MedWorm.com provides a medical RSS filtering service. Over 6000 RSS medical sources are combined and output via different filters. This feed contains the latest items from the 'Chembiochem' source. Sat, 20 Mar 2010 16:01:14 +0100 http://www.medworm.com/index.php?rid=3379446&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20235107%26dopt%3DAbstract Authors: Wittrup Larsen M, Zielinska DF, Martinelle M, Hidalgo A, Jensen LJ, Bornscheuer UT, Hult K A water tunnel in Candida antarctica lipase B that provides the active site with substrate water is hypothesized. A small, focused library created in order to prevent water from entering the active site through the tunnel was screened for increased transacylation over hydrolysis activity. A single mutant, S47L, in which the inner part of the tunnel was blocked, catalysed the transacylation of vinyl butyrate to 20 mM butanol 14 times faster than hydrolysis. The single mutant Q46A, which has a more open outer end of the tunnel, showed an increased hydrolysis rate and a decreased hydrolysis to transacylation ratio compared to the wild-type lipase. Mutants with a blocked tunnel could be very... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3379446 Tue, 16 Mar 2010 00:00:00 +0100 3379446 http://www.medworm.com/index.php?rid=3379445&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20235108%26dopt%3DAbstract Authors: Köhler V, Ward TR PMID: 20235108 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3379445 Tue, 16 Mar 2010 00:00:00 +0100 3379445 http://www.medworm.com/index.php?rid=3379444&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20235110%26dopt%3DAbstract Authors: Ohtake N, Niikura K, Suzuki T, Nagakawa K, Mikuni S, Matsuo Y, Kinjo M, Sawa H, Ijiro K PMID: 20235110 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3379444 Tue, 16 Mar 2010 00:00:00 +0100 3379444 http://www.medworm.com/index.php?rid=3360068&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20217883%26dopt%3DAbstract Authors: Gallagher SS, Jing C, Peterka DS, Konate M, Wombacher R, Kaufman LJ, Yuste R, Cornish VW PMID: 20217883 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3360068 Tue, 09 Mar 2010 00:00:00 +0100 3360068 http://www.medworm.com/index.php?rid=3360067&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20217884%26dopt%3DAbstract Authors: Volmer AA, Carreira EM PMID: 20217884 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3360067 Tue, 09 Mar 2010 00:00:00 +0100 3360067 http://www.medworm.com/index.php?rid=3360069&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20213779%26dopt%3DAbstract Authors: Schlosser K, Li Y Enzymes play a crucial role in all living organisms by accelerating the rates of a myriad of biochemical reactions that are necessary to sustain life. Although the vast majority of known enzymes are made of protein, in recent years it has become increasingly apparent that other molecular formats, like nucleic acids, can also serve in this capacity. DNAzymes (also known as deoxyribozymes) are synthetic enzymes made of short, single strands of deoxyribonucleic acid. These DNA-based enzymes offer the prospect of significant commercial utility, because they are exceptionally stable and can be produced very easily and inexpensively. The study of one particular DNAzyme, known as "8-17", has enhanced our understanding of DNAzyme-mediated catalysis. Moreover, the fun... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3360069 Mon, 08 Mar 2010 00:00:00 +0100 3360069 http://www.medworm.com/index.php?rid=3346849&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20209557%26dopt%3DAbstract Authors: Okada S, Mizukami S, Kikuchi K PMID: 20209557 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3346849 Fri, 05 Mar 2010 00:00:00 +0100 3346849 http://www.medworm.com/index.php?rid=3346848&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20209558%26dopt%3DAbstract Authors: Weikart ND, Mootz HD PMID: 20209558 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3346848 Fri, 05 Mar 2010 00:00:00 +0100 3346848 http://www.medworm.com/index.php?rid=3346847&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20209559%26dopt%3DAbstract Authors: Kuntz DA, Nakayama S, Shea K, Hori H, Uto Y, Nagasawa H, Rose DR Golgi alpha-mannosidase II (GMII) is a key enzyme in the N-glycosylation pathway and is a potential target for cancer chemotherapy. The natural product swainsonine is a potent inhibitor of GMII. In this paper we characterize the binding of 5alpha-substituted swainsonine analogues to the soluble catalytic domain of Drosophila GMII by X-ray crystallography. These inhibitors enjoy an advantage over previously reported GMII inhibitors in that they did not significantly decrease the inhibitory potential of the swainsonine head-group. The phenyl groups of these analogues occupy a portion of the binding site not previously seen to be populated with either substrate analogues or other inhibitors and they form novel hydro... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3346847 Fri, 05 Mar 2010 00:00:00 +0100 3346847 http://www.medworm.com/index.php?rid=3346846&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20209560%26dopt%3DAbstract Authors: Liu D, Trodler P, Eiben S, Koschorreck K, Müller M, Pleiss J, Maurer SC, Branneby C, Schmid RD, Hauer B Pseudozyma antarctica lipase B (CALB) shows activity in the acrylation of hydroxypropylcarbamate, a racemic mixture of enantiomers of primary and secondary alcohols. However, full conversion is hampered by the slowly reacting S enantiomer of the secondary alcohol. The same is true for a wide range of secondary alcohols, for example, octan-2- and -3-ol. In order to get high conversion in these reactions in a short time, the stereospecificity pocket of CALB was redesigned by using predictions from molecular modeling. Positions 278, 104, and 47 were targeted, and a library for two-site saturation mutagenesis at positions 104 and 278 was constructed. The library was then sc... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3346846 Fri, 05 Mar 2010 00:00:00 +0100 3346846 http://www.medworm.com/index.php?rid=3346845&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20209562%26dopt%3DAbstract Authors: Berry AF, Heal WP, Tarafder AK, Tolmachova T, Baron RA, Seabra MC, Tate EW PMID: 20209562 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3346845 Fri, 05 Mar 2010 00:00:00 +0100 3346845 http://www.medworm.com/index.php?rid=3339896&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20198673%26dopt%3DAbstract Authors: Pulsipher A, Yousaf MN PMID: 20198673 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3339896 Tue, 02 Mar 2010 00:00:00 +0100 3339896 http://www.medworm.com/index.php?rid=3327861&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20191651%26dopt%3DAbstract Authors: Musk DJ PMID: 20191651 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3327861 Sun, 28 Feb 2010 00:00:00 +0100 3327861 http://www.medworm.com/index.php?rid=3327860&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20191652%26dopt%3DAbstract Authors: Ruth C, Glieder A Acting on the transcriptional level, synthetic promoters have been useful tools for controlling gene expression and have applications in many fields. Here, we discuss synthetic promoters and libraries in regard to current and future applications in the field of biocatalysis or biotransformation. We also focus on synthetic promoter design principles and distinguish between prokaryotic and eukaryotic destinations. The natural toolboxes available for tuneable gene expression and the regulation of enzyme function are limited and primarily host specific. Synthetic biology offers generally applicable concepts and quick implementation. Smart alternatives to transcriptional regulation enrich the engineer's tool box for optimizing industrial enzyme production and host... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3327860 Sun, 28 Feb 2010 00:00:00 +0100 3327860 http://www.medworm.com/index.php?rid=3327859&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20191656%26dopt%3DAbstract Authors: Marsh EN, Patterson DP, Li L Adenosine is undoubtedly an ancient biological molecule that is a component of many enzyme cofactors: ATP, FADH, NAD(P)H, and coenzyme A, to name but a few, and, of course, of RNA. Here we present an overview of the role of adenosine in its most reactive form: as an organic radical formed either by homolytic cleavage of adenosylcobalamin (coenzyme B(12), AdoCbl) or by single-electron reduction of S-adenosylmethionine (AdoMet) complexed to an iron-sulfur cluster. Although many of the enzymes we discuss are newly discovered, adenosine's role as a radical cofactor most likely arose very early in evolution, before the advent of photosynthesis and the production of molecular oxygen, which rapidly inactivates many radical enzymes. AdoCbl-dependent enzyme... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3327859 Sun, 28 Feb 2010 00:00:00 +0100 3327859 http://www.medworm.com/index.php?rid=3315290&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20187057%26dopt%3DAbstract Authors: Kumar Sinha D, Neveu P, Gagey N, Aujard I, Benbrahim-Bouzidi C, Le Saux T, Rampon C, Gauron C, Goetz B, Dubruille S, Baaden M, Volovitch M, Bensimon D, Vriz S, Jullien L We have implemented a noninvasive optical method for the fast control of protein activity in a live zebrafish embryo. It relies on releasing a protein fused to a modified estrogen receptor ligand binding domain from its complex with cytoplasmic chaperones, upon the local photoactivation of a nonendogenous caged inducer. Molecular dynamics simulations were used to design cyclofen-OH, a photochemically stable inducer of the receptor specific for 4-hydroxy-tamoxifen (ER(T2)). Cyclofen-OH was easily synthesized in two steps with good yields. At submicromolar concentrations, it activates proteins fused to the ER(T2... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3315290 Wed, 24 Feb 2010 00:00:00 +0100 3315290 http://www.medworm.com/index.php?rid=3315291&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20186904%26dopt%3DAbstract Authors: Goss RJ, Lanceron S, Deb Roy A, Sprague S, Nur-E-Alam M, Hughes DL, Wilkinson B, Moss SJ Rapamycin is a drug with several important clinical uses. Its complex structure means that total synthesis of this natural product and its analogues is demanding and lengthy. A more expeditious approach is to utilise biosynthesis to enable the generation of otherwise synthetically intractable analogues. In order to achieve this, rules governing biosynthetic precursor substrate preference must be established. Through determining these rules and synthesising and administering suitable substrate precursors, we demonstrate the first generation of fluorinated rapamycin analogues. Here we report the generation of six new fluororapamycins. PMID: 20186904 [PubMed - as supplied by publisher] (S... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3315291 Tue, 23 Feb 2010 00:00:00 +0100 3315291 http://www.medworm.com/index.php?rid=3308384&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20183841%26dopt%3DAbstract Authors: Zehentgruber D, Hannemann F, Bleif S, Bernhardt R, Lütz S Cytochrome P450 monooxygenases are of outstanding interest for the synthesis of pharmaceuticals and fine chemicals, due to their ability to hydroxylate C--H bonds mainly in a stereo- and regioselective manner. CYP106A2 from Bacillus megaterium ATCC 13368, one of only a few known bacterial steroid hydroxylases, enables the oxidation of 3-keto-4-ene steroids mainly at position 15. We expressed this enzyme together with the electron-transfer partners bovine adrenodoxin and adrenodoxin reductase in Escherichia coli. Additionally an enzyme-coupled cofactor regeneration system was implemented by expressing alcohol dehydrogenase from Lactobacillus brevis. By studying the conversion of progesterone and testosterone, the bo... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3308384 Mon, 22 Feb 2010 00:00:00 +0100 3308384 http://www.medworm.com/index.php?rid=3308383&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20183842%26dopt%3DAbstract Authors: Förster U, Lommel K, Sauter D, Grünewald C, Engels JW, Wachtveitl J A series of short RNA duplexes containing one or two 1-ethynylpyrene-modified adenine bases was synthesised. The melting behaviour of these duplexes was examined by monitoring temperature-dependent pyrene fluorescence. In the singly modified RNA duplexes, the bases flanking the ethynylpyrene-rA were varied to examine the sequence specificity of the fluorescence change of pyrene upon RNA hybridisation. Because an increase in pyrene fluorescence upon melting of the duplex can be correlated with intercalation of pyrene, and a decrease is usually associated with the position of pyrene outside the strand, a relationship between the flanking bases and the tendency of the dye to intercalate has been establi... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3308383 Mon, 22 Feb 2010 00:00:00 +0100 3308383 http://www.medworm.com/index.php?rid=3308382&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20183843%26dopt%3DAbstract Authors: Solanki A, Lee KB PMID: 20183843 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3308382 Mon, 22 Feb 2010 00:00:00 +0100 3308382 http://www.medworm.com/index.php?rid=3302032&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20175073%26dopt%3DAbstract Authors: Kwon SJ, Kim MI, Ku B, Coulombel L, Kim JH, Shawky JH, Linhardt RJ, Dordick JS PMID: 20175073 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3302032 Fri, 19 Feb 2010 00:00:00 +0100 3302032 http://www.medworm.com/index.php?rid=3302031&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20175074%26dopt%3DAbstract Authors: PMID: 20175074 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3302031 Fri, 19 Feb 2010 00:00:00 +0100 3302031 http://www.medworm.com/index.php?rid=3302030&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20175075%26dopt%3DAbstract Authors: PMID: 20175075 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3302030 Fri, 19 Feb 2010 00:00:00 +0100 3302030 http://www.medworm.com/index.php?rid=3302029&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20175182%26dopt%3DAbstract Authors: Hori Y, Egashira Y, Kamiura R, Kikuchi K PMID: 20175182 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3302029 Fri, 19 Feb 2010 00:00:00 +0100 3302029 http://www.medworm.com/index.php?rid=3284362&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20162658%26dopt%3DAbstract Authors: Hartig JS PMID: 20162658 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3284362 Tue, 16 Feb 2010 00:00:00 +0100 3284362 http://www.medworm.com/index.php?rid=3284365&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20157910%26dopt%3DAbstract Authors: Zhu L, Tee KL, Roccatano D, Sonmez B, Ni Y, Sun ZH, Schwaneberg U Arginine deiminase (ADI; EC 3.5.3.6) has been studied as a potential antitumor drug for the treatment of arginine-auxotrophic tumors, such as hepatocellular carcinomas (HCCs) and melanomas. Studies with human lymphatic leukemia cell lines confirmed that ADI is an antiangiogenic agent for treating leukemia. The main limitation of ADI from Pseudomonas plecoglossicida (PpADI) lies in its pH-dependent activity profile, its pH optimum is at 6.5. A pH shift from 6.5 to 7.5 results in an approximately 80 % drop in activity. (The pH of human plasma is 7.35 to 7.45.) In order to shift the PpADI pH optimum, a directed-evolution protocol based on an adapted citrulline-screening protocol in microtiter-plate format was devel... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3284365 Mon, 15 Feb 2010 00:00:00 +0100 3284365 http://www.medworm.com/index.php?rid=3284364&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20157911%26dopt%3DAbstract Authors: Gottschaldt M, Schubert US, Rau S, Yano S, Vos JG, Kroll T, Clement J, Hilger I PMID: 20157911 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3284364 Mon, 15 Feb 2010 00:00:00 +0100 3284364 http://www.medworm.com/index.php?rid=3284363&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20157912%26dopt%3DAbstract Authors: Zheng JS, Cui HK, Fang GM, Xi WX, Liu L PMID: 20157912 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3284363 Mon, 15 Feb 2010 00:00:00 +0100 3284363 http://www.medworm.com/index.php?rid=3267646&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20148427%26dopt%3DAbstract Authors: Usai I, Krueger M, Einsiedel J, Hillen W, Gmeiner P A click-chemistry-based synthesis of biologically active doxycycline-amino acid conjugates is described. Starting from 9-aminodoxycycline derivatives and complementary functionalized amino acids, ligation was accomplished by copper(I)-catalyzed azide-alkyne [3+2] cycloaddition (CuAAC). The final products were tested in a variety of TetR and revTetR systems, and the C-terminally linked phenylalanine conjugate 12 c exhibited high selectivity for revTetR over TetR. Besides the unique property of the specific effector 12 c to effectively differentiate TetR and its reverse phenotype, the test compound proved to be almost devoid of any antibacterial activity; this will be highly beneficial for future applications to control gene ex... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3267646 Wed, 10 Feb 2010 00:00:00 +0100 3267646 http://www.medworm.com/index.php?rid=3267645&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20148428%26dopt%3DAbstract Authors: Schneider S, Gutiérrez M, Sandalova T, Schneider G, Clapés P, Sprenger GA, Samland AK Recently, we reported on a transaldolase B variant (TalB F178Y) that is able to use dihydroxyacetone (DHA) as donor in aldol reactions. In a second round of protein engineering, we aimed at improving the affinity of this variant towards nonphosphorylated acceptor aldehydes, that is, glyceraldehyde (GA). The anion binding site was identified in the X-ray structure of TalB F178Y where a sulfate ion from the buffer was bound in the active site. Therefore, we performed site-directed saturation mutagenesis at three residues forming the putative phosphate binding site, Arg181, Ser226 and Arg228. The focused libraries were screened for the formation of D-fructose from DHA and d,l-GA by usi... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3267645 Wed, 10 Feb 2010 00:00:00 +0100 3267645 http://www.medworm.com/index.php?rid=3260885&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20146276%26dopt%3DAbstract Authors: Nakamura R, Okamoto A, Tajima N, Newton GJ, Kai F, Takashima T, Hashimoto K PMID: 20146276 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3260885 Tue, 09 Feb 2010 00:00:00 +0100 3260885 http://www.medworm.com/index.php?rid=3260887&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20143366%26dopt%3DAbstract Authors: Varma Y, Hendrickson T PMID: 20143366 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3260887 Mon, 08 Feb 2010 00:00:00 +0100 3260887 http://www.medworm.com/index.php?rid=3260886&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20143368%26dopt%3DAbstract Authors: Ottmann C, Hauske P, Kaiser M PMID: 20143368 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3260886 Mon, 08 Feb 2010 00:00:00 +0100 3260886 http://www.medworm.com/index.php?rid=3254712&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140893%26dopt%3DAbstract Authors: Nätscher JB, Laskowski N, Kraft P, Tacke R PMID: 20140893 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3254712 Fri, 05 Feb 2010 00:00:00 +0100 3254712 http://www.medworm.com/index.php?rid=3254711&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140894%26dopt%3DAbstract Authors: Goelitz P PMID: 20140894 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3254711 Fri, 05 Feb 2010 00:00:00 +0100 3254711 http://www.medworm.com/index.php?rid=3254710&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140895%26dopt%3DAbstract Authors: PMID: 20140895 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3254710 Fri, 05 Feb 2010 00:00:00 +0100 3254710 http://www.medworm.com/index.php?rid=3254709&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140896%26dopt%3DAbstract Authors: PMID: 20140896 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3254709 Fri, 05 Feb 2010 00:00:00 +0100 3254709 http://www.medworm.com/index.php?rid=3254708&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20140934%26dopt%3DAbstract Authors: Kharel MK, Nybo SE, Shepherd MD, Rohr J The gene clusters responsible for the biosynthesis of two antitumor antibiotics, ravidomycin and chrysomycin, have been cloned from Streptomyces ravidus and Streptomyces albaduncus, respectively. Sequencing of the 33.28 kb DNA region of the cosmid cosRav32 and the 34.65 kb DNA region of cosChry1-1 and cosChryF2 revealed 36 and 35 open reading frames (ORFs), respectively, harboring tandem sets of type II polyketide synthase (PKS) genes, D-ravidosamine and D-virenose biosynthetic genes, post-PKS tailoring genes, regulatory genes, and genes of unknown function. The isolated ravidomycin gene cluster was confirmed to be involved in ravidomycin biosynthesis through the production of a new analogue of ravidomycin along with anticipated pathway ... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3254708 Fri, 05 Feb 2010 00:00:00 +0100 3254708 http://www.medworm.com/index.php?rid=3248806&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20135663%26dopt%3DAbstract Authors: Wachowius F, Höbartner C PMID: 20135663 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3248806 Thu, 04 Feb 2010 00:00:00 +0100 3248806 http://www.medworm.com/index.php?rid=3248805&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20135665%26dopt%3DAbstract Authors: Tosin M, Betancor L, Stephens E, Ariel Li WM, Spencer JB, Leadlay PF Modular biocatalysis is responsible for the generation of countless bioactive products and its mining remains a major focus for drug discovery purposes. One of the enduring hurdles is the isolation of biosynthetic intermediates in a readily-analysed form. We prepared a series of nonhydrolysable pantetheine and N-acetyl cysteamine mimics of the natural (methyl)malonyl extender units recruited for polyketide formation. Using these analogues as competitive substrates, we were able to trap and off-load diketide and triketide species directly from an in vitro reconstituted type I polyketide synthase, the 6-deoxyerythronolide B synthase 3 (DEBS3). The putative intermediates, which were extracted in organic solvent ... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3248805 Thu, 04 Feb 2010 00:00:00 +0100 3248805 http://www.medworm.com/index.php?rid=3244399&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127927%26dopt%3DAbstract Authors: Carlson JC, Fortman JL, Anzai Y, Li S, Burr DA, Sherman DH The structurally intriguing bicyclic ketal moiety of tirandamycin is common to several acyl-tetramic acid antibiotics, and is a key determinant of biological activity. We have identified the tirandamycin biosynthetic gene cluster from the environmental marine isolate Streptomyces sp. 307-9, thus providing the first genetic insight into the biosynthesis of this natural product scaffold. Sequence analysis revealed a hybrid polyketide synthase-nonribosomal peptide synthetase gene cluster with a colinear domain organization, which is entirely consistent with the core structure of the tirandamycins. We also identified genes within the cluster that encode candidate tailoring enzymes for elaboration and modification of the bi... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3244399 Tue, 02 Feb 2010 00:00:00 +0100 3244399 http://www.medworm.com/index.php?rid=3244398&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127928%26dopt%3DAbstract Authors: Weissman KJ PMID: 20127928 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3244398 Tue, 02 Feb 2010 00:00:00 +0100 3244398 http://www.medworm.com/index.php?rid=3244401&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127780%26dopt%3DAbstract Authors: Selvakumar E, Rameshkumar N, Lee SG, Lee SJ, Park HS PMID: 20127780 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3244401 Mon, 01 Feb 2010 00:00:00 +0100 3244401 http://www.medworm.com/index.php?rid=3244400&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20127782%26dopt%3DAbstract Authors: Udit AK, Hackenberger CP, O'Reilly MK PMID: 20127782 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3244400 Mon, 01 Feb 2010 00:00:00 +0100 3244400 http://www.medworm.com/index.php?rid=3223832&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20112320%26dopt%3DAbstract Authors: Leitgeb S, Nidetzky B PMID: 20112320 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3223832 Thu, 28 Jan 2010 00:00:00 +0100 3223832 http://www.medworm.com/index.php?rid=3223831&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20112321%26dopt%3DAbstract Authors: Armstrong Z, Reitinger S, Kantner T, Withers SG Thioglycoligases are engineered enzymes for the synthesis of thioglycosides that are derived from retaining glycosidases by replacing the acid/base catalyst. The optimal choice of substitution for the acid/base mutant is currently unknown, so to investigate this question a complete acid/base library of the model glycosidase Bacillus circulans xylanase (Bcx) was generated by using site-saturation mutagenesis. A novel screening approach combining active site titration with semiquantitative product analysis by thin layer chromatography was established and used to evaluate specific activities of each mutant enzyme within crude cell lysates. The six most active Bcx variants were analyzed in more detail, a pH optimum of 8.5 was establi... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3223831 Thu, 28 Jan 2010 00:00:00 +0100 3223831 http://www.medworm.com/index.php?rid=3223830&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20112322%26dopt%3DAbstract Authors: Yin BC, Zhang M, Tan W, Ye BC PMID: 20112322 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3223830 Thu, 28 Jan 2010 00:00:00 +0100 3223830 http://www.medworm.com/index.php?rid=3211176&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099290%26dopt%3DAbstract Authors: Lange V, Becker-Baldus J, Kunert B, van Rossum BJ, Casagrande F, Engel A, Roske Y, Scheffel FM, Schneider E, Oschkinat H ATP-binding cassette (ABC) transport systems facilitate the translocation of substances, like amino acids, across cell membranes energised by ATP hydrolysis. This work describes first structural studies on the ABC transporter ArtMP from Geobacillus stearothermophilus in native lipid environment by magic-angle spinning NMR spectroscopy. The 2D crystals of ArtMP and 3D crystals of isolated ArtP were prepared in different nucleotide-bound or -unbound states. From selectively (13)C,(15)N-labelled ArtP, several sequence-specific assignments were obtained, most of which could be transferred to spectra of ArtMP. Residues Tyr133 and Pro134 protrude directly into the... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3211176 Fri, 22 Jan 2010 00:00:00 +0100 3211176 http://www.medworm.com/index.php?rid=3211175&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20099291%26dopt%3DAbstract Authors: Van Engelenburg SB, Nahreini T, Palmer AE PMID: 20099291 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3211175 Fri, 22 Jan 2010 00:00:00 +0100 3211175 http://www.medworm.com/index.php?rid=3200860&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20095001%26dopt%3DAbstract Authors: Hopkinson RJ, Hamed RB, Rose NR, Claridge TD, Schofield CJ PMID: 20095001 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3200860 Thu, 21 Jan 2010 00:00:00 +0100 3200860 http://www.medworm.com/index.php?rid=3200861&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20091728%26dopt%3DAbstract Authors: Gallardo R, Ivarsson Y, Schymkowitz J, Rousseau F, Zimmermann P PDZ domains are globular protein modules that are over-and-above appreciated for their interaction with short peptide motifs found in the cytosolic tail of membrane receptors, channels, and adhesion molecules. These domains predominate in scaffold molecules that control the assembly and the location of large signaling complexes. Studies have now emerged showing that PDZ domains can also interact with membrane lipids, and in particular with phosphoinositides. Phosphoinositides control various aspects of cell signaling, vesicular trafficking, and cytoskeleton remodeling. When investigated, lipid binding appears to be extremely relevant for PDZ protein functionality. Studies point to more than one mechanism for PDZ d... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3200861 Wed, 20 Jan 2010 00:00:00 +0100 3200861 http://www.medworm.com/index.php?rid=3194389&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20087976%26dopt%3DAbstract Authors: Olsen CA PMID: 20087976 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3194389 Wed, 20 Jan 2010 00:00:00 +0100 3194389 http://www.medworm.com/index.php?rid=3194388&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20087977%26dopt%3DAbstract Authors: PMID: 20087977 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3194388 Wed, 20 Jan 2010 00:00:00 +0100 3194388 http://www.medworm.com/index.php?rid=3194387&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20087978%26dopt%3DAbstract Authors: PMID: 20087978 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3194387 Wed, 20 Jan 2010 00:00:00 +0100 3194387 http://www.medworm.com/index.php?rid=3186138&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20082398%26dopt%3DAbstract Authors: Gareiss PC, Schneekloth AR, Salcius MJ, Seo SY, Crews CM The development of new ligands for the oncoprotein Ras can provide tools for the study of this important signaling component or potentially serve as therapeutic agents for the treatment of Ras-associated diseases. Herein, we report a peptidic Ras ligand identified through naïve phage display. Panning a phage library with a diversity of 10(9) transormants successfully identified a peptide dodecamer that contains two internal consensus motifs and binds Ras in both the active GTP- and inactive GDP-bound conformations with low micromolar dissociation constants. The dodecamer does not alter the intrinsic GTPase activity of Ras, does not compete for Ras binding to the Ras binding domain of Raf, and does not alter cell via... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3186138 Fri, 15 Jan 2010 00:00:00 +0100 3186138 http://www.medworm.com/index.php?rid=3186137&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20082400%26dopt%3DAbstract Authors: Shinozaki J, Shibuya M, Takahata Y, Masuda K, Ebizuka Y Triterpenes, a diverse group of natural products comprising six isoprene units, are distributed across various organisms from bacteria to higher plants. Ferns are sporophytes that produce triterpenes and are lower on the evolutionary scale than higher plants. Among ferns that produce triterpenes analogous to bacterial hopanoids, Polypodiodes niponica produces migrated dammaranes and oleananes, which are also widely found in higher plants. Because the study of terpene-producing ferns could help us to understand the molecular basis of triterpene biosynthesis, cDNA cloning of squalene cyclases (SCs) from P. niponica was carried out. Two SCs (PNT and PNG) were obtained. The heterologously expressed PNT produces tirucalla-7,21... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3186137 Fri, 15 Jan 2010 00:00:00 +0100 3186137 http://www.medworm.com/index.php?rid=3176714&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20077457%26dopt%3DAbstract Authors: Richards JL, Seward GK, Wang YH, Dmochowski IJ PMID: 20077457 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3176714 Wed, 13 Jan 2010 00:00:00 +0100 3176714 http://www.medworm.com/index.php?rid=3169616&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20069621%26dopt%3DAbstract We present a method for the automated extraction and classification of ligand binding site topologies, in which protein surface cavities are represented as branched frameworks. The procedure employs a growing neural gas approach for pocket topology assignment and pocket framework generation. We assessed the structural diversity of 623 known ligand binding site topologies based on framework cluster analysis. At a resolution of 5 A only 23 structurally distinct topology groups were formed; this suggests an overall limited structural diversity of ligand-accommodating protein cavities. Higher resolution allowed for identification of protein-family specific pocket features. Pocket frameworks highlight potentially preferred modes of ligand-receptor interactions and will help facilitate the ident... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3169616 Tue, 12 Jan 2010 00:00:00 +0100 3169616 http://www.medworm.com/index.php?rid=3164003&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20063337%26dopt%3DAbstract Authors: Georgianna WE, Deiters A PMID: 20063337 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3164003 Fri, 08 Jan 2010 00:00:00 +0100 3164003 http://www.medworm.com/index.php?rid=3157773&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20058251%26dopt%3DAbstract Authors: Tsuji A, Yoshikawa K We constructed a phospholipid-coated water-in-oil microdroplet (20-60 mum in diameter) that encapsulated plasmid DNA containing a human beta-actin cDNA sequence, fluorescent oligonucleotide probes, and other components of the transcription reaction. Transcription inside individual microdroplets was investigated in real time by using fluorescence microscopy. The progress of the transcription reaction was successively monitored by fluorescence resonance energy transfer (FRET), which was derived from the specific hybridization of fluorescent oligonucleotide probes to the beta-actin mRNAs synthesized. In microdroplets composed of phosphatidylethanolamine, DNAs were located in the aqueous phase or on the membrane surface depending on the Mg(II) concentration. F... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3157773 Thu, 07 Jan 2010 00:00:00 +0100 3157773 http://www.medworm.com/index.php?rid=3157772&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20058252%26dopt%3DAbstract Authors: Merkel L, Hoesl MG, Albrecht M, Schmidt A, Budisa N In vivo expression of colored proteins without post-translational modification or chemical functionalization is highly desired for protein studies and cell biology. Cell-permeable tryptophan analogues, such as azatryptophans, have proved to be almost ideal isosteric substitutes for natural tryptophan in cellular proteins. Their unique spectral features, such as markedly red-shifted fluorescence, are transmitted into protein structures upon incorporation. Among the azaindoles under study (2-, 4-, 5-, 6-, and 7-azaindole) 4-azaindole has exhibited the largest Stokes shift (~130 nm) in steady-state fluorescence measurements. It is also highly biocompatible and as 4-azatryptophan it can be translated into target protein sequences... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3157772 Thu, 07 Jan 2010 00:00:00 +0100 3157772 http://www.medworm.com/index.php?rid=3157771&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20058253%26dopt%3DAbstract Authors: Kwon SJ, Kim MI, Ku B, Coulombel L, Kim JH, Shawky JH, Linhardt RJ, Dordick JS Receptor tyrosine kinases are critical targets for the regulation of cell survival. Cancer patients with abnormal receptor tyrosine kinases (RTK) tend to have more aggressive disease with poor clinical outcomes. As a result, human epidermal growth factor receptor kinases, such as EGFR (HER1), HER2, and HER3, represent important therapeutic targets. Several plant polyphenols including the type III polyketide synthase products (genistein, curcumin, resveratrol, and epigallocatechin-3-galate) possess chemopreventive activity, primarily as a result of RTK inhibition. However, only a small fraction of the polyphenolic structural universe has been evaluated. Along these lines, we have developed an in vitr... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3157771 Thu, 07 Jan 2010 00:00:00 +0100 3157771 http://www.medworm.com/index.php?rid=3157770&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20058254%26dopt%3DAbstract Authors: Nätscher JB, Laskowski N, Kraft P, Tacke R PMID: 20058254 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3157770 Thu, 07 Jan 2010 00:00:00 +0100 3157770 http://www.medworm.com/index.php?rid=3157769&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20058255%26dopt%3DAbstract This study shows that three important factors govern potent inhibition: 1) the surface area sequestered by the assembled ligands; 2) the spacing between ligand modules since a longer distance is required to target DM2 RNAs than DM1 RNAs; and 3) flexibility in the modular assembly scaffold used to display the RNA-binding module. These results have impacts on the general design of assembled ligands targeting RNAs present in genomic sequence. PMID: 20058255 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3157769 Thu, 07 Jan 2010 00:00:00 +0100 3157769 http://www.medworm.com/index.php?rid=3146638&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20049756%26dopt%3DAbstract Authors: Dalhoff C, Hüben M, Lenz T, Poot P, Nordhoff E, Köster H, Weinhold E Understanding the interplay of different cellular proteins and their substrates is of major interest in the postgenomic era. For this purpose, selective isolation and identification of proteins from complex biological samples is necessary and targeted isolation of enzyme families is a challenging task. Over the last years, methods like activity-based protein profiling (ABPP) and capture compound mass spectrometry (CCMS) have been developed to reduce the complexity of the proteome by means of protein function in contrast to standard approaches, which utilize differences in physical properties for protein separation. To isolate and identify the subproteome consisting of S-adenosyl-L-methionine (SAM or... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3146638 Mon, 04 Jan 2010 00:00:00 +0100 3146638 http://www.medworm.com/index.php?rid=3146637&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20049758%26dopt%3DAbstract Authors: Rogers SA, Bero JD, Melander C A collection of 2-aminoimidazole/triazole amides has been synthesized and screened for antibiofilm activity. This class of small molecules was found to modulate the biofilm activity of Pseudomonas aeruginosa, a multidrug-resistant strain of Acinetobacter baumannii (MDRAB), a methicillin-resistant Staphylococcus aureus strain (MRSA), Escherichia coli, Rhodospirillum salexigens, Staphylococcus epidermidis, Vibrio vulnificus, and vancomycin-resistant Enterococcus faecium as well as the yeast Candida albicans and Cryptococcus neoformans. Furthermore, lead compounds were found to not lyse red blood cells at active concentrations. PMID: 20049758 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3146637 Mon, 04 Jan 2010 00:00:00 +0100 3146637 http://www.medworm.com/index.php?rid=3146636&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20049759%26dopt%3DAbstract Authors: Vallin M, Syrén PO, Hult K The size of the stereoselectivity pocket of Candida antarctica lipase B limits the range of alcohols that can be resolved with this enzyme. These steric constrains have been changed by increasing the size of the pocket by the mutation W104A. The mutated enzyme has good activity and enantioselectivity toward bulky secondary alcohols, such as 1-phenylalkanols, with alkyl chains up to eight carbon atoms. The S enantiomer was preferred in contrast to the wild-type enzyme, which has R selectivity. The magnitude of the enantioselectivity changes in an interesting way with the chain length of the alkyl moiety. It is governed by interplay between entropic and enthalpic contributions and substrates with long alkyl chains are resolved best with E values h... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3146636 Mon, 04 Jan 2010 00:00:00 +0100 3146636 http://www.medworm.com/index.php?rid=3146635&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20049760%26dopt%3DAbstract Authors: Pedersen SL, Steentoft C, Vrang N, Jensen KJ The increasing prevalence of obesity worldwide calls for safe and highly efficacious satiety drugs. PYY3-36 has been implicated in food intake regulation, and novel peptide analogues with high Y2 receptor-subtype selectivity and potency have potential as drugs for the treatment of obesity. It has been hypothesized that PYY3-36 associates with the plasma membrane prior to receptor activation such that the amphipathic alpha-helix of PYY3-36 possibly guides the C-terminal pentapeptide into the correct conformation for receptor activation. Ala-scans are used routinely to study the effect of individual amino acids in a given peptide sequence. Here we report the glyco-scan of the peptide hormone PYY3-36, in which hydroxyl side-chain funct... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3146635 Mon, 04 Jan 2010 00:00:00 +0100 3146635 http://www.medworm.com/index.php?rid=3146634&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20049761%26dopt%3DAbstract Authors: Cinque L, Ghomchi Y, Chen Y, Bensimon A, Baigl D PMID: 20049761 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3146634 Mon, 04 Jan 2010 00:00:00 +0100 3146634 http://www.medworm.com/index.php?rid=3134875&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043306%26dopt%3DAbstract Authors: Funabashi M, Baba S, Nonaka K, Hosobuchi M, Fujita Y, Shibata T, Van Lanen SG PMID: 20043306 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3134875 Wed, 30 Dec 2009 00:00:00 +0100 3134875 http://www.medworm.com/index.php?rid=3134874&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043307%26dopt%3DAbstract Authors: Weinrich D, Köhn M, Jonkheijm P, Westerlind U, Dehmelt L, Engelkamp H, Christianen PC, Kuhlmann J, Maan JC, Nüsse D, Schröder H, Wacker R, Voges E, Breinbauer R, Kunz H, Niemeyer CM, Waldmann H A mild, fast and flexible method for photoimmobilization of biomolecules based on the light-initiated thiol-ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, oligonucleotides, peptides, and MUC1 tandem repeat glycopeptides were prepared with this photoimmobilization method. Furthermore, MUC1 tandem repeat glycopeptide microarrays were successfully used to probe antibodies in mouse serum obtained from vaccinated mice. Dimensions of biomo... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3134874 Wed, 30 Dec 2009 00:00:00 +0100 3134874 http://www.medworm.com/index.php?rid=3134873&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043308%26dopt%3DAbstract Authors: Dickschat JS, Zell C, Brock NL The volatiles released by Phaeobacter gallaeciensis, Oceanibulbus indolifex and Dinoroseobacter shibae have been investigated by GC-MS, and several MeSH-derived sulfur volatiles have been identified. An important sulfur source in the oceans is the algal metabolite dimethylsulfoniopropionate (DMSP). Labelled [(2)H(6)]DMSP was fed to the bacteria to investigate the production of volatiles from this compound through the lysis pathway to [(2)H(6)]dimethylsulfide or the demethylation pathway to [(2)H(3)]-3-(methylmercapto)propionic acid and lysis to [(2)H(3)]MeSH. [(2)H(6)]DMSP was efficiently converted to [(2)H(3)]MeSH by all three species. Several DMSP derivatives were synthesised and used in feeding experiments. Strong dealkylation activity was obs... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3134873 Wed, 30 Dec 2009 00:00:00 +0100 3134873 http://www.medworm.com/index.php?rid=3134872&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20043309%26dopt%3DAbstract Authors: Weiss IM PMID: 20043309 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3134872 Wed, 30 Dec 2009 00:00:00 +0100 3134872 http://www.medworm.com/index.php?rid=3129865&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20039251%26dopt%3DAbstract Authors: Berkessel A, Seelig B, Schwengberg S, Hescheler J, Sachinidis A A transgenic murine embryonic stem (ES) cell lineage expressing enhanced green fluorescent protein (EGFP) under the control of alpha-myosine heavy chain (alpha-MHC) promoter (palpha-MHC-EGFP) was used to investigate the effects of (thio)urea and cinchona alkaloid derivatives on cardiomyogenesis. The screening of the compounds yielded cardiomyogenesis inducing substances with good (IV-5, V-4) to very good activities (II-16, IV-8), as determined by a 50 to 80 % increase in the EGFP fluorescence compared to untreated cells. Time-dependent screening approaches in which compounds were added at different developmental stages of the ES cells appeared to be of limited suitability for the identification of potential cellul... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3129865 Mon, 28 Dec 2009 00:00:00 +0100 3129865 http://www.medworm.com/index.php?rid=3129864&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20039252%26dopt%3DAbstract Authors: Hagan WJ Progress toward a protometabolism (the earliest energy storage networks) has been severely hindered by a shortage of driver reactions, which could have harnessed solar photons or coupled electron sources/sinks on the primordial Earth. Here, it is reported for the first time that thioacetate can be converted into a known metabolite, acetyl phosphate, by ultraviolet light and in aqueous solution at neutral pH. Of more compelling importance, the synthesis is catalyzed by uracil, which suggests that a genetic component may have also facilitated the emergence of metabolic pathways. The chemistry of acetyl phosphate has been extensively studied, and it is known to be a precursor of phosphate esters, pyrophosphate and possibly longer inorganic chains. Moreover, its bifunctio... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3129864 Mon, 28 Dec 2009 00:00:00 +0100 3129864 http://www.medworm.com/index.php?rid=3129863&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20039253%26dopt%3DAbstract Authors: Kaysser L, Siebenberg S, Kammerer B, Gust B PMID: 20039253 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3129863 Mon, 28 Dec 2009 00:00:00 +0100 3129863 http://www.medworm.com/index.php?rid=3129862&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20039254%26dopt%3DAbstract Authors: Rezaei Araghi R, Jäckel C, Cölfen H, Salwiczek M, Völkel A, Wagner SC, Wieczorek S, Baldauf C, Koksch B PMID: 20039254 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3129862 Mon, 28 Dec 2009 00:00:00 +0100 3129862 http://www.medworm.com/index.php?rid=3122506&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20034118%26dopt%3DAbstract Authors: PMID: 20034118 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3122506 Wed, 23 Dec 2009 00:00:00 +0100 3122506 http://www.medworm.com/index.php?rid=3122505&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20034119%26dopt%3DAbstract Authors: PMID: 20034119 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3122505 Wed, 23 Dec 2009 00:00:00 +0100 3122505 http://www.medworm.com/index.php?rid=3122504&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20034120%26dopt%3DAbstract Authors: Mothes C, Larregola M, Quancard J, Goasdoué N, Lavielle S, Chassaing G, Lequin O, Karoyan P PMID: 20034120 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3122504 Wed, 23 Dec 2009 00:00:00 +0100 3122504 http://www.medworm.com/index.php?rid=3119322&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20029930%26dopt%3DAbstract Authors: Lee YJ, Erazo-Oliveras A, Pellois JP PMID: 20029930 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3119322 Tue, 22 Dec 2009 00:00:00 +0100 3119322 http://www.medworm.com/index.php?rid=3104081&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20017179%26dopt%3DAbstract Authors: Olsen CA Peptidomimetic oligomers and foldamers have received considerable attention for over a decade, with beta-peptides and the so-called peptoids (N-alkylglycine oligomers) representing prominent examples of such architectures. Lately, hybrid or mixed backbones consisting of both alpha- and beta-amino acids (alpha/beta-peptides) have been investigated in some detail as well. The present Minireview is a survey of the literature concerning hybrid structures of alpha-amino acids and peptoids, including beta-peptoids (N-alkyl-beta-alanine oligomers), and is intended to give an overview of this area of research within the field of peptidomimetic science. PMID: 20017179 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3104081 Wed, 16 Dec 2009 00:00:00 +0100 3104081 http://www.medworm.com/index.php?rid=3104083&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20014270%26dopt%3DAbstract Authors: Boán F, Gómez-Márquez J PMID: 20014270 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3104083 Mon, 14 Dec 2009 00:00:00 +0100 3104083 http://www.medworm.com/index.php?rid=3104082&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20014271%26dopt%3DAbstract Authors: Uhlich NA, Natalello A, Kadam RU, Doglia SM, Reymond JL, Darbre T The third-generation peptide-dendrimer B1 (AcES)(8)(BEA)(4)(K-Amb-Y)(2)BCD-NH(2) (B=branching (S)-2,3-diaminopropanoic acid, K=branching lysine, Amb=4-aminomethyl-benzoic acid) is the first synthetic model for cobalamin-binding proteins and binds cobalamin strongly (K(a)=5.0x10(6) M(-1)) and rapidly (k(2)=346 M(-1) s(-1)) by coordination of cobalt to the cysteine residue at the dendrimer core. A structure-activity relationship study is reported concerning the role of negative charges in binding. Substituting glutamates (E) for glutamines (Q) in the outer branches of B1 to form N3 (AcQS)(8)(BQA)(4)(B-Amb-Y)(2)BCD-NH(2) leads to stronger (K(a)=12.0x10(6) M(-1)) but slower (k(2)=67 M(-1) s(-1)) cobalamin binding. C... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3104082 Mon, 14 Dec 2009 00:00:00 +0100 3104082 http://www.medworm.com/index.php?rid=3104085&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20014085%26dopt%3DAbstract Authors: Gao X, Zhang J PMID: 20014085 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3104085 Fri, 11 Dec 2009 00:00:00 +0100 3104085 http://www.medworm.com/index.php?rid=3104084&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20014086%26dopt%3DAbstract Authors: Kunckley B, Causey CP, Pellechia PJ, Cook PF, Thompson PR Dysregulated protein arginine deiminase (PAD) activity, particularly PAD4, has been suggested to play a role in the onset and progression of numerous human diseases, including rheumatoid arthritis (RA). Given the potential role of PAD4 in RA, we set out to develop inhibitors/inactivators that could be used to modulate PAD activity and disease progression. This effort led to the discovery of two mechanism-based inactivators, denoted F- and Cl-amidine, that inactivate PAD4 by the covalent modification of an active-site cysteine that is critical for catalysis. To gain further insights into the mechanism of inactivation by these compounds, the effect of pH on the rates of inactivation was determined. These results, combined... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3104084 Fri, 11 Dec 2009 00:00:00 +0100 3104084 http://www.medworm.com/index.php?rid=3104137&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20013980%26dopt%3DAbstract Authors: Xin M, Bugg TD Substituted 2-tropolone natural products are found in plants and fungi. Their biosynthesis is thought to occur by ring expansion from a cyclohexadienone precursor, but this reaction has not previously been demonstrated experimentally. Treatment of 6-hydroxy-6-hydroxymethylcyclohexa-2,4-dienone with the non-haem iron(II)-dependent extradiol catechol dioxygenase MhpB from Escherichia coli results in the formation of the 2-tropolone ring-expansion product through a pinacol-type rearrangement. Three further substituted cyclohexa-2,4-dienone analogues were prepared, and treatment of each analogue was found to give the substituted 2-tropolone ring-expansion product. This ring expansion could also be effected nonenzymatically by treatment with 1,4,7-triazacyclononane a... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3104137 Thu, 10 Dec 2009 00:00:00 +0100 3104137 http://www.medworm.com/index.php?rid=3104104&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20013981%26dopt%3DAbstract Authors: Shaklee PM, Semrau S, Malkus M, Kubick S, Dogterom M, Schmidt T PMID: 20013981 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3104104 Thu, 10 Dec 2009 00:00:00 +0100 3104104 http://www.medworm.com/index.php?rid=3104094&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D20013982%26dopt%3DAbstract Authors: Shields JA, Rahman AS, Arthur CJ, Crosby J, Hothersall J, Simpson TJ, Thomas CM Acyl carrier proteins are vital for the biosynthesis of fatty acids and polyketides. The mupirocin biosynthetic cluster of Pseudomonas fluorescens encodes eleven type I ACPs embedded in its multifunctional polyketide synthase (PKS) proteins plus five predicted type II ACPs (mAcpA-E) that are known to be essential for mupirocin biosynthesis by deletion and complementation analysis. MupN is a putative Sfp-type phosphopantetheinyl transferase. Overexpression of three type I and three type II mupirocin ACPs in Escherichia coli, with or without mupN, followed by mass spectroscopy revealed that MupN can modify both mupirocin type I and type II ACPs to their holo-form. The endogenous phosphopantetheinyl t... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3104094 Thu, 10 Dec 2009 00:00:00 +0100 3104094 http://www.medworm.com/index.php?rid=3080058&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19998304%26dopt%3DAbstract Authors: Böhm R, Freiberger F, Stummeyer K, Gerardy-Schahn R, von Itzstein M, Haselhorst T PMID: 19998304 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3080058 Tue, 08 Dec 2009 00:00:00 +0100 3080058 http://www.medworm.com/index.php?rid=3080057&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19998400%26dopt%3DAbstract Authors: Mulyani S, Egel E, Kittel C, Turkanovic S, Wohlleben W, Süssmuth RD, van Pée KH The putative hydrolase gene bhp from the balhimycin biosynthetic gene cluster has been cloned and overexpressed in Escherichia coli. The corresponding enzyme Bhp was purified to homogeneity by nickel-chelating chromatography and characterized. Although Bhp has sequence similarities to hydrolases with "haloperoxidase"/perhydrolase activity, it did not show any enzymatic activity with standard "haloperoxidase"/perhydrolase substrates (e.g., monochlorodimedone and phenol red), nonspecific esterase substrates (such as p-nitrophenyl acetate, p-nitrophenyl phosphate and S-thiophenyl acetate) or the model lactonase substrate dihydrocoumarin. However, Bhp could be shown to catalyse the hydrolysis... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3080057 Tue, 08 Dec 2009 00:00:00 +0100 3080057 http://www.medworm.com/index.php?rid=3063877&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19967704%26dopt%3DAbstract Authors: Harwood KR, Miller SC PMID: 19967704 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3063877 Fri, 04 Dec 2009 00:00:00 +0100 3063877 http://www.medworm.com/index.php?rid=3063876&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19967705%26dopt%3DAbstract Authors: PMID: 19967705 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3063876 Fri, 04 Dec 2009 00:00:00 +0100 3063876 http://www.medworm.com/index.php?rid=3063875&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19967706%26dopt%3DAbstract Authors: PMID: 19967706 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3063875 Fri, 04 Dec 2009 00:00:00 +0100 3063875 http://www.medworm.com/index.php?rid=3057048&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19957260%26dopt%3DAbstract Authors: Labar G, Bauvois C, Borel F, Ferrer JL, Wouters J, Lambert DM 2-Arachidonoylglycerol plays a major role in endocannabinoid signaling, and is tightly regulated by the monoacylglycerol lipase (MAGL). Here we report the crystal structure of human MAGL. The protein crystallizes as a dimer, and despite structural homologies to haloperoxidases and esterases, it distinguishes itself by a wide and hydrophobic access to the catalytic site. An apolar helix covering the active site also gives structural insight into the amphitropic character of MAGL, and likely explains how MAGL interacts with membranes to recruit its substrate. Docking of 2-arachidonoylglycerol highlights a hydrophobic and a hydrophilic cavity that accommodate the lipid into the catalytic site. Moreover, we identified C... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3057048 Wed, 02 Dec 2009 00:00:00 +0100 3057048 http://www.medworm.com/index.php?rid=3048821&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19950344%26dopt%3DAbstract Authors: Thiagarajan V, Rajendran A, Satake H, Nishizawa S, Teramae N The binding behavior of green fluorescent ligands, derivatives of 7-nitrobenzo-2-oxa-1,3-diazole (NBD), with DNA duplexes containing an abasic (AP) site is studied by thermal denaturation and fluorescence experiments. Among NBD derivatives, N(1)-(7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)propane-1,3-diamine (NBD-NH(2)) is found to bind selectively to the thymine base opposite an AP site in a DNA duplex with a binding affinity of 1.52x10(6) M(-1). From molecular modeling studies, it is suggested that the NBD moiety binds to thymine at the AP site and a protonated amino group tethered to the NBD moiety interacts with the guanine base flanking the AP site. Green fluorescent NBD-NH(2) is successfully applied for simultaneous ... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3048821 Mon, 30 Nov 2009 00:00:00 +0100 3048821 http://www.medworm.com/index.php?rid=3048822&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19946930%26dopt%3DAbstract Authors: Prussia AJ, Yang Y, Geballe MT, Snyder JP Cyclostreptin (CS) is a recently discovered natural product with cytotoxic activity caused by microtubule stabilization. It is the only known microtubule-stabilizing agent (MSA) that covalently binds to tubulin. It also exhibits the fast-binding kinetics seen for other MSAs. Through careful peptide digestion and mass spectrometry analysis, Buey et al. found that two amino acids are labeled by CS: Asn228, near the known taxane-binding site, and Thr220, in the type I microtubule pore. This led Buey et al. to propose Thr220 resides at the site previously predicted to be a way station or low-affinity site. By using molecular dynamics simulations and structural considerations of the microtubule pore and tubulin dimer, we conclude that postu... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3048822 Fri, 27 Nov 2009 00:00:00 +0100 3048822 http://www.medworm.com/index.php?rid=3036335&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19943267%26dopt%3DAbstract Authors: Tamburro AM, Panariello S, Santopietro V, Bracalello A, Bochicchio B, Pepe A Resilin is a member of the family of elastomeric proteins and is found in specialised regions of the cuticle of most insects, and provides low stiffness, high strain and efficient energy storage. It is best known for its role in insect flight and the remarkable jumping ability of fleas and spittle bugs. In common with other elastomeric proteins, the recently identified Drosophila melanogaster proresilin shows glycine-rich repetitive sequences; in particular the N- and C-terminal regions of the protein are dominated by 18 repeats of a 15-residue sequence (SDTYGAPGGGNGGRP) and eleven repeats of a 13-residue sequence (GYSGGRPGGQDLG), respectively. We synthesised and analysed the molecular and supramolecu... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3036335 Thu, 26 Nov 2009 00:00:00 +0100 3036335 http://www.medworm.com/index.php?rid=3036334&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19943268%26dopt%3DAbstract We report the crystal structure of a thermophilic "ene" reductase (TOYE) isolated from Thermoanaerobacter pseudethanolicus E39. The crystal structure reveals a tetrameric enzyme and an active site that is relatively large compared to most other structurally determined and related Old Yellow Enzymes. The enzyme adopts higher order oligomeric states (octamers and dodecamers) in solution, as revealed by sedimentation velocity and multiangle laser light scattering. Bead modelling indicates that the solution structure is consistent with the basic tetrameric structure observed in crystallographic studies and electron microscopy. TOYE is stable at high temperatures (T(m)>70 degrees C) and shows increased resistance to denaturation in water-miscible organic solvents compared to the mesophilic O... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3036334 Thu, 26 Nov 2009 00:00:00 +0100 3036334 http://www.medworm.com/index.php?rid=3031124&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19937895%26dopt%3DAbstract Authors: Perrone L, Mothes E, Vignes M, Mockel A, Figueroa C, Miquel MC, Maddelein ML, Faller P Amyloid-beta peptides (Abeta) and the protein human serum albumin (HSA) interact in vivo. They are both localised in the blood plasma and in the cerebrospinal fluid. Among other functions, HSA is involved in the transport of the essential metal copper. Complexes between Abeta and copper ions have been proposed to be an aberrant interaction implicated in the development of Alzheimer's disease, where Cu is involved in Abeta aggregation and production of reactive oxygen species (ROS). In the present work, we studied copper-exchange reaction between Abeta and HSA or the tetrapeptide DAHK (N-terminal Cu-binding domain of HSA) and the consequence of this exchange on Abeta-induced ROS production an... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031124 Tue, 24 Nov 2009 00:00:00 +0100 3031124 http://www.medworm.com/index.php?rid=3031123&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19937896%26dopt%3DAbstract Authors: Mothes C, Larregola M, Quancard J, Goasdoué N, Lavielle S, Chassaing G, Lequin O, Karoyan P PMID: 19937896 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031123 Tue, 24 Nov 2009 00:00:00 +0100 3031123 http://www.medworm.com/index.php?rid=3031122&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19937897%26dopt%3DAbstract Authors: Fastrez J Enzymes and ribozymes constitute two classes of biological catalysts. The activity of many natural enzymes is regulated by the binding of ligands that have different structures than their substrates; these ligands are consequently called allosteric effectors. In most allosteric enzymes, the allosteric binding site lies far away from the active site. This implies that communication pathways must exist between these sites. While mechanisms of allosteric regulation were developed more than forty years ago, they continue to be revisited regularly. The improved understanding of these mechanisms has led in the past two decades to projects to transform several unregulated enzymes into allosterically regulated ones either by rational design or directed evolution techniques. ... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031122 Tue, 24 Nov 2009 00:00:00 +0100 3031122 http://www.medworm.com/index.php?rid=3031121&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19937900%26dopt%3DAbstract Authors: Dickens MG, Franz KJ PMID: 19937900 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031121 Tue, 24 Nov 2009 00:00:00 +0100 3031121 http://www.medworm.com/index.php?rid=3031120&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19938027%26dopt%3DAbstract Authors: Wéber E, Hetényi A, Váczi B, Szolnoki E, Fajka-Boja R, Tubak V, Monostori E, Martinek TA Galectin-1 (Gal-1), a ubiquitous beta-galactoside-binding protein expressed by various normal and pathological tissues, has been implicated in cancer and autoimmune/inflammatory diseases in consequence of its regulatory role in adhesion, cell viability, proliferation, and angiogenesis. The functions of Gal-1 depend on its affinity for beta-galactoside-containing glycoconjugates; accordingly, the inhibition of sugar binding blocks its functions, hence promising potential therapeutic tools. The Tyr-Xxx-Tyr peptide motifs have been reported to be glycomimetic sequences, mainly on the basis of their inhibitory effect on the Gal-1-asialofetuin (ASF) interaction. However, the resu... Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031120 Tue, 24 Nov 2009 00:00:00 +0100 3031120 http://www.medworm.com/index.php?rid=3031119&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19938028%26dopt%3DAbstract Authors: Ma L, Cockroft SL PMID: 19938028 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031119 Tue, 24 Nov 2009 00:00:00 +0100 3031119 http://www.medworm.com/index.php?rid=3031118&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19938030%26dopt%3DAbstract Authors: Sprinzl M, Erdmann VA PMID: 19938030 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031118 Tue, 24 Nov 2009 00:00:00 +0100 3031118 http://www.medworm.com/index.php?rid=3031127&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19937592%26dopt%3DAbstract Authors: Young JF, Nguyen HD, Yang L, Huskens J, Jonkheijm P, Brunsveld L PMID: 19937592 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031127 Fri, 20 Nov 2009 00:00:00 +0100 3031127 http://www.medworm.com/index.php?rid=3031126&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19937593%26dopt%3DAbstract Authors: Beshara CS, Jones CE, Daze KD, Lilgert BJ, Hof F PMID: 19937593 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031126 Fri, 20 Nov 2009 00:00:00 +0100 3031126 http://www.medworm.com/index.php?rid=3031125&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19937594%26dopt%3DAbstract Authors: Budin G, Dimala MM, Lamour V, Oudet P, Mioskowski C, Meunier S, Brino L, Wagner A PMID: 19937594 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3031125 Fri, 20 Nov 2009 00:00:00 +0100 3031125 http://www.medworm.com/index.php?rid=3008815&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19921671%26dopt%3DAbstract Authors: Scott DE, Dawes GJ, Ando M, Abell C, Ciulli A PMID: 19921671 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3008815 Tue, 17 Nov 2009 00:00:00 +0100 3008815 http://www.medworm.com/index.php?rid=3008814&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19921672%26dopt%3DAbstract Authors: Baigude H, Rana TM PMID: 19921672 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3008814 Tue, 17 Nov 2009 00:00:00 +0100 3008814 http://www.medworm.com/index.php?rid=3008813&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19921673%26dopt%3DAbstract Authors: PMID: 19921673 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3008813 Tue, 17 Nov 2009 00:00:00 +0100 3008813 