MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Cell Proliferation' source. Tue, 07 Feb 2012 08:48:53 +0100 http://www.medworm.com/index.php?rid=5665139&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2012.00808.x ConclusionOur study showed that bFGF supplementation of UC‐MSC culture enhanced the cells' growth kinetics without compromising their nature. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5665139 Wed, 01 Feb 2012 05:00:00 +0100 5665139 http://www.medworm.com/index.php?rid=5645120&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2012.00807.x Conclusions:  SphK1 and SphK2 are involved in proliferation of glioma cells in hypoxic conditions through distinct signalling pathways. SphK1, but not SphK2, promotes cell population expansion in hypoxic conditions by activating ERK. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5645120 Wed, 01 Feb 2012 05:00:00 +0100 5645120 http://www.medworm.com/index.php?rid=5645122&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2012.00806.x Conclusion:  Our results suggest that canonical Wnt/β‐catenin signalling promotes proliferation and odonto/osteogenic differentiation of SCAP. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5645122 Mon, 30 Jan 2012 05:00:00 +0100 5645122 http://www.medworm.com/index.php?rid=5645121&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00805.x Conclusion:  Polyploid H1 (ES) cells retained pluripotency in vitro, without LIF with nanog over‐expression. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5645121 Mon, 30 Jan 2012 05:00:00 +0100 5645121 http://www.medworm.com/index.php?rid=5618252&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00803.x Conclusions:  Results revealed that in only 5% DMSO, hSKPs could be cryopreserved for long‐term storage with considerable survival and proliferation levels, without losing multipotency. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5618252 Sun, 22 Jan 2012 14:33:52 +0100 5618252 http://www.medworm.com/index.php?rid=5618253&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00804.x Discussion:  These results indicate that the cells maintained their proliferation rate, and continued expressing mesenchymal stem‐cell markers and pericyte marker 3G5 at late passages. These results also show that the cells were capable of adipogenic differentiation and thus could be a promising source for regeneration and replacement of adipose tissue in reconstructive surgery. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5618253 Fri, 20 Jan 2012 05:00:00 +0100 5618253 http://www.medworm.com/index.php?rid=5579192&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00802.x In this study, rat adipose stem cells (ASCs) were isolated and cultured in adipogenic or normal medium. Their exposure to cyclic mechanical stretch (2000 με, 1 Hz) in the presence of adipogenic medium decreased mRNA and protein level of PPAR‐γ, and increased Runx2 mRNA and protein levels as well as Pref‐1 mRNA level, compared to static samples. ASCs cultured in normal medium without adipogenic induction did not show any significant change in mRNA expression of PPAR‐γ, Runx2, nor Pref‐1 irrespective of mechanical loading. Stretching induced phosphorylation of extracellular signal‐regulated protein kinases 1 and 2 (ERK1/2) during the induction period. It was concluded that mechanical stretch inhibited adipogenesis and stimulated osteogenesis of these ASCs in the presence of... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5579192 Thu, 12 Jan 2012 12:41:44 +0100 5579192 http://www.medworm.com/index.php?rid=5570496&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00801.x Conclusion:  Here for the first time, we have demonstrated that either solubilized triterpene acids or lectins and combinations thereof, induce dose‐dependent apoptosis in the ALL cell line NALM‐6 via caspase‐8 and ‐9 dependent pathways. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5570496 Sat, 07 Jan 2012 20:57:37 +0100 5570496 http://www.medworm.com/index.php?rid=5513622&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00800.x Conclusions:  Our results suggest that AML could act as a potential anti‐cancer drug. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5513622 Fri, 16 Dec 2011 05:00:00 +0100 5513622 http://www.medworm.com/index.php?rid=5502615&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00799.x Conclusions:  Our results indicate that CV would be an optimal source of MSCs with high expansion potential in a HS propagation system and immunoregulatory capacity of T and B lymphocytes. More than 90% of CV samples achieved large‐scale expansion in HS, which is encouraging for potential clinical applications of these cells. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5502615 Wed, 14 Dec 2011 05:00:00 +0100 5502615 http://www.medworm.com/index.php?rid=5502614&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00796.x Conclusions:  This study demonstrated that coronopilin efficiently inhibited leukaemia cell population growth by triggering cell type‐specific responses. Moreover, coronopilin‐mediated cell population expansion inhibition was specific to neoplastic cells, as normal white blood cell viability was not significantly affected. Thus, coronopilin may represent an interesting new chemical scaffold upon which to develop new anti‐leukaemic agents. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5502614 Wed, 14 Dec 2011 05:00:00 +0100 5502614 http://www.medworm.com/index.php?rid=5502617&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00795.x Conclusions:  Our new skincare formulation obtained from E. purpurea provides important neuroprotective effects of UV irradiation and could be used together with SPFs to prevent chronic deleterious effects of solar exposure. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5502617 Tue, 13 Dec 2011 05:00:00 +0100 5502617 http://www.medworm.com/index.php?rid=5502616&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00798.x Conclusions:  Taken together, IGF2BP1 and its downstream target IGF2 could be a crucial axis for miR‐494 in regulation of the destiny of A549 cells. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5502616 Tue, 13 Dec 2011 05:00:00 +0100 5502616 http://www.medworm.com/index.php?rid=5484789&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00791.x Conclusions:  Plant‐derived natural compounds are an important source for development of cancer‐fighting drugs. This study has demonstrated strong phototoxic activity of the coumarin fraction of C. pungens, a plant which, to our knowledge, has never been studied before. This investigation offers a new perspective for developing other formulations potentially useful in photodynamic therapy for treatment of non‐melanoma skin cancers as well as melanomas. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5484789 Wed, 07 Dec 2011 05:00:00 +0100 5484789 http://www.medworm.com/index.php?rid=5484788&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00792.x Conclusion:  Overexpression of Id3 triggered apoptosis in A549 human lung adenocarcinoma cells, implicating Id3 in negative control of tumour growth. These Id3‐induced pro‐apoptotic signalling pathways require further study, but this preliminary investigation suggests that Id3 regulation could be exploited in anti‐tumour therapies. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5484788 Wed, 07 Dec 2011 05:00:00 +0100 5484788 http://www.medworm.com/index.php?rid=5513621&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00797.x Conclusions:  Our findings showed that P. guajava L. extract was able to exert anti‐cancer activity on cultures in vitro and ex vivo, supporting the hypothesis of its anti malignant pro‐apoptotic modulation. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5513621 Thu, 01 Dec 2011 05:00:00 +0100 5513621 http://www.medworm.com/index.php?rid=5502613&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00790.x AbstractObjectives:  Here we present a model aiming to provide an estimate of time from tumour genesis, for grade II gliomas. The model is based on a differential equation describing the diffusion–proliferation process. We have applied our model to situations where tumour diameter was shown to increase linearly with time, with characteristic diametric velocity.Materials and methods:  We have performed numerical simulations to analyse data, on patients with grade II gliomas and to extract information concerning time of tumour biological onset, as well as radiology and distribution of model parameters.Results and conclusions:  We show that the estimate of tumour onset obtained from extrapolation using a constant velocity assumption, always underestimates biological tumour age, and th... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5502613 Thu, 01 Dec 2011 05:00:00 +0100 5502613 http://www.medworm.com/index.php?rid=5484787&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00793.x AbstractThe aim of our in vitro experiments was to examine the role of transcription factor p53 and the metabolic hormone leptin, in controlling basic functions (proliferation, apoptosis and secretory activity) of ovarian cells, as well as involvement of p53 in mediating or modulating actions of leptin, on ovarian cells. Porcine ovarian granulosa cells, transfected and non‐transfected with a gene construct encoding p53, were cultured with leptin (at concentrations of 0, 1, 10 or 100 ng/ml). Accumulation of p53 and of apoptosis‐related (bax) and proliferation‐related (PCNA, cyclin B1) substances was evaluated by SDS–PAGE‐western blotting. Secretion of progesterone (P4) was measured by RIA. Transfection with the p53 gene construct promoted accumulation of this transcription facto... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5484787 Thu, 01 Dec 2011 05:00:00 +0100 5484787 http://www.medworm.com/index.php?rid=5332855&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00783.x Conclusion:  Resveratrol potentiates cytotoxic properties of both cancer drugs used in the study through increasing their intracellular level due to p‐glycoprotein inhibition and downregulation of mdr1 gene. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5332855 Thu, 20 Oct 2011 04:00:00 +0100 5332855 http://www.medworm.com/index.php?rid=5312251&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00786.x Conclusion:  Collectively, these results indicate that DHK regenerates epidermal tissue mainly through EGFR phosphorylation. As DHK has diverse advantages over recombinant growth factors for commercialization (that is long‐term stability and skin permeability), DHK might be applied to wound‐healing agents and to a basic materials used in cosmetics. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5312251 Wed, 12 Oct 2011 04:00:00 +0100 5312251 http://www.medworm.com/index.php?rid=5312250&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00787.x Conclusion:  MIF plays a pivotal role in proliferation and tumourigenesis of human HeLa cervical carcinoma cells, and may represent a promising therapeutic target for cancer intervention. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5312250 Wed, 12 Oct 2011 04:00:00 +0100 5312250 http://www.medworm.com/index.php?rid=5312252&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00781.x AbstractObjectives:  Utility of hepatic stem cells could provide a novel solution to the severe shortage of human donor livers, for treatment of liver‐related diseases, due to their ability to proliferate and differentiate into functional hepatocytes. Porcine liver tissues also offer an alternative source from human donor livers. However, morphology, phenotype, successful isolation and culture of porcine hepatic stem cells still require much investigation.Materials and methods:  In the present study, we performed partial hepatectomy to activate hepatic oval cells and developed a procedure utilizing enzymatic digestion and density gradient centrifugation to isolate and purify oval cells derived from porcine livers. We identified ovoid cells by their morphological characteristics and p... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5312252 Tue, 11 Oct 2011 04:00:00 +0100 5312252 http://www.medworm.com/index.php?rid=5282880&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00779.x Conclusions: Ex vivo expansion could lead to occurrence of chromosomal abnormality, although here it did not produce excessive proliferative advantage of the expended cells. Importantly, chromosomal alteration seemed not to be inheritable and unlikely to result in malignant transformation. However, further in‐depth evaluation of potential clinical risks of chromosomal abnormality is warranted. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5282880 Tue, 04 Oct 2011 04:00:00 +0100 5282880 http://www.medworm.com/index.php?rid=5282879&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00784.x AbstractProstate cancer is a serious molecular disorder that arises because of reduction in tumour suppressors and overexpression of oncogenes. The malignant cells survive within the context of a three‐dimensional microenvironment in which they are exposed to mechanical and physical cues. These signals are, nonetheless, deregulated through perturbations to mechanotransduction, from the nanoscale level to the tissue level. Increasingly sophisticated interpretations have uncovered significant contributions of signal transduction cascades in governing prostate cancer progression. To dismantle the major determinants that lie beneath disruption of spatiotemporal patterns of activity, crosstalk between various signalling cascades and their opposing and promoting effects on TRAIL‐mediated act... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5282879 Tue, 04 Oct 2011 04:00:00 +0100 5282879 http://www.medworm.com/index.php?rid=5282878&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00780.x AbstractNotch signalling is a highly conserved intercellular signal transfer mechanism that includes canonical and non‐canonical pathways. It regulates differentiation and proliferation of stem/progenitor cells by means of para‐inducing effects. Expression and activation of Notch signalling factors (receptors and ligands) are critical not only for development of the dental germ but also for regeneration of injured tissue associated with mature teeth. Notch signalling plays key roles in differentiation of odontoblasts and osteoblasts, calcification of tooth hard tissue, formation of cusp patterns and generation of tooth roots. After tooth eruption, Notch signalling can also be triggered in dental stem cells of the pulp, where it induces them to differentiate into odontoblasts, thus gene... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5282878 Tue, 04 Oct 2011 04:00:00 +0100 5282878 http://www.medworm.com/index.php?rid=5332854&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00788.x Conclusions:  We demonstrate for the first time that chitosan enhanced mineralization by upregulating the associated genes. Thus, the study may help clinical situations promoting use of chitosan in bone mineralization, necessary for healing non‐union fractures and more. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5332854 Sat, 01 Oct 2011 04:00:00 +0100 5332854 http://www.medworm.com/index.php?rid=5312249&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00789.x Conclusions:  These results demonstrate that the novel 17β‐oestradiol derivative C9, in combination with DCA is a potent anti‐proliferation treatment, with properties of selectivity towards tumourigenic cells. Thus, this warrants further studies as a potential combination chemotherapeutic agent for further cancer cell lines. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5312249 Sat, 01 Oct 2011 04:00:00 +0100 5312249 http://www.medworm.com/index.php?rid=5282877&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00785.x AbstractObjectives:  Aberrant Met signalling is associated with aggressive cancer cell phenotypes. γ‐tocotrienol displays potent anti‐cancer activity that is associated with suppression of HER/ErbB receptor signalling. Experiments were conducted to investigate the effects of γ‐tocotrienol treatment on HGF‐dependent +SA mammary tumour cell proliferation, upon Met activation.Materials and methods:  The +SA cells were maintained in serum‐free defined media containing 10 ng/ml HGF as the mitogen. Cell viability was determined using the MTT assay, western blot analysis was used to measure protein expression, and Met expression and activation were determined using immunofluorescent staining.Results and conclusions:  Treatment with γ‐tocotrienol or Met inhibitor, SU11274, s... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5282877 Sat, 01 Oct 2011 04:00:00 +0100 5282877 http://www.medworm.com/index.php?rid=5247766&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00778.x Conclusion:  Stem cells appear to be more tolerant to heat stress than non‐stem cells. Incubation of a heterogeneous cell population in heat‐stress conditions resulted in increased stem cell numbers. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247766 Sat, 24 Sep 2011 02:02:26 +0100 5247766 http://www.medworm.com/index.php?rid=5247761&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00774.x Conclusions:  The results reported suggest that gastric cells can be useful as screening population for appropriate study of developmental toxicity. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247761 Sat, 24 Sep 2011 02:02:25 +0100 5247761 http://www.medworm.com/index.php?rid=5247760&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00773.x Conclusion:  Use of Cell Dissociation Solution, combined with slow adaptation protocol, allows a fast switch from the mechanical passaging technique to a single‐cell split technique, generating stable and robust hESC cell lines, which allow for large scale expansion of hESC for TE purposes. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247760 Sat, 24 Sep 2011 02:02:23 +0100 5247760 http://www.medworm.com/index.php?rid=5247757&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00772.x Conclusion:  Our results strongly suggest the involvement of the extrinsic pathway of apoptosis in cells treated by both the original EA extract and its major component, I3‐O‐R. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247757 Sat, 24 Sep 2011 02:02:21 +0100 5247757 http://www.medworm.com/index.php?rid=5247755&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00771.x Conclusions:  Taken together, our results suggest that OSC/LSS expression and activity are required to maintain cell self‐renewal and may be involved in the self‐renewal versus differentiation/apoptosis decision making, by keeping cells in a self‐renewal state. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247755 Sat, 24 Sep 2011 02:02:19 +0100 5247755 http://www.medworm.com/index.php?rid=5247754&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00770.x This study has aimed to repopulate ‘primitive’ cells from late‐passage mesenchymal stem cells (MSCs) of poor multipotentiality and low cell proliferation rate, by simply altering plating density.Materials and methods:  Effects of low density culture compared t high density culture on late‐passage bone marrow (BM)‐derived MSCs and pluripotency markers of multipotentiality were investigated. Cell proliferation, gene expression, RNA interference and differentiation potential were assayed.Results and conclusions:  We repopulated ‘primitive’ cells by replating late‐passage MSCs at low density (17 cells/cm2) regardless of donor age. Repopulated MSCs from low‐density culture were smaller cells with spindle shaped morphology compared to MSCs from high‐density culture. The... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247754 Sat, 24 Sep 2011 02:02:17 +0100 5247754 http://www.medworm.com/index.php?rid=5247753&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00769.x Conclusions:  The binding of TNF‐α to its receptors results in the activation of multiple signaling pathways, which actively interact with each other to regulate the differentiation, proliferation, survival and apoptosis of MSCs. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247753 Sat, 24 Sep 2011 02:02:15 +0100 5247753 http://www.medworm.com/index.php?rid=5247752&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00768.x Conclusions:  Results show that although osteoclastic precursors reside in the CD14+ cell subpopulation, other populations (such as CD14− cells) derived from PBMC, have the ability to modulate osteoclastogenesis positively. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247752 Sat, 24 Sep 2011 02:02:14 +0100 5247752 http://www.medworm.com/index.php?rid=5247748&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00766.x AbstractObjectives:  Cancer stem cells make up a subpopulation of cells within tumours that drive tumour initiation, growth and recurrence. They are resistant to many current types of cancer treatment, causing failure of such therapeutic approaches, including chemotherapy and radiotherapy. In the study described here, anti‐proliferative effects of 3‐O‐methylfunicone (OMF), a metabolite from Penicillium pinophilum, were investigated on human breast cancer MCF‐7 cells and cancer stem cells selected as mammospheres derived from MCF‐7s.Materials and methods:  Stemness markers were analysed on isolated mammospheres showing positive expression of CD24, CD29, CD44, CD133, CD184 and CD338. Cell proliferation and apoptosis were analysed by flow cytometry and RT‐PCR. Cell colony form... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247748 Sat, 24 Sep 2011 02:02:12 +0100 5247748 http://www.medworm.com/index.php?rid=5247747&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00767.x AbstractAccurate chromosome segregation relies on activity of the spindle assembly checkpoint, a surveillance mechanism that prevents premature anaphase onset until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate. Defects in this mechanism contribute to chromosome instability and aneuploidy, a hallmark of malignant cells. Here, we review the molecular mechanisms of activation and silencing of the spindle assembly checkpoint and its relationship to tumourigenesis. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=5247747 Sat, 24 Sep 2011 02:02:10 +0100 5247747 http://www.medworm.com/index.php?rid=4975526&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00762.x Conclusions:  These results indicate that EMPs may inhibit osteoblastic differentiation and attachment to prevent ankylosis and allow other cell types to regenerate periodontal tissues. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4975526 Tue, 28 Jun 2011 22:44:43 +0100 4975526 http://www.medworm.com/index.php?rid=4975525&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00764.x AbstractThe aim of this investigation was to determine whether tumour necrosis factor‐alpha (TNF‐α) has any effect on endothelial progenitor cells (EPCs). Total mononuclear cells were isolated from peripheral blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin‐coated culture dishes. After 7 days culture, attached cells were stimulated with tumour necrosis factor‐α (final concentrations: 0, 10, 20, 50 and 100 mg/l) for 0, 6, 12, 24 and 48 h. EPCs were characterized as adherent cells double positive for DiLDL‐uptake and lectin binding, by direct fluorescence staining. EPC proliferation and migration were assayed using the MTT assay and modified Boyden chamber assay, respectively. EPC adhesion assay was performed by re‐plating tho... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4975525 Tue, 28 Jun 2011 22:44:42 +0100 4975525 http://www.medworm.com/index.php?rid=4975524&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00763.x AbstractEvidence for a functional role for extracellular matrix (ECM) proteins in adipose tissue is demonstrated in dynamic changes in expression of ECM genes during adipocyte differentiation and in obesity. Components of the ECM may regulate adipose cell number expansion by restricting pre‐adipocyte proliferation, regulating apoptosis and inhibiting adipogenesis. Although pre‐adipocytes express multiple proteoglycans, their role in pre‐adipocyte proliferation up to now has remained unknown. The study described here was conducted to characterize roles of small leucine‐rich proteoglycans (SLRPs) in adipocyte proliferation. Pre‐adipocytes were seeded on plates coated with biglycan and decorin and were allowed to differentiate. In addition, pre‐adipocytes were incubated on plates ... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4975524 Tue, 28 Jun 2011 22:44:41 +0100 4975524 http://www.medworm.com/index.php?rid=4975523&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00765.x Conclusion:  These findings suggest that Dsg3 depletion by RNAi reduces cell proliferation, which is likely to be secondary to a defect in cell–cell adhesion, an essential function required for cell differentiation and morphogenesis. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4975523 Tue, 28 Jun 2011 22:44:39 +0100 4975523 http://www.medworm.com/index.php?rid=4902051&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00757.x Conclusions:  Our results show CXCL13‐mediated PCa cell invasion requires Akt and ERK1/2 activation and suggests a new role for DOCK2 in proliferation of hormone‐refractory CXCR5‐positive PCa cells. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4902051 Sun, 05 Jun 2011 23:00:00 +0100 4902051 http://www.medworm.com/index.php?rid=4902050&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00759.x Conclusions:  Here, cells from UCM represented an intermediate stage between pluripotent embryonic and adult stem cells. Size‐sieving can be used to isolate more rapidly proliferating cell populations. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4902050 Sun, 05 Jun 2011 23:00:00 +0100 4902050 http://www.medworm.com/index.php?rid=4902049&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00760.x Conclusions:  Our results demonstrate therapeutic potential of 4HBZ and are consistent with a mechanism involving suppression of Rel A nuclear translocation and inhibition of COX‐2 transcription. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4902049 Sun, 05 Jun 2011 23:00:00 +0100 4902049 http://www.medworm.com/index.php?rid=4902048&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00761.x Conclusions:  Our results suggest that inhibition of sphingolipid synthesis by myriocin in melanoma cells may inhibit expression of cdc25C or activate expression of p53 and p21waf1/cip1, followed by inhibition of cyclin B1 and cdc2, resulting in G2/M arrest of the cell cycle and cell population growth inhibition. Thus, modulation of sphingolipid metabolism by myriocin may be a potential target of mechanism‐based therapy for this type of skin cancer. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4902048 Sun, 05 Jun 2011 23:00:00 +0100 4902048 http://www.medworm.com/index.php?rid=4902047&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00758.x AbstractObjective:  Alterations in plasma lipid profile and in intracellular cholesterol homoeostasis have been described in various malignancies; however, significance of these alterations, if any, in cancer biology is not clear. The aim of the present study was to investigate a possible correlation between alterations in cholesterol metabolism and expansion of leukaemia cell numbers.Materials and methods:  Lipid profiles in plasma and in primary leukaemia cells isolated from patients with acute or chronic lymphocytic leukaemia (ALL and CLL) were studied.Results and conclusions:  Decreased levels of HDL‐C were observed in plasma of leukaemic patients, levels of total cholesterol, LDL‐C, triglycerides and phospholipids were unchanged or only slightly increased. As compared to nor... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4902047 Tue, 31 May 2011 23:00:00 +0100 4902047 http://www.medworm.com/index.php?rid=4758554&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00752.x AbstractThe relationship of cyclin‐dependent kinase inhibitor 3 (CDKN3) with tumours has previously been presented in a number of publications. However, the molecular network and interpretation of CDKN3 through the cell cycle between non‐malignancy associated hepatitis/cirrhosis and hepatocellular carcinoma (HCC) have remained to be elucidated. Here, we have constructed and analysed significant high expression gene CDKN3 activated and inhibited cell cycle networks from 25 HCC versus 25 non‐malignancy associated hepatitis/cirrhosis patients (viral infection HCV or HBV) in GEO Dataset GSE10140‐10141, by combination of a gene regulatory network inference method based on linear programming, and decomposition procedure using CapitalBio MAS 3.0 software, based on integration of public da... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758554 Thu, 28 Apr 2011 07:44:43 +0100 4758554 http://www.medworm.com/index.php?rid=4758553&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00749.x Conclusion:  We have demonstrated that K3O‐ir and R3O‐ir induce apoptosis in human lymphoblastoid cells by the extrinsic pathway of apoptosis. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758553 Thu, 28 Apr 2011 07:44:42 +0100 4758553 http://www.medworm.com/index.php?rid=4758552&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00750.x Conclusions:  EMPs promoted osteogenic differentiation of pBMSCs, and the tissue‐engineered complex of EMPs, pBMSCs and CPC scaffold may be a valuable alternative to be used in periodontal bone tissue engineering and regeneration. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758552 Thu, 28 Apr 2011 07:44:41 +0100 4758552 http://www.medworm.com/index.php?rid=4758551&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00751.x Conclusions:  Fibroblasts are able to induce osteoclastogenesis by paracrine mechanisms, and age and anatomical location affect this ability. Other factors produced by fibroblasts, in addition to M‐CSF and RANKL, appear to contribute to observed osteoclastogenic potential. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758551 Thu, 28 Apr 2011 07:44:40 +0100 4758551 http://www.medworm.com/index.php?rid=4758550&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00755.x Conclusions:  From these results, it is suggested that IGF1 might inhibit insulinogenic differentiation of HEAC, whereas IGF2 enhances differentiation, and that enhancement of IGF2 appeared to be mediated via IR. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758550 Thu, 28 Apr 2011 07:44:40 +0100 4758550 http://www.medworm.com/index.php?rid=4758549&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00754.x Conclusions:  This study demonstrates the possibility of culturing human hair follicle stem cells without causing any significant changes to phenotypes of the cells. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758549 Thu, 28 Apr 2011 07:44:39 +0100 4758549 http://www.medworm.com/index.php?rid=4758548&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00747.x Conclusions:  These results indicate that Lenti‐HIF‐1α can induce BMSC overexpression levels of angiogenic and osteogenic genes in vitro in the normoxic state. Further study will be focused on whether HIF‐1α can also improve bone repair in vivo. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758548 Thu, 28 Apr 2011 07:44:38 +0100 4758548 http://www.medworm.com/index.php?rid=4758547&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00748.x Conclusions:  Cell proliferation as indicated by S and SG2M fractions could be estimated specifically for primordial germ and somatic cells. Estimation of total number of germ and somatic cells was not feasible. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758547 Thu, 28 Apr 2011 07:44:37 +0100 4758547 http://www.medworm.com/index.php?rid=4758546&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00746.x Conclusion:  Random loss of cell survival/replication genes was calculated to be low enough for colon stem cells with APC gene mutations to ‘select’ LOH and derivative chromosome combinations favouring tumour cell proliferation. However, cell survival/replication gene loss was calculated to be too high for colonic stem cells lacking MMR genes to survive chromosomal instability, explaining why MMR mutations only produce tumours with diploid chromosome cells. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758546 Thu, 28 Apr 2011 07:44:36 +0100 4758546 http://www.medworm.com/index.php?rid=4758545&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00753.x AbstractCell cycle progression is controlled by both extracellular and intracellular signalling molecules. It has been generally believed that cdc2/CDK1 only control G2‐M transition in mammalian and many other higher eukaryotic cells. Accumulating evidence shows that cdc2 not only promotes G2‐M transition but is also capable of regulating G1 progress and G1‐S transition via association with multiple interphase cyclins; cdc2 activity can be inhibited by p21 and p27, two traditional G1 CDK inhibitors. In addition, cdc2‐cyclin B controls pronuclear union in interphase fertilized eggs. These data suggest that cdc2 may be a pluripotent CDK. Although mechanisms responsible for the multiple functions of cdc2 remain to be further investigated, interactions of cdc2 with pRb and with several... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4758545 Thu, 28 Apr 2011 07:44:35 +0100 4758545 http://www.medworm.com/index.php?rid=4591848&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00739.x AbstractObjectives:  Intercellular cooperation has been hypothesized to enhance cell proliferation during cancer metastasis through autocrine signalling cascades and mathematical models can provide valuable insights into underlying mechanisms of metastatic tumorigenesis. Here, we present a model that incorporates signal‐stimulated cell proliferation, and investigate influences of diffusion‐driven heterogeneity in signal concentration on proliferation dynamics.Materials and methods:  Our model incorporates signal production through both autocrine and paracrine pathways, and signal diffusion and loss for a metastasizing cell population at a host site. We use the signalling pathway of IL‐6 for illustration where this signalling species forms an intermediate complex with its receptor... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591848 Wed, 16 Mar 2011 00:23:07 +0100 4591848 http://www.medworm.com/index.php?rid=4591847&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00737.x Conclusions:  These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591847 Wed, 16 Mar 2011 00:23:06 +0100 4591847 http://www.medworm.com/index.php?rid=4591846&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00733.x Conclusions:  EGFR is part of the mechanism that regulates cell proliferation in rat gastric mucosa during early weaning. We suggest that such responses might depend on activation of MAPK and/or Src signalling pathways and regulation of p21 levels. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591846 Wed, 16 Mar 2011 00:23:06 +0100 4591846 http://www.medworm.com/index.php?rid=4591845&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00738.x AbstractFibrosis is characterized by loss of normal structure and function of a tissue or organ resulting from excessive fibroblast proliferation and extracellular matrix production. Currently, there is no efficient treatment for fibrosis. Herein, we test effects of the drug mithramycin, which targets the Sp1 family of transcription factors, on mRNA expression by human gingival fibroblasts. Mithramycin reduced expression of connective tissue growth factor and type I collagen mRNAs. Microarray profiling revealed that mithramycin selectively blocked expression of cell proliferation and transforming growth factor‐beta (TGF‐β) signalling clusters. These microarray data were validated using real‐time polymerase chain reaction and western blot analyses. Mithramycin suppressed expression o... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591845 Wed, 16 Mar 2011 00:23:05 +0100 4591845 http://www.medworm.com/index.php?rid=4591844&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00743.x Conclusions:  Our data suggest, for the first time, that PIPP may affect development of fertilized mouse eggs by inhibition of level of phosphorylated Akt at Ser473 and subsequent inhibition of downstream signal cascades. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591844 Wed, 16 Mar 2011 00:23:04 +0100 4591844 http://www.medworm.com/index.php?rid=4591843&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00742.x Conclusions:  Phosphorylation at S482 is important for NF90 stability and in regulating its functional role during mitosis. Based on the sequence surrounding S482, mitotic kinase PLK1 is a strong candidate for the enzyme that phosphorylates NF90 at this site. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591843 Wed, 16 Mar 2011 00:23:03 +0100 4591843 http://www.medworm.com/index.php?rid=4591842&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00745.x Conclusion:  Taken together, these results support a possible anti‐cancer and anti‐angiogenetic activity of Zol. Our data may not only provide a basis for the clinical use of this drug as preventive agent of bone metastases but also suggest that Zol deserves attention as an anti‐cancer agent in non‐small‐cell lung cancer. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591842 Wed, 16 Mar 2011 00:23:02 +0100 4591842 http://www.medworm.com/index.php?rid=4591841&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00735.x Conclusions:  Our data collectively suggest that endogenously produced MTA1 constrains osteogenic differentiation of MSCs and that targeting of this molecule may provide a novel strategy for enhancing bone regeneration. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591841 Wed, 16 Mar 2011 00:23:02 +0100 4591841 http://www.medworm.com/index.php?rid=4591840&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00744.x Conclusions:  The data presented suggest that the KCL‐002 hES cell line is pluripotent and harbours a bias in basal differentiation tendencies towards mesodermal and mesenchymal lineage cells. Characterizing innate differentiation propensities of hES cell lines is important for understanding heterogeneity between different cell lines and for further studies aimed at deriving specific lineages from hES cells. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591840 Wed, 16 Mar 2011 00:23:01 +0100 4591840 http://www.medworm.com/index.php?rid=4591839&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2011.00734.x Conclusion:  In the pentaploid–decaploid transition of H1 cells, cell cycle parameters and pluripotency were retained, but morphology and DNA stability were altered. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4591839 Wed, 16 Mar 2011 00:23:00 +0100 4591839 http://www.medworm.com/index.php?rid=4298543&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00741.x (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298543 Thu, 30 Dec 2010 10:48:45 +0100 4298543 http://www.medworm.com/index.php?rid=4298541&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00740.x Conclusions:  These experiments confirmed that MSC cell fate choice depends on minute, cell‐derived forces. Applied force could assist in commercial manufacture of cultured bio‐engineered prostheses for regenerative medicine as it mimics tissue stresses and constitutes a good model for development of tissue substitutes. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298541 Thu, 30 Dec 2010 10:48:43 +0100 4298541 http://www.medworm.com/index.php?rid=4298540&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00736.x AbstractThe use of stem cells derived from adipose tissue as an autologous and self‐replenishing source for a variety of differentiated cell phenotypes, provides a great deal of promise for reconstructive surgery. In this article, we review available literature encompassing methods of extraction of pluripotent adipose stem cells (ASCs) from lipoaspirate locations, their storage, options for culture, growth and differentiation, cryopreservation and its effect on stem cell survival and proliferation, and new technologies involving biomaterials and scaffolds. We will conclude by assessing potential avenues for developing this incredibly promising field. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298540 Thu, 30 Dec 2010 10:48:43 +0100 4298540 http://www.medworm.com/index.php?rid=4298539&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00732.x Conclusions:  Our data suggest that hypoxia is not necessary for maintaining pluripotency of mES cells and appeared to be detrimental during ES differentiation. Moreover, HA may offer a valuable alternative for long‐term culture of mES cells in vitro. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298539 Thu, 30 Dec 2010 10:48:42 +0100 4298539 http://www.medworm.com/index.php?rid=4298538&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00731.x In this study, we have examined growth kinetics, morphological changes, differentiation potential and chromosomal abnormalities, as well as tumour formation potential of rabbit BM‐MSC in long‐term culture.Results and conclusion:  We found that shortly after isolation, proliferation rate of rabbit BM‐MSC decreases until they enter a dormant phase. During this period of quiescence, the cells are large and multinucleate. After some weeks of dormancy we found that several small mononuclear cells originated from each large multinucleate cell. These newly formed cells proliferated rapidly but had inferior differentiation potential. Although they were immortal, they did not have the capability for tumour formation in soft agar assay or in nude mice. This is the first report of spontaneous... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298538 Thu, 30 Dec 2010 10:48:41 +0100 4298538 http://www.medworm.com/index.php?rid=4298537&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00717.x Conclusion:  Microarray data showed that nine miRNAs (miR‐20b*, miR‐28‐5p, miR‐99a, miR‐125b, miR‐151‐3p, miR‐151:9.1, miR‐216a, miR‐223* and miR‐1296) were differentially expressed in most LCLs during long‐term culture. In particular, miR‐125b was up‐regulated in all the tested late‐passage LCLs. miR‐99a, miR‐125b, miR‐216a and miR‐1296 were putative negative regulators of RASGRP3, GPR160, PRKCH and XAF1, respectively, which were found to be differentially expressed in LCLs during long‐term culture in a previous study. Linear regression analysis showed that miR‐200a and miR‐296‐3p correlated with triglyceride and HbA1C levels, respectively, suggesting that miRNA signatures of LCLs could provide information on the donor’s health. In conclus... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298537 Thu, 30 Dec 2010 10:48:40 +0100 4298537 http://www.medworm.com/index.php?rid=4298536&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00716.x Conclusion:  We conclude that TG2 loss in endothelial cells causes reduction in cell number as a result of cell cycle arrest, flaws in adhesion and induction of apoptosis. Our results imply that reduction in cell number and increased apoptosis in response to TG2 silencing is independent of the cell adhesion process. Altogether, our findings underline the significance of TG2 in endothelial cell cycle progression and cell survival, in vitro. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298536 Thu, 30 Dec 2010 10:48:39 +0100 4298536 http://www.medworm.com/index.php?rid=4298534&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00715.x Conclusion:  Fibrin is a suitable matrix for EPC growth, differentiation and angiogenesis capability, suggesting that fibrin gel may be very useful for regenerative medicine. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298534 Thu, 30 Dec 2010 10:48:39 +0100 4298534 http://www.medworm.com/index.php?rid=4298533&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00714.x In this study, we isolated and established an immortalized mesenchymal stem cell (MSC) line derived from foetal porcine pancreas, by transfecting human telomerase reverse transcriptase (hTERT) and called these immortalized pancreatic mesenchymal stem cells (iPMSCs). The iPMSCs have been cultured for more than 80 passages and have capacity to differentiate into neurons, cardiomyocytes, germ cells and islet‐like cells, analysed by morphology, RT‐PCR, western blotting, immunofluorescence, immunocytochemistry and transplantation assay. Islets derived from iPMSCs reversed hyperglycaemia in streptozotocin‐induced diabetic mice and secreted insulin and C‐peptide in vitro. These results demonstrated that iPMSCs might provide unlimited resources for islet replacement therapy and models for ... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298533 Thu, 30 Dec 2010 10:48:38 +0100 4298533 http://www.medworm.com/index.php?rid=4298532&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00713.x Conclusion:  These results suggest that recruitment of the hyper‐activating variant of c‐kit (Kitmu) lead to early recovery of the bone marrow of lethally irradiated mice. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298532 Thu, 30 Dec 2010 10:48:37 +0100 4298532 http://www.medworm.com/index.php?rid=4298531&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00712.x Conclusion:  APC/C‐Cdh1 controls stability of HEC1, ensuring normal cell cycle progression. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4298531 Thu, 30 Dec 2010 10:48:36 +0100 4298531 http://www.medworm.com/index.php?rid=4118206&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00711.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118206 Sat, 30 Oct 2010 03:51:48 +0100 4118206 http://www.medworm.com/index.php?rid=4118205&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00710.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118205 Sat, 30 Oct 2010 03:51:47 +0100 4118205 http://www.medworm.com/index.php?rid=4118204&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00709.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118204 Sat, 30 Oct 2010 03:51:47 +0100 4118204 http://www.medworm.com/index.php?rid=4118203&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00708.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118203 Sat, 30 Oct 2010 03:51:46 +0100 4118203 http://www.medworm.com/index.php?rid=4118202&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00707.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118202 Sat, 30 Oct 2010 03:51:46 +0100 4118202 http://www.medworm.com/index.php?rid=4118201&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00706.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118201 Sat, 30 Oct 2010 03:51:45 +0100 4118201 http://www.medworm.com/index.php?rid=4118200&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00705.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118200 Sat, 30 Oct 2010 03:51:45 +0100 4118200 http://www.medworm.com/index.php?rid=4118199&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00704.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118199 Sat, 30 Oct 2010 03:51:44 +0100 4118199 http://www.medworm.com/index.php?rid=4118198&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00703.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118198 Sat, 30 Oct 2010 03:51:44 +0100 4118198 http://www.medworm.com/index.php?rid=4118197&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00702.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=4118197 Sat, 30 Oct 2010 03:51:43 +0100 4118197 http://www.medworm.com/index.php?rid=3914809&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00701.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3914809 Tue, 31 Aug 2010 05:20:52 +0100 3914809 http://www.medworm.com/index.php?rid=3914808&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00700.