MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Biotechnology Journal' source. Sat, 20 Mar 2010 16:11:41 +0100 http://www.medworm.com/index.php?rid=3379828&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20235143%26dopt%3DAbstract Authors: Mazars A, Fåhraeus R Modification of protein-protein interactions (PPIs) holds promise for novel rational drug design. Disrupting or modifying protein interactions offers new challenges in terms of chemical compound libraries and techniques for compound validation. As proteins interact with several partners in different allosteric conformation in a pathological and tissue-specific fashion, it is difficult to predict the in vivo effect of PPI acting compounds identified by in vitro screening assays. It is therefore desirable to develop techniques that rapidly allow cell-based validation of protein interacting compounds. The binding of the p53 tumor suppressor to the HDM2 E3 ubiquitin ligase is important for controlling p53 activity, and several compounds, such as Nutlin-3,... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3379828 Tue, 16 Mar 2010 00:00:00 +0100 3379828 http://www.medworm.com/index.php?rid=3379827&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20235144%26dopt%3DAbstract Authors: Marques MP, Cabral JM, Fernandes P The microwell-scale approach is widely used for screening purposes and one-pot biotransformations, but it has seldom been applied to complex whole cell multistep bioconversions, requiring prolonged incubation periods. The present study aims to contribute to filling this gap. The side-chain cleavage of sitosterol to androstenedione (AD) with Mycobacterium sp. NRRL B-3805 cells was used as a model system, and focus was given to the screening of suitable bioconversion media with 24-well microwell plates. Results show that to perform this particular bioconversion growing cells are preferred over resting cells due to higher conversion yields obtained in aqueous medium. The use of resting cells may nevertheless present an interesting approach provi... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3379827 Tue, 16 Mar 2010 00:00:00 +0100 3379827 http://www.medworm.com/index.php?rid=3379826&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20235145%26dopt%3DAbstract Authors: Kitazoe M, Futami J, Nishikawa M, Yamada H, Maeda Y The Wnt canonical signaling pathway is essential for the early development of eukaryotic organisms and plays a key role in cell proliferation, differentiation, and oncogenesis. Moreover, the Wnt canonical signaling pathway contributes to the self-renewal of mouse hematopoietic stem cells (HSCs). Here, we demonstrate artificial activation of the Wnt canonical signaling pathway by beta-catenin protein transduction. Constitutively active beta-catenin protein was introduced into human embryonic kidney HEK-293 cells using a polyethylenimine (PEI) cationization method, or with the BioPORTER protein transduction reagent. We have previously shown that modification with PEI effectively causes proteins to be internalized by living mamm... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3379826 Tue, 16 Mar 2010 00:00:00 +0100 3379826 http://www.medworm.com/index.php?rid=3362737&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20222103%26dopt%3DAbstract Authors: Hung F, Deng L, Ravnikar P, Condon R, Li B, Do L, Saha D, Tsao YS, Merchant A, Liu Z, Shi S The productivity of stably transfected cell lines is of critical importance for the manufacturing of therapeutic proteins. Various methods have been successfully implemented to increase the production output of mammalian cell cultures. Increasing evidence suggests that optimization of the gene coding sequences of an expression vector can improve specific cell line yield of the recombinant protein. Here we demonstrate that gene optimization substantially enhances antibody production in Chinese hamster ovary cells. When gene optimization was applied to the heavy and light chain genes of a therapeutic antibody, we observed increased antibody production in transient transfection. Elevated h... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3362737 Wed, 10 Mar 2010 00:00:00 +0100 3362737 http://www.medworm.com/index.php?rid=3362736&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20222104%26dopt%3DAbstract Authors: Fukuda T, Tsuchiya K, Makishima H, Tsuchiyama K, Mulchandani A, Kuroda K, Ueda M, Suye SI Organophosphorus compounds (OPs) such as pesticides, fungicides, and herbicides are highly toxic but are nevertheless extensively used worldwide. To detect OPs, we constructed a yeast strain that co-displays organophosphorus hydrolase (OPH) and enhanced green fluorescent protein (EGFP) on the cell surface using a Flo1p anchor system. OP degradation releases protons and causes a change in pH. This pH change results in structural deformation of EGFP, which triggers quenching of its fluorescence, thereby making this cell useful for visual detection of OPs. Fluorescence microscopy confirmed the high-intensity fluorescence displayed by EGFP on the cell surface. The yeast strain possessed suffi... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3362736 Wed, 10 Mar 2010 00:00:00 +0100 3362736 http://www.medworm.com/index.php?rid=3362735&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20222105%26dopt%3DAbstract Authors: Sung K, Kamiya N, Kawata N, Kamiya S, Goto M A chemically modified glass surface displaying a glutamyl donor substrate peptide (Z-QG) was developed for microbial transglutaminase (MTG)-mediated immobilization of recombinant proteins tagged with an MTG-reactive lysine-containing substrate peptide (K-tag). To evaluate the surface modification conditions affecting the enzymatic protein immobilization, we employed an amino-modified 96-well glass plate as a base and prepared three types of glass surfaces displaying Z-QG. Validation of the Z-QG modified glass surfaces with recombinant enhanced green fluorescent proteins revealed that the insertion of a di(ethylene glycol) linker between the terminal Z-QG moiety and the base not only enhances enzymatic protein immobilization efficien... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3362735 Wed, 10 Mar 2010 00:00:00 +0100 3362735 http://www.medworm.com/index.php?rid=3354917&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213634%26dopt%3DAbstract Authors: no abstract. PMID: 20213634 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354917 Mon, 08 Mar 2010 00:00:00 +0100 3354917 http://www.medworm.com/index.php?rid=3354916&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213635%26dopt%3DAbstract Authors: Carioca JO no abstract. PMID: 20213635 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354916 Mon, 08 Mar 2010 00:00:00 +0100 3354916 http://www.medworm.com/index.php?rid=3354915&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213636%26dopt%3DAbstract Authors: Nielsen DR, Yoon SH, Yuan CJ, Prather KL The functional reconstruction of acetoin and meso-2,3-butanediol (meso-2,3-BD) biosynthetic pathways in Escherichia coli have been explored systematically. Pathway construction involved the in vsivo screening of prospective pathway isozymes of yeast and bacterial origin. After substantial engineering of the host background to increase pyruvate availability, E. coli YYC202(DE3) ldhA(() ilvC( expressing ilvBN from E. coli and aldB from L. lactis (encoding acetolactate synthase and acetolactate decarboxylase activities, respectively) was able to produce up to 870 mg/L acetoin, with no coproduction of diacetyl observed. These strains were also found to produce small quantities of meso-2,3-BD, suggesting the existence of endogenous 2,3-BD de... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354915 Mon, 08 Mar 2010 00:00:00 +0100 3354915 http://www.medworm.com/index.php?rid=3354914&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213637%26dopt%3DAbstract Authors: News: Attraction of adipose tissue - Swine flu vaccine from insectcells - MicroRNA in multiple sclerosis - Proteomic detection of adenoma - Systematic engineering for arsenic removal - Bacterial cellulose to make blood vessels - The recipe for primordial soup: acid or bitter, hot or warm - Increased O(2) in hollow fiber bioreactors - Eye stem cells to treat immune disease - Super-strong collagen - Designing functional metalloproteins - Higher oil content in tobacco leaves - Alternative metabolic routes - New compass point blot helps find hTERT factors - Extracellular signals - doomed cells in the bacterial communityJournal Highlights: Oils and fats for the chemical industry - Molecular Nutrition ReviewsNew from the Encyclopedia of Life Sciences:EM analysis of protein structur... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354914 Mon, 08 Mar 2010 00:00:00 +0100 3354914 http://www.medworm.com/index.php?rid=3354913&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213638%26dopt%3DAbstract Authors: Cover illustration: Focus: Microbial Biotech. The cover image shows colored E. coli, the most commonly used microbes for a variety of biotechnological applications. In this issue, Nielsen et al., metabolically engineered E. coli to produce butanediol and acetoin, which can be used as chemical precursors for many industrial products (pp. 274-284). http://dx.doi.org/10.1002/biot.200900279. PMID: 20213638 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354913 Mon, 08 Mar 2010 00:00:00 +0100 3354913 http://www.medworm.com/index.php?rid=3354912&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213639%26dopt%3DAbstract Authors: Lee SY no abstract. PMID: 20213639 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354912 Mon, 08 Mar 2010 00:00:00 +0100 3354912 http://www.medworm.com/index.php?rid=3354911&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213640%26dopt%3DAbstract Authors: METABOLIC ENGINEERING FOR INDUSTRIAL APPLICATIONS: Nielsen et al., Biotechnol. J. 2009, 5, 274-284With a carbon chain length of four and two reactive sites, 2,3-butanediol (2,3-BD) is a versatile building block molecule for the synthesis of both fine and commodity chemicals for use as drugs, cosmetics or liquid fuels. Diacetyl and acetoin have important applications in the food, beverage and fragrance industries. Kristala Jones-Prather and colleagues from the MIT (Cambridge, MA, USA) designed new strains of Escherichia coli that are able to produce acetoin together with small amounts of 2,3-BD. They first designed the metabolic pathways and screened for enzymes of yeast and bacterial origin to perform it. Those have been introduced in E. coli, while bypaths that reduce the yi... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354911 Mon, 08 Mar 2010 00:00:00 +0100 3354911 http://www.medworm.com/index.php?rid=3354910&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213641%26dopt%3DAbstract Authors: Moghaddam AF, Hosseini H no abstract. PMID: 20213641 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354910 Mon, 08 Mar 2010 00:00:00 +0100 3354910 http://www.medworm.com/index.php?rid=3354909&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213642%26dopt%3DAbstract Authors: Biotech news: News on BiofuelsMost read in BTJ: HIV host cell RNAi screening platformNews from industry: Respiratory disease treatment - German biotech sees light at the end of the tunnel in 2010 - Funding photonics - Analyzing tumor cell heterogeneity - Product newsMeeting highlight: Systems Biology of Microorganisms 22-24 March 2010, Institut Pasteur, Paris, FranceNews from academia: How to shoot the messenger - Soybean genome sequence - Canna plant cleans stormwater runoff. PMID: 20213642 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354909 Mon, 08 Mar 2010 00:00:00 +0100 3354909 http://www.medworm.com/index.php?rid=3354908&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213643%26dopt%3DAbstract Authors: Maskow T no abstract. PMID: 20213643 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354908 Mon, 08 Mar 2010 00:00:00 +0100 3354908 http://www.medworm.com/index.php?rid=3354907&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213644%26dopt%3DAbstract Authors: Bignell E no abstract. PMID: 20213644 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354907 Mon, 08 Mar 2010 00:00:00 +0100 3354907 http://www.medworm.com/index.php?rid=3354906&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213645%26dopt%3DAbstract Authors: Dillon SP, D'Souza A, Kurien BT, Scofield RH The authors would like to acknowledge the National Institutes of Health for funding (grant number 5R01AR053734). PMID: 20213645 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354906 Mon, 08 Mar 2010 00:00:00 +0100 3354906 http://www.medworm.com/index.php?rid=3354905&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213646%26dopt%3DAbstract Authors: Laurent V, Fraix A, Montier T, Cammas-Marion S, Ribault C, Benvegnu T, Jaffres PA, Loyer P HepaRG progenitor cells are capable of differentiating into hepatocyte-like cells that express a large set of liver-specific functions. These cells, however, only express small amounts of an important cytochrome P450, the CYP2E1, which limits their use for toxicological studies of drugs metabolized by this pathway. Our aim was to establish an efficient transfection protocol to increase CYP2E1 expression in HepaRG cells. Transfection protocols of the green fluorescent protein (GFP) reporter gene were evaluated using electroporation and cationic lipids belonging to the lipophosphonates, lipophosphoramidates and lipids derived from glycine betaine. Following optimization of the charge ratio... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354905 Mon, 08 Mar 2010 00:00:00 +0100 3354905 http://www.medworm.com/index.php?rid=3354904&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213647%26dopt%3DAbstract Authors: Barth SW, Briviba K, Watzl B, Jäger N, Marko D, Esselen M Irinotecan is an anticancer agent that stabilizes topoisomerase I/DNA complexes. So far, no test system has been reported for directly determining irinotecan-induced stabilization of topoisomerase I/DNA complexes in organs in vivo. We adapted an 'in vivo complexes of enzyme to DNA' (ICE) bioassay to assess irinotecan activity in the stomach, duodenum, colon and liver of male Wistar rats after a single treatment with irinotecan (100 mg/kg body weight, intraperitoneally). This was compared to the control group receiving 0.9% sodium chloride intraperitoneally. In addition, the DNA strand breaking properties of irinotecan were measured in mucosal cells from the distal colon by single-cell gel electrophoresis (comet ass... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354904 Mon, 08 Mar 2010 00:00:00 +0100 3354904 http://www.medworm.com/index.php?rid=3354903&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213648%26dopt%3DAbstract Authors: no abstract. PMID: 20213648 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3354903 Mon, 08 Mar 2010 00:00:00 +0100 3354903 http://www.medworm.com/index.php?rid=3269971&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151445%26dopt%3DAbstract Authors: Palacios F, Cota G, Horjales S, Lima A, Battistoni J, Sotelo-Silveira J, Marín M 'Conformational diseases' are a group of diverse disorders that have been associated with misfolding of specific proteins, leading to their aggregation in particular cell tissues. Despite their relevance, the mechanisms involved in neurodegenerative processes remains poorly understood. Mutations in Cu,Zn superoxide dismutase (SOD1) are implicated in death of motor neurons in amyotrophic lateral sclerosis. Among others, the SOD1(G93A) mutation is known to weaken the structure and this could lead to conformational variations of the protein. As an approach to understand the tissue-specific propensity of protein aggregation, we developed an experimental procedure allowing rapid extraction of vari... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3269971 Thu, 11 Feb 2010 00:00:00 +0100 3269971 http://www.medworm.com/index.php?rid=3269970&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151446%26dopt%3DAbstract Authors: Blazeck J, Alper H The advent of high throughput genome-scale bioinformatics has led to an exponential increase in available cellular system data. Systems metabolic engineering attempts to use data-driven approaches - based on the data collected with high throughput technologies - to identify gene targets and optimize phenotypical properties on a systems level. Current systems metabolic engineering tools are limited for predicting and defining complex phenotypes such as chemical tolerances and other global, multigenic traits. The most pragmatic systems-based tool for metabolic engineering to arise is the in silico genome-scale metabolic reconstruction. This tool has seen wide adoption for modeling cell growth and predicting beneficial gene knockouts, and we examine here how th... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3269970 Thu, 11 Feb 2010 00:00:00 +0100 3269970 http://www.medworm.com/index.php?rid=3269969&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151447%26dopt%3DAbstract Authors: Jungbauer A no abstract. PMID: 20151447 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3269969 Thu, 11 Feb 2010 00:00:00 +0100 3269969 http://www.medworm.com/index.php?rid=3269968&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151448%26dopt%3DAbstract Authors: no abstract. PMID: 20151448 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3269968 Thu, 11 Feb 2010 00:00:00 +0100 3269968 http://www.medworm.com/index.php?rid=3269967&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151449%26dopt%3DAbstract This study demonstrates that plant molecular farming is a viable approach for the bioproduction of human-derived growth factors. TRANSGENIC CROP SAFETY: Herman et al., Biotechnol. J. 2009, 5, 172-182To evalutate the safety of transgenic crops one can analyse the composition of the transgenic crop and compare it to the parent plant or seed. If the composition of nutrients is found to be equivalent to that of non-transgenic variant that is considered safe, then further safety assessment of the transgenic crop can focus solely on the intended modification, like the expression of a transgenic protein. Statistical methods that are used in clinical medicine to compare new generic drugs with brand-name drugs can then be applied to evaluate the equivalence. However, commonly used equivalence limit... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3269967 Thu, 11 Feb 2010 00:00:00 +0100 3269967 http://www.medworm.com/index.php?rid=3269966&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151450%26dopt%3DAbstract Authors: BTJ best cover contestBiotech paper watch:Triple knockout cell lines in one goTurning carbon dioxide into liquid fuelDual malaria/cholera vaccines from chloroplastAutomated single cell clone pickingDisulfide trapping of yeast surface proteinspH changes upon freezing of buffered solutionsIdentifying cancerspecific glycosylationp53, a double-edged sword in the fight against cancerMost read in BTJ:SUMOylation and cell signallingBioengineering news:Biofuels for future generationsTextile wastewater decolorizationEngineering joint cartilage. PMID: 20151450 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3269966 Thu, 11 Feb 2010 00:00:00 +0100 3269966 http://www.medworm.com/index.php?rid=3269965&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151451%26dopt%3DAbstract Authors: Facts and figuresBiotech researchBiodiversityOil resources and climate change. PMID: 20151451 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3269965 Thu, 11 Feb 2010 00:00:00 +0100 3269965 http://www.medworm.com/index.php?rid=3269964&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20151454%26dopt%3DAbstract Authors: no abstract. PMID: 20151454 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3269964 Thu, 11 Feb 2010 00:00:00 +0100 3269964 http://www.medworm.com/index.php?rid=3220907&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20108272%26dopt%3DAbstract Authors: Meng L, Feldman L Extraction of high-quality RNA from Arabidopsis seeds has been a challenge. Here we report a two-step TRIzol-based procedure for RNA extraction from Arabidopsis siliques and dry seeds. This procedure employs a modified, high pH (pH 9.5) extraction buffer. High pH plus the addition of either DTT or beta-mercaptoethanol in the extraction buffer effectively inhibits RNase activity during the extraction, and removes most polysaccharides, polyphenols and other insoluble material. TRIzol reagent was subsequently used to purify the RNA. Using this procedure we isolated high-quality DNA-free RNA samples without DNase I treatment from Arabidopsis seeds or siliques in less than 3 h. PMID: 20108272 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3220907 Wed, 27 Jan 2010 00:00:00 +0100 3220907 http://www.medworm.com/index.php?rid=3220906&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20108273%26dopt%3DAbstract Authors: Jha AK, Prasad K Green, low-cost, and reproducible Lactobacillus-mediated biosynthesis of metal and oxide nanoparticles are reported. Silver and titanium dioxide nanoparticles are synthesized using Lactobacillus sp. procured from yoghurt and probiotic tablets. The synthesis is performed akin to room temperature in the laboratory ambience. X-ray and transmission electron microscopy analyses are performed to ascertain the formation of metallic and oxide nanoparticles. Individual nanoparticles having the dimensions of 10-25 nm (n-Ag) and 10-70 nm (n-TiO(2)) are found. The mechanism involved for the synthesis of metallic and oxide nanoparticles has also been discussed. PMID: 20108273 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3220906 Wed, 27 Jan 2010 00:00:00 +0100 3220906 http://www.medworm.com/index.php?rid=3220905&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20108274%26dopt%3DAbstract In this study, nanoporous gold supercapacitors were produced by electrochemical dealloying of gold-silver alloy. Scanning electron microscopy and energy dispersive X-ray spectroscopy confirmed completion of the dealloying process and generation of a porous gold material with approximately 10 nm diameter pores. Cyclic voltammetry and chronoamperometry of the nanoporous gold electrodes indicated that these materials exhibited supercapacitor behavior. The storage capacity of the electrodes measured by chronoamperometry was approximately 3 mC at 200 mV. Electrochemical storage and voltage-controlled delivery of two model pharmacologic agents, benzylammonium and salicylic acid, was demonstrated. These results suggest that capacitance-based storage and delivery of pharmacologic agents may serve ... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3220905 Wed, 27 Jan 2010 00:00:00 +0100 3220905 http://www.medworm.com/index.php?rid=3220904&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20108275%26dopt%3DAbstract We describe the cloning and characterization of a mutated thermostable DNA polymerase from Thermus aquaticus (Taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step PCR pathogen detection using established real-time detection methods. We demonstrate that this Taq polymerase mutant (Taq M1) has similar PCR sensitivity and nuclease activity as the respective Taq wild-type DNA polymerase. In addition, and in marked contrast to the wild-type, Taq M1 exhibits a significantly increased reverse transcriptase activity especially at high temperatures (>60 degrees C). RNA generally hosts highly stable secondary structure motifs, such as hairpins and G-quadruplexes, which complicate, or in the worst case obviate, reverse transcription (RT). Thus, RT... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3220904 Wed, 27 Jan 2010 00:00:00 +0100 3220904 http://www.medworm.com/index.php?rid=3186425&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20084638%26dopt%3DAbstract Authors: Igbinigie EE, Mutambanengwe CC, Rose PD Fundamental processes involved in the microbial degradation of coal and its derivatives have been well documented. A mutualistic interaction between plant roots and certain microorganisms to aid growth of plants such as Cynodon dactylon (Bermuda grass) on hard coal dumps has recently been suggested. In the present study coal bioconversion activity of nonmycorrhizal fungi was investigated in the C. dactylon/coal rhizosphere. Fungal growth on 2% Duff-agar, gutation formation on nitric acid treated coal and submerged culture activity in nitrogen-rich and -deficient broth formed part of the screening and selection of the fungi. The selected fungal isolates were confirmed to be found in pristine C. dactylon/coal rhizosphere. To simulate bioco... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3186425 Mon, 18 Jan 2010 00:00:00 +0100 3186425 http://www.medworm.com/index.php?rid=3186424&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20084639%26dopt%3DAbstract Authors: Herman RA, Scherer PN, Phillips AM, Storer NP, Krieger M Substantial equivalence has become established as a foundation concept in the safety evaluation of transgenic crops. In the case of a food and feed crop, no single variety is considered the standard for safety or nutrition, so the substantial equivalence of transgenic crops is investigated relative to the array of commercial crop varieties with a history of safe consumption. Although used extensively in clinical medicine to compare new generic drugs with brand-name drugs, equivalence limits are shown to be a poor model for comparing transgenic crops with an array of reference crop varieties. We suggest an alternate model, also analogous to that used in clinical medicine, where reference intervals are constructed for a he... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3186424 Mon, 18 Jan 2010 00:00:00 +0100 3186424 http://www.medworm.com/index.php?rid=3186423&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20084640%26dopt%3DAbstract Authors: Peralta-Yahya PP, Keasling JD The cost-effective production of biofuels from renewable materials will begin to address energy security and climate change concerns. Ethanol, naturally produced by microorganisms, is currently the major biofuel in the transportation sector. However, its low energy content and incompatibility with existing fuel distribution and storage infrastructure limits its economic use in the future. Advanced biofuels, such as long chain alcohols and isoprenoid- and fatty acid-based biofuels, have physical properties that more closely resemble petroleum-derived fuels, and as such are an attractive alternative for the future supplementation or replacement of petroleum-derived fuels. Here, we review recent developments in the engineering of metabolic pathways f... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3186423 Mon, 18 Jan 2010 00:00:00 +0100 3186423 http://www.medworm.com/index.php?rid=3172518&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069576%26dopt%3DAbstract Authors: Cover illustration: Biotech Methods and Advances. The Cover image shows a ferret that is currently used as an animal model for human influenza. Ferrets are extremely susceptible to the infection with human influenza viruses and even develop some of the symptoms seen in humans. In this issue of Biotechnology Journal Krammer et al. describe a new method for the production of swine origin influenza virus like particles that can be used as vaccines (see article on p. 17 - 23 http://dx.doi.org/10.1002/biot.200900267). PMID: 20069576 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172518 Tue, 12 Jan 2010 00:00:00 +0100 3172518 http://www.medworm.com/index.php?rid=3172517&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069577%26dopt%3DAbstract Authors: Patentability of synthetic biology inventions in EuropeThe patent teaching kit. PMID: 20069577 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172517 Tue, 12 Jan 2010 00:00:00 +0100 3172517 http://www.medworm.com/index.php?rid=3172516&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069578%26dopt%3DAbstract Authors: no abstract. PMID: 20069578 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172516 Tue, 12 Jan 2010 00:00:00 +0100 3172516 http://www.medworm.com/index.php?rid=3172515&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069579%26dopt%3DAbstract Authors: News: Plastics from E. coli - Looking again (carefully) at thalidomide - Don't kiss the chimps - Imaging microbial interactions - Probiotics prevent intestinal diseases - Drug resistance in cancer stem cell populations - Triple tag technique turns up tight and loose complexes - It pays to have an immune mother - DNA hybridization mechanisms - Chip-based breast tissue analysis - Nutrient support for engineered tissue - Nano-spheres support axon recovery - Bop-a-hepcidinEnvironmental News: Blueprint for molecular basis of global warming - Environmental biosensors - Predicting the fate of underground carbonJournal Highlights: Focus on Biochips - Neglected tropical diseases - New online portalOpinion: Outwitting evolution: We should pay as much for strategy as technologyMost Read... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172515 Tue, 12 Jan 2010 00:00:00 +0100 3172515 http://www.medworm.com/index.php?rid=3172514&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069580%26dopt%3DAbstract Authors: Jevševar S, Kunstelj M, Porekar VG Since the first PEGylated product was approved by the Food and Drug Administration in 1990, PEGylation has been widely used as a post-production modification methodology for improving biomedical efficacy and physicochemical properties of therapeutic proteins. Applicability and safety of this technology have been proven by use of various PEGylated pharmaceuticals for many years. It is expected that PEGylation, as the most established technology for extension of drug residence in the body, will play an important role in the next generation therapeutics, such as peptides, protein nanobodies and scaffolds, which due to their diminished molecular size need half-life extension. This review focuses on several factors important in the productio... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172514 Tue, 12 Jan 2010 00:00:00 +0100 3172514 http://www.medworm.com/index.php?rid=3172513&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069581%26dopt%3DAbstract Authors: no abstract. PMID: 20069581 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172513 Tue, 12 Jan 2010 00:00:00 +0100 3172513 http://www.medworm.com/index.php?rid=3172512&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069582%26dopt%3DAbstract Authors: Alonso-Gonzalez L, Wagner S, Laible G no abstract. PMID: 20069582 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172512 Tue, 12 Jan 2010 00:00:00 +0100 3172512 http://www.medworm.com/index.php?rid=3172511&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069583%26dopt%3DAbstract Authors: Janssens B, Schaefer U no abstract. PMID: 20069583 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172511 Tue, 12 Jan 2010 00:00:00 +0100 3172511 http://www.medworm.com/index.php?rid=3172510&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069584%26dopt%3DAbstract Authors: INSECT CULTURE BASED SWINE FLU VACCINE: Krammer et al., Biotechnol. J. 2009, 5, 17-23In June 2009 the World Health Organisation (WHO) raised the pandemic level for swine-origin H1N1 influenza A virus to phase 6. Classical egg-derived influenza vaccines may not be produced in time and quantity needed to fight a pandemic. Alternatively, cell culture-based production systems and viruslike particle-based technologies have been established. Grabherr and coworkers from Vienna, Austria, present the production of virus-like particles from swine-origin pandemic H1N1 influenza consisting of hemagglutinin and matrix protein. These were co-expressed in insect cells by the baculovirus expression system. Immunisation of BALB/c mice with these virus-like particles induced high serum antibod... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172510 Tue, 12 Jan 2010 00:00:00 +0100 3172510 http://www.medworm.com/index.php?rid=3172509&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069585%26dopt%3DAbstract Authors: no abstract. PMID: 20069585 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3172509 Tue, 12 Jan 2010 00:00:00 +0100 3172509 http://www.medworm.com/index.php?rid=3135889&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20041443%26dopt%3DAbstract Authors: Krammer F, Nakowitsch S, Messner P, Palmberger D, Ferko B, Grabherr R Recent outbreaks of influenza A highlight the importance of rapid and sufficient supply for pandemic and inter-pandemic vaccines. Classical manufacturing methods for influenza vaccines fail to satisfy this demand. Alternatively, cell culture-based production systems and virus-like particle (VLP)-based technologies have been established. We developed swine-origin pandemic H1N1 influenza VLPs consisting of hemagglutinin (A/California/04/2009) and matrix protein. Hemagglutinin and matrix protein were co-expressed in insect cells by the baculovirus expression system. VLPs were harvested from infection supernatants, purified and used for intraperitoneal immunization of BALB/c mice. Immunization induced high serum... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3135889 Tue, 29 Dec 2009 00:00:00 +0100 3135889 http://www.medworm.com/index.php?rid=3115227&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20024972%26dopt%3DAbstract Authors: Kim Y, Park T One of the major goals of this review was to identify obesity-specific gene profiles in animal models to help comprehend the pathogenic mechanisms and the prediction of the phenotypic outcomes of obesity and its associated metabolic diseases. The genomic examination of insulin-sensitive tissues, such as the adipose and hepatic tissues, has provided a wealth of information about the changes in gene expression in obesity and its associated metabolic diseases. The overexpression of genes related to inflammation, immune response, adhesion molecules, and lipid metabolism is a major characteristic of white adipose tissue, while the overexpression of the genes related to lipid metabolism, adipocyte differentiation, defense, and stress responses is noticeable in the non-... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3115227 Fri, 18 Dec 2009 00:00:00 +0100 3115227 http://www.medworm.com/index.php?rid=3040392&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946874%26dopt%3DAbstract Authors: Schriebl K, Lim S, Choo A, Tscheliessnig A, Jungbauer A The substantial progress in embryonic stem cell (ESC) research could lead to new possibilities in the treatment of various diseases. Currently, applications of ESC for cell therapy are impeded by the presence of potentially teratoma-forming undifferentiated ESC. Thus, a selective and quantitative removal of undifferentiated ESC from a pool of differentiated and undifferentiated cells is essential before cell therapy. We evaluated the highly selective magnetic activated cell sorting (MACS) method for the quantitative removal of undifferentiated ESC. We found that the clearance rates for undifferentiated ESC decreased with decreasing amount of undifferentiated ESC in the cell pool. Using a simplified model calculation we co... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3040392 Fri, 27 Nov 2009 00:00:00 +0100 3040392 http://www.medworm.com/index.php?rid=3040391&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946875%26dopt%3DAbstract Authors: de Felipe P, Luke GA, Brown JD, Ryan MD Where 2A oligopeptide sequences occur within ORFs, the formation of the glycyl-prolyl peptide bond at the C-terminus of (each) 2A does not occur. This property can be used to concatenate sequences encoding several proteins into a single ORF: each component of such an artificial polyprotein is generated as a discrete translation product. 2A and '2A-like' sequences have become widely utilised in biotechnology and biomedicine. Individual proteins may also be co- and post-translationally targeted to a variety of sub-cellular sites. In the case of polyproteins bearing N-terminal signal sequences we observed, however, that the protein downstream of 2A (no signal) was translocated into the endoplasmic reticulum (ER). We interpreted these data a... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3040391 Fri, 27 Nov 2009 00:00:00 +0100 3040391 http://www.medworm.com/index.php?rid=3040390&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946876%26dopt%3DAbstract Authors: Andreou AM, Tavernarakis N SUMOylation is a highly transient post-translational protein modification. Attachment of SUMO to target proteins occurs via a number of specific activating and ligating enzymes that form the SUMO-substrate complex, and other SUMO-specific proteases that cleave the covalent bond, thus leaving both SUMO and target protein free for the next round of modification. SUMO modification has major effects on numerous aspects of substrate function, including subcellular localisation, regulation of their target genes, and interactions with other molecules. The modified SUMO-protein complex is a very transient state, and it thus facilitates rapid response and actions by the cell, when needed. Like phosphorylation, acetylation and ubiquitination, SUMOylation has b... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3040390 Fri, 27 Nov 2009 00:00:00 +0100 3040390 http://www.medworm.com/index.php?rid=3040389&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946877%26dopt%3DAbstract Authors: Schäfer H, Wink M Plants produce a high diversity of natural products or secondary metabolites which are important for the communication of plants with other organisms. A prominent function is the protection against herbivores and/or microbial pathogens. Some natural products are also involved in defence against abiotic stress, e.g. UV-B exposure. Many of the secondary metabolites have interesting biological properties and quite a number are of medicinal importance. Because the production of the valuable natural products, such as the anticancer drugs paclitaxel, vinblastine or camptothecin in plants is a costly process, biotechnological alternatives to produce these alkaloids more economically become increasingly important. This review provides an overview of the state of... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3040389 Fri, 27 Nov 2009 00:00:00 +0100 3040389 http://www.medworm.com/index.php?rid=3040388&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946878%26dopt%3DAbstract Authors: Milne CB, Kim PJ, Eddy JA, Price ND Driven by advancements in high-throughput biological technologies and the growing number of sequenced genomes, the construction of in silico models at the genome scale has provided powerful tools to investigate a vast array of biological systems and applications. Here, we review comprehensively the uses of such models in industrial and medical biotechnology, including biofuel generation, food production, and drug development. While the use of in silico models is still in its early stages for delivering to industry, significant initial successes have been achieved. For the cases presented here, genome-scale models predict engineering strategies to enhance properties of interest in an organism or to inhibit harmful mechanisms of pathogens. Goi... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3040388 Fri, 27 Nov 2009 00:00:00 +0100 3040388 http://www.medworm.com/index.php?rid=3040387&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946879%26dopt%3DAbstract We report a method for the integration of expression cassettes into the Escherichia coli chromosome using rare and dispensable sugar degradation gene loci as sites for integration. Clones carrying successfully recombined DNA fragments in the chromosome are easily screened using a solid differential medium containing the respective sugar compound. As an example for the heterologous expression of a complex natural product biosynthesis pathway, we show the stepwise chromosomal integration of the zeaxanthin biosynthesis pathway from Pantoea ananatis into E. coli. PMID: 19946879 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3040387 Fri, 27 Nov 2009 00:00:00 +0100 3040387 http://www.medworm.com/index.php?rid=3040386&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946880%26dopt%3DAbstract Authors: Sawangwan T, Goedl C, Nidetzky B Compatible solutes constitute a diverse class of low-molecular-mass organic molecules that are accumulated in high intracellular concentrations in response to the external stress of hyperosmolality or high temperature. Many of these compounds like alpha, alpha-trehalose are well known for their stabilizing effect on protein structure and could lead to development of more stable protein formulations. Negatively charged solutes like mannosylglycerate (R-2-O-alpha-D-mannopyranosyl-glycerate) are widespread among (hyper)thermophilic microorganisms and are thought to be exceptionally potent stabilizers of proteins under high-temperature denaturation conditions. To further inquire into the role of compound charge for protective function, we have comp... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3040386 Fri, 27 Nov 2009 00:00:00 +0100 3040386 http://www.medworm.com/index.php?rid=3040385&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946881%26dopt%3DAbstract This article provides possible roles of corosolic acid and hypothetical information on the biosynthetic pathway in plants. PMID: 19946881 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3040385 Fri, 27 Nov 2009 00:00:00 +0100 3040385 http://www.medworm.com/index.php?rid=3040384&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946882%26dopt%3DAbstract Authors: Tuffin M, Anderson D, Heath C, Cowan DA Metagenomics emerged in the late 1990s as a tool for accessing and studying the collective microbial genetic material in the environment. The advent of the technology generated great excitement, as it has provided new opportunities and technologies for studying the wealth of microbial genetic diversity in the environment. Metagenomics has been widely predicted to access new dimensions of protein sequence space. A decade on, we review how far we have actually moved into new sequence space (and other aspects of protein space) using metagenomic tools. While several novel enzyme activities and protein structures have been identified through metagenomic strategies, the greatest advancement has been made in the isolation of novel protein seque... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3040384 Fri, 27 Nov 2009 00:00:00 +0100 3040384 http://www.medworm.com/index.php?rid=3000806&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918779%26dopt%3DAbstract Authors: no abstract. PMID: 19918779 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000806 Fri, 13 Nov 2009 00:00:00 +0100 3000806 http://www.medworm.com/index.php?rid=3000805&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918780%26dopt%3DAbstract Authors: Nobel Prizes 2009: Ribosomes - Telomeres and telomerasesEncyclopaedia of Life Sciences: SNP genotyping technologies - Molecular mimicrySpecial issues: Chinese microbial ecology - Advances in yeast proteomics - MALDI-TOF "Flip-flop" drug susceptibility testNews: Phytophthora infestans genome - Sequencing bacterial transcriptomes - Stem cells from fat - Selecting green clones - Endogenous mutagenic force - Green batteries - Tobacco-produced vaccine - O(2) transport in artificial liver - Endolysins instead of antibiotics - Quick switch key for mitochondria - Climate change shrinks algae - Bacteria degrade microcystinsOpinion: Will biotech banish wrinkles forever?Most ReadSynthetic biologyTips and tricks: Trypsinizing cellsBiotech round the world: KenyaWriting Tips: IMRAD or RAMI... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000805 Fri, 13 Nov 2009 00:00:00 +0100 3000805 http://www.medworm.com/index.php?rid=3000804&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918781%26dopt%3DAbstract Authors: IntroductionSome nanotech/biochip companiesBiochip product newsBiochips in our sister journals. PMID: 19918781 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000804 Fri, 13 Nov 2009 00:00:00 +0100 3000804 http://www.medworm.com/index.php?rid=3000803&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918782%26dopt%3DAbstract Authors: Outram S no abstract. PMID: 19918782 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000803 Fri, 13 Nov 2009 00:00:00 +0100 3000803 http://www.medworm.com/index.php?rid=3000802&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918783%26dopt%3DAbstract Authors: Park HG, Lee SY no abstract. PMID: 19918783 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000802 Fri, 13 Nov 2009 00:00:00 +0100 3000802 http://www.medworm.com/index.php?rid=3000801&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918784%26dopt%3DAbstract Authors: FORENSIC IDENTIFICATION ON CHIPS: Choi and Seo et al., Biotechnol. J. 2009, 4, 1530-1541Short tandem repeat (STR) analysis can be used for genetic fingerprinting of individuals as it is done for forensic human identification. However, the current state-of-the-art STR genotyping processes and instruments are labor intensive, expensive, time consuming, and lack portability. Micro-total-analysis systems or lab-on-a-chip platforms based on microfabrication technologies have the capability to miniaturize and integrate bioanalysis steps in a single format and have already been successfully applied for forensic STR typing. Researchers from Daejeon, Korea, highlight up-to-date work on advanced microdevices for high-throughput STR genotyping, and a portable integrated microsystem for ... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000801 Fri, 13 Nov 2009 00:00:00 +0100 3000801 http://www.medworm.com/index.php?rid=3000800&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918785%26dopt%3DAbstract Authors: Choi KY, Kim TJ, Koh SK, Roh CH, Pandey BP, Lee N, Kim BG The bioconversion of the isoflavonoid daidzein using whole cell Nocardia farcinica IFM10152 showed two kinds of major metabolic modifications, i.e. mono-hydroxylation and subsequent O-methylation. The major hydroxylated products of daidzein prior to the O-methylation reaction were 3',4',7-trihydroxyisoflavone (3'-ODI), 4',6,7-trihydroxyisoflavone (6-ODI) and 4',7,8-trihydroxyisoflavone (8-ODI), which are mono-hydroxylated at the ortho position of each hydroxyl group of daidzein. To identify monooxygenases playing a key role in the monohydroxylation of the A-ring of daidzein, all genes of 27 cytochrome P450s from N. farcinica IFM10152 were cloned and transformed into a E. coli BL21 (DE3) host system. By this enzymatic re... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000800 Fri, 13 Nov 2009 00:00:00 +0100 3000800 http://www.medworm.com/index.php?rid=3000799&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918786%26dopt%3DAbstract Authors: Maynard JA, Lindquist NC, Sutherland JN, Lesuffleur A, Warrington AE, Rodriguez M, Oh SH Technologies based on surface plasmon resonance (SPR) have allowed rapid, label-free characterization of protein-protein and protein-small molecule interactions. SPR has become the gold standard in industrial and academic settings, in which the interaction between a pair of soluble binding partners is characterized in detail or a library of molecules is screened for binding against a single soluble protein. In spite of these successes, SPR is only beginning to be adapted to the needs of membrane-bound proteins which are difficult to study in situ but represent promising targets for drug and biomarker development. Existing technologies, such as BIAcoreTM, have been adapted for membrane prot... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000799 Fri, 13 Nov 2009 00:00:00 +0100 3000799 http://www.medworm.com/index.php?rid=3000798&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918787%26dopt%3DAbstract Authors: Park JW, Kim HJ, Byun JH, Ryu HR, Jeon NL Neurons, one of the most polarized types of cells, are typically composed of cell bodies (soma), dendrites, and axons. Many events such as electric signal transmission, axonal transport, and local protein synthesis occur in the axon, so that a method for isolating axons from somata and dendrites is required for systematically investigating these axonal events. Based on a previously developed neuron culture method for isolating and directing the growth of central nervous system axons without introducing neutrophins, we report three modified microfluidic platforms: (1) for performing biochemical analysis of the pure axonal fraction, (2) for culturing tissue explants, and (3) a design that allows high content assay on same group of cells.... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000798 Fri, 13 Nov 2009 00:00:00 +0100 3000798 http://www.medworm.com/index.php?rid=3000797&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918788%26dopt%3DAbstract Authors: no abstract. PMID: 19918788 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=3000797 Fri, 13 Nov 2009 00:00:00 +0100 3000797 http://www.medworm.com/index.php?rid=2985417&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19902460%26dopt%3DAbstract Authors: Akpor OB, Momba MN The relationship between protozoan biomass concentration and phosphate and nitrate removal was investigated in mixed liquor using three different carbon sources as supplements. The study was carried out using three respective initial biomass concentrations in a shaking flask environment. Samples were taken every 24 h to determine phosphate, nitrate, dissolved oxygen and chemical oxygen demand. The results revealed a direct relationship between decreases in nutrient concentrations and increases in cell densities of the isolates. Between 24 and 96 h, the increases in the protozoan density corresponded to a phosphate decreases from initial ranges of 55.42-57.36 mg/L, 50.27-51.17 mg/L and 50.01-50.83 mg/L to final ranges of 2.46-11.90 mg/L, 0.61-11.80 mg/L and 1... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2985417 Mon, 09 Nov 2009 00:00:00 +0100 2985417 http://www.medworm.com/index.php?rid=2985416&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19902461%26dopt%3DAbstract Authors: Roselin LS, Lin MS, Lin PH, Chang Y, Chen WY Isothermal titration calorimeters (ITCs) are thermodynamic instruments used for the determination of enthalpy changes in any physical/chemical reaction. This can be applied in various fields of biotechnology. This review explains ITC applications, especially in bioseparation, drug development and cell metabolism. In liquid chromatography, the separation/purification of specific proteins or polypeptides in a mixture is usually achieved by varying the adsorption affinities of the different proteins/polypeptides for the adsorbent under different mobile-phase conditions and temperatures. Using ITC analysis, the binding mechanism of proteins with adsorbent solid material is derived by elucidating enthalpy and entropy changes, which offer... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2985416 Mon, 09 Nov 2009 00:00:00 +0100 2985416 http://www.medworm.com/index.php?rid=2918562&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844911%26dopt%3DAbstract Authors: Della Donna L, Ronci M, Sacchetta P, Di Ilio C, Biolatti B, Federici G, Nebbia C, Urbani A Performance enhancing agents (PEAs) are illegally used in cattle and other meat producing species to increase food conversion and lean meat production. Due to the very short breeding cycle, veal calves represent the meat producing bovine category mostly subjected to illicit treatments. These chemical agents are difficult to detect by conventional analytical approaches due to the employment of synergistic formulations at very low dosage and given the use of uncharacterized novel compounds. Such a scenario has fostered a strong interest in the discovery of functional molecular biomarkers for the detection of growth promoting agents in meat producing species. A multivariate MALDI-TOF-MS pro... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2918562 Mon, 19 Oct 2009 23:00:00 +0100 2918562 http://www.medworm.com/index.php?rid=2918561&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844912%26dopt%3DAbstract Authors: Erlendsson LS, Muench MO, Hellman U, Hrafnkelsdóttir SM, Jonsson A, Balmer Y, Mäntylä E, Orvar BL Biologically active recombinant human Flt3 ligand was expressed and isolated from transgenic barley seeds. Its expression is controlled by a tissue specific promoter that confines accumulation of the recombinant protein to the endosperm tissue of the seed. The recombinant Flt3 ligand variant expressed in the seeds contains an HQ-tag for affinity purification on immobilized metal ion affinity chromatography (IMAC) resin. The tagged protein was purified from seed extracts to near homogeneity using sequential chromatography on IMAC affinity resin and cation exchange resin. We also show that the recombinant Flt3 ligand protein undergoes posttranslational modifications: ... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2918561 Mon, 19 Oct 2009 23:00:00 +0100 2918561 http://www.medworm.com/index.php?rid=2918560&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844913%26dopt%3DAbstract Authors: Chaves S, Neto M, Tenreiro R Microbial symbiosis is a ubiquitous aspect of life and was a major element in the ability of insects to explore several adverse environments. To date, the study of symbiosis in insects has been impaired by the unculturability of most symbionts. However, some molecular methods represent powerful tools to help understand insect-microorganism associations and to disclose new symbiont-host systems. Beyond playing an essential role in nutrition and development of the insects, symbionts can produce bioactive compounds that protect the host against adverse environmental conditions, predators and/or direct competitors. Since the search for natural bioactive products and new enzymes is a developing area, understanding the diversity and nature of symbiont-ho... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2918560 Mon, 19 Oct 2009 23:00:00 +0100 2918560 http://www.medworm.com/index.php?rid=2918559&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844914%26dopt%3DAbstract Authors: Choi JY, Seo TS Short tandem repeat (STR) analysis provides genetic fingerprinting of individuals, and is considered as a powerful and indispensable technique for forensic human identification. However, the current state-of-the-art STR genotyping processes and instruments are labor intensive, expensive, time consuming, and lack portability. Micro-total-analysis systems or lab-on-a-chip platforms based on microfabrication technologies have the capability to miniaturize and integrate bioanalysis steps in a single format. Recent progress in microsystems has demonstrated their successful performance for the forensic STR typing with a reduced cost, high speed, and improved high throughput. The purpose of this review article is to highlight up-to-date work on advanced microdevices f... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2918559 Mon, 19 Oct 2009 23:00:00 +0100 2918559 http://www.medworm.com/index.php?rid=2918558&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844915%26dopt%3DAbstract Authors: Tang WL, Zhao H Industrial biotechnology involves the use of enzymes and microorganisms to produce value-added chemicals from renewable sources. Because of its association with reduced energy consumption, greenhouse gas emissions, and waste generation, industrial biotechnology is a rapidly growing field. Here we highlight a variety of important tools for industrial biotechnology, including protein engineering, metabolic engineering, synthetic biology, systems biology, and downstream processing. In addition, we show how these tools have been successfully applied in several case studies, including the production of 1, 3-propanediol, lactic acid, and biofuels. It is expected that industrial biotechnology will be increasingly adopted by chemical, pharmaceutical, food, and agricult... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2918558 Mon, 19 Oct 2009 23:00:00 +0100 2918558 http://www.medworm.com/index.php?rid=2918557&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844916%26dopt%3DAbstract Authors: Jha AK, Prasad K, Prasad K A low-cost green and reproducible microbe (Lactobacillus sp.)-mediated biosynthesis of Sb(2)O(3) nanoparticles is reported. The synthesis was performed at around room temperature. X-ray and transmission electron microscopy analyses were performed to ascertain the formation of Sb(2)O(3) nanoparticles. X-ray analysis indicated that Sb(2)O(3) nanoparticles had a face-centered cubic unit cell structure. Individual nanoparticles as well as a few aggregates of 3-12 nm were found. A possible mechanism for the synthesis of nano Sb(2)O(3) is proposed. PMID: 19844916 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2918557 Mon, 19 Oct 2009 23:00:00 +0100 2918557 http://www.medworm.com/index.php?rid=2918556&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844917%26dopt%3DAbstract Authors: Zimmermann H, Gerhard D, Dingermann T, Hothorn LA Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models. PMID: 19844917 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2918556 Mon, 19 Oct 2009 23:00:00 +0100 2918556 http://www.medworm.com/index.php?rid=2918555&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844918%26dopt%3DAbstract Authors: Pathak A, Patnaik S, Gupta KC Nucleic acids-based next generation biopharmaceuticals (i.e., pDNA, oligonucleotides, short interfering RNA) are potential pioneering materials to cope with various incurable diseases. However, several biological barriers present a challenge for efficient gene delivery. On the other hand, developments in nanotechnology now offer numerous non-viral vectors that have been fabricated and found capable of transmitting the biopharmaceuticals into the cell and even into specific subcellular compartments like mitochondria. This overview illustrates cellular barriers and current status of non-viral gene vectors, i.e., lipoplexes, liposomes, polyplexes, and nanoparticles, to relocate therapeutic DNA-based nanomedicine into the target cell. Despite the awes... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2918555 Mon, 19 Oct 2009 23:00:00 +0100 2918555 http://www.medworm.com/index.php?rid=2918554&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19844919%26dopt%3DAbstract Authors: Sethi D, Gandhi RP, Kuma P, Gupta KC The development of oligonucleotide-based microarrays (biochips) is a major thrust area in the rapidly growing biotechnology industry, which encompasses a diverse range of research areas including genomics, proteomics, computational biology, and pharmaceuticals, among other activities. Microarray experiments have proved to be unique in offering cost-effective and efficient analysis at the genomic level. In the last few years, biochips have gained increasing acceptance in the study of genetic and cellular processes. As the increase in experimental throughput has posed many challenges to the research community, considerable progress has been made in the advancement of microarray technology. In this review, chemical strategies for immobilizatio... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2918554 Mon, 19 Oct 2009 23:00:00 +0100 2918554 http://www.medworm.com/index.php?rid=2902127&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830707%26dopt%3DAbstract Authors: Cover illustrationFocus on synthetic biology. This special issue is edited by Kristala Jones Prather and Alfonso Jaramillo and focuses on computational methods, modeling and applications of synthetic biology. Bottom right: Penicillum simplicissimum in solid-state fermentation producing lipases (2000x magnification by Dr. Ulysses Lins, Microbiology Institute-UFRJ, Brazil), see article by Gutarra et al. on p. 1450 of this issue (http://dx.doi.org/10.1002/biot.200800298). Other images: circuit board and gears (both (c) Getty images). PMID: 19830707 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902127 Tue, 13 Oct 2009 23:00:00 +0100 2902127 http://www.medworm.com/index.php?rid=2902126&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830708%26dopt%3DAbstract Authors: Prather KL, Jaramillo A no abstract. PMID: 19830708 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902126 Tue, 13 Oct 2009 23:00:00 +0100 2902126 http://www.medworm.com/index.php?rid=2902125&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830709%26dopt%3DAbstract Authors: SYNTHETIC BIOLOGY - COMPUTATIONAL METHODS: Kaznessis et al., Biotechnol. J. 2009, 4, 1392-1405Professor Yiannis Kaznessis from Minnesota (MN, USA) discusses how theoretical synthetic biology may liberate empiricism in biological sciences beyond the unaided human brain. Because synthetic biological systems are relatively small and largely independent of evolutionary contexts, they can be represented with mathematical models strongly founded on first principles of molecular biology and laws of statistical thermodynamics. This may then be plausibly used to explain in unambiguous, quantitative terms how biological phenotypic complexity emerges as a result of well-defined biomolecular interactions. He presents SynBioSS, a publicly available software package which implements this m... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902125 Tue, 13 Oct 2009 23:00:00 +0100 2902125 http://www.medworm.com/index.php?rid=2902124&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830710%26dopt%3DAbstract Authors: no abstract. PMID: 19830710 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902124 Tue, 13 Oct 2009 23:00:00 +0100 2902124 http://www.medworm.com/index.php?rid=2902123&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830711%26dopt%3DAbstract Authors: Special focus: Synthetic biologyWhat is synthetic biology?SynBERC - The Synthetic Biology Engineering Research CenterArs SyntheticaiGEM - The International Genetically Engineered Machine competitionSome synthetic biology companiesPaper watch: Synthetic biologyBuilding blocks for novel functionsKnowledge-making distinctions in synthetic biologyScaffold design and manufacturing: From concept to clinicPeptidomimetics - a versatile route to biologically active compoundsMetabolic engineering of E. coliE. coli needs safety valvesSystems-level metabolic engineeringMammalian synthetic biologyChemical aspects of synthetic biologySynthesis of DNA fragments in yeastSynthetic biology and patentable subject matterPatenting artificial life?Metabolic effects of synthetic rewiringEngineering... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902123 Tue, 13 Oct 2009 23:00:00 +0100 2902123 http://www.medworm.com/index.php?rid=2902122&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830712%26dopt%3DAbstract Authors: Facts and figuresAgriculture and green biotechnology in KenyaJomo Kenyatta University of Agriculture and TechnologyRed biotechnology in Kenya. PMID: 19830712 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902122 Tue, 13 Oct 2009 23:00:00 +0100 2902122 http://www.medworm.com/index.php?rid=2902121&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830713%26dopt%3DAbstract Authors: Diez MS, Lam CM, Leprince A, Martins Dos Santos VA no abstract. PMID: 19830713 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902121 Tue, 13 Oct 2009 23:00:00 +0100 2902121 http://www.medworm.com/index.php?rid=2902120&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830714%26dopt%3DAbstract Authors: Kaznessis YN We discuss how a theoretical synthetic biology research programme may liberate empiricism in biological sciences beyond the unaided human brain. Because synthetic biological systems are relatively small and largely independent of evolutionary contexts, they can be represented with mathematical models strongly founded on first principles of molecular biology and laws of statistical thermodynamics. A universal mathematical formalism for describing synthetic constructs may then be plausibly used to explain in unambiguous, quantitative terms how biological phenotypic complexity emerges as a result of well-defined biomolecular interactions. SynBioSS, a publicly available software package, is described that implements this mathematical formalism. PMID: 19830714 [Pub... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902120 Tue, 13 Oct 2009 23:00:00 +0100 2902120 http://www.medworm.com/index.php?rid=2902119&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830715%26dopt%3DAbstract Authors: Marner WD Synthetic biology can be defined as the "repurposing and redesign of biological systems for novel purposes or applications, " and the field lies at the interface of several biological research areas. This broad definition can be taken to include a variety of investigative endeavors, and successful design of new biological paradigms requires integration of many scientific disciplines including (but not limited to) protein engineering, metabolic engineering, genomics, structural biology, chemical biology, systems biology, and bioinformatics. This review focuses on recent applications of synthetic biology principles in three areas: (i) the construction of artificial biomolecules and biomaterials; (ii) the synthesis of both fine and bulk chemicals (including biofuels); a... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902119 Tue, 13 Oct 2009 23:00:00 +0100 2902119 http://www.medworm.com/index.php?rid=2902118&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830716%26dopt%3DAbstract Authors: Lee JY, Jang YS, Lee J, Papoutsakis ET, Lee SY To improve butanol selectivity, Clostridium acetobutylicum M5(pIMP1E1AB) was constructed by adhE1-ctfAB complementation of C. acetobutylicum M5, a derivative strain of C. acetobutylicum ATCC 824, which does not produce solvents due to the lack of megaplasmid pSOL1. The gene products of adhE1-ctfAB catalyze the formation of acetoacetate and ethanol/butanol with acid re-assimilation in solventogenesis. Effects of the adhE1-ctfAB complementation of M5 were studied by batch fermentations under various pH and glucose concentrations, and by flux balance analysis using a genome-scale metabolic model for this organism. The metabolically engineered M5(pIMP1E1AB) strain was able to produce 154 mM butanol with 9.9 mM acetone at pH 5.5, resul... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902118 Tue, 13 Oct 2009 23:00:00 +0100 2902118 http://www.medworm.com/index.php?rid=2902117&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830717%26dopt%3DAbstract Authors: Yao S, Rana S, Liu D, Wise GE Electroporation is a simple and versatile approach for DNA transfer but needs to be optimized for specific cells. We conducted square wave electroporation experiments for rat dental follicle cells under various conditions. These experiments indicated that the optimal electroporation electric field strength was 375 V/cm, and that plasmid concentrations greater than 0.18 mug/muL were required to achieve high transfection efficiency. BSA or fetal bovine serum in the pulsing buffer significantly improved cell survival and increased the number of transfected cells. The optimal pulsing duration was in the range of 45-120 ms at 375 V/cm. This electroporation protocol can be used to deliver DNA into dental follicle cells to study the roles of candidate ge... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902117 Tue, 13 Oct 2009 23:00:00 +0100 2902117 http://www.medworm.com/index.php?rid=2902116&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830718%26dopt%3DAbstract Authors: no abstract. PMID: 19830718 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2902116 Tue, 13 Oct 2009 23:00:00 +0100 2902116 http://www.medworm.com/index.php?rid=2895403&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19824019%26dopt%3DAbstract Authors: Vinci B, Cavallone D, Vozzi G, Mazzei D, Domenici C, Brunetto M, Ahluwalia A Hepatocyte function on 3-D microfabricated polymer scaffolds realised with the pressure-activated microsyringe was tested under static and dynamic conditions. The dynamic cell culture was obtained using the multicompartment modular bioreactor system. Hepatocyte cell density, glucose consumption, and albumin secretion rate were measured daily over a week. Cells seeded on scaffolds showed an increase in cell density compared with monolayer controls. Moreover, in dynamic culture, cell metabolic function increased three times in comparison with static monolayer cultures. These results suggest that cell density and cell-cell interactions are mediated by the architecture of the substrate, while the endogeno... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2895403 Sun, 11 Oct 2009 23:00:00 +0100 2895403 http://www.medworm.com/index.php?rid=2895402&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19824020%26dopt%3DAbstract Authors: Dos Santos AS, Lemos MA, Pereira CA, Jorge SA The aim of this study was to achieve expression of recombinant rabies virus glycoprotein (rRVGP) in Drosophila S2 cells. For this, a cDNA coding for the selection hygromycin antibiotic and the cDNA encoding the RVGP protein under the control of the constitutive actin promoter (Ac) were cloned in an expression plasmid, which was transfected into S2 cells. S2 cell populations (S2AcRVGPHy) showed rRVGP expression in cell lysates, attaining concentrations up to 1.5 mug/10(7) cells (705 mug/L). Of the transfected cells, 20% were shown to express the rRVGP. Cell subpopulations selected by limiting dilution expressed higher rRVGP yields and 90% of the cells were shown to express the rRVGP. Cell populations re-selected by addition of hygro... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2895402 Sun, 11 Oct 2009 23:00:00 +0100 2895402 http://www.medworm.com/index.php?rid=2895401&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19824021%26dopt%3DAbstract Authors: Wu XH, Yu HL, Ba ZY, Chen JY, Sun HG, Han BZ To select an appropriate sampling method for comparison of metabolite profiles between planktonic and biofilm Staphylococcus aureus using NMR techniques, we evaluated three methods: quenching-centrifugation (QC), filtration-quenching (FQ) and filtration-quenching-lyophilization (FQL). We found differences in metabolite loss, yield, reproducibility and metabolite profile. QC caused severe metabolite leakage and possible decomposition of nucleotides. FQ achieved high yields and reproducibility, although it had the disadvantages of long filtration and rinse times before quenching. FQL resulted in a loss of a few metabolites and a lower yield due to lyophilization. Although the biomarkers discovered by each method were nearly the same a... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2895401 Sun, 11 Oct 2009 23:00:00 +0100 2895401 http://www.medworm.com/index.php?rid=2831504&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777475%26dopt%3DAbstract Authors: no abstract. PMID: 19777475 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831504 Tue, 22 Sep 2009 23:00:00 +0100 2831504 http://www.medworm.com/index.php?rid=2831503&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777476%26dopt%3DAbstract Authors: News: Fighting iron deficiency - Measuring cellular forces - Better photobioreactor design for hydrogen production - Artificial nerve cell - After GFP: Infrared IFP - Predictable transfection - RNAi for improved protein production - 3D protein map - Self-irrigating rhubarb - Cool plasma to fight biofilms - Pus elasticity - Plants surviving the frostSpecial Issues: Imaging host-pathogen interactions - Plant in vitro technologies - BiodieselWIREs Nanomedicine and Nanobiotechnology: Drug-eluting stent coatings - Particle fabrication for diagnostic imaging and drug deliveryEncyclopaedia of Life Sciences: Antiprotozoan drugs - Autoimmune disease: TreatmentOpinion: A Korean vision on Green GrowthMost ReadMetagenomicsTips and Tricks: Increase your protein solubility - Printing viabl... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831503 Tue, 22 Sep 2009 23:00:00 +0100 2831503 http://www.medworm.com/index.php?rid=2831502&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777477%26dopt%3DAbstract Authors: Cover illustration: Methods and Advances in Biotech. Cover images depict tools for metabolite analysis in biocatalytic synthesis (see p. 1253). Direct infusion mass spectrometry and LC-MS (bottom) have been particularly useful for the rapid analysis of complex metabolites, derivatives and homologues. The complementary separation power of capillary electrophoresis (middle) combined with sensitive detection is very useful for the analysis of charged metabolites. Chromatographic techniques like GC, LC and TLC are the true workhorses for all classes of metabolites and advanced separations using high-performance liquid chromatography instruments (top) with adequate metabolite detection push the limits even further. Images courtesy from R. Wohlgemuth. PMID: 19777477 [PubMed - a... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831502 Tue, 22 Sep 2009 23:00:00 +0100 2831502 http://www.medworm.com/index.php?rid=2831501&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777478%26dopt%3DAbstract Authors: Lee SY no abstract. PMID: 19777478 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831501 Tue, 22 Sep 2009 23:00:00 +0100 2831501 http://www.medworm.com/index.php?rid=2831499&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777479%26dopt%3DAbstract Authors: USING PLANT HORMONES IN BIOTECHNOLOGY: Csukasi et al., Biotechnol. J. 2009, 4, 1293-130Plant hormones have determinant roles in the final value of agricultural products since plant domestication. Therefore, advances in deciphering their structure, synthesis, perception and signaling pathways have been a main focus for research. Application of basic information on plant hormones in food quality and production has established some landmarks in plant biotechnology, e.g. from the dramatic increase in rice yield to long life tomatoes. The review presented by Victoriano Valpuesta and colleagues from Málaga, Spain, compiles all these success events in plant biotechnology when hormones are involved. In addition, future targets for genetic improvement are suggested after summariz... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831499 Tue, 22 Sep 2009 23:00:00 +0100 2831499 http://www.medworm.com/index.php?rid=2831498&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777480%26dopt%3DAbstract Authors: Biotech news and views:Up-scaled vaccine productionBiodiesel synthesis by biotechnological methodsBiological particles in ice cloudsMicroorganisms producing nanoparticlesNanotechnology boost: MIT-INLBook highlight:Systems biology and biotechnology of Escherichia coliMost accessed articles in BTJ:Bacteria biosensorsTomography of malaria parasitesNew scaffold for artificial skinNew on the market:Millipore's disposable bioreactorOlympus' all-in-one microscope familyEngineering in Life Sciences:Increasing your yieldsBiosensing ethylene dibromideModeling cellulose formationBioEssays highlights:SNPing the RNAi connectionStem, but not equalFolding metal. PMID: 19777480 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831498 Tue, 22 Sep 2009 23:00:00 +0100 2831498 http://www.medworm.com/index.php?rid=2831497&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777481%26dopt%3DAbstract Authors: no abstract. PMID: 19777481 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831497 Tue, 22 Sep 2009 23:00:00 +0100 2831497 http://www.medworm.com/index.php?rid=2831496&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777482%26dopt%3DAbstract Authors: Facts and FiguresRegulation of research in AustraliaGreen biotechnologySome Australian biotech companies. PMID: 19777482 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831496 Tue, 22 Sep 2009 23:00:00 +0100 2831496 http://www.medworm.com/index.php?rid=2831495&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777483%26dopt%3DAbstract Authors: Wohlgemuth R Metabolic networks have been an interesting starting point not only for the design of synthetic routes in a similar sequence of reactions, e.g., in biomimetic syntheses, but also for assembling a number of biocatalytic steps by preparing the required enzymes and auxiliary reagents. Retrosynthetic analysis involving multiple biocatalytic reactions steps therefore needs to consider the practically realized biocatalytic single steps. The opportunities for route selection are enlarged if novel synthetic reactions connecting easily available starting materials and products are found, and/or both biocatalytic and classical reactions of organic chemistry are utilized. Tools and ingredients for biocatalytic synthesis are of special interest for reactions difficult to achi... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831495 Tue, 22 Sep 2009 23:00:00 +0100 2831495 http://www.medworm.com/index.php?rid=2831494&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777484%26dopt%3DAbstract Authors: Kasper C no abstract. PMID: 19777484 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831494 Tue, 22 Sep 2009 23:00:00 +0100 2831494 http://www.medworm.com/index.php?rid=2831493&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19777485%26dopt%3DAbstract Authors: no abstract. PMID: 19777485 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2831493 Tue, 22 Sep 2009 23:00:00 +0100 2831493 http://www.medworm.com/index.php?rid=2804917&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19757497%26dopt%3DAbstract Authors: Zhu D, Hua L Transaminases catalyze amino transfer reactions from amino donors such as amino acids or amines to keto acids or ketones to give chiral amino acid or amines in optically pure form. alpha-Amino acid dehydrogenases catalyze the asymmetric reductive amination of alpha-keto acids using ammonia as amino donor to furnish L-amino acids. The distinct features and synthetic application of these two enzymes are reviewed in an effort to illustrate their promising and challenging aspects in serving as approaches to the direct asymmetric synthesis of optically pure amines from the corresponding keto compounds, a formidable problem in organic chemistry. PMID: 19757497 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2804917 Tue, 15 Sep 2009 23:00:00 +0100 2804917 http://www.medworm.com/index.php?rid=2766994&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19728340%26dopt%3DAbstract Authors: Freimark D, Jèrôme V, Freitag R The identification of highly expressing clones is a crucial step in the development of cell lines for production of recombinant proteins. Here we present a method based on the co-expression of enhanced green fluorescent protein (EGFP) that allows clonal selection in standard 96-well cell culture plates. The genes encoding the EGFP protein and the protein of interest are linked by an internal ribosome entry site and thus are transcribed into the same mRNA but are translated independently. Since both proteins arise from a common mRNA, the EGFP expression level correlates with the expression level of the therapeutic protein for each clone. By expressing recombinant growth factors in CHO cells, we demonstrate the robustness and performance... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2766994 Tue, 01 Sep 2009 23:00:00 +0100 2766994 http://www.medworm.com/index.php?rid=2713276&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685455%26dopt%3DAbstract Authors: Cover illustration: Biotech in the financial crisis. This special issue of Biotechnology Journal covers how the current financial turmoil creates problems but also chances for biotech. Forum articles and biotech highlights ask whether biotech can rescue economy and environment (green growth). Image (c) PhotoDisc, Inc. PMID: 19685455 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713276 Thu, 13 Aug 2009 23:00:00 +0100 2713276 http://www.medworm.com/index.php?rid=2713275&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685456%26dopt%3DAbstract Authors: Lee SY no abstract. PMID: 19685456 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713275 Thu, 13 Aug 2009 23:00:00 +0100 2713275 http://www.medworm.com/index.php?rid=2713274&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685457%26dopt%3DAbstract Authors: Jungbauer A no abstract. PMID: 19685457 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713274 Thu, 13 Aug 2009 23:00:00 +0100 2713274 http://www.medworm.com/index.php?rid=2713273&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685458%26dopt%3DAbstract This article by Mikhail Rabinovich from Moscow, Russia, discusses several aspects of how white biotechnology, and especially the biofuel industry, is affected by the current financial situation. THE BIOTECHNOLOGY INDUSTRY: WHAT'S NEXT?: Guertin, Biotechnol. J. 2009, 4, 1124-1131The current financial crisis is not only affecting banks and car manufacturers worldwide. It also has a profound impact on other key areas of the economy including biotechnology. A new era for biotechnology that will be more and more influenced by financial, political, demographical and ecological issues has begun. Risk and money are most probably the two most important factors which rule biotechnology industries. Especially because developing drugs is certainly among the riskiest businesses as only one product out ... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713273 Thu, 13 Aug 2009 23:00:00 +0100 2713273 http://www.medworm.com/index.php?rid=2713272&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685459%26dopt%3DAbstract Authors: Pierce J no abstract. PMID: 19685459 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713272 Thu, 13 Aug 2009 23:00:00 +0100 2713272 http://www.medworm.com/index.php?rid=2713271&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685460%26dopt%3DAbstract Authors: Gruber AC no abstract. PMID: 19685460 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713271 Thu, 13 Aug 2009 23:00:00 +0100 2713271 http://www.medworm.com/index.php?rid=2713270&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685461%26dopt%3DAbstract Authors: BEYOND BORDERS: THE GLOBAL BIOTECHNOLOGY REPORT 2009 BY ERNST & YOUNG: New pathways to sustainabilityKey financial resultsKey regional findingsThe path ahead: beyond business models as usualOutlook SPECIAL PAPER WATCH: BIOTECH IN THE FINANCIAL CRISIS: The financial crisis and academic researchFinancial order and world politics: crisis, change and continuityFrom crisis to opportunity: Environmental jolt, corporate acquisitions, and firm performanceWhat caused the financial crisis?Trust, confidence, and the 2008 global financial crisisRegulatory hurdles for transgenic biofuel cropsCompanies rehearse a very different future: Connecting leadership capability and strategy execution through simulationLife sciences roundtable: Strategy and financingAcademic entrepreneurship. ... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713270 Thu, 13 Aug 2009 23:00:00 +0100 2713270 http://www.medworm.com/index.php?rid=2713269&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685462%26dopt%3DAbstract Authors: no abstract. PMID: 19685462 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713269 Thu, 13 Aug 2009 23:00:00 +0100 2713269 http://www.medworm.com/index.php?rid=2713268&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685463%26dopt%3DAbstract Authors: no abstract. PMID: 19685463 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713268 Thu, 13 Aug 2009 23:00:00 +0100 2713268 http://www.medworm.com/index.php?rid=2713267&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685464%26dopt%3DAbstract Authors: Favicchio R no abstract. PMID: 19685464 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713267 Thu, 13 Aug 2009 23:00:00 +0100 2713267 http://www.medworm.com/index.php?rid=2713266&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685465%26dopt%3DAbstract Authors: Rabinovich M no abstract. PMID: 19685465 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713266 Thu, 13 Aug 2009 23:00:00 +0100 2713266 http://www.medworm.com/index.php?rid=2713265&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685466%26dopt%3DAbstract Authors: Guertin PA no abstract. PMID: 19685466 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713265 Thu, 13 Aug 2009 23:00:00 +0100 2713265 http://www.medworm.com/index.php?rid=2713264&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685467%26dopt%3DAbstract Authors: Kewell B, Webster A no abstract. PMID: 19685467 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713264 Thu, 13 Aug 2009 23:00:00 +0100 2713264 http://www.medworm.com/index.php?rid=2713263&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685468%26dopt%3DAbstract Handbook of Biomineralization Strategies and Techniques to Promote Plant Growth. By Edmund Bäuerlein, Peter Behrens, and Matthias Epple (Eds.). Biotechnol J. 2009 Aug 14;4(8):1225-1226 Authors: Kirchner B no abstract. PMID: 19685468 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713263 Thu, 13 Aug 2009 23:00:00 +0100 2713263 http://www.medworm.com/index.php?rid=2713262&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19685472%26dopt%3DAbstract Authors: no abstract. PMID: 19685472 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2713262 Thu, 13 Aug 2009 23:00:00 +0100 2713262 http://www.medworm.com/index.php?rid=2692016&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19670251%26dopt%3DAbstract Authors: Jérôme V, Heider A, Schallon A, Freitag R The study of the non-viral gene delivery process at the molecular level, e.g. during the transfection of mammalian cells, is currently limited by the difficulties of specifically detecting the transfected plasmid DNA within the cells. Here we describe the in vivo production of 5-bromodeoxyuridine (BrdU)-labelled plasmid DNA by a thymine-requiring Escherichia coli strain leading to 92 +/- 15% BrdU incorporation while minimizing plasmid structure alteration. The labelled plasmid is produced on the milligram scale in a two-stage cultivation process. The relevance of this approach for plasmid DNA visualisation in the field of gene delivery is demonstrated by localising the BrdU-labelled plasmid DNA via immunodetection/fluorescenc... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2692016 Thu, 06 Aug 2009 23:00:00 +0100 2692016 http://www.medworm.com/index.php?rid=2692015&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19670252%26dopt%3DAbstract Authors: Kubitzki T, Minör D, Mackfeld U, Oldiges M, Noll T, Lütz S Bovine enterokinase is a serine protease that catalyzes the hydrolysis of peptide bonds and plays a key role in mammalian metabolism. Because of its high specificity towards the amino acid sequence (Asp)(4)-Lys, enterokinase is a potential tool for the cleavage of fusion proteins, which are gaining more importance in biopharmaceutical production. A candidate for adaptive cancer immunotherapy is mucin 1, which is produced recombinantly as a fusion protein in CHO cells. Here, we present the first repetitive application of immobilized enterokinase for the cleavage of the mucin fusion protein. The immobilization enables a facile biocatalytic process due to simplified separation of the biocatalyst and the target p... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2692015 Thu, 06 Aug 2009 23:00:00 +0100 2692015 http://www.medworm.com/index.php?rid=2627477&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618384%26dopt%3DAbstract Authors: Kocabaş P, Calık P, Calık G, Ozdamar TH This review focuses on the construction of a global, comprehensive understanding of Bacillus subtilis through microarray studies. The microarray studies in B. subtilis were analysed based on the theme of the work, by mentioning the growth media, bioreactor operation conditions, RNA isolation method, number of data points analysed in exponential or stationary phases, compared genotypes, induction and repression ratios, investigated gene(s) and their positive and/or negative influences. Based on the theme and scope of the studies, the articles were reviewed under seven thematic sections, i.e., effects of gene deletion(s) or overexpression, effects of overexression of heterologous genes, comparison of global gene expressio... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627477 Thu, 16 Jul 2009 23:00:00 +0100 2627477 http://www.medworm.com/index.php?rid=2627476&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618385%26dopt%3DAbstract Authors: Kocabıyık S, Demirok B On page 1064, 5 lines from the bottom, it states "Lys is found at position 461 (Ec umbering) instead of Ala [28, 29]."The correct sentence should be "However, in the E.coli enzyme, instead of Lys at position 461 (Ec numbering), there is Ala-461 [28, 29]." PMID: 19618385 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627476 Thu, 16 Jul 2009 23:00:00 +0100 2627476 http://www.medworm.com/index.php?rid=2627475&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618386%26dopt%3DAbstract Authors: no abstract. PMID: 19618386 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627475 Thu, 16 Jul 2009 23:00:00 +0100 2627475 http://www.medworm.com/index.php?rid=2627474&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618387%26dopt%3DAbstract Authors: Yoo SM no abstract. PMID: 19618387 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627474 Thu, 16 Jul 2009 23:00:00 +0100 2627474 http://www.medworm.com/index.php?rid=2627473&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618388%26dopt%3DAbstract Authors: no abstract. PMID: 19618388 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627473 Thu, 16 Jul 2009 23:00:00 +0100 2627473 http://www.medworm.com/index.php?rid=2627472&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618389%26dopt%3DAbstract Authors: News: Mutagenic biodiesel blends - Technicolor cancer imaging - Anticancer nanoparticle - Increased oxygen transfer in baffled microtiter plates - Transgenic barley growing on acid soil - Brain music - Laser light-induced brain waves - First genome sequence of ruminant speciesSpecial issues: Cytometry of microbes - Food-borne MycotoxinsBook highlights: Biotech funding trends - Biotechnology in Flavor ProductionOpinion: Another biofuel blunder?Tips and tricks: Good to know: Gel ElectrophoresisTest your knowledge. Do you recognize this?Most readWriting tipsBriefs: A hypothetical new model of LDL - Gaden Award - Patenting hES cells in Europe. PMID: 19618389 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627472 Thu, 16 Jul 2009 23:00:00 +0100 2627472 http://www.medworm.com/index.php?rid=2627471&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618390%26dopt%3DAbstract Authors: Cover illustration: Biotech in Turkey. The Sultan Ahmed Mosque is one of the most famous monuments of Turkish and Islamic art. Also known as the "Blue Mosque", it dominates the skyline of Istanbul, the largest city in Turkey and the capital of the Ottoman Empire. This special issue of BTJ, edited by Hikmet Geçkil and Pinar Calik, is devoted to the history, research and developments in biotechnology in Turkey. The Forum magazine and Review articles focus on some of Turkey's 150 research institutions and biotech companies. Articles by Turkish authors cover fermentation processes, microarray technology, biosensors and apoptosis. Image (c) ImageState. PMID: 19618390 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627471 Thu, 16 Jul 2009 23:00:00 +0100 2627471 http://www.medworm.com/index.php?rid=2627470&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618391%26dopt%3DAbstract Authors: Geçkil H, Calık P no abstract. PMID: 19618391 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627470 Thu, 16 Jul 2009 23:00:00 +0100 2627470 http://www.medworm.com/index.php?rid=2627469&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618392%26dopt%3DAbstract Authors: TUBITAK - The scientific and technological research council of TurkeyVISION 2023The Turkish research area (TRA)TUBITAK Marmara Research Center (MAM)Turkish research - Facts and figures. PMID: 19618392 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627469 Thu, 16 Jul 2009 23:00:00 +0100 2627469 http://www.medworm.com/index.php?rid=2627468&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618393%26dopt%3DAbstract This study characterizes a new pro-apoptotic BCL-2 protein that will have an impact in apoptosis of the healthy and malignant gut. PMID: 19618393 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627468 Thu, 16 Jul 2009 23:00:00 +0100 2627468 http://www.medworm.com/index.php?rid=2627467&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618394%26dopt%3DAbstract Authors: Middle East Technical University (METU), AnkaraAnkara UniversityHacettepe University, AnkaraIstanbul Technical UniversityBoğaziçi University, IstanbulInönü University, MalatyaAkdeniz University, Antalya. PMID: 19618394 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627467 Thu, 16 Jul 2009 23:00:00 +0100 2627467 http://www.medworm.com/index.php?rid=2627466&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618395%26dopt%3DAbstract Authors: NüveMetis BiotechnologyPakmayaDr. Zeydanli Life SciencesDepapharmArs Arthro Biotechnology. PMID: 19618395 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627466 Thu, 16 Jul 2009 23:00:00 +0100 2627466 http://www.medworm.com/index.php?rid=2627465&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618396%26dopt%3DAbstract Authors: Turkish material for recombinant protein productionGrowth hormone production in Bacillus subtilisNetwork analysis in yeast signalingFixed proteins on gold surfacesNew software for microarray analysisAntibody purification on nanospheresCLEAN - Environmental news from Turkey. PMID: 19618396 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627465 Thu, 16 Jul 2009 23:00:00 +0100 2627465 http://www.medworm.com/index.php?rid=2627464&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19618397%26dopt%3DAbstract Authors: Ozdamar TH The term biotechnology first appeared in the programs of the Scientific and Technological Research Council of Turkey (TUBITAK) in 1982. The State Planning Organization (SPO) in 1988 defined biotechnology and the scientific fields. Moreover, it put forward an institutional framework and suggested priority areas for research and development. Turkey has been researching and investing in biotechnology for almost four decades. This review covers the development of science and technology policy with its history, consensus and consequences, bio-industries in Turkey, and research activities in biotechnology at Turkish Universities. Details are provided by the research groups in response to a common request for information on their activities and major publications in the fi... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2627464 Thu, 16 Jul 2009 23:00:00 +0100 2627464 http://www.medworm.com/index.php?rid=2611668&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19606429%26dopt%3DAbstract Authors: Gutarra ML, de Godoy MG, Silva JD, Guedes IA, Lins U, Castilho LD, Freire DM A comparative study of Penicillium simplicissimum morphology and lipase production was performed using solid-state (SSF) and submerged (SmF) fermentation. SSF was carried out on babassu cake as culture medium and SmF on a semi-synthetic medium and a medium based on suspended babassu cake grains. Yield of product on biomass, specific activity and conidia production were 3.3-, 1.3- and 2-fold higher in SSF. In SmF, the type of fungus growth differed according to the medium. Using the semi-synthetic medium, the fungus formed densely interwoven mycelial masses without conidia production, whereas using the babassu-based medium the fungus formed free mycelia and adhered to the surfaces of the grains, produc... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2611668 Tue, 14 Jul 2009 23:00:00 +0100 2611668 http://www.medworm.com/index.php?rid=2611667&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19606430%26dopt%3DAbstract Authors: Laible G, Alonso-González L The development of methods for cell-mediated transgenesis, based on somatic cell nuclear transfer, provides a tremendous opportunity to shape the genetic make-up of livestock animals in a much more directed approach than traditional animal breeding and selection schemes. Progress in the site-directed modulation of livestock genomes is currently limited by the low efficiencies of gene targeting imposed by the low frequency of homologous recombination and limited proliferative capacity of primary somatic cells that are used to produce transgenic animals. Here we review the current state of the art in the field, discuss the crucial aspects of the methodology and provide an overview of emerging approaches to increase the efficiency of gene targetin... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2611667 Tue, 14 Jul 2009 23:00:00 +0100 2611667 http://www.medworm.com/index.php?rid=2611666&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19606431%26dopt%3DAbstract In this study, we demonstrate that significant alteration of the fluorogenic dye activation by the active V(H) or V(L) domains can occur by partnering with different V(H) or V(L) complementary domains in the scFv format. Hybrid scFvs can be generated that have fluorogen-activating domains that are completely inhibited by interactions with other domains. Such hybrid scFvs are excellent platforms for the development of several types of genetically encoded, fluorescence-generating biosensors. PMID: 19606431 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2611666 Tue, 14 Jul 2009 23:00:00 +0100 2611666 http://www.medworm.com/index.php?rid=2611665&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19606432%26dopt%3DAbstract Authors: Paco L, Galarneau A, Drone J, Fajula F, Bailly C, Pulvin S, Thomas D Hemoglobin is a member of the hemoprotein superfamily whose main role is to transport O(2) in vertebrate organisms. It has two known promiscuous enzymatic activities, peroxidase and oxygenase. Here we show for the first time that bovine hemoglobin also presents a catalase-like activity characterized by a V(max )of 344 muM/min, a K(M )of 24 mM and a k(cat) equal to 115/min. For high anthracene and hemoglobin concentrations and low hydrogen peroxide concentrations, this activity inhibits the expected oxidation of anthracene, which occurs through a peroxidase-like mechanism. Anthracene belongs to the polycyclic aromatic hydrocarbon (PAH) family whose members are carcinogenic and persistent pollutants found in in... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2611665 Tue, 14 Jul 2009 23:00:00 +0100 2611665 http://www.medworm.com/index.php?rid=2585490&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19585532%26dopt%3DAbstract Authors: Csukasi F, Merchante C, Valpuesta V Plant hormones are signal molecules, present in trace quantities, that act as major regulators of plant growth and development. They are involved in a wide range of processes such as elongation, flowering, root formation and vascular differentiation. For many years, agriculturists have applied hormones to their crops to either increase the yield, or improve the quality of the commercial product. Nowadays, the knowledge of hormone biosynthesis, degradation and signaling pathways has allowed the utilization of biotechnological tools to further improve the main agricultural crops. Natural or artificial mutants, with impaired functioning of the corresponding genes, have been adopted because of their superior phenotype in specific agricultural tr... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2585490 Mon, 06 Jul 2009 23:00:00 +0100 2585490 http://www.medworm.com/index.php?rid=2585489&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19585533%26dopt%3DAbstract Authors: Ullrich R, Liers C, Schimpke S, Hofrichter M Extracellular peroxygenase from the agaric fungus Agrocybe aegerita is a versatile biocatalyst that oxygenates various substrates by means of hydrogen peroxide. The enzyme is routinely produced in suspensions of soybean meal and has until now been purified by several steps of fast protein liquid chromatography (FPLC) using different ion exchangers. The final protein fraction had a molecular mass of 46 kDa but still consisted of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms. This made it difficult to further purify the individual protein forms. Since homogeneous protein fractions are a pre-requisite for X-ray crystallograp... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2585489 Mon, 06 Jul 2009 23:00:00 +0100 2585489 http://www.medworm.com/index.php?rid=2585488&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19585534%26dopt%3DAbstract Authors: Kurt AG, Aytan E, Ozer U, Ates B, Geckil H Given the well-established beneficial effects of Vitreoscilla hemoglobin (VHb) on heterologous organisms, the potential of this protein for the production of L-DOPA and dopamine in two bacteria, Citrobacter freundii and Erwinia herbicola, was investigated. The constructed recombinants bearing the VHb gene (vgb(+)) had substantially higher levels of cytoplasmic L-DOPA (112 mg/L for C. freundii and 97 mg/L for E. herbicola) than their respective hosts (30.4 and 33.8 mg/L) and the vgb(-) control strains (35.6 and 35.8 mg/L). Further, the vgb(+) recombinants of C. freundii and E. herbicola had 20-fold and about two orders of magnitude higher dopamine levels than their hosts, repectively. The activity of tyrosine phenol-lyase, the enzyme c... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2585488 Mon, 06 Jul 2009 23:00:00 +0100 2585488 http://www.medworm.com/index.php?rid=2585487&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19585537%26dopt%3DAbstract Authors: Deshpande KS, Ahamed T, Ter Horst JH, Jansens PJ, van der Wielen LA, Ottens M This paper reviews the basic principles of the recently developed self-interaction chromatography (SIC) technique with regard to protein solution stability and protein crystallization. It gives experimental protocols for both normal-scale and micro-scale SIC experiments and reviews recent developments and current applications of this novel technique in the biopharmaceutical area. This paper aims to be a benchmark in the further proliferation of this highly effective and fast technology for the rational design of stable aqueous formulations of therapeutic proteins and the determination of solution conditions favoring protein crystallization. PMID: 19585537 [PubMed - as supplied by publisher] (Sour... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2585487 Mon, 06 Jul 2009 23:00:00 +0100 2585487 http://www.medworm.com/index.php?rid=2580598&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19579217%26dopt%3DAbstract Authors: Talukdar V, Konar A, Datta A, Choudhury AR no abstract. PMID: 19579217 [PubMed - as supplied by publisher] (Source: Biotechnology Journal) Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2580598 Thu, 02 Jul 2009 23:00:00 +0100 2580598 http://www.medworm.com/index.php?rid=2580597&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19579218%26dopt%3DAbstract Authors: Chatterjee S, Bhattacharjee K, Konar A With the advent of the microarray technology, the field of life science has been greatly revolutionized, since this technique allows the simultaneous monitoring of the expression levels of thousands of genes in a particular organism. However, the statistical analysis of expression data has its own challenges, primarily because of the huge amount of data that is to be dealt with, and also because of the presence of noise, which is almost an inherent characteristic of microarray data. Clustering is one tool used to mine meaningful patterns from microarray data. In this paper, we present a novel method of clustering yeast microarray data, which is robust and yet simple to implement. It identifies the best clusters from a given dataset on the... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2580597 Thu, 02 Jul 2009 23:00:00 +0100 2580597 http://www.medworm.com/index.php?rid=2580596&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19579219%26dopt%3DAbstract Authors: Le Brun V, Friess W, Schultz-Fademrecht T, Muehlau S, Garidel P The purpose of the presented study is to understand the physicochemical properties of proteins in aqueous solutions in order to identify solution conditions with reduced attractive protein-protein interactions, to avoid the formation of protein aggregates and to increase protein solubility. This is assessed by measuring the osmotic second virial coefficient (B(22)), a parameter of solution non-ideality, which is obtained using self-interaction chromatography. The model protein is lysozyme. The influence of various solution conditions on B(22) was investigated: protonation degree, ionic strength, pharmaceutical relevant excipients and combinations thereof. Under acidic solution conditions B(22) is positive, favorin... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2580596 Thu, 02 Jul 2009 23:00:00 +0100 2580596 http://www.medworm.com/index.php?rid=2580595&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19579220%26dopt%3DAbstract Authors: Ozbayraktar FB, Ulgen KO Sphingolipids constitute a biologically active lipid class that is significantly important from both structural and regulatory aspects. The manipulation of sphingolipid metabolism is currently being studied as a novel strategy for cancer therapy. The basics of this therapeutic approach lie in the regulation property of sphingolipids on cellular processes, which are important in a cell's fate, such as cell proliferation, apoptosis, cell cycle arrest, senescence, and inflammation. Furthermore, the mutations in the enzymes catalyzing some specific reactions in the sphingolipid metabolism cause mortal lysosomal storage diseases like Fabry, Gaucher, Niemann-Pick, Farber, Krabbe, and Metachromatic Leukodystrophy. Therefore, the alteration of the sphingolipid... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2580595 Thu, 02 Jul 2009 23:00:00 +0100 2580595 http://www.medworm.com/index.php?rid=2549196&cid=s_37624_70_f&fid=37624&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19557793%26dopt%3DAbstract Authors: Couplan E, Le Cann M, Le Foll C, Corporeau C, Blondel M, Delarue J The phosphatidylinositol 3-kinase (PI3K) pathway controls the regulation of cell growth, proliferation, migration and apoptosis. In many tumors, the PI3K gene is mutated or overexpressed, and/or the PI3K pathway is hyperactive. PI3K is therefore a potential pharmacological target for the development of anti-tumor drugs. Some polyunsaturated fatty acids (PUFA), when given in the diet, may lead to a decrease in PI3K activity. We used a yeast-based model to reconstitute the PI3K/PTEN/Akt pathway to study the effects of long-chain polyunsaturated n-3 fatty acids on PI3K, and found that various PUFA were able to alleviate toxicity induced by overexpression of PI3K. The various PUFA had no significant effect on the s... Biotechnology Journal journals http://www.medworm.com/rss/comments.php?id=2549196 Wed, 24 Jun 2009 23:00:00 +0100 2549196