MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Cell Transplantation' source. Tue, 07 Feb 2012 08:48:55 +0100 http://www.medworm.com/index.php?rid=5602085&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22236446%26dopt%3DAbstract We examined wounds in the CXCR3-/- mouse scarring model. These wounds exhibited a significant delay in healing in all areas compared to young and aged wild-type mice. Full-thickness wounds were transplanted with fibroblasts derived from newborn CXCR3-/- or wild-type mice. The transplanted fibroblasts were labeled with fluorescent dye (CM-DiI) and suspended in hyaluronic-acid gel; by 30 days, these transplanted cells comprised some 30% of the dermal stromal cells regardless of the host or source of transplanted cells. Wild-type fibroblasts transplanted into CXCR3-/-mice wounds reversed the delay and dysfunction previously seen in CXCR3-/-wounds; this correction was not noted with transplanted CXCR3-/-fibroblasts. Additionally, transplant of CXCR3-/- cells into wounds in wild-type animals di... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5602085 Tue, 10 Jan 2012 05:00:00 +0100 5602085 http://www.medworm.com/index.php?rid=5602084&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22236577%26dopt%3DAbstract This study aimed to investigate whether recombinant human bone morphogenetic protein-2 (rhBMP-2)-loaded calcium-sulfate (CS) combined with mesenchymal stem cell (MSC) sheets could accelerate bone regeneration in ulnar segmental defects of rabbits. In vitro, the osteogenic differentiation of MSCs cultured on rhBMP-2-loaded CS was investigated. Forty complete 1.2-cm bone defects were treated with CS (group A), rhBMP-2-loaded CS (group B), MSC sheets wrapped CS (group C) and MSC sheets wrapped rhBMP-2-loaded-CS (group D). After 4 and 8 weeks after implantation, the samples were treated by X-ray, micro-computed tomography and histological observation. The rhBMP-2 could be released from the rhBMP-2-loaded-CS scaffolds and maintain its bioactivity. The alkaline phosphatase (ALP) of MSCs cultured... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5602084 Tue, 10 Jan 2012 05:00:00 +0100 5602084 http://www.medworm.com/index.php?rid=5602083&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22236637%26dopt%3DAbstract In this study, we explored the possibility of achieving functional recovery of the urinary sphincter muscle using autologous muscle precursor cells (MPCs) as an injectable, cell-based therapy. A canine model of striated urinary sphincter insufficiency was created by microsurgically removing part of the sphincter muscle in 24 dogs. Autologous MPCs were obtained, expanded in culture and injected into the damaged sphincter muscles of 12 animals. The animals were followed for up to 6 months after injection, and urodynamic studies, functional organ bath studies, ultrastructural and histological examinations were performed. Animals receiving MPC injections demonstrated sphincter pressures of approximately 80% of normal values, while the pressures in the control animals without cells dropped and ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5602083 Tue, 10 Jan 2012 05:00:00 +0100 5602083 http://www.medworm.com/index.php?rid=5602082&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22236690%26dopt%3DAbstract Authors: Iglesias I, Valiente L, Shiang KD, Ichii H, Kandeel F, Al-Abdullah IH Abstract The choice of enzyme blend is critical for successful islet isolation. Islet yield, viability, integrity, and function are important factors that influence the outcome of islet transplantation. Liberase HI has been used as a standard enzyme for pancreas digestion and has successfully produced islets that reversed diabetes. However, the replacement of Liberase HI with collagenase NB1 has significantly influenced the process outcome, both in quality and quantity of the isolated islets. The assessment of islet cells by Flow Cytometry (FC) has been reported to be useful for evaluating islet quality. The aim of this study was to assess the isolation outcomes and islet quality when comparing human isl... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5602082 Tue, 10 Jan 2012 05:00:00 +0100 5602082 http://www.medworm.com/index.php?rid=5602081&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22236767%26dopt%3DAbstract In conclusion, we showed that growth factors increased survival of transplanted NSPCs whereas NgR enhanced axonal regeneration, but the combination did not have additive effects on functional recovery or regeneration. PMID: 22236767 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5602081 Tue, 10 Jan 2012 05:00:00 +0100 5602081 http://www.medworm.com/index.php?rid=5550329&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22195604%26dopt%3DAbstract Authors: Prabakar KR, Domínguez-Bendala J, Molano RD, Pileggi A, Villate S, Ricordi C, Inverardi L Abstract We sought to assess the potential of human cord blood-derived mesenchymal stem cells (CB-MSCs) to derive insulin-producing, glucose-responsive cells. We show here that differentiation protocols based on step-wise culture conditions initially described for human embryonic stem cells (hESCs) lead to differentiation of cord blood-derived precursors towards a pancreatic endocrine phenotype, as assessed by marker expression and in vitro glucose-regulated insulin secretion. Transplantation of these cells in immune-deficient animals shows human c-peptide production in response to a glucose challenge. This data suggests that human cord blood may be a promising source for regenerativ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5550329 Wed, 21 Dec 2011 05:00:00 +0100 5550329 http://www.medworm.com/index.php?rid=5535119&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22186137%26dopt%3DAbstract Authors: Nieto M, Hevia P, Garcia E, Klein D, Alvarez-Cubela S, Bravo-Egana V, Rosero S, Molano RD, Vargas N, Ricordi C, Pileggi A, Diez J, Domínguez-Bendala J, Pastori RL Abstract MicroRNAs regulate gene expression by inhibiting translation or inducing target mRNA degradation. MicroRNAs regulate organ differentiation and embryonic development, including pancreatic specification and islet function. We showed previously that miR-7 is highly expressed in human pancreatic fetal and adult endocrine cells. Here we determined the expression profile of miR-7 in the mouse-developing pancreas by RT-PCR and in situ hybridization. MiR-7 expression was low between embryonic day e10.5 and e11.5, then began to increase at e13.5 through e14.5 and eventually decreased by e18. In situ hybridizatio... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5535119 Tue, 20 Dec 2011 05:00:00 +0100 5535119 http://www.medworm.com/index.php?rid=5535120&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22183042%26dopt%3DAbstract In this study, intra-arterial autologous bone-marrow mononuclear cells were infused in patients with moderate to severe acute middle-cerebral-artery infarcts. The subjects of this study included 20 patients with early or late spontaneous recanalization but with persistent deficits, in whom treatment could be initiated between 3 and 7 days after stroke onset. Mononuclear cells were isolated from bone-marrow aspirates and infused at the proximal middle cerebral artery of the affected hemisphere. Safety analysis (primary endpoint) during the 6-month follow-up assessed death, any serious clinical events, neurological worsening with ≥4-point increase in National Institutes of Health Stroke Scale (NIHSS) scores, seizures, epileptogenic activity on electroencephalogram, and neuroimaging complic... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5535120 Wed, 14 Dec 2011 05:00:00 +0100 5535120 http://www.medworm.com/index.php?rid=5535124&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22182695%26dopt%3DAbstract Authors: Fagerlund M, Estrada CP, Jaff N, Svensson M, Brundin L Abstract Transplantation of neural stem cells and the mobilization of endogenous neuronal precursors in the adult brain have been proposed as therapeutic strategies for central nervous system disorders and injuries. The aim of the present study was to investigate the possible survival and integration of grafted neural progenitor cells (NPCs) from the subventricular zone (SVZ) in a hypoglossal nerve avulsion model with substantial neuronal loss. Adult neural progenitor cells (NPCs) from the subventricular zone (SVZ) were cultured from inbred transgenic eGFP Lewis rats and transplanted to the hypoglossal nucleus of inbred Lewis rat from the same family but that were not carrying the eGFP strain after avulsion of the hypo... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5535124 Tue, 13 Dec 2011 05:00:00 +0100 5535124 http://www.medworm.com/index.php?rid=5535123&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22182941%26dopt%3DAbstract Conclusions: ER stress mediators were induced during islet isolation and may contribute to islet apoptosis and cell death. Islet isolation activates ER stress and apoptotic pathways in isolated islets. Hyperglycemia may prolong this ER stress signal in engrafted islets, converting the protective aspects of the ER stress response to a proapoptotic response and thus contribute to deterioration of β-cell function and survival. PMID: 22182941 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5535123 Tue, 13 Dec 2011 05:00:00 +0100 5535123 http://www.medworm.com/index.php?rid=5535122&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22182968%26dopt%3DAbstract Authors: Boltze J, Schmidt UR, Reich DM, Kranz A, Reymann KG, Strassburger M, Lobsien D, Wagner DC, Förschler A, Schäbitz WR Abstract Experimental treatment strategies using human umbilical cord blood mononuclear cells (hUCB MNCs) represent a promising option for alternative stroke therapies. An important point for clinical translation of such treatment approaches is knowledge on the therapeutic time window. Although expected to be wider than for thrombolysis, the exact time window for hUCB MNC therapy is not known. Our study aimed to determine the time window of intravenous hUCB MNC administration after middle cerebral artery occlusion (MCAO). Male spontaneously hypertensive rats underwent MCAO and were randomly assigned to hUCB MNC administration at 4h, 24h, 72h, 120h or 14d. I... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5535122 Tue, 13 Dec 2011 05:00:00 +0100 5535122 http://www.medworm.com/index.php?rid=5535121&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22182982%26dopt%3DAbstract This study was to compare human four blood groups cord blood serum (CBS) with FBS in culturing human placenta-derived mesenchymal stem cells (hPDMSCs), which were derived from four blood groups donors. The expansion medium included: 10%FBS, 10% A-CBS, 10%B-CBS, 10%O-CBS and 10% AB-CBS. Cumulative population doubling, generation time, fold expansion rates and differentiation capacity, cell cycle and immunophenotype were also assessed. The result showed that fold expansion rate and cumulative population doubling of hPDMSCs significantly increased during long term MSCs expansion in CBS medium, but the generation time decreased compared with FBS. CBS might be an effective supplement for stem cells expand rapidly ex vivo. Cell cycle and differentiation assays showed that most of hPDMSCs expandi... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5535121 Tue, 13 Dec 2011 05:00:00 +0100 5535121 http://www.medworm.com/index.php?rid=5421163&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22080749%26dopt%3DAbstract Authors: Sanberg PR, Vindrola-Padros C, Eve DJ, Federoff HJ Abstract The following commentary provides a discussion of the articles published in Technology and Innovation in 2010 and where possible places them into context with those reported in Cell Transplantation. These articles can be divided into the following topics: a) models for innovation and technological commercialization, b) the ethical and legal consequences of the emergence of new technologies, c) research on novel technologies and methods, and d) the difficulties involved in peer review and scientific assessment. The articles shed light on the effects of technological innovation and commercialization on scientific ethical regulation, the establishment of legal standards for the protection of intellectual property, an... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5421163 Fri, 18 Nov 2011 23:18:02 +0100 5421163 http://www.medworm.com/index.php?rid=5421165&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22080676%26dopt%3DAbstract Authors: Alfaro MP, Young PP Abstract The regenerative and reparative potential of Mesenchymal Stem Cells (MSCs) make them attractive candidates for numerous cell-directed therapies. The variant degree of tissue repair by transplanted MSCs has been assessed in several published reports. There are many gaps in the knowledge of MSC biology and the underlying reasons for their disparate effectiveness in tissue repair. This review examines successful pre-clinical models of MSC-directed repair, particularly of myocardial repair, in an attempt to shed light into the events dictating MSC therapeutic efficacy. The reparative advantage of genetically altered MSCs will be described. This overview will elucidate possible molecular mechanisms that can influence MSC engraftment, differentiation... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5421165 Fri, 11 Nov 2011 05:00:00 +0100 5421165 http://www.medworm.com/index.php?rid=5421164&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22080748%26dopt%3DAbstract Conclusion: Magnetic targeting has the potential to enhance myocardial retention of intravascularly delivered endothelial progenitor cells. PMID: 22080748 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5421164 Fri, 11 Nov 2011 05:00:00 +0100 5421164 http://www.medworm.com/index.php?rid=5421162&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22080793%26dopt%3DAbstract Authors: Gramignoli R, Green ML, Tahan V, Dorko K, Skvorak KJ, Marongiu F, Zao W, Venkataramanan R, Ellis EC, Geller D, Breite AG, Dwulet FE, McCarthy RC, Strom SC Abstract Human hepatocyte transplantation is gaining acceptance for the treatment of liver diseases. However, the reagents used to isolate hepatocytes from liver tissue are not standardized and show lot to lot variability in enzyme activity and endotoxin contamination. For clinical application, highly purified reagents are preferable to crude digest preparations. A purified tissue dissociating enzyme (TDE) preparation (CIzyme™ purified enzymes) was developed based on the enzyme compositions found in a superior lot of collagenase previously used by our group for human hepatocyte isolation. The performance of this enzyme... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5421162 Fri, 11 Nov 2011 05:00:00 +0100 5421162 http://www.medworm.com/index.php?rid=5421161&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22080832%26dopt%3DAbstract This report from CITR presents pre- and post-transplant human leukocyte antigen (HLA) class I sensitization rates in islet alone transplantation. Data came from 303 recipients transplanted with islet alone between January 1999 and December 2008. HLA class I sensitization was determined by the presence of anti-HLA class I antibodies. Panel-reactive antibodies (PRA) from prior to islet infusion and at 6 months, and yearly post-transplant was correlated to measures of islet graft failure. The cumulative number of mismatched HLA alleles increased with each additional islet infusion from a median of 3 for one infusion to 9 for three infusions. Pre-transplant PRA was not predictive of islet graft failure. However, development of PRA ≥20% post-transplant was associated with 3.6 fold (p=0.001) i... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5421161 Fri, 11 Nov 2011 05:00:00 +0100 5421161 http://www.medworm.com/index.php?rid=5421160&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22080915%26dopt%3DAbstract Authors: Soleimanpour SA, Hirshberg B, Bunnell DJ, Sumner AE, Ader M, Remaley AT, Rother KI, Rickels MR, Harlan DM Abstract Although islet transplantation may restore insulin independence to individuals with type 1 diabetes mellitus, most have abnormal glucose tolerance. We asked whether the defective glucose tolerance is due to inadequate β-cell mass or to impaired insulin sensitivity. We performed metabolic studies on four Cynomolgus primates before inducing diabetes with streptozotocin (STZ), then again 2-3 weeks after restoring insulin independence via intrahepatic islet transplantation utilizing a calcineurin inhibitor-free immunosuppressive regimen (induction with rabbit anti-thymocyte globulin and maintenance therapy with rapamycin). Engrafted β-cell mass was assessed by a... