MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Biology of the Cell' source. Tue, 07 Feb 2012 08:48:51 +0100 http://www.medworm.com/index.php?rid=5659176&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296452%26dopt%3DAbstract Authors: PMID: 22296452 [PubMed - in process] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5659176 Wed, 01 Feb 2012 05:00:00 +0100 5659176 http://www.medworm.com/index.php?rid=5659175&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296453%26dopt%3DAbstract Authors: PMID: 22296453 [PubMed - in process] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5659175 Wed, 01 Feb 2012 05:00:00 +0100 5659175 http://www.medworm.com/index.php?rid=5659177&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22289110%26dopt%3DAbstract CONCLUSIONS: Expressing β5-GFP is sufficient to reverse phagocytic deficiencies of RPE cells derived from β5(-/-) mice indicating that these cells do not irreversibly lose other components of the phagocytic machinery. RPE cells expressing the engulfment receptor MerTK control POS binding by limiting activity of endogenous αvβ5 and αvβ5-GFP integrins although they reside at the apical, phagocytic surface. In contrast, RPE cells permanently or transiently losing MerTK expression lack this regulatory mechanism and bind excess POS via surface αvβ5 receptors. Taken together, these data reveal a novel feedback mechanism that restricts binding of POS to surface avb5 integrin receptors in RPE cells. PMID: 22289110 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5659177 Mon, 30 Jan 2012 05:00:00 +0100 5659177 http://www.medworm.com/index.php?rid=5659178&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22283414%26dopt%3DAbstract Authors: Li S, Yang C, Hong Y, Bi H, Zhao F, Liu Y, Ao X, Pang P, Xing X, Chang AK, Xiao L, Zhang Y, Wu H Abstract Amplified in breast cancer 1 (AIB1) is a transcriptional coactivator of nuclear receptors and other transcription factors. It is required for animal growth and reproductive development, and has also been implicated in breast carcinogenesis. Although AIB1 is known to be covalently modified by SUMO-1, which serves to regulate its stability and transcriptional activity, the exact SUMO E3 ligase involved in its sumoylation has not been determined. Here we show that one member of the PIAS family, PIAS1, co-immunoprecipitated with AIB1 in extract prepared from breast cancer cells (MCF-7), and that over-expression of PIAS1 together with AIB1 in MCF-7 cells led to increased su... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5659178 Fri, 27 Jan 2012 05:00:00 +0100 5659178 http://www.medworm.com/index.php?rid=5659179&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22276751%26dopt%3DAbstract Authors: Sperry AO Abstract Mammalian spermatogenesis is characterized by dramatic cellular change to transform the non-polar spermatogonium into the highly polarized, functional spermatozoon. The acquisition of cell polarity is a requisite step for formation of viable sperm. The polarity of the spermatozoon is clearly demonstrated by the acrosome at the apical pole of the cell and the flagellum at the opposite end. Spermatogenesis consists of three basic phases: mitosis, meiosis, and spermiogenesis. The final phase represents the period of greatest cellular change where cell-type specific organelles such as the acrosome and the flagellum form, the nucleus migrates to the plasma membrane and elongates, chromatin condenses, and residual cytoplasm is removed. An important feature of ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5659179 Thu, 26 Jan 2012 05:00:00 +0100 5659179 http://www.medworm.com/index.php?rid=5659180&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22268789%26dopt%3DAbstract Authors: Jäger R, Bertrand MJ, Gorman AM, Vandenabeele P, Samali A Abstract One of the early cellular responses to endoplasmic reticulum (ER) stress is the activation of the unfolded protein response (UPR). ER stress and the UPR are both implicated in numerous human diseases and pathologies. In spite of this, our knowledge of the molecular mechanisms that regulate cell fate following ER stress is limited. The UPR is initiated by three ER transmembrane receptors, PERK, ATF6, and IRE1. These proteins sense the accumulation of unfolded proteins and their activation triggers specific adaptive responses to resolve the stress. Intriguingly, the very same receptors can initiate signaling pathways that lead to apoptosis when the attempts to resolve the ER stress fail. In this review we de... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5659180 Mon, 23 Jan 2012 05:00:00 +0100 5659180 http://www.medworm.com/index.php?rid=5623478&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22251092%26dopt%3DAbstract Authors: Marvin SA, Wiethoff CM Abstract Adenovirus relies on numerous interactions between viral and host cell proteins to efficiently enter cells. Undoubtedly, post-translational modifications of host and cellular proteins can impact the efficiency of this cell entry process. Ubiquitylation, once simply thought of as a modification targeting proteins for proteasomal degradation, is now known to regulate protein trafficking within cells, protein-protein interactions, and cell signaling pathways. Accumulating evidence suggests that protein ubiquitylation can influence all stages of the life cycle of other viruses such as cell entry, replication, and egress. Until recently the influence of ubiquitylation has only been documented during adenovirus replication. This review highlights ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5623478 Tue, 17 Jan 2012 05:00:00 +0100 5623478 http://www.medworm.com/index.php?rid=5560891&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22211456%26dopt%3DAbstract Authors: Carmosino M, Procino G, Svelto M Abstract The central role of Na+-K+-2Cl- cotrasporter type 2 (NKCC2) in the vectorial transepithelial salt reabsorption in thick ascending limb cells from Henle's loop in the kidney is evidenced by the effects of loop diuretics, the pharmacological inhibitors of NKCC2, that are among the most powerful antihypertensive drugs available to date. Moreover, genetic mutations of the NKCC2 encoding gene resulting in impaired apical targeting and function of NKCC2 transporter, give rice to a pathological phenotype known as Type I Bartter's syndrome, characterized by a severe volume depletion, hypokalemia and metabolic alkalosis with high prenatal mortality. On the other hand, excessive NKCC2 activity has been linked with inherited hypertension in h... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5560891 Mon, 02 Jan 2012 05:00:00 +0100 5560891 http://www.medworm.com/index.php?rid=5560890&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22211516%26dopt%3DAbstract CONCLUSIONS: Our results show that MT depolymerization is particularly disruptive when cells interact with pliable 3D matrices, suggesting a role for microtubules and the Rho pathway in the fine-tuning of contractile and adhesive forces necessary to sustain cell motility in vivo. PMID: 22211516 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5560890 Mon, 02 Jan 2012 05:00:00 +0100 5560890 http://www.medworm.com/index.php?rid=5560889&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22214190%26dopt%3DAbstract Authors: PMID: 22214190 [PubMed - in process] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5560889 Sun, 01 Jan 2012 05:00:00 +0100 5560889 http://www.medworm.com/index.php?rid=5560888&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22214191%26dopt%3DAbstract Authors: PMID: 22214191 [PubMed - in process] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5560888 Sun, 01 Jan 2012 05:00:00 +0100 5560888 http://www.medworm.com/index.php?rid=5535562&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187938%26dopt%3DAbstract CONCLUSIONS: Thus, these studies have evaluated mechanistic roles for dynamin-dependant, clathrin-mediated and caveolar endocytosis during epithelial wound healing, and have provided contrasting observations between analyses of cell motility in fibroblast models and epithelial cells. In conclusion these studies have identified a novel mechanism for regulation of occludin during wound healing. PMID: 22187938 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5535562 Mon, 19 Dec 2011 05:00:00 +0100 5535562 http://www.medworm.com/index.php?rid=5535561&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22187967%26dopt%3DAbstract CONCLUSIONS: SD-dSTORM presents a significant advance towards user-friendly single molecule localization-based super-resolution microscopy combining advantages of state-of-the-art methodologies to perform fast, reliable and efficient multi-color dSTORM. PMID: 22187967 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5535561 Mon, 19 Dec 2011 05:00:00 +0100 5535561 http://www.medworm.com/index.php?rid=5535555&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22188301%26dopt%3DAbstract Authors: Biard-Piechaczyk M, Borel S, Espert L, de Bettignies G, Coux O Abstract The modification of intracellular proteins by Ubiquitin and Ubiquitin-like proteins is a central mechanism for regulating and fine-tuning all cellular processes. Indeed, these modifications are widely used to control the stability, activity and localization of many key proteins and therefore they are instrumental in regulating cellular functions as diverse as protein degradation, cell signalling, vesicle trafficking and immune response. It is thus no surprise that pathogens in general, and viruses in particular, have developed multiple strategies to either counteract or exploit the complex mechanisms mediated by the Ubiquitin and Ubiquitin-like protein conjugation pathways. The aim of this review is to... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5535555 Mon, 19 Dec 2011 05:00:00 +0100 5535555 http://www.medworm.com/index.php?rid=5535560&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22188018%26dopt%3DAbstract This report shows for the first time that Rab11 is differentially phosphorylated by distinct PKC isoenzymes and that this post-translational modification might be a regulatory mechanism of intracellular trafficking. PMID: 22188018 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5535560 Wed, 07 Dec 2011 05:00:00 +0100 5535560 http://www.medworm.com/index.php?rid=5535559&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22188068%26dopt%3DAbstract Authors: Van Opdenbosch N, Favoreel H, Van de Walle GR Abstract In eukaryotic cells, gene expression is not only regulated by transcription factors but also by several epigenetic mechanisms including post-translational modifications of histone proteins. There are numerous histone modifications described to date and methylation, acetylation, ubiquitination and phosphorylation are amongst the best studied. In parallel, certain viruses interact with the very same regulatory mechanisms, hereby manipulating the normal epigenetic landscape of the host cell, to fit their own replication needs. This review concentrates on herpesviruses specifically and how they interfere with the histone-modifying enzymes to regulate their replication cycles. Herpesviruses vary greatly with respect to the ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5535559 Wed, 07 Dec 2011 05:00:00 +0100 5535559 http://www.medworm.com/index.php?rid=5535558&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22188132%26dopt%3DAbstract CONCLUSIONS: Given that sustained ATP release from glia upon injury greatly contribute to secondary brain damage and cathepsin B plays a critical role in glioma dissemination, TI-VAMP silencing can represent a novel strategy to control lysosome fusion in pathological conditions. PMID: 22188132 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5535558 Wed, 07 Dec 2011 05:00:00 +0100 5535558 http://www.medworm.com/index.php?rid=5535557&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22188167%26dopt%3DAbstract CONCLUSIONS: Taken together, these data illustrate that Rab30 primarily localises to the Golgi apparatus and is required for the structural integrity of this organelle. PMID: 22188167 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5535557 Mon, 05 Dec 2011 05:00:00 +0100 5535557 http://www.medworm.com/index.php?rid=5535556&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22188262%26dopt%3DAbstract Authors: Wang YE, Pernet O, Lee B Abstract The nucleocytoplasmic trafficking of many cellular proteins is regulated by nuclear import/export signals as well as post-translational modifications such as the covalent conjugation of ubiquitin and the small ubiquitin-related modifiers (SUMOs). Ubiquitination and SUMOylation are rapid and reversible ways to modulate the intracellular localization and function of the substrate proteins. These pathways have been co-opted by some viruses, which depend on the host cell machinery to transport their proteins in and out of the nucleus. In this review, we will summarize our current knowledge on the ubiquitin/SUMO-regulated nuclear/subnuclear trafficking of cellular proteins and describe examples of viral exploitation of these pathways. PMID:... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5535556 Mon, 05 Dec 2011 05:00:00 +0100 5535556 http://www.medworm.com/index.php?rid=5421933&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22054418%26dopt%3DAbstract Authors: Willier S, Butt E, Richter GH, Burdach S, Grunewald TG Abstract Integrating signals from the ECM (extracellular matrix) via the cell surface into the nucleus is an essential feature of multicellular life and often malfunctions in cancer. To date many signal transducers known as shuttle proteins have been identified that act as both: a cytoskeletal and a signalling protein. Here, we highlight the interesting member of the Zyxin family TRIP6 [thyroid receptor interactor protein 6; also designated ZRP-1 (zyxin-related protein 1)] and review current literature to define its role in cell physiology and cancer. TRIP6 is a versatile scaffolding protein at FAs (focal adhesions) involved in cytoskeletal organization, coordinated cell migration and tissue invasion. Via its LIM and T... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5421933 Sat, 19 Nov 2011 02:42:21 +0100 5421933 http://www.medworm.com/index.php?rid=5421932&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22054419%26dopt%3DAbstract Authors: Le Guelte A, Dwyer J, Gavard J Abstract The endothelial barrier controls the passage of fluids, nutrients and cells through the vascular wall. This physiological function is closely related to developmental and adult angiogenesis, blood pressure control, as well as immune responses. Moreover, cancer progression is frequently characterized by disorganized and leaky blood vessels. In this context, vascular permeability drives tumour-induced angiogenesis, blood flow disturbances, inflammatory cell infiltration and tumour cell extravasation. Although various molecules have been implicated, the vascular endothelial adhesion molecule, VE-cadherin (vascular endothelial cadherin), has emerged as a critical player involved in maintaining endothelial barrier integrity and homoeostas... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5421932 Sat, 19 Nov 2011 02:42:11 +0100 5421932 http://www.medworm.com/index.php?rid=5219299&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21899517%26dopt%3DAbstract Conclusions: These data indicate that the unusual organisation of individual Golgi stacks dispersed throughout the cytoplasm of these terminally differentiated cells is likely to be a developmentally regulated event. PMID: 21899517 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5219299 Thu, 08 Sep 2011 04:00:00 +0100 5219299 http://www.medworm.com/index.php?rid=5205539&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20110074 Conclusions: These data indicate that the unusual organisation of individual Golgi stacks dispersed throughout the cytoplasm of these terminally differentiated cells is likely to be a developmentally regulated event. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5205539 Thu, 08 Sep 2011 04:00:00 +0100 5205539 http://www.medworm.com/index.php?rid=5104198&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21812762%26dopt%3DAbstract Authors: Hurbain I, Sachse M Our knowledge of the organization of the cell is linked, to a great extent, to light and electron microscopy. Choosing either photons or electrons for imaging has many consequences on the image obtained, as well as on the experiment required in order to generate the image. One apparent effect on the experimental side is in the sample preparation, which can be quite elaborate for electron microscopy. In recent years, rapid freezing, cryo-preparation and cryo-electron microscopy have been more widely used because they introduce fewer artefacts during preparation when compared with chemical fixation and room temperature processing. In addition, cryo-electron microscopy allows the visualization of the hydrated specimens. In the present review, we give an introd... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5104198 Mon, 08 Aug 2011 00:00:11 +0100 5104198 http://www.medworm.com/index.php?rid=5095583&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20110049 Conclusions: Our results endorse the use of these cellular models for the in-depth study of the currently poorly understood functions of SSAO/VAP-1, its involvement in the above-mentioned pathologies, as well as for evaluating potential compounds that could modulate its activity for therapeutic purposes. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5095583 Thu, 04 Aug 2011 23:00:00 +0100 5095583 http://www.medworm.com/index.php?rid=5104199&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21810080%26dopt%3DAbstract Conclusions. Our procedure could be used for developing new research protocols in the prospect of using these cells as therapeutic agents. PMID: 21810080 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5104199 Mon, 01 Aug 2011 23:00:00 +0100 5104199 http://www.medworm.com/index.php?rid=5095584&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100112 Conclusions. Our procedure could be used for developing new research protocols in the prospect of using these cells as therapeutic agents. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5095584 Mon, 01 Aug 2011 23:00:00 +0100 5095584 http://www.medworm.com/index.php?rid=5069213&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20110024 Conclusions. We report for the first time the protein endowment of Caco-2/TC7 enterocyte CLD. Our results suggest that their formation and mobilization may participate to the control of enterocyte TRL secretion in a cell-specific manner. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5069213 Mon, 25 Jul 2011 23:00:00 +0100 5069213 http://www.medworm.com/index.php?rid=5069212&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100147 Conclusions: The increase in cell migration observed upon convertases inhibition appears to be due to the up-activation of beta1 integrins and to their location in larger focal adhesion structures. The endoproteolytic cleavage of av subunits is necessary for alphavbeta5/beta6 integrin to control alpha2beta1 function and could thus play an essential role in colon cancer cell migration (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5069212 Mon, 25 Jul 2011 23:00:00 +0100 5069212 http://www.medworm.com/index.php?rid=5050100&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20110032 Conclusion. These data demonstrate that pes1 and ppan are required for Xenopus pronephros development and indicate that their function in the pronephros is independent of their role in ribosome biosynthesis. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5050100 Mon, 18 Jul 2011 23:00:00 +0100 5050100 http://www.medworm.com/index.php?rid=5009199&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21736554%26dopt%3DAbstract Authors: Shen WW, Frieden M, Demaurex N SOCE (store-operated calcium entry) is a ubiquitous cellular mechanism linking the calcium depletion of the ER (endoplasmic reticulum) to the activation of PM (plasma membrane) Ca2+-permeable channels. The activation of SOCE channels favours the entry of extracellular Ca2+ into the cytosol, thereby promoting the refilling of the depleted ER Ca2+ stores as well as the generation of long-lasting calcium signals. The molecules that govern SOCE activation comprise ER Ca2+ sensors [STIM1 (stromal interaction molecule 1) and STIM2], PM Ca2+-permeable channels {Orai and TRPC [TRP (transient receptor potential) canonical]} and regulatory Ca2+-sensitive cytosolic proteins {CRACR2 [CRAC (Ca2+ release-activated Ca2+ current) regulator 2]}. Upon Ca2+ depleti... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5009199 Sat, 09 Jul 2011 13:30:20 +0100 5009199 http://www.medworm.com/index.php?rid=5009198&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21736555%26dopt%3DAbstract Authors: Verrier L, Vandromme M, Trouche D The 'histone code' hypothesis states that chromatin-based regulation of nuclear processes such as transcription is brought about by the combination of distinct modifications (histone marks) at specific loci. Its correct establishment involves chromatin cross-talks, ensuring an ordered and concerted deposition/removal of a particular set of modifications that act together to give the correct transcriptional outcome. Histone methylation on lysine residues can negatively or positively impact on gene transcription, depending on the residue and on its degree of methylation. Thanks to this complexity and given the number of chromatin 'readers' that can recognize methylated lysine residues, histone methylation plays a very special role in specifying ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5009198 Sat, 09 Jul 2011 13:30:15 +0100 5009198 http://www.medworm.com/index.php?rid=5009197&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21736556%26dopt%3DAbstract Authors: Alt E, Yan Y, Gehmert S, Song YH, Altman A, Gehmert S, Vykoukal D, Bai X PMID: 21736556 [PubMed - in process] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5009197 Sat, 09 Jul 2011 13:30:11 +0100 5009197 http://www.medworm.com/index.php?rid=5009201&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21732910%26dopt%3DAbstract Conclusions. Our data conclusively provide evidence for the participation of IFT proteins in photoreceptor cell ciliogenesis, including the formation of the ciliary vesicle and the elongation of the primary cilium. In advanced stages of ciliogenesis the ciliary localization of IFT proteins indicate a role in IFT as is seen in mature cilia. A prominent accumulation of IFT proteins in the periciliary cytoplasm at the base of the cilia in these stages most probably resembles a reserve pool of IFT molecules for further delivery into the growing ciliary shaft and their subsequent function in IFT. Nevertheless, the cytoplasmic localization of IFT proteins in the absence of a ciliary shaft in early stages of ciliogenesis may indicate roles of IFT proteins beyond their well-established function fo... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5009201 Tue, 05 Jul 2011 23:00:00 +0100 5009201 http://www.medworm.com/index.php?rid=5009200&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21732911%26dopt%3DAbstract Conclusions. HMEC-based lines retain most of the angiogenic features of primary endothelial cells, yet possess long-term stability and ease of culture, making them intriguing candidates for large-scale primary HC and HT screening (of ~10,000-1,000,000 molecules). Furthermore, inclusion of EMCs demonstrates the feasibility of using epicardial-derived cells, which normally contribute to smooth muscle, to model large vessel formation. In summary, the immortalized fluorescent HMEC and EMC lines and straightforward culture conditions will enable assay development for HCS of angiogenesis. PMID: 21732911 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5009200 Tue, 05 Jul 2011 23:00:00 +0100 5009200 http://www.medworm.com/index.php?rid=5007769&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20110034 Conclusions. Our data conclusively provide evidence for the participation of IFT proteins in photoreceptor cell ciliogenesis, including the formation of the ciliary vesicle and the elongation of the primary cilium. In advanced stages of ciliogenesis the ciliary localization of IFT proteins indicate a role in IFT as is seen in mature cilia. A prominent accumulation of IFT proteins in the periciliary cytoplasm at the base of the cilia in these stages most probably resembles a reserve pool of IFT molecules for further delivery into the growing ciliary shaft and their subsequent function in IFT. Nevertheless, the cytoplasmic localization of IFT proteins in the absence of a ciliary shaft in early stages of ciliogenesis may indicate roles of IFT proteins beyond their well-established function fo... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5007769 Tue, 05 Jul 2011 23:00:00 +0100 5007769 http://www.medworm.com/index.php?rid=5007768&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100146 Conclusions. HMEC-based lines retain most of the angiogenic features of primary endothelial cells, yet possess long-term stability and ease of culture, making them intriguing candidates for large-scale primary HC and HT screening (of ~10,000-1,000,000 molecules). Furthermore, inclusion of EMCs demonstrates the feasibility of using epicardial-derived cells, which normally contribute to smooth muscle, to model large vessel formation. In summary, the immortalized fluorescent HMEC and EMC lines and straightforward culture conditions will enable assay development for HCS of angiogenesis. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=5007768 Tue, 05 Jul 2011 23:00:00 +0100 5007768 http://www.medworm.com/index.php?rid=4962510&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21692748%26dopt%3DAbstract Conclusions These data suggest that the site of RanGTP generation in a mitotic somatic cell can generate critical spatial information that specifies where microtubules grow toward and where microtubules are organized. As RanGTP is normally generated on chromosomes these data suggest that RanGTP may play an important role in specifying that spindle assembly occurs around chromosomes. PMID: 21692748 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4962510 Tue, 21 Jun 2011 23:00:00 +0100 4962510 http://www.medworm.com/index.php?rid=4959843&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100135 Conclusions These data suggest that the site of RanGTP generation in a mitotic somatic cell can generate critical spatial information that specifies where microtubules grow toward and where microtubules are organized. As RanGTP is normally generated on chromosomes these data suggest that RanGTP may play an important role in specifying that spindle assembly occurs around chromosomes. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4959843 Tue, 21 Jun 2011 23:00:00 +0100 4959843 http://www.medworm.com/index.php?rid=4962511&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21679159%26dopt%3DAbstract In this study, we aimed to investigate the origins of adipose stem cells. Smooth muscle actin alpha (α-SMA) is one of the markers of pericytes. We harvested adipose stromal cells from α-SMA-GFP transgenic mice and sorted them into GFP positive and negative cells by FACS. Multilineage differentiation tests were applied to examine the pluripotent ability of the α-SMA-GFP positive and negative cells. Immunofluorescent staining for α-SMA and PDGF-Rβ were applied to identify the α-SMA-GFP positive cells. Then α-SMA-GFP positive cells were loaded on a collagen-fibronectin gel with endothelial cells to test their vascularization ability both in vitro and in vivo. Results show that in the adipose tissue, all of the α-SMA-GFP positive cells congregate around the blood vessels. Only the α-S... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4962511 Wed, 15 Jun 2011 23:00:00 +0100 4962511 http://www.medworm.com/index.php?rid=4933604&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20110033 In this study, we aimed to investigate the origins of adipose stem cells. Smooth muscle actin alpha (α-SMA) is one of the markers of pericytes. We harvested adipose stromal cells from α-SMA-GFP transgenic mice and sorted them into GFP positive and negative cells by FACS. Multilineage differentiation tests were applied to examine the pluripotent ability of the α-SMA-GFP positive and negative cells. Immunofluorescent staining for α-SMA and PDGF-Rβ were applied to identify the α-SMA-GFP positive cells. Then α-SMA-GFP positive cells were loaded on a collagen-fibronectin gel with endothelial cells to test their vascularization ability both in vitro and in vivo. Results show that in the adipose tissue, all of the α-SMA-GFP positive cells co... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4933604 Wed, 15 Jun 2011 23:00:00 +0100 4933604 http://www.medworm.com/index.php?rid=4866050&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21592090%26dopt%3DAbstract Conclusion. ROS-dependent signaling was affected by changes in temperature or oxygen availability. Feedback interactions between ROS and the glutathione redox system, possibly driven by elevated mitochondrial ROS production, likely contributed to the appearance of the ROS and GSH fluctuations upon acute environmental change. Fluctuating ROS levels, which encode for the magnitude of environmental change, could be a way to transfer information on ROS production to subsequent processes (gene expression) while avoiding too-high and damaging ROS levels. PMID: 21592090 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4866050 Wed, 18 May 2011 23:00:00 +0100 4866050 http://www.medworm.com/index.php?rid=4841102&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100145 Conclusion. ROS-dependent signaling was affected by changes in temperature or oxygen availability. Feedback interactions between ROS and the glutathione redox system, possibly driven by elevated mitochondrial ROS production, likely contributed to the appearance of the ROS and GSH fluctuations upon acute environmental change. Fluctuating ROS levels, which encode for the magnitude of environmental change, could be a way to transfer information on ROS production to subsequent processes (gene expression) while avoiding too-high and damaging ROS levels. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4841102 Wed, 18 May 2011 23:00:00 +0100 4841102 http://www.medworm.com/index.php?rid=4814456&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21554243%26dopt%3DAbstract Conclusions: Together the data suggest that sulfatide could play a role as an alternate receptor for VACV-WR and probably other Orthopoxviruses. PMID: 21554243 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4814456 Sun, 08 May 2011 23:00:00 +0100 4814456 http://www.medworm.com/index.php?rid=4802094&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20110012 Conclusions: Together the data suggest that sulfatide could play a role as an alternate receptor for VACV-WR and probably other Orthopoxviruses. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4802094 Sun, 08 May 2011 23:00:00 +0100 4802094 http://www.medworm.com/index.php?rid=4814457&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21545355%26dopt%3DAbstract Conclusions. We show that syntaxin 17 has a tyrosine based motif which is required for its incorporation into COPII vesicles, exit from the ER, and localization to the ERGIC. Our results suggest that syntaxin 17 cycles between the ER and ERGIC through classical trafficking pathways involving COPII and COPI vesicles which requires its unique C-terminal tail. We also show that syntaxin 17 is essential for maintaining the architecture of ERGIC and Golgi. PMID: 21545355 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4814457 Wed, 04 May 2011 23:00:00 +0100 4814457 http://www.medworm.com/index.php?rid=4788900&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20110006 Conclusions. We show that syntaxin 17 has a tyrosine based motif which is required for its incorporation into COPII vesicles, exit from the ER, and localization to the ERGIC. Our results suggest that syntaxin 17 cycles between the ER and ERGIC through classical trafficking pathways involving COPII and COPI vesicles which requires its unique C-terminal tail. We also show that syntaxin 17 is essential for maintaining the architecture of ERGIC and Golgi. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4788900 Wed, 04 May 2011 23:00:00 +0100 4788900 http://www.medworm.com/index.php?rid=4814460&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21488838%26dopt%3DAbstract Authors: Lim YS, Chua CE, Tang BL The non-motile primary cilium is a single, microtubule-based hair-like projection that emanates from most, if not all, non-dividing mammalian cells. Enriched in a variety of signalling receptors and accessories, the cilium mediates crucial sensory and regulatory functions during development and postnatal tissue homoeostasis. Maintenance of ciliary morphology and function requires continuous IFT (intraflagellar transport), and recent findings have shed light on some molecular details of how ciliogenesis is dependent on targeted exocytic membrane trafficking from the Golgi. The ARL [Arf (ADP ribosylation factor)-related] small GTPase Arf4 functions in TGN (trans-Golgi network) sorting of cilia-targeted rhodopsin into carrier vesicles, while Arl6 (Arf-lik... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4814460 Sat, 30 Apr 2011 23:00:00 +0100 4814460 http://www.medworm.com/index.php?rid=4814459&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21488839%26dopt%3DAbstract Authors: Roingeard P, Depla M LDs (lipid droplets) are probably the least well-characterized cellular organelles. Having long been considered simple lipid storage depots, they are now considered to be dynamic organelles involved in many biological processes. However, most of the mechanisms driving LDs biogenesis, growth and intracellular movement remain largely unknown. As for other cellular mechanisms deciphered through the study of viral models, HCV (hepatitis C virus) is an original and relevant model for investigations of the birth and life of these organelles. Recent studies in this model have raised the hypothesis that the HCV core protein induces the redistribution of LDs through the regression and regeneration of these organelles in specific intracellular domains. PMID: 214... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4814459 Sat, 30 Apr 2011 23:00:00 +0100 4814459 http://www.medworm.com/index.php?rid=4814458&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21524273%26dopt%3DAbstract Conclusions: The present observations support the notion that membrane rafts are involved in AChR clustering by promoting local actin cytoskeleton reorganization through the recruitment of effectors of the agrin/MuSK signaling cascade. These mechanisms are believed to play important role in vivo in the formation of the NMJ. PMID: 21524273 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4814458 Tue, 26 Apr 2011 23:00:00 +0100 4814458 http://www.medworm.com/index.php?rid=4758574&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20110018 Conclusions: The present observations support the notion that membrane rafts are involved in AChR clustering by promoting local actin cytoskeleton reorganization through the recruitment of effectors of the agrin/MuSK signaling cascade. These mechanisms are believed to play important role in vivo in the formation of the NMJ. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4758574 Tue, 26 Apr 2011 23:00:00 +0100 4758574 http://www.medworm.com/index.php?rid=4652620&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21401526%26dopt%3DAbstract This article gives an overview of the complex effects of calcium on the beating of motile cilia in the airways. PMID: 21401526 [PubMed - in process] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4652620 Wed, 30 Mar 2011 04:30:09 +0100 4652620 http://www.medworm.com/index.php?rid=4583625&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21355851%26dopt%3DAbstract Conclusions: Our observations indicate the pro-stem cell activity of cancer-associated fibroblasts and underline the role of epithelial-mesenchymal interaction in tumor biology. PMID: 21355851 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4583625 Mon, 28 Feb 2011 00:00:00 +0100 4583625 http://www.medworm.com/index.php?rid=4531977&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100113 Conclusions: Our observations indicate the pro-stem cell activity of cancer-associated fibroblasts and underline the role of epithelial-mesenchymal interaction in tumor biology. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4531977 Mon, 28 Feb 2011 00:00:00 +0100 4531977 http://www.medworm.com/index.php?rid=4583626&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21332447%26dopt%3DAbstract Conclusions. These findings suggest that (1) so called commercially available fibroblast preparations from skin (hSDFs) consist of a significant number of cells with differentiation potential aside from terminally differentiated fibroblasts; (2) colony-forming capacity and differentiation potential are specific important properties that discriminate mesenchymal stem cells from fibroblasts (WI38) while conventional stem cell properties such as plastic adherence and the expression of CD44, CD90 and CD105 are unspecific for stem cells. PMID: 21332447 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4583626 Fri, 18 Feb 2011 00:00:00 +0100 4583626 http://www.medworm.com/index.php?rid=4494838&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100117 Conclusions. These findings suggest that (1) so called commercially available fibroblast preparations from skin (hSDFs) consist of a significant number of cells with differentiation potential aside from terminally differentiated fibroblasts; (2) colony-forming capacity and differentiation potential are specific important properties that discriminate mesenchymal stem cells from fibroblasts (WI38) while conventional stem cell properties such as plastic adherence and the expression of CD44, CD90 and CD105 are unspecific for stem cells. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4494838 Fri, 18 Feb 2011 00:00:00 +0100 4494838 http://www.medworm.com/index.php?rid=4583627&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21323645%26dopt%3DAbstract Conclusion iRPSCs presented a stronger dentinogenesis but weaker osteogenesis than mRPSCs, suggesting that the dentinogenic competence of root mesenchymal stem cells decreases while their osteogenic potential increases following the maturation of tooth root. PMID: 21323645 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4583627 Thu, 17 Feb 2011 00:00:00 +0100 4583627 http://www.medworm.com/index.php?rid=4489460&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100134 Conclusion iRPSCs presented a stronger dentinogenesis but weaker osteogenesis than mRPSCs, suggesting that the dentinogenic competence of root mesenchymal stem cells decreases while their osteogenic potential increases following the maturation of tooth root. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4489460 Thu, 17 Feb 2011 00:00:00 +0100 4489460 http://www.medworm.com/index.php?rid=4411892&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21250942%26dopt%3DAbstract Authors: Sau D, Rusmini P, Crippa V, Onesto E, Bolzoni E, Ratti A, Poletti A MNDs (motorneuron diseases) are neurodegenerative disorders in which motorneurons located in the motor cortex, in the brainstem and in the spinal cord are affected. These diseases in their inherited or sporadic forms are mainly characterized by motor dysfunctions, occasionally associated with cognitive and behavioural alterations. Although these diseases show high variability in onset, progression and clinical symptoms, they share common pathological features, and motorneuronal loss invariably leads to muscle weakness and atrophy. One of the most relevant aspect of these disorders is the occurrence of defects in axonal transport, which have been postulated to be either a direct cause, or a consequence, of moto... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4411892 Sat, 29 Jan 2011 08:45:05 +0100 4411892 http://www.medworm.com/index.php?rid=4411410&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100154 Conclusions: ARAP1 interaction with CIN85 regulates endocytic trafficking of the EGFR and affects ubiquitination of EGFR. We propose a model in which the ARAP1/CIN85 complex drives exit of EGF-EGFR-Cbl complex from a pre-early endosome into a pathway distinct from the early endosome/lysosome pathway. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4411410 Fri, 28 Jan 2011 00:00:00 +0100 4411410 http://www.medworm.com/index.php?rid=4279842&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21143194%26dopt%3DAbstract Authors: Soveral G, Prista C, Moura TF, Loureiro-Dias MC In yeast, the presence of orthodox aquaporins has been first recognized in Saccharomyces cerevisiae, in which two genes (AQY1 and AQY2) were shown to be related to mammal and plant water channels. The present review summarizes the putative orthodox aquaporin protein sequences found in available genomes of yeast and filamentous fungi. Among the 28 yeast genomes sequenced, most species present only one orthodox aquaporin, and no aquaporins were found in eight yeast species. Alignment of amino acid sequences reveals a very diverse group. Similarity values vary from 99% among species within the Saccharomyces genus to 34% between ScAqy1 and the aquaporin from Debaryomyces hansenii. All of the fungal aquaporins possess the known charac... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4279842 Thu, 09 Dec 2010 00:00:00 +0100 4279842 http://www.medworm.com/index.php?rid=4252304&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21138418%26dopt%3DAbstract Conclusion. Replacement of highly conserved hydrophobic 103Gly with strongly hydrophilic 103Asp in rat AQP5, though it did not affect water permeability, may possibly have resulted in less efficient membrane trafficking and increased lysosomal degradation, leading to its lower expression in the apical membrane of the acinar cells in the SMG. PMID: 21138418 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4252304 Wed, 08 Dec 2010 00:00:00 +0100 4252304 http://www.medworm.com/index.php?rid=4241537&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100086 Conclusion. Replacement of highly conserved hydrophobic 103Gly with strongly hydrophilic 103Asp in rat AQP5, though it did not affect water permeability, may possibly have resulted in less efficient membrane trafficking and increased lysosomal degradation, leading to its lower expression in the apical membrane of the acinar cells in the SMG. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4241537 Wed, 08 Dec 2010 00:00:00 +0100 4241537 http://www.medworm.com/index.php?rid=4213681&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100083 Conclusions. These results reveal that the plant nucleus contains suggest that spectrin-like proteins s that are structural nuclear components and function as actin binding proteins. Their intranuclear distribution suggests that plant nuclear spectrin-like proteins could be involved in multiple nuclear functions. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4213681 Tue, 30 Nov 2010 00:00:00 +0100 4213681 http://www.medworm.com/index.php?rid=4196835&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100111 Conclusions. Our data therefore suggest that CDC25B associates with a centrin 2-containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of centrin 2 and a number of other centrosome components. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4196835 Tue, 23 Nov 2010 00:00:00 +0100 4196835 http://www.medworm.com/index.php?rid=4187250&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21077843%26dopt%3DAbstract Authors: Dupont N, Temime-Smaali N, Lafont F Bacterial infection relies on the micro-organism's ability to orchestrate the host's cell signalling such that the immune response is not activated. Conversely, the host cell has dedicated signalling pathways for coping with intrusions by pathogens. The autophagy of foreign micro-organisms (known as xenophagy) has emerged as one of the most powerful of these pathways, although the triggering mode remains largely unknown. In the present paper, we discuss the role that certain post-translational modifications (primarily ubiquitination) may play in the activation of xenophagy and how some bacteria have evolved mechanisms to subvert or hijack this process. In particular, we address the role played by P62/SQSTM1 (sequestosome 1). Finally, we disc... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4187250 Sun, 21 Nov 2010 01:50:20 +0100 4187250 http://www.medworm.com/index.php?rid=4187249&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21077844%26dopt%3DAbstract Authors: Zhou H, Hu H, Lai M It is widely accepted that ncRNAs (non-coding RNAs), as opposed to protein-coding RNAs, represent the majority of human transcripts; and the regulatory roles of many of these ncRNAs have been elucidated over the past decade. One important role so far recognized for ncRNAs is their participation in the epigenetic regulation of genes. Indeed, it is becoming increasingly apparent that most epigenetic mechanisms of gene expression are controlled by ncRNAs. In this review, the different types of ncRNA that are strongly linked to epigenetic regulation are characterized and their possible mechanisms discussed. PMID: 21077844 [PubMed - in process] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4187249 Sun, 21 Nov 2010 01:50:09 +0100 4187249 http://www.medworm.com/index.php?rid=4118291&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100094 Conclusions: Taken together our data suggests that TPA-induced Axl gene expression in leukemia cells is mediated by AP-1 motifs and 5xTGCGTG repeats within the promoter region -660/-580, and through the PKC/ERK1/2/AP-1 or PKC/p-38/AP-1 signalling axis (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4118291 Mon, 25 Oct 2010 23:00:00 +0100 4118291 http://www.medworm.com/index.php?rid=4108365&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20977427%26dopt%3DAbstract Conclusions: Taken together our data suggests that TPA-induced Axl gene expression in leukemia cells is mediated by AP-1 motifs and 5xTGCGTG repeats within the promoter region -660/-580, and through the PKC/ERK1/2/AP-1 or PKC/p-38/AP-1 signalling axis. PMID: 20977427 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4108365 Mon, 25 Oct 2010 23:00:00 +0100 4108365 http://www.medworm.com/index.php?rid=4097455&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100067 Conclusions. These studies support recent evidence that CtBP family proteins represent potential targets for therapeutic strategies for the treatment of cancer in general, and breast cancer in particular (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4097455 Thu, 21 Oct 2010 23:00:00 +0100 4097455 http://www.medworm.com/index.php?rid=4089077&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20964627%26dopt%3DAbstract Conclusions. These studies support recent evidence that CtBP family proteins represent potential targets for therapeutic strategies for the treatment of cancer in general, and breast cancer in particular. PMID: 20964627 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=4089077 Thu, 21 Oct 2010 23:00:00 +0100 4089077 http://www.medworm.com/index.php?rid=3982282&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20843300%26dopt%3DAbstract Conclusion Studies of epithelial carcinogenesis will benefit from culture systems which allow the manipulation of the stromal and epithelial layers independently. We have developed an organotypic culture using primary oesophageal squamous cells and fibroblasts in which a stratified epithelium with a proliferative basal layer that stains strongly for ΔNp63 develops. This model will be suitable for the study of the molecular events in the development of Barrett's oesophagus. The most commonly used normal oesophageal squamous cell line, HET-1A, does not have the characteristics of normal oesophageal squamous cells, and should not be used in models of the normal oesophageal epithelium. Until more representative cell lines are available, future studies in oesophageal cancer will be reliant o... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3982282 Tue, 14 Sep 2010 23:00:00 +0100 3982282 http://www.medworm.com/index.php?rid=3972722&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100071 Conclusion Studies of epithelial carcinogenesis will benefit from culture systems which allow the manipulation of the stromal and epithelial layers independently. We have developed an organotypic culture using primary oesophageal squamous cells and fibroblasts in which a stratified epithelium with a proliferative basal layer that stains strongly for ΔNp63 develops. This model will be suitable for the study of the molecular events in the development of Barrett’s oesophagus. The most commonly used normal oesophageal squamous cell line, HET-1A, does not have the characteristics of normal oesophageal squamous cells, and should not be used in models of the normal oesophageal epithelium. Until more representative cell lines are available, future studies in oesophageal cancer will ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3972722 Tue, 14 Sep 2010 23:00:00 +0100 3972722 http://www.medworm.com/index.php?rid=3934863&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20812916%26dopt%3DAbstract Authors: Hardy S, Legagneux V, Audic Y, Paillard L Reverse genetics consists in the modification of the activity of a target gene to analyse the phenotypic consequences. Four main approaches are used towards this goal and will be explained in this review. Two of them are centred on genome alterations. Mutations produced by random chemical or insertional mutagenesis can be screened to recover only mutants in a specific gene of interest. Alternatively, these alterations may be specifically targeted on a gene of interest by HR (homologous recombination). The other two approaches are centred on mRNA. RNA interference is a powerful method to reduce the level of gene products, while MO (morpholino) antisense oligonucleotides alter mRNA metabolism or translation. Some model species, such as D... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3934863 Sun, 05 Sep 2010 15:09:03 +0100 3934863 http://www.medworm.com/index.php?rid=3912288&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20795943%26dopt%3DAbstract In this study we describe the morphology of dormant spores using cryoelectron microscopy of vitrified sections (CEMOVIS). Biosafety measures do not permit freezing of native spores of B. anthracis without chemical fixation. To study the influence of aldehyde fixation on the ultrastructure of the spore we chose to analyse spores of the closely related non pathogen B. cereus T. For none of the investigated structures we could find a difference in morphology induced by aldehyde fixation compared to the native preparations for CEMOVIS. This result legitimises to work with aldehyde fixed spores from B. anthracis. Using CEMOVIS we describe here two new structures present in the spore: A rectangular structure, which connects the BclA filaments with the basal layer of the exosporium and a repetiti... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3912288 Wed, 25 Aug 2010 23:00:00 +0100 3912288 http://www.medworm.com/index.php?rid=3907454&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100080 In this study we describe the morphology of dormant spores using cryoelectron microscopy of vitrified sections (CEMOVIS). Biosafety measures do not permit freezing of native spores of B. anthracis without chemical fixation. To study the influence of aldehyde fixation on the ultrastructure of the spore we chose to analyse spores of the closely related non pathogen B. cereus T. For none of the investigated structures we could find a difference in morphology induced by aldehyde fixation compared to the native preparations for CEMOVIS. This result legitimises to work with aldehyde fixed spores from B. anthracis. Using CEMOVIS we describe here two new structures present in the spore: A rectangular structure, which connects the BclA filaments with the basal layer of the exosporium and a repetiti... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3907454 Wed, 25 Aug 2010 23:00:00 +0100 3907454 http://www.medworm.com/index.php?rid=3912289&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20735355%26dopt%3DAbstract Conclusions. The fragmentation of the GA and its spreading to the cell periphery is one of the key events, which promote the uniform delivery of uroplakins over the entire APM of differentiating UCs and thus have a major importance in the final proper formation and maintenance of the blood-urine barrier. PMID: 20735355 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3912289 Mon, 23 Aug 2010 23:00:00 +0100 3912289 http://www.medworm.com/index.php?rid=3899232&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100024 Conclusions. The fragmentation of the GA and its spreading to the cell periphery is one of the key events, which promote the uniform delivery of uroplakins over the entire APM of differentiating UCs and thus have a major importance in the final proper formation and maintenance of the blood-urine barrier. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3899232 Mon, 23 Aug 2010 23:00:00 +0100 3899232 http://www.medworm.com/index.php?rid=3885795&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20712599%26dopt%3DAbstract Conclusions: Our data indicate that MIICs have the capacity to fuse together, whereby the cytoskeleton possibly provides a scaffold for the MIIC shape change and directionality. MIIC-like intermediates may represent MHC class II carriers. PMID: 20712599 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3885795 Sun, 15 Aug 2010 23:00:00 +0100 3885795 http://www.medworm.com/index.php?rid=3872375&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100046 Conclusions: Our data indicate that MIICs have the capacity to fuse together, whereby the cytoskeleton possibly provides a scaffold for the MIIC shape change and directionality. MIIC-like intermediates may represent MHC class II carriers. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3872375 Sun, 15 Aug 2010 23:00:00 +0100 3872375 http://www.medworm.com/index.php?rid=3855661&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20690903%26dopt%3DAbstract Authors: Thomas J, Morlé L, Soulavie F, Laurençon A, Sagnol S, Durand B Cilia and flagella have essential functions in a wide range of organisms. Cilia assembly is dynamic during development and different types of cilia are found in multicellular organisms. How this dynamic and specific assembly is regulated remains an important question in cilia biology. In metazoans, the regulation of the overall expression level of key components necessary for cilia assembly or function is an important way to achieve ciliogenesis control. The FOXJ1 (forkhead box J1) and RFX (regulatory factor X) family of transcription factors have been shown to be important players in controlling ciliary gene expression. They fulfill a complementary and synergistic function by regulating specific and common t... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3855661 Thu, 12 Aug 2010 01:12:03 +0100 3855661 http://www.medworm.com/index.php?rid=3855660&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20690904%26dopt%3DAbstract Authors: Mastroyiannopoulos NP, Shammas C, Phylactou LA DM1 (myotonic dystrophy type I) is a common form of muscular dystrophy that affects mainly adults. It is a disease that belongs to the group of defective RNA export diseases, since a major part of the pathogenic mechanism of the disease is the retention of the mutant transcripts in the cell nucleus. The presence of an expanded CUG trinucleotide repeat in the 3'-UTR (3'-untranslated region) of the DMPK (myotonic dystrophy protein kinase) gene causes the attraction of RNA-binding proteins by the nuclear-located mutant transcripts. As a result of the occupation of the RNA-binding proteins, there is defective mis-splicing of several cellular transcripts. This is believed to be a major pathogenic mechanism of the disease and any attemp... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3855660 Thu, 12 Aug 2010 01:12:03 +0100 3855660 http://www.medworm.com/index.php?rid=3767371&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20636282%26dopt%3DAbstract Conclusion: Altogether, these data provide a new function of tPA in the central nervous system, which likely contributes to its pleiotropic functions. PMID: 20636282 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3767371 Thu, 15 Jul 2010 23:00:00 +0100 3767371 http://www.medworm.com/index.php?rid=3761306&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100072 Conclusion: Altogether, these data provide a new function of tPA in the central nervous system, which likely contributes to its pleiotropic functions. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3761306 Thu, 15 Jul 2010 23:00:00 +0100 3761306 http://www.medworm.com/index.php?rid=3761845&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20626347%26dopt%3DAbstract Conclusion.Our data suggest that inhibition of GSK3beta activity in mESCs enhances both the beta-catenin/E-cadherin mediated adhesion and the Tcf/beta-catenin-dependent transcription, but does not activate transcription in majority of the examined genes involved in epithelial-mesenchymal transition and cell cycle progression. PMID: 20626347 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3761845 Tue, 13 Jul 2010 23:00:00 +0100 3761845 http://www.medworm.com/index.php?rid=3753614&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100016 Conclusion.Our data suggest that inhibition of GSK3β activity in mESCs enhances both the β-catenin/E-cadherin mediated adhesion and the Tcf/β-catenin-dependent transcription, but does not activate transcription in majority of the examined genes involved in epithelial-mesenchymal transition and cell cycle progression. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3753614 Tue, 13 Jul 2010 23:00:00 +0100 3753614 http://www.medworm.com/index.php?rid=3722252&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20586726%26dopt%3DAbstract Authors: Polonais V, Soldati-Favre D Members of the phylum Apicomplexa are motile and rapidly dividing intracellular parasites, able to occupy a large spectrum of niches by infecting diverse hosts and invading various cell types. As obligate intracellular parasites, most apicomplexans only survive for a short period extracellularly, and, during this time, have a high energy demand to power gliding motility and invasion into new host cells. Similarly, these fast-replicating intracellular parasites are critically dependent on host-cell nutrients as energy and carbon sources, noticeably for the extensive membrane biogenesis imposed during growth and division. To access host-cell metabolites, the apicomplexans Toxoplasma gondii and Plasmodium falciparum have evolved strategies that exquisi... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3722252 Sat, 03 Jul 2010 14:09:02 +0100 3722252 http://www.medworm.com/index.php?rid=3722251&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20590528%26dopt%3DAbstract Conclusions: These results support the possibility that chromosomal instability characteristic of tumor cells may be incurred as a consequence of cell fusion and suggest the role of cell fusion in carcinogenesis may have been masked to this point for lack of an inducible method to track cell fusion. In sum, the BiFC-based approach described here allows for comprehensive studies of the mechanism and biological impact of cell fusion in nature. PMID: 20590528 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3722251 Wed, 30 Jun 2010 23:00:00 +0100 3722251 http://www.medworm.com/index.php?rid=3718212&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100033 Conclusions: These results support the possibility that chromosomal instability characteristic of tumor cells may be incurred as a consequence of cell fusion and suggest the role of cell fusion in carcinogenesis may have been masked to this point for lack of an inducible method to track cell fusion. In sum, the BiFC-based approach described here allows for comprehensive studies of the mechanism and biological impact of cell fusion in nature. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3718212 Wed, 30 Jun 2010 23:00:00 +0100 3718212 http://www.medworm.com/index.php?rid=3610638&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20504277%26dopt%3DAbstract Conclusions. Taken together, our findings establish NaF as a novel inducer of podosomes in endothelial cells, in vitro. PMID: 20504277 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3610638 Tue, 25 May 2010 23:00:00 +0100 3610638 http://www.medworm.com/index.php?rid=3599264&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100030 Conclusions. Taken together, our findings establish NaF as a novel inducer of podosomes in endothelial cells, in vitro. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3599264 Tue, 25 May 2010 23:00:00 +0100 3599264 http://www.medworm.com/index.php?rid=3600268&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20497124%26dopt%3DAbstract Conclusion: These data suggest that the inability of vesicle fusion in the growth cone affects outgrowth during the initial phases when outgrowth speed is high, but not during/after synaptogenesis. Overall, outgrowth speed is probably not rate limiting during neuronal network formation at least in vitro. In addition, Munc18, but not Munc13, regulates growth cone filopodia, potentially via its previously observed effect on filamentous actin. PMID: 20497124 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3600268 Tue, 18 May 2010 23:00:00 +0100 3600268 http://www.medworm.com/index.php?rid=3577191&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100036 Conclusion: These data suggest that the inability of vesicle fusion in the growth cone affects outgrowth during the initial phases when outgrowth speed is high, but not during/after synaptogenesis. Overall, outgrowth speed is probably not rate limiting during neuronal network formation at least in vitro. In addition, Munc18, but not Munc13, regulates growth cone filopodia, potentially via its previously observed effect on filamentous actin. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3577191 Tue, 18 May 2010 23:00:00 +0100 3577191 http://www.medworm.com/index.php?rid=3508640&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20415665%26dopt%3DAbstract Conclusions. The microscopic approach described allows visualization of protein-protein interactions involved in the assembly of 42S storage particles. In particular the transfection assay using COS-7 cells provides a rapid screening test that should facilitate identification of critical residues and structural determinants that enable the proteins of the 42S storage particle to interact with each other and to establish distinct higher-order RNP complexes. PMID: 20415665 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3508640 Sun, 25 Apr 2010 23:00:00 +0100 3508640 http://www.medworm.com/index.php?rid=3508639&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20415666%26dopt%3DAbstract Conclusions: Our results indicate that the main routes for water and solute absorption are deficient in celiac disease and may play a role in malabsorption symptoms onset. PMID: 20415666 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3508639 Sun, 25 Apr 2010 23:00:00 +0100 3508639 http://www.medworm.com/index.php?rid=3504760&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100034 Conclusions. The microscopic approach described allows visualization of protein-protein interactions involved in the assembly of 42S storage particles. In particular the transfection assay using COS-7 cells provides a rapid screening test that should facilitate identification of critical residues and structural determinants that enable the proteins of the 42S storage particle to interact with each other and to establish distinct higher-order RNP complexes. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3504760 Sun, 25 Apr 2010 23:00:00 +0100 3504760 http://www.medworm.com/index.php?rid=3504759&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100023 Conclusions: Our results indicate that the main routes for water and solute absorption are deficient in celiac disease and may play a role in malabsorption symptoms onset. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3504759 Sun, 25 Apr 2010 23:00:00 +0100 3504759 http://www.medworm.com/index.php?rid=3457220&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20377524%26dopt%3DAbstract Authors: Visvikis O, Maddugoda MP, Lemichez E Small GTPases of the Rho protein family are master regulators of the actin cytoskeleton and are targeted by potent virulence factors of several pathogenic bacteria. Their dysfunctional regulation can lead to severe human pathologies. Both host and bacterial factors can activate or inactivate Rho proteins by direct post-translational modifications: such as deamidation and transglutamination for activation, or ADP-ribosylation, glucosylation, adenylylation and phosphorylation for inactivation. We review and compare these unconventional ways in which both host cells and bacterial pathogens regulate Rho proteins. PMID: 20377524 [PubMed - in process] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3457220 Sun, 11 Apr 2010 04:54:03 +0100 3457220 http://www.medworm.com/index.php?rid=3457219&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20377525%26dopt%3DAbstract Authors: Vieira FS, Corrêa G, Einicker-Lamas M, Coutinho-Silva R The lipid raft hypothesis proposed that these microdomains are small (10-200 nM), highly dynamic and enriched in cholesterol, glycosphingolipids and signalling phospholipids, which compartmentalize cellular processes. These membrane regions play crucial roles in signal transduction, phagocytosis and secretion, as well as pathogen adhesion/interaction. Throughout evolution, many pathogens have developed mechanisms to escape from the host immune system, some of which are based on the host membrane microdomain machinery. Thus lipid rafts might be exploited by pathogens as signalling and entry platforms. In this review, we summarize the role of lipid rafts as players in the overall invasion process used by different path... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3457219 Sun, 11 Apr 2010 04:54:03 +0100 3457219 http://www.medworm.com/index.php?rid=3457218&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20377528%26dopt%3DAbstract Conclusions. In turbot hepatocytes, hypo-osmotic swelling but not hyper-osmotic shrinkage induced the activation of ERK1/2. However these proteins do not seem to be involved in RVD process. Their hypo-osmotic-induced activation is partially due to cascades of signalling events triggered by the binding of released ATP on purinergic P2 receptors and requires the presence of calcium. PMID: 20377528 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3457218 Thu, 08 Apr 2010 23:00:00 +0100 3457218 http://www.medworm.com/index.php?rid=3435309&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20356318%26dopt%3DAbstract Authors: Ono A Advances in cell biology and biophysics revealed that cellular membranes consist of multiple microdomains with specific sets of components such as lipid rafts and TEMs (tetraspanin-enriched microdomains). An increasing number of enveloped viruses have been shown to utilize these microdomains during their assembly. Among them, association of HIV-1 (HIV type 1) and other retroviruses with lipid rafts and TEMs within the PM (plasma membrane) is well documented. In this review, I describe our current knowledge on interrelationships between PM microdomain organization and the HIV-1 particle assembly process. Microdomain association during virus particle assembly may also modulate subsequent virus spread. Potential roles played by microdomains will be discussed with regard to ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3435309 Sat, 03 Apr 2010 16:56:02 +0100 3435309 http://www.medworm.com/index.php?rid=3362873&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20222872%26dopt%3DAbstract In conclusion, the discovery of novel interactors has and will extend our knowledge of the biological roles of ESCRTs. PMID: 20222872 [PubMed - in process] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3362873 Sun, 14 Mar 2010 03:50:04 +0100 3362873 http://www.medworm.com/index.php?rid=3331336&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20196772%26dopt%3DAbstract Conclusions. The data presented in the present study identify a novel organelle in chicken egg yolk composed of acidic vesicles with morphology, physiology, and composition similar to those of acidocalcisomes, within larger acidic vacuoles. The elemental composition of these acidocalcisomes is proportionally similar to the elemental composition of the yolk suggesting that most of these elements are located in these organelles, which might be an important storage compartment in eggs. PMID: 20196772 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3331336 Wed, 03 Mar 2010 00:00:00 +0100 3331336 http://www.medworm.com/index.php?rid=3326788&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100011 Conclusions. The data presented in the present study identify a novel organelle in chicken egg yolk composed of acidic vesicles with morphology, physiology, and composition similar to those of acidocalcisomes, within larger acidic vacuoles. The elemental composition of these acidocalcisomes is proportionally similar to the elemental composition of the yolk suggesting that most of these elements are located in these organelles, which might be an important storage compartment in eggs. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3326788 Wed, 03 Mar 2010 00:00:00 +0100 3326788 http://www.medworm.com/index.php?rid=3303375&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20170475%26dopt%3DAbstract Conclusion. Our results suggest that MT7 toxin binds to a dimeric form of hM1 receptor, favouring the stability of this receptor state at the cellular level, probably by inducing some conformational rearrangements of the pre-existing muscarinic receptor homodimers. PMID: 20170475 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3303375 Mon, 22 Feb 2010 00:00:00 +0100 3303375 http://www.medworm.com/index.php?rid=3294462&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090171 Conclusion. Our results suggest that MT7 toxin binds to a dimeric form of hM1 receptor, favouring the stability of this receptor state at the cellular level, probably by inducing some conformational rearrangements of the pre-existing muscarinic receptor homodimers. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3294462 Mon, 22 Feb 2010 00:00:00 +0100 3294462 http://www.medworm.com/index.php?rid=3270060&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20146669%26dopt%3DAbstract Authors: Lanyon-Hogg T, Warriner SL, Baker A Peroxisomes are a family of organelles which have many unusual features. They can arise de novo from the endoplasmic reticulum by a still poorly characterized process, yet possess a unique machinery for the import of their matrix proteins. As peroxisomes lack DNA, their function, which is highly variable and dependent on developmental and/or environmental conditions, is determined by the post-translational import of specific metabolic enzymes in folded or oligomeric states. The two classes of matrix targeting signals for peroxisomal proteins [PTS1 (peroxisomal targeting signal 1) and PTS2] are recognized by cytosolic receptors [PEX5 (peroxin 5) and PEX7 respectively] which escort their cargo proteins to, or possibly across, the peroxisome me... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3270060 Sun, 14 Feb 2010 01:28:03 +0100 3270060 http://www.medworm.com/index.php?rid=3213260&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20100171%26dopt%3DAbstract Conclusions. Our vectorial concept proposes a new chemical approach for traffic-based profiling of proteins that may prove to be applicable to the analysis of diverse endocytic pathways. PMID: 20100171 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3213260 Mon, 25 Jan 2010 00:00:00 +0100 3213260 http://www.medworm.com/index.php?rid=3204697&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20100008 Conclusions. Our vectorial concept proposes a new chemical approach for traffic-based profiling of proteins that may prove to be applicable to the analysis of diverse endocytic pathways. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3204697 Mon, 25 Jan 2010 00:00:00 +0100 3204697 http://www.medworm.com/index.php?rid=3213261&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20095965%26dopt%3DAbstract Conclusions. Our observations, supported by biochemical data, provide evidence for the involvement of sphingomyelin in important nuclear functions. They bring additional information pointing out the perichromatin region as an essential functional nuclear domain. Furthermore, they suggest a role for sphingomyelin in the internal nuclear architecture. PMID: 20095965 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3213261 Fri, 22 Jan 2010 00:00:00 +0100 3213261 http://www.medworm.com/index.php?rid=3200359&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090139 Conclusions. Our observations, supported by biochemical data, provide evidence for the involvement of sphingomyelin in important nuclear functions. They bring additional information pointing out the perichromatin region as an essential functional nuclear domain. Furthermore, they suggest a role for sphingomyelin in the internal nuclear architecture. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3200359 Fri, 22 Jan 2010 00:00:00 +0100 3200359 http://www.medworm.com/index.php?rid=3189551&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20078424%26dopt%3DAbstract Conclusions: We have shown that endogenous stefin B aggregates within cells, and that aggregation is increased upon protein over-expression or proteasome inhibition. From confocal and TEM analyses we conclude that aggregates of stefin B show some of the molecular characteristics of aggresomes and may be eliminated from the cell by autophagy. Intracellular stefin B aggregation shows a negative correlation with cell survival. PMID: 20078424 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3189551 Fri, 15 Jan 2010 00:00:00 +0100 3189551 http://www.medworm.com/index.php?rid=3175729&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090163 Conclusions: We have shown that endogenous stefin B aggregates within cells, and that aggregation is increased upon protein over-expression or proteasome inhibition. From confocal and TEM analyses we conclude that aggregates of stefin B show some of the molecular characteristics of aggresomes and may be eliminated from the cell by autophagy. Intracellular stefin B aggregation shows a negative correlation with cell survival. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3175729 Fri, 15 Jan 2010 00:00:00 +0100 3175729 http://www.medworm.com/index.php?rid=3164636&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20059450%26dopt%3DAbstract Authors: Michelet X, Djeddi A, Legouis R ESCRTs (endosomal sorting complexes required for transport) were first discovered in yeast and are known to be required in the biogenesis of the MVB (multivesicular body). Most ESCRT research has been carried out in vitro using models such as yeast and mammalian cells in culture. The role of the ESCRTs genes in endosome maturation is conserved from yeast to mammals, but little is known about their function during development in multicellular organisms. Since ESCRTs play a leading role in regulating some cell signalling pathways by addressing receptors to the lysosome, it appears important to monitor ESCRT functions in multicellular models. The present review summarizes recent research on the developmental and cellular functions of the ESCRT in C... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3164636 Wed, 13 Jan 2010 08:32:02 +0100 3164636 http://www.medworm.com/index.php?rid=3044478&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090098 Branchio-Oto-Renal (BOR) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 gene. Symptoms commonly include deafness and renal anomalies. We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to examine the effects of different EYA1 mutations. Four eya1 mRNAs encoding proteins correlated with congenital anomalies in human were injected into early stage embryos. We show that expression of mutations associated with BOR, even in the presence of normal levels of endogenous eya1 mRNA, leads to morphologically abnormal ear development as measured by overall otic vesicle size, establishment of sensory tissue and ear otic innervation patterns. The molecular consequences of mutant eya1 expression were assessed by quantitative PCR... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3044478 Tue, 01 Dec 2009 00:00:00 +0100 3044478 http://www.medworm.com/index.php?rid=3012215&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090141 Conclusions Taken together, these findings demonstrate that repression, complex post-transcriptional regulation and modulation of mRNA stability could potentially be taking place along the distal edge of migrating lamellipodia. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3012215 Fri, 20 Nov 2009 00:00:00 +0100 3012215 http://www.medworm.com/index.php?rid=3004147&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19909237%26dopt%3DAbstract Authors: Carmosino M, Valenti G, Caplan M, Svelto M Polarity is the structural and functional hallmark of epithelia. The apical plasma membrane, facing the organism's exterior (the lumen of the gut, renal tubule and glandular duct), differs in many important respects from the basolateral plasma membrane that is apposed to the interior of the organism. The generation and maintenance of epithelial polarity require a highly specialized subcellular machinery to bring proteins to their appropriate sites of action. This is a dynamic process involving the interpretation of sorting signals, vectorial delivery mechanisms, membrane-specific fusion and retention processes. Here, we will provide a review of the field, highlighting recent advances within a historically relevant context. PMID: 1... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=3004147 Wed, 18 Nov 2009 17:46:05 +0100 3004147 http://www.medworm.com/index.php?rid=2992604&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090074 Conclusions. Our results show that endogenous GIT complexes are involved in the regulation of chemoattractant-induced cell motility and receptor trafficking, and support previous findings indicating an important function of the GIT complexes in the regulation of different G protein-coupled receptors. Our data also indicate that endogenous GIT1 and GIT2 regulate distinct subsets of agonist-induced responses, and suggest a possible functional link between the control of receptor trafficking and the regulation of cell motility by GIT proteins. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2992604 Fri, 13 Nov 2009 00:00:00 +0100 2992604 http://www.medworm.com/index.php?rid=2984660&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090033 Conclusions Our findings shows that CIP4-like proteins induced membrane tubulation downstream of Cdc42 and that they have important roles in PDGF-dependent actin reorganization and cell migration by regulating internalization and activity of the PDGFRβ. Moreover, the data suggest an important role for the CIP4-like proteins in the regulation of the activity of the PDGF β-receptor. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2984660 Thu, 12 Nov 2009 00:00:00 +0100 2984660 http://www.medworm.com/index.php?rid=2973799&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090132 Conclusion. Estrogen responsive cell line with ERα and CD44+/CD24-/low expression can be derived from breast epithelial stem cells. The tumorigenicity and estrogen response of these cells could depend on cell culture condition. The findings of this study have implications in regard to the origins of 1) ERα-positive breast cancers, 2) CD44+/CD24-/low breast tumor stem cells and 3) metastatic ability of breast cancer. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2973799 Mon, 09 Nov 2009 00:00:00 +0100 2973799 http://www.medworm.com/index.php?rid=2958717&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090091 Conclusion: We demonstrate here that TLRR interacts with PP1, particularly the testis specific isoform, PP1γ2. Immunoaffinity purification confirms that TLRR is associated with the spermatid cytoskeleton. In addition, proteins involved in protein stability are part of the TLRR complex. These results support our hypothesis that TLRR links signaling molecules to the spermatid cytoskeleton in order to regulate important substrates involved in spermatid transformation. The translocation of TLRR from manchette to the centrosome region suggests a possible role for this protein in tail formation. Our finding that TLRR is associated with microtubules in cultured cells suggests that TLRR may play a common role in modulating the cytoskeleton in other cell types besides male germ cells. (Sourc... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2958717 Wed, 04 Nov 2009 00:00:00 +0100 2958717 http://www.medworm.com/index.php?rid=2950596&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090104 Conclusions. We demonstrate that FAK reduces steady-state adhesion strength by modulating vinculin recruitment to focal adhesions. These findings provide insights into the role of FAK in mechanical interactions between a cell and the extracellular matrix. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2950596 Mon, 02 Nov 2009 00:00:00 +0100 2950596 http://www.medworm.com/index.php?rid=2935729&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19857204%26dopt%3DAbstract Authors: Popescu CI, Dubuisson J HCV (hepatitis C virus) represents a major global health problem. A consistent body of evidence has been accumulating, suggesting a peculiar overlap between the HCV life cycle and lipid metabolism. This association becomes evident both for the clinical symptoms of HCV infection and the molecular mechanisms underlying the morphogenesis and entry process of this virus. The HCV core-lipid droplets association seems to be central to the HCV morphogenesis process. Moreover, the biogenesis pathway of very-low-density lipoproteins has been shown to be involved in HCV morphogenesis with MTP (microsomal triacylglycerol transfer protein), ApoB (apolipoprotein B) and ApoE (apolipoprotein E) as essential elements in the production of infectious HCV particles. HCV i... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2935729 Thu, 29 Oct 2009 09:08:05 +0100 2935729 http://www.medworm.com/index.php?rid=2894345&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090122 Conclusions: During oral and dental development ADM initially localises to epithelial tissue whilst during later stages is present in mineralised secreting cells, including odontoblasts. ADM may regulate proliferation and mineralisation processes during development whilst in adulthood, it may be important in maintaining dental tissue homeostasis. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2894345 Tue, 13 Oct 2009 23:00:00 +0100 2894345 http://www.medworm.com/index.php?rid=2807452&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090096 Conclusions: We believe that this technique will contribute to correlative studies in multicellular models, and will facilitate the time-demanding procedure of specimen preparation for any kind of TEM. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2807452 Thu, 17 Sep 2009 23:00:00 +0100 2807452 http://www.medworm.com/index.php?rid=2807451&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090073 Conclusions: Taken together, our results suggest that elevated levels of sPLA2 secreted by wing bud mesenchymal cells promote type I collagen degradation by MMP-9 in a manner typical of receptor mediation and that these events lead to apoptotic cell death. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2807451 Thu, 17 Sep 2009 23:00:00 +0100 2807451 http://www.medworm.com/index.php?rid=2789383&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19743965%26dopt%3DAbstract Authors: Chang ZL Monocytes/macrophages are critical for both immunity and homoeostasis. They are the outposts of the immune system in detecting invading pathogens or foreign antigens for homoeostatic clearance and antigen processing for the initiation and effector stages of both innate and adaptive immunity. In addition, monocytes/macrophages often function as control switches for immune system balance between pro- and anti-inflammatory reactions. In the beginning of this article, I would like to briefly introduce the achievements of Metchnikoff and Ehrlich in immunology, including Metchnikoff's cell theory, since they have both greatly influenced the advancement of modern immunology. Additionally, I will honour the 100th anniversary of the 1908 Nobel Prize in Physiology or Medicine. ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2789383 Sun, 13 Sep 2009 03:02:06 +0100 2789383 http://www.medworm.com/index.php?rid=2729960&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090068 Conclusion The data presented here, which identifies the class I FIPs as the first putative effector proteins for the Rab14 GTPase, indicates greater complexity in the Rab-binding specificity of the class I FIP proteins. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2729960 Mon, 24 Aug 2009 23:00:00 +0100 2729960 http://www.medworm.com/index.php?rid=2713521&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19686094%26dopt%3DAbstract Authors: Segurado M, Tercero JA The S-phase checkpoint is a surveillance mechanism, mediated by the protein kinases Mec1 and Rad53 in the budding yeast Saccharomyces cerevisiae (ATR and Chk2 in human cells, respectively) that responds to DNA damage and replication perturbations by co-ordinating a global cellular response necessary to maintain genome integrity. A key aspect of this response is the stabilization of DNA replication forks, which is critical for cell survival. A defective checkpoint causes irreversible replication-fork collapse and leads to genomic instability, a hallmark of cancer cells. Although the precise mechanisms by which Mec1/Rad53 maintain functional replication forks are currently unclear, our knowledge about this checkpoint function has significantly increased du... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2713521 Thu, 20 Aug 2009 06:06:05 +0100 2713521 http://www.medworm.com/index.php?rid=2708928&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090099 Conclusions. This study is the first report of the identification, annotation, and expression analysis of the mouse Shtap gene. The redistribution observed during sperm capacitation raises the possibility that SHTAP and the SHTAP-CRISP2 complex play a role in the attainment of sperm functional competence. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2708928 Mon, 17 Aug 2009 23:00:00 +0100 2708928 http://www.medworm.com/index.php?rid=2674160&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090056 Conclusions. These data show that interleukin-1β acts through the extracellular signal regulated kinase signaling cascade to alter the expression and function of connexin43 in synovial fibroblasts. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2674160 Wed, 05 Aug 2009 23:00:00 +0100 2674160 http://www.medworm.com/index.php?rid=2661084&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19642968%26dopt%3DAbstract Authors: Zhao Q, Ren H, Zhu D, Han Z Morbidity and mortality from cirrhosis is increasing rapidly in the world. Currently, orthotopic liver transplantation is the only definitive therapeutic option. However, its clinical use is limited, because of poor long-term graft survival, donor organ shortage and high costs associated with the procedure. Stem cell replacement strategies are therefore being investigated as an attractive alternative approach to liver repair and regeneration. In this review we discuss recent preclinical and clinical investigations that explore the therapeutic potential of stem cells in repair of liver injuries. Several types of stem cells. including embryonic stem cells, haematopoietic stem cells and mesenchymal stem cells, can be induced to differentiate into hepat... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2661084 Sat, 01 Aug 2009 19:14:05 +0100 2661084 http://www.medworm.com/index.php?rid=2641240&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090077 Conclusions. These results show that the functional integrity of the nuclear envelope and plasma membrane may be conserved until the end of the execution phase of apoptosis. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2641240 Sun, 26 Jul 2009 23:00:00 +0100 2641240 http://www.medworm.com/index.php?rid=2580064&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090048 Conclusion: We conclude that Cdc42 does not participate in the early events that initiate stable cadherin adhesion in keratinocytes. Yet, it is feasible that Cdc42 may be activated at later time points or by other receptors. Cdc42 can then participate in additional functions during polarization such as Golgi re-positioning or baso-lateral trafficking. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2580064 Mon, 06 Jul 2009 23:00:00 +0100 2580064 http://www.medworm.com/index.php?rid=2563027&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19566487%26dopt%3DAbstract Authors: Strating JR, Martens GJ The secretory pathway is of vital importance for eukaryotic cells and has a pivotal role in the synthesis, sorting, processing and secretion of a large variety of bioactive molecules involved in intercellular communication. One of the key processes in the secretory pathway concerns the transport of cargo proteins from the ER (endoplasmic reticulum) to the Golgi. Type-I transmembrane proteins of approximately 24 kDa are abundantly present in the membranes of the early secretory pathway, and bind the COPI and COPII coat complexes that cover vesicles travelling between the membranes. These p24 proteins are thought to play an important role in the selective transport processes at the ER-Golgi interface, although their exact functioning is still obscure. One... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2563027 Fri, 03 Jul 2009 07:04:05 +0100 2563027 http://www.medworm.com/index.php?rid=2555985&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090070 Conclusions. These data indicate that STAT3 functions as a critical factor for adipogenesis via a mechanism involving the PPARγ activation pathway. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2555985 Mon, 29 Jun 2009 23:00:00 +0100 2555985 http://www.medworm.com/index.php?rid=2527279&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19419346%26dopt%3DAbstract Authors: Sýkorová E, Fajkus J The TERT (telomerase reverse transcriptase) subunit of telomerase is an intensively studied macromolecule due to its key importance in maintaining genome integrity and role in cellular aging and cancer. In an effort to provide an up-to-date overview of the topic, we discuss the structure of TERT genes, their alternative splicing products and their functions. Nucleotide databases contain more than 90 full-length cDNA sequences of telomerase protein subunits. Numerous in silico, in vitro and in vivo experimental techniques have revealed a great deal of structural and functional data describing particular features of the telomerase subunit in various model organisms. We explore whether particular findings are generally applicable to telomerases or s... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2527279 Sat, 27 Jun 2009 11:53:26 +0100 2527279 http://www.medworm.com/index.php?rid=2527268&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19527220%26dopt%3DAbstract Conclusions: Integrin alpha8beta1 is expressed in epithelial cells. In intestinal crypt cells, alpha8beta1 is closely involved in the regulation of adhesion, migration and cell proliferation via a predominant RhoA/ROCK dependent mechanism. These results suggest an important role for this integrin in intestinal crypt cell homeostasis. PMID: 19527220 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2527268 Sun, 14 Jun 2009 23:00:00 +0100 2527268 http://www.medworm.com/index.php?rid=2508634&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090060 Conclusions: Integrin α8β1 is expressed in epithelial cells. In intestinal crypt cells, α8β1 is closely involved in the regulation of adhesion, migration and cell proliferation via a predominant RhoA/ROCK dependent mechanism. These results suggest an important role for this integrin in intestinal crypt cell homeostasis. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2508634 Sun, 14 Jun 2009 23:00:00 +0100 2508634 http://www.medworm.com/index.php?rid=2527270&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19522705%26dopt%3DAbstract Conclusion: Inhibition of Crm1 in early metaphase favors the formation of supplementary acentriolar spindle poles. B23 and NUMA are present at these poles that ultimately focus around the centrosome. Inhibition of Crm1 at metaphase has no effect on the control of centrosome numbers. PMID: 19522705 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2527270 Thu, 11 Jun 2009 23:00:00 +0100 2527270 http://www.medworm.com/index.php?rid=2508635&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20080218 Conclusion: Inhibition of Crm1 in early metaphase favors the formation of supplementary acentriolar spindle poles. B23 and NUMA are present at these poles that ultimately focus around the centrosome. Inhibition of Crm1 at metaphase has no effect on the control of centrosome numbers. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2508635 Thu, 11 Jun 2009 23:00:00 +0100 2508635 http://www.medworm.com/index.php?rid=2527271&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19515017%26dopt%3DAbstract Conclusions. These data suggest that Ptena454 regulation is correlated to changes in phospho-Akt levels. We propose a model by which homeostasis in rapidly dividing and migrating embryonic cells depends on a counterbalance between pro-survival signaling employing CK2 and Gsk-3 and the pro-apoptotic activity of Ptena454. PMID: 19515017 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2527271 Tue, 09 Jun 2009 23:00:00 +0100 2527271 http://www.medworm.com/index.php?rid=2463698&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090042 Conclusion. The present study highlights the role of Wnt5α signal in notochord convergent extension movements in ascidian embryos. Our results raise the novel possibility that Wnt5α functions in a cell-autonomous manner in activation of the Wnt/PCP pathway to polarize the protrusive activity that drives convergent extension. (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2463698 Mon, 08 Jun 2009 04:00:00 +0100 2463698 http://www.medworm.com/index.php?rid=2527273&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19505288%26dopt%3DAbstract Conclusion. The present study highlights the role of Wnt5alpha signal in notochord convergent extension movements in ascidian embryos. Our results raise the novel possibility that Wnt5alpha functions in a cell-autonomous manner in activation of the Wnt/PCP pathway to polarize the protrusive activity that drives convergent extension. PMID: 19505288 [PubMed - as supplied by publisher] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2527273 Sun, 07 Jun 2009 23:00:00 +0100 2527273 http://www.medworm.com/index.php?rid=2527283&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19356152%26dopt%3DAbstract Authors: Lankat-Buttgereit B, Göke R Pdcd4 (programmed cell death 4) has been known as a tumour suppressor gene and potential target for anticancer therapies for several years. Initially, Pdcd4 was identified as a gene that is up-regulated during apoptosis, but its precise role still remains to be defined. However, there is increasing evidence that Pdcd4 levels influence transcription, as well as translation, modulate different signal transduction pathways and might act as a tumour suppressor. Interestingly, recent data suggest that Pdcd4 function may depend on cell type and/or genetic background. This review summarizes the current knowledge regarding the function and regulation of Pdcd4. PMID: 19356152 [PubMed - indexed for MEDLINE] (Source: Biology of the Cell) Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2527283 Sun, 31 May 2009 23:00:00 +0100 2527283 http://www.medworm.com/index.php?rid=2423500&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090015 The effects of Cd on the morphology and function of the chloroplast and mitochondria, as well as on the production and localization of reactive oxygen species (ROS) were studied at the single-cell level in Arabidopsis. Results demonstrated that the morphology of chloroplast changed after Cd treatment, and photochemical efficiency dramatically declined prior to obvious morphological distortion in the chloroplast. A quick burst of ROS was detected after Cd treatment. The ROS appeared first in the mitochondria and subsequently in the chloroplast. Simultaneously, the mitochondria clumped irregularly around the chloroplasts or aggregated in the cytoplasm, and the movement of mitochondria was concomitantly blocked. Furthermore, ROS production could be decreased after pre-treatment with ascorbic ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2423500 Tue, 19 May 2009 04:00:00 +0100 2423500 http://www.medworm.com/index.php?rid=2527275&cid=s_34549_171_f&fid=34549&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19453296%26dopt%3DAbstract Authors: Bi Y, Chen W, Zhang W, Zhou Q, Yuan L, Xing D The effects of Cd on the morphology and function of the chloroplast and mitochondria, as well as on the production and localization of reactive oxygen species (ROS) were studied at the single-cell level in Arabidopsis. Results demonstrated that the morphology of chloroplast changed after Cd treatment, and photochemical efficiency dramatically declined prior to obvious morphological distortion in the chloroplast. A quick burst of ROS was detected after Cd treatment. The ROS appeared first in the mitochondria and subsequently in the chloroplast. Simultaneously, the mitochondria clumped irregularly around the chloroplasts or aggregated in the cytoplasm, and the movement of mitochondria was concomitantly blocked. Furthermore, ROS produ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2527275 Mon, 18 May 2009 23:00:00 +0100 2527275 http://www.medworm.com/index.php?rid=2416197&cid=s_34549_171_f&fid=37622&url=http%3A%2F%2Fwww.biolcell.org%2Fboc%2Fimps%2Frefer.htm%3FMSID%3DBC20090025 Within the endocytic pathway, the Endosomal Sorting Complex Required for Transport (ESCRT) machinery is essential for the biogenesis of MultiVesicular Bodies (MVB). In yeast, ESCRTs are recruited at the endosomal membrane and involved in cargo sorting into intralumenal vesicles of the MVB. Here, we characterize the ESCRT-III protein CeVPS-32 in the nematode C. elegans and its interactions with CeVPS-27, CeVPS-23 and CeVPS-4. In contrast to other CevpsE genes, depletion of Cevps-32 is embryonic lethal with severe defects in the remodelling of epithelial cell shape during organogenesis. Furthermore, Cevps-32 animals display an accumulation of enlarged early endosomes in epithelial cells and an accumulation of autophagosomes. CeVPS-32 protein is enriched in epithelial tissues and in residual ... Biology of the Cell journals http://www.medworm.com/rss/comments.php?id=2416197 Tue, 12 May 2009 04:00:00 +0100 2416197