials. These two enzymes share the (β/α)(8) (i.e., TIM barrel) fold with alanine racemase, another pyridoxal 5'-phosphate dependent enzyme critical to bacterial survival. The active site structural homology between DAPDC and ODC is striking even though DAPDC catalyzes the decarboxylation of a D stereocenter with inversion of configuration and ODC catalyzes the decarboxylation of an L stereocenter with retention of configuration. Here, the structural ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171323 Mon, 29 Aug 2011 19:08:19 +0100 5171323 http://www.medworm.com/index.php?rid=5171322&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21645649%26dopt%3DAbstract In this study we used the purified proteins from Azospirillum brasilense to analyze the effect of P(II) uridylylation on the protein's ability to engage complex formation with AmtB in vitro. Our results show that partially uridylylated P(II) trimers can interact with AmtB in vitro, the implication of this finding in the regulation of nitrogen metabolism is discussed. We also report an improved expression and purification protocol for the A. brasilense AmtB protein that might be applicable to AmtB proteins from other organisms. PMID: 21645649 [PubMed - indexed for MEDLINE] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5171322 Mon, 29 Aug 2011 19:08:12 +0100 5171322 http://www.medworm.com/index.php?rid=5139265&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F08%252F03%252023.15%2522%255BMHDA%255D%253A%25222011%252F08%252F19%252007.35%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F08%252F03%252023.15%2522%255BEDAT%255D%29%29 190 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/08/19PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5139265 Fri, 19 Aug 2011 11:35:02 +0100 5139265 http://www.medworm.com/index.php?rid=5090254&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F07%252F20%252012.15%2522%255BMHDA%255D%253A%25222011%252F08%252F03%252023.15%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F07%252F20%252012.15%2522%255BEDAT%255D%29%29 171 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/08/03PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=5090254 Thu, 04 Aug 2011 03:15:02 +0100 5090254 http://www.medworm.com/index.php?rid=4947588&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21641495%26dopt%3DAbstract Authors: Di Lisa F, Schulz R, Murphy E PMID: 21641495 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947588 Mon, 20 Jun 2011 21:46:08 +0100 4947588 http://www.medworm.com/index.php?rid=4947586&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21664556%26dopt%3DAbstract Authors: Moschetta A PMID: 21664556 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947586 Mon, 20 Jun 2011 21:46:00 +0100 4947586 http://www.medworm.com/index.php?rid=4947581&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21672638%26dopt%3DAbstract Authors: Fujii T, Shimizu M, Doi Y, Fujita T, Ito T, Miura D, Wariishi H, Takaya N We discovered the phenyllactate (PLA)-producing fungal strain Wickerhamia fluorescens TK1 and purified phenylpyruvate reductase (PPR) from fungal cell-free extracts. The PPR used both NADPH and NADH as cofactors with more preference for the former. The enzyme reaction as well as the fungal culture produced optically active d-PLA. The gene for the PPR (pprA) was cloned and expressed in Escherichia coli cells. Purified preparations of both native and recombinant PPR used hydroxyphenylpyruvate, glyoxylate and hydroxypyruvate as substrates but not pyruvate, oxaloacetate or benzoylformate. The predicted PPR protein had sequence similarity to proteins in the d-isomer-specific 2-hydroxyacid dehydrogenase family... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947581 Fri, 10 Jun 2011 23:00:00 +0100 4947581 http://www.medworm.com/index.php?rid=4947580&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21672639%26dopt%3DAbstract Authors: Rather LJ, Bill E, Ismail W, Fuchs G BoxA is the reductase component of the benzoyl-coenzyme A (CoA) oxidizing epoxidase enzyme system BoxAB. The enzyme catalyzes the key step of an hitherto unknown aerobic, CoA-dependent pathway of benzoate metabolism, which is the epoxidation of benzoyl-CoA to the non-aromatic 2,3-epoxybenzoyl-CoA. The function of BoxA is the transfer of two electrons from NADPH to the epoxidase component BoxB. We could show recently that BoxB is a diiron enzyme, whereas here we demonstrate that BoxA harbors an FAD and two [4Fe-4S] clusters per protein monomer. The characterization of BoxA was hampered by severe oxygen sensitivity; the cubane [4Fe-4S] clusters degrade already with traces of oxygen. Interestingly, the adventitiously formed [3Fe-4S] centers co... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947580 Fri, 10 Jun 2011 23:00:00 +0100 4947580 http://www.medworm.com/index.php?rid=4947583&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669299%26dopt%3DAbstract This article is part of a Special Issue entitled Retinoid and Lipid Metabolism. PMID: 21669299 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947583 Thu, 09 Jun 2011 23:00:00 +0100 4947583 http://www.medworm.com/index.php?rid=4947587&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21664431%26dopt%3DAbstract CONCLUSIONS: These results show that E5 causes the retention of its fused protein inside endosomes even after lysis takes place. GENERAL SIGNIFICANCE: These data establish that HA2 analogs might not be useful PTDs unless cleavable linkers are engineered between PTD and protein cargo. PMID: 21664431 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947587 Tue, 07 Jun 2011 23:00:00 +0100 4947587 http://www.medworm.com/index.php?rid=4947585&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669182%26dopt%3DAbstract Authors: Schansker G, Tóth SZ, Kovács L, Holzwarth AR, Garab G Experiments were carried out to identify a process co-determining with Q(A) the fluorescence rise between F(0) and F(M). With 3-(3',4'-dichlorophenyl)-1,1-dimethylurea (DCMU), the fluorescence rise is sigmoidal, in its absence it is not. Lowering the temperature to -10°C the sigmoidicity is lost. It is shown that the sigmoidicity is due to the kinetic overlap between the reduction kinetics of Q(A) and a second process; an overlap that disappears at low temperature because the temperature dependences of the two processes differ. This second process can still relax at -60°C where recombination between Q(A)(-) and the donor side of photosystem (PS) II is blocked. This suggests that it is not a redox reaction but a conforma... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947585 Mon, 06 Jun 2011 23:00:00 +0100 4947585 http://www.medworm.com/index.php?rid=4947584&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669184%26dopt%3DAbstract Authors: Hunke C, Antosch M, Müller V, Grüber G Two of the distinct diversities of the engines A(1)A(O) ATP synthase and F(1)F(O) ATP synthase are the existence of two peripheral stalks and the 24kDa stalk subunit E inside the A(1)A(O) ATP synthase. Crystallographic structures of subunit E have been determined recently, but the epitope(s) and the strength to which this subunit does bind in the enzyme complex are still a puzzle. Using the recombinant A(3)B(3)D complex and the major subunits A and B of the methanogenic A(1)A(O) ATP synthase in combination with fluorescence correlation spectroscopy (FCS) we demonstrate, that the stalk subunit E does bind to the catalytic headpiece formed by the A(3)B(3) hexamer with an affinity (K(d)) of 6.1±0.2μM. FCS experiments with single A and B,... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947584 Mon, 06 Jun 2011 23:00:00 +0100 4947584 http://www.medworm.com/index.php?rid=4947582&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21672514%26dopt%3DAbstract Authors: Sánchez-Martín MJ, Busquets MA, Girona V, Haro I, Alsina MA, Pujol M One way to gain information about the fusogenic potential of virus-derived synthetic peptides is to examine their interfacial properties and subsequently to study them in monolayers and bilayers. Here, we characterize the physicochemical surface properties of the peptide E1(64-81), whose sequence is AQLVGELGSLYGPLSVSA. This peptide is derived from the E1 structural protein of GBV-C/HGV which was previously shown to inhibit leakage of vesicular contents caused by the HIV-1 fusion peptide (HIV-1 FP). Mixed isotherms of E1(64-81) and HIV-1 FP were obtained and their Brewster angle microscopy (BAM) and atomic force microscopy (AFM) images showed that the peptide mixture forms a different structure that is not p... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947582 Mon, 06 Jun 2011 23:00:00 +0100 4947582 http://www.medworm.com/index.php?rid=4947579&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21679683%26dopt%3DAbstract Authors: Seelert H, Dencher NA ATP synthases are part of the sophisticated cellular metabolic network and therefore multiple interactions have to be considered. As discussed in this review, ATP synthases form various supramolecular structures. These include dimers and homooligomeric species, but also interactions with other proteins, particularly those involved in energy conversion. The supramolecular assembly of the ATP synthase affects metabolism, organellar structure, diseases and ageing. The most common approaches to isolate supercomplexes from native membranes by use of native electrophoresis or density gradients are introduced. On the one hand, isolated ATP synthase dimers and oligomers are employed for structural studies and elucidation of specific protein-protein interactions. ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947579 Mon, 06 Jun 2011 23:00:00 +0100 4947579 http://www.medworm.com/index.php?rid=4947578&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21679781%26dopt%3DAbstract This article is part of a Special Issue entitled: MicroRNAs in viral gene regulation. PMID: 21679781 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4947578 Mon, 06 Jun 2011 23:00:00 +0100 4947578 http://www.medworm.com/index.php?rid=4899279&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F05%252F23%252009.45%2522%255BMHDA%255D%253A%25222011%252F06%252F06%252010.45%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F05%252F23%252009.45%2522%255BEDAT%255D%29%29 106 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/06/06PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4899279 Mon, 06 Jun 2011 14:45:02 +0100 4899279 http://www.medworm.com/index.php?rid=4851463&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F05%252F08%252019.30%2522%255BMHDA%255D%253A%25222011%252F05%252F23%252009.45%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F05%252F08%252019.30%2522%255BEDAT%255D%29%29 127 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/05/23PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4851463 Mon, 23 May 2011 13:45:02 +0100 4851463 http://www.medworm.com/index.php?rid=4798369&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F03%252F26%252003.45%2522%255BMHDA%255D%253A%25222011%252F05%252F08%252019.30%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F03%252F26%252003.45%2522%255BEDAT%255D%29%29 270 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/05/08PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4798369 Sun, 08 May 2011 23:30:05 +0100 4798369 http://www.medworm.com/index.php?rid=4636184&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F03%252F11%252017.00%2522%255BMHDA%255D%253A%25222011%252F03%252F26%252003.45%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F03%252F11%252017.00%2522%255BEDAT%255D%29%29 167 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/03/26PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4636184 Sat, 26 Mar 2011 07:45:03 +0100 4636184 http://www.medworm.com/index.php?rid=4573701&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F02%252F25%252010.45%2522%255BMHDA%255D%253A%25222011%252F03%252F11%252017.00%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F02%252F25%252010.45%2522%255BEDAT%255D%29%29 204 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/03/11PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4573701 Fri, 11 Mar 2011 22:00:04 +0100 4573701 http://www.medworm.com/index.php?rid=4518206&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21316607%26dopt%3DAbstract Authors: Blatch GL, Przyborski JM PMID: 21316607 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518206 Fri, 25 Feb 2011 15:46:56 +0100 4518206 http://www.medworm.com/index.php?rid=4518205&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21316608%26dopt%3DAbstract Authors: PMID: 21316608 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518205 Fri, 25 Feb 2011 15:46:51 +0100 4518205 http://www.medworm.com/index.php?rid=4518225&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315685%26dopt%3DAbstract Authors: Sharma V, Wikström M, Kaila VR The proton-pumping cbb(3)-type cytochrome c oxidases catalyze cell respiration in many pathogenic bacteria. For reasons not yet understood, the apparent dioxygen (O(2)) affinity in these enzymes is very high relative to other members of the heme-copper oxidase (HCO) superfamily. Based on density functional theory (DFT) calculations on intermediates of the oxygen scission reaction in active-site models of cbb(3)- and aa(3)-type oxidases, we find that a transient peroxy intermediate (I(P), Fe[III]-OOH(-)) is ~6kcal/mol more stable in the former case, resulting in more efficient kinetic trapping of dioxygen and hence in a higher apparent oxygen affinity. The major molecular basis for this stabilization is a glutamate residue, polarizing the proxima... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518225 Sun, 20 Feb 2011 00:00:00 +0100 4518225 http://www.medworm.com/index.php?rid=4518221&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315776%26dopt%3DAbstract Authors: von Roretz C, Beauchamp P, Di Marco S, Gallouzi IE The process of muscle cell differentiation into myotubes, termed myogenesis, depends on a complex coordination of myogenic factors, many of which are regulated post-transcriptionally. HuR, an mRNA-binding protein, is responsible for regulating the expression of several such myogenic factors by stabilizing the mRNAs encoding these factors. The critical role for HuR in myogenesis also involves the nucleocytoplasmic shuttling abilities of this protein. Indeed, in order to perform its stabilizing functions, HuR must accumulate in the cytoplasm. This requires its dissociation from the import factor Transportin 2 (TRN2) which is actually caused by the cleavage of a portion of cytoplasmic HuR. In this review, we describe the roles of... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518221 Sun, 20 Feb 2011 00:00:00 +0100 4518221 http://www.medworm.com/index.php?rid=4518181&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21342666%26dopt%3DAbstract In this report we define the core promoter functional elements in the G. mellonella jhbp gene. It appears that jhbp core promoter is under strong control of TATA box and the transcription site (tss). In contrast to regulation of the jhe gene, the jhbp core promoter contains a sequence which directly suppresses jhbp expression. Evidences are provided for the contribution of the will die slowly (WDS) suppressory protein in jhbp basal transcription. We have also shown that the activity of the jhbp core promoter can be regulated by JHIII, and lesser so by 20E. PMID: 21342666 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518181 Sat, 19 Feb 2011 00:00:00 +0100 4518181 http://www.medworm.com/index.php?rid=4518222&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315775%26dopt%3DAbstract We present a study of intramuscular motion during contraction of skeletal muscle myofibrils. Myofibrillar actin was labeled with fluorescent dye so that the ratio of fluorescently labeled to unlabeled protein was 1:10(5). Such sparse labeling assured that there was on average only one actin-marker present in the focus at a given time. From the intensity signal in the two orthogonal detection channels, significant fluctuations, similar to fluorescent burst in diffusion-based single-molecule detection schemes, were identified via a threshold algorithm and analyzed with respect to their intensity and polarization. When only rigor complexes were formed, the fluctuations of polarized intensity were characterized by unimodal Gaussian photon distributions. During contraction, in contrast, bimodal... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518222 Fri, 18 Feb 2011 00:00:00 +0100 4518222 http://www.medworm.com/index.php?rid=4518219&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315804%26dopt%3DAbstract This article is part of a Special Issue entitled: Biochemistry of Asthma. PMID: 21315804 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518219 Fri, 18 Feb 2011 00:00:00 +0100 4518219 http://www.medworm.com/index.php?rid=4518213&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315851%26dopt%3DAbstract Authors: Winiarska B, Dwornik A, Dębski J, Grzelak K, Bystranowska D, Zalewska M, Dadlez M, Ożyhar A, Kochman M Juvenile hormone (JH) regulates insect growth and development. JH present in the hemolymph is bound to juvenile hormone binding protein (hJHBP) which protects JH from degradation. In G. mellonella, this protein is glycosylated only at one (Asn(94)) of the two potential N-linked glycosylation sites (Asn(4) and Asn(94)). To investigate the function of glycosylation, each of the two potential glycosylation sites in the rJHBP molecule was examined by site-directed mutagenesis. MS analysis revealed that rJHBP overexpressed in the P. pastoris system may appear in a non-glycosylated as well as in a glycosylated form at both sites. We found that mutation at position Asn(94) reduces... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518213 Fri, 18 Feb 2011 00:00:00 +0100 4518213 http://www.medworm.com/index.php?rid=4518212&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315852%26dopt%3DAbstract This article is part of a Special Issue entitled: Pyridoxal Phosphate Enzymology. PMID: 21315852 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518212 Fri, 18 Feb 2011 00:00:00 +0100 4518212 http://www.medworm.com/index.php?rid=4518211&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315853%26dopt%3DAbstract This article is part of a Special Issue entitled: Pyridoxal Phospate Enzymology. PMID: 21315853 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518211 Fri, 18 Feb 2011 00:00:00 +0100 4518211 http://www.medworm.com/index.php?rid=4518199&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320573%26dopt%3DAbstract Authors: Chang WQ, Wu XZ, Cheng AX, Zhang L, Ji M, Lou HX BACKGROUND: Retigeric acid B (RAB), a triterpene acid isolated from Lobaria kurokawae exerts antifungal effect. The present study was designed to elucidate the underlying mechanisms by which RAB regulates the proliferation and cell death of Candida albicans. METHODS: We measured the metabolic activity of C. albicans with WST1 Cell Proliferation and Cytotoxicity Assay Kit, analyzed the cell cycle by flow cytometry, visualized the ultrastructure by transmission electron microscopy (TEM) and investigated the apoptosis and necrosis induced by RAB using confocal microscopy. The reactive oxygen species (ROS) accumulation was determined by spectrophotometry, flow cytometry and fluorescent microscopy. The mtΔψ was detected using flow ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518199 Fri, 18 Feb 2011 00:00:00 +0100 4518199 http://www.medworm.com/index.php?rid=4518198&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320574%26dopt%3DAbstract CONCLUSIONS: These results suggest that (1) the structural integrity of aortic tissue at 4°C is retained for at least the first three days after resection and (2) OCT is a powerful technology well suited for evaluating tissue structural integrity over time. GENERAL SIGNIFICANCE: Functional OCT imaging provides for a noninvasive and quantitative technique in determining the structural integrity of aortic tissue stored at 4°C. This modality may be used for assessing the efficacy of other preservation techniques. PMID: 21320574 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518198 Fri, 18 Feb 2011 00:00:00 +0100 4518198 http://www.medworm.com/index.php?rid=4518185&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21338628%26dopt%3DAbstract Authors: Markovic D, Punn A, Lehnert H, Grammatopoulos DK In most target tissues, the adenylyl cyclase/cAMP/PKA, the extracellular signal regulated kinase (ERK1/2) and the PKB/Akt are the main pathways employed by the type 2 corticotropin-releasing hormone (CRH) receptor to mediate the biological actions of urocortins (Ucns) and CRH. To decipher the molecular determinants of CRH-R2 signaling, we studied the signaling pathways in HEK293 cells overexpressing recombinant human CRH-R2ß receptors. Use of specific kinase inhibitors showed that the CRH-R2ß cognate agonist, Ucn2, activated ERK1/2 in a PI3-K and cAMP/PKA-dependent manner with contribution from Epac activation. Ucn2 also induced PKA-dependent association between AKAP250 and CRH-R2ß that appeared to be necessary for ERK1/2 act... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518185 Fri, 18 Feb 2011 00:00:00 +0100 4518185 http://www.medworm.com/index.php?rid=4518184&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21338656%26dopt%3DAbstract Authors: Nys K, Maes H, Dudek AM, Agostinis P The hypoxia inducible factor-1a (HIF-1a) is a pleiotropic transcription factor typically activated in response to low oxygen tension as well as other stress factors in normoxic conditions. Upon activation HIF-1a mediates the transcriptional activation of target genes involved in a variety of processes comprising stress adaptation, metabolism, growth and invasion, but also apoptotic cell death. The molecular mechanisms, signaling pathways and downstream targets evoked by the activation of HIF-1a in epidermal cells are becoming increasingly understood and underscore the participation of HIF-1α in crucial processes including malignant transformation and cancer progression. Recent studies have implicated HIF-1a as integral part of the multifac... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518184 Fri, 18 Feb 2011 00:00:00 +0100 4518184 http://www.medworm.com/index.php?rid=4518183&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21338716%26dopt%3DAbstract Authors: Raucci R, Colonna G, Guerriero E, Capone F, Accardo M, Castello G, Costantini S ► SELENBP1 belongs to ''selenium binding proteins" family. ► Selenium is an important oligoelement. ► Expression of SELENBP1 in liver tissues of patients with hepatocarcinoma is reduced. ► SELENBP1 is an alpha-beta protein with some intrinsically unordered loop regions. ► Cys57 is the only exposed cysteine with high probability of binding to selenium. PMID: 21338716 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518183 Fri, 18 Feb 2011 00:00:00 +0100 4518183 http://www.medworm.com/index.php?rid=4518182&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21338717%26dopt%3DAbstract Authors: Ines De Laurentiis E, Mo F, Wieden HJ In order to study the structural and functional requirements of the essential translational GTPase Elongation Factor (EF) Tu for efficient and accurate ribosome dependent protein synthesis, construction of a cysteine-free (Cys-less) mutant variant allowing for the site-directed introduction of fluorescent and non-fluorescent labels is of great importance. However, previous reports suggest that a cysteine residue in position 81 of EF-Tu from Escherichia coli is essential for its function. To study the functional role of cysteine 81 and to construct a fully active Cys-less EF-Tu, we have analyzed 125 bacterial sequences with respect to sequence variations in this position revealing that in a small number of sequences alanine and methionine c... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518182 Fri, 18 Feb 2011 00:00:00 +0100 4518182 http://www.medworm.com/index.php?rid=4518230&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21310195%26dopt%3DAbstract In this study, we have examined the molecular mechanism of transcriptional regulation of the IL-12B gene by 1α,25(OH)(2)D(3) in lipopolysaccharide (LPS)-treated human monocytes (THP-1). Quantitative RT-PCR showed that IL-12B mRNA displays a cyclical expression profile and is down-regulated 2.8-fold during the first 8h and even 12.1-fold 24h after exposure to 1α,25(OH)(2)D(3). Gel shift and quantitative chromatin immunoprecipitation (ChIP) assays demonstrated vitamin D receptor (VDR) binding to genomic regions 480 and 6300bp upstream of the IL-12B transcription start site (TSS). Quantitative ChIP assays also revealed that together with VDR and its partner RXR the above regions recruited the co-repressor NCOR2/SMRT and histone deacetylase 3 leading to a decreased histone 4 acetylation and ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518230 Thu, 17 Feb 2011 00:00:00 +0100 4518230 http://www.medworm.com/index.php?rid=4518229&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315062%26dopt%3DAbstract Authors: Potamitis C, Chatzigeorgiou P, Siapi E, Viras K, Mavromoustakos T, Hodzic A, Pabst G, Cacho-Nerin F, Laggner P, Rappolt M Valsartan is a marketed drug with high affinity to the type 1 angiotensin (AT(1)) receptor. It has been reported that AT(1) antagonists may reach the receptor site by diffusion through the plasma membrane. For this reason we have applied a combination of differential scanning calorimetry (DSC), Raman spectroscopy and small and wide angle X-ray scattering (SAXS and WAXS) to investigate the interactions of valsartan with the model membrane of dipalmitoyl-phosphatidylcholine (DPPC). Hence, the thermal, dynamic and structural effects in bulk as well as local dynamic properties in the bilayers were studied with different valsartan concentrations ranging from 0 t... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518229 Thu, 17 Feb 2011 00:00:00 +0100 4518229 http://www.medworm.com/index.php?rid=4518224&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315687%26dopt%3DAbstract Authors: Xin L, Su H, Nielsen CH, Tang C, Torres J, Mu Y Molecular dynamics simulations of aquaporin Z homotetramer which is a membrane protein facilitating rapid water movement through the plasma membrane of Escherichia coli were performed. Initial configurations were taken from the open and closed states of crystal structures separately. The resulting water osmotic permeability (p(f)) and diffusive permeability (p(d)) displayed distinct features. Consistent with previous studies, the side chain conformation of arginine189 was found to mediate the water permeability. A potential of mean force (PMF) as a function of the distance between NH1 of R189 and carbonyl oxygen of A117 was constructed based on the umbrella sampling technique. There are multiple local minima and transition states... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518224 Thu, 17 Feb 2011 00:00:00 +0100 4518224 http://www.medworm.com/index.php?rid=4518223&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315773%26dopt%3DAbstract Authors: Nakachi S, Nakazato T, Ishikawa C, Kimura R, Mann DA, Senba M, Masuzaki H, Mori N Adult T-cell leukemia (ATL) is a T-cell malignancy associated with human T-cell leukemia virus type 1 (HTLV-1) and characterized by visceral invasion. Degradation of the extracellular matrix by matrix metalloproteinases (MMPs) is a crucial process in invasion of tumors and metastasis. MMP-7 (or matrilysin), is a "minimal domain MMP" with proteolytic activity against components of the extracellular matrix. To determine the involvement of MMP-7 in visceral spread in ATL, this study investigated MMP-7 expression in ATL. MMP-7 expression was identified in HTLV-1-infected T-cell lines, peripheral blood ATL cells and ATL cells in lymph nodes, but not in uninfected T-cell lines or normal peripheral bloo... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518223 Thu, 17 Feb 2011 00:00:00 +0100 4518223 http://www.medworm.com/index.php?rid=4518215&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315819%26dopt%3DAbstract Authors: Bueno MJ, Malumbres M The control of cell proliferation by microRNAs (miRNAs) is well established and the alteration of these small, non-coding RNAs may contribute to tumor development by perturbing critical cell cycle regulators. Oncogenic miRNAs may facilitate cell cycle entry and progression by targeting CDK inhibitors or transcriptional repressors of the retinoblastoma family. On the other hand, tumor suppressor miRNAs induce cell cycle arrest by downregulating multiple components of the cell cycle machinery. Recent data also suggest that miRNAs act co-ordinately with transcriptional factors involved in cell cycle regulation such as c-MYC, E2F or p53. These miRNAs not only can potentiate the function of these factors but they may also limit the excessive translation of cel... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518215 Thu, 17 Feb 2011 00:00:00 +0100 4518215 http://www.medworm.com/index.php?rid=4518209&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21316335%26dopt%3DAbstract Authors: Apellániz B, García-Sáez A, Nir S, Nieva JL The human immonodeficiency virus (HIV) envelope is enriched in cholesterol and sphingomyelin, two lipids that sustain the formation of laterally segregated liquid-ordered fluid domains in model systems. Several evidences indicate that the high lipid order existing at the envelope may play a role in HIV pathogenesis. A putative mechanism might involve the modulation of the membrane-perturbing function of the gp41 membrane-proximal external region (MPER). To test such hypothesis, we investigate here the effect of lipid phase coexistence on the membrane-restructuring properties of NpreTM and CpreTM, two peptides based on the amino- and carboxy-terminal MPER sequences, respectively. Fluid phase coexistence elicited the fusogenic activ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518209 Thu, 17 Feb 2011 00:00:00 +0100 4518209 http://www.medworm.com/index.php?rid=4518208&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21316336%26dopt%3DAbstract This article is part of a Special Issue entitled: Adenosine Receptors. PMID: 21316336 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518208 Thu, 17 Feb 2011 00:00:00 +0100 4518208 http://www.medworm.com/index.php?rid=4518207&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21316337%26dopt%3DAbstract This study will provide constraints for future investigations on the interaction of L-selectin and its associating proteins. PMID: 21316337 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518207 Thu, 17 Feb 2011 00:00:00 +0100 4518207 http://www.medworm.com/index.php?rid=4518192&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334303%26dopt%3DAbstract In conclusion, the NMR data provided valuable structural and dynamic insights into the PS-membrane interactions which allow deriving the structural constraints for high membrane affinity and high membrane penetration of a given PS. PMID: 21334303 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518192 Thu, 17 Feb 2011 00:00:00 +0100 4518192 http://www.medworm.com/index.php?rid=4518191&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334304%26dopt%3DAbstract Authors: Foglia F, Drake AF, Terry AE, Rogers SE, Lawrence MJ, Barlow DJ Small angle neutron scattering (SANS) studies have been performed to study the structural changes induced in the membranes of vesicles prepared (by thin film evaporation) from phospholipid and mixed phospholipid-sterol mixtures, in the presence of different concentrations and different aggregation states of the anti-fungal drug, amphotericin B (AmB). In the majority of the experiments reported, the lipid vesicles were prepared with the drug added directly to the lipid dispersions dissolved in solvents favouring either AmB monomers or aggregates, and the vesicles then sonicated to a mean size of ~100 nm. Experiments were also performed, however, in which micellar dispersions of the drug were added to pre-formed lip... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518191 Thu, 17 Feb 2011 00:00:00 +0100 4518191 http://www.medworm.com/index.php?rid=4518190&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334305%26dopt%3DAbstract Authors: Rocafull MA, Romero FJ, Thomas LE, Del Castillo JR Primary Na(+)-transport has been essentially attributed to Na(+)/K(+)-pump. However, there are functional and biochemical evidences that suggest the existence of a K(+)-independent, ouabain-insensitive Na(+)-pump, associated to a Na(+)-ATPase with similar characteristics, located at basolateral plasma membrane of epithelial cells. Herein, membrane protein complex associated with this Na(+)-ATPase was identified. Basolateral membranes from guinea-pig enterocytes were solubilized with polyoxyethylene-9-lauryl-ether and Na(+)-ATPase was purified by concanavalin-A-affinity and ion-exchange chromatographies. Purified enzyme preserves its native biochemical characteristics: Mg(2+)-dependence, specific Na(+)-stimulation, K(+)-indepen... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518190 Thu, 17 Feb 2011 00:00:00 +0100 4518190 http://www.medworm.com/index.php?rid=4518189&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334306%26dopt%3DAbstract In this study we explored the mode of diffusion of the cAMP receptor, cAR1, in the plasma membrane of Dictyostelium discoideum cells and how this is regulated by the cytoskeleton. Single-particle tracking of fluorescently labeled cAR1 using total internal reflection microscopy showed that 70% of the cAR1 molecules were mobile. These receptors showed directed motion and we demonstrate that this is not because of tracking along the actin cytoskeleton. Instead, destabilization of the microtubules abolished cAR1 mobility in the plasma membrane and this was confirmed by fluorescence recovery after photobleaching. As a result of microtubule stabilization, one of the first downstream signaling events, the jump of the PH domain of CRAC, was decreased. These results suggest a role for microtubules ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518189 Thu, 17 Feb 2011 00:00:00 +0100 4518189 http://www.medworm.com/index.php?rid=4518188&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334384%26dopt%3DAbstract Authors: Ramanjaneya M, Conner AC, Brown JE, Chen J, Digby JE, Barber TM, Randeva HS Adiponectin is an abundantly circulating adipokine, orchestrating its effects through two 7-transmembrane receptors (AdipoR1 and AdipoR2). Steroidogenesis is regulated by a variety of neuropeptides and adipokines. Earlier studies have reported adipokine mediated steroid production. A key rate-limiting step in steroidogenesis is cholesterol transportation across the mitochondrial membrane by steroidogenic acute regulatory protein (StAR). Several signalling pathways regulate StAR expression. The actions of adiponectin and its role in human adrenocortical steroid biosynthesis are not fully understood. The aim of this study was to investigate the effects of adiponectin on StAR protein expression, steroidog... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518188 Thu, 17 Feb 2011 00:00:00 +0100 4518188 http://www.medworm.com/index.php?rid=4518187&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334438%26dopt%3DAbstract Authors: El-Hajjaji FZ, Oumeddour A, Pommier AJ, Ouvrier A, Viennois E, Dufour J, Caira F, Drevet JR, Volle DH, Baron S, Saez F, Lobaccaro JM Liver X receptor (LXR) a and LXRb belong to the nuclear receptor superfamily. For many years they have been called orphan receptors, as no natural ligand was identified. In the last decade the LXR natural ligands have been shown to be oxysterols, molecules derived from cholesterol. While these nuclear receptors have been abundantly studied for their roles in the regulation of lipid metabolism, it appears that they also present crucial activities in reproductive organs such as testis and epididymis, as well as prostate. Phenotypic analyses of mice lacking LXRs (lxr-/-) pointed out their physiological activies in the various cells and organs regula... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518187 Thu, 17 Feb 2011 00:00:00 +0100 4518187 http://www.medworm.com/index.php?rid=4518228&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315063%26dopt%3DAbstract Authors: Oard SV α-Hordothionin (αHTH) belongs to thionins, the plant antimicrobial peptides with membrane-permeabilizing activity which is associated with broad-range antimicrobial activity. Experimental data have revealed a phospholipid-binding site and indicated formation of ion channels as well as membrane disruption activity of thionin. However, the mechanism of membrane permeabilization by thionin remained unknown. Here it is shown that thionin is a small water-selective channel. Unbiased high-precision molecular modeling revealed formation of a water-selective pore running through the αHTH double α-helix core when the peptide interacted with anions. Anion-induced unfolding of the C-end of the α2-helix opened a pore mouth. The pore started at the α2 C-end between the hydrop... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518228 Wed, 16 Feb 2011 00:00:00 +0100 4518228 http://www.medworm.com/index.php?rid=4518226&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315150%26dopt%3DAbstract Authors: Leandro J, Leandro P, Flatmark T Hybrid forms of human phenylalanine hydroxylase (hPAH) mutants have been found to present catalytic activities lower than predicted from the individual recombinant forms, indicating that interallelic complementation could be a major determinant of the metabolic phenotypes of compound heterozygous phenylketonuric (PKU) patients. To provide a molecular explanation for interallelic complementation we have here developed a bicistronic expression system and a purification strategy to obtain isolated hPAH heteromeric forms. On co-expression of WT-hPAH (~50% tetramer; ~10% dimer) and the N- and C-terminally truncated form ΔN102/ΔC24-hPAH (~80% dimer) no heterodimers were recovered. Moreover, by co-expression of WT-hPAH and the N-terminally truncated... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518226 Wed, 16 Feb 2011 00:00:00 +0100 4518226 http://www.medworm.com/index.php?rid=4518210&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315854%26dopt%3DAbstract This article is part of a Special Issue entitled: Pyridoxal Phospate Enzymology. PMID: 21315854 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518210 Wed, 16 Feb 2011 00:00:00 +0100 4518210 http://www.medworm.com/index.php?rid=4518194&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21333694%26dopt%3DAbstract Authors: Santamaria R, Fiorito F, Irace C, De Martino L, Maffettone C, Granato GE, Di Pascale A, Iovane V, Pagnini U, Colonna A Cellular iron metabolism is essentially controlled by the binding of cytosolic iron regulatory proteins (IRP1 or IRP2) to iron responsive elements (IREs) located on mRNAs coding for proteins involved in iron acquisition, utilization and storage. The 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is one of the most potent toxins of current interest that occurs as poisonous chemical in the environment. TCDD exposure has been reported to induce a broad spectrum of toxic and biological responses, including significant changes in gene expression for heme and iron metabolism associated with liver injury. Here, we have investigated the molecular effects of TCDD on the ir... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518194 Wed, 16 Feb 2011 00:00:00 +0100 4518194 http://www.medworm.com/index.php?rid=4518193&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21333695%26dopt%3DAbstract Authors: El Btaouri H, Morjani H, Greffe Y, Charpentier E, Martiny L Our previous studies have shown that camptothecin (CPT) and doxorubicin (DOX) triggered ceramide accumulation via de novo synthesis pathway. De novo ceramide generation was responsible for the drug-induced apoptosis through a caspase-3-dependent pathway and a decrease of thrombospondin-1 (TSP-1) expression in human thyroid carcinoma FTC-133 cells. Here, we demonstrate that Jun N-terminal kinases (JNKs) play a critical role in CPT- and DOX-induced down-regulation of TSP-1 expression : i) de novo ceramide synthesis pathway activates JNK 1/2 resulting in ATF-2 phosphorylation, ii) cell treatment by SP600125, a JNK specific inhibitor, strongly reduced ATF-2 phosphorylation and completely abolished CPT and DOX effects and ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518193 Wed, 16 Feb 2011 00:00:00 +0100 4518193 http://www.medworm.com/index.php?rid=4518227&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21315112%26dopt%3DAbstract This study is the first to demonstrate that K(+) channels such as hEag1 are directly involved in the IGF-1-induced up-regulation of