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3914808 Tue, 31 Aug 2010 05:20:51 +0100 3914808 http://www.medworm.com/index.php?rid=3914807&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00699.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3914807 Tue, 31 Aug 2010 05:20:51 +0100 3914807 http://www.medworm.com/index.php?rid=3914806&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00698.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3914806 Tue, 31 Aug 2010 05:20:50 +0100 3914806 http://www.medworm.com/index.php?rid=3914805&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00697.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3914805 Tue, 31 Aug 2010 05:20:50 +0100 3914805 http://www.medworm.com/index.php?rid=3914804&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00695.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3914804 Tue, 31 Aug 2010 05:20:49 +0100 3914804 http://www.medworm.com/index.php?rid=3914803&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00694.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3914803 Tue, 31 Aug 2010 05:20:49 +0100 3914803 http://www.medworm.com/index.php?rid=3914802&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00693.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3914802 Tue, 31 Aug 2010 05:20:48 +0100 3914802 http://www.medworm.com/index.php?rid=3914801&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00692.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3914801 Tue, 31 Aug 2010 05:20:48 +0100 3914801 http://www.medworm.com/index.php?rid=3822718&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00696.x Conclusions: Comparison of biological characteristics of AOBs from young adult women with those from elderly women in vitro revealed differences in proliferative capacity and bone formation functions, which decreased with aging. These data indicate that aging may play an important role in pathogenesis of human AOBs loss. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3822718 Wed, 04 Aug 2010 23:00:00 +0100 3822718 http://www.medworm.com/index.php?rid=3885193&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00696.x (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3885193 Sat, 31 Jul 2010 23:00:00 +0100 3885193 http://www.medworm.com/index.php?rid=3844627&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00691.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3844627 Sat, 31 Jul 2010 23:00:00 +0100 3844627 http://www.medworm.com/index.php?rid=3844626&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00690.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3844626 Sat, 31 Jul 2010 23:00:00 +0100 3844626 http://www.medworm.com/index.php?rid=3844625&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00689.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3844625 Sat, 31 Jul 2010 23:00:00 +0100 3844625 http://www.medworm.com/index.php?rid=3844624&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00687.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3844624 Sat, 31 Jul 2010 23:00:00 +0100 3844624 http://www.medworm.com/index.php?rid=3844623&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00686.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3844623 Sat, 31 Jul 2010 23:00:00 +0100 3844623 http://www.medworm.com/index.php?rid=3844622&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00685.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3844622 Sat, 31 Jul 2010 23:00:00 +0100 3844622 http://www.medworm.com/index.php?rid=3844621&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00682.x Abstract (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3844621 Sat, 31 Jul 2010 23:00:00 +0100 3844621 http://www.medworm.com/index.php?rid=3713938&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00682.x Conclusions: MAPK subtypes seemed to regulate chondrogenesis with a delicate balance, interacting with the TGF-[beta]1/Smads signalling pathway. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713938 Thu, 01 Jul 2010 07:56:23 +0100 3713938 http://www.medworm.com/index.php?rid=3713947&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00688.x Objectives: The aim of this study was to determine an optimal culture method for porcine bone marrow-derived endothelial progenitor cells (EPCs).Materials and methods: Mononuclear cells (MNCs) were isolated by density centrifugation and differentiated into EPCs in in vitro. At first-passage, EPCs were cultured at different cell densities (5 × 103, 1 × 104, 2 × 104 or 5 × 104/cm2) and in basic medium (EGM, medium 199, DMEM or 1640) supplemented with FBS (2%, 5%, 10% or 20%) and different combinations of cytokines (VEGF, VEGF + bFGF, VEGF + bFGF + EGF, or VEGF + bFGF + EGF + IGF), the experiment being based on L64 (421) orthogonal design.Results and conclusions: This demonstrated that the optimal culture method for our EPCs displayed higher expansion and migration rates as compared to ot... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713947 Mon, 28 Jun 2010 23:00:00 +0100 3713947 http://www.medworm.com/index.php?rid=3713946&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00691.x Conclusion: These finding may support the idea that in vitro propagation of SSCs could be a useful tool for infertility treatment and study of reproductive biology. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713946 Mon, 28 Jun 2010 23:00:00 +0100 3713946 http://www.medworm.com/index.php?rid=3713945&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00690.x Conclusions: We report for the first time the simultaneous early influence of SRL on phosphorylation status and on protein expression in the total proteome of CCRF-CEM T lymphoblasts and predict that 56% of the proteins interact with each other, highlighting significance of these results. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713945 Mon, 28 Jun 2010 23:00:00 +0100 3713945 http://www.medworm.com/index.php?rid=3713944&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00689.x Conclusion: Our results suggest a novel pathway by which CD44 inactivates Akt, down-regulates EGR-1 expression and inhibits cell proliferation. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713944 Mon, 28 Jun 2010 23:00:00 +0100 3713944 http://www.medworm.com/index.php?rid=3713943&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00687.x Conclusion: Our results showed that LCLs acquired expression phenotype changes involving expression of NF-[kappa]B pathway- and carcinogenesis-related genes during long-term subculture. These differentially expressed genes can be considered to be a gene signature of LCL immortalization or EBV-induced carcinogenesis. Clinical trait-associated expression phenotypes should prove useful in the discovery of new candidate genes for particular traits. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713943 Mon, 28 Jun 2010 23:00:00 +0100 3713943 http://www.medworm.com/index.php?rid=3713942&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00686.x Conclusions: MCCs immortalize spontaneously after extensive cell culture, but still demonstrate stem cell-like qualities. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713942 Mon, 28 Jun 2010 23:00:00 +0100 3713942 http://www.medworm.com/index.php?rid=3713941&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00685.x Conclusion: Our results suggest that p53-dependent G1 arrest in both 1st and 2nd cell cycles may protect human cancer cells from cell death after treatment with IR and Chk1 inhibitors. However, a challenge for future clinical use will be that different cancers display different intrinsic sensitivity to such inhibitors. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713941 Mon, 28 Jun 2010 23:00:00 +0100 3713941 http://www.medworm.com/index.php?rid=3713940&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00684.x Objectives: Curcumin, a natural compound, is a potent anti-cancer agent, which inhibits cell division and/or induces cell death. It is believed that normal cells are less sensitive to curcumin than malignant cells; however, the mechanism(s) responsible for curcumin's effect on normal cells are poorly understood. The aim of this study was to verify the hypothesis that curcumin affects normal cell division by influencing microtubule stability, using mouse oocyte and early embryo model systems.Materials and methods: Maturating mouse oocytes and two-cell embryos were treated with different concentrations of curcumin (10[ndash]50 [mu]m), and meiotic resumption and mitotic cleavage were analysed. Spindle and chromatin structure were visualized using confocal microscopy. In addition, acetylation ... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713940 Mon, 28 Jun 2010 23:00:00 +0100 3713940 http://www.medworm.com/index.php?rid=3713939&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00683.x Conclusions: Biological mechanisms, which can be directly responsible for observed oscillations, are proposed, which relates also to recent work on related topics. Further investigations, both at experimental and at modelling levels, are also suggested. Finally, from a methodological point of view, results obtained represent further confirmation of applicability and usefulness of the PUN approach. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3713939 Mon, 28 Jun 2010 23:00:00 +0100 3713939 http://www.medworm.com/index.php?rid=3515180&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00669.x Conclusion: Karyotypically abnormal chHES-3 cells had a more developed capacity for proliferation, resistance to apoptosis and less genetic stability compared to normal chHES-3 cells and may be an excellent model for studying and characterizing initial stages that determine transition of embryonic stem cells into cancer stem cells. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515180 Thu, 29 Apr 2010 15:53:40 +0100 3515180 http://www.medworm.com/index.php?rid=3515190&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00678.x Conclusion: These findings illustrate how the deleterious effect of T-LGL on neutrophils is balanced, over periods of years, by pulses of compensatory neutrophil production, potentially accounting for the commonly observed prolonged indolent course of the disease. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515190 Tue, 27 Apr 2010 23:00:00 +0100 3515190 http://www.medworm.com/index.php?rid=3515189&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00672.x Objectives: Although there have been major advances in understanding immunopathogenesis of psoriasis, the basic processes causing psoriatic morphology remain to be identified.Materials and methods: Our group has designed a systematic review of studies (1962[ndash]2009) on keratinocyte kinetics in psoriasis. We obtained data from MEDLINE, PubMed, Current Contents, reference lists and specialist textbooks. A general equation for evolution of the differentiated epidermis has been analysed. Necessary conditions for observed qualitative change in homeostasis between normal skin and established psoriatic lesions were determined.Results and discussion: Increase in the number of cell divisions (or imbalance in symmetric division rates of committed progenitor cells) and/or decrease in physiological... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515189 Tue, 27 Apr 2010 23:00:00 +0100 3515189 http://www.medworm.com/index.php?rid=3515188&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00679.x Conclusions: The results of this study amplify previously published data and may contribute to comprehension of erythroid differentiation and identification of new target genes involved in some erythroid concerning diseases. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515188 Tue, 27 Apr 2010 23:00:00 +0100 3515188 http://www.medworm.com/index.php?rid=3515187&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00677.x Conclusion: Data obtained in this study demonstrate the vast potential of this stirred culture system for efficient, reproducible and cost-effective expansion of SCs in vitro. The system has advantages over static culture, which has major obstacles such as lower cell density, is time-consuming and susceptible to contamination. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515187 Tue, 27 Apr 2010 23:00:00 +0100 3515187 http://www.medworm.com/index.php?rid=3515186&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00673.x Conclusions: Our results show that low CDDP doses (0.25[ndash]1 [mu]m) induce cell mortality and cell cycle perturbation, which were more evident when given in combination with PDT, but in contrast to work of other authors no synergistic activity was found. Apoptosis occurred via intrinsic pathways in treated cells, although it did not represent the predominant mode of cell death. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515186 Tue, 27 Apr 2010 23:00:00 +0100 3515186 http://www.medworm.com/index.php?rid=3515185&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00680.x Conclusion: ASC CM-derived TGF-[beta]1-induced chondrogenic differentiation of ASCs resulted in significant reduction in chondrogenic activity after inhibition of the p38 pathway, revealing involvement of this MAPK pathway in TGF-[beta]1 signalling. On the other hand, TGF-[beta]1 signalling also led to SMAD activation that could directly increase chondrogenic activity of ASCs. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515185 Tue, 27 Apr 2010 23:00:00 +0100 3515185 http://www.medworm.com/index.php?rid=3515184&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00674.x Conclusions: We have developed a simple and economical method that effectively isolates HC-free, therapeutically functional mMSCs from bone marrow cell adherent cultures. These cells are suitable for various mechanistic and therapeutic studies in the mouse model. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515184 Tue, 27 Apr 2010 23:00:00 +0100 3515184 http://www.medworm.com/index.php?rid=3515183&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00671.x Accumulating data suggest that cancers contain a fraction of cells called cancer stem cells (CSCs), that may be responsible for upkeep and relapses of disease. In experimental settings, CSCs are regarded as most effective at tumour initiation in in vivo assays. Since the first isolation of cancer stem cells from acute myeloid leukaemia in 1994, cancer stem cells have been identified in human solid tumours and they have also been found in the established cell lines, based on ability of CSCs to form in vitro colonies of a specific morphology, called holoclones. Our study examined the ability of a mouse sarcoma cell line, derived from a lung metastasis of a BALB/c mouse and established as a stably growing line (L1), to produce holoclones in vitro. We aimed to verify a stemness signature of th... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515183 Tue, 27 Apr 2010 23:00:00 +0100 3515183 http://www.medworm.com/index.php?rid=3515182&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00670.x Conclusions: Multipotent DFCs can be induced to differentiate towards osteoblasts, adipocytes or chondrocytes in vitro. Runx2 over-expression up-regulated expression levels of osteoblast/cementoblast-related genes and in vitro enhanced osteogenic differentiation of DFCs. In addition, mutant Runx2-induced changes in DFCs were more prominent than those induced by full-length Runx2. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515182 Tue, 27 Apr 2010 23:00:00 +0100 3515182 http://www.medworm.com/index.php?rid=3515181&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00675.x Conclusions: In Papio ursinus, in marked contrast to rodents and lagomorphs, hTGF-[beta]2 induced large corticalized and vascularized ossicles by day 30 after implantation into the rectus abdominis muscle. This striated muscle contains responding stem cells that enhance the bone induction cascade of hTGF-[beta]2. Induction of bone formation by hTGF-[beta]2 in the non-human primate Papio ursinus may occur as a result of expression of bone morphogenetic proteins on heterotopic implantation of hTGF-[beta]2; the bone induction cascade initiated by mammalian TGF-[beta] proteins in Papio ursinus needs to be re-evaluated for novel molecular therapeutics for induction of bone formation in clinical contexts. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3515181 Tue, 27 Apr 2010 23:00:00 +0100 3515181 http://www.medworm.com/index.php?rid=3471628&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00681.x Conclusions: Renal oncocytomas were composed of two independent compartments: benign oncocytes and pronounced fibrotic stroma, which consisted of proliferating myofibroblasts (SMA- and MIB-1-positive) which were associated with excessive deposition of extracellular matrix (periodic acid Schiff-component, collagen I-, collagen III- and fibronectin-positive, and desmin- and human caldesmon-negative). (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3471628 Wed, 14 Apr 2010 23:00:00 +0100 3471628 http://www.medworm.com/index.php?rid=3471629&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00676.x This study focuses on analysis of in vitro cultures of chondrocytes from ovine articular cartilage. Isolated cells were seeded in Petri dishes, then expanded to confluence and phenotypically characterized by flow cytometry. The sigmoidal temporal profile of total counts was obtained by classic haemocytometry and corresponding cell size distributions were measured electronically using a Coulter Counter. A mathematical model recently proposed (1) was adopted for quantitative interpretation of these experimental data. The model is based on a 1-D (that is, mass-structured), single-staged population balance approach capable of taking into account contact inhibition at confluence. The model's parameters were determined by fitting measured total cell counts and size distributions. Model reliabili... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3471629 Tue, 13 Apr 2010 23:00:00 +0100 3471629 http://www.medworm.com/index.php?rid=3311450&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00659.x Conclusions: ERR[alpha] affects cell cycle mechanisms through modulating mitochondrial mass and function. Dysregulation of this essential pathway leads to elevation in mitochondrial ROS production, which in turn modulates activities of tumour suppressors, resulting in cell cycle arrest. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3311450 Fri, 26 Feb 2010 16:58:07 +0100 3311450 http://www.medworm.com/index.php?rid=3311458&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00668.x Conclusion: Our results demonstrate that rhMK stimulates proliferation of primary articular chondrocytes in vitro and in vivo. The results of this study warrant further examination of rhMK for treatment of animal models of articular cartilage defects. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3311458 Fri, 26 Feb 2010 00:00:00 +0100 3311458 http://www.medworm.com/index.php?rid=3311457&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00660.x The Epstein[ndash]Barr virus (EBV) is utilized as a tool in the study of cellular biology because of its capacity to transform B-lymphocytes. For this reason, EBV is used in conservation of human B-lymphocytes for long periods for subsequent evaluation of lysosomal hydrolase activity. Lymphoblastoid cell lines have several advantages for use over other cell types, such as prompt availability and possibility to develop, characterize and standardize cell banks, to test effects of promising pharmaceutical reagents. The study below presents biochemical data that demonstrate validity of lymphoblastoid cell lines for diagnosis of GM1-gangliosidosis, Gaucher, Fabry and Pompe diseases and mucopolysaccharidosis type I. Cultures were prepared from peripheral blood, collected from 25 normal subjects ... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3311457 Fri, 26 Feb 2010 00:00:00 +0100 3311457 http://www.medworm.com/index.php?rid=3311456&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00666.x Conclusion: The cell line met each of the quality control standards required for the American Type Culture Collection. It had not only preserved the genetic resources of the important Langshan chicken at the cellular level, but also provided valuable material for genomic, post-genomic and somatic cell cloning research and other applications. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3311456 Fri, 26 Feb 2010 00:00:00 +0100 3311456 http://www.medworm.com/index.php?rid=3311455&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00661.x Conclusions: We conclude that blocking Notch signalling with DAPT enhances adipogenesis of differentiated mASCs at an early stage. It may be due to depression of DLK-1/Pref-1 and promotion of de-PPAR-[gamma] activation, which work through inhibition of Notch-2-Hes-1 pathway by DAPT. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3311455 Fri, 26 Feb 2010 00:00:00 +0100 3311455 http://www.medworm.com/index.php?rid=3311454&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00667.x Conclusions: The results, combined with existing data from clonogenic survival assays, support the hypothesis that a dominant effect of radiation in these melanoma lines is the induction of long-term cell cycle arrest. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3311454 Fri, 26 Feb 2010 00:00:00 +0100 3311454 http://www.medworm.com/index.php?rid=3311453&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00665.x Conclusions: Microplate spectrophotometry is a robust and sensitive method to monitor growth of animal cell populations both in the absence and in the presence of cytotoxic drugs. This method implements existing technologies and can be fully automated. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3311453 Fri, 26 Feb 2010 00:00:00 +0100 3311453 http://www.medworm.com/index.php?rid=3311452&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00664.x Conclusions: Raloxifene treatment significantly reduced Ki-67 antigen expression and increased Bcl-2 expression in breast carcinomas of post-menopausal women. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3311452 Fri, 26 Feb 2010 00:00:00 +0100 3311452 http://www.medworm.com/index.php?rid=3311451&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2010.00663.x Conclusion: OMF may have potential as a naturally derived anti-tumour drug for treatment of mesothelioma. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3311451 Fri, 26 Feb 2010 00:00:00 +0100 3311451 http://www.medworm.com/index.php?rid=3171697&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00662.x Conclusions: Altogether, these data suggest a novel function for apigenin, acting as a suppressor of AKT/PKB pathway in PEL cells, and raise the possibility that this agent may have a future therapeutic role in PEL and possibly other malignancies with constitutive activation of the AKT/PKB pathway. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3171697 Thu, 14 Jan 2010 00:00:00 +0100 3171697 http://www.medworm.com/index.php?rid=3124443&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00648.x Conclusions: The data show that exposure to hypoxic conditions (1% O2) of rat and human myoblasts altered their proliferation and differentiation processes. They also show that Epo is not an efficient growth factor to counteract this deleterious effect. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3124443 Mon, 28 Dec 2009 16:15:36 +0100 3124443 http://www.medworm.com/index.php?rid=3124444&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00650.x Conclusions: These results indicate that DNER knockdown in hAMSC accelerated onset of adipogenic differentiation by bypassing mitotic clonal expansion during the early stages of adipogenesis. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3124444 Sun, 27 Dec 2009 00:00:00 +0100 3124444 http://www.medworm.com/index.php?rid=3007905&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00652.x Conclusions: These results suggest that S. typhimurium A1-R monotherapy can effectively treat spinal cord glioma. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3007905 Thu, 19 Nov 2009 00:00:00 +0100 3007905 http://www.medworm.com/index.php?rid=3003622&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00658.x Conclusions: These results demonstrate that the novel phenolic compound 2HBZ is a potent apoptosis-inducing agent in HT-1080 cells and warrants further investigation as a potential chemotherapeutic agent in primary cancer cell models. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3003622 Wed, 18 Nov 2009 00:00:00 +0100 3003622 http://www.medworm.com/index.php?rid=3003625&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00654.x This paper summarises how scaffold proteins affects and regulate the JNK signalling pathway. We believe that some of these scaffold proteins, by virtue of their anchoring and catalytic properties contribute to a high degree of specificity of intra cellular signalling pathways that regulate the progression through the cell cycle. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3003625 Tue, 17 Nov 2009 00:00:00 +0100 3003625 http://www.medworm.com/index.php?rid=3003624&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00657.x Objectives: Previous studies have shown that [gamma]-tocotrienol induces potent anti-proliferative effects on +SA mammary tumour cells in culture; here, investigations have been conducted to determine its effects on intracellular signalling proteins involved in regulating cell cycle progression.Materials and methods: +SA cells were maintained in mitogen-free defined media containing 0 or 4 [mu]m[gamma]-tocotrienol, for 48 h to synchronize cell cycle in G0 phase, and then they were exposed to 100 ng/ml EGF to initiate cell cycle progression. Whole cell lysates were collected at various time points from each treatment group and were prepared for Western blot analysis.Results and conclusions: Treatment with 4 [mu]m[gamma]-tocotrienol significantly inhibited +SA cell proliferation over a 4-day... Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3003624 Tue, 17 Nov 2009 00:00:00 +0100 3003624 http://www.medworm.com/index.php?rid=3003623&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00656.x Conclusions: EMPs promoted cell proliferation and differentiation and gene expression profiles of HBMSCs were affected. This may help elucidation of mechanisms involved in promoting regeneration of periodontal tissues by EMPs. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=3003623 Tue, 17 Nov 2009 00:00:00 +0100 3003623 http://www.medworm.com/index.php?rid=2977107&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00655.x Conclusion: FGF18 seems to play a role in maintenance of chondrocyte properties, although its expression was rather high in dedifferentiated chondrocytes. Upregulation of FGF18 in dedifferentiated chondrocytes implied that it may be a marker of dedifferentiation. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=2977107 Tue, 10 Nov 2009 00:00:00 +0100 2977107 http://www.medworm.com/index.php?rid=2912056&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00649.x Conclusions: This study demonstrates that an extracellular glycosaminoglycan, heparin, finely modulates expression of genes crucial to cell cycle regulation through specific activation of p38 MAP kinase to stimulate colon cancer cell growth. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=2912056 Tue, 20 Oct 2009 23:00:00 +0100 2912056 http://www.medworm.com/index.php?rid=2912055&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00653.x Conclusions: G0 phase cells with resistance to 5-FU damage expressed Oct3/4, Nanog and Sox2. This indicated that these cells were undifferentiated, but had the ability to terminally differentiate into downstream-type cells. They possessed stem cell properties. The results are consistent with Oct3/4, Nanog and Sox2-expressing cells being considered as tracheal stem cells. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=2912055 Tue, 20 Oct 2009 23:00:00 +0100 2912055 http://www.medworm.com/index.php?rid=2890460&cid=s_32058_171_f&fid=32058&url=http%3A%2F%2Fdx.doi.org%2F10.1111%252Fj.1365-2184.2009.00651.x Conclusions: Both full-length and truncated syndecan-1 inhibited proliferation of the mesenchymal tumour cells, providing new insights into the importance for cancer growth of different functional domains of this proteoglycan. (Source: Cell Proliferation) Cell Proliferation journals http://www.medworm.com/rss/comments.php?id=2890460 Tue, 13 Oct 2009 23:00:00 +0100 2890460