MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Cancer Cytopathology' source. Sat, 10 Oct 2009 19:29:14 +0100 http://www.medworm.com/index.php?rid=2086582&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.24143 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=2086582 Thu, 08 Jan 2009 05:00:00 +0100 2086582 http://www.medworm.com/index.php?rid=2086583&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.24078 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=2086583 Tue, 06 Jan 2009 05:00:00 +0100 2086583 http://www.medworm.com/index.php?rid=2493865&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.24099 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=2493865 Fri, 19 Dec 2008 00:00:00 +0100 2493865 http://www.medworm.com/index.php?rid=2039292&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23948 In this study, the authors evaluated the applicability of flow-cytometric immunophenotyping (FCI) and compared it with immunocytochemistry (ICC) for the accurate categorization of MSRCTs.In total, 37 consecutive MSRCTs that had been diagnosed with FNA cytology were analyzed by ICC and FCI using a panel of antibodies against desmin, vimentin, CD99/major histocompatibility class I-related antigen 2, neuron-specific enolase, and pancytokeratin. The final diagnoses included Ewing sarcoma (n = 17), rhabdomyosarcoma (n = 6; 4 embryonal and 2 alveolar subtypes), neuroblastoma (n = 10), desmoplastic small round cell tumor (n = 2), and retinoblastoma (n = 2).Accurate categorization was possible in 67.5% of cases by ICC and in 64.8% of cases by FCI. Concordant immunophenotyping results with either t... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=2039292 Wed, 17 Dec 2008 05:00:00 +0100 2039292 http://www.medworm.com/index.php?rid=1968114&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23951 Although liquid-based cervicovaginal cytology has high sensitivity for detecting dysplastic/malignant lesions, many pitfalls exist. Cell blocks can be prepared from residual liquid-based cervicovaginal material and used for immunohistochemistry. The aim of this study was to evaluate a new marker, ProEx C, on cell blocks and its ability to distinguish dysplastic/malignant lesions from morphologically abnormal but benign cells. The results of this study were compared with previously reported results for p16 and Ki-67 on the same material.ProEx C is a cocktail of monoclonal antibodies against proteins associated with aberrant S phase cell cycle induction (topoisomerase IIA, minichromosome maintenance protein 2). ThinPrep (CytycCorp., Boxborough, Mass) cervicovaginal specimens from 79 patients... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1968114 Wed, 19 Nov 2008 05:00:00 +0100 1968114 http://www.medworm.com/index.php?rid=1951245&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23981 The objectives of the current study were to examine GST-pi and P-gp expression in cytologic specimens from patients with unresectable NSCLC and to determine the association of that expression with response to chemotherapy and survival.Patients with unresectable, cytologically diagnosed NSCLC were eligible for the study. Diagnosis was made by fiberoptic bronchoscopy, and staging was done according to international standards. All patients received sequential chemoradiotherapy and were re-evaluated for treatment response. GST-pi and P-gp expression levels were evaluated by immunocytochemistry and immunohistochemistry of bronchial brushing/washing and bronchial tissue biopsy, respectively. Survival was defined as the time between diagnosis and death or last follow-up at 24 months.Thirty-nine p... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1951245 Wed, 12 Nov 2008 05:00:00 +0100 1951245 http://www.medworm.com/index.php?rid=1938229&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23982 In this report, the authors describe a complementary approach to thyroid biopsy that has worked well.All thyroid FNA and CB/CP that were performed in the IR Department during the year 2005 were reviewed. Follow-up histology and records of all procedural complications were retrieved.Seven hundred thirteen thyroid biopsies qualified, 225 biopsies (31%) had FNA with CB/CP (85% had only CP for evaluation), and 488 biopsies (69%) had only FNA. The final unsatisfactory rate in IR for FNA was 8.7%; this was reduced to 3.4% with the use of CB/CP. The addition of CB also helped to obtain a more definitive diagnosis in suboptimal FNA specimens. Cytologic-histologic correlation was comparable for FNA only cases and FNA/CB cases. There were no significant procedural complications in CB cases.FNA in co... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1938229 Fri, 07 Nov 2008 05:00:00 +0100 1938229 http://www.medworm.com/index.php?rid=1938230&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23984 The objective of this study was to compare findings after a cytologic report of low-grade squamous intraepithelial lesion, cannot exclude high-grade squamous intraepithelial lesion (LSIL-H) with findings after a report of low-grade squamous intraepithelial lesion (LSIL).A review of patient records revealed that 312 women had cytologic findings of LSIL-H, and 324 consecutive women in a comparison group had cytologic findings of LSIL during 2005. Findings over 6 months after diagnosis were retrieved and analyzed using chi-square tests, Fisher exact tests, and independent group t tests.Histology was available for 194 of 312 women (64%) with LSIL-H and for 184 of 324 women (57%) with LSIL. Of these, 47 of 194 women (24%) with LSIL-H had grade 2 cervical intraepithelial neoplasia or greater (CI... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1938230 Wed, 05 Nov 2008 05:00:00 +0100 1938230 http://www.medworm.com/index.php?rid=1929800&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23983 The objective of the current study was to evaluate and describe the cytologic features of extragonadal germ cell tumors (GCTs), both primary and metastasis from gonadal sites, in fine-needle aspiration cytology.Aspirates from 88 extragonadal GCTs were retrieved with their clinical features and cytologic smears. The smears were assessed for cellularity, cell patterns, and cytologic features, which were summarized. Histopathology was available in 47 cases, and cytohistologic correlation was done in all such cases.Of 88 cases, 57 with adequate cytologic material were analyzed. Each type of GCT, except for embryonal carcinoma, had characteristic morphologic features. Seminomas had dyscohesive tumor cells with well defined, vacuolated cytoplasm; prominent nucleolus; and background lymphocytes. ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1929800 Tue, 04 Nov 2008 05:00:00 +0100 1929800 http://www.medworm.com/index.php?rid=1929801&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23949 Previous studies have confirmed the low predictive value of a diagnosis of atypical squamous cells, cannot exclude a high-grade squamous intraepithelial lesion (ASC-H) in a Papanicolaou (Pap) smear for subsequent high-grade dysplasia in the postmenopausal age group. It appears plausible that the decrease in estrogen inherent in the postmenopausal state likely produces reactive cytologic atypia, which is misinterpreted as ASC-H. The change in hormone levels observed in pregnant patients, postpartum patients, and contraceptive users, as a corollary, potentially could create a similar diagnostic dilemma. In the current study, the impact of age and altered hormone status on the frequency of ASC-H was assessed to answer the following questions: Is the low predictive value of ASC-H in postmenopa... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1929801 Mon, 03 Nov 2008 05:00:00 +0100 1929801 http://www.medworm.com/index.php?rid=1920727&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23947 The objective of the current study was to compare the results of liquid-based cytology and HPV DNA testing through polymerase chain reaction in 99 penile samples collected from men presenting with condyloma acuminate or male partners of HPV-infected women who had attended a public health service in the city of Belo Horizonte, Minas Gerais, Brazil. Classic and nonclassic cytomorphologic signs were adopted to evaluate the presence of HPV infections in penile smears.HPV DNA was detected in 93 (93.9%) of the 99 samples analyzed. Koilocytosis was detected in 1 smear and nonclassic signs were detected in 23 smears, 22 of which were found to be positive for HPV DNA.The cytopathologic detection of HPV infection in penile samples collected for liquid-based cytology is low, even when cytologic noncl... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1920727 Thu, 30 Oct 2008 04:00:00 +0100 1920727 http://www.medworm.com/index.php?rid=1911114&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23950 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1911114 Tue, 28 Oct 2008 04:00:00 +0100 1911114 http://www.medworm.com/index.php?rid=1802350&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23798 Apart from several subjective criteria, ASCUS and LSIL are defined by nuclear enlargement of 2.5× to 3× and [ge]3× the area of a normal intermediate squamous cell nucleus, respectively. The aim of this study was to assess the ability of observers with various degrees of experience to estimate nuclear area ratios.Forty-five participants (5 anatomic pathologists, 5 cytopathologists, 2 cytopathology fellows, 16 pathology residents, 8 cytotechnologists, 6 medical students, and 3 cytopreparatory staff members) judged the area ratios of pairs of squamous cell nuclei imaged at ×100 objective magnification. For Test 1 (T1), participants chose between 5 preset area ratios (1.0× to 1.4×, 1.5× to 1.9×, 2.0× to 2.4×, 2.5× to 2.9×, 3.0× to 3.4×) for 15 pairs of cells with ratios falling i... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1802350 Thu, 18 Sep 2008 04:00:00 +0100 1802350 http://www.medworm.com/index.php?rid=1802352&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23793 The most common presenting site of extracutaneous disease in mycosis fungoides and Sezary syndrome is the peripheral lymph node. Although fine-needle aspiration biopsy has been shown to be a valuable diagnostic technique in evaluating lymphadenopathy, its utility in patients with cutaneous T-cell lymphoma has not been extensively studied. With fine-needle aspiration biopsy, material can be collected for ancillary diagnostic studies and for morphologic evaluation.The authors report a series of 11 fine-needle aspiration biopsy specimens from 10 mycosis fungoides and Sezary syndrome patients. Flow cytometric immunophenotyping and T-cell receptor gamma chain polymerase chain reaction were performed on fine-needle aspiration biopsy material and correlated with cytologic findings.Seven of 10 pat... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1802352 Wed, 17 Sep 2008 04:00:00 +0100 1802352 http://www.medworm.com/index.php?rid=1802351&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23795 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1802351 Wed, 17 Sep 2008 04:00:00 +0100 1802351 http://www.medworm.com/index.php?rid=1797496&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23797 Tissue microarray allows rapid and efficient evaluation of gene expression at the protein level and of immunochemical markers. To our knowledge, there has been no report of constructing cytology microarray using effusion cell blocks and testing its utility in immunochemical marker validation.A total of 23 malignant effusions (primary tumor of breast [5], GI tract [5], lung [5] and ovary [8]) were used to construct a cytology microarray so that 3 cores of 0.6 mm in diameter were taken from the original cell blocks. Antibodies including AE1/AE3, EMA, and Ki-67 were applied to all cases, and CK7, CK20, TTF-1, WT-1, ER, and PR antibodies were used for selected cases. The cellularity, composition of cells, the staining pattern, and the intensity of each antibody were compared between correspond... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1797496 Wed, 17 Sep 2008 04:00:00 +0100 1797496 http://www.medworm.com/index.php?rid=1700057&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23799 In this study, the CSF findings in patients with ependymal neoplasms are summarized and the cytomorphologic features of ependymoma, including its variants, are illustrated.A search of the pathology databases of 2 medical centers was performed to identify all patients with a histologic diagnosis of ependymoma in whom CSF samples were examined. Slides from CSF samples originally reported as atypical, suspicious, or positive were reviewed and the cytomorphologic features assessed. Follow-up included a review of the medical records and histologic correlation.In all, 177 patients with a diagnosis of ependymoma were identified. Of these, 48 had a total of 94 cytologic preparations of CSF. Positive, suspicious, atypical, negative, and nondiagnostic results were noted in 6.4%, 5.3%, 4.3%, 79.7%, a... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1700057 Wed, 13 Aug 2008 04:00:00 +0100 1700057 http://www.medworm.com/index.php?rid=1682057&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23796 This study evaluated the use of a multicolor fluorescence in situ hybridization (FISH) probe panel specifically designed for chronic lymphocytic leukemia (CLL)/SLL and assessed the association between FISH findings and cytologic diagnosis, proliferation index, and risk of death.FNA specimens from 50 patients (32 men and 18 women; mean age, 57 years [range, 36-77 years]) with histologically confirmed CLL and/or SLL were evaluated in this study for chromosomal abnormalities of 11q22 (ATM), 12, 13q14.3, 13q34.3 (LAMP1), and 17p13.1 (p53) by using a multiprobe FISH kit. One of the 50 cases was excluded because of an insufficient number of cells for FISH analysis. The FISH findings were compared with the cytologic diagnoses (26 SLLs, 12 SLLaccs, and 11 tLCLs), Ki-67 immunostaining, and risk of ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1682057 Wed, 06 Aug 2008 04:00:00 +0100 1682057 http://www.medworm.com/index.php?rid=1682058&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23794 Plasmablastic lymphoma (PBL) is a rare form of non-Hodgkin lymphoma that was once believed to occur primarily in the oral cavity of human immunodeficiency virus-positive individuals. Numerous extraoral sites have also been reported to date. To the authors' knowledge, however, only 3 reports in the literature describe its cytologic features. In the current study, the cytologic findings in 5 additional patients are reported, 3 of whom had concomitant second malignancies. The goal of the current study was to define the cytomorphologic features that may help to distinguish PBL from other mimics.Five cases were identified from the pathology files for which cytology was available. The presence of the following was evaluated: cellularity, plasmablastic cells, background necrosis (BN), single-cell... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1682058 Tue, 05 Aug 2008 04:00:00 +0100 1682058 http://www.medworm.com/index.php?rid=1642199&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23634 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1642199 Tue, 22 Jul 2008 04:00:00 +0100 1642199 http://www.medworm.com/index.php?rid=1605542&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23705 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1605542 Fri, 11 Jul 2008 04:00:00 +0100 1605542 http://www.medworm.com/index.php?rid=1605545&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23637 The objective of this report was to describe the cytopathologic and immunocytochemical features of NETs obtained by endoscopic ultrasound (EUS)-guided fine-needle aspiration (FNA).Forty-eight patients who were diagnosed with pancreatic NETs based on EUS-guided FNA were studied retrospectively (from 2002 to 2007). Clinical data, EUS findings, cytopathologic features, and immunocytochemical stains were reviewed for this study. The final histopathologic diagnosis from each patient also was available for comparison.Forty-eight patients (28 men and 20 women) who ranged in age from 16 years to 86 years were selected and had the following clinical findings: solid or multiple pancreatic masses diagnosed by computed tomography or magnetic resonance imaging studies; simultaneous, suspicious, metasta... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1605545 Thu, 10 Jul 2008 04:00:00 +0100 1605545 http://www.medworm.com/index.php?rid=1605544&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23638 The Bethesda system classifies smears that suggest an underlying cervical intraepithelial neoplasia (CIN) as ASC (atypical squamous cell) smears. ASC smears are subdivided into ASCUS (of undetermined significance) and ASCH (cannot exclude a high-grade lesion). Today the management of ASCUS is a triage with HR-HPV testing and colposcopy is recommended for ASCH. The aim was to conduct a study on ASC smears to determine DNA ploidy measurement for the detection of CIN2+.The link between a suspect DNA ploidy assessed by image cytometry and/or a positive HR-HPV testing was analyzed on 69 ASCUS and 82 ASCH smears, and the presence of CIN2+ within 12 months after ASC diagnosis. The ploidy was suspect in case of aneuploidy, multiploidy, or in the presence of cells with a DNA content >5c or >9c.Ever... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1605544 Thu, 10 Jul 2008 04:00:00 +0100 1605544 http://www.medworm.com/index.php?rid=1605543&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23640 Rapid rescreening (RR) of negative Papanicolaou smears (PS) is used in many countries as a quality-assurance measure. Seeding of abnormal slides has been suggested as a way to increase the sensitivity of this procedure. Since 2004, the authors have carried out RR with seeding before issuing reports. In this article, they describe their experience.Abnormal seeds were sourced from the previous day's high-grade cases, both squamous and glandular. Slides were evaluated for the 'degree of difficulty' (which was defined as the number of fields required to find (fields-to-find [FTF]) the abnormality), relabeled, and redotted to make them indistinguishable from the routine RR work. The number of seeds found/missed, the identity of the screener, the type of seeded abnormality, the degree of difficu... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1605543 Thu, 10 Jul 2008 04:00:00 +0100 1605543 http://www.medworm.com/index.php?rid=1507409&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23636 Endometrial cytology sampling devices for direct uterine sampling have been shown in previous studies to be a reliable and relatively painless method for detecting endometrial lesions. The purpose of the current study was to determine the performance characteristics of endometrial cytology for the detection of malignancy and atypical hyperplasia using liquid-based cytology specimens collected with the Tao brush sampler.Brushings of the endometrial cavity were obtained from 139 hysterectomy specimens before routine histopathologic evaluation. Cytology specimens were fixed in PreservCyt and processed using ThinPrep technology. Cytology diagnoses were classified as nondiagnostic, negative, atypical, or positive for malignancy. Histopathologic findings were used as the gold standard for determ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1507409 Wed, 11 Jun 2008 04:00:00 +0100 1507409 http://www.medworm.com/index.php?rid=1507408&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23639 This report is of a 5-year experience with reflex HPV testing in women with AEC and assessment of the potential role of reflex HPV testing in guiding subsequent colposcopy-directed cervical biopsy/curettage in a large tertiary care hospital setting.All AEC cases cytologically diagnosed from July 2001 to June 2006 were retrieved from the Cleveland Clinic database. The histopathologic diagnoses and the results of HPV testing using the Hybrid Capture 2 (HC-II) method were reviewed. The most severe histopathologic diagnosis was recorded.Of a total 332,470 Papanicolaou (Pap) tests performed, 317 cases of AEC had histopathologic follow-up and reflex testing for high-risk HPV. Histopathologic examination of the 64 HPV-positive AEC cases revealed 18 cases of endocervical adenocarcinoma in situ/ade... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1507408 Wed, 11 Jun 2008 04:00:00 +0100 1507408 http://www.medworm.com/index.php?rid=1507407&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23656 The objective of the current study was to study the prevalence and significance of atypical urine cytology with regard to the effect of instrumentation and prior biopsy.All urine cytology cases seen during a 4-year period (2001-2004) with a diagnosis of atypical urothelial cells (AU) were obtained from the cytopathology computer database. In all cases with available surgical follow-up, the following data were extracted: total number and type of urine specimen, the primary histologic diagnosis, and follow-up histologic diagnosis.In all, 1653 voided and 3502 instrumented urine specimens were examined. A diagnosis of AU was rendered in 115 (6.9%) of the voided urine specimens and in 277 (7.9%) of the instrumented specimens. Follow-up histology was available in 70 cases, including 55 instrumen... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1507407 Wed, 11 Jun 2008 04:00:00 +0100 1507407 http://www.medworm.com/index.php?rid=1448773&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23598 Current guidelines recommend that women with negative Papanicolaou (Pap) test results and no endocervical/transformation zone (EC/TZ) sample return for screening within 12 months. For some women, this represents earlier follow-up than advocated in several routine screening guidelines. Controversy remains with regard to the correlation between sampling of the EC/TZ, Pap test quality, and disease risk assessment.A retrospective study was conducted reviewing the results from 143,438 liquid-based cervical Pap tests performed at a large academic women's hospital between July 2005 and December 2006. Vaginal Pap tests were excluded from the study. Women with any Pap result, women with low-grade squamous intraepithelial lesions (LSILs), and patients with high-grade squamous intraepithelial lesion ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1448773 Sat, 17 May 2008 04:00:00 +0100 1448773 http://www.medworm.com/index.php?rid=1448775&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23595 Solid pseudopapillary neoplasm (SPN) and pancreatic endocrine neoplasm (PEN) are uncommon neoplasms that demonstrate characteristic cytologic features. It is also known that both these tumors may share similar morphologic changes. These features not uncommonly pose significant diagnostic challenge for unsuspecting cytopathologists. In the current study, the authors report that recognition of clear cytoplasmic vacuoles in endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) samples from SPN serves as a useful clue that can distinguish this tumor from PEN.The cytologic features from 5 SPN and 20 PEN cases were evaluated. Both Diff-Quik and Papanicolaou stains from these cases were examined. A Fisher exact test of probability was performed to determine differences in the individual c... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1448775 Fri, 16 May 2008 04:00:00 +0100 1448775 http://www.medworm.com/index.php?rid=1448774&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23596 Sputum is an easily accessible diagnostic material for lung cancer early detection by cytologic and molecular genetic analysis of exfoliated airway epithelial cells. However, the use of sputum is limited by its cellular heterogeneity, which includes >95% macrophages and neutrophils and only about 1% bronchial epithelial cells. We propose to obtain concentrated and purified bronchial epithelial cells to improve early detection of lung cancer in sputum samples.Sputum was collected from patients with stage I nonsmall-cell lung cancer, cancer-free smokers, and healthy nonsmokers. Magnetic-assisted cell sorting (MACS) with anti-CD14 and anti-CD16 antibody beads were used to enrich bronchial epithelial cells by depleting macrophages and neutrophils from sputum. Fluorescence in situ hybridization... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1448774 Fri, 16 May 2008 04:00:00 +0100 1448774 http://www.medworm.com/index.php?rid=1439056&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23597 No Abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1439056 Tue, 13 May 2008 04:00:00 +0100 1439056 http://www.medworm.com/index.php?rid=1416082&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23509 Rapid prescreening (RPS) is an internal quality-control (IQC) method that is used both to reduce errors in the laboratory and to measure the sensitivity of routine screening (RS). Little direct comparison data are available comparing RPS with other more widely used IQC methods.The authors compared the performance of RPS, 10% random review of negative smears (R-10%), and directed rescreening of negative smears based on clinical risk criteria (RCRC) over 1 year in a community clinic setting.In total, 6135 smears were evaluated. The sensitivity of RS alone was 71.3%. RPS detected significantly more (132 cases) false-negative (FN) cases than either R-10% (7 cases) or RCRC (32 cases). RPS significantly improved the overall sensitivity of the laboratory (71.3-92.2%; P = .001); neither R-10% nor ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1416082 Fri, 02 May 2008 04:00:00 +0100 1416082 http://www.medworm.com/index.php?rid=1416081&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23538 The Bethesda 2001 (B2001) classification system raised the threshold for reporting atypical squamous cells. The category of Atypical Squamous Cells of Undetermined Significance (ASCUS)-favor reactive has been eliminated with a recommendation for cytologists to judiciously downgrade those Papanicolaou (Pap) tests that would formerly have been in this category to Negative for Intraepithelial Lesion or Malignancy (NILM). The effect of this change on sensitivity and specificity of the Pap test is not yet known.A total of 535 consecutive SurePath Pap tests interpreted as ASCUS during a period from March 1 through December 31, 2001, with follow-up polymerase chain reaction(PCR)-based human papilloma virus (HPV) testing, were independently reviewed by 3 pathologists and 1 cytotechnologist who rei... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1416081 Fri, 02 May 2008 04:00:00 +0100 1416081 http://www.medworm.com/index.php?rid=1405132&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23579 No Abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1405132 Tue, 29 Apr 2008 04:00:00 +0100 1405132 http://www.medworm.com/index.php?rid=1405135&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23510 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1405135 Mon, 28 Apr 2008 04:00:00 +0100 1405135 http://www.medworm.com/index.php?rid=1405134&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23541 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1405134 Mon, 28 Apr 2008 04:00:00 +0100 1405134 http://www.medworm.com/index.php?rid=1405133&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23496 In this study, the authors conducted a comparative quantitative evaluation of the proliferation markers ProEx C (an aberrant S-phase induction marker, human papillomavirus E6-E7 correlated) and MIB-1 in squamous intraepithelial lesions (SIL) to identify a biomolecular profile informative for the diagnosis of high-grade SIL/cervical intraepithelial neoplasia 3 or greater that was complementary to the morphologic Papanicolaou (Pap) test ("biomolecular Pap test").After the cytologic diagnosis, reflex immunocytochemistry was carried out on 76 unstained SurePath cell samples (20 routine samples that were negative for intraepithelial lesion or malignancy and 56 positive samples that were selected with matching histology). Both a morphometric analysis with a software imaging analysis system and a... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1405133 Mon, 28 Apr 2008 04:00:00 +0100 1405133 http://www.medworm.com/index.php?rid=1394863&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23539 The objective of the current study was to describe the cytologic features of LCNEC in an attempt to distinguish it from other pulmonary carcinomas.A cytohistologic study of 11 surgical lobectomy specimens classified as LCNEC was performed. In all these cases, preoperative fine-needle aspiration cytology (FNAC) material was available for review.The cytologic features of the cases were rather similar, resulting in a repetitive pattern. The majority of smears were hypercellular with numerous single, medium-to-large cells. Naked nuclei were abundant but a variable subset of cells demonstrated evident cytoplasm. Groups were 3-dimensional and of variable size, some of them large. Nuclear pleomorphism, molding, and mitosis were common findings. A necrotic background was evident in 6 cases. In 6 c... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1394863 Thu, 24 Apr 2008 04:00:00 +0100 1394863 http://www.medworm.com/index.php?rid=1392289&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23495 For the current report, the authors examined the characteristic features of morphology and molecular biology of phosphatase and tensin homolog (PTEN), [beta]-catenin, and p53 immunocytochemistry in endometrial carcinoma by using thin-layer cytologic preparations.During a 6-month period, 120 endometrial samples were collected directly by using the Uterobrush, and thin-layer specimens were prepared. Immunocytochemical expression levels of PTEN, [beta]-catenin, and p53 were investigated by using 40 specimens of endometrial carcinoma (EC), and 30 specimens each of proliferative endometrium, secretory endometrium, and atrophic endometrium.For PTEN immunoreactivity, the a cutoff value of 50% PTEN expression appeared to be useful for the correct diagnosis of EC in endometrial cytology. For [beta]... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1392289 Wed, 23 Apr 2008 04:00:00 +0100 1392289 http://www.medworm.com/index.php?rid=1374908&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23497 Workload is extensively regulated and often used as a measure of quality in gynecologic cytology. Whether workload correlates with the sensitivity of screening in gynecologic cytology is not known.The sensitivity of gynecologic cytology screening was measured over an 8-month period using the result of full screening coupled with the results of rapid prescreening. Sensitivity results were then correlated with daily workload volumes and the experience level of individual cytotechnologists.Rapid prescreening had an average sensitivity of 41.9% for atypical squamous cells of undetermined significance (ASCUS) and above. Full screening had a corrected sensitivity of 82.2% for ASCUS and above. Rapid prescreening increased the sensitivity of the laboratory to 89.9%. The sensitivity of full screeni... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1374908 Wed, 16 Apr 2008 04:00:00 +0100 1374908 http://www.medworm.com/index.php?rid=1364651&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23498 Analysis of different tumor markers by reverse transcriptase-polymerase chain reaction (RT-PCR) in fine-needle aspiration samples of thyroid nodules has been studied with the objective of improving the accuracy of the preoperative diagnosis of thyroid lesions. The aim of the current study was to investigate thyroid fine-needle aspiration samples inadequate for RT-PCR analysis and to determine whether there is a correlation between their proportion and the method of sampling used or the greatest dimension of the nodules.A total of 350 fine-needle aspiration samples from patients with thyroid nodules were analyzed. After the aspirate was smeared for conventional cytology, the leftover material in the needle was used for RT-PCR analysis in 1 group of 175 patients. In another group of 175 pati... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1364651 Fri, 11 Apr 2008 04:00:00 +0100 1364651 http://www.medworm.com/index.php?rid=1287363&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23411 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1287363 Sat, 08 Mar 2008 05:00:00 +0100 1287363 http://www.medworm.com/index.php?rid=1287364&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23349 In conclusion, reduction of sample complexity by N-GP capturing allows detection of proteins in the [mu]g to ng/mL range. Pleural effusion is a useful source for biomarker research in lung cancer. Cancer (Cancer Cytopathol) 2008. © 2008 American Cancer Society. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1287364 Fri, 07 Mar 2008 05:00:00 +0100 1287364 http://www.medworm.com/index.php?rid=1262630&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23413 The diagnosis of malignant mesothelioma (MM) in serous effusions is difficult but may be achieved by the application of adjuvant methods.The authors cytologically diagnosed 33 effusions as suspicious or positive for MM cells by using DNA-image cytometry (DNA-ICM), immunocytochemistry and AgNOR analysis. The authors further detected 9p21 deletions by chromosomal fluorescence in situ hybridization (FISH). In addition, 31 cases of metastatic carcinomas and 39 of tumor cell-negative effusions were investigated. All diagnoses were confirmed by histologic and/or clinical follow-up.DNA aneuploidy was found in 71% of MMs, 100% of metastatic carcinomas, and in none of the negative effusions. Calretinin was positive in 100% of MMs, in none of the metastatic carcinomas, and in 94.9% of negative effus... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1262630 Thu, 28 Feb 2008 05:00:00 +0100 1262630 http://www.medworm.com/index.php?rid=1262632&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23367 The objective of this study was to elucidate the usefulness of cytologic specimens for HER-2 FISH testing.Histologic and cytologic specimens from 32 patients with invasive ductal carcinoma of the breast were subjected to comparative analysis of HER-2 status by FISH. FISH was performed by using a PathVysion HER-2 DNA Probe Kit according the manufacturer's instructions. The signal ratios of chromosome enumeration probe 17 (CEP17) and HER-2 were estimated and compared. In 15 cytologic specimens, the distance between signals (HER-2 and CEP17) and the nearest nuclear membrane were measured by using 3-dimensional image analysis and confocal microscopy.Cytologic and histologic FISH results were compared. Signal ratios of HER-2/CEP17 were lower in cytologic specimens from 26 of 32 patients compare... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1262632 Wed, 27 Feb 2008 05:00:00 +0100 1262632 http://www.medworm.com/index.php?rid=1262631&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23412 Fine-needle aspiration cytology (FNAC) has been extensively used for many years in the diagnosis of breast lesions, but its use has gradually been reduced in many screening programs because of its controversial inadequate rate and suboptimal accuracy. In the current study, the authors present the quality performance results of FNAC in the Verona screening program, in which an integrated radiopathologic, real-time assessment of suspicious lesions is operative.All cytohistologic assessments during screening at the Verona center were performed in real time by specialized pathologists and radiologists. FNAC activity performance indicators were evaluated according to the European quality assurance guidelines. The effect of radiologic imaging, the guidance system, and the degree of clinicoradiol... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1262631 Wed, 27 Feb 2008 05:00:00 +0100 1262631 http://www.medworm.com/index.php?rid=1256108&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23366 HPV L1 capsid protein is expressed together with the production of infectious viral particles, but its expression and relation to p16 expression, which has been a surrogate marker for human papilloma virus (HPV) infection in cervix, are little studied in cytology samples. The authors aimed to elucidate the relation between L1 capsid protein and p16 protein expressions in liquid-based samples from uterine cervical lesions.Immunochemical analyses using antibodies against L1 capsid protein and p16 protein were carried out on cytological materials obtained from uterine cervical lesions of low-grade squamous intraepithelial lesions (LSILs), high-grade squamous intraepithelial lesions (HSILs), and squamous cell carcinomas (SCCs).L1 capsid protein was positive in 30% of LSILs and 12% of HSILs, bu... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1256108 Tue, 26 Feb 2008 05:00:00 +0100 1256108 http://www.medworm.com/index.php?rid=1256111&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23343 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1256111 Mon, 25 Feb 2008 05:00:00 +0100 1256111 http://www.medworm.com/index.php?rid=1256110&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23347 In this study, the authors evaluated their team's performance in cytologic diagnosis of lobular carcinoma over 11 years and analyzed the reasons for diagnostic failure.Cytologic findings were analyzed in 555 consecutive fine-needle cytopuncture specimens from women with palpable, invasive lobular carcinoma of the breast. The authors also examined the influence of the cytologist's experience, the clinical tumor size, the histologic subtype, and the histologic grade on diagnostic performance. All negative samples were re-examined, along with all samples that had been obtained during the last year of the study, to refine the morphologic description of lobular carcinoma.Malignancy was diagnosed in 68.8% of specimens overall. The individual pathologists diagnosed malignancy in from 44.4% to 81.... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1256110 Mon, 25 Feb 2008 05:00:00 +0100 1256110 http://www.medworm.com/index.php?rid=1256109&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23348 In this study, the authors explored the value of identifying HPV-related tumors in neck metastases to determine the origin of occult primary head and neck squamous cell carcinoma (HNSCC).Thirty FNA biopsies of neck metastases from patients with HNSCC were recovered from the authors' files from 2004 to 2005. The primary sites included 13 oropharynx, 13 oral cavity, and 4 larynx/hypopharynx. All patients had corresponding tissue samples from the neck mass and the primary carcinoma. The FNA specimens and corresponding tissue samples were classified as either nonkeratinizing SCC (NKSCC) or keratinizing SCC (KSCC). In situ hybridization for HPV (HPV-ISH) was performed using ethanol-fixed, Papanicolaou-stained smears. A positive signal was defined as dark blue or black nuclear dots. Correspondin... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1256109 Mon, 25 Feb 2008 05:00:00 +0100 1256109 http://www.medworm.com/index.php?rid=1243347&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23344 Fine-needle aspiration (FNA) cytology of axillary lymph nodes is a simple, minimally invasive technique that can be used to improve preoperative determination of the status of the axillary lymph nodes in patients with breast cancer, thereby serving as a tool with which to triage patients for sentinel versus full lymph node dissection procedures. The aim of the current study was to determine the sensitivity and specificity of FNA cytology to detect metastatic breast carcinoma in axillary lymph nodes.A total of 115 FNAs of axillary lymph nodes of breast cancer patients with histologic follow-up (subsequent sentinel or full lymph node dissection) were included in the current study. The specificity and sensitivity, as well as the positive and negative predictive values, were calculated.The pos... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1243347 Wed, 20 Feb 2008 05:00:00 +0100 1243347 http://www.medworm.com/index.php?rid=1216358&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23346 Expectant management of serous cystadenoma (SCA) of the pancreas requires an accurate preoperative diagnosis. Previously published cytologic diagnostic sensitivities have ranged widely, from 10% to 100%. In the current study, the authors evaluated the diagnostic sensitivity of endoscopic ultrasound (EUS)-guided fine-needle aspiration biopsy (FNAB) and cross-sectional imaging for SCA.Group I consisted of 21 histologically confirmed SCAs. Group II (n = 7 lesions) lacked histologic confirmation and was defined by EUS findings that were consistent with SCA and a cyst fluid carcinoembryonic antigen (CEA) level (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1216358 Fri, 08 Feb 2008 05:00:00 +0100 1216358 http://www.medworm.com/index.php?rid=1216360&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23342 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1216360 Thu, 07 Feb 2008 05:00:00 +0100 1216360 http://www.medworm.com/index.php?rid=1216359&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23345 Spindle cell melanoma is a morphologic variant of melanoma that can be difficult to diagnose on specimens obtained via fine-needle aspiration (FNA). Published cytology studies concerning this entity were based for the most part on small series. In the current study, a large series of metastatic spindle cell melanoma is described and the diagnostic pitfalls present in FNA samples addressed.The authors retrospectively reviewed the cytologic features of 81 metastatic spindle cell melanoma specimens obtained from 67 patients. Corresponding primary tumors or metastatic tumors taken elsewhere from the same patient were also evaluated.The cytologic smears were mostly cellular and comprised of predominantly spindle tumor cells that frequently formed cohesive fascicles or whorls intermingled with s... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1216359 Thu, 07 Feb 2008 05:00:00 +0100 1216359 http://www.medworm.com/index.php?rid=1169177&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23286 The objective of the current study was to test the hypothesis that the cytologic diagnosis of cancer cells can be enhanced by the technique of hyperspectral imaging (HSI).As a proof of principle, HSI was employed to obtain hyperspectrum from a normal human fibroblast, as well as its telomerase-immortalized and SV40-transformed derivatives. Novel algorithms were developed to differentiate among these cell models based on spectral and spatial differences. Using the same technique with modified algorithms, the authors were able to differentiate among normal and precancerous (low-grade [LG] and high-grade [HG]) cervical cells and squamous cell carcinoma (SCC) on liquid-based Papanicolaou (Pap) test slides.The specificity for identifying normal fibroblast cell type based on spatial and spectral... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1169177 Wed, 23 Jan 2008 05:00:00 +0100 1169177 http://www.medworm.com/index.php?rid=1143301&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23255 P16INK4a (p16) has emerged as a biomarker for the detection of high-risk human papillomavirus (HR-HPV) in Papanicolaou (Pap) smears. Many studies have confirmed a strong correlation between p16 immunohistochemical positivity and high-grade squamous intraepithelial lesions (HSIL) of the cervix. Because p16 is predictive of HR-HPV and HSIL, it seems plausible that p16 could be used as a diagnostic tool to triage atypical squamous cells of undetermined significance (ASCUS) Pap smears. In this way, Pap smears with no p16 staining could be recategorized as negative for intraepithelial lesion or malignancy (NILM) before final case disposition, thus preventing unnecessary and costly follow-up.p16 immunostains were performed on 178 ThinPrep (Cytyc, Marlborough, Mass) Pap smears signed out as ASCUS... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1143301 Fri, 11 Jan 2008 05:00:00 +0100 1143301 http://www.medworm.com/index.php?rid=1134469&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23258 Aberrant promoter methylation of selective tumor suppressor genes has been detected in squamous intraepithelial lesions (SIL) and invasive cervical cancer. Identification of methylation profiles of genes that can distinguish high-grade SIL (HSIL) from low-grade SIL (LSIL), and cytologically negative for intraepithelial lesion or malignancy (NILM) residual liquid-based Papanicolaou (Pap) tests may be potentially useful as an ancillary test for cervical cancer screening.Using real-time quantitative methylation-specific polymerase chain reaction (PCR) (QMSP), the authors analyzed the frequency and relative level of promoter methylation for DAPK1, IGSF4, SPARC, and TFPI2 in biopsy-confirmed HSIL and LSIL, and NILM residual liquid-based Pap tests. The percentage of methylation (%M) for each gen... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1134469 Tue, 08 Jan 2008 05:00:00 +0100 1134469 http://www.medworm.com/index.php?rid=1109660&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23254 Immunohistochemistry is helpful in distinguishing metastatic carcinoma from atypical mesothelial cells; however, it is not useful in differentiating atypical mesothelial cells from malignant mesothelial cells. K homolog domain containing protein overexpressed in cancer (KOC), a member of the insulin-like growth factor mRNA-binding protein (IMP) family, also known as L523S and IMP3, is expressed during embryogenesis and in various malignancies. Using a mouse monoclonal antibody (L523S) against KOC, KOC expression was investigated in malignant tumors and reactive mesothelial cells in serous effusions.Seventy-six cases with paraffin-embedded pleural, pericardial, and peritoneal serous effusion cell blocks including 60 malignant serous effusions (11 malignant pleural mesotheliomas and 49 metas... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1109660 Fri, 21 Dec 2007 05:00:00 +0100 1109660 http://www.medworm.com/index.php?rid=1101008&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23257 The ThinPrep Imaging System (TIS) has been approved by the U.S. Food and Drug Administration for use to decrease the number of false-negative results in ThinPrep (TP) gynecologic specimens and increase cytotechnology productivity. Although the increased detection of squamous abnormalities using the TIS has been well documented, to the authors' knowledge, data regarding the impact of the TIS in the detection of glandular abnormalities is limited. The goal of the current study was to evaluate the effectiveness of the TIS in detecting glandular abnormalities in cervicovaginal specimens.TIS evaluated TP tests with histologic confirmation of adenocarcinoma involving the gynecologic system were included in the current study. Two cytotechnologists independently reviewed the cases for the presence... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1101008 Tue, 18 Dec 2007 05:00:00 +0100 1101008 http://www.medworm.com/index.php?rid=1101011&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23252 Multispectral image analysis is an emerging tool that utilizes both spatial and spectral image information to classify images that can be used for the differentiation between benign versus malignant cells. The aim of the current study was to analyze the ability of this tool in differentiating subtle cytologic differences that cannot be appreciated by the human eye. Herein, the authors used fine-needle aspirations (FNAs) of follicular adenoma (FA) and parathyroid adenoma (PA) as a test case.The Nuance platform was used to collect image stacks that were subsequently analyzed with CRI-MLS software, a neural network-based artificial intelligence system that can classify images using automatically "learned" spatial-spectral features. CRI-MLS was trained on random, well-preserved FA cells and PA... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1101011 Mon, 17 Dec 2007 05:00:00 +0100 1101011 http://www.medworm.com/index.php?rid=1101010&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23253 Missed papillary thyroid carcinoma (PTC) diagnoses on fine-needle aspiration (FNA) can result from many causes. To the authors' knowledge, the issue of whether the detection of PTC is correlated with nodule heterogeneity has not been studied to date.The authors identified all thyroidectomy specimens with a diagnosis of PTC that had undergone at least 1 prior FNA in the study institution between 1998 and 2003. The tumor size at the time of the resection, the ultrasound (US)-determined nodule size, and other parameters were compared between the 2 groups in which PTC was or was not diagnosed on FNA.Of a total of 89 specimens, 47 were diagnosed on FNA with an average tumor size of 1.7 cm and an US-determined nodule size of 2.1 cm (a difference of 0.4 cm). Forty-two specimens with a smaller ave... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1101010 Mon, 17 Dec 2007 05:00:00 +0100 1101010 http://www.medworm.com/index.php?rid=1101009&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23256 The objective of the current study was to analyze the cytologic findings after this novel procedure.Isthmic and vaginal Papanicolaou-stained ThinPrep cytology specimens taken from patients after radical vaginal and abdominal trachelectomy were reviewed. The specimens were also analyzed for the presence of benign endocervical cells, lower uterine segment glandular cells, endometrial stromal cells, and endometrial cells. The findings were correlated with the original diagnosis and follow-up, which included subsequent cytology specimens and biopsies.Cytology specimens (n = 223) from 44 patients were included in this study. An endometrial component was identified in 131 of the cases (59%). Twenty-eight cases were diagnosed as abnormal in the original cytology examination. Twenty of these cases... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1101009 Mon, 17 Dec 2007 05:00:00 +0100 1101009 http://www.medworm.com/index.php?rid=1021899&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23116 The diagnostic evaluation of patients with thyroid nodules is imprecise. Despite the benefits of fine-needle aspiration (FNA), most patients who are referred for surgery because of abnormal cytology prove to have benign disease. Recent technologic and procedural advances suggest that this shortcoming can be mitigated, although few data confirm this benefit in unselected patients.A total of 2587 sequential patients were evaluated by thyroid ultrasound and were offered ultrasound-guided FNA (UG-FNA) of all thyroid nodules that measured [ge]1 cm during a 10-year period. Results of aspiration cytology were correlated with histologic findings. The prevalence of thyroid cancer in all patients and in those who underwent surgery was determined. Surgical risk was calculated.Tumors that measured [ge... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=1021899 Tue, 13 Nov 2007 05:00:00 +0100 1021899 http://www.medworm.com/index.php?rid=996386&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23118 The use of high-risk human papillomavirus (HRHPV) testing in the triage of women with a cytologic diagnosis of atypical squamous cells of undetermined significance (ASC-US) has gained widespread acceptance. To date, very little has been reported on the significance of the detection of HRHPV in elderly women.Results of HRHPV testing performed on women aged [ge]50 years were examined for a 20-month period. Reflex testing for HRHPV was performed on residual liquid-based cytology specimens from women who were diagnosed with ASC-US by using the Digene Hybrid Capture method. Follow-up information on women who had HRHPV detected was obtained from subsequent pathology reports (cytology and surgical).HRHPV testing was performed on 762 specimens from women aged [ge]50 years; virus was detected in 10... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=996386 Thu, 01 Nov 2007 04:00:00 +0100 996386 http://www.medworm.com/index.php?rid=989262&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23154 In conclusion, the current federally mandated proficiency test cannot reliably measure the level of expertise of cytologists and, thus, cannot assure that only adequately skilled individuals evaluate Papanicolaou test samples. To render the test suitable for its intended purpose, the authors believe that complete redesign of the test, with the participation of experts in modern test theory, would be advisable. Cancer (Cancer Cytopathol) 2007. © 2007 American Cancer Society. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=989262 Tue, 30 Oct 2007 04:00:00 +0100 989262 http://www.medworm.com/index.php?rid=989264&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23153 No Abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=989264 Mon, 29 Oct 2007 04:00:00 +0100 989264 http://www.medworm.com/index.php?rid=989263&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23117 Despite the historic belief that cytologic screening offers little protection against cervical adenocarcinoma (CAC), there is emerging evidence that, by detecting the precursor lesion, adenocarcinoma in situ (AIS), cervical screening may reduce the incidence of CAC as it has for cervical squamous carcinoma. Because liquid-based cytology is fast replacing the conventional Papanicolaou smear (PS), it is important to establish that it is at least as effective in detecting AIS.The authors calculated the sensitivities of PS and ThinPrep (TP) for 100 women with histologic AIS (from 160 PS slides and 60 TP slides), for 94 women with AIS+high-grade squamous intraepithelial lesion (HSIL) (from 151 PS slides and 50 TP slides), and for 558 women with HSIL (from 788 PS slides and 383 TP slides). All s... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=989263 Mon, 29 Oct 2007 04:00:00 +0100 989263 http://www.medworm.com/index.php?rid=983168&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23119 Bladder cancer is among the 5 most common malignancies worldwide. Patients with bladder cancer are closely followed with periodic cystoscopies and urine cytology analyses due to the significant risk of tumor recurrence. The UroVysion fluorescence in situ hybridization (FISH) test demonstrated higher sensitivity over urine cytology in detecting bladder cancer by most comparative studies.In the current study, the diagnostic usefulness of a combined cytology and FISH analysis approach was tested using the Duet automatic scanning system in patients with benign urine cytology who were being monitored for recurrent urothelial carcinoma or being assessed for various urologic symptoms.By combining the benefits of conventional cytology with molecular diagnostics, a more sensitive detection of bladd... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=983168 Sat, 27 Oct 2007 04:00:00 +0100 983168 http://www.medworm.com/index.php?rid=957110&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23120 The cytomorphology of anaplastic large cell lymphoma (ALCL) is distinctive yet variable. To the authors' knowledge, to date only small case series have described the cytologic findings noted in patients with ALCL. The current series is the largest case series presented to date to retrospectively review the cytomorpholgic findings noted in patients with ALCL, with specific attention paid to those with anaplastic lymphoma kinase (ALK)-negative ALCL.Over a 13-year period, the available Diff-Quik cytology smears and surgical excision specimens taken from patients with ALCL were evaluated. Different clinical and morphologic parameters were evaluated, including ALK status.A total of 37 cases were retrieved and evaluated, 19 of which had both cytology and surgical pathology specimens available fo... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=957110 Wed, 17 Oct 2007 04:00:00 +0100 957110 http://www.medworm.com/index.php?rid=957111&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23122 To the authors' knowledge, few studies exist demonstrating the reliability of fine-needle aspiration (FNA) biopsy for high-grade sarcoma (HGS).In the current study, the authors reviewed their cytopathology database (March 2001 through January 2007) and identified all FNA cases diagnosed as HGS. They also searched their tissue database for all HGS cases that had prior FNA biopsy findings.A total of 107 FNA samples from 98 patients (age range, 13-90 years, with a male:female ratio of 1:1) had an FNA diagnosis of HGS, or had HGS and a prior FNA diagnosis of another entity. Ten cases were nondiagnostic. Of the 97 remaining samples, 6 were diagnosed as something other than HGS (sensitivity of 94%). The positive predictive value of an FNA diagnosis of HGS was 97% (88 of 91 cases). Fifty-four cas... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=957111 Tue, 16 Oct 2007 04:00:00 +0100 957111 http://www.medworm.com/index.php?rid=945236&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23121 Management guidelines for women with Papanicolaou (Pap) test interpretations of ASC-H (atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion) reflect substantial risk, which ranges from 10% to 68%, of a cervical intraepithelial neoplasia grade 2 or worse (CIN2+) in their follow-up histologic samples. The present study was initiated to determine the number of colposcopic procedures and the time frame that are typically required for a definitive diagnosis of a CIN2+ lesion after a Papanicolaou (Pap) test interpretation of ASC-H in routine practice.Clinicopathologic data on consecutive ASC-H interpretations, 97% of which were on liquid-based preparations, were reviewed. The number of biopsies (which was used in this context as a surrogate indicator for the number ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=945236 Fri, 12 Oct 2007 04:00:00 +0100 945236 http://www.medworm.com/index.php?rid=939619&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.23123 No Abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=939619 Wed, 10 Oct 2007 04:00:00 +0100 939619 http://www.medworm.com/index.php?rid=848236&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22947 The objective of this study was to investigate whether the presence of vaginal Candida or dysbacteriosis predisposes women to an increased susceptibility for (pre)neoplasia over time.A retrospective, longitudinal, cohort study was performed and was conducted in a population of 100,605 women, each of whom had 2 smears taken over a period of 12 years as part of the Dutch Cervical Screening Program. From these women, a cohort of 1439 women with Candida and a cohort of 5302 women with dysbacteriosis were selected as 2 separate study groups. The control cohort consisted of women who had completely normal cervical smears (n = 87,903 women). These groups were followed retrospectively over time. The odds ratios (OR) for squamous abnormalities in the follow-up smear for the women in these 3 cohorts... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=848236 Fri, 07 Sep 2007 04:00:00 +0100 848236 http://www.medworm.com/index.php?rid=833221&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22954 This study explores the value of GPC3 expression in liver fine-needle aspirates (FNAs) by immunocytochemistry (ICC), and compares its sensitivity and staining intensity with that of IHC.Archival cytologic material in hepatic FNAs from 20 patients with HCC, 20 patients with metastatic tumors, and 20 patients with benign lesions, were studied. Correlating surgical specimens and/or cell blocks were available for GPC-3 IHC in 16 patients with HCC. All slides were stained with GPC3-1G12 antibody with appropriate positive and negative controls. Staining intensity was graded as 0, no staining; 1, weak cytoplasmic staining; 2, moderate cytoplasmic staining; 3, strong cytoplasmic staining with membranous accentuation. Grades 0 and 1 were regarded as negative; grades 2 and 3 were considered positive... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=833221 Fri, 31 Aug 2007 04:00:00 +0100 833221 http://www.medworm.com/index.php?rid=825307&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22953 The objective of this study was to evaluate whether liquid-based cytology (LBC) can improve high-standard cervical cancer screening cytology further. The primary endpoint was histopathologic high-grade lesions in current and subsequent screening rounds. The secondary endpoints were cytologic diagnosis and inadequate samples.Women were randomized to smear taking by conventional Papanicolaou (Pap) smear or LBC according to the time of appointment. Eight thousand eight hundred ten conventional Pap smears and 4674 LBC samples were included. Evaluations of atypical cytology and referral to colposcopy and treatment were performed as routine procedures. Histopathologic diagnoses were retrieved from a regional database 8 months after the study was closed. The mean follow-up was 2 years and 9 month... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=825307 Tue, 28 Aug 2007 04:00:00 +0100 825307 http://www.medworm.com/index.php?rid=825309&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22946 In this study, the utility of this assay was tested for the detection of HPV in liquid-based cervical cytology specimens.A total of 195 liquid-based cytology specimens were analyzed using the HPV-CARD assay. The results from the assay were compared with HPV polymerase chain reaction (PCR) and typing results. The number of HPV-infected cells and the staining pattern was correlated with the cytology classification.A 91% concordance between HPV-CARD and PCR was observed for the detection of high-risk HPV viruses. A 78% concordance was observed for specimens that were negative for HPV. In ASCUS, low-grade squamous intraepithelial lesion (LSIL), and high-grade squamous intraepithelial lesion (HSIL) categories, the average number of HPV-positive cells per slide was 19 cells, 127 cells, and 450 c... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=825309 Mon, 27 Aug 2007 04:00:00 +0100 825309 http://www.medworm.com/index.php?rid=825308&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22952 In this study, the authors investigated the occurrence and causes of abnormal cervicovaginal cytologies with negative HPV testing.The study was performed on 4130 liquid-based cytology (LBC) specimens from 2918 women who had abnormal cervical cytology or colposcopy or a history of abnormal cervical smear. HPV testing with Hybrid Capture II was repeated in LBC specimens with obviously atypical cytology (squamous intraepithelial lesion or abnormal squamous cells, cannot exclude high-grade lesion) and initial negative test results. The results were compared with the follow-up results and with the cytologic features of the specimens.Of 4130 LBC specimens, 94 (2.28%) showed obviously abnormal cells despite negative HPV results, and 50 of those 94 samples (53.19%) were HPV-positive on a repeated ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=825308 Mon, 27 Aug 2007 04:00:00 +0100 825308 http://www.medworm.com/index.php?rid=819422&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22950 Detecting recurrent bladder carcinoma early is important because it is a multifocal disease that may affect the bladder mucosa, ureters, urethra, and renal pelvis and is associated with high morbidity and mortality rates. However, specimens from patients who have undergone cystectomy with urinary diversion can be difficult to interpret by cytologic evaluation because they often contain degenerated epithelial cells, histiocytes, acute inflammatory cells, bacteria, and cellular debris. In this retrospective study, the reliability of quantitative digital cytometry (QDC) in conjunction with cytologic evaluation in detecting recurrent disease was determined in these patients.In all, 116 specimens were identified from the cytology files from 83 patients who had undergone radical cystectomy with ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=819422 Fri, 24 Aug 2007 04:00:00 +0100 819422 http://www.medworm.com/index.php?rid=811744&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22951 In this report, the authors provide the results from a self-developed program between 2 cytology laboratories.Between 4 and 11 SurePath liquid-based cervical cytology samples were selected at each of the 2 participating laboratories each quarter and exchanged without accompanying patient information. Samples were selected to test both positive and negative high-risk HPV DNA results in roughly equivalent numbers. Samples were run with the Hybrid Capture II method using each laboratory's standard procedure. The result obtained was compared with the originating laboratory's result. Correlation was compared on an ongoing basis as a method to assess analytic performance.Over a 3-year period, 12 exchanges took place, constituting 113 total specimens. Overall, there were 9 exchanges of 76 specime... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=811744 Tue, 21 Aug 2007 04:00:00 +0100 811744 http://www.medworm.com/index.php?rid=811745&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22949 The prevalence of high-risk Human Papillomavirus DNA (hrHPV DNA) in women with negative Papanicolaou (Pap) test results provides a measure of residual risk for cervical neoplasia after cytology screening. The purpose of this study was to document the prevalence of hrHPV DNA in several thousand women ages [ge]30 years with negative ThinPrep Imaging System (TIS)-imaged Pap test results in a large academic hospital cytology laboratory.All cytology-negative TIS-imaged ThinPrep Pap tests (TPPT) with hrHPV DNA tests that were performed by the United States Food and Drug Administration (FDA)-approved Hybrid Capture 2 (HC2) method from May 1, 2005 to November 20, 2006 were identified and reviewed. Imaged-negative Pap test slides associated with a positive hrHPV DNA test result were rescreened manu... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=811745 Mon, 20 Aug 2007 04:00:00 +0100 811745 http://www.medworm.com/index.php?rid=785488&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22955 The Papanicolaou Society of Cytopathology recently proposed 6 diagnostic categories for the classification of thyroid fine-needle aspiration (FNA) cytology. Using these categories, the experience with FNA from 2 institutions was studied with emphasis on cytologic-histologic correlation, source of errors, and clinical management.Patient cytology data were retrieved by a retrospective search of thyroid FNA in the institutional databases. Cytologic diagnoses were classified as unsatisfactory, benign, atypical cellular lesion (ACL), follicular neoplasm (FN), suspicious for malignancy, and positive for malignancy. Samples with a histologic discrepancy were re-evaluated, and clinical follow-up information was recorded.Of of 4703 FNA samples, 10.4% were classified as unsatisfactory, 64.6% were cl... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=785488 Tue, 07 Aug 2007 04:00:00 +0100 785488 http://www.medworm.com/index.php?rid=779916&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22948 Extraskeletal myxoid chondrosarcoma (EMC) is a rare soft-tissue sarcoma rarely subjected to cytologic analysis. With the exception of a few small series, the cytology literature of EMC is largely limited to single-case reports. The purpose was to evaluate the cytomorphology of 8 EMC cases as obtained by imprint/scrape cytology and fine-needle aspiration (FNA) biopsy, to review the literature, and to demonstrate the utility of cytogenetic analysis in the diagnosis of EMC.The cytology files were reviewed for all soft-tissue lesions signed out as chondrosarcoma, myxoid sarcoma, and EMC, and the tissue files for any cases of EMC that had corresponding cytopathology. FNA was performed using a standard technique. Scrape preparations were performed from tissue sent fresh to the laboratory for eit... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=779916 Sat, 04 Aug 2007 04:00:00 +0100 779916 http://www.medworm.com/index.php?rid=768484&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22945 Sampling error is a common explanation of noncorrelation in women whose Papanicolaou (Pap) tests show high-grade intraepithelial lesions (HSIL) but whose follow-up cervical biopsies show only cervical intraepithelial neoplasia (CIN) 1, koilocytosis, or reactive/inflammatory changes. The purpose of this study was to demonstrate the validity of sampling error in this setting by determining the proportion of negative colposcopic cervical biopsies in women with HSIL who subsequently undergo cone/loop electrode excision procedure (LEEP) biopsies or repeat cervical biopsies that confirm the diagnosis of high-grade CIN (HGCIN).In all, 368 cases of HSIL were retrieved from the computerized database from January 1, 2003 to December 31, 2005. Follow-up was obtained as part of routine quality assuran... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=768484 Tue, 31 Jul 2007 04:00:00 +0100 768484 http://www.medworm.com/index.php?rid=717830&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22767 Baseline screening for lung cancer of 2968 high-risk men and women utilizing HRCT enrolled in ELCAP (Early Lung Cancer Action Project) was performed between 1993-2002. Among them, 65 people had surgical resection of their screen-diagnosed lung cancer, 53 of them on the basis of a diagnosis of malignancy or atypical bronchioloalveolar proliferation (ABP) on fine needle aspiration (FNA) biopsy at Weill Medical College of Cornell University (WMC) prior to surgery. The authors compared the diagnosis obtained from the FNA with the subsequent diagnosis from the surgical specimen to assess the reliability of a cytologic diagnosis of lung cancer on FNA of these screen-diagnosed lung cancers.The FNA biopsies were performed with a 22-gauge Wescott needle by 1 radiologist (D.Y.), with preliminary on-... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=717830 Fri, 06 Jul 2007 04:00:00 +0100 717830 http://www.medworm.com/index.php?rid=704259&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22770 In this study, the authors evaluated the contribution of each of these reporting categories to the sensitivity and specificity of a liquid-based Papanicolaou test for endometrial carcinoma or hyperplasia.Over the 3-year study period, in the setting of a large, multihospital health care system, the authors analyzed the results from liquid-based Papanicolaou tests that were performed within the 6 months that preceded a histologic diagnosis of endometrial carcinoma or hyperplasia and that were reported according to the 2001 Bethesda System.Two hundred seventy-two women had a histologic diagnosis of endometrial hyperplasia (n = 199) or malignancy (n = 73) within 6 months after a Papanicolaou test. In total, 188,594 Papanicolaou tests (91,385 from women aged >40 years) were interpreted during t... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=704259 Fri, 29 Jun 2007 04:00:00 +0100 704259 http://www.medworm.com/index.php?rid=681458&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22766 The objective of this study was to investigate the potential of virtual microscopy (VM) as an avenue for the delivery of mandatory cytology proficiency tests).Three senior cytotechnologists and 2 board-certified cytopathologists participated in 3 virtual proficiency tests. Each set consisted of 10 ThinPrep slides that were digitized by an Aperio T3 ScanScope. The cytologic diagnoses covered the range of interpretive guidelines provided by the Centers for Medicare and Medicaid Services (CMS). Each cytotechnologist followed the requirement of a primary screener with the cytopathologists utilizing the secondary screener option.Analysis of the diagnostic interpretation of the first proficiency test showed correct classification of 100% of normal and abnormal cells for primary and secondary scr... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=681458 Wed, 20 Jun 2007 04:00:00 +0100 681458 http://www.medworm.com/index.php?rid=671811&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22866 Endoscopic ultrasound-guided fine-needle aspiration biopsy through the esophagus (EUS-FNA) or the bronchial tree (endobronchial ultrasound guided transbronchial needle aspiration [EBUS-TBNA]) may be used to obtain specimens from mediastinal structures. The accuracy of this procedure has been well documented. However, no studies have studied the reproducibility of the pathologic assessment of the aspirated material.A total of 102 slides from EUS-FNA or EBUS-TBNA were assessed 2 times by 4 pathologists who classified each slide to 1 of 5 diagnostic categories and judged if the aspirate came from a lymph node. Between the 2 rounds the criteria to be used in the assessment of the slides were reviewed in a limited education session. The 4 observers had at least 15 years of pathology experience,... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=671811 Sun, 17 Jun 2007 04:00:00 +0100 671811 http://www.medworm.com/index.php?rid=671813&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22768 Difficulties with cytologic diagnoses on fine-needle aspiration cytology (FNAC) of the liver can be overcome by the application of immunocytochemical panels applied on smears. The aim of the current study was to analyze the performance of a panel of monoclonal antibodies to differentiate hepatocellular carcinoma (HCC) from metastatic carcinoma (MC) or regenerative nodules, and to identify the to date unknown primary sites of carcinomas that had metastasized to the liver.In a validating cohort study, 108 FNACs coin lesions in the liver were routinely evaluated applying immunocytochemistry as an ancillary method. All patients had confirmatory histologic and/or clinical follow-up. A total of 23 HCCs were analyzed for the distinction from MC or regenerative nodules applying a panel of HepPar1,... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=671813 Wed, 13 Jun 2007 04:00:00 +0100 671813 http://www.medworm.com/index.php?rid=671812&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22865 The purpose of the current study was to examine the screening histories of women diagnosed with invasive cervical cancer (ICC) in 2000 who had previous Papanicolaou (Pap) smears deemed to be unsatisfactory or with low-grade findings that did not lead to biopsy.A total of 252 Pap smears from 47 women taken between 1992 and 2000 were included in the study; 247 smears were reexamined at the laboratory of origin before the study and all 252 were then reexamined independently by 2 experienced cytotechnicians and 2 cytopathologists.Of the 47 cases of ICC, 35 were squamous cell carcinoma, 10 were adenocarcinoma, and 2 were other types. On reexamination at the laboratory of origin, 24 cases were upgraded and in the study group 27 cases were upgraded to diagnoses requiring biopsy. On reexamination ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=671812 Wed, 13 Jun 2007 04:00:00 +0100 671812 http://www.medworm.com/index.php?rid=671815&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22691 (PEL) is a human herpes virus-8 (HHV-8)-associated and very rare type of lymphoma usually confined to the body cavities and commonly observed in human immunodeficiency virus (HIV)-infected patients. A comparison was made between the cytologic and immunocytochemical features of 4 cases of PEL encountered in the authors' department with those reported to date in the literature.A comprehensive comparison of the cytologic and immunocytochemical features of the 4 cases with those reported in the literature was conducted.Cytologically, the most consistent features of the 4 cases and those in the literature included large cell size, moderate to abundant cytoplasm, a single nucleus in most cells with occasional bi- or multinucleated giant cells, single to multiple prominent nucleoli, and coarse c... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=671815 Wed, 06 Jun 2007 04:00:00 +0100 671815 http://www.medworm.com/index.php?rid=671814&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22769 Fine-needle aspiration cytology (FNAC) of the head and neck region is well accepted as a diagnostic procedure in the adult population. FNAC in the pediatric population is gaining acceptance as clinicians add this technique to the diagnostic armamentarium. An experience with FNAC of the head and neck region in the pediatric population is described from 2 large inner-city hospitals. Eighty-five cases were retrieved from patients age (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=671814 Wed, 06 Jun 2007 04:00:00 +0100 671814 http://www.medworm.com/index.php?rid=631180&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22694 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=631180 Wed, 23 May 2007 04:00:00 +0100 631180 http://www.medworm.com/index.php?rid=628164&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22772 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=628164 Tue, 22 May 2007 04:00:00 +0100 628164 http://www.medworm.com/index.php?rid=628165&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22771 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=628165 Mon, 21 May 2007 04:00:00 +0100 628165 http://www.medworm.com/index.php?rid=609888&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22687 The authors have noted that in cervical cytology specimens from perimenopausal and postmenopausal women, the diagnosis of atypical squamous cells of undetermined significance (ASC-US), as defined in the Bethesda system, is often not associated with a clinically evident lesion on follow-up. Reflex human papillomavirus (HPV) testing provides an opportunity to distinguish cytologic features of significance from those within the spectrum of benign cellular change in this age group.Liquid-based preparations that were diagnosed as ASC-US between January 2003 and July 2005 at Emory University Hospital were identified from the computer files. The results of HPV-DNA testing were recorded. Two hundred four Papanicolaou tests from perimenopausal women (n = 81, 40-49 years) and postmenopausal women (n... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=609888 Thu, 17 May 2007 04:00:00 +0100 609888 http://www.medworm.com/index.php?rid=609892&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22686 There is an unmet clinical need for economic, minimally invasive procedures that use a limited number of cells for the molecular profiling of tumors in individual patients. Reverse-phase protein microarray (RPPM) technology has been applied successfully to the quantitative analysis of breast, ovarian, prostate, and colorectal cancers using frozen surgical specimens.For this report, the authors investigated the novel use of RPPM technology for the analysis of both archival cytology aspirate smears and frozen fine-needle aspiration (FNA) samples. RPPMs were printed with 63 breast FNA samples that were obtained before, during, and after treatment from 21 patients who were enrolled in a Phase II trial of neoadjuvant capecitabine and docetaxel therapy for breast cancer.Based on an MCF7 cell lin... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=609892 Tue, 08 May 2007 04:00:00 +0100 609892 http://www.medworm.com/index.php?rid=609891&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22692 Ewing sarcoma/peripheral neuroectodermal tumor (EWS/PNET), since its characterization immunophenotypically and cytogenetically, has emerged as one of most common sarcomas of childhood. Currently, it is recognized that EWS/PNET can occur in any number of extraosseous sites and is one of several distinctive tumor types with an EWS translocation. In the past, the pathologic diagnosis of EWS/PNET relied on an open biopsy with the application of various ancillary studies, ranging from periodic acid-Schiff stain to molecular testing, but the tumor increasingly is diagnosed on the basis of cytologic specimens alone.The authors report 3 cases of EWS/PNET in patients aged 11 years to 53 years. These 3 patients had tumors that involved the parotid gland, cervical soft tissue, and pelvis, and were di... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=609891 Tue, 08 May 2007 04:00:00 +0100 609891 http://www.medworm.com/index.php?rid=609890&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22751 There is justifiable excitement about the recent introduction of prophylactic vaccines against human papillomavirus (HPV) types 16 (HPV-16) and HPV-18. Preventing these infections theoretically could avert approximately 70% of cervical cancer cases worldwide. In the U.S., numerous influential advocates are calling for universal vaccination of adolescent females. Given the promise of the vaccines, perhaps it is inevitable that vaccine introduction is proceeding before full consideration of how universal vaccination would affect existing, successful cervical cancer prevention programs. Determining the impact and cost effectiveness of the vaccines unavoidably will require time. Nevertheless, it is worth describing in broad terms for the readers of Cancer Cytopathology how successful, broad HP... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=609890 Tue, 08 May 2007 04:00:00 +0100 609890 http://www.medworm.com/index.php?rid=609889&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22752 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=609889 Tue, 08 May 2007 04:00:00 +0100 609889 http://www.medworm.com/index.php?rid=594493&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22689 Deep fibromatosis or desmoid tumor (DF/DT) is a low-grade, soft tissue lesion that is notable for its infiltration and local recurrence and its inability to metastasize. Although the histologic features of DF/DT are well described, there is a paucity of literature regarding cytologic findings.The surgical pathology files of The Johns Hopkins Hospital revealed 17 patients with a diagnosis of DF/DT with prior cytology in a 16-year period (1989-2005). The clinicoradiologic findings were noted, and the fine-needle aspiration (FNA) slides were available for review in 8 patients. In patients with archived tissue, an immunohistochemical panel was performed that included [beta]-catenin, desmin, CD-34, and c-kit.There was a wide age range and a wide range of anatomic distribution for DF/DT in this ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=594493 Mon, 07 May 2007 04:00:00 +0100 594493 http://www.medworm.com/index.php?rid=594494&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22688 The objective of this study was to assess the rate and possible reasons for false-negative (FN) reflex human papillomavirus (HPV)-DNA tests.The authors reviewed all ThinPrep cervical specimens that were submitted for reflex HPV-DNA testing using the Digene Hybrid Capture II (HC2) method from January 2002 to January 2004. Follow-up biopsies were reviewed. The results were considered HPV-FN if the HPV-DNA test was negative and the biopsy was positive for grade [ge]2 cervical intraepithelial neoplasia (CIN2+), and the results were considered true positive (HPV-TP) if the HPV-DNA test was positive and the biopsy showed CIN2+. HPV-FN cases were compared with HPV-TP cases regarding the grade and extent of CIN, the number of abnormal cells on the original ThinPrep slide, and the presence of ampli... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=594494 Thu, 03 May 2007 04:00:00 +0100 594494 http://www.medworm.com/index.php?rid=594495&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22690 Ductal lavage (DL) does not routinely identify cytologically malignant cells. For this study, the authors asked whether molecular analyses of DL specimens from women with cancer would identify abnormal cells, even if they appeared cytologically normal.DL was performed and yielded fluid in 29 of 45 consenting women who were undergoing breast cancer surgery. Array comparative genomic hybridization (CGH) was performed on the corresponding tumor tissue from 14 women. There was no single, common alteration; thus, bacterial artificial chromosome-specific fluorescence in situ hybridization (FISH) probes were selected based on CGH alterations.FISH copy number changes were detected in tumor sections in 9 women. In the corresponding 9 DL samples, 1 sample was clearly malignant on cytology, 1 showed ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=594495 Tue, 01 May 2007 04:00:00 +0100 594495 http://www.medworm.com/index.php?rid=454829&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22489 The effectiveness of fine-needle aspiration biopsy (FNAB) for rendering a specific diagnosis can be improved by applying several ancillary modalities. This review details several applications of molecular techniques using FNAB specimens with an emphasis on those used for patient care. A detailed search of the literature was conducted to collect all reports that used FNAB for different types of molecular tests. Several types of molecular tests, including in-situ hybridization, polymerase chain reaction, Southern blotting, and gene microarrays using FNAB specimens have been reported. These tests have been used with different organ systems and different objectives, including the detection of cancer cells, diagnosis, distinction of benign and malignant disease, prediction of response to chemot... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=454829 Wed, 07 Mar 2007 05:00:00 +0100 454829 http://www.medworm.com/index.php?rid=454833&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22576 With wide use of fine-needle aspirates (FNA) for investigation of thyroid nodules, a growing number of parathyroid glands are being inadvertently aspirated for cytologic study. Aiming to determine the effectiveness of FNA to differentiate between parathyroid and thyroid lesions, all cases aspirated initially as thyroid nodules and that had a final histologic diagnosis of parathyroid lesion were retrieved from the authors' files and were systematically reviewed to delineate possible specific diagnostic criteria.From a total of 90,000 FNA diagnoses of thyroid nodules, 4740 cases were isolated for which a final histologic diagnosis was available. Among these, 29 cases with a final histologic diagnosis of a parathyroid lesion were identified and reanalyzed according to the initial cytologic di... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=454833 Fri, 02 Mar 2007 05:00:00 +0100 454833 http://www.medworm.com/index.php?rid=454832&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22579 To assess the role of cytology in tumor diagnosis and to explore the potential of this technique to improve tumor registry quality, the authors investigated the role of cytology as a diagnostic tool in registry databases.Through the Italian Network of Cancer Registry (AIRTum) archive, the authors retrieved tumors diagnosed during the years 1983-2002 from several registries, Varese, Torino, Ragusa, Ferrara, Genova, and the Tuscan Cancer Registry. The authors then analyzed the amount of morphological confirmation by topographic code, distinguishing cytological from histological diagnosis. The authors analyzed, only for the Tuscan Cancer Registry, the amount of morphological confirmation by both histological and cytological diagnosis and demonstrated the variation of cytological confirmation ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=454832 Fri, 02 Mar 2007 05:00:00 +0100 454832 http://www.medworm.com/index.php?rid=454831&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22580 The aim of this study was to examine p16INK4a protein expression in ThinPrep (Cytyc Corporation, Marlborough, Mass) cervical specimens by using the CINtec p16INK4a Cytology Kit (Dako, Glostrup, Denmark). The ability of this assay to accurately identify underlying high-grade lesions was assessed by using follow-up biopsies and comparing these results with Hybrid Capture 2 (Digene, Gaithersburg, Md) high-risk HPV (hc2) results.Three hundred ninety-eight residual ThinPrep samples were collected, and histological follow-up data were retrieved for abnormal cytology specimens. After preparation of a Papanicolaou-stained slide, a second slide was processed in preparation for p16INK4a immunostaining. High-risk human papillomavirus testing (hc2) was also performed.Of the 163 cytologically abnormal ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=454831 Fri, 02 Mar 2007 05:00:00 +0100 454831 http://www.medworm.com/index.php?rid=454837&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22488 Inappropriate use of the category of atypical squamous cells of undetermined significance (ASCUS) can result in overtreatment or undertreatment of patients, which may decrease the cost effectiveness of screening. Quality assurance tools, such as the ASCUS to squamous intraepithelial lesion ratio (ASCUS:SIL) and case review, are imperfect. High-risk HPV (hrHPV) testing is an objective test for a known viral carcinogen, and hrHPV may be more useful in monitoring the quality of ASCUS interpretations.hrHPV rates for cytologic diagnoses and patient age groups were calculated for a 2-year period. All hrHPV results for ASCUS and SIL over a 17-month period were analyzed by patient age group, over time, and by individual cytopathologist to compare hrHPV rates with the corresponding ASCUS:SIL.The hr... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=454837 Wed, 28 Feb 2007 05:00:00 +0100 454837 http://www.medworm.com/index.php?rid=454836&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22575 This study compares diagnostic accuracy and morphology of TP and CS in SG FNABs.The authors retrospectively reviewed 98 satisfactory SG FNABs with both TP and CS. All cases had surgical resection. CS and TP slides were assessed for multiple morphological parameters, as well as the ability to make the diagnosis. Chi-square analysis was performed to compare CS and TP.An accurate diagnosis was rendered more commonly with CS compared with TP (57% versus 42%; P = .032), whereas the unsatisfactory rate was greater with TP compared with CS (19% versus 9%; P = .041). The error (4%) and indeterminate (35%) rates for TP were similar to CS. The diagnostic yield was greater for cellular cases, which were more frequent with CS compared with TP, than for cases of low cellularity; the diagnostic yield of... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=454836 Wed, 28 Feb 2007 05:00:00 +0100 454836 http://www.medworm.com/index.php?rid=454835&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22577 Colposcopy biopsy procedure is a standard recommendation for atypical squamous cell cannot exclude high-grade lesion (ASC-H) in abnormal Papanicolaou smears. p16 (p16INK4a), a cell cycle regulator, has been shown to be overexpressed in squamous dysplasia. To further improve the diagnostic accuracy of the ASC-H Papanicolaou smear and to reduce unnecessary procedures, the authors evaluated the utility of immunodetection of p16 in liquid-based cytology specimens on cell blocks.Seventy-five liquid-based (SurePath; TriPath Imaging, Inc. Burlington, NC) cytology specimens were prepared for cell blocks. Three groups (G1, G2, and G3) of cases were included: G1 comprised 44 cases of ASC-H; G2, 14 cases of high-grade dysplasia; and G3, 17 negative/reactive cases. All cases in G1 were confirmed by ce... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=454835 Wed, 28 Feb 2007 05:00:00 +0100 454835 http://www.medworm.com/index.php?rid=454834&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22578 AutoPap-assisted smear reading has been proposed prior to conventional manual reading; the latter may be unnecessary for cases reported as No Further Review (NFR) and would be required for cases reported as Review (REV).The authors evaluated comparable concurrent screening cohorts who were undergoing a conventional manual (CONV) or an AutoPap-assisted smear reading within the same screening program. The authors evaluated the prevalence of CIN2+ at repeat screening in subjects 1) with a negative report at conventional Papanicolaou test (CONV- = 9605), 2) with a REV report at AutoPap, followed by a negative conventional reading (REV- = 17,576), and 3) with a NFR report at AutoPap, followed by a negative rapid review (NFR- = 3477) at previous (baseline) screening.Crude CIN2+ detection rate wa... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=454834 Wed, 28 Feb 2007 05:00:00 +0100 454834 http://www.medworm.com/index.php?rid=388095&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22487 The objectives of this study were 1) to estimate the total annual Pap tests performed in the US based on recent NHIS surveys, and 2) to estimate the potential change in the total annual Pap volume produced by changing demographics, reduced screening frequency, HPV testing, and the HPV vaccine.In the NHIS 2000 and NHIS 2005, women were asked to report the frequency of their Pap tests for the 6 years prior to the interview and to report whether they had abnormal findings. The authors analyzed the survey respondents answers to these questions by using SAS Survey Procedures (SAS Institute, NC). The results were stratified by age, and the total national volume was then extrapolated from a similarly stratified 2000 US census. The projected increase of total Pap tests for the next 25 years was de... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=388095 Wed, 31 Jan 2007 07:00:00 +0100 388095 http://www.medworm.com/index.php?rid=388096&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22424 Following The Bethesda System 2001 (TBS 2001) recommendation to report normal endometrial cells (nEMC) in women ages [ge]40 years, studies have shown that endometrial (EM) sampling has increased, but detection of significant EM pathology has not increased. The cost implications of this increased EM sampling have not been specifically addressed. The aim of this study was to assess the cost effectiveness of EM sampling in women ages [ge]40 years with nEMC in their Papanicolaou tests.The authors reviewed 499 cases at a large academic women's hospital where nEMC had been reported in Papanicolaou tests followed by EM tissue sampling. Relevant clinical information was obtained from cytopathology and surgical pathology reports. Data on costs of EM sampling were obtained from business offices.Of 1... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=388096 Mon, 29 Jan 2007 07:00:00 +0100 388096 http://www.medworm.com/index.php?rid=350826&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22425 Cervical cancer is the second most common cancer in women worldwide. Human papillomavirus (HPV) is the etiologic agent for the vast majority of premalignant and malignant lesions, and high-risk HPV types can be detected in almost all cases of cervical dysplasia and carcinoma. HPV testing has been widely adopted for the triage of patients after a cervical cytology screening test (Papanicolaou smear or liquid-based cervical cytology such as ThinPrep or SurePath) interpretation of atypical squamous cells of undetermined significance (ASCUS), and HPV testing is increasingly used for screening in conjunction with cervical cytology. Although cervical cytology is a highly effective screening test for cancer, it has limited specificity for clinically significant lesions in cases with low-grade cyt... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=350826 Fri, 12 Jan 2007 05:00:00 +0100 350826 http://www.medworm.com/index.php?rid=328123&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22420 The identification of a small percentage of high-grade cervical intraepithelial neoplasias (HGCIN) among patients with minor cytological abnormalities (atypical squamous cells of undetermined significance [ASCUS] and/or low-grade squamous intraepithelial lesions [LSIL] group) is a major problem in cytology-based cervical cancer screening. The authors investigated the efficacy of p16INK4a as a biomarker to identify samples of patients with HGCIN among those with an ASCUS or LSIL result in Papanicolaou cytology.Consecutive liquid-based cytology specimens of 137 ASCUS and 88 LSIL results were selected from gynecologists who adopted a triage regimen with biopsy under colposcopy 2 months later, independent of the p16INK4a result. p16INK4a stained slides were prepared and independently read by 2... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=328123 Thu, 21 Dec 2006 07:00:00 +0100 328123 http://www.medworm.com/index.php?rid=328122&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22423 The Bethesda System (TBS) along with its companion atlas was updated in 2001 to improve standardization, clarity, and reproducibility of cervical cytology reporting.The authors used a novel web-based format to compare assessments of 77 images demonstrating a range of classical and borderline cytologic changes by a self-selected group of United States cytotechnologists (n = 216) and pathologists (n = 185).Participants were highly experienced, with 71.2% of cytotechnologists and 53.0% of pathologists reporting >10 years of practice. The mean percentage of exact agreement with the panel was slightly though significantly higher for cytotechnologists (57.0%) compared with pathologists (53.4%), adjusted for experience (P = .004); cervical cytology percentage effort (P = .0005); or cervical acces... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=328122 Thu, 21 Dec 2006 07:00:00 +0100 328122 http://www.medworm.com/index.php?rid=323530&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22419 Differentiating primary glandular from high-grade squamous intraepithelial lesions (HSIL) that involve endocervical glands is not an uncommon diagnostic problem in liquid-based gynecological cytology. Squamous and atypical glandular cell lesions may show similar cytomorphologic features. The aim of this study was to evaluate the use of p63 as a marker of basal and/or squamous cell derivation in this differential diagnosis.Of 59,257 liquid-based cervicovaginal specimens collected over a 3-year period, 149 were diagnosed as atypical glandular cells of uncertain significance (AGUS) or adenocarcinoma and had histological follow-up. Ten cases (8AGUS and 2 adenocarcinomas) were proven to be high-grade dysplasia on cervical biopsies and the remaining cases represented glandular pathology. Slides ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=323530 Mon, 18 Dec 2006 05:00:00 +0100 323530 http://www.medworm.com/index.php?rid=323532&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22421 This study was designed to compare prechemotherapy and postchemotherapy cytomorphology of DSRCT and to evaluate for distinct chemotherapy-induced changes.The authors searched their respective institutional databases for all DSRCT cases with an associated FNAB. FNAB slides, immunocytochemistry, and cytogenetic results were reviewed.Six aspirates from 5 patients were identified, 3 of which were postchemotherapy. The postchemotherapy cases demonstrated cytologic findings not typically described in DSRCTs, including prominent and conspicuous nucleoli, discohesive single-cell architecture, and slightly larger cell size.Cytomorphologic variability was prominent in prechemotherapy cases, and no case could be classified as DSRCT on cytology alone; immunohistochemistry was necessary for definitive ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=323532 Fri, 15 Dec 2006 07:00:00 +0100 323532 http://www.medworm.com/index.php?rid=323531&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22422 Most cystic pancreatic neoplasms are currently evaluated by an endoscopic ultrasound-guided fine-needle aspiration biopsy (FNAB). In the authors' experience, FNAB of cystic pancreatic endocrine tumors (PETs) frequently causes diagnostic difficulties, partly because of unexpected overlapping features with cystic ductal adenocarcinomas.The authors identified 5 histologically confirmed cystic PETs that were evaluated by FNAB and compared their cytomorphologic features to cystic ductal adenocarcinomas (n = 5) and solid PETs (n = 39) of the pancreas.Cytologically, 2 of the aspirates of cystic PETs were devoid of tumor cells whereas the other 3 were variably cellular and composed of cohesive aggregates of monomorphic cellular elements with variably coarse chromatin. Tumor necrosis and nuclear me... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=323531 Fri, 15 Dec 2006 07:00:00 +0100 323531 http://www.medworm.com/index.php?rid=270240&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22283 Introduction of nonmicroscopic cervical screening techniques creates the potential for liquid-based cytology specimens to be sent for human papillomavirus (HPV) testing, automated screening, or other assays prior to microscopic quality assessment. It was hypothesized that the volumes required to prepare ThinPreps ("sip volumes") represent indicators of specimen quality.A stratified random sample of 505 enrollment ThinPreps were assessed in the Atypical Squamous Cells of Undetermined Significance/Low-Grade Squamous Intraepithelial Lesion Triage Study (ALTS) to evaluate associations between sip volume and slide cellularity, cellular distribution, and clinical outcomes. Masked assessments included counting cells and qualitative evaluations.Sip volumes were highest among women aged 18-19 or [g... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=270240 Fri, 10 Nov 2006 23:50:03 +0100 270240 http://www.medworm.com/index.php?rid=270239&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22287 Patients with noninvasive, small-sized primary adenocarcinomas of the lung have excellent prognosis after lobectomy. Several researchers have suggested that limited resection could be an acceptable alternative for these patients. Therefore, a preoperative or intraoperative judgment of invasiveness would be one of the critical determinants of the surgical procedure in each case. Cytopathologic findings that can distinguish invasive from noninvasive adenocarcinomas remain to be elucidated.Imprint smears were obtained from 60 resected adenocarcinomas with nonmucinous bronchioloalveolar features. Thirteen cytologic factors were evaluated: the presence of necrosis, fibrovascular tissue, proportion of macrophages, the presence of large tumor cell clusters, nuclear grooves, nuclear overlapping, v... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=270239 Fri, 10 Nov 2006 07:00:00 +0100 270239 http://www.medworm.com/index.php?rid=267752&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22285 Testing for human papillomavirus (HPV) is an integral part of equivocal cervical cytology triage. Clinical validation of non-FDA (Food and Drug Administration)-approved methods is therefore important because of the high volume of such tests and the implications for missed high-grade lesions if test performance is not optimal.A preinitiation study and 17 months of follow-up data using Hybrid Capture II (HC II) HPV detection with SurePath (SP) sample collection were analyzed. Results of HPV tests on abnormal cytology samples were collected and compared with follow-up results. HPV-positive rates were determined in cases of low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion (HSIL), and follow-up rates of cervical intraepithelial neoplasia (CIN) were... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=267752 Thu, 09 Nov 2006 06:28:04 +0100 267752 http://www.medworm.com/index.php?rid=267751&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22286 Equivocal atypia in respiratory cytology can be a diagnostic challenge. In such cases fluorescence in situ hybridization (FISH) may be used for the analysis of chromosomal aberrations and often allows a reliable distinction of benign and malignant cells.An online picture gallery of 30 respiratory cytologic preparations comprising 23 specimens with equivocal cytology as well as 5 positive and 2 negative controls was prepared (). The final diagnoses were confirmed by clinical follow-up or biopsy or both. Each of the illustrated cell groups was analyzed by multitarget FISH after PAP image capturing and automatic relocalization.The online questionnaire was completed by 137 cytomorphologists from all continents. The control cases were assessed accurately to a significantly higher percentage tha... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=267751 Thu, 09 Nov 2006 05:00:00 +0100 267751 http://www.medworm.com/index.php?rid=258504&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22284 Evidence suggests that overexpression of p16INK4a protein indicates infection and genomic integration of high-risk human papillomavirus (HR HPV) and predicts progression to cervical high-grade squamous intraepithelial lesions (HSILs) and carcinoma. The authors compared the ability of p16INK4a and HR HPV detection by Hybrid Capture 2 (HC2) to detect the presence of significant cervical disease.Four hundred ThinPrep® specimens (100 each in 4 categories: 100 specimens that were negative for intraepithelial lesions, 100 specimens of atypical squamous cells of undetermined significance [ASC-US], 100 specimens of low-grade squamous intraepithelial lesions [LSILs], and 100 specimens of HSILs) were analyzed. p16INK4a protein was immunolocalized using a specific monoclonal antibody, and the detect... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=258504 Thu, 02 Nov 2006 07:00:00 +0100 258504 http://www.medworm.com/index.php?rid=249541&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22288 Routine liquid-based cytology (LBC) provides excellent sensitivity for the detection of cervical high-grade squamous intraepithelial lesion (HSIL); however, its specificity is low. Consequently, many women who have atypical squamous cells of undetermined significance (ASC-US) or low-grade squamous intraepithelial lesion (LSIL) cytology undergo unnecessary colposcopy. The authors hypothesized that a novel immunocytochemical assay (ProExTM C) that can be performed on LBC slides had a significantly higher positive predictive value (PPV) for biopsy-proven HSIL compared with routine LBC.The ProExTM C immunocytochemical assay utilizes a cocktail of monoclonal antibodies directed against proteins associated with aberrant S-phase cell cycle induction (topoisomerase IIA, minichromosome maintenance ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=249541 Thu, 26 Oct 2006 11:38:01 +0100 249541 http://www.medworm.com/index.php?rid=249540&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22289 Lymphoepithelial cysts (LECs) of the pancreas are extremely rare, benign, nonneoplastic cysts that can mimic pseudocysts or cystic neoplasms clinically and radiographically. The cytologic features of LECs have been described only in a handful of case reports and may overlap with both benign and malignant pancreatic tumors.The authors conducted a 5-year, retrospective, computerized review of the cytopathology files of 2 institutions for all diagnoses of pancreatic LECs. Clinical, radiographic, cytologic, and chemical findings were reviewed.Four patients were identified. The study group consisted of 3 men and 1 woman who ranged in age from 33 years to 63 years. The masses were located throughout the pancreas. Tumor sizes ranged from 1.8 cm to 5.7 cm in greatest dimension. Smears from all pat... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=249540 Thu, 26 Oct 2006 11:38:01 +0100 249540 http://www.medworm.com/index.php?rid=249539&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22305 In this study, the course of HR-HPV infection in 703 women was observed. From a database of 720,016 negative cytology smears, the authors selected 703 women based on the availability of at least 2 HR-HPV polymerase chain reaction tests. The authors database stores not only the HPV data but also all other cytologic and histologic data, allowing the detection of women who progressed from negative cytology to CIN within a period of 10 years.Of the 703 selected women, 159 were found to have alternating HR-HPV infection (change from a negative HR-HPV test to a positive test or vice versa), 40 had a persistently positive HR-HPV test, and 504 women had a persistently negative HR-HPV test. The percentage of alternating HPV infection declined over time from 37% to 7%. Of the women age older than 40... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=249539 Thu, 26 Oct 2006 06:00:00 +0100 249539 http://www.medworm.com/index.php?rid=201767&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22170 The objective of this study was to investigate the clinical implications and pathologic significance of ASC-H in pregnant women, so that these patients will be managed appropriately.All Papanicolaou tests that were diagnosed as ASC-H in pregnant women over 1.5 years (total, 60 women) were reviewed and correlated with histologic and/or cytologic follow-up. High-risk type of human papillomavirus (HPV) status was also correlated with follow-up findings. The following cytomorphologic parameters were evaluated for each woman and were compared between the squamous intraepithelial lesion (SIL) follow-up group and the benign follow-up group: inflammatory background, the number of atypical cells, cell arrangement pattern, nuclear irregularity/grooves, hyperchromasia, and cell shape.Among 30 women w... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=201767 Sat, 23 Sep 2006 04:00:00 +0100 201767 http://www.medworm.com/index.php?rid=199657&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.21844 The 2001 Bethesda System category of atypical squamous cells (ASC) denotes changes suggestive, but inconclusive for, a squamous intraepithelial lesion (SIL). ASC is subcategorized as: 1) "undetermined significance (ASC-US)," when changes suggest low-grade or indeterminate-grade SIL and 2) "cannot exclude high-grade squamous intraepithelial lesion (ASC-H)," when a cancer precursor is suspected.To better define the characteristics of ASC-H, the authors analyzed and compared human papillomavirus (HPV) testing data and outcomes after 2 years for participants in the Atypical Squamous Cells of Undetermined Significance Low-Grade SIL Triage Study (ALTS), a randomized trial of 5060 women.Among women with thin-layer cytology findings of ASC-H, 84% tested positive for HPV, 50% (95% confidence interv... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199657 Thu, 21 Sep 2006 11:27:01 +0100 199657 http://www.medworm.com/index.php?rid=199656&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22169 Early cytologic detection and treatment of high-grade squamous intraepithelial lesion (HSIL) is critical to cervical cancer prevention. The term atypical squamous cells (ASC), cannot exclude HSIL (ASC-H) was introduced in 2001 in the Bethesda System (TBS 2001) to define changes suggestive, but not diagnostic, of HSIL in the absence of unequivocal squamous intraepithelial lesion (SIL). Previous studies showed that women with ASC-H cytology are at an increased risk of harboring underlying histopathologic HSIL. TBS 2001, however, did not address the significance of finding ASC-H changes in a background of unequivocal low-grade SIL (LSIL). There may be a tendency for cytologists to lump these changes with either LSIL or HSIL, depending on their level of comfort. In their laboratory, the author... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199656 Thu, 21 Sep 2006 11:27:01 +0100 199656 http://www.medworm.com/index.php?rid=199655&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22171 Cervical cytopathology has limited specificity for the detection of underlying clinically significant lesions in cases with low-grade cytologic abnormalities. The current study evaluated the performance of a novel immunocytochemical test (ProEx C) for topoisomerase II alpha (TOP2A) and minichromosome maintenance protein 2 (MCM2) in normal versus high-grade squamous intraepithelial lesion (HSIL) and positive control (SiHa) pooled cytology preparations and in a pilot series of prospectively collected patient specimens.TOP2a and MCM2 were detected as markers of aberrant S-phase induction in SurePath cervical cytology specimens by an indirect polymer-based immunoperoxidase method (ProEx C, TriPath Oncology, Burlington, NC). Slides were scored based on specimen adequacy, the presence of nuclear... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199655 Thu, 21 Sep 2006 11:27:01 +0100 199655 http://www.medworm.com/index.php?rid=199654&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22173 In this study, the feasibility of grading FL using ThinPrep (TP) slides and flow cytometry (FC) was evaluated.Fifty-three cases of lymph node fine-needle aspiration (FNA) from patients with histologically confirmed FL (20 Grade 1, 17 Grade 2, and 16 Grade 3) were included. The number of centroblasts present in 300 lymphoid cells and in 10 HPF in TP Papanicolaou-stained slides was evaluated. The percentage of CD10-positive small cells was calculated with FC results. Statistical analysis was performed with the Jonckheer-Terpstra nonparametric trend test and the Wilcoxon rank sum test.The statistical analysis demonstrated a significant upward trend in the number of centroblasts as the grades increased. Also, all 3 methods had statistically significant results to distinguish different grades o... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199654 Thu, 21 Sep 2006 11:27:01 +0100 199654 http://www.medworm.com/index.php?rid=199653&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22166 Efficient quality control is essential to ensure high sensitivity of Papanicolaou (Pap) smears. For this purpose, rescreening of 10% random negative smears is increasingly felt to be ineffective. Rapid rescreening (RR) of all negative Pap smears is more practical and has received widespread acceptance, especially in Europe, although its sensitivity is difficult to monitor and its retrospective nature may influence the vigilance of the screeners. The method of rapid prescreening (RPS) overcomes these drawbacks because rapid review of Pap smears precedes full screening.All routine conventional Pap smears (n = 8364) over 2 months underwent RPS by 12 cytotechnologists, followed by full screening. Data were analyzed to determine correlation between the RPS sensitivity of individual cytotechnolo... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199653 Thu, 21 Sep 2006 11:27:01 +0100 199653 http://www.medworm.com/index.php?rid=199652&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22167 The degree of applicability of the Bethesda System 2001 (TBS 2001) for cervicovaginal cytology to a public health setting is unknown, and extrapolations from available data are unwarranted.A "before/after" study design was used to evaluate the impact of TBS 2001 on an organized, population-based screening program in northern Italy. Between 2003-2004, 6 cytology laboratories converted from TBS 1991 to TBS 2001. A set of screening indicators based on TBS 2001 (85,012 patients) were compared with those based on TBS 1991 (199,833 patients) by means of their laboratory- and patient age-standardized ratio with a 95% confidence interval (CI).The prevalence of cervical intraepithelial neoplasm (CIN)2-3/carcinoma was stable between the 2 populations. TBS 2001 had no effect on the unsatisfactory rat... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199652 Thu, 21 Sep 2006 11:27:01 +0100 199652 http://www.medworm.com/index.php?rid=199651&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22168 In this study, the objective was to determine whether these 2 morphologic subcategories are characterized by differences in risk for CIN3 and/or HPV type distribution.Within the Atypical Squamous Cells of Undetermined Significance/Low-Grade Squamous Intraepithelial Lesion Triage Study, all cytologic interpretations of HPV cellular changes and CIN1 rendered by any of the pathology reviewers (community laboratory, clinical center, or Pathology Quality-Control Group) on referral Papanicolaou (Pap) tests or enrollment ThinPrep® Pap tests were included for analysis. HPV testing was performed by Hybrid CaptureTM 2 (HC2) and by polymerase chain reaction based reverse-line blot analysis for 27 HPV types. The absolute risks of cumulative detection of CIN3 or cancer (CIN3 +) and CIN2 or worse (CIN2... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199651 Thu, 21 Sep 2006 11:27:01 +0100 199651 http://www.medworm.com/index.php?rid=199650&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22172 CD57 (Leu7), a marker for natural killer lymphocytes, and glucose transporter-1 (GLUT-1), a facilitative cell surface glucose transport protein, are expressed in a wide spectrum of epithelial malignancies. The usefulness of CD57 and GLUT-1 immunostaining was evaluated as an adjunct to assist in the diagnosis of papillary carcinoma in fine-needle aspirations (FNAs) of the thyroid.Immunohistochemical staining for CD57 and GLUT-1 was performed on paraffin-embedded cell blocks of 50 thyroid FNA cases with the following cytologic diagnoses: 1) papillary carcinoma (15 cases); 2) atypical cytology, cannot exclude papillary carcinoma (14 cases); and 3) benign thyroid (21 cases).Fourteen of 15 cases with an unequivocal diagnosis of papillary carcinoma were positive for CD57 and 1 case was negative ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199650 Thu, 21 Sep 2006 11:27:01 +0100 199650 http://www.medworm.com/index.php?rid=199649&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22174 The majority of patients with lymphoma undergo a single biopsy for diagnosis, and there are few opportunities to acquire posttreatment material. Fine-needle aspiration (FNA) biopsy is a minimally invasive procedure, and acquiring routine posttreatment material would require minimal effort and provide needed material for gene expression profiling.Ex vivo FNA biopsies were performed using a standard clinical technique with 21-gauge, 22-gauge, 23-gauge, and 25-gauge needles for 8 lymph node specimens and were collected in either RNA stabilization reagent (RNAlater) or Trizol. Eight patients with known or suspected Non-Hodgkin lymphoma (NHL) underwent interoperative (in vivo) FNA biopsies based on the best technique derived from the ex vivo aspirates. RNA derived from the in vivo FNA biopsies ... Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199649 Thu, 21 Sep 2006 11:27:01 +0100 199649 http://www.medworm.com/index.php?rid=199648&cid=s_33596_32_f&fid=33596&url=http%3A%2F%2Fdx.doi.org%2F10.1002%252Fcncr.22175 No abstract. (Source: Cancer Cytopathology) Cancer Cytopathology journals http://www.medworm.com/rss/comments.php?id=199648 Thu, 21 Sep 2006 04:00:00 +0100 199648