cyclin D1 and E expression in MCF-7 cells. By identifying more specifically the temporal position of the arrest site induced by the inhibition of hEag1 channels, we confirmed that hEag1 activity is predominantly upstream of the arrest site induced by serum-deprivation, prior to the up-regulation of both cyclins D1 and E. PMID: 21315112 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518227 Tue, 15 Feb 2011 00:00:00 +0100 4518227 http://www.medworm.com/index.php?rid=4518186&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21334999%26dopt%3DAbstract Authors: Namslauer I, Dietz M, Brzezinski P A number of missense mutations in subunit I of cytochromec oxidase (CytcO) have previously been linked to prostate cancer (Petros et al. (2005) PNAS, 102, 719). To investigate the effects of these mutations at the molecular level, in the present study we prepared four different structural variants of the bacterial Rhodobacter sphaeroides CytcO (cytochromeaa(3)), each carrying one amino-acid residue replacement corresponding to the following substitutions identified in the above-mentioned study: Asn11Ser, Ala122Thr, Ala341Ser and Val380Ile (residues Asn25, Ser168, Ala384 and Val423 in the R.sphaeroides oxidase). This bacterial CytcO displays essentially the same structural and functional characteristics as those of the mitochondrial counterpar... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518186 Tue, 15 Feb 2011 00:00:00 +0100 4518186 http://www.medworm.com/index.php?rid=4518195&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21329734%26dopt%3DAbstract In this report, we describe our findings demonstrating that visfatin stimulates the mammalian target of the rapamycin (mTOR) pathway, which plays important roles in angiogenesis. Visfatin induced the expression of hypoxia-inducible factor 1α (HIF1α) and vascular endothelial growth factor (VEGF) in human endothelial cells. Inhibition of the mTOR pathway by rapamycin eliminated the angiogenic and proliferative effects of visfatin. The visfatin-induced increase in VEGF expression was also eliminated by RNA interference-mediated knockdown of the 70-kDa ribosomal protein S6 kinase (p70S6K), a downstream target of mTOR. Visfatin inactivated glycogen synthase kinase 3β (GSK3β) by phosphorylating it at Ser-9, leading to the nuclear translocation of β-catenin. Both rapamycin co-treatment and p... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518195 Mon, 14 Feb 2011 00:00:00 +0100 4518195 http://www.medworm.com/index.php?rid=4518204&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320464%26dopt%3DAbstract This article is part of a Special Issue entitled: Allosteric cooperativity in respiratory protiens. PMID: 21320464 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518204 Sat, 12 Feb 2011 00:00:00 +0100 4518204 http://www.medworm.com/index.php?rid=4518203&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320465%26dopt%3DAbstract Authors: Rosca M, Minkler P, Hoppel CL Mitochondrial dysfunction is a major contributor in heart failure (HF). We investigated whether the decrease in respirasome organization reported by us previously in cardiac mitochondria in HF is due to changes in the phospholipids of the mitochondrial inner membrane or modifications of the subunits of the electron transport chain (ETC) complexes. The contents of the main phospholipid species, including cardiolipin, as well as the molecular species of cardiolipin were unchanged in cardiac mitochondria in HF. Oxidized cardiolipin molecular species were not observed. In heart mitochondria isolated from HF, complex IV not incorporated into respirasomes exhibits increased threonine phosphorylation. Since HF is associated with increased adrenergic driv... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518203 Sat, 12 Feb 2011 00:00:00 +0100 4518203 http://www.medworm.com/index.php?rid=4518202&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320534%26dopt%3DAbstract Authors: Camperchioli A, Mariani M, Bartollino S, Petrella L, Persico M, Orteca N, Scambia G, Shahabi S, Ferlini C, Fattorusso C Bcl-2 plays a prominent role in regulating the function of mitochondria during respiration and in determining the threshold of apoptotic sensitivity. Despite its relevance, the mechanism through which these processes are achieved is still unknown. Using surface plasmon resonance technology to monitor Bcl-2 multimerisation we discovered that a simple dimeric model does not fit with experimental data. A molecular model of the experimentally observed Bcl2 homomeric complex has been developed. Accordingly, using a panel of mutants we identified in the loop a critical region for the process of Bcl-2 multimerisation. Our results indicate that the Bcl-2 loop posttra... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518202 Sat, 12 Feb 2011 00:00:00 +0100 4518202 http://www.medworm.com/index.php?rid=4518201&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320536%26dopt%3DAbstract Authors: Fritz RD, Radziwill G FoxO transcription factors mediate anti-proliferative and pro-apoptotic signals and act as tumor suppressors in cancer. Posttranslational modifications including phosphorylation and acetylation regulate FoxO activity by a cytoplasmic-nuclear shuttle mechanism. Scaffold proteins coordinating signaling pathways in time and space play a critical role in this process. CNK1 acts as a scaffold protein in several signaling pathways controlling the function of FoxO proteins. An understanding of CNK1 and other scaffolds in the FoxO signaling network will provide insights how to release the tumor suppressor function of FoxO as a possibility to block oncogenic pathways. PMID: 21320536 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518201 Sat, 12 Feb 2011 00:00:00 +0100 4518201 http://www.medworm.com/index.php?rid=4518200&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320572%26dopt%3DAbstract CONCLUSIONS: Comparative genomic approaches, pioneered in budding yeasts, have resulted in dramatic improvements in our understanding of the function of both genes and regulatory sequences. Analogous studies in other systems, including humans, demonstrate the ubiquity of comparative genomic approaches. Recently, forward genomic approaches, exploiting natural variation within yeast populations, have started to offer powerful insights into how genotype influences phenotype and even the ability to predict phenotypes. GENERAL SIGNIFICANCE: Comparative genomic experiments are defining the fundamental rules that govern complex traits in natural populations from yeast to humans. This article is part of a Special Issue entitled Systems Biology of Microorganisms. PMID: 21320572 [PubMed - as sup... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518200 Sat, 12 Feb 2011 00:00:00 +0100 4518200 http://www.medworm.com/index.php?rid=4518197&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320597%26dopt%3DAbstract Authors: Chung S, Son GH, Kim K Glucocorticoid (GC) is an adrenal steroid hormone that controls a variety of physiological processes such as metabolism, immune response, cardiovascular activity, and brain function. In addition to GC induction in response to stress, even in relatively undisturbed states its circulating level is subjected to a robust daily variation with a peak around the onset of the active period of the day. It has long been believed that the synthesis and secretion of GC are primarily regulated by the hypothalamus-pituitary-adrenal (HPA) neuroendocrine axis. However, recent chronobiological research strongly supports the idea that multiple regulatory mechanisms along with the classical HPA neuroendocrine axis underlie the diurnal rhythm of circulating GC. Most notably... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518197 Sat, 12 Feb 2011 00:00:00 +0100 4518197 http://www.medworm.com/index.php?rid=4518196&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320632%26dopt%3DAbstract Authors: de Pas NC, Woutersen RA, van Ommen B, Rietjens IM, de Graaf AA The LDL cholesterol (LDL-C) and HDL cholesterol (HDL-C) concentrations are determined by the activity of a complex network of reactions in several organs. Physiologically-based kinetic (PBK) computational models can be used to describe these different reactions in an integrated, quantitative manner. A PBK model to predict plasma cholesterol levels in the mouse was developed, validated, and analyzed. Kinetic parameters required for defining the model were obtained using data from published experiments. To construct the model, a set of appropriate submodels was selected from a set of 65,536 submodels differing in the kinetic expressions of the reactions. A submodel was considered appropriate if it had the ability to ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4518196 Sat, 12 Feb 2011 00:00:00 +0100 4518196 http://www.medworm.com/index.php?rid=4460543&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F01%252F25%252009.15%2522%255BMHDA%255D%253A%25222011%252F02%252F11%252007.45%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F01%252F25%252009.15%2522%255BEDAT%255D%29%29 120 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/02/11PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4460543 Fri, 11 Feb 2011 12:45:03 +0100 4460543 http://www.medworm.com/index.php?rid=4394176&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222011%252F01%252F06%252011.47%2522%255BMHDA%255D%253A%25222011%252F01%252F25%252009.15%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222011%252F01%252F06%252011.47%2522%255BEDAT%255D%29%29 178 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/01/25PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4394176 Tue, 25 Jan 2011 14:15:02 +0100 4394176 http://www.medworm.com/index.php?rid=4314875&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsites%2Fentrez%3Fcmd%3DSearch%26db%3Dpubmed%26term%3D%28%28%28%2522Biochim%2520Biophys%2520Acta%2522%29%2520AND%2520%25222010%252F12%252F20%252005.40%2522%255BMHDA%255D%253A%25222011%252F01%252F06%252011.47%2522%255BMHDA%255D%29%29%2520NOT%2520%28%28%2520%28%28%2522Biochim%2520Biophys%2520Acta%2522%255BTIAB%255D%29%29%2520AND%2520%25220001%2522%255BEDAT%255D%253A%25222010%252F12%252F20%252005.40%2522%255BEDAT%255D%29%29 130 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochim Biophys Acta" These pubmed results were generated on 2011/01/06PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4314875 Thu, 06 Jan 2011 16:47:02 +0100 4314875 http://www.medworm.com/index.php?rid=4215003&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21112469%26dopt%3DAbstract Authors: Krag SS PMID: 21112469 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4215003 Wed, 01 Dec 2010 11:25:14 +0100 4215003 http://www.medworm.com/index.php?rid=4182769&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21073998%26dopt%3DAbstract Authors: Macmillan F, Hunte C PMID: 21073998 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4182769 Fri, 19 Nov 2010 19:35:16 +0100 4182769 http://www.medworm.com/index.php?rid=4135855&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21035037%26dopt%3DAbstract Authors: Schoner W PMID: 21035037 [PubMed - in process] (Source: Biochimica et Biophysica Acta) Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135855 Fri, 05 Nov 2010 19:20:58 +0100 4135855 http://www.medworm.com/index.php?rid=4135842&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21047566%26dopt%3DAbstract Authors: Akama K, Horikoshi T, Nakayama T, Otsu M, Imaizumi N, Nakamura M, Toda T, Inuma M, Hirano H, Kondo Y, Suzuki Y, Inoue N Understanding neurogenesis is valuable for the treatment of nervous system disorders. However, there is currently limited information about the molecular events associated with the transition from primate ES cells to neural cells. We therefore sought to identify the proteins involved in neurogenesis, from Macaca fascicularis ES cells (CMK6 cell line) to neural stem (NS) cells to neurons using two-dimensional gel electrophoresis (2-DE), peptide mass fingerprinting (PMF), and liquid chromatography-tandem mass spectrometry (LC-MS-MS). During the differentiation of highly homogeneous ES cells to NS cells, we identified 17 proteins with increased expression, inclu... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135842 Sun, 31 Oct 2010 00:00:00 +0100 4135842 http://www.medworm.com/index.php?rid=4135848&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21040698%26dopt%3DAbstract Authors: Preté PS, Domingues CC, Meirelles NC, Malheiros SV, Goñi FM, de Paula E, Schreier S The various stages of the interaction between the detergent Triton X-100 (TTX-100) and membranes of whole red blood cells (RBC) were investigated in a broad range of detergent concentrations. The interaction was monitored by RBC hemolysis - assessed by release of intracellular hemoglobin (Hb) and inorganic phosphate - and by analysis of EPR spectra of a fatty acid spin probe intercalated in whole RBC suspensions, as well as pellets and supernatants obtained upon centrifugation of detergent-treated cells. Hemolysis finished at ca. 0.9mM TTX-100. Spectral analysis and calculation of order parameters (S) indicated that a complex sequence of events takes place, and allowed the characterization of... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135848 Fri, 29 Oct 2010 00:00:00 +0100 4135848 http://www.medworm.com/index.php?rid=4135847&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21040746%26dopt%3DAbstract Authors: Baxter SA, Cheung DY, Bocangel P, Kim HK, Herbert K, Douville JM, Jangamreddy JR, Zhang S, Eisenstat DD, Wigle JT The homeobox transcription factor PROX1 is essential for the development and maintenance of lymphatic vasculature. How PROX1 regulates lymphatic endothelial cell fate remains undefined. PROX1 has been shown to upregulate the expression of Cyclin E, which mediates the G(1) to S transition of the cell cycle. Here we demonstrate that PROX1 activates the mouse Cyclin E1 (Ccne1) promoter via two proximal E2F binding sites. We have determined that the N-terminal region of PROX1 is sufficient to activate a 1kb Ccne1 promoter whereas the homeodomain is dispensable for activation. We have identified that the Prospero domain 1 (PD1) is required for the nuclear localization o... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135847 Fri, 29 Oct 2010 00:00:00 +0100 4135847 http://www.medworm.com/index.php?rid=4135846&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21040802%26dopt%3DAbstract In this study we have investigated the molecular requirements underlying the stimulatory effect of LpE on axonal extension. We show that enhancement of axonal growth by LpE requires the presence of the low density lipoprotein receptor-related protein-1 (LRP1) in neurons since RNA silencing of LRP1 in neurons, or antibodies directed against LRP, suppressed the LpE-induced axonal extension. In contrast, an alternative LRP1 ligand, α2-macroglobulin, failed to stimulate axonal extension suggesting that LpE do not exert their growth-stimulatory effect solely by activation of a LRP1-mediated signaling pathway. In addition, although apoE3-containing LpE enhanced axonal extension, apoE4-containing LpE did not. Over-expression of ABCG1 in rat cortical glial cells resulted in production of LpE that... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135846 Fri, 29 Oct 2010 00:00:00 +0100 4135846 http://www.medworm.com/index.php?rid=4135845&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21040803%26dopt%3DAbstract Authors: Tanaka M, Dhanasekaran P, Nguyen D, Nickel M, Takechi Y, Lund-Katz S, Phillips MC, Saito H As the principal component of high-density lipoprotein (HDL), apolipoprotein (apo) A-I plays essential roles in lipid transport and metabolism. Because of its intrinsic conformational plasticity and flexibility, the molecular details of the tertiary structure of lipid-free apoA-I have not been fully elucidated. Previously, we demonstrated that the stability of the N-terminal helix bundle structure is modulated by proline substitution at the most hydrophobic region (residues around Y18) in the N-terminal domain. Here we examine the effect of proline substitution at S55 located in another relatively hydrophobic region compared to most of the helix bundle domain to elucidate the influences ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135845 Fri, 29 Oct 2010 00:00:00 +0100 4135845 http://www.medworm.com/index.php?rid=4135854&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21036139%26dopt%3DAbstract Authors: Sedoud A, Kastner L, Cox N, El-Alaoui S, Kirilovsky D, Rutherford AW EPR was used to study the influence of formate on the electron acceptor side of photosystem II (PSII) from Thermosynechococcus elongatus. Two new EPR signals were found and characterized. The first is assigned to the semiquinone form of Q(B) interacting magnetically with a high spin, non-heme-iron (Fe(2+), S = 2) when the native bicarbonate/carbonate ligand is replaced by formate. This assignment is based on several experimental observations, the most important of which were: i) its presence in the dark in a significant fraction of centers, and ii) the period-of-two variations in the concentration expected for Q(B)(•-) when PSII underwent a series of single-electron turnovers. This signal is similar but not... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135854 Thu, 28 Oct 2010 00:00:00 +0100 4135854 http://www.medworm.com/index.php?rid=4135853&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21036140%26dopt%3DAbstract Authors: Stadnichuk IN, Bulychev AA, Lukashev EP, Sinetova MP, Khristin MS, Johnson MP, Ruban AV Phycobilisomes (PBS) are the major photosynthetic antenna complexes in cyanobacteria and red algae. In the red microalga Galdieria sulphuraria, action spectra measured separately for photosynthetic activities of photosystem I (PSI) and photosystem II (PSII) demonstrate that PBS-fraction attributed to PSI is more sensitive to stress conditions and upon nitrogen starvation disappears from the cell earlier than the fraction of PBS coupled to PSII. Pre-illumination of the cells by actinic far-red light primarily absorbed by PSI caused an increase in the amplitude of the PBS-low-temperature fluorescence emission that was accompanied by the decrease in PBS region of the PSI 77K fluorescence excit... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135853 Thu, 28 Oct 2010 00:00:00 +0100 4135853 http://www.medworm.com/index.php?rid=4135852&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21036141%26dopt%3DAbstract Authors: Busch S, Unruh T The influence of additives on the molecular dynamics of the phospholipid dimyristoylphosphatidylcholine (DMPC) in its fully hydrated liquid crystalline phase was studied. Quasielastic neutron scattering (QENS) was used to detect motions with dimensions of some Ångstroms on two different time scales, namely 60ps and 900ps. The effects of myristic acid, farnesol, cholesterol, and sodium glycocholate could consistently be explained on the basis of collective, flow-like motions of the phospholipid molecules. The influence of the additives on these motions was explained by packing effects, corresponding the reduction of free volume. Cholesterol was found to decrease the mobility of DMPC seen on the 900ps time scale with increasing cholesterol content. In contrast,... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135852 Thu, 28 Oct 2010 00:00:00 +0100 4135852 http://www.medworm.com/index.php?rid=4135851&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21036142%26dopt%3DAbstract In this study, we investigated the role of the omega-3 fatty acid docosahexaenoic acid (DHA) in modulation of membrane fluidity, APP processing and protection from cytotoxic stress. To this end, we applied unilamellar transfer liposomes which provided protection from oxidation and effective incorporation of DHA into cell membranes. Liposomes transferring docosanoic acid (DA), the completely saturated form of DHA, to the cell cultures served as controls. In HEK-APP cells, DHA significantly increased membrane fluidity and non-amyloidogenic processing of APP, leading to enhanced secretion of sAPPα. This enhanced secretion of sAPPα was associated with substantial protection against apoptosis induced by ER Ca(2+) store depletion. sAPPα-containing supernatants obtained from HEK-APP cells exer... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135851 Thu, 28 Oct 2010 00:00:00 +0100 4135851 http://www.medworm.com/index.php?rid=4135850&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21036143%26dopt%3DAbstract Authors: Groen D, Poole DS, Gooris GS, Bouwstra JA The lipid organization in the stratum corneum (SC), plays an important role in the barrier function of the skin. SC lipids form two lamellar phases with a predominantly orthorhombic packing. In previous publications a lipid model was presented, referred to as the stratum corneum substitute (SCS), that closely mimics the SC lipid organization and barrier function. Therefore, the SCS serves as a unique tool to relate lipid organization with barrier function. In the present study we examined the effect of the orthorhombic to hexagonal phase transition on the barrier function of human SC and SCS. In addition, the SCS was modified by changing the free fatty acid composition, resulting in a hexagonal packing and perturbed lamellar organizati... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135850 Thu, 28 Oct 2010 00:00:00 +0100 4135850 http://www.medworm.com/index.php?rid=4135849&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21036202%26dopt%3DAbstract Authors: Okoh V, Deoraj A, Roy D Elevated lifetime estrogen exposure is a major risk factor for breast cancer. Recent advances in the understanding of breast carcinogenesis clearly indicate that induction of E(2)/estrogen receptor (ER) mediated signaling is not sufficient for the development of breast cancer. The underlying mechanisms of breast susceptibility to estrogen's carcinogenic effect remain elusive. Physiologically achievable concentrations of estrogen or estrogen metabolites however, directly acting on mitochondria of mammary epithelial cells has been shown to generate reactive oxygen species (ROS). Recent data implicated these ROS into estrogen-induced DNA synthesis, increased phosphorylation of kinases, and activation of transcription factors, e.g., AP-1, NRF1, E2F, NFkB an... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135849 Thu, 28 Oct 2010 00:00:00 +0100 4135849 http://www.medworm.com/index.php?rid=4135844&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21044883%26dopt%3DAbstract Authors: Fleisch VC, Fraser B, Allison WT Zebrafish possess a robust, innate CNS regenerative ability. Combined with their genetic tractability and vertebrate CNS architecture, this ability makes zebrafish an attractive model to gain requisite knowledge for clinical CNS regeneration. In treatment of neurological disorders, one can envisage replacing lost neurons through stem cell therapy or through activation of latent stem cells in the CNS. Here we review the evidence that radial glia are a major source of CNS stem cells in zebrafish and thus activation of radial glia is an attractive therapeutic target. We discuss the regenerative potential and the molecular mechanisms thereof, in the zebrafish spinal cord, retina, optic nerve and higher brain centres. We evaluate various cell ablati... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135844 Thu, 28 Oct 2010 00:00:00 +0100 4135844 http://www.medworm.com/index.php?rid=4135843&cid=s_34400_60_f&fid=34400&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21044894%26dopt%3DAbstract Authors: Connelly PW, Picardo CM, Potter PM, Teiber JF, Maguire GF, Ng DS Recent studies suggest that paraoxonase-1 (PON1), complexed with high density lipoproteins, is the major lactonase in the circulation. Using 5-hydroxy eicosatetraenoate δ-lactone (5-HETEL) as the substrate, we observed lactonase activity in serum from Pon1-/- mice. However, 6-12 carbon fatty acid γ and δ lactones were not hydrolyzed in serum from Pon1-/- mice. Serum from both wild type and Pon1-/- mice contained a lactonase activity towards 5-HETEL and 3-oxo-dodecanoyl-homoserine lactone that was resistant to inactivation by EDTA. This lactonase activity was sensitive to the serine esterase inhibitor phenyl methyl sulfonyl fluoride and co-eluted with carboxylesterase activity by size-exclusion chromatography. ... Biochimica et Biophysica Acta journals http://www.medworm.com/rss/comments.php?id=4135843 Thu, 28 Oct 2010 00:00:00 +0100 4135843