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5421160 Fri, 11 Nov 2011 05:00:00 +0100 5421160 http://www.medworm.com/index.php?rid=5345280&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22004871%26dopt%3DAbstract In this study, multiple human MSC transplantations (4.2 x 10⁷ cells/kg each time) were performed intravenously at two-week intervals into streptozocin (STZ)-induced diabetic mice for 6 months. Blood sugar, insulin, renal function, cholesterol and triglyceride levels were monitored. We demonstrated that compared to single intravenous transplantation, which only transiently decreased hyperglycemia, multiple MSC transplantations effectively restored blood glucose homeostasis. Systemic oxidative stress levels were reduced from the 7th week of treatment. From the 11th week, production of human insulin was markedly increased. When MSC transplantation was skipped after blood sugar level returned to normal at the end of 15th week, a sharp rebound of blood sugar occurred, and was then controlled ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5345280 Fri, 14 Oct 2011 04:00:00 +0100 5345280 http://www.medworm.com/index.php?rid=5345279&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22004910%26dopt%3DAbstract Conclusion: Direct myocardial delivery of allogeneic MAPCs two days following AMI through the vessel wall of the infarct related vessel is safe and results in delivery of cells throughout the infarct zone and improved cardiac function despite lack of long-term cell survival. These data further support the hypothesis of cell based myocardial tissue repair by a paracrine mechanism and suggest a clinically translatable strategy for delivering cells at any time after AMI to modulate cardiac remodeling and function. PMID: 22004910 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5345279 Fri, 14 Oct 2011 04:00:00 +0100 5345279 http://www.medworm.com/index.php?rid=5345278&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D22004971%26dopt%3DAbstract Authors: Vieira NM, Valadares M, Zucconi E, Secco M, Bueno CR, Brandalise V, Assoni A, Gomes J, Landini V, Andrade T, Caetano HV, Vainzof M, Zatz M Abstract Duchenne Muscular Dystrophy (DMD), a lethal X-linked disorder, is the most common and severe form of muscular dystrophies, affecting 1 in 3500 male births. Mutations in the DMD gene lead to the absence of muscle dystrophin and a progressive degeneration of skeletal muscle. The possibility to treat DMD through cell therapy has been widely investigated. We have previously shown that human Adipose-derived Stromal Cells (hASCs) injected systemically in SJL mice are able to reach and engraft in the host muscle, express human muscle proteins and ameliorate the functional performance of injected animals without any immunosuppression. ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5345278 Fri, 14 Oct 2011 04:00:00 +0100 5345278 http://www.medworm.com/index.php?rid=5281178&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944319%26dopt%3DAbstract Conclusions: Upregulating post-MI cardiac TIMP-3 expression via cellbased gene therapy contributed additional regulation of MMP, TIMP, and TNF-α levels, thereby boosting the structural and functional effects of VSMCs transplanted at 3 or 14 days after an MI in rats. Early treatment may be superior to late, though both are effective. PMID: 21944319 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281178 Fri, 23 Sep 2011 04:00:00 +0100 5281178 http://www.medworm.com/index.php?rid=5281176&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944493%26dopt%3DAbstract We report here for the first time the derivation of piPSCs from adult fibroblast with only three transcription factors: Sox2 (Sex determining region Y-box 2), Klf4 (Krüppel-like factor 4), and c-Myc (Avian myelocytomatosis viral oncogene homolog). We have been able to demonstrate that exogenous Pou5f1 (POU domain class 5 transcription factor 1; abbreviated as Octamer-4: Oct4) is dispensable to achieve and maintain pluripotency in the generation of piPSCs. To the best of our knowledge, this is also the first report of somatic reprogramming in any species without the overexpression, either directly or indirectly, of Oct4. Moreover, we were able to generate piPSCs without the use of feeder cells, approaching thus xeno-free conditions. Our work paves the way for the derivation of clinical gra... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281176 Fri, 23 Sep 2011 04:00:00 +0100 5281176 http://www.medworm.com/index.php?rid=5281179&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21943517%26dopt%3DAbstract Authors: Airavaara M, Harvey BK, Voutilainen MH, Shen H, Chou J, Lindholm P, Lindahl M, Tuominen RK, Saarma M, Wang Y, Hoffer B Abstract Cerebral dopamine neurotrophic factor (CDNF) is a recently discovered protein, which belongs to the evolutionarily conserved CDNF/MANF family of neurotrophic factors. The degeneration of dopamine neurons following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) -treatment is well characterized and efficacy in this model is considered a standard criterion for development of parkinsonian therapies. MPTP is a neurotoxin, which produces parkinsonian symptoms in humans, and in C57/Bl6 mice. To date, there are no reports about the effects of CDNF on dopamine neuron survival or function in the MPTP rodent model, a critical gap. Therefore, we studied ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281179 Thu, 22 Sep 2011 04:00:00 +0100 5281179 http://www.medworm.com/index.php?rid=5281177&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944459%26dopt%3DAbstract Authors: Serra MP, Doratiotto S, Marongiu F, Laconi E Abstract Cancer often arises in a background of chronic tissue damage. It is also increasingly appreciated that such an injured tissue microenvironment might foster the selective emergence of altered cells, leading to neoplasia. Accordingly, reversal of chronic tissue damage could represent a potential strategy to counteract neoplastic disease. In these studies, we aim to investigate whether transplantation of normal cells in the context of an injured, neoplastic-prone microenvironment might impact on the evolution of carcinogenic process.A rat model of chemically-induced hepatocarcinogenesis was used. Animals were given a single dose of diethylnitrosamine (DENA), followed by two injections of retrorsine (RS), a pyrrolizidine al... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281177 Thu, 22 Sep 2011 04:00:00 +0100 5281177 http://www.medworm.com/index.php?rid=5281175&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944582%26dopt%3DAbstract Conclusion: Atmospheric oxygen delivery by PFOB emulsion might be sufficient to decrease islets hypoxia. However, to improve islets functionality, overoxygenation is needed. Finally, maintenance of islet viability and functionality for several days after isolation could improve the outcome of islets transplantation. PMID: 21944582 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281175 Thu, 22 Sep 2011 04:00:00 +0100 5281175 http://www.medworm.com/index.php?rid=5281174&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944670%26dopt%3DAbstract In this study, we initially generated NPs from human embryonic stem cells (hESCs) and investigated their potential for neuronal differentiation and functional recovery when co-cultured with SCs in vitro and co-transplanted in a rat acute model of contused SCI. Co-cultivation results revealed that the presence of SCs provided a consistent status for hESC-NPs and recharged their neural differentiation toward a predominantly neuronal fate. Following transplantation, a significant functional recovery was observed in all engrafted groups (NPs, SCs, NPs+SCs) relative to the vehicle and control groups. We also observed that animals receiving co-transplants established a better state as assessed with the BBB functional test. Immunohistofluorescence evaluation five weeks after transplantation showe... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281174 Thu, 22 Sep 2011 04:00:00 +0100 5281174 http://www.medworm.com/index.php?rid=5281173&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944777%26dopt%3DAbstract This study was to investigate the effect of trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, on cardiac differentiation of bone marrow mesenchymal stem cells (MSCs) in vitro. Rat MSCs were isolated and divided into 6 groups: 1) control; 2) 5-azacytidine treatment (5-aza, 10 μM); 3) treatment with TSA (100, 300, and 500 nM); 4) treatment with 5-aza followed by incubation with TSA; 5) co-culture with neonatal cardiomyocytes (CMs) ; and 6) treatment with TSA then co-culture with CMs. HDAC activity was significantly inhibited in TSA-treated cells with the maximal inhibition after 24 hrs of exposure to TSA at 300 nM. No changes in HDAC activity were observed in control, 5-aza-treated or co-culture groups. Following 7 days of differentiation, the expression of early cardiac transcr... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281173 Thu, 22 Sep 2011 04:00:00 +0100 5281173 http://www.medworm.com/index.php?rid=5281172&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944862%26dopt%3DAbstract Conclusion: It is possible to restore near normal glucose tolerance with autologous islet transplantation after total pancreatectomy even with sub-optimal number of islets while confirming that islets processed at a remote site are suitable for transplantation. PMID: 21944862 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281172 Thu, 22 Sep 2011 04:00:00 +0100 5281172 http://www.medworm.com/index.php?rid=5281171&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944933%26dopt%3DAbstract This study compares mesenchymal cells isolated from excised burn wound eschar with adipose derived stem cells (ASCs) and dermal fibroblasts in their ability to conform to the requirements for multipotent mesenchymal stem cells. A population of multipotent stem cells, in burn eschar could be an interesting resource for tissue engineering approaches to heal burn wounds. Cells from burn eschar, dermis and adipose tissue were assessed for relevant CD-marker profiles using flowcytometry and for their trilineage differentiation ability in adipogenic, osteogenic and chondrogenic conditions. Although the different cell types did not differ significantly in their CD marker expression, the eschar derived cells and ASCs readily differentiated into adipocytes, osteoblasts and chondrocytes, while derma... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281171 Thu, 22 Sep 2011 04:00:00 +0100 5281171 http://www.medworm.com/index.php?rid=5281170&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21944997%26dopt%3DAbstract EFFECTS OF MYELOABLATION, PERIPHERAL CHIMERISM AND WHOLE. Cell Transplant. 2011 Sep 22; Authors: Lampron A, Lessard M, Rivest S Abstract how bone marrow-derived cells (BMDCs) enter the central nervous system (CNS) is critical for the development of therapies for brainrelated disorders using hematopoietic stem cells. We investigated the brain damages and blood-brain barrier (BBB) modification following either whole-body irradiation or a myeloablative chemotherapy regimen in mice, and the capacity for these treatments to induce the entry of BMDCs into the CNS. Neither treatment had a lasting effect on brain integrity and both were equally efficient at achieving myeloablation. Injection of bone marrow cells from green fluorescent protein (GFP) transgenic mice was able to compl... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5281170 Thu, 22 Sep 2011 04:00:00 +0100 5281170 http://www.medworm.com/index.php?rid=5247193&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929847%26dopt%3DAbstract In this study, we have developed a novel objective method for analysis of immune cells, including Foxp3⁺ T regulatory cell (T reg), on formalin-fixed / paraffin embedded (FFPE) transplant biopsy sections using iCys® Research Imaging Cytometer. The development of multiple immunofluorescent staining was established using FFPE human tonsil sample. The CD4/CD8 ratio and the population of T reg among CD4⁺ cells were analyzed using iCys and compared with the results from conventional flow cytometry analysis (FCM). Our multiple immunofluorescent staining techniques allow obtaining clear staining on FFPE sections. The CD4/CD8 ratio analyzed by iCys was concordant with those obtained by FCM. This method was also applicable for liver, small intestine, kidney, pancreas and heart transplant biops... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247193 Fri, 16 Sep 2011 04:00:00 +0100 5247193 http://www.medworm.com/index.php?rid=5247192&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929848%26dopt%3DAbstract Authors: Gong W, Han Z, Zhao H, Wang Y, Wang J, Zhong J, Wang B, Wang S, Wang Y, Sun L, Han Z Abstract A great deal of interest has arisen recently with respect to human mesenchymal stem cells (MSCs), due to their broad therapeutic potential. However, the safety and efficacy of MSCs expanded ex-vivo for clinical applications remain a concern. In this paper, we establish a standardized process for manufacture of human umbilical cord derived MSCs (UC-MSCs), which encompasses donor screening and testing, recovery, 2-stage expansion, and administration. The biological properties and safety of UC-MSCs were then characterized and tested. The safety data from use in human patients have been also reported. After clinical-scale expansion, a yield of 1.03-3.78× 10⁸ MSCs was achieved in te... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247192 Fri, 16 Sep 2011 04:00:00 +0100 5247192 http://www.medworm.com/index.php?rid=5247191&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929866%26dopt%3DAbstract Authors: Boltze J, Reich DM, Hau S, Reymann KG, Strassburger M, Lobsien D, Wagner DC, Kamprad M, Stahl T Abstract Experimental transplantation of human umbilical cord blood (hUCB) mononuclear cells (MNCs) in rodent stroke models revealed the therapeutic potential of these cells. However, effective cells within the heterogeneous MNC population and their modes of action are still under discussion. MNCs and MNC fractions enriched (CD34⁺) or depleted (CD34⁻) for CD34-expressing stem/progenitor cells were isolated from hUCB. Cells were transplanted intravenously following middle cerebral artery occlusion in spontaneously hypertensive rats and directly or indirectly co-cultivated with hippocampal slices previously subjected to oxygen and glucose deprivation. Application of saline sol... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247191 Fri, 16 Sep 2011 04:00:00 +0100 5247191 http://www.medworm.com/index.php?rid=5247190&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929867%26dopt%3DAbstract CONCLUSIONS: The utilization of collagenase NB1 in human islet isolation was associated with significantly lower β-cell viability, mass and islet potency in vivo in our series when compared to Liberase HI even though there was no significant difference in islet yields between the groups. Evaluation of viable β-cell mass contained in human islet preparations will be useful for selecting enzyme blends. PMID: 21929867 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247190 Fri, 16 Sep 2011 04:00:00 +0100 5247190 http://www.medworm.com/index.php?rid=5247189&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929868%26dopt%3DAbstract In conclusion, Gd-loaded cationic liposomes (125 μM lipid) are an excellent candidate to label cells, without detrimental effects on cell viability, proliferation and differentiation and can be visualized by MRI. PMID: 21929868 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247189 Fri, 16 Sep 2011 04:00:00 +0100 5247189 http://www.medworm.com/index.php?rid=5247188&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929869%26dopt%3DAbstract In this study, we investigate the mechanism for B7-H4-induced prolongation of mouse islet allograft survival. Streptozotocin-induced diabetic C57BL/6 mice were rendered normoglycemic by renal subcapsular implants of B7-H4-transduced BALB/c islets. Grafts and spleens were removed after days 2, 10, and 60 (n=8 each) for characterization of kinetics of Foxp3 and interleukin 10 (IL-10) expression. Mixed lymphocyte reaction (MLR) was done at day 60. Ten mice were subjected to nephrectomy at 60 days and then five were implanted with secondary BALB/c islets and five were given third-party CBA/J islets. An increase in Foxp3 and IL-10 mRNA expression was detected in recipients' spleens at day 60 and this was associated with increased quantities of Foxp3⁺ cells. Splenocytes at day 60 showed hypore... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247188 Fri, 16 Sep 2011 04:00:00 +0100 5247188 http://www.medworm.com/index.php?rid=5247187&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929870%26dopt%3DAbstract Authors: Park D, Joo SS, Kim TK, Lee SH, Kang H, Lee HJ, Lim I, Matsuo A, Tooyama I, Kim YB, Kim SU Abstract Alzheimer disease (AD) is a progressive neurodegenerative disease, which is characterized by loss of memory and cognitive function. In AD patients, dysfunction of the cholinergic system is the main cause of cognitive disorders, and decreased activity of choline acetyltransferase (ChAT), an enzyme responsible for acetylcholine (ACh) synthesis, is observed. In the present study, we investigated if brain transplantation of human neural stem cells (NSCs) genetically modified to encode ChAT gene improves cognitive function of kainic acid (KA)-induced learning deficit rats. Intrahippocampal injection of KA to hippocampal CA3 region caused severe neuronal loss, resulting in profoun... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247187 Fri, 16 Sep 2011 04:00:00 +0100 5247187 http://www.medworm.com/index.php?rid=5247186&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929871%26dopt%3DAbstract Authors: Ratzka A, Kalve I, Ozer M, Nobre A, Wesemann M, Jungnickel J, Köster-Patzlaff C, Baron O, Grothe C Abstract Exogenous cell replacement represents a potent treatment option for Parkinson's disease. However, the low survival rate of transplanted dopaminergic neurons (DA) calls for methodological improvements. Here we evaluated a method to combine transient genetic modification of neuronal progenitor cells with an optimized cell culture protocol prior to intrastriatal transplantation into 6-hydroxydopamine (6-OHDA) unilateral lesioned rats. Plasmid-based delivery of brain-derived neurotrophic factor (BDNF) increases the number of DA neurons, identified by tyrosine hydroxylase immunoreactivity (TH-ir), by 25% in vitro, compared to enhanced green fluorescence protein (EGFP) tr... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247186 Fri, 16 Sep 2011 04:00:00 +0100 5247186 http://www.medworm.com/index.php?rid=5247185&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929872%26dopt%3DAbstract Authors: Chen KY, Wu CC, Chang CF, Chen YH, Chiu WT, Lou YH, Chen YH, Shih HM, Chiang YH Abstract Etk/Bmx (epithelial and endothelial tyrosine kinase, also known as BMX), a member of the Tec (tyrosine kinase expressed in hepatocellular carcinoma) family of protein-tyrosine kinases, is an important regulator of signal transduction for the activation of cell growth, differentiation and development. We have previously reported that activation of Etk leads to apoptosis in MDA-MB-468 cells. The purpose of this study was to examine the role of Etk in neuronal injury induced by H2O2 or ischemia. Using Western blot analysis and immunohistochemistry, we found that treatment with H2O2 significantly enhanced phosphorylation of Etk and its downstream signaling molecule Stat1 in primary cortica... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247185 Fri, 16 Sep 2011 04:00:00 +0100 5247185 http://www.medworm.com/index.php?rid=5247184&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21929873%26dopt%3DAbstract Authors: Fukui T, Matsumoto T, Mifune Y, Shoji T, Kuroda T, Kawakami Y, Kawamoto A, Ii M, Kawamata S, Kurosaka M, Asahara T, Kuroda R Abstract We previously reported the therapeutic potential of human peripheral blood (hPB) CD34+ cells for bone fracture healing via vasculogenesis/angiogenesis and osteogenesis. Not only hPB CD34+ cells but also hPB total mononuclear cells (MNCs) transplantation have shown their therapeutic efficiency for enhancing ischemic neovascularization. Compared with transplantation of purified hPB CD34+ cells, hPB MNCs transplantation is more attractive due to its simple method of cell isolation and inexpensive cost performance in the clinical setting. Thus, in this report, we attempted to test a hypothesis that Granulocyte Colony Stimulating Factor-mobilized... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=5247184 Fri, 16 Sep 2011 04:00:00 +0100 5247184 http://www.medworm.com/index.php?rid=4961462&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669042%26dopt%3DAbstract Authors: Liu KJ, Wang CJ, Chang CJ, Hu HI, Hsu PJ, Wu YC, Bai CH, Sytwu HK, Yen BL Interactions between maternal natural killer lymphocytes (NKs) and fetal tissues are important in mediating maternal-fetal tolerance. We therefore investigated the interactions of NKs to placenta-derived multipotent cells (PDMCs) isolated from the term human placenta. PDMCs have similar cell surface marker expression as BMMSCs and additionally express human embryonic stem cell markers SSEA-4 and CD-9. Differentiation into the tri-mesodermal lineages of osteoblastic, adipocytic, and chondrogenic phenotypes can be readily achieved under the appropriate conditions. We found that PDMCs are more resistant to NK-mediated lysis than the Major Histocompatibility Complex (MHC) class-I null target cell K562, and c... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961462 Wed, 08 Jun 2011 23:00:00 +0100 4961462 http://www.medworm.com/index.php?rid=4961461&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669043%26dopt%3DAbstract Authors: Takita M, Matsumoto S, Qin H, Noguchi H, Shimoda M, Chujo D, Itoh T, Sugimoto K, Onaca N, Naziruddin B, Levy MF One endpoint of clinical islet cell transplantation for type 1 diabetic patients is the elimination or reduction of hypoglycemia. We previously developed a simple tool to evaluate islet graft function; the secretory unit of islet transplant objects (SUITO) index. The aim of this study is to clarify the association between the SUITO index and hypoglycemic episodes. Data from 310 clinical evaluations of eleven islet recipients were included in this study. Fasting plasma C-peptide and glucose levels were measured at every evaluation. The SUITO index was calculated according to the following formula: 1500 x C-peptide level [ng/mL]/(blood glucose level [mg/dL] -63). The n... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961461 Wed, 08 Jun 2011 23:00:00 +0100 4961461 http://www.medworm.com/index.php?rid=4961460&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669044%26dopt%3DAbstract We report here a novel method to derive high-purity definitive endoderm (DE) from hpSCs, based on reproducing features of the normal human embryonic microenvironment. The method mimics the developmental process of transition through a primitive streak, using a differentiation device that incorporates a three-dimensional extracellular matrix (ECM) combined with a porous membrane. Treatment of undifferentiated hpSCs above the membrane results an epithelial-to-mesenchymal transition (EMT), thus responsive cells acquire the ability to migrate through the membrane into the ECM, where they differentiate into definitive endoderm (DE). Importantly, the resultant DE is highly purified, and is not contaminated by undifferentiated cells, as assessed by OCT4 expression using immunocytochemistry and fl... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961460 Wed, 08 Jun 2011 23:00:00 +0100 4961460 http://www.medworm.com/index.php?rid=4961459&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669045%26dopt%3DAbstract In conclusion, f-LSCs represent a possible relevant source of autologous, transplantable, insulin-producing cells which could be tested for the reversal of diabetes. PMID: 21669045 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961459 Wed, 08 Jun 2011 23:00:00 +0100 4961459 http://www.medworm.com/index.php?rid=4961458&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669046%26dopt%3DAbstract Conclusion: the survival of donor cells may have a close relation to not only early integration into hepatic plates but also the differentiated state of the cells at the time of transplantation. PMID: 21669046 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961458 Wed, 08 Jun 2011 23:00:00 +0100 4961458 http://www.medworm.com/index.php?rid=4961457&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669047%26dopt%3DAbstract Authors: Hefferan MP, Johe K, Feldman EL, Lunn JS, Marsala M Previous rodent studies employing mono- or combined immunosuppressive regimens have demonstrated a variable degree of spinal xenograft survival in several spinal neurodegenerative models including spinal ischemia, trauma, or amytrophic lateral sclerosis (ALS). Accordingly, the characterization of optimal immunosuppressive protocols for the specific neurodegenerative model is critical to ensure reliable assessment of potential long-term therapeutic effects associated with cell replacement. In the present study, we characterized the survival of human spinal stem cells when grafted into the lumbar spinal cords of a rodent model of ALS, SOD1 (G93A) male and female rats (60-67 days old). Four different immunosuppressive protocols ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961457 Wed, 08 Jun 2011 23:00:00 +0100 4961457 http://www.medworm.com/index.php?rid=4961456&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669048%26dopt%3DAbstract In this study, the cold-stored sheep carotid artery is used as a substrate to determine the conditions which maximize EC adherence following seeding. The effect of serum concentration, duration of seeding incubation, seeding density and period of cold storage on attachment of EC following seeding of 4-week cold-stored sheep carotid arteries (n=5 arteries), 8-week cold-stored sheep carotid arteries (n=5 arteries) and 12-week cold-stored sheep carotid arteries (n=5 arteries) was examined.Three experiments (serum concentration, time of incubation and seeding density) were conducted to determine the conditions that maximised the number of cultured sheep carotid artery EC that attached to cold-stored sheep carotid artery following seeding. A flat sheet model was used. Serum concentration (%, 10... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961456 Wed, 08 Jun 2011 23:00:00 +0100 4961456 http://www.medworm.com/index.php?rid=4961455&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669049%26dopt%3DAbstract Authors: Kucharova K, Hefferan MP, Patel P, Marsala S, Nejime T, Miyanohara A, Marsala M, Drummond JC A major limitation of neural transplantation studies is assessing the degree of host-graft interaction. In the present study, rat hippocampal/cortical embryonic neurons (E18) were infected with a lentivirus encoding enhanced green fluorescent protein (GFP) under control of the neuron-specific synapsin promoter thus permitting robust identification of labeled neurons after in vivo grafting. Two weeks after transient forebrain ischemia or sham surgery, GFP-expressing neurons were transplanted into CA1 hippocampal regions in immunosuppressed adult Wistar rats. The survival, distribution, phenotype, and axonal projections of GFP-immunoreactive (IR) positive transplanted neurons were evalua... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961455 Wed, 08 Jun 2011 23:00:00 +0100 4961455 http://www.medworm.com/index.php?rid=4961473&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669031%26dopt%3DAbstract In this study, we hypothesize that BMMNCs implanted in cardiomyogenically undifferentiated state to myocardial infarction site would differentiate cardiomyogenically in situ when exogenous TGF-β1 is delivered to the cell implantation site. Heparin-conjugated poly(lactic-co-glycolic acid) nanospheres (HCPNs) suspended in fibrin gel were used as a TGF-β1 delivery system. BMMNCs were labeled with a green fluorescent dye (PKH-67) and implanted into the infarction border zone of rat myocardium using fibrin gel containing HCPNs and TGF-β1. BMMNC implantation using fibrin gel and HCPNs without TGF-β1 served as a control. Four weeks after implantation, the expression of cardiomyogenic marker proteins by the implanted BMMNCs was dramatically greater in the TGF-β1 delivery group than in the con... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961473 Mon, 06 Jun 2011 23:00:00 +0100 4961473 http://www.medworm.com/index.php?rid=4961472&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669032%26dopt%3DAbstract In this study, we aimed to characterize storage injury to human hepatocytes and develop a customized solution for cold storage of these cells. Human hepatocytes were isolated from material obtained from partial liver resections, seeded in monolayer cultures and, after a pre-culture period, stored in the cold in classical and new solutions followed by rewarming in cell culture medium.Human hepatocytes displayed cold-induced injury, resulting in > 80% cell death (LDH release) after one week of cold storage in University of Wisconsin solution or cell culture medium and 3 h of rewarming. Cold-induced injury could be significantly reduced by the addition of the iron chelators deferoxamine and LK 614. Experiments with modified solutions based on the new organ preservation solution Custodiol-N... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961472 Mon, 06 Jun 2011 23:00:00 +0100 4961472 http://www.medworm.com/index.php?rid=4961471&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669033%26dopt%3DAbstract Conclusion: In use of the new human NK cell line, NKG would a promising cellular candidate for adoptive immunotherapy of human cancer. PMID: 21669033 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961471 Mon, 06 Jun 2011 23:00:00 +0100 4961471 http://www.medworm.com/index.php?rid=4961470&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669034%26dopt%3DAbstract This study demonstrated that co-culturing stem cells with primary neurons in vitro decreased neuronal cell death after hypoxia with significantly altered protein secretion. The results suggest that stem cells may offer neuronal protection through extracellular signaling. PMID: 21669034 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961470 Mon, 06 Jun 2011 23:00:00 +0100 4961470 http://www.medworm.com/index.php?rid=4961469&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669035%26dopt%3DAbstract Authors: Lundberg J, Södersten E, Sundström E, Blanc KL, Andersson T, Hermanson O, Holmin S Stem cell transplantation procedures using intraparenchymal injections cause tissue injury in addition to associated surgical risks. Intra-venous cell administration give engraftment in parenchymal lesions although the method has low efficacy and specificity. In pathological conditions with inflammation, such as traumatic brain injury, there is a transient up-regulation of ICAM-1 and VCAM-1 which might provide enviromental cues for migration of stem cells from blood to parenchyma. The aim of this study was to i) analyze the effect of intra-arterial administration on cellular engraftment, ii) compare engraftment and side effects between three different stem cell systems and iii) analyze gene ex... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961469 Mon, 06 Jun 2011 23:00:00 +0100 4961469 http://www.medworm.com/index.php?rid=4961468&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669036%26dopt%3DAbstract Authors: de la Garza-Rodea AS, van der Velde-van Dijke L, Boersma H, Gonçalves MA, van Bekkum DW, de Vries AA, Knaän-Shanzer S Mesenchymal stem cells (MSCs) of mammals have been isolated from many tissues and are characterized by their aptitude to differentiate into bone, cartilage and fat. Differentiation into cells of other lineages like skeletal muscle, tendon/ligament, nervous tissue and epithelium has been attained with MSCs derived from some tissues. Whether such abilities are shared by MSCs of all tissues is unknown. We therefore compared for three human donors the myogenic properties of MSCs from adipose tissue (AT), bone marrow (BM) and synovial membrane (SM). Our data show that human MSCs derived from the three tissues differ in phenotype, proliferation capacity and differe... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961468 Mon, 06 Jun 2011 23:00:00 +0100 4961468 http://www.medworm.com/index.php?rid=4961467&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669037%26dopt%3DAbstract This study investigated whether antisense RNA-mediated TF gene knockdown in islets could suppress IBMIR as a strategy to overcome IBMIR. Neonatal porcine islet cell clusters (NICC) were transfected with or without TF specific antisense RNA or a nonspecific RNA by a lipid-based method. Expression of both TF gene and protein in NICC was analyzed after transfection by real-time PCR, Western blot and FACS, respectively. The impact of antisense RNA transfection on NICC viability and in vitro function was examined by FACS and insulin release test, respectively. The effect of TF knockdown in NICC on IBMIR was assessed with an in vitro tubing loop assay using human blood. A significant reduction in TF gene and protein expression was achieved in TF antisense RNA but not control RNA transfected NICC... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961467 Mon, 06 Jun 2011 23:00:00 +0100 4961467 http://www.medworm.com/index.php?rid=4961466&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669038%26dopt%3DAbstract Authors: Bal G, Kamhieh-Milz J, Futschik M, Haupl T, Salama A, Moldenhauer A Endothelial cells can be successfully used to maintain or increase the number of hematopoietic stem cells in vitro. Previously we identified hematopoietic progenitor cell (HPC) expansion or survival benefit induced by IL-1ß, IL-3 and IL-6 stimulated human umbilical vein endothelial cells (HUVECs) supernatants. In order to identify molecular mechanisms that support hematopoiesis, we examined the time dependent expression profiles of IL-1 ß, IL-3 and IL-6 stimulated HUVECs via microarray. Here, we present 24 common up-regulated elements and three common down-regulated elements of a of IL-ß and IL-3 stimulated HUVECs, with these factors exhibiting great potential for the observed HPC expansion. Furthermore, me... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961466 Mon, 06 Jun 2011 23:00:00 +0100 4961466 http://www.medworm.com/index.php?rid=4961465&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669039%26dopt%3DAbstract In conclusion, AT-EC reach clinically significant cell numbers after few population doublings and are easily accessible from autologous AT, which also contains mesenchymal stem cells/pericytes. Thus AT yields two cell populations which may be used together in the treatment of tissue ischemia and in clinical applications of tissue engineering. PMID: 21669039 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961465 Mon, 06 Jun 2011 23:00:00 +0100 4961465 http://www.medworm.com/index.php?rid=4961464&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669040%26dopt%3DAbstract In conclusion, we describe anti-inflammatory and cytoprotective properties of Exenatide in human islets. Exenatide-mediated PI-9 expression, the only known Granzyme B inhibitor, unveils potential immunoregulatory properties. PMID: 21669040 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961464 Mon, 06 Jun 2011 23:00:00 +0100 4961464 http://www.medworm.com/index.php?rid=4961463&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21669041%26dopt%3DAbstract This study examined whether functional neurons could be efficiently generated from iPS cells using a five-step induction procedure combined with docosahexaenoic acid (DHA) treatment. We demonstrated that DHA, a ligand for the RXR/Nurr1 heterodimer, significantly activated expression of the Nurr1 gene and the Nurr1-related pathway in iPS cells. DHA treatment facilitated iPS differentiation into tyrosine hydroxylase (TH)-positive neurons in vitro and in vivo and functionally increased dopamine release in transplanted grafts in PD-like animals. Furthermore, DHA dramatically up-regulated the endogenous expression levels of neuroprotective genes (Bcl-2, Bcl-xl, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor) and protected against 1-methyl-4-phenyl-1,2,3,6-tetrahyd... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4961463 Mon, 06 Jun 2011 23:00:00 +0100 4961463 http://www.medworm.com/index.php?rid=4812753&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21535908%26dopt%3DAbstract Authors: Wetzig A, Mackay-Sim A, Murrell W There is worldwide enthusiasm for the prospect of some kind of cellular transplant therapy for repair of failing organs. The olfactory mucosa of a patient´s nose is easily biopsied to provide a ready source of multipotent cells. In this paper we address practical issues pertinent to using olfactory neural stem cells for tissue repair. These cells are emerging as potentially most significant candidates for human tissue repair strategies. Previously we have shown that stem cells from olfactory mucosa are multipotent. As well, we have published three potential clinical applications recently. Their expression of dopaminergic markers in vitro and in a Parkinson´s rat transplant model has been demonstrated. Their conversion to chondrogenic phenoty... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812753 Thu, 28 Apr 2011 23:00:00 +0100 4812753 http://www.medworm.com/index.php?rid=4812752&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21535909%26dopt%3DAbstract Authors: Gerard C, Forest MA, Beauregard G, Skuk D, Tremblay JP Duchenne muscular Dystrophy (DMD) is the most frequent muscular dystrophy in children and young adults. Currently, there is no cure for the disease. The transplantation of healthy myoblasts is an experimental therapeutic strategy, since it could restore the expression of dystrophin in DMD muscles. Nevertheless, this cellular therapy is limited by immune reaction, low migration of the implanted cells and high early cell death that could be at least partially due to anoikis. To avoid the lack of attachment of the cells to an extracellular matrix after the transplantation, which is the cause of anoikis, we tested the use of a fibrin gel for myoblast transplantation. In vitro, three concentrations of fibrinogen were compared (... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812752 Thu, 28 Apr 2011 23:00:00 +0100 4812752 http://www.medworm.com/index.php?rid=4812751&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21535910%26dopt%3DAbstract This study investigated the expression of the Bcl-2 family of molecules in isolated human islets. RNA and protein lysates were extracted from human islets immediately post-isolation. At the same time, standard quality control assays including viability staining and beta cell content were performed on each islet preparation. Microarrays, RT-PCR and western blotting were performed on islet RNA and protein. The pro-survival molecules Bcl-xl and Mcl-1, but not Bcl-2 were highly expressed. The multi-domain pro-apoptotic effector molecule Bax was expressed at higher levels than Bak. Pro-apoptotic BH3-only molecules were expressed at low levels, with Bid being the most abundant. The pro-apoptotic molecules BNIP3, BNIP3L and Beclin-1 were all highly expressed indicating exposure of islets to oxyge... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812751 Thu, 28 Apr 2011 23:00:00 +0100 4812751 http://www.medworm.com/index.php?rid=4812750&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21535911%26dopt%3DAbstract In this study, we have investigated the potential of human CD47 expression to promote porcine cell survival in vivo. Human CD47-expressing and control porcine B-lymphoma cells were transplanted into T and B cell-deficient non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice that express SIRPα capable of interacting with human CD47. Only the human CD47-expressing porcine lymphoma cells survived and were able to form tumors in NOD/SCID mice; however, both the control and human CD47-expressing porcine cells survived in macrophage-depleted NOD/SCID mice. These results indicate that transgenic expression of human CD47 may provide an effective approach to inhibiting macrophage-mediated xenograft rejection in clinical xenotransplantation. PMID: 21535911 [PubMed - as supplied by... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812750 Thu, 28 Apr 2011 23:00:00 +0100 4812750 http://www.medworm.com/index.php?rid=4812749&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21535912%26dopt%3DAbstract Authors: Fakhfakh R, Lamarre Y, Skuk D, Tremblay JP Duchenne muscular dystrophy is a recessive X-linked genetic disease caused by dystrophin gene mutations. Cell therapy can be a potential approach aiming to introduce a functional dystrophin in the dystrophic patient myofibers. However, this strategy produced so far limited results. Transforming growth factor β (TGF-β) is a negative regulator of skeletal-muscle development and is responsible for limiting myogenic regeneration. The combination of TGF-β signaling inhibition with myoblast transplantation can be an effective therapeutic approach in dystrophin deficient patients. Our aim was to verify whether the success of human myoblast transplantation in immunodeficient dystrophic mice is enhanced with losartan, a molecule that down-r... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812749 Thu, 28 Apr 2011 23:00:00 +0100 4812749 http://www.medworm.com/index.php?rid=4812758&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21457612%26dopt%3DAbstract Authors: Beltrão-Braga PI, Pignatari GC, Maiorka PC, Oliveira NA, Lizier NF, Wenceslau CV, Miglino MA, Muotri AR, Kerkis I Induced pluripotent stem cells (iPSC) can be created by forcing expression of certain genes in fibroblasts or other somatic cell types, reversing them to a pluripotent state similar to that of embryonic stem cells (ESC). Here, we used human immature dental pulp stem cells (hIDPSC) as an alternative source for creating iPSC. HIDPSC can be easily isolated from accessible tissue of young and adult patients. HIDPSC possess a fibroblast-like morphology, retaining characteristics of adult multipotent stem cells. Reprogramming of hIDPSC was fast, producing primary hIDPSC-iPSC colonies even under feeder-free conditions. hIDPSC acquired ESC-like morphology, expressed pluri... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812758 Thu, 31 Mar 2011 23:00:00 +0100 4812758 http://www.medworm.com/index.php?rid=4812757&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21457613%26dopt%3DAbstract ANGIOGRAPHIC DEMONSTRATION OF NEOANGIOGENESIS AFTER INTRA-ARTERIAL INFUSION OF AUTOLOGOUS BONE MARROW MONONUCLEAR CELLS IN DIABETIC PATIENTS WITH CRITICAL LIMB ISCHAEMIA. Cell Transplant. 2011 Apr 1; Authors: Ruiz-Salmeron R, de la Cuesta-Diaz A, Constantino-Bermejo M, Pérez-Camacho I, Marcos-Sánchez F, Hmadcha A, Soria B Critical limb ischaemia in diabetic patients is associated with high rates of morbidity and mortality. Sub-optimal responses to the available medical and surgical treatments are common in these patients, who also demonstrate limited vascular homeostasis. Neovasculogenesis induced by stem cell therapy could be a useful approach for these patients. Neovasculogenesis and clinical improvement were compared at baseline and at 3 and 12 months after autologous bone... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812757 Thu, 31 Mar 2011 23:00:00 +0100 4812757 http://www.medworm.com/index.php?rid=4812756&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21457614%26dopt%3DAbstract Authors: Kondziolka D, Gobbel GT, Fellows-Mayle W, Chang YF, Uram M The technique of central nervous system cell implantation can affect the outcome of preclinical or clinical studies. Our goal was to evaluate the impact of various injection parameters that may be of consequence during the delivery of solute-suspended cells. These parameters included (1) the type and concentration of cells used for implantation, (2) the rate at which cells are injected (flow rate), (3) the acceleration of the delivery device, (4) the period of time between cell loading and injection into the CNS (delay), and (5) the length and gauge of the needle used to deliver the cells. Neural progenitor cells (NPCs) and bone marrow stromal cells (BMSC) were injected an automated device. These parameters were assess... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812756 Thu, 31 Mar 2011 23:00:00 +0100 4812756 http://www.medworm.com/index.php?rid=4812755&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21457615%26dopt%3DAbstract Authors: Marek N, Bieniaszewska M, Krzystyniak A, Juscinska J, Mysliwska J, Witkowski P, Hellmann A, Trzonkowski P Ex vivo expanded CD4+CD25highCD127- T regulatory cells (Tregs) are recognized as a promising candidate for immunosuppressive therapy in humans. However, due to the plasticity of Tregs lineage and artificial environment present during ex vivo expansion, Tregs easily lose suppressive activity. Here, we followed expanding CD4+CD25highCD127- Tregs and their naive (CD45RA+) and memory-like (CD45RA-) subsets in order to establish the best conditions of the expansion. We found that, regardless of the phenotype sorted, expanding Tregs were undergoing changes resembling homeostatic proliferation and transformed into effector memory-like cells which produced not only suppressive int... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812755 Thu, 31 Mar 2011 23:00:00 +0100 4812755 http://www.medworm.com/index.php?rid=4812754&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21457616%26dopt%3DAbstract Authors: Puppi J, Strom SC, Hughes RD, Bansal S, Castell JV, Dagher I, Ellis EC, Nowak G, Ericzon BG, Fox IJ, Gómez-Lechón MJ, Guha C, Gupta S, Mitry RR, Ohashi K, Ott M, Reid LM, Roy-Chowdhury J, Sokal E, Weber A, Dhawan A On September 6th and 7th, 2009 a meeting was held in London to identify and discuss what are perceived to be current roadblocks to effective hepatocyte transplantation as it is currently practiced in the clinics and, where possible, to offer suggestions to overcome the blocks and improve the outcomes for this cellular therapy. Present were representatives of most of the active clinical hepatocyte transplant programs along with other scientists who have contributed substantial basic research to this field. Over the 2 day sessions based on the experience of the part... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4812754 Thu, 31 Mar 2011 23:00:00 +0100 4812754 http://www.medworm.com/index.php?rid=4646959&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396234%26dopt%3DAbstract Authors: Lin SZ PMID: 21396234 [PubMed - in process] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646959 Tue, 29 Mar 2011 20:45:06 +0100 4646959 http://www.medworm.com/index.php?rid=4646958&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396235%26dopt%3DAbstract Authors: Ding DC, Shyu WC, Lin SZ Stem cells have two features: the ability to differentiate along different lineages and the ability of self-renewal. Two major types of stem cells have been described, namely, embryonic stem cells and adult stem cells. Embryonic stem cells (ESC) are obtained from the inner cell mass of the blastocyst and are associated with tumorigenesis, and the use of human ESCs involves ethical and legal considerations. The use of adult mesenchymal stem cells is less problematic with regard to these issues. Mesenchymal stem cells (MSCs) are stromal cells that have the ability to self-renew and also exhibit multilineage differentiation. MSCs can be isolated from a variety of tissues, such as umbilical cord, endometrial polyps, menses blood, bone marrow, adipose tissu... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646958 Tue, 29 Mar 2011 20:45:06 +0100 4646958 http://www.medworm.com/index.php?rid=4646953&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21439132%26dopt%3DAbstract Authors: Marazzi M, Crovato F, Bucco M, Sironi MC, Tosca MC, Antonioli B, Chlapanidas T, Lucconi G, Scalise A, Torre MF Human hair follicle cells, both bulge and dermal papilla cells, were isolated and cultured in a GMP cell factory, in order to obtain an in vitro hair follicle source for encapsulation end transplantation in alopecia regenerative cell therapy. An in vitro model, constituted by organotypic cultures of human skin sample, was set up to simulate the dermal-epidermal interaction between bulge cells and dermal papilla cells, evaluating the possible new follicles formation and the regenerative potentiality of these hair follicle cells. Both the bulge and dermal papilla cells show an excellent cellular proliferation as well as an abundant extracellular matrix production. The i... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646953 Fri, 25 Mar 2011 00:00:00 +0100 4646953 http://www.medworm.com/index.php?rid=4646952&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21439133%26dopt%3DAbstract In this study, we investigated the ability of short-term RPM treatment in promoting long-term acceptance (LTA) of MHC-mismatched skin allografts by generating a CD8+ suppressor T cell population. We found that CD4 knockout (KO) mice (in C57BL/6 background, H-2b) can promptly reject DBA/2 (H-2d) skin allografts with mean survival time (MST) being 13 days (P<0.01). However, a short course RPM treatment in these animals induced LTA with graft MST longer than 100 days. Adoptive transfer of CD8+ T cells from LTA group into recombination-activating gene 1 (Rag-1) -deficient mice provided donor-specific protection of DBA/2 skin grafts against co-transferred conventional CD8+ T cells. Functionally active immunoregulatory CD8+ T cells also resided in donor skin allografts. Eighteen percent of CD... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646952 Fri, 25 Mar 2011 00:00:00 +0100 4646952 http://www.medworm.com/index.php?rid=4646956&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21439129%26dopt%3DAbstract Authors: Tiemann AG Cellular cardiomyoplasty (CMP) is a novel therapeutic approach to myocardial injury (MI). Post-MI remodelling of the left ventricle (LV) comprises dilatation and impairment of systolic function and gives rise to progressive hemodynamic deterioration. We aimed to investigate: a) the impact of CMP on global and regional parameters of LV remodeling (LVR) as well as contractile reserve and b) the suitability and validity of different echocardiographic methods in this scenario. Murine ventricular cardiomyocytes (E13.5-E16.5) were transplanted into cryolesioned hearts of male HIM-OF1 mice. Echocardiography was performed at rest 4 and 14 days postoperatively. For quantification of akinetic myocardial mass and contractile reserve 2 weeks postoperatively additionally low-dos... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646956 Thu, 24 Mar 2011 00:00:00 +0100 4646956 http://www.medworm.com/index.php?rid=4646955&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21439130%26dopt%3DAbstract This study extends that work toward the goal of developing NKA by (i) screening in vivo inflammatory and fibrotic responses to acellular biomaterials delivered to healthy rodent renal parenchyma, (ii) evaluating the functionality of renal cell/biomaterial combinations in vitro, (iii) generating NKA Constructs by combining therapeutically-relevant cell populations with biocompatible biomaterial, and (iv) evaluating in vivo neo-kidney tissue development in response to NKA Constructs delivered to healthy rodent renal parenchyma. Gelatin and hyaluronic acid (HA)-based hydrogels elicited the least inflammatory and fibrotic responses in renal parenchyma relative to polycaprolactone (PCL) and poly(lactic-co-glycolic acid) (PLGA) beads or particles and were associated with neo-vascularization and ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646955 Thu, 24 Mar 2011 00:00:00 +0100 4646955 http://www.medworm.com/index.php?rid=4646954&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21439131%26dopt%3DAbstract This study has characterized the immunological properties of CLECs and tested a potential therapeutic application in the treatment of a type 1 diabetes mouse model. PMID: 21439131 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646954 Thu, 24 Mar 2011 00:00:00 +0100 4646954 http://www.medworm.com/index.php?rid=4646963&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396172%26dopt%3DAbstract Authors: Md MI [no abstract]. PMID: 21396172 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646963 Wed, 09 Mar 2011 00:00:00 +0100 4646963 http://www.medworm.com/index.php?rid=4646962&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396173%26dopt%3DAbstract In this study, we present and compare two models of biocompatible, tissue-engineered vascular grafts (TEVG), using chitosan and acellularized rat aortas as options for scaffolds. Human aortic adventitial smooth muscle cells and fibroblasts were seeded onto a fibrin gel and subsequently wrapped around either of the two scaffolds. After several weeks of maturation in standard culturing conditions, the graft models were analyzed and compared by mechanical testing, cell viability, and histology. Histological and viability data showed that both models were viable and developed similarly, with a scaffold surrounded by two layers of cells, the fibroblasts lying on top of the smooth muscle cells. Both models responded to 200mM potassium chloride (KCl) (tensions of 38 ± 3μN, 78 ± 13μN, and 52 �... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646962 Wed, 09 Mar 2011 00:00:00 +0100 4646962 http://www.medworm.com/index.php?rid=4646961&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396174%26dopt%3DAbstract Authors: Smardencas A, Birchall I Blood vessels obtained from cadavers and amputated limbs stored at 4ºC (i.e. cold-stored) potentially represent an economical and readily sourced alternative to autologous vessels and synthetic prostheses for vascular reconstructive surgery. However, cold-stored vessels would need to have a reduced antigenicity and an antithrombogenic autologous endothelial cell (EC) lining before they could function as patent vascular allografts. The aim of this study was to determine the effect of cold storage for 1-16 weeks on the morphology of the ovine carotid artery wall. Ovine carotid arteries (n=6) were rinsed and flushed with 0.9% saline, cut into segments, wrapped in 0.9% saline-soaked gauze and stored at 4ºC for 1, 2, 4, 8, or 16 weeks. Following storage, ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646961 Wed, 09 Mar 2011 00:00:00 +0100 4646961 http://www.medworm.com/index.php?rid=4646960&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396175%26dopt%3DAbstract CONCLUSION: Systemically infused hUC-MSCcould home to the inflamed colon and effectively ameliorate colitis. In addition to the known suppressive effects on Th1-type immune responses, hUC-MSC-mediated modulation of IL-23/IL-17 regulated inflammatory reactions also plays an important role in the amelioration of colitis. PMID: 21396175 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646960 Wed, 09 Mar 2011 00:00:00 +0100 4646960 http://www.medworm.com/index.php?rid=4646969&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396166%26dopt%3DAbstract Authors: Pietrosi G, Vizzini G, Luca A, Spada M, Conaldi PG, Triolo F, Gerlach J, Gridelli B [no abstract]. PMID: 21396166 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646969 Tue, 08 Mar 2011 00:00:00 +0100 4646969 http://www.medworm.com/index.php?rid=4646968&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396167%26dopt%3DAbstract Authors: Müeller J, Benz K, Ahlers M, Gaissmaier C, Mollenhauer J Cell-based approaches using mesenchymal stromal progenitor cells (MSCs) for the regeneration of intervertebral discs are attracting increased interest, even though the intervertebral disc is a very demanding environment. Implanted cells eventually face acidic pH, hypoxia, and a lack of nutrients. While the regenerative potential of MSCs for skeletal tissues has been well described, it is still questionable whether human MSCs can be prepared for prolonged survival and proper functioning and whether they can differentiate under the adverse conditions encountered in the disc.Here we examined the influence of hypoxia during expansion and differentiation on the chondrogenesis of MSCs. Chondrogenic differentiation was perform... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646968 Tue, 08 Mar 2011 00:00:00 +0100 4646968 http://www.medworm.com/index.php?rid=4646967&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396168%26dopt%3DAbstract In conclusion, DPP-4i and PPI combination therapy raises endogenous levelsof GLP-1 and gastrin and greatly expands the functional β-cell mass in adult human pancreatic cells implanted in immunodeficient mice, largely from pancreatic duct cells. This suggests that a DPP-4i and PPI combination treatment may provide a pharmacologic therapy to correct the β-cell deficit in type 1 diabetes. PMID: 21396168 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646967 Tue, 08 Mar 2011 00:00:00 +0100 4646967 http://www.medworm.com/index.php?rid=4646966&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396169%26dopt%3DAbstract Authors: Thanos C, Bintz B, Goddard M, Boekelheide K, Hall S, Emerich D One of the primary obstacles in the restoration or repair of damaged tissues is the temporospatial orchestration of biological and physiological events. Cellular transplantation is an important component of tissue repair as grafted cells can serve both as replacement cells or as a source of secreted factors. But few, if any, primary cells can perform more than a single tissue repair function. Epithelial cells, derived from the choroid plexus (CP) are an exception to this rule, as transplanted CP is protective and regenerative in animal models as diverse as CNS degeneration and dermal wound repair. They secrete a myriad of proteins with therapeutic potential as well as matrix and adhesion factors, and contain respon... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646966 Tue, 08 Mar 2011 00:00:00 +0100 4646966 http://www.medworm.com/index.php?rid=4646965&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396170%26dopt%3DAbstract In this study, we compared the telomere length, relative telomerase activity (RTA) and relative reverse transcriptase activity (RRA) as well as the surface antigen profile and mesenchymal differentiation ability in human dental papilla stem cells (DPaSCs) , dental pulp stem cells (DPuSCs) and dental follicle stem cells (DFSCs) with mesenchymal stem cells (MSCs) derived from bone marrow. Dental stem cells (DSCs) were strongly positive for cell surface markers, such as CD44 and CD90. However, slightly lower expression of CD105 was observed in DPaSCs and DPuSCs compared to DFSCs and MSCs. Following specific induction, DPaSCs, DFSCs and MSCs were successfully differentiated into adipocytes and osteocytes. However, DPuSCS, in particular, were able to differentiate into adipocytes but failed to ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646965 Tue, 08 Mar 2011 00:00:00 +0100 4646965 http://www.medworm.com/index.php?rid=4646964&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396171%26dopt%3DAbstract Conclusions: Pancreatic ductal preservation, iodixanol based purification combined with thymoglobulin induction and blockage of IL-1 beta and TNF-alpha as well as sirolimus free immunosuppression dramatically improved the efficacy of clinical islet transplantations. This protocol enabled us to perform successful single-donor islet transplantations. Further large scale studies are necessary to confirm these results and clarify the mechanism of each component. PMID: 21396171 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646964 Tue, 08 Mar 2011 00:00:00 +0100 4646964 http://www.medworm.com/index.php?rid=4646957&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21429290%26dopt%3DAbstract Conclusions: Dogs implanted with autologous SAN cells manifest biological pacing properties dissimilar from those of the anatomic SAN. This highlights the importance of cell and substrate interaction in generating biological pacemaker function. PMID: 21429290 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646957 Tue, 08 Mar 2011 00:00:00 +0100 4646957 http://www.medworm.com/index.php?rid=4647000&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396154%26dopt%3DAbstract Authors: Sgroi A, Mai G, Morel P, Baertschiger RM, Gonelle-Gispert C, Serre-Beinier V, Buhler LH The aim of this study was to evaluate the effects of intraperitoneal transplantation of encapsulated human hepatocytes on liver metabolism and regeneration of mice with acute liver failure. Primary human hepatocytes were immortalized using lentiviral vectors coding for anti-apoptotic genes and microencapsulated using alginate-polylysine polymers. In vitro, immortalized human hepatocytes showed low, but stable synthetic and catabolitic functions over time, when compared to primary hepatocytes. In vivo, mice with acute liver failure and transplanted with encapsulated immortalized human hepatocytes had a significantly improved survival and biochemical profile, compared to mice transplanted wit... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4647000 Mon, 07 Mar 2011 00:00:00 +0100 4647000 http://www.medworm.com/index.php?rid=4646987&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396155%26dopt%3DAbstract We report here a simple and efficient method of generating hESC-derived mesenchymal progenitors (EMPs) and transcriptome profiling using a concise, custom-designed, oligonucleotide gene expression microarray. Characterization of EMPs shows that these cells are similar to BMMSCs in terms of differentiation capacity as well as cell surface marker expression. In addition, EMPs express several ESC markers and HLA-G, a non-classical MHC class I molecule with immunomodulatory properties. Moreover, EMPs possess significantly enhanced proliferative ability over BMMSCs during which karyotypic stability was maintained. Although derived from hESCs, EMPs do not form any tumors in immunocompromised mice. To efficiently profile gene expression in multiple samples, we designed an oligoarray to probe just... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646987 Mon, 07 Mar 2011 00:00:00 +0100 4646987 http://www.medworm.com/index.php?rid=4646986&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396156%26dopt%3DAbstract Authors: Hwang DH, Kim HM, Kang YM, Joo IS, Cho CS, Yoon BW, Kim SU, Kim BG Neural stem cells (NSCs) possess therapeutic potentials to reverse complex pathological processes following spinal cord injury (SCI), but many obstacles remain that could not be fully overcome by NSC transplantation alone. Combining complementary strategies might be required to advance NSC-based treatments to the clinical stage. The present study was undertaken to examine whether combination of NSCs, polymer scaffolds, neurotrophin-3 (NT3), and chondroitinase, which cleaves chondroitin sulfate proteoglycans at the interface between spinal cord and implanted scaffold, could provide additive therapeutic benefits. In a rat hemisection model, poly ( ε -caprolactone) (PCL) was used as a bridging scaffold and as a v... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646986 Mon, 07 Mar 2011 00:00:00 +0100 4646986 http://www.medworm.com/index.php?rid=4646978&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396157%26dopt%3DAbstract In this study, we have isolated MSC from human adipose tissue in culture medium supplemented with either fetal calf serum (FCS) or human platelet lysate (HPL). In addition, culture expansion was simultaneously performed at plating densities of 10 or 10,000 cells/cm². The use of FCS resulted in larger cells, whereas HPL significantly enhanced proliferation. Notably, HPL also facilitated expansion for more population doublings than FCS (43±3 versus 22±4 PD; P<0.001), while plating density did not have a significant effect on long-term growth curves. To gain further insight into population dynamics, we conceived a cellular automaton model to simulate expansion of MSC. It is based on the assumptions that the number of cell divisions is limited and that due to contact inhibition prolifera... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646978 Mon, 07 Mar 2011 00:00:00 +0100 4646978 http://www.medworm.com/index.php?rid=4646977&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396158%26dopt%3DAbstract This study was designed to retrospectively compare the impact of crude Sigma V collagenase (Sigma V, n=52) with high-purified Serva NB1 collagenase (Serva NB1, n=42) on human islet isolation outcomes. A three-step filtration was applied to the crude Sigma V to remove endotoxin contamination and impurities; in addition, this process was used as a lot prescreening tool. Isolation outcomes were determined by digestion efficacy, islet yields, purity, viability, glucose-stimulated insulin release, and endotoxin content. The digestion efficacy between Sigma V and Serva NB1 was statistically significant (Sigma V: 64.71% vs. Serva NB1: 69.71%, p=0.0014). However, the islet yields were similar (Sigma V: 23422.58 vs. Serva NB1: 271097 IEq, p=0.23) between groups. There was no significant purity diff... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646977 Mon, 07 Mar 2011 00:00:00 +0100 4646977 http://www.medworm.com/index.php?rid=4646976&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396159%26dopt%3DAbstract A HIGH FAT/HIGH CHOLESTEROL DIET INHIBITS GROWTH OF FETAL HIPPOCAMPAL TRANSPLANTS VIA INCREASED INFLAMMATION. Cell Transplant. 