MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Cell Transplantation' source. Sat, 20 Mar 2010 16:35:10 +0100 http://www.medworm.com/index.php?rid=3263337&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20144261%26dopt%3DAbstract Authors: Zipancic I, Calcagnotto ME, Piquer-Gil M, Mello LE, Alvarez-Dolado M Defects in GABAergic function can cause epilepsy. In the last years, cell-based therapies have attempted to correct these defects with disparate success on animal models of epilepsy. Recently, we demonstrated that medial ganglionic eminence (MGE)-derived cells grafted into the neonatal normal brain migrate and differentiate into functional mature GABAergic interneurons. These cells are able to modulate the local level of GABA-mediated synaptic inhibition, what suggest their suitability for cell-based therapies. However, it is unclear whether they can integrate in the host circuitry and rescue the loss of inhibition in pathological conditions. Thus, as proof of principle, we grafted MGE-derived cells into a mo... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3263337 Mon, 08 Feb 2010 00:00:00 +0100 3263337 http://www.medworm.com/index.php?rid=3263336&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20144262%26dopt%3DAbstract In this study, we demonstrate the direct reprogramming of a defined population of neural stem cells (NSCs) derived from the sub ventricular zone (SVZ) and adipose tissue derived cells (ADCs) from adult mice using retroviral transduction of the Yamanaka factors: Oct4, Sox2, Klf4 and c-Myc and compared the results obtained with a mouse embryonic fibroblast (mEF) control. We isolated mEFs, NSCs and ADCs from transgenic mice, which possess a GFP transgene under control of the Oct4 promoter and validated GFP expression as an indicator of reprogramming. While transduction efficiencies were not significantly different between the different cell types (mEFs 68.70+/-2.62%, ADCs 70.61+/-15.4%, NSCs, 68.72+/-3%, p=0.97), the number of GFP positive colonies and hence the number of reprogramming events... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3263336 Mon, 08 Feb 2010 00:00:00 +0100 3263336 http://www.medworm.com/index.php?rid=3195971&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20089206%26dopt%3DAbstract This study shows that targeted disruption of OLM junctional proteins enhances integration in the wildtype and degenerating retina and may be a useful approach for developing photoreceptor transplantation strategies. PMID: 20089206 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3195971 Wed, 20 Jan 2010 00:00:00 +0100 3195971 http://www.medworm.com/index.php?rid=3195966&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20089207%26dopt%3DAbstract In conclusion, Bcl-2 prolongs survival of myoblast sheets, increases production of proangiogenic paracrine mediators, and enhances the therapeutic efficacy of cell sheet transplantation. PMID: 20089207 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3195966 Wed, 20 Jan 2010 00:00:00 +0100 3195966 http://www.medworm.com/index.php?rid=3115549&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20021733%26dopt%3DAbstract Authors: Rijkelijkhuizen JK, Töns A, Terpstra OT, Bouwman E To evaluate whether further improvement in porcine islet xenotransplantation is feasible, a number of questions was addressed. Earlier we showed significant improvement in the nude mouse of the porcine islets by selection through long-term culture. Now these islets were tested in the stringent pig-to-rat model. Islets were isolated from adult pigs, cultured for 1.5-3 weeks and transplanted to rats. Possible rejection mechanisms were assessed by interference of the cellular response with cyclosporine A (CsA), blocking macrophages with gadolinium chloride (GdCl) and suppressing the humoral response with cyclophosphamide. Modifications in graft size and condition were analyzed. Untreated control recipients showed primary non... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3115549 Fri, 18 Dec 2009 00:00:00 +0100 3115549 http://www.medworm.com/index.php?rid=3115548&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20021734%26dopt%3DAbstract Authors: Kozubenko N, Turnovcova K, Kapcalova M, Butenko O, Anderova M, Rusnakova V, Kubista M, Hampl A, Jendelova P, Sykova E During the last decade, much progress has been made in developing protocols for the differentiation of human embryonic stem cells (hESCs) into a neural phenotype. The appropriate agent for cell therapy is neural precursors (NPs). Here, we demonstrate the derivation of highly enriched and expandable populations of proliferating neural precursors from the CCTL14 line of hESCs. These neural precursors could differentiate in vitro into functionally active neurons, as confirmed by immunohistochemical staining and electrophysiological analysis. Neural cells differentiated in vitro from hESCs exhibit broad cellular heterogeneity with respect to developmental stage and... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3115548 Fri, 18 Dec 2009 00:00:00 +0100 3115548 http://www.medworm.com/index.php?rid=3115547&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20021735%26dopt%3DAbstract Authors: Rocuts F, Zhang X, Yan J, Yue Y, Thomas M, Bach FH, Czismadia E, Wang H We have previously demonstrated that bilirubin administration to the recipient induces tolerance towards islet cell transplants across a complete MHC mismatch in a mouse model. Here we assess the mechanisms of such protection. Bilirubin treatment of recipients improved function of islet allografts by suppressing expressions of pro-inflammatory and pro-apoptotic genes in those islets and by increasing Foxp3 T regulatory cells (Tregs) at the site of transplanted islets at various days after transplantation. No prolongation of graft survival was observed in recipients treated with bilirubin when CD4CD25 T cells were pre-depleted from those recipients, indicating that Tregs are necessary for the protective eff... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3115547 Fri, 18 Dec 2009 00:00:00 +0100 3115547 http://www.medworm.com/index.php?rid=3115546&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20021736%26dopt%3DAbstract In this study, we have investigated the hypothesis that previously reported beneficial effect of peripheral blood mononuclear cells cultured under angiogenic conditions on cardiovascular function following ischemia is not limited to EPCs but also to monocytes contained therein. We first purified and analyzed the phenotype and secretome of human and murine blood monocytes cultured under angiogenic conditions (named MDs for monocyte derivatives) and tested their effect in a mouse model of myocardial infarction (MI). FACS analysis of MDs shows that these cells express mature endothelial cell markers and that their proliferative capacity is virtually absent consistent with their end-differentiated monocytic ontogeny. MDs secreted significant levels of HGF, IGF-1, MCP-1 and sTNFR-1 relative to ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3115546 Fri, 18 Dec 2009 00:00:00 +0100 3115546 http://www.medworm.com/index.php?rid=3076081&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19995482%26dopt%3DAbstract In conclusion, like human lipoaspirate, canine adipose tissue may also contain multipotent cells and represent an important stem cell source both for veterinary cell therapy as well as preclinical studies. PMID: 19995482 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3076081 Tue, 08 Dec 2009 00:00:00 +0100 3076081 http://www.medworm.com/index.php?rid=3076080&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19995483%26dopt%3DAbstract SEVEN CONSECUTIVE SUCCESSFUL CLINICAL ISLET ISOLATIONS WITH PANCREATIC DUCTAL INJECTION. Cell Transplant. 2009 Dec 8; Authors: Matsumoto S, Noguchi H, Shimoda M, Ikemoto T, Naziruddin B, Jackson A, Tamura Y, Greg O, Fujita Y, Chujo D, Takita M, Kobayashi N, Onaca N, Marlon L Inconsistent islet isolation is one of the issues of clinical islet transplantation. In the current study, we applied ductal injection to improve the consistency of islet isolation. Seven islet isolations were performed with the ductal injection of ET-Kyoto solution (DI group) and eight islet isolations were performed without the ductal injection (standard group) using brain-dead donor pancreata. Isolated islets were evaluated based on the Edmonton protocol for transplantation. DI group had significantly hi... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3076080 Tue, 08 Dec 2009 00:00:00 +0100 3076080 http://www.medworm.com/index.php?rid=3076079&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19995484%26dopt%3DAbstract Authors: Rabbany SY, Pastore J, Yamamoto M, Miller T, Rafii S, Aras R, Penn M Proper wound diagnosis and management is an increasingly important clinical challenge and is a large and growing unmet need. Pressure ulcers, hard-to-heal wounds, and problematic surgical incisions are emerging at increasing frequencies. At present, the wound healing industry is experiencing a paradigm shift towards innovative treatments that exploit nanotechnology, biomaterials and biologics. Our study utilized an alginate hydrogel patch to deliver Stromal-cell Derived Factor-1 (SDF-1), a naturally-occurring chemokine that is rapidly over-expressed in response to tissue injury, to assess the potential effects SDF-1 therapy on wound closure rates and scar formation. Alginate patches were loaded with either pu... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3076079 Tue, 08 Dec 2009 00:00:00 +0100 3076079 http://www.medworm.com/index.php?rid=3049348&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19951460%26dopt%3DAbstract Authors: Malavasi N, Rodrigues D, Chammas R, Chura-Chambi R, Barbuto J, Balduino K, Nonogaki S, Morganti L Endostatin (ES) is a potent inhibitor of angiogenesis and tumor growth. Continuous ES delivery of ES improves the efficacy and potency of the antitumoral therapy. The Theracyte system is a polytetrafluoroethylene (PTFE) semi-permeable membrane macroencapsulation system for implantation of genetically engineered cells specially designed for the in vivo delivery of therapeutic proteins, such as ES, which circumvents the problem of limited half-life and variation in circulating levels. In order to enable neovascularization at the tissues adjacent to the devices prior to ES secretion by the cells inside them, we designed a scheme in which empty Theracyte devices were pre-implanted s.c... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3049348 Wed, 02 Dec 2009 00:00:00 +0100 3049348 http://www.medworm.com/index.php?rid=3049347&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19951461%26dopt%3DAbstract In conclusion, we identified the best histomorphometric predictors of islet yield and posttransplant outcome. This further enhanced the predictive value of the flow cytometry analysis. These parameters should be useful for predicting islet yield and in vivo potency before clinical adult porcine islet xenotransplantation. PMID: 19951461 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3049347 Wed, 02 Dec 2009 00:00:00 +0100 3049347 http://www.medworm.com/index.php?rid=3009440&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19919732%26dopt%3DAbstract Conclusions: Altogether, results show that MSC provides a long-term superior benefit than whole BM-MNC transplantation in a rat model of chronic MI. PMID: 19919732 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3009440 Mon, 16 Nov 2009 00:00:00 +0100 3009440 http://www.medworm.com/index.php?rid=3009439&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19919733%26dopt%3DAbstract Authors: Suzuki J, Ricordi C, Chen Z A diversity of immune tolerance mechanisms have evolved to protect normal tissues from immune damage. Immune regulatory cells are critical contributors to peripheral tolerance. These regulatory cells, exemplified by the CD4Foxp3 regulatory T (Treg) cells and a recently identified population named myeloid-derived suppressor cells (MDSCs), regulate immune responses and limiting immune-mediated pathology. In a chronic inflammatory setting, such as allograft-directed immunity, there may be a dynamic "crosstalk" between the innate and adaptive immunomodulatory mechanisms for an integrated control of immune damage. CTLA4-B7-based interaction between the two branches may function as a molecular "bridge" to facilitate such "crosstalk". Understanding the int... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=3009439 Mon, 16 Nov 2009 00:00:00 +0100 3009439 http://www.medworm.com/index.php?rid=2992951&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19906330%26dopt%3DAbstract In this study the time course of homing and the body distribution of systemically delivered bone marrow mesenchymal stem cells (BM-MSCs) after myocardial infarction (MI) were evaluated. BM-MSCs were isolated from Wistar rats, expanded in vitro and their phenotypical characterization was performed by flow cytometer. Rats were randomly divided into three groups: control, sham MI, and MI. BM-MSCs (5x10) were labeled with mTc-HMPAO and injected through the tail vein 7 days after MI. Gamma camera imaging was performed at 5, 15, 30 and 60 min after cell inoculation. Due to the mTc short half-life, cell migration and location were also evaluated in heart sections using DAPI-labeled cells 7 days after transplantation. Phenotypical characterization showed that BM-MSCs were CD90, CD73, CD54 and CD45... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2992951 Tue, 10 Nov 2009 00:00:00 +0100 2992951 http://www.medworm.com/index.php?rid=2992950&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19906331%26dopt%3DAbstract This study aimed to determine the impacts of hepatocyte transplantation on the response and fate of oval cells that are activated to proliferate in acute severe hepatic injury. Retrorsine-plus- D-galactosamine (R+D-gal) treatment was used to induce acute hepatic injury and to elicit extensive activation of oval cells in male Dipeptidyl-peptidase-IV-deficient F344 rats. These rats were then randomized to receive wild-type hepatocyte transplantation or vehicle intraportally. The kinetics of oval cell response and their differentiation fate were analyzed. Results showed that oval cells were activated early and differentiated into hepatocytes in R+D-gal-treated rats without hepatocyte transplantation. With hepatocyte transplantation, the oval cells were recruited later and continued to prolife... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2992950 Tue, 10 Nov 2009 00:00:00 +0100 2992950 http://www.medworm.com/index.php?rid=2992949&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19906332%26dopt%3DAbstract In conclusion, our results suggest that grafted hMSCs exert neuroprotective effects against nigrostriatal degeneration induced by 6-OHDA. The mechanisms underlying this effect remain to be clarified, although it is likely that the acquisition of a glial phenotype by grafted hMSCs may lead to the release of pro-survival cytokines within the lesioned striatum. PMID: 19906332 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2992949 Tue, 10 Nov 2009 00:00:00 +0100 2992949 http://www.medworm.com/index.php?rid=2992948&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19906333%26dopt%3DAbstract Authors: Borlongan CV, Parolini O [no abstract]. PMID: 19906333 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2992948 Tue, 10 Nov 2009 00:00:00 +0100 2992948 http://www.medworm.com/index.php?rid=2955147&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19878623%26dopt%3DAbstract In conclusion, high frequency of proteinuria was observed after ITx. However, it seems to be milder and have less impact on KF when albumin is not the major source of proteinuria. Prospective evaluation of proteinuria, including tubular function markers, should be performed to elucidate the mechanisms of kidney damage in this population. PMID: 19878623 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2955147 Thu, 29 Oct 2009 00:00:00 +0100 2955147 http://www.medworm.com/index.php?rid=2955146&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19878624%26dopt%3DAbstract CONCLUSION: The binding of C2A-ESCs to moribund cardiomyocytes demonstrates that the retention of transplanted cells may be improved by conferring these cells with the ability to bind infarcted tissue. Once established, this novel approach may be applicable to other types of transplanted therapeutic cells. PMID: 19878624 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2955146 Thu, 29 Oct 2009 00:00:00 +0100 2955146 http://www.medworm.com/index.php?rid=2955145&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19878625%26dopt%3DAbstract Authors: Petersen TH, Hitchcock T, Muto A, Calle EA, Zhao L, Gong Z, Gui L, Dardik A, Bowles DE, Counter CM, Niklason LE While advances in regenerative medicine and vascular tissue engineering have been substantial in recent years, important stumbling blocks remain. In particular, the limited lifespan of differentiated cells that are harvested from elderly human donors is an important limitation in many areas of regenerative medicine. Recently, a mutant of the human telomerase reverse transcriptase enzyme (TERT) was described, which is highly processive and elongates telomeres more rapidly than conventional telomerase. This mutant, called pot1-TERT, is a chimeric fusion between the DNA binding protein pot1 and TERT. Because pot1-TERT is highly processive, it is possible that transient ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2955145 Thu, 29 Oct 2009 00:00:00 +0100 2955145 http://www.medworm.com/index.php?rid=2955144&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19878626%26dopt%3DAbstract Authors: Boddington SE, Md TD, Md PJ, Schlieve CR, Mandrussow L, Phd DD, Phd HS, Ritner C, Md DG, Phd YL, Phd SZ, Phd HE Human embryonic stem cell derived cardiomyocytes (hESC-CMs) have demonstrated the ability to improve myocardial function following transplantation into an ischemic heart; however the functional benefits are transient possibly due to poor cell retention. A diagnostic technique that could visualize transplanted hESC-CMs could help to optimize stem cell delivery techniques. Thus, the purpose of this study was to develop a labeling technique for hESCs and hESC-CMs with the FDA-approved contrast agent Indocyanine Green (ICG) for optical imaging (OI). hESCs were labeled with 0.5, 1.0, 2.0 and 2.5 mg/ml of ICG for 30, 45 and 60 minutes at 37 degrees C. Longitudinal OI studi... