MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Cell Biology International' source. Tue, 07 Feb 2012 08:48:52 +0100 http://www.medworm.com/index.php?rid=5659183&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22295975%26dopt%3DAbstract Authors: Kwiatkowska M, Popłońska K, Wojtczak A, Stępiński D, Polit JT Abstract Lipid bodies present in lipotubuloids of O. umbellatum ovary epidermis take the form of a lens between leaflets of endoplasmic reticulum (ER) membrane filled with a highly osmiophilic substance. The two enzymes, diacylglycerol acyltransferase 1 and 2 (DGAT1 and DGAT2), involved in this process are synthesized on rough ER and localized in the ER near a monolayer surrounding entities like lipid bodies. After reaching appropriate sizes, newly-formed lipid bodies transform into mature spherical lipid bodies filled with less osmiophilic content. They appear to be surrounded by a half-unit membrane, with numerous microtubules running adjacentli in different directions. ER no longer continuous with lipid b... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5659183 Thu, 02 Feb 2012 05:00:00 +0100 5659183 http://www.medworm.com/index.php?rid=5659181&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22299633%26dopt%3DAbstract Authors: Xiao Z, Yang M, Li F, Lv Q, He Q, Deng M, Liu X, Chen X, Chen M, Xie X, Hu J Abstract Extracellular nucleotides mediate a wide range of physiological effects by interacting with plasma membrane P2 purinergic receptors. Recently, it had been reported that P2 receptors are expressed in certain kinds of stem cells, and function to induce cytokine expression and to modulate cell proliferation. In the present study, we analyzed the expression and the function of P2 receptors in human umbilical cord blood-derived EPCs. We demonstrated here that EPCs expressed P2X4,6,7 and P2Y2,4,11,13,14 receptors and extracellular ATP inhibited EPCs proliferation. In accord with our previous study, we found that EPCs expressed functional TLR4 and the activation of TLR4 by LPS evoked a proinflam... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5659181 Thu, 02 Feb 2012 05:00:00 +0100 5659181 http://www.medworm.com/index.php?rid=5659182&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22296025%26dopt%3DAbstract This report deals with other mechanisms to explain the known heterogeneous distribution of ions. To study other mechanisms it was necessary to turn to a non-living system, specifically tendon/collagen to eliminate the contribution of the membrane pump and channels. Thus a simple gravimetric method was designed to measure solute accumulation or exclusion during rehydration of a well washed, carefully dried and well characterized protein specimen (tendon/collagen). Exposure to high salt concentrations resulted in selective exclusion of Na+ over K+ while exposure to low salt concentration resulted in accumulation of these solutes. It is postulated that this solute redistribution occurs in all hydrated proteins and is partially responsible for the heterogeneous solute distribution in cells pre... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5659182 Wed, 01 Feb 2012 05:00:00 +0100 5659182 http://www.medworm.com/index.php?rid=5659184&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22288713%26dopt%3DAbstract Authors: Sun R, Zhu Z, Su Q, Li T, Song Q Abstract Bacterial endotoxins may lead to vascular endothelial cell injury. Our study aims to explore the role of Toll-like receptor 4 (TLR4) and stromal interaction molecule 1 (STIM1) in bacterial endotoxin-induced calcium overload and inflammatory reactions in human umbilical vein endothelial cells (HUVECs). Our study showed under lipopolysaccharide (LPS) stimulation, LPS binding protein (LBP) mRNA levels peaked at 24 h, TLR4 levels at 12 h, and nuclear factor κB (NFκB) levels at 6 h (all P < 0.01). LBP protein levels increased gradually and peaked at 24 h of LPS treatment. TLR4 protein levels increased significantly at 1 h and peaked at 12 h. NFκB protein levels markedly increased at 1 h and peaked at 6 h. Knockdown of STIM1 alone,... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5659184 Tue, 31 Jan 2012 05:00:00 +0100 5659184 http://www.medworm.com/index.php?rid=5659186&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22288569%26dopt%3DAbstract In conclusion, the transient exposure to NaBu after GVBD improved meiotic competence but not subsequent developmental competence in pig oocytes. The results may help us to understand the effect of histone acytelation during ooctye maturation and maybe helpful for in vitro embryo production. PMID: 22288569 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5659186 Mon, 30 Jan 2012 05:00:00 +0100 5659186 http://www.medworm.com/index.php?rid=5659185&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22288650%26dopt%3DAbstract Authors: Zhang J, Fang L, Zhu X, Qiao Y, Yu M, Wang L, Chen Y, Yin W, Hua ZC Abstract Ro52/SSA is an autoantigen which presents in patients with Sjogren's syndrome (SS) and systemic lupus erythematosus (SLE). Ro52/SSA was reported to increase cell death and redistributed itself to apoptotic blebs, but its proapoptotic function has not been completely identified. In the present study, we found that overexpression of Ro52/SSA promoted cell apoptosis induced by death receptor in caspase-8-dependent manner. Ro52/SSA expression down-regulated the expression of c-FLIP(L), and Ro52/SSA siRNAs increased c-FLIP(L) production, indicating that Ro52/SSA plays a role in c-FLIP(L) regulation. Furthermore, using reporter assays, we found Ro52/SSA negatively regulated c-FLIP(L) transcriptional lev... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5659185 Mon, 30 Jan 2012 05:00:00 +0100 5659185 http://www.medworm.com/index.php?rid=5640371&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22276695%26dopt%3DAbstract The objective of this study was to investigate the effect of 1α,25-Dihydroxyvitamin D3 (VD3) on the proliferating, differentiating and differentiated phases of C2C12 myoblasts, a mouse skeletal muscle cell line. In the proliferating phase, cell counting and WST-1 cell proliferation assay revealed that VD3 treatment under 10% FBS inhibited the proliferation and viability of the cells in a dose-dependent manner. Flow cytometric analysis showed that VD3 dose-dependently increased the percentage of cells in the G0/G1 phase. VD3 treatment in the differentiating phase inhibited the formation of myotubes and the expression of total MHC at both the mRNA and protein levels. VD3 treatment in the differentiated phase did not show significant difference in the amount of total MHC using western blot a... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5640371 Thu, 26 Jan 2012 05:00:00 +0100 5640371 http://www.medworm.com/index.php?rid=5640370&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22276743%26dopt%3DAbstract Authors: Meng W, Jiang L, Lu L, Hu H, Yu H, Ding D, Xiao K, Zheng W, Guo H, Ma W Abstract Substantial data indicate that the oncogene microRNA-155 is significantly elevated in glioblastoma multiforme (GBM) and regulates multiple genes associated with cancer cell proliferation, apoptosis, and invasiveness. Thus, miR-155 can theoretically become a target to enhance the chemotherapeutic effect in cancer therapy. So far, the effect of down-regulating miR-155 to enhance the chemotherapeutic effect to taxol has not been studied in human GBM. Human GBM U251 cell were treated with taxol and the miR-155 inhibitor, alone or in combination. The 50% inhibitory concentration and cell viability were determined by the MTT assay. Annexin V/PI staining was performed, and apoptosis and the cell cycl... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5640370 Thu, 26 Jan 2012 05:00:00 +0100 5640370 http://www.medworm.com/index.php?rid=5640369&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22280466%26dopt%3DAbstract Authors: Ostuni A, Infantino V, Salvia A, Miglionico R, Boraldi F, Annovi G, Bisaccia F Abstract Progressive calcification and fragmentation of elastic fibers are characteristic hallmarks of Pseudoxanthoma elasticum (PXE) which is caused by mutations in the ABCC6 gene encoding the multidrug resistance-associated protein 6 (MRP6). Several evidences suggested that PXE is a metabolic disorder but the exact mechanism by which ABCC6 mutations promote the elastic fiber mineralization is unknown. To shed light in this issue, we analyzed, by real-time PCR, the expression profile of a number of genes involved in matrix gla-protein (MGP) γ-carboxylation and in phosphate generation, in healthy and PXE dermal fibroblasts. Tissue nonspecific alkaline phosphatase (TNAP), NADPH: P450 reductase (... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5640369 Thu, 26 Jan 2012 05:00:00 +0100 5640369 http://www.medworm.com/index.php?rid=5640372&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22273270%26dopt%3DAbstract Authors: Kamada M, Kumazaki T, Matsuo T, Mitsui Y, Takahashi T Abstract In order to establish useful human normal cell lines, telomerase reverse transcriptase (TERT) cDNA was transfected into normal female lung fibroblast, TIG-1. After long term-subcultivation of 74 individual clones selected for resistance to G418, we obtained 55 cultures with normal range of life span [- 75 population doubling level (PDL)], 16 cultures with extended life span (75-140 PDL). In addition to them, three immortal cell strains and unexpectedly, one ultra long-lived cell line (ULT-1) with life span of 166 PDL were established. IMT-1, one of immortal cell strains was confirmed to maintain long telomere length, high telomerase activity and extremely low level of p16INK4A. They also showed mild level of p... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5640372 Wed, 25 Jan 2012 05:00:00 +0100 5640372 http://www.medworm.com/index.php?rid=5640373&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22272563%26dopt%3DAbstract The objective of the current study was to determine whether the syndecan-4 side chains regulate cell proliferation through muscle cell focal adhesion formation and apoptosis. Syndecan-4 mutants with only one or without any N-glycosylated chains attached to the core protein with or without GAG chains were generated to study the function of N-glycosylated chains and the interaction between N-glycosylated chains and GAG chains. The wild type syndecan-4 and all of the syndecan-4 side chain mutants were transfected into turkey myogenic satellite cells. Cell apoptosis and focal adhesion formation were measured, and protein kinase C alpha (PKCα) cell membrane localization was investigated. Syndecan-4 increased focal adhesion kinase (FAK) activity and the deletion of the side chains decreased thi... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5640373 Tue, 24 Jan 2012 05:00:00 +0100 5640373 http://www.medworm.com/index.php?rid=5623482&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22251027%26dopt%3DAbstract Authors: van Opstal A, Bijvelt J, van Donselaar E, Humbel BM, Boonstra J Abstract Inhibition of PKB activity using a highly selective PKB inhibitor resulted in inhibition of cell cycle progression only if cells were in early G1 phase at the time of addition of the inhibitor, as demonstrated by time-lapse cinematography. Addition of the inhibitor during mitosis up to 2 hours after mitosis, resulted in arrest of the cells in early G1 phase, as deduced from the expression of cyclins D and A and incorporation of thymidine. After 24 hours of cell cycle arrest, cells expressed the cleaved caspase-3, a central mediator of apoptosis. These results demonstrate that PKB activity in early G1 phase is required to prevent the induction of apoptosis. Using antibodies, it was demonstrated that ac... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5623482 Wed, 18 Jan 2012 05:00:00 +0100 5623482 http://www.medworm.com/index.php?rid=5602666&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22248258%26dopt%3DAbstract Conclusions: Blimp1 expression may be independent of the effect of atRA on PGC differentiation. atRA may promote the start of a period in which there is a low level of Lin28 expression during PGC differentiation. PMID: 22248258 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5602666 Tue, 17 Jan 2012 05:00:00 +0100 5602666 http://www.medworm.com/index.php?rid=5602667&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22233500%26dopt%3DAbstract In this study, we used the two-step enzymatic digestion and differential adhesion methods to isolate and purify sheep skeletal muscle satellite cells, identified the cells, and induced the differentiation of these cells to analyze their pluripotency. The results of these experiments showed that the most efficient method for the isolation of sheep skeletal muscle satellite cells was the type I collagenase and trypsin two-step digestion method and that the best conditions for the in vitro culture of sheep muscle satellite cells involved culturing the cells in medium containing 20% FBS + 10% horse serum. Immunofluorescence staining showed that sheep skeletal muscle satellite cells expressed Desmin, a-Sarcomeric Actinin, MyoD1, Myf5, and PAX7. After myogenic induction, multi-nucleated myotubes... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5602667 Wed, 11 Jan 2012 05:00:00 +0100 5602667 http://www.medworm.com/index.php?rid=5602668&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22233450%26dopt%3DAbstract Authors: Ma LL, Meng FB, Shi P, Li G, Pang X Abstract Umbilical cord blood (UCB) as a novel resource of mesenchymal stem cells (MSCs) had been widely accepted. The quantity and biological characteristics of UCB-MSCs from different gestational ages have not been well studied. In the present study, we quantified the number of MSC in UCB of different gestational ages using multi-color flowcytometer and compared the cell proliferation rates of these UCB-MSCs. MSCs are defined as CD44+/CD105+/CD34-/CD45-cell population and we found that the numbers of UCB-MSCs declined with the gestational ages. The cell proliferation rates of UCB-MSCs were significantly higher in the non-full-term UCB samples than those of full-term UCB samples. Therefore, we concluded that the non-full-term UCB sample... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5602668 Tue, 10 Jan 2012 05:00:00 +0100 5602668 http://www.medworm.com/index.php?rid=5560894&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22214228%26dopt%3DAbstract This study evaluate the effect of PectaSol on modification of Doxorubicin(Dox) cytotoxicity and apoptosis and cell cycle in prostate cancer cell lines (DU-145 & LNCaP). Combination of PectaSol and Dox resulted in a viability of 29.36% and 32.55% (p value<0.001) in the DU-145 and LNCaP cells. The IC50 values decreased 1.5 fold and 1.3 fold in the DU-145 and LNCaP cells, respectively.The exposure of the DU-145 cells to the combination of PectaSol and Dox, resulted in a reduction in p27 gene and protein expression (p value<0.001) . In the LNCaP cells, this combination, increased p53, p27 and Bcl-2 genes expression. Treatment with both drugs led to increase in Sub-G1 arrest (54.6% versus 12.2% in Dox) in DU-145. In LNCaP cells combination of both drugs led to an increased in G2-M arr... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5560894 Wed, 04 Jan 2012 05:00:00 +0100 5560894 http://www.medworm.com/index.php?rid=5560895&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22214203%26dopt%3DAbstract Authors: Mahinpour R, Riazi G, Shokrgozar MA, Sarbolouki MN, Ahmadian S, Hadi Alijanvand H, Azadmanesh K, Douraghi M, Heidari M, Naghdi Gheshlaghi Z, Moosavi-Movahedi AA Abstract Recent studies suggest that arsenical compounds exhibit a differential toxicity to cancer cells. Microtubules are dynamic polymers of tubulin dimers involved in cell proliferation, morphogenesis and organelle transport, which are a primary target of a number of anticancer drugs, such as arsenical compounds. During the present study, the interaction of 1-naphthylarsonic acid (1-NAA) was evaluated on microtubule polymerization under in vitro and cellular conditions. Microtubules were extracted from sheep brain. Transmission electron microscopy was used to show microtubule structure in the presence of 1-NAA. ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5560895 Tue, 03 Jan 2012 05:00:00 +0100 5560895 http://www.medworm.com/index.php?rid=5493936&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22142363%26dopt%3DAbstract Authors: Bryantseva SA, Zhapparova ON Abstract Bidirectional transport along microtubules is ensured by opposing motor proteins: cytoplasmic dynein that drives cargo to the minus-ends and various kinesins that generally move to the plus-ends of microtubules. Regulation of motor proteins that are simultaneously bound to the same organelle is required to maintain directional transport and prevent pausing of cargo pulled away by motors of opposite polarity. Debates of the recent decade have been focused on two possible mechanisms of such regulation: (i) coordination, which implies that only one type of motors is active at a given time, and (ii) tug-of-war, which assumes that both motors are active at the same time and that direction of transport depends on the outcome of motor's confr... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5493936 Mon, 12 Dec 2011 10:18:19 +0100 5493936 http://www.medworm.com/index.php?rid=5493935&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22145955%26dopt%3DAbstract In conclusion, the expression of LeY and FUT4 correlates with endometrial receptive capability. It may be the new markers for the evaluation of endometrial receptivity. PMID: 22145955 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5493935 Tue, 06 Dec 2011 05:00:00 +0100 5493935 http://www.medworm.com/index.php?rid=5421954&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22070412%26dopt%3DAbstract Authors: Wang P, Cheng Y Abstract The lymphatic system was first described at around the same time as the blood circulation centuries ago, but the biological function elucidation of LECs (lymphatic endothelial cells) is far less than that of BVECs (blood vascular endothelial cells). Since the discovery of molecular markers for LECs and exploration of lymphatic role in tumour metastasis, more attention has been given to basic lymphatic research. Approx. 150 known genes were found to be expressed at the mRNA and protein levels by LECs. These molecules play an important role in lymphangiogenesis, signalling, tumour metastasis, immune function and fluid transport. This review provides a brief outline of gene expression profile of LECs and the molecular biological function, which will g... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5421954 Sat, 19 Nov 2011 02:54:55 +0100 5421954 http://www.medworm.com/index.php?rid=5421953&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22070413%26dopt%3DAbstract Authors: Goryukhina OA, Martyushin SV, Pinaev GP Abstract The prospect of developing transport systems using histones for site-specific delivery of therapeutic agents that have poor penetration characteristics through cellular membranes and tissue barriers has been investigated. Histones immobilized on microspheres can also be used to modify surfaces intended for cell cultivation, facilitating adhesion, proliferation and network formation by interactions of cells through contacts with several microspheres. They can be applied to three-dimensional pore matrices that are designed for producing tissue-like structures in vitro. PMID: 22070413 [PubMed - in process] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5421953 Sat, 19 Nov 2011 02:54:46 +0100 5421953 http://www.medworm.com/index.php?rid=5421949&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22087578%26dopt%3DAbstract This study examined the effects of IL-6 and sIL-6r on the expression of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), the plasminogen activation system including tissue-type plasminogen activator (tPA), urokinase-type PA (uPA) and PA inhibitor type 1 (PAI-1) using chondrocytes derived from normal human femur cartilage. The cells were cultured with or without 50 ng/mL IL-6 and/or 30 ng/mL sIL-6r in the presence or absence of the janus kinase (JAK) 3 inhibitor WHI-P131 or the mitogen-activated protein kinase (MAPK)/extracellular signal protein kinase (ERK) kinase (MEK) inhibitor PD98059 for up to 28 days. The expression levels of MMPs, TIMPs, uPA, tPA and PAI-1 were examined at the mRNA and protein levels using a real-time polymerase chain reaction and en... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5421949 Thu, 17 Nov 2011 05:00:00 +0100 5421949 http://www.medworm.com/index.php?rid=5421950&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22082048%26dopt%3DAbstract Authors: Lin C, Wang H Abstract It was reported that the expression and activity of NADPH oxidase increased when HL-60 cells were induced into terminally differentiated cells. However, the function of NADPH oxidase in cell differentiation is not well elucidated up to now. In the present study, 150~500 μM H2O2 induced the differentiation of HL-60 cells, measured by phagocytosis of latex beads and cell electrophoresis. While two inhibitors of NADPH oxidase, diphenylene iodonium <st2:citation w:st="on">(DPI)</st2:citation> and apocynin <st2:citation w:st="on">(APO)</st2:citation>, blocked the differentiation potential of cells induced by 200 μM H2O2. Meanwhile, H2O2 stimulated the generation of intracellular superoxide (O2·-), which was decreased by these tw... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5421950 Tue, 15 Nov 2011 05:00:00 +0100 5421950 http://www.medworm.com/index.php?rid=5421952&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22073964%26dopt%3DAbstract Authors: Huang Y, Liu F, Zhao L, Wu H, Liu H Abstract In order to create novel potent and selective anticancer agents, the anticancer activities of 4-(4-fluorophenyl)amino-5,6,7-trimethoxyquinazoline on ten kinds of tumor cells were evaluated by MTT assay. The bioassay results showed that title compound possesses broad spectrum of anticancer activity. The mechanism of action of 4-(4-fluorophenyl)amino-5,6,7-trimethoxyquinazoline against tumor cells was studied in androgen-independent prostate cancer PC-3 cells through microscopic observation, LDH release assay, and Western blot. It was found that title compound executed antitumor activity in a dose-dependent manner with 13±1.43 μM IC50 of 72 h treatment. Microscopic observation and LDH release assay revealed that the compound exe... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5421952 Fri, 11 Nov 2011 05:00:00 +0100 5421952 http://www.medworm.com/index.php?rid=5421951&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22073986%26dopt%3DAbstract Authors: Kim MJ, Woo JS, Kwon CH, Kim JH, Kim YK, Kim KH Abstract The flavonoid luteolin have been shown to exert anticancer activity in various cancer cells, however, the precise molecular mechanisms are not still completely understood. The present study was undertaken to determine the underlying mechanisms by which luteolin induces apoptosis in breast cancer cells. Luteolin induced a reduction in cell viability in a dose- and time-dependent manner. Pro-apoptotic effect of luteolin was demonstrated by cell cycle measurement and Hoechst 3325 staining. Western blot analysis revealed that luteolin caused activation of extracellular signal regulated kinase (ERK) and p38. Pharmacological inhibition or knockdown of ERK and p38 protected against the luteolin-induced cell death. The caspa... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5421951 Fri, 11 Nov 2011 05:00:00 +0100 5421951 http://www.medworm.com/index.php?rid=5421956&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22070356%26dopt%3DAbstract Authors: Leong PK, Chiu PY, Leung HY, Ko KM Abstract Schisandrin B (Sch B), the most abundant dibenzocyclooctadiene lignan in Fructus Schisandrae, has been shown to induce glutathione antioxidant and heat shock responses, as well as protect against oxidant-induced injury in various tissues, including that of the liver, in rodents and in AML12 hepatocytes. (-)Sch B was found to be the most potent stereoisomer of Sch B in producing cytoprotective action in AML12 hepatocytes. To define the role of reactive oxygen species (ROS) arising from cytochrome P-450 (CYP)-catalyzed metabolism of (-)Sch B in triggering glutathione antioxidant and heat shock responses, the effects of CYP inhibitor (aminobenzotriazole, ABT) and antioxidants (dimethylthiouracil, DTMU, and Trolox, TRX) on (-)Sch B-i... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5421956 Thu, 10 Nov 2011 05:00:00 +0100 5421956 http://www.medworm.com/index.php?rid=5421955&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22070397%26dopt%3DAbstract We examined the influence of bacterial ADP-ribosyltransferases C3-like exoenzymes, which selectively modify RhoA, B, and C proteins and inhibit signal pathways controlled by them. We then used RNA interference (siRNA Akt) and a PI3K inhibitor to analyze the changes caused by inhibiting the PI3K/Akt pathway. Inhibiting the activation of RhoA, B, C and Akt expression resulted in a decrease of the number of cells cultured in aggregates, and caspase 3 activation. RhoA activation and RhoB and RhoC expression were controlled by Akt, but RhoA expression was not. Inhibiting Akt and RhoA reduced the expression of a5 integrin, and inactivated FAK in B16F10 cells cultured as aggregates. Thus, inhibiting Rho subfamily proteins and Akt expression inactivates the FAK pathway and induces anoikis in anoik... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5421955 Thu, 10 Nov 2011 05:00:00 +0100 5421955 http://www.medworm.com/index.php?rid=5380243&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22050182%26dopt%3DAbstract In this study, we used primary cultured rat aortic VSMCs and specific antibodies for investigating the inhibitory mechanism of rosiglitazone on Ang II-induced VSMCs proliferation. We found that rosiglitazone treatment significantly inhibited Ang II-induced rat aortic VSMC proliferation in a dose-dependent manner. Western blot analysis showed that rosiglitazone significantly lowered phosphorylated ERK 1/2, Akt, mTOR, p70S6K, and 4EBP1 levels in Ang II-treated VSMCs. In addition, PPAR-g mRNA increased significantly and connective tissue growth factor (CTGF), fibronectin (Fn), and collagen III (Col III) levels decreased significantly. The present study demonstrates that the PPAR- g activator rosiglitazone can directly inhibit the pro-atherosclerotic effect of Ang II on rat aortic VSMCs. Rosig... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5380243 Fri, 04 Nov 2011 04:00:00 +0100 5380243 http://www.medworm.com/index.php?rid=5361581&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21999312%26dopt%3DAbstract Authors: Cerf ME Abstract The pancreas is characterized by a major component, an exocrine and ductal system involved in digestion, and a minor component, the endocrine islets represented by islet micro-organs that tightly regulate glucose homoeostasis. Pancreatic organogenesis is strictly co-ordinated by transcription factors that are expressed sequentially to yield functional islets capable of maintaining glucose homoeostasis. Angiogenesis and innervation complete islet development, equipping islets to respond to metabolic demands. Proper regulation of this triad of processes during development is critical for establishing functional islets. PMID: 21999312 [PubMed - in process] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361581 Tue, 01 Nov 2011 00:50:58 +0100 5361581 http://www.medworm.com/index.php?rid=5361580&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21999313%26dopt%3DAbstract Authors: Falus A, Marton I, Borbényi E, Tahy A, Karádi P, Aradi J, Stauder A, Kopp M Abstract As an outcome of The 2009 Nobel Prize in Physiology or Medicine, a connection has been highlighted between the length of telomeres and epigenetic effects, such as intensive changes in lifestyle and nutrition as well as behavioural and psychological factors. In this review, the various elements of molecular, cell biological, nutritional and lifestyle changes are introduced and discussed. PMID: 21999313 [PubMed - in process] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361580 Tue, 01 Nov 2011 00:50:17 +0100 5361580 http://www.medworm.com/index.php?rid=5361579&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21999314%26dopt%3DAbstract Authors: Wang W, Ma JL, Jia WD, Xu GL Abstract Despite advances in the development of anti-angiogenic agents for cancer treatment, the increase in the survival duration of cancer patients is still rather modest. One major obstacle in anti-angiogenic therapy is the emergence of drug resistance. Understanding the molecular mechanisms that enable a tumour to evade anti-angiogenic treatment is valuable to improve therapeutic efficacy. Targeting blood supply usually causes hypoxic responses of tumours that trigger a series of adaptive changes leading to a resistant phenotype. Periostin, a secreted ECM (extracellular matrix) protein, is mainly produced by CAFs (cancer-associated fibroblasts) on hypoxic stress. As CAFs have been casually linked to tumour resistance to angiogenesis blockad... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361579 Tue, 01 Nov 2011 00:49:09 +0100 5361579 http://www.medworm.com/index.php?rid=5361578&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21999315%26dopt%3DAbstract We report here that PGD2 induces A549 cell death through the intrinsic apoptotic pathway, although the process does not appear to involve either DP or CRTH2/DP2. Similar results were also found with H2199 cells, another type of NSCLC. We found that PGD2 metabolites induce apoptosis effectively and that 15d-PGJ2 (15-deoxy-Δ12,14-prostaglandin J2) is a likely candidate for the principal apoptotic inducer in PGD2-induced apoptosis in NSCLC A549 cells. PMID: 21999315 [PubMed - in process] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361578 Tue, 01 Nov 2011 00:48:22 +0100 5361578 http://www.medworm.com/index.php?rid=5361575&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22032345%26dopt%3DAbstract This study was conducted to further investigate the role of OPN in vSMC differentiation. Main methods: Primary cultures of aortic mouse vSMCs were transfected with an OPN expression plasmid and several vSMC differentiation markers including, α-smooth muscle actin (α-SM actin), SM22- α, tropomyosin and calponin were monitored in this cellular model. Key findings: α-SM actin and calponin protein levels were markedly decreased by OPN overexpression. Downregulation of α-SM actin and calponin was also observed upon extracellular treatment of mouse vSMCs with recombinant OPN. In addition, calponin mRNA was significantly decreased under serum-restricted conditions when OPN mRNA was dramatically increased, while α-SM actin mRNA remained unchanged. These data indicate that OPN downregulates �... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361575 Fri, 28 Oct 2011 04:00:00 +0100 5361575 http://www.medworm.com/index.php?rid=5361576&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22007870%26dopt%3DAbstract Authors: Kumar S, Saradhi M, Chaturvedi NK, Tyagi RK Abstract Observations made in live cells have clearly demonstrated that agonist-activated steroid/nuclear receptors reorganize in nucleoplasm into hundreds of discrete speckled structures commonly referred to as 'nuclear foci'. Subsequent studies have shown that 'nuclear foci' are formed only with agonist- and not with pure antagonist-bound receptors. Also, the other accessary components of transcriptional machinery co-localize in 'nuclear foci' with the activated receptors suggesting these to be active gene transcription sites. Recently, it has been observed that during mitosis 'nuclear foci' present in interphase of progenitor cells co-migrate with condensing chromatin and get inherited into the progeny cells. Ensuing events im... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361576 Tue, 18 Oct 2011 04:00:00 +0100 5361576 http://www.medworm.com/index.php?rid=5361577&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22003828%26dopt%3DAbstract In this study, we used a co-culture system for hematopoietic differentiation of mouse ESCs and induced pluripotent stem cells (iPSCs) in which the Wnt3a gene-modified OP9 cell line is used as stromal cells. We found that the number of CD41+ cells generated from both co-cultured mouse ESCs and mouse iPSCs increases significantly, which suggest that Wnt3a is involved in the early stages of hematopoietic differentiation of mouse ESCs and mouse iPSCs in vitro. PMID: 22003828 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361577 Mon, 17 Oct 2011 04:00:00 +0100 5361577 http://www.medworm.com/index.php?rid=5361583&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21988615%26dopt%3DAbstract Authors: Kulkeaw K, Inoue T, Mizuochi C, Horio Y, Ishihama Y, Sugiyama D Abstract Hmgn2, a ubiquitous nucleosome-binding protein that unfolds chromatin fibers and enhances DNA replication, reportedly regulates differentiation of epithelial and mesenchymal cells. To investigate how Hmgn2 regulates hematopoietic cell (HC) differentiation, we quantified Hmgn2 expression in HCs of mouse fetal liver (FL) during erythroid differentiation. Hmgn2 expression levels were over 10-fold higher in immature erythroid progenitors than in mature erythroid cells, suggesting that Hmgn2 antagonizes erythroid differentiation. To address this issue, we transfected Hmgn2 into both Friend erythroleukemia cells and into FL HCs. We observed a 3.3-fold decrease in relatively mature c-Kit+/CD71+ erythroid cel... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361583 Wed, 12 Oct 2011 04:00:00 +0100 5361583 http://www.medworm.com/index.php?rid=5361582&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21988655%26dopt%3DAbstract In conclusion, telomerization of hUCMSCs by hTERT overexpression extends their replicative life span without influencing hepatogenic differentiation potential and it offers opportunities for obtaining enough number of cells and for liver-directed therapies. PMID: 21988655 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361582 Wed, 12 Oct 2011 04:00:00 +0100 5361582 http://www.medworm.com/index.php?rid=5361587&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21980955%26dopt%3DAbstract Authors: Rowlands DK, Cui YG, So SC, Tsang LL, Chung YW, Chan HC Abstract Dysmenorrhoea, defined as cramping pain in the lower abdomen occurring before or during menstruation, effects up to 90% of women of childbearing age to varying degrees. The present study investigates whether Bak Foong Pills (BFP), a traditional Chinese medicine treatment for dysmenorrhoea, possesses analgesic properties. Results showed that BFP was able so significantly reduce pain responses following sub chronic treatment for 3 days, but not following acute (1 hour) treatment in response to acetic acid-induced writhing challenge in C57/B6 mice. The analgesic effect was not due to inhibition of COX activity as evidenced by the lack of inhibition of prostacyclin and prostaglandin E2 production by BFP. Molecula... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361587 Mon, 10 Oct 2011 04:00:00 +0100 5361587 http://www.medworm.com/index.php?rid=5361586&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21980981%26dopt%3DAbstract Authors: Yap YH, Say YH Abstract Since the discovery of cellular prion protein (PrPC), most studies have focused on its role in neurodegenerative diseases, whereas its function outside the nervous system remains obscure. We investigated the ability of PrPC in resisting tumour necrosis factor-α (TNF-α) apoptosis in three PrPC-transiently transfected cancer cell lines - renal adenocarcinoma ACHN, oral squamous cell carcinoma HSC-2 and colon adenocarcinoma LS174T. PrPC-expressing ACHN and LS174T cells had higher cell viabilities compared to the mock-transfected cells, while the transient over-expression of PrPC had minimal overall effect on HSC-2 cells due to its high endogenous PrPC expression. Cell cycle analysis was also performed, with both PrPC-expressing ACHN and LS174T cells ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361586 Mon, 10 Oct 2011 04:00:00 +0100 5361586 http://www.medworm.com/index.php?rid=5361585&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21981014%26dopt%3DAbstract In this study, it was demonstrated that perforin was recaptured into early endosome of NK cells via a clathrin-dependent endocytosis after target cells stimulation. Inhibition of clathrin-dependent endocytosis significantly attenuated the cytotoxicity of NK cells. These data suggest that the recovery of perforin contributes to the cytotoxicity of NK cells. The assay of endocytosis of lytic molecule would present a particular point to explore the mechanism of abnormal cytotoxicity of NK cells. PMID: 21981014 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361585 Mon, 10 Oct 2011 04:00:00 +0100 5361585 http://www.medworm.com/index.php?rid=5361584&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21981042%26dopt%3DAbstract Authors: Baohua Y, Li L Abstract SIRT6 (Sirtuin 6) has been identified as an anti-aging protein, and SIRT6-deficient mice display numerous progeroid and aging-like phenotypes. However, there is no report on the correlation between skin aging and SIRT6 in the literature. To explore how SIRT6 affects collagen metabolism and to further determine its role in skin aging, the levels of COL1A1 (type I collagen), COL3A1(type III collagen), and TGF-β1 (Transforming growth factor β1) mRNA expression were detected by RT-PCR (real-time quantitative polymerase chain reaction ), and the MMP1(matrix metalloproteinase-1) concentration in HDF (human dermal fibroblast ) supernate was determined by ELISA (enzyme-linked immunosorbent assay ) after blocking SIRT6. Compared with that of the control gr... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5361584 Mon, 10 Oct 2011 04:00:00 +0100 5361584 http://www.medworm.com/index.php?rid=5298174&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21967643%26dopt%3DAbstract In conclusion, HUVECs are damaged by overexpression but not by downregulation of PHP suggesting a pronounced impact of the enzyme on the cells when its activity is increased. PMID: 21967643 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5298174 Tue, 04 Oct 2011 04:00:00 +0100 5298174 http://www.medworm.com/index.php?rid=5298169&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21970403%26dopt%3DAbstract In this study, we have fully characterized this B1-559 region and found that it includes a B1 element, several repeats of ATG initiation codons and consensus DNA binding sites for erythroid-specific transcription factors NF-E2, GATA-1 and EKLF. Fragments derived from B1-559 incubated with nuclear extracts were found to form nuclear protein complexes in both undifferentiated and differentiated MEL cells. Furthermore, transient reporter-gene experiments in MEL and human erythroleukemia K-562 cells with recombinant constructs containing B1-559 fragments linked to HS-2 sequences of human β-globin gene LCR indicated potential cooperation upon erythropoiesis and globin gene expression. The possible interaction between the B1-559 region and βmajor globin gene transcriptional activation upon exe... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5298169 Tue, 04 Oct 2011 04:00:00 +0100 5298169 http://www.medworm.com/index.php?rid=5298168&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21970435%26dopt%3DAbstract Authors: Kawaguchi T, Hatano R, Yamaguchi K, Nawa K, Hashimoto R, Yokota H Abstract We investigated proplatelet formation (PPF) in the human megakaryocytic cell line UT-7/TPO in vitro and signal transduction pathways responsible for PPF. The megakaryocytic cell lines are a useful tool to study megakaryocyte biology, although PPF is induced only in the presence of phorbol ester. Thrombopoietin (TPO) stimulates megakaryocyte proliferation and differentiation, however, no PPF occurred in the megakaryocytic cell lines even after the addition of TPO. Therefore, factors other than TPO are believed to play an important role in the process of PPF. As PPF occurred in the bone marrow in vivo, we noted extracellular matrix proteins and found that soluble fibronectin (FN) induced potent PPF in... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5298168 Tue, 04 Oct 2011 04:00:00 +0100 5298168 http://www.medworm.com/index.php?rid=5298176&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21966886%26dopt%3DAbstract Authors: Qiu B, Hu JN, Liu R, Fan YW, Li J, Li Y, Deng ZY Abstract Although trans fatty acids (TFAs) are observed to have effects on many endothelial function, systemic inflammation and immune disorders, only limited experimental evidence is provided that TFAs participate in the pathogenesis of endothelial cell apoptosis. HUVECs grew in media supplied with elaidic acid (9t-C18:1) at 50, 100, 200, 400 µmol/l for 24 h. The detection of apoptosis were measured with flow cytometry analysis, the caspase-3, -8, -9 enzyme activities of HUVECs with colorimetric assay, the caspase-3, -8, -9 mRNA expression of HUVECs with RT-PCR. Results showed that 9t-C18:1 induced the apoptosis of HUVECs in a dose-dependent manner.The activities and mRNA expression of caspase-8, -9, and -3 were significa... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5298176 Mon, 03 Oct 2011 04:00:00 +0100 5298176 http://www.medworm.com/index.php?rid=5298175&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21966929%26dopt%3DAbstract Conclusions. Early differentiation of MSCs to progenitor oval cells and hepatocytes results in various therapeutic effects, including repair of damaged hepatocytes, intracellular glycogen restoration and resolution of fibrosis. Thus, these results support that in vivo hepatogenic differentiation process of MSC is related to the beneficial effects of MSC rather than the differentiated hepatocytes themselves. PMID: 21966929 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5298175 Mon, 03 Oct 2011 04:00:00 +0100 5298175 http://www.medworm.com/index.php?rid=5261474&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21933149%26dopt%3DAbstract Authors: Simon T, László V, Falus A Abstract A rapidly growing body of evidence highlighted that histamine, a small biogenic amine, is implicated in the regulation of DC (dendritic cell) functions. It is well established that DCs represent the most potent antigen-presenting cells of the body, linking innate and acquired immunity and regulating the outcome of immune responses. Signals, associated with ongoing inflammation and uptake of foreign antigens, promote maturation of DCs and activation of T-cell responses in secondary lymphatic organs. These bone marrow-derived cells patrol continuously all over the body. During their persistent migration, several mediators may influence the behaviour and functions of DCs. Histamine, produced by mast cells, basophils or DCs themselves, may... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261474 Thu, 29 Sep 2011 07:05:02 +0100 5261474 http://www.medworm.com/index.php?rid=5261473&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21933150%26dopt%3DAbstract Authors: Pei XH, Yang Z, Liu HX, Qiao SS Abstract The aim of the present study was to determine the effects of ARHI (aplasia Ras homologue member I; also known as DIRAS3), a member of the Ras superfamily, on HCC (hepatocellular carcinoma) cells and to define the molecular pathways involved. Stable transfection of ARHI into the HCC cell line Hep3B that lacks expression of this gene reduced cell proliferation significantly as compared with the transfection of empty vector (P<0.01). Moreover, the re-expression of ARHI induced significant apoptosis, whereas a few vector transfectants or non-transfected cells displayed apoptosis. Mechanistically, ARHI restoration impeded the activation of both Akt (also called protein kinase B) and NF-κB (nuclear factor κB). In vivo, restoring ARHI... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261473 Thu, 29 Sep 2011 07:05:02 +0100 5261473 http://www.medworm.com/index.php?rid=5261472&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21933151%26dopt%3DAbstract Authors: Li L, Xia Y, Wang Z, Cao X, Da Z, Guo G, Qian J, Liu X, Fan Y, Sun L, Sang A, Gu Z PMID: 21933151 [PubMed - in process] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261472 Thu, 29 Sep 2011 07:05:02 +0100 5261472 http://www.medworm.com/index.php?rid=5261470&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21936851%26dopt%3DAbstract In conclusion, the cumulative data indicated that this cellular therapy shows a significant potential for clinical use in the treatment of lameness associated with osteoarthritis. Considering that cellular therapy showed substantial promise in the treatment of osteoarthritis it should be applied before the injury becomes severe. PMID: 21936851 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261470 Thu, 22 Sep 2011 04:00:00 +0100 5261470 http://www.medworm.com/index.php?rid=5261469&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21939437%26dopt%3DAbstract Authors: Guo C, He Z, Wen L, Zhu L, Lu Y, Deng S, Yang Y, Wei Q, Yuan H Abstract There was an outbreak of urinary stones associated with consumption of melamine-tainted milk products in 2008 in China, leading to serious illness of many infants and even death. However, the toxicity of melamine in kidney epithelial cells remains unclear. Here we explored the effects of melamine and trolox on renal NRK-52e cells. Half inhibition concentration of melamine was measured by the MTT assay. Total superoxide dismutase (SOD) was determined by NBT staining method. Glutathione peroxidase (GSH-Px) activity was detected by UV colorimetric assay, and malondialdehyde (MDA) content was determined by thiobarbituric acid assay. The apoptosis induced by melamine was detected by flow cytometry. The IC50... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261469 Thu, 22 Sep 2011 04:00:00 +0100 5261469 http://www.medworm.com/index.php?rid=5261471&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21933154%26dopt%3DAbstract Authors: Yau TW, Kuchel RP, Koh JM, Szekely D, Mirtschin PJ, Kuchel PW Abstract Red blood cells (RBCs) circulate through narrow blood capillaries withstanding major deformations. The mechanical and chemical stresses commonly exerted on RBCs continue to attract interest for the study of membrane structure and function. Snake venoms are lethal biochemical "cocktails" often containing haemotoxins, metalloproteinases, myotoxins, neurotoxins, phosphodiesterases, phospholipases, and proteases. In our study, we monitored the effects of four snake venoms (Pseudechis guttatus, Oxyuranus scutellatus, Notechis scutatus, and Naja kaouthia) on human RBCs, using nuclear magnetic resonance (NMR) spectroscopy, differential interference contrast (DIC), and confocal light microscopy. The RBCs underw... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261471 Wed, 21 Sep 2011 04:00:00 +0100 5261471 http://www.medworm.com/index.php?rid=5261477&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929509%26dopt%3DAbstract Authors: Semsei AF, Erdelyi DJ, Ungvari I, Csagoly E, Hegyi MZ, Kiszel PS, Lautner-Csorba O, Szabolcs J, Masat P, Fekete G, Falus A, Szalai C, Kovacs GT Abstract Anthracyclines are potent cytostatic drugs the correct dosage of which is critical to aviod possible cardiac side effects. ABCC1 (MRP1) is expressed in the heart and takes part in the detoxification and protection of the cells from toxic effects of xenobiotics, including anthracyclines. Our objective was to search for associations between left ventricular function and single nucleotide polymorphisms of the ABCC1 gene in children who received anthracycline chemotherapy. We analyzed data of 235 pediatric patients with acute lymphoblastic leukemia. Patients were followed up by echocardiography (median follow up 6.3 years). Ni... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261477 Tue, 20 Sep 2011 04:00:00 +0100 5261477 http://www.medworm.com/index.php?rid=5261476&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929510%26dopt%3DAbstract Authors: Li T, Wang L, Ke XX, Hao XW, Xu M, Cui ZB, Cui H Abstract Neuroblastoma is one of the most common solid tumors in children, and comprises about 8-10% of all childhood malignancies. More than 22% cases have N-myc amplification which is associated with aggressively growing neuroblastomas. Oncogene-induced sensitization of cells to apoptosis is an important mechanism for suppression of tumorigenesis. Tumor suppressors often play a critical role in linking oncogenes to apoptotic machinery. For example, activated p53 then targets both intrinsic and extrinsic pathways to promote apo­ptosis through transcription-dependent and -independent mechanisms. Understanding of the involved mechanisms has important clinical implications. In here, we employed DNA damaging drug-induce... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261476 Tue, 20 Sep 2011 04:00:00 +0100 5261476 http://www.medworm.com/index.php?rid=5261475&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929512%26dopt%3DAbstract Authors: Hoz L, Romo E, Zeichner-David M, Sanz M, Nuñez J, Gaitán L, Mercado G, Arzate H Abstract ABSTRACT Periodontal ligament is a source of multipotent stem cells in humans and their differentiation potential to a cementoblast and osteoblast phenotypes has been shown. Tissue construction from periodontal ligament-derived cells (HPLCs) could be considered as a valuable technique for periodontal regenerative medicine. On these basis, we determined the role of CEMP1 as a factor to induce differentiation of HPLCs in a three dimensional (3D) fashion. HPLCs were grown in a RCCS D-410 rotating wall vessel (RWV) bioreactor, and maintained in either, experimental (CEMP1 2.5 µg/mL) or control media during 4 wk. Cell proliferation in the presence of CEMP1 was determined. The tissue-like... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261475 Tue, 20 Sep 2011 04:00:00 +0100 5261475 http://www.medworm.com/index.php?rid=5261478&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21923636%26dopt%3DAbstract Authors: Jurewicz E, Kasacka I, Bankowski E, Filipek A Abstract S100A6, a calcium binding protein also known as calcyclin, was detected in human umbilical cord by immunoblotting. Immunohistochemical studies showed an intensive immunoreaction for S100A6 in the walls of vessels and in Wharton's jelly. In the latter, S100A6 was found not only in the myofibroblasts but also in the extracellular matrix which surrounds these cells. Affinity chromatography performed on S100A6 resin indicated that Wharton's jelly contains some proteins which could bind to S100A6. Thus our results show for the first time the presence of S100A6 in umbilical cord and suggest the involvement of this protein in intra- and extracellular signaling pathways in this tissue. PMID: 21923636 [PubMed - as supplied ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5261478 Mon, 19 Sep 2011 04:00:00 +0100 5261478 http://www.medworm.com/index.php?rid=5231773&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21919889%26dopt%3DAbstract Authors: Sun Z, Wang Y, Gong X, Su H, Han X Abstract Bone marrow mesenchymal stem cells (MSCs) have been proved to differentiate into various tissue cells, including epithelial cells. This presents exciting possibilities for cellular therapy; however, the differentiation efficiency of MSCs is very low. Our aim is to explore some specific inducing factors to improve the epithelial differentiation efficiency of MSCs. Under induced conditions using RTE (rat tracheal epithelial) cell secretions, MSCs differentiated into epithelial cells and expressed several airway epithelial markers. We first used a rat cytokine antibody array to detect cytokines of the RTE secretion components and found that RTE cells could secrete 32 kinds of proteins. We later detected the concentration of seven pr... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5231773 Fri, 16 Sep 2011 04:00:00 +0100 5231773 http://www.medworm.com/index.php?rid=5231772&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21919890%26dopt%3DAbstract Authors: Fuchs R, Stelzer I, Drees CM, Rehnolt C, Schraml E, Sadjak A, Schwinger W Abstract Mesenchymal stem cells (MSCs) are currently in the spotlight of regenerative medicine to compete age dependent alterations of the human organism. Unfortunately, MSCs are affected by replicative senescence which is associated with decreasing proliferation potential, telomere shortening and DNA-damage during in vitro propagation. For monitoring of in vitro senescence of MSCs we assessed the integrity of DNA by means of the alkaline comet assay. For optimisation of the comet assay we have enhanced the stability of comet slides in liquid and minimised the background noise of the method by improving adhesion of agarose gels on the comet slides and concentrating cells on a defined small area on th... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5231772 Fri, 16 Sep 2011 04:00:00 +0100 5231772 http://www.medworm.com/index.php?rid=5231774&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21916848%26dopt%3DAbstract In conclusion, CSCs from human primary GBMs show different phenotypes and expressCD133, though at varying levels. However none of these parameters is directly correlated with the tumorigenic potentials of the cells. PMID: 21916848 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5231774 Thu, 15 Sep 2011 04:00:00 +0100 5231774 http://www.medworm.com/index.php?rid=5231775&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21916845%26dopt%3DAbstract This study shows diploid sub-cell inheritance of endopolyploid-division traits: perpendicular division relative to the cytoskeleton axis, dysfunctional centromere/kinetochore regions, and whole genomic separations from co-segregation. The assimilation of these traits into the innate mitotic machinery of the sub-cells resulted in diploid mitotic divisions which tolerated mild disturbances in cycling progression and in chromosomal distributions. These data were interpreted to demonstrate a blending together of endopolyploid- and mitotic division traits with result of an endo-modified mitosis in sub-cell-propagation. Additionally, chromosomal stickiness caused breakage in ana-telophase. These observations are discussed in regard to a potential for slowly developing aneuploidy with increasing ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5231775 Wed, 14 Sep 2011 04:00:00 +0100 5231775 http://www.medworm.com/index.php?rid=5231776&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21913891%26dopt%3DAbstract Authors: Bechyne I, Szpak K, Madeja Z, Czyz J Abstract The morphological and functional heterogeneity of solid tumour cells can be observed in cancer cell lines cultured in vitro. Here, we combined analyses of microclones developed from single cells with micropore transmigration assays to demonstrate the co-existence of cellular subsets differing in morphology, motile activity as well as Cx43 and N-cadherin expression within lung carcinoma A549 populations. "Fibroblastoid" cells, characterized by high motility, polarized morphology and plasmalemmal localization of Cx43 displayed the strongest aptitude for transmigration through narrow obstacles. Due to high mitotic activity, they maintain the whole population but can also give rise to a subpopulation of quiescent and immobile "epit... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5231776 Tue, 13 Sep 2011 04:00:00 +0100 5231776 http://www.medworm.com/index.php?rid=5219306&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21910691%26dopt%3DAbstract In this study, the MSCs derived from healthy human bone marrow samples were cultured and characterized by immunophenotyping and differentiation potential into osteoblast and adipocytes. Transdifferentiation into hepatocyte-like cells was performed in the presence and the absence of IGF-I in combination with predefined hepatic differentiation cocktail. To evaluate transdifferentiation, morphological features, immuno-cytochemical staining of specific biological markers, and hepatic functions were assessed. Morphological assessment and evaluation of glycogen content, albumin and α-feto protein expression as well as albumin and urea secretion revealed statistically significant difference between experimental groups compared to the control. Morphology and function (albumin secretion) of IGF-I-... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219306 Tue, 13 Sep 2011 04:00:00 +0100 5219306 http://www.medworm.com/index.php?rid=5219307&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21906030%26dopt%3DAbstract Authors: Feng F, Yang J, Tong L, Yuan S, Tian Y, Hong L, Wang W, Zhang H Abstract Dendritic cells (DCs) are the strongest professional antigen presenting cells to initiate immune responses against pathogens, but they are usually incompetent to initiate efficient immune responses in the progress of solid tumors. Here we showed that the Notch signaling plays a pivotal role in the DC-dependent anti-tumor immunity. Compared with the control DCs, the OP9-DL1 cells co-cultured DCs showed increased tumor suppression activity when inoculated together with tumor cells. This was most likely due to the activation of Notch signaling in DCs which enhanced their ability to evoke anti-tumor immune responses in solid tumors. Indeed, the OP9-DL1 cells co-cultured DCs expressed higher level of MHC I... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219307 Mon, 12 Sep 2011 04:00:00 +0100 5219307 http://www.medworm.com/index.php?rid=5219309&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21902673%26dopt%3DAbstract Authors: Kim H, Sakamoto K Abstract Tea catechin, (-) epigallocatechin gallate (EGCG), is one of the compounds that have been reported to act against obesity and diabetes. In order to determine the effect of EGCG on adipocyte differentiation, we treated 3T3-L1 preadipocytes with different kinds of catechins. Oil Red O staining showed that catechins, especially EGCG, significantly reduced intracellular lipid accumulation. Cell cycle analysis revealed that EGCG inhibited cell proliferation by disturbing the cell cycle during the clonal expansion of 3T3-L1. Real-time polymerase chain reaction (PCR) revealed that EGCG noticeably reduced mRNA expression of peroxisome proliferator-activated receptor gamma (PPARg), CCAAT/enhancer binding protein alpha (C/EBPα), and forkhead box class O (... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219309 Fri, 09 Sep 2011 04:00:00 +0100 5219309 http://www.medworm.com/index.php?rid=5219308&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21906028%26dopt%3DAbstract In this study we analyzed some aspects of cell cycle control and amino acid transport in hypertonic conditions during prenatal life. The effects of hyperosmotic stress on aminoacid uptake mediated by system A and on 3H-thymidine incorporation, and on the regulation of cell cycle proteins were analyzed in chick embryo hepatocytes. Hypertonic stress was able to increase system A activity and to cause cell cycle delay. Effects on amino acid transport involved p38 kinase activation and new carrier synthesis. Cyclin D1, cdk4 and PCNA levels decreased, while cyclin E, p21 and p53 levels were unchanged. Incorporation of 3H-leucine revealed a decreased synthesis of cyclin D1 protein. In contrast, the analysis of mRNA by qRT-PCR showed a net increase of cyclin D1 transcript, suggesting post-transcr... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219308 Fri, 09 Sep 2011 04:00:00 +0100 5219308 http://www.medworm.com/index.php?rid=5219310&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21899518%26dopt%3DAbstract Authors: Horbay RO, Manko BO, Manko VV, Lootsik Sr MD, Stoika R Abstract Respiration characteristics of mitochondria of the parental and giant cells of murine Nemeth-Kellner lymphoma (NK/Ly) were studied. The giant cell-enriched ascites was obtained by serial intraperitoneal injections of vinblastine in tumor-bearing mice. Ascites containing <70% giant cells were used. Their diameter of was over 17 µm (~2,800 µm3), while the diameter of the parental cells was 12.7 µm (1100 µm3). The respiration rate of mitochondria in situ was measured by oxygen consumption in intact and digitonin-permeabilized NK/Ly cells. Endogenous respiration of intact giant NK/Ly cells was 3 x higher comparing to the parental ones, roughly in agreement with the volume change. The giant NK/Ly cells were ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219310 Thu, 08 Sep 2011 04:00:00 +0100 5219310 http://www.medworm.com/index.php?rid=5219311&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21899515%26dopt%3DAbstract Conclusions: We conclude that CPC seeded with pBMP-2 transfected bMSCs mediated by GenEscort™ II could enhance ectopic new bone formation in nude mice, suggesting that GenEscort™ II mediated pBMP-2 gene transfer is an effective nonviral method and CPC is a suitable scaffold for gene enhanced bone tissue engineering. PMID: 21899515 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219311 Wed, 07 Sep 2011 04:00:00 +0100 5219311 http://www.medworm.com/index.php?rid=5219313&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895605%26dopt%3DAbstract Authors: Yang L, Guan H, He J, Zeng L, Yuan Z, Xu M, Zhang W, Wu X, Guan J Abstract The aim of this study was to investigate whether vascular endothelial growth factor (VEGF) regulates the proliferative capacity and endothelial nitric oxide synthase/nitric oxide (eNOS/NO) pathway of endothelial progenitor cells (EPCs) by activating calcineurin/nuclear factor of activated T cells (CaN/NFAT) signalling. EPCs were obtained from cultured mononuclear cells isolated from the peripheral blood of healthy adults. Treatment with VEGF (50 ng/ml) potently promoted CaN enzymatic activity, the activation of NFAT2, cell proliferation, eNOS protein expression, and NO production. Pretreatment with cyclosporin A (10 μg/ml), a pharmacological inhibitor of CaN, or 11R-VIVIT, a special inhibitor of NF... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219313 Tue, 06 Sep 2011 04:00:00 +0100 5219313 http://www.medworm.com/index.php?rid=5219312&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21895606%26dopt%3DAbstract Authors: Zhang Y, Lai Y, Chen HQ, Liu YF Abstract Cardiovascular and cerebrovascular diseases remain the leading cause of death in the world. Atherosclerosis is not only an inflammatory disease in which chemokines play the main role, but also a disorder that is related to blood shear stress. We have investigated the action of interleukin-8 (IL-8) mRNA expression in human endothelial cells line-EA.Hy926 under shear stress at different intensities and duration. Expression increases with time in an intensity-dependent manner. With regard to the transcriptional mechanism involved, transient transfection of the human wild-type IL-8 promoter (-162/+44)/luciferase reporter plasmid, or site mutation of one of the binding sites (AP-1 or NF-κB) in the IL-8 promoter region was investigated. ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219312 Tue, 06 Sep 2011 04:00:00 +0100 5219312 http://www.medworm.com/index.php?rid=5219314&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21888623%26dopt%3DAbstract Authors: Yang C, Liu HZ, Fu ZX Abstract Abstract Cellualr uptake of PEG-liposomal oxaliplatin induces bioactive changes in colorectal cancer, thus we investigated the apoptotic effect and anticancer mechanism of PEG-liposomal oxaliplatin. We treated the human colorectal cancer SW480 cell line with PEG-liposomal oxaliplatin and Caspase-8 inhibitor (Z-IETD-FMK). We evaluated apoptosis using flow cytometry (FCM) and Terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick end labelling (TUNEL) assay. Expression of Fas/FasL and Cytochrome C was detected using FCM and Immunofluorescence assay. Expression of Caspase-8, Bid, Caspase-9, Caspase-7, and activated Caspase-3 (P17) was observed in the Western blot analyses. Our results indicated that PEG-liposomal oxaliplatin (containing ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219314 Fri, 02 Sep 2011 04:00:00 +0100 5219314 http://www.medworm.com/index.php?rid=5219315&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21883092%26dopt%3DAbstract This study concludes that serum supplementation is an important consideration when carrying out toxicological assessments of nanomaterials and further highlights the need for standardisation of in vitro testing methods. PMID: 21883092 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219315 Thu, 01 Sep 2011 04:00:00 +0100 5219315 http://www.medworm.com/index.php?rid=5219316&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21880014%26dopt%3DAbstract Authors: Mattioli M, Gloria A, Turriani M, Mauro A, Curini V, Russo V, Tetè S, Marchisio M, Pierdomenico L, Berardinelli P, Colosimo A, Muttini A, Valbonetti L, Barboni B Abstract This research has been designed to characterize stemness properties and osteogenic potential of sheep amniotic epithelial cells (AEC). AEC have been isolated from 3 month old fetuses and expanded in vitro for 12 successive passages. The morphology, surface markers, stemness markers and osteogenic differentiation have been investigated after 1, 6 and 12 passages of expansion. AEC clearly expressed the stemness markers Oct4, Nanog, Sox2 and TERT and displayed low levels of global DNA methylation. AEC could be rapidly expanded in vitro with an average doubling time in culture of 24h. The culture had moderat... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5219316 Wed, 31 Aug 2011 04:00:00 +0100 5219316 http://www.medworm.com/index.php?rid=5176809&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21864295%26dopt%3DAbstract Authors: Li Z, Zhang H, Yang J, Hao T, Li S Abstract The aberrant methylation of promoter CpG islands is known to be a major inactivation mechanism of tumor-related genes, which plays a crucial role in the progression of silencing in human cancers including hepatocellular carcinoma (HCC). Thus, we sought to examine the promoter methylation status of five important DNA damage response genes in fresh-frozen hepatocellular carcinoma tissues and cell lines, as well as the possible correlation between methylation patterns and clinical features of the hepatocellular carcinoma. Promoter methylation status of RASSF1A, CHFR, GSTP1, MGMT and hMLH1 were examined by the methylation-specific PCR in 70 hepatocellular carcinoma tissues and 5 hepatocellular carcinoma cell lines. The mRNA expressio... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5176809 Wed, 24 Aug 2011 23:00:00 +0100 5176809 http://www.medworm.com/index.php?rid=5157523&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21861846%26dopt%3DAbstract Authors: Jeon W, Jeon Y, Nam M Abstract Natural flavonoids are associated with anti-proliferation of cancer growth. However, the anti-oxidant and anti-proliferation effects of aloe-emodin (AE) have not been well studied. The aim of this study is to demonstrate how AE affects the proliferation of hepatic hepatocellular carcinoma cells and to understand the anti-cancer effect of AE. The cytotoxic effect of AE was demonstrated using an MTT assay and Huh-7 cells were inhibited by AE treatment in both dose- and time-dependent manners. The half-maximal inhibitory concentration (IC50) level of AE was approximately 75 μM. AE also has anti-proliferative effects via induction of DNA damage and apoptosis. 2-dimensional electrophoresis revealed that several proteins were related to the anti-c... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=5157523 Tue, 23 Aug 2011 23:00:00 +0100 5157523 http://www.medworm.com/index.php?rid=4962599&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21675961%26dopt%3DAbstract Conclusion NCTD inhibits the albumin-induced cell proliferation and FN expression in HK-2 cells, which may furtherly proof the antifibrotic role of NCDT in proteinuria associated tubulointerstitial damage. PMID: 21675961 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4962599 Tue, 14 Jun 2011 23:00:00 +0100 4962599 http://www.medworm.com/index.php?rid=4962600&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21663595%26dopt%3DAbstract Authors: Tan X, Jin G, Tian M, Qin J, Zhang L, Zhu H, Li H Fetal brain tissue can be used in cell replacement therapy for Parkinson's disease (PD), but there is a poor donor supply of this tissue. Neural stem cells (NSCs) may overcome this problem as they can be isolated and expanded in vitro. However, the usage of NSCs is limited because the differentiation of NSCs into specific dopaminergic neurons has proven difficult. In the present study, we investigated the effect of nuclear receptor related factor 1 (Nurr1), a transcription factor specific for the development and maintenance of the midbrain dopaminergic neurons on inducing the differentiation of NSCs into tyrosine hydroxylase (TH) immunoreactive dopaminergic neurons. Nonetheless, these cells exhibited an immature neuronal morpho... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4962600 Thu, 09 Jun 2011 23:00:00 +0100 4962600 http://www.medworm.com/index.php?rid=4912777&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21649585%26dopt%3DAbstract This report also presents water sorption findings that support the SHM. The SHM is applicable for cell systems in which it has been studied. In 7 cell systems examined, more than half to all of the cell water had properties unlike those of bulk water. The SHM predicts and explains the commonly cited and measured bound water fraction of 0.2 to 0.4 g water/g dry mass on proteins. The commonly accepted concept that water beyond this bound water fraction can be considered bulk-like water in its physical properties is unwarranted. PMID: 21649585 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4912777 Tue, 07 Jun 2011 23:00:00 +0100 4912777 http://www.medworm.com/index.php?rid=4912776&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21649586%26dopt%3DAbstract In this study, the effect of MSC on neutrophils in the presence of complete and serum deprived culture media was investigated. In the presence of MSC, the viability of neutrophils increase as measured in 24hrs of incubation at various supplementation of serum concentration. We have utilized Annexin V and PI staining to confirm whether the enhancement of neutrophil's viability is due to a reduction in programmed cell death. MSC significantly rescue neutrophils from apoptosis at 1%, 5% and 10% of foetal bovine serum supplementation. The fractions of viable and dead cells were increase and diminish respectively in the presence of MSC. Our data indicate MSC rescue neutrophils from nutrient or serum deprived cell death. However, whether this effect is exerted through a specific signalling pathw... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4912776 Tue, 07 Jun 2011 23:00:00 +0100 4912776 http://www.medworm.com/index.php?rid=4912775&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21649587%26dopt%3DAbstract Authors: Warner DR, Webb CL, Greene RM, Pisano MM Mice lacking the gene for folic acid receptor 1 (Folr1) have a neural tube defect that is rescued by maternal folate supplementation. Primary cultures of mouse embryonic fibroblasts (MEFs) were established from these embryos and the effect on various signaling pathways examined. TGFß-1 inhibited the proliferation of wild-type and Folr1-/- MEFs, and folate restriction, either in growth medium or through folate uptake, led to further inhibition of growth. This effect may be Smad-independent because reporter assays using the Smad dependent reporter, p3TP-lux, revealed attenuation of TGFß1/Smad signaling in Folr1-/- MEFs. Signaling through the canonical Wnt pathway measured by Wnt-3a stimulated expression of the target gene, Axin2, demons... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4912775 Tue, 07 Jun 2011 23:00:00 +0100 4912775 http://www.medworm.com/index.php?rid=4912779&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21635225%26dopt%3DAbstract Authors: Jugova A, Sustackova G, Legartova S, Stixova L, Kozubek S, Bartova E Here, we focused on epigenetic changes in leukemia and multiple myeloma (MM) cells. We show how the histone signature, DNA methylation, and levels of select tumor suppressor proteins can be affected by inhibitors of HDAC and Dnmts. Both inhibitors, Trichostatin A and 5-azacytidine, have ability to change histone signature by tumor-specific manner. In MM cells we observed changes in H3K4 methylation, while in leukemia cells, especially H3K9 methylation was affected by select inhibitors. Compared to normal peripheral blood lymphocytes, tumor cell samples were characterized by increased H3K9 acetylation, H3K4me2, H3K9me2, HP1a level and specific changes were also observed for DNA methylation. Additionally, we sh... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4912779 Thu, 02 Jun 2011 23:00:00 +0100 4912779 http://www.medworm.com/index.php?rid=4912778&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21639857%26dopt%3DAbstract The objective of this study was analyze the effect of dexamethasone on the biology of osteoblasts, together with its influence on the expression of ILK and b1 integrin. For this, primary cultures of human osteoblasts were exposed to dexamethasone at 10-9 M (physiological dose) and 10-6 M (pharmacological dose) for 24 and 48 h. Cell viability, apoptosis and cell adhesion were analyzed, as well as protein expression of β1 integrin and ILK. It was observed that cell viability and adhesion were reduced in the cultures evaluated. In comparison with the control cultures, there was slightly less apoptosis in the cultures exposed to the physiological dose and considerably more apoptosis in those exposed to the pharmacological dose. In all treated cultures, protein expression of ILK was slightly h... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4912778 Thu, 02 Jun 2011 23:00:00 +0100 4912778 http://www.medworm.com/index.php?rid=4912780&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21627586%26dopt%3DAbstract Authors: Cauchi RJ Gems or 'Gemini of Cajal bodies' are spherical nuclear aggregates of Survival Motor Neuron (SMN) complexes that frequently overlap Cajal bodies. Though described and characterised in mammalian tissues, gems were not reported in invertebrates. Stimulation of gem formation in the fruit fly Drosophila melanogaster was investigated through the constitutive overexpression of a fluorescently tagged transgene of DEAD-box SMN complex member Gemin3 in wild-type tissues. Though expression was predominantly cytoplasmic in the larval brain cells, Gemin3 was found enriched in multiple discrete bright foci in the nuclei of several tissues including epidermis, muscle and gut. Similar to their mammalian counterparts, Drosophila gems contained endogenous SMN and at times overlapped w... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4912780 Mon, 30 May 2011 23:00:00 +0100 4912780 http://www.medworm.com/index.php?rid=4912781&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21612579%26dopt%3DAbstract Authors: Jiang Sr Y, He B, Li NP, Ma J, Gong GZ, Zhang M Approximately 4% of patients experiencing chronic infection of human hepatitis C virus (HCV) ultimately develop HCC. The nonstructural protein 5A (NS5A) encoded by HCV has been reported to have an oncogenic role during HCV infection, but the precise mechanism remains largely unclear. The aim of this study is to investigate the signal transduction pathways that mediate the role of NS5A in hepatocarcinogenesis. HepG2 cells were transfected with a plasmid expressing HCV NS5A protein. Subsequently, cell proliferation was analyzed by MTT assay and cell counting, apoptosis was analyzed by Hochest33342 staining, and gene expression profile was identified by microarray and subsequently validated by RT-PCR. The protein levels of survivin,... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4912781 Wed, 25 May 2011 23:00:00 +0100 4912781 http://www.medworm.com/index.php?rid=4866069&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21605079%26dopt%3DAbstract In this study, we down-regulated MUC2 expression by RNA interference (RNAi) and investigated the in vitro and in vivo effects on the proliferation and invasion/migration potential of human LS174T colorectal cancer cells. The LS174T cell line is a goblet-cell-like colorectal cancer cell line that continuously produces high-levels of MUC2. Inhibition of MUC2 expression in vitro by transfection of LS174T cells with recombinant plasmid pcDNA6.2-GW/EmGFP-miR-MUC2 led to the production of a stably-transfected MUC2-RNAi LS174T cell line. The proliferation and invasion/migration of MUC2-RNAi cells in vitro were significantly higher than in control cells, as assessed by MTT, colony formation and transwell assays, respectively. Subcutaneous injection of MUC2-RNAi LS174T cells into nude mice resulted... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4866069 Mon, 23 May 2011 23:00:00 +0100 4866069 http://www.medworm.com/index.php?rid=4866068&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21605080%26dopt%3DAbstract In conclusion, this studies indicated that GdCl3 inhibited PC3 cell migration mediated by the inactivation of both ERK and p38 MAPK pathways via PTx-sensitive G proteins, but also suppressed PC3 cell-induced osteoclast differentiation via regulating the mRNA expression of OPG and RANKL. PMID: 21605080 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4866068 Mon, 23 May 2011 23:00:00 +0100 4866068 http://www.medworm.com/index.php?rid=4866067&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21605083%26dopt%3DAbstract Authors: Zheng YM, Zheng YL, He XY, He XN, Zhao X, Sai WJ We have isolated stem cells from amniotic fluid of goat at terminal gestational age and transferred enhanced green fluorescence protein (EGFP) gene into the stem cells before. The aims of this study were to determine whether the transgenic stem cells have the capability of multipotent differentiation. The transgenic stem cells were induced to differentiate into neurogenic, adipogenic, osteogenic and endothelial cells in vitro, respectively. Markers associated with amniotic fluid-derived stem (AFS) cells and the differentiated cells were test by RT-PCR. The results demonstrated that the transgenic AFS cell were capable of self-renewal, a defining property of stem cells. AFS cells were positive for the undifferentiated cell marker... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4866067 Mon, 23 May 2011 23:00:00 +0100 4866067 http://www.medworm.com/index.php?rid=4866070&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21599657%26dopt%3DAbstract Authors: Luo Y, Hu W, Xu R, Hou B, Zhang L, Zhang W ZNF580 (Gene ID: 51157), a novel gene encoding a Cys2-His2 (C2H2) zinc finger transcriptional factor, may be involved in the maintenance of vascular endothelium homeostasis. To investigate the physiological role of the transcriptional factor ZNF580, we screened human fetal brain cDNA library with a yeast two-hybrid system and identified fourteen proteins that interact with ZNF580. The interaction between ZNF580 and SMAD2 2was confirmed by co-immunoprecipitation. Co-localization between endogenous ZNF580 and SMAD2 was mainly found in the nuclei of EA.hy926 endothelial cells with immunofluorescence and confocal microscopy. Our results suggest that ZNF580 is a binding partner of SMAD2 and it is involved in the signal transduction of tran... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4866070 Sun, 22 May 2011 23:00:00 +0100 4866070 http://www.medworm.com/index.php?rid=4866071&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21592091%26dopt%3DAbstract Authors: Gala K, Burdzinska A, Idziak M, Makula J, Paczek L It is generally accepted, that autologous transfers as non-immunogenic, constitute the safest approach in cellular transplantations. However, this attitude is often associated with the need of isolation and extracorporeal propagation of cells derived from aged patients. Thus, the knowledge about relationship between aging and the properties of mesenchymal stem cells is crucial in developing new clinical strategies. The aim of this study was to perform complex comparison of MSC derived from young and aged individuals what included phenotype, proliferating rate, osteogenic and adipogenic potential and secretory activity. Evaluated populations were isolated from bone marrow of 3 months old and 24 months old rats. There was no sig... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4866071 Wed, 18 May 2011 23:00:00 +0100 4866071 http://www.medworm.com/index.php?rid=4866072&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21585339%26dopt%3DAbstract In this study, we cloned the VIL2 -1541/-706 segment and investigated its transcriptional regulatory properties via luciferase assays in transiently transfected human embryonic kidney (HEK) 293 cells. The VIL2 -1541/-706 was found to exhibit promoter activity. Furthermore, when this segment was located upstream of the VIL2 or simian virus 40 (SV40) promoters in the forward orientation, the expression levels of luciferase were dramatically enhanced. However, this transcriptional enhancement disappeared when this segment was located upstream of the promoter in the reverse orientation or downstream of the reporter gene in the forward or reverse orientation. In deletion experiments, we found several potential regulatory regions within the VIL2 -1541/-706. When these regions were separately loc... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4866072 Tue, 17 May 2011 23:00:00 +0100 4866072 http://www.medworm.com/index.php?rid=4866073&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21574962%26dopt%3DAbstract In this study we examined the effects of hypoxia and the potential role of the GTPase RhoA and hypoxia-inducible transcription factor HIF-1a on MSC migration. Our data demonstrate that hypoxia decreases MSC migration through a HIF-1a and RhoA-mediated pathway. The active GTP-bound form of RhoA was reduced in 1% oxygen, whereas activation of RhoA under hypoxic conditions rescued migration. Furthermore, stabilization of HIF-1a under normoxic conditions attenuated cell migration similar to that of hypoxia. These data suggest that hypoxia negatively affects MSC migration by regulating activation of GTPases. These data highlight the importance of oxygen in regulating the recruitment of progenitor cells to areas of ischemic tissue damage. PMID: 21574962 [PubMed - as supplied by publisher] (S... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4866073 Sun, 15 May 2011 23:00:00 +0100 4866073 http://www.medworm.com/index.php?rid=4866074&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21561436%26dopt%3DAbstract Authors: Mingxia Z, Jinyuan Z, Guian C, Li G Human embryonic stem cells (hESCs) can differentiate into tissue derivatives of all three germ layers in vitro and mimic the development of the embryo in vivo. In this article, we have investigated the potential of an hEHuman embryonic stem cells (hESCs) can differentiate into tissue derivatives of all three germ layers in vitro and mimic the development of the embryo in vivo. In this article, we have investigated the potential of an hESC-based assay for the detection of toxicity to cardiac differentiation in embryonic development. First of all, we developed the protocol of cardiac induction from hESCs according to our previous work and distinguished cardiac precursor cells and late mature cardiomyocytes from differentiated cells, demonstrat... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4866074 Wed, 11 May 2011 23:00:00 +0100 4866074 http://www.medworm.com/index.php?rid=4814518&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21557726%26dopt%3DAbstract Conclusion Down-regulation of c-myc expression can inhibit the invasion of the QBC939 cells in vitro. PMID: 21557726 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4814518 Tue, 10 May 2011 23:00:00 +0100 4814518 http://www.medworm.com/index.php?rid=4814517&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21557727%26dopt%3DAbstract This study reports the radiosensitizing effect of TQ on human breast adenocarcinoma cells MCF7 (wild type p53) and ductal carcinoma cells T47D (mutant p53). TQ in combination with single dose of radiation (2.5 Gy) was found to exert supra-additive cytotoxic effects on both the cells as measured by cell proliferation and colony formation assay. The IC50 (concentration required to reduce survival by 50%) values of TQ for MCF7 and T47D cells were ~104 and ~37 µM respectively, which was further reduced to ~70 and ~18 µM respectively, when cells were irradiated at 2.5 Gy. Annexin-V binding and flow cytometry analysis revealed the role of enhanced apoptosis and cell cycle modulation in the mechanism of TQ mediated radiosensitization. Present investigation demonstrated the role of TQ as an adju... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4814517 Tue, 10 May 2011 23:00:00 +0100 4814517 http://www.medworm.com/index.php?rid=4814520&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21542803%26dopt%3DAbstract In this study, we demonstrate that BMSCs can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment. BMSCs were co-cultured with MRC-5 cells in modified small airway growth medium. The BMSCs-derived type II alveolar epithelial cells morphologically resemble human lung epithelial cells. They began to appear after 10 days in co-culture and became morphologically dominant after day 15. Correspondingly, surfactant protein C (SPC), a specific functional marker of human type II alveolar epithelial cells, was detected in differentiated cells by reverse transcription polymerase chain reaction analysis after day 15. Immunostaining analysis revealed the present of scattered SPC positive cells with a differentiation efficiency of 2.43% to... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4814520 Tue, 03 May 2011 23:00:00 +0100 4814520 http://www.medworm.com/index.php?rid=4814519&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21542806%26dopt%3DAbstract This study supports the hypothesis that combining the inhibition of melanogenesis with PDT should be explored as a valid therapeutic target for the management of advanced melanoma. PMID: 21542806 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4814519 Tue, 03 May 2011 23:00:00 +0100 4814519 http://www.medworm.com/index.php?rid=4707797&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21476985%26dopt%3DAbstract Authors: Wheatley DN Although protein synthesis and protein degradation are two independent processes that are firmly regulated, how they maintain a balance of protein in the non-growing cell remains to be established. In work in the 1980s, the author suggested a self-regulating mechanism. However, experimental work on this interesting and fundamental problem is needed for a better understanding of 'protein balance' in cells. PMID: 21476985 [PubMed - in process] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4707797 Thu, 14 Apr 2011 00:15:25 +0100 4707797 http://www.medworm.com/index.php?rid=4707796&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21476986%26dopt%3DAbstract Authors: Yewdell JW, Lacsina JR, Rechsteiner MC, Nicchitta CV Protein degradation is a critical factor in controlling cellular protein abundance. Here, we compare classical methods for determining protein degradation rates to a novel GFP (green fluorescent protein) fusion protein based method that assesses the intrinsic stability of cloned cDNA library products by flow cytometry [Yen et al. (2008) Science 322, 918]. While no method is perfect, we conclude that chimeric gene reporter approaches, though powerful, should be applied cautiously, due principally to GFP (or other reporter tag) interference with protein organelle targeting or incorporation into macromolecular assemblies, both of which cause spuriously high degradation rates. PMID: 21476986 [PubMed - in process] (Source: Ce... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4707796 Thu, 14 Apr 2011 00:15:20 +0100 4707796 http://www.medworm.com/index.php?rid=4707795&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21476987%26dopt%3DAbstract Authors: Xu W, Hui C, Yu SS, Jing C, Chan HC CF (cystic fibrosis) is a recessive genetic disease caused by mutations of the CFTR (cystic fibrosis transmembrane conductance regulator), a cAMP-activated anion channel, exhibiting a multitude of clinical manifestations including lung inflammation/infection, pancreatic insufficiency/diabetes, intestinal obstruction and infertility in both sexes. While mutation DF508 is found in 70% of CF patients, large variation in disease phenotypes and severity is observed among the patients. This review discusses current theories accounting for the disease variations and puts forth an epigenetic hypothesis involving microRNAs. PMID: 21476987 [PubMed - in process] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4707795 Thu, 14 Apr 2011 00:15:15 +0100 4707795 http://www.medworm.com/index.php?rid=4707794&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21476989%26dopt%3DAbstract In this study, we investigated the effects of Gn on intracellular GTP pool size and its link to differentiation and apoptosis of K562 cells. It was found that Gn inhibited cell proliferation and induced G0/G1 cell cycle arrest in K562 cells following 24 h exposure to a single dose of Gn (1.5 μM), while no significant effects were observed on unstimulated and PHA-stimulated PBL cells at the Gn dose (1.5 µM) used. Based on the morphological changes, Wright-Giemsa staining, benzidine assay and the expression of cell surface markers (GPIIb and glycophorin A), as analyzed by flow cytometry, we found that K562 cells have differentiated toward megakaryocytic lineage. In addition, Gn induced apoptosis among K562 cells based on AO/EtBr and annexin-V/PI double staining observations. These changes,... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4707794 Sun, 10 Apr 2011 23:00:00 +0100 4707794 http://www.medworm.com/index.php?rid=4707798&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21449895%26dopt%3DAbstract Authors: Li L, Xia Y, Wang Z, Cao X, Da Z, Guo G, Qian J, Liu X, Fan Y, Sun L, Sang A, Gu Z Type 1 diabetes mellitus (T1DM) is an autoimmune disease characterized by T cell-mediated damage of islet β-cells. The pathology of non-obese diabetic (NOD) mice involves insulitis induced by T cell infiltration of the pancreas, similar to the pathogenic mechanism in T1DM patients. Bone marrow mesenchymal stem cells (BM-MSCs) are multipotent progenitors that can be isolated from a number of sources. Recent studies have shown that transplantation of MSCs into NOD mice can prevent insulitis and have therapeutic effects on T1DM. Here, we demonstrate that migration and adhesion of BM-MSCs from NOD mice were suppressed, in contrast to BM-MSCs from ICRmice. This suppression was accompanied by an abno... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4707798 Tue, 29 Mar 2011 23:00:00 +0100 4707798 http://www.medworm.com/index.php?rid=4652684&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21438858%26dopt%3DAbstract Authors: Mrówczyńska L, Salzer U, Iglič A, Hägerstrand H The composition of membrane rafts (cholesterol/sphingolipid-rich domains) cannot be fully deduced from analysis of detergent-resistant membranes fraction after solubilization in Triton X-100 at 4oC. It is hypothesized that the membrane curvature dependent lateral distribution of membrane components affect their solubilization. The stomatocytogenic, Triton X-100, cannot effectively solubilise membrane components, especially with regard to the outward membrane curvature. PMID: 21438858 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4652684 Fri, 25 Mar 2011 00:00:00 +0100 4652684 http://www.medworm.com/index.php?rid=4652685&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21426305%26dopt%3DAbstract Authors: Fanucchi S, Veale R Focal adhesion kinase (FAK)-mediated signalling reportedly suppresses caspase-8 activation, and as a consequence, rescues epithelial cells from Fas-mediated anoikis. Critical, was the use of a human oesophageal squamous carcinoma (HOSCC) cell line harboring mutant (mt) p53-R175H and displaying resistance to detachment and Tyr397 dephosphorylation of FAK. Here we show, although caspase-8 activation is delayed in the mt p53-R175H cell line, comparable apoptotic events evidenced in the wt p53 HOSCC cell lines could be induced in the mt p53-R175H cell line by strengthening the apoptotic stimulus. Significant to anoikis-related regulation, the delay in caspase-8 activation was accompanied by the maintenance of FAK Tyr397 phosphorylation, integrin β1-associated ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4652685 Wed, 23 Mar 2011 00:00:00 +0100 4652685 http://www.medworm.com/index.php?rid=4652688&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21418038%26dopt%3DAbstract Authors: Eckermann CW, Lehle K, Schmid SA, Wheatley DN, Kunz-Schughart LA Various assays of different complexity are applied in research on angiogenesis in health and disease. The results of these assays increasingly impact the field of tissue engineering because preformed microvascular networks may connect and conduct to the vascular system of the host, thereby helping to support the survival of implanted cells and tissue constructs. An interesting model that supports the formation of endothelial cell (EC) tubular structures in vitro is based on co-culturing them with fibroblasts (F). Our initial multilayer approach was recently transferred into a 3-dimensional spheroid model using HUVEC as model cells. Our current aim has been to further characterize, extend and validate this F/EC sp... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4652688 Mon, 21 Mar 2011 00:00:00 +0100 4652688 http://www.medworm.com/index.php?rid=4652687&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21418039%26dopt%3DAbstract In conclusion, Poly I:C and ATO combination is potentially a novel and effective approach for the treatment of HCC. PMID: 21418039 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4652687 Mon, 21 Mar 2011 00:00:00 +0100 4652687 http://www.medworm.com/index.php?rid=4652686&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21418040%26dopt%3DAbstract This study contributes information concerning the regulation and expression of molecules that promote chronic inflammatory processes leading to the emergence of periodontitis and consequent loss of the dental organ. PMID: 21418040 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4652686 Mon, 21 Mar 2011 00:00:00 +0100 4652686 http://www.medworm.com/index.php?rid=4652689&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21413929%26dopt%3DAbstract This study provides insight into host- chlamydia interaction at the cross-road of iron metabolism and immune responses. Extrapolation of these results may help in realizing the potential of modulators of iron homeostasis in treatment of chronic chlamydial infection. PMID: 21413929 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4652689 Thu, 17 Mar 2011 00:00:00 +0100 4652689 http://www.medworm.com/index.php?rid=4652690&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21410434%26dopt%3DAbstract In this study, C57BL/6 female mice were injected with pregnant mare serum gonadotropin (PMSG) to obtain ovarian granulosa cells (GCs), and then co-cultured with miPSCs. The morphological changes in the miPSCs co-cultured with GCs were observed by light microscopy. The expression of follicle stimulating hormone receptor (FSHR) was detected by immunocytochemistry and flow cytometry. Radioimmunoassay was used to analyze the level of estradiol (E2) in culture supernatants. The results showed that the proportion of GCs expressing FSHR in GCs was over 90%. The E2 concentration of the culture supernatant of the GCs group was 62.4 pg/ml on day 1, and decreased in a time-dependent manner. The opposite situation was observed in the miPSCs-GC co-cultured group with an E2 concentration of 87.9 pg/ml o... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4652690 Wed, 16 Mar 2011 00:00:00 +0100 4652690 http://www.medworm.com/index.php?rid=4652691&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21401527%26dopt%3DAbstract Chromosome "By-Aurora-ientation" during mitosis. Cell Biol Int. 2011 Mar 15; Authors: Wheatley SP New evidence from three separate laboratories, published recently in Science, has shown that centromere positioning of the chromosomal passenger complex (CPC) during early mitosis is achieved through direct interaction between the chromosomal passenger protein (CPP) survivin and histone H3. In essence an acidic pocket in the BIR domain of survivin binds to the NH2 tail of histone H3 specifically when it is phosphorylated at threonine 3, a mark that is placed by the mitotic kinase, haspin. These data are significant as they describe a fundamental mechanism, conserved throughout Eukaryotes, which is essential for chromosome biorientation and the maintenance of genome stability during... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4652691 Tue, 15 Mar 2011 00:00:00 +0100 4652691 http://www.medworm.com/index.php?rid=4584773&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391919%26dopt%3DAbstract Authors: Yan L, Yinghui T, Bo Y, Gang Z, Xian X, Lu Z The aim of this study was to investigate the in vitro effects and regulatory mechanism of calcitonin gene-related peptide (CGRP) on nitric oxide production (NO) in osteoblasts. Primary human mandibular osteoblasts (MOB) and osteoblast-like cells (MG-63) were either cultured with CGRP or co-incubated with inhibitors targeting endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), neuronal nitric oxide synthase (nNOS) and intracellular Ca2+ ([Ca2+]i). The NO concentration in cell culture supernatants was measured during the first 24 h using the Griess test; cellular NO was marked with the fluorescent marker DAF-FM DA and measured by fluorescence microscopy between 1-4 h after treatment. eNOS and iNOS mRNA ex... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584773 Fri, 11 Mar 2011 00:00:00 +0100 4584773 http://www.medworm.com/index.php?rid=4584772&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21395557%26dopt%3DAbstract Authors: Zhai L, Wang H, Tang W, Liu W, Hao S, Zeng X Condensin, a major non-histone protein complex on chromosomes, is responsible for the formation of rod-shaped chromosome in mitosis. A heterodimer composed of SMC2 (structural maintenance of chromosomes) and SMC4 subunits constitutes the core part of condensin. Although extensive studies have been done in yeast, fruit fly and Xenopus to uncover the mechanisms and molecular nature of SMC proteins, little is known about the complex in mammalian cells. We have conducted a series of experiments to unveil the nature of condensin complex in human chromsome formation. The results show that overexpression of the C-terminal domain of SMC subunits disturbs chromosome condensation, leading to formation of swollen chromosomes, while knockdown o... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584772 Fri, 11 Mar 2011 00:00:00 +0100 4584772 http://www.medworm.com/index.php?rid=4584771&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21395558%26dopt%3DAbstract In this study, small interfering RNAs (siRNAs) targeted against βig-h3 were transfected into SMMC-7721 cells [a human hepatocellular carcinoma (HCC) cell line] to knockdown the expression of βig-h3. We found that NiCl2, a potent blocker of extracellular Ca2+ entry, reduced βig-h3-induced secretion of MMP-2 and MMP-9. Further investigation suggested that reduction in the levels of βig-h3 decreased the secretion of MMP-2 and MMP-9 that was enhanced by an increase in the concentration of extracellular Ca2+. S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide (NO) donor, and 8-bromo-cGMP (8-Br-cGMP) inhibited thapsigargin-induced Ca2+ entry and MMP secretion in and the invasive potential of human SMMC-7721 cells. Further, the inhibitory effects of 8-Br-cGMP and SNAP could be significant... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584771 Fri, 11 Mar 2011 00:00:00 +0100 4584771 http://www.medworm.com/index.php?rid=4584775&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391915%26dopt%3DAbstract Authors: Bird SD, Legge M, Walker R Cellular thiols including glutathione (GSH) and L-cysteine (L-Cys) are essential for cell signaling, growth and differentiation. L-cysteine is derived from the extracellular thiol pool and is the rate limiting compound for intracellular GSH biosynthesis. The present study investigated the effect of thiol supplemented media on cell growth, phenotype and total GSH of cultured human peritoneal mesothelial cells (hPMC). Cells were cultured in medium M199 supplemented with 2% serum, with "plus" or without "minus" L-cysteine (L-Cys), and compared with medium supplemented with either b-mercaptoethanol (beta-ME), (0.25 mmol/L) or the receptor tyrosine kinase ligand epidermal growth factor (EGF, 100ng/mL). b-ME produced a disproportionate increase in total GS... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584775 Thu, 10 Mar 2011 00:00:00 +0100 4584775 http://www.medworm.com/index.php?rid=4584774&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21391916%26dopt%3DAbstract Authors: Zheng YM, Dang YH, Qiu S, Qi YP, Xu YP, Sai WJ The aims of this study were to 1) determine whether neural stem cells (NSCs) could be isolated from the brain of embryonic day 98 fetal goat, and to 2) determine if these stem cells have the capability of multipotent differentiation following transfection with a reporter gene, enhanced green fluorescence protein (EGFP), and to 3) study the characteristics of the stem cells cultured in attached and non-attached plates. NSCs were isolated from embryonic day 98 fetal goat brain, transfected with EGFP gene using lipofection, and cultured in attached and non-attached plates, respectively. The transgenic stem cells were induced to differentiate into osteogenic and endothelial cells in vitro, respectively. Markers associated with undiffe... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584774 Thu, 10 Mar 2011 00:00:00 +0100 4584774 http://www.medworm.com/index.php?rid=4584776&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21385157%26dopt%3DAbstract Authors: Mehdipour P, Kheirollahi M, Mehrazin M, Kamalian N, Atri M It was previously reported that tumor samples had shorter telomeres than the surrounding normal tissue. Hereby, the initial sign of correlation between malignant tissue and telomere behavior could be noticed. Bridging knowledge between germ- and somatic- cells could facilitate understanding cellular evolution. The aim of our investigation was to provide evidence for the evolutionary hypothesis of telomere length in primary breast cancer and brain tumors which might be applied as a prognostic and/or predictive marker. DNA extraction from the frozen tissues was performed using high pure PCR template preparation kit. Standard protocol of Telo TTAGGG Telomere Length Assay kit, a non-radioactive chemiluminescent assay, was ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584776 Tue, 08 Mar 2011 00:00:00 +0100 4584776 http://www.medworm.com/index.php?rid=4584777&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21375507%26dopt%3DAbstract In conclusion, a role of PARP-1 in protecting the testicular differentiation is suggested. PMID: 21375507 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584777 Fri, 04 Mar 2011 00:00:00 +0100 4584777 http://www.medworm.com/index.php?rid=4584779&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21355850%26dopt%3DAbstract Authors: Shoae-Hassani A, Sharif S, Verdi J Abstract Expression of four major reprogramming transgenes including Oct4, Sox2, Klf4 and cMyc in somatic cells enables them to have the pluripotency. These cells are induced pluripotent stem cells (iPSC) that currently show the greatest potential for differentiation into cells of the three germ lineages. One of the issues facing the successful reprogramming and clinical translation of iPS cell technology is the high rate of apoptosis after reprogramming process. Reprogramming is a stressful process and the p53 apoptotic pathway plays a negative role in cell growth and self renewal. Apoptosis via P53 pathway serves as a major barrier in nuclear somatic cell reprogramming during iPSC generation. Dehydroepiandrosterone (DHEA) is an abundant ste... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584779 Mon, 28 Feb 2011 00:00:00 +0100 4584779 http://www.medworm.com/index.php?rid=4584778&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21355853%26dopt%3DAbstract In conclusion our results raise the possibility that HSPs could regulate cell differentiation and proliferation under both physiological and pathological conditions. PMID: 21355853 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584778 Mon, 28 Feb 2011 00:00:00 +0100 4584778 http://www.medworm.com/index.php?rid=4584780&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21332449%26dopt%3DAbstract This study was conducted to determine if multi-walled carbon nanotubes (MWCNTs)-coated electrospun poly (L-lactic acid-co-3-caprolactone) (PLCL) nanofibers improved the neurite outgrowth of PC-12 cells. To accomplish this, two groups, PC-12 cells in either uncoated PLCL scaffolds or MWCNTs-coated PLCL scaffolds, were cultured for nine days. MWCNTs-coated PLCL scaffolds showed improved adhesion, proliferation and neurite outgrowth of PC-12 cells. These findings suggest that MWCNTs-coated nanofibrous scaffolds may be an attractive platform for cell transplantation application in neural tissue engineering. PMID: 21332449 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584780 Mon, 21 Feb 2011 00:00:00 +0100 4584780 http://www.medworm.com/index.php?rid=4584781&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21332445%26dopt%3DAbstract Authors: McGinn O, English W, Roberts S, Ager A, Newham P, Murphy G ADAMs (A Disintegrin And Metalloprotease) are a family of type I transmembrane glycoproteins related to snake venom metalloproteases and disintegrins. They are regulatory proteins that modulate intercellular adhesion and the bioavailability of growth factors and have been implicated in many disease states including cancer, immunity and inflammation. One member of the ADAM family, ADAM28, has been reported to bind to the integrin a4b1 in humans; however the distribution of ADAM28 and the biological consequences of ADAM28:a4b1 interactions are yet to be fully elucidated. The expression of ADAM28 in human and murine tissues was examined by multiple Affymetrix microarray analyses, real time RT-PCR and immunohistochemical s... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584781 Fri, 18 Feb 2011 00:00:00 +0100 4584781 http://www.medworm.com/index.php?rid=4584782&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21323641%26dopt%3DAbstract Authors: Tang R, Yang C, Tao JL, You YK, An N, Li SM, Wu HL, Liu HF Proteinuria is a common feature for almost all glomerular diseases, and reflects the severity of the glomerular lesion. The presence of large amount of proteins in tubular fluid, however, may also contribute to the development of renal interstitial fibrosis (RIF). Edocytosis of albumin in proximal tubular cells triggers protein kinase C (PKC)-dependent generation of reactive oxygen species, secretion of chemokines. As a family including 12 isozymes, which PKC isozymes participate in RIF is still unclear. Epthelial-mesenchymal transdifferentiation (EMT) of renal tubular epithelial cells (RTECs) plays a crucial role in the progress of RIF induced by proteinuria. In the present study, we investigated the role of classical... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584782 Wed, 16 Feb 2011 00:00:00 +0100 4584782 http://www.medworm.com/index.php?rid=4584783&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21320075%26dopt%3DAbstract Authors: Yuh IS Lysophosphatidic acid (LPA) is a bioactive phospholipid having diverse effects on various types of tissues. When normal murine mammary gland (NMuMG) cells were cultured in the presence of 0-10 μM LPA, cell numbers were increased by dose dependency for the 6-day culture periods (P<0.05). In DNA synthesis assay, 10 μM LPA induced 4.5 folds of DNA synthesis as much as control (P<0.05). In addition, the cultured cell density in the given area was increased by LPA treatment. Matrix metalloproteinase (MMP) inhibitor GM6001 and EGF receptor tyrosine kinase inhibitor AG1478 significantly decreased LPA-induced DNA synthesis and cell growth without cell death (P<0.05). To test the hypothesis that LPA-induced cell growth is mediated through LPA subtype receptors, LPA su... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584783 Tue, 15 Feb 2011 00:00:00 +0100 4584783 http://www.medworm.com/index.php?rid=4584786&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21314640%26dopt%3DAbstract Authors: Shen E, Zhao K, Wu C, Yang B CD4+CD25+Foxp3+ regulatory T cells (CD25+Treg cells) play a central role in the maintenance of peripheral self-tolerance and immune homeostasis. Previous study showed that CD25+Treg cells suppressed the differentiation and function of Th1 cells in vivo and in vitro. However, the mechanism of suppressing Th1 cell differentiation mediated by CD25+Treg cells remains unclear. In the present study, we found that CD25+Treg cells could reduce the production of IFN-γ and the percentage of IFN-γ-, IL-2-and TNF-α-producing cells by CD25-T cells under Th1 cell culture conditions, and suppress the differentiation of CD25-T cells into Th1 cells in a dose-dependent manner. Moreover, these CD25+Treg cells could inhibit the activation of CD25-T cells by down-re... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584786 Mon, 14 Feb 2011 00:00:00 +0100 4584786 http://www.medworm.com/index.php?rid=4584785&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21314641%26dopt%3DAbstract Authors: Jia X, Liu B, Shi X, Ye M, Zhang F, Liu H Silica is a potent occupational fibrogenic agent capable of inducing lung fibrosis and many other lung diseases. Our current study focused on the signaling pathways regulating cell cycle changes in human embryo lung fibroblast (HELF) after silica (α-quartz) exposure. Our results showed silica exposure could lead cell cycle changes. The cell cycle alternations were accompanied with over-expression of cyclin D1 and CDK4 in a time-dependent manner. Silica exposure also decreased E2F-4 expression in HELF. These changes were blocked by over-expression of dominant negative mutants of extracellular signal-regulated protein kinase (ERK) or the stress-activated c-Jun NH2-terminal kinase (JNK), respectively. Moreover, pretreatment of cells with... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584785 Mon, 14 Feb 2011 00:00:00 +0100 4584785 http://www.medworm.com/index.php?rid=4584784&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21314642%26dopt%3DAbstract In conclusion, we found the decreased PCDH10 expression in prostate cancer cells was associated with the aberrant methylation of PCDH10 promoter CGI. Our results may contribute to the understanding of the role of PCDH10 inactivation in the progression of prostate cancers. PMID: 21314642 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4584784 Mon, 14 Feb 2011 00:00:00 +0100 4584784 http://www.medworm.com/index.php?rid=4476409&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21309751%26dopt%3DAbstract This study investigates whether nucleolin is a membrane receptor for LPS during LPS-induced alveolar macrophages (AMs) activation. First, using immunofluorescence and microscopy, we detected the expression of nucleolin on the surface of AMs. Second, using LPS affinity columns, we demonstrated that nucleolin directly binds to LPS. Third, we found that LPS colocalized with nucleolin on both the cell surface and in the cytoplasm. Finally, knockdown of nucleolin expression on the cell surface using siRNA led to significant reductions in the internalization of LPS, in LPS-induced NF-κB-DNA binding and in the protein expression of tumor necrosis factor (TNF)-α and Interleukin-6 (IL-6). These findings provide evidences that cell-surface nucleolin on AMs may serves as a receptor for LPS and is e... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4476409 Fri, 11 Feb 2011 00:00:00 +0100 4476409 http://www.medworm.com/index.php?rid=4476410&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21306303%26dopt%3DAbstract Authors: Wang Q, Diao X, Sun J, Chen Z To investigate the mechanisms involved in prostate cancer (PCa) metastasis and chemokine receptor-4 (CXCR4)-mediated vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) expression, we used lentivirus-mediated RNA interference (RNAi) to reduce the expression of CXCR4 in a PCa cell line. We found that the silencing of CXCR4 led to a significant downregulation of VEGF and MMP-9 at both the mRNA and protein levels compared to the control in vitro. Using an animal model, we confirmed that CXCR4 silencing via subcutaneous injection could both reduce tumor growth and inhibit metastasis, particularly bone metastasis, of PCa. Using in vivo immunohistochemistry, we also found that the expressions of VEGF and MMP-9 were reduced b... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4476410 Thu, 10 Feb 2011 00:00:00 +0100 4476410 http://www.medworm.com/index.php?rid=4476411&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21299496%26dopt%3DAbstract Authors: Zybina Snr TG, Stein Sr GI, Zybina Snr EV The presence of keratin intermediate filaments is a characteristic of trophoblast differentiation. Meantime, their intracellular localization in the functionally different subtypes of placental trophoblast is poorly investigated in rodent whereas their placentae are being broadly investigated in recent years as a model of the feto-maternal interaction. The purpose was to study the intracellular distribution of cytokeratin filaments in correlation with glycogen deposits, the both being important constituents of the trophoblast cells in rat placenta. Different rat trophoblast cell populations exhibited different patterns of cytokeratin immunolocalization. The most intensive immunostaining was observed in the highly endopolyploid secondar... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4476411 Mon, 07 Feb 2011 00:00:00 +0100 4476411 http://www.medworm.com/index.php?rid=4476412&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21284606%26dopt%3DAbstract Authors: Gardiner J, Overall R, Marc J The Arabidopsis thaliana genome encodes about 386 proteins with coiled-coil domains of at least 50 amino acids in length. In mammalian systems, many coiled-coil proteins are part of various cytoskeletal networks including intermediate filament protein, actin-binding proteins and microtubule-associated proteins. Immunological evidence suggests that some of these cytoskeletal proteins, such as lamins, keratins and tropomyosins, may be conserved in Arabidopsis. However, coiled-coil proteins are of low complexity and thus traditional sequence comparison algorithms such as BLAST may not detect homologies. Here we use the PROPSEARCH algorithm to detect putative coiled-coil cytoskeletal protein homologues in Arabidopsis. This approach reveals putative in... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4476412 Wed, 02 Feb 2011 00:00:00 +0100 4476412 http://www.medworm.com/index.php?rid=4411893&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21261597%26dopt%3DAbstract Authors: Gong W, Ma KZ, Liang X, Liu Y, Zhou Q, Zhang J, Chen H, Liu WW IFN-γ can effectively suppress tumors, but it has also been found to promote tumor progression. However, the underlying mechanisms by which it enhances malignancy have not been fully elucidated. By using a mouse model that expresses IFN-γ locally in muscle, we found that the growth potential of tumors was increased after a quick decrease of IFN-γ. Furthermore, the up-regulation of IRF-2 and down-regulation of IRF-1 was also found in the tumor cells. Along these lines, IFN-γ led to down-regulated expression of cyclinD1, Bcl-2, and Bcl-xL and up-regulated expression of p21WAF1 and Bax in tumor cells. Yet, the expression of these genes, as well as activation of ERK and NF-κB, was also reversed shortly after a dec... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411893 Mon, 24 Jan 2011 00:00:00 +0100 4411893 http://www.medworm.com/index.php?rid=4411894&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21250945%26dopt%3DAbstract Authors: Erenpreisa J, Salmina K, Huna A, Kosmacek EA, Cragg M, Ianzini F, Anisimov A 'Neosis' describes the process whereby p53 function-deficient tumour cells undergo self-renewal after genotoxic damage apparently via senescing endopolyploid tumour cells (ETC). We previously reported that autophagic digestion and extrusion of DNA occurs in ETC, and subsequently revealed that self-renewal transcription factors are also activated under these conditions. Here we further studied this phenomenon in a range of cell-lines after genotoxic damage induced by gamma-irradiation, etoposide or paclitaxel treatment. These experiments revealed that chromatin degradation by autophagy was compatible with continuing mitotic activity in ETC. While the actively polyploidising primary ETC produced early a... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411894 Fri, 21 Jan 2011 00:00:00 +0100 4411894 http://www.medworm.com/index.php?rid=4411896&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21247410%26dopt%3DAbstract Authors: Liu Q, Guan L, Huang B, Li WH, Su Q, Yu M, Xu XP, Luo T, Lin SC, Sun X, Chen M, Chen XG Adult peripheral blood-derived cells are able to differentiate into a variety of cell types, including nerve cells, liver-like cells, and epithelial cells. However, their differentiation into retina-like cells is controversial. In the present study, transdifferentiation potential of human adult peripheral blood mononuclear cells into retina-like cells and integration into the retina of mice were investigated. Freshly isolated adult peripheral blood mononuclear cells divided into two groups: cells in group I were cultured in neural stem cell medium, and cells in group II were exposed to conditioned medium from rat retinal tissue culture. After five days, several distinct cell morphologies we... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411896 Thu, 20 Jan 2011 00:00:00 +0100 4411896 http://www.medworm.com/index.php?rid=4411895&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21247411%26dopt%3DAbstract In this study, human glioma cell line U251 was transfected with plasmids containing U6 promoter-driven shRNAs against human FAK plasmid using liposome. The effects of FAK knockdown in U251 cells in vitro were analyzed by using flow cytometry and PI staining assays. Based on the encouraging in vitro results with FAK silencing, plasmids encoding FAK-targeted shRNA were encapsulated by DOTAP:Cholesterol (DOTAP:Chol) cationic liposome and injected via tail vein to evaluate its therapeutic efficiency on suppressing tumor growth in a human glioma xenograft model. Changes in tumor angiogenesis, apoptosis, and proliferation were assessed by using PCNA, CD34 immunostaining and TUNEL assays, respectively. The results indicated that DOTAP:Chol could efficiently deliver therapeutic plasmids systemical... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411895 Thu, 20 Jan 2011 00:00:00 +0100 4411895 http://www.medworm.com/index.php?rid=4411898&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21244366%26dopt%3DAbstract Authors: Li BY, Li XL, Gao HQ, Zhang JH, Cai Q, Cheng M, Lu M To investigate the effects of grape seed procyanidin B2 (GSPB2) on the apoptosis of human umbilical endothelial cells (HUVEC) induced by advanced glycation end products (AGEs). HUVEC were treated with AGEs (200 μg/ml) in the presence or absence of GSPB2 (2.5, 5.0 and 10.0μmol/L). Our findings showed that: 1) AGEs induced HUVEC apoptosis and upregulated the expression of caspase-3 activation and lactadherin, and reduced the phosphorylation of glycogen synthase kinase 3β (GSK3β) at baseline. 2) Treatment of HUVEC with GSPB2 significantly inhibited the cell apoptosis and the expression of caspase-3 activation and lactadherin induced by AGEs. Moreover, GSPB2 inhibited intracellular reactive oxygen species in a dose-dependent... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411898 Wed, 19 Jan 2011 00:00:00 +0100 4411898 http://www.medworm.com/index.php?rid=4411897&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21244367%26dopt%3DAbstract Authors: He X, Zheng YM, Qiu S, Qi YP, Zhang Y The aims of this study were to determine whether stem cells could be isolated from amniotic fluid of goat fetus at terminal gestational age, and to determine if these stem cells could differentiate into adipogenic cells, and be transfected with a reporter gene, enhanced green fluorescence protein (EGFP). The stem cells were isolated from amniotic fluid of goat fetus at terminal gestational age, induced to differentiate into adipogenic cells in vitro, and transfected with EGFP gene using lipofection. Markers associated with undifferentiated amniotic fluid-derived stem (AFS) cells were tested by RT-PCR. The results demonstrated that AFS cells could be isolated from amniotic fluid of goat fetus at terminal gestational age, and could different... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411897 Wed, 19 Jan 2011 00:00:00 +0100 4411897 http://www.medworm.com/index.php?rid=4411901&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21241248%26dopt%3DAbstract Authors: Verghese E, Zhuang J, Saiti D, Ricardo SD, Deane JA Primary cilia are non-motile sensory organelles that project from cells in many tissues. The role of renal primary cilium-based signalling in regulating epithelial cell proliferation and differentiation is highlighted by studies showing that defects of the cilium lead to epithelial de-differentiation, over proliferation and polycystic kidney disease. Recent studies show that renal primary cilia may also play a role in controlling epithelial differentiation during renal repair. After injury, renal cilium length increases dramatically and then undergoes a normalisation that coincides with structural and functional repair in both human patients and mouse models of renal injury. These changes in cilium length are likely to modula... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411901 Mon, 17 Jan 2011 00:00:00 +0100 4411901 http://www.medworm.com/index.php?rid=4411900&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21241249%26dopt%3DAbstract Authors: Meng L, Feng B, Tao H, Yang T, Meng Y, Zhu W, Huang C (3R,6R)-Bassiatin (1) was isolated from the endogenous fungus Fusarium oxysporum J8-1-2. The purpose of this study was to investigate the antiestrogen activity of (3R,6R)-Bassiatin (1) in MCF-7 human breast cancer cells. We found that estrogen-dependent cell growth was inhibited by (3R,6R)-bassiatin in MCF-7 estrogen receptor α (ERα)-positive breast cancer cells. In addition, the mRNA and protein levels of ERα and estrogen-responsive gene cyclinD1 were down-regulated by (3R,6R)-bassiatin in the presence of 17β-estradiol. Co-treatment of 17β-estradiol and (3R,6R)-bassiatin increased phospho-CyclinD1 (Thr286), an indicator of CyclinD1 phosphorylation-dependent degradation. However, this effect was not obvious in the abse... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411900 Mon, 17 Jan 2011 00:00:00 +0100 4411900 http://www.medworm.com/index.php?rid=4411899&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21241250%26dopt%3DAbstract Conclusion. These results demonstrated that MM cells resistant to anticancer agents respond radiation in different levels. These findings may be important in the clinical applications of radiation therapy in the treatment of vincristine resistant MM. PMID: 21241250 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411899 Mon, 17 Jan 2011 00:00:00 +0100 4411899 http://www.medworm.com/index.php?rid=4411904&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21231917%26dopt%3DAbstract Authors: Kaida A, Sawai N, Sakaguchi K, Miura M The fluorescent ubiquitination-based cell cycle indicator (Fucci) is able to visualize dynamics of cell cycle progression in live cells; G1 and S/G2/M phase cells expressing Fucci emit red and green fluorescence, respectively. This system could be applied to cell kinetic analysis of tumor cells in the field of cancer therapy; however, it is still unclear how fluorescence kinetics change after various treatments, including exposure to anti-cancer agents. To explore this, we arrested live HeLa cells expressing the Fucci probes at various cell cycle stages and observed the fluorescence, in conjunction with flow cytometric analysis. X-irradiation, hydroxyurea (HU), and nocodazole arrest cells at G2/M boundary, early S-phase, and early M-phase... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411904 Fri, 14 Jan 2011 00:00:00 +0100 4411904 http://www.medworm.com/index.php?rid=4411903&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21231918%26dopt%3DAbstract Authors: Asai T, Hayashi T, Kuroki K, Okano M, Kiriyama T, Kawai T To develop a bone substitute with shape-generating properties, we focused our attention on dextrin, which has a low viscosity. After considering methods of evaluation for research and development, we started by using cells that are widely used for safe biological evaluations in the field of dentistry and conducted in vitro evaluations. In this experiment, we variously added concentrations of 0.1, 1.0, and 10 mmol/l of dextrin to a culture medium in order to examine the effects on L929 mouse fibroblasts in vitro. As a result, L929 promoted the proliferative activity during the culture period as the concentration of added dextrin became lower, and in particular, the 0.1 and 1-mmol/l addition group showed higher values tha... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411903 Fri, 14 Jan 2011 00:00:00 +0100 4411903 http://www.medworm.com/index.php?rid=4411902&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21235529%26dopt%3DAbstract In conclusion, MSCs in HPL-supplemented media maintained similar growing potential and phenotype, while osteogenic potential was enhanced. HPL offers a promising alternative to FCS for MSC expansion for clinical application, especially in bone injury diseases. PMID: 21235529 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411902 Fri, 14 Jan 2011 00:00:00 +0100 4411902 http://www.medworm.com/index.php?rid=4411905&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21231912%26dopt%3DAbstract Authors: Sugiura T Tripartite motif (TRIM) family proteins, comprising ring finger, B-box and coiled-coil domains, are involved in various cellular processes including tumor development and antiviral response. One of the family proteins, TRIM31, was originally identified as a gene induced by growth-suppressive retinoid. Our previous study showed that TRIM31 is upregulated in stomach cancer and that TRIM31 protein possesses the common features of the TRIM protein family, for example, ubiquitin ligase activity and homo-ologomerization tendency. Interestingly, TRIM31 negatively regulates growth of certain cell types despite its overexpression in gastric cancer tissues. We herein demonstrated that upon exogenous expression in 293 cells, TRIM31 is polyubiquitylated, which promotes its degra... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4411905 Thu, 13 Jan 2011 00:00:00 +0100 4411905 http://www.medworm.com/index.php?rid=4346874&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21166654%26dopt%3DAbstract Authors: Eda H, Burnette BL, Shimada H, Hope HR, Monahan JB The aim of this study is to investigate whether phosphatidylinositol-3-kinase (PI3K) is involved in interleukin-1β (IL-1β)-induced IL-6 production in A549 (human lung adenocarcinoma epithelial cell) and human rheumatoid arthritis synovial fibroblast (RASF). PI3K inhibitor, LY294002 significantly reduced IL-1β-induced IL-6 production in A549 cells but not in RASF, indicating that IL-1β-induced IL-6 production was partially mediated by PI3K in A549 cells but not in RASF. Small interfering RNA (siRNA) of IL-1 receptor-associated kinase 4 (IRAK4) treatment decreased IRAK4 mRNA level by up to 90% in A549 cells. In this condition, IL-1β-induced increase of IL-6 mRNA and protein level was decreased by up to 93% and 70%, respecti... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4346874 Mon, 20 Dec 2010 00:00:00 +0100 4346874 http://www.medworm.com/index.php?rid=4346875&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21166652%26dopt%3DAbstract Authors: He FF, Zhang C, Chen S, Deng BQ, Wang H, Shao N, Tian XJ, Fang Z, Sun XF, Liu JS, Zhu ZH, Meng XF Proteinuria is a well-established exacerbating factor of chronic kidney diseases. However, the injurious effects of protein overload on podocytes and the underlying mechanisms are still poorly understood. In the present study, we examined the effects of high concentrations of albumin on podocytes and investigated the role of CD2-associated protein (CD2AP) in albumin overload-induced podocyte apoptosis. Conditionally immortalized mouse podocytes were cultured in vitro and treated with different concentrations of bovine serum albumin (BSA). In addition, CD2AP eukaryotic expression vector or siRNA was transfected into podocytes before they were exposed to BSA. Podocyte apoptosis, exp... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4346875 Fri, 17 Dec 2010 00:00:00 +0100 4346875 http://www.medworm.com/index.php?rid=4346878&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21155712%26dopt%3DAbstract Authors: Karpukhin AV, Avkhacheva NV, Kulakova II, Yakovlev RY, Yashin VA, Lisichkin GV, Safronova VG Detonation nanodiamond (ND) holds much promise for biological studies and medical applications. Properties like size of particles, inclination for modification of their surface and unambiguous biocompatibility are crucial. Of prime importance is interaction between ND and immune cells which supervise foreign intrusion into an organism and eliminate it. Neutrophils are more reactive in inflammatory response implementing cytotoxical arsenal including reactive oxygen species (ROS). Aim of the work was to estimate aptitude of two ND samples (produced by Diamond Center, Russia, and PlasmaChem, Germany) to keep vitality of neutrophils from inflammatory site. The ability of cells to generate ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4346878 Wed, 15 Dec 2010 00:00:00 +0100 4346878 http://www.medworm.com/index.php?rid=4346877&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21155713%26dopt%3DAbstract Conclusion: PMMA-sorbitol capsules have been developed and tested which make them promising devices for local antibiotic delivery. PMID: 21155713 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4346877 Wed, 15 Dec 2010 00:00:00 +0100 4346877 http://www.medworm.com/index.php?rid=4346876&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21155714%26dopt%3DAbstract Authors: Polupanov AS, Nazarko VY, Sibirny AA Orthologs of Saccharomyces cerevisiae Ccz1, Mon1 and Ypt7 genes in the methylotrophic yeast, Pichia pastoris, have been identified. These genes encode proteins which act as a complex, being involved in degradation of oleate-induced peroxisomes, Cvt-pathway and non-specific macroautophagy in S. cerevisiae. Ccz1, Mon1 and Ypt7 gene orthologs are essential for multiple delivery pathways in P. pastoris. Strains with deletion of either of these genes displayed complete deficiency in pexophagy, non-specific macroautophagy and the biosynthetic Cvt pathway. The data suggest that Ccz1, Mon1 and Ypt7 genes are involved in degradation of both small oleate-induced and large methanol-induced peroxisomes. The data suggest conservative functions of Ccz1, ... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4346876 Wed, 15 Dec 2010 00:00:00 +0100 4346876 http://www.medworm.com/index.php?rid=4346884&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21143203%26dopt%3DAbstract In conclusion, 5-aza inhibited benzene-induced apoptosis through down-regulating caspase-3 and bax and up-regulating bcl-2 mRNA expression, whereas effect of TSA on apoptosis dominatingly affected bcl-2 mRNA expression, and 5-aza together with TSA had no synergic effect on benzene-induced apoptosis. PMID: 21143203 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4346884 Tue, 14 Dec 2010 00:00:00 +0100 4346884 http://www.medworm.com/index.php?rid=4346883&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21143204%26dopt%3DAbstract Authors: Mallick SK, Maiti S, Bhutia SK, Maiti TK Mushroom derived polysaccharides like β-Glucan are being investigated for therapeutic properties for a long time but their mode of action of immunomodulatory properties are not well established. In the present study a heteroglucan from Astraeus hygrometricus designated as AE2 is investigated for its macrophage stimulatory properties using RAW 264.7 cell line. An augmentation of Nitric Oxide production is observed in presence of AE2 in a dose dependent manner due to up regulation of iNOS expression hence NF κB pathway is investigated. RAW 264.7 cells endured phosphorylation of Ikk and the subsequently NF κB translocated to the nucleus. Further the PKC level of the cells enhanced significantly. We also found that AE2 could induce the p... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4346883 Tue, 14 Dec 2010 00:00:00 +0100 4346883 http://www.medworm.com/index.php?rid=4346882&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21143205%26dopt%3DAbstract In this study, we investigate the functional importance of low E-cadherin expression in leukemia cells behaviours and investigate its effects in the abnormal interaction of leukemic cells with stromal cells. After expression of E-cadherin was restored by a demethylating agent in leukemia cells, E-cadherin specific adhesion was enhanced. Additionally, siRNA mediated silencing of E-cadherin in Raji cells resulted in a reduction of cell homophilic adhesion and enhancement of cell proliferation and colony formation. These results suggest that low-expression of E-cadherin contributes to the vigorous growth and transforming ability of leukemic cells. PMID: 21143205 [PubMed - as supplied by publisher] (Source: Cell Biology International) Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4346882 Tue, 14 Dec 2010 00:00:00 +0100 4346882 http://www.medworm.com/index.php?rid=4346881&cid=s_35393_171_f&fid=35393&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21143206%26dopt%3DAbstract Authors: Zhao L, Cao F, Yin T, Sun D, Cheng K, Zhang J, Wang H Abstract Endothelial progenitor cells (EPCs) regenerate the vascular endothelial cells and keep the integrity of the vascular endothelium, and thus may retard the onset of atherosclerosis. Steady state levels of EPCs in the circulation were found to be correlated with cardiovascular event risks. Given the close relationship between insulin and the cardiovascular system, we tested the long term effects of moderate dose insulin treatment on bone marrow derived EPCs. Rat bone marrow EPCs were exposed to various levels of insulin under normal (5 mmol/L) or high (40 mmol/L) glucose conditions for 7 days. Insulin at levels near the physiological range (0.1, 1 nmol/L) up-regulated EPCs proliferation, stimulated nitric oxide (NO) p... Cell Biology International journals http://www.medworm.com/rss/comments.php?id=4346881 Tue, 14 Dec 2010 00:00:00 +0100 4346881