2011 Mar 7; Authors: Freeman LR, Small BJ, Bickford PC, Umphlet C, Granholm AC A diet containing high levels of saturated fat and cholesterol is detrimental to many aspects of health and is known to lead to obesity, metabolic syndrome, heart disease, diabetes, and cancer. However, the effects of a diet rich in saturated fat and cholesterol on the brain are not currently well understood. In order to determine direct effects of a high saturated fat and cholesterol diet upon fetal hippocampal tissue, we transplanted hippocampal grafts from embryonic day 18 rats to the anterior eye chamber of 16-month old host animals that were fed either a normal rat chow ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646976 Mon, 07 Mar 2011 00:00:00 +0100 4646976 http://www.medworm.com/index.php?rid=4646975&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396160%26dopt%3DAbstract Authors: Kelly CM, Precious SV, Torres EM, Harrison AW, Williams D, Scherf C, Weyrauch UM, Lane EL, Allen ND, Penketh R, Amso NN, Kemp PJ, Dunnett SB, Rosser AE Proof-of-principle' that cell replacement therapy works for neurodegeneration has been reported, but only using donor cells collected from fetal brain tissue obtained from surgical terminations of pregnancy. Surgical terminations of pregnancy represent an increasingly limited supply of donor cells due to the tendency towards performing medical termination in much of Europe. This imposes a severe constraint on further experimental and clinical cell transplantation research. Therefore, we explore here the feasibility of using medical termination tissue as a donor source. Products of conception were retrieved from surgical termina... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646975 Mon, 07 Mar 2011 00:00:00 +0100 4646975 http://www.medworm.com/index.php?rid=4646974&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396161%26dopt%3DAbstract Authors: Chang YS, Choi SJ, Sung DK, Kim SY, Oh W, Yang YS, Park WS Intratracheal transplantation of human umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) attenuates the hyperoxia-induced neonatal lung injury. The aim of this pre-clinical translation study was to optimize the dose of human UCB-derived MSCs in attenuating hyperoxia-induced lung injury in newborn rats. Newborn Sprague-Dawley rats were randomly exposed to hyperoxia (95% oxygen) or normoxia after birth for 14 days. Three different doses of human UCB-derived MSCs, 5×10³ (HT¹), 5×10⁴ (HT²), and 5×10⁵ (HT³), were delivered intratracheally at postnatal day (P) 5. At P14, lungs were harvested for analyses including morphometry for alveolarization, terminal deoxynucleotidyl transferase-mediated deoxyu... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646974 Mon, 07 Mar 2011 00:00:00 +0100 4646974 http://www.medworm.com/index.php?rid=4646973&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396162%26dopt%3DAbstract Authors: Jungebluth P, Luedde M, Ferrer E, Luedde T, Vucur M, Peinado VI, Go T, Schreiber C, Richthofen MV, Bader A, Haag J, Darsow KH, Bartel SJ, Lange HA, Furlani D, Steinhoff G, Macchiarini P Since human lungs are unlikely to repair or regenerate beyond the cellular level, cell therapy has not previously been considered for chronic irreversible obstructive lung diseases. To explore whether cell therapy can restore lung function, we administered allogenic intratracheal mesenchymal stem cell (MSC) in the trachea of rats with chronic thromboembolic pulmonary hypertension (CTEPH), a disease characterized by single or recurrent pulmonary thromboembolic obliteration and progressive pulmonary vascular remodeling. MSCs were retrieved only in high pressure-exposed lungs recruited via a homin... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646973 Mon, 07 Mar 2011 00:00:00 +0100 4646973 http://www.medworm.com/index.php?rid=4646972&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396163%26dopt%3DAbstract Authors: Zeng X, Zeng YS, Ma YH, Lu LY, Du BL, Zhang W, Li Y, Chan WY Three dimensional (3D) gelatin sponge (GS) scaffolds were constructed by ensheathing GS with a thin film of poly-(lactide-co-glycolide) (PLGA). Rat bone marrow-derived mesenchymal stem cells (MSCs) were isolated, cultured and then seeded to the scaffolds. Distribution of cells and cell growth, survival and proliferation within the scaffolds were then determined. Immunofluorescence and Western blot analysis were employed to detect the deposition of fibronectin to the scaffolds on day 3 and day 7 of the culture. Scaffolds with or without MSCs were then transplanted into the transected rat spinal cord. One or eight weeks following transplantation, cavity areas, activated macrophagesαmicroglia, expression of TNF-α and ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646972 Mon, 07 Mar 2011 00:00:00 +0100 4646972 http://www.medworm.com/index.php?rid=4646971&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396164%26dopt%3DAbstract Conclusions. Lysis of MSC is important to take into account when MSC are considered for clinical application. Our results suggest that the HLA background of MSC and timing of MSC administration are important for the efficacy of MSC therapy. PMID: 21396164 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646971 Mon, 07 Mar 2011 00:00:00 +0100 4646971 http://www.medworm.com/index.php?rid=4646970&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21396165%26dopt%3DAbstract In conclusion, the coating of pig islets with ECFCs completely prevented all components of xenogeneic IBMIR. ECFCs may be a better source of protection against xenogeneic IBMIR than are mature ECs. PMID: 21396165 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4646970 Mon, 07 Mar 2011 00:00:00 +0100 4646970 http://www.medworm.com/index.php?rid=4582486&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21375801%26dopt%3DAbstract Authors: Lim JY, Park SI, Kim SM, Jun JA, Oh JH, Ryu CH, Jeong CH, Park SH, Park SA, Oh W, Chang JW, Jeun SS The ability of mesenchymal stem cells (MSCs) to differentiate into neural cells makes them potential replacement therapeutic candidates in neurological diseases. Presently, over-expression of brain-derived neurotrophic factor (BDNF), which is crucial in the regulation of neural progenitor cell differentiation and maturation during development, was sufficient to convert the mesodermal cell fate of human umbilical cord blood-derived MSCs (hUCB-MSCs) into a neuronal fate in culture, in the absence of specialized induction chemicals. BDNF over-expressing hUCB-MSCs (MSCs-BDNF) yielded an increased number of neuron-like cells and, surprisingly, increased the expression of neuronal-phe... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4582486 Fri, 04 Mar 2011 00:00:00 +0100 4582486 http://www.medworm.com/index.php?rid=4582485&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21375802%26dopt%3DAbstract This study provides a method to enhance human PBMNC transplantation-mediated bone formation. PMID: 21375802 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4582485 Fri, 04 Mar 2011 00:00:00 +0100 4582485 http://www.medworm.com/index.php?rid=4582484&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21375803%26dopt%3DAbstract In conclusion, transplantation of cMSCs one week after SCI was more effective in improving clinical signs and neuronal regeneration and reducing fibrosis formation compared to the other transplantation times evaluated. Subsequently, these data may contribute to the optimization of timing for MSC transplantation used as a therapeutic modality. PMID: 21375803 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4582484 Fri, 04 Mar 2011 00:00:00 +0100 4582484 http://www.medworm.com/index.php?rid=4474179&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294943%26dopt%3DAbstract Authors: Ehnert S, Seeliger C, Vester H, Schmitt A, Saidy-Rad S, Lin J, Neumaier M, Gillen S, Kleeff J, Friess H, Burkhart J, Stöckle U, Nussler AK Hepatocyte-transplantation is a therapeutic approach for diverse acute and chronic liver diseases. As availability of primary cells is limited, there is an increasing demand for hepatocyte-like cells, e.g. NeoHepatocytes generated from peripheral blood monocytes. Aim of this study was to evaluate the effects of 6 different human AB sera, fetal calf serum or autologous serum on production of NeoHepatocytes. The yield and quality of NeoHepatocytes varied considerably depending on the different sera. Using autologous sera for the whole production process we constantly generated the highest amount of cells with the highest metabolic activity f... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474179 Thu, 03 Feb 2011 00:00:00 +0100 4474179 http://www.medworm.com/index.php?rid=4474178&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294954%26dopt%3DAbstract Authors: Recio JS, Alvarez-Dolado M, Díaz D, Baltanás FC, Piquer-Gil M, Alonso JR, Weruaga E Many studies have reported the contribution of bone marrow-derived cells (BMDC) to the CNS, raising the possibility of using them as a new source to repair damaged brain tissue or restore neuronal function. This process has mainly been investigated in the cerebellum, in which a degenerative microenvironment has been suggested to be responsible for its modulation. The present study further analyzes the contribution of BMDC to different neural types in other adult brain areas, under both physiological and neurodegenerative conditions, together with the mechanisms of plasticity involved. We grafted genetically marked Green Fluorescent Protein/Cre bone marrow in irradiated recipients: a) The PCD ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474178 Thu, 03 Feb 2011 00:00:00 +0100 4474178 http://www.medworm.com/index.php?rid=4474177&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294955%26dopt%3DAbstract Authors: Ingenito EP, Tsai L, Murthy S, Tyagi S, Mazan M, Hoffman A Autologous lung-derived mesenchymal stem cells (LMSCs) were transplanted endoscopically into sheep with experimental emphysema to assess their capacity to regenerate functional tissue. LMSC lines were derived from transbronchial biopsies, cloned at passage 2, expanded in culture, and labeled. A delivery scaffold containing 1% fibrinogen, 20 μg/mL of fibronectin, and 20 μg/mL of poly-L-lysine was used to promote cell attachment and spreading. Treatment animals received scaffold containing 5-10 × 106 cells/site; control animals received scaffold alone. Phenotypic markers, differentiation capacity, extracellular matrix protein expression, and paracrine function of LMSCs were ocharacterized in vitro. Responses to LMSC t... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474177 Thu, 03 Feb 2011 00:00:00 +0100 4474177 http://www.medworm.com/index.php?rid=4474176&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294956%26dopt%3DAbstract Authors: Ivanovic Z, Duchez P, Chevaleyre J, Vlaski M, Lafarge X, Dazey B, Robert-Richard E, Mazurier F, Boiron JM We developed a clinical-scale cord blood (CB) cell ex-vivo procedure to enable an extensive expansion of committed progenitors - Colony Forming Cells (CFC) without impairing very primitive hematopoietic stem cells (HSC). CD34+ cells, selected from previously cryopreserved and thawed CB units were cultured in two-steps (diluted 1:4 after 6 days) in presence of SCF (Stem Cell Factor), Flt-3L (fms-Related Tyrosine Kinase 3 - Ligand), MGDF (Megakaryocyte Growth and Development factor) (100 ng/ml each), G-CSF (Granulocyte - Colony Stimulating Factor (10 ng/ml) in HP01 serum-free medium. HSC activity has been evaluated in a serial transplantation essay, by detection of human cel... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474176 Thu, 03 Feb 2011 00:00:00 +0100 4474176 http://www.medworm.com/index.php?rid=4474175&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294957%26dopt%3DAbstract Authors: Shin JS, Min BH, Lim JY, Kim BK, Han HJ, Yoon KH, Kim SJ, Park CG Islet transplantation is limited by the difficulties in isolating the pancreatic islets from thecadaveric donor and maintaining them in culture. To increase islet viability and function afterisolation, here we present a novel culture technique involving an histone deacetylase inhibitor(HDACi) to rejuvenate the isolated islets. Pancreatic islets were isolated from Sprague-Dawley (SD) rats and one group (FIs; freshly isolated islets) was used after overnight culture and the other group (RIs; rejuvenated islet) was subjected to rejuvenation culture procedure, which is composed of three discrete steps including degranulation, chromatin remodeling,and regranulation. FIs and RIs were compared with regard to intracellu... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474175 Thu, 03 Feb 2011 00:00:00 +0100 4474175 http://www.medworm.com/index.php?rid=4474174&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294958%26dopt%3DAbstract In conclusion, our data indicate that early post-grafting treatment with PFT-α enhances the survival of dopamine cell transplants and augments behavioral recovery in Parkinsonian animals. PMID: 21294958 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474174 Thu, 03 Feb 2011 00:00:00 +0100 4474174 http://www.medworm.com/index.php?rid=4474173&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294959%26dopt%3DAbstract In conclusion, forced expression of PLAG1 under the control of the Pdx1 or Ngn3 promoter in murine pancreas induces different degrees of endocrine hormone imbalances within the pancreas, which is associated with hypoglycaemia in P1-Pdx1Cre mice but not P1-Ngn3Cre mice. These results suggest that once stem-cell-derived islet transplantations become possible, the appropriate balance between different hormone-producing cells will need to be preserved to prevent deregulated glucose metabolism. PMID: 21294959 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474173 Thu, 03 Feb 2011 00:00:00 +0100 4474173 http://www.medworm.com/index.php?rid=4474172&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294960%26dopt%3DAbstract This study aims at investigating the influence of in vitro chondrogenic differentiation of human adipose tissue-derived stem cells (hATSC) on in vivo cartilage formation when associated with a cellulose-based self-setting hydrogel (Si-HPMC). hATSC were characterized for their proliferation, surface marker expression and multipotency. The in vitro chondrogenic potential of hATSC cultured within Si-HPMC in control or chondrogenic medium was evaluated by measuring COL2A1 and ACAN expression by real time PCR. Alcian blue and type II collagen staining were also performed. To determine whether in vitro chondrogenically-differentiated hATSC may give rise to cartilage in vivo, cells differentiated as a monolayer or in pellets were finally associated with Si-HPMC and implanted subcutaneously into n... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474172 Thu, 03 Feb 2011 00:00:00 +0100 4474172 http://www.medworm.com/index.php?rid=4474171&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294961%26dopt%3DAbstract In conclusion, we show in this study a refined and optimized method for culturing eEPCs. Our data indicate that S-PBMCs are composed of several cell populations including eEPCs and that they secrete high amounts of anti-apoptotic, anti-inflammatory and pro-angiogenic factors capable of preserving cardiac function following MI. PMID: 21294961 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474171 Thu, 03 Feb 2011 00:00:00 +0100 4474171 http://www.medworm.com/index.php?rid=4474170&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294962%26dopt%3DAbstract Authors: Langlois A, Bietiger W, Seyfritz E, Maillard E, Vivot K, Peronet C, Meyer N, Kessler L, Jeandidier N, Pinget M, Sigrist S Delayed and insufficient revascularisation during islet transplantation deprive islets of oxygen and nutrients, resulting in graft failure. VEGF could play a critical role in islet revascularisation. We aimed to develop pharmacological strategies for VEGF overexpression in pancreatic islets using the iron chelator deferoxamine (DFO), thus avoiding obstacles or safety risks associated with gene therapy. Rat pancreatic islets were infected in vivo using an adenovirus (ADE) encoding human VEGF gene (4.10⁸pfu/pancreas) or were incubated in the presence of DFO (10 μmol/L). In vitro viability, functionality and the secretion of VEGF were evaluated in islets 1 ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474170 Thu, 03 Feb 2011 00:00:00 +0100 4474170 http://www.medworm.com/index.php?rid=4474169&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294963%26dopt%3DAbstract This study provides a genome-wide view for the candidate genes of CD4⁺ T cells related to allotransplantation, and this report is a good resource for further microarray studies and for identifying the specific markers that are associated with clinical organ transplantations. PMID: 21294963 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474169 Thu, 03 Feb 2011 00:00:00 +0100 4474169 http://www.medworm.com/index.php?rid=4474168&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21294964%26dopt%3DAbstract Authors: Ock SA, Rho GJ Dimethyl sulfoxide (DMSO), a commonly used cryoprotectant in cryopreservation procedure is detrimental to viability of cells. In this view point, a comparative study was carried out to evaluate the effect of DMSO on porcine mesenchymal stem cells (pMSCs). We compared the viability, colony forming unit-fibroblast (CFU-F) assay, expression of Bak and Bcl2 genes, Bcl2 protein antigen and CD90 in pMSCs cryopreserved with 5, 10 and 20% DMSO. pMSCs isolated from bone marrow were characterized by alkaline phosphatase activity and the expression of transcription factors, such as Oct 3/4, Nanog, and Sox2. The cells were then cryopreserved by cooling at a rate of -1°C/min in a programmable freezer and stored in liquid nitrogen. The results of survival of pMSCs cryopreser... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4474168 Thu, 03 Feb 2011 00:00:00 +0100 4474168 http://www.medworm.com/index.php?rid=4343645&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176394%26dopt%3DAbstract In this study, we have demonstrated the benefits of the conditioned medium derived from human MSC (CM-MSC) in wound healing using an excisional wound model. CM-MSC accelerated wound closure with increased re-epithelialization, cell infiltration, granulation formation, and angiogenesis. Notably, CM-MSC enhanced epithelial and endothelial cell migration, suggesting the contribution of increased cell migration to wound healing enhanced by CM-MSC. Cytokine array, ELISA analysis and quantitative RT-PCR revealed high levels of IL-6 in CM-MSC. Moreover, IL-6 added to the preconditioned medium enhanced both cell migration and wound healing, and antibodies against IL-6 blocked the increase in cell motility and wound closure by CM-MSC. The IL-6 secretory pathway of MSCs was inhibited by SB203580, an... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343645 Wed, 22 Dec 2010 00:00:00 +0100 4343645 http://www.medworm.com/index.php?rid=4343644&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176395%26dopt%3DAbstract Authors: Inagaki M, Furukawa H, Satake Y, Okada Y, Nishikawa SC, Ogawa K Although hepatocyte transplantation (HCTx) is expected to become a useful therapy for human liver diseases, allogenic hepatocytes still tend to be rejected within a short period due to host immunosurveillance. In the present study, we investigated the effect of prior bone marrow transplantation (BMTx) for the engraftment of allogenic hepatocytes using the analbuminemic rat transplantation model. The hepatocytes of Lewis (LEW) rats were not accepted in the liver of Retrorsine (RS)/partial hepatectomy (PH)-treated analbuminemic F344 (F344-alb) rats which express the disparate major histocompatibility complex (MHC) against that of LEW rats. Prior BMTx with the LEW bone marrow cells (BMCs) after sublethal irradiation ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343644 Wed, 22 Dec 2010 00:00:00 +0100 4343644 http://www.medworm.com/index.php?rid=4343643&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176396%26dopt%3DAbstract Authors: Ho JH, Chen YF, Ma WH, Tseng TC, Chen MH, Lee OK Mesenchymal stem cells (MSCs) are of great therapeutic potentials due to their multi-lineage differentiation capabilities. Before transplantation, in vitro culture-expansion of MSCs is necessary to get desired cell number. We observed that cell contact accelerated replicative senescence during such process. To confirm the finding as well as to investigate the underlying mechanisms, we cultured both human bone marrow- and umbilical cord blood-derived MSCs under non-contact culture (subculture performed at 60-70% of confluence), or contact culture (cell passage performed at 100% of confluence). It was found that MSCs reached cellular senescence earlier in contact culture, and the doubling time was significantly prolonged. Marked i... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343643 Wed, 22 Dec 2010 00:00:00 +0100 4343643 http://www.medworm.com/index.php?rid=4343642&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176397%26dopt%3DAbstract Authors: Tatsumi R, Suzuki Y, Sumi T, Sone M, Suemori H, Nakatsuji N Endothelial cells derived from human embryonic stem cells (hESC-ECs) hold much promise as a valuable tool for basic vascular research and for medical application such as cell transplantation or regenerative medicine. Here we have developed an efficient approach for the production of hESC-ECs. Using a differentiation method consisting of a stepwise combination of treatment with glycogen synthase kinase-3β (GSK-3β) inhibitor and culturing in vascular endothelial growth factor (VEGF)-supplemented medium, hESC-ECs are induced in 5 days with about 20% efficiency. These cells express vascular endothelial cadherin (VE-cadherin), VEGF receptor-2 (VEGFR-2), CD34, and platelet endothelial cell adhesion molecule-1 (PECAM-1). T... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343642 Wed, 22 Dec 2010 00:00:00 +0100 4343642 http://www.medworm.com/index.php?rid=4343641&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176398%26dopt%3DAbstract This study investigated whether retrograde infusion via the hepatic vein provides a sufficient number of donor cells for the liver. Donor hepatocytes were isolated from dipeptidyl peptidase IV (DPPIV⁺) rats and transplanted into DPPIV⁻ rat livers either by antegrade portal vein infusion or retrograde hepatic vein infusion. Hepatocyte engraftment ratios and localization were evaluated by histological DPPIV enzymatic staining at 1 week and 8 weeks after the transplantation. No significant differences in engraftment efficiency were observed at either 1 week or 8 weeks after transplantation by either route. However, the localization of the transplanted hepatocytes differed with the administration route. Portal vein infusion resulted in predominantly peri-portal engraftment, whereas hepatic... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343641 Wed, 22 Dec 2010 00:00:00 +0100 4343641 http://www.medworm.com/index.php?rid=4343640&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176399%26dopt%3DAbstract STRATEGIES FOR THE DEVELOPMENT OF CELL LINES FOR EX VIVO GENE THERAPY IN THE CENTRAL NERVOUS SYSTEM. Cell Transplant. 2010 Dec 22; Authors: Mejía-Toiber J, Castillo CG, Giordano M Disorders of the central nervous system (CNS) either as a result of trauma, ischemic or neurodegenerative process, still pose a challenge for modern medicine. Due to the complexity of the CNS, and in spite of the advances in the knowledge of its anatomy, pharmacology, and molecular and cellular biology, treatments for these diseases are still limited. The development of cell lines as a source for transplantation into the damaged CNS (cell therapy), and more recently their genetic modification to favor the expression and delivery of molecules with therapeutic potential (ex vivo gene therapy) are some ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343640 Wed, 22 Dec 2010 00:00:00 +0100 4343640 http://www.medworm.com/index.php?rid=4343639&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176400%26dopt%3DAbstract Authors: Morosetti R, Gidaro T, Broccolini A, Gliubizzi C, Sancricca C, Tonali PA, Ricci E, Mirabella M Facioscapulohumeral Muscular Dystrophy is the third most frequent inherited myopathy. We previously demonstrated that mesoangioblasts can be efficiently isolated from FSHD muscles, although their differentiation ability into skeletal muscle was variably impaired. This correlates with overall disease severity and degree of histopathologic abnormalities, since mesoangioblasts from morphologically normal muscles did not show any myogenic differentiation block. The aim of our present study was to verify whether mesoangioblasts from differentially affected FSHD muscles reproduce in vivo the same differentiation ability shown in vitro by studying their capability to form new muscle fibers ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343639 Wed, 22 Dec 2010 00:00:00 +0100 4343639 http://www.medworm.com/index.php?rid=4343638&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176401%26dopt%3DAbstract In conclusion, the sequential combination of a JNK inhibitor and simvastatin protected porcine islets from peri-transplant apoptosis. Inhibition of JNK reduced the inflammatory response and could be considered an alternative target for suppression of porcine islet inflammation. PMID: 21176401 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343638 Wed, 22 Dec 2010 00:00:00 +0100 4343638 http://www.medworm.com/index.php?rid=4343637&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176402%26dopt%3DAbstract Authors: Jiang W, Büchele F, Papazoglou A, Döbrössy M, Nikkhah G Embryonic striatal graft mediated functional recovery in the rodent lesion model of Huntington's disease (HD) has been shown to correlate with the proportion of dopamine- and adenosine 3',5'-monophosphat-regulated phosphoprotein with a molecular weight of 32 kDa (DARPP-32) positive neurones in the graft. The current study investigated the impact of graft distribution on the yield of DARPP-32 positive cells in the grafts following either single-tract or multi-tract cell delivery protocols using the microtransplantation approach. Cells derived from the whole ganglionic eminence of E15 rat embryos, ubiquitously expressing Green Fluorescent Protein (GFP), were implanted into unilaterally QA-lesioned rat striatum either as ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343637 Wed, 22 Dec 2010 00:00:00 +0100 4343637 http://www.medworm.com/index.php?rid=4343636&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176403%26dopt%3DAbstract In this study, we hypothesized that human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have the multifunctional abilities to ameliorate the NPC symptoms in the brain. To test this hypothesis, hUCB-MSCs were transplanted into the hippocampus of NPC mice in the early asymptomatic stage. This transplantation resulted in the recovery of motor function in the Rota Rod test and impaired cholesterol homeostasis leading to increased levels of cholesterol efflux related genes such as LXRα, ABCA1 and ABCG5 while decreased levels of 3-hydroxy-3-methylglutaryl coenzyme A reductase were observed in NPC mice. In the cerebrum, hUCB-MSCs enhanced neuronal cell survival and proliferation, where they directly differentiated into electrically-active MAP2 positive neurons as demonstrated b... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343636 Wed, 22 Dec 2010 00:00:00 +0100 4343636 http://www.medworm.com/index.php?rid=4343635&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176404%26dopt%3DAbstract This study was undertaken to compare the phenotype of human articular chondrocytes (ACs) and bone marrow-derived mesenchymal stem cells (MSCs) after cell expansion by studying the spectrum of proteins secreted by cells into the culture medium. ACs and MSCs were expanded in monolayer cultures for some weeks, as done in standard cell transplantation procedures. Initially, the expression of cartilage signature genes was compared by real-time PCR. Metabolic labelling of proteins (SILAC) in combination with mass spectrometry (LC/MS-MS) was applied to investigate differences in released proteins. In addition multiplex assays were carried out to quantify the amounts of several matrix metalloproteases (MMPs) and their natural inhibitors (TIMPs). Expanded chondrocytes showed a slightly higher expre... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343635 Wed, 22 Dec 2010 00:00:00 +0100 4343635 http://www.medworm.com/index.php?rid=4343634&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176405%26dopt%3DAbstract Authors: Sattler C, Steinsdoerfer M, Offers M, Fischer E, Schierl R, Heseler K, Aubener WD, Seissler J Multipotent mesenchymal stromal cells (MSCs) are bone marrow derived cells of non-hematopoietic origin with immunoregulatory properties. Although some functions of MSCs have been identified, there are still features which are not explained thus far. The aim of the present study was to identify novel factors involved in MCS mediated inhibition of T cell proliferation. We here demonstrate that the surface molecule CD39 is coexpressed in concert with CD73 on murine MSCs catalyzing the generation of adenosine which can directly act on activated T cells via the adenosine A2A receptor. Blocking of the adenosine pathway either by the A2A receptor antagonist SCH58261 or the specific CD39-inhi... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343634 Wed, 22 Dec 2010 00:00:00 +0100 4343634 http://www.medworm.com/index.php?rid=4343633&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176406%26dopt%3DAbstract In this study we investigated the role of Cytomegalovirus in islet allograft failure. STZ-diabetic rats received an allogenic islet graft in combination with either an acute CMV-infection or control infection. A third group received ganciclovir treatment in addition to the CMV-infection. Graft function was assessed by measuring basal blood glucose levels. After sacrifice, the islet grafts were retrieved for analysis of infection and leukocyte infiltration. CMV-infected recipients demonstrated accelerated islet graft failure as compared to non-infected controls. CMV-infection of the graft was only observed prior to complete graft failure. Quantification of the leukocyte infiltration demonstrated increased CD8⁺ T cell and NK cell infiltration in the CMV-infected grafts, as compared to the ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343633 Wed, 22 Dec 2010 00:00:00 +0100 4343633 http://www.medworm.com/index.php?rid=4343632&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176407%26dopt%3DAbstract Authors: Kuroda R, Matsumoto T, Miwa M, Kawamoto A, Mifune Y, Fukui T, Kawakami Y, Niikura T, Lee SY, Oe K, Shoji T, Kuroda T, Horii M, Yokoyama A, Ono T, Koibuchi Y, Kawamata S, Fukushima M, Kurosaka M, Asahara T Although implantation of crude bone marrow cells has been applied in a small number of patients for fracture healing, transplantation of peripheral blood CD34+ cells, the hematopoietic/ endothelial progenitor cell-enriched population, in patients with fracture has never been reported. Here, we report the first case of tibial nonunion receiving autologous, granulocyte colony stimulating factor mobilized CD34+ cells accompanied with autologous bone grafting. No serious adverse event occurred, and the novel therapy performed 9 months after the primary operation resulted in bone ... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343632 Wed, 22 Dec 2010 00:00:00 +0100 4343632 http://www.medworm.com/index.php?rid=4343631&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176408%26dopt%3DAbstract In this study, the EU FP6-funded CRYSTAL (CRYo-banking of Stem cells for human Therapeutic AppLications) consortium aimed to validate novel Standard Operating Procedures (SOPs) to perform and validate xeno-free and chemically defined cryopreservation of human progenitor cells and to reduce the amount of the potentially toxic cryoprotectant additive (CPA) dimethyl sulfoxide (DMSO). To achieve this goal, three human adult progenitor- and stem cell populations - umbilical cord blood (UCB)-derived erythroid cells (UCB-ECs), UCB-derived endothelial colony forming cells (UCB-ECFCs), and adipose tissue (AT)-derived mesenchymal stromal cells (AT-MSCs) - were cryopreserved in chemically defined medium supplemented with 10% or 5% DMSO. Cell recovery, cell repopulation and functionality were evaluate... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343631 Wed, 22 Dec 2010 00:00:00 +0100 4343631 http://www.medworm.com/index.php?rid=4343630&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21176409%26dopt%3DAbstract In this study we investigated ES cell migration under the influence of various extracellular matrix (ECM) proteins, which have been shown to stimulate cell migration in various cell models with unclear effects on ES cells. Using two mouse ES (mES) cell lines, ESC 26GJ9012-8-2 and ES-D3 GL, to generate embryoid bodies (EB), we examined the migration of differentiating cells from EB that were delivered onto culture surfaces coated with or without collagen I, collagen IV, Matrigel, fibronectin, and laminin. Among these ECM proteins, collagen IV exhibited maximal migration enhancing effect. mES cells expressed α2 and β1 integrin subunits and the migration enhancing effect of collagen IV was prevented by RGD peptides as well as antibodies against α2 and β1 integrins, indicating that the enh... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4343630 Wed, 22 Dec 2010 00:00:00 +0100 4343630 http://www.medworm.com/index.php?rid=4202606&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092402%26dopt%3DAbstract We examined whether MSC survival in the injured spinal cord could be enhanced by closely matching donor and recipient mice for genetic background and marker gene expression and whether exposure of MSC to a neural environment (Schwann cells) prior to transplantation would improve their survival or therapeutic effects. Mice underwent a clip compression spinal cord injury at the fourth thoracic level and cell transplantation 7 days later. Despite genetic matching of donors and recipients, MSC survival in the injured spinal cord was very poor ( ~1%). However we noted improved locomotor recovery accompanied by improved histopathological appearance of the lesion in mice receiving MSC grafts. These mice had more white and gray matter sparing, laminin expression, Schwann cell infiltration and pres... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202606 Fri, 19 Nov 2010 00:00:00 +0100 4202606 http://www.medworm.com/index.php?rid=4202605&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092403%26dopt%3DAbstract In conclusion, the TLM was beneficial for prolonged pancreas preservation before human islet isolation, however, benefit of the TLM for short-term preservation was not clear. PMID: 21092403 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202605 Fri, 19 Nov 2010 00:00:00 +0100 4202605 http://www.medworm.com/index.php?rid=4202604&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092404%26dopt%3DAbstract Authors: Shaw SW, Bollini S, Nader KA, Gastadello A, Mehta V, Filppi E, Cananzi M, Gaspar HB, Qasim W, De Coppi P, David AL Long term engraftment and phenotype correction has been difficult to achieve in humans after in utero stem cell transplantation mainly because of allogeneic rejection. Autologous cells could be obtained during gestation from the amniotic fluid with minimal risk for the fetus and the mother. Using a sheep model, we explored the possibility of using amniotic fluid mesenchymal stem cells (AFMSCs) for autologous in utero stem cell/gene therapy. We collected amniotic fluid (AF) under ultrasound-guided amniocentesis in early gestation pregnant sheep (n=9, 58 days of gestation, term = 145 days). AFMSCs were isolated and expanded in all sampled fetal sheep. Those cells we... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202604 Fri, 19 Nov 2010 00:00:00 +0100 4202604 http://www.medworm.com/index.php?rid=4202603&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092405%26dopt%3DAbstract In this study, we aimed to validate whether intravenous (IV) administration of allogeneic NSC can improve experimental autoimmune encephalomyelitis (EAE), a well-established animal model for human multiple sclerosis (MS). For this, we cultured adherently growing luciferase-expressing NSC (NSC-Luc), which displayed a uniform morphology and expression profile of membrane and intracellular markers, and which displayed an in vitro differentiation potential into neurons and astrocytes. Following labelling with green fluorescent micron-sized iron oxide particles (f-MPIO-labelled NSC-Luc) or lentiviral transduction with the enhanced green fluorescent protein (eGFP) reporter gene (NSC-Luc/eGFP), cell implantation experiments demonstrated the intrinsic survival capacity of adherently cultured NSC i... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202603 Fri, 19 Nov 2010 00:00:00 +0100 4202603 http://www.medworm.com/index.php?rid=4202602&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092406%26dopt%3DAbstract Authors: Crespo-Diaz R, Behfar A, Butler GW, Padley DJ, Sarr MG, Bartunek J, Dietz AB, Terzic A With favorable regenerative and immunotolerant profiles, patient-derived human mesenchymal stem cells (hMSC) are increasingly considered in cell therapy. Derived from bone marrow (BM) and standardized with culture in fetal bovine serum (FBS), translation of hMSC-based approaches is impeded by protracted expansion times, risk of xenogenic response and exposure to zoonoses. Here, human platelet lysate adherent to good manufacturing practices (GMP-hPL) provided a non-zoonotic adjuvant that enhanced the capacity of BM-hMSC to proliferate. The nurturing benefit of GMP-hPL was generalized to hMSC from adipose tissue evaluated as an alternative to bone marrow. Long-term culture in GMP-hPL maintaine... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202602 Fri, 19 Nov 2010 00:00:00 +0100 4202602 http://www.medworm.com/index.php?rid=4202601&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092407%26dopt%3DAbstract Conclusions: The presence of apoptotic cells among BMMCs impairs the efficacy of cardiac cell therapy after MI, an effect possibly mediated by apoptotic microparticles. PMID: 21092407 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202601 Fri, 19 Nov 2010 00:00:00 +0100 4202601 http://www.medworm.com/index.php?rid=4202600&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092408%26dopt%3DAbstract HUMAN AMNION EPITHELIAL CELLS PREVENT BLEOMYCIN-INDUCED LUNG INJURY AND PRESERVE LUNG FUNCTION. Cell Transplant. 2010 Nov 19; Authors: Murphy S, Lim R, Dickinson H, Acharya R, Rosli S, Jenkin G, Wallace E Human amnion epithelial cells (hAECs) have attracted recent attention as a promising source of cells for regenerative therapies, with reports that cells derived from human term amnion possess multipotent differentiation ability, low immunogenicity, and anti-inflammatory properties. Specifically, in animal models of lung disease characterized by significant loss of lung tissue secondary to chronic inflammation and fibrosis, the transplantation of hAECs has been shown to reduce both inflammation and subsequent fibrosis. To further explore the mechanisms by which hAECs reduce pul... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202600 Fri, 19 Nov 2010 00:00:00 +0100 4202600 http://www.medworm.com/index.php?rid=4202599&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092409%26dopt%3DAbstract In this study, we explored the effects of striatal stimulation (SS) in a rat model of chronic-phase ischemic stroke. The stimulation electrode was implanted into the ischemic penumbra at one month after middle cerebral artery occlusion (MCAO) and thereafter continuously delivered SS over a period of one week. Rats were evaluated behaviorally coupled with neuroradiological assessment of the infarct volumes using magnetic resonance imaging (MRI) at pre- and post-SS. The rats with SS showed significant behavioral recovery in the spontaneous activity and limb placement test compared to those without SS. MRI visualized that SS also significantly reduced the infarct volumes compared to that at pre-SS or without SS. Immunohistochemical analyses revealed a robust neurogenic response in rats that r... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202599 Fri, 19 Nov 2010 00:00:00 +0100 4202599 http://www.medworm.com/index.php?rid=4202598&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092410%26dopt%3DAbstract Authors: Even Y, Bennett JL, Sekulovic S, So L, Yi L, McNagny KM, Humphries RK, Rossi FM Gene therapy as well as methods capable of returning cells to a pluripotent state (iPS) have enabled the correction of genetic deficiencies in syngenic adult progenitors, reducing the need for immunosuppression in cell therapy approaches. However in diseases involving mutations that lead to the complete lack of a protein, such as Duchenne muscular dystrophy, the main immunogens leading to rejection of transplanted cells are the therapeutic proteins themselves. In these cases even iPS cells would not circumvent the need for immunosuppression, and alternative strategies must be developed. One such potential strategy seeks to induce immune tolerance using hematopoietic stem cells originated from the s... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202598 Fri, 19 Nov 2010 00:00:00 +0100 4202598 http://www.medworm.com/index.php?rid=4202597&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092411%26dopt%3DAbstract Authors: Petersen TH, Calle EA, Colehour MB, Niklason LE In this paper, we describe the design and validation of a bioreactor for the in vitro culture of whole rodent lung tissue. Many current systems only enable large segments of lung tissue to be studied ex vivo for up to a few hours in the laboratory. This limitation restricts the study of pulmonary biology in controlled laboratory settings, and also impacts the ability to reliably culture engineered lung tissues in the laboratory. Therefore, we designed, built and validated a bioreactor intended to provide sufficient nutrient supply and mechanical stimulation to support cell survival and differentiation in cultured lung tissue. We also studied the effects of perfusion and ventilation on pulmonary cell survival and maintenance of ce... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202597 Fri, 19 Nov 2010 00:00:00 +0100 4202597 http://www.medworm.com/index.php?rid=4202596&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092412%26dopt%3DAbstract In this study, primary human hepatocytes were incubated with increasing concentrations of clinical grade SPIOs for different time intervals. SPIOs uptake was confirmed by light and fluorescence microscopy, and intracellular iron content quantified by a colorimetric ferrozine-based assay. Studies were performed to determine if labelling affected cell viability and function. Intracellular iron concentrations increased in a time and dose-dependent manner after incubation with SPIOs. Labelling had minimal short-term effects on cell attachment and mitochondrial function. However, exposure of hepatocytes to SPIOs resulted in a dose and time-dependent reduction in protein synthesis. Cell labelling for 16 h had no significant effect on hepatocyte specific function, but longer periods of incubation... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202596 Fri, 19 Nov 2010 00:00:00 +0100 4202596 http://www.medworm.com/index.php?rid=4202595&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092413%26dopt%3DAbstract Conclusion: Splenectomy is an effective option for the patients who had pancytopenia after the PBSCT and the patients recovered a good graft function after splenectomy without procedure-related complication and with long-time survival. GVHD can develop in both allo-PBSCT and syngeneic PBSCT. A splenectomy after PBSCT may increase the risk of GVHD, enhance the effect of graft versus leukemia (GVL), promote CDC formation. PMID: 21092413 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202595 Fri, 19 Nov 2010 00:00:00 +0100 4202595 http://www.medworm.com/index.php?rid=4202594&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21092414%26dopt%3DAbstract Authors: Manna GL, Bianchi F, Cappuccilli M, Cenacchi G, Tarantino L, Pasquinelli G, Valente S, Bella ED, Cantoni S, Claudia C, Neri F, Tsivian M, Nardo B, Ventura C, Stefoni S Acute kidney injury (AKI) is a major health care condition with limited current treatment options. Within this context, stem cells may provide a clinical approach for AKI. Moreover, a synthetic compound previously developed, hyaluronan monoesters with butyric acid (HB), able to induce metanephric differentiation, formation of capillary-like structures and secretion of angiogenic cytokines, was tested in vitro. Thereafter, we investigated the effects of human mesenchymal stem cells from fetal membranes (FMhMSCs), both treated and untreated with HB, after induction of ischemic AKI in a rat model. At reperfusion fo... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4202594 Fri, 19 Nov 2010 00:00:00 +0100 4202594 http://www.medworm.com/index.php?rid=4155195&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054927%26dopt%3DAbstract In conclusion, our results indicate that fetal MSCs could be an alternative, more accessible resource for cell therapy and regenerative medicine. PMID: 21054927 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4155195 Fri, 05 Nov 2010 00:00:00 +0100 4155195 http://www.medworm.com/index.php?rid=4155194&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054928%26dopt%3DAbstract In conclusion, we propose the TEL as a state-of-art substitute for whole-liver transplantation and as a proof of concept for the technology that will eventually allow for the transplantation of a reconstituted liver. PMID: 21054928 [PubMed - as supplied by publisher] (Source: Cell Transplantation) Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4155194 Fri, 05 Nov 2010 00:00:00 +0100 4155194 http://www.medworm.com/index.php?rid=4155193&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054929%26dopt%3DAbstract Authors: Wang Y, Nathanson L, McNiece IK During mouse embryogenesis, hematopoietic development takes place in several distinct anatomic locations. The microenvironment of different hematopoietic organs plays an important role in the proliferation and maturation of the hematopoietic cells. We hypothesized that fetal stromal cells would be distinct to adult bone marrow (BM) derived stromal cells since the BM contributes mainly to the homeostasis of hematopoietic stem cells (HSCs), while extensive expansion of HSCs occurs during fetal development. Here we report the establishment of stromal cell lines from fetal hematopoietic organs, namely aorta-gonad-mesonephros (AGM), midgestation placenta (PL) and fetal liver (FL)) together with adult bone marrow (BM). The growth patterns and hematopo... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4155193 Fri, 05 Nov 2010 00:00:00 +0100 4155193 http://www.medworm.com/index.php?rid=4155192&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054930%26dopt%3DAbstract Authors: Takahashi Y, Tsuji O, Kumagai G, Hara CM, Okano HJ, Miyawaki A, Toyama Y, Okano H, Nakamura M To investigate potential cures for spinal cord injury (SCI), several researchers have transplanted neural stem/progenitor cells (NS/PCs) into the injured spinal cord by different procedures, including intralesional (IL), intrathecal (IT), and intravenous (IV) injection. However, there are no reports quantifying or comparing the number of cells successfully transplanted to the lesion site by each procedure in vivo. The purpose of the present study was to determine the optimal method of cell transplantation to the SCI site in terms of grafted cell survival and safety. For this purpose, we developed mouse NS/PCs that expressed a novel Venus-luciferase fusion protein that enabled us to de... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4155192 Fri, 05 Nov 2010 00:00:00 +0100 4155192 http://www.medworm.com/index.php?rid=4155191&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054931%26dopt%3DAbstract Authors: Mizuno S Cell constructs and culture methods are essential tools in tissue engineering. The cell construct should be equivalent to the native cartilage it is intended to replace. Thus, three-dimensional cell constructs are usually composed of a high density of cells and dense extracellular matrix. However, dense constructs suffer from a lack of passive nutrient supply, gas exchange, and removal of degraded debris. We have developed a novel hydrostatic pressure/perfusion culture system that improves the quality of neo-tissues, providing an automated and affordable system for clinical applications. We have also developed a semipermeable membrane pouch that contains a fragile amorphous cell carrier. Although amorphous material is difficult to handle, it is a useful medium in whic... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4155191 Fri, 05 Nov 2010 00:00:00 +0100 4155191 http://www.medworm.com/index.php?rid=4155190&cid=s_37764_171_f&fid=37764&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D21054932%26dopt%3DAbstract Authors: Lo T, Ho JH, Yang MH, Lee OK The unique self-renewal and multi-lineage differentiation potential of mesenchymal stem cells (MSCs) make them a promising candidate for cell therapy applications. However, during in vitro expansion of MSCs, replicative senescence may occur and will compromise the quality of the expanded cells. Since calorie restriction has been shown to effectively extend the lifespan of various organisms, the purpose of this study is to investigate the effect of glucose reduction on MSCs and the coordinated changes in energy utilization. It was found that the frequency of cycling cells was significantly increased, while senescence markers such as β-galactosidase activities and p16INK4a expression level were markedly reduced in MSCs under low glucose culture cond... Cell Transplantation journals http://www.medworm.com/rss/comments.php?id=4155190 Fri, 05 Nov 2010 00:00:00 +0100 4155190