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2955144 Thu, 29 Oct 2009 00:00:00 +0100 2955144 http://www.medworm.com/index.php?rid=2955143&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19878627%26dopt%3DAbstract Authors: Markway BD, Tan GK, Brooke G, Hudson JE, Cooper-White JJ, Doran MR Chondrogenesis of mesenchymal stem cells (MSCs) is typically induced when they are condensed into a single aggregate and exposed to transforming growth factor beta (TGF-beta). Hypoxia, like aggregation and TGF-beta delivery, may be crucial for complete chondrogenesis. However, the pellet dimensions and associated self-induced oxygen gradients of current chondrogenic methods may limit the effectiveness of in vitro differentiation and subsequent therapeutic uses. Here we describe the use of embryoid body-forming technology to produce microscopic aggregates of human bone marrow MSCs (BM-MSCs) for chondrogenesis. The use of micropellets reduces the formation of gradients within the aggregates resulting in a more ho... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2955143 Thu, 29 Oct 2009 00:00:00 +0100 2955143 http://www.medworm.com/index.php?rid=2955142&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19878628%26dopt%3DAbstract Authors: Richard PL, Gosselin C, Laliberté T, Paradis M, Goulet M, Tremblay JP, Skuk D Intramuscular cell transplantation in humans requires so far meticulous repetitive cell-injections. Performed percutaneously with syringes operated manually, the procedure is very time-consuming and requires a lot of concentration to deliver the cells exactly in the required region. This becomes impractical and inaccurate for large volumes of muscle. In order to accelerate this task, to render it more precise and to perform injections more reproducible in large volumes of muscle, we developed a specific semi-manual device for intramuscular repetitive cell injections. Our prototype delivers very small quantities of cell suspension, homogeneously throughout several needles, from a container in the... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2955142 Thu, 29 Oct 2009 00:00:00 +0100 2955142 http://www.medworm.com/index.php?rid=2888604&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19818205%26dopt%3DAbstract Authors: Mandai M, Ikeda H, Jin ZB, Iseki K, Ishigami C, Takahashi M Using the mouse ES cell line with green fluorescent protein knocked-in at the Rx locus (Rx-KI ES cell), we previously showed that photoreceptors can be efficiently obtained in defined culture conditions by enriching Rx-positive retinal progenitor cells. We aimed to explore a protocol applicable for non-Rx-labeled stem cell lines for subsequent enrichment of retinal photoreceptor precursors for transplantation. The Rx-KI ES cell line was differentiated according to the serum-free suspension conditions with serum-free Suspension /Dkk1/LeftyA/serum/activin method (SFEB/DLFA) described previously. Enrichment efficacy by negative selection was compared among 20 different lectins and the lectin combination that effectively ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2888604 Thu, 08 Oct 2009 23:00:00 +0100 2888604 http://www.medworm.com/index.php?rid=2888603&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19818206%26dopt%3DAbstract We report here that the combination of the NT-3, PDGF and fibrin scaffold (with or without HBDS) enhanced the total number of ESNPCs present in the spinal cord lesion 2 weeks after injury. In addition, the inclusion of the HBDS with growth factor resulted in an increase in the number of ESNPC-derived NeuN positive neurons. These results demonstrate the ability of fibrin scaffolds and the controlled release of growth factors to enhance the survival and differentiation of neural progenitor cells following transplantation into a SCI model. PMID: 19818206 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2888603 Thu, 08 Oct 2009 23:00:00 +0100 2888603 http://www.medworm.com/index.php?rid=2888602&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19818207%26dopt%3DAbstract Conclusion: Our study shows that both purified CD133+ stem cells and myeloid mononuclear cells, either IM- or IV-administered, have similar neoangiogenic ability. Nevertheless, transdifferentiation into endothelial cells is not the mechanism responsible for their beneficial effect. PMID: 19818207 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2888602 Thu, 08 Oct 2009 23:00:00 +0100 2888602 http://www.medworm.com/index.php?rid=2888601&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19818208%26dopt%3DAbstract Authors: Brandhorst H, Friberg A, Nilsson B, Andersson HH, Felldin M, Foss A, Salmela K, Tibell A, Tufveson G, Korsgren O, Brandhorst D For more than a decade Liberase HI was commonly used as the standard enzyme blend for clinical human islet isolation until enforced replacement by collagenase NB1 (NB1). This change resulted initially in a reduction in islet isolation outcome and transplant activities worldwide. This retrospective study was initiated to compare the efficiency of NB1 premium grade with Liberase in 197 human islet isolations. All pancreata were processed between January 2006 and June 2008 utilizing the same procedures for isolation and quality assessment except the administration of preselected lots of either Liberase (n = 101) or NB1 (n = 96). Utilizing Liberase signifi... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2888601 Thu, 08 Oct 2009 23:00:00 +0100 2888601 http://www.medworm.com/index.php?rid=2888600&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19818209%26dopt%3DAbstract Authors: Buchwald P, Wang X, Khan A, Bernal A, Fraker C, Inverardi L, Ricordi C The ability to consistently and reliably assess the total number and the size distribution of isolated pancreatic islet cells from a small sample is of crucial relevance for the adequate characterization of islet cell preparations used for research or transplantation purposes. Here, data from a large number of isolations were used to establish a continuous probability density function describing the size distribution of human pancreatic islets. This function was then used to generate a polymeric microsphere mixture with a composition resembling those of isolated islets, which, in turn, was used to quantitatively assess the accuracy, reliability, and operator-dependent variability of the currently utilized m... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2888600 Thu, 08 Oct 2009 23:00:00 +0100 2888600 http://www.medworm.com/index.php?rid=2859332&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19796495%26dopt%3DAbstract Authors: Glavaski-Joksimovic A, Virag T, Chang QA, West NC, Mangatu TA, McGrogan MP, Dugich-Djordjevic M, Bohn MC Parkinson's disease (PD) is a common neurodegenerative disease characterized by theselective loss of dopaminergic (DA) neurons in the midbrain. Various types of stem cells that have potential to differentiate into DA neurons are being investigated as cellular therapies for PD. Stem cells also secrete growth factors and therefore may also have therapeutic effects in promoting the health of diseased DA neurons in the PD brain. To address this possibility in an experimental model of PD, bone marrow-derived neuroprogenitor-like cells were generated from bone marrow procured from healthy human adult volunteers and their potential to elicit recovery of damaged DA axons was studie... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2859332 Sun, 27 Sep 2009 23:00:00 +0100 2859332 http://www.medworm.com/index.php?rid=2859331&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19796496%26dopt%3DAbstract Authors: Meyburg J, Alexandrova K, Barthold M, Kafert-Kasting S, Schneider AS, Attaran M, Hoerster F, Schmidt J, Hoffmann GF, Ott M Liver cell transplantation (LCT) is a very promising method for the use in pediatric patients. It is significantly less invasive than whole organ transplantation, but has the potential to cure or at least to substantially improve severe disorders like inborn errors of metabolism or acute liver failure. Prior to a widespread use of the technique in children, some important issues regarding safety and efficacy must be addressed. We developed a mathematical model to estimate total hepatocyte counts in relation to bodyweight to make possible more appropriate dose calculations. Different liver cell suspensions were studied at different flow rates and different ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2859331 Sun, 27 Sep 2009 23:00:00 +0100 2859331 http://www.medworm.com/index.php?rid=2859330&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19796497%26dopt%3DAbstract Authors: Group TC As of October 1, 2007, 25 North American medical institutions and one European islet transplant center reported detailed information to the Registry on 315 allograft recipients; of which 285 were islet alone (IA) and 30 were islet after kidney. Of the 114 IA recipients expected at four years after their last infusion, 12% were insulin independent, 16% were insulin dependent with detectable C-peptide, 40% had no detectable C-peptide, and 32% had missing C-peptide data or were lost to follow-up. Of the IA recipients, 72% achieved insulin independence at least once over three years and multiple infusions. Factors associated with achievement of insulin independence included islet size >1.0 expressed as IEQs per islet number (hazard ratio, HR=1.5 p=0.06), additional inf... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2859330 Sun, 27 Sep 2009 23:00:00 +0100 2859330 http://www.medworm.com/index.php?rid=2859329&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19796498%26dopt%3DAbstract Conclusions: We demonstrated for the first time that direct MSCs-rBMECs interaction stimulated the endothelial differentiation of MSCs via TACE/TNF alpha signaling. PMID: 19796498 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2859329 Sun, 27 Sep 2009 23:00:00 +0100 2859329 http://www.medworm.com/index.php?rid=2859328&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19796499%26dopt%3DAbstract Authors: Chopp M, Steinberg GK, Kondziolka D, Lu M, Bliss TM, Li Y, Hess DC, Borlongan CV A consortium of translational stem cell and stroke experts from multiple academic institutes and biotechnology companies, under the guidance of the government (FDA/NIH), is missing. Here, we build a case for the establishment of this consortium if cell therapy for stroke is to advance from the laboratory to the clinic. PMID: 19796499 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2859328 Sun, 27 Sep 2009 23:00:00 +0100 2859328 http://www.medworm.com/index.php?rid=2859327&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19796500%26dopt%3DAbstract Authors: Sladek JR, Sanberg PR Cell therapy for stroke may benefit from translational research guidelines to aid its entry into the clinic as a safe and effective treatment. PMID: 19796500 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2859327 Sun, 27 Sep 2009 23:00:00 +0100 2859327 http://www.medworm.com/index.php?rid=2859326&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19796501%26dopt%3DAbstract TNF, PIG CD86 AND VCAM-1 IDENTIFIED AS POTENTIAL TARGETS FOR INTERVENTION IN XENOTRANSPLANTATION OF PIG CHONDROCYTES. Cell Transplant. 2009 Sep 28; Authors: Sommaggio R, Máñez R, Costa C Xenotransplantation of genetically engineered porcine chondrocytes may benefit many patients who suffer cartilage defects. In this work, we sought to elucidate the molecular bases of the cellular response to xenogeneic cartilage. To this end, we isolated pig costal chondrocytes (PCC) and conducted a series of functional studies. First, we determined by flow cytometry the cell surface expression of multiple immunoregulatory proteins in resting conditions or after treatment with human TNFalpha, IL-1alpha or IL-1beta, which did not induce apoptosis. TNFalpha and to a lesser extent IL-1al... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2859326 Sun, 27 Sep 2009 23:00:00 +0100 2859326 http://www.medworm.com/index.php?rid=2859325&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19796502%26dopt%3DAbstract Authors: A BC, Mt D, A L, E P, J M, Jv C, Mj GL The first indication of hepatocyte transplantation is inborn liver-based metabolic disorders and among which, urea cycle disorders leading to the impairment to detoxify ammonia and Crigler-Najjar Syndrome type I, a deficiency in the hepatic UDP-glucuronosyltransferase 1A1, present the highest incidence. Metabolically qualified human hepatocytes are required for clinical infusion. We proposed fast and sensitive procedures to determine their suitability for transplantation. For this purpose, viability, attachment efficiency and metabolic functionality (ureogenic capability, cytochrome P450 and phase II activities) are assayed prior to clinical cell infusion to determine the quality of hepatocytes. Moreover, the evaluation of urea synthesis ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2859325 Sun, 27 Sep 2009 23:00:00 +0100 2859325 http://www.medworm.com/index.php?rid=2847436&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19785934%26dopt%3DAbstract This study was designed to investigate functional recovery after the transplantation of mesenchymal stem cells (MSCs) or neurally differentiated MSCs (NMSCs) derived from bone marrow in a rat model of spinal cord injury (SCI). Sprague-Dawley rats were subjected to incomplete SCI using an NYU impactor to create a free drop contusion at the T9 level. The SCI rats were then classified into three groups; MSCs, NMSCs and phosphated buffered saline (PBS)-treated groups. The cells or PBS were administrated 1 week after SCI. Basso-Beattie-Bresnahan (BBB) locomotor rating scores were measured at 1-week intervals for 9 weeks. Somatosensory evoked potentials (SSEPs) and motor evoked potentials (MEPs) were also recorded 8 weeks after transplantation. While transplantation of MSCs led to a clear tenden... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2847436 Sun, 27 Sep 2009 23:00:00 +0100 2847436 http://www.medworm.com/index.php?rid=2847435&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19785935%26dopt%3DAbstract Authors: Vergani A, Clissi B, Sanvito F, Doglioni C, Fiorina P, Pardi R Innovative tolerogenic protocols in transplantation would take advantage of the development of new tools capable of evaluating the impact of these treatments on immune system. These assays have potential for clinical application. Currently, many of these studies are based on the analysis of peripheral lymph nodes and blood-derived cells, where the percentage of alloantigen-specific cells can be low or even unpredictable. We combined a Laser Capture Microdissection (LCM) technique with Real-Time PCR (RT-PCR) to evaluate gene profile of islet infiltrating lymphocytes. Donor Lewis rats islets were transplanted under the kidney capsule in diabetic Brown Norway rats. Administration of anti-LFA1 mAb or anti-CD28 F(Ab)' w... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2847435 Sun, 27 Sep 2009 23:00:00 +0100 2847435 http://www.medworm.com/index.php?rid=2847434&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19785936%26dopt%3DAbstract Authors: Burghoff S, Ding Z, Blaszczyk A, Wirrwar A, Buchholz D, Müller HW, Schrader J Transplantation of human endothelial progenitor cells (EPCs) may improve vascularization and left ventricular function after myocardial infarction. The scope of this study was to explore, whether crosslinking of EPCs may enhance the deposition of cells in the rat heart after clinical-like, intracoronary transplantation. To this end, (1)(1)(1)Indium-oxinate labelled human EPCs were infused by a minimally invasive technique into the coronary arteries of immuno-suppressed Wistar rats under control conditions and after ischemia-reperfusion. In a second set of experiments hEPCs were treated with phytohemagglutinin to create small cell clusters prior to transplantation. Continous 3-dimensional HiSPECT... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2847434 Sun, 27 Sep 2009 23:00:00 +0100 2847434 http://www.medworm.com/index.php?rid=2847433&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19785937%26dopt%3DAbstract Authors: Gao LR, Xu RY, Zhang NK, Chen Y, Wang ZG, Zhu ZM, Fei YX, Cao Y, Xu HT, Yang AY We previously reported that intracoronary implantation of bone marrow mononuclear cells (BMMC) into ischemic hearts improved cardiac function after myocardial infarction. However, the mechanisms have not been elucidated. The present study investigates whether apelin, a newly described inotropic peptide with important cardiovascular regulatory properties, contributes to the functional improvement in patients with severe heart failure after cell transplantation. Forty consecutive patients with severe heart failure secondary to myocardial infarction were assigned to the BMMC therapy group or the standard medication group according to each patient's decision on a signed consent document. In 20 patients... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2847433 Sun, 27 Sep 2009 23:00:00 +0100 2847433 http://www.medworm.com/index.php?rid=2832602&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775507%26dopt%3DAbstract Authors: Kobayashi N PMID: 19775507 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832602 Fri, 25 Sep 2009 23:08:03 +0100 2832602 http://www.medworm.com/index.php?rid=2832601&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775508%26dopt%3DAbstract Authors: Kobayashi N, Yuasa T, Okitsu T In diabetes, a loss of pancreatic beta-cells causes insulin dependency. When insulin dependency is caused by type 1 diabetes or pancreatic diabetes, for example, pancreatic beta-cells need to be regenerated for definitive treatment. The methods for generating pancreatic beta-cells include a method of creating pancreatic beta-cells in vitro and implanting them into the body and a method of regenerating pancreatic beta-cells in the body via gene introduction or the administration of differential proliferation factors to the body. Moreover, the number of pancreatic beta-cells is also low in type 2 diabetes, caused by the compounding factors of insulin secretory failure and insulin resistance; therefore, if pancreatic beta-cells can be regenerated in... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832601 Fri, 25 Sep 2009 23:08:03 +0100 2832601 http://www.medworm.com/index.php?rid=2832600&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775509%26dopt%3DAbstract Authors: Noguchi H, Levy MF, Kobayashi N, Matsumoto S A large number of reports have shown that the two-layer method (TLM), which employs oxygenated perfluorochemical (PFC) and University of Wisconsin (UW) solution, is superior to simple cold storage in UW in islet transplantation. However, two recent large-scale studies showed no beneficial effect of TLM compared with UW storage in human islet transplantation. We reevaluated the effect of TLM by following three groups: group 1: UW simple storage; group 2: TLM performed by multiorgan procurement teams (not specialists of islet isolation); and group 3: TLM performed by specialists of islet isolation (Noguchi and Matsumoto). There were no significant differences between groups 1 and 2, whereas islet yields were significantly higher in gr... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832600 Fri, 25 Sep 2009 23:08:03 +0100 2832600 http://www.medworm.com/index.php?rid=2832599&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775510%26dopt%3DAbstract Authors: Bae JY, Matsumura K, Wakitani S, Kawaguchi A, Tsutsumi S, Hyon SH A fresh osteochondral allograft is one of the most effective treatments for cartilage defects of the knee. Despite the clinical success, fresh osteochondral allografts have great limitations in relation to the short storage time that cartilage tissues can be well-preserved. Fresh osteochondral grafts are generally stored in culture medium at 4 degrees C. While the viability of articular cartilage stored in culture medium is significantly diminished within 1 week, appropriate serology testing to minimize the chances for the disease transmission requires a minimum of 2 weeks. (-)-Epigallocatechin-3-O-gallate (EGCG) has differential effects on the proliferation of cancer and normal cells, thus a cytotoxic effect on... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832599 Fri, 25 Sep 2009 23:08:03 +0100 2832599 http://www.medworm.com/index.php?rid=2832598&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775511%26dopt%3DAbstract This study examined the effects of EGCG on skin cryopreservation. Skin sample biopsy specimens from GFP rats were previously treated with/without EGCG then moved to -196 degrees C. Skin samples were transplanted to nude mice after 2, 8, and 24 weeks of preservation. Glucose consumption was measured after thawing to assess the metabolic activity. Two weeks later the transplanted skin grafts were excised and histologically analyzed. Histological examinations revealed the degeneration of the epidermal and dermal layers in all groups. In the EGCG groups, the grafts showed higher integrity in the epidermal layer and dermal matrix. The present findings suggest the future clinical usefulness of EGCG for skin preservation; however, the mechanism by which EGCG promotes skin preservation still remai... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832598 Fri, 25 Sep 2009 23:08:03 +0100 2832598 http://www.medworm.com/index.php?rid=2832597&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775512%26dopt%3DAbstract Authors: Matsumura K, Takayama H, Bae JY, Kurihara M, Tsutsumi S, Hyon SH The effect of epigallocatechin-3-O-gallate (EGCG), a major component of green tea, on platelet preservation was evaluated. Single donor platelets (N = 10) were collected and preserved by the standard method. EGCG was added to the platelet concentrates before preservation and then the functional and biochemical parameters were monitored throughout the storage period. After 6 days of preservation, the aggregability of the platelets was significantly maintained by addition of 50 and 100 microg/ml of EGCG. Platelet prothrombinase activity was also significantly retained by the addition of EGCG. The accumulation of P-selectin and RANTES in the plasma preserved with EGCG was less than those preserved without EGCG, whic... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832597 Fri, 25 Sep 2009 23:08:03 +0100 2832597 http://www.medworm.com/index.php?rid=2832596&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775513%26dopt%3DAbstract Authors: Hatayama N, Yoshida Y, Seki K Krebs-Henseleit (KH) solution was used to fill the heart chamber of an isolated rat heart before it was immersed in perfluorocarbon (PFC), which is an inert fluid. A gas mixture (PCO(2) = 150 hPa and PO(2) = 850 hPa) was then aerated at a constant rate into the PFC solution, and the isolated heart was thereafter preserved for 96 h with KH solution perfused continuously at a rate of 0.1 ml/h from the aorta of the isolated heart through a cannula. After preservation, the preserved heart was heterotopically transplanted into the neck of a recipient rat and then it was resuscitated. Using this method for preserving mammalian organs, we attained reproducibility after perfusion preservation for 96 h. PMID: 19775513 [PubMed - in process] (Source: Cel... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832596 Fri, 25 Sep 2009 23:08:03 +0100 2832596 http://www.medworm.com/index.php?rid=2832595&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775514%26dopt%3DAbstract This report demonstrates that significant reproducibility has been verified. The heart was removed from the refrigerator 24 h later and heterotopic heart transplantation was performed in the right neck of a recipient rat and the pulsating of the transplanted heart was detected by an electrocardiogram. PMID: 19775514 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832595 Fri, 25 Sep 2009 23:08:03 +0100 2832595 http://www.medworm.com/index.php?rid=2832594&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775515%26dopt%3DAbstract In this study, we compared ulinastatin with another trypsin inhibitor, Pefabloc, in preservation solution for islet isolation. Islet yield before purification was higher in the MK/PFC group compared with the ET-Kyoto with Pefabloc (PK)/PFC group. The stimulation index was higher for the MK/PFC group than for the PK/PFC group. These data suggest that ET-Kyoto with ulinastatin was the better combination for pancreas preservation than ET-Kyoto with Pefabloc. Based on these data, we now use ET-Kyoto solution with ulinastatin for clinical islet transplantation. PMID: 19775515 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832594 Fri, 25 Sep 2009 23:08:03 +0100 2832594 http://www.medworm.com/index.php?rid=2832593&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775516%26dopt%3DAbstract In this study, we applied the KIIM for brain-dead donors and reevaluated donor usability based on the Japanese islet donor criteria. Islets were isolated with the Ricordi method using pancreata stored in University of Wisconsin (UW) solution (UW group) or by the two-layer method (TLM group) or the TLM combined with ductal injection (DI group). We implemented the KIIM (KIIM group) to confirm the effect of the KIIM on brain-dead donors. Donor charts in Texas from 2005 to 2006 were reviewed. If pancreata were not used clinically, the reason was reviewed and donors were reevaluated based on Japanese criteria. There were no significant differences of islet yield, viability, and purity between the UW and TLM groups. The DI group significantly improved islet yields and isolations were further imp... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832593 Fri, 25 Sep 2009 23:08:03 +0100 2832593 http://www.medworm.com/index.php?rid=2832592&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775517%26dopt%3DAbstract In this study, we evaluated the SUITO index for the prediction of clinical outcome after single islet transplantation. Single islet transplantations were performed into six type 1 diabetic patients. Isolated islets were quantitatively assessed at the time of transplantation. The SUITO index was calculated as follows: fasting C-peptide (ng/dl)/[fasting blood glucose (mg/dl) - 63] x 1500. Islet yield/recipient's body weight and SUITO index were evaluated, along with HbA(1C), relative insulin dose (insulin dose posttransplant/pretransplant), and M-values. HbA(1C) improved in all cases, irrespective of the SUITO index score or islet yield/body weight. The average SUITO index from postoperative days 3 to 30 (R(2) = 0.728, p < 0.04), but not islet yield/body weight (R(2) = 0.259, p = 0.303), ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832592 Fri, 25 Sep 2009 23:08:03 +0100 2832592 http://www.medworm.com/index.php?rid=2832591&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775518%26dopt%3DAbstract In this study, we established a mouse pancreatic stem cell line without genetic manipulation. The duct-rich population after islet isolation was inoculated into 96-well plates in limiting dilution. From over 200 clones, 15 clones were able to be cultured for over 3 months. The HN#13 cells, which had the highest expression of insulin mRNA after induction, expressed PDX-1 transcription factor, glucagon-like peptide-1 (GLP-1) receptor, and cytokeratin-19 (duct-like cells). These cells continue to divide actively beyond the population doubling level (PDL) of 300. Exendin-4 treatment and transduction of PDX-1 and NeuroD proteins by protein transduction technology in HN#13 cells induced insulin and pancreas-related gene expression. This cell line could be useful for analyzing pancreatic stem cel... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832591 Fri, 25 Sep 2009 23:08:03 +0100 2832591 http://www.medworm.com/index.php?rid=2832590&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775519%26dopt%3DAbstract In this study, we investigated the in vitro gene transfer efficiency of Sendai virus (SeV) vectors, a paramyxovirus vector that can efficiently introduce foreign genes without toxicity into several cell types, including pancreatic stem cells. The dose-dependent GFP expression of pancreatic stem cells transfected with SeV vectors after 48 h of culture at 37 degrees C was observed. The transfection of pancreatic stem cells with SeV vectors and AdV vectors results in equal expression of the transgene (GFP expression) in the cells after 48 h of culture at 37 degrees C. Although the transfection of pancreatic stem cells with AdV vectors at high MOIs was cytotoxic, transfection with SeV vectors at high MOIs was rarely cytotoxic. In addition, pancreatic stem cells transfected with SeV maintained ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832590 Fri, 25 Sep 2009 23:08:03 +0100 2832590 http://www.medworm.com/index.php?rid=2832589&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775520%26dopt%3DAbstract In this study, we investigated the transdifferentiation potential of somatic stem cells using adipose tissue-derived stem/progenitor cells (ASCs; mesodermal stem cells) and pancreatic stem cells (endodermal stem cells). Previous reports from other groups describe the protocol that has been used to differentiate ASCs or mesenchymal stem cells (MSCs) in bone marrow into insulin-producing cells. Induction 1: ASCs were cultured for 3 days in ultra-low attachment plates under serum-free conditions. Induction 2: ASCs were cultured for 24 h with L-DMEM, and reinduced with serum-free H-DMEM for another 10 h. Unlike previous reports, we did not get ASCs to express any pancreas-specific genes, including insulin-1 or insulin-2. Pancreatic stem cells were induced to differentiate into adipo/osteogenic... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832589 Fri, 25 Sep 2009 23:08:03 +0100 2832589 http://www.medworm.com/index.php?rid=2832588&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775521%26dopt%3DAbstract In this study, negatively charged QDs655 were applied for ASCs labeling, with the cationic liposome, Lipofectamine. The cytotoxicity of QDs655-Lipofectamine was assessed for ASCs. Although some cytotoxicity was observed in ASCs transfected with more than 2.0 nM of QDs655, none was observed with less than 0.8 nM. To evaluate the time dependency, the fluorescent intensity with QDs655 was observed until 24 h after transfection. The fluorescent intensity gradually increased until 2 h at the concentrations of 0.2 and 0.4 nM, while the intensity increased until 4 h at 0.8 nM. The ASCs were differentiated into both adipogenic and osteogenic cells with red fluorescence after transfection with QDs655, thus suggesting that the cells retain their potential for differentiation even after transfected w... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832588 Fri, 25 Sep 2009 23:08:03 +0100 2832588 http://www.medworm.com/index.php?rid=2832587&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775522%26dopt%3DAbstract This study assessed the gene transfer efficiency to floating ASCs with SeV vectors. Although a slight cytotoxicity was observed, the efficiency of gene transfer to cells in the floating state was much higher at all times and all concentrations at MOIs of 2, 10, and 20 than in the adhesion state. Moreover, ASCs transfected with SeV vectors in floating state have the same potential for their differentiation into specific tissues, such as adipocytes and osteocytes, as untransfected ASCs. These data suggest that SeV transfection to ASCs in the floating state could therefore be useful for gene transfer technology. PMID: 19775522 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832587 Fri, 25 Sep 2009 23:08:03 +0100 2832587 http://www.medworm.com/index.php?rid=2832586&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775523%26dopt%3DAbstract In conclusion, the transplantation of ASCs in combination with heparin was thus found to effectively treat acute liver failure. PMID: 19775523 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832586 Fri, 25 Sep 2009 23:08:03 +0100 2832586 http://www.medworm.com/index.php?rid=2832585&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775524%26dopt%3DAbstract Authors: Miyamoto Y, Enosawa S, Takeuchi T, Takezawa T Cryopreservation is generally performed on cells in suspension. In the case of adherent cells such as hepatocytes, a loss of their ability to attach is a more serious problem than a decreased viability after cryopreservation. We herein report a novel technology of direct in situ cryopreservation of cells cultured on collagen vitrigel membranes, which have excellent mechanical strength and can be easily handled by tweezers even when coated with cultured cells. Rat primary hepatocytes, mitomycin C-treated mouse fibroblasts (feeder cells for ES cells), and mouse ES cells on the feeder cells were cultured on collagen vitrigel membranes for 1 day. The membranes with cells attached were then plucked up from the dish, soaked in cryopreser... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832585 Fri, 25 Sep 2009 23:08:03 +0100 2832585 http://www.medworm.com/index.php?rid=2832584&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775525%26dopt%3DAbstract Authors: Kitazawa Y, Fujino M, Li XK, Xie L, Ichimaru N, Okumi M, Nonomura N, Tsujimura A, Isaka Y, Kimura H, Hünig T, Takahara S Regulatory lymphocytes play a pivotal role in preventing organ-specific autoimmune disease and in induction and maintenance of tolerance in various experimental transplantation models. The enhancement of the number and activity of peripheral CD4(+)CD25(+) Treg cells is an obvious goal for the treatment of autoimmunity and for the suppression of alloreactions. The present study demonstrates that naturally occurring CD4(+)CD25(+) Treg (nTreg) cells preferentially proliferate to a fourfold increase within 3 days in response to the administration of a single superagonistic CD28-specific monoclonal antibody (supCD28 mAb). The appearance of increased Foxp3 mo... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832584 Fri, 25 Sep 2009 23:08:03 +0100 2832584 http://www.medworm.com/index.php?rid=2832583&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775526%26dopt%3DAbstract Authors: Kihara Y, Matsuno N, Mijiti A, Nagao T, Takeuchi H, Unezaki S, Hirano T The in vitro response of peripheral blood mononuclear cells (PBMCs) to the suppressive effects of calcineurin inhibitors is known to correlate with the clinical efficacy of drugs used in renal transplantations. The present study was conducted to examine the differences of PBMC responses to calcineurin inhibitors between chronic renal failure (CRF) patients awaiting renal transplantation and cirrhosis patients awaiting liver transplantation. The study included 99 CRF patients awaiting renal transplantation and 27 cirrhosis patients awaiting liver transplantation. Twenty milliliters of venous blood was taken 1-7 days before transplantation. The in vitro drug concentrations giving 50% inhibition of PBMC blast... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832583 Fri, 25 Sep 2009 23:08:03 +0100 2832583 http://www.medworm.com/index.php?rid=2832582&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775527%26dopt%3DAbstract Authors: Kusaka M, Kuroyanagi Y, Mori T, Nagaoka K, Sasaki H, Maruyama T, Hayakawa K, Shiroki R, Kurahashi H, Hoshinaga K Because of the worldwide shortage of renal grafts, kidney transplantation (KTx) from donors after cardiac death (DCD) is an alternative way to obtain KTx from brain-dead donors. Although the prognosis of DCD KTx is gradually improving, the graft often undergoes delayed graft function (DGF), rendering the control of DGF essential for post-KTx patient care. In an attempt to characterize etiology of DGF, genome-wide gene expression profiling was performed using renal biopsy samples performed at 1 h after KTx from DCD and the data were compared with those of KTx from living donors (LD). A total of 526 genes were differentially expressed between them. Genes involved in a... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832582 Fri, 25 Sep 2009 23:08:03 +0100 2832582 http://www.medworm.com/index.php?rid=2832581&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775528%26dopt%3DAbstract Authors: Mijiti A, Matsuno N, Takeuchi H, Unezaki S, Nagao T, Hirano T Successful immunosuppressive therapy is critical for liver transplantation; however, a considerable number of patients experience fatal rejection or alternatively exhibit serious infection resulting from excessive immunosuppression. The in vitro tacrolimus response of peripheral blood mononuclear cells (PBMCs) before transplantation was compared to the clinical outcome up to 4 weeks after operation in 28 living-donor liver transplant recipients treated with tacrolimus. The tacrolimus IC(50) values against concanavalin A-induced PBMC blastogenesis in vitro were calculated. These recipients were classified into two groups with the mean tacrolimus IC(50) (0.18 ng/ml) as the cutoff point, after which the clinical outcom... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832581 Fri, 25 Sep 2009 23:08:03 +0100 2832581 http://www.medworm.com/index.php?rid=2832580&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775529%26dopt%3DAbstract In this study, we compared the growth of f-hepatocytes and hepatocytes from normal livers (n-hepatocytes). Hepatocytes were isolated from normal and CCl(4)-treated wild-type Fischer rats that express dipeptidyl dipeptidase IV (DPPIV) gene (DPPIV(+)). The n- and f-hepatocytes proliferated in culture at similar rates. Both types of hepatocytes were transplanted into DPPIV(-) mutant Fischer rats that had been treated with retrorsine to injure the liver and were partially hepatectomized (PHx) before transplantation. Both n- and f-DPPIV(+)-hepatocytes proliferated and formed colonies. The colony sizes of f-hepatocytes 21 days posttransplantation were approximately three times those of n-hepatocytes. The hepatocytes were analyzed using a fluorescence activated cell sorter (FACS). The FACS profil... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832580 Fri, 25 Sep 2009 23:08:03 +0100 2832580 http://www.medworm.com/index.php?rid=2832579&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775530%26dopt%3DAbstract Authors: Miyamoto Y, Ikeya T, Enosawa S Three-dimensional culture procedures have attracted attention in various fields of cell biology. A newly developed cell array assisted in the formation of hepatocyte spheroids by two innovations: 1) micropatterning by a hydrophilic polymer, and 2) the use of bovine carotid artery-derived HH cells as feeder cells. The former contributes to the standardization of the spheroid size and the latter to the maintenance of the spheroids. We created a way to provide a ready-to-use cell array by cryopreservation of an HH feeder cell cultured array. After inoculation of HH cells on the cell array, the culture medium was replaced by freezing medium containing dimethyl sulfoxide. Thereafter, the array was frozen and stored in a -80 degrees C deep freezer. At ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832579 Fri, 25 Sep 2009 23:08:03 +0100 2832579 http://www.medworm.com/index.php?rid=2832578&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19775531%26dopt%3DAbstract Authors: Yuasa T, Rivas-Carrillo JD, Navarro-Alvarez N, Soto-Gutierrez A, Kubota Y, Tabata Y, Okitsu T, Noguchi H, Matsumoto S, Nakaji S, Tanaka N, Kobayashi N The development of a bioartificial pancreas (BAP) with immunoisolating fashion has been gaining attention as a new method for treating diabetes. We have been proceeding with the development of a bag-type BAP that can be easily implanted and that allows for the optional injection or rejection of cells at any time. If fibrosis develops around a BAP device, then the permeability of substances transmitted through a semipermeable membrane will decrease, thereby reducing the reactivity with glucose, so it is necessary for the material of the device to have an excellent histocompatibility. Furthermore, in order to improve the efficacy ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2832578 Fri, 25 Sep 2009 23:08:03 +0100 2832578 http://www.medworm.com/index.php?rid=2682636&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19660173%26dopt%3DAbstract Conclusion: Autologous PRP and bilayer collagen matrix stimulated the formation of cartilage tissues. The findings implicated that the combination of PRP with collagen matrix may repair larger cartilage defects which currently require the complex autologous chondrocyte implantation (ACI) or osteochondral grafting. PMID: 19660173 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2682636 Tue, 04 Aug 2009 23:00:00 +0100 2682636 http://www.medworm.com/index.php?rid=2682635&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19660174%26dopt%3DAbstract Authors: López-González R, Knuckles P, Velasco I Embryonic stem (ES) cells can be induced to differentiate into motor neurons (MN). Animal models resembling MN degeneration and paralysis observed in familial Amyotrophic Lateral Sclerosis (ALS) have been previously reported. In this work, we aimed to investigate whether transplanted MN could prevent motor deterioration in transgenic rats expressing a mutant form of human Superoxide dismutase 1 (hSOD1G93A) associated with inherited ALS. Mouse ES cells were differentiated to neurons that express Green fluorescent protein (GFP) under the promoter of the MN-specific gene hb9, as well as molecular markers indicative of MN identity. Cells were grafted into the lumbar spinal cord of adult wild-type (WT) or hSOD1G93A rats at 10 weeks ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2682635 Tue, 04 Aug 2009 23:00:00 +0100 2682635 http://www.medworm.com/index.php?rid=2682634&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19660175%26dopt%3DAbstract In conclusion, percutaneous echo-guided closed-chest CTx promises to be an effective and safe approach for CTx in small animal research. However, intramyocardial CTx of MNC or MSC had no influence on systolic function and contractile reserve after reperfused MI. PMID: 19660175 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2682634 Tue, 04 Aug 2009 23:00:00 +0100 2682634 http://www.medworm.com/index.php?rid=2682633&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19660176%26dopt%3DAbstract Authors: Castillo JE, D MD, García-Martín MC, D P, -Díaz JA, D MD, Giné E, D P, Vara E, D P, Cantero JE, Hon MD Our goal was to investigate whether previously related antiapoptotic and anti-inflammatory effects of tacrolimus could be useful in protecting human islets cultured in the presence of several pro-inflammatory mediators. Human islets obtained from cadaveric donors after intraductal infusion with collagenase, mechanical digestion and continuous Ficoll gradient purification were cultured in RPMI-1640 medium for 24 hours. Escherichia coli lipopolysaccharide (10mug/ mL) or Interleukin-1 (50 UI/ mL) plus gamma -IF (1000 UI/ mL) and low-dose tacroliumus (5 ng/ mL) were added. Homogenized samples (300 IE) from 5 different donors where assigned to 4 different exper... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2682633 Tue, 04 Aug 2009 23:00:00 +0100 2682633 http://www.medworm.com/index.php?rid=2682632&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19660177%26dopt%3DAbstract Authors: Cobacho N, Serrano AB, Casarejos MJ, Mena MA, Paíno CL Subarachnoidal grafting of monoamine-producing cells has been used with success to treat chronic pain in animal models. In the search for a source of autologous transplantable cells, capable of delivering neuroactive substances to the cerebrospinal fluid (CSF) to treat pain, we have tested adipose tissue stromal cells (ADSCs) transduced to produce levodopa. Intrathecally grafted ADSCs survive for long term adhered to spinal cord and nerve root meninges. Cultured ADSCs were retrovirally transduced with tyrosine hydroxylase (TH) and/or GTP cyclohydroxylase 1 (GCH1) genes and stably expressed them for at least 6 weeks in culture. Singly transduced cultures did not produce measurable levodopa but doubly transduced or a mi... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2682632 Tue, 04 Aug 2009 23:00:00 +0100 2682632 http://www.medworm.com/index.php?rid=2682631&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19660178%26dopt%3DAbstract In this study, we demonstrated the dynamic nature of epigenetic marks during the differentiation of quiescent adult rat NSCs in neurospheres. A subpopulation of OCT4+ NSCs in the neurosphere contained Histone marks, tri-methylated Histone 3 on lysine 27 (3me-H3K27), 2me-H3K4, and acetylated H4 (Ac-H4). A major decrease of these marks was found prior to or during differentiation, and was further diminished or reprogrammed in diverse subpopulations of migrated NSCs expressing nestin or beta-III-tubulin. The DNA methylation mark 5-methyl-cytosine (5-MeC) and the DNA methyltransferase (DNMT) 1 and 3a expression also correlated to the state of differentiation; they were highly present in undifferentiated NSCs but down-regulated in migrated populations. In contrast, the DNA methyl-CpG-binding pr... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2682631 Tue, 04 Aug 2009 23:00:00 +0100 2682631 http://www.medworm.com/index.php?rid=2682630&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19660179%26dopt%3DAbstract CURRENT STATUS OF STEM CELL THERAPY FOR LIVER DISEASES. Cell Transplant. 2009 Aug 5; Authors: de Freitas Souza BS, Nogueira RC, Andrade de S Liver failure is one of the main causes of death worldwide and is a growing healthproblem. Since the discovery of stem cell populations capable of differentiating intospecialized cell types, including hepatocytes, the possibility of their utilization in theregeneration of the damaged liver has been a focus of intense investigation. A variety ofcell types were tested both in vitro and in vivo, but the definition of a more suitable cellpreparation for therapeutic use in each type of liver lesions is yet to be determined. Here we review the protocols described for differentiation of stem cells into hepatocytes, the results of cell therapy in ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2682630 Tue, 04 Aug 2009 23:00:00 +0100 2682630 http://www.medworm.com/index.php?rid=2682629&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19660180%26dopt%3DAbstract Authors: A BC, R J, V M, A L, F C, Jv C, Mj GL Adipose tissue contains a mesenchymal stem cell (MSC) population known as adipose-derived stem cells (ASCs) capable of differentiating into different cell types. Our aim was to induce hepatic trans-differentiation of ASCs by sequential exposure to several combinations of cytokines, growth factors and hormones. The most efficient hepatogenic protocol includes fibroblastic growth factors (FGF) 2 and 4 and epidermal growth factor (EGF) (Step 1), hepatocyte growth factor (HGF), FGF2, FGF4 and nicotinamide (Nic) (Step 2), and oncostatin M (OSM), dexamethasone (Dex) and insulin-tranferrin-selenium (Step 3). This protocol activated transcription factors (GATA6, Hex, CCAAT/enhancer binding protein alpha and beta, CEBPalpha and beta, peroxisome pro... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2682629 Tue, 04 Aug 2009 23:00:00 +0100 2682629 http://www.medworm.com/index.php?rid=2682628&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19660181%26dopt%3DAbstract Authors: Thanos C, Emerich D, Bintz B, Goddard M, Mills J, Jensen R, Lombardi M The choroid plexus (CP), located at the blood-brain interface, is partially responsible for maintaining the composition of cerebrospinal fluid. Epithelial cell clusters isolated from the CP secrete numerous biologically active molecules, and are neuroprotective when transplanted in animal models of Huntington's disease and stroke. The genomic and proteomic profiles of CP may extend beyond CNS applications due to an abundance of trophic and regenerative factors including vascular endothelial growth factor, transforming growth factor beta, and others. Here, we used microarray to investigate the genomic profile of porcine CP epithelium, and then assessed the in vitro and in vivoregenerative capability of secre... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2682628 Tue, 04 Aug 2009 23:00:00 +0100 2682628 http://www.medworm.com/index.php?rid=2630501&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622226%26dopt%3DAbstract In this study, we cloned the cynomolgus homologue and showed that the ERas gene is expressed in cynomolgus ES cells. Notably, it is also expressed in cynomolgus ES cell-derived differentiated progeny as well as cynomolgus adult tissues. The ERas protein is detectable in various cynomolgus tissues as assessed by immunohistochemisty. Cynomolgus ES cell-derived teratoma cells, which also expressed the ERas gene at higher levels than the undifferentiated cynomolgus ES cells, did not develop tumors in NOD/Shi-scid, IL-2Rgamma(null) (NOG) mice. Even when the ERas gene was overexpressed in cynomolgus stromal cells, only the plating efficiency was improved and the proliferation was not promoted. Thus, it is unlikely that ERas contributes to the tumorigenicity of cynomolgus cells. Therefore, cynomo... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630501 Thu, 23 Jul 2009 13:12:03 +0100 2630501 http://www.medworm.com/index.php?rid=2630500&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622227%26dopt%3DAbstract Authors: Yang T, Tsang KS, Poon WS, Ng HK Embryonic stem (ES) cell-derived cell products may serve as a source of cells for regenerative medicine. Currently available technologies for the induction of ES cells into neural lineage cells require extended culturing in vitro and complex procedural manipulations, with variable yields of heterogeneous cells, which have hindered the prospective use of cell derivatives for treatment of ischemic stroke. We established a simple and efficient method to derive mouse ES cells into neural lineage cells using an 8-day coculture with the bone marrow stromal cells MS5, followed by a 6-day propagation culture and a 4-day selection culture. The protocol generated a relatively high yield of neural lineage cells without any mesodermal and endodermal lineag... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630500 Thu, 23 Jul 2009 13:12:03 +0100 2630500 http://www.medworm.com/index.php?rid=2630499&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622228%26dopt%3DAbstract Authors: Cargnoni A, Gibelli L, Tosini A, Signoroni PB, Nassuato C, Arienti D, Lombardi G, Albertini A, Wengler GS, Parolini O Fetal membranes (amnion and chorion) have recently raised significant attention as potential sources of stem cells. We have recently demonstrated that cells derived from human term placenta show stem cell phenotype, high plasticity, and display low immunogenicity both in vitro and in vivo. Moreover, placenta-derived cells, after xenotransplantation, are able to engraft in solid organs including the lung. On these bases, we studied the effects of fetal membrane-derived cells on a mouse model of bleomycin-induced lung fibrosis. Fetal membrane-derived cells were infused 15 min after intratracheal bleomycin instillation. Different delivery routes were used: intrape... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630499 Thu, 23 Jul 2009 13:12:03 +0100 2630499 http://www.medworm.com/index.php?rid=2630498&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622229%26dopt%3DAbstract Authors: Castanheira P, Torquetti LT, Magalhãs DR, Nehemy MB, Goes AM To evaluate DAPI (4',6-diamidino-2-phenylindole) as a nuclear tracer of stem cell migration and incorporation it was observed the pattern of retinal integration and differentiation of mesenchymal stem cells (MSCs) injected into the vitreous cavity of rat eyes with retinal injury. For this purpose adult rat retinas were submitted to laser damage followed by transplantation of DAPI-labeled BM-MSCs grafts and double-labeled DAPI and quantum dot-labeled BM-MSCs. To assess a possible DAPI diffusion as well as the integration and differentiation of DAPI-labeled BM-MSCs in laser-injured retina, host retinas were evaluated 8 weeks after injury/transplantation. It was demonstrated that, 8 weeks after the transplant, most... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630498 Thu, 23 Jul 2009 13:12:03 +0100 2630498 http://www.medworm.com/index.php?rid=2630497&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622230%26dopt%3DAbstract This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cultured to form a cell sheet. The MSC sheet was then wrapped onto structural allografts. The assembled structures were cultured in vitro to evaluate the differentiation potential of MSC sheet. The assembled structures were implanted subcutaneously into nude mice as well as into the segmental radius defect of rabbits to investigate the efficiency of MSC sheets to repopulate allografts for bone repair. MSC sheets, upon assembling on bone grafts, showed similar differentiation properties to the in situ periosteum in vitro. After implantation the MSC sheets accelerated the repopulation of bone grafts in nude mice. Moreover, MSC sheets induced thicker cortical bone for... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630497 Thu, 23 Jul 2009 13:12:03 +0100 2630497 http://www.medworm.com/index.php?rid=2630496&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622231%26dopt%3DAbstract Authors: Akiyama M, Nakamura M Reliable bone regeneration can be achieved with a pellet culture system using bovine periosteal cells. However, bone regeneration and neovascularization processes in this system have remained unclear. The present study aimed to clarify the extracellular environment and neovascularization process. To detect components of the extracellular matrix secreted by cells and to identify the conditions necessary for bone regeneration in the body, Western blotting and in vivo tests in nude mice were performed. Cells were cultured with or without ascorbic acid and culture supernatant was precipitated. Western blotting showed that culture supernatant contained collagen type I, procollagen type I, and procollagen type I C-terminus when cells were cultured with ascorbic... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630496 Thu, 23 Jul 2009 13:12:03 +0100 2630496 http://www.medworm.com/index.php?rid=2630495&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622232%26dopt%3DAbstract This study aimed to validate fructosamine use in islet transplant subjects and to evaluate its effectiveness as a predictor for islet graft dysfunction. Thirty-three islet transplant recipients who had concomitant fructosamine and HbA1c data available were retrospectively analyzed. HbA1c, fructosamine, mean capillary blood glucose, and islet graft function (fasting C-peptide/glucose ratio) were assessed. There was a significant and positive association between fructosamine and HbA1c (p < 0.0001). Both variables were also positively associated with mean overall and fasting capillary glucose. Neither fructosamine nor HbA1c was shown by ROC analysis to significantly discriminate between periods with and without subsequent graft dysfunction. HbA1c >6% was predictive of this outcome 1 mon... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630495 Thu, 23 Jul 2009 13:12:03 +0100 2630495 http://www.medworm.com/index.php?rid=2630494&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622233%26dopt%3DAbstract Authors: Bae JY, Kanamune J, Han DW, Matsumura K, Hyon SH We investigated the hibernation effect of epigallocatechin-3-O-gallate (EGCG) on neonatal human tarsal fibroblasts (nHTFs) by analyzing the expression of cell cycle-related genes. EGCG application to culture media moderately inhibited the growth of nHTFs, and the removal of EGCG from culture media led to complete recovery of cell growth. EGCG resulted in a slight decrease in the cell population of the S and G(2)/M phases of cell cycle with concomitant increase in that of the G(0)/G(1) phase, but this cell cycle profile was restored to the initial level after EGCG removal. The expression of cyclin D1 (CCND1), CCNE2, CCN-dependent kinase 6 (CDK6), and CDK2 was restored, whereas that of CCNA, CCNB1, and CDK1 was irreversibly attenu... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630494 Thu, 23 Jul 2009 13:12:03 +0100 2630494 http://www.medworm.com/index.php?rid=2630493&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622234%26dopt%3DAbstract Authors: Weyrauch UM, Torres EM, Baird AL, Dunnett SB Obtaining accurately staged rat embryos can be difficult because of the variety of breeding protocols employed and because precise staging cannot be confirmed until excision of the embryos from the dam. The detection of estrus, pairing of animals, and confirmation of pregnancies is generally left to commercial suppliers, as in-house breeding can be laborious and unpredictable. Here we describe a simple, reliable in-house breeding protocol for the generation of accurately staged embryos as assessed by measurements of average crown to rump length (CRL). PMID: 19622234 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630493 Thu, 23 Jul 2009 13:12:03 +0100 2630493 http://www.medworm.com/index.php?rid=2630492&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622235%26dopt%3DAbstract Authors: Fujimoto KL, Miki T, Liu LJ, Hashizume R, Strom SC, Wagner WR, Keller BB, Tobita K Stem cells contained in the amniotic membrane may be useful for cellular repair of the damaged heart. Previously, we showed that amnion-derived cells (ADCs) express embryonic stem cell surface markers and pluripotent stem cell-specific transcription factor genes. These ADCs also possess the potential for mesoderm (cardiac) lineage differentiation. In the present study we investigated whether untreated naive ADC transplantation into the injured left ventricular (LV) myocardium is beneficial as a cell-based cardiac repair strategy in a rat model. ADCs were isolated from Lewis rat embryonic day 14 amniotic membranes. FACS analysis revealed that freshly isolated ADCs contained stage-specific embryon... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630492 Thu, 23 Jul 2009 13:12:03 +0100 2630492 http://www.medworm.com/index.php?rid=2630491&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19622236%26dopt%3DAbstract Authors: PMID: 19622236 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2630491 Thu, 23 Jul 2009 13:12:03 +0100 2630491 http://www.medworm.com/index.php?rid=2557042&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558772%26dopt%3DAbstract Authors: Patel AN, Sherman W PMID: 19558772 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557042 Wed, 01 Jul 2009 10:14:03 +0100 2557042 http://www.medworm.com/index.php?rid=2557041&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558773%26dopt%3DAbstract Authors: Sanz-Ruiz R, Fernández-Santos E, Domínguez-Muñoa M, Parma R, Villa A, Fernández L, Sánchez PL, Fernández-Avilés F Over the past decade, cell therapy has emerged as a new approach to reversing myocardial ischemia. Several types of adult stem cells have been studied in both preclinical and clinical conditions for this purpose: bone marrow cells, circulating cells, and myoblasts. Nevertheless, the quest for the ideal "anti-ischemic" cell is still ongoing. Recently, the existence of a population of stem cells located in adipose tissue (adipose-derived stem cells) has been observed. These are able to differentiate into multiple cell lineages including cardiomyocytic differentiation. In this review we discuss the basic principles of adipose-derived... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557041 Wed, 01 Jul 2009 10:14:03 +0100 2557041 http://www.medworm.com/index.php?rid=2557040&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558774%26dopt%3DAbstract Authors: Alexander I, Edelman ER, Methe H Tissue engineering is a promising approach to implement endothelial cells as a cellular delivery therapy for vascular disease. We and others previously demonstrated that endothelial cells embedded in three-dimensional collagen-based matrices retain their full biosecretory spectrum, enabling them to serve as powerful regulators of vascular diseases. Fascinatingly, matrix embedding of endothelial cells not only allows for their implantation but also seems to provide protection from allo- and xenogeneic-triggered host immune responses. This is not an effect of simple physical shielding but a more fundamental influence of cell-matrix interconnectivity on the cellular immune phenotype. Reduced cytokine-induced levels of costimulatory and adhesion mo... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557040 Wed, 01 Jul 2009 10:14:03 +0100 2557040 http://www.medworm.com/index.php?rid=2557039&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558775%26dopt%3DAbstract Authors: Hess S, Methe H, Kim JO, Edelman ER Because changes in subendothelial matrix composition are associated with alterations of the endothelial immune phenotype, we sought to understand if cytokine-induced NF-kappaB activity and downstream effects depend on substrate adherence of endothelial cells (EC). We compared the upstream phosphorylation cascade, activation of NF-kappaB, and expression/secretion of downstream effects of EC grown on tissue culture polystyrene plates (TCPS) with EC embedded within collagen-based matrices (MEEC). Adhesion of natural killer (NK) cells was quantified in vitro and in vivo. NF-kappaB subunit p65 nuclear levels were significantly lower and p50 significantly higher in cytokine-stimulated MEEC than in EC-TCPS. Despite similar surface expression of TNF... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557039 Wed, 01 Jul 2009 10:14:03 +0100 2557039 http://www.medworm.com/index.php?rid=2557038&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558776%26dopt%3DAbstract In conclusion, cardiac patches generated in the peritoneum were developed into consistent tissue patches with properties to seal and correct myocardial defects. Our study also offers a viable rat model for screening and evaluating new concepts in cardiac reconstruction and engineering. PMID: 19558776 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557038 Wed, 01 Jul 2009 10:14:03 +0100 2557038 http://www.medworm.com/index.php?rid=2557037&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558777%26dopt%3DAbstract This study was designed to determine if a viable biodegradable three-dimensional fibroblast construct (3DFC) patch implanted on the left ventricle after myocardial infarction (MI) improves left ventricular (LV) function and blood flow. We ligated the left coronary artery of adult male Sprague-Dawley rats and implanted the 3DFC at the time of the infarct. Three weeks after MI, the 3DFC improved LV systolic function by increasing (p < 0.05) ejection fraction (37 +/- 3% to 62 +/- 5%), increasing regional systolic displacement of the infarcted wall (0.04 +/- 0.02 to 0.11 +/- 0.03 cm), and shifting the passive LV diastolic pressure volume relationship toward the pressure axis. The 3FDC improved LV remodeling by decreasing (p < 0.05) LV end-systolic and end-diastolic diameters with no chan... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557037 Wed, 01 Jul 2009 10:14:03 +0100 2557037 http://www.medworm.com/index.php?rid=2557036&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558778%26dopt%3DAbstract Authors: Martens TP, Godier AF, Parks JJ, Wan LQ, Koeckert MS, Eng GM, Hudson BI, Sherman W, Vunjak-Novakovic G Heart disease is the leading cause of death in the US. Following an acute myocardial infarction, a fibrous, noncontractile scar develops, and results in congestive heart failure in more than 500,000 patients in the US each year. Muscle regeneration and the induction of new vascular growth to treat ischemic disorders of the heart can have significant therapeutic implications. Early studies in patients with chronic ischemic systolic left ventricular dysfunction (SLVD) using skeletal myoblasts or bone marrow-derived cells report improvement in left ventricular ejection function (LVEF) and clinical status, without notable safety issues. Nonetheless, the efficacy of cell transfer ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557036 Wed, 01 Jul 2009 10:14:03 +0100 2557036 http://www.medworm.com/index.php?rid=2557035&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558779%26dopt%3DAbstract Authors: Das H, Abdulhameed N, Joseph M, Sakthivel R, Mao HQ, Pompili VJ The stem cell therapy for treating ischemic diseases is promising; however, the limited availability and compromised quality of progenitor cells in aged and diseased patients limit its therapeutic use. Here we report a nanofiber-based ex vivo stem cell expansion technology and proangiogenic growth factors overexpression of human umbilical cord blood (UCB)-derived progenitor cells to enhance angiogenic potential of therapeutic stem cells. The progenitor cells were expanded approximately 225-fold on nanofiber-based serum-free ex vivo expansion culture technique without inducing differentiation. The expanded cells express high levels of stem cell homing receptor, CXCR4, and adhesion molecule, LFA-1. The nanofiber-exp... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557035 Wed, 01 Jul 2009 10:14:03 +0100 2557035 http://www.medworm.com/index.php?rid=2557034&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558780%26dopt%3DAbstract We report rat MSCs isolated from young donors could undergo transformation in early passage culture. We aimed to characterize the transformed population and determine their therapeutic effects after intracardiac transplantation in the infarcted myocardium. MSCs were isolated from bone marrow of Lewis rats according to standard protocols and cultured under standard conditions. Phenotype of growing cells was assessed by flow cytometry. Following acute myocardial infarction in rats, cells were delivered by intracardiac injection. Cardiac functions were assessed by pressure-volume loops. Infarction size and pathologic effects were evaluated after 6 weeks. The abnormal colonies were detected in culture as early at passage 3. They were noted to appear as distinctly different morphology from typi... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557034 Wed, 01 Jul 2009 10:14:03 +0100 2557034 http://www.medworm.com/index.php?rid=2557033&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558781%26dopt%3DAbstract Authors: Sherman W, He KL, Yi GH, Wang J, Harvey J, Lee MJ, Haimes H, Lee P, Miranda E, Kanwal S, Burkhoff D Skeletal myoblast (SM) implantation promotes recovery of myocardial function after ischemic injury. Clinical observations suggest an association of SM implantation and ventricular arrhythmias. Support for this link has been sought in animal studies, but none employing models of congestive heart failure. In a canine model of postinfarction congestive heart failure (CHF) we compared the frequency of rhythm disturbances using ambulatory electrocardiography monitoring following skeletal myoblast or saline (SAL) implantation. In 19 mongrel dogs ischemic injury and CHF were induced by intracoronary microsphere infusions. Direct intramyocardial injection of autologous skeletal myoblast... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557033 Wed, 01 Jul 2009 10:14:03 +0100 2557033 http://www.medworm.com/index.php?rid=2557032&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558782%26dopt%3DAbstract The objective of this study was to investigate safety and feasibility of autologous bone marrow mononuclear cells (BMMNC) transplantation in ST elevation myocardial infarction (STEMI), comparing anterograde intracoronary artery (ICA) delivery with retrograde intracoronary vein (ICV) approach. An open labeled, randomized controlled trial of 30 patients admitted with STEMI was used. Patients were enrolled if they 1) were successfully reperfused within 24 h from symptoms onset and 2) had infarct size larger than 10% of the left ventricle (LV). One hundred million BMMNC were injected in the infarct-related artery (intra-arterial group) or vein (intravenous group), 1% of which was labeled with Tc(99m)-hexamethylpropylenamineoxime. Cell distribution was evaluated 4 and 24 h after injection. Base... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557032 Wed, 01 Jul 2009 10:14:03 +0100 2557032 http://www.medworm.com/index.php?rid=2557031&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558783%26dopt%3DAbstract Authors: Wehberg KE, Answini G, Wood D, Todd J, Julian J, Ogburn N, Allen KB Transmyocardial revascularization (TMR) can improve refractory angina but does not consistently demonstrate an effect on myocardial function. Recent studies suggest a synergistic effect between TMR and exogenously supplied growth factors. We evaluated the clinical role of intramyocardial injection of autologous platelet-rich plasma (PRP) in conjunction with TMR. Twenty-five nonrevascularizable patients with class III/IV angina underwent minimally invasive sole therapy TMR during a 5-year period at a single institution. Group 1 (14 patients) underwent TMR alone while group 2 (11 patients) underwent TMR plus injection of PRP (Magellan plasma separator) between TMR channels. Blinded angina assessment and ejection... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557031 Wed, 01 Jul 2009 10:14:03 +0100 2557031 http://www.medworm.com/index.php?rid=2557030&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19558784%26dopt%3DAbstract Authors: Nasseri BA, Kukucka M, Dandel M, Knosalla C, Choi YH, Ebell W, Hetzer R, Stamm C The subtle effects of transplanted bone marrow cells (BMC) on regional myocardial behavior in patients with ischemic heart disease are difficult to assess. Novel echocardiographic techniques can quantify regional myocardial deformation (strain) and distinguish between passive and active wall motion. We hypothesized that this technique may help delineate cell therapy-induced changes in regional LV contractility that escape clinical routine studies. Twelve patients with coronary artery disease and impaired LV function (LVEF &<35%) underwent CABG surgery plus intramyocardial injection of autologous bone marrow mononuclear cells. Between two and five predefined segments of ischemic myocardium p... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2557030 Wed, 01 Jul 2009 10:14:03 +0100 2557030 http://www.medworm.com/index.php?rid=2526354&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476204%26dopt%3DAbstract Authors: Stendahl JC, Kaufman DB, Stupp SI Intrahepatic islet transplantation provides a potentially more benign alternative to pancreatic transplantation. However, islet transplants are associated with limited engraftment potential. This inefficiency is likely at least partially attributable to the isolation process, which removes islets from their native environment. Isolation not only disrupts the internal vascularization and innervation of islets, but also fundamentally changes interactions between islet cells and macromolecules of the extracellular matrix (ECM). Signaling interactions between islet cells and ECM are known to regulate multiple aspects of islet physiology, including survival, proliferation, and insulin secretion. Although it is highly likely that disruptions to thes... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526354 Sat, 27 Jun 2009 11:30:04 +0100 2526354 http://www.medworm.com/index.php?rid=2526353&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476205%26dopt%3DAbstract In conclusion, RGP improved the efficiency in the purification of trapped islets, which often come from younger donor pancreata. The better quality of beta-cells in RGP islets encourages us to perform RGP, considering the higher quality as well as the quantity of remaining islets. PMID: 19476205 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526353 Sat, 27 Jun 2009 11:30:04 +0100 2526353 http://www.medworm.com/index.php?rid=2526352&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476206%26dopt%3DAbstract Authors: Lau J, Kampf C, Mattsson G, Nyqvist D, Köhler M, Berggren PO, Carlsson PO Pancreatic islets implanted heterotopically (i.e., into the kidney, spleen, or liver) become poorly revascularized following transplantation. We hypothesized that islets implanted into the pancreas would become better revascularized. Islets isolated from transgenic mice expressing enhanced yellow fluorescent protein (EYFP) in all somatic cells were cultured before they were implanted into the pancreas or beneath the renal capsule of athymic mice. Vascular density was evaluated in histological sections 1 month posttransplantation. EYFP was used as reporter for the transgene to identify the transplanted islets. Islet endothelial cells were visualized by staining with the lectin Bandeiraea simplicifoli... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526352 Sat, 27 Jun 2009 11:30:04 +0100 2526352 http://www.medworm.com/index.php?rid=2526351&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476207%26dopt%3DAbstract Authors: Kim HI, Yu JE, Lee SY, Sul AY, Jang MS, Rashid MA, Park SG, Kim SJ, Park CG, Kim JH, Park KS Instant blood-mediated inflammatory reaction (IBMIR) causes rapid islet loss in portal vein islet transplantation. Endothelial cells are known to protect against complement-mediated lysis and activation of coagulation. We tested composite pig islet-human endothelial cell grafts as a strategy to overcome IBMIR. Porcine islets were cocultured with human endothelial cells in specially modified culture medium composed of M199 and M200 for 1-9 days. A positive control group, negative control group, and the endothelial cell-coated group were examined with an in vitro tubing loop assay using human blood. The endothelial cell-coated group was subdivided and analyzed by degree of surface covera... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526351 Sat, 27 Jun 2009 11:30:04 +0100 2526351 http://www.medworm.com/index.php?rid=2526350&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476208%26dopt%3DAbstract In this study, we examined whether transplantation with bone marrow stromal cells (BMSCs) prevented tumor formation in SCI model mice that received ES cell-derived grafts containing both undifferentiated ES cells and neural stem cells. Embryoid bodies (EBs) formed in 4-day hanging drop cultures were treated with retinoic acid (RA) at a low concentration of 5 x 10(-9) M for 4 days, in order to allow some of the ES cells to remain in an undifferentiated state. RA-treated EBs were enzymatically digested into single cells and used as ES cell-derived graft cells. Mice transplanted with ES cell-derived graft cells alone developed tumors at the grafted site and behavioral improvement ceased after day 21. In contrast, no tumor development was observed in mice cotransplanted with BMSCs, which also ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526350 Sat, 27 Jun 2009 11:30:04 +0100 2526350 http://www.medworm.com/index.php?rid=2526349&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476209%26dopt%3DAbstract In this study, we first demonstrated the longevity, multipotency, and high fidelity of sensory features of postmigrating adult dorsal root ganglia (aDRG) stem cells. Derived from aDRG and after 4-5 years in culture without dissociating, the aDRG NSCs were found capable of proliferation, expressing neuroepithelial, neuronal, and glial markers. Remarkably, these aDRG NSCs expressed sensory neuronal markers vesicular glutamate transporter 2 (VGluT2--glutamate terminals), transient receptor potential vanilloid 1 (TrpV1--capsaicin sensitive), phosphorylated 200 kDa neurofilaments (pNF200--capsaicin insensitive, myelinated), and the serotonin transporter (5-HTT), which normally is transiently expressed in developing DRG. Furthermore, in response to neurotrophins, the aDRG NSCs enhanced TrpV1 exp... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526349 Sat, 27 Jun 2009 11:30:04 +0100 2526349 http://www.medworm.com/index.php?rid=2526348&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476210%26dopt%3DAbstract Authors: Koenig S, Krause P, Hosseini AS, Dullin C, Rave-Fraenk M, Kimmina S, Entwistle AL, Hermann RM, Hess CF, Becker H, Christiansen H Near infrared fluorescence (NIRF) optical imaging is a technique particularly powerful when studying in vivo processes at the molecular level in preclinical animal models. We recently demonstrated liver irradiation under the additional stimulus of partial hepatectomy as being an effective primer in the rat liver repopulation model based on hepatocyte transplantation. The purpose of this study was to assess optical imaging and the feasibility of donor cell expansion tracking in vivo using a fluorescent probe. Livers of dipeptidylpeptidase IV (DPPIV)-deficient rats were preconditioned with irradiation. Four days later, a partial hepatectomy was perform... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526348 Sat, 27 Jun 2009 11:30:04 +0100 2526348 http://www.medworm.com/index.php?rid=2526347&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476211%26dopt%3DAbstract Authors: Grondin M, Hamel F, Averill-Bates DA, Sarhan F Cryopreserved hepatocytes with good hepatospecific functions upon thawing are important for clinical transplantation and for in vitro drug toxicity testing. However, cryopreservation reduces viability and certain hepatospecific functions, but the most pronounced change is diminished attachment efficiency of hepatocytes. Adhesion of cells to the extracellular matrix and cell-cell contacts are crucial for many aspects of cellular function. These processes are partly mediated and controlled by cellular adhesion molecules. The mechanisms responsible for reduced attachment efficiency of cryopreserved hepatocytes are not well understood. To address this question, we investigated the effect of a new cryopreservation procedure, using whea... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526347 Sat, 27 Jun 2009 11:30:04 +0100 2526347 http://www.medworm.com/index.php?rid=2526346&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476212%26dopt%3DAbstract Authors: Hardjo M, Miyazaki M, Sakaguchi M, Masaka T, Ibrahim S, Kataoka K, Huh NH Transplantation of hepatocytes or bone marrow-derived cells has been shown to ameliorate liver fibrosis in animal models, but no direct comparison of relative efficiency has been made. The aim of this study was to compare the efficiency of a bone marrow-derived clonal mesenchymal stem cell line established by us (rBM25/S3) with that of its adipogenic or hepatogenic differentiation derivative for suppression of rat liver fibrosis. After induction of differentiation of rBM25/S3 cells into adipogenic or hepatogenic cells in culture, we intrasplenically transplanted the three types of cells into rats (3 x 10(7) cells/rat) before and 4 weeks after initiation of carbon tetrachloride treatment (1 ml/kg body wei... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526346 Sat, 27 Jun 2009 11:30:04 +0100 2526346 http://www.medworm.com/index.php?rid=2526345&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19476213%26dopt%3DAbstract Authors: Mei J, Sgroi A, Mai G, Baertschiger R, Gonelle-Gispert C, Serre-Beinier V, Morel P, Bühler LH The aim of this study was to establish hepatocyte isolation in pigs, and to evaluate function of isolated hepatocytes after encapsulation, cryopreservation, and transplantation (Tx) in a mouse model of fulminant liver failure (FLF). After isolation, porcine hepatocytes were microencapsulated with alginate-poly-L-Lysine-alginate membranes and cryopreserved. In vitro, albumin production of free and encapsulated hepatocytes were measured by enzyme linked-immunoadsorbent assay. In vivo, encapsulated hepatocytes were transplanted into different groups of mice with FLF and the following experimental groups were performed: group 1, Tx of empty capsules; group 2, Tx of free primary porci... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526345 Sat, 27 Jun 2009 11:30:04 +0100 2526345 http://www.medworm.com/index.php?rid=2526344&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19499700%26dopt%3DAbstract In this report, we review the current progress on the characterization of MSCs derived from the amnion as a remarkable transplantable cell population with therapeutic potential for multiple CNS disorders, especially stroke. PMID: 19499700 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526344 Sat, 27 Jun 2009 11:30:04 +0100 2526344 http://www.medworm.com/index.php?rid=2526343&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19499701%26dopt%3DAbstract This study has been performed in a rat 6-hydroxydopamine model of PD and is based on the microtransplantation approach. The results demonstrate a reduced survival (threefold) for a single cell suspension of E14 rat ventral mesencephalon compared to a fragment suspension when a metal cannula is used for implantation. However, fragment suspensions result in a more variable graft survival and ectopically placed cells along the implantation tract. When a glass capillary is used for implantation, the survival of the single cell suspension (so-called "micrograft") improved by fourfold (vs. single cells/metal cannula) and is superior to the combination of the metal cannula and fragment suspension (+40%). The micrografts show a reduced variability in DA neuron survival as well as fewer ectopically... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526343 Sat, 27 Jun 2009 11:30:04 +0100 2526343 http://www.medworm.com/index.php?rid=2526342&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19499702%26dopt%3DAbstract Authors: Kuleshova LL, Tan FC, Magalhães R, Gouk SS, Lee KH, Dawe GS Development of effective cryopreservation protocols will be essential to realizing the potential for clinical application of neural stem and progenitor cells. Current cryopreservation protocols have been largely employed in research, which does not require as stringent consideration of viability and sterility. Therefore, these protocols involve the use of serum and protein additives, which can potentially introduce contaminants, and slow cooling with DMSO/glycerol-based cryopreservation solutions, which impairs cell survival. We investigated whether serum- and protein-free vitrification is effective for functional cryopreservation of neurosphere cultures of neural stem or progenitor cells. To protect the samples ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526342 Sat, 27 Jun 2009 11:30:04 +0100 2526342 http://www.medworm.com/index.php?rid=2526341&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19499703%26dopt%3DAbstract Authors: Santiago LY, Clavijo-Alvarez J, Brayfield C, Rubin JP, Marra KG To test the hypothesis that the transplantation of adipose precursor cells (APCs) improves nerve regeneration and functional recovery, human APCs were transplanted into the lumen of a nerve guide in a 6-mm unilateral sciatic nerve defect in athymic rats. The three control groups for the study were biodegradable, polycaprolactone-based nerve conduit without APCs, autograft, and empty defect. Behavioral tests were performed every 3 weeks, and the sciatic functional index (SFI) was calculated based on measurements from the hindlimb prints. After 12 weeks, the nerve as well as right and left gastrocnemius muscles were removed and preserved for histological evaluation. Full regeneration of the sciatic nerve occurred on... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526341 Sat, 27 Jun 2009 11:30:04 +0100 2526341 http://www.medworm.com/index.php?rid=2526340&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19499704%26dopt%3DAbstract Authors: Wei Y, Hu H, Wang H, Wu Y, Deng L, Qi J Adipose-derived stem cells (ASCs) appear to be a useful stem cell population, which has been shown to possess multipotentiality. The aim of this study was to evaluate the utility of ASCs in tissue-engineered cartilage using a hybrid scaffold from fibrin-modified PLGA scaffold. ASCs were isolated from rabbit adipose tissue. The PLGA scaffold was prepared by low-temperature deposition technology and the hybrid scaffold was fabricated by a freeze-drying method. When ASCs were seeded onto fibrin-modified PLGA scaffold in vitro, enhanced cellular viability was observed compared to unmodified PLGA scaffold. The analysis of proteoglycan and collagen II revealed that fibrin-modified scaffold succeeded in inducing ASCs to differentiate into chond... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526340 Sat, 27 Jun 2009 11:30:04 +0100 2526340 http://www.medworm.com/index.php?rid=2526339&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19499705%26dopt%3DAbstract Authors: Mai XL, Ma ZL, Sun JH, Ju SH, Ma M, Teng GJ Magnetic resonance imaging (MRI) has proven to be effective in tracking the distribution of transplanted stem cells to target organs by way of labeling cells with superparamagnetic iron oxide particles (SPIO). However, the effect of SPIO upon labeled cells is still unclear on a cellular level. With this study, the proliferation and viability of New Zealand rabbit peripheral blood endothelial progenitor cells (EPCs) labeled with SPIO were evaluated and in vitro images were obtained using a 1.5 T MR scanner. Mononuclear cells (MNCs) were isolated from peripheral blood of the adult New Zealand rabbit and cultured in fibronectin-coated culture flasks, in which EPCs were identified from cell morphology, outgrowth characteristics, and inte... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526339 Sat, 27 Jun 2009 11:30:04 +0100 2526339 http://www.medworm.com/index.php?rid=2526337&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19499706%26dopt%3DAbstract Authors: Sumitran-Holgersson S, Nowak G, Thowfeequ S, Begum S, Joshi M, Jaksch M, Kjaeldgaard A, Jorns C, Ericzon BG, Tosh D Although the appearance of hepatic foci in the pancreas has been described in animal experiments and in human pathology, evidence for the conversion of human pancreatic cells to liver cells is still lacking. We therefore investigated the developmental plasticity between human embryonic pancreatic cells and liver cells. Cells were isolated and expanded from 7-8-week-old human fetal pancreata (HFP) and were characterized for the absence and presence of pancreatic and hepatic markers. In vitro expanded HFP were treated with fibroblast growth factor 2 (FGF2) and dexamethasone (DX) to induce a liver phenotye in the cells. These treated cells in various passages were f... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526337 Sat, 27 Jun 2009 11:30:04 +0100 2526337 http://www.medworm.com/index.php?rid=2526336&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19499707%26dopt%3DAbstract Authors: Cornolti R, Figliuzzi M, Remuzzi A Immunoisolation of pancreatic islets is extensively investigated for glycemic control in diabetic experimental animals. We previously reported that subcutaneous xenotransplantation of bovine islets protected by a selective polysulfone membrane successfully controlled glycemia in diabetic rats for up to 20 days. We then wondered whether immunoisolated islets have adequate oxygen supply in this device, where only diffusive transport allows cell function and survival. Here we set up an experimental technique to measure oxygen consumption rate (OCR) using a Clark's electrode inserted in a glass thermostated chamber connected to a data recorder and acquisition system. Bovine islets were isolated from 6-month-old calves, encapsulated in sodium algi... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526336 Sat, 27 Jun 2009 11:30:04 +0100 2526336 http://www.medworm.com/index.php?rid=2526335&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19499708%26dopt%3DAbstract Authors: Bertuzzi F, Cainarca S, Marzorati S, Bachi A, Antonioli B, Nano R, Verzaro R, Ricordi C The available information concerning the characteristics and composition of collagenase batches, which are effective in the digestion of human pancreas for islet transplants, is scarce and incomplete. A large inter- and intrabatched variability in activity and efficiency of blend enzymes available for isolation has been observed. The aim of this study was to characterize enzyme blend components. Liberase batches were characterized by SDS-PAGE analyses, microelectrophoresis, and then by MALDI-TOF MS analysis. Three main bands were detected by SDS-PAGE analysis and submitted to MALDI-TOF MS analysis. Two bands were found to correspond to class I (isoform beta and another of 106 kDa) and one t... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526335 Sat, 27 Jun 2009 11:30:04 +0100 2526335 http://www.medworm.com/index.php?rid=2526334&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19500466%26dopt%3DAbstract This study evaluates the effects of intramuscular bone marrow cell transplantation in patients with limb-threatening critical limb ischemia with a very high risk for major amputation. After failed or impossible operative and/or interventional revascularization and after unsuccessful maximum conservative therapy, 51 patients with impending major amputation due to severe critical limb ischemia had autologous bone marrow cells (BMC) transplanted into the ischemic leg. Patients 1-12 received Ficoll-isolated bone marrow mononuclear cells (total cell number 1.1 +/- 1.1 x 10(9)), patients 13-51 received point of care isolated bone marrow total nucleated cells (3.0 +/- 1.7 x 10(9)). Limb salvage was 59% at 6 months and 53% at last follow-up (mean 411 +/- 261 days, range 175-1186). Perfusion measur... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526334 Sat, 27 Jun 2009 11:30:04 +0100 2526334 http://www.medworm.com/index.php?rid=2526239&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523337%26dopt%3DAbstract CONCLUSIONS: Matrix embedding enables control of EC-substratum interaction. This in turn regulates chemokine and surface molecule expression and secretion, in particular of those compounds within NF-kappaB pathways, chemoattraction of NK-cells, local inflammation and tissue repair. PMID: 19523337 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526239 Mon, 18 May 2009 23:00:00 +0100 2526239 http://www.medworm.com/index.php?rid=2526233&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523339%26dopt%3DAbstract Authors: Patel AN, Sherman W Advances on multiple scientific fronts have brought insights into mechanisms of tissue healing and uncovered new potential to cellular recovery and replacement. With the unabated rise in the prevalence of cardiovascular disease, the interventional community is increasingly faced with caring for patients with chronic coronary, peripheral vascular, myocardial and conduction system diseases. The International Conference on Cell Therapy for Cardiovascular Diseases (IC3D) 2008 maintained its principal goal of integrating basic, translational and clinical data and manuscripts for this special issue of "Cell Transplantation" were submitted by its faculty. Several themes emerged from IC3D 2008. One in particular was the role played by tissue engineers in directing,... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526233 Mon, 18 May 2009 23:00:00 +0100 2526233 http://www.medworm.com/index.php?rid=2526231&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523340%26dopt%3DAbstract Authors: Lafreniere JF, Caron MC, Skuk D, Goulet M, Cheikh AR, Tremblay JP Duchenne Muscular Dystrophy (DMD) is an inherited disease and a main target of myogenic cell transplantation (MT). After the failure of the first clinical trials with DMD patients, the poor migration of transplanted cells has been suspected to be a major problem for a more effective clinical application of MT. Previous investigations suggested that the quantity and dispersion of myofibers containing donor cell nuclei might be improved by increasing the migration of the transplanted cells outside the injection sites. Since the co-injection of motogenic factors with human myoblasts enhanced their intra-muscular migration following MT in SCID mice, the present study aimed to investigate whether this approach was ap... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526231 Tue, 12 May 2009 23:00:00 +0100 2526231 http://www.medworm.com/index.php?rid=2526229&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523341%26dopt%3DAbstract Authors: Martens TP, Godier AF, Parks JJ, Wan LQ, Koeckert MS, Eng GM, Hudson BI, Sherman W, Vunjak-Novakovic G Heart disease is the leading cause of death in the U.S. Following an acute myocardial infarction, a fibrous, noncontractile scar develops, and results in congestive heart failure in more than 500,000 patients in the U.S. each year. Muscle regeneration and the induction of new vascular growth to treat ischemic disorders of the heart can have significant therapeutic implications. Early studies in patients with chronic ischemic SLVD using skeletal myoblasts or bone marrow-derived cells report improvement in left ventricular ejection function (LVEF) and clinical status, without notable safety issues. Nonetheless, the efficacy of cell-transfer for cardiovascular disease is not est... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526229 Tue, 12 May 2009 23:00:00 +0100 2526229 http://www.medworm.com/index.php?rid=2526227&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523342%26dopt%3DAbstract Authors: Cho DY, Lin SZ, Yang WK, Hsu DM, Lee HC, Lee WY, Liu SP Immunotherapy is a new light of hope for the treatment of malignant gliomas. The brain is no longer believed to be an immunologically privileged organ. The major advantage of immunotherapy is the tumor-specific cytotoxic effect on the tumor cells with minimal side effects. Autologous dendritic cells (DCs)-based immunotherapy is a promising and feasible method. DCs are the most potent antigen-presenting cells (APCs). DCs prime T lymphocytes by epitopic major histocompatibility (MHC) class I and II for CD8(+) cytotoxic T lymphocytes (CTLs) and CD4(+) T helper cells, respectively. From the tissue specimen examination after DCs-based immunotherapy, CD8(+) CTLs have replaced T regulatory cells (Tregs) as the major dominant tis... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526227 Tue, 12 May 2009 23:00:00 +0100 2526227 http://www.medworm.com/index.php?rid=2526225&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523343%26dopt%3DAbstract Authors: Young W Clinicians have long used Lithium to treat manic depression. They have also observed that lithium causes granulocytosis and lymphopenia while it enhances immunological activities of monocytes and lymphocytes. In fact, clinicians have long used lithium to treat granulocytopenia resulting from radiation and chemotherapy, to boost immunoglobulins after vaccination, and to enhance natural killer activity. Recent studies revealed a mechanism that ties together these disparate effects of lithium. Lithium acts through multiple pathways to inhibit glycogen synthetase kinase-3 beta (GSK3b). This enzyme phosphorylates and inhibits nuclear factors that turn on cell growth and protection programs, including the nuclear factor of activated t-cells (NFAT) and WNT/beta-catenin. In an... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526225 Tue, 12 May 2009 23:00:00 +0100 2526225 http://www.medworm.com/index.php?rid=2526223&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523344%26dopt%3DAbstract EARLY TRANSLATION OF ADIPOSE DERIVED CELL THERAPY FOR CARDIOVASCULAR DISEASE. Cell Transplant. 2009 May 6; Authors: Sanz-Ruiz R, Fernández-Santos E, Domínguez-Muñoa M, Parma R, Villa A, Fernández L, Sánchez PL, Fernández-Avilés F Over the past decade, cell therapy has emerged as a new approach to reversing myocardial ischemia. Several types of adult stem cells have been studied in both preclinical and clinical conditions for this purpose: bone marrow cells, circulating cells and myoblasts. Nevertheless, the quest for the ideal "anti-ischemic" cell is still ongoing. Recently, the existence of a population of stem cells located in adipose tissue (adipose-derived stem cells) has been observed. These are able to differentiate into multiple cell li... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526223 Tue, 05 May 2009 23:00:00 +0100 2526223 http://www.medworm.com/index.php?rid=2526221&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523345%26dopt%3DAbstract CONCLUSION: In this canine model of ischemic CHF, ASM implantation did not result in a significant increase in ventricular arrhythmias compared to controls animals. The potential for ASM implantation to affect time-domain parameters of HRV merits further study. PMID: 19523345 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526221 Tue, 05 May 2009 23:00:00 +0100 2526221 http://www.medworm.com/index.php?rid=2526219&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523346%26dopt%3DAbstract Authors: Koestenbauer S, Zisch A, Dohr G, Zech NH The reconstitution of adult stem cells may be a promising source for the regeneration of damaged tissues and for the reconstitution of organ dysfunction. However, there are two major limitations to the use of such cells: (1) they are rare, and (2) only a few types exist that can easily be isolated without harming the patient. The best studied and most widely used stem cells are of the hematopoietic lineage. Pioneering work on hematopoietic stem cell (HSC) transplantation was done in the early 1970s by ED. Thomas and colleagues. Since then HSCs have been used in allogenic and autologous transplantation settings to reconstitute blood formation after high-dose chemotherapy for various blood disorders. The cells can be easily harvested from... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526219 Tue, 05 May 2009 23:00:00 +0100 2526219 http://www.medworm.com/index.php?rid=2526218&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523347%26dopt%3DAbstract In conclusion the incidence of bacterial contamination of the final product was low confirming both the suitability of the organs used, hepatocyte isolation procedure and the environmental conditions of the clean room. PMID: 19523347 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526218 Tue, 05 May 2009 23:00:00 +0100 2526218 http://www.medworm.com/index.php?rid=2526216&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523348%26dopt%3DAbstract In this study, we tested the hypothesis that the therapeutic capacity of MDSCs transplanted into myocardium is influenced by sex of donor MDSCs or recipient. Male and female MDSCs isolated from the skeletal muscle of 3 week old mice were transplanted into recipient male or female dystrophin-deficient (mdx) hearts or into the hearts of male SCID mice following acute myocardial infarction. In the mdx model, no difference was seen in engraftment or blood vessel formation based on donor cell or recipient sex. In the infarction model, MDSC transplanted hearts showed higher post-infarction angiogenesis, less myocardial scar formation, and improved cardiac function compared to vehicle controls. However, sex of donor MDSCs had no significant effects on engraftment, angiogenesis, and cardiac functi... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526216 Tue, 05 May 2009 23:00:00 +0100 2526216 http://www.medworm.com/index.php?rid=2526267&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523325%26dopt%3DAbstract In conclusion, autologous matrix assisted MSC transplantation significantly increased the histomorphological repair tissue quality during early articular cartilage defect repair and resulted in higher GAG/collagen type II positive cross sectional areas of the regenerated tissue. PMID: 19523325 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526267 Tue, 28 Apr 2009 23:00:00 +0100 2526267 http://www.medworm.com/index.php?rid=2526264&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523326%26dopt%3DAbstract Authors: Weyrauch UM, Torres EM, Baird AL, Dunnett SB Obtaining accurately staged rat embryos can be difficult because of the variety of breeding protocols employed and because precise staging cannot be confirmed until excision of the embryos from the dam. The detection of oestrus, pairing of animals and confirmation of pregnancies is generally left to commercial suppliers, as in house breeding can be laborious and unpredictable. Here we describe a simple, reliable in-house breeding protocol for the generation of accurately-staged embryos as assessed by measurements of average crown to rump length (CRL). PMID: 19523326 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526264 Tue, 28 Apr 2009 23:00:00 +0100 2526264 http://www.medworm.com/index.php?rid=2526261&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523327%26dopt%3DAbstract Authors: Epple-Farmer J, Debeb BG, Smithies O, Binas B In view of the well-known phenomenon of trophoblast immune privilege, trophoblast stem cells (TSCs) might be expected to be immune-privileged, which could be of interest for cell or gene therapies. Yet in the ectopic sites tested so far, TSC transplants fail to show noticeable immune privilege and seem to lack physiological support. However, we show here that after portal venous injection, green fluorescent protein (GFP)-labeled TSCs survive for several months in the livers of allogeneic female but not male mice. Gonadectomy experiments revealed that this survival does not require the presence of ovarian hormones but does require the absence of testicular factors. By contrast, GFPlabeled allogeneic embryonic stem cells (ESCs) are r... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526261 Tue, 28 Apr 2009 23:00:00 +0100 2526261 http://www.medworm.com/index.php?rid=2526258&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523328%26dopt%3DAbstract Authors: Kuo TK, Ho JH, Lee OK Mesenchymal stem cells are stem/progenitor cells originated from the mesoderm and can different into multiple cell types of the musculoskeletal system. The vast differentiation potential and the relative ease for culture-expansion have established mesenchymal stem cells as the building blocks in cell therapy and tissue engineering applications for a variety of musculoskeletal diseases including repair of fractures and bone defects, cartilage regeneration, treatment of osteonecrosis of the femoral head, and correction of genetic diseases such as osteogenesis imperfect. However, research in the past decade has revealed differentiation potentials of mesenchymal stem cells beyond lineages of the mesoderm, suggesting broader applications than originally percei... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526258 Tue, 28 Apr 2009 23:00:00 +0100 2526258 http://www.medworm.com/index.php?rid=2526256&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523329%26dopt%3DAbstract In conclusion, cardiac patches generated in the peritoneum were developed into consistent tissue patches with properties to seal and correct myocardial defects. Our study also offers a viable rat model for screening and evaluating new concepts in cardiac reconstruction and engineering. PMID: 19523329 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526256 Tue, 28 Apr 2009 23:00:00 +0100 2526256 http://www.medworm.com/index.php?rid=2526254&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523330%26dopt%3DAbstract In this study, we focused on the microRNA regulation in multiple stem cells. For example, miR-520h was up-regulated and miR-129 was down-regulated in HSC. MiR-103, 107, 140, 143, 638, 663 were associated with MSCs while miR-302s and miR-136 were associated with ESCs. In NSCs, miR-92b, let-7 and miR-125 were the critical regulators. The overview of the recent advances in the aspects of molecular control of stem cell biology reveals the important of microRNAs, and much helpful for future work. PMID: 19523330 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526254 Tue, 28 Apr 2009 23:00:00 +0100 2526254 http://www.medworm.com/index.php?rid=2526252&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523331%26dopt%3DAbstract Authors: Yamashita T, Deguchi K, Nagotani S, Kamiya T, Abe K Possible strategies for treating ischemic stroke include: 1) Neuroprotection: preventing injured neurons from undergoing apoptosis in the acute phase of cerebral ischemia; 2) Stem cell therapy: the repair of disrupted neuronal networks with newly born neurons in the chronic phase of cerebral ischemia. Firstly, we estimated the neuroprotective effect of glial cell line-derived neurotrophic factor (GDNF) by administration of GFNF protein. GDNF protein showed a direct protective effect against ischemic brain damage. Pretreatment of animals with adenoviral vector containing GDNF gene (Ad-GDNF) 24 hours before the subsequent transient middle cerebral artery occlusion (MCAO) effectively reduced infarcted volume. Secondly, we studie... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526252 Tue, 28 Apr 2009 23:00:00 +0100 2526252 http://www.medworm.com/index.php?rid=2526249&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523332%26dopt%3DAbstract Authors: Fu RH, Liu SP, Ou CW, Yu HH, Li KW, Tsai CH, Shyu WC, Lin SZ Stem cells have the surprising potential to develop into many different cell types. Therefore, major research efforts have focused on transplantation of stem cells and/or derived progenitors for restoring depleted diseased cells in degenerative disorders. Understanding the molecular controls include alternative splicing that arise during lineage differentiation of stem cells is crucial for developing stem cell therapeutic approaches in regeneration medicine. Alternative splicing allowing single gene to encode multiple transcripts with different protein coding sequences and RNA regulatory elements increases genomic complexities. Utilizing differences in alternative splicing as a molecular marker may be more sensitive ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526249 Tue, 28 Apr 2009 23:00:00 +0100 2526249 http://www.medworm.com/index.php?rid=2526247&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523333%26dopt%3DAbstract Authors: Park DH, Borlongan CV, Willing AE, Eve DJ, Sanberg PR Irreversible and permanent damage develop immediately adjacent to the region of reduced cerebral blood perfusion in stroke patients. Currently, the proven thrombolytic treatment for stroke, tissue-plasminogen activator, is only effective when administered within 3 hours after stroke. These disease characteristics should be taken under consideration in developing any therapeutic intervention designed to widen the narrow therapeutic range, especially cell-based therapy. Over the past several years, our group and others have characterized the therapeutic potential of human umbilical cord blood cells for stroke and other neurological disorders using in vitro and vivo models focusing on the cells' ability to differentiate into n... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526247 Tue, 28 Apr 2009 23:00:00 +0100 2526247 http://www.medworm.com/index.php?rid=2526245&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523334%26dopt%3DAbstract In this study, we demonstrate that AMTC can block differentiation and maturation of monocytes into dendritic cells (DC), preventing the expression of the DC marker CD1a and reducing the expression of HLA-DR, CD80 and CD83. The monocyte maturation block resulted in impaired allostimulatory ability of these cells on allogeneic T cells. In attempting to define the mechanisms responsible for these findings, we have observed that the presence of AMTC in differentiating DC cultures results in the arrest of the cells to the G0 phase and abolishes the production of inflammatory cytokines such as TNF-alpha, CXCL10, CXCL9 and CCL5. Finally, we also demonstrate that the monocytic cells present in the amniotic mesenchymal region fail to differentiate toward the DC lineage. Taken together, our data sug... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526245 Tue, 28 Apr 2009 23:00:00 +0100 2526245 http://www.medworm.com/index.php?rid=2526241&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523336%26dopt%3DAbstract Authors: Alexander I, Edelman ER, Methe H Tissue engineering is a promising approach to implement endothelial cells as a cellular delivery therapy for vascular disease. Others and we previously demonstrated that endothelial cells embedded in three-dimensional collagen-based matrices retain their full biosecretory spectrum enabling them to serve as powerful regulators of vascular diseases. Fascinatingly, matrix embedding of endothelial cells not only allows for their implantation but also seems to provide protection from allo- and xenogeneic triggered host immune responses. This is not an effect of simple physical shielding but a more fundamental influence of cell-matrix interconnectivity on the cellular immune phenotype. Reduced cytokine-induced levels of costimulatory and adhesion mol... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526241 Tue, 28 Apr 2009 23:00:00 +0100 2526241 http://www.medworm.com/index.php?rid=2526237&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19523338%26dopt%3DAbstract CONCLUSION: Injection procedures through anterograde and retrograde approaches seem to be feasible and safe. BMMNC retention by damaged heart tissue was apparently higher when the anterograde approach was used. Further studies are required to confirm these initial data. PMID: 19523338 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526237 Tue, 28 Apr 2009 23:00:00 +0100 2526237 http://www.medworm.com/index.php?rid=2526283&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19520045%26dopt%3DAbstract Authors: Iken M, Brandhorst H, Korsgren O, Brandhorst D Successful pancreas preservation during storage in oxygenated perfluorodecalin (PFD) is mainly related to oxidative ATP generation during storage. Increasing the storage temperature would accelerate this process essential for resuscitation of ischemically damaged pancreatic tissue. The present study aimed at comparing islet isolation outcome from adult pig pancreata preserved in oxygenated PFD by means of a one-layer method during storage on ice or at 20 degrees C. Resected pancreata were intraductally flushed with cold UW-solution and promptly processed (n = 6) or stored for three hours in continuously oxygenated PFD at 4 degrees C (n = 5) or 20 degrees C (n = 7). Prior to digestion-filtration pancreata were intraductally injecte... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526283 Wed, 22 Apr 2009 23:00:00 +0100 2526283 http://www.medworm.com/index.php?rid=2526281&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19520046%26dopt%3DAbstract CONCLUSIONS: Treatment with PDGF-BB seems to prevent loss of transplanted bone marrow cells at later times presumably by inhibition of apoptosis, while reperfusion of the occluded artery enhances cell loss at early times putatively due to enhanced early wash-out. Further investigations are needed to substantially improve the persistence and survival of grafted bone marrow cells in infarcted rat hearts, in order to fully explore the therapeutic potential of this novel treatment modality for myocardial repair. PMID: 19520046 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526281 Wed, 22 Apr 2009 23:00:00 +0100 2526281 http://www.medworm.com/index.php?rid=2526279&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19520047%26dopt%3DAbstract Authors: Benabdallah BF, Bouchentouf M, Rousseau J, Tremblay JP Duchenne muscular dystrophy is caused by the absence of functional dystrophin, leading to the myofiber membrane instability and progressive muscle atrophy. Myoblast transplantation in dystrophic muscles is a potential therapy, as it permits the long term restoration of dystrophin expression in transplanted muscles. However, the success of this approach is limited by the short period of muscle repair following myoblast transplantation. Myostatin, a powerful inhibitor of muscle growth, is involved in terminating the period of muscle repair following injury by reducing myoblast proliferation and differentiation. Follistatin forms a complex with myostatin preventing its interaction with its receptor and thus blocking the myost... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526279 Wed, 22 Apr 2009 23:00:00 +0100 2526279 http://www.medworm.com/index.php?rid=2526324&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19500468%26dopt%3DAbstract Authors: Daadi MM, Lee SH, Arac A, Grueter BA, Bhatnagar R, Maag AL, Schaar B, Malenka RC, Palmer TD, Steinberg GK Currently there are no effective treatments targeting residual anatomical and behavioral deficits resulting from stroke. Evidence suggests that cell transplantation therapy may enhance functional recovery after stroke through multiple mechanisms. We used a syngeneic model of neural transplantation to explore graft-host communications that enhance cellular engraftment. The medial ganglionic eminence (MGE) cells were derived from 15-day old transgenic rat embryos carrying the green fluorescent protein (GFP), a marker, to easily track the transplanted cells. Adult rats were subjected to transient intraluminal occlusion of the medial cerebral artery. Two weeks after stroke, th... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526324 Tue, 14 Apr 2009 23:00:00 +0100 2526324 http://www.medworm.com/index.php?rid=2526277&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19520048%26dopt%3DAbstract Authors: Akiyama M, Nakamura M Reliable bone regeneration can be achieved with a pellet culture system using bovine periosteal cells. However, bone regeneration and neovascularization processes in this system have remained unclear. The present study aimed to clarify the extracellular environment and neovascularization process. To detect components of the extracellular matrix secreted by cells and to identify the conditions necessary for bone regeneration in the body, Western blotting and in vivo tests in nude mice were performed. Cells were cultured with or without ascorbic acid and culture supernatant was precipitated. Western blotting showed that culture supernatant contained collagen type I, procollagen type I and procollagen type I C-terminus when cells were cultured with ascorbic ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526277 Tue, 14 Apr 2009 23:00:00 +0100 2526277 http://www.medworm.com/index.php?rid=2526275&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19520049%26dopt%3DAbstract Authors: Yang T, Tsang KS, Poon WS, Ng HK Embryonic stem (ES) cell-derived cell products may serve as a source of cells for regenerative medicine. Currently available technologies for the induction of ES cells into neural lineage cells require extended culturing in vitro and complex procedural manipulations, with variable yields of heterogeneous cells, which have hindered the prospective use of cell derivatives for treatment of ischemic stroke. We established a simple and efficient method to derive mouse ES cells into neural lineage cells using an 8-day co-culture with the bone marrow stromal cells MS5, followed by a 6-day propagation culture and a 4-day selection culture. The protocol generated a relatively high yield of neural lineage cells without any mesodermal and endodermal linea... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526275 Tue, 14 Apr 2009 23:00:00 +0100 2526275 http://www.medworm.com/index.php?rid=2526273&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19520050%26dopt%3DAbstract In this study, we cloned the cynomolgus homologue and showed that the ERas gene is expressed in cynomolgus ES cells. Notably, it is also expressed in cynomolgus ES cell-derived differentiated progeny as well as cynomolgus adult tissues. The ERas protein is detectable in various cynomolgus tissues as assessed by immunohistochemisty. Cynomolgus ES cell-derived teratoma cells, which also expressed the ERas gene at higher levels than the undifferentiated cynomolgus ES cells, did not develop tumors in NOD/Shi-scid, IL-2Rgamma(null) (NOG) mice. Even when the ERas gene was overexpressed in cynomolgus stromal cells, only the plating efficiency was improved and the proliferation was not promoted. Thus, it is unlikely that ERas contributes to the tumorigenecity of cynomolgus cells. Therefore, cynomo... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=2526273 Tue, 14 Apr 2009 23:00:00 +0100 2526273