http://www.medworm.com/index.php?rid=3008812&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19921674%26dopt%3DAbstract Authors: PMID: 19921674 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3008812 Tue, 17 Nov 2009 00:00:00 +0100 3008812 http://www.medworm.com/index.php?rid=3008816&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19918830%26dopt%3DAbstract Authors: Scism RA, Bachmann BO PMID: 19918830 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3008816 Fri, 13 Nov 2009 00:00:00 +0100 3008816 http://www.medworm.com/index.php?rid=3000414&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19911402%26dopt%3DAbstract Authors: Deiters A PMID: 19911402 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3000414 Thu, 12 Nov 2009 00:00:00 +0100 3000414 http://www.medworm.com/index.php?rid=3000413&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19911404%26dopt%3DAbstract Authors: Alemán EA, Pedini HS, Rueda D PMID: 19911404 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=3000413 Thu, 12 Nov 2009 00:00:00 +0100 3000413 http://www.medworm.com/index.php?rid=2982927&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19904793%26dopt%3DAbstract Authors: Bandow JE, Metzler-Nolte N PMID: 19904793 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2982927 Tue, 10 Nov 2009 00:00:00 +0100 2982927 http://www.medworm.com/index.php?rid=2982926&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19904794%26dopt%3DAbstract Authors: Bailey VJ, Keeley BP, Zhang Y, Ho YP, Easwaran H, Brock MV, Pelosky KL, Carraway HE, Baylin SB, Herman JG, Wang TH PMID: 19904794 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2982926 Tue, 10 Nov 2009 00:00:00 +0100 2982926 http://www.medworm.com/index.php?rid=2982925&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19904795%26dopt%3DAbstract Authors: Kim TS, Kim DR, Ahn HC, Shin D, Ahn DR PMID: 19904795 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2982925 Tue, 10 Nov 2009 00:00:00 +0100 2982925 http://www.medworm.com/index.php?rid=2977644&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19899089%26dopt%3DAbstract Authors: Green KD, Chen W, Houghton JL, Fridman M, Garneau-Tsodikova S Aminoglycosides are broad-spectrum antibiotics commonly used for the treatment of serious bacterial infections. Decades of clinical use have led to the widespread emergence of bacterial resistance to this family of drugs limiting their efficacy in the clinic. Here, we report the development of a methodology that utilizes aminoglycoside acetyltransferases (AACs) and unnatural acyl coenzyme A analogues for the chemoenzymatic generation of N-acylated aminoglycoside analogues. Generation of N-acylated aminoglycosides is followed by a simple qualitative test to assess their potency as potential antibacterials. The studied AACs (AAC(6')-APH(2'') and AAC(3)-IV) show diverse substrate promiscuity towards a variety of aminog... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2977644 Fri, 06 Nov 2009 00:00:00 +0100 2977644 http://www.medworm.com/index.php?rid=2977643&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19899090%26dopt%3DAbstract Authors: Kaya E, Gutsmiedl K, Vrabel M, Müller M, Thumbs P, Carell T PMID: 19899090 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2977643 Fri, 06 Nov 2009 00:00:00 +0100 2977643 http://www.medworm.com/index.php?rid=2977642&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19899094%26dopt%3DAbstract Authors: Swoboda JG, Campbell J, Meredith TC, Walker S PMID: 19899094 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2977642 Fri, 06 Nov 2009 00:00:00 +0100 2977642 http://www.medworm.com/index.php?rid=2959701&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885896%26dopt%3DAbstract Authors: García-Urdiales E, Busto E, Ríos-Lombardía N, Gotor-Fernández V, Gotor V The enantioselectivity displayed by the lipase from Pseudomonas cepacia towards a wide range of prochiral 2-substituted-propane-1,3-diamines was studied by means of molecular dynamics simulations (MDS). In all cases the enzyme allows the recovery of the corresponding amino carbamates of R configuration. However, the enantioselectivity is only synthetically useful if no ortho substituent is present and the aromatic ring is directly bonded to the 2-carbon of the 1,3-diamine core. Analysis of the MDS trajectories revealed that the homologation of 2-aryl substituents by means of a methylene group lowers enantioselectivity by alleviating the conformational tension of the slow-reacting orien... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2959701 Mon, 02 Nov 2009 00:00:00 +0100 2959701 http://www.medworm.com/index.php?rid=2959700&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19885899%26dopt%3DAbstract Authors: Kormann C, Pimenta I, Löber S, Wimmer C, Lanig H, Clark T, Hillen W, Gmeiner P Synthesis, biological investigations and molecular docking studies of nonantibiotic and nontetracyclic inducers that feature a minimal key motif of the natural lead tetracycline are presented. The diarylpropane-1,3-dione motif was identified as the minimal substructure responsible for TetR induction by tetracyclines. The first nontetracyclic surrogates of the natural tetracyclines displayed significant inducing effects for TetR(BD)S135L, whereby the chlorohydroxyphenyl-substituted beta-diketone 31 displayed the highest activity. Interestingly, antibiotic activity could not be detected for 31. Homology modeling based on the X-ray structure of 7-chlorotetracycline bound to TetR indicated analogou... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2959700 Mon, 02 Nov 2009 00:00:00 +0100 2959700 http://www.medworm.com/index.php?rid=2959702&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19882698%26dopt%3DAbstract Authors: Raber ML, Castillo A, Greer A, Townsend CA beta-Lactam synthetase (beta-LS) is the paradigm of a growing class of enzymes that form the critical beta-lactam ring in the clavam and carbapenem antibiotics. beta-LS catalyzes a two-stage reaction in which N(2)-(2-carboxyethyl)-L-arginine is first adenylated, and then undergoes intramolecular ring closure. It was previously shown that the forward kinetic commitment to beta-lactam formation is high, and that the overall rate of reaction is partially limited to a protein conformational change rather than to the chemical step alone of closing the strained ring. beta-Lactam formation was evaluated on the basis of X-ray crystal structures, site-specific mutation, and kinetic and computational studies. The combined evidence clearly point... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2959702 Fri, 30 Oct 2009 00:00:00 +0100 2959702 http://www.medworm.com/index.php?rid=2947745&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19877000%26dopt%3DAbstract Authors: Faller P The metal ions copper, zinc and iron have been shown to be involved in Alzheimer's disease (AD). Cu, Zn and Fe ions are proposed to be implicated in two key steps of AD pathology: 1) aggregation of the peptide amyloid-beta (Abeta), and 2) production of reactive oxygen species (ROS) induced by Abeta. There is compelling evidence that Cu and Zn bind directly to Abeta in AD. This formation of Cu/Zn-Abeta complexes is thought to be aberrant as they have been detected only in AD, but not under healthy conditions. In this context, the understanding of how these metal ions interact with Abeta, their influence on structure and oligomerization become an important issue for AD. Moreover, the mechanism of ROS production by Cu-Abeta in relation to its aggregations state, as well ... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2947745 Wed, 28 Oct 2009 00:00:00 +0100 2947745 http://www.medworm.com/index.php?rid=2947744&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19877001%26dopt%3DAbstract Authors: Erbe A, Kerth A, Dathe M, Blume A Interactions of the cationic amphipathic peptide KLALKLALKALKAALKLA-NH(2) (KLAL) and its double D-amino acid replacement analogues l(11)k(12)-KLAL and k(9)a(10)-KLAL with lipid monolayers of anionic POPG, zwitterionic POPC and mixtures thereof at the air/water interface were investigated by infrared reflection- absorption spectroscopy (IRRAS). At high surface pressure (>30 mN m(-1)) all peptides incorporated into lipid monolayers containing at least 25 % anionic POPG, and adopted an alpha-helical conformation. Creation of free surface by expansion of the monolayers resulted in an additional adsorption of peptides from the subphase, but now in a beta-sheet conformation; this led to the coexistence of peptides in two distinctly different conf... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2947744 Wed, 28 Oct 2009 00:00:00 +0100 2947744 http://www.medworm.com/index.php?rid=2947743&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19877002%26dopt%3DAbstract Authors: Harwood KR, Miller SC PMID: 19877002 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2947743 Wed, 28 Oct 2009 00:00:00 +0100 2947743 http://www.medworm.com/index.php?rid=2935144&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19859931%26dopt%3DAbstract Authors: Salwiczek M, Koksch B PMID: 19859931 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2935144 Mon, 26 Oct 2009 00:00:00 +0100 2935144 http://www.medworm.com/index.php?rid=2935151&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19856336%26dopt%3DAbstract Authors: Clerc J, Florea BI, Kraus M, Groll M, Huber R, Bachmann AS, Dudler R, Driessen C, Overkleeft HS, Kaiser M PMID: 19856336 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2935151 Fri, 23 Oct 2009 00:00:00 +0100 2935151 http://www.medworm.com/index.php?rid=2935150&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19856338%26dopt%3DAbstract Authors: PMID: 19856338 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2935150 Fri, 23 Oct 2009 00:00:00 +0100 2935150 http://www.medworm.com/index.php?rid=2935149&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19856345%26dopt%3DAbstract Authors: PMID: 19856345 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2935149 Fri, 23 Oct 2009 00:00:00 +0100 2935149 http://www.medworm.com/index.php?rid=2935148&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19856365%26dopt%3DAbstract Authors: Barluenga S, Fontaine JG, Wang C, Aouadi K, Chen R, Beebe K, Neckers L, Winssinger N The pochoximes, based on the radicicol pharmacophore, are potent inhibitors of heat shock protein 90 (HSP90) that retain their activity in vivo. Herein we report an extended library that broadly explores the structure-activity relationship (SAR) of the pochoximes with four points of diversity. Several modifications were identified that afford improved cellular efficacy, new opportunities for conjugation, and further diversifications. Cocrystal structures of pochoximes A and B with HSP90 show that pochoximes bind to a different conformation of HSP90 than radicicol and provide a rationale for the enhanced affinity of the pochoximes relative to radicicol and the pochonins. PMID: 19856365 [Pub... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2935148 Fri, 23 Oct 2009 00:00:00 +0100 2935148 http://www.medworm.com/index.php?rid=2935147&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19856367%26dopt%3DAbstract Authors: Wollack JW, Silverman JM, Petzold CJ, Mougous JD, Distefano MD Recently a number of nonnatural prenyl groups containing alkynes and azides have been developed as handles to perform click chemistry on proteins and peptides ending in the sequence "CAAX", where C is a cysteine that becomes alkylated, A is an aliphatic amino acid and X is any amino acid. When such molecules are modified, a tag containing a prenyl analogue and the "CAAX box" sequence remains. Here we report the synthesis of an alkyne-containing substrate comprised of only nine nonhydrogen atoms. This substrate was synthesized in six steps from 3-methylbut-2-en-1-ol and has been enzymatically incorporated into both proteins and peptides by using protein farnesyltransferase. After prenylation the final three amino ac... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2935147 Fri, 23 Oct 2009 00:00:00 +0100 2935147 http://www.medworm.com/index.php?rid=2935146&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19856369%26dopt%3DAbstract Authors: Parameswar AR, Park IH, Saksena R, Kováč P, Nahm MH, Demchenko AV The first synthesis of the newly discovered oligosaccharide of pneumococcal serotype 6C and its spacer-containing analogue is reported. Conjugation of the spacer-containing oligosaccharides of pneumococcal saccharides 6A, 6B, 6C and derivatives thereof with bovine serum albumin (BSA) protein carrier was carried out by using squaric-acid approach to obtain the oligosaccharide-protein conjugates in excellent yields. The conjugates have been tested with a rabbit antiserum pool (Pool B) used for pneumococcal serotyping. The results showed that synthetic carbohydrate conjugates express epitopes found in native capsular polysaccharides of serotypes 6A, 6B, and 6C. PMID: 19856369 [PubMed - as supplied by... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2935146 Fri, 23 Oct 2009 00:00:00 +0100 2935146 http://www.medworm.com/index.php?rid=2935145&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19856370%26dopt%3DAbstract Authors: Freiburger LA, Auclair K, Mittermaier AK PMID: 19856370 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2935145 Fri, 23 Oct 2009 00:00:00 +0100 2935145 http://www.medworm.com/index.php?rid=2923882&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19852013%26dopt%3DAbstract Authors: Diestel R, Irschik H, Jansen R, Khalil MW, Reichenbach H, Sasse F Chivosazoles A and F, isolated from Sorangium cellulosum, showed high antiproliferative activity with different mammalian cell lines including human cancer cells. The chivosazoles caused a delay in G2/M phase of the cell cycle, and treated cells often contained two nuclei. By labeling F-actin it was shown that the actin cytoskeleton of the cells starts to break down after a few minutes of treatment. In vitro polymerization assays with purified G-actin revealed that the chivosazoles inhibit actin polymerization and also cause a depolymerization of pyrene-labeled F-actin microfilaments prepared in vitro. Chivosazoles are new tools for the investigation of issues concerning the actin cytoskeleton and they have a di... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2923882 Thu, 22 Oct 2009 00:00:00 +0100 2923882 http://www.medworm.com/index.php?rid=2923884&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19847841%26dopt%3DAbstract Authors: Branco RJ, Graber M, Denis V, Pleiss J Hydration is a major determinant of activity and selectivity of enzymes in organic solvents or in gas phase. The molecular mechanism of the hydration of Candida antarctica lipase B (CALB) and its dependence on the thermodynamic activity of water (a(w)) was studied by molecular dynamics simulations and compared to experimentally determined water sorption isotherms. Hydration occurred in two phases. At low water activity, single water molecules bound to specific water binding sites at the protein surface. As the water activity increased, water networks gradually developed. The number of protein-bound water molecules increased linearly with a(w), until at a(w)=0.5 a spanning water network was formed consisting of 311 water molecules, which c... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2923884 Wed, 21 Oct 2009 00:00:00 +0100 2923884 http://www.medworm.com/index.php?rid=2923883&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19847842%26dopt%3DAbstract Authors: Schließmann A, Hidalgo A, Berenguer J, Bornscheuer UT Four hydrophobic and bulky amino acid residues (F126, F144, F159, and I225) were identified to form a bottleneck guarding the entrance to the active site of an esterase from Pseudomonas fluorescens (PFE I). Hence, a range of nonpolar amino acids were introduced into PFE I to broaden the substrate range and to increase enantioselectivity while preserving the hydrophobicity of the tunnel. First, single variants were created and then the most enantioselective ones were combined to find cooperative effects. This resulted in several mutants, which showed substantially enhanced enantioselectivity; for instance, in the kinetic resolution of 1-phenyl-1-propyl acetate, with which the wild type only showed E=1.2, two mutants gav... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2923883 Wed, 21 Oct 2009 00:00:00 +0100 2923883 http://www.medworm.com/index.php?rid=2901971&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19834928%26dopt%3DAbstract In this study, we suppressed this effect by reducing the length of the original template derived from the Tetrahymena intron RNA. This modification, however, decreased the template ability for the specific reaction. As an alternative RNA that was as effective as the original template, we found that a self-dimerizing RNA was a promising template for peptide ligation without a nonspecific effect. PMID: 19834928 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2901971 Wed, 14 Oct 2009 23:00:00 +0100 2901971 http://www.medworm.com/index.php?rid=2901974&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830760%26dopt%3DAbstract Authors: Luan Z, Higaki K, Aguilar-Moncayo M, Ninomiya H, Ohno K, García-Moreno MI, Ortiz Mellet C, García Fernández JM, Suzuki Y Gaucher disease (GD), the most prevalent lysosomal storage disorder, is caused by mutations of lysosomal beta-glucosidase (acid beta-Glu, beta-glucocerebrosidase); these mutations result in protein misfolding. Some inhibitors of this enzyme, such as the iminosugar glucomimetic N-(n-nonyl)-1-deoxynojirimycin (NN-DNJ), are known to bind to the active site and stabilize the proper folding for the catalytic form, acting as "chemical chaperones" that facilitate transport and maturation of acid beta-Glu. Recently, bicyclic nojirimycin (NJ) analogues with structure of sp(2) iminosugars were found to behave as very selective, competitive inhibitors of... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2901974 Tue, 13 Oct 2009 23:00:00 +0100 2901974 http://www.medworm.com/index.php?rid=2901973&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830761%26dopt%3DAbstract Authors: Schneider P, Jacobs JM, Neres J, Aldrich CC, Allen C, Nett M, Hoffmeister D PMID: 19830761 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2901973 Tue, 13 Oct 2009 23:00:00 +0100 2901973 http://www.medworm.com/index.php?rid=2901972&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19830762%26dopt%3DAbstract Authors: Diederichsen U PMID: 19830762 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2901972 Tue, 13 Oct 2009 23:00:00 +0100 2901972 http://www.medworm.com/index.php?rid=2895026&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19827080%26dopt%3DAbstract Authors: Scott DE, Dawes GJ, Ando M, Abell C, Ciulli A A new strategy that combines the concepts of fragment-based drug design and dynamic combinatorial chemistry (DCC) for targeting adenosine recognition sites on enzymes is reported. We demonstrate the use of 5'-deoxy-5'-thioadenosine as a noncovalent anchor fragment in dynamic combinatorial libraries templated by Mycobacterium tuberculosis pantothenate synthetase. A benzyl disulfide derivative was identified upon library analysis by HPLC. Structural and binding studies of protein-ligand complexes by X-ray crystallography and isothermal titration calorimetry informed the subsequent optimisation of the DCC hit into a disulfide containing the novel meta-nitrobenzyl fragment that targets the pantoate binding site of pantothenate syntheta... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2895026 Mon, 12 Oct 2009 23:00:00 +0100 2895026 http://www.medworm.com/index.php?rid=2881449&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19816890%26dopt%3DAbstract Authors: Lafaquière V, Barbe S, Puech-Guenot S, Guieysse D, Cortés J, Monsan P, Siméon T, André I, Remaud-Siméon M Lipase from Burkholderia cepacia (BCL) has proven to be a very useful biocatalyst for the resolution of 2-substituted racemic acid derivatives, which are important chiral building blocks. Our previous work showed that enantioselectivity of the wild-type BCL could be improved by chemical engineering of the substrate's molecular structure. From this earlier study, three amino acids (L17, V266 and L287) were proposed as targets for mutagenesis aimed at tailoring enzyme enantioselectivity. In the present work, a small library of 57 BCL single mutants targeted on these three residues was constructed and screened for enantioselectivity towards (R,S)-2-ch... Chembiochem journals http://www.medworm.com/rss/comments.php?id=2881449 Wed, 07 Oct 2009 23:00:00 +0100 2881449 http://www.medworm.com/index.php?rid=2881448&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19816891%26dopt%3DAbstract Authors: Loveridge EJ, Maglia G, Allemann RK PMID: 19816891 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2881448 Wed, 07 Oct 2009 23:00:00 +0100 2881448 http://www.medworm.com/index.php?rid=2876987&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19813201%26dopt%3DAbstract Authors: Hauch Fenger T, Bjerre J, Bols M PMID: 19813201 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2876987 Tue, 06 Oct 2009 23:00:00 +0100 2876987 http://www.medworm.com/index.php?rid=2876986&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19813202%26dopt%3DAbstract Authors: Nakano C, Hoshino T PMID: 19813202 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2876986 Tue, 06 Oct 2009 23:00:00 +0100 2876986 http://www.medworm.com/index.php?rid=2876985&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19813204%26dopt%3DAbstract Authors: PMID: 19813204 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2876985 Tue, 06 Oct 2009 23:00:00 +0100 2876985 http://www.medworm.com/index.php?rid=2876984&cid=s_37781_60_f&fid=37781&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fentrez%2Fquery.fcgi%3Ftmpl%3DNoSidebarfile%26db%3DPubMed%26cmd%3DRetrieve%26list_uids%3D19813205%26dopt%3DAbstract Authors: PMID: 19813205 [PubMed - as supplied by publisher] (Source: Chembiochem) Chembiochem journals http://www.medworm.com/rss/comments.php?id=2876984 Tue, 06 Oct 2009 23:00:00 +0100 2876984